LOCUS       DASWLN010000001         2110 bp    DNA     linear   ENV 20-FEB-2024
DEFINITION  MAG TPA_asm: Candidatus Zixiibacteriota bacterium isolate
            SMAG_U2949 k141_254851, whole genome shotgun sequence.
ACCESSION   DASWLN010000001 DASWLN010000000
VERSION     DASWLN010000001.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37960594
            Sequence Read Archive: SRR10376091
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Candidatus Zixiibacteriota bacterium (soil metagenome)
  ORGANISM  Candidatus Zixiibacteriota bacterium
            Bacteria.
REFERENCE   1  (bases 1 to 2110)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 2110)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (26-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/12/2024 08:28:26
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 1,440
            CDSs (total)                      :: 1,416
            Genes (coding)                    :: 1,412
            CDSs (with protein)               :: 1,412
            Genes (RNA)                       :: 24
            rRNAs                             :: 1 (16S)
            partial rRNAs                     :: 1 (16S)
            tRNAs                             :: 21
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 4
            CDSs (without protein)            :: 4
            Pseudo Genes (ambiguous residues) :: 0 of 4
            Pseudo Genes (frameshifted)       :: 0 of 4
            Pseudo Genes (incomplete)         :: 3 of 4
            Pseudo Genes (internal stop)      :: 1 of 4
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2110
                     /organism="Candidatus Zixiibacteriota bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_254851"
                     /isolate="SMAG_U2949"
                     /isolation_source="soil"
                     /db_xref="taxon:2053527"
                     /environmental_sample
                     /country="USA: Oregon"
                     /lat_lon="42.99 N 123.63 W"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            <1..270
                     /locus_tag="VGB16_00005"
     CDS             <1..270
                     /locus_tag="VGB16_00005"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_014808219.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="TRAP transporter large permease subunit"
                     /protein_id="HEX9916097.1"
                     /translation="ATPIFYPVVLKLGFDPIWFGVILHIFVMIGVVIPPVAVNVFVVK
                     NITKEPFGVIYSGVYPFIIGMILCAALLFMFPQICLYLPHKLSLM"
     gene            317..1867
                     /locus_tag="VGB16_00010"
     CDS             317..1867
                     /locus_tag="VGB16_00010"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_015359342.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005524 - ATP binding [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="class I adenylate-forming enzyme family protein"
                     /protein_id="HEX9916098.1"
                     /translation="MNMVSIIVKNARIYPNEIAFVEVKPVTYVRREISWAQFSERVNR
                     LANALIDRGVKKGEKVFIYGRNSINWLEAYFAVMATGAWALPLNFRFTGDDIKYCADV
                     AQPVAFILDEEYTEIISAIRPDLTAVRNYVCIGSFKDMERMEDLIGKSSPKLPKVEIK
                     GEDECALYFTSGTTGPPKPVLHVHKNMVGVAINEVINDRWEHSDTLLMMPPFYHLAIG
                     HLFGCMLAGGRAILLTEKITPQYIFENISKEQISIVFLLVPWALDILEALDKGELRKE
                     NYDLRHWRLMFMGAQPIPPNLVQRWKKYFPEMQFDNTYGLSESAGPGTVHLGIGNERK
                     IGAIGKPGLLWDARIVNDHGEDVRLGEIGELIIKGPSVMKEYYKNPQLTAQTIQDDWL
                     YTGDLAKMDEEGFIYLVDRKKDLIISGGENIYPIEVEEVIQRHPKVRDVAAIGTPDER
                     LGEVVTAFIELKAGEALTEEEMKSFCEQNLPRYKRPRRIIFDQIPRSSTGKTEKRKLR
                     EKYLKLKQ"
BASE COUNT          604 a          403 c          518 g          585 t
ORIGIN      
        1 gccactccca tcttctatcc tgtcgtcctc aaactcggat tcgatcctat atggtttggt
       61 gtcattctgc atatttttgt aatgatcggg gtggttattc ctcctgtggc ggttaatgtc
      121 tttgtggtca aaaatattac caaggagcca tttggcgtga tttatagcgg tgtctatcct
      181 tttataatcg gtatgatcct ttgtgcagct ttattgttta tgtttccaca gatctgcctt
      241 tatcttcccc ataaattatc actgatgtag ggtttgactt gtctggagcg aaaaggaata
      301 tttgggagag attactatga atatggtgtc catcatagtc aagaatgctc gaatctatcc
      361 taatgagata gcgtttgttg aggtaaaacc tgtcacctat gtgagaaggg aaatcagttg
      421 ggctcaattt agtgagagag tcaatcgatt ggccaatgcc cttatagata gaggagtgaa
      481 aaaaggcgag aaggttttta tttatgggag aaattccata aactggcttg aagcttattt
      541 tgctgtcatg gcaacgggag catgggccct tcctctcaat tttagattta ccggtgatga
      601 tataaagtat tgtgctgacg tagctcagcc agtggctttt attctggatg aagaatatac
      661 agagataata agcgctattc gtccggactt aactgcggta agaaactatg tttgcattgg
      721 ctcttttaaa gacatggagc gtatggagga tcttattgga aaaagctctc ccaagctccc
      781 taaggtagag ataaagggtg aggatgagtg tgcactttat ttcacctcag gcaccaccgg
      841 tcctcccaag ccggtactcc atgtccacaa gaatatggtt ggtgttgcca taaatgaagt
      901 aatcaatgat cggtgggagc acagtgacac ccttttgatg atgccccctt tctatcattt
      961 ggccatagga catttatttg gatgcatgct tgcgggagga agagccatcc tgttgacaga
     1021 aaagattacg ccgcagtaca tatttgaaaa catttcgaag gagcagatat ccatcgtatt
     1081 tctcctggta ccgtgggctt tagacattct tgaggcttta gataaaggag aattaaggaa
     1141 ggagaattat gatctcagac attggcgtct gatgttcatg ggtgcccagc ctattcctcc
     1201 caacttagtt caaaggtgga agaaatattt cccggagatg caatttgata atacctatgg
     1261 gttgagtgaa tctgcagggc caggaacggt tcaccttggg attggaaacg agaggaaaat
     1321 tggcgctatt ggaaaaccag gcttattgtg ggatgctcgt atcgtcaatg accatggtga
     1381 agatgtacga ctaggagaga ttggcgaact cattatcaaa ggtcctagcg tcatgaagga
     1441 atattataaa aatccccaat taaccgccca gactatacaa gacgattggc tatacacagg
     1501 tgatttagcc aaaatggatg aagaaggatt tatttacctt gtcgatagaa aaaaggactt
     1561 aatcattagc ggtggggaaa acatttatcc catagaggtc gaagaggtaa ttcagaggca
     1621 ccctaaggtt cgtgatgtgg cagcgatagg gactccagac gagagattag gcgaagtcgt
     1681 tacggctttc attgagctta aggctgggga ggctttaact gaagaagaaa tgaagtcatt
     1741 ctgtgaacag aacttacccc gttacaagcg ccctcgccgg attatctttg accaaatacc
     1801 gcgaagctcc actggcaaga cagaaaagcg gaagctgaga gagaaatacc ttaagctaaa
     1861 acagtgatca gggatagtgc cattttatta tatatatttc ctcagcgccg cgtcgccgca
     1921 accaaatgaa aagatgtctc acgcagagac cgcggagagt gaaatttctc tccaatcggt
     1981 atctcccgat tggaggaaaa ggttgctttt atctgagttc tctctggact cgagcgaagc
     2041 ccacgaacct ctaacatggt tcggggcagg cgggcgtgag ataaaaaata aaacatgcaa
     2101 agatatatcg
//
LOCUS       DASWLN010000002        12621 bp    DNA     linear   ENV 20-FEB-2024
DEFINITION  MAG TPA_asm: Candidatus Zixiibacteriota bacterium isolate
            SMAG_U2949 k141_258867, whole genome shotgun sequence.
ACCESSION   DASWLN010000002 DASWLN010000000
VERSION     DASWLN010000002.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37960594
            Sequence Read Archive: SRR10376091
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Candidatus Zixiibacteriota bacterium (soil metagenome)
  ORGANISM  Candidatus Zixiibacteriota bacterium
            Bacteria.
REFERENCE   1  (bases 1 to 12621)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 12621)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (26-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/12/2024 08:28:26
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 1,440
            CDSs (total)                      :: 1,416
            Genes (coding)                    :: 1,412
            CDSs (with protein)               :: 1,412
            Genes (RNA)                       :: 24
            rRNAs                             :: 1 (16S)
            partial rRNAs                     :: 1 (16S)
            tRNAs                             :: 21
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 4
            CDSs (without protein)            :: 4
            Pseudo Genes (ambiguous residues) :: 0 of 4
            Pseudo Genes (frameshifted)       :: 0 of 4
            Pseudo Genes (incomplete)         :: 3 of 4
            Pseudo Genes (internal stop)      :: 1 of 4
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..12621
                     /organism="Candidatus Zixiibacteriota bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_258867"
                     /isolate="SMAG_U2949"
                     /isolation_source="soil"
                     /db_xref="taxon:2053527"
                     /environmental_sample
                     /country="USA: Oregon"
                     /lat_lon="42.99 N 123.63 W"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            <1..882
                     /locus_tag="VGB16_00015"
     CDS             <1..882
                     /locus_tag="VGB16_00015"
                     /inference="COORDINATES: protein motif:HMM:NF024299.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="tetratricopeptide repeat protein"
                     /protein_id="HEX9916099.1"
                     /translation="LAREHNLDPINIKDMGEMEEKAWGDIFSKGVNLANQDSSEEALK
                     WFKLAISIKPEKYDGYLNYCVTSAKMDKITEALPYCDKAYQLMPEDGNVQNIYAFVLV
                     NAKQYDKALGIYNKILERDPTNINALTNLAMIYGQKGDDSNAMQVYNKILQVDPEQKD
                     TYFNRGLVFMKQAQAYYEQMKQANDALAADPQNKTLEAKVRQLLAEQKEIYHKAELDF
                     AKAMELEPNDNEARGYLGGSLFHQEKWDEALQVFEKLVSLDPNNKEAWNYLAVLYTKK
                     GMKEKGEEALKKAGITQ"
     gene            958..3162
                     /gene="priA"
                     /locus_tag="VGB16_00020"
     CDS             958..3162
                     /gene="priA"
                     /locus_tag="VGB16_00020"
                     /inference="COORDINATES: protein motif:HMM:TIGR00595.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0005658 - alpha DNA polymerase:primase
                     complex [Evidence IEA];
                     GO_function: GO:0003678 - DNA helicase activity [Evidence
                     IEA];
                     GO_function: GO:0003688 - DNA replication origin binding
                     [Evidence IEA];
                     GO_function: GO:0016818 - hydrolase activity, acting on
                     acid anhydrides, in phosphorus-containing anhydrides
                     [Evidence IEA];
                     GO_process: GO:0006260 - DNA replication [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="primosomal protein N'"
                     /protein_id="HEX9916100.1"
                     /translation="MYVDLALPLPIKKLFTYGVPESLVGKVYLGQRVLVQLGKRLMMG
                     VVVGLNVPAPQIKTKNILLALETYPWFSNTVLGLTKWMSEYYSADWGECLKFASPQYP
                     DISGRIWIENVPADLEMKGLSAEEKDTLGLIKSKQKMKLNRLVQSSGMEDIYQHLQHL
                     QQKGLVRFGLRPIKTDPKDPALPLRIRAKSSSTEISGLGLKDDERKIVDLISQDIEKK
                     TYSTFLLMGRDRITRRMVHLELAKKAVQAQKQVLVILPETHLLDETVSSWQRTFNEKM
                     AVLHPQISINQKMGIWEKIKSKEFSLVLGTRSAVFAPLDDLGLIVVEEENHPLHKEEA
                     SPHYNARDVAVVRGRLEKATVILSCSFPSLESFYNCQRGKYRLCDIRVESKARPALKT
                     EIIDKKKSGAKAIISPPVLDILRKETKPVVLLLNRRGSSRLVICEDCGLVFRCPDCGI
                     SLALHKDESVLRCRYCNYEQKGANVCPQCGGSDFSYRGLGTQDLEQFLSESLPQIKSV
                     RLDKDVTDKPGGAKRILFEFYQGKYQVLLGTQMIFGGLDLSKVGLVIILSIDELLTYP
                     DFRARERTFHFLSQIVDRMIEMNSQAKLVLQTSRPKDWVIRCALRGDFDYFYQKEIEQ
                     RKELFYPPFSHLISIWFSGRIQEKAMKFSSSFVQSLNRIIEQGKVSKTKILGPTPIYH
                     AKRKKPNQWQLIIKTTSPMKVNNLTNSILKRDEFAKNRSVRIKVEVDPLSLV"
     gene            3167..3670
                     /locus_tag="VGB16_00025"
     CDS             3167..3670
                     /locus_tag="VGB16_00025"
                     /inference="COORDINATES: protein motif:HMM:NF012579.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:1902495 - transmembrane transporter
                     complex [Evidence IEA];
                     GO_function: GO:0016301 - kinase activity [Evidence IEA];
                     GO_process: GO:0008643 - carbohydrate transport [Evidence
                     IEA];
                     GO_process: GO:0009401 - phosphoenolpyruvate-dependent
                     sugar phosphotransferase system [Evidence IEA];
                     GO_process: GO:0016310 - phosphorylation [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="PTS sugar transporter subunit IIA"
                     /protein_id="HEX9916101.1"
                     /translation="MQISKLLKPELIKLEMKTKVEFPADSSVHPQKRMMFIKNEILKE
                     LVDLLDKSGRVVNKKKLLLDFFNREKKATTGIGQGIAIPHIRSMQAREFILGFARSKS
                     GYEFDSLDKKPVHLFFVMAAPPYEDKLYLKIFKALAGMLQYDYFIQKLLKVSTEYEVI
                     RAIEEME"
     gene            3933..4436
                     /locus_tag="VGB16_00030"
     CDS             3933..4436
                     /locus_tag="VGB16_00030"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_013788650.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="rubrerythrin family protein"
                     /protein_id="HEX9916102.1"
                     /translation="MRKMTQDNLNSAFAGESKAHMRYLIFSQCAQKEGKPNTSRLFKA
                     IAFAEQVHAANHFKVLGELGTTSQNLGAATDGEDFEIEEMYPAYLEVAKLQDEKTAQK
                     SMNYALQAEKIHCAMYQKAKQSIDEGKDASLKDIYICGVCGYTVEGEIPEACPVCKAT
                     TDKFKKF"
     gene            4483..4638
                     /locus_tag="VGB16_00035"
     CDS             4483..4638
                     /locus_tag="VGB16_00035"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_015411233.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEX9916103.1"
                     /translation="MPAKKRKTARKAKSAYVCVPCGAEITVTKEGMGTSTLMCCGQVM
                     KSKAKKR"
     gene            4922..5374
                     /locus_tag="VGB16_00040"
     CDS             4922..5374
                     /locus_tag="VGB16_00040"
                     /inference="COORDINATES: protein motif:HMM:NF014912.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ferritin family protein"
                     /protein_id="HEX9916104.1"
                     /translation="MDSCVFNEVIVFAARKEAESANLYQVYANLASASNVKNMFKEMR
                     REELKHQKLLENIKLKDISAYKLKDIPDLKIGDYSTQGEFSPDMKYAEALMLAIKKEE
                     MSYKLYQDLAFSSKDEDLKKLFLVLVSEEAKHKLKLEEEYDKTVTSEN"
     gene            5377..6369
                     /locus_tag="VGB16_00045"
     CDS             5377..6369
                     /locus_tag="VGB16_00045"
                     /inference="COORDINATES: protein motif:HMM:NF018884.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016791 - phosphatase activity [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="SpoIIE family protein phosphatase"
                     /protein_id="HEX9916105.1"
                     /translation="MRKSKEFLNILLGAMARETEAFNYYNEASQSSPYDETKALLVQL
                     SLEEKRHREILAGEYGRIKRLIFSKGKEYIKREKISFAIPNELPYLRVRTIPGIKAAA
                     IDLPSRFLGGDYVDTFPITFGKKSLLGVVIYDVMGHGIDATGLKAVTRKTLDRIREAL
                     REEEILNLANPAWLVSQVNSELWRECRQRGSFVSLLYMVIDRIGRKLTYTSAGHQPAV
                     LVRRKNGDSETLSDSELLVGVEGEREYTQSSLDFSRGDLVLLFTDGVVETLNTKQEEY
                     GTKRLVELLKKNSQLDLAELLLEICQDLKKFNRGKNFKDEVSMIALKIEKEKNK"
     gene            6366..6893
                     /locus_tag="VGB16_00050"
     CDS             6366..6893
                     /locus_tag="VGB16_00050"
                     /inference="COORDINATES: protein motif:HMM:NF014912.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ferritin family protein"
                     /protein_id="HEX9916106.1"
                     /translation="MKKDKENLPSEARGQVPENMTPLEILKLAIYDEQSACDFYSYLS
                     GQIRNQSGKEKFKFLAEDEKRHRSILEKEYQKESKGRKFGFDPKKAKTIRVKVDGESS
                     ASSALDLAIQAERGAYKFYTSAAEKVKSQSSKSMFLRLAEEEDRHYNLLAAERQALSS
                     NFYWYGYDVPGVMEE"
     gene            6908..7066
                     /locus_tag="VGB16_00055"
     CDS             6908..7066
                     /locus_tag="VGB16_00055"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_014063361.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="rubredoxin"
                     /protein_id="HEX9916107.1"
                     /translation="MDKWQCQVCGYIYDPHKGDPENGIEVGTSFERLPDEWVCPECGA
                     AKDQFEKI"
     gene            7101..8297
                     /locus_tag="VGB16_00060"
     CDS             7101..8297
                     /locus_tag="VGB16_00060"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_015311330.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="FprA family A-type flavoprotein"
                     /protein_id="HEX9916108.1"
                     /translation="MEAPRVKEGVWWVGAEDKDLRIFDIVIPTAYGTTYNSYLIKGEK
                     IALVEGVKKGFQEQLFSHIKRVCNLEDIRYLIINHTEPDHSGCIAELIDLVPNITLVY
                     SKTAKTFVENIIHREADSIVVEDDEELDLGGKTLKFISAPFLHWPDTMFTYLVEDEIL
                     FPCDCFGAHYCGENIFNDQLKSPQSAFGSFKHYYDHIFRPFKSYVLRALEKIEHLKID
                     IIAPSHGPILRTDPKSYLEWYKRWSEFPTSDDKKRITIVYVSAYGNTQRMAKHIANGL
                     KQKGVEVSLLDATQIDMDTIINKIEASDGIIFGTPTLNAKAPKPILDVVSNLVLLNIR
                     GKSAAVFGSYGWSGEAVGMVEEISKSIGFRIREPGLKVKLVPTAQDDKICEEFGSKFA
                     ESLTAK"
     gene            8449..8862
                     /locus_tag="VGB16_00065"
     CDS             8449..8862
                     /locus_tag="VGB16_00065"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_012814463.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF559 domain-containing protein"
                     /protein_id="HEX9916109.1"
                     /translation="MDKRFFLRFPFEKGGLRGILEEMLKYNKNLKSLSRELRNNMTDS
                     EKLLWGKIRRKQLKSYQFYRQRTIGDYIVDFYCPKAGLIIEVDGGQHYSDKGKRKDKI
                     RDVCMESLGMRVLRFSDKKIFGNLEGVLKEIGSYL"
     gene            complement(9959..>12621)
                     /locus_tag="VGB16_00070"
     CDS             complement(9959..>12621)
                     /locus_tag="VGB16_00070"
                     /inference="COORDINATES: protein motif:HMM:NF036531.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004866 - endopeptidase inhibitor activity
                     [Evidence IEA]"
                     /codon_start=3
                     /transl_table=11
                     /product="alpha-2-macroglobulin family protein"
                     /protein_id="HEX9916110.1"
                     /translation="ALMYDRSLEYYAKSDNLWLDDLYALRTTPIYWQYSVFNPNVYSL
                     PITEGLLEKLLKAFRQPSGEPKLPGLRTWETWANGDYRGGGGGKLLKEQVGFAYNGYA
                     PAADAISSVSEKKDKSEFEHQEAGAGIKTRRKFADTAFFKPHIVTNILGNGRFSFVSP
                     EQLTSWRIKVLAFTKDAKQGTLTEEVVTKKELMVRADLPRFFREKDKGTITAIVHNES
                     EHPLEGELFINITEDGKSINQKLKLEGNKKGFAIQPHGLTSFNWMIEIPQGISTYKVH
                     LTATADKLSDAEERELPILPSRQRLIESAFVTLSGSQSKKLEISLKDDPTRVSESMVL
                     QIDPQLALSILNTIPFLIDYPYECVEQVLNKYVPLGITNEIYKKYPAIGRAVSKIPDR
                     KTPTPAWEKEDPNRLLALMETPWVWESEGRPTIWPIIDLLDPEIVKAQKEITFNKLRS
                     AQLANGAFPWWPGGEPEPYMTLYVLSGLAQARRHGVEVPTDMIQKALEYVNKEIPLKL
                     KPEQRDLSLVSYAAYVVTSYSPKEYSEAKKGHEAAGSWVIFLERHMHSLTPLGKAYLA
                     YTYLHLGNRKKAESVLDLAMDGAREDPVAGVYWTPEKYSWVWYSDDVEKHAFFLRTLQ
                     ELRPEDKRIPGMVQWLLFNRKGTVWKSTKASAAAVYALLDYLDRRGALASNETFKVEW
                     GKNVYSIVVKADDWLDRPIRWQERGFEITPEKSSAQVQKEGPGITFASLTWTYSTDQV
                     PEASRPGMLELERKFYRRIKEGDAYHLKPIKSGEEVSVGDQIEVQLKINTRSQFEYMH
                     LKDPKAAGFESETLLSGWRRDPLWFYEEPRSSLTNFFISWLPQGEYILRYRMRPTKPG
                     VYRMGAATLQSMYSPDMTAHSAGFVIKVME"
BASE COUNT         3829 a         2440 c         3019 g         3333 t
ORIGIN      
        1 ctggcaagag agcacaacct cgacccgatt aacatcaaag atatgggaga aatggaggaa
       61 aaggcctggg gagatatttt ctcaaaaggg gtgaatttag ccaatcagga tagctctgag
      121 gaagccctga aatggttcaa attggccata agtattaaac cggaaaagta tgatggttat
      181 ctgaattatt gcgtgactag tgccaagatg gacaaaatca ctgaagcttt gccttattgc
      241 gataaagctt accagcttat gcccgaagat ggaaatgtgc agaacatcta tgcctttgtc
      301 ttggtgaacg ccaagcaata tgataaagct ttaggtatct ataataaaat attagagcgc
      361 gatcccacta acataaatgc cttgaccaac ttggcaatga tttatggtca gaaaggtgac
      421 gacagcaatg caatgcaggt ctacaataag atattgcagg tcgatccgga acaaaaagat
      481 acttatttca atcgtggact agttttcatg aagcaagccc aggcgtatta tgaacaaatg
      541 aagcaagcca atgacgctct ggcagctgac ccccaaaata agactttaga agccaaggtg
      601 aggcagttgt tagccgaaca gaaagagatc taccacaaag cggaactgga tttcgccaaa
      661 gctatggagc ttgagcccaa tgataacgaa gccagaggat atttgggagg atctcttttc
      721 catcaggaaa aatgggatga ggcgttacaa gtatttgaga aacttgtcag cctcgacccg
      781 aataacaaag aggcgtggaa ttatctggct gtgctctata ccaagaaggg aatgaaggaa
      841 aagggagaag aggcgctaaa gaaagcgggc atcactcaat agcttgctcg aggcgactgt
      901 ttttagtcca aaaggcagtg gtaccccact gccttttgat tgtctaaagg acgttaaatg
      961 tatgttgatt tagctttacc cctgcccatt aagaagcttt tcacctacgg agttccggag
     1021 agtctagtcg ggaaggtata tttgggtcaa agggtcttgg ttcagttggg aaaaaggtta
     1081 atgatggggg tagtagtcgg actaaatgtt cctgctccac aaatcaagac caaaaatatc
     1141 ctgcttgcac ttgaaacgta tccctggttc agcaacaccg tgctgggtct aacaaaatgg
     1201 atgtcggagt attactccgc cgattgggga gagtgtctga aattcgcctc acctcaatac
     1261 cccgatattt ccggaaggat atggatagaa aatgtgccag ccgatttgga aatgaaaggc
     1321 ttatcagctg aagagaagga tacattggga cttattaaaa gcaaacagaa aatgaaattg
     1381 aacagattag ttcagagttc aggcatggaa gatatctacc agcatctgca gcacctccaa
     1441 caaaagggat tggtcagatt tggattgagg ccgatcaaaa ctgacccaaa agatccagct
     1501 ttgcccctga gaattcgtgc gaaatcaagc tcaaccgaaa tcagtggtct gggtttgaag
     1561 gatgatgaac gaaagattgt ggatttaata tcacaagaca tagaaaagaa aacatattcc
     1621 actttcttat tgatgggaag agatagaatc acaagaagaa tggttcatct ggaattagcc
     1681 aaaaaagcag tgcaagccca gaagcaagtc ctggtcatat tacctgaaac ccatctctta
     1741 gatgagacag tttccagttg gcagagaacg ttcaatgaaa aaatggcggt tttacatccc
     1801 caaatttcaa ttaaccagaa gatgggcatt tgggaaaaga taaagagtaa agaattttct
     1861 ttggttttgg gcacgcgttc agcagtcttt gcccctttgg atgatttggg gctgattgtg
     1921 gtagaagagg agaatcaccc tttacataag gaagaagcgt ctcctcacta caatgccagg
     1981 gatgtggctg tggtgagggg aagattggag aaagccacag ttattttgtc ctgtagcttc
     2041 cccagcttgg aatcctttta caattgccaa agagggaaat ataggttgtg tgatataagg
     2101 gtggaaagca aagcgaggcc tgctctaaag acggagatca tagacaagaa aaagagtggg
     2161 gctaaagcga tcatctcacc cccagtcctc gatatcctga gaaaggagac caagccggtt
     2221 gttctgcttt tgaatcgtcg tggttcttcc agattagtca tctgcgagga ttgtggcttg
     2281 gtcttcagat gtccggattg tggtataagt ctggccttgc ataaggatga gtctgtgctg
     2341 cgatgccgtt actgcaatta tgaacaaaag ggagcgaatg tctgtcctca gtgtggtggg
     2401 tctgattttt cctatcgagg attagggact caagacctgg aacaattctt aagcgaaagc
     2461 ttaccccaaa tcaaaagcgt aagattggat aaggatgtaa cagataaacc aggaggcgcc
     2521 aagaggatct tgttcgaatt ctatcagggg aaatatcagg tccttctggg tacacagatg
     2581 atctttgggg ggttggactt gtcaaaagta ggtttggtga ttatactttc aatagatgag
     2641 cttttgactt atcctgattt tagagcaagg gaaagaacct ttcattttct ctctcagata
     2701 gtggatcgta tgattgagat gaattctcaa gccaaattgg tcctccagac ttcccgacca
     2761 aaagattggg tcatccgctg tgccctgaga ggcgacttcg attatttcta ccagaaagaa
     2821 atagaacaga gaaaagagct tttctatccc ccattcagcc acttgatctc catctggttt
     2881 tcaggcagaa tccaagagaa ggcaatgaaa ttctccagct cttttgtaca gagcctaaat
     2941 cggataattg agcaagggaa agtcagcaaa accaagatat tgggtccgac tcccatctat
     3001 catgctaaaa gaaaaaagcc gaatcagtgg caacttatca taaagaccac atccccaatg
     3061 aaggtaaaca atttaaccaa ttccatcttg aagcgagatg aattcgcaaa gaataggtca
     3121 gttcgaatta aagtagaagt tgacccgtta agtctggtct aaatatatgc agatttccaa
     3181 actattaaag ccggaactta tcaagttgga gatgaaaact aaggtggaat ttccagctga
     3241 ttcatccgtt caccctcaaa agaggatgat gttcataaaa aatgagattc tcaaagagtt
     3301 agtggattta ttggacaaat cgggcagggt ggttaataag aagaagctgc ttttggattt
     3361 cttcaatcgt gagaaaaaag ccaccaccgg gatcggtcag ggaatagcta ttccccatat
     3421 ccgaagcatg caggcgcggg agttcatcct cgggtttgct cgctccaagt cagggtatga
     3481 attcgattct ctggacaaaa aaccggttca cctttttttc gtaatggccg caccacccta
     3541 cgaagataag ctctacctaa agatttttaa agccttggcg gggatgctgc aatatgatta
     3601 ttttatacag aagcttttga aagtctccac ggaatatgag gtcattcgtg ctatagaaga
     3661 aatggaatga gattacacaa agaagaaaag gagctttgtc gtattaagaa taagtttgcc
     3721 aagagtaact aatttaatac aaacgcatta gtttatgttc aatgaatctc ccctcgcccc
     3781 tcttttccaa agagggggaa aaagaggtct ccccctttga aaaaggggga caccaggggg
     3841 attttaaaat agattgcata ttgctctaat atgtaacaac atttaatgcg tttgccatag
     3901 ttaacttcga aattatagaa aggagaaact ttatgcgtaa aatgacccaa gataacctga
     3961 attccgcttt tgctggagaa agtaaggcac atatgcgtta cctgatcttc tcccaatgtg
     4021 cccaaaagga ggggaaacca aacacctcgc gtctttttaa agccatcgcc tttgccgagc
     4081 aagtgcatgc agccaaccat ttcaaggtcc tgggggaatt gggcacaacc tctcagaatt
     4141 tgggggcggc tactgatggg gaggattttg aaattgagga gatgtatcca gcttatctgg
     4201 aagtagccaa actgcaagac gaaaaaacag cccaaaagag catgaattat gccttgcagg
     4261 cggaaaaaat tcattgcgct atgtatcaaa aagctaagca gtcaatagat gagggcaaag
     4321 acgccagttt aaaagatatc tatatttgtg gcgtgtgcgg ctacaccgtg gagggtgaaa
     4381 taccagaggc ctgtccggta tgcaaagcaa ccacagataa attcaagaaa ttttagtaga
     4441 gcccacgagc cctaaccttt aatcctgaag ggggtgaaat agatgccggc caaaaagaga
     4501 aaaaccgccc gtaaggcaaa atccgcctat gtctgtgttc cctgcggggc ggagattaca
     4561 gttaccaaag agggaatggg gacaagtacc ctgatgtgtt gcggccaggt gatgaagagc
     4621 aaggccaaga aaagataacc agtctgggga cacatttcaa gagggtgacc gagaatgtca
     4681 ttgcgaggag cttccgatga aatcggaacg acgaagtccc gccattggcg ggaccacgcc
     4741 ttcggctcgc aatgacagaa atgacgaaat agagggttct cggtcagcct atcaagaccc
     4801 atctcaacac ctgaaaattc ggggaattcg gggaagtctg gacgggagag ttgttttccc
     4861 gtccagactt aaattcaaag ccatcatatc atttaaagtt tgctgttgaa aggccaaacc
     4921 aatggatagt tgcgttttca atgaggtgat tgtttttgcc gcaagaaagg aagcggagtc
     4981 agccaatctt tatcaggttt atgctaatct ggctagcgct tccaatgtca aaaatatgtt
     5041 taaggagatg agaagggaag agctgaagca tcagaaacta ttagagaata tcaaattgaa
     5101 agatatctcc gcgtataagc taaaggatat tcccgacttg aaaatcggtg attattccac
     5161 tcaaggggaa ttcagcccgg atatgaaata tgcggaggcc ttaatgttgg cgattaaaaa
     5221 agaagagatg tcatataaac tgtatcagga tttagctttt agctctaaag atgaagacct
     5281 taaaaagctt ttcttggttt tggtttccga ggaagccaaa cataagttga agttggagga
     5341 agagtacgac aaaacggtta caagcgagaa ctagagatga gaaagagcaa ggagtttctg
     5401 aatatcttgt taggtgcgat ggcgcgtgag accgaagcgt tcaactatta caatgaagcc
     5461 agccagagtt ctccctacga cgaaaccaaa gcgcttttag tacagctctc tctcgaagag
     5521 aagagacatc gggagatatt ggctggagaa tatggaagaa taaagaggct gattttctca
     5581 aaaggaaaag aatatataaa aagggaaaag attagttttg ctattcccaa tgagcttccc
     5641 tatcttaggg tacgcaccat acctggcatt aaggcggcgg caatcgattt gccctctcgc
     5701 tttttgggtg gtgattatgt tgatactttt ccgatcactt tcggcaagaa aagtcttttg
     5761 ggcgtggtca tctacgatgt tatgggtcat ggcatagatg ctactggctt gaaagcggta
     5821 actcgaaaga ctctggatag aatcagggaa gcccttagag aggaagaaat actcaatctg
     5881 gctaatccgg cttggttggt ttctcaagtt aacagtgaat tgtggaggga gtgtaggcag
     5941 aggggttcgt ttgtttcgct gttatatatg gtgattgacc gaataggaag aaaactaacc
     6001 tatacttcag ccgggcacca gccagccgta cttgtaagaa gaaagaatgg agattccgaa
     6061 actctgtcag attcggagct cttggtcgga gtggaagggg aaagagaata tactcaaagc
     6121 agcttagatt tctctcgggg ggatttggtt ctgcttttta cagatggagt ggttgagact
     6181 ctgaatacaa agcaggagga atacggcaca aagcgtctgg ttgaattgtt gaagaaaaat
     6241 agccaattgg atttggctga gcttctattg gagatatgcc aggatttgaa aaaattcaat
     6301 cggggaaaga atttcaaaga tgaggtgagc atgatcgctc tgaaaataga aaaggagaaa
     6361 aataaatgaa gaaagataag gaaaatcttc ccagcgaagc cagaggtcaa gtaccggaaa
     6421 atatgactcc tttggagatt ctgaaattag ctatatatga tgaacaatct gcttgtgact
     6481 tctactccta tctgtctggt cagattagaa accaaagtgg aaaggagaaa ttcaaatttc
     6541 tagcagaaga cgagaaaaga cacaggagca tattagagaa agaatatcaa aaagaaagca
     6601 agggaaggaa gtttgggttt gatcccaaga aggcaaaaac tataagagtc aaagtggacg
     6661 gtgagtccag cgccagcagt gctctggatt tagctatcca ggcggaaaga ggagcttaca
     6721 aattctatac cagtgcagcg gagaaggtga agagtcaatc cagcaagagc atgtttttga
     6781 gattggctga agaggaggac aggcattaca atcttctggc agcggagcgt caagccttga
     6841 gcagtaattt ttactggtat ggatatgacg ttccaggagt aatggaggaa taaaacaagg
     6901 aggagatatg gataagtggc aatgtcaggt gtgtggctat atctatgacc cacacaaggg
     6961 agacccggaa aacggaatcg aggtaggaac atcattcgag agattaccgg atgagtgggt
     7021 ctgtccggag tgcggagcgg ctaaggacca gttcgagaaa atatagagtt tgagttcgct
     7081 taacagaagg aggtacaaca gtggaagccc caagggttaa ggagggggtg tggtgggtgg
     7141 gagcagagga taaagactta cgaatttttg atatcgtaat cccaactgcc tacggtacaa
     7201 cttataactc ctatctgata aagggagaaa agatagcttt ggtggaggga gtgaagaagg
     7261 ggtttcagga gcagcttttc tctcacataa aaagagtttg caatcttgag gatataagat
     7321 acttaatcat caaccacact gagccggatc actccggctg tattgcggag ttaatcgatt
     7381 tggttccaaa tataactttg gtctactcta aaacagccaa gacctttgtg gaaaacatca
     7441 tccatcggga agcagattcg attgtagttg aagatgacga agaactcgac ctgggaggta
     7501 agaccctgaa attcatctct gctccatttc tgcattggcc tgatactatg ttcacctatc
     7561 tggtggaaga cgagattcta ttcccctgtg attgttttgg agcgcattat tgcggggaga
     7621 atatttttaa cgaccagtta aaatcacctc agagcgcttt tggctctttt aagcattatt
     7681 atgaccacat ctttcgtcct ttcaagagtt atgtgctaag agctttggag aaaatagaac
     7741 atctgaagat agatatcata gctccttcgc acggacccat actcaggacc gatccaaaaa
     7801 gttatctgga gtggtacaag agatggtccg aatttccgac cagtgacgat aaaaaaagaa
     7861 taaccattgt ctacgttagc gcctatggca acacccagag aatggcaaaa catattgcca
     7921 atggattaaa gcaaaaaggg gtagaggtat ctttattgga tgccacgcag atagatatgg
     7981 atactatcat caataagata gaggcttcag atggaatcat ctttggcacc cccactttga
     8041 atgccaaagc acccaagccg attctggatg tggtttcaaa tttagtcttg ctcaacattc
     8101 ggggtaagtc tgctgctgtt tttggaagtt atggctggag tggtgaggcg gttgggatgg
     8161 tggaagagat atcgaaaagc atcggattca gaatcaggga gccaggttta aaagttaaat
     8221 tggtccctac ggctcaagat gataagattt gcgaggagtt tgggagcaag tttgcggaaa
     8281 gcttaacggc aaagtgatga tttttcgatc ctgaaacaaa gaagcagggg cttgatttat
     8341 aagttcttgc ctcttataat ctagaaatct ccccttgccc tcgcctcgat tcggcccgcc
     8401 tcggcgagac gggcgaagca ggcctctttt gcaaagaggg gaaaatatat ggataagaga
     8461 tttttcctaa gattcccctt tgagaaaggg ggattaaggg ggattttaga agagatgctg
     8521 aaatacaata agaatctaaa atcactttcc cgagaactaa gaaataatat gacagattcc
     8581 gaaaaactac tttggggtaa gattagaaga aaacaactca aaagttatca gttctatagg
     8641 caaagaacca taggcgacta tattgttgat ttctattgtc ctaaagcagg cttgatcata
     8701 gaagtagacg gagggcaaca ttatagcgac aagggcaaaa ggaaagataa aataagagat
     8761 gtctgtatgg aaagccttgg aatgagggtt ttaaggtttt ctgataagaa gatatttggg
     8821 aatctcgaag gagttctaaa agaaataggg agttatctgt aattggaaaa atctccccta
     8881 acccctcttt tgtaaagagg ggaaaataaa aaatcatttc gcttgaaaat ctctctttgg
     8941 aaaaggggat ttgaagaagt caccaagctc ttatttggta gtgaagtaat aaatatggga
     9001 aaatagactc ctcagtagga acagaagatg gttcggctct acttgattta gcagaaggtt
     9061 actgcaggca acgttccaga tggtgagcat gtcgaaccac agtcccaaca gttcgtttca
     9121 gcgtcaggag tagctcctga cgcagcacag aaatatgtaa gtaggaatat taggtaggga
     9181 cagaacattg ttctgtccct gtttctttac atagtggaaa actttggcag gggcgacccg
     9241 gtggggcacc ccctacaatt ttgggtcttt cttaaatatt ggggagtatt ttctataagt
     9301 cattagtgga gaagaagaaa tagaaaagca tcgattatcc gaatttggat cctggcgacc
     9361 aaaggaaaac cttaacatgg ttagtttagg aaagccaatt gatgcaaatt tagagaattt
     9421 attgttgctg gtctgccttt gtaggggagg cgctcgtcgc ctcccgtctt tcgggagccc
     9481 acgcggttcg acaaactcac cgtcctgagc gaagtcgaag gaaggggctc ccttacgaaa
     9541 ttcccacatt aaatacgaaa gaactacaat ttttgggtgt agatagcgaa acaagtaatc
     9601 tcgcaggcga gactcccagt ctctccgtca tgaaatcaga acggttcatg gctccattat
     9661 aacgtgttgt gatataatgt gataggtaag agtgattttg agcgtagaac aacagtttct
     9721 ccgaattgtc ttcttcctaa gataaaaatt attccaagca atagatttgg aaaaaacctc
     9781 tatattgtat tcttctattt ggattatttg tggacactct ttggacacca gtgagggaca
     9841 actcatccct cccatcccca ttaatcttca aattagggtt ccttgcgaga gaaaaactgc
     9901 agagaagata agggcagtat actaattcac catgcgatct cgtgacctaa ctctatcctc
     9961 attccattac tttaataacg aatccggcgc tgtgtgcggt catgtccgga gaatacattg
    10021 attgcaaggt agcagctccc atgcgataaa ccccaggttt tgtgggtctc attctgtagc
    10081 gcaagatgta ttcaccttgt ggaagccagc ttataaagaa attagtaagc gaactcctcg
    10141 gctcctcgta gaaccaaaga ggatcgcgtc tccaaccact taaaagcgtt tcggattcaa
    10201 agccagcagc tttaggatct ttaagatgca tatactcgaa ttgactacgc gtgttaatct
    10261 taagctgtac ctctatttga tcaccaacag aaacttcctc gccggatttt atgggtttta
    10321 gatgataagc atcgccttct tttattcttc tgtagaactt gcgttcaagc tcgagcatgc
    10381 ctggtcgaga tgcctccggg acttgatcgg tcgagtatgt ccaggtaaga ctggcaaaag
    10441 taatacccgg tccttctttt tgaacctggg cgctactttt ctccggagta atttcgaaac
    10501 ctctttcctg ccagcgaatt ggccggtcta gccaatcgtc cgcttttaca actatagagt
    10561 aaacgttctt gccccattct actttgaatg tttcattact ggccaaagcg cctcgtcgat
    10621 ccagataatc aagaagggcg tagacagctg cagctgaagc cttggtagat ttccaaaccg
    10681 tgcctttgcg gttgaaaaga agccattgca ccattcccgg gatacgctta tcttcagggc
    10741 gcaactcttg taaagtgcgc aagaaaaaag catgtttttc aacatcatca gaataccaca
    10801 cccaggagta tttctccggc gtccaataaa ccccagccac aggatcttct cttgccccat
    10861 ccatcgctaa atccagcaca ctctcagctt tcttcctgtt tcccagatga agataggtat
    10921 aggcaagata agctttgccc agtggtgtca gggaatgcat gtgtcgttct aagaatatta
    10981 cccaagaacc tgctgcctca tgtccctttt tagcttcaga atattcctta ggcgaataag
    11041 aggtcaccac ataagccgca taggaaacaa gagaaagatc tctttgttca ggtttcaatt
    11101 tcaaaggaat ctctttatta acatactcca aggctttctg tatcatatct gtgggaactt
    11161 ctactccatg acgcctggct tgggctaacc ctgaaagaac ataaagggtc atatagggtt
    11221 caggttctcc tccgggccac cagggaaagg caccatttgc tagctgagcc gatcttagtt
    11281 tattgaaggt tatttctttt tgtgccttga ctatttctgg atcaagtaag tcaattattg
    11341 gccaaatagt tggtcgtcct tctgattccc aaacccaagg tgtctccatt agagcaagaa
    11401 gccgattggg atcctctttt tcccaggctg gggtcggggt cttgcgatct ggaattttgc
    11461 ttactgctct tccaatagca gggtattttt tataaatctc attggttatg cctaaaggca
    11521 catacttatt taatacctgt tcaacgcatt cataaggata gtcaatgagg aatggtatgg
    11581 tgtttagtat gcttaaggca agctgaggat caatctgaag gaccatagat tcactcaccc
    11641 tggtcgggtc atctttaagg gaaatttcca atttctttga ttggcttcct gaaagtgtaa
    11701 cgaatgcgga ttcaatcagt ctttgtcttg aagggagaat gggaagctct ctttcctcag
    11761 catcagagag tttatccgct gttgctgtta ggtggacctt gtaggtgctt atgccttgtg
    11821 gtatctctat catccagttg aaactagtta agccgtgagg ttgtatggca aaaccttttt
    11881 tgttaccttc gagttttagt ttttgattga ttgatttgcc atcttcagtt atattaataa
    11941 acaattcacc ttcaagtgga tgttctgatt cattgtgcac aatagcagtt attgtgccct
    12001 tgtctttttc gcggaaaaac ctcggtagat ctgcccgcac cataagttct tttttagtaa
    12061 ccacctcttc ggtcaaggtg ccttgttttg catccttggt gaaagcaagt acttttatcc
    12121 tccagctggt gagttgttca ggagagacaa aagaaaatcg tccgttaccc aatatattcg
    12181 taacaatatg cggcttgaaa aaagctgtat cggcaaattt ccttcttgtt tttattcctg
    12241 cgccagcttc ttggtgttca aattcggatt tatctttttt ctccgaaaca gaggatatcg
    12301 catccgcagc aggggcatat ccattgtacg caaaacctac ctgttctttc agtagttttc
    12361 caccccctcc tccacgataa tctccatttg cccaggtctc ccatgtgcga agtcccggaa
    12421 gtttaggctc gcctgatggc tgacgaaaag ctttaagtag tttctcaagt aaaccctctg
    12481 taatgggtag actatagaca ttagggttaa atacagaata ttgccaataa attggtgtag
    12541 ttcgcaaagc ataaaggtcg tccaaccaga gattgtcgga ctttgcataa tattcaagag
    12601 aacggtcgta catcagtgca a
//
LOCUS       DASWLN010000003         1573 bp    DNA     linear   ENV 20-FEB-2024
DEFINITION  MAG TPA_asm: Candidatus Zixiibacteriota bacterium isolate
            SMAG_U2949 k141_259394, whole genome shotgun sequence.
ACCESSION   DASWLN010000003 DASWLN010000000
VERSION     DASWLN010000003.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37960594
            Sequence Read Archive: SRR10376091
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Candidatus Zixiibacteriota bacterium (soil metagenome)
  ORGANISM  Candidatus Zixiibacteriota bacterium
            Bacteria.
REFERENCE   1  (bases 1 to 1573)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 1573)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (26-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/12/2024 08:28:26
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 1,440
            CDSs (total)                      :: 1,416
            Genes (coding)                    :: 1,412
            CDSs (with protein)               :: 1,412
            Genes (RNA)                       :: 24
            rRNAs                             :: 1 (16S)
            partial rRNAs                     :: 1 (16S)
            tRNAs                             :: 21
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 4
            CDSs (without protein)            :: 4
            Pseudo Genes (ambiguous residues) :: 0 of 4
            Pseudo Genes (frameshifted)       :: 0 of 4
            Pseudo Genes (incomplete)         :: 3 of 4
            Pseudo Genes (internal stop)      :: 1 of 4
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..1573
                     /organism="Candidatus Zixiibacteriota bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_259394"
                     /isolate="SMAG_U2949"
                     /isolation_source="soil"
                     /db_xref="taxon:2053527"
                     /environmental_sample
                     /country="USA: Oregon"
                     /lat_lon="42.99 N 123.63 W"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            complement(<1..1331)
                     /locus_tag="VGB16_00075"
     CDS             complement(<1..1331)
                     /locus_tag="VGB16_00075"
                     /inference="COORDINATES: protein motif:HMM:NF012298.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004672 - protein kinase activity
                     [Evidence IEA];
                     GO_process: GO:0006468 - protein phosphorylation [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="protein kinase"
                     /protein_id="HEX9916111.1"
                     /translation="MENVKMTISTAIESIRKIGEYELLEKIGEGGIAEIFKGIQPSLK
                     REVAIKILSKKLSDDPDVVKRFDQESLIVAGLRHPNIIHVIDKGKKDDNYYFVMEYVD
                     GTDFETILSRGQYNLEQKMDVVIQLCKALDYAHKNNIIHRDVKPANILIDKGGNVLVA
                     DFGIAQMIAKDKRGISEDGWIVGTPSYMSPEQKSGSPNIDLRTDIYAVGVILYEILTG
                     KKPKGRYIPPSELVAEMPKELDNIVVTCLQPDPKDRFKSAVELKNRLLETLSVSKKPK
                     TEEPALVPGVEDLIGKCVFLDTIKQHPYGATYLVKNVETKQLVVIKRLINRVGGLKEA
                     KILCGLRFKHILNIYGAGESKHQLIFVMDYALGGCLGDRMLRQYLWEKALKIVKQAAR
                     GLYHAHNNNIIHGNLRPSNILLDEKDVVKLSDFGLPEHYSEKGVNWYGSPE"
BASE COUNT          394 a          373 c          296 g          510 t
ORIGIN      
        1 ttttctggcg acccatacca gttgacccct ttctcactat aatgttctgg caagccgaaa
       61 tctgaaagct taaccacatc cttttcatcc aagaggatat ttgatggcct caagttcccg
      121 tgaattatgt tattattgtg agcatggtat aaaccacgag ccgcttgctt aacaatcttt
      181 aaagcttttt cccaaagata ttgtctcagc atcctgtcac ccaagcatcc gcccaaggca
      241 taatccataa caaagatcag ctggtgtttg ctctctcctg caccatagat attgagaata
      301 tgcttgaatc taagaccaca gaggatttta gcctctttca gtccaccgac tcggttgatg
      361 agtcttttta ttaccactag ctgcttggtt tcaacatttt taactagata tgtcgcaccg
      421 taaggatgct gttttattgt atccaggaaa acgcacttgc cgattaaatc ctcaactccg
      481 ggaacaaggg ccggctcttc cgttttgggc ttctttgata cggatagagt ctccagaagt
      541 ctattcttca attcaaccgc acttttaaat cggtcttttg gatcaggctg taagcaggtg
      601 actacaatat tatccagctc cttcggcatt tctgcaacta actcggacgg gggtatatat
      661 ctgccttttg gtttctttcc agttagaatc tcatacagaa tcactcccac cgcataaatg
      721 tcagtcctca ggtcaatatt gggcgagcca cttttttgct ccggggacat ataggatggt
      781 gtgcccacaa tccagccgtc ttctgatata cctcttttgt ccttggcaat catctgggca
      841 atgccaaaat ctgccaccaa gacatttccc cctttgtcaa tcaggatatt ggctggtttg
      901 acgtcccggt gaatgatatt gttcttatga gcataatcta aagctttgca aagttgaatg
      961 actacgtcca tcttctgctc gaggttgtat tgtcctcgtg aaagaatggt ctcaaaatct
     1021 gttccatcca catattccat cacaaagtaa tagttgtcat ctttctttcc tttgtctatc
     1081 acatggatta tattgggatg gcgtaagccg gcaacaatta aagattcctg gtcgaaccgc
     1141 ttcaccacat ccgggtcatc gctaagtttc ttggaaagta tctttatggc gacttcacgc
     1201 ttgagtgaag gttgaatccc tttaaagatt tctgcgattc cgccttcacc aattttttcc
     1261 aaaagctcgt attctccaat ttttctaatt gattctatgg cagtggatat tgtcattttc
     1321 acattctcca tctattctgg tatcgtcaga aaaagataat ttttgaagta gcggagaccc
     1381 ttaggtttcc aaaattggag ggctaaaacc ttccactaca taataaaaat cctatctttt
     1441 tgtaggcgac attggccaat atcgccttct tcttttccaa tcctctccct aaccctctcc
     1501 tttctaagga gagggaatgg gtgaggatgt attcttttta gtttgtcatt cccgcgaagg
     1561 cgggaatcca gtt
//
LOCUS       DASWLN010000004         2939 bp    DNA     linear   ENV 20-FEB-2024
DEFINITION  MAG TPA_asm: Candidatus Zixiibacteriota bacterium isolate
            SMAG_U2949 k141_896460, whole genome shotgun sequence.
ACCESSION   DASWLN010000004 DASWLN010000000
VERSION     DASWLN010000004.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37960594
            Sequence Read Archive: SRR10376091
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Candidatus Zixiibacteriota bacterium (soil metagenome)
  ORGANISM  Candidatus Zixiibacteriota bacterium
            Bacteria.
REFERENCE   1  (bases 1 to 2939)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 2939)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (26-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/12/2024 08:28:26
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 1,440
            CDSs (total)                      :: 1,416
            Genes (coding)                    :: 1,412
            CDSs (with protein)               :: 1,412
            Genes (RNA)                       :: 24
            rRNAs                             :: 1 (16S)
            partial rRNAs                     :: 1 (16S)
            tRNAs                             :: 21
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 4
            CDSs (without protein)            :: 4
            Pseudo Genes (ambiguous residues) :: 0 of 4
            Pseudo Genes (frameshifted)       :: 0 of 4
            Pseudo Genes (incomplete)         :: 3 of 4
            Pseudo Genes (internal stop)      :: 1 of 4
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2939
                     /organism="Candidatus Zixiibacteriota bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_896460"
                     /isolate="SMAG_U2949"
                     /isolation_source="soil"
                     /db_xref="taxon:2053527"
                     /environmental_sample
                     /country="USA: Oregon"
                     /lat_lon="42.99 N 123.63 W"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            complement(270..494)
                     /locus_tag="VGB16_00080"
     CDS             complement(270..494)
                     /locus_tag="VGB16_00080"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEX9916112.1"
                     /translation="MKKKILILLVVVLALVFVGQIFAPAPVNARDGSICFKEWYSCPG
                     PRCGQYVEICCIYINHVLRCKAMTVPNLVN"
     gene            complement(887..1105)
                     /locus_tag="VGB16_00085"
     CDS             complement(887..1105)
                     /locus_tag="VGB16_00085"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEX9916113.1"
                     /translation="MKKKILILLLVVLALTFCVQAFTTPSADAKPGYGCFKEWYFCPN
                     HYRWEYVWVCCMLNDRGPCWLKDVAQQL"
     gene            complement(1474..1746)
                     /locus_tag="VGB16_00090"
     CDS             complement(1474..1746)
                     /locus_tag="VGB16_00090"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEX9916114.1"
                     /translation="MKRKTLMVILSISTLLFVFQLHIASAVDKPKPAGPNCGQWAANN
                     LDTSNACQGYTGVHGYDISANGCCITYYCGDRYSYTKCKWDAQAVI"
     gene            complement(2076..2741)
                     /locus_tag="VGB16_00095"
     CDS             complement(2076..2741)
                     /locus_tag="VGB16_00095"
                     /EC_number="3.4.-.-"
                     /inference="COORDINATES: protein motif:HMM:NF014566.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0016020 - membrane [Evidence IEA];
                     GO_function: GO:0004222 - metalloendopeptidase activity
                     [Evidence IEA];
                     GO_process: GO:0071586 - CAAX-box protein processing
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="CPBP family intramembrane glutamic
                     endopeptidase"
                     /protein_id="HEX9916115.1"
                     /translation="MKNKLLTILLLVFLLIIWPLLSYLVMNSSQAELKTQITSRAMQI
                     YLPTILIELLVLLLIILVLRRGKENLKNIGFVGFNLKNLLIGIGFLGLAYLFLSGVSF
                     LLQLYTPKSQENISFLMPHTIIDKSLWIMMSLVAGICEEAAFRGFVLTKLNFWVKRWW
                     PTALISSVFFGLGHLYQGFTGFVLTGIYGFLFCLLFIWRKSLFPGIVAHSLQDIAAIL
                     ISI"
BASE COUNT          926 a          588 c          582 g          843 t
ORIGIN      
        1 ttcggattac accgtttttg ccaaattagg ggattgctat ttcaatctag gtgctttaca
       61 gtcagccagt ttgggataca ggactgcttt gaagttgaat ccgaactttt ctctagcacg
      121 cgataggtta ctagagattg aagaatattt gaaaaagtaa ttctcaatcc agagaaaaaa
      181 taataaagca aaaatgcccc attttgttct ggggcatttt tggtgatttg aacttacctc
      241 tatagttgca attaggcaac tatttggaac tagttaacta aattaggaac tgtcattgcc
      301 ttgcatcgca gaacatggtt tatgtaaatg cagcaaatct cgacatactg cccgcatctg
      361 ggaccaggac aactgtacca ttccttgaag caaatgctac cgtctcttgc attgactggt
      421 gcaggtgcaa aaatctgacc aacgaaaacc aaagccaaaa ctacgaccag aagaataaga
      481 atcttcttct tcatctgtct cacccccttc ttttgtttta agtgagcatt tattctagtt
      541 tatagttata gcaaaatctt tgccagtttg attcgagaaa actattttaa aaaaaccaca
      601 taagtttaat attgacaatt gattgcagag ctgttcagta gttggatttt gaacagacag
      661 aaatttcgtg cttcaattct gcaagagatt gcagattata tgacttatcc tgcttgttga
      721 ttgaagttcc tttgcggagg gatgcccgct gcccgtggtc ggtaaaaaag ccaagaaaca
      781 cgtagccgtc aggtcacagt gaaaaatcaa aatgccccat tttgtctggg acactttgga
      841 taaaactatt tcgtactcac atccgcaatt gcagatagcc cgctatttaa agctgttgag
      901 caacatcctt aagccaacat gggccacggt cgttcagcat acagcagacc caaacatact
      961 cccaacgata atgattaggg cagaaatacc actctttgaa gcaaccataa ccgggctttg
     1021 catcggcgct tggagttgtg aaagcctgca cacagaaagt caaagctaaa actaccaaca
     1081 gaagaataag aatcttcttt ttcatctgtc tcacctcctc tttgttttga ttttttagtt
     1141 ttcaaaactg aatttcaata tcttttcaat ccagatggat gaactcttaa cttcccggct
     1201 cagataaacg caggtggttc ggttccatca cataccccac aaacccagat gcatgttcta
     1261 catgtaccac taccgcattg ccaaacgtaa cacacaagtt tacagcacat tccaggtctg
     1321 gcctgagtgg gtgtggtaaa aagctgggca cagaaagtta ggactaagaa tagtaatagg
     1381 ataataagga tttttctttt catctattgt acctcttttt tgtcagtcag gcattgggtt
     1441 caatttcata atctattact ttatttgttt aacttatatt actgcctgtg catcccattt
     1501 acatttagta taactgtacc tatctccgca gtagtaagtt atgcaacagc cgttagcgct
     1561 aatatcataa ccgtgaactc ccgtataccc ttgacaagca ttggaggtat ctaaattgtt
     1621 agccgcccat tgaccgcaat tcgggccagc tggttttggt ttatccactg ctgaggcaat
     1681 atgaagctgg aacacaaaaa gtaaagttga gatcgataag attaccatta aggtttttct
     1741 tttcatctat ctcacctcta ttcttttgct ttaagtggag attcagtcta attcaattca
     1801 tagaaagcaa aatctctgcc aagtgggatt ggaataatcg acttggcttg atatctataa
     1861 gttgcttgtc gttaatgact tgcgagatgt tcagaattta atcattcctt aaagacggag
     1921 attttagaca tcacgaacgc agaaagttgc gggatatgtg aataggttta cgttatgatt
     1981 agattaatat attgttatca aatgacttag acaatccgcc atttttggcg gataagagaa
     2041 taattttgcg ggtattatat acttgtttaa gaaaattaga tagagataag aatggcggct
     2101 atatcctgca aggagtgggc gactattccc ggaaataggg attttctcca gatgaaaaga
     2161 agacagaaca aaaacccata aattccagtt agtacaaaac cagtgaagcc ttgataaaga
     2221 tgccctaaac caaaaaacac agatgaaatt agggcagttg gccaccacct tttaacccag
     2281 aagttaagct tggtcaaaac gaaacccctg aaagcagctt cctcacatat tcccgctacc
     2341 aaggacatca ttatccagag ggatttatca attatggtat gaggcataag gaaggagata
     2401 ttctcctgcg atttaggcgt gtagagttgt aaaagaaagg agacgcccga aagaaaaaga
     2461 taagccaagc ccaaaaagcc gatgccaatt aataggtttt tcaaattgaa tccgacgaaa
     2521 cctatgttct tgagattctc ctttcctcgg cgcaggacaa gaattatcaa aagcaaaacc
     2581 aaaagctcaa tcaaaatcgt gggaagataa atctgcatag ctcggcttgt gatttgagtt
     2641 ttcagttcag cttgacttga gttcataacc aggtaagata agaggggcca aataatcaag
     2701 agaaaaacaa gaagaaggat ggtcaaaagt ttgtttttca taataaaaga aaagacttct
     2761 gttcgggtgg gacgagtttc ctgcccttac agaaacatgt caggagtagc tcctgacata
     2821 acccagaaat ctcaccctta acagtttttc aaaagtctat aggtctaaaa acctgtaggg
     2881 gctcgattca tggttcgact gggtcccttc gaccttgctc agggacagtg agcgaagtc
//
LOCUS       DASWLN010000005         5311 bp    DNA     linear   ENV 20-FEB-2024
DEFINITION  MAG TPA_asm: Candidatus Zixiibacteriota bacterium isolate
            SMAG_U2949 k141_1147272, whole genome shotgun sequence.
ACCESSION   DASWLN010000005 DASWLN010000000
VERSION     DASWLN010000005.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37960594
            Sequence Read Archive: SRR10376091
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Candidatus Zixiibacteriota bacterium (soil metagenome)
  ORGANISM  Candidatus Zixiibacteriota bacterium
            Bacteria.
REFERENCE   1  (bases 1 to 5311)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 5311)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (26-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/12/2024 08:28:26
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 1,440
            CDSs (total)                      :: 1,416
            Genes (coding)                    :: 1,412
            CDSs (with protein)               :: 1,412
            Genes (RNA)                       :: 24
            rRNAs                             :: 1 (16S)
            partial rRNAs                     :: 1 (16S)
            tRNAs                             :: 21
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 4
            CDSs (without protein)            :: 4
            Pseudo Genes (ambiguous residues) :: 0 of 4
            Pseudo Genes (frameshifted)       :: 0 of 4
            Pseudo Genes (incomplete)         :: 3 of 4
            Pseudo Genes (internal stop)      :: 1 of 4
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..5311
                     /organism="Candidatus Zixiibacteriota bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_1147272"
                     /isolate="SMAG_U2949"
                     /isolation_source="soil"
                     /db_xref="taxon:2053527"
                     /environmental_sample
                     /country="USA: Oregon"
                     /lat_lon="42.99 N 123.63 W"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            complement(296..1660)
                     /gene="hslU"
                     /locus_tag="VGB16_00100"
     CDS             complement(296..1660)
                     /gene="hslU"
                     /locus_tag="VGB16_00100"
                     /EC_number="3.4.25.2"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011417326.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_function: GO:0008233 - peptidase activity [Evidence
                     IEA];
                     GO_function: GO:0016887 - ATP hydrolysis activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="ATP-dependent protease ATPase subunit HslU"
                     /protein_id="HEX9916116.1"
                     /translation="MNANELTPKQIVQELDKYIIGQDKAKKSVAIALRNRWRRQQVSE
                     DLRDEILPNNIIMIGPTGVGKTEIARRLAGLAGAPFIKVEASKYTEVGYVGRDVESMV
                     RDLVDLAVNMVKKEKSEAVSQKVEVLAEERILDLLLPIPKKLPVKPGELVGMEEETEE
                     KRERTREKLREQLRSGKLNQRYVEVKTAEERFPVVEIFSPTGVEELGINIQEMFSGMM
                     PHKTRRRKVTVEEAQRILTQEEINKLVDVEEVMSESIKRVENSGIIFVDEIDKIAGEK
                     AKGQIDVSREGVQRDILPIVEGTNVMTKYGMVRTQHVLFIAAGAFHISKPSDLIPELQ
                     GRFPIRVELDSLSTKDFIRILTEPQNALIKQYTALLATEGVELSFSKEAIEEIAKIAN
                     LVNQRMENIGARRLHTVMTALLEDIMFEASELKGKKVKVTPAMVKDTLKSIVEDEDLS
                     RYIL"
     gene            complement(1661..2194)
                     /gene="hslV"
                     /locus_tag="VGB16_00105"
     CDS             complement(1661..2194)
                     /gene="hslV"
                     /locus_tag="VGB16_00105"
                     /EC_number="3.4.25.2"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_008930782.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004298 - threonine-type endopeptidase
                     activity [Evidence IEA];
                     GO_process: GO:0051603 - proteolysis involved in protein
                     catabolic process [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="ATP-dependent protease subunit HslV"
                     /protein_id="HEX9916117.1"
                     /translation="MQNIRSTTILGLIHKGQVALGGDGQVTVDNAIMKQKAKKLRKLY
                     KNTILAGFAGGAADALTLFERFEGKIEKYHGNLPKAAVELAKDWRMDKYLRRLEALLA
                     VLDKEHGLIISGNGDVIEPDDGIVAIGSGGAYALAAARALKTYSKLDAKSIVEKSIEI
                     ASSICVYTNNQIMVEEL"
     gene            complement(2200..3120)
                     /gene="xerC"
                     /locus_tag="VGB16_00110"
     CDS             complement(2200..3120)
                     /gene="xerC"
                     /locus_tag="VGB16_00110"
                     /inference="COORDINATES: protein motif:HMM:NF001399.0"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0009009 - site-specific recombinase
                     activity [Evidence IEA];
                     GO_process: GO:0006310 - DNA recombination [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="tyrosine recombinase XerC"
                     /protein_id="HEX9916118.1"
                     /translation="MQDLEKQFQLFLAELDGKDYSTHTVSSYRLDLEDFSRFLESEKK
                     DTNSPFSDIDLKLLRNYLIWLKRRRITHRSIRRKISSLSSFFKFLERKGKLKENVVKQ
                     LTLPRSKQELPRFLSEEQMRKILDSSFDETLWGKRDKAILEVFYSSGIRLSELKGLGV
                     SDIDFRGECIRVLGKGKKERVVPIGQRAIQSLREYLDERKKWLRDKDIKEQDTVFVNK
                     FGDRLSSRGIERLVKKHLLKISEDKKVSTHTLRHTFATHLLDHEADLMAVKELLGHKD
                     LSTTQIYTHVTLQHLKKVYQKAHPRAEKER"
     gene            complement(3491..>5311)
                     /gene="topA"
                     /locus_tag="VGB16_00115"
     CDS             complement(3491..>5311)
                     /gene="topA"
                     /locus_tag="VGB16_00115"
                     /EC_number="5.6.2.1"
                     /inference="COORDINATES: protein motif:HMM:TIGR01051.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0005737 - cytoplasm [Evidence IEA];
                     GO_function: GO:0003677 - DNA binding [Evidence IEA];
                     GO_function: GO:0003917 - DNA topoisomerase type I (single
                     strand cut, ATP-independent) activity [Evidence IEA];
                     GO_process: GO:0006260 - DNA replication [Evidence IEA];
                     GO_process: GO:0006265 - DNA topological change [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="type I DNA topoisomerase"
                     /protein_id="HEX9916119.1"
                     /translation="VQSVALRMICEREEEIEKFVPQEYWSLVAWLETKRKESFPAKLV
                     KINDAEAKIGSQKEVEKIIQDLERQKFRVADIRKEVRKRHPYPPYITSTLQQDAARRL
                     NFSSYRTMKIAQQLYEGVELGEVGNVGLITYMRTDSVRVADIAKEEAKNFIKDNFGSK
                     YVPSITPSYKSRKGAQEAHEAIRPTYVEYSPDKIVRYLSKEQYRLYELIWNRFIASQM
                     RSAEFSLTSVDINAGSHLFRATSSEVLFDGFLRVYREIKEENDEEEEEFKLPELASGE
                     LLELSKLDPKQHFTKPAPRFTEASLIKELEANGIGRPSTYAQILTTVRQRKYVDLDGK
                     RIFPTELGKMIKKILVDNFPDIFEVKFTAKMEEELDKVEEGKDDWVKVLNDFYVPFQR
                     VLKEVEKRKEDMKNMTQEETDEVCDKCNSKMIVKWGRHGKFLACSNYPNCKNTKPLNY
                     KENEKIALSEKCEICGSPMEVKFGRFGRFLACSKYPECKSTRPISIGIKCPERNCGGE
                     IVEKRTKKGRIFYGCSNYPKCKFAVWDKPINQVCPTCGSPILVEKIDRSKGSYLKCIR
                     CDYKKEKSPEEAKPWNKEPPPEGGALKPKIQNRDPEPSSG"
BASE COUNT         1232 a         1368 c         1005 g         1706 t
ORIGIN      
        1 gtctccccct ttgaaaaagg gggatttgat ggttcgatat gggcttcgac tgagtccttc
       61 gacggtgagc cgctcaggac ggtcgaaccg ctcagccgaa tgtcctcacc atctcttcga
      121 gcctgagcct tccttcgaca ctgagcctca ggacgaagtg cagagccgaa ggcctgagca
      181 aagtcgaagg gagggggatt ttaaaataga ttgcatattg ttctaatatg taacaacatt
      241 taatgcgttt gctatagttg acagaacact atagcaaaca ataagacagg caacttcaca
      301 gaatgtatct gctcaagtcc tcatcctcca caatactctt caatgtatct ttcaccatag
      361 cgggagttac cttgaccttc ttacctttaa gctctgatgc ttcgaacatt atatcctcta
      421 acaaggcggt cattaccgta tgtaagcgcc gagcgccgat attctccatc ctctggttga
      481 ctaaattggc gatcttggct atctcctcaa tggcttcttt ggaaaaagac aactcaaccc
      541 cttcggttgc caaaagcgcc gtatattgtt ttataagggc gttttgtggc tcggttagaa
      601 tgcgaatgaa atccttggtg cttaagctgt ccagttccac ccttatggga aagcgaccct
      661 gcagttccgg gattaggtca gatggcttgg aaatatgaaa agctcctgcc gcaatgaaaa
      721 gtacatgctg ggttctgacc attccatatt tggtcataac attggtcccc tcaactatgg
      781 gcaggatatc cctctgcact ccttcgcgtg acacgtcgat ctgccccttt gccttctccc
      841 cggctatctt atctatttcg tccacaaaga ttataccaga attctcaact cgcttgatcg
      901 actcagacat cacttcttcc acatccacca gcttatttat ctcctcctga gttaatatcc
      961 tttgtgcctc ctctactgtg acctttcttc tcctggtttt gtggggcatc attccgctga
     1021 acatctcttg aatatttatg cccaactcct cgactccggt tggcgaaaat atctccacta
     1081 ccggaaacct ctcctccgca gttttgactt ccacatatct ttgatttagt ttcccgctgc
     1141 gcaactgctc ccttagtttc tctctggtcc tctctctttt ttcttctgtt tcctcctcca
     1201 ttccgactag ctctcctggc ttgacgggca acttcttggg gataggtaaa agcagatcca
     1261 agattctctc ttcagctaat acctccacct tttggctgac tgcttcggat ttctcctttt
     1321 tcaccatatt caccgctaaa tccaccaggt ctctgaccat cgattccaca tcccggccta
     1381 cgtaaccgac ttcggtatac tttgaagctt ccactttgat gaaaggagcg ccggctaaac
     1441 cggccaatct tctggcgatt tcagttttac ccacaccagt tgggccaatc attattatgt
     1501 tgttgggcag aatctcgtcg cgcaggtcct cactcacctg ctgccttctc caacgattgc
     1561 gcagagcaat agctaccgac ttcttggctt tgtcctgacc gattatgtat ttgtccaatt
     1621 cttgaactat ctgcttggga gtaagctcat ttgcgttcaa ttataactcc tccaccatta
     1681 tttgattgtt agtataaacg cagatggatg aggctatttc aatcgacttt tcaactatcg
     1741 atttcgcatc caatttggaa tacgttttta aggctcgagc tgctgccaga gcataagctc
     1801 cgcctgaccc gatggctact attccatcat ccggctcaat cacgtccccg tttccggaga
     1861 tgattagacc atgttcttta tccaagaccg ccaggagcgc ttccaatctt ctcaaatact
     1921 tatccattct ccaatccttg gctaattcca ctgccgcttt gggaagattg ccgtgatatt
     1981 tttcgatctt cccttcaaat ctctcaaaaa gagttaaggc atcagccgcc ccaccggcaa
     2041 aaccagccag aatcgtattt ttgtatagtt tcctcagttt cttggctttt tgtttcatta
     2101 tggcgttatc aactgtaacc tgtccgtctc cgcctaatgc cacttgtcct ttatgtatca
     2161 aacctaatat ggttgttgaa cgtatgtttt gcataataat cacctctctt tttctgcccg
     2221 gggatgagcc ttttgataga ctttctttaa atgctgaagc gtgacatggg tatagatctg
     2281 agtggtggat aaatccttat gacctaagag ctccttgact gccatcaggt cagcttcatg
     2341 atccagaagg tgagtggcaa aagtgtgacg caaggtatgg gtactcactt tcttgtcttc
     2401 cgatatcttg agcagatgtt tcttgaccaa tctctctatg cctcgacttg aaagtctatc
     2461 cccgaattta tttacgaata ctgtatcttg ttcctttata tctttatctc taagccactt
     2521 ttttctctca tccaaatact ctctcaatga ttgaattgct ctttgtccaa tgggaactac
     2581 tctttccttt ttgcctttgc ccagaactct tatgcattcc cctctgaaat cgatatctga
     2641 cacgccaagt cccttaagct cagataatct tatgccgctg ctgtagaaaa cctccaaaat
     2701 cgccttatcc ctttttcccc acaaagtctc atcaaaagat gagtccagga tttttctcat
     2761 ctgttcctcg ctcaagaaac ggggtagctc ctgcttggac ctgggcagag tcaattgctt
     2821 gaccacattc tccttcagct taccctttct ttcaagaaat ttgaaaaatg aactcaagct
     2881 tgaaatcttc ctgcgtatgg accgatgggt tatccttctc ctcttaagcc agattagata
     2941 attgcgcaaa agcttaaggt caatatcgga aaaaggtgaa ttcgtgtcct ttttttcgct
     3001 ctctaaaaat cgggaaaaat cttcaaggtc gagccggtaa gatgaaacgg tgtgggtgga
     3061 gtaatctttg ccatcaagct cagccaaaaa cagctgaaac tgcttctcta aatcttgcat
     3121 agactattct ttttattatt gagtttcgta aattagattg ataatttttc ttcctctccc
     3181 tttgggggag aggatattga aaacttaaaa attcgttacg aattttaaag ttttcattaa
     3241 aggtgagggg gcgcaaactc tatctcaccc tcatcctaac cttctccctc aagggagaag
     3301 gaaaatgaac tgcttcgtca ctgaattatg cgatttccaa taagatcgca cctgaaaaac
     3361 cccttccttt agacggcgat aagaggtgca tctcagagat gctgaaacga gttcccttcg
     3421 actttgccca gggacggtga gcgtagtcga accgtcagca tgacaaccgg gtctggtttt
     3481 acggtttttg tcatcccgaa cttggttcgg gatctcggtt ttgaattttg ggttttaaag
     3541 ccccgccctc gggcgggggt tctttattcc agggttttgc ttcctctggg gatttctctt
     3601 ttttataatc acatcgaatg cacttaaggt agcttccctt ggacctatcg attttctcaa
     3661 ccaaaatcgg actgccacag gttggacaga cttggtttat cggtttgtcc caaaccgcaa
     3721 acttgcactt gggataatta gaacaaccat aaaatattct tccttttttg gtccttttct
     3781 cgacgatctc tccaccacag tttcgctctg ggcattttat gcctatggaa atcggcctgg
     3841 tgctcttgca ttcaggatat ttggagcaag ccagaaacct tccgaatctt ccaaatttca
     3901 cttccatggg cgagccacag atctcgcact tctcggacag agcgatcttt tcgttttcct
     3961 tataattaag gggcttggtg ttcttgcagt tgggataatt agagcatgct aagaacttgc
     4021 cgtgccttcc ccatttcact atcatcttgc tattgcattt atcacaaacc tcatccgtct
     4081 cttcctgcgt catattcttc atatcttctt ttctcttttc cacttctttg agcacacgtt
     4141 gaaagggaac gtaaaaatcg ttcagcacct tcacccaatc atcctttccc tcttccacct
     4201 tatctaactc ctcttccatc ttagcagtga atttaacctc aaatatatca gggaaattat
     4261 ccactaatat cttctttatc attttgccta attccgtggg aaagattctc tttccgtcca
     4321 ggtctacgta ctttctttgc ctgacggtgg tcaatatctg agcgtaggtg ctcggtcgtc
     4381 cgattccgtt tgcttccagc tcctttatca agctcgcctc ggtgaatctg ggagcaggtt
     4441 tggtgaaatg ctgtttgggg tccagtttag aaagttccaa tagttcgcca ctcgccagct
     4501 cgggtagctt aaactcttct tcttcctcat cattctcttc ctttatttcc ctgtaaaccc
     4561 tgaggaaacc atcaaacagc acctctgagc tggtagccct gaaaaggtga ctgccggcat
     4621 ttatatccac tgaggtcagg ctgaattccg cactgcgcat ctgagaggcg atgaaacgat
     4681 tccagatcag ctcataaagt ctgtactgct ctttacttaa atagcgcact attttgtctg
     4741 gagaatactc cacataggta ggccttatgg cttcgtgtgc ctcctgtgct ccctttcttg
     4801 atttataact cggggttatg gacggaacgt attttgaacc aaaattgtct tttatgaaat
     4861 ttttagcttc ctctttggct atgtccgcta ctctaactga atcagtgcgc atataggtga
     4921 ttagcccaac gtttcccacc tcgcccaatt ccactccctc atacaattgt tgagctatct
     4981 tcatcgtcct gtaagatgag aagttcaatc ttcgagcggc atcctgctgt aaggtactgg
     5041 ttatataggg aggataaggg tgtcttttcc gaacttcctt tcttatatca gctactctaa
     5101 acttctgcct ttctaaatcc tgaattatct tttcaacttc tttctgactg ccgatcttgg
     5161 cttccgcatc atttattttg accagcttag ccgggaagga ttcttttctt ttggtttcca
     5221 accacgcaac cagactccag tattcctgtg ggacgaattt ctctatctcc tcttctctct
     5281 cacaaatcat tctcagcgct actgactgca c
//
LOCUS       DASWLN010000006         6470 bp    DNA     linear   ENV 20-FEB-2024
DEFINITION  MAG TPA_asm: Candidatus Zixiibacteriota bacterium isolate
            SMAG_U2949 k141_8894, whole genome shotgun sequence.
ACCESSION   DASWLN010000006 DASWLN010000000
VERSION     DASWLN010000006.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37960594
            Sequence Read Archive: SRR10376091
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Candidatus Zixiibacteriota bacterium (soil metagenome)
  ORGANISM  Candidatus Zixiibacteriota bacterium
            Bacteria.
REFERENCE   1  (bases 1 to 6470)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 6470)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (26-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/12/2024 08:28:26
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 1,440
            CDSs (total)                      :: 1,416
            Genes (coding)                    :: 1,412
            CDSs (with protein)               :: 1,412
            Genes (RNA)                       :: 24
            rRNAs                             :: 1 (16S)
            partial rRNAs                     :: 1 (16S)
            tRNAs                             :: 21
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 4
            CDSs (without protein)            :: 4
            Pseudo Genes (ambiguous residues) :: 0 of 4
            Pseudo Genes (frameshifted)       :: 0 of 4
            Pseudo Genes (incomplete)         :: 3 of 4
            Pseudo Genes (internal stop)      :: 1 of 4
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..6470
                     /organism="Candidatus Zixiibacteriota bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_8894"
                     /isolate="SMAG_U2949"
                     /isolation_source="soil"
                     /db_xref="taxon:2053527"
                     /environmental_sample
                     /country="USA: Oregon"
                     /lat_lon="42.99 N 123.63 W"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            <1..222
                     /locus_tag="VGB16_00120"
     CDS             <1..222
                     /locus_tag="VGB16_00120"
                     /EC_number="6.4.1.2"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016198617.1"
                     /note="an AccC homodimer forms the biotin carboxylase
                     subunit of the acetyl CoA carboxylase, an enzyme that
                     catalyzes the formation of malonyl-CoA, which in turn
                     controls the rate of fatty acid metabolism; Derived by
                     automated computational analysis using gene prediction
                     method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="acetyl-CoA carboxylase biotin carboxylase
                     subunit"
                     /protein_id="HEX9916120.1"
                     /translation="PFYDSMIAKLITYGKTREEAILKMRRALEEFIIEGVKTTIPFHQ
                     KILSDSQFISGNFDTSFVESVLKQKENQT"
     gene            235..618
                     /gene="gcvH"
                     /locus_tag="VGB16_00125"
     CDS             235..618
                     /gene="gcvH"
                     /locus_tag="VGB16_00125"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_007506409.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003824 - catalytic activity [Evidence
                     IEA];
                     GO_process: GO:0019464 - glycine decarboxylation via
                     glycine cleavage system [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="glycine cleavage system protein GcvH"
                     /protein_id="HEX9916121.1"
                     /translation="MNIPADLKYTKEHEWLKVEGQLATMGITDYAQGELGDIVFTELP
                     KVGTRLEQMKPFGVIEAVKAVSEIFSPITGEVTEVNSKLESEPTTMNSDPYGAGWIIK
                     IKVANHKELNNLLSAEEYKKLLEEH"
     gene            631..2103
                     /locus_tag="VGB16_00130"
     CDS             631..2103
                     /locus_tag="VGB16_00130"
                     /inference="COORDINATES: protein motif:HMM:TIGR04514.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="GWxTD domain-containing protein"
                     /protein_id="HEX9916122.1"
                     /translation="MKRKCCILPLFTILICLLGCSATDRYLRRATPEIRDTYYELRPL
                     LTDEEALEFLKLSRVEREEWLRKFWKSKDPTPTTERNEFKEEHERRVEYTKAKFGSNF
                     TMRPWDDRGEVYIKYGEPDEREPGIYEISDRKGYIGRKNTRCLLAAEDARNRGEVWHY
                     YQYNLSLQFEDYRKIGYLYLVPYISEIEANRVKPLPHEAMDRQQSFYATKAEKIDMQK
                     EIYTHDYEGEPLDYALDVASFRFKKDLYDVDVNIGVPTDKLGQSENDTTAQGGLMRRI
                     SVFDQNLVEVAKDSTTTYYSATSNKGLLLVDQKTFHLTPGEYTIAVEVKDLATNRIGI
                     YKKEIVLPAHTDPKEQYISQIIMAAEIRPPQPNERKYIKHDLAVVPLPSKTYYADQMI
                     KFYYEVYNLKLDNEGEVHYLVNYDLVNTKEKKEENIFNSFVLMAKSADIYEVGYIDPA
                     EISAGDYILVARVQDALSGKIMKSVSPFRVLETKKEGEKR"
     gene            2103..3443
                     /gene="gcvPA"
                     /locus_tag="VGB16_00135"
     CDS             2103..3443
                     /gene="gcvPA"
                     /locus_tag="VGB16_00135"
                     /EC_number="1.4.4.2"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_020250337.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004375 - glycine dehydrogenase
                     (decarboxylating) activity [Evidence IEA];
                     GO_process: GO:0006546 - glycine catabolic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="aminomethyl-transferring glycine dehydrogenase
                     subunit GcvPA"
                     /protein_id="HEX9916123.1"
                     /translation="MPYVPHTNDDEKKMLEKIGVKDFSGLLEPIPQSLRLKNKLNLPE
                     PLSEMELVKLLSQMAEENKNLNRVISFLGAGAYDHFIPSVVNHIISRSEFYTAYTPYQ
                     AEVSQGTLQTIYEFQSLICQLTGMDVANASMYDGASAVAEAALMAHAHTNREVILVSD
                     SINHHYRVVLDTYANALPIEIKTIPLEDGLVDINYLEKRMDEKVAGLIVQSPNLFGLL
                     ENIEKIEPIVHSKGGLLIMVCDPISLGILKSPGDYNVDIALGEGQGMGIPLSFGGPYL
                     GFFAAKQELIRRMPGRIVGETVDSQDRRGYVLTLQTREQHIRREKATSNICTNEALCA
                     LASAVYLSLMGKEGLRKVAELCLQKSHYAFEQICEIKGFKPTFTASFFKEFVIQTPIP
                     PREIIDSLSGKNIFAGVDLNRFSIEMGNALLVCVTEKRTKEEIDYFVSELKRLV"
     gene            3446..3799
                     /locus_tag="VGB16_00140"
     CDS             3446..3799
                     /locus_tag="VGB16_00140"
                     /inference="COORDINATES: protein motif:HMM:NF020967.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF2007 domain-containing protein"
                     /protein_id="HEX9916124.1"
                     /translation="MPFCPKCKYEYKPGIEICPDCDEKLVDSLPEEESDEEPELDEEI
                     ELALLCTTASLVHTNFLKEALKDNDIPCLVKREGPSIYGSGVVGYAFFGSKIYVPRDK
                     LEEAKRIKDQTIDNL"
     gene            3826..4152
                     /locus_tag="VGB16_00145"
     CDS             3826..4152
                     /locus_tag="VGB16_00145"
                     /inference="COORDINATES: protein motif:HMM:NF020967.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF2007 domain-containing protein"
                     /protein_id="HEX9916125.1"
                     /translation="MCKAEYAAGVKRCVGCECDLMEDLSEEEGLDEEGEWVLLYSTTN
                     RVYAEFLKETLEESNIRAIIRSSGSFLEHGLGYVSKGQTGYKIYVQEEYYEESSAIKD
                     QTVGNL"
     gene            4173..5018
                     /locus_tag="VGB16_00150"
     CDS             4173..5018
                     /locus_tag="VGB16_00150"
                     /inference="COORDINATES: protein motif:HMM:NF012314.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="thioredoxin fold domain-containing protein"
                     /protein_id="HEX9916126.1"
                     /translation="MRFFRGVVGLLVAMFVLSGCAKKVPEKIGWVYKLEEGKKLAQKE
                     HKGLIVEFFKDDCPWCKRMDDSTFVAENVRKLNWNYVFVKIDGKAETTLVSQYGITGY
                     PTSIVFSSPDKELDRIIGYLPADSFEVYVRNYEKGIGTLEYYEKKVAADSSNVELIYE
                     LAEKYKWHNRPQEALTYFYKIVALDPPNVKKYSDRAVYEAADLYFRNKDYKGALGEFD
                     NLLEKYPESKYKLDAELMVAYCYAKLDNKTTALGLYKKFLKEHPGKEGEWVQEQIDKL
                     EGKVK"
     gene            5015..>6470
                     /gene="gcvPB"
                     /locus_tag="VGB16_00155"
     CDS             5015..>6470
                     /gene="gcvPB"
                     /locus_tag="VGB16_00155"
                     /EC_number="1.4.4.2"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_012500106.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004375 - glycine dehydrogenase
                     (decarboxylating) activity [Evidence IEA];
                     GO_process: GO:0006546 - glycine catabolic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="aminomethyl-transferring glycine dehydrogenase
                     subunit GcvPB"
                     /protein_id="HEX9916127.1"
                     /translation="MSERLIFELSSKGRKGYSLPELDVPAKKTEELVPLEKLRNQAPP
                     LPELSEVDVVRHFVRLSILNHHVDKGFYPLGSCTMKYNPKVNEDISRLSGFSQIHPFQ
                     PEESVQGTLKLMYQLGEYLKEISGLDGITLQPAAGAHGELTGLLIIHAYHKKAGNPRS
                     KIIIPDSAHGTNPASVITAGYRTVQVKSNPKGLVDLEELKKELDDEVAAVMLTNPNTL
                     GLFESQISEISKIVHSHGALLYMDGANLNAMLGIARPGDMGFDVVHFNLHKSFSTPHG
                     GGGPGSGPVGVKKDLIKFLPVPIVEKLISRRGIREDEEKYHLNYNLPDTIGKIHGFYG
                     NFGVMVKAYSYIRHLGADGLREVSEGAILNANYLKESLKDIYHLPYDFICQHEFVFSG
                     KNQKAKGVKTLDIAKRILDFGMHAPTVYFPLIVSEALMIEPTETESKESLDGFISVMR
                     QIAREVEENPEIVKSAPHNTPVSRLDEVKAARDLN"
BASE COUNT         2019 a         1175 c         1589 g         1687 t
ORIGIN      
        1 cctttttacg attcaatgat agctaaactt ataacttatg gcaaaacaag ggaggaagca
       61 atacttaaga tgaggcgggc attggaggaa tttatcatcg agggagtgaa gaccacaatt
      121 ccctttcatc aaaaaatctt atctgatagc cagttcatca gtggaaattt tgacacctcc
      181 tttgtggaga gcgttttaaa acaaaaggaa aatcaaactt aaaaaggaga tagggtgaat
      241 ataccagcgg acttaaaata caccaaagag catgaatggt taaaagtgga aggacaactt
      301 gccactatgg gcatcactga ttacgctcaa ggggaattgg gtgatatcgt ctttacagag
      361 ttgcccaagg ttggcaccag gctcgaacag atgaaacctt ttggggtaat tgaagcagtt
      421 aaggcagtgt ccgagatttt ctctcctatt actggagaag tgaccgaagt caactccaaa
      481 cttgaatcag agccaaccac catgaactcc gacccatacg gagcggggtg gattatcaaa
      541 atcaaagttg ccaaccacaa ggagttaaat aaccttcttt cagctgaaga gtacaagaaa
      601 cttttggaag agcattaatt ataaatcctc atgaagagaa agtgctgtat tcttcctctc
      661 tttacgatct taatctgcct acttggttgc tccgctacag ataggtactt gagaagagct
      721 actcctgaaa tcagagatac ctattacgag ctcagacctc tcttaacgga tgaggaagca
      781 cttgagtttc taaagctttc tcgtgtagaa agggaggaat ggttaagaaa attctggaaa
      841 tcgaaggatc caactcccac aactgaaaga aatgagttca aagaggaaca cgaaagaaga
      901 gtagaatata ccaaagctaa gttcgggtcg aatttcacta tgcgtccctg ggatgataga
      961 ggtgaggtat atatcaagta tggtgaaccg gatgaaaggg aacccggaat atatgaaata
     1021 agcgatagga aaggttatat aggtcgtaaa aatacaaggt gtttattagc agcagaggac
     1081 gcacgtaata gaggtgaagt ttggcactat taccagtaca atttgagtct tcagttcgaa
     1141 gattatcgta agatagggta tctgtatttg gtcccatata taagtgaaat tgaagccaat
     1201 agagtgaaac cattacccca cgaggctatg gatagacagc agagtttcta tgccactaaa
     1261 gccgagaaaa tagatatgca gaaggaaatc tatacgcacg actacgaagg agagcctttg
     1321 gattatgctc tggatgtagc cagcttcagg ttcaaaaaag acctgtatga cgtggatgtc
     1381 aatattggag ttcctactga taagttagga caaagtgaaa atgatactac tgcacaaggc
     1441 ggcttgatgc gcaggatttc agttttcgat caaaatttag ttgaagtagc taaagatagc
     1501 acgactactt attattccgc cacttccaac aaaggtttgc tcttagttga ccaaaagact
     1561 tttcatttaa ctccggggga atacactatt gcagtggaag tgaaggattt agccacaaat
     1621 cgtattggaa tctacaaaaa agagatagtc ctgccagcac acactgaccc caaggaacag
     1681 tatataagcc agataattat ggcagcggaa attagacctc cccagccaaa cgagaggaaa
     1741 tatatcaagc atgatttagc agtagttcct ttgcccagta agacctatta tgctgaccag
     1801 atgattaagt tttactatga ggtgtacaat ctgaaattag acaacgaagg agaagttcat
     1861 tacttagtga attatgattt ggtcaatacc aaagagaaga aagaagagaa tatatttaac
     1921 tcttttgtct tgatggccaa atctgcggac atctatgagg taggatatat tgacccagcc
     1981 gagatttcag ccggagatta tatcttggtg gcaagagttc aggatgcttt aagtggaaaa
     2041 ataatgaaga gcgtttcacc ttttcgtgta ttggagacca agaaggaagg agaaaaacgg
     2101 taatgcccta cgttccgcat acgaatgatg atgaaaagaa aatgttagag aaaatcggag
     2161 tcaaggattt ttctggactt ttggagccga ttcctcagtc attgcgattg aaaaacaaac
     2221 tcaatttacc tgagccactt tccgagatgg agttggttaa actcttaagt cagatggcag
     2281 aggagaataa gaatttaaat cgggtaatca gtttcctggg cgcaggagct tatgaccatt
     2341 tcatcccttc agtggtaaat catattatat cccgctcgga attttatacc gcctacaccc
     2401 cttatcaagc ggaggtcagc caaggaactt tgcagacgat atatgaattt cagtcgctga
     2461 tttgtcaact gaccggaatg gatgtagcca acgcatcgat gtatgatgga gcttcagctg
     2521 tggctgaagc agcgttgatg gcacatgcgc ataccaatag agaagtcatt ttggtttcgg
     2581 attcaatcaa tcatcattac cgcgtagttt tggatacata cgccaatgcc ctgccaatcg
     2641 agattaagac tattccttta gaagacgggt tagttgacat aaattatttg gaaaagagaa
     2701 tggacgagaa ggtggctggt cttattgtgc aatctcctaa tctttttggc ttactggaaa
     2761 acattgagaa gatagagccg attgttcatt ccaaaggcgg gctcctgata atggtttgtg
     2821 accccatttc cttgggaatc cttaaatccc cgggagatta caatgtggat atcgctttag
     2881 gagaagggca gggaatggga attccacttt cctttggcgg accgtatctc ggattttttg
     2941 cagccaagca ggaactcatc cgtaggatgc ctggaagaat cgttggcgag acagttgaca
     3001 gccaagatag gagaggatat gttttgactt tgcagaccag agagcaacat atacggagag
     3061 aaaaggcaac ttcaaatatc tgcacgaatg aagcactctg cgctttggct tcggcagtat
     3121 atctctctct tatgggaaaa gagggtttaa gaaaggtagc ggagttgtgt ttacagaaaa
     3181 gccattatgc ttttgagcag atttgcgaga ttaaaggatt taagccaact ttcactgctt
     3241 cattttttaa ggaatttgta atccaaaccc ccatccctcc aagagagata atagattcgt
     3301 tgtccggcaa gaatatattc gcaggtgtgg atttgaatcg ctttagtatt gaaatgggaa
     3361 atgccctttt agtctgtgtt accgagaaga gaaccaaaga ggagatagat tattttgtgt
     3421 ccgaactcaa aaggttggtg taaagatgcc attttgtcct aaatgtaaat acgaatacaa
     3481 gccgggaata gaaatttgtc cggattgcga tgaaaaatta gttgatagct tgccggaaga
     3541 agaatcagat gaagagccgg aattagatga ggaaattgaa ttggctttac tttgcactac
     3601 cgccagctta gttcatacta atttcttgaa agaggcatta aaggataatg acatcccttg
     3661 tttggtaaaa agagaaggtc cgagcatata tggctccggc gttgtaggtt atgctttctt
     3721 tggttctaaa atttacgttc ccagggacaa gctggaagaa gccaagagaa taaaggacca
     3781 aaccatcgat aatctttaaa ttctaaatct atgccttttt gtcccttgtg taaagcagaa
     3841 tatgcggctg gggttaaaag atgcgtgggg tgtgagtgcg atttgatgga ggatttgtct
     3901 gaagaggaag gtctggatga ggaaggggaa tgggttctgc tttattcgac aactaatcgg
     3961 gtctatgccg agttcctgaa agaaacattg gaggagtcta atattcgtgc tatcatacgt
     4021 tcgagcggct cctttcttga acacggatta ggttatgtta gcaagggtca aacgggttat
     4081 aagatttatg tgcaggagga gtattacgag gaaagctcgg ctattaaaga ccaaaccgtg
     4141 ggaaacctgt aaacttttta caaggaggag gtatgagatt ctttagagga gtggttggat
     4201 tgttagttgc gatgttcgtg ctttcgggtt gcgccaagaa ggttccggag aaaataggat
     4261 gggtttacaa attggaagag gggaaaaagt tagcacaaaa agagcataag ggtttaatcg
     4321 tcgagttctt taaggatgat tgtccgtggt gtaagcgcat ggacgattcc acctttgtag
     4381 cggaaaatgt aagaaaattg aattggaatt atgtttttgt gaagatagat ggcaaggcgg
     4441 aaacaacctt agttagtcaa tatggaataa ccggatatcc gacttccatc gtcttcagct
     4501 ctccagataa ggagctggac aggattatag gatacctgcc agcagacagt tttgaagtct
     4561 atgtgcgcaa ctacgagaag ggaataggaa ctttggaata ctatgagaaa aaagtggcag
     4621 cggattcttc gaatgttgag cttatctatg aattggcgga gaaatacaag tggcacaaca
     4681 ggccccagga agctctgact tatttctaca aaatagttgc tttagacccg ccaaacgtca
     4741 agaagtactc tgaccgtgcg gtgtatgaag cagcagacct ctatttcaga aataaggatt
     4801 ataaaggagc attgggtgag tttgataatc ttctggaaaa atatcccgaa tcaaaatata
     4861 aattagatgc agaattaatg gtggcttatt gctacgccaa actggacaat aaaacgactg
     4921 ctttggggct ctacaagaaa ttcctaaaag agcatccggg caaagaaggc gaatgggtgc
     4981 aggaacagat agataaactg gaaggaaaag tgaaatgagc gagagattaa tattcgaact
     5041 tagctccaag ggaagaaaag ggtattcact tccggaactc gatgttcccg ccaagaaaac
     5101 agaagagctt gttccattgg agaagctaag aaaccaagcc ccgccacttc ctgagctgag
     5161 cgaggtggat gtggtcaggc atttcgtcag actctcgatt ttgaatcacc acgtggataa
     5221 aggtttttat cctctcggct cctgcaccat gaaatacaat cccaaagtaa atgaggatat
     5281 ctcccgtctt agtggctttt cgcagattca ccctttccaa ccagaggaga gcgtgcaggg
     5341 gactctaaaa ttgatgtatc aattgggaga gtatcttaag gagatatccg gtttagatgg
     5401 gataaccctt cagccagctg ctggggcaca tggggaactg accgggcttt tgataatcca
     5461 tgcctatcac aagaaagctg gcaaccccag gagtaaaatc ataattccgg actcagccca
     5521 tggcaccaat ccagcctcgg tcatcactgc aggatacagg accgttcagg ttaaatctaa
     5581 tccaaaagga ttagtggatt tagaggagct gaagaaagag ttggatgacg aagtggcagc
     5641 agtgatgttg accaatccta atactctggg tctgtttgag tcgcagattt ctgagatatc
     5701 gaagatagtt cattcccacg gggcgttgct ctatatggat ggtgctaact tgaatgctat
     5761 gctgggaatc gctcggcccg gggatatggg attcgacgta gttcatttta atctgcataa
     5821 gagtttttct accccgcatg gtggtggtgg acccggctct ggtccagtcg gagtgaaaaa
     5881 ggatttaata aagttcttac cggttccgat tgtggaaaaa ctaatctcaa gacgcggaat
     5941 tcgtgaagac gaggaaaaat atcatctgaa ttacaattta ccagacacca ttggcaagat
     6001 tcacggcttc tatggaaact ttggagtgat ggtcaaggct tactcttata ttcgtcatct
     6061 gggcgcagac gggttaagag aggtgagcga gggggctatt ctcaacgcta attatctaaa
     6121 ggagagcttg aaggatattt atcaccttcc ttatgacttc atctgccaac acgagtttgt
     6181 gttttctggg aagaatcaga aagccaaagg agtcaagacc ctggacatcg ccaaaagaat
     6241 tctggatttc ggcatgcatg cgccaaccgt ctattttcct ctaattgttt ccgaggcgtt
     6301 gatgatagag ccaactgaaa ctgaaagcaa agagagctta gatggattca tctcagtgat
     6361 gaggcagatc gccagagaag tagaggagaa tccggaaata gtcaaatccg ccccgcataa
     6421 cacgccggtc agtcggttgg acgaggtcaa ggcggcaagg gatttgaacg
//
LOCUS       DASWLN010000007         2979 bp    DNA     linear   ENV 20-FEB-2024
DEFINITION  MAG TPA_asm: Candidatus Zixiibacteriota bacterium isolate
            SMAG_U2949 k141_391304, whole genome shotgun sequence.
ACCESSION   DASWLN010000007 DASWLN010000000
VERSION     DASWLN010000007.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37960594
            Sequence Read Archive: SRR10376091
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Candidatus Zixiibacteriota bacterium (soil metagenome)
  ORGANISM  Candidatus Zixiibacteriota bacterium
            Bacteria.
REFERENCE   1  (bases 1 to 2979)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 2979)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (26-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/12/2024 08:28:26
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 1,440
            CDSs (total)                      :: 1,416
            Genes (coding)                    :: 1,412
            CDSs (with protein)               :: 1,412
            Genes (RNA)                       :: 24
            rRNAs                             :: 1 (16S)
            partial rRNAs                     :: 1 (16S)
            tRNAs                             :: 21
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 4
            CDSs (without protein)            :: 4
            Pseudo Genes (ambiguous residues) :: 0 of 4
            Pseudo Genes (frameshifted)       :: 0 of 4
            Pseudo Genes (incomplete)         :: 3 of 4
            Pseudo Genes (internal stop)      :: 1 of 4
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2979
                     /organism="Candidatus Zixiibacteriota bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_391304"
                     /isolate="SMAG_U2949"
                     /isolation_source="soil"
                     /db_xref="taxon:2053527"
                     /environmental_sample
                     /country="USA: Oregon"
                     /lat_lon="42.99 N 123.63 W"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            146..219
                     /locus_tag="VGB16_00160"
     tRNA            146..219
                     /locus_tag="VGB16_00160"
                     /product="tRNA-Pro"
                     /inference="COORDINATES: profile:tRNAscan-SE:2.0.12"
                     /note="Derived by automated computational analysis using
                     gene prediction method: tRNAscan-SE."
                     /anticodon=(pos:180..182,aa:Pro,seq:cgg)
     gene            444..905
                     /locus_tag="VGB16_00165"
     CDS             444..905
                     /locus_tag="VGB16_00165"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011563504.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="TIGR00725 family protein"
                     /protein_id="HEX9916128.1"
                     /translation="MMNQKIFIGVIGGGFCSKKVYDLALEVGREVAKNDAVLVCGGLG
                     GVMEGAACGAKENGGITIGILPTSKRSDANPYIDYPIPTGLGEARNILVVKSSDVVIS
                     LPGRYGTLSEMAFCLKLRKPLVSLSDWDLAPEIVKAKNPREAVKLALSMVE"
     gene            906..1193
                     /locus_tag="VGB16_00170"
     CDS             906..1193
                     /locus_tag="VGB16_00170"
                     /inference="COORDINATES: protein motif:HMM:NF012911.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="acylphosphatase"
                     /protein_id="HEX9916129.1"
                     /translation="MSDLVCAKIIISGAVQGVGYRYFAVRKANEYGLFGYTRNLYNGD
                     VKVEVEGERGLVSDFIEELKIGPASSSVTNLSIEWKNHQSKYTSFNIEFQP"
     gene            1210..1737
                     /locus_tag="VGB16_00175"
     CDS             1210..1737
                     /locus_tag="VGB16_00175"
                     /EC_number="2.4.2.7"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_007789222.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003999 - adenine
                     phosphoribosyltransferase activity [Evidence IEA];
                     GO_process: GO:0009116 - nucleoside metabolic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="adenine phosphoribosyltransferase"
                     /protein_id="HEX9916130.1"
                     /translation="MTENLKKLIRDVNDYPKKGIIFRDITTLIKNGDAFNQVVDIFCE
                     KCKNEKVDLVAAIESRGFIFGGALAQKLGAGFVPIRKFGKLPADTEVESYDLEYGTDS
                     LEIHKDAVKKGQKVILIDDLLATGGTMQAACKLVEKLGGEVVKILVMIELSFLKGRDR
                     FKNYDYFSLIQYKDE"
     gene            1785..1859
                     /locus_tag="VGB16_00180"
     tRNA            1785..1859
                     /locus_tag="VGB16_00180"
                     /product="tRNA-Arg"
                     /inference="COORDINATES: profile:tRNAscan-SE:2.0.12"
                     /note="Derived by automated computational analysis using
                     gene prediction method: tRNAscan-SE."
                     /anticodon=(pos:1820..1822,aa:Arg,seq:cct)
     gene            2279..>2979
                     /locus_tag="VGB16_00185"
     CDS             2279..>2979
                     /locus_tag="VGB16_00185"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEX9916131.1"
                     /translation="MKLRVSFSLVIFALLFTNLCYAQVIDYNFLGGGARARAMGGAFF
                     AVSDDPSAGSWNPAGLAQLDLPQTNLTLYSGRVKIEHGLGSLSTEAKMSNDLILSGGV
                     AIPFKVSGYQMVGCVSYERLSIFSDQSYYASIDRSEETNGNVNAVTVSLGRELIKGLS
                     LGMAVNIYSGGYTFKAFQADRPFKIGVSDTVWSDTVFSYHPLMEANYSGFNLQFGAMY
                     KIQGLSFGAVVKTPLT"
BASE COUNT          895 a          543 c          741 g          800 t
ORIGIN      
        1 aagctttctg atacaaattt gaactgcggt gtgaatagat ttttgttgat tttgagaaat
       61 agaatgttaa tttagataaa caggcattca ctggagcgag gagagctttc aaaactcaaa
      121 actgaacact taaaactgaa ttaatcgggg cgtggctcag cctggcagag cgcttggttc
      181 gggaccaaga tgtcggaggt tcaaatcctc tcgccccgac atgcaccaga acggtgcatg
      241 tccccgaggt gaaccagaac ggttcacctc cccgaagtaa ttcagttcac agttcacagt
      301 aggtggttca tagtaaaaac atccatagtc tctattatta actgtgaatc aggaagcatc
      361 agccaaaaac agtatttcaa gaaacgagtt ttgagttcta agactcttct cgaaactcaa
      421 aactcgaaac tcaaaactga attatgatga atcaaaaaat ctttattgga gtgataggcg
      481 gtggtttctg ctcaaaaaaa gtatatgacc ttgctcttga agtaggtagg gaagtggcaa
      541 aaaatgatgc agtcttggtc tgtggcggtc tgggtggagt aatggagggt gcggcttgtg
      601 gtgccaagga aaatggtggt ataaccatag ggattttgcc gacgagcaag agaagcgatg
      661 ctaatcctta catcgattac cctattccta ctggcttagg cgaagcaaga aatatactgg
      721 tggtaaaatc atccgacgtg gtcatctccc tgccgggaag atacggcact ctctccgaga
      781 tggctttctg tctaaaactc aggaaacccc tggtcagttt atccgattgg gatttggccc
      841 ctgaaatagt caaagccaaa aatccacgag aggcagtcaa gctggcacta agtatggtag
      901 aatgaatgag tgacttagtc tgcgccaaga taataataag tggagcggtg cagggagtgg
      961 gatatcgcta ttttgctgtg aggaaggcaa acgaatatgg cctctttggc tatacgagaa
     1021 acttatataa tggcgatgtc aaagttgaag tggaagggga gaggggactc gtgagtgact
     1081 tcattgagga actgaaaatc ggaccagctt caagtagcgt cacaaactta agcatcgaat
     1141 ggaagaatca ccagagtaaa tacacgagct ttaacataga attccaacct taactattcg
     1201 gagataaaga tgaccgagaa tttaaaaaaa ctaattcgag atgtgaacga ctatcccaag
     1261 aagggaatta tttttagaga cataactacg ctcataaaaa acggtgatgc atttaatcag
     1321 gtggttgata tcttctgcga aaaatgcaag aatgaaaagg ttgatttggt ggcggctatc
     1381 gaatccagag gttttatctt tggcggcgcg ctggctcaaa aattgggagc tggatttgtg
     1441 cccatacgaa aattcggaaa gctcccggca gatacagaag tggaaagtta tgatttggaa
     1501 tatggaaccg actctttgga aattcataag gacgcagtta agaaaggcca gaaggtgatt
     1561 ttgatagacg accttttagc taccggtgga acgatgcagg cagcatgcaa gttagttgag
     1621 aaattgggtg gtgaggtagt gaagatattg gttatgatag agttgtcctt tctgaaaggt
     1681 agagatcggt tcaaaaatta cgactatttt tctttgatac aatataagga cgaatagact
     1741 ggattgtgct atgaactccg aaccgtgaac tgtgaactga aagtgccccc gtagctcagt
     1801 ttggatagag cagaggtttc ctaaaccttt ggccgcccgt tcaagtcggg ccgggggtat
     1861 gaagacagtt taatagttta ttaagatcgc acctgaaaac ccctctcctg tggacggcaa
     1921 tgaaaggtgc atcttgctgg aagcagaaaa aaacagacca gatgcaaaca aaacaagcct
     1981 gtcctggtat cagaccagag atgctgaaac gagtagatgc tgaaacaagt tcagcatgac
     2041 aacggggttt ggttcccgtc tttgtcatcc cgaacttgat tcgggatctc ggtttgattc
     2101 ggtttggttt tgaattttgg gtttttactt gtagaactgc aatataaaaa gttgcaaata
     2161 gagtcaaatt ctccttgaca agatatttgg aaaggttata taatatgtag ttaaatatat
     2221 gatttgggcg accttaaaac tcgaaactta aaactgaatc aaaaaggagc cgtgtcagat
     2281 gaagctaaga gtttcatttt ccttagtgat ttttgccctc ctgtttacca atttgtgtta
     2341 tgcccaggta atcgattata attttctcgg gggtggagca agggcgcgag ctatgggcgg
     2401 cgcgtttttt gctgtttcgg acgacccgtc agctggctcg tggaatcctg ccggattagc
     2461 acaattagat ctacctcaaa caaacctcac cttatatagc ggaagagtaa agattgaaca
     2521 cggtttgggt agcctttcca cagaagctaa gatgtcaaat gacttgatat tgtccggagg
     2581 tgtggcaatt ccgttcaagg tgagtgggta tcagatggtt ggttgcgttt cctatgaaag
     2641 actatcgatc ttttcagatc aatcttatta tgcaagtatc gataggtctg aggaaacaaa
     2701 tggaaatgtg aacgcggtga ctgtatccct tggaagagag cttatcaaag gactttccct
     2761 tggaatggca gtaaacatct attcaggcgg atatactttt aaggcatttc aagcggaccg
     2821 tccgtttaaa attggtgtaa gcgacacagt ttggagtgat acagtttttt cctatcatcc
     2881 cttaatggaa gcaaactatt ccggtttcaa tctgcaattc ggggcaatgt ataagatcca
     2941 gggactatca tttggtgcgg tggtcaaaac cccgctcac
//
LOCUS       DASWLN010000008         8437 bp    DNA     linear   ENV 20-FEB-2024
DEFINITION  MAG TPA_asm: Candidatus Zixiibacteriota bacterium isolate
            SMAG_U2949 k141_517958, whole genome shotgun sequence.
ACCESSION   DASWLN010000008 DASWLN010000000
VERSION     DASWLN010000008.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37960594
            Sequence Read Archive: SRR10376091
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Candidatus Zixiibacteriota bacterium (soil metagenome)
  ORGANISM  Candidatus Zixiibacteriota bacterium
            Bacteria.
REFERENCE   1  (bases 1 to 8437)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 8437)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (26-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/12/2024 08:28:26
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 1,440
            CDSs (total)                      :: 1,416
            Genes (coding)                    :: 1,412
            CDSs (with protein)               :: 1,412
            Genes (RNA)                       :: 24
            rRNAs                             :: 1 (16S)
            partial rRNAs                     :: 1 (16S)
            tRNAs                             :: 21
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 4
            CDSs (without protein)            :: 4
            Pseudo Genes (ambiguous residues) :: 0 of 4
            Pseudo Genes (frameshifted)       :: 0 of 4
            Pseudo Genes (incomplete)         :: 3 of 4
            Pseudo Genes (internal stop)      :: 1 of 4
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..8437
                     /organism="Candidatus Zixiibacteriota bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_517958"
                     /isolate="SMAG_U2949"
                     /isolation_source="soil"
                     /db_xref="taxon:2053527"
                     /environmental_sample
                     /country="USA: Oregon"
                     /lat_lon="42.99 N 123.63 W"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            110..553
                     /gene="mscL"
                     /locus_tag="VGB16_00190"
     CDS             110..553
                     /gene="mscL"
                     /locus_tag="VGB16_00190"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003387862.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008381 - mechanosensitive monoatomic ion
                     channel activity [Evidence IEA];
                     GO_process: GO:0034220 - monoatomic ion transmembrane
                     transport [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="large-conductance mechanosensitive channel
                     protein MscL"
                     /protein_id="HEX9916132.1"
                     /translation="MFKEFKEFAMRGNVLDMAIGIIIGAAFGKIITSLVNDILMPPIG
                     LLLGKLDFANLYFNLSGGKFASLIQAKAAGAVTISYGVFVNMVIDFVIVAFVIFLLVR
                     QVNRFKRQPAPVEPTTKECPYCFSAIPIKATRCPFCTSQLEKAKV"
     gene            681..1733
                     /locus_tag="VGB16_00195"
     CDS             681..1733
                     /locus_tag="VGB16_00195"
                     /inference="COORDINATES: protein motif:HMM:NF040521.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="C45 family peptidase"
                     /protein_id="HEX9916133.1"
                     /translation="MKIHDFSGSHFEVGVAIGKTFAERIRKTLADNITLQQIYLPFHR
                     SPEGKRAYQRLIQLHNSRFPQFVSELEGISEGAGVSLEELFLVNLRGEYKSFAKRSAD
                     FGCSTCSLLTSDKAIFAHNEDGASIYDGQMYLIRLQIVEELAFTAVCYPGFLPGNSCG
                     FNSEGICFSVNNVTPKGITVGLGKHFIARSLFEARSIGEAIQLITASGRASGFNHTIG
                     SIKERRIVNVEVSPTDYHVLEIKGHLFHANHYIMLRQEDQLISPSSQARQNRGESLLA
                     KGVAQDQAGILNLLRDSQVQDYPILRDGKSPDNGITLLTAVFDLDSKRFILYPGATEG
                     KKRGFEPLTQISMAPR"
     gene            1983..3959
                     /locus_tag="VGB16_00200"
     CDS             1983..3959
                     /locus_tag="VGB16_00200"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_015863078.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0016020 - membrane [Evidence IEA];
                     GO_function: GO:0022857 - transmembrane transporter
                     activity [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="APC family permease"
                     /protein_id="HEX9916134.1"
                     /translation="MENKPTFIQKLKTLFIGGSRSLNDPGLFHKISLIAFFAWVGLGA
                     DGITSSCYGPQEAFLALGGHHYLAIFVALGTAITIFVISTSYSQIVELFPTGGGGYLV
                     ASKLLSPNLGMLSGCALLIDYVLTITVSIASGADALFSFLPAGWYPFRLEVAILVVLM
                     LILLNLRGVKESVVPLVPIFLIFILTHVFVILYGLVINLNNIPTAVQATANQVQSVQS
                     EIGFLGMLFLILRAYSMGAGTFTGIEAVSNGLPILREPKVQTAKRTMRYMAFSLAFIV
                     LGLMIGYLLYGVEFAPGKTLNAVLFQKMTTNWGDAGYVFVLVTLLSEAVLLFVAAQTG
                     FLGGPRVLGNMALDRWFPTRFATLSDRLVTQNGILLMGGAAVILMLLTGGSVRFLVVL
                     YSINVFITFFLSQLGMVRHWWNDRANVKGWRRKLLVNGIGLVLTGFILVSVVVLKFHQ
                     GGWITLLVTGSLVGIAVIIKRHYIYNVRLLGRLDELVVATSSPAAKEEMTKAPQFDPK
                     AKTAVLLVNGFNGLGLHTLFSIIRLFGATYRNFVFLQVGTVDAGNFKGKAEIGSLENY
                     IKSELDRYVDFVKRKGFYAEGIHSIGTEVVDEIALIAPKVLERFPNAVFFGGQLVFPK
                     DTILSRWLHNYTAFAVQRKLYHEGIPVVILPIRV"
     gene            4464..4766
                     /locus_tag="VGB16_00205"
     CDS             4464..4766
                     /locus_tag="VGB16_00205"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_020261895.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="RNA-binding protein"
                     /protein_id="HEX9916135.1"
                     /translation="MNIYVGNLSQDTTDEDLKQAFGAFGQVTSASIIKDKYSGEPRGF
                     GFVEMPVKTEAQSALTEMNGKELKGRSLSVNEAHPRTEGGRQGGGRRGGGGGRRRY"
     gene            4897..5676
                     /locus_tag="VGB16_00210"
     CDS             4897..5676
                     /locus_tag="VGB16_00210"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_012548683.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="methyltransferase domain-containing protein"
                     /protein_id="HEX9916136.1"
                     /translation="MKTTITKDKTDKFVSEYEAVFEPKDYLYFYEKMTAPKITQKQIK
                     FLVRELGLDKPKKILDMPCGFGRHSNCLAKLGHKVTGVDLMPGFLEMAKACAKKMKVK
                     VDYIQADMRKIHLREKFDRIVILFTSFGYYTDEENFKVMKNMSNLLKIDGLLCFDTFN
                     RDAFLRGFLPSFVTQKGDDFMIDQNSFDSITGRLNVKRVVIRDGLRKDKPFSLRLYSP
                     TEMKELLKRAGFKLHQIYSDWNANPFTSDSRRMIIIAKKVR"
     gene            5955..7100
                     /locus_tag="VGB16_00215"
     CDS             5955..7100
                     /locus_tag="VGB16_00215"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_020258837.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="MFS transporter"
                     /protein_id="HEX9916137.1"
                     /translation="MEKNEATKTIRTFALASFLNDMGSDMIYPIWPLFVTTVLGANMT
                     ILGLIDGLGEAVVSISQAVSGYLSDRIRKRKIFIWSGYLCAFLSRIGYAISTTWQYLI
                     PFKILDRAGKIRAAPRDAIVADVCTRENRCEKFGLLGAMDSLGAVCGIVICILFYRLL
                     GYTNLFLIAAIPSAIGAILILSLIKERKPTDVKTYRGMSLKDVDANLKLFFLLSSIFA
                     FGSFSYSFLLIYAKHFGFGVTFVPVLYLVFTAVASIVSLPVGKLSDKIGRKPLLVLSF
                     VLWGLVCLSFILLQSHLVVIVTFVLYGLHKGSLDPVQKTLVSELSPTQFRASTLGGLK
                     MVTGICALPASLIAGLLWDKIGIFAPFYFSLGLTSLSVVMLLFVKEK"
     gene            7113..7724
                     /locus_tag="VGB16_00220"
     CDS             7113..7724
                     /locus_tag="VGB16_00220"
                     /EC_number="2.3.1.-"
                     /inference="COORDINATES: protein motif:HMM:NF012792.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008080 - N-acetyltransferase activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="GNAT family N-acetyltransferase"
                     /protein_id="HEX9916138.1"
                     /translation="MRKRKLLISGLKFYPLTPRRWKDFEELFGGRGACGGCWCMWWRL
                     GRSQFEKQKGKQNKLAMRKLVKSGQVPGILAYAGNKPIGWCSIGPREVYLSLIGSRFL
                     TKILEEENDKPIWSIVCFFVDRKFRNQGISIKLIQAALQFAKKKGARIVEAYPKQPKK
                     QRWPDAFVWTGHISAFKKCGFVETDRPSPSRPIMRYFISRKTK"
     gene            7742..8086
                     /locus_tag="VGB16_00225"
     CDS             7742..8086
                     /locus_tag="VGB16_00225"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_020253282.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF3795 domain-containing protein"
                     /protein_id="HEX9916139.1"
                     /translation="MEKLISFCGINCSACPAYIATQKDDDDERKKVAETWSKQYEHEF
                     KPEEINCDGCVVVEGRHISYCNDCEIRKCGLEKKVENCAWCNDYVCEKLSKFIEKAPD
                     LKKNLEEIRKSL"
BASE COUNT         2290 a         1774 c         1977 g         2396 t
ORIGIN      
        1 aaactgtaac ctatgtcccc agtctaaaat gtaacctatg tctcagttgc acagagattt
       61 ctgctctttg tggaggttat ccctctaatt catcaaccaa ggaggattga tgttcaaaga
      121 attcaaagaa tttgctatgc gggggaatgt gctggatatg gcaattggaa tcatcatcgg
      181 tgcggctttt ggcaaaatca tcacttcgct agtgaatgat attctgatgc cgcccattgg
      241 actgcttttg ggaaaattgg atttcgccaa tttgtatttt aacctctccg gtggaaaatt
      301 tgcctcttta atacaagcta aggcagctgg agcggttacc atcagctatg gtgtattcgt
      361 caacatggta attgattttg tcatcgtggc ttttgttatt ttccttctgg taaggcaagt
      421 aaataggttt aagcgtcagc cagcaccagt tgagccaacc acaaaagaat gtccatattg
      481 tttttcggct attcctatta aagccacgcg ctgtccgttc tgcacctctc agttggagaa
      541 agctaaagtc tgatcggaca actgctggcc acttcacaga aacaaaaata agggctgagc
      601 caattgacaa gaaaggaccg tcagtcatgt gtccgaggat aagtctacct ccaaggtcaa
      661 cttgatggcg gcaagctgat atgaaaatac acgatttttc tgggtctcac ttcgaagttg
      721 gagtggccat aggaaaaact tttgccgaac gaattcgaaa aactctggcg gataatatca
      781 ctttacaaca aatctatctc ccatttcacc gctcaccgga aggcaaacgc gcataccaaa
      841 gattgattca attgcacaac tcccgatttc cacaatttgt ttctgaactg gaagggattt
      901 ccgaaggagc tggggtatcc cttgaagaac tgttcctcgt caacttacgt ggggagtaca
      961 aaagctttgc aaaaaggtcg gcggatttcg ggtgttccac ctgctcgctt ctcacctccg
     1021 ataaagccat ctttgcccac aatgaagacg gggcatctat ctatgatggg caaatgtatt
     1081 tgattcggct tcagattgtt gaggagcttg ctttcacagc agtctgttat ccaggatttc
     1141 ttccaggaaa ctcttgtggt tttaacagtg aaggcatttg tttttcggtc aacaatgtca
     1201 cgcccaaggg aattactgtt ggtttaggca agcatttcat cgcgcgatct ttgtttgagg
     1261 caagatccat cggagaagca atccagctta taactgcctc gggacgcgct tcgggcttca
     1321 accatacaat cggttctatc aaagagcgcc gcatcgtaaa tgtagaggtt tctccgacag
     1381 attatcacgt cttggagatc aaaggacatt tgtttcacgc aaatcactac ataatgctcc
     1441 gccaagagga ccaactaatt tcgccttcca gccaggccag acaaaaccga ggggaatcgc
     1501 ttttagcaaa aggagtggct caagatcaag ccggcatatt aaatttgcta agagatagcc
     1561 aggtgcagga ttatccgata cttcgggatg ggaagtcgcc agacaatggc ataacccttc
     1621 taactgctgt cttcgacctt gattccaaaa gattcatact atatccagga gctactgaag
     1681 gaaagaaacg tgggtttgaa ccgctaaccc agatctccat ggcgccacga tagcggacta
     1741 cgataactac gtacatattt ctggtctcca atggcaagcg caaccctctc ccctacttca
     1801 gcttgtcaaa actatccaga aatgacttga cttttgaacc aaagcaatta aaattaaggc
     1861 aatagattct aacctatatt cttttacgcc gcttcgcaaa ctttacagtt tgcgataagt
     1921 ttttatcccg ataaatccga atatgaagtc aaaaggtata ttttaggggt gattttgtgc
     1981 aaatggaaaa taaacccact tttattcaga agctaaagac tcttttcatc ggtggctcgc
     2041 gcagtcttaa tgaccccggc ttatttcaca agatttcttt aattgctttc ttcgcctggg
     2101 tgggattggg agctgatggc atcacctcct cctgttatgg accacaggag gcttttcttg
     2161 cgctcggagg acaccattat ttagctatct ttgtggctct gggcacagcc atcaccatct
     2221 ttgtcatcag taccagctac tcccagatag tcgagctttt ccccacaggc ggtggtggat
     2281 atttagtcgc cagcaagctt ttatctccaa accttggtat gctttcaggg tgtgcccttt
     2341 taattgacta tgtgctcacc atcacggttt caattgccag cggtgcggat gctcttttca
     2401 gttttcttcc tgcaggatgg tatccattca gacttgaagt tgcgattctg gtagttttga
     2461 tgcttattct tttgaacttg cgaggggtta aggagtctgt ggttccttta gtgcctatct
     2521 ttttgatttt tattctcacc catgttttcg tcatcctata tggtctggtg atcaatttga
     2581 acaacattcc cacagcggtg caggcaacag caaaccaagt tcaaagcgtc caatctgaaa
     2641 tcggttttct tggaatgctt ttcttgattc tgcgtgccta tagtatgggt gctggaacgt
     2701 ttaccggaat cgaagcagtc agcaacggcc tccccatact aagagagcca aaagttcaaa
     2761 ccgccaaacg cacaatgcgt tatatggcat tttccttggc tttcattgtg ctgggtttga
     2821 tgattgggta tcttttgtat ggggtcgaat ttgcaccggg aaagacttta aatgctgttc
     2881 tcttccagaa aatgacgacc aattggggag atgctggtta tgtttttgtg ttggtgactt
     2941 tgctttccga ggcggttttg ctctttgtgg cagctcagac aggttttctt ggtggtccac
     3001 gggtattggg aaatatggct ttggaccgtt ggtttcctac aagatttgcc accctaagtg
     3061 accgcttggt tacccagaat ggaatccttt tgatgggtgg cgcagcagtc atcttgatgc
     3121 tcctcaccgg cggctcggtt agatttctgg tagtgcttta cagtatcaac gtcttcatta
     3181 ctttcttctt atcacaattg ggaatggtta ggcattggtg gaacgaccgt gccaatgtca
     3241 agggttggag gagaaaactt ttggtcaatg gcatagggtt agtgcttacc ggtttcattc
     3301 tggtatcagt cgtggttctt aagtttcacc aaggtggctg gatcaccttg ctagttactg
     3361 gaagtctggt cgggatagca gtcatcatca agcgccatta tatatataat gttcgcctct
     3421 tgggtagatt agatgagttg gtcgtggcaa cttcttcccc tgccgcaaaa gaagagatga
     3481 caaaagctcc gcaatttgac cccaaagcca agaccgcagt cctcttggtt aatggattta
     3541 acggcttagg gcttcatact cttttcagca ttatccgttt gtttggagca acatatagaa
     3601 acttcgtttt ccttcaggta ggcacagtgg atgccggaaa cttcaaagga aaagctgaaa
     3661 taggaagtct ggagaattac ataaaaagtg agctggaccg ttatgttgat ttcgtaaaaa
     3721 gaaaaggttt ttatgctgaa gggattcatt ctattgggac tgaagtagta gacgagattg
     3781 ccctgatcgc acctaaagtt ttggagcgtt ttcccaatgc ggttttcttt ggcggacaat
     3841 tggttttccc caaagatacc attctctcac gttggcttca taactacact gcctttgcgg
     3901 tgcagagaaa actttaccac gaagggattc cggtagtaat attgccaatt agagtgtaag
     3961 tttattaaat aagaatttct ttctaatgtc attcccgcga aagcgggaat ccagtcttaa
     4021 aaccaaatgg attcctgcct acgcaggaat gataaaaaag ttgtatcgcg caggagcgga
     4081 gaatcgaata gtgcagggct aaatgccctg cactacgaaa ttcatagtgt gtttacgtag
     4141 ggatgggaat ttccttcgac cttgctcagg acagtgaaga cgttcggctg agcggttcga
     4201 ccgtcccgag cggctcaccg tcgaaggact cagtcgaagc cctcgtcgaa ccgttgaacc
     4261 atattccacc cttatttccc ctttcctaat gtgtgatttg tatgtagaat ccatatgtct
     4321 ctgttgaaaa cctcttgact ttcgtaaaag aataagcgag attgaaacaa aaccttaact
     4381 tgaccttacc cgaatttttg aaaggtaacg taaaagttag gccaaatcac aaaataaatc
     4441 tttaaatcaa aaaggaggtg agaatgaata tttacgtggg caatctatcg caagacacta
     4501 cggatgagga tttgaagcaa gcttttggag ctttcggcca ggtaacatcc gccagcatta
     4561 tcaaggataa atacagtggt gaaccgagag gattcggatt cgtggagatg ccggttaaga
     4621 ccgaagcgca atccgccctt accgaaatga atggcaagga gcttaaagga aggtcgctta
     4681 gcgtcaacga agcccatcct cgcactgaag gaggaaggca gggcggagga aggcgaggag
     4741 gcggaggcgg cagaaggcga tattaaaaag ccagattcgg atttaacgaa atcgcaataa
     4801 aagttttact ataacgatat atctatagtc gtcccgctga gaacggagct tacccaagtt
     4861 ccgcttttgg cgggactttg taaatctcac aagaaaatga aaaccacaat aactaaagac
     4921 aaaaccgata agttcgtatc cgaatacgaa gcggtgtttg agccaaagga ttatctttac
     4981 ttctatgaga aaatgacagc tcctaagata actcagaagc aaataaagtt cttggtaaga
     5041 gagcttggac ttgataaacc caaaaagatt ctggatatgc cttgtggctt cggaagacat
     5101 agcaactgtt tggcaaaatt aggacataaa gttacgggtg tggaccttat gcccggcttt
     5161 ctggaaatgg ctaaagcttg tgccaagaag atgaaggtga aagttgatta cattcaagcc
     5221 gacatgcgta aaattcactt gagggaaaag tttgacagaa ttgttattct tttcacttcg
     5281 ttcggatatt acacggatga agaaaatttc aaagttatga aaaatatgtc caatcttctc
     5341 aagattgatg gcttattgtg ttttgatact tttaaccgcg atgctttctt gagaggcttt
     5401 cttccctctt tcgtcacaca aaagggagac gatttcatga tagaccaaaa ctctttcgac
     5461 agcatcaccg gccggcttaa tgtcaaaagg gttgtgattc gagacggact gcgcaaggat
     5521 aagccttttt ctttgcggct ctacagtcct acggaaatga aagaactatt gaagcgggca
     5581 ggattcaaac ttcaccaaat atattcagat tggaatgcca atccatttac cagcgattcc
     5641 agaagaatga ttataattgc caagaaggta aggtgaactt ctccgcctaa aggccgcgca
     5701 tccaaagcat ctcctccccc ttgagggggg attaagttga aaatcccgcc aaaggcggag
     5761 agagggtgag aattgtgttc gctatccccc tcaccccttc gcttcgccct gcaccagact
     5821 ggtgcagggc ttcgctcagg gcaggctcta actctctccc ctaaggggag aggaaactta
     5881 tagatatgtc atttgtcccc ctacggggat attctgacat atcttttata aatattaacc
     5941 agcaggttat gattatggaa aaaaatgaag ccacaaaaac aatcaggact tttgccttag
     6001 cttcatttct gaacgacatg gggtcagata tgatttatcc catctggcca ttgtttgtaa
     6061 ccacggtctt aggtgccaat atgaccatac tgggtttaat agatggtctg ggtgaggcag
     6121 tggtatctat atcccaagcc gtctccggtt atctatctga ccgcataaga aaaaggaaaa
     6181 tattcatttg gtcaggttat ctatgcgcct tcttgtctcg cataggttat gctatctcga
     6241 ccacttggca atatttgatt ccctttaaga tcctggaccg ggccggtaaa ataagggctg
     6301 cccccagaga tgccatcgtg gctgatgtgt gcacaagaga aaacagatgt gagaagtttg
     6361 gactactggg ggcaatggac agtctcggtg cagtatgcgg tattgtaatc tgcatcctat
     6421 tctacagatt attgggctat acaaacctgt ttctcattgc ggcaatacca tccgccattg
     6481 gagccatact gattctctcc ctaataaagg aaaggaagcc cacagatgtt aaaacctaca
     6541 gaggaatgag tctgaaagat gtagacgcta atctcaagct gtttttcctg ttgagttcga
     6601 tttttgcctt cggttctttc agttattctt ttcttttgat atatgccaag catttcggtt
     6661 ttggagtaac ttttgtgccg gtgttgtatc tggtatttac tgctgtggcc tctattgttt
     6721 ctctgcccgt gggcaaactc tcagacaaaa taggcagaaa gccgttgctt gtgctctctt
     6781 ttgtcctctg gggactggta tgtttgagtt ttattttgct tcaaagtcat ctggtggtga
     6841 ttgtgacctt tgtcctctac ggtttgcata aggggtcact ggaccctgtc cagaagacgc
     6901 tcgtttcaga gctttctccg acccagttcc gggctagcac tttgggtgga cttaagatgg
     6961 taaccggcat ttgtgctctg cccgcttcgc tgattgccgg cttgctctgg gataaaatcg
     7021 gaatattcgc tcctttctat ttttcgctgg gattgacttc tctatctgtg gttatgttgc
     7081 tttttgtcaa agaaaaataa aaagccaaat ttatgaggaa gcgtaaactg ctcatttccg
     7141 gcttgaagtt ctatccactg actccccgaa gatggaaaga cttcgaagaa ctttttgggg
     7201 gaaggggtgc ttgtggtgga tgctggtgta tgtggtggag acttggcagg tcgcaatttg
     7261 aaaagcaaaa aggaaagcaa aataaattag ctatgagaaa acttgtcaag tcaggtcaag
     7321 ttccgggaat cttggcttat gctgggaata aacctattgg ctggtgttca attggtccaa
     7381 gggaagtata tctctcactt atagggtcgc gattcctcac aaaaattcta gaggaagaaa
     7441 acgataaacc gatctggtca attgtttgct ttttcgttga cagaaagttt agaaatcaag
     7501 gcatctccat caaactgatt caggcagctc tccagtttgc caaaaagaaa ggggcacgaa
     7561 tcgtggaagc atatcccaag cagccaaaaa aacaaagatg gcctgatgct tttgtctgga
     7621 ctggtcatat atccgctttc aaaaagtgtg gctttgtgga aactgatcgt ccttcaccca
     7681 gccgaccgat aatgagatat ttcatttcaa ggaaaacaaa ataaaatgga ggaacaagtt
     7741 tatggaaaag ctaatttcct tttgtggaat taactgctct gcctgcccgg cttatatagc
     7801 tactcaaaaa gatgatgacg atgaaaggaa aaaagtggca gagacgtggt ccaagcaata
     7861 cgagcacgag ttcaagccgg aagaaattaa ctgcgatgga tgtgtcgtcg tagagggacg
     7921 gcatatcagt tattgcaatg actgcgagat aagaaaatgc ggtttggaga agaaagtcga
     7981 gaattgtgct tggtgtaatg attatgtttg cgagaaatta agcaaattta tcgagaaagc
     8041 accggacttg aaaaagaatc tggaggagat caggaagagt ttgtaatgga aatgaaaacg
     8101 ttgggtaggg aacggctgcg ccgttcccga aaaagtaatg gcagcagcca ttacttaccc
     8161 ggtttagaaa agcaacctcc tctcccttga gggagaggat aaaggtgagg gtgaaggttt
     8221 tttcttttct ccccctcacc ccttcgcttc gccctgcatc aatctggtgc agggcttcgg
     8281 tcagggcaag ctctatacct ctccccgatg gggagaggga actaaaagaa cgctattcat
     8341 ccctgtgcaa agcaccgggc attctggcat attttcgtaa atcataggcg acccagacgg
     8401 gtcgccttct tcatttgaag gggttggttc atccaag
//
LOCUS       DASWLN010000009         6200 bp    DNA     linear   ENV 20-FEB-2024
DEFINITION  MAG TPA_asm: Candidatus Zixiibacteriota bacterium isolate
            SMAG_U2949 k141_132125, whole genome shotgun sequence.
ACCESSION   DASWLN010000009 DASWLN010000000
VERSION     DASWLN010000009.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37960594
            Sequence Read Archive: SRR10376091
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Candidatus Zixiibacteriota bacterium (soil metagenome)
  ORGANISM  Candidatus Zixiibacteriota bacterium
            Bacteria.
REFERENCE   1  (bases 1 to 6200)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 6200)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (26-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/12/2024 08:28:26
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 1,440
            CDSs (total)                      :: 1,416
            Genes (coding)                    :: 1,412
            CDSs (with protein)               :: 1,412
            Genes (RNA)                       :: 24
            rRNAs                             :: 1 (16S)
            partial rRNAs                     :: 1 (16S)
            tRNAs                             :: 21
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 4
            CDSs (without protein)            :: 4
            Pseudo Genes (ambiguous residues) :: 0 of 4
            Pseudo Genes (frameshifted)       :: 0 of 4
            Pseudo Genes (incomplete)         :: 3 of 4
            Pseudo Genes (internal stop)      :: 1 of 4
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..6200
                     /organism="Candidatus Zixiibacteriota bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_132125"
                     /isolate="SMAG_U2949"
                     /isolation_source="soil"
                     /db_xref="taxon:2053527"
                     /environmental_sample
                     /country="USA: Oregon"
                     /lat_lon="42.99 N 123.63 W"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            572..1864
                     /locus_tag="VGB16_00230"
     CDS             572..1864
                     /locus_tag="VGB16_00230"
                     /inference="COORDINATES: protein motif:HMM:NF024583.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ATP-binding protein"
                     /protein_id="HEX9916140.1"
                     /translation="MAEGPDILQRKMNYLVQSNQELKRRLFDLNTVFEISRSLNSILD
                     VDTLLENVLDACVAQLEIDGAAIVIQRRNGKEHLSLIKTKNLETNEKLDLRFDCEGEL
                     VRLLKSSGKHMSKNQIAKHLDFSSSDFKKLEALGCQICVPLICKNQMRGVLFLSKKIE
                     GKEFEEKDLEFITMLTEQMAAAIENAVLYESERKSLEQLKNAQRQLIRSEKLVAIGQL
                     SASLAHEINNPLGIIKNYLALTQNSLSPQDKNRANLGIIEEELDRIARIVRQLLDLCQ
                     PRNEKMLPADLPAIVEQTISLVEKQFEKNKISIVRQFHENLPTLEGYSDQLKQLFLNL
                     LMNAQDSMPQGGKIHIAVSKNEDQIRICFSDTGCGIPKENISRVFEPFFTTKADKKGT
                     GLGLWICLEIINQHNGTISLENRSEGTGFIINLPIKQR"
     gene            1872..3962
                     /locus_tag="VGB16_00235"
     CDS             1872..3962
                     /locus_tag="VGB16_00235"
                     /inference="COORDINATES: protein motif:HMM:NF024583.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005515 - protein binding [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="GAF domain-containing protein"
                     /protein_id="HEX9916141.1"
                     /translation="MSKDRPKILVIDDETRMCQSLESLLANDGYHVIVADAGNKGIDC
                     IRENQLDLVISDIKLPDVDGIEILRVAKTKDENLPVILMTGYASLETAIEAVEQGAYD
                     YLMKPIEFSHLQLVIKRGLEKRELNLAKTILLTQLQLRNEQLQKKVEELNALYQAGKS
                     LSTTDSLDNLLDQIIHLATAVIGAKIGSIMLFQPPENHLVIKAAIGLEEEVIKTTRLG
                     PGQGIAGYVAEKGIPLLVRDIEEDIRFKRINKQKYETKSLLSVPLQVKDRVLGVINLS
                     NKQDKKLFTREDLRLLATFASQAAIAIDDAYHFEEKLQRIGELSALYEIATHLSVTEQ
                     TSQVADFVFEQLRRVMPIDFCLWFDFDQKGKLLRFAFQKGCKKETIDALRTIEFGLEK
                     SDILNSDKLNKKVKAELKSLPHLEEYLDSIHSTGILAEDTVHGILCIGNYQHKPISKQ
                     QADLISIVASQISIIYERQRAVLNASRLVTMGNMLSEISHDLKKPLTNIKGALQLLRE
                     KKKEKYLLQAEEEIKHLTDLVKEMVDFSNPNKYELVQVDIKSIVEKALSLVKNDLETG
                     GIKFEANYGDDLPLVFANRNEILEMILNIILNALESMPQGGSLKIETNKQYLSEKQEE
                     FLKIAIRDTGCGIPRENLNKIFTRYFTTKKEGTGLGLAIVDRAIKAHGGFVEVESELG
                     KGTTVMLYLPLSHT"
     gene            4035..5429
                     /locus_tag="VGB16_00240"
     CDS             4035..5429
                     /locus_tag="VGB16_00240"
                     /inference="COORDINATES: protein motif:HMM:NF025885.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_process: GO:0006355 - regulation of DNA-templated
                     transcription [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="sigma-54 dependent transcriptional regulator"
                     /protein_id="HEX9916142.1"
                     /translation="MIKDKTKLLVIDDDPKVSWILNEGLGENYEIISAKDGQTGLEIL
                     SQNRPDLVLLDIKMPGMEGLEVLEKIRSFDHLVDVIMLSGHGETKNVVESIKLGAAEF
                     INKPFDVKELEIHIQNVLEKSRLKKELTQLRSELRAKNAYDSLVGDSPVMLQIRSIVE
                     QVADSELTVLIRGNSGTGKEIVARLLHSLSSRKNEPFIKVNCAAIPRELLEAELFGYE
                     KGAFTGAHKTKPGRFEIASKGTIFLDEIGDMPLELQSKLLQVLEQCEFMRVGGIKTIR
                     VDVRIVCATNRDLEQALKRKALREDLFYRLNEVTITLPLLKDRKSDIPLLMEHFLQKY
                     NSLYNKKYGQISTANMKLIHEHDWPGNVRQLENLIKQVVVRGDEEIIHEIIKSTPSEH
                     NAEVILEEKNSVQKETFSLKEKVATTITKEEKKLIAEVLNKTNWNRRKAAELLEISYR
                     SLLYKIKEYQLNNS"
     gene            5789..>6200
                     /locus_tag="VGB16_00245"
     CDS             5789..>6200
                     /locus_tag="VGB16_00245"
                     /EC_number="2.7.7.65"
                     /inference="COORDINATES: protein motif:HMM:NF013180.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="diguanylate cyclase"
                     /protein_id="HEX9916143.1"
                     /translation="MKEKKCEATDPLVEFHRPHKFFGLRTWQELKRAERYCEFLSLMV
                     IDISSLSKFSQKKNKTEQSPKELEESLERIIRKNVRETDIVSRFEKNRLALLLSETPK
                     EGASCVCRRLEEEIRAYASDILMTPQDWKTHIEIV"
BASE COUNT         1988 a         1201 c         1429 g         1582 t
ORIGIN      
        1 ccttggccca tcttgcctgc ccggccaggg aggacaagat gggcttaccg ttcgcctgca
       61 aaaaaataaa cccttcgtag tgcggggaat ttatccccgc acggtctggg atgaattccc
      121 agccctacaa gacaaatgga aatgtagagg cgtatcacag tatcacaata cgtctctact
      181 aatttaccaa ccatcgcctc taacagcaat aaaaaattgg aggccttctt tgttaatctc
      241 tttacacctt tctgccgaat taaaggaata gcggcgggtg gcccacagct ctgctgtggg
      301 gcacctgatt gacatattta caattcaatt gagtatatag actttgctct tcaattacgt
      361 agaggatatt agctaatgtc ccctactttt ctcttcgtaa caaaccttcc atagaagttc
      421 cggccatcag gggtagctcc tcacagaata gttagcttcc tgaaactgga aactgctttt
      481 aaaattaatc tatttacacc tatctgccga attaaaggaa tagcgggaaa atagatttcc
      541 gccaagggga tattttttct ttgaggtgaa aatggctgaa ggaccggata ttctgcagag
      601 aaaaatgaat tacttagtcc agtctaatca ggaacttaag cgtcggctct tcgacctaaa
      661 cacggtcttc gagataagcc ggagtctgaa ctccatcctg gatgtggaca cgctcttgga
      721 gaatgtgctg gatgcctgtg tcgcccaact ggaaatagat ggtgccgcca ttgttattca
      781 aaggaggaat ggaaaagaac acctcagttt aatcaagaca aaaaacttgg agaccaatga
      841 aaaattagac ctccggttcg actgcgaggg cgaattggta aggttattga aatcaagtgg
      901 caaacatatg tctaaaaacc agatcgccaa gcatctggat ttttccagct cggatttcaa
      961 gaagttggaa gcattaggtt gccagatttg cgtccccttg atatgcaaaa atcaaatgag
     1021 aggggttttg tttctttcca agaaaattga gggcaaggaa ttcgaggaga aagatttgga
     1081 attcataact atgttgaccg aacagatggc tgcggctatt gagaacgcag tgctttatga
     1141 aagcgaaagg aaaagtttag aacagttgaa gaatgcccag aggcaactca ttcggtcaga
     1201 gaaattggtt gccataggac agctttccgc cagcttggcg catgagataa acaatccctt
     1261 aggaataatc aaaaattatc ttgctttaac gcaaaattcg ctttcgcccc aagataaaaa
     1321 ccgtgccaat ctcggaatca ttgaggaaga gcttgaccgc atagctcgaa tagtcaggca
     1381 gcttttggac ctttgtcagc cgagaaacga aaagatgctg ccagcggatt tgcctgctat
     1441 agtcgaacaa actatatctt tggtggaaaa gcaattcgag aaaaacaaga tttccattgt
     1501 ccggcaattt cacgaaaatc tgccgacact agagggttac tctgaccagc tcaaacagct
     1561 ttttctgaat ttgcttatga atgctcagga ttctatgccg cagggtggaa agattcatat
     1621 tgctgtttca aaaaatgaag accaaatcag aatatgtttc agcgataccg gctgtggtat
     1681 tcccaaagag aacatctcca gagtctttga accttttttt acaactaagg ccgacaagaa
     1741 aggtactggg ctgggacttt ggatatgttt ggaaattatc aatcaacaca atgggactat
     1801 ctctctagag aatagaagtg agggaacagg gtttattata aaccttccaa taaaacaacg
     1861 gtagcttgat tatgtcaaaa gatagaccta aaattctagt catagatgat gagacacgaa
     1921 tgtgccaaag tctggaatcg ctcctagcta atgacggcta ccacgtgatt gtggctgatg
     1981 ctggcaacaa gggcatagac tgtatcaggg aaaaccagct ggacctcgtt ataagtgaca
     2041 tcaaactgcc ggatgtggac ggcatagaga tattaagggt ggctaaaact aaagatgaga
     2101 acctgccagt catcctaatg accggatatg cttccttgga aacagctatc gaggcggtgg
     2161 agcaaggcgc ttatgactac ttgatgaaac cgattgaatt ttctcatctg cagcttgtca
     2221 tcaaaagggg gctggaaaaa agagagttga atttagctaa aaccattctt ctgacgcaac
     2281 tgcaattgag aaacgaacag cttcaaaaga aagtggaaga gctgaatgct ctctaccagg
     2341 ctgggaaatc gctcagcaca actgatagtc tggataattt gctcgaccag ataatccatt
     2401 tggctaccgc agtcatagga gcaaagattg gctcaattat gctttttcaa ccaccggaaa
     2461 atcatttagt gataaaagcc gctattggac tggaagagga ggtaatcaaa actacccgct
     2521 tgggtccggg acaaggtata gccgggtacg tagctgaaaa gggtattcct cttctggtca
     2581 gagatattga agaggatatt cgatttaaga gaatcaataa gcagaaatat gaaactaaat
     2641 ccttgctttc cgtgccccta caggttaaag atagagtcct gggtgtgata aacctgagca
     2701 ataaacaaga taagaaactg tttacccgtg aagacctaag actccttgcc acctttgcct
     2761 cccaagccgc catagccata gatgacgctt accattttga ggaaaaactg cagagaatag
     2821 gagaattgtc agccttatac gaaatagcaa ctcatctttc tgttacagag caaaccagcc
     2881 aggtggcaga ctttgtcttt gaacagctaa gacgggttat gccgattgat ttctgtctat
     2941 ggtttgattt tgaccagaaa gggaagctct tgcgcttcgc ctttcaaaaa ggatgcaaaa
     3001 aggagacaat agatgcttta agaactattg aattcggtct tgagaaaagc gatattctca
     3061 attcagataa gcttaacaag aaagtcaagg cggaactgaa atctctgccc cacttggagg
     3121 agtatcttga ttccattcat tcaactggca tattggcgga agatactgtg catggcatcc
     3181 tctgtatcgg caattatcaa cataagccta tctccaaaca acaggcggat ttaatctcca
     3241 ttgtggcttc ccagatttcc atcatctatg aaagacagag ggctgtcctg aatgccagcc
     3301 gattggtgac tatggggaat atgctttctg aaatatcgca tgatttgaaa aaacctctaa
     3361 ctaatatcaa aggagcttta cagcttttga gggaaaagaa aaaggagaaa taccttcttc
     3421 aggctgaaga ggagataaag cacttgaccg atttggtgaa ggagatggtc gatttctcaa
     3481 atcctaacaa gtacgagtta gttcaagtgg atataaaatc gattgtagaa aaagctttga
     3541 gtctggttaa gaacgacctg gaaacaggtg gaatcaaatt cgaagccaac tatggtgatg
     3601 acctgccttt agttttcgct aaccggaacg aaattctgga gatgatttta aacataatcc
     3661 ttaatgctct tgagagtatg ccccaaggag gaagcctaaa aatagagaca aacaaacaat
     3721 atctgtcgga aaaacaggaa gagtttttga aaatcgccat cagggacacg ggatgtggca
     3781 tcccgcggga aaacctgaac aagatcttca ctcgatattt cactactaag aaggaaggaa
     3841 ctggtttggg tttggctatt gtggataggg ccatcaaagc tcacggtggc tttgtggaag
     3901 tggaaagtga actcggcaag gggactacgg ttatgcttta cttgccctta agccatacat
     3961 aattaaaaca aagtgtaatc ctgccgatac agacaaggaa gtggcacaac ctagatatat
     4021 ccttaaggtc aggtatgata aaagacaaaa caaagctttt agttattgat gacgacccta
     4081 aagtttcctg gattctgaat gaagggttgg gcgaaaatta cgagatcatc tcggccaaag
     4141 atggacaaac cggcttagaa atattatcac agaataggcc ggatttggtt cttctggata
     4201 taaagatgcc tggaatggaa gggttggaag ttctggaaaa gataagatct ttcgaccatt
     4261 tagtcgatgt gataatgctt tctggtcacg gcgagaccaa aaatgttgtg gagtcaataa
     4321 aactgggggc ggctgaattt atcaacaaac ccttcgatgt aaaagagcta gaaatccaca
     4381 ttcaaaatgt cttagagaaa agcaggctga aaaaggagct cactcaacta cggtctgagc
     4441 tcagagctaa gaacgcctac gatagtttgg ttggagatag tccggtgatg ctgcagataa
     4501 gaagcattgt agagcaagtg gcggacagcg aattaaccgt cttaatacga ggaaatagcg
     4561 gaaccggcaa ggaaatcgta gcgcgacttc ttcattcact ctccagccga aaaaatgagc
     4621 ccttcatcaa agtcaattgc gccgccattc cgcgagagct tttggaggcg gagttgtttg
     4681 gatatgaaaa aggggctttc accggcgctc ataaaaccaa accgggaaga tttgaaatcg
     4741 ccagcaaagg gacaattttt ctggacgaga ttggagatat gcccctggag ttacagtcca
     4801 aacttctgca ggttttggag caatgcgaat ttatgcgagt gggtgggata aagaccatca
     4861 gggtggacgt gagaatcgtc tgcgcaacta accgtgactt agagcaagct ctcaaaagaa
     4921 aagctttaag ggaagatctt ttctatcgac ttaacgaagt aaccatcact cttcctctat
     4981 taaaggaccg gaaatcggat attcccttgc tgatggaaca tttccttcaa aaatataatt
     5041 cgctctacaa caagaagtat ggccagatat ccactgccaa tatgaagctc attcacgagc
     5101 atgattggcc cggaaatgtg aggcaactcg agaacttgat taagcaagta gtggttagag
     5161 gggatgagga aataatccat gagataatca agagcacgcc ttcagagcat aatgcagaag
     5221 tgattttgga ggaaaagaat tcagtgcaaa aggagacttt ttctttgaag gaaaaagtgg
     5281 caaccaccat aaccaaagag gaaaagaaac tcattgctga ggtattgaac aagactaact
     5341 ggaacagaag aaaagctgca gagctcttgg agataagtta tcgttccctt ctttacaaga
     5401 taaaggaata tcaactcaat aatagttgaa ctcttttttc ttttttatgt aggtttgtgc
     5461 agtaattgtg aactatgttg cataagggct tttgcttaaa ggacgtagtt atcttaatag
     5521 tagttttagt agaacggaac ttttttctca aggaattaga aaaaccacaa accgttatgc
     5581 tcaagttgtt gtttgacaaa tgaataacct ttagtatatt tgattaagaa ttaaacaata
     5641 gggaagctgc tcgaattggt caaatcgagc ttttttgagt ggcacgcatt ttgcttgggg
     5701 aatagatagc tcaataaccc ttctatcttt tccccagcca aatttggctt ccttttgaac
     5761 ttgtcttttt taaaagcagg agcaaccaat gaaagagaaa aaatgcgaag cgactgaccc
     5821 attagtggag tttcaccgac cacacaagtt ttttgggcta aggacgtggc aggaactgaa
     5881 aagggcagaa agatactgcg agttcctctc cctgatggtc attgacatct caagtttatc
     5941 caagttctct caaaagaaga acaaaacaga gcaaagtccc aaagagctgg aagaaagctt
     6001 ggagagaata ataagaaaaa atgtcaggga gactgacatc gtttctagat ttgaaaagaa
     6061 ccgactagct cttcttctct ccgagactcc taaagaaggg gcaagttgtg tttgtagacg
     6121 cttggaagag gagataagag catacgcttc agatatactt atgaccccgc aggattggaa
     6181 aacccatatc gagatagtct
//
LOCUS       DASWLN010000010         3257 bp    DNA     linear   ENV 20-FEB-2024
DEFINITION  MAG TPA_asm: Candidatus Zixiibacteriota bacterium isolate
            SMAG_U2949 k141_1148547, whole genome shotgun sequence.
ACCESSION   DASWLN010000010 DASWLN010000000
VERSION     DASWLN010000010.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37960594
            Sequence Read Archive: SRR10376091
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Candidatus Zixiibacteriota bacterium (soil metagenome)
  ORGANISM  Candidatus Zixiibacteriota bacterium
            Bacteria.
REFERENCE   1  (bases 1 to 3257)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 3257)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (26-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/12/2024 08:28:26
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 1,440
            CDSs (total)                      :: 1,416
            Genes (coding)                    :: 1,412
            CDSs (with protein)               :: 1,412
            Genes (RNA)                       :: 24
            rRNAs                             :: 1 (16S)
            partial rRNAs                     :: 1 (16S)
            tRNAs                             :: 21
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 4
            CDSs (without protein)            :: 4
            Pseudo Genes (ambiguous residues) :: 0 of 4
            Pseudo Genes (frameshifted)       :: 0 of 4
            Pseudo Genes (incomplete)         :: 3 of 4
            Pseudo Genes (internal stop)      :: 1 of 4
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..3257
                     /organism="Candidatus Zixiibacteriota bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_1148547"
                     /isolate="SMAG_U2949"
                     /isolation_source="soil"
                     /db_xref="taxon:2053527"
                     /environmental_sample
                     /country="USA: Oregon"
                     /lat_lon="42.99 N 123.63 W"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            complement(<1..209)
                     /locus_tag="VGB16_00250"
     CDS             complement(<1..209)
                     /locus_tag="VGB16_00250"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEX9916144.1"
                     /translation="MARKIGCLVWIFIFIIAGLNSTWAKEINFSASVDRTELSLDDQI
                     TLTIQVSGDIGNIPTPTLPSLKDFD"
     gene            complement(214..966)
                     /locus_tag="VGB16_00255"
     CDS             complement(214..966)
                     /locus_tag="VGB16_00255"
                     /inference="COORDINATES: protein motif:HMM:NF024816.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005515 - protein binding [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="tetratricopeptide repeat protein"
                     /protein_id="HEX9916145.1"
                     /translation="MKLKKYKVVLYLIILLTLPSLVQSKSFSSLNNKGNKFYLKGDYQ
                     KALEMYKKAEADNPKSPTISNNLGNVAHQQGDFREAEKYYERAMENTNRLELANSVYN
                     LGNNYFRQGQMENALQSYQKCLELNPSDIDAKYNIEFIKRMQKQNQEQEQQESQQKEK
                     QSSQKENQRKKEEQKKEEEKKQEEKSSNSQTEKQKEEQKQQQQKFSESQPKMSKEEAE
                     RVLNAVKEQEKELLEKQKRFGEIKAGRVGKDW"
     gene            complement(969..1973)
                     /locus_tag="VGB16_00260"
     CDS             complement(969..1973)
                     /locus_tag="VGB16_00260"
                     /inference="COORDINATES: protein motif:HMM:NF012321.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="VWA domain-containing protein"
                     /protein_id="HEX9916146.1"
                     /translation="MRFTQPTYLYVTLGLVIALFLFYVLVSKLKKKAKQKLGDLELID
                     KLSVSFSPKKARWKKVLILLTIIFLGLTLARPQIGKKDVMVKRKGVDIVVCLDLSLSM
                     LSEDLKPNRLEKAKMELANLFSRMEGDRVGIVVFAGEAYVECPLTLDLSAAQMFLDAA
                     NVGLVPKPGTAIADAIDKGLELFDKREKKYKVMILLTDGEDHGSEPVEAAKRAKEEGV
                     IIYTIGIGSTQGEPIPLTDKGGSRVGYKEDEKGEMVLSRLNPQTLREIAENTGGKYFI
                     ASAGEMEIERMYEEISHMEKKELQDKLFTLYQDKYQYFLVFSLLFLVIESFISERRRG
                     "
     gene            complement(1978..2970)
                     /locus_tag="VGB16_00265"
     CDS             complement(1978..2970)
                     /locus_tag="VGB16_00265"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_006929313.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="VWA domain-containing protein"
                     /protein_id="HEX9916147.1"
                     /translation="MFRLAQPIFLILALLSIGIVVYHLILRRQRPASIRYSDLSLTNH
                     LKESSRSRETKILPLFRGLAVFFLVLALARPQSGLKSQEITSEGIDIMLVLDISGSMR
                     AEDFSAQNRLYAAKQVIEEFISERQTDRIGLVIFSRQSFLQCPLTLDYKILKELVEKV
                     DFGMIEDGTAIGLAIANAVDHLQLGKGKSKITILLTDGINNSGEIDPITATRVAQAMN
                     IKIYTVGAGKPGRALYPVEDPIFGKRYVYLPNELDEKMLQEIANITGGEYFRAKDEKG
                     LSQIYKEISQMEKTKIKIKEYVEYNELFSNFALFGLILVLGEIFLSNTRYRRIP"
BASE COUNT          797 a          795 c          592 g         1073 t
ORIGIN      
        1 atgtcaaaat cttttaagga tggaagcgta ggtgtgggaa tatttccaat atccccggaa
       61 acctgaatgg tcaaagttat ctggtcatct aagctcagtt cggtcctgtc aactgaagct
      121 gagaaattta tctcttttgc ccaggtcgaa ttcaaacctg caatgataaa aatgaatatc
      181 cagaccaaac aacctatttt ccttgccata aatctaccaa tcctttccaa ctcgccctgc
      241 tttgatctca ccaaatctct tctgcttttc taaaagctct ttctcctgct ccttgactgc
      301 gtttaatacc ctctccgcct cttccttgct cattttcggc tgagactcag aaaacttctg
      361 ttgttgttgc ttctgttctt ctttttgttt ttcggtttgt gaattggaac ttttttcctc
      421 ttgctttttt tcttcttcct tcttctgttc ttcctttttc ctttgatttt ccttttgaga
      481 gctctgtttt tctttctgct gggattcttg ttgttcctgc tcttgattct gtttttgcat
      541 cctctttata aattcgatat tatatttggc gtctatatcg ctcggattaa gctccaggca
      601 tttctggtaa gattgcaggg cattctccat ctgtccctga cgaaaatagt tgtttccaag
      661 attgtaaaca ctgttagcta actccagcct gtttgtattc tccatggcgc gctcgtaata
      721 cttctctgcc tccctgaaat ctccttgttg gtgagctaca tttcccagat tatttgatat
      781 tgttggcgac ttcgggttat ccgcttcagc tttcttgtac atttccaaag ctttttgata
      841 atctcctttc agataaaact tgttcccttt gttgttcagg gaggaaaaag atttggattg
      901 taccaagctg ggcaatgtca atagtattat tagatataat acaactttgt attttttaag
      961 tttcaagcct atcctctcct tctttctgaa atgaaagatt ctattaccag aaacaacagt
     1021 gagaaaacca aaaagtactg atacttgtcc tgatagagag tgaagagctt atcttgtaat
     1081 tcctttttct ccatatgaga aatctcttca tacatcctct ctatttccat ttctcctgcg
     1141 ctggcaatga agtatttccc tccagtattc tccgctattt ccctcaaggt ttgtggattc
     1201 aatctgctta aaaccatctc ccctttctca tcttccttgt aaccaactct gctgccacct
     1261 ttgtccgtca ggggtatggg ctctccctgg gttgaaccga ttcctatggt gtagattata
     1321 actccttcct ccttagctct tttcgccgct tcaactggct cacttccatg atcctcccca
     1381 tctgtcaaca gaatcataac cttatatttt ttctctcttt tgtcaaatag ctcaagtcct
     1441 ttgtcaatag catctgcgat ggctgtccct ggtttaggga ccaatcccac attcgccgca
     1501 tccaggaaca tctgagcggc tgatagatcc agagttaagg gacactctac ataagcttca
     1561 cctgcgaaaa ccacaattcc caccctatct ccctccatcc ggctaaagag attagctaat
     1621 tccatttttg ccttttctaa acgattgggc ttgaggtctt cggataacat cgaaagtgac
     1681 aggtctaaac agaccactat atccactccc tttcttttta ccataacatc tttctttccg
     1741 atttgcggtc gggctaaagt caagcccagg aatatgattg taagcaatat tagaaccttt
     1801 ttccacctgg ccttttttgg actgaatgat acggaaagtt tatcaattag ctccaaatca
     1861 cccagtttct gtttggcttt cttcttcaat ttggacacca gaacatagaa aagaaacaaa
     1921 gcaataacca agcccagagt cacatataga tacgttggtt gagtgaatct cattttttca
     1981 aggtatcctg cgataacggg tgttacttaa aaaaatctca cctagaacaa gaattaagcc
     2041 gaacagggcg aagttggaaa agagttcatt gtattccacg tactctttga tttttatctt
     2101 agttttttcc atctgactta tctctttgta tatctggctt aaaccttttt cgtcctttgc
     2161 gcgaaaatac tcaccgccgg ttatattagc tatctcttgc agcatcttct cgtctaattc
     2221 attgggaaga tagacatatc ttttgccgaa gattgggtct tcgactggat aaagtgccct
     2281 acccggttta cctgcaccaa cggtataaat ctttatgttc atcgcctgag ctactcgagt
     2341 tgcggttata gggtcaatct ccccggagtt gttgatccca tctgtgagaa gaatggttat
     2401 tttgcttttc cctttaccca gctgcaggtg atccaccgca ttagcgatag ccaatccaat
     2461 cgccgtccca tcttcaatca tcccgaaatc aaccttttca accaactcct ttagtatctt
     2521 gtagtctaaa gtcaaaggac attggagaaa actctgccgc gaaaatatca ccaagccgat
     2581 gcggtcggtc tgcctctcag atatgaattc ctcaatcacc tgcttggcag catataatct
     2641 gttctgtgca gagaagtctt cagccctcat gcttcctgaa atgtccagaa ccagcattat
     2701 gtcaattccc tccgaggtga tttcctggct tttcagacca ctttgcggcc gagccaatgc
     2761 caaaaccaaa aagaatactg ccaaacccct gaatagcgga agtatctttg tttctcttga
     2821 gcgggagctt tctttcaaat gattggtcaa actcaagtca gaatatctta tactggcagg
     2881 tctctgcctt cttaagatta agtgatagac tactatgcct atacttaaga gagccaaaat
     2941 caaaaatatg ggttgtgcca atctgaacat atctattttg cgattatgct aatacaaacg
     3001 cattgattta cgttcaatga atctcccctc gccctcgcct cgattcggcc cgcctcggcg
     3061 agccatcggc gagacgggcg aagcgggcct ctttttcgaa gaggggaaaa agaggtctcc
     3121 ccctttgaaa aagggggatt tgatggttcg atatgggctt cgactgagtc cttcgacggt
     3181 gagccgctca ggacggtcga accgctcagc cgaatgtcct caccatcctg agcaaagtcg
     3241 aagggagggg gatttta
//
LOCUS       DASWLN010000011         8113 bp    DNA     linear   ENV 20-FEB-2024
DEFINITION  MAG TPA_asm: Candidatus Zixiibacteriota bacterium isolate
            SMAG_U2949 k141_132866, whole genome shotgun sequence.
ACCESSION   DASWLN010000011 DASWLN010000000
VERSION     DASWLN010000011.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37960594
            Sequence Read Archive: SRR10376091
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Candidatus Zixiibacteriota bacterium (soil metagenome)
  ORGANISM  Candidatus Zixiibacteriota bacterium
            Bacteria.
REFERENCE   1  (bases 1 to 8113)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 8113)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (26-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/12/2024 08:28:26
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 1,440
            CDSs (total)                      :: 1,416
            Genes (coding)                    :: 1,412
            CDSs (with protein)               :: 1,412
            Genes (RNA)                       :: 24
            rRNAs                             :: 1 (16S)
            partial rRNAs                     :: 1 (16S)
            tRNAs                             :: 21
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 4
            CDSs (without protein)            :: 4
            Pseudo Genes (ambiguous residues) :: 0 of 4
            Pseudo Genes (frameshifted)       :: 0 of 4
            Pseudo Genes (incomplete)         :: 3 of 4
            Pseudo Genes (internal stop)      :: 1 of 4
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..8113
                     /organism="Candidatus Zixiibacteriota bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_132866"
                     /isolate="SMAG_U2949"
                     /isolation_source="soil"
                     /db_xref="taxon:2053527"
                     /environmental_sample
                     /country="USA: Oregon"
                     /lat_lon="42.99 N 123.63 W"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            complement(<1..904)
                     /locus_tag="VGB16_00270"
     CDS             complement(<1..904)
                     /locus_tag="VGB16_00270"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEX9916148.1"
                     /translation="MPRKVLISTLILLLTFGISAFGAEKKNAADQIMLERLKSMENAD
                     YPNENIVPRYITTPAPKLLGPLSSSVVGFTYYDYQHNDCMRRMIANGTDASNNIHVVW
                     MNSPDALLEDRWVSYNSYVEGSGWSGAVTVTNAAYRGGYTGLDLLPDQREVLCYHRTQ
                     PSTGAFWASAISIETDDPGVGTFESYDIPDSVSGQPPTLKGEWPTVASAKSTVDGKNY
                     IHITSAEGQTSGTVDKSMYYVRCYKGGSGTLICESPGWGTTLTINKDTKLAPNKLCYQ
                     FDRCRLGTGIMATSPVSEKVAIVYF"
     gene            complement(1026..1874)
                     /locus_tag="VGB16_00275"
     CDS             complement(1026..1874)
                     /locus_tag="VGB16_00275"
                     /inference="COORDINATES: protein motif:HMM:NF039201.3"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="FlgD immunoglobulin-like domain containing
                     protein"
                     /protein_id="HEX9916149.1"
                     /translation="MRKALLVVCLIAIVLLFGSSIVLAADVVVGIQKPAVIERSSEFI
                     VQVSVNSNQVSSGFQIPLSFFHNTNNDIRCDSIQWTSTFLNSKPTMYAGQAGDMSYLH
                     NESGVMAAVIWAVWYGEGLPKESGPICNLYFSTGPNWDPTVRLPIDLTDKLDPPGAAE
                     VSDKDGHVLLPTFKVNEGGNSGDVGGSALPTEFRLYQNYPNPFNPATTIEFNLPKGGP
                     VHLVIYNARGQKVKVFDQNLPQGTASVVWDGTDYSGKQVASGTYFYQVTANGQKLDAK
                     KMVLVK"
     gene            complement(2499..3473)
                     /locus_tag="VGB16_00280"
     CDS             complement(2499..3473)
                     /locus_tag="VGB16_00280"
                     /EC_number="2.5.1.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011483743.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016765 - transferase activity,
                     transferring alkyl or aryl (other than methyl) groups
                     [Evidence IEA];
                     GO_process: GO:0006535 - cysteine biosynthetic process
                     from serine [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="cysteine synthase family protein"
                     /protein_id="HEX9916150.1"
                     /translation="MKYHNNILELIGNTPLVKLNKVVDENCATVLAKLEFLNPLGSIK
                     ERMAYHIISQAEKEGKIKKGDVIVDCTSGNTGIGVALVASLKGYRSLFTIPNKQSQEK
                     INLLRALGAEIIITPADAPSDSPENSHNVAKRIAGERKDAFYLNQHDNLQNAQAHYLT
                     TGPEIWKQTKGKVNCVVAGIGTGGTLSGIGRFLKEKNKNIRIVAVDSIGSVFYDVIKN
                     NPITAPKAYMVEGIGSDTMTQALDLKVIDDVLRVSDKEAFLMARRLLKEEGLFAGGSS
                     GAAMVGAKKIAEELGKGKLVVTILPDSGSRYLSKIYSDKWMKEQGFLD"
     gene            complement(3477..3821)
                     /locus_tag="VGB16_00285"
     CDS             complement(3477..3821)
                     /locus_tag="VGB16_00285"
                     /inference="COORDINATES: protein motif:HMM:NF013098.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="VOC family protein"
                     /protein_id="HEX9916151.1"
                     /translation="MSNFLCHFEICTSNLKKAKSFYTQLFNWEMSSWGTEGTYETIKT
                     SQEPFGGLWKTQEGKGVLIYFKVDDIQQTLNKVKQLGGKVIQEKNEIPNVGFYGIFSD
                     TDGNQIGLFNEK"
     gene            3999..4964
                     /locus_tag="VGB16_00290"
     CDS             3999..4964
                     /locus_tag="VGB16_00290"
                     /inference="COORDINATES: protein motif:HMM:NF033577.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004803 - transposase activity [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="IS481 family transposase"
                     /protein_id="HEX9916152.1"
                     /translation="MPKHFLWSTIKTRLNWFEMAKEKNITYACKYFGISRKTFYKWYR
                     RYQGSGENPKSLQDQSRRPKTHPKLTSPEVASLIIKLRGETKYGPRRLQFYLKRDYAI
                     SLSLCGIWKVLSRAGLIAHYKRRRKRYQSYGKYVRYLGHKVQMDTKPLPRRKGQFDSR
                     DYQYVAKDVFTKLRFIKVYQELSVNNTVDFTRRAILFFPFKIKRIQTDHGTEFTYDML
                     SSTRIHPLDKLLKEQKIKHVLSPVATPRYNGQVERSIRTDLEEFYRPLGVVKDHATLS
                     KKLLSYLEYYNQSRPHMTLNMLTPLEKLHSTPGYENAKLNWRCYL"
     gene            complement(5081..5569)
                     /locus_tag="VGB16_00295"
     CDS             complement(5081..5569)
                     /locus_tag="VGB16_00295"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_012548083.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="QueT transporter family protein"
                     /protein_id="HEX9916153.1"
                     /translation="MKFSNKFIAQASIIAALYAALTILVFSPISYNPMQIRVAEVLTV
                     LPYITPAAIPGLFIGCIAANLVSPAGIIDIIFGSLFTLLAAFLTYFVSKTKKPILAPL
                     PPVLINAFGVSIYLHKLFQLSYLICVLSIAVGEVIACYVLGYPLLRLVLSNEKLKNIV
                     KL"
     gene            6176..7468
                     /gene="purD"
                     /locus_tag="VGB16_00300"
     CDS             6176..7468
                     /gene="purD"
                     /locus_tag="VGB16_00300"
                     /EC_number="6.3.4.13"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_008516834.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004637 - phosphoribosylamine-glycine
                     ligase activity [Evidence IEA];
                     GO_process: GO:0006189 - 'de novo' IMP biosynthetic
                     process [Evidence IEA];
                     GO_process: GO:0009152 - purine ribonucleotide
                     biosynthetic process [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="phosphoribosylamine--glycine ligase"
                     /protein_id="HEX9916154.1"
                     /translation="MRVLVVGSGGREHALAWKLSQSPLINKIYAAPGNGGLSILAECV
                     DIAVDDLEKLVDFAHKNNIDLTVVGPELPLVLGIVDLFQQKGLKIFGPTKKAAQIEGS
                     KSFAKEFMRKYHIPTGSFRIFTNKEEASDFVNSATYPLVIKADGLAAGKGVIVAEEKN
                     SARDAIEKIMVDKIFAEAGAKLVIEEFLVGEEVTVLAFTDGKNIVPMVPSRDHKRVYD
                     GDIGPNTGGMGAVAPTTIVSERKTQEIYDEILEPTISGLEREGCSFKGILYAGLMMTE
                     VGPKVLEYNCRFGDPETQVVLPLLKNDLAEILLSIIDGDLDLEKIEWEQKFAVCVALA
                     SGGYPDKYEKNIEIFGLNKLDKEKSRDLQIFHAGTRRSVDRFFTTGGRVLGITCVDES
                     MEKAIKRCYAAIEYIKFNGMHFRKDIGYKVSGLRKEYY"
     gene            7875..>8113
                     /locus_tag="VGB16_00305"
     CDS             7875..>8113
                     /locus_tag="VGB16_00305"
                     /EC_number="5.4.99.18"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_005602143.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0034023 - 5-(carboxyamino)imidazole
                     ribonucleotide mutase activity [Evidence IEA];
                     GO_process: GO:0006189 - 'de novo' IMP biosynthetic
                     process [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="AIR carboxylase family protein"
                     /protein_id="HEX9916155.1"
                     /translation="MMKPKVLIIVGSESDLEIMSKCEEMLAKFGISYKLEISSAHRNP
                     QKTIQLAKTAENKGYSLIICGAGMAAHLPGVVASHT"
BASE COUNT         2322 a         1788 c         1788 g         2215 t
ORIGIN      
        1 taaaatagac aatagcgacc ttctcagaaa ccggggaggt agccatgatt ccagtaccta
       61 acctgcatct atcaaattga tagcagagct tattgggagc aagcttggta tccttattaa
      121 tggttaaggt agttccccaa ccaggagact cacatatcag tgtcccgctc ccacctttat
      181 agcagcggac atagtacata gacttgtcta cggtgcctga ggtttgacct tcagcactgg
      241 tgatatggat atagtttttt ccatcaactg ttgacttggc agaagccacg gtaggccatt
      301 ctccctttaa agttggaggc tgtcccgaaa ctgaatcagg aatatcataa ctctcgaagg
      361 ttccgactcc gggatcatct gtctctatgg aaatagcgct tgcccagaaa gcacccgtgc
      421 ttggctgggt gcggtgataa cacaagacct ccctttgatc cggaagtaaa tccagcccgg
      481 tatatcctcc cctatacgcc gcattggtta cggtcaccgc gccggaccag cctgatccct
      541 caacatatga gttataactt acccagcggt cctccaacaa tgcatccggc gagttcatcc
      601 agacaacgtg tatattgtta gacgcatccg tgccattggc tatcatccgg cgcatacagt
      661 cgttatgctg gtagtcgtaa taggtaaacc caacaacgct gctcgacagt ggacctaaga
      721 gcttgggtgc tggggtggtg atatacctgg gtactatgtt ctcgtttgga tagtcagcat
      781 tctccatact tttcaaccgc tcgagcatta tctggtctgc agcatttttc ttttcagccc
      841 caaaagcact aatgccaaat gtcagaagca aaatcaaggt cgaaatcaaa actttccttg
      901 gcattccttc ctcctttctt gagtttagtt ttagtttctt tatctgtaaa acaactactt
      961 aaggtttaaa atcacctcct cgcattgata aatatttaag agcaaacttt gagttcaaga
     1021 cctctttact tgaccaatac catcttcttg gcgtcgagtt tctggccgtt tgcagttacc
     1081 tggtaaaaat acgtgccgct ggcaacttgc ttaccactgt aatctgtgcc atcccatact
     1141 acgcttgcgg tcccctgggg aagattctgg tcgaaaactt tgaccttctg tcccctggcg
     1201 ttataaataa ctaagtggac tggaccaccc tttggcaaat taaactctat ggtggtagca
     1261 gggttgaagg gattaggata gttctgatat agcctgaact ccgttggaag agcacttcca
     1321 ccaacatctc cagaattacc cccttcgttc accttaaagg tagggagcaa gacatggccg
     1381 tccttgtcgg acacctctgc tgcaccagga gggtcaagct tatcggtcaa atcaattggc
     1441 aacctaaccg taggatccca gttaggtccg gtgctgaaat acaaattgca gatcggaccg
     1501 ctttcttttg gcaacccctc gccataccac actgcccaaa ttactgctgc cataacacca
     1561 ctttcgttgt gaagatagct catatcccca gcttgtccag catacatagt tggcttgctg
     1621 ttcaagaatg tgctggtcca ctgaatagaa tcacaacgaa tgtcattgtt ggtattgtgg
     1681 aaaaagctaa gaggaatctg aaaaccgctt gagacctgat tactgttaac cgaaacctgg
     1741 actataaatt cgctgctgcg ttcaatgact gccggcttct ggattcctac tacaacgtct
     1801 gctgctaaaa caattgagct gccaaacaag aggacaatcg caataagaca aactacgagc
     1861 aatgcctttc tcactgtaaa cctcccttcc aaaggtttta gatttttttt atttccctcc
     1921 ctctgtcttt ttaggattgg tacagacaaa acaaaaacca cctccttgga agctagaaaa
     1981 attaaggaga tgttgcctta gctcgcgctt acttacgaag aactctgatt aacttgtcaa
     2041 gaaaaaatgt aggttgcaca accgaagaaa aactaccgat aaaactttag aagaaaaaga
     2101 acatcaagtg taaacagctc aattgagaaa ataacgtatc aaacccttgt ttgccacctg
     2161 tagttaatct tccgagaaat gatttgtcaa gtcttttttg atatttatca agtaaattct
     2221 cgaccaggtg aaaaaattct gaaagccagc caaagagcta ccgcgaaaaa ttatcagttt
     2281 gttgttgaag aatagtaggg gaaaaggagt tcaaaagaag atttaaatta tcctgattag
     2341 tctttaagta gtggattatc tttatgttac tgaaatcaac taatcccatc cacgagaaat
     2401 cagtttaaaa agcagtagtt tcacttcagg ggaaaagaat gtctcaaggg aaaggcccca
     2461 ccgaaggctg accctttttt tcgatgaata aaacgattct aatccaaaaa cccctgctct
     2521 ttcatccatt tatcagaata aatcttgctt agataccttg agccgctgtc gggaaggatg
     2581 gttaccacta actttccttt acccagctcc tccgctatct tctttgcacc aaccattgcg
     2641 gctccggagg agcctccagc aaacagacct tcttccttca gcagtctcct ggccatcaaa
     2701 aatgcttctt tatcacttac ccgaagcacg tcatctataa ctttgagatc caatgcttga
     2761 gtcatggtgt cgcttccaat tccctcgacc atatacgcct taggcgcagt tatcgggttg
     2821 ttttttatta catcgtaaaa gactgaaccg attgagtcga cggcgacaat tcttatattt
     2881 ttattttttt ccttcaagaa tctccctatg ccggagagag tgcctccagt cccaattcca
     2941 gccacaacgc agttgacttt tcctttggtt tgtttccaga tttccgggcc ggtggtgaga
     3001 taatgcgcct gggcgttctg gagattatca tgctggttta aatagaatgc atcttttctt
     3061 tccccagcta tgcgcttggc gacattatgg gagttttccg gggaatcgga aggcgcatcc
     3121 gctggggtga ttattatctc tgctcccaga gccctgagta aattgatctt ttcctggctc
     3181 tgcttgttgg gtatggtaaa aagcgacctg tatcctttga gtgaagctac caatgcaaca
     3241 cctatgccgg tattccccga ggtgcaatct acaatcacat cccctttttt tattttccct
     3301 tccttttcag cttgagagat gatatgataa gccatccgct ccttgatgct tcccaatgga
     3361 ttcaaaaact caagcttggc taaaaccgtc gcacaattct catccacgac tttgttcaac
     3421 ttcaccagag gcgtgttgcc aatcagttcc aggatattgt tatgatattt catattttat
     3481 ttctcgttaa agaggccgat ttgatttcca tccgtatcag agaaaattcc ataaaagccc
     3541 acatttggaa tttcgttttt ttcctgaatc acttttccgc ccaattgctt gactttgttt
     3601 agagtttgct gaatatcatc cactttaaaa taaattaaaa ctccttttcc ctcctgggtt
     3661 ttccataagc cgccaaacgg ctcctgtgat gtctttatgg tctcataagt cccttctgtt
     3721 ccccaagagc tcatctccca gttgaaaagc tgggtgtaaa aagattttgc cttttttaga
     3781 ttggaagtac aaatctcaaa atggcacagg aaatttgaca tctttacctc ctaaggattg
     3841 aattatgatt tatgggaaaa ttaattcatt tatcgagaaa gtcaattgaa aaatggattc
     3901 ctgctctttt gttatcatac atgggtaaca ctttggtata atcttgaggg tagtgaatta
     3961 ccccaagtaa cccagaacat tttaaggagg tgttacccat gcccaagcac ttcttgtggt
     4021 caactatcaa aaccaggctc aactggtttg aaatggctaa ggagaagaac atcacctatg
     4081 cctgtaagta ctttgggatc tccagaaaga ccttctacaa gtggtatagg cgctaccaag
     4141 gaagtggcga gaatcccaaa agcctccaag accagtccag aaggccaaag acgcacccta
     4201 aactcacttc tccggaggta gcttccttga tcatcaaact cagaggtgag accaaatatg
     4261 gaccaagacg attgcagttc tatcttaaaa gagactatgc tatcagcctt agcctttgtg
     4321 gtatctggaa ggtactatcc cgagctggac tgattgctca ctacaaaaga cgaaggaaac
     4381 gctaccagtc ctatggaaaa tatgtgcgtt atcttggaca taaagtccag atggatacca
     4441 aacccttgcc ccgaaggaaa ggacagttcg attcaaggga ttatcaatat gtcgctaagg
     4501 atgtgtttac caagttacgc ttcatcaaag tatatcaaga gctttcggta aataataccg
     4561 tggatttcac ccggagagct atcttgttct ttccctttaa gataaaacgc atccagactg
     4621 accatgggac agagtttacc tatgatatgc taagtagtac gcgcatccat cctttagata
     4681 agctcttaaa ggagcaaaag atcaaacacg ttctctctcc ggttgctact cctcgctaca
     4741 atggtcaggt ggaaagatcc atcaggactg acttagaaga gttctatcgc cctttaggtg
     4801 ttgtcaaaga tcatgctact ctatccaaga aacttctttc ctatctggag tattataatc
     4861 aatctcgtcc tcatatgacc ttgaatatgc tcactccgtt agagaaacta cattccactc
     4921 ctggatatga gaatgccaaa cttaactgga ggtgttacct atgattgaca acaatacaaa
     4981 aatggattcc tgcttgcgat atgaatcata cggctcgtaa aggacagaac aatgttctgt
     5041 ccctacaact agactgcctg ctgcccccaa atacaaacaa ttataactta actatatttt
     5101 ttaatttttc attggagaga accagtctca acaggggata acccagaacg tagcaggcga
     5161 tgacctcacc tacggcgatg gacaaaacgc aaattagata agatagctgg aaaagtttat
     5221 gcaggtagat gctcacgcca aaggcattga ttaaaaccgg tggcagggga gcgaggatag
     5281 gtttttttgt tttcgataca aaataagtca agaacgcagc cagcagggtg aacaagctgc
     5341 caaagatgat atctattatt cctgcggggc tgacaagatt ggctgcaata cagccaataa
     5401 aaagtccggg tatggcggct ggagtaatat atggcaatac agttaaaact tcagccaccc
     5461 ttatctgcat tggattataa ctaataggtg aaaagaccag aatggtcaag gccgcgtata
     5521 gagcagcaat gatggatgcc tgggcgatga atttattgga aaatttcata aattaccttt
     5581 tgataacctc accccttcgc ttcgctcagg gcagactcta accctctcct taaaaaggag
     5641 agggaattca aagattcgta attaccaaaa aacacttgtt ctgtcattac gagaagtcct
     5701 aaagctttcg tcgaagggcg acgaagcaat ctcccacttg tcattctgaa cggagccctg
     5761 caccgttatg gtgcaaggcg gagtgaagaa tctcctcaaa gtgagatcct tcccccgcta
     5821 aggcggggtc aggatgacgc ttgcgcttta ggttgtttcg tcccctccct tcgacttcgc
     5881 tcagggacgg tgagcagttc gaccctgagc tcactgccga agggcaaaga cgaaccgtat
     5941 ggggactcgc cctgaaccag aacggttcgg gactcgcaat gacagttaag agtatattta
     6001 aaaggcaaag ccacttcaca ttaaaaagta ttcaaaacga tttgtcaata aaaaaacctt
     6061 gtcagagtaa ttgggcaggt atatattaaa taattcagga gtaagttttt aacctcaccc
     6121 ttgccctctc cttgaaaagg agaggggaga gaaggggttt ataaaaggag atagtatgag
     6181 agttctggtt gtgggtagcg gaggaagaga gcatgctctg gcctggaaac tttcccaaag
     6241 ccctttaatt aataagatct atgctgcacc gggaaatgga gggctctcca ttttggcgga
     6301 gtgcgttgac atcgcagtgg atgacctgga gaagctggtg gatttcgctc acaagaacaa
     6361 tatcgatttg actgtagttg gtccggaact tcctttggtt ttgggcatag tggacctctt
     6421 ccaacagaag ggacttaaga tatttggacc aaccaagaag gcggcgcaga ttgagggaag
     6481 taagagtttt gccaaagaat tcatgagaaa gtatcatata cccaccggct cctttagaat
     6541 cttcacaaat aaagaagaag cttctgattt cgtcaattcc gccacttatc ctttggttat
     6601 caaagcagat ggattagctg caggcaaggg agtcatagtg gctgaagaaa aaaattccgc
     6661 cagggatgcg attgaaaaga taatggtaga taagatattc gccgaagccg gggcgaagct
     6721 ggtcattgaa gagttcctgg tgggagaaga ggtaacggtc ttggctttca cggatggcaa
     6781 gaatattgtt cctatggttc cttcacgtga ccacaagaga gtttatgacg gagatattgg
     6841 tcccaacact ggcggaatgg gcgcagttgc tcccaccacc atagttagtg agagaaagac
     6901 acaggagatt tatgatgaga tattggagcc aaccatttcc ggcttggaga gagaagggtg
     6961 ttcattcaag ggtatacttt atgcgggctt gatgatgacc gaggtgggac caaaagttct
     7021 ggaatacaac tgtcgtttcg gagatccgga aactcaggtg gtattaccct tactgaagaa
     7081 tgatttggcg gagatactcc tttccataat tgatggagac ctggatttgg agaagatcga
     7141 atgggagcag aagtttgcag tttgcgtggc cctggcttcg ggtggatacc cggacaaata
     7201 tgagaagaat atagagatat ttggcctgaa caaattggat aaagagaaaa gcagagacct
     7261 tcagattttc catgccggca ccagaaggtc ggttgacaga ttcttcacta ccgggggaag
     7321 ggttttggga attacctgcg tggatgagag tatggagaaa gcaatcaaga gatgttatgc
     7381 ggcgattgaa tatataaaat tcaacgggat gcattttcga aaagacatcg gatataaagt
     7441 gtcggggttg aggaaagagt attattgaag tagtttaaaa atttaacctc accctagccc
     7501 tctccttggc atgaaccatc agcgttcgcg cctcggaaag gagaggggat gtagttgggc
     7561 aaggtgccca acctacgatg aaaataacaa ctttcgtagt gcagggcatt tagccctgca
     7621 cggtgtaggg taaatttcta tccctacggt ataaaccata tgtttcatat ttatggcagg
     7681 gcaacttaga agcttaccct gaaccgttat ggttcagggc ttagcagttt gaaatttcaa
     7741 gaaattcgta ggggctcaat ttgcgcagca tcttgacgat atcgagtccg tgttactagt
     7801 ggcagatgcc accgggttac ccatataaag ctaaagcttt atactccaat gaatcggtta
     7861 aaaggagttt agagatgatg aaacctaaag ttctgattat cgttggcagc gagtcagatt
     7921 tggagataat gtccaaatgc gaggagatgc tggctaagtt tggcatttct tacaagctgg
     7981 aaatatcctc ggctcatcga aatccgcaaa aaacaataca gctcgccaag acagctgaaa
     8041 acaaaggata tagccttatt atctgtggag cagggatggc ggctcatttg ccgggagtgg
     8101 ttgcctccca tac
//
LOCUS       DASWLN010000012         2148 bp    DNA     linear   ENV 20-FEB-2024
DEFINITION  MAG TPA_asm: Candidatus Zixiibacteriota bacterium isolate
            SMAG_U2949 k141_394379, whole genome shotgun sequence.
ACCESSION   DASWLN010000012 DASWLN010000000
VERSION     DASWLN010000012.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37960594
            Sequence Read Archive: SRR10376091
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Candidatus Zixiibacteriota bacterium (soil metagenome)
  ORGANISM  Candidatus Zixiibacteriota bacterium
            Bacteria.
REFERENCE   1  (bases 1 to 2148)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 2148)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (26-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/12/2024 08:28:26
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 1,440
            CDSs (total)                      :: 1,416
            Genes (coding)                    :: 1,412
            CDSs (with protein)               :: 1,412
            Genes (RNA)                       :: 24
            rRNAs                             :: 1 (16S)
            partial rRNAs                     :: 1 (16S)
            tRNAs                             :: 21
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 4
            CDSs (without protein)            :: 4
            Pseudo Genes (ambiguous residues) :: 0 of 4
            Pseudo Genes (frameshifted)       :: 0 of 4
            Pseudo Genes (incomplete)         :: 3 of 4
            Pseudo Genes (internal stop)      :: 1 of 4
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2148
                     /organism="Candidatus Zixiibacteriota bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_394379"
                     /isolate="SMAG_U2949"
                     /isolation_source="soil"
                     /db_xref="taxon:2053527"
                     /environmental_sample
                     /country="USA: Oregon"
                     /lat_lon="42.99 N 123.63 W"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            complement(<1..880)
                     /locus_tag="VGB16_00310"
     CDS             complement(<1..880)
                     /locus_tag="VGB16_00310"
                     /inference="COORDINATES: protein motif:HMM:NF014790.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016627 - oxidoreductase activity, acting
                     on the CH-CH group of donors [Evidence IEA];
                     GO_function: GO:0050660 - flavin adenine dinucleotide
                     binding [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="acyl-CoA dehydrogenase family protein"
                     /protein_id="HEX9916156.1"
                     /translation="MDFELSEEQRMFKDAAAEFAKEKLFPKAQEFEEKEEFPPELLKE
                     LANLGYFGMLLPEQYGGSGLDFVSYACTLEEFCKGCAALGVLLSVHNSLVCGAILQYG
                     NEEQKKKYLPKLAKGEVLGAYSLTEPQAGTDAGKLKATAVLKDSNYVINGTKSFVSNG
                     KISNLIVLFCLTNPPAGSRGISALLVEKGAEGLSIGSKEKKMGLRASDTTELSFEDCK
                     IPKENLLGKENEGFKIALSLLDNGRIGIAAQALGIAQAALDEALRYSKERVQFGQAIC
                     NFQAIQFKLADMATQIE"
     gene            complement(922..>2148)
                     /locus_tag="VGB16_00315"
     CDS             complement(922..>2148)
                     /locus_tag="VGB16_00315"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_013925443.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="3-ketoacyl-CoA thiolase"
                     /protein_id="HEX9916157.1"
                     /translation="IYLTAGFNTISFGTGRKEFHPKKERPGLEYYITEAGKGVIAQIN
                     DPNHIDEGIISNFMAARFNKQGNLAAMMPMIHPSLEYKPHTRVEGACGSGGLGLLTSL
                     KSICSDISDVALAVGVEVQNTYKAIYGADILAGAAHYLTQRKNGHVYFFPDQFSQRAG
                     ACYDKFGYDYVRKGMATWYKIAIENARKNPKAQEYHNTIEDLMAAGMTPPNPKAFVDH
                     LNVYDCSKVSDGASAIIFASEDGLKKIGIDKKDAVEVVGFGTAVADISKYPEDKTRMT
                     TTQKAVQKAFDMAGIGPKNLGVLEVHDCFTVGGILALEAVGIAKYGEAAGFVAEGKTN
                     TNSEMPTNPTGGLVGFGHYTGGTGVRQAVDLLHQLTGKAGDCQVKIKPNRPYGMLINM
                     GGNDRTVTAMIFKRAE"
BASE COUNT          518 a          556 c          432 g          642 t
ORIGIN      
        1 actctatctg cgttgccata tctgccagct tgaattgaat ggcttgaaaa ttacatatag
       61 cttggccgaa ttgaaccctt tcctttgaat atctcaatgc ttcatctaaa gccgcctgcg
      121 caattcccag agcttgggct gcaatgccaa tccgaccgtt gtctaatagc gacaaagcta
      181 tcttgaatcc ctcgttttcc ttgcccagaa ggttctcttt gggaatctta caatcttcaa
      241 acgatagctc ggttgtgtcg gatgctctca gccccatctt tttttccttt gagccaatgg
      301 aaagtccttc tgcacccttt tcgaccaaga gggctgaaat ccccctggag ccagctggtg
      361 gattggtcag gcagaaaagg acaatcaaat tcgaaatctt accattgctc acaaacgatt
      421 tcgtgccatt tatgacgtag ttagagtcct taagcactgc agtagctttc aacttgccgg
      481 catctgttcc agcttgtggt tcagtcagag aataggcacc taatacttct cccttagcta
      541 atttgggaag atattttttc ttctgctctt cgtttccgta ttgcaggata gcaccgcaaa
      601 ccaacgaatt gtgcactgaa agcaagaccc ccaaagcggc gcaacctttg caaaattcct
      661 ccaaagtgca ggcataactg acgaagtcca aacccgagcc accatactgc tccggaagga
      721 gcatgccgaa ataccccaag ttagccaact ctttgagcaa ttccggcggg aactcctctt
      781 tctcttcgaa ctcctgtgct ttaggaaaaa gcttttcctt ggcgaactca gctgccgcat
      841 ctttgaacat tcgctgttct tcgcttaact caaaatccat tgtgcctcct gatttctatt
      901 ggttatcccc taaattaagg attattcagc ccttttgaat atcattgcgg ttacagttct
      961 atcatttcca cccatattga taagcatgcc gtaaggccta ttgggtttta tctttacctg
     1021 acaatctcct gcttttccgg ttaattgatg gagcaaatcg accgcctgcc tcactccggt
     1081 tcctccggtg taatgaccga atccaactaa tccgccagtg ggattggttg gcatttcgga
     1141 attcgtattc gtttttcctt ctgccacaaa accagctgct tcaccgtatt tggctatacc
     1201 caccgcctcc aaagccagaa ttccaccaac ggtgaaacaa tcgtgaacct ctaatactcc
     1261 aaggttcttg ggaccgattc ctgccatatc aaatgctttt tgaactgcct tttgagttgt
     1321 ggtcattctg gttttatcct ctgggtattt tgagatgtcc gccactgctg tgccgaagcc
     1381 cacaacctcg actgcatctt ttttgtcgat tcctattttc ttcagaccat cttctgaagc
     1441 aaaaatgata gccgacgctc cgtccgaaac cttggagcaa tcatagacat taaggtggtc
     1501 aacgaaggct ttgggattgg gaggggtcat tccagctgcc attaaatcct ctatggtgtt
     1561 gtgatattcc tgcgctttgg gatttttccg cgcattctct atggcaattt tgtaccaagt
     1621 tgccatacct ttcctcacat aatcgtaccc aaacttatca tagcatgccc cggctcgttg
     1681 agaaaattgg tccgggaaaa aataaacgtg cccatttttt cgctgggtaa gataatgtgc
     1741 cgcgccagcc agaatatccg caccgtaaat cgccttgtag gtgttctgaa cttctactcc
     1801 cacggctaag gcaacatctg aaatatcaga gcagatactt ttgagcgaag tcagaagtcc
     1861 taaaccgccg gacccgcaag caccttcaac tctggtgtgg ggcttgtatt ccaacgaagg
     1921 atgaatcatc ggcatcatcg ctgccagatt tccctgtttg ttgaatctgg ctgccataaa
     1981 gttggatatt atcccctcgt caatatgatt ggggtcattt atttgagcga ttactccttt
     2041 tcctgcttcg gttatataat attccaaacc aggtctctct tttttgggat gaaactcctt
     2101 cctgcctgtg ccaaaggaga tggtattaaa tcctgcggtc aggtaaat
//
LOCUS       DASWLN010000013         2764 bp    DNA     linear   ENV 20-FEB-2024
DEFINITION  MAG TPA_asm: Candidatus Zixiibacteriota bacterium isolate
            SMAG_U2949 k141_521048, whole genome shotgun sequence.
ACCESSION   DASWLN010000013 DASWLN010000000
VERSION     DASWLN010000013.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37960594
            Sequence Read Archive: SRR10376091
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Candidatus Zixiibacteriota bacterium (soil metagenome)
  ORGANISM  Candidatus Zixiibacteriota bacterium
            Bacteria.
REFERENCE   1  (bases 1 to 2764)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 2764)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (26-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/12/2024 08:28:26
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 1,440
            CDSs (total)                      :: 1,416
            Genes (coding)                    :: 1,412
            CDSs (with protein)               :: 1,412
            Genes (RNA)                       :: 24
            rRNAs                             :: 1 (16S)
            partial rRNAs                     :: 1 (16S)
            tRNAs                             :: 21
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 4
            CDSs (without protein)            :: 4
            Pseudo Genes (ambiguous residues) :: 0 of 4
            Pseudo Genes (frameshifted)       :: 0 of 4
            Pseudo Genes (incomplete)         :: 3 of 4
            Pseudo Genes (internal stop)      :: 1 of 4
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2764
                     /organism="Candidatus Zixiibacteriota bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_521048"
                     /isolate="SMAG_U2949"
                     /isolation_source="soil"
                     /db_xref="taxon:2053527"
                     /environmental_sample
                     /country="USA: Oregon"
                     /lat_lon="42.99 N 123.63 W"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            96..1214
                     /locus_tag="VGB16_00320"
     CDS             96..1214
                     /locus_tag="VGB16_00320"
                     /EC_number="3.1.-.-"
                     /inference="COORDINATES: protein motif:HMM:NF024258.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003677 - DNA binding [Evidence IEA];
                     GO_function: GO:0042578 - phosphoric ester hydrolase
                     activity [Evidence IEA];
                     GO_function: GO:0046872 - metal ion binding [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="DNA repair exonuclease"
                     /protein_id="HEX9916158.1"
                     /translation="MLRLLHTADIHLGARFPGLGRAGDRLRVITKSTFSRIVDLALEK
                     QVNLVLVAGDLFDSNHVSRTTVDFAINELSRLGEIPVCILPGTHDCYDISSVYKNMEQ
                     QDIPKNIILFTDSRKPYKLLENLDLTVYGKPNTSSSGRERPLANLVKMEGLTKYYIAM
                     AHGSVLVPSKSGEDEFPIRLKDIESSNFDYVALGHWHSFYEVSQGRTKAFYCGSPEQI
                     KFGSKDAGSVILVELAENINVEKIRVGSTVWKEIEFSTEKLKSQLELNKEIEQYQGEM
                     AILKARISGVSPTDSDLNVSKVVQELRDKFLHLAIEDNTVTVPEDFVNLKLPKNTILG
                     QFVEIMEEKIKTSRPQEKEKVEQALKTGYALLTGKEVF"
     gene            1231..>2764
                     /locus_tag="VGB16_00325"
     CDS             1231..>2764
                     /locus_tag="VGB16_00325"
                     /inference="COORDINATES: protein motif:HMM:NF024867.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016887 - ATP hydrolysis activity
                     [Evidence IEA];
                     GO_process: GO:0006302 - double-strand break repair
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="AAA family ATPase"
                     /protein_id="HEX9916159.1"
                     /translation="MKLDKFIVKRFRKLSDFKGNFTDGINLIKGPNEAGKSTLVDAIT
                     CAFFEDPKSTKKGLKSKTSWGMDKGFEVQVDFTANGSAYTLKKDFESGEIKLTNKSTN
                     EKWDDKKRVEELIFREMGFPSEEIFLSTACIQQDEMSRFASNADAIKDRLEALVTGGK
                     ELIRASEVLDKLNLRISQLKKEGTKNLGEIQTYERVRDELMEELDKLRRGNSAVFENR
                     ASLVEISSSLEKWNKEHQFKKGRLEKAKEAIQLYEKIKGVEGKHNELTERVKKVQGSE
                     SNVRKLREESSRLPSIDRSDMANLEELEVKVKYLEGKRSDAEMEQQELKKDLALNKRK
                     AKFVWMVPVFFLLTVAGIVMGYLKWELYFWVAGMFAVVLLTFLIPSVIYVNRKNSLKK
                     QLGLRKSRLEEIEADIYHANLALNSMMEKYKAPNMYDLREHFDNLRDIERQIKSEMSK
                     YEGLLGDKNLKELEEELNALTLEYTGYQERFEKVRLYNMSVEELDRLSQEMAEVEEEK
                     KKL"
BASE COUNT          887 a          471 c          725 g          681 t
ORIGIN      
        1 cgcttcaatt ttatctaaaa gaaagagcca attcaaccga taatagaaag ggttagtgta
       61 agtagaggca tgctcgaaat acgtggggat ggactatgct tagattacta cacactgccg
      121 atattcacct cggagccaga ttccctggat tgggaagagc cggggataga ctgagagtga
      181 ttacaaagag cactttctct cgcatcgtgg atttggctct ggaaaagcag gtgaatctag
      241 tgctggtggc tggggatcta tttgactcca atcatgtttc cagaacaact gtagatttcg
      301 caataaatga gctttcccgt ctgggggaaa tcccagtttg tattcttcct ggaactcacg
      361 actgctatga tatcagttca gtttataaga atatggaaca gcaagatatt cccaaaaaca
      421 tcatattatt cacagatagc cgtaagcctt ataagttgct tgaaaacctt gatttgacag
      481 tctatggcaa acctaatacc tcctccagcg gtcgcgaaag acctttggct aacttggtca
      541 agatggaggg tcttaccaaa tattacatcg ctatggcaca tggctcggtt ttagtacctt
      601 ccaaaagcgg ggaggatgaa ttccccattc gattgaaaga tattgagtcc tctaatttcg
      661 actacgtagc attgggccat tggcattcgt tttatgaggt ctcccaggga aggaccaagg
      721 ctttctactg cggttcccca gagcagatta aatttggctc caaggatgcg gggagcgtga
      781 tcttggtgga attggcagaa aatattaatg tggagaagat acgtgtcggt tcaaccgtgt
      841 ggaaagagat agagttttcc actgagaagc tcaaatcgca gcttgaacta aataaggaga
      901 tagagcaata tcaaggtgag atggctattc tcaaagcgag gataagcggc gtttctccca
      961 ctgactctga tttgaatgtc tccaaagtcg tgcaagaact acgagataaa tttcttcact
     1021 tagcaattga agataatacg gtgactgtgc ctgaggattt cgtgaatctg aagctaccca
     1081 agaataccat cttaggccaa tttgtagaga taatggaaga gaagataaag acctcccgcc
     1141 ctcaggaaaa ggaaaaagta gagcaagccc tgaaaacagg atatgctttg ctaaccggca
     1201 aagaggtttt ttgaagaagg gacaaggtag atgaagctcg acaaattcat cgtaaagaga
     1261 ttcaggaaat tatctgattt caaagggaac ttcaccgacg ggataaattt gattaaggga
     1321 ccaaatgagg cgggcaagtc cactttggta gatgccataa cttgtgcatt cttcgaggat
     1381 cctaaatcca ctaagaaggg actcaaaagc aagaccagct ggggtatgga taaagggttt
     1441 gaagtgcagg ttgattttac agccaatggt tcagcctaca ctctcaagaa agattttgaa
     1501 agtggagaga ttaagttgac caataaatcc accaacgaga aatgggacga caaaaagaga
     1561 gtagaggagc tcatttttcg agaaatgggc tttccctccg aagagatatt tttatccacg
     1621 gcctgcatcc agcaagatga gatgtctcgt tttgccagca atgctgatgc cataaaggat
     1681 agattggaag cgctggttac cggcgggaaa gagttaatac gcgcatccga agtgttggac
     1741 aagttgaatc taaggataag tcagctgaag aaagaaggga ctaagaactt gggcgagatt
     1801 caaacctacg agagagtaag ggatgagcta atggaggaat tggacaaact gagaaggggc
     1861 aacagcgctg tttttgagaa tcgagcgagc ttggtggaaa tcagttcaag cctggagaag
     1921 tggaataaag agcatcaatt caagaagggg cgtttagaga aagccaagga agcgatccag
     1981 ctgtatgaga agataaaggg agtggagggt aagcataatg aactgactga acgggtaaag
     2041 aaagtgcaag ggtctgagag caatgtgagg aaattgcgag aggagtcctc caggttacca
     2101 agtatagata gaagcgatat ggctaacttg gaagagttgg aggttaaggt caagtatttg
     2161 gaggggaaaa gatcagatgc cgagatggaa caacaggagc tgaaaaagga tctggctctt
     2221 aacaagagaa aagctaagtt tgtgtggatg gtgccggtct tcttcttgct gacagtcgca
     2281 ggtattgtca tggggtatct caagtgggaa ttatactttt gggttgcggg tatgtttgct
     2341 gttgtattac ttacattcct aattccatct gtcatctacg ttaatcgcaa aaattctctc
     2401 aagaagcaac ttggattaag aaaatcaagg ttagaggaga tagaggcaga tatctatcat
     2461 gctaacttgg cactcaattc tatgatggag aaatacaaag ctccgaatat gtatgatcta
     2521 agagaacatt ttgataacct gagggacatt gaacggcaga tcaaaagtga gatgtctaaa
     2581 tatgaaggtc tcttgggtga taaaaattta aaggaattgg aggaggagct taatgctctc
     2641 accctggagt atactggata tcaggagaga ttcgaaaaag tcaggttata caatatgagc
     2701 gtggaggaac tggataggct tagccaggag atggcagagg tggaggaaga aaaaaagaaa
     2761 ttag
//
LOCUS       DASWLN010000014         7473 bp    DNA     linear   ENV 20-FEB-2024
DEFINITION  MAG TPA_asm: Candidatus Zixiibacteriota bacterium isolate
            SMAG_U2949 k141_768260, whole genome shotgun sequence.
ACCESSION   DASWLN010000014 DASWLN010000000
VERSION     DASWLN010000014.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37960594
            Sequence Read Archive: SRR10376091
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Candidatus Zixiibacteriota bacterium (soil metagenome)
  ORGANISM  Candidatus Zixiibacteriota bacterium
            Bacteria.
REFERENCE   1  (bases 1 to 7473)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 7473)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (26-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/12/2024 08:28:26
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 1,440
            CDSs (total)                      :: 1,416
            Genes (coding)                    :: 1,412
            CDSs (with protein)               :: 1,412
            Genes (RNA)                       :: 24
            rRNAs                             :: 1 (16S)
            partial rRNAs                     :: 1 (16S)
            tRNAs                             :: 21
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 4
            CDSs (without protein)            :: 4
            Pseudo Genes (ambiguous residues) :: 0 of 4
            Pseudo Genes (frameshifted)       :: 0 of 4
            Pseudo Genes (incomplete)         :: 3 of 4
            Pseudo Genes (internal stop)      :: 1 of 4
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..7473
                     /organism="Candidatus Zixiibacteriota bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_768260"
                     /isolate="SMAG_U2949"
                     /isolation_source="soil"
                     /db_xref="taxon:2053527"
                     /environmental_sample
                     /country="USA: Oregon"
                     /lat_lon="42.99 N 123.63 W"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            complement(<1..1005)
                     /gene="waaF"
                     /locus_tag="VGB16_00330"
     CDS             complement(<1..1005)
                     /gene="waaF"
                     /locus_tag="VGB16_00330"
                     /inference="COORDINATES: protein motif:HMM:TIGR02195.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008920 - lipopolysaccharide
                     heptosyltransferase activity [Evidence IEA];
                     GO_process: GO:0009244 - lipopolysaccharide core region
                     biosynthetic process [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="lipopolysaccharide heptosyltransferase II"
                     /protein_id="HEX9916160.1"
                     /translation="MKNKPKKILIIQTAFLGDVILTTPLVKATRELYPQAFISILLVP
                     QTAEVFENNPGINQIILYDKKGKQKGIRSFFRLIRFLEKEKFDLALLPHRSLRSALLA
                     YLSGIPQRIGFHTSSSSFLYTSEVTYRSGLHEIDRVLSLLTPLGHNVHNIKPELFPKD
                     EDLIWADRFLNECGIDKNKKIVGIAPSSVWATKRWPLERYACVAERLTNGSNVEVLLL
                     GSNTDERLLLKISSLMNRKPIIPIGQSILLKSVALMSRCSVVLSNDSAPVHMATALEK
                     PTVAIFGPTLPEFGFAPYGEGHTVIQRELYCRPCGIHGRKSCPEIHFRCMRDISPDEV
                     F"
     gene            complement(995..2452)
                     /locus_tag="VGB16_00335"
     CDS             complement(995..2452)
                     /locus_tag="VGB16_00335"
                     /inference="COORDINATES: protein motif:HMM:NF014385.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0015562 - efflux transmembrane transporter
                     activity [Evidence IEA];
                     GO_process: GO:0055085 - transmembrane transport [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="TolC family protein"
                     /protein_id="HEX9916161.1"
                     /translation="MMKTTLIFGVILVLFMSMQQTKADKVLSLNLDDCLKTVIRNSSE
                     YKIAQMRYRAAEASYSSAKLSYYVPNLTLNFNTPEYSWFGDYQDYYGAAGRVYFVEEY
                     YNYTSSLDLSQKLFTGGDVTVSGLLNSYRRTSNIFTNYDEVRTNLQFSIEQPIFNWNR
                     HKIELDKARVDYDLSRLKYSEETNQVISDLTTNYIHYLTSQKQLKIAELKVQNADLDY
                     ISAEKKSQEGLISDMEFAQKRTEKMDRKLELLDGKRNFEEYQAKLLQMLKIEEEVELN
                     LNQNIELGQFSLEQEKNKNIGQSSEVLKAQGEARKKELELKEAKASGGIDGRVNFYYG
                     FLGRGPIARESIDDFKKNRWGVSIVLSLPIWDGGAHSFSIRSSQLSLEEAKKASELTK
                     SQMKSKLEAIVSKLSTAWEKVGFLKEELKLAGDDWTNAQKKNQMGLLSEGQMLDAQIT
                     FLETEVKYLNAVLDYRIYRIELDKMWGMVPESDEK"
     gene            complement(2627..3895)
                     /locus_tag="VGB16_00340"
     CDS             complement(2627..3895)
                     /locus_tag="VGB16_00340"
                     /inference="COORDINATES: protein motif:HMM:NF024116.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ABC transporter permease"
                     /protein_id="HEX9916162.1"
                     /translation="MRFRSTFSLSFDAIQAHKFRSFLTTLGVMFGVAAVIAMLSIGEG
                     AKRDALEQIEMMGTSNIIIKAKKPPKTKTGESQLNLSEGLNLADAENIKHFSSLVENV
                     VPQVSFKNVDDIQYKDRITKGTIIATTPEYKNLMHMKLVNGRFFTVKENEEAKQVCVL
                     GYNMKKELFAFSNAIGKSIKTGVGYYFTVIGVLEYKKTGKSKIEGVEVRDINEEIYIP
                     LEFMLKKIGKYPLTDEKGEDFQGVLSEIAVQVKKGRGIAEVAGLVQKVLERRHRGVED
                     YKIIIPEALLRQSQRTQRIFNIVMGCIAGISLLVGGIGIMNIMLATVLERIREIGVRR
                     AVGARKIDVMQQFLLEAVAISFVGCILGVILGFVLAKGINFYAQWRTIISFGSVLLAV
                     GVSAAVGIGFGYYPARKAATLDPIEALRYE"
     gene            complement(3895..5106)
                     /locus_tag="VGB16_00345"
     CDS             complement(3895..5106)
                     /locus_tag="VGB16_00345"
                     /inference="COORDINATES: protein motif:HMM:NF027890.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005215 - transporter activity [Evidence
                     IEA];
                     GO_process: GO:0006810 - transport [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="efflux RND transporter periplasmic adaptor
                     subunit"
                     /protein_id="HEX9916163.1"
                     /translation="MDKTIKIIRIVIYIIAALIIIYLVKRVFFTKLDIPAAEAHKGEF
                     VISLTEKGELDAKKSISISSPRVRGNLLITKLIPEGTIVEKGDTIMEFDKLELQRNLV
                     DYQANLKIAQANLLKAQGESDLTKKQLQLELDQAEREAKEKQFEAPLIKQEAEKKLEL
                     ARLKYETNIKSMESDIQKLKIEVDKARYEVERAQDYLSQLTVTAPIPGLVVYLEYWKG
                     GQWGKVQEGDSPWPGQGLINLPDLSMMLTKARVHEVDIDKVKLGQNVNIILESYPETT
                     FTGKVDKIARLATRDAEDNSNINTFDIEIMIDRTDERLKPGMSAKCEIIVDKIPNSIW
                     VPIEAVFEKDNKTVVYVIKGNNFRERQIKVGQRNDTDIIVTEGLKGKEKVALRDPTSK
                     EEAGAIKAKEK"
     gene            complement(5416..5865)
                     /locus_tag="VGB16_00350"
     CDS             complement(5416..5865)
                     /locus_tag="VGB16_00350"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEX9916164.1"
                     /translation="MKKFWRWLLASVVVIIFSLAVQVSRMIIFAEHGYYYATPDIFLG
                     LIFPLFYVILYQGIPGKGIIKGLVLGMLCWLLFFLPHNAKGIEFSFSLDAHLATFGAG
                     SSLSNLSSFNVVKAISYLLGCSLGGIVIAFIFGKSLEVEKANAGTGV"
     gene            complement(5882..6784)
                     /locus_tag="VGB16_00355"
     CDS             complement(5882..6784)
                     /locus_tag="VGB16_00355"
                     /inference="COORDINATES: protein motif:HMM:NF015251.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="lysophospholipid acyltransferase family protein"
                     /protein_id="HEX9916165.1"
                     /translation="MAQRKLSHYLEYIITRTFGFVLNLLPYSWAMRFGDFLGWVSYRI
                     LHIRRKITLTNMKAAFKDAYSPEELDKIGLRSYQIIAKSFIDYILFPSLKNKVLEMIE
                     FEGKENFDKALKKGKGAILVTGHFGSWELMGAAIAKSGYATDFLVGEQHNTLIDDVMN
                     DYRQLMGVGIIKMGVAAKGVIKALKNNRFVAMLSDQDAGGDGTIVSFFGMPASTPKGP
                     AAFAIKTGAAILTGGIIRDKSGRQKIKVEEPIYLETSGNKEEDIQKLTQIYTSILENY
                     IRKYPDHWFWPHRRWKSTVKELYK"
     gene            complement(6766..>7473)
                     /locus_tag="VGB16_00360"
     CDS             complement(6766..>7473)
                     /locus_tag="VGB16_00360"
                     /inference="COORDINATES: protein motif:HMM:NF024876.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="peptidase MA family metallohydrolase"
                     /protein_id="HEX9916166.1"
                     /translation="TPQAMVFVTQNDKEFDSLVGGSFPDWGIAAAVPEQNLIVLKSPI
                     KYGYSQDLPRVLMHELAHLYLGNKVNRQNFPRWLDEGFAMYMAEEWRWSLDLSIAKAV
                     FTNSILNLVVIDSVNSFNSPKAHLAYLESFLAINYFIQQYGEDNFKQLINLLAGGKDI
                     NSALVQTIGLNHLQFSDEFSQYLKKKYSWISFFSDTFLLWLLLAFLVITFYFMKKMRS
                     RKIMQDWEKEESWPKEN"
BASE COUNT         1956 a         1828 c         1369 g         2320 t
ORIGIN      
        1 gaagacctca tctggggata tatcccgcat acagcgaaaa tgaatctccg gacaagattt
       61 tctaccgtga attccgcagg ggcgacaata aagctctctt tgaataacag tatgaccttc
      121 tccatatgga gcaaatccaa actccggcaa ggtcggaccg aaaattgcca cagtcggctt
      181 ttctaaggca gttgccatat gcactggtgc gctatcgtta gacagcacga ctgagcatct
      241 tgacatcaga gcaaccgatt tcaaaagaat agattggcca atcggaatga tgggttttct
      301 gttcattaat gaggagatct ttagaagtag tctttcgtca gtgtttgaac ccaaaagcaa
      361 gacctccacg ttggatccat ttgtcaatct ctctgccaca caagcatacc tttccagcgg
      421 ccaccttttg gttgcccaga ccgaagaggg agcgatccct acgatttttt tgtttttgtc
      481 tattccacat tcatttaaga acctatctgc ccaaatgaga tcctcatctt ttggaaaaag
      541 ctctggcttt atattatgaa cattatgtcc taaaggagta agcaggctca ggaccctatc
      601 tatctcatgc aggcccgacc tgtaggttac ttcgctcgta tagagaaaag agcttgagct
      661 agtatgaaac cctatcctct gaggaattcc ggaaagatat gccaagagcg cactgcgcaa
      721 agaacggtgt ggaagtagag ctaaatcgaa tttctctttt tcaagaaatc tgatcagcct
      781 gaagaaactt cttatcccct tctgttttcc cttcttatcg taaaggatga tttggtttat
      841 gcctggattg ttttcaaaga cttctgcggt ttgaggaaca agaaggattg aaataaaagc
      901 ttgaggataa agttctctcg tggctttgac cagaggggta gttaaaatca catcccctaa
      961 gaatgcggtc tgaatgatta gtattttttt gggtttattt ttcatcagat tccggaacca
     1021 tcccccacat tttatctaat tctatgcggt atatcctata atctaaaact gcattcagat
     1081 atttgacttc agtttccaaa aaagttatct gtgcatctaa catctgccct tcagaaagta
     1141 aacccatctg gtttttcttt tgggcattag tccagtcgtc tccggctaac ttcaactctt
     1201 cttttaaaaa gcctaccttt tcccaggccg tggagagctt gctcacaatg gcttcaagtt
     1261 tgcttttcat ctgacttttg gtaagctctg aggctttctt tgcttcttct aaggataatt
     1321 gagagcttct gattgagaag gaatgggcgc ccccatccca gatgggtaag cttaaaacaa
     1381 tggagactcc ccatcggttc ttcttaaaat cgtctatgct ctccctggca attggtcccc
     1441 tgcccagaaa tccatagtag aagttgaccc tgccatctat ccccccgctc gccttagctt
     1501 ccttgagctc caattccttt tttcttgcct ccccttgtgc ttttaagacc tctgaactct
     1561 gcccgatgtt cttatttttt tcctgctcca gcgaaaactg gcctaattct atattttgat
     1621 ttagattcaa ttccacttct tcttctattt tcagcatctg cagaagtttt gcctggtatt
     1681 cctcgaaatt ccttttacca tccaatagct ctaattttct atccatcttt tctgttctct
     1741 tttgagcgaa ctccatatcg cttatcaacc cctcttggct cttcttttca gccgaaatgt
     1801 aatccaggtc cgcattctga actttaagct cagctatctt tagctgtttc tgactggtga
     1861 gatagtgaat gtaattcgtg gttaggtcgc ttataacctg attggtctcc tctgagtact
     1921 tcagtctgga gagatcgtaa tccaccctgg ctttatccag ctctattttg tgtctattcc
     1981 aattgaagat tggctgctct atactgaatt gcagatttgt ccgaacctca tcgtagttgg
     2041 tgaagatgtt ggaggtgcga cgataagaat taagcagccc ggagacggtc acatctccac
     2101 ccgtgaagag cttttgagaa aggtcgagcg agctggtgta attataatac tcttcaacga
     2161 agtagactcg gcctgctgct ccatagtagt cttgataatc gccgaaccag gaatactccg
     2221 gagtattgaa atttagagtc aaatttggaa cgtagtaact cagtttagcg gaactgtaac
     2281 ttgcctccgc tgcccggtat ctcatctggg caatcttata ttcgctgctg ttccttatta
     2341 ccgtcttcag acaatcatcc aagttcaagg acagaacctt atctgctttg gtttgctgca
     2401 tcgacataaa cagaaccaaa atcactccaa aaattaaagt tgttttcatc atttttgtaa
     2461 accccttaga attcgttcga taaatcaaac aactacattc tcgaaactat gaatttaaaa
     2521 ctgtttttct gtagttgcca catttatggg gcgaatttgg aatttatgta gttgcccaat
     2581 ttattgggca gttttatatt tatttttcta aaaacatgaa accgctctac tcatacctta
     2641 acgcctcaat cgggtccagg gttgccgcct tcctggcagg gtaatagccg aacccaatcc
     2701 ccactgcggc tgaaacccca actgccagaa ggaccgagcc aaaagagatt atggttctcc
     2761 attgggcata gaaatttatt cctttagcca gaacgaaacc taaaattacc cctaagatgc
     2821 accccacaaa gctgattgct accgcttcaa gcaaaaactg ctgcataaca tcaatcttcc
     2881 tggcacccac tgcccgacgc acaccaatct ctctgattct ctccaaaacc gtagccaaca
     2941 ttatattcat tatcccgatt cccccaacca aaagggatat gccggcaatg catcccatca
     3001 cgatattgaa aatccgctgc gttctttggc tttgtcttag tagggcttcg ggaattatga
     3061 ttttataatc ctccactcct ctatgccttc gctctaatac cttttgcaca agcccagcca
     3121 cctccgctat accccttccc tttttcacct gcactgcaat ttcgctcagc acgccctgaa
     3181 aatcctcccc tttttcgtct gttaaagggt atttgccgat tttcttcaac atgaactcca
     3241 gggggatata tatctcttca tttatatccc ttacctccac tccctcgatc tttgattttc
     3301 ctgtcttttt atattccaga actccgataa ccgtaaaata ataacccaca cctgttttta
     3361 ttgacttgcc aatggcattg gaaaaggcga aaagctcctt cttcatattg tagcccaaaa
     3421 cgcaaacctg tttagcctct tcgttttcct ttacggtgaa gaatctaccg ttaacaagtt
     3481 tcatatgcat cagatttttg tactctggcg tggtggcgat aatcgtcccc ttagtaattc
     3541 tatctttgta ctgaatatca tccacattct tgaaggaaac ttgcggcacc acattttcta
     3601 ctaatgaaga aaaatgctta atattctctg catcagccag atttaatcct tcagatagat
     3661 taagctggct ttctccggtt ttggttttgg gtggtttctt tgctttgatg atgatattgc
     3721 ttgttcccat catttctatc tgctccaggg cgtcccgctt tgctccttct ccaatggaaa
     3781 gcatagcaat cactgccgcc actccaaaca tcacgcccag agtggttaga aacgaacgga
     3841 acttgtgggc ttgaatggcg tcgaaagaga gggaaaaggt gctgcgaaac cgcatcactt
     3901 ttcctttgcc ttgattgcgc cagcttcctc tttcgaggtt gggtcacgca gagcaacttt
     3961 ctctttccct ttcaatcctt cggtgactat tatatcggtg tcgtttctct gtcccacttt
     4021 aatttgtctt tccctgaagt tatttccttt tatcacatat accacagttt tgttatcttt
     4081 ctcaaatacc gcctctatcg gcacccagat tgagtttgga atcttatcca cgattatctc
     4141 gcatttggct gacatacctg gcttgagccg ttcatctgtt ctatctatca ttatctctat
     4201 atcaaaagta tttatgttcg agttgtcctc cgcatctctt gttgccaaac gggcaatctt
     4261 gtccacttta ccagtaaagg tagtttctgg atacgattct aagattatgt tcacattctg
     4321 ccccaacttg actttgtcaa tatccacctc atgcactctg gctttagtca gcatcattga
     4381 taggtcaggc agatttatca atccctgtcc gggccaaggg gagtcgcctt cctgcacctt
     4441 accccattgt cctcccttcc agtattccaa atagaccacc aacccgggaa taggtgcggt
     4501 cacagtcaat tgggaaaggt aatcttgtgc cctttccact tcatatcttg ctttatccac
     4561 ctcgatcttc agtttttgaa tatccgattc catcgacttg atgttggtct cgtatttcaa
     4621 tctggctaat tccagcttct tctccgcttc ctgtttaatc aaaggggctt caaactgctt
     4681 ctccttggct tccctttctg cctggtccaa ttccaattgt aattgctttt tggtgaggtc
     4741 actttcacct tgagccttta acaagttggc ttgggcaatc ttcagattgg cttgataatc
     4801 caccaagttc ctctgcaatt ccaacttgtc gaattccatt atggtatctc ctttttccac
     4861 gatagtccct tcaggaatta acttggtgat taaaagattt cccctcaccc tcggtgagct
     4921 gatggagatc gactttttgg catccagctc tcctttctcg gttaagctga taacaaattc
     4981 ccccttgtgg gcttcagctg caggaatgtc cagttttgtg aagaaaactc ttttgacgag
     5041 gtaaatgatt attaaagccg caatgatgta aataacgatg cgaataattt ttattgtttt
     5101 atccatagag tttaggtttt aggtttttgt agttatcaaa tattcttctg tcattcccgt
     5161 cccgccaaag gcgggaggaa tccagtcttg aaaaacaaat cgattcctgc acgaattctg
     5221 tgtcttgagg ctggagttta tcccctacgg gggcagcaat gacaacaaac aagttgtatc
     5281 gcgtaggggt ggagcttgtc tccacccgca aactaaaaca ttctttcggg cgaagacgcg
     5341 gttcggcagg ctcaccgtcc tgagcaaagt cgaaggaaac ttcgccccct acgcgcaata
     5401 ttctattgga caggtctaaa cacctgtccc tgcgtttgct ttctcaactt ccaaactttt
     5461 gccgaagata aaggcaataa cgattccgcc taatgaacaa cctaacaagt aactgatggc
     5521 tttaacaaca ttaaatgacg agagattact aagagaagag cctgctccga aagtcgccaa
     5581 atgagcgtca agactaaaag aaaactcgat accctttgca ttatgaggca agaaaaacag
     5641 tagccagcac aacatcccta agaccaaccc tttaatgatg ccctttcctg gaataccctg
     5701 gtagagtatc acataaaata aggggaaaat caatccaagg aatatatcag gagtcgcgta
     5761 atagtaaccg tgctcagcaa aaattatcat ccgggaaacc tgtacggcaa ggctaaaaat
     5821 gataactact acacttgcca acaaccatct ccaaaacttc ttcataaaac cccctttctg
     5881 tttatttata tagctccttc accgtacttt tccatctcct atgcggccaa aaccagtggt
     5941 caggatattt ccttatataa ttctccaaaa tcgaagtgta aatttgggtg agcttctgaa
     6001 tatcctcttc tttattaccc gatgtttcca gatatattgg ctcttccacc tttatcttct
     6061 gcctcccgct cttgtctctt atgatccctc ctgtaagtat tgcagcaccg gtcttgattg
     6121 caaatgcggc tggccccttg ggagttgaag ccggcatgcc aaagaagctc acaatcgttc
     6181 catctccacc cgcatcctgg tctgataaca ttgccacgaa tctgttattc tttaatgcct
     6241 ttatcactcc cttagccgcc acccccattt ttatgatgcc gactcccatt agctggcgat
     6301 aatcattcat cacatcatcg attaaggtat tgtgttgttc ccccaccaaa aaatcagttg
     6361 cataaccact tttggctatg gctgctccca taagctccca gctcccaaaa tgaccggtta
     6421 ccagaatggc tcccttacct ttttttaggg ctttatcgaa attttccttc ccttcgaatt
     6481 caatcatctc caagacttta tttttaaggg atgggaaaag gatgtaatca ataaaagatt
     6541 tggcaataat ttgataactt ctaagtccta tcttatccaa ttcttctgga ctatatgcat
     6601 ctttgaatgc cgctttcata ttggtcaggg ttatcttcct tcggatatga agaatccgat
     6661 aggaaaccca tcccagaaaa tctccaaacc tcattgccca agaatatggc aaaaggttta
     6721 aaacaaaacc aaatgtgcgg gtgataatat actcaagata atggcttaat tttctttggg
     6781 ccaactttct tccttttccc agtcttgcat tatttttctt gaccgcatct tcttcataaa
     6841 ataaaaggtt attactaaaa aagcaagtag caaccacaaa aggaaagtat cactgaagaa
     6901 acttatccag ctgtatttct tctttagata ctgtgagaat tcatctgaaa actgcagatg
     6961 atttaagcca atggtctgga ccaaggcgga attaatatct tttccaccgg ccagaaggtt
     7021 tatcaattgt ttgaaattat cctcaccata ctgctggatg aagtaattta ttgccaagaa
     7081 actttccaga taagccaaat gggctttggg ggagttgaag ctattgaccg agtcgatgac
     7141 cactaaattc aaaatcgaat tggtgaaaac cgctttggct attgataaat ccagactcca
     7201 tctccattcc tctgccatat acatagcgaa tccctcatcc agccagcgag gaaagttctg
     7261 acgattgacc ttattgccca gataaagatg agctaactca tgcatcagaa ctctgggcag
     7321 gtcttgtgaa taaccatact ttatgggcga ttttaaaacg ataagatttt gttccggaac
     7381 tgcggctgca atgccccaat ccggaaaact cccgccgacc agagaatcga actccttatc
     7441 attttgagtt acgaagacca tagcttgtgg agt
//
LOCUS       DASWLN010000015         5504 bp    DNA     linear   ENV 20-FEB-2024
DEFINITION  MAG TPA_asm: Candidatus Zixiibacteriota bacterium isolate
            SMAG_U2949 k141_649421, whole genome shotgun sequence.
ACCESSION   DASWLN010000015 DASWLN010000000
VERSION     DASWLN010000015.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37960594
            Sequence Read Archive: SRR10376091
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Candidatus Zixiibacteriota bacterium (soil metagenome)
  ORGANISM  Candidatus Zixiibacteriota bacterium
            Bacteria.
REFERENCE   1  (bases 1 to 5504)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 5504)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (26-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/12/2024 08:28:26
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 1,440
            CDSs (total)                      :: 1,416
            Genes (coding)                    :: 1,412
            CDSs (with protein)               :: 1,412
            Genes (RNA)                       :: 24
            rRNAs                             :: 1 (16S)
            partial rRNAs                     :: 1 (16S)
            tRNAs                             :: 21
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 4
            CDSs (without protein)            :: 4
            Pseudo Genes (ambiguous residues) :: 0 of 4
            Pseudo Genes (frameshifted)       :: 0 of 4
            Pseudo Genes (incomplete)         :: 3 of 4
            Pseudo Genes (internal stop)      :: 1 of 4
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..5504
                     /organism="Candidatus Zixiibacteriota bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_649421"
                     /isolate="SMAG_U2949"
                     /isolation_source="soil"
                     /db_xref="taxon:2053527"
                     /environmental_sample
                     /country="USA: Oregon"
                     /lat_lon="42.99 N 123.63 W"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            <1..507
                     /locus_tag="VGB16_00365"
     CDS             <1..507
                     /locus_tag="VGB16_00365"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_014559336.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016746 - acyltransferase activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="phosphate acyltransferase"
                     /protein_id="HEX9916167.1"
                     /translation="GTKDKVFIFGDCAVLPNPDVEQLASIAISSARTMKELIGEEPKV
                     AMLSFSTKGSAQHEMVDKVVEATRLVKQQVPDLQIDGELQLDAAIIPDVAKKKSPNGP
                     VAGKANVLIFPDLNAGNIGYKLTQRLAQAEAIGPIIQGLAKPANDLSRGCSVDDIVNV
                     VAIAMTLA"
     gene            799..1218
                     /locus_tag="VGB16_00370"
     CDS             799..1218
                     /locus_tag="VGB16_00370"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEX9916168.1"
                     /translation="MNNDFKRFFLAGIIQGSKKGRNLHNQDYRKKIRFLLTKYFPQAE
                     IIDPVKVHPNSAEYASTKGKRVFHRSIEQAKGCDCFIAYLPTASMGTAIEMWECKNKR
                     IPIWTISPLKENWSVKFLSSTVFKNMKEFEKFISTRP"
     gene            1380..2162
                     /locus_tag="VGB16_00375"
     CDS             1380..2162
                     /locus_tag="VGB16_00375"
                     /inference="COORDINATES: protein motif:HMM:NF012340.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008234 - cysteine-type peptidase activity
                     [Evidence IEA];
                     GO_process: GO:0006508 - proteolysis [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="C1 family peptidase"
                     /protein_id="HEX9916169.1"
                     /translation="MSRFGCIKDKFDDRDYLMRAYLPVVKRPKKVDYTQKMSPIRDQG
                     EEGTCVGFAVASGMKEYQESLDYEKLVELSPRFVYSEAKKIDGVPGLEGTTIRASMQV
                     LEKIGVCQEKLWPYSPHQKDKPKVGAKANAQNFRIITYARILNLNELRMALLSKGPCV
                     LGIEVFKGMLKTKTGIVPLPRKNETGLGGHAITACGYDDEKGLVKVKNSWSEKWGDKG
                     YGYLPYAYIERYMMDAWSSVDIEDPNPLTLASVLNYKKRASV"
     gene            2193..2459
                     /locus_tag="VGB16_00380"
     CDS             2193..2459
                     /locus_tag="VGB16_00380"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEX9916170.1"
                     /translation="MKQDDALIKLEEKENAILFKFGWMFRNSLNEFSTSVDRSLGYAA
                     KIVLPMIMASKEDKSYNPFAQIIEKYAIHILIHKLEKEGYKLLP"
     gene            2526..2825
                     /locus_tag="VGB16_00385"
     CDS             2526..2825
                     /locus_tag="VGB16_00385"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_008656216.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="FmdB family zinc ribbon protein"
                     /protein_id="HEX9916171.1"
                     /translation="MPTYEYECTACGHQFEESQKMTDKPIEICPKCKGKVKRKISGGA
                     GVLFKGSGFYITDHRSESYKKKAREEKERSEPKLDAEKVKKGPDRKTEIKKDKRR"
     gene            2847..3653
                     /locus_tag="VGB16_00390"
     CDS             2847..3653
                     /locus_tag="VGB16_00390"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_013037124.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0046873 - metal ion transmembrane
                     transporter activity [Evidence IEA];
                     GO_process: GO:0030001 - metal ion transport [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="ZIP family metal transporter"
                     /protein_id="HEX9916172.1"
                     /translation="MLESIQSLNPVILALVAGIFTWGMTALGSLSVFFTKKPSRKLLD
                     SMFGFAAGVMLAASFWSLLAPSIEMSTGKAIAKWFPAAVGFMLGSGFILAIDRLLPHL
                     HFGFPVEEKEGIKTNLHLTTLFALAVTLHNIPEGMAVGAAFGSVTKDISSLMTATTLA
                     IGIGVQNVAEGAAVAMPIKCEGVSCVRSFNYGQLSAVVEPIAALLTALMVTSFQPIMP
                     YALAFAAGAMVYVVVEEVIPESQKAGNADLATISTLVGFVLMMVLDVAFT"
     gene            complement(3803..4837)
                     /gene="rlmN"
                     /locus_tag="VGB16_00395"
     CDS             complement(3803..4837)
                     /gene="rlmN"
                     /locus_tag="VGB16_00395"
                     /EC_number="2.1.1.192"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_013387251.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008757 - S-adenosylmethionine-dependent
                     methyltransferase activity [Evidence IEA];
                     GO_function: GO:0016433 - rRNA (adenine) methyltransferase
                     activity [Evidence IEA];
                     GO_process: GO:0031167 - rRNA methylation [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="23S rRNA (adenine(2503)-C(2))-methyltransferase
                     RlmN"
                     /protein_id="HEX9916173.1"
                     /translation="MVNKINLKDLWTEELEDFLQKLEGKKYQAKQIAKWVFQKGVSDF
                     DLMTDLSKKMRDLLKDKTYVSNLKVLKAQKSKIDQTEKYLFELEDKETIETVLIKEKQ
                     RNTLCISTQVGCTLDCAFCATGKMGLKRNLFSGEIIDQIIQVKKLIPPQEKLDNIVMM
                     GMGEPLLNYDNAVKAIRIIASELGPSISAKRITLSTCGIVPQIEKLAKENLKIKLAIS
                     LNATTDQIRNNLMPINRKYPLDKLLPAVKSFAQKTKRRITFEYVLIKDVNDSDEDAFR
                     LAKLIQGISCKINLIAYNPIAGVKFEKPDNERIKKFRDILFPRCPAVTMRKSKGEDIL
                     AACGQLRGSQ"
     gene            complement(4859..>5504)
                     /locus_tag="VGB16_00400"
     CDS             complement(4859..>5504)
                     /locus_tag="VGB16_00400"
                     /inference="COORDINATES: protein motif:HMM:NF012692.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=2
                     /transl_table=11
                     /product="ROK family protein"
                     /protein_id="HEX9916174.1"
                     /translation="PIFMDNDANLMALAESRMGAARGYKYALCLTIGTGIGGGIILDN
                     NLFRGANFSGAEFGHMIICHHGIKCNCQSSGCLEMYASASAMIRITKMLLKQGRKSLI
                     PKLVSGKLNRITTETIFRAEKLKDKVAREVVSDTARFLGIGITSAINLLNPQIVVVGG
                     GVAQAGRKFIHLIEKETKKIGFPAITKNLIITGAKLGNDAGFIGAAILAAEQPN"
BASE COUNT         1615 a         1065 c         1258 g         1566 t
ORIGIN      
        1 ggaacaaaag ataaggtttt catcttcggc gattgtgcag ttttaccgaa tccggatgtt
       61 gaacagcttg ccagcatagc catctcttca gccagaacta tgaaggagct tattggcgaa
      121 gagccgaaag tggcaatgct ttccttctcc accaaaggaa gcgcccagca cgagatggtg
      181 gataaagttg ttgaagcaac cagactggtc aaacagcaag ttccggattt acaaatagat
      241 ggtgaactgc agttggatgc tgctataatt ccagatgtgg caaaaaagaa atcacccaac
      301 ggcccggtag ctggcaaagc caacgttctg atattcccgg atttaaacgc cggcaatatt
      361 ggatacaaat tgacccaaag gctagcccag gcggaggcaa ttgggcctat aattcaaggt
      421 ttggctaaac cggctaatga cctatctcgc ggttgcagtg tagatgatat cgtcaatgta
      481 gttgctattg ctatgacttt agcttaagtc aacgggaact ccctacctgt aagggtgggg
      541 atgggggagt ttcctgttct tgtgtcattc ctgtcccgca tttctgcggg atagactgca
      601 gcagagcctg tcccgtactg gatacgggaa atccagaaaa ggaaagcaag tatggatccc
      661 cgattaaacc ctcggggatg acaaaacata gtaggggcaa ttcatgaatt gcccctgtaa
      721 acaacaagaa tcacgcctgt ccttcgacat gctcaggatg gtgagcaagg ccgaaccata
      781 aaggcgtgag aatctataat gaacaatgat ttcaaaaggt tctttttagc tggaattatc
      841 caagggtcca agaagggaag aaacttacat aatcaggatt atcgcaagaa aattagattc
      901 ctgctgacaa aatactttcc tcaagccgaa atcattgacc cggtcaaagt gcatccgaat
      961 tcagcggagt atgcttcgac caaaggcaaa agggtattcc acaggtcaat agagcaagct
     1021 aaggggtgcg attgtttcat agcatatttg ccaactgcca gcatgggaac tgcgattgag
     1081 atgtgggaat gcaagaacaa aagaattccg atatggacta tttcgccctt gaaggagaat
     1141 tggtcagtga agtttttatc ctccacagta ttcaaaaaca tgaaagagtt tgagaaattc
     1201 atatcaacta ggccctagcg aaaattgtct ttggctgtaa tagtatagtc tcagaagcga
     1261 ccaaatagac cccgttagat gcgaagctat ctaacggggt ggacagagaa aagaaagcaa
     1321 gatgagtccc gttagaatct ttttctaacg ggtaaaaaag aagggaagtt tctaacggga
     1381 tgagtagatt tggatgtata aaggataagt ttgatgatag agattatctg atgcgagcat
     1441 atcttccagt agtaaagcgt cctaaaaaag ttgactatac ccaaaagatg tcgccaataa
     1501 gagaccaagg agaagaaggg acctgcgtgg gatttgcagt tgcttcgggg atgaaggagt
     1561 atcaggagtc tttagattat gagaagttgg tagaactctc cccgcgtttt gtttatagtg
     1621 aagctaaaaa aattgatggt gtgcctggat tagaagggac tactatccgg gcaagtatgc
     1681 aagtgcttga gaagatagga gtctgtcagg agaaactctg gccatattct ccccatcaaa
     1741 aggataaacc gaaagttgga gctaaagcca atgcccagaa ctttcgcatc attacttatg
     1801 cgcgcatact taatctaaat gagctgcgga tggcacttct tagtaaggga ccctgtgtct
     1861 taggaatcga ggtctttaaa ggaatgctta agactaagac cggcatcgtt cccttgccta
     1921 ggaagaatga aacaggctta gggggtcacg caattactgc ttgtggttac gacgacgaaa
     1981 aaggactggt taaagttaaa aattcatggt ctgaaaaatg gggcgataaa ggctatggat
     2041 acttacccta tgcctatatt gagcgttaca tgatggatgc ctggagttca gttgatattg
     2101 aggatcccaa tccacttact ctggcgagtg tgttgaatta taaaaagagg gcttcggtgt
     2161 gaaattcaaa accgttcgta aaaaaataaa agatgaaaca agatgatgca cttataaaac
     2221 ttgaggaaaa agagaacgca attttattta agtttggttg gatgtttagg aattctctaa
     2281 atgaatttag tacatcggtt gatagaagtt taggatatgc tgctaaaatt gttttaccaa
     2341 tgatcatggc tagtaaagaa gataagtcat ataatccatt tgctcaaatc attgaaaaat
     2401 acgctataca tatcttaata cataaattgg aaaaagaagg atacaagctc cttccttaaa
     2461 gcacaacaaa agaattccgt aaaattagaa ttagagttta gttttactca aaatgaggtg
     2521 tgaagatgcc gacttatgaa tatgaatgca ctgcctgcgg acatcaattt gaggaatccc
     2581 agaagatgac agacaagccc atagagatat gtcctaagtg caagggaaaa gtgaagagga
     2641 aaatatccgg tggggcaggg gttcttttta agggaagcgg attttacatt acagaccaca
     2701 gaagcgaatc gtataagaaa aaagcaagag aggaaaagga gagaagcgag ccgaagttag
     2761 atgcagagaa agttaagaag ggcccagaca ggaaaaccga aatcaagaaa gacaagagaa
     2821 ggtagtctgt aagtctaaaa caaaagatgc tagaatctat tcagagcctt aatcctgtta
     2881 tcttggcgtt ggtggcggga atcttcacct gggggatgac tgcacttggt tccctttcgg
     2941 tttttttcac taaaaaaccc agcagaaaat tacttgatag tatgttcgga tttgccgccg
     3001 gggtaatgct ggcggcaagt ttttggtcat tgcttgctcc gtcaatagaa atgtccactg
     3061 gcaaagctat tgcgaaatgg tttccagccg cagtcgggtt tatgttgggc agtggtttta
     3121 tattagctat cgatagattg cttccccatc ttcatttcgg ttttccggtg gaagagaaag
     3181 aaggaataaa aaccaatttg cacctcacta ccctatttgc tttagcggta actttgcaca
     3241 acattcctga aggtatggct gttggtgctg ctttcggttc agttactaaa gatatatctt
     3301 ctttgatgac tgccactaca ctggcaattg ggattggagt tcagaatgtg gctgagggcg
     3361 ccgcggtggc aatgcctatt aaatgtgaag gggtatcgtg tgtgaggagc tttaattatg
     3421 gacagttgtc cgcagtggtt gagcctattg ccgctttgct taccgcactt atggttacca
     3481 gttttcagcc aattatgcct tatgcacttg cttttgcggc aggagctatg gtttatgtgg
     3541 tagttgaaga ggtgatacct gaatcgcaaa aagcaggaaa tgcagatctg gcaacgatca
     3601 gcacactggt tggttttgtc ctgatgatgg ttttggatgt ggcattcact tgaagttaaa
     3661 tagtagttat tttgagaatg gaaagctaaa gctttccatt acataaacaa tgtagagaca
     3721 gaacactgtt ctgtctctac tgcttattca atatgtgaag agcagggtaa gccagtggca
     3781 tgtgtcactg gcatttctgt aattactggc tacctctcaa ctgcccgcaa gcagccagta
     3841 tatcctcacc cttacttttt ctcatggtca ctgcaggaca acgagggaaa agaatgtccc
     3901 tgaatttttt aattctttcg ttgtctggtt tttcgaattt cacgcctgct atggggttgt
     3961 aagcaatcaa gtttatcttg caggaaattc cttgaataag tttagccaat ctgaaagcat
     4021 cctcatctga atcgttcaca tccttaatca agacgtattc aaaagtgatt ctccttttgg
     4081 ttttttgagc aaaagattta acagcaggca gaagtttatc cagaggatat ttcctattta
     4141 tgggcataag attgttcctg atttggtcgg ttgtagcatt caaggatatg gctaatttga
     4201 ttttcaaatt ctcctttgcc agtttttcta tctgtggaac aattccacag gtggatagtg
     4261 ttatcctctt ggcactaatc gaagggccaa gttctgaagc aataattctt atggctttga
     4321 ccgcattgtc gtaattaagt agcggctctc ccatccccat catcacgata ttgtcaagtt
     4381 tctcttgagg tggaattaac ttcttgactt gaattatctg gtcaattatc tcaccagaaa
     4441 acaaattcct tttaagtccc attttgccag tagcacaaaa agcacaatcc aatgtacatc
     4501 caacttgagt ggagatacag agtgtgtttc tttgtttttc ctttatcaaa accgtttcga
     4561 ttgtttcttt gtcttctaac tcaaacagat atttctcagt ctggtctatt ttcgacttct
     4621 gtgcctttag gactttcaag tttgaaacat aagttttatc tttcagcaga tccctcatct
     4681 ttttggacag gtcggtcatc aaatcaaagt cagatacgcc tttttgaaaa acccatttgg
     4741 caatctgctt cgcctgatat ttttttcctt caagcttttg caagaaatct tctaattcct
     4801 ctgtccagag atctttgaga tttattttat taaccataat gaagagctaa gtgggaaatc
     4861 agtttggctg ctcagctgct aaaatcgctg ctccgatgaa accggcatcg ttgcccagct
     4921 tggccccagt tatgataaga tttttggtta tggctggaaa tcctatcttt ttggtctctt
     4981 tttcaataag atgaataaac ttcctgccgg cttgagcgac cccgccgccg acaacaacta
     5041 tctggggatt caacagattt atggcactgg ttatgcctat gcccagaaat ctggcagtat
     5101 cggaaacaac ttctcgagca actttatctt tcaatttctc tgcccgaaag atcgtctctg
     5161 tggttatgcg attgagtttt cctgagacca acttgggaat tagcgatttc cttccctgtt
     5221 tcagaagcat cttggttatt cttatcatag cgctggctga agcatacatt tccaggcaac
     5281 cggagctttg gcaattgcac ttgattccgt gatggcagat aatcatatgg ccaaattcag
     5341 ccccggagaa attcgctcct cgaaagagat tattatccag aattatccct ccgccgatgc
     5401 cggttccgat agtcaagcac agggcgtatt tataacccct agctgcgccc attctcgatt
     5461 cagccaatgc cataagattg gcatcgttgt ccataaaaat agga
//
LOCUS       DASWLN010000016        25024 bp    DNA     linear   ENV 20-FEB-2024
DEFINITION  MAG TPA_asm: Candidatus Zixiibacteriota bacterium isolate
            SMAG_U2949 k141_905289, whole genome shotgun sequence.
ACCESSION   DASWLN010000016 DASWLN010000000
VERSION     DASWLN010000016.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37960594
            Sequence Read Archive: SRR10376091
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Candidatus Zixiibacteriota bacterium (soil metagenome)
  ORGANISM  Candidatus Zixiibacteriota bacterium
            Bacteria.
REFERENCE   1  (bases 1 to 25024)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 25024)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (26-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/12/2024 08:28:26
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 1,440
            CDSs (total)                      :: 1,416
            Genes (coding)                    :: 1,412
            CDSs (with protein)               :: 1,412
            Genes (RNA)                       :: 24
            rRNAs                             :: 1 (16S)
            partial rRNAs                     :: 1 (16S)
            tRNAs                             :: 21
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 4
            CDSs (without protein)            :: 4
            Pseudo Genes (ambiguous residues) :: 0 of 4
            Pseudo Genes (frameshifted)       :: 0 of 4
            Pseudo Genes (incomplete)         :: 3 of 4
            Pseudo Genes (internal stop)      :: 1 of 4
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..25024
                     /organism="Candidatus Zixiibacteriota bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_905289"
                     /isolate="SMAG_U2949"
                     /isolation_source="soil"
                     /db_xref="taxon:2053527"
                     /environmental_sample
                     /country="USA: Oregon"
                     /lat_lon="42.99 N 123.63 W"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            <1..263
                     /locus_tag="VGB16_00405"
     CDS             <1..263
                     /locus_tag="VGB16_00405"
                     /inference="COORDINATES: protein motif:HMM:TIGR04183.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0031240 - external side of cell outer
                     membrane [Evidence IEA]"
                     /codon_start=3
                     /transl_table=11
                     /product="T9SS type A sorting domain-containing protein"
                     /protein_id="HEX9916175.1"
                     /translation="QNYPNPFNPSTIIEYYLSKPCHVTLNIYNVLGQKVRTLKNEYQG
                     IGKGAVSWEGKDDRGKDMPSGTYFYKLKAGDLTESRKMLLIK"
     gene            complement(490..975)
                     /locus_tag="VGB16_00410"
     CDS             complement(490..975)
                     /locus_tag="VGB16_00410"
                     /EC_number="3.6.-.-"
                     /inference="COORDINATES: protein motif:HMM:NF012514.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0046872 - metal ion binding [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="NUDIX hydrolase"
                     /protein_id="HEX9916176.1"
                     /translation="MKQKEFANLLESMKTGKNCDVTLFIFKDNKIVAIAKPWYQEGLY
                     RAPSGRPKLNENLMDAIHREALEETGAKIRLKRYILRISVIFQHNRQKVNWTSHVFLA
                     DYFSGNLEPIDKREIKKVNLLTLDELASLKGKLLKQKSGGLAYRATLTEETIKIIKTM
                     G"
     gene            complement(1050..1412)
                     /locus_tag="VGB16_00415"
     CDS             complement(1050..1412)
                     /locus_tag="VGB16_00415"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_018206155.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="cupin domain-containing protein"
                     /protein_id="HEX9916177.1"
                     /translation="MKITNFKDGSFKKVDDPKAEDVEMQVLISTEDGAPNFAMRVFKV
                     KPNGYTPYHTHNWEHEVFILKGEGTITVEKKETKFKYGDAIFVPPNVMHQFKNSSKEE
                     LFFLCLIPAQNICALPQK"
     gene            complement(1641..3023)
                     /locus_tag="VGB16_00420"
     CDS             complement(1641..3023)
                     /locus_tag="VGB16_00420"
                     /inference="COORDINATES: protein motif:HMM:NF014910.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003824 - catalytic activity [Evidence
                     IEA];
                     GO_function: GO:0050660 - flavin adenine dinucleotide
                     binding [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="FAD-linked oxidase C-terminal domain-containing
                     protein"
                     /protein_id="HEX9916178.1"
                     /translation="MQKNIKNIVADLAKLIGKEHILSSYEERICYAYDATNCFFLPDI
                     VVLPIKTEQVSRVMKYAFENDVPVVPRGAGSGYTGGSLPVKGGIVISLERMNKILEIN
                     KGKSYAIVEPGVVTLDFAQEVAKLGLLYPPDPSSDKMSTLGGNVGENAGGLKGRKYGV
                     TKNYVLGLETVLYNGEVLEAGIFNLDEKGYDLTSLLIGSEGTLGVITKIAFKLLPQVP
                     YEETILASFEQMEDAAQVVADITSAGIIPSVLEFIDKDTLDCVLDYIQVNFVQKGGPI
                     LLIEVDGQNKGEVHPELEKIEQICQKNNAKKINFAQNKEEKEKLWKIRKSASASLLRK
                     APTKVNEDICVPPSQLPDMVRGIKELAKKYNISINTFGHAGDGNLHVNIMTDRRKKEE
                     MERVEKAVEELFDLTLKLDGTLSGEHGIGTTKLKFLEREVGKSGIKLMRNIKKAFDPK
                     GLINPGKIFN"
     gene            complement(3041..4006)
                     /locus_tag="VGB16_00425"
     CDS             complement(3041..4006)
                     /locus_tag="VGB16_00425"
                     /EC_number="3.1.21.-"
                     /inference="COORDINATES: protein motif:HMM:NF013579.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003677 - DNA binding [Evidence IEA];
                     GO_process: GO:0006304 - DNA modification [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="restriction endonuclease subunit S"
                     /protein_id="HEX9916179.1"
                     /translation="MERLDKMFYIQYGDKNFTNKSYLDEGTTLLIASQGVDNGGYGFF
                     DIKERFHAPIITVPRTGSIGFAFVQLNPCAVTDDCMVLTPKRKMSIEYLFYISAIIRR
                     SKWRFNYGRKITPRRLSEMHALSPEEFKTPLSYESLYKKLYPRKNFNDSSNKIGKPQT
                     KEFQITELFNLERGHFHAIDRLEDGIYPTISRVSVDNGLVGFYRKPRRAKVFSKGLLT
                     ISSVTGDAFLQHTPFIATDNVVICIPKKSLQITTLIYIQALLNRVKWRYSYGRQCYKG
                     NFQKTTLSLPVSGSSSINEGYIEQIVMAQPYWREFKERILDNVKN"
     gene            complement(3990..5876)
                     /locus_tag="VGB16_00430"
     CDS             complement(3990..5876)
                     /locus_tag="VGB16_00430"
                     /inference="COORDINATES: protein motif:HMM:NF014442.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003677 - DNA binding [Evidence IEA];
                     GO_function: GO:0008170 - N-methyltransferase activity
                     [Evidence IEA];
                     GO_process: GO:0006306 - DNA methylation [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="N-6 DNA methylase"
                     /protein_id="HEX9916180.1"
                     /translation="MTKRRSSSQSEVHAYFYILDELVNKKGWEKNQIYTQQECLQVKS
                     IAECLGKSKPENMVKINETTCYVIEAKNERAKINIALKEAKDDYANLINKSKLIKAPF
                     VTGIAGNGDEGFVAKSQFLKNGKWETITENDVEVTGLLSKNQIEKVLSTQDPHIKEVE
                     INEKEFLRTAEEINGILHEGGIHKDNRARVMSAILLALVEGTEINIDEEPVILIQSIN
                     SRVELVLKKHKKPNYSRFISIDLPASEDNHIKFKKAIVLTIQELLGLNIRSAMQSGKD
                     VLGTFYEVFLKYGNGAKEIGIVLTPRHITKFAAQVLDIQANDLVYDPTCGTGGFLVAA
                     FDEVKKKATTEEFDKFKKYGLYGVEEQDPVVSLALVNMIFRGDGKNNIVDGNCFAKWL
                     NVKSRNGIIQAEYLSKDTPHRVPPVSKVLMNPPFPKKKTDYKEYLFIEQALKQMQNEG
                     ILFSVLPYSCMIKGGGYREWRKRLLINNTLLSVVTFPEDLFYPVGVHTLGIFVKKGIP
                     HLKNQKVLWIRALNDGRWKKKGKRLEHPKAKDDYSIILPMLREFIQKQPKKVDSVAKF
                     QKACEIDFSDSDLQLVPEAYLDEQKISYEDITKDIEQLIRENIAFKIKFEHQLEKVEN
                     GTTG"
     gene            complement(5993..7078)
                     /locus_tag="VGB16_00435"
     CDS             complement(5993..7078)
                     /locus_tag="VGB16_00435"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011417384.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="Ldh family oxidoreductase"
                     /protein_id="HEX9916181.1"
                     /translation="MPVVNDVKLTNFCVDIFKKIGVSEADAKIVADNLVTSDLRGISS
                     HGVARLKRYVDGIKGGIILPNVKPKILKETPVTVAVSGENGLGQPAGFFAMNLAIEKA
                     KKNGLGFATVCNSNHYGIAGYYALMALKHDLIGISLTNSEPLVVPTFSKDMIIGTNPI
                     SVAIPAGKERPYVLDMATSVVPRGKFEVYLRQEKPVPLGWAVDQEGLPTTDTKLVIDN
                     MTNTAGGGILPLGGEGELLSGHKGYGLSLLVDILSGVLSGAEFGPYVKSKKRGTKTFV
                     NVGHFFGAVKIENFIELDKFKSTMDSMIQQLKNSQKAKGQSRVYIHGEKEFEKWEEHK
                     KNGLPLNDKVVETLKQLGQELGVPYNL"
     gene            complement(7105..8370)
                     /gene="larA"
                     /locus_tag="VGB16_00440"
     CDS             complement(7105..8370)
                     /gene="larA"
                     /locus_tag="VGB16_00440"
                     /inference="COORDINATES: protein motif:HMM:NF033504.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0050043 - lactate racemase activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="nickel-dependent lactate racemase"
                     /protein_id="HEX9916182.1"
                     /translation="MKIELKYGDSHLDLNLDEKNLLGILKLKEAPGLPDVNLGLRQAL
                     DNPIGTKKLSQIAKGKKTVCIVVADYTRGVPYKRESYNMLLPIVDELRAEGVKDKDIK
                     FLVGTGAHRPHTQKENIDNFGEEIVKNFEVISHDCRANNISLGKLSTGNQLWVDRLWV
                     ESDVKILTGMITTHYFGGFSGGRKGILPGIAGYETIRANHAMIIHPQVDIAKTKGNPI
                     SDEMDEAAGKAKVNFIVNVVINDKKQIVKILAGDLRKAFDSGAEACRNIYKVEVNKLA
                     DVVFACSGGYPKDVSLYQSQKAINNAKLVLKEGGTIVLVAECREGIGSDTFGQWLYEA
                     KDLDGLLRTDPKKITVGGHTAVGNAKVLKKFKILVVSKIEKEILEKRFYHHAQTLDDA
                     VKSVREWYGEDYKAYVMPQAGLIYPYYRI"
     gene            complement(8411..9673)
                     /locus_tag="VGB16_00445"
     CDS             complement(8411..9673)
                     /locus_tag="VGB16_00445"
                     /inference="COORDINATES: protein motif:HMM:NF014774.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0046872 - metal ion binding [Evidence
                     IEA];
                     GO_function: GO:0051536 - iron-sulfur cluster binding
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="(Fe-S)-binding protein"
                     /protein_id="HEX9916183.1"
                     /translation="MPQTQPDFKYLSKHQDQLLKCLKCGTCLSVCPIYAELKYEPASA
                     RGRMALIQQVFEGNLDLTDLFRKKINFCLNCKSCVEACPGGVRVDDIILAARAELVNR
                     GKLTFVERLIFRHLLKRGRLLPPVGRFAVFMGSLAQKVLPQKSPINLFLPLPQGWKQR
                     IYPKISRKPLRKRIPEVVKAKNPKMRVGYFYGCGTNLVYPQVGEAMIKVLTHNDIEVV
                     IPKKQICCGTSIYNSGDFKTAIKHAKRNLKVFKDAKVDCIVVNCASCGLTLKKEYKEL
                     LGVDFDIPVYDINEFLIDKIEMKKDFKKELNYTVVYHDPCHLNRGQGIKSQPRKILKM
                     LPGLKFIDMKNADRCCGGGGSFHLKYYPTSEAIMEYKLKEIQKIEPDFLVTSCPGCMM
                     RFEETFLQGKMKTKIKNIIELVAEAYGD"
     gene            complement(9893..10648)
                     /locus_tag="VGB16_00450"
     CDS             complement(9893..10648)
                     /locus_tag="VGB16_00450"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEX9916184.1"
                     /translation="MYRKISYLGSVIVLASIVICMILFTDCSYTATKKVKEKITPRAY
                     IKEDQSTAVYIDGNSKPILRIEDLPKEFPPHIHHPEGARFPDTRFNLASLSPDGKKIV
                     FSCGLVHNWVGLFGLKSKKIDVISWLFDSDIDQILWSPNSRHFAYTFVAPSGEFVVET
                     VSLRQKTQEPYISNWWGAIKDGGFRWDENINIYDFQWSEDGESIQFTAQKSEIKDNKE
                     VRMESEPEKIITLRAIKEETVKETPKKEKVKKP"
     gene            complement(10678..10750)
                     /locus_tag="VGB16_00455"
     tRNA            complement(10678..10750)
                     /locus_tag="VGB16_00455"
                     /product="tRNA-Lys"
                     /inference="COORDINATES: profile:tRNAscan-SE:2.0.12"
                     /note="Derived by automated computational analysis using
                     gene prediction method: tRNAscan-SE."
                     /anticodon=(pos:complement(10715..10717),aa:Lys,seq:ctt)
     gene            complement(10842..10913)
                     /locus_tag="VGB16_00460"
     tRNA            complement(10842..10913)
                     /locus_tag="VGB16_00460"
                     /product="tRNA-Lys"
                     /inference="COORDINATES: profile:tRNAscan-SE:2.0.12"
                     /note="Derived by automated computational analysis using
                     gene prediction method: tRNAscan-SE."
                     /anticodon=(pos:complement(10879..10881),aa:Lys,seq:ttt)
     gene            complement(10969..12243)
                     /locus_tag="VGB16_00465"
     CDS             complement(10969..12243)
                     /locus_tag="VGB16_00465"
                     /EC_number="6.3.4.4"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011345530.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004019 - adenylosuccinate synthase
                     activity [Evidence IEA];
                     GO_function: GO:0005525 - GTP binding [Evidence IEA];
                     GO_process: GO:0006164 - purine nucleotide biosynthetic
                     process [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="adenylosuccinate synthase"
                     /protein_id="HEX9916185.1"
                     /translation="MPNWVIVGTQWGDEGKGKVVDLLTEKADIIARPQGGANAGHTVV
                     IGDNLFILHLIPTGILHPHKICIIGNGMVIDLEQLFFEIADLKKRGIDVKNRLFISEN
                     AHLVMPYHKTIERLEEESKGRNKVGTTGKGIGPAYRDKIGRCGIRLRDILYPEVFKKK
                     VESNLKNNSHLFRKLKRGEFSSLKTESGVILKYKKQVLSLMADVSLILNEAIRNGKSV
                     LFEGAQGAMLDIDHGTYPYCTSSNVSAGGACTGTGVGPTLIDEVIGVVKTYTTRVGNG
                     PFPTEFDDGLCEAIREKGKEFGATTGRPRRCGWLDLVLLKYSVRVNGITKIALTKLDV
                     LDDLGKIKVCITYKYKSKTIENFTNDLKFLENCTPVYKEIDGWKTSTHGMTKYENLPQ
                     RAKDYINFISQQLGVKVMLISTGCKRNETILI"
     gene            complement(12320..12907)
                     /gene="clpP"
                     /locus_tag="VGB16_00470"
     CDS             complement(12320..12907)
                     /gene="clpP"
                     /locus_tag="VGB16_00470"
                     /EC_number="3.4.21.92"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_013276523.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0009368 - endopeptidase Clp complex
                     [Evidence IEA];
                     GO_function: GO:0004176 - ATP-dependent peptidase activity
                     [Evidence IEA];
                     GO_function: GO:0004252 - serine-type endopeptidase
                     activity [Evidence IEA];
                     GO_process: GO:0006508 - proteolysis [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="ATP-dependent Clp endopeptidase proteolytic
                     subunit ClpP"
                     /protein_id="HEX9916186.1"
                     /translation="MTLIPYVIEQTTRGERAYDIYSRLLKDRIIFIGQPIDDNLANSV
                     IAQMLFLEADEPEKDINIYIHCPGGIVTAGLAIYDTIQFVKPDVATICVGIAASMGAV
                     LLTAGTKGKRSALPNSRIMIHQPWGGVQGQVTDIEIQTKEMIKTREMLNRILTKHTGQ
                     PLEKIEKDTDRNFFMSAEEAKAYGLIDEIFEKRKK"
     gene            complement(12904..14190)
                     /gene="tig"
                     /locus_tag="VGB16_00475"
     CDS             complement(12904..14190)
                     /gene="tig"
                     /locus_tag="VGB16_00475"
                     /EC_number="5.2.1.8"
                     /inference="COORDINATES: protein motif:HMM:TIGR00115.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0005854 - nascent polypeptide-associated
                     complex [Evidence IEA];
                     GO_function: GO:0003755 - peptidyl-prolyl cis-trans
                     isomerase activity [Evidence IEA];
                     GO_function: GO:0051082 - unfolded protein binding
                     [Evidence IEA];
                     GO_process: GO:0051083 - 'de novo' cotranslational protein
                     folding [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="trigger factor"
                     /protein_id="HEX9916187.1"
                     /translation="MKVNLTEDRGWKRTLEIELPAEKVDEQFDLFQEKYRKKAKIKGF
                     RPGKAPLEIVKEKYKDAIARDVLDSLIPKIFQDAIKQTNLSPITVPIVKELDFDYGKP
                     LKVKAELEVIPEFEPKDYQGVKLVRKVSEITEKEVERALDYLREKNAQLRAVERESKS
                     EDFLVLDLERTMLDDQKKKPEKFTNQLVQLGSQNIFKEFQEGLEKTKAKDQKKIELTY
                     AQDYFEKKLAGKKAHFNIEVKEVKEKVLPELNDDFAKSLGDYQDLNQLKNRIKEDLER
                     KAKEDSETRLKSDLIQKIVEVNSFEVPGSMVQRYLDAAVNDFKKQYDKVDEEKIREQY
                     RSIGTDRIRWNLIYHRLAEKEKMDTEPQEIEEWTKAFAKNYNLEINKAKEFLAKNREI
                     ENIKDTILEEKVLNYLLGKAEITEEKIKPAQGEKTK"
     gene            complement(14205..14289)
                     /locus_tag="VGB16_00480"
     tRNA            complement(14205..14289)
                     /locus_tag="VGB16_00480"
                     /product="tRNA-Leu"
                     /inference="COORDINATES: profile:tRNAscan-SE:2.0.12"
                     /note="Derived by automated computational analysis using
                     gene prediction method: tRNAscan-SE."
                     /anticodon=(pos:complement(14253..14255),aa:Leu,seq:tag)
     gene            14448..15083
                     /locus_tag="VGB16_00485"
     CDS             14448..15083
                     /locus_tag="VGB16_00485"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_006927598.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="redox-sensing transcriptional repressor Rex"
                     /protein_id="HEX9916188.1"
                     /translation="MKRISLSTIYRLSIYHRSLQRREERGIQTISSKELAREEKLTPA
                     QVRKDLSFFGSFGTRGLGYSVKQLKDNIAGILGINRQWNVALVGVGNVGSSLVSYKEF
                     PKQGFNIKLIFDNDQRKINKYHKEILVSDARELKTKLKESSIDIVIIAVPASVAQEVV
                     NQVVEAGVKAILNFAPTNVVVPEGVIIRSVNMAIELEHLSFALVNKSLFKA"
     gene            15413..16300
                     /locus_tag="VGB16_00490"
     CDS             15413..16300
                     /locus_tag="VGB16_00490"
                     /inference="COORDINATES: protein motif:HMM:TIGR04183.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0031240 - external side of cell outer
                     membrane [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="T9SS type A sorting domain-containing protein"
                     /protein_id="HEX9916189.1"
                     /translation="MKRKLLVLVLGISLFVLLGLSFALAGDYGEPDTVWLEKIQTVNP
                     NSQVVYRIYLFNDHNISAFTIPITFPDTTSNMDISLDSVSSVGTRIALAQGKNDPSFF
                     PTDSTNVVNKNRIALWMVWSWVGAGYLSPVTRTQALATPVVKIYFRTGPSWVSPNYVS
                     VDTTTMVSGSLLETDSVGVEFVPKLAGKGALDVTDITTPNLPMVFSLSQNYPNPFNPS
                     TTIRFTLPKDDEVTLMIYNVLGQKVKALIDHQDLPAGTKQVEWNGTDANNNPVASGTY
                     FYQITTENNRITKKMVLTK"
     gene            16678..18822
                     /locus_tag="VGB16_00495"
     CDS             16678..18822
                     /locus_tag="VGB16_00495"
                     /EC_number="3.2.1.23"
                     /inference="COORDINATES: protein motif:HMM:NF014502.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="beta-galactosidase"
                     /protein_id="HEX9916190.1"
                     /translation="MLKIENGKLLIGREEYYPISAELHYFRVNKKYWSVCFERIKKAG
                     FRIISTCIPWNLHEISLGDFDFFGETDPRRDLIVFLELAREFGFKVILKPGPYIGAEW
                     DNGGYPDFLFNNPEFLAKDSQGQSVEQASQVKVKSGLVPCYNHPRFQNQVKRYFSALS
                     DVIKGYTYPKGPIIMILLDNHPSFGRNMEPFKLDYNEYVVGTLYPQFLQEKYNQIKHL
                     NNVYVEKSKRFTDVQPPKHLKINQPKQLLKYLDWIEFKEKYLANYIENLKEIFVSCEI
                     PAIFSTVLFQEKLFSVPFNWTLIDREEKYVGAQVGMEKNYPDLINHLRYYATCSRFAW
                     ANEFRVGNCSDNPSDSRKYFPVSSRETKLQMVSSLSSGIKGYNYYMFVERDHWYDSPL
                     AEDGTLQTSYELIRKFNQAVEKIELEKFYSLAEIGLVNYRPYLWHTHLGIEKPYGYIL
                     NLVNLTSKNLASDLLTLNLDFGIADMWLADSLSKFKLLFMPVAEYMDADGQKNILELA
                     KAGKSVILFGLLPKYDERMKGCEILSKGLRLRTNQKEQIGEIKTSSEEFITQAYGFIR
                     RVGGKSQVIAELDNKPVGVKLKVGKGMVYFFSFDICARLDHSKLSFLRSVIDDCSISS
                     PIVTNHPFTYVVPHKANKKVVLYVVRWDKGEEEKVIIQVDCRKVGIKGARIRLIDLLG
                     EEVIKTTSSELKRGLAFKLGPLDSKMYLVETR"
     gene            complement(18823..19065)
                     /locus_tag="VGB16_00500"
     CDS             complement(18823..19065)
                     /locus_tag="VGB16_00500"
                     /inference="COORDINATES: protein motif:HMM:NF024578.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005515 - protein binding [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="PDZ domain-containing protein"
                     /protein_id="HEX9916191.1"
                     /translation="MLEDRAGVLVSFVEVEGSAGKAELLAADVIRKVEDKDVTDLKGF
                     KEILDQFKSKKEIMLTVKRGKSSCFVLLSNTGEEKQ"
     gene            complement(19684..20415)
                     /locus_tag="VGB16_00505"
                     /pseudo
     CDS             complement(19684..20415)
                     /locus_tag="VGB16_00505"
                     /inference="COORDINATES: protein motif:HMM:NF024758.4"
                     /note="internal stop; Derived by automated computational
                     analysis using gene prediction method: Protein Homology."
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="trypsin-like peptidase domain-containing
                     protein"
     gene            20748..21602
                     /locus_tag="VGB16_00510"
     CDS             20748..21602
                     /locus_tag="VGB16_00510"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_014854861.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003677 - DNA binding [Evidence IEA];
                     GO_function: GO:0003700 - DNA-binding transcription factor
                     activity [Evidence IEA];
                     GO_function: GO:0016987 - sigma factor activity [Evidence
                     IEA];
                     GO_process: GO:0006352 - DNA-templated transcription
                     initiation [Evidence IEA];
                     GO_process: GO:0006355 - regulation of DNA-templated
                     transcription [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="sigma-70 family RNA polymerase sigma factor"
                     /protein_id="HEX9916192.1"
                     /translation="MAKQPKRYRDQDRSLDLYLREIGETPLINAGEEVELAKRIRKGD
                     QAALDKLTRANLRFVVSVAKQYQNQGLSLADLINEGNIGLIKAAKRFDETRGFKFISY
                     AVWWIRQAILQALAEQSRIVRLPLNRVGTLHKIGKISSSLEQEFGREPSPDEIAKKLE
                     LTEMEVADTLKISNSHLSLDAPFSVSEDNSLMDVLEDEFQPSPDEALLDESLKLEIER
                     ALDSLTPREAEVINLYFGLNHEKALTLEEIGARFGLTRERVRQIKEKAIRRLKHASRS
                     RSLRAYLS"
     gene            21748..22056
                     /locus_tag="VGB16_00515"
     CDS             21748..22056
                     /locus_tag="VGB16_00515"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_009711926.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003700 - DNA-binding transcription factor
                     activity [Evidence IEA];
                     GO_process: GO:0006355 - regulation of DNA-templated
                     transcription [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="autorepressor SdpR family transcription factor"
                     /protein_id="HEX9916193.1"
                     /translation="MDEVFKALADPTRRKILDLLKKNDLTAGQIAEHFEISKPSISHH
                     LSVLKGADLVRTERDGQFLVCSINLSVMEEVAEIFFDLFKKKGAKSKSFSKKRDFPCS
                     "
     gene            22074..22676
                     /locus_tag="VGB16_00520"
     CDS             22074..22676
                     /locus_tag="VGB16_00520"
                     /inference="COORDINATES: protein motif:HMM:NF025015.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="SdpI family protein"
                     /protein_id="HEX9916194.1"
                     /translation="MAIFFYQILPQRFAIHWGADGNPNGFANKSFWSVSFGPFTIIAI
                     YLLFLIIPKIDPRKENIAKFKQYHLFRNVLLVFFLFFEYLTLRTAASPNQKLSSSYII
                     FGLGLLFIFIGNYLPKMSSNFFVGIRTPWTLSSDEVWKETHRIGGKLFALMGILFLLG
                     SFLPKAVSWVLFIPLVGCIFFIVIYSYYLYKKSKIKQAQG"
     gene            22703..24361
                     /locus_tag="VGB16_00525"
     CDS             22703..24361
                     /locus_tag="VGB16_00525"
                     /inference="COORDINATES: protein motif:HMM:NF042769.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="alpha/beta hydrolase"
                     /protein_id="HEX9916195.1"
                     /translation="MKFTKWVMFAALLLSMFFLSEKSFSDQTFLVKLDGKKVGDEIFS
                     YRTDKKDTNVVIFSSKTKISMEGMGVSIEQEEKLNKRNYAFSEYQLKADMAGQYQEME
                     AKVEGDSIKASAFINMQEYEKKVPYRESDVVLGLPTLSVYFYQILLNRYDFNKKGTQQ
                     FHAYLPDKLSEGPLKVTLKEKYPAKLKEEGFQVDHLEAVLVNLVINIWAKSDNHQLLK
                     VDIPFMKFESSLPELSIIGKEEVKTPDIISANNFVEKEITFKSGEFEISGTLSLPKDI
                     KGKFPAVILVQGSGPQDRDETVGPNKPFKDIAHALSNSGFAVLRYDKRTYTYKRPKLD
                     LTNITLKEEVIDDAIWGIKFLREQAEVIPQKIFVLGHSLGAECTPLIALEDKNLAGVI
                     MLAPSARPADSLILEQIEFQAKVAGPSASQEATKQLEELKKGFAQIRNKTFPENQMLF
                     FTSGKYWYYWLSYSAADTIKKIDCPLLLLQGEKDCQVSMKDFEIWNGVLKGRGNSTLK
                     SFANLNHLFMYVEGKSTGEEYQRAGHIDGEVLKALIEWIKKYSM"
     gene            complement(24459..>25024)
                     /locus_tag="VGB16_00530"
     CDS             complement(24459..>25024)
                     /locus_tag="VGB16_00530"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=3
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEX9916196.1"
                     /translation="IFLLLAIIVFAIVPKNVEKIKNKIDHDFWISFLVGLAVLLLIAP
                     AFLILFVLLCITIIGIPVALIALPLAVAAYFLLAYTSGAYFLGNKIKEKTSIKPETPM
                     MTLIVGVLAVEVVLLFARLIGVIGGPLVVLGWIMAILGWVIFGVIITVGLGAGLLTRF
                     GTRPKDAKPEEKPKETQDNSCSQPNNK"
BASE COUNT         7015 a         5365 c         4851 g         7793 t
ORIGIN      
        1 cccaaaacta tcccaatccc ttcaatccaa gcactattat cgaatactat ctatccaaac
       61 cttgccacgt aaccctgaat atttacaacg tccttggcca gaaagtaaga acactgaaga
      121 atgaatatca aggtattggc aagggagcag tcagttggga ggggaaggat gataggggta
      181 aagacatgcc cagtggtact tacttctaca aattaaaagc aggagatttg actgaaagca
      241 ggaagatgct gttgatcaaa taagaataga tagcagaatt acgtagtgga tttcatgatt
      301 cgggtgctga aaatgggatt taaattctaa cgcaacggcg caaccgttgc ttacccttat
      361 cataacctat atatgggtag ggaacgactg ctgccgttcc ctaaggctgt aaaaaagtaa
      421 tggcgcagcc attacttacc cgttgaggtg acctgttctt ctatggaaaa gtaatggcgc
      481 agctgttact taccccattg tctttattat ctttatcgtt tcctcagtca aagtcgcccg
      541 ataagccaaa ccacctgatt tctgcttaag cagtttgcct ttcaaagagg ccaattcatc
      601 taaagtcaaa agatttactt tttttatttc ccttttgtca atcggctcta agtttccaga
      661 aaaataatca gctaagaata cgtgggaagt ccagttgact ttttgtctat tatgctggaa
      721 aatgacgctt attctcaaaa tatatctttt caatctgatt tttgcaccgg tctcctccaa
      781 agcttcacga tggatggcat ccattaagtt ttcatttaat tttggtcttc cgctgggtgc
      841 ccgatagagt ccctcttggt accagggctt ggcgatggca actattttat tgtctttgaa
      901 tataaataat gtcacatcgc aatttttgcc ggttttcata ctttccagaa gattggcaaa
      961 ctctttttgc ttcatcttaa aattcatctt caagctcttt tatttctttt cttgaaccca
     1021 agatttctcc gataaaaatc aactcggccc tatttttggg gtagagcgca gatattttga
     1081 gccggtatca agcagaggaa aaacaattct tccttggagc tgtttttgaa ttggtgcatc
     1141 acattcggag ggacaaaaat cgcatcccca tatttgaatt tggtttcttt cttctcgact
     1201 gtaattgttc cctcgccttt taatataaaa acctcatgct cccagttatg agtgtgatag
     1261 ggggtatatc cgttgggctt cactttgaag acgcgcatag cgaaattggg ggcaccatcc
     1321 tcagttgaga ttaaaacctg catctccaca tcttcggctt ttgggtcatc cactttttta
     1381 aaagagccgt ctttaaaatt tgttattttc atacctcctc cattttggga tagacttcaa
     1441 tagtaggggt tcaaaatttt gaacccctac ataatgcaag attgcttcgt cgtccttcga
     1501 catagctcag gactcctcgc aatgacagcg tttagaggcc attcataacg cctacaaatg
     1561 tcagtaagag tttagatttt aacacaaaca atagcgtcag gagtaattcc tgacgcaaca
     1621 gagaattcca ggcaataatt ctaattaaat atcttccccg gatttatgag ccctttaggg
     1681 tcaaatgcct tttttatatt tctcataagc ttaatccccg atttcccaac ttccctttcc
     1741 aaaaacttca acttggttgt tccgatgccg tgctccccgg aaagcgtccc atccaatttc
     1801 aaagtcaaat cgaaaagctc ctccaccgct ttttccaccc tttccatctc ctcttttttt
     1861 ctacggtcag tcattatatt cacatgtaga tttccatctc cagcatgacc aaaggtgttt
     1921 atgcttatat tatatttctt agccaactct tttattcccc ttaccatatc agggagctga
     1981 ctgggcggaa cgcagatatc ttcatttact ttagttggcg cctttctcaa aagtgaggcg
     2041 gaagctgact tgcggatctt ccacagtttt tctttttcct ccttattttg ggcaaaattt
     2101 attttcttag cattattctt ttggcaaatc tgttcaattt tctccagttc cgggtgaact
     2161 tcacctttgt tttgtccatc tacttcaatt aaaagaattg gtccgccctt ttgtacgaaa
     2221 ttgacctgga tgtaatccaa aacgcagtcc aaggtatcct tatcaatgaa ttccagaacc
     2281 gaggggatga tgccggctga ggttatatct gccaccactt gtgcggcatc ctccatctgc
     2341 tcaaaggaag ctaagatagt ttcctcgtaa gggacttgag gtaaaagttt gaaagctatc
     2401 ttagtgatca ctcccaaggt accctctgag ccgatcaaaa gggaagtgag gtcgtaccct
     2461 ttttcatcca gattgaagat gccggcctcc agtacctctc cattatataa gacagtctct
     2521 aatcctaaaa catagttctt agtcacacca tattttcttc ctttaaggcc tccagcgttc
     2581 tcacccacat ttcctcccag ggtggacatc ttatcgctcg atgggtcagg tggatagaga
     2641 agtccgagct ttgccacttc ctgggcaaaa tccaaagtta ccacccctgg ctcaacaatg
     2701 gcataacttt ttcccttgtt tatctccaga atcttattca tccgttccag gcttataact
     2761 attccaccct taactggcaa gcttccaccc gtgtaacctg agccagctcc tcgcgggaca
     2821 acgggaacat cattttcgaa agcatacttc atcaccctgg agacttgctc agtcttgatt
     2881 ggcaaaacta caatatccgg caggaagaaa caattggtgg catcataggc ataacatatc
     2941 ctctcctcat aggaagagag gatatgctct ttaccaatca attttgccaa atcggcaaca
     3001 atatttttta tgttcttttg cattgtttaa ttttttagat tcaatttttg acattatcta
     3061 aaattctttc cttaaactct ctccaataag gttgagccat tactatttgt tcaatatacc
     3121 cttcgttaat actactactt ccactgaccg gaagagaaag agttgttttt tgaaaattcc
     3181 ccttatagca ttgcctacca tatgagtaac gccatttcac tctatttaat agtgcttgaa
     3241 tatatataag cgtcgtaatt tgtagagact tctttggtat acatataact acattgtcgg
     3301 tggcaataaa aggcgtgtgt tgcaaaaaag catctcctgt cacgcttgat attgtgagaa
     3361 gccctttcga aaataccttc gccctccttg gcttcctgta aaaaccgacc aagccattat
     3421 caaccgacac tcttgagata gtcggataaa ttccatcttc taatcgatcg attgcgtgga
     3481 aatgccctcg ctctagatta aatagctccg taatttgaaa ctctttggtt tgaggtttcc
     3541 caatcttatt ggaggaatca ttgaaattct ttcgtgggta tagtttctta tacaaagatt
     3601 catatgataa gggagtttta aattcctccg gggaaagggc atgcatttca ctgagtcgtc
     3661 gtggagtgat ttttcgaccg tagttaaatc tccatttaga acgtctaata atagcagaaa
     3721 tataaaaaag atactcgatg ctcatttttc ttttgggtgt gagaaccata cagtcatctg
     3781 ttaccgcaca tggattgagt tgaacaaacg cgaaccctat actacctgtt cttggtacgg
     3841 ttattatcgg ggcatgaaat ctttctttta tatcgaaaaa cccatatcca ccattgtcaa
     3901 cgccctgaga tgctatgaga agagtagtcc cttcatccaa ataacttttg ttggtaaaat
     3961 tcttatcgcc atattggata tagaacattt tatccagtcg ttccattttc aaccttctcg
     4021 agttggtgtt caaattttat tttaaacgct atattttctc ggatcaattg ttcaatgtct
     4081 ttggtaatat cttcataact tattttttgc tcatctaaat atgcttctgg gacaagctgc
     4141 aaatcactat ctgaaaaatc aatttcacat gccttttgga atttagcgac tgaatcaacc
     4201 ttcttcggtt gtttctgaat aaattccctg agcatcggaa gaatgataga ataatcatct
     4261 tttgccttag gatgttctaa tcttttcccc tttttcttcc atcggccgtc atttaatgcg
     4321 cgaatccaca atactttttg gttctttaaa tggggaattc ccttcttcac aaaaataccc
     4381 aatgtgtgta ctcccactgg ataaaaaagg tcttcaggaa atgttacaac cgataatagt
     4441 gtattattta tcaataacct tttcctccat tctctatagc ctcctccttt aatcatacaa
     4501 gagtaaggca acacagagaa gagaataccc tcattttgca tttgcttcaa tgcctgctca
     4561 atgaaaagat attctttgta atccgttttc tttttaggga atggtggatt cattaacacc
     4621 ttactaactg gtgggaccct gtggggtgta tctttcgata aatactctgc ttgaatgatg
     4681 ccattcctgc tttttacatt gagccacttt gcaaaacaat ttccatctac aatgttgttt
     4741 ttcccatcac ctctaaatat catgtttact aacgccaatg aaacaaccgg atcttgttcc
     4801 tccactccat ataaaccata ttttttaaac ttgtcaaact cttccgtcgt tgcttttttc
     4861 ttcacttcat cgaatgccgc aacaagaaac cctccggttc cacatgtcgg gtcatacact
     4921 aaatcatttg cttgaatatc taatacttgc gctgcaaact ttgtgatgtg tcgtggagtc
     4981 aaaacgattc cgatttcttt tgccccattg ccatacttga gaaacacttc atagaatgtc
     5041 ccaagtacat ctttgcctga ttgcatggca gagcggatgt tgagcccaag caattcttgg
     5101 atcgtgagaa caatggcttt tttaaacttg atgtggttgt cttcgctcgc aggaaggtca
     5161 atagagatga accttgaata attgggtttc ttgtgctttt ttaaaactaa ttctaccctt
     5221 gagtttatag attgaatgag aataacgggt tcttcgtcaa tattaatttc cgttccctct
     5281 acaagtgcta ataatattgc cgacattacc ctcgctctat tatctttatg gattcctcct
     5341 tcatgtagaa taccattgat ttcttcggct gtccttaaaa actccttctc attaatttcg
     5401 acttctttga tatgtgggtc ttgtgtgctt agaactttct cgatttgatt cttggaaagt
     5461 agccccgtta cctcaacatc attctcggta atggtttccc atttaccatt ttttaggaat
     5521 tgactcttcg cgacaaaacc ttcgtctcca ttgcctgcaa ttcccgttac aaacggagct
     5581 ttaattaatt tagatttgtt aatgagattt gcataatcgt cttttgcctc tttgagtgcg
     5641 atatttattt ttgctctctc gttttttgcc tcaataacat aacaagtagt ttcattaatc
     5701 tttaccatgt tttcaggttt gctcttacca agacattcgg cgattgactt tacttgtaga
     5761 cattcttgtt gtgtatatat ttggttcttc tcccaccctt ttttgtttac caattcatca
     5821 aggatataaa agtaggcatg aacctccgat tgagaagatg aacgtctttt agtcatatca
     5881 tccccctgtg agaattttgt acattaaacg tccttcacat tcgcgaatgt cgcctgtgat
     5941 atagataacc gtggaaagct aaagctttcc gctacatttt tctgagtcga ttttaaagat
     6001 tatatggcac tcctagctct tgacccagct gctttaaggt ctccaccacc ttgtcattta
     6061 gaggaagacc attcttctta tgctcctccc atttctcgaa ctccttttcg ccatggatat
     6121 agacccggct ctgccctttt gccttctggg agttcttcag ttgctggatc atactgtcca
     6181 tagtggactt gaacttgtcc agctcaataa agttttcgat cttgactgct ccaaagaaat
     6241 ggcccacgtt gacaaaggtc ttggttcctc ttttcttgga tttaacatag gggccgaatt
     6301 cagctccgga caagacaccg gataatatat ccaccaataa agacaatcca taacctttat
     6361 gaccacttaa tagttcgcct tctccaccta aaggcaatat ccctcctcca gcagtattag
     6421 tcatattgtc aatcaccagt ttggtatcag tagtaggaag gccctcctgg tcaactgccc
     6481 atcccagagg gacgggcttt tcctgccgaa gatagacttc gaattttcca cgaggcacaa
     6541 cgctggtggc catatccaaa acatagggtc tttccttgcc agctggaatg gctacgctta
     6601 tcggattggt tccaattatc atatctttgc tgaaagttgg aactaccaaa ggttcagaat
     6661 tggtcaatga aataccgatt aagtcgtgct ttaaagccat cagggcataa tatccggcaa
     6721 ttccataatg attgctgtta catactgtgg caaatcccaa gccgttcttt ttggcttttt
     6781 ctatggctaa attcatggca aaaaatccag ccggttgccc caacccattt tctccgctga
     6841 cagccacagt gactggcgtc tccttcaata tcttgggctt tacgttggga aggataatcc
     6901 caccctttat tccatctaca tatcttttta gacgagcaac cccgtgtgag gatatgcctc
     6961 tcaaatctga ggtgaccaga ttatctgcaa ctatcttggc atctgcttct gacaccccta
     7021 tctttttgaa gatgtcaacg cagaaattag tcaacttaac atcatttacc actggcatag
     7081 caactccttt gtttaaggtt tccgttatat tctataataa ggataaatca gcccagcctg
     7141 gggcataaca taagctttat agtcttcgcc gtaccattcc ctgactgatt ttacagcatc
     7201 atctaaagtc tgagcgtgat gatagaatct cttctccaaa atctcctttt cgattttgga
     7261 tacgaccaat attttgaatt tctttaagac cttggcattt cccactgccg tgtgcccacc
     7321 cacagttatc tttttagggt cggttcttaa aagcccatcc aagtccttgg cttcataaag
     7381 ccactggccg aaagtgtctg aaccaatacc ctcccggcat tcagcaacca gaacaatggt
     7441 tccgccctcc ttcaacacca gcttggcatt gtttatggct ttctgagatt gatataaact
     7501 tacatctttg ggataaccac cggaacaagc aaatacgaca tctgctaatt tgttaacttc
     7561 gactttatag atgtttctgc aagcctccgc gcccgagtca aaagctttcc ttaggtcccc
     7621 tgccaatatt ttgactatct gttttttatc gtttatcaca acattcacaa taaagttaac
     7681 ttttgctttc cctgctgcct catccatctc atcgcttatc ggatttccct tggttttagc
     7741 aatatcaact tgtggatgaa ttatcatagc atgattggct cggatggtct catatccagc
     7801 tatgccagga agtatccctt ttctccctcc ggaaaagccg ccaaaataat gggtggttat
     7861 catcccagtc aatattttaa catccgattc cacccaaagt ctgtccaccc atagttggtt
     7921 tccagttgaa agttttccca aggagatatt gtttgctctg caatcgtggc ttattacttc
     7981 aaaattcttt acaatctcct ctccgaaatt atctatattt tctttttggg tatgaggacg
     8041 gtgagcacct gttccaacca gaaattttat gtccttgtcc tttacccctt cagctcttaa
     8101 ttcatccaca atcggaagca acatattata actttccctc ttgtagggga ctccacgggt
     8161 ataatctgcc acaacaatac agacggtctt cttcccttta gctatctggg aaagcttctt
     8221 tgtgccaatg ggattatcca gtgcttgcct tagacctaaa ttcacatcag gtaaacccgg
     8281 agcttctttt aacttcagga taccaagtaa attcttttca tccagattca aatccagatg
     8341 agaatcacca tatttgagtt caattttcat atctttttaa ggtttgaggt tgatttaaac
     8401 tacaaaaaat ctaatctcca taagcttcag ccacaagctc aattatgttt tttatcttgg
     8461 ttttcatttt cccttgtaaa aaggtttcct cgaaacgcat catgcatccc ggacaactgg
     8521 tgaccaagaa atcaggctca attttttgta tctccttaag tttatactcc attatcgctt
     8581 ccgaagttgg gtaatatttt agatggaagg agccaccccc accacagcat ctatccgcat
     8641 ttttcatatc aataaactta agaccaggaa gcatcttcaa aatctttctg ggttgagatt
     8701 ttatcccctg gccacgatta agatggcagg ggtcgtgata aaccactgtg taattcagct
     8761 cttttttgaa atcttttttc atttcgattt tatcaatcag gaattcattt atgtcatata
     8821 ccggaatatc gaaatctact cctaatagtt ccttatattc ctttttaagg gttaagccac
     8881 aggaagcgca attaaccact atacagtcaa ctttagcatc cttgaaaacc tttaaatttc
     8941 ttttggcgtg cttgattgcg gtcttaaaat cgcctgaatt gtaaatggat gttccacaac
     9001 agatctgctt tttgggaata actacctcaa tatcgttgtg ggtcaatacc tttatcatcg
     9061 cctcacccac ctggggataa accagatttg ttccgcagcc atagaaatat cccactctca
     9121 ttttgggatt tttagctttg acaacttcgg gaatcctctt tcttagaggt ttcctgctaa
     9181 tcttgggata tatcctttgt ttccatcctt gaggcaaggg caaaaaaaga tttattgggc
     9241 ttttttgggg caaaaccttc tgagctaaac tacccataaa cactgcaaag cgtccaactg
     9301 gtggtaaaag ccgtcctctt ttcaaaagat gccggaatat caatctttcc acaaaggtca
     9361 attttcctct gttcaccagt tcagcccgag cagccaggat tatgtcatcc acccggacac
     9421 cacccggaca agcttcaacg caggatttac agttcaggca gaagtttatt ttttttctga
     9481 ataggtcggt cagatccaga ttcccctcga aaacctgctg gattagagcc atcctcccac
     9541 gggctgaagc cggctcatac ttcaattccg cataaatcgg acagaccgaa aggcaagtgc
     9601 cacatttgag gcatttaagg agctggtcct ggtgtttgga caagtatttg aaatctggct
     9661 gtgtttgagg cataatatct tttcaacata aaaatcttat acctctttgt caagccaaaa
     9721 tcgctatagt tgagttgtca tacaagtata gtacttactg tatattaatg gccgcgtgaa
     9781 gaacgccgca ggaatcagaa taggggatgg ggagactttg acttaatctt ttgatttcca
     9841 aatgaagaga gttccaaaga acagggatgt ttgcacttag cggttctttg tgttaaggct
     9901 tctttacttt ttcctttttg ggagtttctt ttactgtctc ttccttgatt gcccttaaag
     9961 ttataatttt ctccggttcg ctttccattc ttacctcctt gttgtctttt atctcagatt
    10021 tctgagcagt aaattgtatg ctctcgccgt cttctgacca ctggaaatca tagatattaa
    10081 tattttcatc ccaacggaaa cctccgtctt tgattgcccc ccaccagttg cttatgtatg
    10141 gctcttgagt tttttgcctt aaactaactg tctcaacaac aaactctcca ctaggagcaa
    10201 cgaaagtata agcaaaatgt ctcgagtttg gtgaccagag aatttgatcg atatcgctat
    10261 caaatagcca ggatataaca tctatcttct tgctctttaa cccgaaaaga ccaacccagt
    10321 tatgaactag cccgcaggaa aaaactatct tcttgccatc tggtgataag gaagccagat
    10381 taaacctagt atctgggaat cgcgcaccct ccggatgatg aatatgagga ggaaattctt
    10441 ttgggagatc ctcaattctt aaaattggct tcgaattccc atctatatat acagcagtag
    10501 actggtcttc tttgatgtaa gctcttggag tgattttctc tttgactttc ttggtggctg
    10561 tatagctaca atcagtaaac aaaatcatgc atattactat ggaagcgaga acaataacac
    10621 ttcctagata actaatcttc ctatacataa gacccccttt ccattaatag ataaaaatac
    10681 gccttggagg agtcgaacct ccgacccgct gattaagagt cagctgctct accaactgag
    10741 ctaaaggcgc aggtttaagg cagttcacgg ttcacggtag atagttcacg gtaaagctgt
    10801 aaactgtgaa caatgaacgg tgaaccgtga acagaattaa atgagggtgg atgggatcga
    10861 accatcggcc ccctgcttaa aaggcagatg ctctaccact gagctacacc cccataagaa
    10921 agttatacaa aaatatcaaa aaatgaatat atgtcaagac aaaataggtt aaatcagaat
    10981 agtttcgttt ctcttacagc cggtggatat caacatcacc ttaaccccca actgctgaga
    11041 gatgaaattg atataatctt ttgccctctg aggcaaattt tcgtatttgg tcattccgtg
    11101 agtgctcgtt ttccatccat ctatttcttt ataaaccggg gtacagtttt ccaaaaactt
    11161 caaatcattg gtgaagttct caattgtttt gcttttatat ttataagtaa tacagacctt
    11221 aattttgccc aagtcatcca gaacatctaa ttttgtaaga gcgattttgg ttatgccatt
    11281 aactctcacg gaatacttaa gtaaaaccaa gtccagccag ccacatcttc tcggtctgcc
    11341 ggtggttgct ccaaattcct tccctttttc ccttatggct tcgcataagc cgtcatcgaa
    11401 ttcagttgga aagggtccat ttcctactcg ggtggtatag gttttaacaa ctccaataac
    11461 ttcgtcaatt aaggttggtc ccacgcctgt accggtgcaa gcacctccag ctgagacatt
    11521 tgaggaagtg cagtagggat aagtcccatg gtcgatatcc agcattgcgc cttgtgcccc
    11581 ttcaaacaga accgatttgc cgtttcgtat agcttcgttc aaaatgagcg agacatcagc
    11641 cataagcgaa aggacttgct ttttatattt caaaataact ccactctcag ttttcaaaga
    11701 ggaaaattcg cctcttttta gttttctaaa aagatgggaa ttatttttta agttactttc
    11761 cacttttttc ttgaaaactt caggatataa aatatccctc aatctgattc cgcatcgacc
    11821 tattttatct ctgtaagctg gacctatccc ttttccagta gttcctacct tgtttctgcc
    11881 ttttgattcc tcttctaatc tttcaatcgt tttgtgataa ggcataacca aatgagcgtt
    11941 ttcactgatg aatagacgat tcttaacatc tatacctctt tttttcaaat cagctatctc
    12001 gaagaataat tgctccaggt caatcaccat tccgtttccg atgatgcata tcttgtgcgg
    12061 atggagtatg ccggttggaa tcaaatgcag gatgaacaaa ttatcaccta tcactaccgt
    12121 gtgtccagca ttagctccgc cttgaggacg cgcaatgata tccgcctttt cagtcagtag
    12181 gtccaccact tttcctttcc cttcatctcc ccattgcgtt cccactataa cccaattggg
    12241 cataaactct ccttgatttg accaaaataa aggctaaagc gtaaagccct ttagcctaaa
    12301 gatggttttt taaagaatcc tacttttttc ttttctcgaa gatctcgtca attaagccgt
    12361 aagctttggc ttcctctgcg gacataaaga agtttcggtc agtatccttt tcgatcttct
    12421 ccaaaggctg tcccgtatgt ttagtcaata tcctgtttaa catctcgcga gtttttatca
    12481 tttccttggt ttggatctca atatcggtta cctgtccttg cactcctccc cagggttggt
    12541 gaatcataat acgggaattg ggcagggcgg aacgttttcc tttggttccg gcagtcagaa
    12601 gcacagcccc catgctggct gctataccca cgcagatggt tgccacgtct ggtttaacga
    12661 actggatagt gtcgtaaatg gccagcccgg cagtaactat ccctcccgga cagtgaatgt
    12721 agatattgat atctttttcc ggctcatccg cttctaaaaa gagcatctga gcgataaccg
    12781 agttggctaa attgtcgtct atgggttgtc caataaagat tatcctatcc tttaaaagac
    12841 gggagtaaat atcataagct ctctcccctc gggtggtttg ttcgatgaca tagggtatta
    12901 aagtcatttt gttttttctc cttgagctgg ttttatcttc tcttctgtta tttcggcttt
    12961 tcccagaaga tagtttaaga ctttctcctc caggatagtg tcttttatat tctctatttc
    13021 tctatttttg gctaaaaact ctttagcttt atttatttcc aagttataat ttttggcaaa
    13081 tgctttagtc cattcctcta tctcctgggg ctcggtatcc attttttcct tctcagccaa
    13141 acgatgataa atgagattcc atcttattcg atctgttcct atgctcctat actgttccct
    13201 gattttctcc tcgtccactt tgtcgtattg ttttttgaaa tcgttcaccg ctgcatccag
    13261 atacctctgg accatagacc ccgggacctc aaaggagttg acctcaacta tcttttggat
    13321 aaggtcgctt tttaatctgg tttcgctatc ttctttggcc tttctttcca agtcctcttt
    13381 tattctgttc ttgagttggt tcaaatcttg atagtctccc aacgatttag cgaagtcatc
    13441 attcagctca ggcaaaacct tctctttcac ctctttgacc tctatattga aatgagcctt
    13501 cttgccggct aacttctttt caaaataatc ttgagcgtag gttagttcta tctttttttg
    13561 gtctttggct ttggttttct ccaacccctc ttggaattct ttaaagatat tttgtgagcc
    13621 caattgaacc aactgattgg tgaatttctc cggctttttc ttttggtcat ccaacattgt
    13681 cctctccaaa tccaagacta agaaatcctc gctttttgat tccctttcaa ctgctctgag
    13741 ttgagcgttc ttctccctga gatagtctaa ggctctctcc acctcctttt cagttatttc
    13801 agaaaccttt cgcaccaact tgactccctg gtaatccttc ggttcaaatt ccggtatgac
    13861 ctcaagttca gcctttacct ttaagggctt accatagtca aaatccaact ccttgacaat
    13921 cgggacggtg ataggagaaa ggttagtttg ttttatggca tcttgaaata tcttagggat
    13981 gagagaatcc agaacatccc tggcaatagc atccttgtac ttttccttga cgatttctag
    14041 gggagctttg ccaggcctaa atccctttat cttagctttt tttctatact tttcctggaa
    14101 gagatcaaac tgctcatcca ccttttcagc tggaagctct atctctaatg tcctcttcca
    14161 gcccctgtcc tctgttagat taactttcaa ttctcctcct taattggtgc gaaagggggg
    14221 agtcgaaccc ccacccgata aaggactgga tcctaagtcc agcgcgtctg ccagttccgc
    14281 cactttcgct ttttgacgca acttctaaat ataaaaaact aagcataggt gtcaagctaa
    14341 taattaagtt ccaaaaggaa aaggtccaat ctttcctctt gacaattgtt tcttatcttc
    14401 ttaattgtag ttgttaaatt tttcacaaat ttaaatgaaa agttgaaatg aaaagaatat
    14461 cgctatccac catttatcgg ctttccattt atcatcgctc cttgcagaga agagaagaga
    14521 gaggcatcca aaccatttcc tctaaggagt tagccagaga ggagaagctg actccggctc
    14581 aggtgaggaa agacctttcc ttctttggga gttttggcac aaggggattg ggatattcgg
    14641 tcaaacagct gaaagataac atagcaggaa ttttgggcat taaccgacag tggaatgtgg
    14701 ctctggttgg agtgggaaat gtcggctcat ctttggtttc ctataaggaa tttcctaagc
    14761 aaggtttcaa cataaaattg atatttgaca atgaccagcg taaaattaat aaatatcaca
    14821 aggaaatctt agtctcggat gcgcgagaac ttaaaactaa actcaaagaa agcagcatag
    14881 atatagtcat tatagctgtt ccagccagtg tcgctcaaga ggtggtcaat caagtggttg
    14941 aagccggagt gaaagccatc ctgaactttg ctcccaccaa tgtagtcgtt ccagaaggag
    15001 ttattatccg ctcagtcaat atggcaatcg agcttgagca cctctctttt gctttagtaa
    15061 ataaatcact attcaaggct tgagataatt tcttgtattg taacagatta gggcaactaa
    15121 atatattagt ttggtgaaga aattcccaga aatgcttttt gggaggttac aaattagtac
    15181 tcttaatttc tcatgccctg gaaaacccga aataaaaagt taaaaaaacc tcattttttt
    15241 gttgacaaac tatttgacat cgtttatcat aagactggga aagaccgtat aagagtagtg
    15301 tagtggcgtt tgctctctta aggaggtggt attttacttt tttcctaacg gtcacgaagc
    15361 aatgtaagat agaaaacaga ttaaacaaaa accataacaa aggagtggtg cgatgaagag
    15421 aaagcttttg gtcttggtgt tgggaataag tctgtttgtc ttgctgggtt taagttttgc
    15481 tttagctggt gactacggag aaccggatac tgtctggctt gagaaaattc agactgtaaa
    15541 cccgaattca caagtagtct atagaattta tctgttcaat gatcataaca tatcggcgtt
    15601 taccatcccc attacattcc ccgacacaac cagcaatatg gatattagcc ttgactctgt
    15661 gagctccgta ggaacaagaa ttgctttagc tcagggcaaa aacgacccaa gcttcttccc
    15721 cacggatagt actaatgtgg ttaacaaaaa cagaatagcc ctgtggatgg tttggagctg
    15781 ggtgggggca ggataccttt ctcctgttac tcgtacgcag gctttagcaa ctcctgtagt
    15841 taagatatat tttagaaccg gcccttcttg ggtttcacca aactatgtat cggttgatac
    15901 gaccactatg gtgtctggtt cattactcga gactgattca gttggagtcg aattcgtccc
    15961 caaacttgcc ggcaagggtg cgttagatgt gaccgatatt actacaccta atctacccat
    16021 ggtcttcagc ttatctcaga actatcctaa tcccttcaac ccatcaacta ccataaggtt
    16081 taccttgcca aaggatgatg aggttacttt gatgatttat aatgtcttgg gacagaaggt
    16141 aaaggcttta attgaccacc aagatctacc cgcgggaacc aaacaagtgg aatggaatgg
    16201 cacagatgct aataacaacc cagttgccag tggcacctat ttctatcaga taaccaccga
    16261 gaataacaga attactaaga agatggtatt gacaaagtaa gtgcaaaact cattcaagct
    16321 ttccaaatcc ctccccgatg aactggggag ggatttttgt ttgcttaagt tttggtctta
    16381 agacatgtaa tggattacga ttcaattggt tacacttcat ttgatttcaa tcccaaaaga
    16441 aaattgaatt ggaaattcgg gaaattcatt atctgcttat aaatcaacga attaccgaga
    16501 taatattctt caaattcaaa atatcttaaa aaattcccct aattcgttaa tttttgttga
    16561 caaatcaaga attataggtt agaattcaga gaaaaattaa cagaaaatca attacttaat
    16621 cactctgttt gtttcattat aagtcattat agtgattgaa cttaaaaaga ggataagatg
    16681 ttaaaaatag aaaatggcaa attattgatt ggaagagagg aatattatcc catatcagcg
    16741 gaactgcatt attttagagt taataagaaa tactggtcag tatgctttga aagaatcaaa
    16801 aaggcgggat ttagaataat ctccacttgt attccttgga acttgcatga gatttcctta
    16861 ggggatttcg atttttttgg ggagacagac ccgcgccggg atttgattgt tttcttggag
    16921 ttggctcgcg aatttggttt taaagtcatc ttaaaaccag gcccctatat cggagcagag
    16981 tgggataatg gaggatatcc tgattttctt ttcaacaacc ctgagtttct ggcaaaagat
    17041 tctcaagggc aaagcgtgga acaggcaagc caggttaagg ttaaatcagg gttagttcct
    17101 tgttacaatc atcctcgctt tcaaaatcag gtcaagaggt atttcagtgc cctttcggat
    17161 gtgattaaag gctacaccta ccccaagggc ccaatcatta tgattctatt agataatcat
    17221 ccttcttttg gcagaaatat ggagccattc aaattggatt ataacgagta tgtggttggc
    17281 acactttacc ctcagttcct acaggaaaaa tataaccaga taaaacatct gaataacgtt
    17341 tacgttgaga aaagtaaacg ctttacagat gtccagcccc ccaaacactt gaaaataaat
    17401 caacccaaac agctcctcaa gtatctggac tggatagaat tcaaggaaaa atatctggca
    17461 aattacattg aaaacttgaa ggagatattt gtctcctgtg agatacctgc aattttctcc
    17521 acagttcttt ttcaggaaaa actcttttca gttccattca attggacttt gatagaccgg
    17581 gaggaaaaat acgtaggagc tcaggtggga atggagaaaa actaccctga cttaattaac
    17641 catctccgtt attacgctac ctgctcaaga tttgcctggg ctaacgagtt cagagtaggg
    17701 aattgttcgg ataacccctc ggacagccgc aaatattttc cagtctcctc aagggagacc
    17761 aaacttcaaa tggtaagttc gctttcaagt ggaattaagg gatataatta ctatatgttc
    17821 gtggaaaggg accattggta tgattcaccc ttagctgagg atggaacact acaaacaagt
    17881 tatgagctca taagaaagtt caatcaagca gttgaaaaaa tagaattgga gaagttttac
    17941 tctttagctg aaataggatt ggttaattac cgcccctacc tgtggcatac acatctcgga
    18001 atcgaaaaac catacggata tattctaaac ttagttaatc taaccagcaa aaacctcgct
    18061 tctgaccttt tgacactcaa tctcgatttc ggcatagcag atatgtggtt ggcggatagt
    18121 ctgagtaaat tcaagctcct ctttatgccg gtagcagaat atatggacgc tgatggccaa
    18181 aagaatatcc tggagctggc taaagcagga aagagtgtga tcctgtttgg acttctgccc
    18241 aaatatgatg aacggatgaa gggttgcgag atactgtcca agggtttgag attgagaaca
    18301 aaccagaaag agcagatagg agagataaag acttcgtctg aagaattcat tactcaagct
    18361 tatggtttta ttcggagagt gggaggaaag tcccaggtga tagccgaatt ggataataag
    18421 ccagttggtg taaagctgaa ggtgggtaag ggaatggttt atttcttcag tttcgatatc
    18481 tgtgccagat tggaccattc caagttatct ttcttaagga gcgtaatcga cgattgctca
    18541 atttcgagcc cgattgttac caatcatccc tttacttatg ttgttccgca taaagccaac
    18601 aaaaaagtgg tgctgtatgt agttcgctgg gacaaaggtg aagaggagaa ggtgataatt
    18661 caggtggact gcagaaaagt ggggattaag ggagcaagga ttcgtctgat agatttattg
    18721 ggcgaggaag tgatcaagac caccagttcg gagttgaaaa gaggtttagc atttaagtta
    18781 ggtcctttag atagtaagat gtatctggtt gaaacaagat agctactgtt tttcttctcc
    18841 tgtatttgaa agcaggacaa agcaggagct tttccccctt ttgaccgtaa gcattatctc
    18901 ctttttggac ttgaactggt ccagtatttc tttaaatccc ttcaaatcag ttacatcctt
    18961 atcttccact tttcgaatta catccgctgc gaggagttct gccttgccag ctgacccttc
    19021 aacttctaca aaggagacta atacaccggc tctgtcctcc aacatatttt ggcgataaac
    19081 gcagattttg cattagcaaa atctgcctcc cgagcccctc ttcttacatt ctgtaggaat
    19141 gtagaggggt cgggagactc aatcctcgtc ccgccagggc gggacagagg ggatagggtc
    19201 ggctgccccc tcttcttgct ttcgcaagaa agcagagggg gcaagaacgg caaggtaatc
    19261 cccgaacttg ggataataat aagttacgac accttctgct ttctaacgca aattttggga
    19321 taaatcaagt ccgtaatttc tttgacagtg aatccgagaa aagtttcgta ttcctcagct
    19381 ttcacttttg gctgtttgcc taaagtgata ctgaggttct tagtcccggc atctcgccag
    19441 agcttgagtt tgaccttttc tcccacctgg ttttgagaga agaagggtga acttgtttaa
    19501 atcctccctt ttttctgcga atatcttatt tacctgatat tgcaagatta tgtctttcga
    19561 tttgatccct gcaatgtcag ctggagaacc agtctgcacg tcgcttacca gtatcccttc
    19621 aagttgtgtc tctctgaaac atttggcata gtcacagctg agtggctgga cgaatacgcc
    19681 gagtcaccgt cggtcaattg tggctgagat caaaagttta tctttcacat cgttggcaat
    19741 gcttatcggt atggtaaacc cttgccccct tccaaaccct atggaattga ttcctaacac
    19801 ttcaccttta agatcgacca aaggccctcc ggaagagccg gggtcgatgg ctgcatcaat
    19861 ttgtatgaac ttattgatta attagccttc caaggttaag tcagcaagaa cccttccttt
    19921 tccacttact attccaaagg aaaccgttcg gtcaaatccg tatggattcc caacagctat
    19981 gagccactct ccgacttcaa cctggtcaga gtttcccatt attggaacta aaagggtttc
    20041 aggagtttca atcttaataa gggctaagtc ggtctgtttg tcagtgccaa ttatttcagc
    20101 tggatattcg agtttactta gcagagtcac ggttacagat aaggcatcgt ctaccacatg
    20161 ttgattggtg aatatataac cccttttgtc cactatcaac ccggatgcca gcactttata
    20221 cttgaaatca ttggatttct ttactacgtc aatgtgtact accgaagccc taatgccatc
    20281 tgaaatcgac actatcagat tttgcgagcc atcaaatagt tctataaccc gttgtttgtc
    20341 aatgtcttgg ggatttatgt tagttgcatc aaagattatg gaagaaaaca agagtatcat
    20401 tattcttttt gtcataatat cctcctgaga caagctaaaa tcatataaac aaattcccca
    20461 gtagaaagca agaaagaaac aataaattgc aacggatcag acgatagttc gtataacatt
    20521 gcaggaagtg gcagttgagg gatgattttt gtagtgtgtt gggcagattt tccttgacaa
    20581 aataaaagaa tttcttatat tcaaagtctc aagtaagtca tgcaaaacag tgtctaaaat
    20641 accgccttta agcaaaagtt caacccaaaa aactcttttg ctttttgttt tttccgctta
    20701 acatatcagt tttactggtt tcaatatcaa gggaggaatg ataagaattg gctaaacaac
    20761 ccaaaaggta tcgtgatcag gataggtcgc tggaccttta tttgagagaa attggtgaga
    20821 ctccgcttat caacgcaggc gaagaggtgg agttagccaa aagaattcgc aagggagatc
    20881 aggcagcttt agataagctg actcgggcga acttgcgatt tgtggtcagt gtggctaaac
    20941 agtatcagaa tcaaggcctg tctctagctg acctgataaa tgagggaaat atcggattaa
    21001 tcaaagcggc caaaaggttc gatgagacca gggggttcaa gttcatctcc tacgcggtgt
    21061 ggtggataag gcaagctatt ttgcaagccc tggctgaaca atcgcgcatt gtcaggcttc
    21121 ctttgaatcg agttgggacg ttgcataaga tagggaagat ctccagctcc ctggagcagg
    21181 aatttggccg agagcccagc ccggacgaga tagccaagaa actggaatta actgaaatgg
    21241 aagtcgcaga tacactcaag atatccaata gtcacctgtc gctggatgcc cctttttcag
    21301 tttcagaaga taatagtctg atggatgttc tggaggacga gtttcaaccc tcaccggatg
    21361 aagccctttt ggatgaatcc ttgaaactgg agatcgagcg cgctttggat agtttaaccc
    21421 cgagggaagc tgaagtgata aatctctatt ttgggttaaa ccatgagaaa gccctgacct
    21481 tagaggagat tggggcgcgc tttggtttga cccgagaaag agtaaggcag attaaggaaa
    21541 aagctattcg ccgattaaag catgcttccc gaagcaggtc cttgagggct tatctgagtt
    21601 aataaaagat taggtgaacg accaaggagg aaggttttga tttggaacct tcctccttta
    21661 ttttttgttg acaaaaaaaa gaggtttcgt attatttaga caattgtata aatatacaac
    21721 tatataatct tggaaaaagc gatggctata gatgaggtat ttaaggctct ggctgatcca
    21781 actcgaagaa aaatactaga ccttcttaaa aagaatgacc tgaccgcagg ccagatagca
    21841 gaacatttcg agatatccaa accctcaatt tcccatcatc tatccgtctt gaaaggagcg
    21901 gacttggtga ggacagagag ggatgggcag tttctggttt gctccatcaa cctttcggta
    21961 atggaagagg tggcggagat attttttgac ctatttaaaa agaaaggagc aaaatcaaaa
    22021 agtttttcaa agaagaggga tttcccctgc tcgtaatttt gatttcgata attatggcta
    22081 ttttctttta tcaaatctta ccgcagagat ttgctattca ctggggtgca gacggcaatc
    22141 cgaatggttt tgccaacaaa tccttctggt cggtttcctt tggtcctttt acgattatcg
    22201 ccatttactt gctcttctta atcataccca agattgaccc ccgaaaggag aatatagcca
    22261 agttcaagca ataccactta ttcagaaacg tgctcttagt tttctttctg ttctttgagt
    22321 atttgacttt gcgaaccgca gcttcaccaa atcagaagct ctccagctct tatatcatct
    22381 ttgggctggg gctgttgttc attttcatag ggaactacct acccaagatg agctccaatt
    22441 tctttgtggg gattcggact ccctggactc tatcttcgga cgaagtctgg aaggaaactc
    22501 atcgaatagg cggaaagctt ttcgctttga tgggaatatt atttctcttg ggcagttttc
    22561 tgccgaaagc ggtctcgtgg gttttattta ttccactcgt cggatgcata tttttcatag
    22621 ttatatattc ttattacctt tataagaaat caaaaataaa acaagcacaa ggataaatca
    22681 tatttaaaca aaggaggaca gaatgaagtt tactaagtgg gtaatgtttg cggccttact
    22741 tctcagcatg tttttcttgt cagaaaagtc tttttcagac cagaccttct tggtgaaact
    22801 ggatggcaag aaagtgggtg atgagatatt ctcctacaga acagacaaga aagatacaaa
    22861 cgtggttatc tttagctcta aaaccaaaat cagcatggag ggaatgggag tatcaattga
    22921 gcaagaggaa aaactgaata agagaaatta tgctttcagc gaatatcaac tgaaggcgga
    22981 tatggccgga caatatcagg aaatggaagc caaagtggag ggtgatagca taaaagccag
    23041 cgcattcatc aatatgcagg aatacgaaaa gaaagttcct tatcgggaga gtgatgtagt
    23101 attgggatta ccaaccctct cagtttattt ttatcaaatt ttgctgaaca gatatgattt
    23161 caacaagaaa gggacacagc agtttcatgc ttatcttccg gataaactat ctgagggtcc
    23221 tttgaaagta actctaaaag agaaatatcc tgccaagctc aaagaggagg gatttcaagt
    23281 tgaccatttg gaagcggttc tggtgaattt agtgataaac atatgggcca aatcagataa
    23341 ccatcagttg ctcaaagttg atattccttt tatgaagttt gagtcttctc tgccagaact
    23401 ttccataata gggaaagaag aagttaagac cccagatatt ataagcgcca ataattttgt
    23461 ggaaaaagaa attaccttca agtccggaga attcgaaata tcagggacac tatctttacc
    23521 taaagatata aagggaaaat ttccagcggt cattttagtt caaggctcag gtccgcaaga
    23581 cagggatgag actgttggtc cgaacaagcc cttcaaagac attgcccatg ctttgtccaa
    23641 ttccggcttt gcagttttaa gatatgataa gaggacttac acttacaagc gtccgaaatt
    23701 ggatctgact aatataacct taaaagaaga agtgatagat gacgccatct ggggtattaa
    23761 attcttaagg gagcaggcag aagttattcc tcaaaagata ttcgtgttgg gtcatagttt
    23821 gggggcggag tgcaccccct tgatcgcttt agaggataaa aatcttgccg gggtgattat
    23881 gctggcacca tctgccagac cggcggacag tttaatctta gagcagatag agtttcaggc
    23941 aaaggttgca ggaccgtcag ccagccagga ggcaacgaaa caattagaag aattaaagaa
    24001 aggttttgcg caaataagga ataagacttt tccagaaaat cagatgcttt tcttcaccag
    24061 tggaaaatat tggtattatt ggttaagtta tagcgctgcg gacacaatta aaaaaataga
    24121 ctgcccgctt ctgcttttgc agggtgaaaa ggattgtcaa gtgagcatga aagattttga
    24181 aatatggaat ggggtattaa agggaagggg aaatagtacg ctaaaaagtt ttgctaatct
    24241 caatcatctc tttatgtatg tggaagggaa atcaactggc gaggagtacc aaagggctgg
    24301 tcatatagat ggggaagttc tcaaagctct gattgaatgg attaagaaat attctatgtg
    24361 agcccgccat ccttgagtct atcgatttat ctggcattgc atataaaaaa ggagagtcaa
    24421 aaataaagac tctccttttt tatatgattt gctataagtt atttgttgtt tggttggctg
    24481 caggaattgt cttgtgtttc ctttggcttt tcctctggct tggcatcttt tggtcttgta
    24541 ccgaatcggg tcaaaagccc tgcccccaat ccaaccgtta ttataacccc aaatatcacc
    24601 catccgagga ttgccataat ccaacccaaa accaccaatg gcccaccaat tactccaatc
    24661 aaccgtgcga aaagaagcac cacttccact gccaataccc ctactatcag ggtcatcatc
    24721 ggggtttcgg gcttaatgga ggtcttctct tttatcttat ttccgagaaa gtaagcccca
    24781 ctggtataag ctaatagaaa ataagctgct acggctaaag gcaacgcaat caaagccacc
    24841 gggattccaa ttatggttat gcaaagtaag acaaataaaa tcagaaatgc aggtgcaatc
    24901 aacagcaaca cagccaaacc aaccaaaaag gagatccaga agtcgtggtc aattttgttt
    24961 tttatctttt ccacattttt gggaacaatg gcgaaaacta ttatagccag aagaagaaaa
    25021 atca
//
LOCUS       DASWLN010000017         8314 bp    DNA     linear   ENV 20-FEB-2024
DEFINITION  MAG TPA_asm: Candidatus Zixiibacteriota bacterium isolate
            SMAG_U2949 k141_651067, whole genome shotgun sequence.
ACCESSION   DASWLN010000017 DASWLN010000000
VERSION     DASWLN010000017.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37960594
            Sequence Read Archive: SRR10376091
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Candidatus Zixiibacteriota bacterium (soil metagenome)
  ORGANISM  Candidatus Zixiibacteriota bacterium
            Bacteria.
REFERENCE   1  (bases 1 to 8314)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 8314)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (26-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/12/2024 08:28:26
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 1,440
            CDSs (total)                      :: 1,416
            Genes (coding)                    :: 1,412
            CDSs (with protein)               :: 1,412
            Genes (RNA)                       :: 24
            rRNAs                             :: 1 (16S)
            partial rRNAs                     :: 1 (16S)
            tRNAs                             :: 21
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 4
            CDSs (without protein)            :: 4
            Pseudo Genes (ambiguous residues) :: 0 of 4
            Pseudo Genes (frameshifted)       :: 0 of 4
            Pseudo Genes (incomplete)         :: 3 of 4
            Pseudo Genes (internal stop)      :: 1 of 4
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..8314
                     /organism="Candidatus Zixiibacteriota bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_651067"
                     /isolate="SMAG_U2949"
                     /isolation_source="soil"
                     /db_xref="taxon:2053527"
                     /environmental_sample
                     /country="USA: Oregon"
                     /lat_lon="42.99 N 123.63 W"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            complement(<1..684)
                     /locus_tag="VGB16_00535"
     CDS             complement(<1..684)
                     /locus_tag="VGB16_00535"
                     /inference="COORDINATES: protein motif:HMM:NF012738.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_process: GO:0008643 - carbohydrate transport [Evidence
                     IEA];
                     GO_process: GO:0055085 - transmembrane transport [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="carbohydrate ABC transporter permease"
                     /protein_id="HEX9916197.1"
                     /translation="MKFIKKLTLFAILLIVLAGMLFPLYWMVVSSLSSKASAMFGFSD
                     LLPKKLTLKNYIDVWSWGPFPRYFFNSIFVAVCVTLGNIIFCLMVGYAFARKNFPLKN
                     IMFLSVILVLMIPVHIIIIPTFLIIKTLGWYDTYFALIVPWLVNPLGIFLLRQYIQAL
                     PRDLEDAARVDGAGDFAILFKIVMPLCKPALAVLAIQAFLVNWNSFLFPFILTSNQNM
                     RTLPVALALY"
     gene            complement(685..1557)
                     /locus_tag="VGB16_00540"
     CDS             complement(685..1557)
                     /locus_tag="VGB16_00540"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_004101120.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0042626 - ATPase-coupled transmembrane
                     transporter activity [Evidence IEA];
                     GO_function: GO:0140359 - ABC-type transporter activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="sugar ABC transporter permease"
                     /protein_id="HEX9916198.1"
                     /translation="MKLKSSTFLFLLPWILTLLLFWLFPLIYSFILSLTKYSVISGQI
                     KWIGFSNYLQILKDSDFWTALKNTSIFVIGTIPFTTIIALALAILVNQKIPLRGLFRA
                     GFFVPSITSMVVIALIFTNLYAKNGYLSFLCQLAGLPVPENGWLFSTATALPSIMLMD
                     IWMSIGYYMLLFLAALQAIPVELYQSADIFGANGWQKFWHITLPYLRPMTLFVVLINT
                     IKSFQIFVEIFVMTKGGPLNSTLTMVYLVYDEGLHKFNMGYASAMAYCLFLIIAIFCL
                     AQLKLFSFGKGVEE"
     gene            complement(1554..2771)
                     /locus_tag="VGB16_00545"
     CDS             complement(1554..2771)
                     /locus_tag="VGB16_00545"
                     /inference="COORDINATES: protein motif:HMM:NF024808.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="extracellular solute-binding protein"
                     /protein_id="HEX9916199.1"
                     /translation="MLKCLKLLLLISLLLSACTGTEKEHRKTLTWWQFWTDPQVRPVI
                     TELTTQFEKENPNIKVEVTDLTWSDGHEKIVVAFSSNTAPDILELGSDWVPEFSYEKV
                     LADVTLEADKMKDNYLMWEPAIVNNKMFGFPWILDTRVLFYNKDLMKKAGLDSNHPPQ
                     TWNELLDACQKINKFKPHFYGFGANAFERHRLYKKFLPFLWAKGANILSADLKTCVIN
                     SPEAKEALGFYKKLCNSGIIDIQSRLDEMFTQGKIGFVISGGWLLKNIQANYPDLNFG
                     VALMPKPSENKGIPASFAGGEFLVINEKSKNKQEALKFIKFLIQKENGLALCQAIGSA
                     LPSAKGSYYQGNENLKIFQEQLNSAFSPPAHPKWVYMEETLERKIEQVMYDKKTPDQA
                     LDEAKEEIEQLLK"
     gene            3049..3819
                     /locus_tag="VGB16_00550"
     CDS             3049..3819
                     /locus_tag="VGB16_00550"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_006928681.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF72 domain-containing protein"
                     /protein_id="HEX9916200.1"
                     /translation="MIKIGTSGYSFLDWKGVFYPENIEKGKMLDFYAKHFDTVEINST
                     YYRIPHPAVFYRLTEKTPEDFEFIVKTHQDITHIRKSIETSAQNLKEAIRPLAETAKM
                     HGFLAQFPGAFRKSKPNLDYLKKCRELFSDFPLFVEFRHKSWMEEEIFDFLKNQNIGF
                     CCVDEPQLESLVPPIARATTGVGYVRFHGRNKAGWWKGMKDRYDYLYNENELRGWIEK
                     IKGLRGATEKMYLFFNNCPQGQAVKNAQVMKKLLEEAG"
     gene            3816..4241
                     /locus_tag="VGB16_00555"
     CDS             3816..4241
                     /locus_tag="VGB16_00555"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEX9916201.1"
                     /translation="MSIDKGDIEELGEAIEQLGQVVENNLKRINQKLESFDQFVGGQD
                     KGIKQELYEIKEETQEIREFLESYDARLSRIEMKLGELFKEKEGFPSDIEKLNDIKTL
                     LASLREDANDVLGHGEYVDKKIKEFENRIDDLEKDKPQG"
     gene            4252..5088
                     /gene="mtnN"
                     /locus_tag="VGB16_00560"
     CDS             4252..5088
                     /gene="mtnN"
                     /locus_tag="VGB16_00560"
                     /EC_number="3.2.2.16"
                     /EC_number="3.2.2.9"
                     /inference="COORDINATES: protein motif:HMM:TIGR01704.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008782 - adenosylhomocysteine
                     nucleosidase activity [Evidence IEA];
                     GO_function: GO:0008930 - methylthioadenosine nucleosidase
                     activity [Evidence IEA];
                     GO_process: GO:0019509 - L-methionine salvage from
                     methylthioadenosine [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="5'-methylthioadenosine/S-adenosylhomocysteine
                     nucleosidase"
                     /protein_id="HEX9916202.1"
                     /translation="MKKISILLLAVSLVFCFCGKSGFHPKVAIIYAFSTEGELLKSQT
                     KLVKKFDINGREFYLGKLKGTSVVIVNSGVGMTNSAMTTQLVIDRFYPEKIIFTGICG
                     GIDSSNKIGDVVIPEKWVTHDLGIYKKGGFFADSVGYFSHMENKVIYQMYLPVDSSLL
                     AIAQSSATKLKGSLKPIGTREPNIKIGGIGASGNSFIDQKEKRKWLKEKLDAQIVDME
                     SGAVVQVALSNNIPVIVVRSCSDLAGGSGSNTAQEELNKFFRVAADNSAMMVGEMVEA
                     LK"
     gene            5494..6213
                     /locus_tag="VGB16_00565"
     CDS             5494..6213
                     /locus_tag="VGB16_00565"
                     /inference="COORDINATES: protein motif:HMM:NF014035.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_process: GO:0006810 - transport [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="sulfite exporter TauE/SafE family protein"
                     /protein_id="HEX9916203.1"
                     /translation="MDWVVLLVVGLAIFCGFFVQTIIGFAASVVAVPIILSVCKLQDA
                     VAIMAILLFVFSFILVLKNYKEIDKKMLLEMGIGIILGIIVGVVVLRHANPIILQKGL
                     GVFVVLYALYSLNKQKTIEVFKKLGLLFGFVGGIFSGLYTTGGPVYVTYITNRLSKAS
                     NLRATIIGVMGVSNLARLPILFANNLVPFHCIKIATFVFPLFVLSLYLGHIVYPKINE
                     CLFKRIVLAVLLISGLLLIVK"
     gene            complement(6256..>8314)
                     /gene="ccsA"
                     /locus_tag="VGB16_00570"
     CDS             complement(6256..>8314)
                     /gene="ccsA"
                     /locus_tag="VGB16_00570"
                     /inference="COORDINATES: protein motif:HMM:NF027651.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0020037 - heme binding [Evidence IEA];
                     GO_process: GO:0017004 - cytochrome complex assembly
                     [Evidence IEA]"
                     /codon_start=2
                     /transl_table=11
                     /product="cytochrome c biogenesis protein CcsA"
                     /protein_id="HEX9916204.1"
                     /translation="GQKSYYFFFGFTLLATAHLFYLFLAHRFEFSYVYNYSSSDSPIF
                     YLVSSFWAGQEGTFLLWLFFGAILGLFLIKKQGVYKNHLLFFYLLIQIFLLAVLLKKS
                     PFEELPLFPLEGAGLNPLLQDFWMVIHPPIVFVGYAGFAIPFAYALSALVQNRYKDWV
                     SFALPWVSFSCLFLGAGIFIGGYWAYKVMGWGGYWGWDPVENASLVPWLVSVVLLHTM
                     LLEKYKGGAERTNLILALFSFLLVMYGTFLTRSGVLAEFSLHSFTDLGINVFLIVFMF
                     FFSFISLILVILRWRDLKNSIGEKPLLSKQSFVIMGSALILIWAVLILLGTSSPFITK
                     MFGQASQVGTSYYVKITLPLGLIIALLSSLVIFLNWKKTEFLQIFKNMFPLLVLSLIL
                     TLLSIAYLGCLKTSYTLLIFFAFLVFTGNLFYLIKRIRSKTIAWTSLLGHLGMGLMLI
                     GIVTSSGYSIADRINLSPNQTKSALGYNFTYLGFENTNERGFLKIEIEKDGKKTIARP
                     AFYYSEYNQGVMKKPYIKSNLWGDLYLAPIEYQEGPKKENFKLKEGETKKIGEYKIKF
                     VGFERTPHAQAELIKVGAILEIFKDKQKQKSIPAIVMGPIQQDELRKVQLFNTEDYLY
                     LEEIDADFKTISLSIETNEEITQPVLVLEISKKSFIWVLWLGSVIVISSLVISAINRS
                     KNR"
BASE COUNT         2684 a         1595 c         1775 g         2260 t
ORIGIN      
        1 ataaagcgcc agagccacgg gcagggttct catattttgg tttgaggtca aaataaatgg
       61 gaagaggaag gaattccaat ttactaagaa tgcctgaatg gctaaaaccg ccaaagcggg
      121 tttgcacagg ggcattacta ttttaaacaa aatagcaaaa tcaccggctc cgtcaactct
      181 ggcggcatcc tccagatccc tgggtagagc ttgaatatat tgcctcaaga gaaatatccc
      241 caagggatta accagccagg gcacaatcaa ggcaaaataa gtgtcatacc agcccagagt
      301 tttaataatc agaaaagtcg gaataatgat gatgtgaacc ggtatcatta aaaccaaaat
      361 tacagacaaa aacataatgt tctttaaagg aaaattcttt cgcgcaaaag cataacccac
      421 cattaagcaa aagatgatat ttcctaaagt tacacacact gccacaaaga ttgaattgaa
      481 gaaataacgc gggaaaggtc cccaggacca gacatcaata tagtttttca gggttaattt
      541 cttaggcaac aggtcagaaa agccaaacat tgcggatgct ttactggata gggatgagac
      601 caccatccaa tatagaggga aaagcattcc cgccagaacg attaataaga tagcaaataa
      661 agtcaacttc ttgatgaatt tcaactattc ctccacacct ttaccaaaac tgaaaagttt
      721 taactgcgcg aggcagaata tggcaattat caagaataaa caataggcca tggctgaagc
      781 atagcccata ttgaatttgt gcagaccctc atcataaacc aaatagacca tagtcaaagt
      841 ggaattcaat ggcccgcctt tggtcatcac gaatatctcc acgaagatct gaaaagattt
      901 tatagtgtta atcagaacca caaataaagt catgggtctt aaataaggta aggttatgtg
      961 ccagaacttc tgccacccgt tcgccccaaa aatatcagca gattgataca attcaacagg
     1021 tatggcttgt aaggcagcca aaaaaagtaa catataatat ccgatgctca tccagatatc
     1081 catcagcata attgagggta gggcagttgc agttgagaaa agccaaccat tttcaggcac
     1141 aggcaagcca gccaattggc ataaaaaaga tagatatcca ttcttagcat aaaggttagt
     1201 aaaaatcaaa gctatgacta ccatcgaagt gatggatggc acaaaaaacc cagcccgaaa
     1261 gagtccccgt aggggaattt tctgattaac caaaatggct aatgctaacg ctatgatggt
     1321 agtaaagggg atagtcccga ttacaaaaat tgaggtattc tttaaagcag tccagaagtc
     1381 gctatccttt agaatttgca gataatttga aaacccgatc cattttattt gcccgcttat
     1441 aactgaatat ttggttaaac ttaggataaa agagtaaata agagggaaaa gccaaaagag
     1501 gagtaaagtt aaaatccagg gaaggagaaa aagaaaagtg gaggatttaa gcttcatttt
     1561 agtagttgct caatttcctc tttcgcctca tccaaagcct ggtcaggtgt tttcttatca
     1621 tacatcacct gctcgatttt tctctcaaga gtctcctcca tataaaccca tttgggatga
     1681 gcaggtgggg agaaagcaga atttagttgt tcttgaaata ttttcagatt ttcatttccc
     1741 tgataataag agcccttagc tgaaggaagg gcgcttccaa tggcttgaca aagcgcaaga
     1801 ccattttcct tttggattaa aaactttatg aatttcaaag cttcctgctt gttcttgctc
     1861 ttctcattta taaccaaaaa ctctccaccg gcaaaagagg caggaatgcc tttgttttcg
     1921 ctcggcttgg gcatcaaagc cacaccaaaa ttcaaatccg gatagttggc ttgtatattt
     1981 ttcaataacc atcccccgga aatgacaaat ccgattttcc cttgagtaaa catttcatcc
     2041 aatctgcttt gaatatcaat tattccgcta ttgcataact tcttataaaa tcccaatgcc
     2101 tcctttgcct caggactatt tataacacag gttttcaaat cagcacttaa aatattggca
     2161 cccttagccc acaagaaagg caagaacttc ttatacaatc tatgtctttc aaaggcattg
     2221 gctccgaatc cataaaagtg gggtttgaat ttgttgatct tttggcaggc gtccaacagt
     2281 tcgttccagg tctgcggtgg gtgattagaa tcaagtccag cttttttcat caaatcctta
     2341 ttgtaaaaaa ggactctggt atctaaaatc caggggaagc caaacatttt attatttaca
     2401 atggctggct cccacatgag atagttatcc ttcatcttat ccgcttccag ggtaacatcc
     2461 gccaaaacct tctcataaga gaattccgga acccaatccg agcccagttc caatatatcc
     2521 ggagcggtat tggagctgaa agccacaact atcttctcgt gtccatctga ccaggtcagg
     2581 tcagttactt ccactttgat attcggattt tccttttcga attgagtggt tagttcggtt
     2641 ataaccggcc ttacttgtgg gtcggtccag aactgccacc aggtcaaagt cttcctatgt
     2701 tctttttcag ttccggtaca ggcagatagt aatagggata tgagtagcag taattttaag
     2761 cacttcaaca tatttgatta ctaattaact cgaactaatt tttgatgtca agcaaaaagg
     2821 atattttgac agtattaggg atgagagcgg ttgtttcctg tacgcagggc taaatgccct
     2881 gcgctacgaa gattatatat ccgtaggggc aattcatgaa ttgcccctac ttttatcttt
     2941 gttgtaggga cgggaattca tcccgtcccc aaagaaatct atatttggct tgaccttggg
     3001 tgttcgagat gataatttat agtttaaaag tcaatcggta taggaacaat gattaaaatc
     3061 ggcacatccg gttacagctt tctagattgg aagggggttt tctatccgga aaatatagaa
     3121 aaagggaaaa tgcttgattt ctatgccaag catttcgaca cggtggagat aaattcaact
     3181 tattatcgta tcccgcaccc cgctgttttc tatagattga ctgaaaaaac tccagaggac
     3241 tttgaattca tcgttaagac ccaccaggat atcacgcata tccgaaaaag tatcgaaact
     3301 tcagcccaga atctaaagga ggcgattcgt cctttagcag agaccgctaa aatgcacggc
     3361 tttttagccc agtttcccgg ggcattcaga aagagcaaac caaatctgga ttatcttaaa
     3421 aaatgcaggg aactattcag tgattttccg ttgtttgttg aattcaggca taaaagctgg
     3481 atggaggagg agatttttga ttttttgaaa aatcaaaata tcggtttctg ttgtgtggat
     3541 gaaccgcaat tggaaagctt agtgccacca attgccagag ccacaaccgg agtgggatat
     3601 gtccgttttc acggaaggaa taaagccggc tggtggaaag ggatgaaaga taggtacgat
     3661 tatctttata atgagaatga gttaagagga tggatagaga agataaaagg acttagggga
     3721 gcaactgaaa aaatgtatct tttcttcaat aattgtccac aaggacaggc agtcaaaaat
     3781 gcgcaagtta tgaaaaagct tttagaggag gcaggatgag catagataaa ggagatatag
     3841 aggagttagg agaggcgatt gaacagttag gacaggttgt ggaaaataac ctaaagagga
     3901 taaaccaaaa actggagagc ttcgaccagt tcgtgggtgg tcaggacaag ggaataaagc
     3961 aggaattgta tgagataaaa gaggagaccc aggagataag ggaatttctg gaaagctacg
     4021 atgcgcgact ttcacgcata gaaatgaagt taggggagtt attcaaagaa aaagagggct
     4081 ttccatctga tatcgaaaaa cttaatgata tcaaaaccct tctggcttca ttaagggagg
     4141 atgctaatga tgttttaggg catggggaat atgtggataa gaagataaaa gagtttgaga
     4201 acaggataga cgatttggaa aaagataaac cccaaggcta gaggatgata tatgaaaaag
     4261 ataagcattc ttctgttggc tgttagtttg gtattttgtt tctgtggaaa gagcggcttc
     4321 catcctaaag tcgccataat ttatgctttt tcaactgaag gagagttact gaagagccag
     4381 accaaattag tcaaaaaatt cgatataaac ggcagggaat tttatctggg aaagttgaaa
     4441 ggcacaagcg tagttattgt caattcagga gtagggatga ccaatagtgc tatgactacc
     4501 cagctggtca ttgacaggtt ctatccggag aagataatct tcaccggaat atgcggagga
     4561 attgactcgt ccaataaaat cggagatgtc gtcattcctg aaaaatgggt aacacatgat
     4621 ttggggatat ataaaaaggg tggctttttt gctgattcag ttggatattt ctcccatatg
     4681 gaaaacaaag taatatatca gatgtactta ccggttgatt cttcgctttt ggcgattgct
     4741 caaagttctg ccacgaagct gaaaggaagc ctaaaaccga tcggcacgag ggagccgaat
     4801 ataaaaatcg gtggaattgg agctagcggg aattctttta ttgaccagaa agaaaaaaga
     4861 aaatggctaa aagaaaaatt agatgcccaa attgtggata tggaatcagg cgcggtggtg
     4921 caagtggcat tgtccaacaa tattccggta atagttgttc gcagttgttc cgatttggca
     4981 ggcggaagtg gctccaacac agcccaagag gaattgaata agtttttcag agtagcggca
     5041 gataacagtg cgatgatggt gggagagatg gtggaagcat taaagtgatt tttcaggttc
     5101 tgtcaggagc tactcctgac actttcacct gttctatgcc agagtggtat agggtctgcc
     5161 acgtctaaag gatttagcag aaacgaagaa tgaagggaca ggtcgtgacc tgtccgagag
     5221 cggtgtcgtc attcctgtcc cgccttaagc gggataaatt ccaacaggaa tccatttatg
     5281 ttttaattgg aattgtcaaa aactggattc ccgccttcgc gggaatgaca attcaaatag
     5341 aagccactgc accgtttcgg tacgggacga agcatatcta tcgcccgaag aacacgggtg
     5401 gagatacggt tcgaccttgc tcaccgtcct gagcaaagtc gaaggaaact ccacccctat
     5461 gcattgatta ttctacagaa tcaggaagat cttatggatt gggttgttct tttggtagtt
     5521 ggcttagcca tcttttgtgg tttttttgtg caaaccataa ttggttttgc cgcctcggtg
     5581 gtcgctgtgc cgataatttt gtcggtgtgc aaactgcagg atgcagttgc cataatggcg
     5641 attttgctat ttgttttcag ctttatatta gtccttaaga attacaaaga gatagataag
     5701 aaaatgttat tggagatggg gattggaatt atcctgggca taatagttgg agtggtagtc
     5761 ttaagacatg ccaacccaat tatattgcaa aaaggtttgg gagtatttgt tgtgctatat
     5821 gctctttatt ccttaaacaa gcaaaagaca attgaagtct tcaagaaatt gggacttttg
     5881 tttggatttg tgggagggat attctcgggc ttatatacga ccggtggacc tgtctatgtt
     5941 acttacataa ccaatagatt atctaaagcc tctaatttaa gggcaactat aattggagta
     6001 atgggagtaa gcaaccttgc aagattaccc atactttttg ccaacaattt agtccctttt
     6061 cattgcataa agatagcaac ctttgtattc cccttgtttg ttctgtcctt atatttgggt
     6121 catatagtct atccaaaaat aaatgaatgt ctttttaaga gaatcgtatt ggcggttctt
     6181 ttgataagtg gcctactctt aatcgtgaaa tagggggata agagagtctg cacttaaaca
     6241 attcccaaaa ttctcctatc tgttttttga acgatttata gcagaaatca caagacttga
     6301 tataacaata accgacccca accacaagac ccagataaaa gatttcttgc tgatttccaa
     6361 caccagaacg ggttgagtta tctcctcatt tgtttctatg ctcaatgata ttgtcttgaa
     6421 atcagcatct atttcctcta aatagagata atcttcggta ttgaacagct gcacctttct
     6481 taattcatcc tgctgtatag gtcccatcac tatggcagga attgattttt gtttttgctt
     6541 gtccttgaat atttccagaa tggctccaac cttaatgagt tcggcttgag catgcggggt
     6601 tctttcaaat ccaacaaact ttatcttata ttcacctatc tttttggttt cgccttcctt
     6661 taatttgaaa ttctctttct tgggaccttc ctggtattct atgggtgcca gatataaatc
     6721 cccccaaaga ttggatttaa tgtagggttt tttcatcacc ccctgattat actcactgta
     6781 atagaaagct ggccgggcaa tggttttctt gccgtctttc tcaatttcaa tctttagaaa
     6841 acccctctca tttgtgttct caaaacccaa ataggtgaag ttatagccaa gggcagattt
     6901 agtttggttt ggagataaat ttattctgtc tgcgatacta tagccagatg aggtaactat
     6961 tccaattagc atcaatccca tgcccaaatg ccccaagaga ctagtccaag ctatggtttt
     7021 acttcttatc ctttttatca aataaaacaa atttcctgtg aataccaaaa aagcaaaaaa
     7081 gataaggaga gtataagagg ttttaagaca acccagataa gctatggata gtagcgtcaa
     7141 gattagactc aaaaccaaaa ggggaaacat atttttgaat atctgcaaga attctgtttt
     7201 cttccagttg agaaatataa ccaaactcga aagcaaggct ataattagcc ccaaaggcag
     7261 ggttattttg acatagtagc ttgttccaac ttgggatgct tgcccaaaca tcttagtgat
     7321 aaagggagag gatgtaccca atagaatcaa aactgcccag atgaggatta aagccgatcc
     7381 cataataacg aaagactgct tagagagaag cggtttttca ccaatagagt ttttcaaatc
     7441 cctccatcga agtatgacca aaatcaaaga gataaaagag aagaaaaaca taaacacgat
     7501 caagaaaaca ttaattccca aatcggtgaa ggagtggagg gaaaattcag ctaaaactcc
     7561 actgcgggtt aaaaatgttc cgtacatcac gagcagaaaa gagaacaaag ccagaataag
     7621 gttggtgcgt tctgctcccc ctttatattt ttccaaaagc atagtgtgaa gcagaaccac
     7681 gcttaccagc caaggcacca aagaagcatt ttcaaccggg tcccagcccc aatatcctcc
     7741 ccagcccata actttgtaag cccaataacc gccaatgaaa attccagctc ctaagaaaag
     7801 acagctgaag gagacccagg gcaaggcgaa gcttacccaa tccttgtatc tattttgaac
     7861 cagagctgag agggcatagg caaagggtat ggcaaagccg gcatatccca caaagacaat
     7921 cggcggatgg ataaccatcc aaaaatcctg caaaagtgga tttaaccccg caccctccaa
     7981 aggaaagagg ggcagctctt caaaagggct tttcttcaaa agcacagcta agaggaaaat
     8041 ctggattaaa agataaaaga aaagtagatg attcttatag accccttgct ttttaatgag
     8101 gaataaaccc aagatagctc cgaagaaaag ccacaaaaga aaagtcccct cctggcctgc
     8161 ccaaaaggag gagacaagat aaaagatggg cgaatccgaa gagctataat tatagacgta
     8221 agaaaactca aagcggtggg ccaagaaaag ataaaacagg tgagccgtag ccaagagagt
     8281 gaaaccgaag aagaagtagt aactcttctg ccca
//
LOCUS       DASWLN010000018         1665 bp    DNA     linear   ENV 20-FEB-2024
DEFINITION  MAG TPA_asm: Candidatus Zixiibacteriota bacterium isolate
            SMAG_U2949 k141_1151348, whole genome shotgun sequence.
ACCESSION   DASWLN010000018 DASWLN010000000
VERSION     DASWLN010000018.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37960594
            Sequence Read Archive: SRR10376091
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Candidatus Zixiibacteriota bacterium (soil metagenome)
  ORGANISM  Candidatus Zixiibacteriota bacterium
            Bacteria.
REFERENCE   1  (bases 1 to 1665)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 1665)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (26-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/12/2024 08:28:26
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 1,440
            CDSs (total)                      :: 1,416
            Genes (coding)                    :: 1,412
            CDSs (with protein)               :: 1,412
            Genes (RNA)                       :: 24
            rRNAs                             :: 1 (16S)
            partial rRNAs                     :: 1 (16S)
            tRNAs                             :: 21
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 4
            CDSs (without protein)            :: 4
            Pseudo Genes (ambiguous residues) :: 0 of 4
            Pseudo Genes (frameshifted)       :: 0 of 4
            Pseudo Genes (incomplete)         :: 3 of 4
            Pseudo Genes (internal stop)      :: 1 of 4
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..1665
                     /organism="Candidatus Zixiibacteriota bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_1151348"
                     /isolate="SMAG_U2949"
                     /isolation_source="soil"
                     /db_xref="taxon:2053527"
                     /environmental_sample
                     /country="USA: Oregon"
                     /lat_lon="42.99 N 123.63 W"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            <1..214
                     /locus_tag="VGB16_00575"
     CDS             <1..214
                     /locus_tag="VGB16_00575"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=2
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEX9916205.1"
                     /translation="GEVEDREGQRKVLLKTLEVLERAKKPGEVWHLPFTWPEEPKKTD
                     WQPPEMSPLIKFYLEDIKKARRQQEG"
     gene            complement(712..>1665)
                     /locus_tag="VGB16_00580"
     CDS             complement(712..>1665)
                     /locus_tag="VGB16_00580"
                     /EC_number="2.3.1.-"
                     /inference="COORDINATES: protein motif:HMM:TIGR01930.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016746 - acyltransferase activity
                     [Evidence IEA];
                     GO_process: GO:0006635 - fatty acid beta-oxidation
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="thiolase family protein"
                     /protein_id="HEX9916206.1"
                     /translation="DYVSAVQVNRACCSSMEAIRIASMAIRQGEAKAIIAGGSESMSN
                     VSYSIKNARWGMRLQHRELTDGVWDGLTDPYTGLIMGMTAENVAEKYNVSREDQDKFA
                     YRSQMRTKKAIEEGRFKDEIVAVTIPGQKKGELKIVDTDEHPRPETTLDKLGKLSPAF
                     KKDGTVTAGNSSGINDGASAVLVLSRELADKLNMKRKWKVVGSAAVGVDPRIMGIGPI
                     PAIKKLLKQTGYDLKDIELFEINEAFAAASLAVERELGLNPDIVNVNGGGIALGHPIG
                     NTGCRIVVSLIHEMEKRGLKLGLAALCGGSGHGEAILIEKA"
BASE COUNT          475 a          394 c          328 g          468 t
ORIGIN      
        1 gggagaagta gaggaccggg aaggtcaaag gaaagttctg ttgaaaactc ttgaagttct
       61 tgaaagggcc aagaaaccag gagaggtctg gcatctaccg tttacctggc cagaggaacc
      121 aaaaaagaca gattggcaac cccccgagat gtcaccactc atcaagttct atttggaaga
      181 cattaagaaa gccagaaggc agcaggaagg ttaaaagatg tggaagctga gacttgattt
      241 agcagagggt gtcaaatcaa gtcccgatta actatttcag ctacgaatgt tcactctaag
      301 ttagttcata gaggtaacga ttattgagta aaagcgcgca tcatacctct ggagtaattt
      361 aatttctggc cttctttagg acagtttaaa tatttatgac tcgcgcgaac aacttcattg
      421 gtagtttaag ataagaccgt acgatcaaac ttcggaatag caggtggatc aaacaatcag
      481 aagaatagtg gttagtggtg gatagaagaa agccattcag cattgtttag aaaattaggt
      541 ttcctatttc tatctcgaat gtttatcttt tttaccaatt tcaagcattt tcatgggaaa
      601 tagatcaagg agcctagaat ggaaatagcc aatgtgtcat gaataggatc aaggcaaaaa
      661 cttagaactt cagaaacgaa gatatttctt ttcttcatac ctctagtttt tctatgcttt
      721 ttctatcagg attgcctcac catgtccgct accaccacat aaagcggcaa gtccgagttt
      781 taatccacgt ttttccattt catggatcag acttacaacg atacgacaac ctgtattccc
      841 aattggatgg cctagagcaa ttccaccccc attaacgttg acaatatcag gatttaaacc
      901 aagctcgcgc tctacagcca aagaagctgc agcaaaagct tcgttgattt caaaaagctc
      961 gatatctttc agatcatagc ctgtctgctt caaaagtttt ttgattgccg ggatggggcc
     1021 aatacccatg attcggggat ccacgcccac cgccgctgag cccacaactt tccacttcct
     1081 cttcatattc agcttgtctg ctagctctct tgacaagacc aaaacagcgg aggccccatc
     1141 atttattccc gaagagttac ctgctgtaac cgtaccgtct ttcttaaagg caggggatag
     1201 tttgcctaat ttatccaaag tagtctctgg ccttggatgt tcatccgtgt ctactatttt
     1261 aagttcacct ttcttttgac caggtatcgt aaccgccaca atttcatcct taaatcttcc
     1321 ctcttcaatt gctttcttag tccgcatctg acttctatag gcaaacttat cctgatcctc
     1381 tctcgaaaca ttgtattttt cggcaacatt ctccgctgtc atgcccataa tcagccctgt
     1441 gtaaggatct gtcaagccat cccaaacacc atccgtaagc tcccggtgct gcaatcgcat
     1501 tccccatcga gcgttcttaa tcgaatagga tacgttgctc atactctcac tgcctccggc
     1561 aatgatcgcc ttcgcctcac cctggcgaat ggccatggag gcaattctta ttgcttccat
     1621 agaagaacaa caagctcggt tgacctgaac ggcagaaaca taatc
//
LOCUS       DASWLN010000019         4149 bp    DNA     linear   ENV 20-FEB-2024
DEFINITION  MAG TPA_asm: Candidatus Zixiibacteriota bacterium isolate
            SMAG_U2949 k141_1152287, whole genome shotgun sequence.
ACCESSION   DASWLN010000019 DASWLN010000000
VERSION     DASWLN010000019.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37960594
            Sequence Read Archive: SRR10376091
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Candidatus Zixiibacteriota bacterium (soil metagenome)
  ORGANISM  Candidatus Zixiibacteriota bacterium
            Bacteria.
REFERENCE   1  (bases 1 to 4149)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 4149)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (26-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/12/2024 08:28:26
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 1,440
            CDSs (total)                      :: 1,416
            Genes (coding)                    :: 1,412
            CDSs (with protein)               :: 1,412
            Genes (RNA)                       :: 24
            rRNAs                             :: 1 (16S)
            partial rRNAs                     :: 1 (16S)
            tRNAs                             :: 21
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 4
            CDSs (without protein)            :: 4
            Pseudo Genes (ambiguous residues) :: 0 of 4
            Pseudo Genes (frameshifted)       :: 0 of 4
            Pseudo Genes (incomplete)         :: 3 of 4
            Pseudo Genes (internal stop)      :: 1 of 4
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..4149
                     /organism="Candidatus Zixiibacteriota bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_1152287"
                     /isolate="SMAG_U2949"
                     /isolation_source="soil"
                     /db_xref="taxon:2053527"
                     /environmental_sample
                     /country="USA: Oregon"
                     /lat_lon="42.99 N 123.63 W"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            <1..213
                     /locus_tag="VGB16_00585"
     CDS             <1..213
                     /locus_tag="VGB16_00585"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEX9916207.1"
                     /translation="EAAVIPVQSAVGEIIELGISIQQMKFEDKKIVGFQVTVRKDGKE
                     LERWPSMDLVRFEIPTERSDQIFWQI"
     gene            239..613
                     /locus_tag="VGB16_00590"
     CDS             239..613
                     /locus_tag="VGB16_00590"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEX9916208.1"
                     /translation="MVEPFFVDDDENEKKIETETKRETETNEGKNAAIFAYVPFLCFI
                     PLVKMRNNEFALKHGKQGFMLLLIEIISFLFLVVPKLNELLWAALIILCAISAAAGIL
                     HVLEGKSWKVPFIGDLAEKFKL"
     gene            complement(1024..3471)
                     /gene="leuS"
                     /locus_tag="VGB16_00595"
     CDS             complement(1024..3471)
                     /gene="leuS"
                     /locus_tag="VGB16_00595"
                     /EC_number="6.1.1.4"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011994670.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0005737 - cytoplasm [Evidence IEA];
                     GO_function: GO:0004823 - leucine-tRNA ligase activity
                     [Evidence IEA];
                     GO_process: GO:0006429 - leucyl-tRNA aminoacylation
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="leucine--tRNA ligase"
                     /protein_id="HEX9916209.1"
                     /translation="MKTYPSREIEAKWQKIWEENKLYKTPEFPKKKYYMLEMFAYPSG
                     DIHIGHFRNYIIGDAVARQKMMQGYEVLHPFGWDAFGLPAEIAAIERNVHPREWTLSN
                     INVSRSTLKRIGLSYDWDREVITCLPDYYRWTQWMFIQLYKNNLAYRAQSLVNWCPTC
                     KTVLANEQVEQGRCWRCKSEVTKKELEQWFFRITAYADRLLSGLDKLDGWRPNVKIMQ
                     RNWIGKSQGVEIDFEIENSDKKISVFTTRPDTIYGVTFMAISPENQLLKELKIDSKHA
                     KAVEEYIRKSIQKSDIERTALGEKDGVFTGVYAINPLSKEKIQLWVGDYVLATYGTGI
                     VMGVPAHDQRDFEFAKKYKIPLKVVIQPEGANLLVDNMQGAYTEPGIMINSDIFNGTW
                     SSDGIQKVNQYVVDRGLGRLKTNYKLRDWLISRQRYWGAPIPIIYCQKCGILPVLEKD
                     LPVLLPEGEIDFIPKGRSPLADVPKFVNITCPKCKGVAKRDVDTMDTFVCSSWYFLRY
                     GDAHNGKEPFSKEKAKFWLPVDKYIGGIEHACGHLLYFRFFTKFLFDIGWLKVDEPAI
                     ELFNHGMVLDSEGQVMSKSKGNVSSPVDLIDKYGVDVSRVAMYFSAPSEREVLWSEEG
                     ITGAKRFLNRVYALLERMKTNGVEFDCNSISDKEKEIYKHLHRTIKKVSEDIDRLQFN
                     TAIASMMEFLNQFETLSGDAKIYGYCLTVLAQLLAPFTPHLSEEIWSGLGKNISIFKC
                     KWPEYNKEAVKFEQITIPVQVNGKLRSTLVVPVDVGEEEVKIKAVQDEKVKPYLEGKR
                     ILKTIFVKNRLINLVVK"
     gene            complement(3783..>4149)
                     /locus_tag="VGB16_00600"
     CDS             complement(3783..>4149)
                     /locus_tag="VGB16_00600"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_004101733.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0000166 - nucleotide binding [Evidence
                     IEA];
                     GO_function: GO:0004812 - aminoacyl-tRNA ligase activity
                     [Evidence IEA];
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_process: GO:0043039 - tRNA aminoacylation [Evidence
                     IEA]"
                     /codon_start=2
                     /transl_table=11
                     /product="amino acid--tRNA ligase-related protein"
                     /protein_id="HEX9916210.1"
                     /translation="KEFDKPVFVYNYPKKARAFYMKPHPQRDDLVLCADLLAPEGYGE
                     IIGGSQRNDDYDSLLKRIKEEKLPEEAYEWYLDLRKYGSVPHSGFGLGVERVVTWICG
                     LPHIRESIPFPRMLYKLYP"
BASE COUNT         1133 a          975 c          777 g         1264 t
ORIGIN      
        1 gaagccgctg ttatccctgt gcaatcggca gttggagaga taattgaatt gggtatttca
       61 atacagcaga tgaaattcga ggacaaaaag atcgtcgggt ttcaggttac agtgagaaag
      121 gacggaaaag agcttgaaag gtggccaagc atggatttag tcaggtttga gattcccact
      181 gagagaagtg accagatatt ctggcagata tagaggactc taaattggag gtggatctat
      241 ggtagaacct ttctttgtgg acgatgacga gaatgaaaag aaaatagaga cagagactaa
      301 acgggaaaca gaaacgaatg aaggcaaaaa tgcagctatt tttgcttatg tgccgttcct
      361 ttgttttata cccttggtta agatgaggaa taatgaattt gccctgaagc acggcaagca
      421 gggttttatg ctcctcttga tcgaaataat atcctttttg ttcctcgtcg ttcctaagct
      481 aaatgaactt ctctgggcgg cactaattat actctgcgct atttctgcgg cagctggtat
      541 tcttcacgtt ctggaaggga agagttggaa ggtcccattc ataggggact tggcggagaa
      601 attcaagtta taataataag atggacgccc aattcaaata aatctggagt tcatcctccg
      661 tgacgatgca ggattgagaa agtgaatata ttgcatagaa gaagtaacgt cagataatat
      721 ctattatgta aactacaact cgtaccaccg accctctata cataatcaac tccttctatt
      781 tcagtcttaa ccatcttcat ttcacccggc ctttatctgc cttttgataa ctactaaggt
      841 ttcaataccc atgactttca gcgactcttc gttcgacgtc cagatgctga aacgagttca
      901 gcatgacaac agggtttggt tttacggttt ttgtcatccc gaacttgatt cgggatcccg
      961 gtttggtttt gaattttagg ttttaaagcc ccgccctgtg ggtggaattc ttcactccaa
     1021 attctacttt accaccaaat ttatcaacct attcttcaca aaaatcgtct ttaaaattct
     1081 ctttccctcg agatacggct tcactttttc atcctgcact gcctttatct taacttcctc
     1141 ctcgcccaca tcaacaggca caacaagagt acttctcagt ttaccattca cttgaaccgg
     1201 aatcgtgatt tgctcgaact ttacggcttc cttattgtat tcaggccatt tacatttaaa
     1261 aatacttata ttcttgccta atccgctcca tatttcttct gaaagatgtg gtgtgaatgg
     1321 cgctaataac tgagcaagta cggttaagca ataaccatag attttcgcgt caccactcaa
     1381 agtctcaaac tgattcaaaa attccatcat acttgcaatt gctgtgttaa actgaagcct
     1441 atcaatatct tcagatactt tcttaatagt tctatgtaaa tgcttatata tttctttctc
     1501 tttatcggat atagagttac agtcaaactc aaccccgtta gtcttcattc tctccaatag
     1561 ggcataaacc ctgtttaaga acctttttgc cccagttatc ccttcctctg accacaatac
     1621 ctctctttca gaaggtgcgg agaaatacat agccactctc gagacgtcaa ccccatactt
     1681 gtctatcaaa tccacaggag aacttacatt tcctttagac ttcgacataa cctgtccttc
     1741 actgtccaaa accattccgt gattaaaaag ctcgattgcc ggctcatcca ctttcagcca
     1801 acctatatca aataaaaact tagtgaaaaa cctaaaataa agtagatgcc cacaagcatg
     1861 ctcaatcccg cctatatact tgtcaaccgg aagccagaac tttgcctttt cttttgaaaa
     1921 tggttcctta ccattgtgcg catccccata tcttaagaaa taccaggacg agcagacaaa
     1981 agtgtccatg gtgtcaacat ctcttttagc cacccctttg catttggggc aagtaatatt
     2041 gacaaatttc ggcacatcag ccaaagggga cctgccctta ggtatgaagt cgatttcccc
     2101 ctctggcaaa agcactggta gatctttctc caaaacaggc aaaataccgc acttctggca
     2161 ataaattata gggatagggg caccccaata cctttgccgt gagataagcc agtctcggag
     2221 cttataattc gttttaagcc tacccaggcc cctatcaacg acatattgat taactttttg
     2281 gattccatcg cttgaccaag tgccgttgaa tatatctgaa ttaatcataa ttccaggctc
     2341 agtatatgct ccctgcatat tgtctaccaa taagttagct ccttctggtt gaattaccac
     2401 tttaagtggg attttgtatt ttttggcaaa ttcaaagtct ctctggtcat gggctggtac
     2461 acccatcact atccctgtgc cataagtggc taaaacataa tcacctaccc aaagctggat
     2521 tttctctttt gagagtgggt ttatagcata gactccggta aagactccat ctttttctcc
     2581 taaagcagtt ctttcgatat ctgacttctg aatggacttt cggatgtatt cctctactgc
     2641 ttttgcgtgt ttagaatcta tctttagttc cttgagaagt tgattctccg gggagattgc
     2701 cataaaagta acaccataga tagtatcagg tcgagtggta aagacggata tcttcttgtc
     2761 gctattttcg atttcaaaat ctatttccac tccctggctc ttccctatcc agttcctctg
     2821 cataatcttt acgttcgggc gccatccgtc taacttatct aagccactta gtaacctgtc
     2881 ggcatatgct gtgattctga aaaaccactg ctccaattct ttttttgtca cttcgctttt
     2941 acatctccag catctcccct gctcaacttg ttcatttgcc aaaaccgtct tgcaggttgg
     3001 acaccagttg accaaggatt gtgccctgta tgctagattg tttttgtata gctgaatgaa
     3061 catccactga gtccatctgt aatagtctgg caggcaggtg atgacctccc tgtcccaatc
     3121 ataacttaga ccaattctct ttagggtcga cctagagaca ttgatattgc tcaaagtcca
     3181 ctcacgagga tggacatttc tctctatggc ggctatctca gccggcaagc cgaaagcatc
     3241 ccaaccgaaa ggatgcagga cttcatatcc ttgcatcatc ttctgtcggg ccactgcatc
     3301 cccgattatg tagttcctga aatgtccgat atgaatatct ccggaaggat aggcgaacat
     3361 ctctaacata tagtatttct tcttgggaaa ctcaggagtt ttataaagct tattctcctc
     3421 ccagattttc tgccatttgg cttctatttc tctagatggg taggtcttca aatttaactc
     3481 ctcaaaaata gagctttgtg tattttttct gtagggacag aacagtgttc tgtccctcat
     3541 tcttgcgtag attcgctcga cccttcgaca agctcagggt ggtgagcaac gtcgaaccat
     3601 aaactcatgg tgctgagcga agtctaagca ctggctcaag acggtgagca aagtcgaact
     3661 gtgaatcgag caactaagct ttgaatttgg cagggctaaa tgccctgccc tacgaaaatc
     3721 cactctcctt gtagggacgg gaatttattc catccctcgt agatgaatcg agcaagaaca
     3781 ctttacgggt acaatttata caacattctc ggaaaaggta tactctccct tatgtgcggc
     3841 agaccgcaaa tccatgtaac tactcgttct acacccaaac cgaatccaga atgaggaacg
     3901 gagccgtatt tcctcaaatc cagataccat tcgtatgcct cttccggcag tttctcctct
     3961 tttatccttt taagtaaact atcgtaatca tcatttctct gtgacccgcc aattatctct
     4021 ccataccctt ctggagccaa aagatcggca cataaaacta aatcgtctct ctggggatgg
     4081 ggcttcatat agaaagcgcg cgctttcttg ggatagttat atacaaagac tggtttgtca
     4141 aattcttta
//
LOCUS       DASWLN010000020         2914 bp    DNA     linear   ENV 20-FEB-2024
DEFINITION  MAG TPA_asm: Candidatus Zixiibacteriota bacterium isolate
            SMAG_U2949 k141_654066, whole genome shotgun sequence.
ACCESSION   DASWLN010000020 DASWLN010000000
VERSION     DASWLN010000020.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37960594
            Sequence Read Archive: SRR10376091
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Candidatus Zixiibacteriota bacterium (soil metagenome)
  ORGANISM  Candidatus Zixiibacteriota bacterium
            Bacteria.
REFERENCE   1  (bases 1 to 2914)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 2914)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (26-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/12/2024 08:28:26
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 1,440
            CDSs (total)                      :: 1,416
            Genes (coding)                    :: 1,412
            CDSs (with protein)               :: 1,412
            Genes (RNA)                       :: 24
            rRNAs                             :: 1 (16S)
            partial rRNAs                     :: 1 (16S)
            tRNAs                             :: 21
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 4
            CDSs (without protein)            :: 4
            Pseudo Genes (ambiguous residues) :: 0 of 4
            Pseudo Genes (frameshifted)       :: 0 of 4
            Pseudo Genes (incomplete)         :: 3 of 4
            Pseudo Genes (internal stop)      :: 1 of 4
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2914
                     /organism="Candidatus Zixiibacteriota bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_654066"
                     /isolate="SMAG_U2949"
                     /isolation_source="soil"
                     /db_xref="taxon:2053527"
                     /environmental_sample
                     /country="USA: Oregon"
                     /lat_lon="42.99 N 123.63 W"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            <1..643
                     /locus_tag="VGB16_00605"
     CDS             <1..643
                     /locus_tag="VGB16_00605"
                     /inference="COORDINATES: protein motif:HMM:NF015013.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=2
                     /transl_table=11
                     /product="lipid II flippase MurJ"
                     /protein_id="HEX9916211.1"
                     /translation="VDTCTSSVKLVFFLTLPSAFFLGVASKPIISLLYQHGQFSFLDT
                     VNTSGALIFYSIGLFGYASSRVLVPAFYSVGNAKTPVTIGIFAVALNVGLNLILMGPL
                     GFRGLAFGTSIAAIFNMFFLIYAMNKIISPINTKDLVVSFVKILFASAFMGMCLTLFL
                     TASSCNLQVAGILQKIILVLLAGIVCLGSYFLACYLLKIKELERFLEMIRLRR"
     gene            734..>2914
                     /locus_tag="VGB16_00610"
     CDS             734..>2914
                     /locus_tag="VGB16_00610"
                     /inference="COORDINATES: protein motif:HMM:NF013506.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008233 - peptidase activity [Evidence
                     IEA];
                     GO_process: GO:0006508 - proteolysis [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="S49 family peptidase"
                     /protein_id="HEX9916212.1"
                     /translation="MKRVCLLLVFCVVLVRMGLADAQEGPISSYYTQVDFLSTSPSSE
                     AGAIGAYVNPATVGMLPGFELEYFWSDQDAKFKSIKRWGLFSGIPHLGFGVVHQKLPS
                     SQSVTDYRLGVAFGDRGNSFGLGYGWSTGDVTQRPGDIFIVGSCFRPCRVASLGLVGN
                     FATKHSHRSGVVDLGIRPLGNSLITLFADAEMHKQDKLEDARWGVGLALEPIRGFQVV
                     GKYLDNKFFSLGLNFSLGKKVFSSVAHLDDKQKVSYTTYGLRTGYPIDNIFDRYLKKN
                     KSYLSLNLKGNVAYQKYRFFDDETHTLSGILFSLEDAIQDPRVAGVALNLSGIEFSGE
                     FAWEIHQKLIEVKEAGKKVVIFIDGAGMPEYHLASVADRIVMDPMGLIALPGYVAGRT
                     YYRNMLEKMGVGFDEWRFFTYKSANESFSREKMSEPDREQRQELVDDFYALVRKDVAS
                     SRNISEERFDFWVNNKMIFLAKDALDSGLVDTLARWKDIDEVIKSLEGKNKGTIEAKR
                     FVDEKFPSRTWGEKPKIVVVYALGVCAMDEGINARQLEGIFRQLEGDSRVKAVVFRVD
                     SPGGDALASDVVAEALRKCAKKKPVIVSQGGVAASGGYWISMYGDTIVAAPNTVTGSI
                     GVIGGMIYNQGIGSKLGLTSDYVKAGEHADFGFGITIPILGITVPDRKPTPEEGKCIE
                     ELIKMGYKEFVSKVANGRKLSEEKVDSIGQGRVWSGIDGKEKGLV"
BASE COUNT          733 a          527 c          806 g          848 t
ORIGIN      
        1 ggttgacact tgcacttcct cagtcaagtt ggttttcttt ttgactttgc catccgcatt
       61 tttcttaggc gtagcatcca agccgattat atctctttta tatcagcacg gacaattttc
      121 atttttggat acagtcaata cttcaggtgc tttgatattc tactccattg ggcttttcgg
      181 ttatgcttcc tccagagttt tggtgcctgc attttattcg gttggaaatg ccaaaacccc
      241 ggtcaccatc ggcattttcg cggtggcttt gaatgtcggt ttgaatttga ttttgatggg
      301 accgctcgga tttagggggc ttgcttttgg aacttccatt gcggcgatat tcaatatgtt
      361 tttcttgatt tacgctatga acaagataat ttcgccaatt aacacaaagg atttggtagt
      421 ctcttttgtg aaaattctat ttgcttccgc ttttatggga atgtgcctga ctttgttttt
      481 gaccgcttcc tcctgcaatc tgcaggttgc cggcatcctg caaaagataa tcctcgtcct
      541 cttggctggg attgtttgtt tgggaagtta tttccttgcc tgctatcttt taaagattaa
      601 agagctggag agatttttgg agatgataag attaaggaga taataaagtt gacttttgct
      661 ggttgaaaca ttgtttttat ttgtccgtag atgaaaataa aggagttcta acctcttgga
      721 taaaggaggg cagatgaaaa gggtttgtct tttgttagtg ttttgcgtgg ttttggtgcg
      781 gatggggtta gcagatgcgc aagagggacc tatttcttcc tattatactc aagtagattt
      841 cctttcaact tccccttcca gcgaagcggg tgcaattgga gcttacgtca atccagccac
      901 agtggggatg ttgccggggt tcgagttaga gtatttctgg tcggaccaag acgccaagtt
      961 taaatctatc aagagatggg gtttgttcag cggtattccg catttgggat ttggagtggt
     1021 tcatcagaag cttccttcat cccagagcgt aaccgattac cgtttgggag ttgcgtttgg
     1081 agatagagga aatagctttg gtttgggtta tggctggtcg acaggagatg tgacccaaag
     1141 gccgggtgat attttcatag ttggctcatg ctttaggcct tgcagggttg cctccttggg
     1201 tcttgttgga aatttcgcca ccaaacattc acacaggagc ggagttgtag atttgggaat
     1261 ccgtcccttg ggaaattcac ttattacttt gtttgcggat gcagagatgc acaaacagga
     1321 taaattggaa gacgctcgct ggggagtggg gttggctttg gagccaatcc gtggctttca
     1381 ggtggtggga aaatatcttg ataataaatt cttcagcttg ggattgaact tcagcctggg
     1441 caaaaaggtt ttctcctcag ttgcccattt agacgacaaa cagaaagtaa gctacaccac
     1501 ctatgggctt cgtacgggat atccaataga taacatcttt gatagatatc tcaagaagaa
     1561 caaaagctac ttatctctga acctaaaagg gaatgtcgcc tatcagaagt atcgcttctt
     1621 cgatgatgaa actcatacct taagtgggat tttattctct ctggaggatg ctattcaaga
     1681 cccgagagtt gctggagtcg ccttgaacct ttcgggtata gagttctccg gcgagttcgc
     1741 ctgggagatt caccagaagt tgatagaggt aaaagaagcc ggcaagaaag tagtcatttt
     1801 catcgatggg gcggggatgc cagaatacca cttagccagc gtggcggaca gaatcgtaat
     1861 ggaccccatg ggactgatcg cccttccggg atacgtagcc ggacgcacct actatcgcaa
     1921 tatgctggag aaaatgggtg ttggttttga cgaatggcgg tttttcactt acaagtcagc
     1981 caacgagagt ttttcccgag agaagatgtc agagccggat agggagcaaa ggcaagagct
     2041 ggtggatgac ttctatgctc tggtgaggaa ggatgttgct tcctcacgca atatctcaga
     2101 ggagaggttc gacttttggg tcaacaataa gatgatcttt ttggcaaagg atgctttaga
     2161 cagcgggttg gtagatacgt tggcaaggtg gaaggatatt gatgaggtca tcaaatcatt
     2221 agaagggaaa aacaaaggga cgatagaagc taaaagattt gtagatgaga agttcccttc
     2281 gcgcacctgg ggagaaaagc caaagatagt agttgtctat gctttgggtg tgtgtgctat
     2341 ggatgagggg attaacgcac gccaactgga ggggatattt aggcaacttg aaggtgatag
     2401 tcgagtgaaa gcggtggtct tccgagttga ctctccgggt ggagacgcct tagcttcaga
     2461 tgtggtggct gaagccttaa ggaagtgtgc taagaaaaag ccggtgattg tgagccaagg
     2521 gggagtagct gcttctgggg gttattggat ttcgatgtat ggggatacca tcgtagctgc
     2581 gcccaacact gtaaccggtt ccatcggagt aatcggagga atgatctata accagggaat
     2641 aggttcgaaa ttgggactta cttcggatta tgtgaaagca ggtgaacatg cggattttgg
     2701 ctttggaatc actattccga ttttgggtat aactgtgcca gaccgcaagc ccactccgga
     2761 agagggaaag tgcatagagg agctgatcaa aatgggatac aaggagttcg tctccaaggt
     2821 ggccaatgga aggaagctga gcgaggaaaa ggtggattcg attgggcagg gaagggtttg
     2881 gagtggtatt gatggcaagg aaaaaggttt ggtc
//
LOCUS       DASWLN010000021        15547 bp    DNA     linear   ENV 20-FEB-2024
DEFINITION  MAG TPA_asm: Candidatus Zixiibacteriota bacterium isolate
            SMAG_U2949 k141_770919, whole genome shotgun sequence.
ACCESSION   DASWLN010000021 DASWLN010000000
VERSION     DASWLN010000021.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37960594
            Sequence Read Archive: SRR10376091
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Candidatus Zixiibacteriota bacterium (soil metagenome)
  ORGANISM  Candidatus Zixiibacteriota bacterium
            Bacteria.
REFERENCE   1  (bases 1 to 15547)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 15547)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (26-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/12/2024 08:28:26
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 1,440
            CDSs (total)                      :: 1,416
            Genes (coding)                    :: 1,412
            CDSs (with protein)               :: 1,412
            Genes (RNA)                       :: 24
            rRNAs                             :: 1 (16S)
            partial rRNAs                     :: 1 (16S)
            tRNAs                             :: 21
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 4
            CDSs (without protein)            :: 4
            Pseudo Genes (ambiguous residues) :: 0 of 4
            Pseudo Genes (frameshifted)       :: 0 of 4
            Pseudo Genes (incomplete)         :: 3 of 4
            Pseudo Genes (internal stop)      :: 1 of 4
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..15547
                     /organism="Candidatus Zixiibacteriota bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_770919"
                     /isolate="SMAG_U2949"
                     /isolation_source="soil"
                     /db_xref="taxon:2053527"
                     /environmental_sample
                     /country="USA: Oregon"
                     /lat_lon="42.99 N 123.63 W"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            complement(<1..525)
                     /locus_tag="VGB16_00615"
     CDS             complement(<1..525)
                     /locus_tag="VGB16_00615"
                     /inference="COORDINATES: protein motif:HMM:NF018798.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF1385 domain-containing protein"
                     /protein_id="HEX9916213.1"
                     /translation="MSKPKVGGQAVIEGVMIRSPWRISTAIRKPDGGIEVRGQDYVAF
                     SKRYKILNIPILRGVINFFEMLVIGISTLNFSANFALDEAEKLEGKKPSSKRSKDMAL
                     ALTVIIAFALGMLVFFFFPLFISNFVGISHDAFAFNLLTGLIRVTLFLGYLWGISLFG
                     EFKKIFQYHGAEHKT"
     gene            complement(593..814)
                     /gene="rpmE"
                     /locus_tag="VGB16_00620"
     CDS             complement(593..814)
                     /gene="rpmE"
                     /locus_tag="VGB16_00620"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_013759223.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0005840 - ribosome [Evidence IEA];
                     GO_function: GO:0003735 - structural constituent of
                     ribosome [Evidence IEA];
                     GO_process: GO:0006412 - translation [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="50S ribosomal protein L31"
                     /protein_id="HEX9916214.1"
                     /translation="MKEKIHPQYFETTITCACGNVINTRSTVKDIHVEICSACHPFFT
                     GKQKLIDSAGRVERFIKKYGKKEENKKEQ"
     gene            1328..1834
                     /locus_tag="VGB16_00625"
     CDS             1328..1834
                     /locus_tag="VGB16_00625"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEX9916215.1"
                     /translation="MEEKFYYCKWFIGLAIFEFIISLGIGFMIIRDALDNLFIWHSGD
                     RLGWIVAAISGLLLIVVGLAFLRCVAKPAIIMTQMKIAFFPLSIKANWKPYFFNLRKL
                     ELGVEQIVSARITEMPSVYPFEKPYKVVEVKFRRPSGVEDSFYIFTSKIAKKDRLIDI
                     LKQHIKFE"
     gene            complement(2027..3853)
                     /gene="glmS"
                     /locus_tag="VGB16_00630"
     CDS             complement(2027..3853)
                     /gene="glmS"
                     /locus_tag="VGB16_00630"
                     /EC_number="2.6.1.16"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_018197769.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004360 - glutamine-fructose-6-phosphate
                     transaminase (isomerizing) activity [Evidence IEA];
                     GO_process: GO:0006040 - amino sugar metabolic process
                     [Evidence IEA];
                     GO_process: GO:0009252 - peptidoglycan biosynthetic
                     process [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="glutamine--fructose-6-phosphate transaminase
                     (isomerizing)"
                     /protein_id="HEX9916216.1"
                     /translation="MCGIVGYVGTKKAVPILLEGLKRLEYRGYDSAGIAILEEGGLLS
                     KKTAGKIVALETLLKDDNFKGTVGIAHTRWATHGEPTDLNAHPHVDCSGKIAVVHNGV
                     IENFHSLKELLLRKGHKFISDTDTEVIAHLIEEYYEGSLVEATRSALSLVEGTYGIAV
                     ISKDNPDEIVAARHGSPLVLGSGKDENFVASDVAAILRHTNQVVYLQDKELASITKDG
                     FSISTIDNVKVTPHIQEISWSLDMIEKEGFPHFMLKEIFEQPTTLRNAMRGRLNYEEG
                     TARLNGLNLQYEQLHLIDRIILTACGTAWHAALVGEYVIEKYARIPVEVEYASEFRYR
                     SPIIRDGTTVFVISQSGETADTLAALKEAKRKGATVLGICNVVGSTIARETDGGVYTH
                     AGPEIGVASTKAFTAQMAVLSLLSLLLGRMRDMSLEQGREIINAMQKLPEQVEWMLKN
                     EGQVKEIAKHYYKKNNFLFLGRGVNFPVGLEGALKMKEISYIHAEGYPAAEMKHGPIA
                     LIDENMPVVVVALKDSVYAKVSSNIEEIKARKGSIIAIATEGDTQIAKKVDHVIYIPQ
                     TLEHLTPLLSIIPLQLLAYHVAVLRGCNVDQPRNLAKSVTVE"
     gene            complement(3872..5215)
                     /gene="glmM"
                     /locus_tag="VGB16_00635"
     CDS             complement(3872..5215)
                     /gene="glmM"
                     /locus_tag="VGB16_00635"
                     /EC_number="5.4.2.10"
                     /inference="COORDINATES: protein motif:HMM:TIGR03990.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008966 - phosphoglucosamine mutase
                     activity [Evidence IEA];
                     GO_process: GO:0009103 - lipopolysaccharide biosynthetic
                     process [Evidence IEA];
                     GO_process: GO:0009252 - peptidoglycan biosynthetic
                     process [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="phosphoglucosamine mutase"
                     /protein_id="HEX9916217.1"
                     /translation="MKDKLIVGISGLRGVVGKTLFPETALGYALAFGILCHKGKVVVA
                     RDSRVSGKVLKRAAVSGLLSVGCDVVDLGICPTPTLTLATEKLGAVGGICITASHNPF
                     QWNGMKFVEKDGTFLNKKKMEKLNLLVQKTNISHIKKVPLCKLSPKEDWYKKHIDYIL
                     KLGFVQKDKIRKANLKVVVDCNNGAGSRIAPLLLRKLGCKIVELNCDFSKSFAHAPEP
                     TVENLLSLAKVVRDKNADVGFCLDPDGDRLSLVADEGTPLSEEYTLALATRFILSKNP
                     KEVVINLSTSRMTQEVAEKSGCKVYRTPVGEYNVAKKLKEIKGVIGGEGNGGVILPEL
                     HYTRDALVGMALIVEYLAESNRKLSQIIQDMPKYFMLKKKIEVSKDFDDRLEGLIKRF
                     KKERINKKDGIRIDFKDSWLHIRKSNTEPAARIIAEAKTKKTAEDLAKEGLELLT"
     gene            complement(5309..6181)
                     /locus_tag="VGB16_00640"
     CDS             complement(5309..6181)
                     /locus_tag="VGB16_00640"
                     /inference="COORDINATES: protein motif:HMM:NF015132.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0016020 - membrane [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="EamA family transporter"
                     /protein_id="HEX9916218.1"
                     /translation="MLIYPLFAIIFWGLSFVATKIALRELSPAILILLRFVIGVLILA
                     GALAFKKYPLKIKQSDIFLLLFCGLIIAIHISVQVSGMRFTTASNTAWIVATSPVFVV
                     ILSRLVFKERFSFLKIVGITLAILGVIFLVSKGEPKNISFIRSPGDWMILVSSVTWAI
                     YSVASKKLLDTYSATLITFYSLAIICILTLPLSLNHQSFVSVLELSKVGWMAIAYLGI
                     FCSGIAYLFWSKALKGGNAGEVSVYLYLEPLVATIGASLILREAITFWLLIGGALVIS
                     GIYMVSAKRGLMGG"
     gene            complement(6419..7036)
                     /locus_tag="VGB16_00645"
     CDS             complement(6419..7036)
                     /locus_tag="VGB16_00645"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEX9916219.1"
                     /translation="MIKVVRVFFILVIIFVALLVLKGIMDNVGYLREKNRINYFLDNQ
                     FSKLKFVDLPRLLLTNSKDDLLVSNGIYISDRQKSTEGIKDWRRIQHWIKKNKDFSYL
                     EVDLISFYSPKDVWIFYKSEEDSKILELPHEIFKQKEEGSFGYYISESKPKKINLEEL
                     SPDYPGYASFLLIVKDNLFIEMDESSSSENTDHKLAVLKQITPSQ"
     gene            complement(7033..7443)
                     /locus_tag="VGB16_00650"
     CDS             complement(7033..7443)
                     /locus_tag="VGB16_00650"
                     /inference="COORDINATES: protein motif:HMM:NF025910.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="tetratricopeptide repeat protein"
                     /protein_id="HEX9916220.1"
                     /translation="MKKYAIFSFLIFSFLIFSCSRQTAEDHFKKGVRYQLMANSDSAV
                     LEYQKAIKSDSTYVQAHLNLGVLYTEKGMYDQAIESYKKVIRFTPLHAKAYYNLGYVY
                     TIKGDKEKAQEQYEQLKSLDPQLAQKLKEKIEQI"
     gene            complement(7458..8027)
                     /locus_tag="VGB16_00655"
     CDS             complement(7458..8027)
                     /locus_tag="VGB16_00655"
                     /inference="COORDINATES: protein motif:HMM:NF013077.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="nitroreductase family protein"
                     /protein_id="HEX9916221.1"
                     /translation="MNSQDLLNLIKYRRSIRKFSDKPIEEDKLNLILESARLAPSSSN
                     SQPWHFVVIKDKEVMKKLVGAVPLGIRPNIWFSNVPIVIACLAKPHAIEHQLAKIVAS
                     DYHRIDCSIAIEHMVLMATSLGIGSCWVGWFDQKKTKKILGVPKGWDVVMFLPLGYPA
                     DESNQDGMAGIKPRPRKNLSEMISFNRFH"
     gene            complement(8435..8801)
                     /gene="ssrA"
                     /locus_tag="VGB16_00660"
     tmRNA           complement(8435..8801)
                     /gene="ssrA"
                     /locus_tag="VGB16_00660"
                     /product="transfer-messenger RNA"
                     /inference="COORDINATES: nucleotide
                     motif:Rfam:14.4:RF00023"
                     /inference="COORDINATES: profile:INFERNAL:1.1.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: cmsearch."
                     /db_xref="RFAM:RF00023"
     gene            complement(9383..12154)
                     /locus_tag="VGB16_00665"
     CDS             complement(9383..12154)
                     /locus_tag="VGB16_00665"
                     /inference="COORDINATES: protein motif:HMM:NF013524.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008234 - cysteine-type peptidase activity
                     [Evidence IEA];
                     GO_process: GO:0006508 - proteolysis [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="C25 family cysteine peptidase"
                     /protein_id="HEX9916222.1"
                     /translation="MTKTRLVSGLFAALSLVLFASPLLSQASYNTKYVAEFVQSDLSF
                     DKLAGYDRVRLKGGDWLSDIAKPMLPVKELRIALPAGMKVTDVYATDIKTQEIIGEYN
                     IFPAQPPKKISSSDEEIITVQPDQALYASSQPYPSKLVEFLRQTDLAGQSIAIIRVYP
                     LQYIPAEKKLTLYASLNLVIEGTGGYEYGDYLPPNISDQARETYEQMVKDIVINPEDV
                     HLNTGMAIGAPTLLPPGSFGHAIIAHTYRVSAFQPLVRWHTKKGVKDTVVTTDDIYAA
                     YSGATNQEKIRNFIKDAHTTWGTTYFLLGGENEEVPFEYRTYYNGESTPSDQYYSDYD
                     DDWTNEVFVGRIPAGSSTAVTTFVNKVLKYETDPPRTDYPLDVLLVGMDLDASTPSQD
                     TKEAVYIPSRFNVTKVYDSDGTNHKTATIAALNAGQNLVNHSDHSNYDVMGTGYVNHN
                     LLLSQADVNNLTNDNQMSIIVSTGCLPNKMDAGDCIAETFVEYNPNQAGVAFTGNTRD
                     GWYTQGDPYTLSAALDMQWWAALFNRNKYNLGQALADSKHNFSTPGNIEKHCEWTFNL
                     LGEPEMPIWTDEPDSFAVSFSHAIPIGSSSFLVHVEDSTTHSAVYQAYVCLWKGSEVY
                     LKGNTNTSGNITFNPSPSTQGIMYVTVTKHDYLPCQDSASVASPVVRTDPATEVEETG
                     ATIHGYLDNDGGFEATCWLFWDTDSVVPYANSESLGVFPNASEFSKDLTGLTEGTAYY
                     YIAKGFNIAGWGSGGELAFLTKPLAPTELTAQGVAWNKTSLSWTKPSSADRTVIERDT
                     LTSWTRGEGTEVYNDAGTTCQDSTLVPLTHYYYQAWSYSSEGELSQYSDEFATADATT
                     LFRHGDANADKQITVSDVIYLINYLFKGGPAPVPVMEAGDPNCDAKVTVSDVIYLINF
                     LFKGGPSPC"
     gene            12347..12499
                     /locus_tag="VGB16_00670"
     CDS             12347..12499
                     /locus_tag="VGB16_00670"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEX9916223.1"
                     /translation="MIRIIEMQELDGYQFLNTTTHKQLDKEFRVYLILGKARRKAWLV
                     GIKINY"
     gene            12523..14064
                     /locus_tag="VGB16_00675"
     CDS             12523..14064
                     /locus_tag="VGB16_00675"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_008149602.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="metallopeptidase TldD-related protein"
                     /protein_id="HEX9916224.1"
                     /translation="MPKLTRREFLEMGAKGVVIATIPIIFKPNPAIAFLTPPEGKGKL
                     NDYYKHFGVDEKTIREIMATALERGGDYCDLFFQHRITNSVGLEDNAVDRAYTNVDYG
                     VGIRVIEGEQTGYSFTEETTSKAMKLAARTAANIASQNKTAPPGGLTFHQTPNYYPIL
                     TQWEDVGVDQKIPYLQTINQKVFALDNRVIKSQVSFSNESSYILVATSDGKIACDYQP
                     MTAISVTCSAEQNGRREQNGFNLSGRQGIEFFTPENLDRLAKESVQRTIVMFDAIKPE
                     GGEMEVVLAPGRSGILLHEAIGHGMEADFNRKGISIFSDKINKPVAKEFVSIVDDGTI
                     PNSRGSINMDDEGNDTEKTYLVENGILRSYLHDHISARHYKVKPTGNGRRESFRFPPV
                     PRMRNTYMLPGPHKREEIIGSVKKGLYAESFTNGEVHIGAGDFTFYVKSGWLIEDGKL
                     TRPVKDINIIGNGPKVLENIVMVADDLKLSEGGGTCGKDGQSAPVSMGLPTVKVSSIT
                     VGGVS"
     gene            14102..15424
                     /locus_tag="VGB16_00680"
     CDS             14102..15424
                     /locus_tag="VGB16_00680"
                     /inference="COORDINATES: protein motif:HMM:NF039907.3"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008270 - zinc ion binding [Evidence IEA];
                     GO_process: GO:0006508 - proteolysis [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="TldD/PmbA family protein"
                     /protein_id="HEX9916225.1"
                     /translation="MNKKERLDLANWAVNQAKKSGADQAAIDVSYNRNVEVGFREGKI
                     EQLKESDQNSLSLSVYANRRYSSHRTNDMRKDSLSKFVQEAVAMTKYLNEDPFRLLPD
                     PKYYEGGKKTNLELNDPAYEKVESTKRVEIAQKIEGAARAQSDKIISCTSGYSDNYYE
                     SAKVNSNGFEGEEYGTFFSAGAEVTVNDQRGRRPEDWEWATARFYKDLPSPEGLGKKA
                     SQRALAKIGQSKLESGSYDMILENRVGGRFLYSFFGPMSGRSLQQKSSFLDGKLGQRI
                     GSDKLTLIDDPFIVSGMGSRLFDDEGMATKRRVMIDKGILKAFYIDSYYGRKLGMDPT
                     SGSASNVVFDYGTESLDKLIEGTKRGILVNTFIGGNSNSTTGDFSYGIIGMYIQDGKV
                     IKPVNEMNVSGNLAELLNQLVEVGNDPYIYSSLRTPTLCFKDVQFSGI"
BASE COUNT         4348 a         3515 c         3406 g         4278 t
ORIGIN      
        1 ggtcttatgc tccgccccat gatattgaaa gattttcttg aattctccaa ataaagatat
       61 gccccaaagg tatcccaaaa acagggtcac tcgtatcagc ccggttaaga gattgaaggc
      121 gaaggcatca tggcttatgc caacaaaatt ggagatgaat aagggaaaaa agaaaaagac
      181 cagcatcccc aaagcaaagg cgataatgac agtcaaagcc aatgccatat ccttggaacg
      241 tttagagctg ggcttttttc cctcaagctt ttccgcctca tccaaagcga aattagccga
      301 gaaattaaga gttgagattc cgattaccag catctcgaaa aagttaatta ctcctctcaa
      361 tatagggatg ttcaaaatct tgtatctttt agaaaaggca acataatcct ggcccctcac
      421 ctcaattccg ccatcgggtt tgcggatagc agtggatatt ctccaggggg agcgtatcat
      481 aaccccttcg atcaccgctt ggcctccaac tttgggcttg gacatttttc tccaaaaaat
      541 atcccgacta agaaggcgca aagcctcatt agtcgggata aagtcctaac atctactgct
      601 ctttcttgtt ctcctctttc ttgccatatt tctttatgaa tctttccacc cttccagcag
      661 aatcaatcaa cttctgcttt ccggtgaaga aggggtgaca ggcggagcag atttccacat
      721 ggatatcctt gactgtagag cgggtattaa tcacattccc acaagcacaa gtgatggtgg
      781 tttcaaaata ttgaggatga attttctcct tcacaaaaat cgcctcctaa atggtttccg
      841 taaatataat acgtcaaaga ataaaagcaa ggggaaagta attttggggc acatgaaaga
      901 acctaacccc ctatcgtccc ccttgtcagg gggacagagg cgttcctccc ctgataagtt
      961 tagtaggtcg ggttgggagt tccgcctcag cgggagagtt acccgacact tcgctacgaa
     1021 aaacagtagg agcaattcat gaattgcccc tacgcactca tttcctgttg taggcggcat
     1081 tcggcactgt cgcaaataga ccttgagata ttctttggag tgcatcgtcc gtgacgatgc
     1141 agcattgacg tggtcaatgc actccatata ctctatcctg agcttgtcag aagatggagc
     1201 ggttttaaaa aaccgtctaa tgaattaggc aactacaaag aatcggtctg cctaattctt
     1261 aagccaatgg ggttcttgtc cgataatctg tatgtagata cttaaattca tatataagga
     1321 gggagttatg gaggagaaat tctattattg caagtggttt attggcttgg ccatattcga
     1381 gtttattatt agtctaggga ttggttttat gataatacgt gacgctttgg acaatctgtt
     1441 tatatggcat tctggggata ggttggggtg gattgtggct gcaatctctg gtttgttgtt
     1501 gatagttgtt gggctagcat tcttgcgatg tgtggcaaag ccagcaatca taatgacaca
     1561 gatgaaaatc gcttttttcc cactttccat aaaggcaaac tggaagccat acttttttaa
     1621 cctgcgtaaa cttgaactcg gcgttgaaca gattgtctcg gctaggataa cggaaatgcc
     1681 ctcagtgtat ccttttgaaa agccgtataa agtggttgag gtcaagttca gacgaccaag
     1741 tggcgtagaa gattcttttt acatcttcac cagcaaaatc gcaaaaaaag atagattaat
     1801 cgatatcctc aagcaacaca tcaaatttga ataaaggcgt agggacaggt tccacgcttt
     1861 tagcgggatg ttccacgcga cctgtcccta cagacttttg tatggagaca tggtgtggga
     1921 tgtggttcaa caagttcacc atcctgagca aagtcgaagg aaattcccac acctacgaat
     1981 attttattcg tagcacaggg aatttatccc tgtgcgtatt tgcatattat tccacggtca
     2041 cgctttttgc caaattcctg ggctggtcaa cgttgcatcc gcgcaggact gcgacatgat
     2101 aagccaaaag ttgcaggggt ataatagaaa gaagcggagt taaatgctct aaggtctggg
     2161 gtatataaat cacatggtca actttcttag ctatttgtgt gtccccctct gtcgctatgg
     2221 cgataattga acccttgcga gcttttatct cttcgatatt ggaagacacc ttagcataga
     2281 cactatcttt taaggcaacc accaccaccg gcatattctc gtcaattaag gcaatgggac
     2341 cgtgcttcat ctctgcggcc ggataacctt cagcgtggat atacgatatc tccttcatct
     2401 tcaaggcacc ttccaaacca accgggaagt tgactcctct gcccaagaag aggaagttgt
     2461 ttttcttgta ataatgtttt gcaatctcct tgacttgccc ttcgtttttc aacatccact
     2521 ccacctgctc aggaagcttt tgcatagcat ttattatctc ccgtccctgt tccaaagaca
     2581 tatctctcat cctgcctaaa agcaaggata aaagcgaaag caccgccatc tgggcggtga
     2641 aggctttggt cgaagccact cctatttccg gaccggcatg ggtatagact ccgccgtctg
     2701 tttcgcgcgc aatggtgcta cccactacat tgcatattcc caagaccgta gctccctttc
     2761 ttttggcttc ctttaaagct gccaaagtgt ccgcggtctc cccagactgg ctaatcacaa
     2821 acacggtggt tccatcacgg attatggggc tgcgatacct gaattccgaa gcatattcca
     2881 cctccaccgg gatgcgagca tacttctcta tcacatattc acccaccaaa gcagcatgcc
     2941 aggcggttcc gcaggcggtc agaattatgc ggtcgattaa gtggagctgc tcgtattgca
     3001 ggttcagtcc gtttaagcgg gcagttccct cctcgtaatt caatcttcca cgcatagcat
     3061 ttcttaaggt ggtaggctgt tcgaagatct ccttgagcat gaaatgaggg aaaccctctt
     3121 tctctatcat atccaaactc caggatatct cttgaatgtg gggagtaacc ttgacattat
     3181 caatagtgga gatggagaag ccatctttgg taatggaggc taactcctta tcctgcaaat
     3241 agaccacctg attggtgtgt ctcaaaatag cagccacatc tgaagccacg aagttctcat
     3301 ccttgccact tcccaagacc aacgggcttc cgtgccgggc agccactatc tcatcgggat
     3361 tgtctttaga aatgactgca ataccataag ttccttcgac cagagaaagg gcggagcggg
     3421 ttgcttccac cagactcccc tcataatact cctcaatcag atgagcgata acttcagtgt
     3481 cagtatcaga tatgaactta tgcccctttc ttaagaggag ttcctttaaa ctgtggaaat
     3541 tctcaatcac tccattgtgc accactgcga tctttccgga acagtcaacg tgcggatggg
     3601 cattcaagtc agttggctca ccatgggttg cccatctggt atgggctatg cccaccgtgc
     3661 ctttgaagtt atcatctttt aacaaagttt ccaacgcaac aatcttgccc gcggtcttct
     3721 tggagagtag ccctccctct tccaaaatag ctattccagc tgaatcatac cctctatatt
     3781 ccaatctctt tagcccttcg agcaagatcg gtacagcttt ttttgttccc acataaccta
     3841 ctatgccgca catcttttta tctccttttt ttcaagttaa taattccaaa ccttccttgg
     3901 ctaaatcttc cgcagttttc ttggtcttag cttcagctat gattcgggcc gccggctcgg
     3961 tatttgattt tcttatatgt agccaactat ccttaaagtc aatccttatt ccatcctttt
     4021 tatttatcct ctcctttttg aatctcttga ttaaaccttc aagtctatca tcaaaatctt
     4081 tcgatacttc gattttcttc tttagcatga agtatttcgg catatcctgt atgatctggc
     4141 tcaattttcg atttgattct gccaagtact ccacaatcaa agccattccc accagagcat
     4201 ccctggtata atgcagttct ggcaagatga ctccaccatt tccttcccct ccaattactc
     4261 ccttaatttc tttcagtttc ttggcaacat tgtattcccc tactggagtg cgatatactt
     4321 tacatcctga tttttctgcc acttcttgag tcattctgga agtggaaagg tttataacca
     4381 cttccttggg atttttgctt aagataaatc gggtagccaa agccagggta tattcctcgc
     4441 tcaaaggagt tccttcatct gccaccaggc ttaatctatc tccatccgga tcaagacaga
     4501 acccaacatc agcatttttg tctctgacca cttttgccag ggacaaaagg ttttcaacag
     4561 tcggctcagg tgcatgagca aaacttttgg aaaaatcgca attcagctca actatcttgc
     4621 accccagttt tcttaagagc aaaggagcaa ttctgcttcc tgctccatta ttgcagtcaa
     4681 ccaccacctt taaattagcc tttcttattt tgtccttttg tacaaatccc agttttagga
     4741 tatagtcaat atgtttctta taccaatctt cttttggaga taatttacat aaaggaactt
     4801 tcttaatatg acttatattg gttttctgga ccaaaagatt caatttttcc atctttttct
     4861 tattcagaaa agttccgtct ttttcaacaa acttcattcc attccactgg aaggggttat
     4921 ggctggcagt tatgcagatt cctccaactg ccccgagttt ctcagtggct aaagtcaaag
     4981 tcggggtagg gcaaattccc aaatctacaa catcgcatcc aactgacaac aatccagata
     5041 ctgcagcacg tttcaaaacc tttccagaaa ctctggagtc ccttgccaca accacctttc
     5101 ccttatggca caagatccca aaagcaagag catatcccag tgcggtttca gggaaaagag
     5161 tctttcccac cactcccctt aaaccggaaa ttccaacaat tagtttatcc ttcatatcaa
     5221 ataaaatatc aacaagagtg ccttgaagca atacaaaact gccaaaatgg gtctccaatt
     5281 atataatcgg aaggaggata aaagaatatc accctcccat taatcctctt ttggctgaaa
     5341 ccatatagat gcctgatatt accaaagccc caccaatcag aagccaaaaa gtgattgcct
     5401 ctcttagtat caaactcgcc ccgattgtgg ctactaaagg ctccaaatag agataaacgc
     5461 tgacttcgcc cgcatttcca cctttgagcg cctttgacca gaaaagatag gcaattccgg
     5521 agcagaagat tcccaaataa gcaatagcca tccaacctac tttcgaaagc tctaacacag
     5581 aaacaaagga ttggtggttt aaactcaaag gaagagtcag gatacagatt atagccagag
     5641 aatagaacgt tatcaaagtg gccgaatagg tatcgagcag ttttttgctg gcaacagaat
     5701 aaatcgccca agtgacactg ctgactaata tcatccagtc gcccggactt cgaatgaagc
     5761 tgatgttttt tggttccccc ttgcttacca gaaatatgac tcctaaaatg gcaagcgtaa
     5821 tcccaactat cttcaagaag gaaaacctct ccttaaatac aagacgagat agaataacta
     5881 caaacaccgg tgaagtggca acaatccagg cggtgttgga ggcagtggtg aacctcattc
     5941 cactgacctg aaccgaaata tgaatagcga ttataagacc acaaaagagg agaagaaaaa
     6001 tatcagattg ttttatctta agaggatatt ttttgaaggc aagcgcccca gccaaaatca
     6061 aaacgccgat aacaaatctc aataagatca aaatagcggg cgatagctcc cgtagggcga
     6121 ttttggtggc aacaaatgat agtccccaga agattatagc aaagagagga tagattagca
     6181 tgttaaggaa cagatttatg aaacatgtgt cagaataccc ccctaggggg atgaatgacg
     6241 tttctataag ttccctctcc cttgggggag agggttagag cctgccctaa agcgaagcga
     6301 aggggtgagg gggatagcaa acattatctc accctctctc cgcctttggc gagattttca
     6361 acttagtcct ccccctcaag ggggaggaga tgttttgaac gcccccttag ggggaagttc
     6421 actgggaagg ggttatttgc tttaaaacgg ccagcttatg atcggtattt tcagatgaag
     6481 aggattcatc catttcaata aaaagattat ccttcactat caggagaaac gaagcatacc
     6541 ccggatagtc tggactcaac tcttccagat ttatcttctt gggcttgctc tcagagatgt
     6601 aatatccgaa ggagccctcc tccttctgtt tgaaaatttc atgggggagt tctaagattt
     6661 tactatcctc ttcagactta taaaaaatcc agacatcttt tggagaataa aaggagatta
     6721 agtcaacttc cagataggag aaatccttat tcttttttat ccaatgttgg attcttctcc
     6781 aatcttttat cccttcagtg cttttctggc gatcggaaat ataaattcca ttgctgacca
     6841 ataagtcatc cttgctgttg gttaagagaa gtcggggaag gtccacaaac tttagtttgg
     6901 agaattggtt atccaggaaa tagttaatcc ggttcttttc cctgaggtag cccacattat
     6961 ccataattcc cttcaagacc agcaaagcca caaatattat aactaagatg aaaaataccc
     7021 ttacaacttt tatcatattt gttctatttt ctcctttaat ttttgggcta actgcgggtc
     7081 taagctttta agttgctcat attgctcttg ggctttctct ttatctcctt tgatagtata
     7141 aacatatccc agattgtaat aggctttggc gtggagcggg gtaaagcgga tgactttctt
     7201 ataagactct atggcttggt catacatccc cttctcagta taaagcacgc ctaaattgag
     7261 atgtgcctgg acgtaagttg aatccgactt gattgccttt tgatattcca atacggctga
     7321 atcggagttg gccatcaatt gatacctgac tccttttttg aaatggtctt ctgcggtctg
     7381 cctggagcaa ctgaaaatta aaaaagaaaa aatcagaaat gaaaaaattg catacttttt
     7441 cataaaacac ctccctttta atggaaacgg ttaaaggata tcatttcaga taagtttttt
     7501 cgcggtcggg gttttatccc agccatccca tcttggttgc tttcgtccgc aggataaccc
     7561 aagggcaaaa acataaccac atcccacccc ttaggaacac ccaggatttt cttcgtcttc
     7621 ttttggtcaa accaacccac ccagcaactt ccaattccca aggaggttgc catcagaacc
     7681 atatgctcta tggcaatgga gcaatctatc ctatgataat cacttgctac gattttggca
     7741 agctgatgtt caattgcatg cggcttggct aaacaggcga ttacaatcgg cacattggaa
     7801 aaccagatat tgggtcttat ccccagaggc accgcaccaa ccagtttctt cataacctcc
     7861 ttatccttta tcaccacaaa atgccagggt tgggaatttg aagatgaggg tgccaggcgg
     7921 gcagattcta aaatcagatt taatttgtct tcctcaattg gtttatccga gaatttcctg
     7981 atgctgcggc gatattttat aagattaaga aggtcttgag aattcatttt atattacctt
     8041 taggttgtca tccccgagtg atttaatcgg ggatccattc cttggattgc tgtaattaca
     8101 tttaaacggc aatgcagggg cgggtccaaa agagtatttc agttcactta aaggtttctt
     8161 ggctgtcatt gcgaggagtc tcgcctgtgg cggacgacgt ggcaatctct tacaacatca
     8221 taggttataa aatcgagatc gccacgctct cccgataaag tcgggatcgc tcgcgatgac
     8281 aataaacttc tgggtcaacc ccctatatct ggattcctgc tgcagtttat cccctacggg
     8341 gacaggaatg acagataaat tcaaattaac aattctctct gcaggcgacc catatgggtc
     8401 gcctgcaaat ctcattaaag tgcattgata ttcaaactgg aggcggtggg aatcgaaccc
     8461 acgtccgaag acaaagattc aaaaccttct acatgcttat ccggtttatt tttattttgc
     8521 cccagggaac accaaccagc aggttttccc caaggctagc ttagatttaa ttttcgccca
     8581 aatcccccaa gcgagaattt agactattcg gtttatctcg acgccaaaat ttgagccagc
     8641 cgacaagccc aaacctgacg ggctaccttt ttttaattag gcagccagtg catacgcata
     8701 atcgtttgca cttgaatttg tcccaatgga ttaacgaggg aactgggtac ctcggcatgc
     8761 cagtcctgcc cttctatccc cgtcgaaacc atatcgcccc cggaaattca gttcacagtt
     8821 cacggttgac agttcatagt aaataaaaca aagaatgaac tctaaagata aagttcacgg
     8881 ttaacggttc acggttcata gtatgaaaac caaaaaacaa aataggtaaa catttcgcag
     8941 tcaaaaaatc aaagagcaaa ttccatacat aatatacggc agaaaaaaac caagtcaata
     9001 ggacaaattg attatttgtt agtccataaa ttacaaagag ttaaacgact caataaagat
     9061 tcgattggca aacaaaaaag attcaatgag ggatttgtaa gaaagcaaaa attttgagaa
     9121 aaacaaagag gcaggattga aattttcctg ccgcttttgt ctttctcttt tagggtcgta
     9181 tttattccgg cttgatgggc ccgctggacc cctcctgacc tccccttatc aggggaggaa
     9241 cgatttttgc cccgtgccat cagaagaggg acattaccta tccccctgat aagggggata
     9301 acagggggtt acgttttttt gcaaccgccc cgaatgaatc gggatttcgc tccgcttaac
     9361 aggggggttg cccctgtatt tcttagcaag gtgaagggcc acctttgaac aggaaattaa
     9421 tcagatatat cacatccgag acagtcacct ttgcgtcaca attcgggtct ccggcttcca
     9481 taacagggac gggtgcaggt ccacctttga acaggtagtt aatcagatat atcacatccg
     9541 aaacagttat ctgcttatca gcattggcat cgccatgcct gaagagtgtg gtggcatcgg
     9601 ctgtagcaaa ttcatcgcta tattgtgata attccccttc tgaggaatat gaccacgctt
     9661 gataatagta gtgagttaaa ggcaccaagg tggaatcctg gcaagttgtt cctgcatcat
     9721 tataaacttc tgttccctct ccccttgtcc agcttgttag agtgtctctt tcgataaccg
     9781 tcctatctgc agatgaaggt ttagtccagg agagacttgt tttattccag gcaactcctt
     9841 gtgcagtcag ttcagtgggg gccaagggtt tggtcaaaaa cgccagctct ccaccactac
     9901 cccaaccagc aatattaaat cctttagcaa tgtaataata agctgttcct tctgtcagac
     9961 cggtgagatc tttgctgaac tctgaagcgt tggggaaaac tcccagagat tcgctattgg
    10021 cataaggcac tacagaatca gtatcccaga agagccagca ggttgcctca aatccgccat
    10081 cattatccag ataaccatgg atagtcgcac cggtctcttc aacctccgta gctgggtcag
    10141 ttctgaccac cgggctagcc acactggctg aatcctgaca agggaggtag tcatgtttag
    10201 ttacagtcac atacatgatg ccctgggtag atggtgaggg attaaaggtt atatttccac
    10261 tggtattggt atttcccttt agataaactt cactcccttt ccacaggcag acgtaagctt
    10321 gatatacagc gctgtgtgtg gttgaatcct caacatgcac caaaaacgag gaacttccaa
    10381 ttggaatagc gtgagagaag ctcactgcga aactatccgg ctcatctgtc cagataggca
    10441 tctccggctc acccaaaaga ttgaatgtcc attcgcaatg cttctcgatg ttacctggag
    10501 tgctgaagtt gtgtttgctg tccgcaagag cttgacccaa gttatactta ttgcggttga
    10561 aaagcgcggc ccaccactgc atatccaggg cagcacttaa ggtgtaaggg tcgccttgag
    10621 tataccatcc gtcgcgagtg ttgccagtga aagccactcc tgcctgattg gggttgtatt
    10681 cgacaaaggt ttcagcaatg caatcgccgg catccatttt attgggaaga caaccagtgg
    10741 aaactattat agacatttga ttgtcattgg tcagattatt tacatctgct tgactgagca
    10801 gcaggttgtg gttaacatat cctgtgccca tgacatcata attggaatgg tcagaatgat
    10861 ttaccaggtt ctgtccggcg ttgagggcag cgatagttgc agttttgtgg ttggtaccat
    10921 cactatcgta caccttagtt acgttgaagc gcgagggaat ataaacggct tccttcgtat
    10981 cttgactggg cgtgctggca tccaaatcca ttccgacaag caaaacatcc agagggtagt
    11041 ctgttcgagg gggatcagtc tcatatttta gaaccttgtt aacgaaagta gtaaccgcag
    11101 ttgagcttcc tgcagggatt ctgcccacaa agacctcgtt agtccaatca tcatcataat
    11161 cagaatagta ttggtcgctg ggcgtactct ccccgttata gtaggttcgg tattcaaagg
    11221 gaacttcttc attctcaccc ccaagaagaa aataggttgt gccccaggtc gtgtgcgcgt
    11281 cttttatgaa gttgcggatc ttctcctggt tggtggctcc gctgtatgcg gcataaatat
    11341 catctgtagt caccaccgta tctttcactc cctttttagt gtgccacctg accagaggtt
    11401 ggaaggctga tacgcggtag gtgtgagcga tgatggcatg accaaaggag ccgggaggga
    11461 ggagagtggg agcaccgatt gccatgcctg tgttcaaatg gacatcctcc gggttgatca
    11521 caatatcttt gaccatctgc tcataggttt ccctggcttg gtccgatata ttcggcggta
    11581 agtaatcacc atattcatat cccccagtac cttcaataac caagttaagg gaagcgtaaa
    11641 gggtaagttt cttttcagct ggaatgtatt gtaagggata aaccctgatt atagctatgc
    11701 tctgaccagc caaatcagtc tgcctaagga actccaccag ttttgaaggg tatggctggc
    11761 tggaggcata aagtgcctgg tcaggttgca cggttattat ctcttcatcc gaggagctta
    11821 tttttttcgg gggttgggct ggaaagatat tgtattcgcc gatgatttcc tgggttttaa
    11881 tatccgtggc ataaacatct gtcaccttca ttccagctgg cagtgctatt cttaactctt
    11941 taaccggtag cataggtttg gcaatatcac tcaaccaatc accacctttg agcctcaccc
    12001 tatcatatcc tgccagctta tcaaatgaga ggtcactttg gacaaattca gccacatact
    12061 ttgtattata gctggcttgc gacaaaagag ggctagcgaa aagtacaagc gagagtgcag
    12121 caaagaggcc actaactaat cttgttttgg tcatttttcc tccttagaat tctaaattga
    12181 ccttattact gaagaaaacg aaagatatgt tattcttctg ttcttccgca ttcaatttaa
    12241 gaaaaacccc agatttgtca acagtgtttt tacaaaaacc aagataatag ataacttctg
    12301 agtttataag ctcaattcat tgatacggca ctggggatat ttgagtttga tacgtataat
    12361 tgagatgcaa gagctagatg gatatcagtt cttaaatacg acaacacata aacaacttga
    12421 caaagagttt agagtttatt tgattctggg caaggcaaga aggaaagcgt ggctggttgg
    12481 aatcaaaatt aattactaaa tcaaataaca aaggaaggcg atatgcccaa acttacgcga
    12541 agagagtttc tggaaatggg agctaagggg gttgtcattg caactattcc tatcattttc
    12601 aagcccaatc cggctatagc atttttaacc cctccagagg gaaagggtaa gctgaacgat
    12661 tattacaagc atttcggggt ggatgagaaa actatccgtg agattatggc tactgcgctt
    12721 gagcgaggcg gggattattg cgaccttttc tttcagcacc gcatcaccaa ctcggttggc
    12781 ttagaggata atgcagtgga tcgagcttat accaacgttg actatggagt gggcatacgg
    12841 gtgatcgaag gggaacaaac cggctattcc ttcactgaag aaactacttc taaagcgatg
    12901 aagttagctg ccaggacagc cgccaatatc gccagccaga acaagaccgc ccctccgggt
    12961 gggctaactt ttcaccagac tcctaattat tatcccatcc tgacgcaatg ggaagatgtg
    13021 ggggttgacc agaagattcc ctatctccaa acaattaacc agaaagtatt tgccttagat
    13081 aatagagtaa ttaagtctca ggtctcgttt tcgaacgaga gctcgtatat tcttgtggcg
    13141 acgtcggacg gaaagatagc ctgcgattat cagccaatga ccgcaatctc ggtaacgtgc
    13201 agtgcggagc agaatggcag gcgggagcaa aacggcttta acctctctgg tcgccaggga
    13261 atagagtttt tcaccccaga gaacctcgac cgtctggcaa aggagtcggt ccagcgcacc
    13321 atcgttatgt ttgatgcaat caagccggaa ggtggagaaa tggaggtggt gcttgctccg
    13381 ggcaggtcag gcatacttct gcacgaagcc attggacacg gaatggaagc ggactttaac
    13441 cgcaaaggca tctccatctt ctcagataag ataaataagc cagtggctaa agagttcgtt
    13501 tcgattgtgg atgacgggac aattccaaat tcgcggggtt cgatcaatat ggacgatgaa
    13561 gggaatgaca cggagaagac ttacttggtg gagaacggaa tcttgagaag ctatctgcac
    13621 gaccacatca gcgcaagaca ttataaggtt aaaccgactg gaaacgggcg cagggagtcg
    13681 tttcgctttc ctcccgtacc caggatgaga aatacctata tgcttcccgg ccctcacaag
    13741 agggaggaga taattgggag tgtgaaaaaa ggattatatg ctgagtcgtt caccaacggc
    13801 gaggtccaca tcggtgctgg agatttcacc ttttatgtta agtccggatg gctgatcgag
    13861 gatggtaaac ttacccgacc ggtaaaggat atcaatatta taggcaacgg accgaaggtg
    13921 ctggagaata tcgttatggt ggcagatgac cttaagctga gtgaaggagg aggaacttgc
    13981 ggtaaggatg gtcagtccgc gccggtctcc atgggcttac ctacagtgaa ggtctcctcc
    14041 atcacagtgg gaggggtatc ttaattcaac accttaaaaa tcgaaaacga ggttcaaact
    14101 tatgaacaaa aaagaaagat tggacctggc caattgggca gtcaaccaag ccaagaaaag
    14161 tggggccgac caagcggcaa ttgatgtctc atataacaga aatgttgaag tggggttccg
    14221 cgaaggaaaa atcgaacagc tgaaagaatc agaccaaaac tccttgagct tgtcggttta
    14281 tgcgaataga cgctattcca gccatcgaac taacgacatg cgtaaagatt cattgagtaa
    14341 attcgtccag gaagcggtgg cgatgacaaa gtacctaaat gaagatcctt tccgcttgct
    14401 tcccgatccc aaatattatg agggtgggaa aaaaacgaac ttagagctca atgaccctgc
    14461 ttatgagaag gtggaatcaa ccaagagggt ggaaatagcg caaaagatag aaggggccgc
    14521 tcgagcccaa agcgacaaaa tcatctcctg cacctccggg tactctgata actattatga
    14581 atccgcaaag gtgaacagca atggtttcga aggtgaagaa tatggcacct tcttttcagc
    14641 cggcgccgaa gtgacggtga atgaccaaag gggaaggcgg cccgaggatt gggaatgggc
    14701 gactgctcgc ttctataagg atctgccctc gccagaaggc ttagggaaga aagctagcca
    14761 gcgcgctttg gccaagatcg gacagagcaa gttggagtcc ggttcatacg atatgattct
    14821 ggagaaccgg gttggcggga gatttttgta ttctttcttt ggacctatgt cgggcaggtc
    14881 gcttcaacag aagagttcct ttctggatgg aaagctgggt caaagaatcg gctcggacaa
    14941 actcacccta attgacgatc cgtttatagt ttccggtatg ggttcaaggt tatttgatga
    15001 tgaagggatg gcaaccaagc gtcgcgtgat gatcgataaa ggaatcttaa aagcatttta
    15061 catcgattcc tactatggca ggaaactggg catggacccg acatcaggct ccgcaagtaa
    15121 tgtggtcttt gattacggca cagaatcgtt ggataaattg atcgaaggga caaaaagagg
    15181 tattctggtc aatactttca taggaggcaa ctccaactcc accaccggtg atttctctta
    15241 tgggatcatc ggcatgtaca tccaggatgg gaaagtcata aaaccggtaa atgagatgaa
    15301 tgtcagcggt aatctggcgg agctgttgaa tcaattggtg gaagtgggaa atgaccccta
    15361 tatttactcc tctttgcgca cacccactct gtgttttaag gatgtccagt ttagcggaat
    15421 ttaagtttgg gtgctatagc caaattttta tgcctgttgt ttacggaaaa atgtatcctg
    15481 aggttggatc agctggtcta ttgaatcgta gattcaaagt tttgattgaa ttaagagatg
    15541 acaaaga
//
LOCUS       DASWLN010000022         6218 bp    DNA     linear   ENV 20-FEB-2024
DEFINITION  MAG TPA_asm: Candidatus Zixiibacteriota bacterium isolate
            SMAG_U2949 k141_655524, whole genome shotgun sequence.
ACCESSION   DASWLN010000022 DASWLN010000000
VERSION     DASWLN010000022.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37960594
            Sequence Read Archive: SRR10376091
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Candidatus Zixiibacteriota bacterium (soil metagenome)
  ORGANISM  Candidatus Zixiibacteriota bacterium
            Bacteria.
REFERENCE   1  (bases 1 to 6218)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 6218)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (26-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/12/2024 08:28:26
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 1,440
            CDSs (total)                      :: 1,416
            Genes (coding)                    :: 1,412
            CDSs (with protein)               :: 1,412
            Genes (RNA)                       :: 24
            rRNAs                             :: 1 (16S)
            partial rRNAs                     :: 1 (16S)
            tRNAs                             :: 21
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 4
            CDSs (without protein)            :: 4
            Pseudo Genes (ambiguous residues) :: 0 of 4
            Pseudo Genes (frameshifted)       :: 0 of 4
            Pseudo Genes (incomplete)         :: 3 of 4
            Pseudo Genes (internal stop)      :: 1 of 4
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..6218
                     /organism="Candidatus Zixiibacteriota bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_655524"
                     /isolate="SMAG_U2949"
                     /isolation_source="soil"
                     /db_xref="taxon:2053527"
                     /environmental_sample
                     /country="USA: Oregon"
                     /lat_lon="42.99 N 123.63 W"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            complement(<1..614)
                     /locus_tag="VGB16_00685"
     CDS             complement(<1..614)
                     /locus_tag="VGB16_00685"
                     /inference="COORDINATES: protein motif:HMM:NF015948.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF362 domain-containing protein"
                     /protein_id="HEX9916226.1"
                     /translation="MKSRVSIVRCEDYSPESVKAAIKKTFENLGGLESFVKPNQKVLV
                     KPNLLVAKDPGRAITTHPAIVQSVVEEVKKLKGIPIIGDSPGGAERGVKRVWENTGMW
                     EVGEKTGAELVKFEGGGICKAIASNSKVYYISKHAVQADAVINLAKMKTHVLTLFTGA
                     IKNMFGMIPGVRKAEYHRQLPHPKEFSQMLVDIFSLVKPKLSIM"
     gene            complement(936..1484)
                     /gene="ybeY"
                     /locus_tag="VGB16_00690"
     CDS             complement(936..1484)
                     /gene="ybeY"
                     /locus_tag="VGB16_00690"
                     /inference="COORDINATES: protein motif:HMM:TIGR00043.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004540 - RNA nuclease activity [Evidence
                     IEA];
                     GO_function: GO:0046872 - metal ion binding [Evidence
                     IEA];
                     GO_process: GO:0042254 - ribosome biogenesis [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="rRNA maturation RNase YbeY"
                     /protein_id="HEX9916227.1"
                     /translation="MPRRTKISVENLHHNCKPDKNKIASLAKRILSAHRSNLDLDIIL
                     VKDQFIQQLNKNFRRKDKVTDVLSFGMEEGKKIGLELNYLGDVYVCLDQAKRQAKEYG
                     IPFVEEVYRLVTHGILHLLGYEHKTKKQTERMKSEEDLFIAQITNMRPLKKYGRVVGQ
                     ALNRAGTGQGARPILRILATKN"
     gene            complement(1468..3696)
                     /locus_tag="VGB16_00695"
     CDS             complement(1468..3696)
                     /locus_tag="VGB16_00695"
                     /inference="COORDINATES: protein motif:HMM:NF019317.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="HDIG domain-containing protein"
                     /protein_id="HEX9916228.1"
                     /translation="MVFEFFLKTRRKIKKFVFRKTDNDKKGRVNQRKHYLFKVVLALV
                     VIGIVTFIYPSSSVYRPLQTPQEGEAAYEDIISPRNFQILKSPEEMEQDRQMIRANQP
                     LILRYQDKVFDSVMVELKRFFSKVDSLNQSIPDTTLRLQQFALQFPNLSQESQIALSH
                     EPNVKVIKRFLTTVLTEIYDSGFVAKADDLPLNRVKTAAVFRKNGEHILTQKQLLDKV
                     KAEEGILSSATAKFKEAPLLAKGTYDISTQFLIPNLKMDVEETKGKIKEETSLISPYK
                     GLVLKGERIIGKNEKVTKEHLEKLESLAYAEHESRSTEAVLPYVGRALFVTFVFLFLG
                     LFLYFFKRKIFNNNPKLLMICLIILLEIFLSLVLTFKLGWPQYTIPVVIASLLLTILI
                     DVEVGLVSTVALGLLLGVVHNFDFKLTFLTIVVGTLGCYSVKEVQKRYRFYRPMLYIS
                     FAYIVLIFFMEYLRHTSTNEILELCGIGLANGFLSILLSIGFLPIFESLFNITTDISL
                     LELSDLNHPILKRLTLEAPGTYHHSIVLGNLVEAAAKEIGANTLLARVGSYYHDIGKI
                     KKPEYFVENQMGAKSKHEKLAPTMSALILESHVKEGVELAEEYNLPDAIIDFIQEHHG
                     TSVMTYFYSKALEQGATEEARDEFRYPGPRPRSRETGIVLLADAVEAASRTLEDPKPS
                     RIKSLIRKIIMDKFEAGELDECPLTLKDLHAVEDSLLPILIGVFHPRIDYPEVEEDAK
                     TD"
     gene            complement(3711..4691)
                     /locus_tag="VGB16_00700"
     CDS             complement(3711..4691)
                     /locus_tag="VGB16_00700"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_015327515.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005524 - ATP binding [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="PhoH family protein"
                     /protein_id="HEX9916229.1"
                     /translation="MEKLNTAKSIDISGVDQRLLFGQNGVLLNLIESQFDAKIVARGD
                     VIHLEGEEKEVEQLCVLFRDLISHLKKNGELSQRYVLYAVSMVRQSGVGPFNEMQIAG
                     LMTSPLTKEEIKPRTLGQKEYVEAIEKYDIVISIGPAGTGKTYLAVAEAVSALKKKRV
                     GRIVLVRPAVEAGENLGFLPGDIRAKVDPYLRPVYDALHDMLVMEKIRKLMEMGVIEI
                     APLAFMRGRTLNNSFVILDEAQNTTTAQMKMFLTRLGTNSKAVVTGDITQIDLENKKA
                     SGLVQSQKILSGIGGIKFVYLTEKDVVRHKLVQDIIRAYEKYEHRGRKSK"
     gene            complement(4861..>6218)
                     /gene="aspS"
                     /locus_tag="VGB16_00705"
     CDS             complement(4861..>6218)
                     /gene="aspS"
                     /locus_tag="VGB16_00705"
                     /EC_number="6.1.1.12"
                     /inference="COORDINATES: protein motif:HMM:NF001750.0"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0000166 - nucleotide binding [Evidence
                     IEA];
                     GO_function: GO:0004812 - aminoacyl-tRNA ligase activity
                     [Evidence IEA];
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_function: GO:0016874 - ligase activity [Evidence IEA];
                     GO_process: GO:0006418 - tRNA aminoacylation for protein
                     translation [Evidence IEA]"
                     /codon_start=3
                     /transl_table=11
                     /product="aspartate--tRNA ligase"
                     /protein_id="HEX9916230.1"
                     /translation="KAVKAVRDYLDEQGFYEIETPLLIRSTPEGARDYIVPSRVNKGR
                     FYALPQSPQLFKQILMMSGFDKYFQMARCLRDEDLRADRQPEHTQIDVEMSFVTQDDV
                     FEVVQKMMAYIFKKVLNVEVKIPFPRLSYNETYTKFGTDKPDLRYQMEVCDLSDIVEN
                     SQFKVFSDTIKGNGKVCGIVFNGGGTLSRKELEKLEEMAKQSKAKGLSWIVFSSEGFK
                     SPILKYISQEEMKSIIDKMKAKIGDLILIVADKPEIVYPVLGKLRTEIARKYNLIPKD
                     KWSFEWIYQFPLFEYNPEEKSFQAMHNIVTMPYEEDIPKLDEGFSSKLPLDDRNHPWA
                     KIKANQYDLVLNGVEIASGGIRNHKREIQEKLLKVLGMDKNQAEKRFGFLLDALEYGA
                     PPEGGIALGMDRIVALMTGTESIRDVIAFPKTTAAQSLMDGSPSEVEEKQLRELGIKL
                     I"
BASE COUNT         1618 a         1477 c         1190 g         1933 t
ORIGIN      
        1 tccattatgg atagtttcgg tttgactaaa gagaagatat caaccagcat ttggctgaat
       61 tcttttggat ggggaagctg cctatggtat tccgcctttc taactcctgg aatcatcccg
      121 aacatatttt tgattgcacc ggtgaataaa gtcaaaacgt gggtcttcat cttggctaag
      181 tttatcaccg catccgcctg cacagcatgt ttggagatat agtaaacctt gctatttgaa
      241 gctatcgcct tacaaattcc ccctccttca aatttgacca attccgcacc agttttctct
      301 cccacctccc acatcccagt attttcccaa actcttttta ctcccctttc cgctccaccc
      361 ggactatccc caataattgg gatacctttc agttttttta cttcctccac gaccgattgg
      421 acgattgcag ggtgagtggt tatggctcgg ccggggtctt ttgcaaccaa aagattgggt
      481 ttaactaaga ctttttggtt gggtttgaca aaactctcca gccctcctaa attttcaaaa
      541 gtctttttga tcgccgcctt aacactctcc ggagagtaat cctcacatct tactatcgaa
      601 actctacttt tcatttaggt tcatctcttc atattgagat tactgaaaaa cccctttcgc
      661 agggccagtg gcttcgccct cggcttcgcc gagaggcgag cgccgagagg cgagcaccta
      721 ggcgagctgg gagccctgcc tacggtatga accgtatggt tcatacctac gggaatgcac
      781 gaattatgtc cttcgacctt gcccttcggt ctgagctcag ggtcgaagac tcaggacggt
      841 gaggacgttc ggctgagcgg ttcgaccgtc ctgagcggct caccgtcgaa ggactcagtc
      901 gaagtccttg tcgaaccgtg tcttgagata agtcattaat ttttcgtcgc caggatccgt
      961 aggataggtc gggcaccctg ccctgtacca gcacggttca gggcttgccc gaccactctg
     1021 ccatactttt tcagcggtct catattcgtg atttgggcaa taaagaggtc ttcttcactt
     1081 ttcatcctct ctgtttgctt tttggtttta tgctcatatc ccaagaggtg caagattccg
     1141 tgggtcacca atctatagac ctcttcgaca aaggggattc cgtactcttt ggcctgcctt
     1201 ttggcttggt ctaaacatac gtagacatca cccaggtagt tcaattctaa cccgatcttt
     1261 ttcccctctt ccatcccaaa ggataatacg tcagtaactt tatctttcct tctgaaattt
     1321 ttattaagct gctggatgaa ctggtctttg accagaataa tatccaggtc aagattcgac
     1381 ctatgcgcag ataaaatcct tttggctaaa ctggcgattt tgtttttgtc tggcttgcaa
     1441 ttgtgatgga gattttcaac tgagatttta gtccgtcttg gcatcttctt ccacttccgg
     1501 gtaatcgata cgcggatgaa aaaccccgat taatataggt aggaggctat cttccactgc
     1561 atgcaaatct tttaaggtaa gagggcattc gtccagctcc cctgcttcaa atttgtccat
     1621 aattatcttt cggattaggc tctttatgcg cgatggtttg gggtcttcca gagttcgtga
     1681 ggctgcttca actgcgtctg ccagaagcac tatgccggtt tccctggaac gcggcctggg
     1741 accgggatag cgaaattcgt ctcttgcttc ctcagttgcg ccctgctcca aagccttgct
     1801 gtaaaagtag gtcatgactg aagtgccgtg atgctcctgg atgaaatcta taattgcatc
     1861 tggaagatta tactcctcgg ccaactccac accctctttc acatgtgatt ccaaaataag
     1921 agcgctcatc gtcggtgcca atttctcgtg tttgctttta gctcccatct gattctccac
     1981 aaaatattct ggtttcttta tcttgccgat atcgtggtaa taagagccaa cccttgccag
     2041 aagagtattg gcgccaatct ccttagcagc tgcttcgact aagtttccca atacgatgct
     2101 atgatgatac gttcccggag cttccagagt cagtcttttg agaatgggat gattcaaatc
     2161 ggagagctcc aaaagcgaaa tgtctgtggt tatgttgaaa agcgattcaa agatcggcaa
     2221 aaaccctata ctgagaagga tggaaaggaa accgtttgcc aaacctattc cacatagttc
     2281 tagtatctca ttggtagaag tgtgtctgag atattccata aagaagataa gaacaatata
     2341 ggcgaagctg atataaagca taggtcgata aaaacgataa cgtttctgta cctccttaac
     2401 actgtaacaa cccagggtcc caactacaat agtgaggaaa gtcaatttga aatcgaaatt
     2461 gtgcacaacc cctaaaagta gtcccaatgc cacggtggaa accagtccta cctccacatc
     2521 tataagtatg gttaaaagca atgaggcaat caccaccggg atagtgtact ggggccaacc
     2581 tagcttgaag gttaatacca aactcaagaa tatctctaag aggattatca gacatatcat
     2641 caaaagcttg ggattattgt tgaatatctt ccgtttgaag aagtaaagga aaagtcctaa
     2701 aaacaagaaa acgaaggtaa caaaaagagc tcgtcccaca taaggcagaa cagcttctgt
     2761 gcttctggac tcgtgctctg cataagccaa ggactcaagt ttttccagat gttccttggt
     2821 gactttctcg ttcttgccta ttatcctttc accttttaga accaaacctt tgtaagggga
     2881 aattaagctt gtttcttctt ttatctttcc cttagtttct tccacatcca ttttcaaatt
     2941 aggaattaag aattgggtgc tgatatcgta ggtcccctta gccaataatg gagcttcctt
     3001 gaacttggct gtggcagaag acaatatccc ctcctctgcc ttgactttgt ccaaaagctg
     3061 tttttgggtg aggatgtgct ccccattttt tctaaataca gccgcagtct ttactctatt
     3121 caaaggtaaa tcgtccgctt tcgccacaaa gccagaatcg tatatctcgg taagaactgt
     3181 agttaaaaat ctctttataa ccttaacgtt aggctcatga cttaaggcaa tttgggattc
     3241 ctggcttaga ttagggaatt gcagagcgaa ttgttgtaaa cgtaaagtag tgtcaggaat
     3301 agactgattt aagctatcca ccttagaaaa gaaacgcttt aactcaacca tcacggaatc
     3361 gaagacttta tcttgatatc tcaatatcaa aggttgatta gcgcgaatca tttgtcggtc
     3421 ctgctccatc tcctctggac ttttcagaat ctgaaaattg cgaggggaga tgatgtcctc
     3481 ataagcggct tcgccctcct gaggggtttg taatggtcga tagaccgaag aggacggata
     3541 tatgaaagtt actatcccga ttacaaccaa agccagcact actttgaaca aataatgctt
     3601 cctctggtta actctacctt tcttgtcatt atccgtcttt ctgaagacga acttttttat
     3661 ctttcttctt gtttttagga aaaattcaaa caccatttgc agtttaacct ctacttgctt
     3721 ttgcgacctc tatgttcata tttttcgtac gctcttataa tatcctgcac aagtttgtgt
     3781 ctcactacgt ccttttctgt aagatagaca aactttatcc cccctatccc ggacaaaatt
     3841 ttctggctct gcaccaaacc cgaggctttc ttgttttcta aatctatttg agttatgtct
     3901 ccggttacaa ctgcctttga atttgtgccc aaacgagtga gaaacatctt catctgggca
     3961 gtggtggtgt tctgggcttc atccaagata acaaaggagt tgtttaaggt tctaccgcgc
     4021 ataaaagcca agggcgcgat ttcaattacc cccatttcca taagttttct tattttctcc
     4081 ataactaaca tatcatgcag ggcatcatat actggtcgta aatacggatc aactttggct
     4141 cttatatccc ccggcaaaaa ccccaggttt tctccggctt caactgccgg cctaaccaaa
     4201 acgattctgc caactctttt tttcttcaaa gccgagactg cctcagccac agccagatag
     4261 gttttgccgg ttccagctgg acctatagat atgactatat cgtatttttc aattgcttca
     4321 acatattctt tttgtcctaa ggttcgtggc tttatctctt ccttggtcaa aggtgaagtc
     4381 ataagaccag cgatctgcat ttcattgaaa ggaccaactc cactctgcct gaccatactg
     4441 acggcatata agacatatct ttgagaaagc tctccgttct tcttgagatg ggaaatcagg
     4501 tctctaaata aaacgcaaag ctgttcaact tctttttcct ctccttcgag atggataaca
     4561 tccccgcgcg caactatctt agcatcaaac tggctttcga tgagatttaa aagtacacca
     4621 ttctggccaa agagcaacct ttggtcaaca cctgaaatat caatgctttt ggcggtattc
     4681 aatttttcca tcctttctag ggcaaacgca ttaaatgttg ttgcatatta gagcaatatg
     4741 gaatctattt taaaatcccc ctttgttccc cctttttcaa agggggagac ctcttctttt
     4801 cccctctttg taaaagaggg gttaggggag atttattgaa tgtaaattaa tgcgtttgta
     4861 ttagattaat tttattccca attccctcaa ttgcttttcc tccacttccg aaggtgaacc
     4921 atccatcaag gattgagccg cggttgtctt ggggaaagca atcacatccc gaatcgattc
     4981 agttccggtc atcaaagcca cgattctatc catccctaat gcaatcccac cttcaggcgg
     5041 tgcaccatat tccagagcat ccagaagaaa tccaaatctt ttttcagcct ggtttttatc
     5101 cattcctaat actttgagaa gtttttcctg tatctccctt ttgtgatttc ttatcccacc
     5161 agaagctatc tccacaccgt ttaaaactaa atcatactga ttagctttga tttttgccca
     5221 aggatgattt ctgtcgtcca aaggcaactt agaggaaaac ccctcatcca gttttggaat
     5281 gtcttcctcg tatggcatag tcacaatgtt atgcattgcc tgaaaagatt tctcctccgg
     5341 attatattcg aacaaaggaa actggtaaat ccattcaaag ctccatttgt ctttgggaat
     5401 cagattgtat tttctggcaa tttcagttct caatttcccc aagactggat agactatttc
     5461 gggcttatca gccacaatta aaatcaaatc tccgattttg gctttcattt tgtctattat
     5521 ggatttcatt tcttcctgac ttatatattt tagaatcggc gatttgaacc cctcacttga
     5581 aaaaacaatc cagcttagtc ctttggcttt ggattgttta gccatttctt cgagtttttc
     5641 taattctttt ctggaaagcg tgccacctcc attgaaaaca atgccacaca ctttcccgtt
     5701 accttttatg gtatcagaga agactttaaa ttgtgagttt tcgactatgt ccgataagtc
     5761 gcaaacctcc atttggtatc tcaaatccgg tttatcagtt ccaaacttgg tataagtttc
     5821 attgtaactt agtcgaggaa aaggaatttt cacttcaaca ttcaaaactt tcttgaaaat
     5881 atatgccatc attttctgga caacttcaaa gacatcatcc tgtgtgacaa aagacatttc
     5941 cacatctatc tgcgtatgct ctggctggcg gtctgctctc aaatcctcat cccgcagaca
     6001 tcgtgccatc tgaaaatatt tgtcaaagcc ggacatcatc aagatttgct taaaaagctg
     6061 cggtgattgg ggcagagcat agaatcttcc cttattcacc cggctgggaa cgatataatc
     6121 ccttgctcct tcgggcgtgc tacgaatcaa aagaggggtt tcgatttcat aaaaaccctg
     6181 ctcatccaaa taatccctga ccgcctttac ggctttat
//
LOCUS       DASWLN010000023        20059 bp    DNA     linear   ENV 20-FEB-2024
DEFINITION  MAG TPA_asm: Candidatus Zixiibacteriota bacterium isolate
            SMAG_U2949 k141_1153082, whole genome shotgun sequence.
ACCESSION   DASWLN010000023 DASWLN010000000
VERSION     DASWLN010000023.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37960594
            Sequence Read Archive: SRR10376091
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Candidatus Zixiibacteriota bacterium (soil metagenome)
  ORGANISM  Candidatus Zixiibacteriota bacterium
            Bacteria.
REFERENCE   1  (bases 1 to 20059)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 20059)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (26-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/12/2024 08:28:26
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 1,440
            CDSs (total)                      :: 1,416
            Genes (coding)                    :: 1,412
            CDSs (with protein)               :: 1,412
            Genes (RNA)                       :: 24
            rRNAs                             :: 1 (16S)
            partial rRNAs                     :: 1 (16S)
            tRNAs                             :: 21
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 4
            CDSs (without protein)            :: 4
            Pseudo Genes (ambiguous residues) :: 0 of 4
            Pseudo Genes (frameshifted)       :: 0 of 4
            Pseudo Genes (incomplete)         :: 3 of 4
            Pseudo Genes (internal stop)      :: 1 of 4
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..20059
                     /organism="Candidatus Zixiibacteriota bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_1153082"
                     /isolate="SMAG_U2949"
                     /isolation_source="soil"
                     /db_xref="taxon:2053527"
                     /environmental_sample
                     /country="USA: Oregon"
                     /lat_lon="42.99 N 123.63 W"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            <1..1647
                     /locus_tag="VGB16_00710"
     CDS             <1..1647
                     /locus_tag="VGB16_00710"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_018673925.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_function: GO:0016887 - ATP hydrolysis activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="AAA family ATPase"
                     /protein_id="HEX9916231.1"
                     /translation="FIDELHTIVGAGGAEGSLDASNIFKPALSRGDLQCIGATTLNEY
                     RKYIEKDGALERRFQTVMVDPPSLEDTIKILKGLKSKYEEHHKINISDEAMESAVKLS
                     DRYVSGRFQPDKAIDVIDEAGSKVHLSTCTKPETFGLLEEEISTLQKQKEQSVKNQEF
                     EKAAQLRDELKFKKEELEAKRKDWEGDRSKNVVTLTEEDIAEVVSKMTGIPLFKLEEK
                     ESKKLLRMEEELARRIVGQREAIAAIARAIRRSRAGLKDPRRPIGSFIFLGPTGVGKT
                     ELGRALASFLFEDEDSLVRIDMSEYMEKFAVSRLIGAPPGYVGYEEGGQLTEKVRRKP
                     YSVVLFDEIEKAHPEVFNILLQMLDDGQLTDSFGRKVDFRNTVVIMTSNLGTRLAKEG
                     KSLGFQKRDEESSYEQMKQKITDELKKTFNPELLNRIDETIIFHPLGMDDIIQIIDIL
                     SIDVAKRLADKGISFTLTKDAKKFLAEKGYDPTFGARPLKRALQKYLEDRLAEEILSG
                     QFAGDCNVLIDKKDEDSLSFSINPNQKSTLEEEKGSLTKK"
     gene            1717..1968
                     /gene="rpmA"
                     /locus_tag="VGB16_00715"
     CDS             1717..1968
                     /gene="rpmA"
                     /locus_tag="VGB16_00715"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_015718500.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0000315 - organellar large ribosomal
                     subunit [Evidence IEA];
                     GO_component: GO:0022625 - cytosolic large ribosomal
                     subunit [Evidence IEA];
                     GO_function: GO:0003735 - structural constituent of
                     ribosome [Evidence IEA];
                     GO_process: GO:0006412 - translation [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="50S ribosomal protein L27"
                     /protein_id="HEX9916232.1"
                     /translation="MAHKKGVGSSRNGRDSHGQRLGVKRFSGETVVGGSILVRQKGTK
                     FHPGYNVGMGKDNTLFSKVSGKVKFERKGAERKLVSVYI"
     gene            2042..2713
                     /gene="elbB"
                     /locus_tag="VGB16_00720"
     CDS             2042..2713
                     /gene="elbB"
                     /locus_tag="VGB16_00720"
                     /EC_number="4.2.1.-"
                     /inference="COORDINATES: protein motif:HMM:NF008747.0"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="isoprenoid biosynthesis glyoxalase ElbB"
                     /protein_id="HEX9916233.1"
                     /translation="MKKIGIILSGCGFQDGSDPRESVFILLQLEKRSCQVQFASVKAI
                     QREVIDHHTQEIIPQSRDILVESARLSGINIQEIKDLSGAELDALIIPGGQGVIKNLS
                     SATENEKLLKTHPDLRKLLREMHRRKKPIGGCGVASLIIAESLGDICETSLTLTLGND
                     PQLIQQLEKMEAVHVIAKGDEAIMDEQNRIVTTPGAQLKQRTMDFYGGIENLIEGILE
                     LTSQP"
     gene            2733..3575
                     /locus_tag="VGB16_00725"
     CDS             2733..3575
                     /locus_tag="VGB16_00725"
                     /EC_number="4.2.1.2"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_008518494.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016829 - lyase activity [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="fumarate hydratase"
                     /protein_id="HEX9916234.1"
                     /translation="MRQIQVKDITKAVRDLCIDANYNIGQDVIDALKKSLKTEESPTG
                     KEIIEQILENDKIAQEEQVPMCQDTGFAVFFVDMGDQVCIKDGNLFKAINEGVKQGYT
                     EGYLRKSILDDPIKGKNTGDNTPAVIHLNIVSGDKLKISFAPKGGGSENMSEIKMMKP
                     SDGIEGIKKFVVDMVKRSGSNPCPPIVVGVGIGGTFEMCAQMAKKSLFRPIGSRNPDP
                     FYAKVEEELLEEINKSGIGPQGLGGRTTALDVHIEVFPRHIASFPAAVNINCHAARHK
                     EAIL"
     gene            3591..4148
                     /locus_tag="VGB16_00730"
     CDS             3591..4148
                     /locus_tag="VGB16_00730"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_014187446.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016836 - hydro-lyase activity [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="Fe-S-containing hydro-lyase"
                     /protein_id="HEX9916235.1"
                     /translation="MAEGKKITTPLTDEVVKSLKIGDKILISGPIYTGRDQAHKKMVD
                     LLEKGEKLPFDVKGQLIYYVGPSPAKPGKVIGSAGPTTSGRMNKYAPILLKAGSKGMI
                     GKGEMGGEVPEALKKHTGVYLATVGGAAALVAKSVVANELVAWPELGAEAVSKLTVKD
                     FPAIVVQDSYGGNLYVEGVKKYQRA"
     gene            4232..5473
                     /locus_tag="VGB16_00735"
     CDS             4232..5473
                     /locus_tag="VGB16_00735"
                     /inference="COORDINATES: protein motif:HMM:NF017760.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0022857 - transmembrane transporter
                     activity [Evidence IEA];
                     GO_process: GO:0055085 - transmembrane transport [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="MFS transporter"
                     /protein_id="HEX9916236.1"
                     /translation="MNKQTPEAATDRELSPHLFRNRNFVLLWIGRTISVLGDNIYAIA
                     LMWWVLEKTNSTAMMATVAICWTVPAVALGIIAGTYADRVNRKKLIIWMDLGRACLIA
                     IPATLLFFGLLEVWHIFIITILESSMSTFFGPSISAFLPSLVRKEDLTRANSMFQLSF
                     NLSGILGQALGGILMAVVGAAMTIYLDAISFFVSALSIISISTEITAPQTSIERKDFL
                     RDLKDGLIYIRQNQFIWGIISLAVIINFLFAPFGIYMPVMVKSVFKMGPQGFGYLGAA
                     VSLGMLAGSLFLSIKGQIKRKGLVIVGSVCLMGLCISFFGITQYFILSLLLLALMGVF
                     MAFANTLIVVVFQSQVPTGVQGRVFGTTSTFAQGLQPISLALAGVLSDLFKVQFIILT
                     SGILAFLVGIYGFFIKGIRDA"
     gene            complement(5573..5953)
                     /locus_tag="VGB16_00740"
     CDS             complement(5573..5953)
                     /locus_tag="VGB16_00740"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEX9916237.1"
                     /translation="MDALWNIVAVIAIAFLAGLWLKDRLSMSRVCRQAEAMVSEGNWR
                     EAARIYKANIAARLDSPRAMRELKRRLSLVYDANGITVNLDRLDQKPVEIKRILKSSR
                     NDKEKGRLVQQLIDDTKRWLAELP"
     gene            complement(6292..6675)
                     /locus_tag="VGB16_00745"
     CDS             complement(6292..6675)
                     /locus_tag="VGB16_00745"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEX9916238.1"
                     /translation="MSFNDEHAIETYKSLISISTEALKAFQWLNGGAVVALLAYIGQR
                     PELAIPARCALTLFVVGLIAASAAFVTSYLTQLALYNEDIREEKYQGTSHVIWLWATL
                     FIAIVSLACFSLGAFAGVRAFAQIP"
     gene            complement(6878..7600)
                     /locus_tag="VGB16_00750"
     CDS             complement(6878..7600)
                     /locus_tag="VGB16_00750"
                     /inference="COORDINATES: protein motif:HMM:NF019284.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="toxin-antitoxin system YwqK family antitoxin"
                     /protein_id="HEX9916239.1"
                     /translation="MKNILSLLTNLVGFQRGVMVINLLSCLFAVLLLIGLNQVALSQV
                     ALEEKIQCPEGTKSMDGKNDGYTAKWCQKANGAYHGPMITWYDNGQKRSEKNYLDNST
                     HGRSIMWYENGKKELETNYTNDKLEGLYTAWYENGKKQFEGNFHNDLKQGHWTMWYEG
                     GAKSKDIEFQDDKRHGIEIEWYESGVKKSETPYQHAIQIGKWTEWHPNGKKYREGETR
                     LENGQLIVLDTKEWDEKGQLVK"
     gene            complement(7728..8471)
                     /locus_tag="VGB16_00755"
     CDS             complement(7728..8471)
                     /locus_tag="VGB16_00755"
                     /inference="COORDINATES: protein motif:HMM:NF015724.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0030288 - outer membrane-bounded
                     periplasmic space [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="CsgG/HfaB family protein"
                     /protein_id="HEX9916240.1"
                     /translation="MKTRVLFGLMGLVVVSTFLAGCVSTQPTAKVDATAGAAPLAPYN
                     GPKVSIAVADFEVKAAKAGGDIGSGLRDMLTTALVQSNRFKVLERQALAAVQQEQELA
                     ASGAADKTTGPKKGKIKTADIIVTAAVTEFEPQASGIKAGGGAGAGIGGAIVGGILGA
                     AKNKAHMAMDIRIIDATTSEVLTATRVEGEAKDVAGGFGAGFFGGGVLMGAGLSAYAN
                     TPMEKAIRVCINEAVRYLSETTPKEYFKY"
     gene            complement(8556..9842)
                     /locus_tag="VGB16_00760"
     CDS             complement(8556..9842)
                     /locus_tag="VGB16_00760"
                     /inference="COORDINATES: protein motif:HMM:NF015724.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0030288 - outer membrane-bounded
                     periplasmic space [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="CsgG/HfaB family protein"
                     /protein_id="HEX9916241.1"
                     /translation="MNKSVSKLELLIVLAICLFLCLVGCAGLTHYGKLEKNARQNYAD
                     ENYDQAVYSCANSLRINPRYEKSQELIQVAFTAAISLHENKIKELEFSSAKFKWDEIV
                     FHYNSLVKLNQTVAALPKLKAKKTNREIKFDLKNYTSELVESRKNAAEEHYQEGIRLS
                     NDGNVDVQRSAAKEFKTAEMFVPGYKDAGSQYLKSHRAALKRIAIIPFENKSGKDWYG
                     ALSELITDEITSDIMNDSSVMEFLEIVSRDQLEEIMREQDLGLSGIIDEQTAVELGKV
                     LSVHEIVTGQITQIISAPERTTRSKGKQTARVVIGTEKYKDGEGKTRERNVYGDVHAD
                     VTYYTKAASASISGSYRIIDVRTAEVKKSQSITGKYEFKHKYARFSGDERALSSDAHT
                     LVLKDEGLAPVDEEMVNYAAKDLSALLANTLKQYAK"
     gene            complement(9891..10580)
                     /locus_tag="VGB16_00765"
     CDS             complement(9891..10580)
                     /locus_tag="VGB16_00765"
                     /EC_number="1.3.5.1"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_013007413.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008177 - succinate dehydrogenase
                     (ubiquinone) activity [Evidence IEA];
                     GO_function: GO:0046872 - metal ion binding [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="succinate dehydrogenase iron-sulfur subunit"
                     /protein_id="HEX9916242.1"
                     /translation="MVQFRVFRYDPDKGGKPHFQSYKFEEEKGMTILEGLFHIFENID
                     GSLAFRSSCREAVCGSCAMHVNGMYRLACKTQIGVLGSRITIRPLAHLPVIKDLITDL
                     TPFFEHLKEIKPYLIPKEVPPQKEYIQSVEQRRKIDEWLDCILCGACYGSCPVASQDK
                     KYLGPHALLKALRFVEDSRDGGAKERIAVVAKDTGIFRCHTIFNCQKVCPKDLDPTKA
                     IAKLKMKTLFG"
     gene            complement(10599..12299)
                     /locus_tag="VGB16_00770"
     CDS             complement(10599..12299)
                     /locus_tag="VGB16_00770"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_012458982.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="FAD-binding protein"
                     /protein_id="HEX9916243.1"
                     /translation="MIFHDILIVGGGLAGLRAGIAAAEKGVDVAIISKIHPMRSHSGE
                     AQGGINAALANVEGSKDDTPEKHLFDTVKGSDFLADQDASEVLTSSGPEKIFEMEHWG
                     CPFSRTEAGRIAQRPFGGAGFSRTCYATDKTGLYLLQTTWEQNIKHNVKVYEEWIVLG
                     LATENGKCCGVIAMELLSGELHAMGANAVIFATGGIGRLYANTTNAITNTGLAMAIPY
                     WAGVPLKDIEFMQFHPTALARTHILMTEGCRGEGGYLKNRLGERFMSKYVSEKVMELG
                     PRDIVARSIQTEINEGRGFPDGTVHLDLTHLGAAKIMERLPGIRDICLNFVGIDPIKE
                     PIPITPGTHYEMGGIDTDKDGQTPMEGFLAAGECACVSVHGANRLGGNSLLETIVFGE
                     RAGKKAAEWVKGKDKKPKEKALGDCLKKQREALENCKKGGGTENPYKIRDELANLMVK
                     CFGLFRKEEDMKAGLEGIRKLKDKFKKIRPAYSGKAYNMDLISCYEVGSNLDVAEALA
                     VSALARQETRGSHYRTDFNKRDDVNWLKHTLTTHAPDGPKLSYRPVVITRFKPEERKY
                     "
     gene            complement(12319..12717)
                     /locus_tag="VGB16_00775"
     CDS             complement(12319..12717)
                     /locus_tag="VGB16_00775"
                     /inference="COORDINATES: protein motif:HMM:NF013307.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEX9916244.1"
                     /translation="MYEQKSGAIKYYLDEWSLNANIGTFSWLLQRLTGIVLTLYLFMH
                     ILAVSSGLWGSETFNIWLKNLQTPLTHFLEIFLLAAVAIHMLNGLRVTIADFFFVTRQ
                     HKLMFWLGTVLFALFMVATLMVLLPLAFGH"
     gene            complement(12732..13085)
                     /locus_tag="VGB16_00780"
     CDS             complement(12732..13085)
                     /locus_tag="VGB16_00780"
                     /inference="COORDINATES: protein motif:HMM:NF013307.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEX9916245.1"
                     /translation="MSQDLQRGTATGWLWQRITGILLAFFVVVHLNVLHFSQRQFIDF
                     NVVSQRLGSSSWWVIFYLVFIVVVLFHGLNGAWQIIHDYRPSPGAQKAIKAFLWILGV
                     VATVYAIYAITPFRR"
     gene            complement(13452..14864)
                     /locus_tag="VGB16_00785"
     CDS             complement(13452..14864)
                     /locus_tag="VGB16_00785"
                     /inference="COORDINATES: protein motif:HMM:NF017044.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0046872 - metal ion binding [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="pitrilysin family protein"
                     /protein_id="HEX9916246.1"
                     /translation="MYKNMFKTALALVILAFCFSYLASISLLSAETSDEIKLPQIQTK
                     VLENGMKVMVIEQHNLPVVAFRLVLKTGAAYDPKDKAGLANLTAGLLTKGTTTRTAPQ
                     IAQEIDFVGGSLGAGANLDASNATCRVMSKYFDTGLDLLSDIVLNPTFSESEVKRLKR
                     QTLAAIIQKKDDPNTIMEEKFKQFLFGDHPYARPVSGDDKSVAAITPDDIVEFHKTFY
                     VPNNAVLAVVGDVKAEDVIKKIEAKFGNWQKKSLPVVKFASPPEVKGYDILLVDKPDL
                     AQAYISIGHLGLARKDPDEFPSKVMNYILGGGGFSSRLMAKVRVEKGLTYGVYSSFNL
                     NRDPGSFDVSMSTQVDSGLAAIGAILEELKKIREAEVTKKELDDTKSFYTGYYPLRFE
                     TPEQVATQVLDTEIYGTGLDYIKNYRKNIKQVTVAEVLTAAKQYIHPDDLKMVVVANA
                     EKVKAGLEKLGTVKVISFTE"
     gene            complement(14880..16229)
                     /locus_tag="VGB16_00790"
     CDS             complement(14880..16229)
                     /locus_tag="VGB16_00790"
                     /inference="COORDINATES: protein motif:HMM:NF017044.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0046872 - metal ion binding [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="pitrilysin family protein"
                     /protein_id="HEX9916247.1"
                     /translation="MFKTLNRLALSLLLLLFLTQIGSAQVHFDVQKFTLDNGLTLLVA
                     EDHSAPVASFSIWYKVGSKYERPGITGISHMCEHMMFKGTRKVRPEEFSKIVQANGGI
                     VNAWTSFDNTTYWEKVSGDKLELVMSLEADRLKNLKLANEDFQPERNVIAEERRQTLD
                     DQPFDAVFEQLLNHTFVAHPYHWIVIGFMSDIQSWTFDQLKNHFKTYYVPNNAVAVVA
                     GDIKAEDALKLTKKYFGKIKAGPAPTKVTEIEPEQLGERRIEVHKIAQLPGLAVSYKV
                     PKLGDPDSYPFEVAAKILFSGQSSRVYKKMVYDEQMALFVDGGAWILQDAGVFYAFVG
                     MQPGRDLKEGEKALFAEIDRLCTEPVSEKELQKAKNQLESEFVFGLQGADNKGFQIGH
                     YEAIYGDYKLLFEIGEKYQAVTVEDVMRVAKKYLDPRKRTIALLIPEAPPTAMTEQR"
     gene            complement(16503..17300)
                     /gene="rlmB"
                     /locus_tag="VGB16_00795"
     CDS             complement(16503..17300)
                     /gene="rlmB"
                     /locus_tag="VGB16_00795"
                     /inference="COORDINATES: protein motif:HMM:TIGR00186.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008173 - RNA methyltransferase activity
                     [Evidence IEA];
                     GO_process: GO:0009451 - RNA modification [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="23S rRNA
                     (guanosine(2251)-2'-O)-methyltransferase RlmB"
                     /protein_id="HEX9916248.1"
                     /translation="MFSQRKSKEILKLKNKKFRQKQGKFMVEGVRSVEELLSSDWEVE
                     VLLYSSLLEKSDRANFILRKATSRKIAIEKISQDILDKISEAVSSSGIVCVVRTRDLK
                     LDDLLKGNPQSILVLDEVGDPGNLGTLIRTADAAGINGVILSEKTVELYSPKVVRSTM
                     GSIFHLRTAEKTDLVKSLRRLKEEGFKIIASAVKGGEPYHKIDYSGRVCLVVGNEIKG
                     VRAEISKLADKIANIPIYGKAESLNASVAGGILMYQMAIFKLTETSA"
     gene            17611..>20059
                     /locus_tag="VGB16_00800"
     CDS             17611..>20059
                     /locus_tag="VGB16_00800"
                     /inference="COORDINATES: protein motif:HMM:NF013283.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_process: GO:0006629 - lipid metabolic process [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="patatin-like phospholipase family protein"
                     /protein_id="HEX9916249.1"
                     /translation="MKRKTIIFLLTSLLFSSVWAEGKTIRLEFGEPNSQSINLPLKKQ
                     LKRKKVGLALSGGGARGFTQIGILKVLEREQIPIDYIAGTSMGGVVGGLFAAGYSAEE
                     LEELVLKTNWEDILRDNPNRLSLFLSQREESEGYFLKIRFNGFRPYMPTALTSGQKIT
                     DLFTSLTAGANYRARANFDYLKIPFRAVATDLETGQKVVLGSGDLAEALRATISVPLA
                     FAPVELDQKVLVDGGLVEPIPVETAKEMGAGIIIAVNTSTSLLSWKKITNPIDIADQT
                     TSIMSLDEKERQLSQADLVISPELGGYSAVDFDKAKELIAIGERACDQVVSRIKQIVS
                     EKDTLGQETYYLTEVEFDGAEQINKDTLERICPIQAGDSITLKSIEDYLREIYGSGYF
                     SDAYAELEPYRSGYVLVFNLKENQILREILFSGNSIFSTSQLKKELSLELGQTLNSQR
                     LERGLDNVLELYKQKGHALASISKVNFNSGALLIEINEGIISEIRVEGNHRTKNWVIL
                     RSFPLKVGKPFNSNLADKGVEDIYATELFDKVTLKILPGEKGAILDISIKEKDFTFLR
                     IGMHYDDEHNLESALQLMDANILGIGNKTYADLLYGNTKQRYGLHFKADRIYKSYLTY
                     RGDIFHQREKKKIYSEHREIDSFREWRTGTVFSLGQHISRLGTLSLETKLERVELREG
                     KIYPKEVNDVRSITLRSLVDTFDKYPFPDRGKYHHLYIELAGRILKGDLTFRKGFTSL
                     ESYFPLSKHWNFHPKIALGISDGNLPTSEQFLLGGYNEFYGFFQDELRGDKLIYGNLE
                     MRFKFWQRFYWNVRYDIG"
BASE COUNT         5504 a         4569 c         4302 g         5684 t
ORIGIN      
        1 ttcatagatg agctgcatac cattgtagga gcaggtgggg ctgagggctc tctggatgct
       61 tccaatatct tcaagcccgc cctctccaga ggagacctac agtgtatcgg agcaaccact
      121 ttaaatgaat atagaaaata tatcgaaaag gatggtgccc tggagagacg ctttcagaca
      181 gtaatggttg acccgccttc cttggaggat accataaaga tattgaaggg acttaagagc
      241 aaatatgagg aacatcataa aataaacata tcggatgagg ctatggaatc tgcggttaaa
      301 ctctcggata gatacgtctc cggtaggttt caacctgata aagcaattga cgtaatcgac
      361 gaagctggtt ctaaagttca cctatctact tgcactaagc cggagacctt cggacttctt
      421 gaagaggaga tatccacact gcagaaacaa aaagagcagt cagtaaagaa tcaagaattt
      481 gagaaagcgg ctcagctcag agatgaactg aaattcaaaa aagaagagtt ggaagctaaa
      541 cgcaaggatt gggaaggcga ccgttccaag aatgttgtga ctttaaccga ggaggatatt
      601 gctgaagttg tttccaagat gaccggcata cctttgttta aactggagga aaaagaatcc
      661 aaaaaactcc tgcgcatgga agaggaatta gccagaagga tagtgggaca acgagaagct
      721 attgctgcca ttgcccgagc catccgcaga agcagggcag gactgaaaga cccacgtagg
      781 cccatcggct ccttcatatt tctgggacca accggagtgg gcaagactga actgggcaga
      841 gccctggctt catttttgtt cgaagatgag gattccttgg tgagaattga tatgtctgaa
      901 tatatggaga agtttgcggt ttcgagacta atcggtgctc ctccaggata cgtgggatat
      961 gaggagggcg ggcaactgac tgaaaaggtg aggagaaaac catactctgt cgtgcttttc
     1021 gatgaaatag aaaaagctca tcccgaagtt tttaatatac tattgcagat gctggatgat
     1081 gggcaattga ccgactcgtt tggaagaaag gtcgatttca gaaataccgt ggtgataatg
     1141 acttctaatt tgggaacacg tttagccaag gaaggaaaat cactcggttt ccagaaaagg
     1201 gatgaagagt cctcgtatga acagatgaag caaaagataa ccgatgagct caaaaagacc
     1261 tttaaccctg aactccttaa ccgcatcgat gaaaccataa ttttccatcc tctcggaatg
     1321 gacgatatca tacagataat tgatattcta tcgattgatg tggctaagag gttggcagat
     1381 aaaggaattt ccttcacctt gaccaaggac gccaagaagt ttctggcaga aaaaggctac
     1441 gatccaacct ttggtgccag gcctttgaaa agagccctcc agaaatacct ggaggatcga
     1501 ttggcagaag agattttgag cgggcagttc gccggggact gcaacgtctt aattgacaag
     1561 aaagacgaag atagtcttag cttctccata aacccgaacc agaaatccac actcgaagag
     1621 gagaaaggtt ctctcaccaa gaaataaagc ttgtttttgt ggagtaattt gttatattat
     1681 tgcgattcat aattctaact tagaaggtga ttaaatatgg cacataagaa aggtgtcggc
     1741 agctcgcgta acggcagaga ttcacatggc caaaggttgg gagtaaagcg cttctccgga
     1801 gagaccgtag tgggaggtag catactggtt cgacaaaagg gaaccaagtt tcatccggga
     1861 tataacgtgg gaatggggaa agataatacc cttttctcta aggttagcgg gaaggtgaag
     1921 tttgagagaa agggagctga aagaaaatta gtgagcgttt acatttaagc catctttcga
     1981 atccgaccct cttcattgaa tctccaactg cactgaacat aagctggttc tgctctttaa
     2041 tatgaaaaaa atcgggatca ttttaagtgg atgcggtttt caggatggct cagacccgag
     2101 agaatctgtc ttcatcctcc tgcaattaga gaagagaagc tgtcaagtcc aattcgcctc
     2161 agttaaagct attcaaagag aagtgataga ccatcatacc caggaaatta ttccccaatc
     2221 aagagatatc ctagttgaat ccgctcgcct ttcaggaata aatatacaag agataaaaga
     2281 cctttccggc gcggagctgg acgctttaat tatccctggc gggcagggag taatcaagaa
     2341 cctgtcttct gcgactgaga atgagaagtt actcaaaacg catccggatt taagaaaact
     2401 cctacgcgag atgcacagga gaaaaaaacc tatcggagga tgcggggtgg ccagtctaat
     2461 cattgcggaa agcttaggcg atatctgtga aacttccttg actctgactt taggaaatga
     2521 cccccagcta attcaacaat tggaaaaaat ggaggcggtg cacgttatcg ctaaagggga
     2581 tgaggcaatt atggatgagc aaaatagaat tgtaaccacc cccggcgccc agcttaaaca
     2641 gaggacaatg gatttttatg gtggaattga gaacttgatt gaagggatat tggaattaac
     2701 ttcacaacct taactaaaga ggaggtactg ggatgcgcca gattcaagtc aaagatatca
     2761 ccaaggcggt cagggatctg tgcatcgatg ccaattacaa tattggccaa gatgtaatag
     2821 atgccttaaa aaaatctttg aaaacagagg aatctccgac cggcaaagaa attatagaac
     2881 agatattgga gaacgacaag atcgcccagg aagagcaggt gccaatgtgt caggatacgg
     2941 ggtttgcagt atttttcgtg gatatgggtg accaggtttg tattaaagat ggaaatctgt
     3001 ttaaagctat aaatgaggga gtgaagcagg gatatactga aggatatttg aggaaatcaa
     3061 ttctggatga tccaattaag ggcaaaaaca ccggggacaa cacgccagcg gtgattcacc
     3121 tgaatattgt gtcgggggat aaactgaaga tatcttttgc ccccaagggt ggcggaagcg
     3181 agaatatgag cgagatcaaa atgatgaaac cgtcggatgg aatagagggc atcaagaaat
     3241 tcgtggtgga tatggttaaa aggtcgggaa gcaacccctg tcctccgatt gtggtaggag
     3301 ttgggattgg cggaacattt gagatgtgcg cccagatggc taagaaatct ttgtttaggc
     3361 ctattggctc tcgcaatcca gacccattct atgcgaaagt tgaagaggag ctcctggagg
     3421 agataaacaa atcgggaatc ggtccgcagg gcttgggtgg caggacaact gccttggacg
     3481 tgcatatcga ggtttttccg agacatatcg ccagctttcc ggctgcggtt aatatcaatt
     3541 gtcatgcagc tcgacacaaa gaagcgatct tataaaaatt ggaggtaaaa atggctgaag
     3601 gaaaaaagat aaccacacct ttgacagacg aggtggtaaa aagcttgaaa atcggggata
     3661 agatattgat ttcgggtccc atctacaccg gaagagacca agcccacaag aaaatggttg
     3721 accttttaga aaaaggagaa aagctaccat ttgatgttaa ggggcaatta atttactatg
     3781 tgggtccatc acctgccaag ccaggaaaag tcatcggctc tgctggacca accacttccg
     3841 gcagaatgaa caaatatgct cccatacttc tgaaggctgg ctctaaaggt atgatcggta
     3901 aaggtgagat gggaggagaa gttccggagg ctttgaaaaa acatactgga gtttatctgg
     3961 ctacagtggg aggagcggca gctttggttg ctaagtcagt agtggcaaat gagcttgtgg
     4021 cttggccaga attgggagcg gaagcggtta gcaaactaac tgtaaaagat ttccctgcca
     4081 ttgtagtgca ggattcttat ggcgggaatc tttatgtgga aggagttaag aaataccaga
     4141 gagcttaaat ttaccaggaa aatataagaa ttgaatgggt gttaatatag ggcaggtatc
     4201 tttgtacctg ccctatcaat ttgagaatcg aatgaacaaa caaacgcctg aagctgctac
     4261 tgatagggag ttatcccctc atctttttcg caaccgtaat tttgtcttac tctggatagg
     4321 acgcaccatc tccgttttgg gtgataatat ttatgcaatc gctttgatgt ggtgggttct
     4381 ggaaaagacc aattccaccg ctatgatggc aaccgtcgcc atctgttgga ctgtacctgc
     4441 agtagctttg ggtattatag ccggaacgta cgctgacaga gtcaatcgca aaaaacttat
     4501 tatttggatg gatttggggc gagcttgcct tattgccatt cccgccactt tacttttttt
     4561 cgggctgctg gaagtctggc acatatttat tatcaccatt ctggagtcaa gcatgagcac
     4621 tttcttcgga ccaagcatat ctgcgtttct cccctccttg gttagaaaag aggatttgac
     4681 cagagccaat tcgatgtttc agttatcgtt taatctgagt ggtattctgg gacaggcatt
     4741 aggaggtatc ttgatggccg tagtgggtgc tgctatgacc atatacctgg atgccataag
     4801 tttttttgta tctgccttgt caataatttc aataagcacc gaaatcacag ctcctcaaac
     4861 ctcaattgaa agaaaggatt tcctgcgcga tttgaaagat ggcttaatct acatcaggca
     4921 aaatcaattc atctggggaa taatttcctt agccgtgatc ataaatttcc ttttcgctcc
     4981 tttcgggata tacatgccag ttatggtgaa gagtgttttc aagatgggac cgcagggatt
     5041 cggatactta ggcgcggctg tgtctctggg gatgcttgcg ggaagtctct ttttgagcat
     5101 caaaggccaa ataaaaagaa aaggattggt gattgtaggt tctgtttgtc tgatgggact
     5161 atgcatctcc ttctttggca taacccaata tttcatatta tctctgttgc tccttgcttt
     5221 gatgggagta tttatggcct ttgccaatac ccttatagtt gtggtattcc aatctcaggt
     5281 gccgactggg gtgcagggaa gggtctttgg aacgaccagc accttcgctc aaggacttca
     5341 acccatctcc ttagctttgg ctggtgtgtt atccgattta ttcaaagtgc aatttataat
     5401 tttgaccagt ggaatcttag cttttctggt tggcatatat gggtttttca taaaaggcat
     5461 aagagatgct tgagcatatt tctgcttgaa atgaaaaagc cccgtggctt tacgccgcag
     5521 ggcttagtaa tattataaac ggtccttaat actcttgggt tatgtcagga tctcacggca
     5581 gctcggccag ccagcgtttc gtgtcatcga tcagctgctg gacaagcctg cccttctcct
     5641 tgtcattcct actcgacttc aggatgcgct tgatctcgac gggcttctgg tccagacggt
     5701 ccagattcac cgtgatccca ttcgcatcgt atacaaggct caatcgtcgt ttgagttctc
     5761 gcatagccct tggagagtcg agacgtgcgg cgatattagc cttgtagatt cgtgccgcct
     5821 ctctccaatt cccctctgag accatcgcct cggcctgccg acagaccctg ctcatactga
     5881 gtctgtcttt gagccatagg cctgcgagaa aggcgatggc aatgaccgcc acaatattcc
     5941 agagtgcatc cacgcgctct cacctcctga ggtccagcga atctggccga tcgcggacgc
     6001 tgcgtttgac gtcgcgaggc aacctaaact tgcactgccc aggctgcggt ctaacaatgc
     6061 ttatacggtc tgtataatac gggaaacgca gactttttgg gttatgtcag gagtagctcc
     6121 tgacgcaacc ccagaatttc aggttctcta tctgctcctc agtgttgtaa gtgcgagatg
     6181 tttttctcgt cctctatcct ttcctgaacg ccgggcgtca acgatagaac tcagccgacg
     6241 gcgtggccgg tcggctggag tgatttgttg ggccggttgc catgattttg ttcaaggtat
     6301 ctgggcgaac gcacgaactc ctgcaaacgc tcctaagcta aagcaagcaa gactaacaat
     6361 agcgatgaaa agtgttgccc agagccagat aacgtggctt gtgccttggt atttctcttc
     6421 gcggatgtct tcattataaa gagcaagctg ggtgagatat gaggtgacaa atgctgcgct
     6481 tgcggcaatg agaccgacca cgaacaacgt gagggcgcac ctcgcaggaa tagctaactc
     6541 agggcgctgc ccaatatacg ctaaaagggc aaccacagct ccaccattca gccattgaaa
     6601 ggccttcaat gcctcggtgg agatggaaat gagagacttg taggtctcga tcgcgtgttc
     6661 gtcgttgaaa ctcataatct agatgccgcc taacgaccag cataacctga tgcaaggtat
     6721 aaagcacgac atgttttgaa tgaagaaaac ctaaatttaa tatctggcat ttttaaaatt
     6781 actgatccct tgcattcagg ttaatgcaat ggttaggtgc ctatttttgt taaatatatc
     6841 taaaatctga ggtataaatg ttatttggct cgtgctgtca ctttactagt tgtcctttct
     6901 catcccattc cttcgtatca agaactatca gttggccatt ttccagacga gtttcccctt
     6961 ctctatattt tttaccgttg gggtgccatt ctgtccactt accaatttgg atagcgtgct
     7021 ggtaaggcgt ttctgatttc ttcacgccag attcgtacca ctcaatttca ataccatgtc
     7081 gtttatcgtc ttggaattca atatcctttg attttgctcc gccttcatac cacatggtcc
     7141 aatgaccttg ttttaaatca ttatgaaaat ttccctcaaa ttgtttttta ccattttcat
     7201 accatgcggt atatagtccc tccagtttgt catttgtgta atttgtttcc agctcctttt
     7261 tcccattttc gtaccacatt atcgaacgcc catgtgttga attgtcgaga taattttttt
     7321 cacttctctt ttgtccatta tcgtaccagg taatcatagg tccgtgatac gctccgttcg
     7381 ctttctggca ccactttgca gtatagccat cgttctttcc gtccatgctt ttagttccct
     7441 ctggacattg gattttctct tccaaagcta cttgactcag ggctacttgg ttcaaaccaa
     7501 ttagtaacaa aaccgcaaat agacaactca gcaaattaat aaccatcact cccctttgaa
     7561 atccaacgag gtttgtgagc aaactaagaa tatttttcat tatttatctc atttctttgt
     7621 tcgccgccga acattgttta tttgtatcca cataacacct caaacagtag ggacagaaca
     7681 gtgttctgtc cctactgttc ttttattgtg cttaccaata aaaattatca atacttgaag
     7741 tattccttcg gtgttgtttc ggatagatac cgcaccgcct cgtttatgca aacccttatg
     7801 gctttttcca taggcgtatt agcataagca gatagtccgg ctcccattaa cacaccacca
     7861 ccaaagaacc cggccccaaa accacctgcc acatccttgg cttcaccttc gactctggtt
     7921 gcggttaaga cttctgatgt ggttgcatct atgatcctta tgtccattgc catatgagct
     7981 ttattcttgg ctgcgcctag aatgccacca actatggctc cacctatacc agcacctgcc
     8041 ccacctccgg ctttaatgcc agatgcctgc ggctcgaatt cggtgaccgc agcggtaacg
     8101 ataatatcag ccgtcttgat tttccctttc ttaggtcctg tggtcttatc tgctgctccg
     8161 cttgcagcca gctcctgttc ctgctggaca gccgctaagg cttgacgctc aagcaccttg
     8221 aagcgattgc tctgcactaa agcagtggta agcatgtctc ttaaccctga gccaatatcc
     8281 ccacctgctt tcgccgcttt cacctcaaaa tcagccacag cgatgctgac ttttggccca
     8341 ttataaggtg caagaggggc ggctccggca gtggcatcta ccttggctgt gggctgggtt
     8401 gatacgcagc cggcaagaaa tgtgcttaca acaaccaaac ccatcaaccc aaacaaaacc
     8461 ctagtcttca tagctaaaac ctcctttcaa taaagtttgt ttttactttg cgttacattt
     8521 tagaatgtta gaatttcaaa aatcagtttg tttcactatt ttgcgtattg ttttagggta
     8581 ttggctaata atgcgcttaa atcctttgcc gcgtaattta ccatctcctc gtcaaccgga
     8641 gcaagccctt catcctttaa aaccagtgta tgagcatcgc tgcttaacgc cctttcatcg
     8701 ccggagaatc ttgcatactt atgtttaaat tcatatttcc ctgtaataga ctggcttttc
     8761 ttgacctccg ctgttcttac atcaataatc ctgtatgaac ccgaaataga agcgctggca
     8821 gcctttgtat aataagtcac atcagcatga acatcgccat aaacatttct ctctcgggtt
     8881 tttccttcgc catctttgta tttctcggtt cctatgacaa cccgggctgt ctgtttgcct
     8941 ttgctcctgg tagttctttc tggtgcagaa attatttggg ttatctgacc tgtgactatt
     9001 tcgtgcacac tcaatacctt gcccaactca acagctgtct gttcatcaat tataccggat
     9061 aatcctaagt cttgctcccg cataatctct tccagttggt ctctcgaaac aatctccaaa
     9121 aattccataa cgcttgagtc attcattatg tcgctggtta tttcatctgt aatcaattca
     9181 gaaagcgcac cataccagtc cttgccactc ttgttttcaa agggaatgat agcaattctt
     9241 tttagtgccg ccctatgaga tttcaaatat tgactgcctg catccttata tccgggcaca
     9301 aacatttcag ctgttttgaa ttctttggcc gcacttctct ggacatcaac attgccgtcg
     9361 ttgcttaggc gaatgccttc ctgataatgc tcctctgctg cattctttct tgattccacc
     9421 aactccgagg tataattctt taaatcaaat ttgatctctc tatttgtttt cttggccttt
     9481 aattttggca acgctgccac ggtttgatta agcttaacca atgaattgta gtgaaataca
     9541 atttcatccc acttaaattt tgctgaagaa aattccaatt cctttatttt attttcatgt
     9601 aaagagattg ctgctgtgaa agctacttga ataagctcct gagacttttc atagcgggga
     9661 ttaatcctta atgaattggc acagctgtag actgcctgat cgtaattttc gtcagcatag
     9721 ttttggcgtg catttttctc cagctttcca taatgagtta atccagcaca acctaccaga
     9781 cacaaaaata gacagatagc caaaacaatc aagagctcca gcttgctaac tgacttattc
     9841 attcaagccc ctccaggcaa gttttgtaat acttgcatat ctaaatattt ttaaccaaac
     9901 aacgttttca tcttcagttt ggcgatggcc ttggtcgggt ccaaatcttt gggacagacc
     9961 ttttgacaat taaaaatggt gtggcatctg aaaataccgg tgtcctttgc caccacggca
    10021 atcctctcct tagctcctcc gtctcgcgag tcctccacaa acctaagtgc cttcaagaga
    10081 gcatgtgggc ctaagtattt tttgtcctgt gatgctaccg ggcaggagcc atagcaagcg
    10141 ccacacagaa tacagtccag ccattcatct atcttgcgtc tctgttctac ggattgaata
    10201 tattcctttt gcggaggaac ttccttgggg ataagataag gttttatctc tttcaaatgc
    10261 tcgaagaaag gagtcagatc agtgattaaa tcctttatta ccggcaggtg agccaagggc
    10321 cttatggtta tcctggaccc caatacacct atttgggttt tacaggcaag gcgatacatc
    10381 ccatttacgt gcatagcaca ggaaccgcaa accgcctcgc ggcaggacga cctgaaagcc
    10441 agtgagccat ctatgttctc gaagatatgg aaaagcccct ccaaaatggt catccccttc
    10501 tcttcttcga acttatagga ttgaaaatgg ggctttcctc ctttatccgg gtcatacctg
    10561 aaaactctga attgtaccat attgtctctc cttgattatt aatattttct ctcttcgggc
    10621 ttgaatctgg ttatgaccac cggcctgtag gaaagcttgg gtccgtcagg agcatgagtt
    10681 gttaaggtgt gcttaagcca gttgacgtca tctcttttat tgaaatctgt gcggtaatgt
    10741 gaacctctcg tttcctgacg agccaaagcg gaaacagcca aagcttccgc cacatccaga
    10801 ttagacccaa cctcatagca acttatcaag tccatattgt aagctttccc ggaataagct
    10861 ggtcttatct ttttgaattt gtctttcagt tttcttattc cttccagccc ggctttcata
    10921 tcctcttctt tacggaaaag gccaaagcac ttaaccatca gattggctaa ttcatctctt
    10981 atcttgtagg gattttccgt tcctccaccc ttcttgcagt tctccagggc ttccctctgt
    11041 tttttcaaac aatcgcctaa tgctttctct ttaggtttct tgtctttacc tttaacccac
    11101 tccgctgcct ttttcccggc tctttcccca aacactatgg tctccaagag cgaatttccg
    11161 cccaaacgat tagctccatg aacactgaca caggcacatt caccagctgc caaaaacccc
    11221 tccattggcg tctgaccatc tttatccgta tcaattccgc ccatttcata atgtgtgccc
    11281 ggagttatgg gtatgggctc ttttatggga tcaattccca cgaagttcaa gcagatatcc
    11341 cgaattcccg gtaatctttc cattatctta gctgctccta aatgggttaa gtctaaatgc
    11401 accgtcccat ccggaaatcc tcttccctca tttatctcag tttgaatcga ccgtgccaca
    11461 atatctcttg gtcccagctc catcaccttt tcggagacgt acttactcat aaatctttca
    11521 ccaagtctat ttttcaaata acccccctct cccctacatc cctcggtcat aaggatgtgg
    11581 gttctggcta atgcagtggg atgaaactgc atgaactcta tatccttcaa aggaacccca
    11641 gcccagtatg gtatggccat agccagaccg gtattggtga tagcattagt ggtattggca
    11701 tataatctgc cgatccctcc ggtagcaaaa ataactgcat tcgctcccat agcatgaagc
    11761 tcgcctgaaa ggagttccat agcgataacc ccacagcatt ttccattctc cgtagctaac
    11821 cctaatacta tccattcctc atatactttg acattatgct tgatgttttg ttcccaggta
    11881 gtctgcaaaa ggtaaagccc ggtcttatca gtagcatagc aggttcgaga aaatccagcc
    11941 ccgccgaaag gacgctgggc gattcttccc gcttctgttc tggaaaatgg gcatccccaa
    12001 tgttccatct caaagatctt ttcgggaccc gagctggtta ggacctcaga cgcatcttgg
    12061 tctgctaaga agtcacttcc cttgaccgtg tcgaagaggt gcttttccgg agtatcatct
    12121 ttgcttcctt ccacattagc cagtgcagcg ttgatgcctc cctgagcttc acctgaatgg
    12181 gacctcatag gatgaatctt ggagatgata gctacatcca ctcccttttc cgctgctgca
    12241 atgcctgctc ttaagcccgc caatccgcct ccaactatca agatatcatg aaatatcata
    12301 ttcttcctta ctcctttttt aatgtccaaa tgcaagcggt aagagaacca tcaaagtcgc
    12361 caccatgaaa agggcaaaaa gaactgtgcc taaccagaac atcagcttat gctgcctggt
    12421 gacaaagaaa aagtcagcta tggtgactct gagtccattt aacatatgaa tggctacagc
    12481 cgctaataaa aatatctcca agaaatgagt taagggagtc tgtagatttt taagccatat
    12541 attgaaagtc tccgagcccc ataagccgga gcttactgcc aatatatgca taaaaagata
    12601 caaggtcaga actattccgg tcaacctctg caataaccag gaaaaagtcc cgatgttggc
    12661 atttaagctc cattcatcca aataatattt tatcgctcct gatttctgtt catacataga
    12721 tagctccttt tttatcgtct aaatggggtg atagcgtaga tggcgtagac agttgcaaca
    12781 acgcccagta tccacaggaa cgcttttatc gctttttgtg ctcctgggct gggccggtaa
    12841 tcgtgtatta tctgccacgc tccgttcagc ccgtgaaaca aaacaaccac aatgaagact
    12901 aagtagaata tcacccacca agaagagctt ccaagacgct ggctgacaac attaaaatcg
    12961 atgaactgcc tttggctgaa gtgcagaaca ttcagatgaa caacaacaaa gaaggccaaa
    13021 aggattccgg ttatcctctg ccatagccag ccggtggcag ttcccctctg taaatcttga
    13081 ctcatattag agaatctcct ttaactgaag gttgagtatc tcttgcctct tgtattttta
    13141 ttcctgaatt tactaggtag tggaacaatt gccagcttga gttgttattt ttttcacaag
    13201 aattatgtta ataattaagg ataattttaa cttaacgcaa caaaaaagtg aaatttcata
    13261 aaaaataaga gactgtctaa aaacgctctc tttcgcgttc ttgtcattgc gagcgaccgt
    13321 agggagcgtg gcaatctctt tgtttatcta atagttatga gattgcttcg tcgggctacg
    13381 ccctcctcgc aatgacactt cttagacagc ctcagacatt ccggtaaatc cgaaaacagt
    13441 ataggtctgt attattcggt gaaagaaata actttaactg tacccaactt ctccaaaccg
    13501 gctttcacct tttctgcatt agccactacc accatcttta aatcatccgg atgaatatac
    13561 tgctttgcag ctgttaagac ctcggccacg gtaacctgtt ttatattctt gcgatagttt
    13621 ttgatgtaat ctaaaccggt gccgtagatc tcggtatcaa gcacctgcgt agctacctgc
    13681 tcaggagttt caaacctcaa gggataataa ccggtgtaga aactcttggt atcatccaat
    13741 tctttcttcg taacttctgc ttctctgatt ttcttaagct cctccagaat ggctcctatc
    13801 gccgctaagc cagaatcaac ttgagtggac atactaacat cgaatgagcc tgggtcgcga
    13861 ttaagattaa aggagctata cactccatag gtcaaccctt tttcaactct gactttagcc
    13921 atcaaccttg acgaaaaacc gcctccaccc agaatatagt tcatcacttt ggatggaaac
    13981 tcatccgggt cttttctggc aagtcccaaa tgcccaattg atatataggc ctgggctaag
    14041 tcgggcttat ccaccaaaag tatatcgtac cccttgactt ccggtggaga tgcgaatttc
    14101 accacaggca gagatttctt ttgccaattt ccgaatttgg cttcaatttt ttttatgaca
    14161 tcctccgctt tgacatctcc caccacagcc agaacagcgt tattgggcac gtagaaggtc
    14221 ttatgaaact ctacgatgtc atctggggtg attgctgcaa cgcttttgtc atctccgctc
    14281 accggcctgg cataaggatg atctccaaac aggaactgct tgaatttctc ctccatgata
    14341 gtgttggggt catctttttt ctgaataata gcggccaggg tctgtctttt tagacgtttc
    14401 acttcactct cggaaaaggt cgggttcaga acaatatcgg aaagtaaatc cagcccagta
    14461 tcgaaatact tactcatcac tcggcaagta gcattgctgg cgtccaaatt cgctcccgct
    14521 cccaagctac ctcccacaaa atctatttct tgggcgatct gcggggcagt tctggtggtg
    14581 gttcccttgg ttaagagtcc agcggtcaga ttagctaaac cagctttgtc tttagggtca
    14641 taagccgctc ctgttttcaa aaccaagcgg aaggcgacca caggtaaatt atgctgctct
    14701 atcaccataa ccttcatgcc attctctagc accttggtct gtatctgggg cagttttatc
    14761 tcatccgaag tctcagccga caaaagagaa attgaagcaa gatacgaaaa acaaaaagcc
    14821 agtatgacca aggcaagggc ggtcttaaac atattcttat acatcttttt attcctcctt
    14881 tatctttgtt cagtcattgc agttggtgga gcttcgggaa taagtagagc tatagtcctc
    14941 tttctgggat cgagatattt cttggctacc ctcatcacat cctcaactgt aactgcctga
    15001 tatttctccc ctatctcaaa taggagcttg taatcaccat aaatcgcctc ataatgaccg
    15061 atttggaaac ccttattatc cgccccctgc aacccgaaaa caaactccga ctctaattga
    15121 ttcttggctt tctgcaattc cttttcagat actggctcag tacaaagtct atctatctca
    15181 gcaaatagag ctttttcccc ttcctttaag tctctgcccg gttgcattcc cacaaaagca
    15241 tagaaaactc cggcatcctg taatatccag gcaccaccat ccacgaacag cgccatctgc
    15301 tcgtcgtaga ccatcttctt atagactctc gagctttgac cgctgaagag aatcttagct
    15361 gccacttcaa aaggataact gtctggatcc cctaatttgg gaactttata actaactgcc
    15421 aaaccaggaa gctgggctat cttgtgaacc tcaattctcc tttcacctaa ctgctccggc
    15481 tcaatctctg tcaccttagt gggagcagga cccgctttga ttttgccaaa atatttcttg
    15541 gtcaacttca gcgcatcttc tgctttaata tcaccggcaa caacggctac ggcattatta
    15601 ggtacgtagt aggttttgaa gtggttcttg agttggtcga aggtccagct ctgaatatct
    15661 gacatgaatc cgatgactat ccagtgatat ggatgagcta caaaggtgtg attgaggagc
    15721 tgttcaaaaa ctgcgtcaaa aggctggtcg tctaaggtct gacgtctttc ctcggctatc
    15781 acattgcgtt ctggttgaaa atcctcattg gcgagtttaa ggtttttcaa gcggtcagcc
    15841 tccaagctca taactagttc caatttgtcg ccggaaacct tttcccagta agtggtattg
    15901 tcaaacgaag tccaggcatt aactatgccg ccgttagctt gtactatttt ggagaactcc
    15961 tctggtctga ctttcctcgt ccccttaaac atcatatgct cacacatatg agatatgccg
    16021 gtgattcccg gacgctcata ttttgagcca accttatacc atatggaaaa agaagccact
    16081 ggagcggagt ggtcttcagc caccaacaag gtcagcccgt tatccagagt aaacttctgg
    16141 acatcaaaat gcacctgggc tgagcctatc tgcgtcagga agagaagcaa gagcaaggat
    16201 agggctaacc tatttaatgt tttaaacaaa agtacctccg gttaagggtt taggatttac
    16261 ttgattatgg gaaattactc acctctgcca gaaagtttcg ctcgcctctc ggcgctcgcc
    16321 tctcggcgaa gccgagggaa gccgaggacc ttttatattt gggaagctcc ctgatttatc
    16381 acggggaggc ttcactttcc gacgttgtgt attgtgagac attatacaca gcagttcgtg
    16441 ttttcgcaac tattttgttc ggaccaggtg aagacaattt atcaatattt aatacgtctg
    16501 aatcaagccg atgtttcagt tagtttgaat attgccatct gatacatcag gatgccgcca
    16561 gccaccgaag cattcaaaga ttctgctttc ccgtaaattg ggatattggc tatcttatcg
    16621 gcgagctttg agatttcagc tctaactcct ttgatttcat tgccgaccac gagacaaact
    16681 cttccggaat aatctatctt gtgataaggc tcccctcctt taactgcgct ggcaatgatt
    16741 ttgaaccctt cctcttttaa tctccgcaag gatttgacca aatccgtttt ttctgcagta
    16801 cgaagatgaa agatcgaacc catggttgaa cgcacaacct ttgggctgta aagctctact
    16861 gttttttctg ataagataac cccatttatc cccgctgcat ctgcagttct aattaaagtc
    16921 cccagattcc ccggatctcc gacttcatct aagaccaaga tgctctgtgg attccctttc
    16981 aggaggtcat ccaatttaag gtctctggtt ctcaccacgc atactatccc actagagctg
    17041 actgcttcag atatcttatc caagatatcc tgactgattt tttcaattgc tatcttcctg
    17101 ctggtagctt tccgtaagat gaagttagcc ctatcgcttt tttcaagcaa gctagaataa
    17161 agtagtactt ccacttccca atccgaactc aaaagctctt caactgacct gaccccttct
    17221 accatgaact tcccttgctt ttgcctgaat ttcttgttct tcagtttgag aatctctttg
    17281 gatttccttt gggaaaacat aaatttatcc tttagtttga ccgggtcaag atagttaaaa
    17341 ccgaagcaat tgtcaatctg agctttcgct tgcttttggt tgagaagtct atataattat
    17401 agattgcatt tctcgaataa agaaccccgc ccacagggcg gggctttaaa acccaaaccg
    17461 agatcccgaa ccaagttcgg gatgacaaaa accgtaaaac caaaccctgt tgtcatctgg
    17521 tctgtttttt tctgcttcca gcagacgcac ctttcatcac cgtccacgaa gacggggtct
    17581 gcaggtgcga tcttaataaa acttggtaaa atgaaaagaa aaacgataat ctttctctta
    17641 acttctttgc ttttttctag tgtctgggca gaaggtaaga caattcgtct ggaatttgga
    17701 gaacccaatt cccagagcat aaatctccct ctaaaaaaac agctcaaaag aaagaaagtg
    17761 ggcttagccc tctccggtgg tggcgcccga ggtttcacgc aaattggaat actgaaggtc
    17821 ttagaaagag aacaaattcc aatcgactat attgccggaa ccagcatggg tggagtggta
    17881 ggcggactgt tcgcagctgg atacagtgct gaagagttag aagaattagt tctgaaaaca
    17941 aactgggaag atatcttaag agacaatcct aaccgcctat ctttattttt gagccagcgc
    18001 gaggaaagcg aaggatattt cctgaagata cgtttcaacg gattcagacc atatatgcca
    18061 accgcgctta cttctggcca gaagatcact gatcttttca ccagcttgac tgctggagct
    18121 aactaccggg ctcgggctaa cttcgattac ctgaaaatac ctttcagggc ggtggcaact
    18181 gatctggaaa ctggacagaa ggttgtattg ggctcaggcg atttagcaga agccctaagg
    18241 gcaaccatat ccgtgccctt ggcttttgct ccagtggaat tagaccagaa agtattagtg
    18301 gacggagggc tggttgagcc aataccagtt gagactgcca aggagatggg ggcaggaata
    18361 ataatcgcag tcaacacatc caccagtctt ttgagttgga agaagataac aaatcccata
    18421 gacattgctg accaaaccac cagcataatg tctttagatg agaaggagag gcagctatct
    18481 caagcggatt tggtcatatc tcctgaacta gggggttatt ctgcggttga tttcgataaa
    18541 gccaaggaat tgatcgccat aggagaaagg gcgtgtgatc aggtagtaag ccgaataaaa
    18601 cagatagttt ctgagaaaga cactttgggt caagagacgt attatttgac tgaagttgaa
    18661 ttcgatgggg cagaacaaat aaataaggat actctagaaa gaatatgtcc catccaagca
    18721 ggcgattcca tcactttgaa gtcgattgag gattatttaa gagagatata tggaagtgga
    18781 tatttttcag atgcttatgc agagctcgag ccttaccggt caggttatgt tttagtcttc
    18841 aatttgaagg aaaaccaaat cttaagagaa attctcttct cgggaaacag tatattctca
    18901 acctctcaac ttaaaaagga actatcctta gagctgggcc aaactctaaa ctctcagaga
    18961 ctagaaagag gactggataa tgtcttggaa ctatataaac agaagggtca cgccttagcc
    19021 agcataagta aggtcaattt caactccggg gctctactca tagaaataaa tgaagggatt
    19081 atatccgaaa taagagtcga gggaaatcat cgcaccaaga actgggtcat tttaagaagt
    19141 tttcctttaa aggtaggaaa accattcaat tcaaatctgg cggacaaggg tgttgaggat
    19201 atctatgcaa ctgaactttt cgacaaggtg acgttgaaaa tacttccggg cgaaaagggc
    19261 gcaattttag atataagcat caaggaaaaa gatttcactt tcctgcgaat aggaatgcac
    19321 tacgatgatg agcataatct ggaatctgca ctacagctga tggatgctaa tattctggga
    19381 atcggaaaca aaacctatgc cgacttactg tatggtaaca caaagcaaag atatggctta
    19441 catttcaaag cggatagaat atataaatcc tatcttacct acaggggtga catttttcat
    19501 caacgagaaa aaaagaagat ttactccgag catagagaga tagactcttt cagggagtgg
    19561 agaaccggaa cggttttttc attaggacaa catatctcac gcttgggaac cctgtcatta
    19621 gaaacaaagc tggaaagagt tgaattaaga gaaggaaaaa tatatccaaa agaagttaat
    19681 gatgttcgaa gcatcacctt gcgttccctg gttgatacct tcgataaata tccttttccg
    19741 gataggggaa aatatcatca tctttatata gaactggctg gcaggatctt gaagggagat
    19801 ttgaccttca gaaaaggttt cacctccctg gagtcctatt ttcctttgag caaacattgg
    19861 aattttcatc ccaaaatagc acttggaata tcggatggaa atctgcccac cagcgaacaa
    19921 ttccttttgg gcggatataa tgagttttac ggattcttcc aggatgagct aagaggcgat
    19981 aagttgattt atggtaatct ggaaatgagg ttcaaattct ggcagaggtt ttactggaat
    20041 gtaagatatg atattggga
//
LOCUS       DASWLN010000024         8331 bp    DNA     linear   ENV 20-FEB-2024
DEFINITION  MAG TPA_asm: Candidatus Zixiibacteriota bacterium isolate
            SMAG_U2949 k141_22103, whole genome shotgun sequence.
ACCESSION   DASWLN010000024 DASWLN010000000
VERSION     DASWLN010000024.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37960594
            Sequence Read Archive: SRR10376091
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Candidatus Zixiibacteriota bacterium (soil metagenome)
  ORGANISM  Candidatus Zixiibacteriota bacterium
            Bacteria.
REFERENCE   1  (bases 1 to 8331)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 8331)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (26-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/12/2024 08:28:26
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 1,440
            CDSs (total)                      :: 1,416
            Genes (coding)                    :: 1,412
            CDSs (with protein)               :: 1,412
            Genes (RNA)                       :: 24
            rRNAs                             :: 1 (16S)
            partial rRNAs                     :: 1 (16S)
            tRNAs                             :: 21
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 4
            CDSs (without protein)            :: 4
            Pseudo Genes (ambiguous residues) :: 0 of 4
            Pseudo Genes (frameshifted)       :: 0 of 4
            Pseudo Genes (incomplete)         :: 3 of 4
            Pseudo Genes (internal stop)      :: 1 of 4
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..8331
                     /organism="Candidatus Zixiibacteriota bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_22103"
                     /isolate="SMAG_U2949"
                     /isolation_source="soil"
                     /db_xref="taxon:2053527"
                     /environmental_sample
                     /country="USA: Oregon"
                     /lat_lon="42.99 N 123.63 W"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            <1..552
                     /locus_tag="VGB16_00805"
     CDS             <1..552
                     /locus_tag="VGB16_00805"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEX9916250.1"
                     /translation="VFVLMSLIICSNTVFGQANIEKGATEIGGNLFFISSSLSYQGHS
                     DGSVSFFLLAPRFGFFKTDNFEVEPQLLYGRVSENPEYGSSYSFSACGGILSFSYNFK
                     TSSNIVPFLFAGVGFLSYSGGGDSQLSAIAPDAGIGMKSFISDKGALRLELFYQNMAN
                     YMGIEDLLASNFGFRGGFSVFLK"
     gene            773..846
                     /locus_tag="VGB16_00810"
     tRNA            773..846
                     /locus_tag="VGB16_00810"
                     /product="tRNA-Arg"
                     /inference="COORDINATES: profile:tRNAscan-SE:2.0.12"
                     /note="Derived by automated computational analysis using
                     gene prediction method: tRNAscan-SE."
                     /anticodon=(pos:807..809,aa:Arg,seq:tcg)
     gene            951..1025
                     /locus_tag="VGB16_00815"
     tRNA            951..1025
                     /locus_tag="VGB16_00815"
                     /product="tRNA-Glu"
                     /inference="COORDINATES: profile:tRNAscan-SE:2.0.12"
                     /note="Derived by automated computational analysis using
                     gene prediction method: tRNAscan-SE."
                     /anticodon=(pos:987..989,aa:Glu,seq:ttc)
     gene            complement(1176..1874)
                     /gene="hypB"
                     /locus_tag="VGB16_00820"
     CDS             complement(1176..1874)
                     /gene="hypB"
                     /locus_tag="VGB16_00820"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_010939602.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0000166 - nucleotide binding [Evidence
                     IEA];
                     GO_function: GO:0016530 - metallochaperone activity
                     [Evidence IEA];
                     GO_process: GO:0036211 - protein modification process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="hydrogenase nickel incorporation protein HypB"
                     /protein_id="HEX9916251.1"
                     /translation="MRKIKVLTNFLEANDAVALENKKLFDKHKILVLNLMSSPGAGKT
                     LILEKTIEKLKKHFKFAVIEGDVATSNDAKRLAKYDIPVVQITTESFGGACHLDASMI
                     NKAIQGLKLSSIQIIFIENIGNLICPAEFDLGEDRKVIVLSLTEGEDKPSKYPVVFRN
                     SDLLLINKVDLLPQLDIDVDLILKNTKKVNHKLPAIQLSAKTEQGFKKWLDWLVKEYK
                     SKPKRKVCSGKWRE"
     gene            complement(2161..2505)
                     /gene="hypA"
                     /locus_tag="VGB16_00825"
     CDS             complement(2161..2505)
                     /gene="hypA"
                     /locus_tag="VGB16_00825"
                     /inference="COORDINATES: protein motif:HMM:TIGR00100.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008901 - ferredoxin hydrogenase activity
                     [Evidence IEA];
                     GO_process: GO:0036211 - protein modification process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="hydrogenase maturation nickel metallochaperone
                     HypA"
                     /protein_id="HEX9916252.1"
                     /translation="MHEMAVAENILKVIEEKLRKIDQKGEVKKVNLKIGKLTCIEPEA
                     LRLSFEVISKETPFEKASLFIDSVPITGICKDCTKELLLEKTDFTCPACGSFRVEIKT
                     GRELFIESFEIE"
     gene            complement(2514..3539)
                     /gene="hypE"
                     /locus_tag="VGB16_00830"
     CDS             complement(2514..3539)
                     /gene="hypE"
                     /locus_tag="VGB16_00830"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_007219965.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hydrogenase expression/formation protein HypE"
                     /protein_id="HEX9916253.1"
                     /translation="MTNKHKSNEKITLSHGAGGKLSFDLISHLIRGKFSNQILNRLED
                     SAVFNLGKEKIAFTTDSYVIQPIFFPGGDIGKLAVCGTVNDLAVMGARPLFLSCSLII
                     EEGLDQAILEKVILSMKKTAQKAKIQIVTGDTKVVEKGSADNIFINTSGIGIVSKGIN
                     LGKERIKIGDKIIISGTIGDHATAVLSSREGLEFQTKLKSDCDFLSDLIGSVLKFKDK
                     IKFMRDPTRGGLASVLNEMVSDKSFGAILFEDKIPIKEKVKGFCELLGYDPLYLANEG
                     KVLIITAPEIAEKVVSILRKHPKGKDAEIIGEITDKFKGIIGLETKFGGTRVVDMLLG
                     EQFPRIC"
     gene            complement(3561..4706)
                     /gene="hypD"
                     /locus_tag="VGB16_00835"
     CDS             complement(3561..4706)
                     /gene="hypD"
                     /locus_tag="VGB16_00835"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_010940977.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016530 - metallochaperone activity
                     [Evidence IEA];
                     GO_process: GO:0036211 - protein modification process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="hydrogenase formation protein HypD"
                     /protein_id="HEX9916254.1"
                     /translation="MNSNLFDEFSDSRVTKHLSEKIKHTANQIKREVTLMEVCGTHTV
                     AIFKCGIKSILPHNIKLISGPGCPVCVTPNEYLDRAIALSQLEDTILVTFGDMMKVPG
                     SSSSLEKEKTHGADVRIVYSTLDALKIAKENRDKKVVFLGVGFETTTPTIAFAMKEAK
                     KKQINDFFVLSGHKLIPPAMRFLAQSKDLRVDGFICPGHVSTIIGTKPYEFLAKEFDL
                     PCVIAGFEPLDLFQSLYILLNRIKNRDPRIDNQYFRAVKRDGNPIALNLIDSVFEKED
                     SFWRGIGIIPQSGLKIRDEYEEFDAFKKIDAKVEKSQEPKGCLCGLVIQGKKTPPDCT
                     LFAKKCTPASPIGPCMVSSEGSCQAYFKYGQRPKVKPQDTRKKKRKH"
     gene            complement(4699..4914)
                     /locus_tag="VGB16_00840"
     CDS             complement(4699..4914)
                     /locus_tag="VGB16_00840"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_012635337.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="HypC/HybG/HupF family hydrogenase formation
                     chaperone"
                     /protein_id="HEX9916255.1"
                     /translation="MCLGVPVRIIQKKDREGIAEFKGVKKEVNLDLLDEVEVGDYVIL
                     HAGFAIQKLDKKEALETLKLFEKLSNE"
     gene            complement(4916..7183)
                     /gene="hypF"
                     /locus_tag="VGB16_00845"
     CDS             complement(4916..7183)
                     /gene="hypF"
                     /locus_tag="VGB16_00845"
                     /EC_number="6.1.2.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_013120178.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003998 - acylphosphatase activity
                     [Evidence IEA];
                     GO_process: GO:0036211 - protein modification process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="carbamoyltransferase HypF"
                     /protein_id="HEX9916256.1"
                     /translation="MYSIKLEIKGIVQGVGFRPFVYNLAQKHRIRGYVLNDTRGVEVK
                     AEGKKKDIDKFILQIRTSPPPQSQIEEVKLERISFEGYKSFVIRESRKKNIKTVLVSP
                     DLSTCDDCLKELFDPGDRRFGYPFLNCTNCGPRLTIIQDIPYDRNKTTMSVFKMCPDC
                     NREYNNPENRRFHAQPNACPICGPRLLLMDKKKNVIHTTNPISSAIQLLKKGYIVAMK
                     GIGGYHLACDATNKNAVSTLRKRKYREDKPFAIMAGDIKIIQKFCHVNSLEKKLLQSV
                     SRPILLLKKKKGDLIASDVAPLQKNWGVMLPYSPLHHLLLGKSNLILVMTSGNQSDEP
                     ISFQEDDVFEKLSGIADYFLIHNREIHRRCDDSVTRIWKDREMILRRARGYVPRPIKV
                     DFKFKKHILAAGAQLKNTFCLAKDGFAFLSTHIGDLENFETLDFYTKEIERFKKLCSV
                     KPEIVAYDLHPEYLSTKYALSSSNLKKIPVQHHYAHIASCMAENNISQKVFGVAFDGT
                     GFGEDGAVWGGEFLLADFNGYKRVAHLKYVPMPGGEMAIKQPWRMALSYLYTCWGEDF
                     LNLDLEFTRKVSRAKWETIKKMINQNINVFPTSSMGRLFDCVSVLVNWRDQTNYEGQP
                     AIELEVLADDKTKTKYDFEVIEKEGIFIIPPEPTIKGVVKDLIRKEEKSRISAKFHNT
                     IAWMINEVCLGLRKKYELNSVCLSGGVFQNIVLLDKTYKLLTKNGFKVYTHHKVPTND
                     GGISLGQVAVANHRI"
     gene            7411..>8331
                     /locus_tag="VGB16_00850"
     CDS             7411..>8331
                     /locus_tag="VGB16_00850"
                     /inference="COORDINATES: protein motif:HMM:TIGR00391.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008901 - ferredoxin hydrogenase activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="hydrogenase small subunit"
                     /protein_id="HEX9916257.1"
                     /translation="MALPRRKFLQLVGGSAAAVGLSNLWLPKVVQAILEKPGNPAVIW
                     LQAQSCTGCSESLLDTTYPDIAKVLTEIISLQFHPTVMASAGDPAIKILDDILQNQKG
                     KFILVVEGSIPTEASGAFCTVGEKDKKPISILDWTMKFGNASKAILNVGTCSAFGGIP
                     AGKPNPTGAKAVKDILPNATMINIPGCPPHPDWMVGTIAHVLLYGLPELDDQLRPVIF
                     YGGFIHENCERRAYFEEGKFAQDYSDEGCLFELGCKGPISHCDVSIRGWNNGVNWCCR
                     SGGPCIGCTEPTYPDHEGAGLYGLLDKKSIL"
BASE COUNT         2267 a         1790 c         1655 g         2619 t
ORIGIN      
        1 gtttttgtac tgatgtcttt gataatctgt tcaaacactg tcttcggaca ggcaaatatt
       61 gagaaggggg ctacagagat agggggtaat cttttcttta tttcgagttc cctttcttac
      121 caaggtcact ctgacggttc agttagtttc tttctgcttg cccccaggtt cgggtttttt
      181 aaaacggata attttgaggt tgagccacaa ttgctttacg gaagagtcag cgagaaccca
      241 gaatatggtt cgtcgtacag cttcagcgcc tgtggcggaa tcttgagttt tagctataat
      301 tttaaaacct caagcaacat agtgccgttt cttttcgcag gtgtggggtt cttgagttat
      361 tcaggaggag gtgactctca gctttccgct atagcacctg atgcgggaat agggatgaag
      421 agtttcatta gcgacaaggg tgcgctgaga ctggaacttt tttaccagaa tatggccaat
      481 tatatgggca ttgaggattt actagccagc aatttcggat ttagaggagg tttttcggtt
      541 tttctaaagt gaattgcata agtagtagtt gtgctgttgt tcgttcttaa atggatatgt
      601 atattatata atgttgtgag aagaaagcgt aggggcgaag catatctatc gcccgaaaaa
      661 tgcggatgca gatgaattcc accctacgcg gttttgtagg cggggctccc tagcctcgcg
      721 gttgggcaag gtgcccaacc tacgaaagaa aaatgtgaaa gagaaacatt ttgcgcctat
      781 agctcaattg gatagagctt cggacttcgg atccgagagt tgggggttcg agtccctcta
      841 ggcgcgattc cgacgaagtc ggaataattc acagttgaca gttcacggtt cgcagctcaa
      901 aaagtttttt ctgtgaactg tgaacggata accgtgaacc agcaaagctc gtccccatcg
      961 tccagcccgg tccaggacac cgccctttca cggcggcgac acgagttcga atctcgttgg
     1021 ggacgttttt tcttgttcaa tattaggtga tgtcgtactt agcggaactg aatagcggtt
     1081 gttcataaaa caattcagaa atcatatagt aactcttagg gtcggcatcg ttcaagattt
     1141 gtgatttgtg aggtctagac catacctgaa aaagattatt ccctccattt tcctgagcat
     1201 actttccttt taggtttaga tttatattct ttcaccaacc agtcgagcca cttcttaaat
     1261 ccttgttctg tcttagcgga gagttgaatg gcgggaagtt tatggttgac ctttttggta
     1321 tttttcagaa ttaaatccac atctatatcc aattgaggca agaggtcaac tttgttaatt
     1381 aaaagcaaat cggaatttct gaaaaccacc ggatatttgc tgggcttatc ctccccttcg
     1441 gttagactca agacgatgac ttttctgtct tcccccagat cgaattcagc cggacaaatc
     1501 aagttgccga tattctcgat aaatattatc tgaatagagg acaatttcag gccctgtatg
     1561 gctttgttta tcatactggc atcgaggtgg caggcaccgc caaaagactc agtggttatc
     1621 tgaactacgg gaatatcata cttagccaac cttttggcat cgttggaagt cgccacatcg
     1681 ccttcaatca ccgcgaactt aaaatgcttt ttcaattttt cgatagtttt ctccaaaatc
     1741 aaagttttac ctgcacccgg ggagctcata agatttaaaa ccagaatttt gtgtttatca
     1801 aaaagtttct tattctccag ggctacggca tcgttggctt caagaaaatt agttaaaact
     1861 ttaatctttc tcatatttcc tcctctaatg cggatatatc atttatgaaa gcagtaccag
     1921 aatttccccc gcagggggat aaatgacatt tctataagtt ccctctccct tgggggagag
     1981 ggttagagcc tgccctaaag cgaagccctg caccagattg gtacagggcg aagcgaaggg
     2041 gtgaggggga tagcgaagac gattctcacc ctctctccgc ctttggcgag attttcaact
     2101 tagtcctccc cctcaagggg gaggagatgt tttagatgct ccactctttg ggcggagtat
     2161 tcactcgatt tcaaatgatt ctatgaaaag ttctctgccg gtttttatct ctactctaaa
     2221 tgagccacaa gcagggcagg tgaaatctgt cttctctaac aaaagctctt tagtgcaatc
     2281 tttgcatatc ccggttatgg gaacggaatc aataaagagg gatgcttttt caaaaggggt
     2341 ttctttggag atgacttcaa aagataatct taaagcttcc ggctcaatgc aggtgagctt
     2401 tccgattttc aagttgactt tttttacttc ccctttttgg tctatctttc tcaatttctc
     2461 ttcaatcact tttaagatat tttccgctac tgccatctcg tgcatatcac tcctcaacag
     2521 attcgtggga attgttcacc caaaagcata tccaccactc tggttccgcc gaactttgtt
     2581 tccaatccta taatcccttt gaatttgtcc gttatctctc cgataatttc agcatctttg
     2641 cctttggggt gttttctcaa gatgctaacg actttttccg ctatctctgg tgcggttata
     2701 atcaatacct ttccttcgtt tgccagatat aatgggtcat atcccaaaag ctcacagaat
     2761 cccttaacct tttctttaat gggaatttta tcctcaaaca gaatagctcc aaagctcttg
     2821 tctgaaacca tctcatttaa taccgaagcc agaccaccac gagtggggtc gcgcatgaat
     2881 tttatcttat ccttaaattt cagaacagaa ccaatcaaat ctgaaagaaa atcacaatca
     2941 cttttcaatt tggtttgaaa ttccaaaccc tctctcgaag ataaaaccgc tgtggcatgg
     3001 tcgccaatgg ttccgctgat tataatttta tccccaatct ttattctctc ctttcccaaa
     3061 tttattcctt tcgaaacaat accaatgccg gaggtgttta tgaagatgtt atctgcactt
     3121 cctttttcca ccaccttggt gtccccggtt actatctgga ttttcgcttt ttgagcggtt
     3181 ttcttcattg ataagattac tttctccaag atggcttggt ccaacccctc ttcgataatt
     3241 aaagagcaag ataagaaaag tggtctcgca cccattactg caaggtcatt tacggtcccg
     3301 caaactgcca atttgccaat atctccacca gggaagaata tgggctgaat cacataagag
     3361 tcggtagtga aagcaatttt ttccttgccc agattgaaaa ctgccgagtc ttccaaccta
     3421 tttaagattt ggttactaaa tttacctctg attaaatgag aaatcaggtc aaaagagagt
     3481 tttcctccgg caccgtggga gagagttatc ttttcgttcg atttgtgctt gtttgtcata
     3541 acaaatacca agacggtcaa ttaatgtttt ctcttttttt tccgcgtatc ttgtggttta
     3601 accttaggtc tttgtccata cttgaaataa gcctgacaag agccttcaga ggataccata
     3661 caaggcccaa taggggatgc aggggtgcat ttcttggcaa aaagggtgca atccggagga
     3721 gttttcttgc cctgaattac caaaccacac aaacaaccct taggctcctg acttttctca
     3781 actttagcat ctattttttt aaaagcatca aattcctcat attcatctct aatcttaagt
     3841 cctgattgtg gtataatgcc gatgccccgc cagaatgaat cttctttttc aaaaacgctg
     3901 tcgattaaat tcagggcgat tggattccca tctcttttta ctgcccgaaa atactggtta
     3961 tcgattctgg gatctcggtt ttttatccga ttcaacaaaa tatataaaga ttggaacagg
     4021 tccagcggct caaatccagc aatcacgcaa ggtagatcga actctttggc taagaattcg
     4081 taaggtttag ttccaatgat ggtgcttaca tgtcctgggc aaataaaccc atccactctt
     4141 aaatctttgg actgggctaa aaacctcata gccgggggga ttaacttatg accggataaa
     4201 acaaagaagt catttatttg tttctttttc gcttccttca tagcaaaagc gattgtggga
     4261 gtggtggtct caaacccaac tcccaggaag accactttct tatctcgatt ttcttttgcg
     4321 attttcaaag catccaaagt ggaataaact attcttacat ctgctccatg ggttttctcc
     4381 ttttccagag aggaagagga gccgggtact ttcatcatat ccccgaatgt gaccaggata
     4441 gtgtcctcta attgtgataa ggcgatggct ctatcgagat attcattcgg agtaacgcag
     4501 accggacatc caggaccgga gatgagtttt atattatgtg gcaaaatgct ttttatccca
     4561 cacttgaaaa tagccacagt atgtgttcca cagacctcca tcaaggttac ttctcttttt
     4621 atctggttgg cagtatgctt aattttctct gacagatgtt tagttaccct ggaatcagag
     4681 aattcatcaa aaagattgct attcattaga taatttctca aataatttca gagtttctaa
     4741 agcttctttc ttatccagtt tttgtatagc aaatccagca tgcagaatca cataatctcc
     4801 tacttcaacc tcatctaaga ggtccaaatt cacttccttt ttcacccctt tgaattccgc
     4861 aattccttcc ctatcctttt tttgaatgat tcttaccgga actcccaagc acatatcaaa
     4921 tcctgtggtt agctactgcc acttgtccta aagatattcc accatcattg gtgggaactt
     4981 tatgatgagt gtagacctta aatccgtttt tggtcaaaag cttatacgtt ttatctaata
     5041 gaacaatatt ctggaaaacg ccaccggata aacagaccga attcaattca tatttctttc
     5101 tcaatcccaa acaaacctca tttatcatcc aggcaatggt attgtgaaac ttggctgaga
     5161 ttcttgattt ctcttctttt cttatcaagt ctttcaccac acctttgatt gtgggctccg
     5221 gaggaatgat gaatatacct tctttttcga ttacctcaaa atcatatttt gttttcgttt
     5281 tatcatctgc caaaacttcc aattcaattg ccggctgacc ttcgtaattg gtctggtctc
     5341 tccagttcac taaaactgag acgcaatcga aaagccttcc catgctggaa gtaggaaaaa
     5401 catttatatt ttggtttatc atctttttta tggtttccca tttagcccta ctcacttttc
     5461 tggtaaattc cagatccaga ttcaaaaaat cctctcccca gcaagtgtat agatatgaca
     5521 gagccattct ccagggctgt tttattgcca tctctccgcc aggcatagga acatatttga
     5581 gatgagccac tcttttatat ccattgaaat ccgctaaaag aaactctcca ccccagacag
     5641 ccccatcctc tccaaatcca gtcccatcga aagccacccc aaacaccttt tggctgatat
     5701 tgttttcagc catacagctg gcaatatgag cataatgatg ttggactgga atcttcttaa
     5761 gattcgacga agatagcgca tatttagttg agagatattc gggatgcaaa tcgtaagcca
     5821 caatctccgg cttgaccgag cagagctttt tgaatctttc aatttcttta gtataaaaat
     5881 ccagagtctc aaaattttcc aaatcaccaa tatgagtgct caaaaaagca aaaccatctt
     5941 tagctaagca gaaagtattc ttcagttgag cacctgcggc taagatatgt ttcttgaatt
     6001 taaaatcgac ctttataggt cgggggacat accccctagc tcttcgcaat atcatttctc
     6061 tatctttcca gattctggtt accgaatcat cacagcgtct atggatttct cgattgtgaa
     6121 ttagaaagta gtcggcgatg ccacttaact tttcaaagac atcatcctct tgaaaggata
     6181 ttggctcatc cgactgatta cctgaagtca taaccaaaat cagatttgat tttccaagca
     6241 ataggtggtg taagggggaa tagggaagca tcacccccca gtttttttga agaggtgcga
     6301 catccgatgc aatcaaatca ccctttttct ttttcaaaag caaaatcggt cgagaaactg
     6361 attgcagaag ttttttctca agtgagttca cgtgacagaa tttctgaatg atttttatat
     6421 ctcctgccat tatagcaaag ggtttatcct ccctgtattt tctttttctc aaagtcgaaa
     6481 ccgcattctt gtttgtagca tcgcaagcta agtggtatcc accaattcct ttcatagcta
     6541 ctatataacc ttttttgaga agttgaattg cagatgaaat tggattagtg gtatgtatca
     6601 catttttctt cttatccatc aatagcagtc ttggtccaca aattgggcag gcattgggct
     6661 gggcgtgaaa tcttcggttt tctggattat tgtattctcg attgcaatcc gggcacatct
     6721 taaaaactga cattgtggtt ttatttctgt cataaggaat atcctgaata atggttaatc
     6781 tcggaccaca gttggtgcaa ttcaaaaatg gataaccaaa tcttcgatcg ccagggtcga
     6841 acaactcttt taggcaatca tcacaggtgg ataggtccgg ggagactaag acggtcttaa
     6901 tattcttttt tctgctttcc ctgataacaa aactcttata accttcaaaa gagattcttt
     6961 caagcttaac ttcttcaatc tgtgattggg gtgggggaga agttcttatc tggaggataa
     7021 atttatctat atcttttttc ttaccctcgg ctttgacttc tactccacgg gtgtcattca
     7081 aaacatagcc acgaatgcgg tgcttttgag ccaggttata aacaaagggc ctgaaaccta
     7141 ccccttgaac aatccctttt atttcaagtt tgatgctata cataaaccta caatataaac
     7201 tgaattagag aggtgtcaac ttcataatta ttacgaatca tagggaaagt atgtaaaaat
     7261 attcaaccag ggtttgtgaa aaaaacgaca aagtttgata taaattgaaa attttgattg
     7321 acaaattgag tatcaaatat cattattgtt aaataattca caagaccttt tgttttttca
     7381 taaaaaatat aactaaatta ggaggtcttt atggctttac ctcgaagaaa gttcctccag
     7441 ttagttggcg ggtcagccgc agctgtggga ctctctaatc tttggcttcc caaagtagtt
     7501 caggctatct tagagaagcc gggcaatcca gcagttatct ggttacaagc gcaaagttgc
     7561 accggatgtt ccgaatccct tttggatact acctaccccg atattgccaa agttttgacc
     7621 gaaataataa gcttacagtt tcatcctacg gttatggctt cagccggtga tcctgccata
     7681 aaaattctgg atgatatttt gcaaaaccaa aaagggaaat tcatcttagt tgtggaaggt
     7741 tcaattccca cggaggcttc tggcgctttc tgtactgtgg gagaaaaaga taagaaacct
     7801 atatccatct tggattggac gatgaagttt ggcaatgcct ctaaagccat cttgaatgtc
     7861 ggaacttgtt cagcttttgg cgggattcca gctggaaagc cgaatcctac tggagctaag
     7921 gcagttaagg atatcctgcc aaacgctacg atgattaata ttcccggatg tccacctcat
     7981 cctgactgga tggtcgggac gattgcacac gttttattgt acgggctgcc agagctggat
     8041 gaccagcttc ggcctgtgat tttttatgga gggttcattc acgagaactg tgagaggaga
     8101 gcctactttg aggaagggaa gtttgcccag gactatagcg atgagggttg ccttttcgaa
     8161 ttaggctgta aaggaccgat atcacactgt gatgtctcca taaggggatg gaacaacgga
     8221 gtgaattggt gctgccgtag tggcgggcct tgcataggat gcacagagcc aacctatcca
     8281 gaccacgagg gagcaggatt gtatggtctt ttggataaga aatctatctt g
//
LOCUS       DASWLN010000025         3844 bp    DNA     linear   ENV 20-FEB-2024
DEFINITION  MAG TPA_asm: Candidatus Zixiibacteriota bacterium isolate
            SMAG_U2949 k141_275296, whole genome shotgun sequence.
ACCESSION   DASWLN010000025 DASWLN010000000
VERSION     DASWLN010000025.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37960594
            Sequence Read Archive: SRR10376091
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Candidatus Zixiibacteriota bacterium (soil metagenome)
  ORGANISM  Candidatus Zixiibacteriota bacterium
            Bacteria.
REFERENCE   1  (bases 1 to 3844)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 3844)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (26-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/12/2024 08:28:26
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 1,440
            CDSs (total)                      :: 1,416
            Genes (coding)                    :: 1,412
            CDSs (with protein)               :: 1,412
            Genes (RNA)                       :: 24
            rRNAs                             :: 1 (16S)
            partial rRNAs                     :: 1 (16S)
            tRNAs                             :: 21
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 4
            CDSs (without protein)            :: 4
            Pseudo Genes (ambiguous residues) :: 0 of 4
            Pseudo Genes (frameshifted)       :: 0 of 4
            Pseudo Genes (incomplete)         :: 3 of 4
            Pseudo Genes (internal stop)      :: 1 of 4
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..3844
                     /organism="Candidatus Zixiibacteriota bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_275296"
                     /isolate="SMAG_U2949"
                     /isolation_source="soil"
                     /db_xref="taxon:2053527"
                     /environmental_sample
                     /country="USA: Oregon"
                     /lat_lon="42.99 N 123.63 W"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            <1..272
                     /locus_tag="VGB16_00855"
     CDS             <1..272
                     /locus_tag="VGB16_00855"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=3
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEX9916258.1"
                     /translation="QEEFNAGRERSWNMIYGIKGINIPSVEGIDIKLAYYYPEGVYIG
                     YTISKAYYFPQSRYILVFINQPRMASGLDTMHGFLLLKIVKESGG"
     gene            290..709
                     /locus_tag="VGB16_00860"
     CDS             290..709
                     /locus_tag="VGB16_00860"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_014855067.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="cobalamin B12-binding domain-containing protein"
                     /protein_id="HEX9916259.1"
                     /translation="MERKIRILLAKPGLDGHDRGIKVIAAAFRDAGFEVIYTGLRQTA
                     EMIVEAAIQEDVDAIGMSILSGAHMTLFPRVLELLREKGADHILLFGGGIIPKEDIEK
                     LEKIGVGNLFTPGATTTEAIEYVKKAVSERRKEEKIF"
     gene            969..1226
                     /locus_tag="VGB16_00865"
     CDS             969..1226
                     /locus_tag="VGB16_00865"
                     /inference="COORDINATES: protein motif:HMM:NF024940.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="helix-turn-helix domain-containing protein"
                     /protein_id="HEX9916260.1"
                     /translation="MMRVLNLTMQEAERYKVISEVEGGYLKVKEAAGILGLSERQVYR
                     IKARVEKEGAGGVIHKSKGKKAPLWLTERVRDKVDHFLGHS"
     gene            1347..2333
                     /locus_tag="VGB16_00870"
     CDS             1347..2333
                     /locus_tag="VGB16_00870"
                     /inference="COORDINATES: protein motif:HMM:NF013753.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="transposase"
                     /protein_id="HEX9916261.1"
                     /translation="MPAYSIRKWLSIPQYKITRIIGDDDKEIHVWLEPYQRKAFVCSG
                     CGEVHEAGYHGIEESVVEDLALFEKRVYLHVVKRGYGCPKDKRIHIEEVPWVKKWGRV
                     TKRFAEKVNRLTAITTNQEAGWFWGLDDEVVYRIDKETLEEKAREKLMPPPAAINISL
                     DEVSYRKYHRYLTNVINVDERLVIWNDKGRKAEVSARGGSAYGMTGQVLCRYRRRELP
                     EDRVGGIRWSQASRQRRDATNRYAVNAVIVHDKFHLMQKLNPAFGGMDMVRKEELRKA
                     GSDKNNERIELTNCRQRFILFKNKNKLTEKQASYLLRLCEINQPIYPRQRRG"
     gene            2446..2649
                     /locus_tag="VGB16_00875"
     CDS             2446..2649
                     /locus_tag="VGB16_00875"
                     /inference="COORDINATES: protein motif:HMM:NF013753.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="transposase"
                     /protein_id="HEX9916262.1"
                     /translation="MLAQSFSPKIQYVVNRFKKKISSAISEGFNNKIKRLKRMAYGYK
                     DVEYFRLKIHQHCGLLNPRKFPH"
     gene            complement(2828..3355)
                     /locus_tag="VGB16_00880"
     CDS             complement(2828..3355)
                     /locus_tag="VGB16_00880"
                     /inference="COORDINATES: protein motif:HMM:NF021561.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF2284 domain-containing protein"
                     /protein_id="HEX9916263.1"
                     /translation="MRKYLKKTEKLGASWAKLIHPKNVVIAEWVRIKCQYGCGRYGTC
                     LTCPPFSPTPEYTRKMLEYYSKALIMIYDIYPQPKETERRQRRKMRKDIASLEREAFL
                     DGYYKAFGMASGPCNFCKACDITQPCKFEDLARPSMEACGIDVYATLAHVGYKLEVVT
                     SYDQIWHYSGLILID"
BASE COUNT         1161 a          731 c          968 g          984 t
ORIGIN      
        1 ttcaagaaga atttaatgca ggtcgtgaaa ggtcatggaa tatgatatat ggcatcaagg
       61 gtatcaatat cccctctgtt gagggaatag atatcaaact cgcctattat tacccagaag
      121 gagtatatat tggatacact atatcaaagg catattattt tcctcaatcg agatatattt
      181 tggttttcat aaatcagccc agaatggcaa gtggtttgga cacaatgcat ggttttctat
      241 tgctgaagat tgtgaaagag agtggaggtt gatagaagaa aggtgaatta tggaacgaaa
      301 aatcagaatt cttttagcca aacccggttt ggatgggcat gaccggggga ttaaggtgat
      361 cgccgccgcc tttcgggatg ccggattcga ggtgatctat acgggtctaa ggcagaccgc
      421 agagatgatt gtagaagccg ccattcaaga ggatgtggat gctatcggga tgagcattct
      481 ttccggtgcc cacatgaccc tctttcccag agtgcttgaa cttttaagag aaaagggggc
      541 agatcatatc cttctttttg gggggggaat tatccccaaa gaagacattg agaaattgga
      601 gaagataggg gtgggaaatc tttttacccc gggtgccacc actaccgaag ccatagaata
      661 tgtaaagaag gctgtctctg agagaagaaa agaagagaag attttttaaa agaaattccg
      721 aacattggtc aacggattaa gcagatgtaa cggataaact catctgttta atccgtttca
      781 tccgttctcc cgccaaaggc gggatcagga tgaatccgtt gacagtttaa cctgttggga
      841 attaaaacat tttccctaac aaccccttac ttgccacata gatcgcaccc cacgttttcc
      901 tgtcttcgca atataataat gtcagtagtt tgcaaataga gaatgtcagg ttataatcat
      961 ccctacggat gatgagggtg ttaaacctga ctatgcaaga ggcggagaga tataaagtga
     1021 tatcagaggt agaaggaggg tatcttaagg ttaaagaggc tgcagggatt ttagggttaa
     1081 gcgagcgtca ggtatatcgg attaaggcgc gagttgagaa ggaaggggca ggaggagtaa
     1141 tccataagag caagggtaag aaagctcctc tttggcttac ggagagggtc agggataaag
     1201 tagaccattt tttgggtcat tcttaaataa tgtggagaat ttttcataag ttattgatat
     1261 agagggagaa ataaaaaagc atcgactacc aaagagaaac ctgatctagt tgggtggaga
     1321 agaccaacta aatcaggagg taatcgatgc cggcatatag tataagaaaa tggctgagta
     1381 ttccacaata taaaataaca aggattatag gagatgatga taaagagatt cacgtctggt
     1441 tagagcctta ccagcggaag gcatttgttt gctccggatg tggagaagtt cacgaggccg
     1501 gatatcacgg gatagaagaa tcagtggtag aggatttggc actattcgag aagcgagtgt
     1561 atctgcatgt agttaaaaga ggatatgggt gtcctaaaga taagcggata cacattgaag
     1621 aagtgccgtg ggtaaagaaa tggggacgag taactaaacg ctttgcggag aaggtaaacc
     1681 gattaactgc cataaccacc aatcaggaag cgggctggtt ttgggggtta gatgacgaag
     1741 tggtgtatcg catagataaa gagacgttag aagaaaaagc ccgggagaaa ttgatgcccc
     1801 ctccggcagc cataaatatc agcttagatg aagtcagtta ccgtaagtac catcgctatt
     1861 taaccaacgt tataaatgta gatgaacggt tggtaatttg gaacgataaa ggcagaaagg
     1921 ctgaagtgtc cgccagaggc ggatctgcct atggcatgac cggacaggta ttatgccggt
     1981 atcggagaag agagttgccg gaggatagag tcggtggcat tagatggagc caagcatccc
     2041 gccaacggcg ggatgctacc aaccgctatg cggtaaatgc ggtgatagtg cacgataaat
     2101 ttcatctcat gcaaaagctc aatcccgcct ttggcgggat ggatatggtc aggaaagaag
     2161 agctacgtaa agccggatcg gataaaaata atgagcgaat tgaactgacc aactgtcggc
     2221 agcgatttat cttgttcaag aacaaaaata agcttacgga aaaacaagca agctatctac
     2281 taaggctttg tgagatcaac cagcctattt acccccgcca aaggcggggt taaaggaaag
     2341 ctttttacgg atttattctt accgtgatat agaagcagta aaagcatatc tccaggattg
     2401 gattaacgaa gctctttccc cgccagaggc ggggaggttt ttaagatttt agcccaaagc
     2461 tttagcccta agattcaata tgtcgtcaac cggtttaaaa agaagatcag ttcggctatc
     2521 tcagaaggat ttaacaacaa aatcaaaagg cttaaacgga tggcttatgg ttacaaggac
     2581 gtcgagtatt tcaggttgaa gattcatcaa cactgtgggt tattgaatcc aaggaaattc
     2641 ccacattaaa tgcgaaagaa ccccatcttt tgcggcggac ggcggtcggt ggactgcgga
     2701 ggtaatgtaa atattggggc tgttgccgaa ctttttgagt taaaggggca agaggtggtg
     2761 agcaaagctg aaccaaaggg tgttcgacat cataactaaa cttgtgaaac aggcttaacc
     2821 cacagagcta atctataaga atcaaaccgg aatagtgcca gatttgatcg taggatgtaa
     2881 ccacttccaa tttgtagccc acatgggcta aggtggcata gacatctatt ccgcatgctt
     2941 ccatggaggg acgagctaaa tcctcaaatt tacaaggctg ggtaatgtcg caagctttac
     3001 agaaattgca cggacccgaa gccatgccga aagctttata atatccgtcc aggaaagcct
     3061 ccctttccaa agaagctata tcttttctca ttttcctacg ctgtcttctt tcggtttctt
     3121 taggttgcgg ataaatatca tagatcatga tcaaagcttt ggaatagtat tcaagcatct
     3181 ttcgggtata ctcaggcgta ggagaaaagg gcgggcaggt cagacaggtc ccataacgtc
     3241 cgcacccata ttgacacttg atccggaccc attctgcgat caccacgttc ttagggtgaa
     3301 tcagttttgc ccagctggct cccagctttt ctgttttttt aagatatttt ctcattgctg
     3361 attctttttt agaagctcca gcgcttaact taagtgctgg tattcttaga acacagaagt
     3421 aatgcaatcg caggcagact ttgtctacac cctgtgtaat tttttcctaa aagcagtcct
     3481 caaggtgacc cagtagcggt cgaagtgttc atggttcaaa agttgacgca ccagtgggtc
     3541 gttctgatat tgacagaggt attggcagac ttctttctgt aagaagcgtc gtgcccacgg
     3601 aaaccttggc cctagcttat gcagactgtc taaggtggac actaaagtct cgacatcccc
     3661 atgattcctc ggcggtcccg ctgcctctat ctcaagaaaa tcgcaacgga cgcggttgaa
     3721 ctcatacttc ggaaggtaat actcctggcc gtgtgctaag cctatggcta tatcgtataa
     3781 tattttgata gacctcagcc tctcaggtga cagatgaggt ctcgacgtat caattagata
     3841 ggta
//
LOCUS       DASWLN010000026        12246 bp    DNA     linear   ENV 20-FEB-2024
DEFINITION  MAG TPA_asm: Candidatus Zixiibacteriota bacterium isolate
            SMAG_U2949 k141_280399, whole genome shotgun sequence.
ACCESSION   DASWLN010000026 DASWLN010000000
VERSION     DASWLN010000026.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37960594
            Sequence Read Archive: SRR10376091
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Candidatus Zixiibacteriota bacterium (soil metagenome)
  ORGANISM  Candidatus Zixiibacteriota bacterium
            Bacteria.
REFERENCE   1  (bases 1 to 12246)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 12246)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (26-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/12/2024 08:28:26
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 1,440
            CDSs (total)                      :: 1,416
            Genes (coding)                    :: 1,412
            CDSs (with protein)               :: 1,412
            Genes (RNA)                       :: 24
            rRNAs                             :: 1 (16S)
            partial rRNAs                     :: 1 (16S)
            tRNAs                             :: 21
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 4
            CDSs (without protein)            :: 4
            Pseudo Genes (ambiguous residues) :: 0 of 4
            Pseudo Genes (frameshifted)       :: 0 of 4
            Pseudo Genes (incomplete)         :: 3 of 4
            Pseudo Genes (internal stop)      :: 1 of 4
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..12246
                     /organism="Candidatus Zixiibacteriota bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_280399"
                     /isolate="SMAG_U2949"
                     /isolation_source="soil"
                     /db_xref="taxon:2053527"
                     /environmental_sample
                     /country="USA: Oregon"
                     /lat_lon="42.99 N 123.63 W"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            <1..478
                     /locus_tag="VGB16_00885"
     CDS             <1..478
                     /locus_tag="VGB16_00885"
                     /EC_number="1.-.-.-"
                     /inference="COORDINATES: protein motif:HMM:NF013434.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016491 - oxidoreductase activity
                     [Evidence IEA]"
                     /codon_start=2
                     /transl_table=11
                     /product="FAD-binding oxidoreductase"
                     /protein_id="HEX9916264.1"
                     /translation="KDLVIPTRGQILATEPVKEKLFDQKIVYADWGYEYFRQLLDGTI
                     LLGGFRQLYSDTEIGFSDETTSEIQEGLYKFLIEHFPQLNKIKITHRWAGVMGFSKDG
                     LPLVGALPQAPFVISAVGFTGHGMGFAFGLAKGLAEYLTGGKTSYPLDIFSLKRVL"
     gene            878..1093
                     /locus_tag="VGB16_00890"
     CDS             878..1093
                     /locus_tag="VGB16_00890"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEX9916265.1"
                     /translation="MKTFILMSMALATIAFLTHLFSGKMKSKPPVPANLWLFYLIEKI
                     FGENELRAKEVKENGMPQANPGQNKDV"
     gene            1050..1271
                     /locus_tag="VGB16_00895"
     CDS             1050..1271
                     /locus_tag="VGB16_00895"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEX9916266.1"
                     /translation="MECPKLIQGKIKMCDSEIFDGMVPSILELKDFCQSTEHYLCPFF
                     IGSEACASKEKVAQKLSNWNALPKQFIGA"
     gene            1440..2681
                     /locus_tag="VGB16_00900"
     CDS             1440..2681
                     /locus_tag="VGB16_00900"
                     /inference="COORDINATES: protein motif:HMM:NF019042.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="porin"
                     /protein_id="HEX9916267.1"
                     /translation="MKARIFLILILLVILGVSTSWAQTQEDSTEAAMQELKDRLDGIS
                     ENISTLNSDVGILKYIKVTGYIQARYEYNDSSKYGANLNANNFYIRRGRIKLTVQPGT
                     SSKYVIYFDASKNTVSLKEAYIELYKAVQQSNFTLTFGQFNWPFGYEIEYSSSKRDFP
                     ERSLAENNLFKGERDRGANLTLALPKYLQLNLGIFQGYGIENSTFAWADPTKPKDVIA
                     RAKTKLGMLDFGVSGYWGKNHTSGSAAVAGTTTWYDADGDAVVDPGEIKTTAPKAAVL
                     AREDDKIRYGADAQLYLDFLPIGGTGIRAEYYQAKDYNSSAKKVANEKGWYLWLSQSI
                     TNKFGAATRYDYWNPNTDTNVKNDATGTLSLAMHYFWDSYVRITAAYDIPHKLKDNSI
                     FSKYSGDVKDNRFTLQFQFTY"
     gene            2748..3557
                     /locus_tag="VGB16_00905"
     CDS             2748..3557
                     /locus_tag="VGB16_00905"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_008589291.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0055052 - ATP-binding cassette (ABC)
                     transporter complex, substrate-binding subunit-containing
                     [Evidence IEA];
                     GO_function: GO:0042626 - ATPase-coupled transmembrane
                     transporter activity [Evidence IEA];
                     GO_function: GO:0140359 - ABC-type transporter activity
                     [Evidence IEA];
                     GO_process: GO:0006817 - phosphate ion transport [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="phosphate ABC transporter substrate-binding
                     protein"
                     /protein_id="HEX9916268.1"
                     /translation="MGISLFLAAYFAAYAGKAITVKGSDTMLILGQRWAEIYMNQNPG
                     VTIQVTGGGSGVGIAALINGSTDICEASRSMKSSEIDKLKERFNTIGVEIPVARDGLS
                     IYLNAENKISELTLDQLRDIYTGKTTNWKDLGGEDTKIVLYGRENSSGTYSYFKDNVL
                     QGLDFAAQTQTLPGTGAIVNAVSKDKFGIGYGGAAYAKAVKYSKVKKDATTPGYEPTL
                     ENIKNGKYPISRYLYWYLRNKPTGEIKKFVDFVLSPQGQEIVTKVGYFPVK"
     gene            3611..4573
                     /gene="pstC"
                     /locus_tag="VGB16_00910"
     CDS             3611..4573
                     /gene="pstC"
                     /locus_tag="VGB16_00910"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011984740.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0005886 - plasma membrane [Evidence IEA];
                     GO_component: GO:0055052 - ATP-binding cassette (ABC)
                     transporter complex, substrate-binding subunit-containing
                     [Evidence IEA];
                     GO_function: GO:0015415 - ATPase-coupled phosphate ion
                     transmembrane transporter activity [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="phosphate ABC transporter permease subunit PstC"
                     /protein_id="HEX9916269.1"
                     /translation="MKRLGTLEKNLQAKPLGFKKRFRLSEFLIEKAIFISSFFAILGV
                     ILIFLFVFREALPIFTNPEVTKEASLRLFFSTTIWQPISDNPRYGLIPIIVGTLKVVV
                     VALFFSVPISVAAAIYTSEFAPLRAKEYIKPVVELLAGIPSVVLGFFALMVMASFFHG
                     IFHSTMRLNALNAGVALSFAIIPVVYSIAEDSLNAVPRSIKEASLALGANRWQTAFRV
                     SLSVAMPGVLAGVILGLGRAVGETMIVLMASGNASILSGNIFDSVRTMSATIAAELGE
                     VVHGSAHYNVLFFIGASLFIFTLFINSLAHLTVTRLQRKLAGKI"
     gene            4574..5485
                     /gene="pstA"
                     /locus_tag="VGB16_00915"
     CDS             4574..5485
                     /gene="pstA"
                     /locus_tag="VGB16_00915"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_007416654.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0005886 - plasma membrane [Evidence IEA];
                     GO_component: GO:0055052 - ATP-binding cassette (ABC)
                     transporter complex, substrate-binding subunit-containing
                     [Evidence IEA];
                     GO_function: GO:0015415 - ATPase-coupled phosphate ion
                     transmembrane transporter activity [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="phosphate ABC transporter permease PstA"
                     /protein_id="HEX9916270.1"
                     /translation="MNSKIKTNIISSFFTGLTLLATLIIFTILIIIIGNIIIYGHKTI
                     SWEFITKAPREGMTAGGIFPAIFGTVALVVLMIIAVLPVGVMTAIYLHEYSKPDFLLT
                     KIIRFAVNNLAGVPSIVFGLFGLGFFIQFVGAGIDKFFGLEVIWGQPCLMWAALTLAL
                     LNLPVVIVASEEALRAIPKETREASLALGATKWQTIRRVVLPQATPGILTGAILSISR
                     GAGEVAPIMFTGAAYFLPYLPHKPTDQFMELGYHIYVMATQSPDVETTKPILYGTVLT
                     LLILTLLLNFVAIFIRSKIRRQMKKSF"
     gene            5518..6318
                     /gene="pstB"
                     /locus_tag="VGB16_00920"
     CDS             5518..6318
                     /gene="pstB"
                     /locus_tag="VGB16_00920"
                     /EC_number="7.3.2.1"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_014560754.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0009898 - cytoplasmic side of plasma
                     membrane [Evidence IEA];
                     GO_component: GO:0055052 - ATP-binding cassette (ABC)
                     transporter complex, substrate-binding subunit-containing
                     [Evidence IEA];
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_function: GO:0015415 - ATPase-coupled phosphate ion
                     transmembrane transporter activity [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="phosphate ABC transporter ATP-binding protein
                     PstB"
                     /protein_id="HEX9916271.1"
                     /translation="MDNRITEISQNRLEEFSSKILVEGLNFYYGRNHALKDVSVNIKA
                     NQVTAFIGPSGCGKSTFLRCLNRMNDLIPGVKVSGKVYFDGMDIYSKDIGLAELRRKV
                     GMVFQKSNPFPKSIFENVAYGLRIQGITNKSHLEETVEKTLRDAALWDEVKDKLDESA
                     MKLSGGQQQRVCIARALAVEPEVLLMDEPASALDPISTAKIEELILDLKKNYTIVIVT
                     HNMQQAARISDYTGFFFLGELIEFDDTSKIFTNPSNKKTEDYVTGRFG"
     gene            6339..7028
                     /gene="phoU"
                     /locus_tag="VGB16_00925"
     CDS             6339..7028
                     /gene="phoU"
                     /locus_tag="VGB16_00925"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_012647093.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003700 - DNA-binding transcription factor
                     activity [Evidence IEA];
                     GO_process: GO:0051049 - regulation of transport [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="phosphate signaling complex protein PhoU"
                     /protein_id="HEX9916272.1"
                     /translation="MTISRHLDKDLNLLKEKLLLMASLAESMIHKAVKSLVEREQSYF
                     KQVKEEENKVNHLEIEIDDLCLKLLALKQPMATDLRFITSAMKINSNLERIGDLAINI
                     TERAAVLLKQPQLKPLIDIPRMADMAQKMVKDSLDAFIKKDAGLARSVLERDDQIDGL
                     KDQIFRELLTFMLSDQSTIPRALELILVSRHLERIGDHATNIAEDVIYLVQGKDIRHH
                     IEEEERDHNKS"
     gene            7046..7732
                     /locus_tag="VGB16_00930"
     CDS             7046..7732
                     /locus_tag="VGB16_00930"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_018247522.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003677 - DNA binding [Evidence IEA];
                     GO_process: GO:0000160 - phosphorelay signal transduction
                     system [Evidence IEA];
                     GO_process: GO:0006355 - regulation of DNA-templated
                     transcription [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="response regulator transcription factor"
                     /protein_id="HEX9916273.1"
                     /translation="MSKQILVIEDDKDIVQLLKHYLEKEGFVVKEAADGFSGLEKTKS
                     NKFDLIILDIMLPEMDGLEVCKELRSDPKTASIPIIMLTAKTEETDKIVGLELGADDY
                     VTKPFSPKELIARIKAIFRRVGRKSEKEKAYSYGKLVLDISKHEVVFDSRPIELTSKE
                     FDLLESLLQNKGRVLSRDYLMDEVWGYDYYGGMRTVDVHIRRLREKIPFLSKSIQTIK
                     NMGYKLKENP"
     gene            7779..9545
                     /locus_tag="VGB16_00935"
     CDS             7779..9545
                     /locus_tag="VGB16_00935"
                     /inference="COORDINATES: protein motif:HMM:NF028046.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ATP-binding protein"
                     /protein_id="HEX9916274.1"
                     /translation="MKLRLKLFLTYLLLSLLCLFIAGILIVLSEQRYSRAELERNMLF
                     ETELLSKLFVPVLTNNMSQTKIDSLTKELGSKIEGRITVINKTGKVLGDSYESEKDVP
                     NMENHINRPEVARALRGDVGKIVRFSYTIKTDMLYVACPIKDDTGIIGVVRLAFPIIQ
                     LRQYQNRIMDIVLIGLLIALAFSLLLSFVFSSKITKPLRQMMRVSKKISQGDFTQKVK
                     VKTKDEIGELSQILDQMSLELSGKIDQINEDKSRLDSILSNMTEGVLALDHRGKVVLA
                     NDALSRMFELDAVYGRLYYEAIRDYALNQFIQEVLSSKQAKNKEISFFHPQEKVFMVQ
                     STLVGSQKEDTVFAIFVFYDITELKKTEKIRKDFVANVSHELKTPLTSIKGFVEALQD
                     GAINNTQQANYFLSIISQHTERMNKIISDLLQLSLIEAEEFQLKIEPFAIKEVIEGVA
                     SMLKNSAEKKSQNIEMKLYSEEQRVLADKYRINQALTNLVENAIRYTPEKGTIKIQTE
                     DKGDLIEIAVMDNGIGIPSKDLPRIFERFYTVDKGRSRESGGTGLGLSIVKHIIEAHN
                     GKIHVESKSGKGSKFSFDLKKA"
     gene            complement(9712..10263)
                     /locus_tag="VGB16_00940"
     CDS             complement(9712..10263)
                     /locus_tag="VGB16_00940"
                     /inference="COORDINATES: protein motif:HMM:NF012903.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005198 - structural molecule activity
                     [Evidence IEA];
                     GO_process: GO:0071973 - bacterial-type
                     flagellum-dependent cell motility [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="flagellin"
                     /protein_id="HEX9916275.1"
                     /translation="MLKLQTADGYLSEVNNQLIEMKRIIIQAANTGLNDPAQISALQK
                     GMKEIAESTNQMIPNAQFVSNNLFDGLSGSVANIGSMANFDITTQAGAEKTLSQIEER
                     INQISEIRGKLGGEYKYTLETNRNNLMVSLQNLMSAESSIRDVDMAKEYVDFVKNKLN
                     LEAGMAMLAHGSLSSKNIISLLK"
     gene            11160..>12246
                     /locus_tag="VGB16_00945"
     CDS             11160..>12246
                     /locus_tag="VGB16_00945"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_013406466.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_function: GO:0016887 - ATP hydrolysis activity
                     [Evidence IEA];
                     GO_function: GO:0042626 - ATPase-coupled transmembrane
                     transporter activity [Evidence IEA];
                     GO_function: GO:0140359 - ABC-type transporter activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="ABC transporter ATP-binding protein"
                     /protein_id="HEX9916276.1"
                     /translation="MAKVELKNVSKFFNKDNLVVKDINLDIRDGEFFTIVGPSGCGKS
                     TILNMIAGLEEVTHGEILFDDQIVNNIPPAKRNVAMVFQSYALYPHKNVFENIAFPLR
                     TRKVNRRDVENRVKEVAGILGLENLLHKKPKELSGGQRQRVALGRAIVRKPSIFLLDE
                     PLSNLDAKLRVYMRAELKRLHRQLKITTIYVTHDQAEAMTLSDRVALFFEGRIQQCDK
                     PEVLYNNPANRIVAEFIGSPAMNLIQAKISEKKGQMETIFDSGEKLFLPEELKFKPKD
                     DSVLFGIRPEDVEVSLNFKEGYFKGELFAIEPTGSSTYLDILWNGNRLKAEVSPDFKA
                     NIDQNIYFLFNLNRCHLFDLKSGENIKG"
BASE COUNT         3726 a         2364 c         2666 g         3490 t
ORIGIN      
        1 taaagacttg gttataccaa ctcgcgggca gattctggca actgagccgg tcaaagaaaa
       61 actcttcgac cagaagattg tctatgcgga ctggggatac gaatatttca gacagctttt
      121 ggatggaact atacttctgg gcggattcag acaactttat tctgatactg aaatcggctt
      181 cagtgatgag acgacttcag aaatacaaga gggtttatat aaatttctaa ttgagcattt
      241 ccctcaattg aataagataa agataactca ccgctgggct ggagtaatgg ggttttccaa
      301 ggatggactg cccttagtgg gagcgcttcc ccaagcccct tttgtcatca gtgcagttgg
      361 attcaccggg catggaatgg gttttgcctt tggcttggca aaggggttgg cggaatatct
      421 aactggaggg aaaacttctt atccgttaga tattttttca ttaaaaaggg tattgtagga
      481 acctttcccc ttctcttata cctaccgaat tcatcggcca cgttcaaatt agggcgggaa
      541 cgtagatttc tttcctctat gcacatacct tttcctttca gttatagaaa ttgcgccatt
      601 cgattcttca tcaagtccag tttttccatc acccacaaag tccttttcat aatctagtct
      661 ctgatattat ttaaccacca gttaatactg agttaatacc aaagaaatag tgacttgttt
      721 gttttagagg agaaatagag atcatggaaa tgggccataa tcttaacctc gatttaacct
      781 gcggttaatc tcatcgtaat caaacaatcg tttactttaa gtgagtttga aaaggcttct
      841 cgaataagaa aacagttcgc atgggagttt aaaaaatatg aagacgttca ttctaatgag
      901 catggcactt gccactattg cttttttgac tcacctgttt tcaggaaaaa tgaaaagcaa
      961 accaccagtc ccggcaaatt tatggctctt ttacttaata gagaagatat ttggcgaaaa
     1021 tgagttacga gcaaaggagg tgaaagaaaa tggaatgccc caagctaatc cagggcaaaa
     1081 taaagatgtg tgactcagag atattcgatg gaatggtgcc cagtattctc gagctcaaag
     1141 atttctgtca aagtacggaa cactacctct gtccgttttt tattggttcg gaagcatgcg
     1201 caagtaaaga gaaggtcgct caaaagcttt ccaactggaa tgctttaccc aaacagttta
     1261 tcggagctta agatgtaagg aatgaaaaca gggcataaat ttaacttgta attaatactt
     1321 acttaacacg gatgaaacat tggtagacgt ctatacaata aagtagtaaa ggaggtgaaa
     1381 tgaaaacttg agcgaacaat aaaaaacaac caaaaacctt caaacaaagg aatgaggaaa
     1441 tgaaagcaag aatatttttg attttgatat tactggttat cttgggtgtg agcacatctt
     1501 gggctcaaac ccaagaagac agtaccgagg cagcaatgca agaactaaaa gaccgtctgg
     1561 atggaatttc tgaaaatata tcaactttga atagcgatgt aggaattctg aaatacataa
     1621 aagtaaccgg ctacatccaa gccagatatg agtacaatga cagctccaaa tatggcgcca
     1681 acttgaatgc caataacttc tatattcgtc gtggaagaat aaagctcacg gttcagcccg
     1741 gaacttcaag caagtacgtt atctacttcg atgcttccaa gaacacggtt tctttaaaag
     1801 aggcctatat tgaattgtac aaagctgtgc aacaaagtaa cttcactttg accttcgggc
     1861 aatttaactg gccctttgga tatgaaattg aatactcttc ttccaaaaga gattttccgg
     1921 aaagaagttt agcggagaat aatcttttca agggagaaag agaccgggga gccaatttaa
     1981 ctctagctct tcccaaatat ctacaactga atttaggcat ttttcagggt tatggaatag
     2041 aaaatagcac tttcgcctgg gctgacccca ctaagccgaa ggatgttatt gcccgagcta
     2101 aaacaaaact gggaatgctg gacttcggag tttccggcta ttggggtaaa aaccacacct
     2161 ctggaagtgc agctgttgcc ggaacaacca cctggtatga tgcagatggg gatgcagtag
     2221 tggatccggg tgaaatcaag accactgctc ccaaagctgc tgtcctggca agggaagatg
     2281 ataagataag atacggagct gatgctcaac tttatcttga cttcttgccg attggcggca
     2341 ctgggatacg ggctgaatac tatcaagcca aggactataa cagcagtgcg aagaaggttg
     2401 caaatgaaaa aggttggtat ttgtggttat cccagagcat caccaataag tttggtgctg
     2461 cgacaaggta tgattactgg aaccccaata cggatactaa cgtaaaaaac gacgctactg
     2521 gaacattatc tttggcaatg cattacttct gggattccta tgttagaatc acagctgctt
     2581 atgatatacc tcataagctt aaggacaatt cgattttctc taagtattct ggtgatgtaa
     2641 aagataacag atttaccttg caatttcagt tcacctacta aatatttaac taattaaagg
     2701 aggttaaaat gaagagatcg tttcttaaga aattgggttt ggcaattatg ggaatctcgc
     2761 tttttctggc tgcatatttt gcggcttatg ccggaaaagc tataactgtt aaaggttccg
     2821 atactatgct tattctgggg cagagatggg cggagattta tatgaaccag aatcctgggg
     2881 tgactatcca agttaccggt ggaggttcag gtgtaggaat cgccgcattg attaatggtt
     2941 caactgacat ctgtgaagct tcccggtcga tgaaaagctc ggaaatcgat aaattaaaag
     3001 aacggttcaa taccatcggg gttgaaattc cggtggcaag agatgggcta tcgatttatc
     3061 ttaatgcaga gaacaaaata tccgagctta ctttggacca acttagagac atctataccg
     3121 gcaagacaac caactggaag gacctgggtg gggaggatac caagatagtg ctttatggca
     3181 gagaaaatag ctccggtact tatagctatt ttaaagacaa cgtcttgcag ggtttagatt
     3241 ttgcggcgca gacccagacc cttccgggaa ctggagctat tgttaatgct gtgtctaaag
     3301 acaaatttgg aatcggctat ggtggggctg cctacgccaa ggcagtcaaa tactctaaag
     3361 tcaaaaagga cgcaacaacc ccgggttatg aaccgacttt ggagaatatc aagaacggca
     3421 aataccccat ctctcgatat ctgtactggt atttaaggaa taaacccaca ggagaaatca
     3481 agaaattcgt ggattttgtc ctgtcgccac aggggcagga gattgtgacc aaagttggat
     3541 actttccagt gaagtagatt ttatgtaggg gcgacccgat gggtcgcccc tacaacccga
     3601 tttctaattt atgaaaagac tagggacact tgagaaaaat cttcaggcga aacctcttgg
     3661 ttttaaaaag aggtttcgcc tttcagaatt tcttatcgag aaagccatct ttatttcctc
     3721 tttctttgcc atcttgggcg tgattcttat ctttttattt gttttccgtg aagccctgcc
     3781 catctttacc aatcccgaag tcacaaaaga ggcaagttta agacttttct tttccactac
     3841 tatctggcag ccaatctcgg ataatccaag atacggcttg attcctataa tcgtgggtac
     3901 cttaaaggta gtggtggtcg ctcttttttt ctctgttccc atttctgtgg cggcagccat
     3961 ttacacttca gaatttgctc cgcttagggc taaggaatat atcaagccgg tagtcgaact
     4021 tttggcagga atcccgtcag tggttttggg tttttttgct ttgatggtga tggcttcatt
     4081 ttttcacggc atatttcatt ccaccatgcg cttgaatgcc ttaaatgccg gtgtggcgtt
     4141 aagctttgcc attattccag tagtttattc catagctgaa gactctctaa atgctgttcc
     4201 cagaagcata aaggaagctt ctttagcttt gggagcaaat cgctggcaga cggctttccg
     4261 agtatctcta tctgtggcta tgcctggtgt tttggctggg gtaattttgg gattgggtcg
     4321 ggctgtggga gagacaatga ttgttttgat ggcttcgggc aatgcctcga ttttgtctgg
     4381 aaatatcttt gattctgttc gtacaatgtc ggctactatt gctgcggaat taggagaagt
     4441 tgtccatgga agcgcgcact acaatgtcct gttttttatc ggcgcttccc tttttatttt
     4501 cactttgttt ataaattcgc tggctcacct gaccgttacc agattgcaaa gaaaattagc
     4561 tgggaaaatt tagatgaaca gcaaaatcaa aactaacata atcagttcct ttttcaccgg
     4621 cttaaccctc cttgccacct taattatatt taccattctg attataatca tcggaaacat
     4681 cataatatat ggacataaga ccataagctg ggagtttatc actaaagctc cacgggaagg
     4741 aatgaccgca ggtggcattt tcccggctat tttcggaacc gtggctttag ttgtcctgat
     4801 gatcatcgca gttcttccgg tgggagtgat gaccgcaatt tatctacacg aatactccaa
     4861 gcccgatttt ctcttaacca aaattatccg ttttgccgtg aataatttgg ctggcgtacc
     4921 ctcgattgtg tttggactgt tcggcttggg ttttttcatt caattcgtcg gagcaggaat
     4981 agataaattt ttcggtctgg aagtaatctg ggggcaaccc tgccttatgt gggcggcttt
     5041 gactttggct cttttgaacc ttccggtagt tatagtggcg tcagaagaag ccttaagggc
     5101 aattcccaaa gagaccagag aggcgtcttt agctttgggc gcaaccaaat ggcagactat
     5161 tcgtcgggta gtgcttcctc aagctactcc cggaatttta accggggcta tactttccat
     5221 tagtcgtggc gcaggagaag ttgctcctat aatgttcacc ggtgcagctt attttctgcc
     5281 ctatcttccg cataagccga ccgaccaatt tatggaattg ggttatcata tctatgttat
     5341 ggcaactcag tctccggatg tggaaaccac taagcccata ctttacggaa cagtcctgac
     5401 actcttaatt ttgactttgc ttttgaactt tgttgctata tttataaggt ctaaaatcag
     5461 aagacagatg aagaaaagct tttaaatagc ctcaagaaga tggatgaaaa agcaaaaatg
     5521 gacaatcgaa ttactgaaat ctcacaaaac agactagaag agttctcttc caagattttg
     5581 gttgaagggc tgaatttcta ctatggcaga aatcatgccc taaaagatgt ctccgtcaac
     5641 ataaaagcca atcaggtaac cgccttcatt ggcccttccg gctgcggtaa gtcaaccttt
     5701 ttaaggtgtc ttaaccggat gaacgatttg attccaggag ttaaagtttc aggcaaggtt
     5761 tactttgatg gaatggatat atattcaaag gatattggcc tggctgaatt aagaagaaaa
     5821 gttggaatgg tttttcaaaa atccaatcct ttccccaaat ccattttcga aaatgtagct
     5881 tatggcttaa gaattcaggg cattactaat aaatcccatc tggaagaaac tgtggagaaa
     5941 accttgaggg atgctgcttt gtgggatgag gtgaaagata agctggatga atccgcaatg
     6001 aagctctccg gcgggcaaca gcaaagagta tgcattgccc gagcattagc cgtagaaccg
     6061 gaggttttgc ttatggatga gccggcatcc gctttggacc caatctcgac tgccaaaatc
     6121 gaggaattaa tccttgactt gaagaaaaac tatactattg taattgtaac ccataatatg
     6181 cagcaggccg cccgaatttc cgattacacc ggatttttct ttctgggaga actgatagaa
     6241 tttgacgata cttccaaaat atttaccaac ccgtccaaca agaagaccga ggattatgta
     6301 accggtcgtt ttggataaga acctaaaaag gaaaaaatat gacaatctcc aggcatttag
     6361 ataaagattt aaacctgctt aaagaaaagc ttcttcttat ggccagttta gccgaatcga
     6421 tgattcataa ggcggtgaaa tctctcgtgg aaagggagca gtcctatttc aagcaggtga
     6481 aagaggagga gaataaggtc aatcatttgg aaatagagat cgacgacctt tgcttgaagc
     6541 ttttagcatt gaaacagcca atggcaacgg atttgagatt tatcacttca gctatgaaaa
     6601 tcaacagcaa cttggagagg attggcgatt tggcaataaa catcaccgag cgggctgcgg
     6661 ttctgctgaa gcaacctcaa cttaaacctt taatcgatat accccgaatg gcagatatgg
     6721 ctcagaagat ggttaaagat agtctggatg cctttataaa gaaggatgcg ggtttagctc
     6781 gttctgtttt ggagcgggat gaccaaatcg acggattgaa agaccagatc ttccgagagc
     6841 ttttaacttt tatgctcagc gaccaatcca caatcccgcg ggctttggag ctgattttgg
     6901 tctccaggca cttagaaaga ataggtgatc acgctaccaa tatagctgaa gatgttatct
     6961 acttagtgca gggcaaggat atcaggcatc atatcgaaga agaagaaaga gatcacaata
     7021 agtcatagaa tgttttgaaa taattatgag taaacaaatc ttagtgattg aggacgataa
     7081 agatattgtt cagttgctca aacattattt ggaaaaagaa ggttttgttg taaaagaagc
     7141 ggctgatggt ttttcgggtt tggaaaaaac gaagtcgaat aaatttgact taataattct
     7201 ggacataatg cttccggaaa tggatgggct tgaagtctgc aaggaattga gaagtgaccc
     7261 aaaaactgct tcaatcccta taataatgct gaccgctaag acagaggaga cagataaaat
     7321 agttggtttg gaattgggcg cggatgacta tgtaaccaag cccttcagcc caaaagaact
     7381 tatcgccaga ataaaagcca tatttcgacg agttggaaga aaatcggaga aagaaaaagc
     7441 ttattcctac ggaaagctcg tcttagatat ctcaaaacat gaagttgttt ttgacagtcg
     7501 tccgattgaa ttaacttcaa aagaatttga cttgctcgaa tctcttttgc aaaacaaagg
     7561 aagagtatta agcagagact atttgatgga cgaggtctgg ggctatgatt attacggagg
     7621 aatgcgcacc gtggatgtcc atatcagaag attaagagag aaaatacctt tcttatccaa
     7681 gagcattcag accataaaaa atatgggcta caaacttaaa gagaatcctt gaggataaat
     7741 attcagtcta atgtttctat tcgcgagatt ctgataatat gaaactgcgt ctaaaactat
     7801 ttctcactta ccttctgctt agccttttgt gcctatttat tgccggaatt ctgattgtat
     7861 tatccgaaca gagatattca cgggctgagc tcgaaaggaa tatgcttttt gagaccgaac
     7921 tgctttccaa acttttcgtt cctgtcttaa ctaataacat gagtcagacc aaaatagata
     7981 gcttgaccaa agagcttggc agcaaaattg agggacgaat cactgtcata aacaagaccg
     8041 gcaaggttct gggtgattcc tatgaaagcg aaaaagacgt cccgaatatg gaaaaccaca
     8101 ttaaccgacc ggaagtggca agggctttgc ggggtgacgt agggaaaatt gtgcgcttta
     8161 gctatacaat taaaacagat atgctctacg ttgcctgtcc cataaaagac gacaccggaa
     8221 taatcggagt agtgcgcctt gcgtttccta tcattcagct taggcaatat cagaacagga
     8281 ttatggatat tgtcctaatt gggcttttga tagctttggc tttttctctg cttttgagtt
     8341 tcgtattttc ctctaagata acaaaaccct taaggcaaat gatgcgggta agcaaaaaaa
     8401 tatcccaagg ggactttacc caaaaagtaa aagtaaaaac caaagatgaa ataggtgaat
     8461 tgagtcagat cttagaccaa atgtctttag agctttcagg taaaatcgac cagataaatg
     8521 aggataagtc aagattggat tccatcttat ctaatatgac agaaggcgta ttggcacttg
     8581 atcatcgagg aaaagttgtc ttggcgaacg atgcccttag tcgtatgttc gaactggatg
     8641 ccgtttatgg gagactctat tatgaagcta tccgagatta tgccttgaat caattcatcc
     8701 aagaggttct ttcaagtaaa caggcgaaaa acaaagaaat ctcctttttt cacccacagg
     8761 agaaagtttt tatggtgcaa tcgactttag tcggttcgca aaaggaggat acggtttttg
     8821 caatattcgt tttctatgat atcacagagc ttaaaaaaac tgaaaaaata agaaaggatt
     8881 ttgtggctaa tgtttcacac gagctaaaaa cccctttgac ctcaatcaag ggttttgttg
     8941 aagcgcttca ggatggagca ataaataaca cacaacaggc aaattatttc ctttccataa
     9001 tttcccagca taccgagcgg atgaataaaa tcatctccga cctgctgcaa ctttctctga
     9061 ttgaagccga agaattccag ttgaaaatag agccatttgc gattaaggaa gttatcgaag
     9121 gtgttgcatc tatgcttaaa aactcggcgg agaagaaatc gcaaaacata gagatgaagc
     9181 tttattctga agagcaaaga gtgttggcgg ataaatatag aataaatcag gccttaacta
     9241 accttgtgga aaatgctatt agatatactc cggagaaagg cacaataaaa atccagactg
     9301 aagataaagg ggatttaatc gaaatagcag taatggataa tgggattggt attccctcga
     9361 aagacctgcc tcgcattttc gaaagatttt atactgtgga caaaggtcgc tccagagaat
     9421 ccggaggcac gggattaggg ctttccatag tcaagcacat aattgaagcc cataatggca
     9481 aaatccatgt ggaaagcaaa tcgggaaagg gaagcaagtt cagttttgat ttgaagaagg
     9541 cataaaacaa aaatattaag ctaagtcaaa gcttgttgct tcctatctat cggtaacata
     9601 gcctgtcaca tgtgagcttg tgcaaatggg ttctggatga tcccaaagga aatagaattt
     9661 gggcgttccg ctatcgaaag cgggtgagtg gccggtgctg gaaaacatat ctcatttcaa
     9721 aagcgaaata atattcttcg agctcaaact cccgtgagcc agcatcgcca tcccggcttc
     9781 taagttcagc ttgttcttta cgaaatcaac atattccttt gccatatcca catctcggat
     9841 ggatgattcc gcactcatca aattttgtaa actaaccatc aagttattgc gatttgtttc
     9901 cagtgtatat ttatactccc cacccaactt cccccgaatc tccgaaatct ggttgatcct
     9961 ttcttctatc tgggatagag tcttttctgc tcctgcctga gtcgtaatgt cgaaatttgc
    10021 catggaacca atatttgcta cacttccgct taaaccatcg aaaagattat tactaacgaa
    10081 ctgagcattt ggaatcatct gattggtgct ctccgctatt tctttcatgc ctttttgtag
    10141 cgctgaaatc tgagcagggt catttaagcc agtatttgcc gcctggataa taatcctttt
    10201 catctctata agctgattat tgacctcact taaatatcca tctgcggtct gaagtttcag
    10261 catagagtac tcctcccttc aatctctttt tccaagccac ctatctggga acgtatcttt
    10321 gcggaaatga caagtccagc cgggtcatcc gaagcctgat ttattcttca accactagag
    10381 agttgttgaa cattcttgct taagttcctg gttatccgaa aaagttccga caaaaccctc
    10441 tcttcatttt gaaaggaatt tatccttaac acctttcccc ctttgcgagc tttacatcaa
    10501 gtcattctag taaaaacgat gcctaccgga ccaaaaacac ccctcaaatt ggcggaagta
    10561 agcgcttgtc aattcacaag ttgtatcgta tcgaaaaatg gatgagacag actgacgata
    10621 aactgaattt atctattgtt gttctgttat actctattgg atttatccta tattgagatt
    10681 tttattggtt tctgaaaaac tattttaaaa gggtactttg gagtggtcac cttccttcgg
    10741 caagctcagg acggtgagca aagtcgaacc gttaggtgaa ccaagttaaa gctttttgtt
    10801 tttcaacagg tttcgggtca agctaaagct tgacactcca tagaaacctt ttccaattcg
    10861 actttttcaa aagacctctt tttaagagct tgacaactta agcatatcct ttagattgaa
    10921 agtagtatta tatttgtcat tcccgcgaat gcaggaatcc atcgggtttt caatcaaaac
    10981 ttgattccgc atcaagtgcg gaatgacaag caaaatcact ttctaaaaat aacagacaag
    11041 ctgtagcagt ttgattcgtc gaacgatttt gtttttatcg tagttgcccg attcattggg
    11101 cgaggtttga attcgctcga taaatcgagc ccctacaaaa tttctaaact taattaatta
    11161 tggcgaaagt ggaactaaag aatgtaagta aatttttcaa caaagacaat ttagtagtta
    11221 aggatattaa tttggacata cgagatggcg aattctttac catcgttgga ccttctggtt
    11281 gtggaaagtc aactatcttg aatatgatcg ctggattgga agaggttacg catggtgaaa
    11341 tactgtttga tgaccagata gtaaataata ttccccctgc gaaaagaaat gtggctatgg
    11401 ttttccagag ttacgctctt tatccgcaca agaatgtgtt tgagaatata gctttcccct
    11461 tgaggacaag aaaggtaaat cgtcgggatg tagaaaatag agttaaagag gtagctggga
    11521 ttttgggatt ggagaactta ctgcataaaa aacccaaaga gttgagcgga ggacaaaggc
    11581 agagagtggc tttgggacgt gccatagtca gaaaaccaag tatcttcctg ttagatgagc
    11641 ccctttccaa tctggatgcc aaactgcgtg tctatatgcg ggctgagctc aaaagactac
    11701 atcgtcagtt aaaaataaca actatctatg tcacccatga ccaagctgaa gctatgaccc
    11761 tctcggacag ggtggctttg ttttttgagg gcagaattca acagtgtgac aagccagagg
    11821 ttttgtataa taatccggct aaccgcatcg tagctgaatt tatcggaagc ccggctatga
    11881 atttaattca ggcaaaaata tcagagaaaa aagggcagat ggaaaccata ttcgattcag
    11941 gtgaaaaatt atttcttccg gaggaattga agtttaaacc aaaagatgat tctgttctat
    12001 ttggaatcag accggaggat gtggaggtaa gtttaaattt caaagaaggg tatttcaaag
    12061 gtgagctttt cgccatagag ccaacaggaa gtagcaccta cctggatatt ctgtggaatg
    12121 gaaaccgtct caaggcagag gtttcgcctg acttcaaggc aaatatagac cagaacatct
    12181 atttcttatt caatctcaat cgttgtcatc tgtttgacct aaaaagcggg gaaaacatta
    12241 agggct
//
LOCUS       DASWLN010000027         2458 bp    DNA     linear   ENV 20-FEB-2024
DEFINITION  MAG TPA_asm: Candidatus Zixiibacteriota bacterium isolate
            SMAG_U2949 k141_281376, whole genome shotgun sequence.
ACCESSION   DASWLN010000027 DASWLN010000000
VERSION     DASWLN010000027.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37960594
            Sequence Read Archive: SRR10376091
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Candidatus Zixiibacteriota bacterium (soil metagenome)
  ORGANISM  Candidatus Zixiibacteriota bacterium
            Bacteria.
REFERENCE   1  (bases 1 to 2458)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 2458)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (26-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/12/2024 08:28:26
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 1,440
            CDSs (total)                      :: 1,416
            Genes (coding)                    :: 1,412
            CDSs (with protein)               :: 1,412
            Genes (RNA)                       :: 24
            rRNAs                             :: 1 (16S)
            partial rRNAs                     :: 1 (16S)
            tRNAs                             :: 21
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 4
            CDSs (without protein)            :: 4
            Pseudo Genes (ambiguous residues) :: 0 of 4
            Pseudo Genes (frameshifted)       :: 0 of 4
            Pseudo Genes (incomplete)         :: 3 of 4
            Pseudo Genes (internal stop)      :: 1 of 4
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2458
                     /organism="Candidatus Zixiibacteriota bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_281376"
                     /isolate="SMAG_U2949"
                     /isolation_source="soil"
                     /db_xref="taxon:2053527"
                     /environmental_sample
                     /country="USA: Oregon"
                     /lat_lon="42.99 N 123.63 W"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            <1..875
                     /locus_tag="VGB16_00950"
     CDS             <1..875
                     /locus_tag="VGB16_00950"
                     /inference="COORDINATES: protein motif:HMM:NF014062.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=3
                     /transl_table=11
                     /product="NfeD family protein"
                     /protein_id="HEX9916277.1"
                     /translation="LGKVENDAAAYIKSIAERRKKNAEWAVDAVRKSVSITEKEALEL
                     KVIDLIAENVDELLTQCDGRAVILPSGERILHTKDIAKRNININWRDKILQVITDPNI
                     AYILFSLGMLGLFFEFSNPGAFLPGIMGGICLILAFFAFQSLPINYAGILLMILGVIL
                     FLLEIKIVSHGALTIGGIISMLLGSLLLFQSPIPYMRVSLVIILCVVGATALFFIFAV
                     GSGLKAQMRKATTGAKGLVGQVAVARTDLNPEGTVFIHGELWRAESDTPVKTGEKTVV
                     KEVENLTLKVTKKQ"
     gene            892..1659
                     /locus_tag="VGB16_00955"
     CDS             892..1659
                     /locus_tag="VGB16_00955"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_006927791.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="slipin family protein"
                     /protein_id="HEX9916278.1"
                     /translation="MLSAGLLTVIVIAIFILANAVRILREYERGIIFRLGRLIGAKGP
                     GLILLIPIIDRMVKVSLRVITMDVPSQDVITRDNVSIKVNAVVYFRVMDAAKAIVQVE
                     DYLYATSMISQTTLRSVLGQVDLDDLLSSRDKINQQLQKIIDDQTEPWGVKVSVVEVK
                     NVDLPPEMQRAIAAQAEAERQRRAKIIHAEGEFQASQKLADAAKIIGEQPAAIQLRFL
                     QTLTEVAAEKNSTIVFPVPMDLFAPIIEALKRIKEKS"
     gene            1669..2316
                     /locus_tag="VGB16_00960"
     CDS             1669..2316
                     /locus_tag="VGB16_00960"
                     /inference="COORDINATES: protein motif:HMM:NF023410.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="EpsI family protein"
                     /protein_id="HEX9916279.1"
                     /translation="MNWKKADFWVVVGLVVLTGVLVNLLRYSEAKPQSRPHLSSVPLQ
                     LDDWQGGELRFGKATYEILKADESLLASFGSPKGQLWLFVAYFQSQKYGSQIHSPRHC
                     LPGSGWKIVKKEKMVTNFTTLRGKKLAMNRFVISDGTSQQLMLYWFVTRSGIITNEFG
                     LKMDLVFNSLQRKPTDAAFVRITIPFEQGKEQEAWGVATDFMNHFVPQIDQALGF"
BASE COUNT          696 a          485 c          626 g          651 t
ORIGIN      
        1 tgctgggaaa agtggagaac gatgcggctg cgtatatcaa aagcattgcc gagagaagaa
       61 agaaaaatgc cgaatgggca gtggatgcag tcagaaaaag cgtttccatt accgaaaaag
      121 aagcgctgga attgaaagta atagacctga tagctgaaaa tgttgacgaa ttattgactc
      181 aatgcgatgg cagggcagtt attcttcctt cgggcgaaag aatacttcac accaaagata
      241 ttgccaaaag aaatataaac ataaactgga gagataaaat tctgcaggtt attaccgacc
      301 cgaacatcgc ttacatactt ttttcgctcg gaatgttagg gcttttcttt gagttttcaa
      361 atcccggagc gtttttgccc ggaattatgg gaggaatatg tttgatcttg gcttttttcg
      421 ctttccagag tttgcccata aattatgccg gaattctttt gatgatactt ggagtaatac
      481 tttttctttt ggagataaaa atagtaagtc acggagcttt gaccatagga gggataatat
      541 ctatgctgtt gggttcgtta ttgctttttc agtcacccat accctatatg cgggtatcct
      601 tggtgataat cctgtgtgtg gtgggggcga ctgccttgtt tttcatcttc gcagttggtt
      661 caggtctgaa agcacagatg agaaaagcta ccactggagc gaagggactt gtcggccaag
      721 ttgcagttgc gcgcaccgac ttaaatcccg agggaacggt tttcattcac ggcgaacttt
      781 ggagggcaga aagcgatacc ccagtcaaaa ccggtgagaa aacagtagtt aaagaagtgg
      841 agaatttgac tttgaaagta accaaaaaac aataaacaat agggaggttt tatgttgagt
      901 gctggtctgt tgaccgtcat cgtcattgcc atattcatct tggctaatgc cgtaaggatc
      961 ttaagagaat acgaaagggg gattatcttt cgtctgggaa ggctaattgg cgctaagggt
     1021 ccgggcttaa ttctcttgat accgattatc gaccgtatgg tcaaggttag cttgcgggtc
     1081 ataactatgg atgtcccttc tcaagatgtt attactcgtg acaacgtttc catcaaagtc
     1141 aatgccgtgg tctatttcag ggtgatggat gctgccaaag ccatcgtgca ggtggaggat
     1201 tatctttatg ccacttccat gatttcccag acaaccttgc gcagcgttct tggtcaagtg
     1261 gatttagatg acctattgtc cagccgtgac aagataaacc aacaactaca aaagatcatt
     1321 gatgaccaga ccgaaccgtg gggagtgaag gtatctgtgg ttgaggttaa gaatgtggat
     1381 ttgcccccgg agatgcaaag ggcaattgct gcgcaggcag aggcggaaag acagagaaga
     1441 gcgaagatta ttcatgcaga aggggagttc caggcctcgc aaaaactggc cgatgcagct
     1501 aagattatcg gcgaacaacc agccgccatt caactgagat ttttgcagac tttgaccgag
     1561 gtggcagcgg agaagaattc aaccattgtc tttccagtgc cgatggacct atttgctccg
     1621 ataatagaag ctttgaagag gattaaagag aagagttaag aaataaaaat gaactggaaa
     1681 aaagctgact tctgggtagt ggtgggactg gtggtgctta ccggtgtttt ggtgaacctt
     1741 ctcaggtaca gcgaagccaa accacagagc cgtcctcacc tatcatctgt gcctttgcaa
     1801 ttggacgatt ggcagggggg ggagctgcgt tttggtaagg caacatacga gatactcaaa
     1861 gcggatgaaa gtctcctcgc atctttcggg agtcccaaag gacaactttg gctcttcgtt
     1921 gcctatttcc aaagtcaaaa atatgggtcg cagattcatt cccccagaca ctgcctgcca
     1981 ggaagcggtt ggaagattgt aaaaaaagag aagatggtaa ccaattttac gacactgcga
     2041 ggaaagaaat tggcaatgaa cagatttgtg atttcagatg gcacctccca acagttgatg
     2101 ctttattggt ttgtcacgcg cagtggaatc atcaccaacg aatttggcct gaaaatggat
     2161 ttggtgttca actccctgca aagaaagcca acggatgcag cttttgtgag aatcaccatt
     2221 ccctttgaac agggtaagga gcaagaggct tggggagtgg caactgattt catgaatcat
     2281 tttgttcctc agatcgacca agctttgggt ttttaggaac tatagcaaac acattaaatg
     2341 ttgttacata ttagagcaat atgcaatcta tcttaaaatc cccctccctt cgactttgct
     2401 caggatggtg aggacattcg gctgagcggt tcgaccgtcc tgagcggctc accgtcga
//
LOCUS       DASWLN010000028         2655 bp    DNA     linear   ENV 20-FEB-2024
DEFINITION  MAG TPA_asm: Candidatus Zixiibacteriota bacterium isolate
            SMAG_U2949 k141_536435, whole genome shotgun sequence.
ACCESSION   DASWLN010000028 DASWLN010000000
VERSION     DASWLN010000028.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37960594
            Sequence Read Archive: SRR10376091
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Candidatus Zixiibacteriota bacterium (soil metagenome)
  ORGANISM  Candidatus Zixiibacteriota bacterium
            Bacteria.
REFERENCE   1  (bases 1 to 2655)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 2655)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (26-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/12/2024 08:28:26
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 1,440
            CDSs (total)                      :: 1,416
            Genes (coding)                    :: 1,412
            CDSs (with protein)               :: 1,412
            Genes (RNA)                       :: 24
            rRNAs                             :: 1 (16S)
            partial rRNAs                     :: 1 (16S)
            tRNAs                             :: 21
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 4
            CDSs (without protein)            :: 4
            Pseudo Genes (ambiguous residues) :: 0 of 4
            Pseudo Genes (frameshifted)       :: 0 of 4
            Pseudo Genes (incomplete)         :: 3 of 4
            Pseudo Genes (internal stop)      :: 1 of 4
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2655
                     /organism="Candidatus Zixiibacteriota bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_536435"
                     /isolate="SMAG_U2949"
                     /isolation_source="soil"
                     /db_xref="taxon:2053527"
                     /environmental_sample
                     /country="USA: Oregon"
                     /lat_lon="42.99 N 123.63 W"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            <1..1396
                     /locus_tag="VGB16_00965"
     CDS             <1..1396
                     /locus_tag="VGB16_00965"
                     /EC_number="3.4.21.-"
                     /inference="COORDINATES: protein motif:HMM:NF017200.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004176 - ATP-dependent peptidase activity
                     [Evidence IEA];
                     GO_function: GO:0004252 - serine-type endopeptidase
                     activity [Evidence IEA];
                     GO_process: GO:0006508 - proteolysis [Evidence IEA]"
                     /codon_start=2
                     /transl_table=11
                     /product="S16 family serine protease"
                     /protein_id="HEX9916280.1"
                     /translation="FVGPPGTGKSSLAQSIAKALGRNFVRISVEGVRDEAEIRGHRMT
                     YTGALPGKILRAIRQAKCSDPVLLLEGVDKLNMEKVRGELTAALLEVLDPQFNRSFVD
                     HYLGIPYDLSGIMFIATAHILDNIPEPIQDLLEVIEFTGFIEEEKVEIAKRYLVPLII
                     RMHGLKTGDIEFTDHAIIRIIREYTVEAGIRTLQRGIEAICGYCARSKASGEDVSWKI
                     TEGNVEKYLGTPLYITEKIQKNPEVGVAIGLSWTEAGGDIMLIEALKMRGSGNVILTG
                     QLGDVMRESIQASHSYVRSKAELLGVNYDDFANYDIHIHFPGGAIPKDGPSAGVTICL
                     VLASVMSEKPIRNDIAMTGEVSLRGKVLPVGGLREKVSAAHRIGIRKVILPLENEKKL
                     ADLPEDVRSEMKFIFVERVEDVLKEALIDYEKPRRRSEDLLLDEIDRIKKSIQTRKPK
                     LKKIKHKNANRKHK"
     gene            1705..2511
                     /locus_tag="VGB16_00970"
     CDS             1705..2511
                     /locus_tag="VGB16_00970"
                     /inference="COORDINATES: protein motif:HMM:NF025690.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF4388 domain-containing protein"
                     /protein_id="HEX9916281.1"
                     /translation="MDLSGSIEKFDLHQIVHLLSVGKKSGTLGVQRGDEITMVYFQQG
                     GVIYAHSPTRPIGLGELLVQHKKITQEQLQEILNLQKELGNKKRIGELLVLESLITRS
                     DLESFVRKQVEEIIYRLLSWESGHFKFFEDRFPTQEEITINISTENLILESVRRQKKM
                     EKLKRRIPSLDAILSLAPSGDNRPKDLSLRADEWNVLTLVDGRRSIEEIISLSGLEKE
                     GALSKIAGLFLSGLVQKADNPKVEADSGALEDLVRQFSRLLEEYSKNEGI"
BASE COUNT          762 a          516 c          739 g          638 t
ORIGIN      
        1 tttcgtgggt cctccaggta ctggtaagtc ctctttggct cagtccattg ccaaggcttt
       61 gggaagaaac tttgttcgca taagtgtaga gggagtcaga gacgaggctg agatcagggg
      121 acataggatg acttacactg gtgcattgcc agggaaaatc ttaagagcaa taagacaagc
      181 taaatgcagt gacccggtgt tgttgctcga aggagtggat aagctcaaca tggaaaaggt
      241 cagaggcgag cttactgcgg cattgctgga agtgttagac ccgcaattca atcgttcttt
      301 cgtggaccat tatctgggga ttccctatga cttgagtggg atcatgttca ttgccactgc
      361 ccatattttg gacaatatac cggagccgat tcaggacctt ctggaagtca ttgaattcac
      421 tggattcata gaagaggaga aagtggaaat agccaagcgt tacctcgtcc cactgattat
      481 aaggatgcac ggtctgaaaa ccggagacat agaattcacc gaccacgcta ttataagaat
      541 aattcgagaa tatacagtcg aagcaggaat aagaacccta caacgtggca tagaagctat
      601 ttgcggttat tgcgcgcgca gtaaagcctc gggtgaagat gtttcctgga agataactga
      661 ggggaatgtg gagaagtatt taggtacacc actgtatatc actgagaaga ttcaaaagaa
      721 tccagaggtt ggagtggcaa tcggtctatc ctggaccgag gcgggtggcg atattatgtt
      781 gattgaagcc ctgaagatga ggggtagcgg taatgtaata ctgactggcc aattaggtga
      841 tgtgatgaga gaatcgattc aggcatcaca tagctatgtc cgttccaagg cggaactatt
      901 gggcgtgaat tatgatgatt tcgccaatta tgacatccac atccatttcc ctggtggcgc
      961 cattcccaag gatgggcctt cagccggagt caccatttgc ctggttctag cttcggtgat
     1021 gagcgagaag ccgataagaa acgatatcgc tatgaccggt gaagtctcgc tcagaggaaa
     1081 ggttcttccg gtgggaggtt tacgcgagaa ggtttcagcc gctcatcgca ttggtataag
     1141 aaaagtgata cttcccctgg agaatgagaa aaagttagct gatcttcccg aagacgtaag
     1201 aagtgagatg aaattcattt tcgttgaacg ggtggaggat gtgctgaagg aagctctcat
     1261 cgattacgaa aagccgagaa gaaggtcaga agacttgctt ctggatgaaa tagatagaat
     1321 aaagaaatcg attcaaacca gaaaaccaaa gctcaagaag attaaacaca agaatgcaaa
     1381 cagaaagcat aagtagctcc ttcctagttt tactcgcctc tgccagaaat ggcgaggaga
     1441 gcctcgccga agtcccgtag agcgggacga aggcgggctt aaaagaaggc tgcggtcgcc
     1501 tttggcgaac ctcgcttaag gcgggcgaag tctcgctcgc ctctcggcgc tcgcctctcg
     1561 gcgaagccga gggaagccga gggcttttta tatttgggag gccccgtgat ttatcatggg
     1621 gaagcttcac cttgactttt gagaaaccgg ttctatattt aagttaattt ttccatcgag
     1681 aaagatcttt acccgtgggg ggatatggat ctgtctggaa gcatagagaa gttcgattta
     1741 catcagatag ttcacctgct tagtgtgggt aagaaaagcg gaactttggg tgttcagcgg
     1801 ggagatgaga tcaccatggt ttattttcag caaggtgggg tcatctatgc ccacagcccc
     1861 accagaccga tagggctagg ggaacttctg gttcagcaca aaaaaattac gcaggagcaa
     1921 ctgcaggaga ttctcaatct ccaaaaggag ctggggaaca agaaaagaat tggagagctt
     1981 ctggtcttgg aatcgcttat cacccgcagc gatttagaga gtttcgtcag aaagcaggtg
     2041 gaggagataa tctatcgctt gttgtcctgg gagagcgggc atttcaaatt ctttgaagat
     2101 aggtttccca cgcaggagga gataaccatc aacatatcca ctgagaattt aattctggag
     2161 agcgtcagac ggcaaaagaa aatggaaaag ctcaaacgca ggattccttc cttagatgcg
     2221 atcttgtccc ttgcgccgtc tggggataat cggcctaagg atttgtcctt aagagcggat
     2281 gagtggaatg ttttgacctt ggtggatggc cgtcgtagca tcgaggagat aatctccctc
     2341 tccggcttgg agaaagaagg ggctttaagc aagattgccg ggttattctt gtccgggttg
     2401 gtgcagaaag cggataaccc aaaggttgag gctgatagtg gggctctgga ggatttggtg
     2461 agacaattct ctagactctt ggaagaatat agcaagaacg aaggaatcta aaataagtgg
     2521 aaaagaagaa aatcataatt gacctcaaaa acagggtgga gagctttctg gaaaatgttg
     2581 cccagaagga agagcagaag tttcgagcga aagccaaact ttctcagctg gaggatatag
     2641 tctatggtcg cagga
//
LOCUS       DASWLN010000029        20185 bp    DNA     linear   ENV 20-FEB-2024
DEFINITION  MAG TPA_asm: Candidatus Zixiibacteriota bacterium isolate
            SMAG_U2949 k141_919013, whole genome shotgun sequence.
ACCESSION   DASWLN010000029 DASWLN010000000
VERSION     DASWLN010000029.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37960594
            Sequence Read Archive: SRR10376091
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Candidatus Zixiibacteriota bacterium (soil metagenome)
  ORGANISM  Candidatus Zixiibacteriota bacterium
            Bacteria.
REFERENCE   1  (bases 1 to 20185)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 20185)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (26-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/12/2024 08:28:26
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 1,440
            CDSs (total)                      :: 1,416
            Genes (coding)                    :: 1,412
            CDSs (with protein)               :: 1,412
            Genes (RNA)                       :: 24
            rRNAs                             :: 1 (16S)
            partial rRNAs                     :: 1 (16S)
            tRNAs                             :: 21
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 4
            CDSs (without protein)            :: 4
            Pseudo Genes (ambiguous residues) :: 0 of 4
            Pseudo Genes (frameshifted)       :: 0 of 4
            Pseudo Genes (incomplete)         :: 3 of 4
            Pseudo Genes (internal stop)      :: 1 of 4
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..20185
                     /organism="Candidatus Zixiibacteriota bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_919013"
                     /isolate="SMAG_U2949"
                     /isolation_source="soil"
                     /db_xref="taxon:2053527"
                     /environmental_sample
                     /country="USA: Oregon"
                     /lat_lon="42.99 N 123.63 W"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            <1..508
                     /locus_tag="VGB16_00975"
     CDS             <1..508
                     /locus_tag="VGB16_00975"
                     /inference="COORDINATES: protein motif:HMM:NF012385.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0043565 - sequence-specific DNA binding
                     [Evidence IEA]"
                     /codon_start=2
                     /transl_table=11
                     /product="helix-turn-helix domain-containing protein"
                     /protein_id="HEX9916282.1"
                     /translation="DFRLICATNRDLELAVKDGKFREDLYYRLNVFSISIPPLRERRS
                     DIPLMANHFLKRLTTNMNKPVTGFSPEAMELLKNYDWPGNVRELRNAIERAVVVSKGP
                     FIHAEQLPIPSATGATPQKQTLEAVEKAHIQNILDQIGGNITHAAEVLGIDRATLYNK
                     IEKYGLRR"
     gene            511..1059
                     /locus_tag="VGB16_00980"
     CDS             511..1059
                     /locus_tag="VGB16_00980"
                     /inference="COORDINATES: protein motif:HMM:NF033823.0"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008233 - peptidase activity [Evidence
                     IEA];
                     GO_function: GO:0008237 - metallopeptidase activity
                     [Evidence IEA];
                     GO_function: GO:0008270 - zinc ion binding [Evidence IEA];
                     GO_process: GO:0006508 - proteolysis [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="archaemetzincin family Zn-dependent
                     metalloprotease"
                     /protein_id="HEX9916283.1"
                     /translation="MHLYILPLRFSDKNLLIDVSSALNDIFGLPVIILQNPFSLDEGR
                     DVLRNQVNSTWILSQLLKSAPTDPHKILGITELDLHIPVLTYVFGEAQLEGEAAVISS
                     YRLRDELYGLPKNQEKLKDRLRKEAIHELGHTFGLIHCRNILCVMHPSTYAEEIDFKS
                     MNFCKSCASILESKKKGSITKP"
     gene            complement(1113..2567)
                     /locus_tag="VGB16_00985"
     CDS             complement(1113..2567)
                     /locus_tag="VGB16_00985"
                     /inference="COORDINATES: protein motif:HMM:NF019310.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0022857 - transmembrane transporter
                     activity [Evidence IEA];
                     GO_process: GO:0055085 - transmembrane transport [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="MFS transporter"
                     /protein_id="HEX9916284.1"
                     /translation="MPRLTSEQIRKGLKISIWEGAFATVHLTLTSGIFLVGYALLLGV
                     NDFQLGLLAALPFLAQMVQPLAAFVVQNLARRKPFTLTGAVFFRGIWILLAFLPFTFL
                     TKNHKIVLFLSIIALSAVANNFATVAWLSWMADLVPQKIRGRYFGIRNTILQVVTICV
                     YFAGAKLIDHYKALKPDQLSQKLFDFSSFGNMSVLGFGIIFSLAVIAALVAGVLLYLQ
                     PEPSFPRFSNKAGLKDLFFPFKDKDYRKFIRFLVWWSLCVGIAAPFWGAHMLRNLHLD
                     YYLISLFSIVAGTISLVSQPFWGKMIDRYGSKPVLRFNLIFILFIPYLWFFVTPQNYF
                     LLWVDAVMGGIFWAGFNLAMFTIVLQISPQEGRPFFLASAYFVSGLFTFLASLIGGII
                     ANQLTTFKLQIYGQTLVNFHILFLLSALGRLWGLFVLGKVVEPESKPVGQMVSELGYY
                     FGNKFPGGAKVWEKIEEGLRLLGYQVGGRKENLR"
     gene            2981..4105
                     /locus_tag="VGB16_00990"
     CDS             2981..4105
                     /locus_tag="VGB16_00990"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEX9916285.1"
                     /translation="MKILVFVFLISACMVASAYGAGEIKKEFSVSEGGKLDIGLNSGG
                     SIDVRGWDKQLVSIGVDFVHCSAEDFIFDFSADSSTIKVRSDYKRHVNNSNIEVDIMV
                     PREFNLKLKTAGGSVSVYNVNGKIEGNTAGGSLDLKNLTGHIDLVTGGGSIIVKDSKL
                     DGEVRTGGGSVLVENVEGDFDAHSGGGNVVYKNVKTPSRVYSVEQVYIRNAGGDLNVD
                     DAPNGADISTGGGNIHIRSAKAFVRANTGGGNISIDSVDGSAEASTGAGEIEVTVISN
                     SQNGKGDITLTSGTGNITLTLPRYLSMDVDVELAYTKEHENRYRIISDFNLQQETTND
                     WDTSHGTPRKYIRGKTVLKDGQNKIVIRTVNGNVTLRQGK"
     gene            4561..4770
                     /locus_tag="VGB16_00995"
     CDS             4561..4770
                     /locus_tag="VGB16_00995"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002646585.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="cold shock domain-containing protein"
                     /protein_id="HEX9916286.1"
                     /translation="MSKGTVKRIMRERGFGFILAEDGREIFFHRSELQDVNIEDLNEG
                     DHLEFTVTKGDKGPQATGVKKISES"
     gene            4826..6157
                     /locus_tag="VGB16_01000"
     CDS             4826..6157
                     /locus_tag="VGB16_01000"
                     /inference="COORDINATES: protein motif:HMM:NF015983.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003824 - catalytic activity [Evidence
                     IEA];
                     GO_function: GO:0051539 - 4 iron, 4 sulfur cluster binding
                     [Evidence IEA];
                     GO_function: GO:1904047 - S-adenosyl-L-methionine binding
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="radical SAM protein"
                     /protein_id="HEX9916287.1"
                     /translation="MRILLTTPSLDKVGSRFLRMPQLSLGVISALTPKEIEVDVVEEG
                     IEEINFDKCYDLVGISCMTAAAPRAYQLSSVFRKKGAKVVLGGVHPTVLPQEAIAHCD
                     SVVIGEAEGCWKELLDDFRGNRLKKFYQCFDFDLAKFPIPRLNHNHTPFHVTPILSTR
                     GCPYACEFCSVTDLYGKKLRHRKVEDIVKEIATRGSTKFFFLDDNITGDVKFAKELFA
                     ALSDLRIRWVGQASISLVKDKVLLGLAKKSGCAALFIGIESVSSDTLKKLRKTPNDAK
                     EYGQAIKIIQDCGILFHASLVFGFDDDDKSIFEKTLDFLEKTKIASATFNILTPYPGT
                     TLFERFKKEGRLLSTDWREYNHRTVVFKPKNMTPEELALGFIWLGRNFYTKASIFTRF
                     FHNFTHPLLYFSLNWGLRNIFENDKVEDCLPHSPIAISGSEPRASEYLQRG"
     gene            6813..8228
                     /locus_tag="VGB16_01005"
     CDS             6813..8228
                     /locus_tag="VGB16_01005"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEX9916288.1"
                     /translation="MKKKTKKTITFAVLISFFIGAFVGEITHGLVSLSWGFAKNLIFP
                     TKLELAFTKFIIYRTESDQFPTCDMDIVARSNSNENLTVELKELRFFGRDIIYRFGAG
                     QQVIKTQGVDVFSKHITFRDKVLDDALKVPQDSNTAILELKYQYVGAKSTATLKLTDK
                     DVIKCTYWQSPVFNSDIEAAGFGAIPFSIAGRVYWHTDKPHRKKGSWDPNIKVFINQY
                     GLYDFDMTEFGGRIISKDFSLSYYPPAFKRKYGGVYLGFLASDSAEFERATFPHQLFG
                     NLRDYDEYNLTGSTFDQDFSKVKEFIYYDFDPARRQKLFEICYENSKYYILLVYEKPE
                     GIRPLAQNLAAKGYRVGSVSANFFGGLAEVMTLITPEEKLPSLAERFNAYVDSLNSVI
                     NNDGVFVFFPVALDAKTINRITSDIRQLTGKERSVAFDFDCRPFKSRLPNAPFSTPAL
                     TFLNFSQTTMNAKLDSLGVKW"
     gene            complement(8711..10192)
                     /locus_tag="VGB16_01010"
     CDS             complement(8711..10192)
                     /locus_tag="VGB16_01010"
                     /inference="COORDINATES: protein motif:HMM:NF017044.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0046872 - metal ion binding [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="pitrilysin family protein"
                     /protein_id="HEX9916289.1"
                     /translation="MKKIFIVFVSFLLLLGFSCAKQEGLKLIEVKKPTIPVVYFACMI
                     KSGSAYDPAGKEGLSYFTAELLRRGTKSFTREQIDDTLDLISGDINIRTSREVIVITG
                     RTLKSNLEKFYPIFSEIILAPSFPPEEVEKLKTDQKSAIDGLIQDDAELVKEGFMEYI
                     FQGHPYGHSPLGRYSTIEKFNQEDVKDFYAKHFLKNNIILGLGGDIDNNLVAKVKKDF
                     SVLKVGEVPQLNGTITPVQGRKVFLIEKEGRDQVQLRFGFPYELKRQDKEYFPILVAD
                     TYFGKHREMFGVLFRIVRAARGLSYGAYSYFEEFEQYGWSNLALPNNPLLHQYFSCWT
                     YPKKVNAKFTIKLVLKLLTDLVNDGIPENGLKQAKEYEENHFPFEIETPDRLLGMKMD
                     EEFYGTKTFLKDFGTNVEKVTKGELDAALKKFISTDNIAIIGVVSNGEQFKAELLSKR
                     TVIEYPTGAAGASLQKEDSEIMGFDLKLKSEDFKIVRAGEMFK"
     gene            complement(10212..11549)
                     /locus_tag="VGB16_01015"
     CDS             complement(10212..11549)
                     /locus_tag="VGB16_01015"
                     /inference="COORDINATES: protein motif:HMM:NF017044.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0046872 - metal ion binding [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="pitrilysin family protein"
                     /protein_id="HEX9916290.1"
                     /translation="MKRFPRIALLFLTLFFLSELCLAQTDQMKLGVKKTVLPNGLIVL
                     TCEDKTVPTVSYMTFVNAGSRDEVKPGTTGLAHVFEHMMFRGTEKFPSYYSATAPFGA
                     ETNASTGEDYTMYYVNAKEQFLDSIVQIEADRIINLPFDNETFRTELGPIKEERRRFE
                     VDDPNGFLEGEFTQLAYTVHTYHHPVIGFEEDLEKNIQLQDGIDFKNRFYAPNHCAIV
                     IAGNFETPVLLRLIEKYYGGWKKATYPDNTISPEPTQKKERIKNYVWKDKETSPKMKI
                     GFHSPKLDLNNDDYCALRLLQKVLFLPSGRITERLVKDLQLVEWVWGGVGENKDPGMF
                     TISVSLKKGKSLEEVKKVIYEELEKTKNQLVQKEELEKAVNSEKAGVLYQLDRPSSIA
                     YFLGHYQTLVGDYEYLWKLPERFEEITPEMIQKAAQDIFIPTNRTVVTLVPKS"
     gene            complement(11777..13609)
                     /locus_tag="VGB16_01020"
     CDS             complement(11777..13609)
                     /locus_tag="VGB16_01020"
                     /inference="COORDINATES: protein motif:HMM:NF013460.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0016020 - membrane [Evidence IEA];
                     GO_function: GO:0009055 - electron transfer activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="cytochrome b/b6 domain-containing protein"
                     /protein_id="HEX9916291.1"
                     /translation="MGRIFRTLALLFVFSIYSFSGVSAQTKEECLVCHDQPGLNTLDN
                     SVHAKLSCVACHKGVRPEVHETKPGKIDCGICHPTPLKSYKEGVHGKALEQNLKNAPD
                     CQDCHGAHDIRKIADKESKVSRINLPGVCGRCHPQARSDFAGSVHGELLIKEKMEESL
                     TCSDCHHSHATESPLDAKSLASKANIPQLCSKCHIDSYEEYKGDVHGRALERGILRSA
                     TCTDCHGAHNILAAGDTNSTIYPANISKLTCSGCHLQEKVVKQFGVTEKKLTGYHDPY
                     HGVEVMAGDKRMPDCSDCHSAHNVRPSSDARSTVSRENLPKTCAKCHTGAGVNFARGK
                     FHFSDEEKGASFIRSLYTFLIILVIGLMFLHNLLDLIRHAIDKYRQSAKEEILRFTLS
                     ERLQHILLFITFTLLAYTGFVLRFPDFFLFSWMARSAANMAMRAMVHRIAASIFIALC
                     LYNLYYILFTARGKEQLKAIFPVPNDMLQAIQNVLHKLGLLRKKPKFDRYNYGEKAEY
                     WALVWGGFVMIFTGLVLWFPDFSLRFVPKWLLDVFKSIHFYEALLASLAIIVWHFYFV
                     FFSPEIYPINWGMTTGRISKEELDEKHSAEYERLKEKGEIDEWD"
     gene            complement(13732..14523)
                     /locus_tag="VGB16_01025"
     CDS             complement(13732..14523)
                     /locus_tag="VGB16_01025"
                     /inference="COORDINATES: protein motif:HMM:NF014017.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF72 domain-containing protein"
                     /protein_id="HEX9916292.1"
                     /translation="MIKIGTSGFSFDSWIGPVYPTYLKKNQLLLYYERILGFNVLEIN
                     STYYALPSRKSMESLVKRTSPDFRFSIKANSEMTHKIKDKKNNKKSFENFKFSIQPFV
                     DSGKLLCILAQFPYSFYPAKDNFDYLKQFKEQVLGIPIVIEFRNRYWHKSEVLDFLKD
                     KDLGYCIVDEPNLKGLMPFNPAITSDIAYFRFHGRNKDWFEAEKSSRYDYLYSQEELK
                     KFVDPIKDLDSKASQTLVFFNNCHIGKAVRNALMLKEIIQNDSKR"
     gene            complement(14540..15778)
                     /gene="dinB"
                     /locus_tag="VGB16_01030"
     CDS             complement(14540..15778)
                     /gene="dinB"
                     /locus_tag="VGB16_01030"
                     /EC_number="2.7.7.7"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011938059.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003684 - damaged DNA binding [Evidence
                     IEA];
                     GO_function: GO:0003887 - DNA-directed DNA polymerase
                     activity [Evidence IEA];
                     GO_process: GO:0006281 - DNA repair [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="DNA polymerase IV"
                     /protein_id="HEX9916293.1"
                     /translation="MSDRTILHVDMNAFFASVEQRSNPYLRGKPILVCGNPKGRTVVA
                     TASYEARKFGIKTGMTIYQAKKLCPDAILVECDPDKYVDTATKLFNIYIDFTPQVEVF
                     SIDEAFLDITGTERLFGTPVEIAQKIKQRIKNELGLACSVGIGPNKLLAKFASESKKP
                     DGLTVLTKDKVLEILENVPISELCGIGTKMTQHLNKFGIYTCGQLGRYPFEILQRRFG
                     IIGLYLHHMGLGEDESPVRYYYEKEPVKSMGHSLTLQEDAHDKEIVKRHLLQLSEKVG
                     RRLRRENYQGRTVTLILRYADFETVAKQHTLKKFIDDGKLIYEATLGILDTLFIQHRG
                     VRLTGVSVSNLIQGAKQLDILPEERRREGLLYALDQINDKFGEFTITYAKLLKPAIEG
                     NVISPAWRPHKHIPGFKVAT"
     gene            complement(15780..16034)
                     /locus_tag="VGB16_01035"
     CDS             complement(15780..16034)
                     /locus_tag="VGB16_01035"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEX9916294.1"
                     /translation="MVTQIDEMIKVVAIFGDGKKLKPIYFLWGNRKYKISQITYNWIS
                     QDGKAKVCHFSVIDTNDNLFEICYNTESMVWKLANIETEG"
     gene            complement(16390..17493)
                     /locus_tag="VGB16_01040"
     CDS             complement(16390..17493)
                     /locus_tag="VGB16_01040"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEX9916295.1"
                     /translation="MRRALMLLAIGLLFVVANGQHSQAADVSGTAPTVSSSGHDTEIN
                     AILADAFNIAIDDASDNLAKFKRMENLAKGFGNANVYSSQVATLQGYQNYLHCAVTVG
                     AMVGVQLPSLDPDYYNDLEDVIDKDGDIYAGVGAGISFINLGFNASSLHPGLYMNVKF
                     GTLSLKPQEDVEIKNTVFGVGANYCLVKGVGEGQKLFKWRGLSLGTGLLYHSSKVDFK
                     IEQDEIAEDLVGLDPYYPGTNAQLLLDPSFNLGLDVKTTTIPVDISTSVQLLWLFNLN
                     LGAGVDFNFGSSDIVLSSKGGVRVEGLPDTVSQTAGKVRVNGSTLGISPSSACGRIMT
                     GIGFNFSMVKIDVPVIWYPSKGASVGVTAGVVW"
     gene            17716..18957
                     /locus_tag="VGB16_01045"
     CDS             17716..18957
                     /locus_tag="VGB16_01045"
                     /inference="COORDINATES: protein motif:HMM:NF024241.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0022857 - transmembrane transporter
                     activity [Evidence IEA];
                     GO_process: GO:0055085 - transmembrane transport [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="MFS transporter"
                     /protein_id="HEX9916296.1"
                     /translation="MSLPKFSRFLGLIRSYDSRMWILFSGWMVSSMGFAMVIPYLSIY
                     FHNQMKIPMAQVGLLFGIGAIVRALSGLYGGNLSDKLGRVKIMGWAQFLRGVFFLLMG
                     WLAWKGGSFLLWSLVLILNWLFGGFYQPVANAMAADMAPKEKRVEAYSILRIAGNIGW
                     AFGPMIGGFLASTSYPILFVFASLSTFISSFLILSFLPETIKINHSEEKFTLKDMGKL
                     KSDHLFLLYCSICLLLFVVIGQLVSTISVFSVDYVGISESNLGFLYAINGFVVIFFQL
                     AVTKLTQRRNLIQVLFWGSLGYSLGYFLVGFSSSFAWLFVCMLLISFSEITVLPASMA
                     IVANLSKEKHHGLYMGGYGLFYSLGWSFGPLVGGVLIDLLNNKPVLLWGIIASLGILS
                     ALGFSWIGKKLPREAILPPAK"
     gene            complement(18982..19104)
                     /locus_tag="VGB16_01050"
     CDS             complement(18982..19104)
                     /locus_tag="VGB16_01050"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEX9916297.1"
                     /translation="MEKQSASKGISPLIWVIIGILALGAGTLIFWAVIMMFFRR"
     gene            19377..>20185
                     /locus_tag="VGB16_01055"
     CDS             19377..>20185
                     /locus_tag="VGB16_01055"
                     /EC_number="3.1.1.103"
                     /inference="COORDINATES: protein motif:HMM:NF012371.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016787 - hydrolase activity [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="serine hydrolase domain-containing protein"
                     /protein_id="HEX9916298.1"
                     /translation="MRKNLTSFISLIVILGFACMAYAGNEATVRFPQTNAGKLAESYF
                     SAFNSHNENDMRTYLEQNFAPNTLKERPIEERMAVYRQLKSDLATLEPVKILKESTDA
                     IKLVARSAKEAWLELTFEFEKNAPYKLLGIRIMRLEEAPDLSAPTSLMTETEMLKELN
                     TFMDGISVKDEFSGVVLVAKGQKPIFRKAYGLASKEYNVPNRVDTRFNLGSISKFLTR
                     IAIEQLQGKGKLSLDDPIGKYLPDYPNKEAAENVTIRHLLEMTSGIGDFFG"
BASE COUNT         5738 a         4444 c         4261 g         5742 t
ORIGIN      
        1 cgatttccgc ttgatatgtg ctaccaatcg cgacttggag ttagctgtta aagatgggaa
       61 atttagagaa gatttgtatt atcggctcaa cgttttctcc atctccattc caccgctaag
      121 ggaaagacgc tcagatatac ctcttatggc caatcacttt cttaagagat taaccactaa
      181 tatgaataag cccgtaactg gattttctcc tgaggctatg gagctattaa agaactatga
      241 ttggccgggc aatgtgaggg aactaagaaa tgcaatagaa agagctgtag tagtatccaa
      301 agggcctttc atccatgcgg aacaacttcc cattccaagt gcaaccggag ctacacccca
      361 aaagcagact ctggaagcgg tcgaaaaagc ccatatccag aatatcctgg atcagatagg
      421 aggaaacata actcatgcgg ctgaagttct gggaatcgac cgggctaccc tctataataa
      481 gatagaaaag tatggcttac gaagataaaa gtgcacctat atatacttcc attacgtttt
      541 tcagataaga atctcctgat tgatgtatct tccgccctca atgacatttt cggacttccg
      601 gtcatcattt tacaaaaccc tttttcactg gacgaaggcc gagacgttct tcgcaatcag
      661 gtcaattcca cttggatttt atctcagctc ttaaaatctg cccccacaga cccgcataag
      721 attttaggga taactgaact cgacctccat atccctgttt taacttacgt ttttggcgaa
      781 gctcaattgg agggtgaagc cgcagttatc tccagctacc gactcaggga cgagttatat
      841 ggactgccta aaaatcagga aaagttgaag gaccgtctgc gaaaggaggc aatccacgaa
      901 ttgggacaca cctttgggct gattcactgc cgcaacatcc tgtgtgtgat gcatccatca
      961 acttacgctg aggagatcga ctttaaatcc atgaatttct gcaagagttg cgcttctatt
     1021 ttggaaagca agaaaaaggg gtcaatcact aaaccttaga agttatcgaa gaatattgcc
     1081 gaatcccgat tcaaatgcat catgcttgaa tcttatctca aattttcctt tctccctccc
     1141 acctgatacc ccaagagcct caaaccctct tctatctttt cccagacttt cgccccacca
     1201 gggaatttat tcccaaaata gtaacccaat tcgctcacca tctggccaac cggcttgctc
     1261 tccggctcta ccacttttcc caagacaaac agaccccata atctccccag agcagataaa
     1321 agaaaaagaa tatggaaatt caccagggtc tgtccataaa tctggagttt aaaggtggtg
     1381 agttggttag cgataattcc gccgataagt gatgccaaaa aagtgaataa cccacttacg
     1441 aaataagctg aagccaaaaa gaaaggtctc ccctcttgcg gagaaatctg gagaactatg
     1501 gtgaacatag ccagattaaa tcctgcccag aaaatcccgc ccatcaccgc gtccacccat
     1561 agtaagaaat agttctgcgg agtaacaaaa aaccataagt aaggaataaa tagtatgaaa
     1621 atgaggttga atcttagaac cggcttggag ccatagcggt caatcatctt accccagaat
     1681 ggctgggaaa ccaaactgat tgttccggcg acaatagaga aaagggaaat caaatagtaa
     1741 tctaagtgta aattcctgag catatgcgct ccccagaagg gagcggcaat tcctacgcac
     1801 aaagaccacc agactaaaaa gcggatgaac ttacggtaat ctttatcctt aaagggaaag
     1861 aataaatctt ttaagccagc tttgtttgaa aaacgaggga aagatggttc gggttgaaga
     1921 taaagcagaa ctcctgcaac aagcgctgct atcaccgcca gagagaatat tatgccaaat
     1981 ccgagaacgg acatatttcc aaaactcgaa aaatcaaata gtttctggct caactggtca
     2041 ggtttgagag ctttgtaatg gtcaatcagt ttcgctccag caaaatagac gcatattgtg
     2101 acaacttgca agatggtgtt tctgattccg aaatagcgac cgcgaatctt ttgtggaact
     2161 aaatccgcca tccaggaaag ccaggcaact gtagcaaaat tattcgccac cgcgcttaat
     2221 gcaatgatgg aaagaaacaa aacgatttta tgatttttgg ttaggaaggt gaagggaagg
     2281 aaagccagca aaatccatat ccccctaaag aagaccgcac cagtcagagt gaaaggcttt
     2341 cttcttgcca gattctgaac cacaaatgct gccaggggct gaaccatctg agccagaaaa
     2401 ggtagagctg ccagaagtcc caattgaaaa tcgttcactc ctaaaagcaa ggcatatccc
     2461 accaagaaga tgccactggt aagggtcaaa tgcacagtgg cgaaagcccc ctcccaaatt
     2521 gatattttta atccctttct gatttgttca ctggttaatc tgggcatgtc acttttccct
     2581 caataactgt aatatttaca aaaaagaaac ggaaaaatca agaaagaaga atgacaatga
     2641 caaaaagaag tgaggtaagc tcccatttct gcaaggcctt gttttccccc ttgcataact
     2701 tcaaataaga ttcatattat tgaagatgga tttgggttta atggatcctg tcaggagtag
     2761 ctcctgacat ttttttgtta gtgcgcaggg cggctaaact actctccgca aatacaaaga
     2821 atctgcaacc gaatcaccaa gacagcgtaa gatagaggta agattacata aatagttaaa
     2881 cgcagggcat tacaagttgc atgatcggct ctcggcttct gtcagtcata attcgtcaaa
     2941 cagaaacatg taacccatta accaaaggat ggaggacgtc atgaagatat tggtttttgt
     3001 tttcctcatt agcgcttgca tggttgccag cgcttatgga gctggtgaga tcaagaagga
     3061 gttctcagta tctgaaggcg gaaagctgga tattggcctt aactctggtg gctcgattga
     3121 tgtccgcggt tgggataaac aactggtgtc aataggggta gatttcgtac attgcagtgc
     3181 agaagatttc atttttgatt tttcagccga ttcctctacc atcaaggtgc gttcagacta
     3241 taagcggcac gtaaacaata gcaacattga ggtagacatc atggttcccc gagagttcaa
     3301 cttgaagcta aagacagccg gcggtagcgt cagcgtctat aacgtcaacg gcaagataga
     3361 agggaatact gccggcggca gcctagatct taaaaaccta accgggcata ttgacttggt
     3421 gacagggggt ggttctataa tagtaaaaga ttcgaagctg gatggagagg ttagaaccgg
     3481 tggtgggagt gttttggttg agaacgttga aggtgatttt gatgcccatt ccggcggtgg
     3541 aaacgtcgtt tacaagaacg tcaagacgcc atcccgggtc tattctgtcg agcaggtcta
     3601 tataagaaac gccggtggcg accttaatgt cgatgatgcc cccaatggcg ctgatatcag
     3661 caccggcgga ggaaacatac atatacgttc agccaaagct tttgttcgcg caaacaccgg
     3721 aggggggaac atcagcattg attctgttga cggttcagcg gaagcatcca ccggagccgg
     3781 cgagatagag gttactgtaa taagtaattc ccagaatggc aagggcgaca taacacttac
     3841 ctccggaacc ggaaatatca cgctaaccct gccgcgctat ttatccatgg atgttgatgt
     3901 cgagctggct tatacaaagg agcatgagaa caggtacagg atcataagcg attttaacct
     3961 tcaacaggag accaccaacg attgggacac aagtcacggc actccccgca aatacatccg
     4021 ggggaagacg gtcttaaaag atggccagaa taagatcgtg attcgcacgg ttaatggtaa
     4081 tgtcacttta aggcaaggta aatgatcttg tggggattga ggaggaagag ctgcagaatt
     4141 cataaagaca gcatctctcg gcagtttctt tccaatccgc ctgaaaccca aagcagatga
     4201 agctccctgc gccaagagcg cagggtatct taagcggaat tcatccgaag ccaaccatct
     4261 ttcgctctac gagctacgga atggttctcc tcgtcttcat ccctgcggca agaccgcagg
     4321 gtattcggcg aaggagaata aaaggcccaa aaaggcgatt atcccctact gcagttatta
     4381 gtaaccctaa atataatttg acatttaaaa aataaaaggt tatataagaa tgacaaagat
     4441 tttaagttaa ccattaaaat ttaaagaaag gggtctcagg gctaaattat ttattaagga
     4501 agttaaaccg gggcaatttc tttaaaaaaa ttgggtattt ttttcaaaag gaaggaaaat
     4561 atgtctaaag ggacagtaaa gcggataatg agagaaagag gatttggatt catcttggct
     4621 gaagatggaa gagagatatt ttttcatcgc tctgagctcc aagacgtgaa catcgaggac
     4681 ttgaatgaag gagaccatct ggaatttacc gttactaaag gcgacaaagg ccctcaggct
     4741 accggcgtca aaaaaatcag cgaatcgtag agattaattc cgccctgcat ttggctgagt
     4801 taggactcca gaataagaag atgagatgag gattcttcta acaactccct ctttggacaa
     4861 agtcggaagc agatttttga gaatgcctca gctcagctta ggtgttatat ctgccttgac
     4921 tccaaaagag atagaagtgg acgtggttga ggaagggatc gaggagatca atttcgacaa
     4981 atgctatgac ctggtgggta tctcctgtat gactgcagcc gctccccggg cttaccagct
     5041 ctcttcggtt ttccgaaaaa aaggcgctaa ggtggttttg gggggagttc atcctacagt
     5101 tctaccccag gaagcgatcg ctcattgcga tagtgtggtt attggcgagg cagaaggatg
     5161 ttggaaagag ctcttagacg atttcagagg gaatcggcta aagaagtttt accaatgttt
     5221 tgattttgac ttagccaaat ttcccatacc cagactgaac cacaatcata cccccttcca
     5281 cgtgaccccc atcctctcca ccagaggttg cccctacgct tgtgagttct gttcggttac
     5341 tgatctatat ggaaaaaagt taagacaccg caaagttgaa gatatcgtca aggagatagc
     5401 cacaagaggc agcacaaaat ttttcttttt agatgacaat atcacagggg atgtcaaatt
     5461 cgccaaggaa ctcttcgccg ctctttcgga tttgagaatt cgctgggtgg gtcaggcatc
     5521 catatcttta gtcaaagaca aagttttgct tgggctggcg aaaaaatctg gttgtgcggc
     5581 tctgttcatc ggaatagagt cggtttcctc tgatactctt aagaagctcc gtaaaacccc
     5641 gaatgatgct aaagagtatg gccaagctat caagatcatt caggattgcg gaatcttgtt
     5701 ccacgcctca ctggtgttcg gctttgacga tgatgataag agcatattcg agaagaccct
     5761 ggactttctg gaaaaaacga aaatcgccag cgccactttt aatatcttga ccccctatcc
     5821 gggcacgact ctcttcgaga gattcaagaa agaaggaagg ctcttaagta cggactggcg
     5881 ggagtataac cacagaaccg tggttttcaa acccaaaaat atgaccccgg aagaattggc
     5941 tttaggtttt atctggttag gaagaaattt ttatacaaaa gcttcgatat ttaccagatt
     6001 ttttcacaac ttcactcatc cgctgctcta tttttctctt aactgggggc ttcggaatat
     6061 ttttgaaaat gacaaagtgg aagattgcct cccgcatagt ccaatcgcta tatccgggtc
     6121 tgagccacga gcttccgaat atcttcaaag aggatagaat attgtattcg ctaatcagag
     6181 aagttccaaa atcgtccact acacccagaa aggcacttta aaagttttga gtttagagac
     6241 cccacggcaa agtagttttg agattaagtt ttgagaaatc tgtagttgct ccatttatgg
     6301 agcgaagtta agagaccgcc cgataaatcg ggcaactaca aaagtataac ctctccctaa
     6361 ccctctcctt atgtaaggag aggacaaagg tgaggataaa aaagcagttt aaaaatttaa
     6421 taggttgtgc caggagtaac tcctgacact gtttatttgt caggtgctcg gatgagttca
     6481 tctgggcgca gggcacataa actataatac taattaagga gggacaacat gaaaccagtt
     6541 acgactctgg caaccattat cttcgcgcta atttcaatcg gacatctatt acggctaatt
     6601 tttcaagtac aggtagtggc ggcgggcttg gttataccga tgtgggtcag catcttcggg
     6661 tgccttgtcc cggcaacaat cgccgtattg atgtggcgtg agaatcgacg tgcataagga
     6721 gaagtagtag gtcaggtgct cggctgagcc catcttggcg cagggtgccc gagttgcgtg
     6781 gccatgagac cattaaccct attcttaatt gtatgaagaa gaaaacaaag aaaactataa
     6841 cctttgcagt actcatatcc ttttttattg gagcttttgt aggtgaaata acacatggtt
     6901 tagtttcttt atcttggggt tttgcgaaga acctaatatt ccctacaaag cttgagcttg
     6961 cgtttacgaa attcatcata tatcgaacgg aatccgacca gtttcctact tgtgatatgg
     7021 atatagttgc aaggagtaat tcaaatgaga atttgacagt ggaactgaag gagttaaggt
     7081 ttttcggtcg agatataata tatcgattcg gtgcgggtca acaagttatt aagacacaag
     7141 gagtagatgt attctcaaag cacataacat tccgggataa ggtattggat gacgctttga
     7201 aagtacccca agattcaaac acagccatat tggagctaaa atatcagtat gtaggtgcaa
     7261 aatcaacagc cactctaaaa cttactgata aagatgtaat aaaatgtaca tactggcaga
     7321 gccccgtgtt taactccgat atagaagcag ctggttttgg agcaataccg ttcagtatcg
     7381 ctggcagggt ctattggcat actgacaagc cacaccgcaa aaaaggtagc tgggatccta
     7441 atatcaaagt atttataaac caatatggtt tatacgattt cgacatgact gaattcggcg
     7501 ggcggattat cagtaaggat ttctctttga gttactaccc tcccgcattc aagcggaagt
     7561 atggaggagt ttatcttggg tttttggcat cagattccgc agagtttgag cgtgccacgt
     7621 ttcctcatca attatttggc aatcttaggg actatgatga atacaatttg actggttcaa
     7681 ctttcgatca ggatttttcc aaggtaaaag agttcattta ttacgatttc gacccagcta
     7741 ggaggcagaa gttatttgaa atttgttacg agaattcgaa gtattacatt ttgctagtat
     7801 atgaaaaacc tgagggaata cgaccattag ctcaaaacct agccgcaaaa ggatatagag
     7861 tgggatctgt gtctgcgaac ttctttggtg gacttgcaga ggtcatgaca cttataactc
     7921 ctgaggaaaa attacctagc ttagcagaaa gatttaacgc ttatgtagac agtcttaatt
     7981 ctgtaataaa caacgatggc gtttttgtgt tttttcctgt ggcattggat gctaagacaa
     8041 taaatagaat tactagcgat atacgtcaac ttacaggtaa agagcggtca gtagcatttg
     8101 atttcgattg ccgacctttt aagtccaggt taccaaacgc accgttttcg actccggctc
     8161 ttacatttct taatttctct cagacgacta tgaatgcgaa acttgacagt ctgggtgtga
     8221 aatggtaggt caggagcccc gtacttttga cactattttt tgatgcttcg ggaatagaaa
     8281 ttcccgaacc acaaattctg tgtcttaaga cttaaatgca tcggcatttc tcctccgcca
     8341 aaggcgagag atcttgagac taatgctgat gcatcaaaaa ataaatatgg atccccgatt
     8401 aaaccctcgg ggatgacaaa atatagcatc aagtgcggaa tgacatatca ctcctgatat
     8461 ccagatacaa atagctaagt agcttagggg ttccctgctc ctgtgttaag tgcgcacggc
     8521 acctgaccta ccaactaagt gcaattggga accacgaacc cctcttaatc tccccttatc
     8581 aggggagaaa aatatcatgg tccccctgac aagggggaca acagggggtt tgtcagcact
     8641 ccatatcata atgacaaaaa taaagggcag gagaaattcc tgccctttat ttgccttaga
     8701 gtcgtcttat ttatttaaac atctccccag ctcttactat tttgaaatcc tcggatttta
     8761 gtttcaggtc aaaacccatt atttcactat cctccttttg cagggaagcg ccggctgctc
     8821 cggtaggata ttcaatcact gttcgttttg acaaaagctc cgctttaaac tgctctccat
     8881 ttgacaccac tccaattatg gcaatattat ccgtagaaat gaatttcttt aatgcggcat
     8941 caagttcccc tttggtaact ttctctacgt ttgttccaaa atccttcaaa aaggtcttgg
     9001 tgccatagaa ctcctcatcc attttcattc ccagaagcct atccggagtt tcaatctcaa
     9061 aggggaaatg gttttcttcg tattcttttg cctgttttag accattttcc ggaatcccgt
     9121 catttaccaa atcagtcaaa agtttcaaca ccagctttat ggtgaatttg gcattgactt
     9181 tcttgggata agtccagcaa ctgaaatact gatgcaaaag aggattgttg ggcaaagcta
     9241 aattagacca gccgtattgt tcgaattcct caaaataact gtaagcgccg taggacaatc
     9301 cccgtgcagc cctgacgatt ctaaataaca ctccgaacat ctcccgatgt ttgccaaaat
     9361 aagtatcagc taccagaatg ggaaaatact ctttatcctg tcttttcagc tcataaggaa
     9421 agccaaagcg gagctgaacc tggtctcttc cctctttttc gattaagaac acttttcttc
     9481 cctggacagg ggttatagtt ccattcagtt gcggaacttc cccgactttc aaaacagaaa
     9541 aatccttctt aacctttgcc accaggttat tatcaatgtc accacccaat cccagaatga
     9601 tattattctt caggaagtgc ttagcataaa aatcctttac atcctcttga ttaaacttct
     9661 caatagtgga gtatcttcct aaaggagaat gtccatatgg atgaccctgg aaaatatatt
     9721 ccataaaccc ttcctttacc agctcggcat catcctgtat caatccgtca atggcgctct
     9781 tctggtctgt ctttagtttc tccacttctt ctgggggaaa gctcggcgcc aggattatct
     9841 ccgagaagat tggatagaac ttttctaaat tactttttag agtcctgccg gttataacta
     9901 ttacctcgcg actggtccgg atattgatat ctccgcttat caaatccagg gtgtcgtcaa
     9961 tttgctcgcg ggtgaaggac tttgttcctc tccttaacag ctcagcggta aaataagaaa
    10021 gcccttcctt gccagccggg tcataagccg agccggattt tatcatacag gcaaaataaa
    10081 ccaccggaat agttggcttc ttcacctcga ttagttttag tccttcttgc ttagcgcagg
    10141 agaagccaag caagagcagg aaggaaacaa aaacgatgaa gatctttttc atttttcctc
    10201 ctattactct tttaagattt aggcaccaaa gttaccacgg tccgatttgt cggaataaaa
    10261 atatcctggg ctgccttctg tatcatctcc ggcgtaatct cttcaaacct ctccggcagt
    10321 ttccacaaat attcataatc cccaaccaag gtctggtaat gccctaaaaa gtaggcaatg
    10381 gaagaaggtc tgtcgagctg atacaaaacc ccggcttttt cggaattgac tgctttttcc
    10441 aactcctcct tttgcactaa ctggtttttg gttttctcca gctcttcata aatgactttt
    10501 ttcacttctt ccagcgactt accttttttc aaactaactg aaatggtgaa cattcccggg
    10561 tctttgtttt ccccaactcc tccccaaacc cattccacca gctgtaaatc cttaaccaat
    10621 ctctcggtga ttctaccgga tggcaggaac aggactttct gtaagagccg taaagcgcaa
    10681 taatcatcat tattcaaatc taacttcggg gaatggaagc caatcttcat cttgggagag
    10741 gtctctttat ctttccaaac ataattcttt attctctctt ttttctgggt tggttcaggc
    10801 gaaattgtgt tatctggata agtggctttc ttccatccac cataatactt ttctatcagc
    10861 cggaggagta caggagtctc gaaatttcct gctattacaa tggcacagtg attcggagca
    10921 taaaacctgt ttttgaaatc gatcccgtcc tgcaattgaa tatttttctc caaatcctct
    10981 tcaaatccaa ttaccggatg atgataagta tgcacggtat aagcaagttg ggtgaattcc
    11041 ccctctaaga agccattcgg gtcatccacc tcaaatcttc ttcgctcctc ttttatcgga
    11101 cccagctcag tcctgaaggt ctcgttatca aaaggcaaat ttataatcct atccgcctca
    11161 atctgcacaa tggaatccag aaactgctcc ttggcattga cataatacat agtataatct
    11221 tcgcctgtgg aggcgttggt ctcggcgcca aaaggcgcgg tggcactata atagctggga
    11281 aatttttctg ttccgcgaaa catcatatgt tcaaacacat gcgctaagcc ggtggtgccc
    11341 ggtttgactt cgtcccgtga accggcattt acaaaagtca tataggaaac cgtgggaacg
    11401 gttttgtcct cacaagtaag aacaatcaga ccatttggca gaaccgtttt cttgacacca
    11461 agcttcatct ggtctgtttg ggcaaggcag agttcagaga ggaagaacag tgtgagaaaa
    11521 agcaaggcga ttcttggaaa ccgtttcatc tcttgataac ctcctttttg gttggttggg
    11581 tttgtctaaa gaaaatgttt tcatggccaa ttcgcaatga aacaattatc cttataaaaa
    11641 caactcattc tcccggatcg gacaagtcca gcaggtggtt atgtcacgct gagtcaagcg
    11701 gaattgtgca aaccaccaag catgcctgat gggattttga agaacatctg atttctctcg
    11761 ctttggatag tgaaagctag tcccactcgt ctatctcgcc cttctctttt agcctctcat
    11821 attccgctga atgtttttca tccaattcct ctttgctgat tctgccagtc gtcatgcccc
    11881 aatttatggg atagatttcc gggctgaaga agacgaaata gaaatgccac actatgattg
    11941 ccagagaagc caaaagggct tcataaaaat gaattgattt aaagacatcc aaaagccact
    12001 tggggacaaa tctcaaggag aagtcgggaa accataatac gagaccggtg aatatcatca
    12061 caaaaccgcc ccagaccaaa gcccagtact ccgctttttc accataatta tagcgatcga
    12121 attttggttt tttccttaat agtcccagct tatgaagcac gttttgtatg gcctgcagca
    12181 tatcatttgg cacagggaag atggctttga gttgctcttt gcctcgagcg gtgaaaagga
    12241 tgtaatagag attataaagg caaagggcta tgaaaatcga agcggctatc ctatgcacca
    12301 ttgcgcgcat agccatattg gcggctgatc tggccatcca ggagaaaagg aaaaagtccg
    12361 gaaacctgag cacgaatccg gtgtaagcca aaagggtaaa ggttataaat aaaagaatat
    12421 gctgaagcct ttcggagagc gtgaatctta atatctcttc cttagcgctt tgcctatact
    12481 tatcaatggc atgtctgatc aaatccaata agttatgcag gaacattaaa ccgatcacca
    12541 gaattattaa gaaagtatat aaagaccgta taaaggaagc acctttttcc tcatcgctga
    12601 aatggaactt gcctctggca aagttcactc ctgcaccagt atggcatttg gcacaagtct
    12661 taggcagatt ctcccttgag accgttgacc tggcatcgga tgaaggcctt acgttatgag
    12721 cactatggca atcagaacag tctggcatcc ttttgtcacc agccatgact tcgaccccgt
    12781 gataggggtc gtgataacca gtgagcttct tttcggttac gccaaactgt tttaccacct
    12841 tttcttgcag atgacacccc gaacaggtaa gtttggatat gttggcaggg tagatagtag
    12901 aattcgtatc cccggcagca agaatattat gcgctccgtg acagtcggta caagtggctg
    12961 agcgaagtat tcctctctcc aatgcccttc cgtgaacatc tcctttgtac tcctcgtagg
    13021 aatcgatatg gcatttggag caaagttgag gaatattggc tttagaagcc aaggatttgg
    13081 catctaaagg agattcggtt gcgtgactat gatgacagtc actgcaggtt aatgattctt
    13141 ccatcttctc tttgatgagg agctctccgt gaacgcttcc ggcaaaatca ctcctggcct
    13201 ggggatgaca ccttccgcaa acaccaggaa gattgatacg actcaccttc gattctttgt
    13261 ccgcgatttt tcttatatcg tgtgctccat ggcaatcttg gcaatcagga gcatttttca
    13321 agttctgctc cagtgctttt ccatgaactc cttctttata cgatttcaaa ggggtgggat
    13381 gacatattcc gcaatcgatt ttacctggct tggtctcgtg gacttcgggt cttactccct
    13441 tgtggcaggc aacacagctg agcttggcgt ggacagaatt atccagggta ttcaagcccg
    13501 gctggtcatg gcatactagg cattcttcct tagtctgagc agagacacca gaaaaagagt
    13561 aaatagaaaa aacgaaaagt aaagccaagg tacggaagat tctccccaat ctgtcctcct
    13621 gttactggtg cttccatcct ttttatagag actaaatatc acacatccac tttttcttgt
    13681 caagcccttt tggggcagac aatgtattaa cttgaaacgt actaacctca ttcaccgctt
    13741 tgagtcattc tgtataatct ctttcaacat cagtgcgttc cgaacagctt ttcctatatg
    13801 acaattattg aagaaaacta aggtttgact tgctttggaa tcgaggtcct ttattgggtc
    13861 aacgaatttc tttagttctt cttgcgaata caaatagtca taccttgaac tcttttcagc
    13921 ttcgaaccag tctttattcc ttccatgaaa cctgaaatat gctatgtccg aagttatggc
    13981 tgggttgaaa ggcattagcc cttttaggtt gggttcatct acaatacagt atcctaaatc
    14041 cttgtctttt agaaaatcca ggacttcact cttatgccaa tatctgttcc taaattctat
    14101 aacgattggg attcctaaaa cctgctcttt gaactgtttc agataatcaa agttgtcttt
    14161 ggcaggatag aacgaatatg gaaattgagc aagtatacac aggagttttc ctgaatcgac
    14221 aaaaggttga atggagaatt tgaaattctc gaaactcttt ttgttattct ttttgtcttt
    14281 tatcttgtgt gtcatttcac tgttggcttt aattgagaat ctgaaatcag gagaagtcct
    14341 tttgaccaaa ctttccatag atttacggga aggcagggca taatacgttg aatttatctc
    14401 caagacatta aaacctaaaa ttctttcata atacaaaagt agttggtttt tcttcaggta
    14461 agtgggataa actggtccta tccagctgtc aaaggaaaat ccgcttgttc ctatcttaat
    14521 cattctattt ccccttggtt taagttgcca ctttaaagcc gggaatatgc ttgtgaggcc
    14581 tccaggcggg agagatgaca tttccctcta tcgctggttt gagcagttta gcgtaggtga
    14641 tggtgaactc gccaaactta tcatttatct gatctagggc ataaagcagc ccctcccttc
    14701 ttctttcttc cggaagaata tctagctgct ttgctccttg aattaaattg gagacgctca
    14761 ccccagttaa tctaacccct cgatgttgaa taaaaagcgt atctaagata ccgagggtag
    14821 cttcatagat aagcttccca tcatcaataa atttcttgag ggtatgctgt ttagccacgg
    14881 tttcgaaatc tgcgtatctt aaaattaaag taactgtcct gccctggtaa ttctccctcc
    14941 tcagccttct tccaactttc tcagatagct gcaaaagatg ccttttgact atctctttat
    15001 catgggcgtc ctcctgtaag gtcagagaat gccccataga tttaactggt tctttttcat
    15061 aataatatct tactggactt tcatcttcgc ccaagcccat atggtgaaga tataggccga
    15121 tgattccaaa tcttctttgt aatatctcaa agggatatct gcccagctgt ccgcaggtat
    15181 aaattccaaa tttattgaga tgctgagtca ttttggttcc aatcccacaa agctcggaga
    15241 tgggaacatt ttctaagatt tccaatactt tatctttggt taagacggtt aagccatccg
    15301 gctttttgct ctcactggca aacttagcta aaagcttatt aggtcctatc ccaactgaac
    15361 atgctaaacc caattcattt tttattcttt gctttatctt ttgggctatt tcaacaggag
    15421 tgccaaaaag tctttctgtt ccggttatat ccaaaaatgc ctcatcaatt gagaagactt
    15481 ctacctgggg agtgaaatcg atatagatat tgaataactt ggttgcagta tcaacatatt
    15541 tatccgggtc gcattcaacc agaatagcat ccgggcataa ctttttggct tggtaaatgg
    15601 tcattccggt ctttatacca aattttctag cttcatacga ggcagtggcg accacggtcc
    15661 ttccttttgg attgccacag actaaaatcg gcttccctct aaggtaagga tttgatcttt
    15721 gctccactga ggcaaagaaa gcattcatat caacgtggag tatagttcta tctgacatat
    15781 caaccctcag tttcaatatt agctaatttc cagaccatag attcggtgtt ataacagatc
    15841 tcaaatagat tatcattcgt gtctataact gaaaaatgac atactttcgc tttcccatct
    15901 tgagaaatcc aattgtaggt gatttgagag attttatatt ttctatttcc ccataagaaa
    15961 tagattggct ttaacttctt gccatctcca aagatagcaa caacttttat catctcatct
    16021 atctgggtaa ccatattcct cacctggcat attatactgc acaaatgtgc agttgtcaag
    16081 atatttctat gagagctttg aaaaacttgt ttggcagggc tgtcccgcct atggcgggat
    16141 gcctacgaga ctgcacgaat tccacgaatt ccacgacttc cccgaattct gggttttgag
    16201 atgtgccttg agataactcc ttgatttttc gtcgccagga tccgtaggat aggtcgggca
    16261 ccctgcccca ccaccctctt ccacttttaa cgacaatcag tcaacaccca taaactcgtt
    16321 tgaccccaaa aaaaaatggg cgaccgcaag gccgcccgaa acgaaactcg atatttattt
    16381 cttaagttat taccatacta ctccagccgt gacacccacc gaagcgcctt tggaaggata
    16441 ccagataacc ggcacatcga ttttcaccat actaaagtta aatccaatgc cggtcataat
    16501 gcgaccacaa gcagaagatg gactaatgcc caaagtgctt ccattgactc tgactttccc
    16561 ggctgtttgt gacacggtat ccggcaatcc ctcaactctc acgccccctt tggagctcag
    16621 tacgatatcc gaggagccga agttgaagtc aacacctgcg cccaaattca aattgaaaag
    16681 ccagagaagc tggactgaag tggatatatc cacaggtatt gtggtggtct tcacatcaag
    16741 ccctaagttg aatgacggat caagcaaaag ctgtgcattg gtgcctggat agtaggggtc
    16801 taaaccaact aaatcctctg ctatttcatc ctgctctatt ttaaaatcca ctttactgga
    16861 gtgatacaag agcccggtgc ccaaggataa gcctctccat ttgaaaagtt tctgcccctc
    16921 accaactcct ttaaccagac aatagttggc gccaacgccg aaaacggtgt tcttgatttc
    16981 aacatcctct tgcggcttta agcttaaagt cccgaatttc acattcatat aaagcccggg
    17041 gtgaagagag cttgcattaa aacccaaatt gataaaagat atacccgcgc ccacaccagc
    17101 ataaatatcg ccatctttgt caatgacatc ttcgaggtca ttgtagtaat ccgggtcaag
    17161 gctgggaagc tgaacaccaa ccatagcgcc aacagttacc gcacaatgca ggtaattctg
    17221 gtaaccctga agcgtagcca cttgactgga atagacgttg gcattgccaa aacccttggc
    17281 aaggttttcc attcgcttga atttcgcgag attatcactt gcgtcgtcta tagcaatatt
    17341 gaacgcatca gctaatatgg cgtttatctc ggtatcgtgt ccggagctgc tgactgtggg
    17401 tgcagttccc gatacatcag ccgcctggga atgttgccca tttgctacca caaacaacaa
    17461 ccctatcgcg agcagcatta atgctcttct catagagcct ccttaaaagg tttgagattt
    17521 attttccaag aactaccttc tagtcatcat gtcaaagtgt cataatataa accatttcct
    17581 cctcaagtca actgagaatt tttgacattg tccttttgct tttgttgtaa gttttaactt
    17641 gcaacttcac tgggtatatt ttattttgat gaaattgaat taatctgact aaacagcttc
    17701 aaaaatcaaa cttatatgtc actgccaaaa ttctccagat ttttaggtct tatcagaagc
    17761 tatgattcca ggatgtggat tctcttctcc ggatggatgg tttcatctat gggatttgct
    17821 atggttatcc cttacttgag catctatttc cacaatcaga tgaaaatccc tatggcacaa
    17881 gtaggccttc tctttggaat aggagcaatt gttcgagcac tttctggtct ttacggtggt
    17941 aacctctcag ataaacttgg cagagttaag ataatgggtt gggctcaatt cttaagagga
    18001 gttttctttc tcttgatggg atggcttgcc tggaaagggg gaagttttct tctctggtct
    18061 ttagtgttaa tcctaaactg gctatttggt ggtttttatc agcccgtggc aaatgctatg
    18121 gcagcggaca tggctcccaa ggagaaaagg gtggaagcgt atagcattct gcgcattgca
    18181 gggaatatcg gctgggcttt tggtccaatg attggaggat ttctggcttc tacctcctat
    18241 cctattcttt ttgttttcgc ttccttatca actttcatct cctcttttct tatactgtct
    18301 tttttgccgg aaactattaa aatcaaccac tcagaggaga aattcactct taaggatatg
    18361 ggcaagctaa aatcggacca tctttttctc ctttactgtt ctatttgtct tttgcttttt
    18421 gtggtaattg ggcaattagt ttcaactata tcggttttct cagttgatta tgttggcatt
    18481 tctgaatcta atctgggctt cctctatgcc atcaacggct ttgttgtcat cttttttcag
    18541 ctggctgtta ccaaactgac ccagagaaga aacttaatcc aagtgctctt ttggggaagt
    18601 ttaggttatt ccttgggtta tttcttggtt gggttctcct cgtcttttgc ctggctcttc
    18661 gtctgtatgc ttcttatctc tttttctgag attaccgtat tacccgcctc aatggcgatc
    18721 gtggctaatc tctccaagga aaaacatcac ggcttgtata tgggtgggta tgggcttttc
    18781 tattctctgg gctggtcttt tggacctttg gtaggagggg ttctgattga ccttttgaat
    18841 aataaaccag tgctcttatg gggaataatc gcttccttgg gaatcttatc tgctctgggt
    18901 tttagctgga ttggaaaaaa actgcccagg gaggctatcc ttccgccagc aaaatagtat
    18961 cactagcact gggctcctga cctaccttct aaagaacatc ataattacgg cccagaatat
    19021 aagtgtgcca gcccctaatg caagtatgcc aattataacc cagataagag gtgaaattcc
    19081 cttagaggca gactgttttt ccataatgtt cctccttagc ttttgtttga cttaccagat
    19141 tatatggata gggccctctt aaagttacaa atatgttgcc tgtactttat ggaatggatt
    19201 tcacaattga ttaggttata ttagaagtag tagttgtggt cacgagcccg tgactcttga
    19261 cttactcgta cttggcctgg attctttcta tcctaaatgg aaaaatggaa actctcaagc
    19321 attaatagtg taaaatagag aagatcatct aaatccttta agaaaaggag aaagtaatga
    19381 gaaaaaacct tacatcattc atatcactga tagtgatttt gggtttcgct tgtatggcat
    19441 atgctggcaa tgaagcaacg gttcgctttc cccagaccaa cgctggaaag ctggccgaat
    19501 cctatttcag cgcattcaac agtcataacg aaaatgacat gcggacatat ttggagcaga
    19561 acttcgcacc aaacaccctc aaggagcgcc ccatcgaaga acgcatggcg gtatatcggc
    19621 agctcaagag cgacctggca acattggagc cggtaaaaat tctcaaggag agtacggatg
    19681 cgatcaagct tgttgctcgc tctgcaaagg aggcgtggct tgagcttacc ttcgagttcg
    19741 aaaagaacgc gccttataag ctcctgggca taaggatcat gcgcctggag gaagcacctg
    19801 atcttagtgc tccgacgagt ttgatgaccg aaacggagat gctgaaggag ctcaatactt
    19861 ttatggatgg gatatcagtt aaggacgagt tctctggagt ggttctggtc gccaaaggac
    19921 aaaagccaat cttccgtaag gcttatggct tggcgagcaa agaatataac gtccctaacc
    19981 gcgtggatac gcgctttaat ttgggttcga tcagcaaatt tctgactcgc atcgcgattg
    20041 aacagctcca gggaaaaggc aaactgagtt tggatgaccc gatcggcaaa tatcttccgg
    20101 actatcctaa caaggaagca gctgagaacg taaccatacg ccatctattg gagatgactt
    20161 ccggaatagg agatttcttt ggtga
//
LOCUS       DASWLN010000030        10326 bp    DNA     linear   ENV 20-FEB-2024
DEFINITION  MAG TPA_asm: Candidatus Zixiibacteriota bacterium isolate
            SMAG_U2949 k141_141302, whole genome shotgun sequence.
ACCESSION   DASWLN010000030 DASWLN010000000
VERSION     DASWLN010000030.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37960594
            Sequence Read Archive: SRR10376091
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Candidatus Zixiibacteriota bacterium (soil metagenome)
  ORGANISM  Candidatus Zixiibacteriota bacterium
            Bacteria.
REFERENCE   1  (bases 1 to 10326)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 10326)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (26-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/12/2024 08:28:26
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 1,440
            CDSs (total)                      :: 1,416
            Genes (coding)                    :: 1,412
            CDSs (with protein)               :: 1,412
            Genes (RNA)                       :: 24
            rRNAs                             :: 1 (16S)
            partial rRNAs                     :: 1 (16S)
            tRNAs                             :: 21
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 4
            CDSs (without protein)            :: 4
            Pseudo Genes (ambiguous residues) :: 0 of 4
            Pseudo Genes (frameshifted)       :: 0 of 4
            Pseudo Genes (incomplete)         :: 3 of 4
            Pseudo Genes (internal stop)      :: 1 of 4
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..10326
                     /organism="Candidatus Zixiibacteriota bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_141302"
                     /isolate="SMAG_U2949"
                     /isolation_source="soil"
                     /db_xref="taxon:2053527"
                     /environmental_sample
                     /country="USA: Oregon"
                     /lat_lon="42.99 N 123.63 W"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            complement(<1..638)
                     /locus_tag="VGB16_01060"
     CDS             complement(<1..638)
                     /locus_tag="VGB16_01060"
                     /inference="COORDINATES: protein motif:HMM:NF012497.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEX9916299.1"
                     /translation="MSRIIKPIKKINGKLTLPGDQFASHLSLVLASLSQGESVLHNLS
                     PGKDCQITIQCLRDLGIDIQIDSNRARVLGKGLSGITPPKGVIDFDDSEITLYLVAGI
                     LSGLEGEFQLKGDVSLGEKFRQELYPPLEKMGVEVTPSKRKEAVITLKGRGLKTLNFH
                     LNQESSLAKISLQLTSLFAKGYTNFIQAEKTENETEKPLELFGANLKTGYEQ"
     gene            complement(641..2338)
                     /gene="argS"
                     /locus_tag="VGB16_01065"
     CDS             complement(641..2338)
                     /gene="argS"
                     /locus_tag="VGB16_01065"
                     /EC_number="6.1.1.19"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_007220209.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0005737 - cytoplasm [Evidence IEA];
                     GO_function: GO:0004814 - arginine-tRNA ligase activity
                     [Evidence IEA];
                     GO_process: GO:0006420 - arginyl-tRNA aminoacylation
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="arginine--tRNA ligase"
                     /protein_id="HEX9916300.1"
                     /translation="MNEFIQEVTGILGGKIGLSQKELEGLIEIPPDQSLGDYAFPCFV
                     LAKKLKKSPQEIALELASELKPQGLIEKITSSGPYLNFGVKKDKLAEFVLKKINKEKD
                     SYGSDKIGKGKTIVIDFSSPNIAKPFGIGHLRSTVIGNALYKIFTKLGFKVIRVNHLG
                     DWGTQFGKLIVAYKRWGDEKEISSDPIASLFNLYVRFHDEAEKDKTLEDEAREHFRKL
                     EKGDREATSLWKRFKKYSMDEFERIYKMLDVSFDYYAGESFYNELVDQTVEKIQKSGL
                     AEISQDALVIKLDKYNLPPCLIRKKDEATLYATRDITAAIYRYDTYKFFKNIYVVGTA
                     QKLHFQQVFKVLELLGYEWTKDMVHVDFGWVKFKQEMMSTRKGNIILLEDVLNKSIEL
                     TLKIIEEKNPGLESKKEVAYQVGIGAVIFADLSVRRNKDIDFDWERALSFEGETGPYL
                     QYTHARLCSLVSKYGKSANDKVDYSTFQSTEEIALIKLLDSFPQKIKQAADTYEPYLI
                     SSYLIELCSLFNRFYQRERVITEDDQKTKAKMLLVSGMLVVLALGLDLLGIKAPKRM"
     gene            complement(2340..3614)
                     /locus_tag="VGB16_01070"
     CDS             complement(2340..3614)
                     /locus_tag="VGB16_01070"
                     /inference="COORDINATES: protein motif:HMM:NF019310.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0022857 - transmembrane transporter
                     activity [Evidence IEA];
                     GO_process: GO:0055085 - transmembrane transport [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="MFS transporter"
                     /protein_id="HEX9916301.1"
                     /translation="MIKKLNSHISDYRRNLSLFSTNARLFLIGNFFFGLGFSIYQLLL
                     NLYFKEMGFSESLIGQVLSAISLGMMLICVPAAMAIQKISIKRILVGSTLLAGLFYFL
                     QAIITGKQMLLLVSFMVGVTMSFSRVAIAPFLMRNSTPEERTHLFSLNFGTTLLAGTI
                     GSLGGGYLTSLWSKISGSQVLGFRYALFSGVFLGLLGIIPFSLIKKTDRDQLSTTNGF
                     DLNRNLLRERGNVLLKLLLPYFILGLGAGLVIPFLNLYFRDRFNLSAGTIGIYFSLLQ
                     FVMLTGILIGPILSRKSGMIKTIVYTQLASIPFMLILAFTFNNTLAVLAFLLRGSLMN
                     MSQPISTNFTMEKVKEDEQPLTNSLMMLAWTSSWAIVASLGGNLISRHGFTLPLLIAV
                     VLYVISSLLYLKFFSGKEDIKMGKITTILRQA"
     gene            complement(3630..4469)
                     /gene="dapF"
                     /locus_tag="VGB16_01075"
     CDS             complement(3630..4469)
                     /gene="dapF"
                     /locus_tag="VGB16_01075"
                     /EC_number="5.1.1.7"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_018205196.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008837 - diaminopimelate epimerase
                     activity [Evidence IEA];
                     GO_process: GO:0009089 - lysine biosynthetic process via
                     diaminopimelate [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="diaminopimelate epimerase"
                     /protein_id="HEX9916302.1"
                     /translation="MKIEFVKMHGLGNDYILINSLKGNLGRTNIKELPKNICHRNLGI
                     GADGIILALSGKNHPFQMRIYNSDGTEAEMCGNGIRCFARYLYECGLSRKRKQKIETP
                     AGLIQTEITNPKRNFLVKVNMGKPILVRNVIPVKGSKNFCINENLKVNGKVFNFTAVS
                     MGNPHAVVFVKEFDNNWQKWGKMIENHSLFPKKTNVEFVKITDRKRIELRVWERGAGS
                     TLACGTGACAAVVAGILNGNLDRKVKVDFPFGQLLIEWSKKDNCVFMTGPAEKVCEGI
                     YDF"
     gene            complement(4601..5788)
                     /locus_tag="VGB16_01080"
     CDS             complement(4601..5788)
                     /locus_tag="VGB16_01080"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_018154442.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0030170 - pyridoxal phosphate binding
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="PLP-dependent aminotransferase family protein"
                     /protein_id="HEX9916303.1"
                     /translation="MSTIWNFSTRAKEVKSSVIREILKITERPDIISFAGGLPAPELF
                     PVEEIKKACIKVLDESGSKALQYSLTMGHLPLRRAIAERISKRDGIKVTEENIVITGG
                     SQQGLDIMGKLFLDSTSTLIIEKPSYLGAIQAFNSYRPTYITVGMDNEGMLLDEMEED
                     IKKHKPRFIYAVANFQNPSGITMSLKRRKRLVELSKEFYTPIVDDNPYGEIRYQGKDL
                     PSLKSLGGDNVIELGTCSKIISPGLRIGWIVGPADVMLMIEKLKQGIDLHTNTFAQYV
                     VYEYIKTGQLDEHITKIKKAYGERIKVMLEALETYFTKEIKWTKPEGGLFLWVELPQG
                     NSATDILPKAVAQKVAYVPGKPFFVDESGDNTLRLNFSNATPEMIVEGIKRLGKVFKE
                     NIK"
     gene            complement(5874..6353)
                     /locus_tag="VGB16_01085"
     CDS             complement(5874..6353)
                     /locus_tag="VGB16_01085"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEX9916304.1"
                     /translation="MRWKEKLKKLKEEAKSRKIEQKLEHQILAEKVTEGIEECLFVLE
                     EFSSITNSEIKFIDNRHMEKDEQTGKKYFAGGITLIVERRKGLPEKIFRLPEKRSRII
                     ALVKFPHPKAIRNMEWYEDSVKFIYSDIARKLPLPQFNSQWLKDNLEEAYQYFLNQQ"
     gene            6529..7065
                     /locus_tag="VGB16_01090"
     CDS             6529..7065
                     /locus_tag="VGB16_01090"
                     /inference="COORDINATES: protein motif:HMM:NF015871.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="OmpH family outer membrane protein"
                     /protein_id="HEX9916305.1"
                     /translation="MSIRKPLIMLVISLIILGFVSSVLAQGLKIGYVDTNHLKGEYKE
                     FADAQAKFETELNAWQNKADSLQADILKLQNDLTSQGMLLSEAAKKEKEALLKKKIAE
                     FDQFRTKILGPTGEAAKREKELSQPLVDKITKVIEKIAQRDGYTYVLDSSGGEVLFAP
                     DSLDLTEKVLQELQPVTK"
     gene            complement(7842..8696)
                     /locus_tag="VGB16_01095"
     CDS             complement(7842..8696)
                     /locus_tag="VGB16_01095"
                     /inference="COORDINATES: protein motif:HMM:NF013245.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0042626 - ATPase-coupled transmembrane
                     transporter activity [Evidence IEA];
                     GO_function: GO:0140359 - ABC-type transporter activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="ABC transporter permease"
                     /protein_id="HEX9916306.1"
                     /translation="MIERLKNDLRTIKLAGWLGWAIESNWADPFIFAIYSIIRPIASS
                     LILVFMYMVVSRGNTSTEFFAYIFTGNAFFMYVVNVLFGIAWVIHDDREHYQMIRYVY
                     ISPIRMYYYFFGRGIAKMIVTTIAVIVTMSFGIIVLKIPLDLGAIDYPLLLFSLAMGI
                     FIITCLGILLAGITMVTAHHSFLLTEGVAGLFYLACGAIYPIDILPIWLQYLSKTLPL
                     TYWLELIRRAVLGKSLSLTLGEFSDAQLILIMLVTTLILALFSHYVYRSIEHIARKHG
                     KIDQMMNY"
     gene            complement(8698..9516)
                     /locus_tag="VGB16_01100"
     CDS             complement(8698..9516)
                     /locus_tag="VGB16_01100"
                     /inference="COORDINATES: protein motif:HMM:NF013245.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0042626 - ATPase-coupled transmembrane
                     transporter activity [Evidence IEA];
                     GO_function: GO:0140359 - ABC-type transporter activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="ABC transporter permease"
                     /protein_id="HEX9916307.1"
                     /translation="MKTSLKTNLRAVLARSYVRFWAANREPSWWVSETIMPLLSTAAY
                     VFVYKALKAPEEYIGFVILGGTMSAYWMNVLWSMAAQFYWEKEMGNLELYMIAPISRM
                     SILFGMASGGFIHASTRAVAVFLMGSLIFKVDFAVISVTKLIIVFVLTLVALYGLGML
                     FSSVFMLYGREAWHGANLLQEPVYLLSGFYFPVRNLGFAVALVASFIPLTLGLDAMRQ
                     LAFRAGSKSGFINVNLELSVLVLLCVIFLVAANYALKYMEYLGKKEGRLTLRWQ"
     gene            complement(9488..>10326)
                     /locus_tag="VGB16_01105"
     CDS             complement(9488..>10326)
                     /locus_tag="VGB16_01105"
                     /inference="COORDINATES: protein motif:HMM:NF012235.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005524 - ATP binding [Evidence IEA]"
                     /codon_start=3
                     /transl_table=11
                     /product="ATP-binding cassette domain-containing protein"
                     /protein_id="HEX9916308.1"
                     /translation="LATLLLPSSGTALVNGVDVVKHPEEVRKYINMVSGGEHSGYGIL
                     TVRETLWMFAQFYGIPTGVALKRIEGLAKILDMEKFMKTKIGKLSSGMRQKLNVMRGF
                     VCDPKILFLDEPTLGLDVQISREVRKYIKKWVKENQDKTVLLTTHYMAEADELCDRVA
                     IIDQGKVLVCDTPHNLKKLLQKEVIFHIETSLMLNHMESFSNLKGIKNFAYQHQSDMG
                     RTKIKLILEDESTISDVTEIIDENNSKILSLTKVEPTLEDVFVSLVGRVLENENQSEN
                     QS"
BASE COUNT         2947 a         2356 c         1998 g         3025 t
ORIGIN      
        1 tcttgctcgt agccggtttt taagttagct ccaaaaagct ccaatggttt ttcggtctca
       61 ttttccgttt tttcagcttg aatgaagttg gtataccctt tggcaaaaag agaggttagc
      121 tgtaagctga ttttcgccaa agagctttcc tggttcagat gaaagttcaa ggttttcaat
      181 cctcgacctt ttaaggtgat gaccgcctct tttcttttgg agggagttac ttcgactccc
      241 attttttcca gtggggggta gagttcttgt ctgaattttt cacctaaaga gacgtctcct
      301 ttgagttgaa actcaccttc taaaccagaa aggattccag ccacaagata aagtgtaatc
      361 tctgaatcat caaaatctat tactcccttc ggaggagtga ttccgcttag tcctttgcct
      421 aaaactcttg ctctattcga atcaatttga atatcaatcc ccaaatctct taaacattgg
      481 atagtgattt gacaatcttt gccaggtgaa agattgtgta atacactttc cccctgactt
      541 aaactcgcca aaaccaagct gagatgtgaa gcgaattggt cccctggcag agtcaatttc
      601 ccatttatct tttttattgg cttgatgatt cttgacatat ctacattctc ttgggcgctt
      661 ttatgcccaa caggtccaac cccagagcca gcacaaccag catcccactg accagaagca
      721 tcttggcttt ggtcttttgg tcatcttcgg ttatgacccg ctccctttga taaaagcgat
      781 tgaaaagtga gcaaagctct atcaaataag aggagatgag atatggttca taagtgtcag
      841 cggcttgttt tattttttgc gggaaggaat ccaacaattt gattaaggcg atttcctccg
      901 tggattgaaa agtgctgtaa tccaccttat cgtttgcaga ttttccatac ttggagacta
      961 aactgcacaa gcgggcgtgg gtatattgta gatatggtcc ggtctcacct tcaaaactta
     1021 aggccctctc ccagtcgaaa tctatatctt tatttcttct cacgctcaaa tcagcgaata
     1081 tcactgcacc gatgccaacc tgatacgcca cctccttctt gctctccaat ccaggatttt
     1141 tctcttcaat gatttttaag gtaagctcaa tggatttatt caaaacatcc tctaacagaa
     1201 ttatattccc cttgcgagtg gacatcatct cctgtttgaa tttgacccag ccgaagtcaa
     1261 cgtgaaccat atcctttgtc cattcgtatc ccaatagttc caaaaccttg aacacctgct
     1321 ggaaatggag cttctgggct gttcccacca catagatgtt cttgaagaat ttataagtat
     1381 cataacgata aatagcggca gttatatcgc gggtagcgta taaagtggct tcatcttttt
     1441 ttcttattaa gcaaggcggc aggttgtatt tgtccaattt aattaccaag gcatcctggc
     1501 ttatttccgc caaacccgat ttttgtatct tctcaactgt ctggtcaact agttcattat
     1561 agaagctttc accggcatag taatcgaaag atacatctaa catcttatag attctttcga
     1621 attcatccat gctgtatttt ttaaatctct tccagagcga agtcgcctct ctgtcacctt
     1681 tttccagttt cctgaaatgt tcccttgcct catcttctaa agttttatcc ttttcagcct
     1741 catcgtgaaa tctaacgtaa aggttaaata agctggcaat cgggtcggag ctaatctctt
     1801 tctcatcgcc ccatctttta taagccacga tcagtttccc gaattgtgtt ccccagtctc
     1861 ccagatgatt tactctaatt accttaaagc ctagctttgt gaaaatctta tataaagcgt
     1921 ttccaattac agttgagcgg aggtgcccta ttccaaacgg cttggcaata ttaggggagg
     1981 agaagtctat aacaatggtt tttccttttc ctattttgtc tgagccgtag ctatccttct
     2041 ccttgttgat tttctttaaa acaaactcag ccaacttatc tttcttgact ccgaaattga
     2101 gatatggtcc ggaagaagtg attttctcga ttaggccctg gggtttcagt tccgaagcca
     2161 attcaagcgc aatctcttgg ggtgatttct ttagtttctt ggccagaaca aaacagggga
     2221 aagcatagtc ccccagagat tggtcaggcg ggatttcaat taacccttcg agctcttttt
     2281 gggacaagcc aatttttccc cccaagatgc ctgttacttc ttgaataaat tcattcatct
     2341 taagcctgcc gcaggattgt ggttattttc cccattttga tatcttcctt accggagaaa
     2401 aatttgagat ataataagct ggaaatgaca tacaatacca cagcaataag cagtggaagg
     2461 gtgaatccat ggcgactgat tagatttcct cccaagctgg caacaattgc ccaggagctg
     2521 gtccaagcta gcatcattag actgttggtc aaaggctgct catcttcttt gactttttcc
     2581 atggtgaagt tggtggaaat cggctggctc atattcatca gggatcccct caaaagaaaa
     2641 gccaataccg ccaaggtgtt gttaaaagtg aaagccaata tgagcatgaa cggtatggaa
     2701 gccaactgag tataaacgat agttttaatc attcccgatt tcctggatag gataggtcca
     2761 attaggattc cagttaacat gacaaattgc agtaacgaaa aatagattcc aatagtcccg
     2821 gcggaaaggt taaatctatc cctgaaatac aggtttaaaa atggaatcac cagaccagca
     2881 cctaaaccca ggatgaaata aggtaaaagc agttttaaaa ggacattgcc tctttctctt
     2941 agtagatttc tgttcaaatc aaatccgttt gtggtgctca attggtctct gtccgttttc
     3001 ttgatcaaag agaagggaat tattcctaat aaccccagga agacaccact aaaaagagca
     3061 taacgaaaac ccaaaacctg tgacccggat atcttcgacc atagactggt gagatatcct
     3121 ccacctaaac taccgatagt gccagccaaa agcgtagttc caaaattcag actaaaaaga
     3181 tgagttctct cctccggggt gctgttgcgc ataaggaaag gtgcaatcgc aactcgagag
     3241 aaagacatgg ttactcccac cataaagcta accaaaagaa gcatttgttt tcctgttatg
     3301 atcgcctgca aaaagtaaaa taacccagcc aaaagggtgg aacccacaag gatcctcttt
     3361 atgcttatct tttgaatcgc cattgccgca gggacacaga taagcatcat tcccaaagag
     3421 atagcggaca gtacctgccc gatcaagctt tccgagaatc ccatctcttt gaaatataaa
     3481 ttcagcaaaa gctgataaat agaaaagcct aacccaaaaa agaaattccc aattagaaac
     3541 aatcgggcat tagttgagaa aagggaaagg ttccgccgat agtcggatat atgagaattc
     3601 agctttttta tcaatttgaa cgagcaagtt taaaaatcat agattccctc acagactttc
     3661 tctgcaggtc cggtcataaa tacacagtta tctttcttac tccattctat gagcaattgg
     3721 ccaaaaggga aatccacctt caccttgcgg tctaagtttc catttagtat tcccgctacc
     3781 actgcggcac aggcaccagt gccacaagcc aaggtggaac cagcccctct ttcccaaacc
     3841 cgaagctcga ttctctttcg gtcggtaatc ttgacaaatt caacgttggt cttcttagga
     3901 aaaagagagt gattttctat catttttccc catttctgcc aattattgtc gaactcttta
     3961 acgaagacga cagcgtgggg atttcccata gagaccgcag taaaattgaa aactttccca
     4021 tttactttca agttttcatt tatacagaag tttttacttc ctttaaccgg aattacattt
     4081 ctcaccaaaa taggtttgcc catattcacc tttaccagaa aattcctctt tgggtttgtg
     4141 atttcggttt gaatcaatcc ggctggtgtc tctatttttt gttttctttt tctggataat
     4201 ccgcattcat ataaataccg agcaaaacat cttattccat ttccacacat ctcagcttcg
     4261 gtcccatccg aattgtaaat tctcatctga aaaggatgat tttttccaga taatgccaag
     4321 atgataccat ctgcgcctat gcctaaattt cgatgacaga tatttttagg cagctctttg
     4381 atgttggtcc ttccgagatt tcctttcagt gaattgatga ggatatagtc atttcccaaa
     4441 ccgtgcattt tgacgaattc tattttcatc tctctccctt ttggcaaaat ctaaattaaa
     4501 aagtcatttt tagcaactgt ttttaataaa aaagtcccgc cacaggcggg acttaaaaca
     4561 taaacataaa gccctatact ttattgtctc aatagaaaaa ctactttatg ttttccttga
     4621 agaccttgcc tagtctctta attccctcga ctatcatctc tggtgtcgca ttagaaaaat
     4681 ttagtcttaa ggtgttatcg ccgctttcat ccacaaagaa cggtttgcct ggaacataag
     4741 ccaccttttg agccactgcc ttaggcagga tgtcagtggc agaattgcct tggggtagct
     4801 cgacccagag gaaaagtcca ccttcgggct tagtccattt gatttctttt gtgaaatatg
     4861 tttccaaggc ttcaagcata acctttattc tctcaccata agctttcttt atcttggtaa
     4921 tgtgctcatc taactggcca gttttgatat actcatacac cacatactga gcaaaagtgt
     4981 tagtgtgcag atcaattcct tgctttagct tctcaatcat caacatgaca tcagctgggc
     5041 ccacaatcca gccaatcctc aagcccggcg agatgatttt ggagcaagtt cccaattcta
     5101 tgacattgtc tccccccaag gacttcaacg aaggtaaatc cttgccttga tatcttatct
     5161 ctccataagg attatcatct actataggag tatagaattc cttactcaat tccaccaaac
     5221 gtttgcgtct tttcaggctc atagttattc ctgaagggtt ctggaaattg gcaaccgcat
     5281 agatgaatcg cggcttatgc ttttttatat cctcctccat ttcatctaag agcatcccct
     5341 cgttgtccat ccccacagtt atgtaagttg gtcggtatga attgaaggct tgaattgctc
     5401 ccaagtaaga tggcttttca attatgagcg tgctggtgga atcaaggaaa agttttccca
     5461 ttatgtccaa accctgctgg gaacccccgg ttataactat attctcttca gttaccttaa
     5521 tcccatctct tttggaaatg cgctcggcta tggctctacg taagggaaga tgacccatag
     5581 tcaagctata ctgcaatgcc ttcgagcccg attcatccaa aaccttgata cacgcctttt
     5641 ttatctcctc aactggaaac agctcaggcg caggaagccc accggcgaag gagatgatgt
     5701 ctggtcgttc ggttatttta agtatctcac ggataacaga gctttttacc tccttggctc
     5761 gagtggaaaa attccatata gtagacaaat ttcctccttt gtgaaacttt taactatctc
     5821 tttgtcaaaa ataaatacaa aaaattaaaa taaagtcaag gaaaatcgtt ctcttattgt
     5881 tgattgagga aatattgata agcctcttcc aggttatctt tcaaccattg ggaattgaat
     5941 tgaggcaaag gtagcttcct tgctatatca gaatagataa acttcacgga atcttcgtac
     6001 cattccatat ttcttatggc tttgggatgg ggaaacttta ctaaagcgat gatacgactc
     6061 cttttttccg gcaagcgaaa aatcttttca ggtaatccct ttcttctttc tactattaag
     6121 gttatcccgc cagcaaagta tttcttgccg gtctgttcat ctttctccat atgccgatta
     6181 tcaataaatt tgatctcgct attggtaatt gaagaaaact cttccagaac gaacaagcac
     6241 tcttcgatgc cttcagtcac cttttcagcc agtatctgat gctccagttt ctgttcaatt
     6301 ttcctgcttt tagcttcctc tttcagtttc tttagtttct ctttccatct cataagaatg
     6361 ccttttagag aatataaaca tccactaatc gcattgtcaa gttgaaatga ctatgaaagt
     6421 tgtgttggca tcggaaattt tcttcttatt ttgtgcttga caatagttgg ataggagtta
     6481 tctttgtagc acaaaatcca ttttcaaatc tatctaggag gtttattgat gtcaattagg
     6541 aagcctttga ttatgttagt gataagtctg attattctcg gattcgtctc ttcggttttg
     6601 gctcaaggat tgaaaatagg gtatgttgat accaatcacc tcaaagggga gtataaagaa
     6661 tttgccgatg cgcaggcgaa atttgaaacg gagcttaatg cctggcaaaa caaagctgac
     6721 tccctgcagg cggatatact gaaattacag aatgatttaa cgagtcaagg tatgctccta
     6781 agtgaagccg ccaagaaaga gaaagaggcg cttctaaaga agaagatagc ggagtttgac
     6841 cagtttcgca ccaagatatt aggtccgact ggagaagctg ccaagaggga aaaggaactg
     6901 agccaacccc tggttgacaa gataacaaaa gttattgaaa agattgccca aagagatggg
     6961 tacacgtatg ttctggattc ttcaggcggt gaggttttgt ttgccccgga ctctttggat
     7021 ctaacggaga aagttttgca ggaattgcaa ccagtaacca agtaagtttt attgcaagat
     7081 agatcaaaag ggatgagctt tgaaaagctt atcctttttt attttggtta aaaactagaa
     7141 actgtatgaa agggttcgag tcaccgggat tccgctaaaa gcggaaaaat ccagttcccg
     7201 aactaactaa tacaaacgca ttaacttacg ttcaataaat ctcccctggc cctcgcctcg
     7261 attcggtccg cctcggcgag ccatcggcga gacgggcgaa gcgggcctct tttccaaaga
     7321 gggaaaaaaa gaggtcttct ccccctttga aaaaggggga tttgatggtt cgatatgggc
     7381 ttcgactgag tccttcgacg gtgagccgct caggacggtc gaaccgctca gccgaatgtc
     7441 ctcaccatct cttcgagcct gagccttcct tcgacaccga ggctcaggac gaagtgcaga
     7501 gccgaaggcc tgagcaaagt cgaagggagg gggattttaa aatagattgt atgttgctct
     7561 aatatgtaac aacatttaat gcgtctgcta tagaaccaat cggagatggt gttcgcgtag
     7621 aggtggagcc ccgcactagc tcactgcggg ataaatttat ctccacccgc atccttcggg
     7681 cgaaggcggg cttcgcccct acgggtttct attattgcta caaatgcaac taattggagt
     7741 gcaaaccttc acgaattctg tgtcttgaga taggtttgca aaatcttcat aaagctgaag
     7801 ctttatactc ctcaagactc cccgcaatgg cacttcatat tttaataatt catcatttgg
     7861 tcaatcttcc cgtgctttcg tgcaatatgt tcaattgaac gataaacgta atgtgagaac
     7921 aaagccaaaa tcaaggtcgt aaccaacatg attagaatca actgcgcatc cgagaactca
     7981 cccagagtca agcttaagct ttttcccagt actgctcttc taatcaactc caaccaatag
     8041 gtcagaggga gggttttaga taagtattga agccaaatcg gcaaaatatc aatgggataa
     8101 attgccccac aagccagata aaataatcct gcgacccctt cggtcaagag aaaactgtga
     8161 tgcgctgtca ccatagttat gcctgccaag agtattccca agcaggtgat gatgaatatt
     8221 cccatagcca gactgaaaag aagcaggggg taatctatgg cacccagatc caatggtatc
     8281 ttcaagacaa taattccgaa gctcatagtt acaatgaccg caatggtggt gactatcatc
     8341 ttggcaatcc cccgaccaaa gaaatagtaa tacattctaa tcggagagat atagacgtat
     8401 cttatcatct ggtagtgttc gcggtcatca tgaatgaccc aggcaattcc aaagagaaca
     8461 tttaccacat acataaagaa ggcattgccg gtaaagatgt aagcgaaaaa ctcggttgaa
     8521 gtgtttccgc gcgagaccac catatacata aagaccagaa tcaaagaaga ggcaattggc
     8581 cgaataatag aatagatagc gaagataaac ggatctgccc aattgctctc tatagcccaa
     8641 cccagccagc cagctagctt tatagtcctt aaatcgtttt tgagtctctc tatcatttta
     8701 ttgccatctc aaggttagcc ttccctcttt cttgcctaag tattccatat atttcaaggc
     8761 gtaattcgct gctactaaaa atatcacaca caaaagcaca agcacactta gttccagatt
     8821 gacattgata aatccggatt tgcttccagc ccggaaagct aattgcctca ttgcatctaa
     8881 gcccaaagtc aaaggaataa aggaagcgac cagggcaact gcgaagccca gattcctaac
     8941 cgggaaataa aatccagaca aaaggtaaac tggttcctgg aggagattgg ctccgtgcca
     9001 cgcctccctt ccatacaaca tgaagaccga tgagaaaagc attcccaggc cgtagagggc
     9061 cactaaggtc agaacgaata ctataatcag tttagtgaca ctgattacag caaagtccac
     9121 cttaaatatc aaacttccca ttagaaaaac tgctactgct cgagtgctgg cgtggataaa
     9181 acctccggat gccattccaa aaagtatgga catacgggag ataggagcaa tcatgtaaag
     9241 ctccaaattc cccatctcct tttcccaata aaattgcgct gccatagacc aaaggacatt
     9301 catccagtaa gcgctcattg ttccacccag gatgacaaag ccgatatatt cctccggtgc
     9361 cttgagagcc ttatacacga agacataagc cgcagtgctc aaaagaggca taatcgtttc
     9421 gctcacccac cagctcggct cccgattagc tgcccagaag cgaacatagg aacgggcaag
     9481 aactgcccta agattggttt tcagactggt tttcattttc cagaactctt cccactaaag
     9541 agacgaagac atcctctaag gttggctcaa ctttagtcaa agagagaatc ttcgagttat
     9601 tctcgtcgat tatctcagtc acgtcggaga tggtcgattc atcctccaat atcagcttga
     9661 tttttgttct tcccatatcg ctttgatgtt gataagcgaa gttcttaatc cctttgagat
     9721 tcgaaaagct ttccatatgg ttgagcatca aactggtctc aatatgaaag atgacttcct
     9781 tttgcagaag ctttttcaga ttgtgaggag tatcgcaaac taagactttt ccctgatcaa
     9841 ttatggctac cctgtcacaa agctcgtccg cttcggccat atagtgagtg gttagaagaa
     9901 cagtcttgtc ttggttttcc ttgacccatt tcttaatata ctttcgcacc tcacgagaaa
     9961 tctgaacatc cagacccaag gtcggctcat ccagaaaaag tatcttgggg tcgcatacga
    10021 agcccctcat cacattcaac ttttgtctca ttccagatga gagttttccg attttggtct
    10081 tcatgaattt ctccatatcc aagatcttgg ccaatccctc gattctcttt agggcaactc
    10141 ctgtgggtat gccgtagaat tgagcaaaca tccaaagagt ttcccttacc gttaaaatcc
    10201 cgtagccgga gtgctctcca ccagaaacca tattgatata tttgcgcacc tcctctggat
    10261 gtttcactac atccactcca ttgactagcg cagttcctga agaaggaaga agaagggttg
    10321 ccagga
//
LOCUS       DASWLN010000031         6874 bp    DNA     linear   ENV 20-FEB-2024
DEFINITION  MAG TPA_asm: Candidatus Zixiibacteriota bacterium isolate
            SMAG_U2949 k141_920874, whole genome shotgun sequence.
ACCESSION   DASWLN010000031 DASWLN010000000
VERSION     DASWLN010000031.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37960594
            Sequence Read Archive: SRR10376091
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Candidatus Zixiibacteriota bacterium (soil metagenome)
  ORGANISM  Candidatus Zixiibacteriota bacterium
            Bacteria.
REFERENCE   1  (bases 1 to 6874)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 6874)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (26-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/12/2024 08:28:26
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 1,440
            CDSs (total)                      :: 1,416
            Genes (coding)                    :: 1,412
            CDSs (with protein)               :: 1,412
            Genes (RNA)                       :: 24
            rRNAs                             :: 1 (16S)
            partial rRNAs                     :: 1 (16S)
            tRNAs                             :: 21
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 4
            CDSs (without protein)            :: 4
            Pseudo Genes (ambiguous residues) :: 0 of 4
            Pseudo Genes (frameshifted)       :: 0 of 4
            Pseudo Genes (incomplete)         :: 3 of 4
            Pseudo Genes (internal stop)      :: 1 of 4
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..6874
                     /organism="Candidatus Zixiibacteriota bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_920874"
                     /isolate="SMAG_U2949"
                     /isolation_source="soil"
                     /db_xref="taxon:2053527"
                     /environmental_sample
                     /country="USA: Oregon"
                     /lat_lon="42.99 N 123.63 W"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            complement(<1..1356)
                     /locus_tag="VGB16_01110"
     CDS             complement(<1..1356)
                     /locus_tag="VGB16_01110"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_013629103.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_function: GO:0016887 - ATP hydrolysis activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="ATP-dependent Clp protease ATP-binding subunit"
                     /protein_id="HEX9916309.1"
                     /translation="MNEMFTEKARKAIEYARDEAARLRHDYIGTEHLLLGLVRLGEGK
                     AIEIMANIGIDLNELRQSIEDVVQPSGGTMTMGQLPLTARAKKTLEISGQEARALRSN
                     EIDTEHLLLALLRDEEGVAAQVLSMYDIDYKEVYEELKNIQSGKPSAYGKKRKKSKTP
                     ALDHFGRDLTELARKGKLDPIIGRENEIERLSQILSRRKKNNPVLIGEPGVGKTAIVE
                     GLAEKIVENKVPQILENKRIVSLDMASLVAGTKYRGQFEERMKAVMTEVANSKDVIIF
                     IDELHTIVGAGGAEGSLDASNIFKPSLSRGDMQCIGATTLNDYRKYIEKDGALERRFQ
                     TVMIDPPTLGDTVKILQGLKSRYEEHHKIKISDEAVEAAVNLSDRYISGKFQPDKAID
                     VIDEAGSKAHLATCTKPDVFGKLEEEIIHLQKQKEQAVKNQEFEKAAQLRDELKFKKE
                     EL"
     gene            complement(1408..2460)
                     /locus_tag="VGB16_01115"
     CDS             complement(1408..2460)
                     /locus_tag="VGB16_01115"
                     /EC_number="2.7.14.1"
                     /inference="COORDINATES: protein motif:HMM:NF002194.0"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016301 - kinase activity [Evidence IEA];
                     GO_process: GO:0046314 - phosphocreatine biosynthetic
                     process [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="protein arginine kinase"
                     /protein_id="HEX9916310.1"
                     /translation="MFEEIVKKPASWLAGDGTQSGIVLSSRIRLARNISGCLFTSVAD
                     AQNRERVITFAKLAIDKSPNLKKGTFLLWNELSELDKRLLMERHLISPEFVRGNDSRA
                     LFVSDEEMVSIMINEEDHLRIQIIQSGLEIRDTCCIADKIDDDLGQSLEFDFDADFGY
                     LTSCPTNVGTGMRASILIHLPALVLIREMEKVISQITKVGLTVRGFYGEGTDVLGNLF
                     QISNQTSLGRSEEDIIESLEKITKQIIEYETNARQTLFQDAKEEIEDKIWRAYGILKS
                     ARVLTSEEVMNLLSAIRLGLGMGTIDLLPLPLINEILILSQPAHLQKYFNKDMNEDER
                     DIMRAELVRSKLANYN"
     gene            complement(2463..2978)
                     /locus_tag="VGB16_01120"
     CDS             complement(2463..2978)
                     /locus_tag="VGB16_01120"
                     /inference="COORDINATES: protein motif:HMM:NF014232.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005515 - protein binding [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="UvrB/UvrC motif-containing protein"
                     /protein_id="HEX9916311.1"
                     /translation="MLCEDCKKNKATIQWTQVINDEKMMLNLCKDCAEKRGFHSPFEE
                     VPFPLAEFLSSMLDKGVKKEGIALAEVKCPNCQMTFAEFSRVGRLGCGSCYTTFRVQL
                     NDLLRKIHGSNRHRGKIPYFPGDSMKPLREERKLQEELKRAVEQEDFEEAARIRDRIK
                     TLIKEKNVEGK"
     gene            complement(2989..3681)
                     /locus_tag="VGB16_01125"
     CDS             complement(2989..3681)
                     /locus_tag="VGB16_01125"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_013248593.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_function: GO:0016887 - ATP hydrolysis activity
                     [Evidence IEA];
                     GO_function: GO:0042626 - ATPase-coupled transmembrane
                     transporter activity [Evidence IEA];
                     GO_function: GO:0140359 - ABC-type transporter activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="ABC transporter ATP-binding protein"
                     /protein_id="HEX9916312.1"
                     /translation="MPDEKKDTILECINLYKSFQTGAGRLDVLKGVDLEVKQGEILAV
                     IGASGVGKSTLLHILGALDKPTRGEVRYGGKSIFSFKDKELARFRNRKIGFVFQFHHL
                     LPEFSALENVMMPRLIAGEEKENIIDKALKLLSRVGIEDRATHRPAELSGGEQQRVAV
                     ARALISDPELVIADEPSGNLDQKNAQGLHNLLKELNQNQGYTFVIATHNQSLAQTAER
                     IITLKDGKAVLN"
     gene            complement(3768..4793)
                     /locus_tag="VGB16_01130"
     CDS             complement(3768..4793)
                     /locus_tag="VGB16_01130"
                     /inference="COORDINATES: protein motif:HMM:NF015983.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003824 - catalytic activity [Evidence
                     IEA];
                     GO_function: GO:0051539 - 4 iron, 4 sulfur cluster binding
                     [Evidence IEA];
                     GO_function: GO:1904047 - S-adenosyl-L-methionine binding
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="radical SAM/SPASM domain-containing protein"
                     /protein_id="HEX9916313.1"
                     /translation="MGLRFYQRFFLAYWAYSYREIELNYPPLFFSIEPTNRCNFKCIY
                     CPQSYSTGKNMEKGAMDMNLFEIIMQKIAQTKPINQIFLTGNGEPLLHSELEEFIRLT
                     CKHHLRPSFTSNGSLLHPDRINSLATSGSFSITIDFSPYKELYEKNRIGGNWEGVYAN
                     LKNLLKFKKEKNRQDIRVTIKDMSTILLENEKIKKDSLADLQKLFSGLPVDSFVSVVF
                     HNWIGNIDPKIVPASGLKPDYNPCSHPWSLMQINFKGEVVACCRDMAGEYIIGKIDNG
                     IEIMQLWNNKKMIELRKALVEKRTSDIRTCRYCDRPYRGGSIGKNKWEIIKTVLTKRK
                     KFLQAEI"
     gene            complement(4799..6025)
                     /locus_tag="VGB16_01135"
     CDS             complement(4799..6025)
                     /locus_tag="VGB16_01135"
                     /inference="COORDINATES: protein motif:HMM:TIGR02212.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0005886 - plasma membrane [Evidence IEA];
                     GO_process: GO:0042953 - lipoprotein transport [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="lipoprotein-releasing ABC transporter permease
                     subunit"
                     /protein_id="HEX9916314.1"
                     /translation="MPYELFIAKRYLKSKKRSGFLSVFTLISIGGVLIGVAALVFVLS
                     MMNGFEKEVRSRIIGNTAHINVYPIEREGIEDYTELISKIEKVKHVVAATPVITYKAA
                     IASKSSGDGIVVRGIDVARETKVTTIGQNMLDGNLNFQTQTDYPGIVLGAGLANRLGV
                     SIGDHVILFSLKEESISSGWVVPKASKFIVTGIFETGLYEYDISLAYVSLESAQRLFN
                     LENRVTNIQVKVDNLYKAGKIAREVENAVGYRFYAQDWMNMNKSLFSWMALEKYAMFI
                     VLSLIVAVAAFNIVSTLIMTVLDKKKDIGVLKSMGATQNSIMKIFMFKGLIVGIIGTV
                     SGSALGYLLCWVQKTFKVVSLPAEIYFINSLPIDMRVSDFALVVVAAIGITFLATIYP
                     ARQAAGLDPVEAIRYE"
     gene            complement(6142..>6874)
                     /locus_tag="VGB16_01140"
     CDS             complement(6142..>6874)
                     /locus_tag="VGB16_01140"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_013275006.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0000166 - nucleotide binding [Evidence
                     IEA];
                     GO_function: GO:0004812 - aminoacyl-tRNA ligase activity
                     [Evidence IEA];
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_process: GO:0043039 - tRNA aminoacylation [Evidence
                     IEA]"
                     /codon_start=2
                     /transl_table=11
                     /product="amino acid--tRNA ligase-related protein"
                     /protein_id="HEX9916315.1"
                     /translation="DFRNEGMDKSHNPEFSMLELYQAYVDYNDVMDLCQDMISQVAQD
                     VLGTKKITYQGNEIDLTPPWKRVSYLDSFKEYAGIDIQGKDENQIRQIVKELNLDTNL
                     EQNREKLLDVIFGSQVQNKLFSPTFVVDYPIEISPLAKHHREKKGLTERFEIFIGTCE
                     IGNAFSELNDPLDQRQRFEQQTKLAKAGDEEAFVLDEDFIRALEYGMPPTGGLGIGID
                     RLVMILTDSPSIRDVILFPQMRPER"
BASE COUNT         1780 a         1681 c         1263 g         2150 t
ORIGIN      
        1 caactcttct tttttgaatt tcagctcatc cctcaactgg gcggcttttt cgaattcctg
       61 attttttact gcctgctctt tttgcttctg tagatgtatt atctcttcct ctaattttcc
      121 gaagacatcc ggcttggtgc aggtggcaag gtgagctttt gaaccagcct catcgatgac
      181 atcgatggct ttatctggtt gaaactttcc agagatgtat cggtctgaaa ggttcacggc
      241 ggcttctaca gcttcatccg atatttttat cttatgatgc tcttcgtatc tgcttttcaa
      301 tccttgcagt atcttgacgg tatctcctaa agttggcggg tcaatcatca cggtttgaaa
      361 gcgtctctcc agagcgccat ccttttcaat atattttcga tagtcgttca aagtcgtggc
      421 accgatacat tgcatatccc ctcgggaaag agagggtttg aagatattcg aagcatccag
      481 ggagccctca gctccacctg cccccacgat ggtatgcagc tcgtcgatga agattatcac
      541 atctttagaa ttggccacct cagtcatcac cgctttcatt ctctcttcaa attggccacg
      601 gtatttggtt ccggctacca aggaagccat gtccagacta acaatcctct tgttctctag
      661 tatctgcgga accttattct caacaatctt ttcagccaaa ccttcaacaa tagcggtctt
      721 ccctactccc ggctcgccga tcaaaaccgg attgttcttc ttgcgccgtg agagaatctg
      781 agataatctc tcgatctcat tctcccgtcc aatgattggg tccagctttc ccttgcgagc
      841 caattcagtt aaatctctgc caaaatggtc caatgcggga gtcttgcttt tcttcctctt
      901 cttgccgtag gcagaaggtt tgccgctttg aatattctta agctcttcat atacttcctt
      961 gtaatctata tcatacatgg aaagaacttg ggctgccact ccctcctcat cccgcaacaa
     1021 agccagaagg agatgctccg tgtcaatctc attggacctt aaagcgcggg cttcctgtcc
     1081 ggatatctct aaggtctttt tggcgcgggc agttagggga agttgcccca tggtcatagt
     1141 tccaccggag ggctgaacca catcctcaat cgactgtctc agttcgttta aatctattcc
     1201 aatattcgcc ataatctcta tggctttccc ttctcccagg cgcaccaatc ccaaaaggag
     1261 gtgctccgtc cctatgtagt catgcctcag acgggcagct tcatctcgag catattctat
     1321 tgcttttctc gctttctcag taaacatctc gttcatacaa acacctttta tcctttatct
     1381 ataatctaat ttctctaacc acataagtca attgtaattt gccaacttgc ttctcaccag
     1441 ctctgctcgc atgatatccc tttcatcctc attcatatcc ttgttaaaat atttttgcag
     1501 atgagctggt tgagatagta tcaagatttc gttaatcagc ggtaagggta acaggtcaat
     1561 cgttcccata cctaatccca atctgatggc ggaaagtaaa ttcatcacct cttcagaggt
     1621 caagacccga gcgctcttca gtatcccata agctcgccaa atcttatcct cgatctcctc
     1681 tttggcatcc tggaataagg tttgtcgggc attagtctca tattcgatta tctgcttggt
     1741 tattttttcc agactttcaa taatatcctc ctccgaccga cccaaggagg tttgatttga
     1801 aatctgaaac agatttccca aaacatccgt gccctcacca tagaatcctc gaacggtcaa
     1861 tcccacttta gttatctggg aaattacttt ttccatctcc cgaatcaaga ccagggctgg
     1921 caggtgaatt aaaatcgaag ctcgcatccc tgttcccaca ttggttgggc aggaagtcag
     1981 atagccgaag tccgcatcaa aatcgaattc cagggattgt cccagatcat catctatctt
     2041 gtcggcgatg caacaggtat ctcttatttc cagcccggat tggattatct gaattcttaa
     2101 atggtcctcc tcgtttatca tgatgctcac catctcctca tcgctgacga aaagtgcgcg
     2161 cgaatcattc cctcgcacaa attccgggct tatcagatgt ctttccatta agagtctctt
     2221 gtcaagctca ctaagctcat tccataaaag gaaagtcccc tttttcaagt tagggctctt
     2281 atctatggct aatttggcaa aggtgatgac cctctcgcga ttctgtgcat ctgccaccga
     2341 ggtgaaaaga cagccggata tgtttcgtgc caaccggatg cgactggaaa gcactatccc
     2401 ggattgggtg ccatcaccgg ctaaccaaga agctggcttt ttgacaatct cctcaaacat
     2461 tcttatttac cttcaacatt tttttcttta atcaatgttt tgattctgtc tcttatccga
     2521 gcagcttcct caaaatcctc ttgctctacc gctcttttca gttcctcctg taattttctt
     2581 tcctcgcgca aaggtttcat cgaatctccg ggaaaataag gtatttttcc gcgatgtcgg
     2641 tttgagccat gtatctttct taaaaggtcg ttgagttgaa cgcggaaagt ggtataacag
     2701 cttccacatc ctaatcttcc tacccgacta aactcagcaa aagtcatctg acagttggga
     2761 catttgacct cagctaatgc tatcccctcc tttttaactc ctttatccag catgctggac
     2821 aagaattcag ccagagggaa ggggacctct tcaaagggac tatggaatcc ccttttctcc
     2881 gcacaatcct tgcacaagtt cagcatcatt ttctcgtcgt ttatcacctg cgtccactga
     2941 attgtggctt tgttcttctt gcaatcctca cacaacattt cttttccttt agtttagaac
     3001 tgccttacca tcctttagag tgattatcct ttcagctgtt tgtgccagac tctggttatg
     3061 cgtggcaatc acaaatgtgt atccctggtt ttgatttaat tccttcagta aattatgcaa
     3121 gccctgcgca ttcttctggt ctaaatttcc tgatggctca tccgcaatta ctagttcggg
     3181 atcgctgata agcgccctgg caactgcgac tcgctgttgc tcccctccgg aaagttcagc
     3241 tggtcggtga gttgccctat cctcgattcc cactcgagac aagagcttca aagccttatc
     3301 aattatattc tctttttcct ctcctgctat cagtcgaggc atcatcacgt tctctaaagc
     3361 actgaactct ggcaacaagt gatgaaattg gaatacaaaa ccgatctttc gattgcgaaa
     3421 ccgggctaac tccttgtctt taaaggagaa tatactcttt cccccgtatc ttacctcccc
     3481 ccgagttggt ttatccaaag cccccaatat atgcaagagg gtacttttgc caacaccaga
     3541 ggcgccgatt acggctaata tctcaccctg ttttacctcc aggtctacac cttttaatac
     3601 gtcaagtctg cctgcgcctg tttgaaagct tttatacaaa tttatgcatt ccaagatagt
     3661 atcctttttt tcgtccggca ttgactatat tcctgctata tctattttcg ccacttttct
     3721 taatatattt aacaatatcc caacttaatt gtcaactcct ttaattgtca aatctcagct
     3781 tggaggaatt tctttctttt agttagaacg gttttgatga tctcccattt gttttttccg
     3841 atgcttccac ctctgtacgg tcgatcacaa tatctacaag ttcttatatc agatgttctt
     3901 ttttcgacca aagcctttct taattctatc attttcttat tgttccagag ttgcattatt
     3961 tcaattccat tatctatttt accaataata tattctcctg ccatatcccg acagcaagct
     4021 actacctcac ccttgaagtt tatctgcata agcgaccaag gatgagaaca tggattgtag
     4081 tctggtttaa ggccggaagc aggaacaatt ttcgggtcga tatttccaat ccaattatgg
     4141 aaaacaacgg agacgaaact atcaactgga agacccgaaa ataacttctg caggtctgca
     4201 agcgaatcct tcttaatttt ctcattttct aatagaatgg ttgacatatc cttgatagtc
     4261 actcttatat cctggcggtt tttttctttt ttaaatttca aaagattttt taggttagca
     4321 taaactccct cccagttgcc gccgattcta tttttctcat acaattcctt ataaggcgag
     4381 aaatcaattg tgatagaaaa acttcccgaa gttgctaagg aatttatgcg gtcaggatgg
     4441 agcagggagc catttgaggt gaaactcgga cgaagatggt gcttacaggt cagtctgatg
     4501 aactcctcca attctgaatg gagcagtggc tcgccattac cagtaaggaa aatctgattt
     4561 atcggtttgg tctgggcaat tttttgcatg ataatctcga ataaattcat atccatagcc
     4621 cccttttcca tattcttgcc ggtggaataa ctttgagggc aatagataca tttgaaatta
     4681 cagcgattgg tcggctcgat ggaaaagaag agcggaggat agttcagttc gatttctcta
     4741 taagaataag cccaataagc taaaaagaac ctttgataga atcttaagcc catcttgatt
     4801 attcatacct tatggcttcg accgggtcaa gtccggcagc ttgtcgggca ggataaattg
     4861 tggctaaaaa agttatgcct atggcggcaa cgaccaccag cgcaaagtcg gagacgcgca
     4921 tatctatggg aagggaattt ataaagtata tctcagctgg cagggagacg accttgaagg
     4981 tcttttgcac ccagcacaaa agataaccta aggcacttcc tgacaccgtc ccaattattc
     5041 cgacgatcaa ccccttgaac ataaaaatct tcattatact attttgagtt gctcccatag
     5101 atttcaagac tccgatatcc tttttcttat ctagaacggt cattatcaga gtgctcacga
     5161 tattgaaagc tgccaccgcc acaattagac tcaaaacgat gaacatcgca tatttctcca
     5221 aagccatcca agagaaaaga ctcttgttca tattcatcca gtcctgagca tagaagcggt
     5281 agcccaccgc gttctcaact tccctggcga tctttcctgc tttatacaga ttgtccacct
     5341 tcacctggat gttagttacc cgattttcta aattgaataa cctctgagca gactcaagcg
     5401 aaacataagc taaggagata tcatattcat ataacccggt ctcaaagatt ccagttacta
     5461 taaacttgct ggctttggga acaacccagc cggaggagat ggattcctcc tttaaactga
     5521 aaagaatcac atggtctcct atgctaaccc ctaagcgatt agccagacca gcacccaaca
     5581 ctattcccgg atagtcagtt tgagtttgga aattcaaatt tccatcaagc atattctggc
     5641 ctatggtggt aactttggtt tctctggcta cgtcaattcc cctaactaca atcccatccc
     5701 cgctactctt agaagcaata gcagctttat aagtgattac cggggtagcg gcaaccacat
     5761 gttttacttt ttctattttt gaaatcaatt ccgtataatc ctcaattcct tccctttcga
     5821 tgggatagac atttatgtgg gcagtatttc cgattattct agagcgcacc tctttttcaa
     5881 atccgttcat catagataaa acaaagacca gggcagctac cccaatcaag acaccgccaa
     5941 ttgagattag ggtgaaaacc gaaagaaatc cagacctctt cttggatttc aaatatcttt
     6001 tggcaatgaa taattcgtag ggcataaaaa attaaaaatt aaaaatgaaa aattgaaaat
     6061 taaaattcaa aactatatct ttcttttacc ttttggaact ttgaattgtg attttgcatt
     6121 ttgctttttg gattttgaat tctatcgttc tggtctcatc tggggaaaga ggatgacatc
     6181 tcgaatagaa ggggaatcgg tcaagatcat cactaatcta tcgatgccta tgcccaagcc
     6241 accagtggga ggcattccat attcgagggc acggatgaaa tcctcatcta agacaaaagc
     6301 ctcctcgtct ccggctttag ccaacttggt ttgctgttcg aatctctgtc tctggtcaag
     6361 agggtcattc agctcggaaa aagcatttcc tatttcacag gtgccgatga aaatctcaaa
     6421 acgctcggtc aaacctttct tttcccgatg atgtttggct aagggagaaa tctcgatagg
     6481 gtaatccacg acaaacgtgg gggagaacag tttgttttgc acttgacttc caaatatgac
     6541 atcaaggagt ttttccctat tctgctctaa gttcgtatcc agatttaatt ccttaactat
     6601 ttgcctgatt tggttttcat cttttccctg gatatcgatg ccagcatatt ctttaaacga
     6661 atccagataa gaaacccttt tccagggagg ggttaggtca atttcatttc cctgataagt
     6721 tattttcttg gttcccagaa catcctgagc aacctgggat atcatatcct gacataaatc
     6781 cattacatcg ttatagtcaa cgtaagcctg atacagctcc agcattgaaa attcaggatt
     6841 gtgagatttg tccataccct catttctgaa atct
//
LOCUS       DASWLN010000032         2237 bp    DNA     linear   ENV 20-FEB-2024
DEFINITION  MAG TPA_asm: Candidatus Zixiibacteriota bacterium isolate
            SMAG_U2949 k141_667031, whole genome shotgun sequence.
ACCESSION   DASWLN010000032 DASWLN010000000
VERSION     DASWLN010000032.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37960594
            Sequence Read Archive: SRR10376091
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Candidatus Zixiibacteriota bacterium (soil metagenome)
  ORGANISM  Candidatus Zixiibacteriota bacterium
            Bacteria.
REFERENCE   1  (bases 1 to 2237)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 2237)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (26-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/12/2024 08:28:26
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 1,440
            CDSs (total)                      :: 1,416
            Genes (coding)                    :: 1,412
            CDSs (with protein)               :: 1,412
            Genes (RNA)                       :: 24
            rRNAs                             :: 1 (16S)
            partial rRNAs                     :: 1 (16S)
            tRNAs                             :: 21
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 4
            CDSs (without protein)            :: 4
            Pseudo Genes (ambiguous residues) :: 0 of 4
            Pseudo Genes (frameshifted)       :: 0 of 4
            Pseudo Genes (incomplete)         :: 3 of 4
            Pseudo Genes (internal stop)      :: 1 of 4
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2237
                     /organism="Candidatus Zixiibacteriota bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_667031"
                     /isolate="SMAG_U2949"
                     /isolation_source="soil"
                     /db_xref="taxon:2053527"
                     /environmental_sample
                     /country="USA: Oregon"
                     /lat_lon="42.99 N 123.63 W"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            <1..405
                     /locus_tag="VGB16_01145"
     CDS             <1..405
                     /locus_tag="VGB16_01145"
                     /inference="COORDINATES: protein motif:HMM:NF014077.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016787 - hydrolase activity [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="5'/3'-nucleotidase SurE"
                     /protein_id="HEX9916316.1"
                     /translation="EGTILRIPSIAVSMCNWEKGSFKAGARFVLMLSKLILKKGLPKD
                     TFLNVNFPSRKNLKDYRITELGRVSYRDVVIQQIDPRGQRYYWIGEQFPFTKAQDQSD
                     FDAIKKGLVSITPLHLDMTNYVCMKELKKWKI"
     gene            595..1693
                     /gene="prfB"
                     /locus_tag="VGB16_01150"
     CDS             join(595..660,662..1693)
                     /gene="prfB"
                     /locus_tag="VGB16_01150"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_019541934.1"
                     /ribosomal_slippage
                     /note="programmed frameshift; Derived by automated
                     computational analysis using gene prediction method:
                     Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="peptide chain release factor 2"
                     /protein_id="HEX9916317.1"
                     /translation="MPETNFNLTEYKSKLEKLREYLEIEKKEEQIGELEKTSQKSDFW
                     EDGEKAKAVLKQIGSLKRIVDKWRDLSKNLEDVKVLYELAQEEGDVSVQKEALDNFEK
                     LKKEIDDFEFTTLLSEEDDSKNVLLSIHSGAGGTESQDWAQMLLRMYMRWIERKGFGH
                     QVIDLQSGEEAGIKSATLEVTGDYAYGFLKGESGVHRLVRISPFDANKRRHTSFASVF
                     VYPEIEDNITVEIRDDDIKIDTFRASGAGGQHVNKTSSAVRITHIPTGIVVQCQSERS
                     QFQNKDNALKVLKAKLYQHYKEKEEEKQAKLESKKKKIEWGSQIRSYVFHPYNMVKDH
                     RTDEQTSNAQGVMDGDIDKFIQAYLNRKEKD"
     gene            1767..>2237
                     /locus_tag="VGB16_01155"
     CDS             1767..>2237
                     /locus_tag="VGB16_01155"
                     /inference="COORDINATES: protein motif:HMM:NF013665.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016746 - acyltransferase activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="phosphate acyltransferase"
                     /protein_id="HEX9916318.1"
                     /translation="MSRIWEKAKNKKKKVVLPEGTEPRMILATKKILSEGLAGVTLLG
                     DENQIRKLAQENGLDLSKVEVINPAKSDKLDEYAEEYFKLREKKGMTKDKAKVWMQNP
                     LFFGAMMTRFGKVDASVAGSINTTGDVLRAGIHIIGLAPGIKTVSSSFIMVIPEF"
BASE COUNT          738 a          409 c          556 g          534 t
ORIGIN      
        1 gagggaacca tcttgagaat accctcaatc gcggtctcaa tgtgcaattg ggaaaagggg
       61 agtttcaaag caggtgcgag gtttgtcctt atgctctcca agcttatcct gaaaaaagga
      121 cttcctaaag atacattcct gaatgttaat tttccttcca gaaaaaatct aaaagattac
      181 cgaataaccg agttaggtcg tgtttcgtat cgggatgtcg tcattcagca aatagatcct
      241 cgtggtcaga gatattattg gatcggtgag cagtttccct tcaccaaagc acaagaccag
      301 agtgatttcg atgccatcaa gaaagggctt gtctccatca cccccctgca tttggatatg
      361 accaattacg tttgcatgaa agagctcaag aaatggaaga tatgacctcc tctccacaaa
      421 cggattgact tttaaggcaa tcggattata tttaattaca aaatcaatcc ttcgacacca
      481 cgacctgaag gccgtggatt gctttaggat tgatactgag agcggtgtat tctttcgccg
      541 ccctaaaggg cgggttttga gcgccgccga acgtatcaaa aaaggtgaaa aattatgccg
      601 gaaacaaatt tcaatttaac ggaatataaa agcaagttgg aaaaattacg ggagtatctt
      661 tgagatagaa aagaaggaag agcaaatagg tgaactggag aaaaccagcc agaaatccga
      721 cttctgggaa gatggagaaa aagccaaagc cgttctcaag cagatagggt ctctcaaaag
      781 aatagtggat aagtggagag atctgagcaa gaacttagaa gatgtcaagg tcttgtatga
      841 gttggcacaa gaggaagggg atgtctcggt tcagaaagag gcactggata atttcgaaaa
      901 actgaaaaaa gaaattgatg attttgaatt caccaccttg ctttcggagg aggacgattc
      961 caagaatgtt cttctttcca ttcattctgg ggcaggtggg actgaatctc aggattgggc
     1021 ccagatgctt ctgaggatgt atatgcgctg gatagaaaga aagggatttg gtcaccaggt
     1081 aatagacctg caatccggtg aagaagcagg cataaagagc gccaccttgg aggtaaccgg
     1141 ggattatgct tatggatttt taaaagggga gagtggggtt catcgcctgg tgcggatttc
     1201 cccttttgat gccaacaaga gaaggcatac ttcgtttgcc tcggtatttg tctatccgga
     1261 gatagaagac aatatcaccg tggaaataag agatgacgat ataaagatag atacctttcg
     1321 ggcttcgggc gcaggtggcc agcatgtcaa caaaaccagt tcagctgtac gcattaccca
     1381 catccccacc ggaatcgtgg ttcagtgcca gtccgagagg tcgcagtttc aaaataagga
     1441 caacgcatta aaagtgctta aagcgaagct ttatcaacac tacaaggaga aagaggagga
     1501 gaaacaggca aaactggaaa gcaagaaaaa gaagatagaa tgggggagcc agataaggtc
     1561 gtatgttttc catccttaca atatggtcaa agaccatcgc acggacgaac agacctcaaa
     1621 tgctcaaggg gtgatggatg gggacatcga caaattcata caggcttatt tgaataggaa
     1681 agaaaaagat taaacagagc atacagtttc ttatactcag cagaatacag aatcgaaata
     1741 tttagaaagg atttcttatg tccgtgatgt caagaatttg ggaaaaggca aaaaataaga
     1801 aaaagaaggt ggtcctgcca gaaggaactg agccgagaat gattttagcc accaagaaaa
     1861 ttctctccga aggattagct ggggtcacgc ttctgggaga tgaaaatcag attagaaaat
     1921 tggctcagga aaatggactg gacctttcaa aagtagaagt aattaatcct gctaaatccg
     1981 ataagctgga tgaatatgca gaggaatatt tcaagctccg cgagaaaaaa ggaatgacca
     2041 aagataaggc aaaagtatgg atgcaaaacc ctcttttctt cggagcaatg atgactagat
     2101 ttggtaaggt ggatgctagc gtggcagggt caattaatac caccggagac gtcttgagag
     2161 ctggaattca tattatcggg ttggctcctg ggattaagac tgtttccagc tcattcataa
     2221 tggtaatccc cgaattt
//
LOCUS       DASWLN010000033         8900 bp    DNA     linear   ENV 20-FEB-2024
DEFINITION  MAG TPA_asm: Candidatus Zixiibacteriota bacterium isolate
            SMAG_U2949 k141_285993, whole genome shotgun sequence.
ACCESSION   DASWLN010000033 DASWLN010000000
VERSION     DASWLN010000033.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37960594
            Sequence Read Archive: SRR10376091
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Candidatus Zixiibacteriota bacterium (soil metagenome)
  ORGANISM  Candidatus Zixiibacteriota bacterium
            Bacteria.
REFERENCE   1  (bases 1 to 8900)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 8900)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (26-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/12/2024 08:28:26
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 1,440
            CDSs (total)                      :: 1,416
            Genes (coding)                    :: 1,412
            CDSs (with protein)               :: 1,412
            Genes (RNA)                       :: 24
            rRNAs                             :: 1 (16S)
            partial rRNAs                     :: 1 (16S)
            tRNAs                             :: 21
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 4
            CDSs (without protein)            :: 4
            Pseudo Genes (ambiguous residues) :: 0 of 4
            Pseudo Genes (frameshifted)       :: 0 of 4
            Pseudo Genes (incomplete)         :: 3 of 4
            Pseudo Genes (internal stop)      :: 1 of 4
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..8900
                     /organism="Candidatus Zixiibacteriota bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_285993"
                     /isolate="SMAG_U2949"
                     /isolation_source="soil"
                     /db_xref="taxon:2053527"
                     /environmental_sample
                     /country="USA: Oregon"
                     /lat_lon="42.99 N 123.63 W"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            <1..1133
                     /locus_tag="VGB16_01160"
     CDS             <1..1133
                     /locus_tag="VGB16_01160"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=3
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEX9916319.1"
                     /translation="FGFSDSVIWIATNQGLKRSLDWGNSWDSIEVKDASADSSYFKTP
                     LAHRLKSYDLNTVLVSDSIIWVGGKDGLARSTYPDSVWTIYNFFVSYEQAVWTGSAAG
                     IFKFIYNHSDSADIALQYTISDNLTGNFVVALTIQKYGSKRVIWAATNPTFSGTYGIS
                     KSDNEGKTWTKCLEGTRVWNFAFQDSIVWAATDSGLERSFDYGENWEVFNYMKDSNDT
                     LTQNRIFNTTFYSVCLVKDTSNGKWTVWAGNVDGLVRSVDLDNNKWQVFRSFVPIGTP
                     GSETAYAYPSPFSPMISSGGRIRIHHRPLADSYVTIKVYDFAMNLVTTIVDNQFRYGG
                     RDYDEPWDGRNDRGALVANGVYFFRMETSLGQKEWGKLVILK"
     gene            1142..2083
                     /locus_tag="VGB16_01165"
     CDS             1142..2083
                     /locus_tag="VGB16_01165"
                     /inference="COORDINATES: protein motif:HMM:NF033709.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="PorV/PorQ family protein"
                     /protein_id="HEX9916320.1"
                     /translation="MKKRIVIILMLSIFSILSASYAEEGNGGYAGPFRQLGLGARAMG
                     MGGAFVSIADDATACFWNPAGLVQLKERKVGACYRMMFLDRRMSYVSYAQPAKEEAGI
                     GLAWVNAGVGNVMGRDSNGEPTEKISNSINSFSLSFARRFSSWFGLGMNLRYTQFNLA
                     GINSYGLGFDFGVHVESKMGIRAGAVVEDVGMKYSWSSGKYWGKYGMTGSSINERFPV
                     KVRLGASYLAFKEKLLVAFDIHKSEYQNVEFSSGGEYWLIKNLAGRIGYDRGVFTFGL
                     GFKHYLDEEKNISIDYVFLTSQVEGDPDHMISMQFGF"
     gene            2299..3213
                     /locus_tag="VGB16_01170"
     CDS             2299..3213
                     /locus_tag="VGB16_01170"
                     /inference="COORDINATES: protein motif:HMM:NF033709.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="PorV/PorQ family protein"
                     /protein_id="HEX9916321.1"
                     /translation="MRKALALSFLSIFLAGLCYGGDISERAGTYGLSFLKVGMGARPV
                     GMGEAFTAISGDINSIYWNPAGLARIEGIDLTFMHNRWFQEISSNYLATAFKIRKNSI
                     GISLALNRVPDIEVRDRPTAEPIGTFDAEDLVLTLGCARSLGAKFDLGISVKGLYQKI
                     YTYEASGLGFDIGGVYVLNDKFQFGTAILNLGPKMKFEEEKFSLPLICKLGAAYKTSE
                     KQLNGDIILGLDLIKPRDSDLKLHSGIEYNYDEILSLRIGYQSGYDDKSFSFGMGLGY
                     ERYKVDYAFVPFSSDLGNTHRISLEIKM"
     gene            3222..4343
                     /locus_tag="VGB16_01175"
     CDS             3222..4343
                     /locus_tag="VGB16_01175"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_004558303.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="isocitrate/isopropylmalate dehydrogenase family
                     protein"
                     /protein_id="HEX9916322.1"
                     /translation="MPKYKIAWLPGDGIGKDVMQAARIVLDAIKLDAQYIPGDIGWEF
                     WCKGGDALPQRTIKLLKETDCCLFGAITSKPKEEAEAELCQNLKGKGVVYTSPIVRLR
                     QEFNLRTNLRPCKAYAGNPLNYRDDIDIVVFRENTEDLYSGVEFHPLPQEVMDVLCKH
                     NPKMTKFKQTPLEDIAVSLRIITRKASQNIVRDAFEYAKKYGRKSVTVIEKPNVIRET
                     SGLVIREARKIAKEYPEIKFNEANIDAICMWLLKNPQDYDVLATSNMFGDIISDLCAQ
                     LVGGLGFASSGNIGDDYAIFEPTHGSAPKYAGQFKVNPIATFLSAKLMLDWLGEKEKS
                     ERLEKAIAEVIKEGKVRTYDMGGKSTSLDVANAVAGKLK"
     gene            complement(4625..5695)
                     /locus_tag="VGB16_01180"
     CDS             complement(4625..5695)
                     /locus_tag="VGB16_01180"
                     /inference="COORDINATES: protein motif:HMM:NF013740.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0015562 - efflux transmembrane transporter
                     activity [Evidence IEA];
                     GO_process: GO:1905887 - autoinducer AI-2 transmembrane
                     transport [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="AI-2E family transporter"
                     /protein_id="HEX9916323.1"
                     /translation="MTNNTKSYLTLQNIALPFIALAAATAFFYFASPILIPVVLAASF
                     SYILSPAVKLLNRIKLPHMVSVFIVVLVAIFVVAVAGYFLFGIAQGLVGDFPNYWDAV
                     IKFLSQTRQDIKILDKILPSQESLDLKTLQLKDVSGLTGFVAKSAGSLFTFVFISILT
                     AFLTILLLSEQKGLKEKLIYAFGKNERSVAENIIAQINKQIGKFLVVRFLTSLGLGIV
                     FAIGLAIIGVKYAIFWGLLAGVMDLVPFIGPVLAVVPPLIVTGVQFKSVMPMFWVFIL
                     YEAIQIPESNLVAPRLMGRSLNLSPFALLVATMYWAWLWGAIGVILAIPITAAMKVIC
                     DHIEPLEPIGILLGGKVEKEVS"
     gene            complement(5696..7402)
                     /gene="polX"
                     /locus_tag="VGB16_01185"
     CDS             complement(5696..7402)
                     /gene="polX"
                     /locus_tag="VGB16_01185"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_015739757.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003677 - DNA binding [Evidence IEA];
                     GO_function: GO:0003887 - DNA-directed DNA polymerase
                     activity [Evidence IEA];
                     GO_process: GO:0006281 - DNA repair [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="DNA polymerase/3'-5' exonuclease PolX"
                     /protein_id="HEX9916324.1"
                     /translation="MNNREIAQIFYSIAEILDIQGENPFRIRAYIRAGQTIENLTEQI
                     SDMPDIDQLKELPGIGDAILEKIKEILKTGRLKFYEKLKKSEYAPLIELLKVPGMGPK
                     HAKLVYDQLHIRTTKQLEKAARAGRLRELPGLGPKVEQNILQGIKQAIKHKERMPLAF
                     VYPLAQAVLKEVEKMKQVKKAILAGSLRRMKETIADIDILASAEKSKPIIDAFVKIPQ
                     VKKVLAKGSTKSSIQIENNIQVDLRIVKPESFGSASHYFTGSKAHNIKLRSIAQARGL
                     KVSEYGIFKGKKRIAGRTEEEVYSALGLPYIPPEIREDNGEIEAGFENRLPKLIELKD
                     IKGDLHVHSNWSDGKRTIEDMVRYAKKIGYEYMAICDHSPYVGITNGLDEKRLERQMK
                     EIDKLNEKLEGIVVLKGIEVDIRGDGKLDLPDSILKKLDVVVASVHTKFSQSKEEMTK
                     RIIRAVENPNVDIIAHPTGRLIGKREPYEVGMDKLFDAVLANRKFLELNAYPQRLDLN
                     DINCRKAKEKGIKLVISTDSHTESDLEFMQFGVATARRGWLSKKDVLNTLHFNDFIRY
                     FR"
     gene            complement(7975..>8900)
                     /locus_tag="VGB16_01190"
     CDS             complement(7975..>8900)
                     /locus_tag="VGB16_01190"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_007653306.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008236 - serine-type peptidase activity
                     [Evidence IEA];
                     GO_process: GO:0006508 - proteolysis [Evidence IEA]"
                     /codon_start=3
                     /transl_table=11
                     /product="S41 family peptidase"
                     /protein_id="HEX9916325.1"
                     /translation="QFVAPYFSSSTSYVLQDLSVSNLLQGLKGDCFEITLKTPNGKLK
                     QFLLTHERTSEQEVYPPFEKEDTLYELLQFKWYDHQIAYLALNSFGDPKIDTLFEQHL
                     PDLYKAKALIIDLRNNGGGNEPIGKQILQYLTPDTLLYGYRCFSRMHIPAYKAWGADV
                     KPEDTTGNDWAKKCLLDFQDKAYYTFDYSPDTIHVLEKKVVVPTVLLIGHNTASAAED
                     FLIYADNQKHMVKIGENSYGSTGQPYIFELPGGGEAWVCTKKNTYPDGREFVGYGVKP
                     DIEVIPTVQDYLRHNDPVLSKALEFLGRKPK"
BASE COUNT         2414 a         1836 c         2094 g         2556 t
ORIGIN      
        1 atttcggatt ttccgattcg gtcatctgga tagcaaccaa tcagggactt aaaaggtctt
       61 tggattgggg gaacagttgg gatagtatag aggttaagga tgcttcagct gattcatctt
      121 attttaaaac gcctttagcc cataggttga aatcatacga cttgaatacg gttttagtct
      181 cggatagcat aatctgggta ggaggaaaag atgggctggc tcgttcaact tatcccgatt
      241 cggtgtggac catctacaac ttttttgttt cctacgagca agcagtctgg acaggtagcg
      301 ctgccggcat ctttaaattc atttacaacc acagcgatag cgcagatata gccctgcaat
      361 ataccatttc cgataattta actggaaatt ttgtggtggc tttgacaatc caaaaatatg
      421 gcagcaagag ggtaatctgg gctgccacca atccaacctt tagtggaact tacggaataa
      481 gtaaatcgga taatgaggga aaaacctgga ccaagtgttt ggagggaaca agagtgtgga
      541 attttgcttt tcaggactcg attgtctggg cggcaaccga ttcaggtttg gagaggtcct
      601 ttgattacgg agagaactgg gaagttttca attacatgaa agattcaaat gatactttga
      661 cccaaaatag aatattcaat actacctttt attctgtctg cttagtcaaa gatacatcca
      721 atggtaagtg gacagtgtgg gcagggaatg tggatggttt ggttaggtca gtagatttgg
      781 ataacaataa gtggcaagtc ttccgctcct ttgtgcccat tggaacgcca ggctccgaga
      841 ccgcttatgc ctatccttct ccgttttctc cgatgatatc atccggagga agaattcgca
      901 ttcatcacag accgttagcg gatagctacg ttaccataaa ggtatatgat tttgcaatga
      961 atctggttac caccatagtg gataatcaat ttcgctatgg cgggagagat tacgatgaac
     1021 cctgggacgg tagaaatgat agaggggctc tggtggcaaa tggagtttat tttttcagaa
     1081 tggagacaag ccttggtcag aaagagtggg gaaagttggt aatattgaaa taggagagaa
     1141 aatgaaaaag agaatcgtaa taattctgat gttatccatt ttttctatcc tgtctgcaag
     1201 ttacgcagaa gaggggaatg gcggatacgc aggaccattt agacaattgg gcttgggagc
     1261 aagggctatg ggaatgggtg gcgctttcgt ttcaattgcg gatgatgcta ccgcttgttt
     1321 ttggaatcca gccgggctgg ttcagttaaa ggaaagaaaa gtgggcgcct gctatcgaat
     1381 gatgttcttg gatagaagga tgtcttatgt cagctatgct cagccagcca aagaagaggc
     1441 aggtatagga ttggcctggg tcaatgccgg ggtggggaat gttatgggaa gggatagcaa
     1501 cggtgagcca actgagaaga tatcaaactc aattaattct tttagcttga gttttgcccg
     1561 caggttctct tcttggtttg ggttgggaat gaatctcaga tatacccagt ttaacctggc
     1621 aggtatcaat tcttatggct taggatttga tttcggggtt catgttgaat ccaaaatggg
     1681 tatcagggcg ggagcagtgg ttgaggatgt gggaatgaaa tattcttggt cttcagggaa
     1741 atactgggga aaatacggaa tgaccggttc atccataaat gagaggtttc ccgtaaaggt
     1801 gcgtttggga gcctcctatc ttgccttcaa ggagaaactg ctagttgcct tcgatattca
     1861 taaaagcgaa tatcaaaatg tcgaattcag cagtggaggg gagtattggt tgataaagaa
     1921 tcttgcaggc aggattggat acgatagagg ggtcttcacc tttggcttag ggttcaagca
     1981 ctatttggat gaagaaaaga atataagtat cgattacgtt tttcttacca gccaggttga
     2041 gggagaccct gaccacatga tatcgatgca gtttggattc taatgcaaac acatttattc
     2101 gagcttggtc aaatctcccc tagccctcgc ctcgattcgt cccgcctcgg cgagccatcg
     2161 gcgagacggg cgaagcgggc ctcttttcta aagaggggaa aaatcccttc cccctttgaa
     2221 aaagggggaa caagggggat ttgaaaaata attgcatatt gcctaccatg taacagaact
     2281 taatgcgttt gctataatat gagaaaagcg cttgctctat cttttttgtc catttttttg
     2341 gctggcttat gctatggcgg agacatttcc gagagggccg gcacttacgg tctgagcttt
     2401 ctgaaagttg gtatgggagc aaggccggtt gggatgggtg aggctttcac tgccatttca
     2461 ggcgatatca attcgatcta ctggaatcca gctggcttgg caagaataga gggaatagac
     2521 ctcaccttca tgcacaaccg ctggtttcag gagataagct ccaactactt agcaactgcc
     2581 ttcaaaataa ggaaaaacag tataggaatt agtttagctt tgaatcgcgt cccggatatc
     2641 gaagtgaggg atagaccaac cgcagagccg attggcactt ttgatgcaga ggatttggtt
     2701 ttgaccttag gttgcgccag aagtcttggt gcgaagtttg atttgggaat ttcagttaag
     2761 gggttatatc aaaagatata tacttatgaa gcttcaggtt tgggatttga tattggcggc
     2821 gtatatgtct taaatgataa attccaattc ggaaccgcga ttttaaatct tggtcccaaa
     2881 atgaagtttg aagaggagaa attctcctta ccactaatat gtaaattagg agcagcttac
     2941 aagacaagtg aaaagcaatt gaatggggat atcatcttgg gtttggatct gatcaagcca
     3001 agagatagtg atctaaagct tcattccggg attgagtata attacgatga aattctatct
     3061 ttgagaatcg gttatcaatc gggttatgat gataaaagtt tcagctttgg aatgggcttg
     3121 ggatacgaac gatacaaagt ggattacgct tttgttccgt tcagctctga tttgggaaac
     3181 acacatagga tttctttaga gataaagatg tgaggaggat tatgcctaaa tacaaaattg
     3241 cctggttgcc cggagacgga attggaaaag atgtgatgca agccgctcgc attgttctgg
     3301 atgcaatcaa gttggatgcc cagtacatcc ccggagatat cggatgggag ttctggtgta
     3361 aggggggaga cgctctgcca caaaggacga ttaagctttt aaaagagact gattgctgct
     3421 tgttcggtgc cataacttca aagccaaagg aagaggcaga agcagaactt tgtcaaaatc
     3481 tgaagggaaa aggcgttgtc tataccagcc caatagttcg gcttcgtcag gaattcaatt
     3541 tgcggactaa cttgaggccg tgtaaagcat atgctggaaa tcccttgaat taccgagacg
     3601 acatagacat cgtagttttt agagagaaca ctgaagacct atattccgga gtcgaatttc
     3661 atcctttgcc tcaagaagtg atggatgttc tctgtaagca taatcccaaa atgacaaaat
     3721 tcaaacagac ccccttggag gacatagctg ttagcttgag aattatcacc cgaaaagcat
     3781 ctcagaatat tgtgcgagat gctttcgaat atgccaaaaa atatggacgt aaatcggtaa
     3841 ctgtaatcga gaagccaaac gtgataaggg agacctcggg cctggtgata agggaagcca
     3901 gaaaaatcgc caaagaatat cctgaaatca aattcaatga ggcgaacatc gatgctatat
     3961 gtatgtggct tttaaaaaat ccgcaggatt acgatgtttt ggcgacctca aatatgttcg
     4021 gagatatcat ctccgacttg tgtgcccagt tggttggagg tttaggtttc gcctccagcg
     4081 gcaatatcgg tgatgattac gctatctttg agccaaccca tggctcagcc ccgaaatatg
     4141 ccggacagtt caaagtcaat ccaattgcca catttttgtc agctaagttg atgctggatt
     4201 ggttagggga gaaggagaaa tcggaaagat tagaaaaagc cattgccgaa gtgataaaag
     4261 agggtaaagt tagaacctac gatatgggcg gaaaatcgac cagtttggat gtggcgaatg
     4321 cagtagcagg gaagttaaag taatggttta agataagctt ataagttgtt gtaatgtaaa
     4381 gtattaatgt aggaatagaa tagtgttctg ttcctacatt cttgcttcac gctgtcgttg
     4441 tgagtcctga tttatcgaga cggagcaatc tatataggta ttcccacctt gccctcaagg
     4501 tgggaattgg taaacccacg ttgggggcaa cgtgggttta ccgtttttcg tgggggcggg
     4561 gttcataccc gccctgcttt tttttgtaat tgtaggggcg acccgagggg tcgcccctac
     4621 agaattatga cacttccttc tcaacttttc cacccaaaag aatccctatc ggctctaagg
     4681 gctcgatatg gtcgcagatg actttcattg cggcggtgat ggggatagcc agtatcactc
     4741 caattgctcc ccagagccaa gcccaataca ttgtagccac cagaagggca aaagggctca
     4801 aattcaaact ccgtcccata agtcgaggag caacgaggtt actctccgga atctgtatag
     4861 cttcatatag aatgaaaacc cagaacatcg gcatcaccga tttgaattga accccggtca
     4921 caatcaacgg cggaacaaca gctaaaacag gaccaataaa gggaacgagg tccatcaccc
     4981 cagccagaag cccccaaaaa atggcatatt ttactccaat tatagccaag ccgatagcga
     5041 aaactattcc caggcccaag ctggtcaaaa acctcaccac taaaaattta cctatttgtt
     5101 tgttaatctg ggctatgatg ttctccgcca cacttctttc gttttttcca aaggcataaa
     5161 tcaatttttc ttttagtccc ttttgctcgg agagaagcaa tattgtcaag aaggccgtca
     5221 ggatgctaat gaagacaaaa gtgaaaaggg agccggcgct tttggctaca aagccggtca
     5281 aaccggagac gtcttttagt tgtaaagttt ttaggtcgag cgactcctgc gagggaagga
     5341 ttttatctaa tatctttata tcctgtctgg tttgactcaa gaattttatt accgcatccc
     5401 aatagtttgg aaaatcgcct accagtcctt gagcaatccc gaatagaaaa tatccagcca
     5461 cggcaactac aaaaatagct actagcacca caatgaaaac cgataccata tgcggtagct
     5521 tgattcgatt gagtaacttg acagccgggc taagtatata ggaaaatgat gcggccaaca
     5581 caaccggaat gagaattgga ctggcaaaat agaaaaaggc agtagcggct gccagggcaa
     5641 tgaaaggcaa agcgatattc tgtaaggtta agtaactttt cgtattgttt gtcatctatc
     5701 tgaaatatcg gatgaaatcg ttgaaatgta aagtattgag cacatccttt ttcgaaagcc
     5761 acccgcgtcg ggcagtggct actccgaact gcataaactc caaatcggat tcggtatgag
     5821 aatcagtgga gatgacaagt tttatcccct tttctttcgc ctttctgcag ttaatgtcat
     5881 tcaaatccaa tctttgagga taagcgttaa gctccaaaaa ttttctatta gccaagaccg
     5941 catcaaaaag cttgtccata cccacctcat acggctctct ttttccaatc agtcttccag
     6001 tcggatgggc gatgatatca acatttggat tttccactgc ccttattatc ctcttggtca
     6061 tctcttcctt ggactgactg aatttagtgt gaactgatgc taccaccaca tccaactttt
     6121 tcaaaatgga gtccgggaga tcaagctttc catcccctct aatatccacc tcaattcctt
     6181 tcaagaccac aattccttcc aatttttcgt tgagcttgtc gatctctttc atctgcctct
     6241 cgagcctttt ctcatccagg ccattggtta ttcccacata aggggagtgg tcacagatag
     6301 ccatatattc atatccgatt ttcttggcat accttaccat atcttcaatt gttctctttc
     6361 cgtctgacca attactgtga acatgcaaat cccctttgat atctttaagc tcaattagct
     6421 tgggaagtct gttttcgaaa cccgcctcta tttccccatt gtcttctctt atctcgggag
     6481 ggatataggg caagcccaga gcagaataca cttcttcctc agttcttcca gcaattctct
     6541 ttttcccttt gaaaataccg tattcactta cttttagccc tctggcttgc gctattgacc
     6601 tgagcttaat attatgagct ttggagccgg taaaataatg tgaagcggaa ccaaaacttt
     6661 cgggcttaac tattcgcaaa tcaacttgaa tattattttc aatctgaatg gaggatttgg
     6721 tgctcccttt agctaaaact tttttcactt gagggatttt gacaaaagca tctataattg
     6781 gttttgattt ttcagctgaa gctaaaatat cgatatccgc tatggtctct ttcattctcc
     6841 ttaaactgcc agctaagata gctttcttga cttgcttcat tttttccact tctttcaaaa
     6901 ccgcttgggc aaggggatag acaaaagcca gaggcattct ctctttgtgt tttatggctt
     6961 gctttatccc ttgaagtatg ttctgttcaa cttttggtcc aagtccgggc agttctctta
     7021 atcttccagc tcgggctgct ttttccaact gctttgttgt ccttatgtgt agttggtcat
     7081 aaaccaactt ggcgtgcttc ggtcccattc ccggaacttt cagcagttca atcaaaggag
     7141 catattcaga tttcttgagc ttctcgtaga atttcaatct accagtcttt aggatttctt
     7201 ttatcttctc aagtatggca tccccgattc caggaagctc tttaagttgg tcgatgtccg
     7261 gcatatcgga aatttgttcg gtgagatttt cgatagtttg gcccgctcta atataggctc
     7321 ttatgcggaa ggggttttca ccttgaatat ctaagatttc agcgatgcta taaaatatct
     7381 gggcgatttc acgattattc attttgagta tttcttgtag ttgcccaatt cattgggcga
     7441 tttttaaaaa acgctcgata aatgaggtta ctgaaaaacc cctttcgcag ggctgggagc
     7501 cctgcctacg ggagcgtaac tcattgattt ttcgtagggc tgggcacctt gcccagctga
     7561 tttctatgtt tgcacaggga taaattccct gtgctacgaa attaaaatat cgtaggtgcg
     7621 ggacaggccc acaccgagca gaatcgtaga tcgggttgcg actcccatct ttgacgggag
     7681 agctactcga ctatattaag ttcttatcgg gcatcctggg gaaggccttc ttacttacca
     7741 ttgaaatccc cctttatccc cctttgctaa agggggaact gttagattga ttctttcttc
     7801 ccctctttgt aaaagaggcc cgcttcgccc atctcgccga tggctcgccg aggcgggccg
     7861 aatcgaggcg agggttaggg gagatttcta ctcctgcatg tttctgtcag gagtcctagg
     7921 tcgggttgcg actcccagga gctacccgac agaatttttc tgtgagcggc agtttcattt
     7981 tggctttctc cccaagaact ccaatgcctt gctcaataca ggatcgttgt gtcgcaagta
     8041 gtcctgtact gtagggatca cttcaatatc aggtttcact ccatacccaa caaattctct
     8101 tccatcggga taagtattct ttttcgtgca gacccatgct tctcctcctc cgggtaactc
     8161 aaaaatgtac ggctgtccgg tactgccata tgaattctct ccgattttga ccatatgttt
     8221 ttgattgtca gcataaatta ggaaatcttc tgcagccgag gcagtgttat ggccaataag
     8281 gagcacagtt ggcacaacga cttttttctc gagcacgtgg atcgtatcgg gcgaataatc
     8341 aaacgtgtag tacgctttgt cctgaaaatc cagaaggcat ttctttgccc aatcgttgcc
     8401 tgtagtatcc tctggtttga catccgcacc ccatgctttg taggcaggaa tgtgcattct
     8461 actgaagcac ctataaccgt aaagaagggt atccggggtc aggtactgaa gaatctgttt
     8521 accaataggt tcgttcccac caccgttgtt gcggagatcg ataatgagcg ccttagcttt
     8581 gtacagatcc ggtaagtgct gctcaaacag cgtgtcgatc tttgggtcac caaaggagtt
     8641 taacgccagg taagcaatct gatgatcgta ccacttgaat tgcaggagct cataaagcgt
     8701 gtcctctttc tcaaacggag gatacacctc ctgttcactt gttcgttcat gggttagcaa
     8761 aaactgtttc agcttgccgt tcggagtctt gagggtaatc tcaaagcaat cccccttgag
     8821 tccttgcagt aagttggaga cggaaaggtc ctgtaggacg taactggtgg aagaggagaa
     8881 gtagggggcg acgaattgtg
//
LOCUS       DASWLN010000034         9961 bp    DNA     linear   ENV 20-FEB-2024
DEFINITION  MAG TPA_asm: Candidatus Zixiibacteriota bacterium isolate
            SMAG_U2949 k141_541183, whole genome shotgun sequence.
ACCESSION   DASWLN010000034 DASWLN010000000
VERSION     DASWLN010000034.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37960594
            Sequence Read Archive: SRR10376091
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Candidatus Zixiibacteriota bacterium (soil metagenome)
  ORGANISM  Candidatus Zixiibacteriota bacterium
            Bacteria.
REFERENCE   1  (bases 1 to 9961)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 9961)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (26-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/12/2024 08:28:26
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 1,440
            CDSs (total)                      :: 1,416
            Genes (coding)                    :: 1,412
            CDSs (with protein)               :: 1,412
            Genes (RNA)                       :: 24
            rRNAs                             :: 1 (16S)
            partial rRNAs                     :: 1 (16S)
            tRNAs                             :: 21
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 4
            CDSs (without protein)            :: 4
            Pseudo Genes (ambiguous residues) :: 0 of 4
            Pseudo Genes (frameshifted)       :: 0 of 4
            Pseudo Genes (incomplete)         :: 3 of 4
            Pseudo Genes (internal stop)      :: 1 of 4
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..9961
                     /organism="Candidatus Zixiibacteriota bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_541183"
                     /isolate="SMAG_U2949"
                     /isolation_source="soil"
                     /db_xref="taxon:2053527"
                     /environmental_sample
                     /country="USA: Oregon"
                     /lat_lon="42.99 N 123.63 W"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            <1..211
                     /locus_tag="VGB16_01195"
     CDS             <1..211
                     /locus_tag="VGB16_01195"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_013906818.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0016020 - membrane [Evidence IEA];
                     GO_process: GO:0009306 - protein secretion [Evidence IEA]"
                     /codon_start=2
                     /transl_table=11
                     /product="EscU/YscU/HrcU family type III secretion system
                     export apparatus switch protein"
                     /protein_id="HEX9916326.1"
                     /translation="PTVVAKGERLLAEKIKEVAIEYDVPIVEDRPLERAPYKAVKIGM
                     EIPANLYRAVAEVLAYVYGLKRRTK"
     gene            212..2293
                     /gene="flhA"
                     /locus_tag="VGB16_01200"
     CDS             212..2293
                     /gene="flhA"
                     /locus_tag="VGB16_01200"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_013120390.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0009288 - bacterial-type flagellum
                     [Evidence IEA];
                     GO_process: GO:0001539 - cilium or flagellum-dependent
                     cell motility [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="flagellar biosynthesis protein FlhA"
                     /protein_id="HEX9916327.1"
                     /translation="MADNISDNSLFQRALKHSDILLAAAVIGILAVLIIPVPTQLMDI
                     LLAFNIALSIVILLVTMYITAPLEISVFPGLLLIVTLFRLSLNVAATRLILGDAYAGE
                     VVNAFGNFVVKGNYVVGFVIFLILVVIQFVVITKGATRIAEVAARFTLDAMPGKQMAI
                     DADLNAGLIDDNEARRRRLEISREADFYGAMDGASKFVRGDAIAGILIALINIIGGLL
                     IGVVQKGMPISQALETYTVLTVGEGLVTQIPALVVSTGAGIIVTRAAAESNLGHDLIR
                     QLTLHPRAIMVACAMLFLFAIVPGLPTIPFLVLGCLAGVLAYTVNRAKKSEKELSKPK
                     EEVKQQTKPPEKPEEYLKIDTLEVEIGYGLIPLVDASQGGDLLDRVSIIRRQCAMDMG
                     IIVPPIRIRDNIQLKPNQYNFKIKGVAVAGGTLLMDHLLAISPGYVQEEIEGISTTEP
                     AFGLPAKWITRTVKELAESRGYTVVEPAAVLATHLTEIIKEHSHEVLSRQDVQNILDN
                     LKKDYPAVVGDVVPQILPLGTVQKVLQNLLKERVPIRDMVTILETLADYGSMTKEAEV
                     LAEYVRFALARSISQTYVNSENNIAVFTLDPQIEQLLAESAQTTKLGINVVMQPEMAQ
                     KLIKKVSAQVDSMITKGHQPIALVSPNVRLVFKRFIESSLPNLVVLSYNEIAPNFEVY
                     SLGVVGLEDAD"
     gene            2325..3464
                     /locus_tag="VGB16_01205"
     CDS             2325..3464
                     /locus_tag="VGB16_01205"
                     /inference="COORDINATES: protein motif:HMM:NF012662.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEX9916328.1"
                     /translation="MKKIKQEMGASALILDTKVAAKGDPYGLPNGNRVEITAAIDHHP
                     RKPALAQTERKVGTPAIFPKIKLVKPTESSQESLKETVKPAQSSSEIVGINESISNLK
                     NQIDLLGQELEHKRTLNNLPDSLKQLYSKLEDADFDEEESKKIILELDSRLALSGEDN
                     SEIIVQELKQTIQKLINLCSLLPSNQNAPIKLLFVGPSGDGKTTSLEKMAYQLAVKEG
                     RKVGLVTLDVERIGAVPSLVSYACILGVRCAGVYDLIELGGCLEKFKDLDVILFDTRG
                     INPWIKSELKELADWTSEIRPDKIFLVLSANHRYKDLIEMTYRFLCLGNADLLFTKLD
                     QTKRYGGILSCAIKTRRPISFLGWGTSVTEDFEKADLSRITDMMF"
     gene            3476..4327
                     /locus_tag="VGB16_01210"
     CDS             3476..4327
                     /locus_tag="VGB16_01210"
                     /inference="COORDINATES: protein motif:HMM:NF013793.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="AAA family ATPase"
                     /protein_id="HEX9916329.1"
                     /translation="MLTRQEEIISRMMKDDTPEVSVQTFAISSGKGGVGKSFITLNLG
                     LKLTQLNQKVLILDTDLGLANISLMANLNPTHTLEDVLNGKRSLDEAITTYSNLHILP
                     ALSDLSGFDMHSDEKIDRLQRSLSEVAKNYDFVLIDTQASLSPFTLSFLLGSDKMLLV
                     LTPQPTSIANGYALLKFILSKDSEKDIALLWNMVDSEKEVCQLKEKFNLLCDNFLGVK
                     VKEAGYLSNDEKVGSSINKQMPLIIRYPFSQAATCLERIALRMINDKRNIEKVELGML
                     DRGQGRG"
     gene            4447..5205
                     /locus_tag="VGB16_01215"
     CDS             4447..5205
                     /locus_tag="VGB16_01215"
                     /inference="COORDINATES: protein motif:HMM:NF005413.0"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003677 - DNA binding [Evidence IEA];
                     GO_function: GO:0003899 - DNA-directed 5'-3' RNA
                     polymerase activity [Evidence IEA];
                     GO_function: GO:0016987 - sigma factor activity [Evidence
                     IEA];
                     GO_process: GO:0006352 - DNA-templated transcription
                     initiation [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="FliA/WhiG family RNA polymerase sigma factor"
                     /protein_id="HEX9916330.1"
                     /translation="MSSEEVWVLYRKSKNPELRQRLLAEYLPLVKSVAGRMAMGFPRS
                     VELSDLVSTGVIGLIEAFGNFDPSRGVKFETYAVPRIRGAILDELRALDWVPRSTRAK
                     SRVIEKALVKLENKLGRIPAKVELARSLKITTSELYDALGDACGSTVLSLDELVYKED
                     DNRQVPRVETVGSVQEEGVLGQIEHCELKEFMVSAIGHLTEQERLVVTLYYYEELTLK
                     EIGEVMQISESRVSQIHTKAIMKLRSMIRERFPI"
     gene            5224..5466
                     /locus_tag="VGB16_01220"
     CDS             5224..5466
                     /locus_tag="VGB16_01220"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEX9916331.1"
                     /translation="MVRPVDLNDSYLQAEVAEKIHQIKKADPETEKKQFDKELAKKLK
                     EQKKPASSQDETTQGDVNQEQKEAEDSKSDHINMTV"
     gene            5494..6690
                     /locus_tag="VGB16_01225"
     CDS             5494..6690
                     /locus_tag="VGB16_01225"
                     /inference="COORDINATES: protein motif:HMM:NF014567.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ATP-binding protein"
                     /protein_id="HEX9916332.1"
                     /translation="MKVTDKERELAISSKEKRGLTSELKGFLGSLVELWGQMLEFENC
                     GVFLWGEDGQGMIPMDWVHRCEDYLDKFKGEWIRQIADQLAVEKKISVVRDLSDHRKS
                     LIFAPFKAGEKLGALVIWSGKSKESFTAKELEIFSFLAEQLNLYAESLHLQDKLSQLS
                     SWLKNREKYLEGIGKLAAVGELTAGVAHDINNPLQIILGKTQLLMMRLSRLSGNNKHI
                     DALQVIEKNATRISTVIKELADFARGKGEEGGLSSDVNLKHALKLAHSLVKSRFEAQN
                     VKLSLKIENNLPSIKGDVNQIEQLLLNLLLNAKDSVPEGGRLEIDVKRDGDFVKLKFK
                     DSRPPIPPEEVSQIFEPFACVGKSKGLGLGLYICSQIVTKHKGEIKVTSNPDEGTVFS
                     IRLPVI"
     gene            6709..7083
                     /locus_tag="VGB16_01230"
     CDS             6709..7083
                     /locus_tag="VGB16_01230"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEX9916333.1"
                     /translation="MLSAPFYFSLGLGVSLFFVVLFLLLVYISIRLRKNRPIQSQLQT
                     RPKSVSAQPKLNARIGQKSPMVVANSRTNNSFAYGLSPDFKVETYNRVKRLLEKKEDI
                     KTIARKEHLSLGEVRLIANLSE"
     gene            7123..8286
                     /locus_tag="VGB16_01235"
     CDS             7123..8286
                     /locus_tag="VGB16_01235"
                     /inference="COORDINATES: protein motif:HMM:NF021501.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF2225 domain-containing protein"
                     /protein_id="HEX9916334.1"
                     /translation="MTKLECPICGSVNEIENIKVGAYVEGGRETDFAPRDIIWANPDY
                     QTTNPLLYFMATCSHCFYTHEFNKEFKEWKNDNTFKMYRLKGLREKHLKKLAEPDSLI
                     RTMGSAIDAKHYPFQSAIIKFLLGIYDELFLERPSALDLGRYYLRIAWLFRENQKGMT
                     TVHQAEKMSLANWGKTVRGLVAAGQDLKSRLDKLKSVVDAQFKMKSSDEEQNTKREAM
                     RNDYLKIMEELNSLVSHLDRKSDELLSVYARSEKLVFDQGDKSFKLAEGFKSFPSFEA
                     FFSSLLKEWREIPTNEYEAIKLSLKYYKQSFEQNRDINPGNQQIQATYIISELSRRVG
                     DYEEARKYFNVAIRVGQEFINKNRDDVNKTALARKILELALEQGKLNLAEAQS"
     gene            8308..9051
                     /locus_tag="VGB16_01240"
     CDS             8308..9051
                     /locus_tag="VGB16_01240"
                     /inference="COORDINATES: protein motif:HMM:NF018894.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0035438 - cyclic-di-GMP binding [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="PilZ domain-containing protein"
                     /protein_id="HEX9916335.1"
                     /translation="MSTLKEYRNPDGVGFKLEDKFELEVKNSSPLRGFFAVVKEVSDF
                     GVILSDAVLNKGEGKLEKGDEIIVRFPDKGVVYEFDSEVLSQKGEGVYLVKAPLVIRR
                     SQRRRYVRLDISAPISFRLLCLKDNDCLVSKAEYSGVILNIGGGGILLVTKRKLEEGD
                     FVIVDMSLEGCEVISGVLGRVKRSEQSSEDEFLIGVEFCPQEELEYYLTSEQIAQLPV
                     RVKSFNQRLREVLSKYIFYQQATPKEEGH"
     gene            9056..9394
                     /locus_tag="VGB16_01245"
     CDS             9056..9394
                     /locus_tag="VGB16_01245"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEX9916336.1"
                     /translation="MKAVKFQSTYGSVTLACLLLLVMALTSSGQEGFKLVIPRFDKGT
                     FVSSDQYVLDRFMEILAAGSKTEIVGPELAKYELEKQRTELGFRFSTGGYAGGGQGIR
                     GRFGFMGRDG"
     gene            9472..9687
                     /locus_tag="VGB16_01250"
     CDS             9472..9687
                     /locus_tag="VGB16_01250"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEX9916337.1"
                     /translation="MSLKIFDLKKGEMVLAKSFDAFAFGDKGIAWFVHPEKEPDRRLS
                     PLEQDKLLREAEDKMATDMALRLSLSF"
     gene            9711..>9961
                     /locus_tag="VGB16_01255"
     CDS             9711..>9961
                     /locus_tag="VGB16_01255"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEX9916338.1"
                     /translation="MKRYQQRDAELELEVLSEMSNLVRTCSTDEENFEKMLKLMGRCV
                     EYSSASLFLVNRQSGQLDNVAYVGKRVDLIDFVKFDMGQG"
BASE COUNT         2908 a         1898 c         2568 g         2587 t
ORIGIN      
        1 ccccacagtc gttgccaagg gagaaagact tttagctgaa aagataaaag aggtagccat
       61 tgaatatgac gttcccattg ttgaagatag gcccctggag cgtgcccctt ataaggcagt
      121 taagattggg atggagatac ctgccaacct gtatcgggct gtggcagagg ttttagctta
      181 tgtctatggt ttgaagagga gaacaaaata agtggctgat aacatttccg ataattcact
      241 ttttcagcga gcattgaagc acagcgatat cctcttggct gcagcggtaa taggaatact
      301 tgcagtcctg attattccgg ttcccactca gttgatggat atactcttag cattcaacat
      361 cgccctctcc attgtgattc tcttggtgac aatgtacatt actgctcctt tagagatttc
      421 agtctttcca ggattgcttt tgatagtcac cctgttccgc ctttccttga acgttgccgc
      481 cacccgcttg attctgggag atgcttatgc cggtgaggtg gttaacgcct ttggaaattt
      541 tgtggttaag ggaaactacg tggttggatt tgtcatcttc ttaatcttag tagtcatcca
      601 atttgtagtc atcaccaaag gtgctacccg cattgctgag gtggcagcca ggttcacttt
      661 ggatgctatg cccggaaagc agatggccat tgatgcggat ttgaatgctg gcctaatcga
      721 tgataatgaa gcccgtcgaa gaaggttgga aatctccaga gaggcggatt tctatggagc
      781 tatggatggt gccagtaaat tcgtgagggg agatgccatc gccggaatcc tcatcgcttt
      841 gataaatatc atcggcggac tattgattgg ggtggtgcaa aaagggatgc cgatttccca
      901 agccctcgag acttataccg ttttgaccgt gggagaggga ttagttactc aaattccagc
      961 cttagtggtc tccaccggcg caggaattat tgtcacccga gcggcagcag aatcgaattt
     1021 aggtcatgac cttattcgtc aattgaccct acatccgcga gctattatgg ttgcctgtgc
     1081 catgctcttc ctatttgcca ttgtgcccgg gcttcctaca attccatttt tagtgttggg
     1141 atgcttagct ggagttctgg catatacagt taatcgcgca aaaaagagtg aaaaagagct
     1201 atcaaaacct aaagaggaag taaaacaaca aaccaaacct ccggagaagc cagaggaata
     1261 cttaaagatt gataccttag aagtggagat tggttacggg cttatccctc tggtagatgc
     1321 ttctcaaggt ggagacctct tggacagggt ttcaattata cgccgacagt gtgctatgga
     1381 tatggggata attgttcctc caatccgtat acgcgataac attcaactca aaccgaatca
     1441 gtataatttc aaaattaagg gagttgcagt ggctggcgga acccttttga tggatcatct
     1501 tttagccatc agcccggggt acgttcagga agaaattgag ggaatttcca ccacggagcc
     1561 ggcatttgga ttgcctgcca agtggattac ccgaacagtt aaagaattgg ctgaaagcag
     1621 gggctacact gtggttgagc cagctgcagt cttagctacg catttaactg aaattataaa
     1681 agagcactcg catgaagtgc tttcccgaca agatgtgcag aacattttgg ataacttgaa
     1741 aaaggattat cccgcagtgg tgggtgatgt ggtgccacag attttgcctt tgggcacggt
     1801 gcaaaaagta ttgcagaatc tcttgaagga gagagttccc atccgcgata tggtgaccat
     1861 cctcgaaact ttagcggatt atggtagtat gaccaaggaa gccgaggtct tagcagaata
     1921 tgtgcgattc gctttagcac gcagtatcag tcagacttat gtcaattccg aaaacaacat
     1981 cgcggtcttc actttggacc ctcagatcga acaactcttg gcggaaagcg cacagacgac
     2041 caaactcgga ataaatgtgg tgatgcagcc ggagatggca cagaaactaa tcaagaaagt
     2101 ttcagctcag gtggatagca tgataaccaa gggacatcaa cccatcgctt tggtctcgcc
     2161 caatgtgaga ttggttttca aacggtttat cgagtcgtcc ttacctaacc tggtggtcct
     2221 ttcctataat gaaatagcac ctaactttga agtctattcc ttaggagtgg tgggtttaga
     2281 agatgctgat taaaaaattc gttgggacaa gtgtcccgga gactatgaag aaaatcaagc
     2341 aggagatggg agcatccgct ctgattctgg acaccaaggt tgctgccaaa ggcgacccct
     2401 atggccttcc caatgggaat cgggtggaaa tcactgcggc aattgaccac catcccagaa
     2461 aacctgccct agcccaaacg gagagaaaag ttggaacgcc agctattttt cccaagataa
     2521 aattagtcaa gccaacagaa tctagtcaag aatccctgaa agagacagtt aagcctgccc
     2581 aaagctcttc tgagattgtc ggtataaacg agagcatatc caatttgaaa aaccagatag
     2641 atttattggg tcaggaattg gagcacaaaa ggactctaaa taatctgccg gatagtctga
     2701 agcagctgta ctcgaaatta gaagatgctg atttcgatga agaggagtcc aagaaaatca
     2761 tactggaact agactctcga ttggctctgt caggagagga taattcagag atcatcgtgc
     2821 aggagcttaa gcaaactatt cagaagctga taaatctttg ttctctttta ccctccaacc
     2881 agaatgcccc aatcaagcta ttgtttgtcg gtccgtccgg agatgggaaa accaccagct
     2941 tggagaagat ggcttatcaa ctggcagtca aagaaggacg gaaagttggg ttggtgactt
     3001 tggatgttga gcgcattggt gcggtgccga gcttagtgag ttatgcttgc atcttgggtg
     3061 ttcgctgtgc gggagtatat gatttgattg aactgggggg atgcttggaa aagttcaaag
     3121 acttagatgt cattctcttc gacaccagag gaatcaatcc ctggattaaa tcggaattaa
     3181 aggagttggc tgattggacg agcgaaatca ggccagacaa aatcttctta gttctctccg
     3241 ccaaccatcg atataaggac ctaatagaga tgacatacag attcttatgt ctgggcaacg
     3301 cagacctgct tttcacgaaa ttagaccaga ctaaacgtta tggcggaatc ttaagttgtg
     3361 ctataaaaac cagaaggccg atctcgtttc tcggttgggg aacatctgtt accgaggatt
     3421 tcgagaaggc ggatttaagt aggattacgg acatgatgtt ttaggagcga gcgatatgct
     3481 aactcgacaa gaagagatta tttctcgaat gatgaaagat gatactccag aggtatccgt
     3541 tcaaactttc gccataagtt caggtaaagg tggagtgggg aagagcttca taactttaaa
     3601 tttgggattg aaactgaccc agctcaatca gaaagtttta atactggata cggatttggg
     3661 cttagccaat atctccctta tggctaatct caatccaacc cataccttag aagacgttct
     3721 aaatgggaag cgcagtttgg atgaagccat aacgacttat tcaaatctgc atatcttgcc
     3781 agccttatcc gatttatcag ggtttgatat gcattcagat gaaaagatag atagattaca
     3841 gaggagcttg tcagaagtag caaaaaacta cgatttcgtt ttgattgaca cgcaagctag
     3901 cctttcaccc tttacattgt catttcttct aggctctgac aaaatgcttc tagtcttgac
     3961 tccccagcca acttccatag ccaatggtta tgctttactg aagtttattc tctctaaaga
     4021 ttcggaaaag gatatagctc tgttgtggaa catggttgac tctgaaaagg aggtttgcca
     4081 attgaaggag aaattcaacc ttttgtgtga taacttctta ggagtgaagg tgaaagaagc
     4141 cggttacctg tcaaatgacg agaaagttgg aagctcgata aataaacaga tgcctctcat
     4201 cataagatac cctttttccc aagcggccac ctgtttggag agaatagccc ttagaatgat
     4261 aaatgataaa cgaaatatag agaaagtcga gctcggtatg ctagatcgag ggcagggcag
     4321 aggttaaata aatccaatta taagtcgata ttataattag gtactctgct ttaaaaccct
     4381 cacttattgt tagcaaagaa gatagaagaa aactaaaagg attttcacga ttcaagaggg
     4441 gtggatatgt caagtgagga agtttgggtt ctttatagaa aaagtaaaaa cccggagttg
     4501 agacagagac tcttggcgga gtatttgccc ttggttaaga gtgtggccgg gagaatggct
     4561 atgggctttc cgcgctcggt ggagttatcc gatttggtca gcacaggggt gataggactg
     4621 attgaagctt ttggtaactt cgatccctcg cgcggagtca agtttgagac ctatgccgtt
     4681 cctcgtatac gaggagcgat cttagatgag cttagagctt tggattgggt gccgcgctct
     4741 acccgggcaa agtcacgagt gattgaaaaa gctttagtca aattggagaa taaattggga
     4801 aggatacccg ctaaagtgga attggctcgc agtctaaaga tcaccacctc agaattgtat
     4861 gatgctctgg gtgatgcctg tggctccacc gttttgtctt tggatgaatt ggtctacaaa
     4921 gaggatgaca atcgtcaagt gccacgggtt gagaccgttg gatcggtaca ggaagaggga
     4981 gtactgggtc agatagagca ttgcgagtta aaggagttta tggtttcggc catcgggcac
     5041 ctgactgagc aggaacgctt agtagttacc ctttactatt atgaggagct gaccctgaag
     5101 gagatcggcg aggtgatgca aatttccgaa tcgcgggtct cgcagataca taccaaagcc
     5161 attatgaaat tgcggtcgat gatcagagag aggtttccta tttagaaaga cgggagaggg
     5221 gatatggttc gacctgtaga tttaaatgat tcctacttgc aagccgaggt cgcagagaag
     5281 atacaccaga tcaagaaagc tgatccggaa acggaaaaga agcaattcga taaagagttg
     5341 gcaaagaaat tgaaggaaca gaagaagcca gcttcatcac aggacgagac cactcaaggt
     5401 gatgtgaatc aagaacagaa ggaagcagaa gattctaagt cagatcacat caatatgacc
     5461 gtttagtccc gacaagggcg ggatggagga gaaatgaaag ttactgacaa ggaaagagag
     5521 ctagctattt cctcaaagga gaagagaggt ttaacttcgg aattgaaagg gtttctgggc
     5581 tctctggtgg aattgtgggg acagatgtta gaattcgaga attgtggtgt tttcctgtgg
     5641 ggggaagatg gtcagggtat gattcccatg gattgggttc atcgatgcga ggattatctg
     5701 gataagttca aaggggagtg gatcaggcaa atcgccgacc aattggcagt ggagaaaaag
     5761 atctcggtag tgagagatct atctgatcac cgaaagtctt tgattttcgc ccctttcaaa
     5821 gcaggcgaga aattgggtgc tttagttatc tggtcaggga aatcaaaaga aagctttacc
     5881 gccaaagagc tggagatatt ctccttcttg gcggagcaac taaatctgta tgctgagagc
     5941 ttgcacctcc aagataaatt gagccagctg tccagttggc taaaaaatag agaaaaatac
     6001 ttagaaggga ttgggaagtt ggcggcagtg ggggaattga ccgcaggagt ggcacatgat
     6061 atcaataatc ctttgcagat aatcctgggt aaaacccaac ttctgatgat gaggcttagt
     6121 cgattaagtg ggaataataa gcacattgat gcgttgcagg tgattgaaaa aaatgccacg
     6181 cgtatctcca cggtaatcaa agaactggct gatttcgccc gtggcaaggg agaagaaggc
     6241 ggattgagct cagatgtcaa tctcaaacat gccttgaaat tggctcactc tctggtgaaa
     6301 tcacgtttcg aagcccaaaa tgtcaaactc agtctgaaaa tagaaaacaa tcttccctcc
     6361 ataaagggtg atgtcaatca aatcgaacaa ctgctactca atctgctttt gaatgctaag
     6421 gattctgtgc ctgagggagg aagattggaa atagatgtta aaagggatgg tgatttcgtc
     6481 aagctgaaat tcaaagatag tcgtccgccc attccacccg aagaggtttc ccagattttt
     6541 gagccttttg cctgtgtggg taaaagtaaa gggttaggct taggacttta catttgcagt
     6601 cagatagtca ccaaacataa gggtgaaatc aaagtaacca gcaacccgga tgaggggacc
     6661 gtcttttcaa tcaggttgcc agtaatataa aagaggggtg aaggccaaat gctaagcgct
     6721 cctttttatt tttctttagg cttgggagta agcttgtttt ttgtggtgct gttcttgctt
     6781 ttggtctata tatccattcg tctcagaaag aacaggccca tacaaagcca actccagacc
     6841 agacccaaat cagtttccgc tcagcccaaa ttgaacgcca ggataggtca aaagagtcct
     6901 atggttgtgg ctaattcccg aacaaacaat tcatttgctt acgggttgag ccccgatttt
     6961 aaagttgaga cttacaatcg ggtcaagagg cttttggaga agaaagagga tatcaagact
     7021 atcgctcgca aagagcattt aagcctggga gaggtcagat tgatagctaa ccttagcgag
     7081 tagaaaagga attcagaagt gagcatggaa tcgccattat ttatgactaa gctagaatgc
     7141 cccatctgcg ggtcggtcaa cgaaattgag aatatcaagg tgggggccta cgttgagggc
     7201 ggtcgggaaa ccgacttcgc tccccgtgac ataatctggg ctaacccgga ttatcaaacc
     7261 accaatccct tgctttactt catggctacc tgttctcatt gtttctatac ccacgaattc
     7321 aacaaagagt tcaaagagtg gaagaatgac aataccttca agatgtatcg tctgaagggg
     7381 ctgcgagaga agcaccttaa gaaattggca gaaccggata gtttgattcg cactatggga
     7441 agcgctatcg atgccaagca ttaccccttt caatccgcca ttatcaagtt tctcctaggg
     7501 atttacgatg aactgttctt ggaaaggcca tccgctctgg atttaggcag gtactatctt
     7561 cgtatcgcct ggctcttccg tgaaaaccag aaggggatga ccacggtaca tcaggcggag
     7621 aaaatgagtc tagctaattg ggggaaaacc gtacgtggct tggtcgcagc tggtcaggac
     7681 ctcaagagta gattggataa actcaaaagc gtagtggatg ctcagttcaa gatgaaatca
     7741 tcggatgaag agcagaacac caaaagggaa gcaatgagga acgattatct caagataatg
     7801 gaagagctca attctttagt ttcccattta gatcgcaaat cagatgaact tctttcggtc
     7861 tatgctcgca gcgagaagtt ggttttcgat caaggtgata agagcttcaa attggctgaa
     7921 gggtttaaga gtttcccttc gtttgaggcg tttttctctt ccttgctcaa ggaatggcgg
     7981 gagataccaa ccaacgaata tgaggctatt aaactgtctt tgaagtatta taaacaaagc
     8041 tttgaacaga accgggacat aaatccgggc aatcagcaaa tccaggctac ttacattatc
     8101 agtgaactct cccgaagagt gggggattac gaggaagcca gaaagtattt taatgtggcg
     8161 attcgagtcg gacaggaatt tatcaataag aatagagatg atgtgaataa aaccgcctta
     8221 gctcgaaaga ttctagaatt agctcttgaa cagggaaagc taaatctggc tgaggctcaa
     8281 agttaaactt aaggaactca aaacaacatg tcaaccctta aagaatatcg taatccggat
     8341 ggagttggct tcaaactgga agataaattc gagttggagg tgaaaaactc ttcccctttg
     8401 aggggcttct ttgcggtggt aaaagaggtc tccgatttcg gggtaatcct gtcggatgcg
     8461 gtattgaaca aaggcgaagg caagttggag aagggagatg aaataatcgt gcgttttcca
     8521 gataagggag tggtgtatga gtttgattct gaagtcctaa gtcagaaagg ggaaggggtc
     8581 tatttggtca aggctccatt agtgatcaga aggagtcaga ggagacgtta tgttcgcttg
     8641 gacatttcgg ctccaatcag tttccgcctg ctttgcttga aagacaacga ttgccttgtt
     8701 tcaaaagcgg aatatagtgg ggtgatactg aatatcggtg gcggaggaat ccttttagtg
     8761 accaagagaa aactagaaga gggggatttc gtaattgttg atatgagctt ggagggatgc
     8821 gaagtcatct ccggagtctt gggcagagtc aaacgttctg agcaatcaag tgaagacgaa
     8881 tttctcattg gtgtggagtt ttgtccgcag gaggaattgg aatattattt gacctcagag
     8941 cagatagccc agttgccggt aagagtgaaa agtttcaatc aaagactgag ggaggttttg
     9001 tccaagtata ttttctatca gcaggcaaca cctaaagagg aagggcattg aagaaatgaa
     9061 ggcagttaag tttcagagca cttatggaag tgtaactctc gcttgccttt tgttattggt
     9121 tatggcactc acctcttccg gccaggaggg tttcaagtta gtcattccca ggttcgataa
     9181 gggaaccttc gtttcctccg accagtatgt actcgatcgc ttcatggaga ttctggcggc
     9241 gggaagcaag accgagatag ttggtccaga attagctaaa tatgaattag aaaaacaaag
     9301 gactgaactt ggattccggt tctcgaccgg aggatatgct ggaggtgggc aaggaattag
     9361 ggggcgattt ggttttatgg ggagggatgg ataaaaagga gataaccatg aagcgggggg
     9421 ttatccgttc catacatttt tacaaagtac cagcatcaaa ctgaggtcaa agtgagcttg
     9481 aaaatattcg atttgaaaaa aggggagatg gttttggcca agagcttcga tgctttcgcc
     9541 tttggcgata aaggcatagc ctggttcgtt catccagaaa aggaaccaga ccgtaggctt
     9601 tcgcctttag agcaggataa actcttgagg gaggcggagg ataagatggc aacggatatg
     9661 gctctaagat tgtccttaag cttctgaaaa agggaaggta acatattgtc atgaaaagat
     9721 atcaacagag ggatgctgaa ctcgaactgg aagtgctatc cgagatgtcc aatctagtca
     9781 ggacttgttc cacggatgag gagaattttg agaagatgct caagcttatg ggaaggtgcg
     9841 tggaatacag ctctgctagt ttattcttgg tcaaccggca aagcgggcag ctggataacg
     9901 tagcctatgt tgggaagagg gtggatttaa tcgatttcgt gaagttcgat atggggcagg
     9961 g
//
LOCUS       DASWLN010000035         3563 bp    DNA     linear   ENV 20-FEB-2024
DEFINITION  MAG TPA_asm: Candidatus Zixiibacteriota bacterium isolate
            SMAG_U2949 k141_286447, whole genome shotgun sequence.
ACCESSION   DASWLN010000035 DASWLN010000000
VERSION     DASWLN010000035.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37960594
            Sequence Read Archive: SRR10376091
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Candidatus Zixiibacteriota bacterium (soil metagenome)
  ORGANISM  Candidatus Zixiibacteriota bacterium
            Bacteria.
REFERENCE   1  (bases 1 to 3563)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 3563)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (26-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/12/2024 08:28:26
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 1,440
            CDSs (total)                      :: 1,416
            Genes (coding)                    :: 1,412
            CDSs (with protein)               :: 1,412
            Genes (RNA)                       :: 24
            rRNAs                             :: 1 (16S)
            partial rRNAs                     :: 1 (16S)
            tRNAs                             :: 21
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 4
            CDSs (without protein)            :: 4
            Pseudo Genes (ambiguous residues) :: 0 of 4
            Pseudo Genes (frameshifted)       :: 0 of 4
            Pseudo Genes (incomplete)         :: 3 of 4
            Pseudo Genes (internal stop)      :: 1 of 4
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..3563
                     /organism="Candidatus Zixiibacteriota bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_286447"
                     /isolate="SMAG_U2949"
                     /isolation_source="soil"
                     /db_xref="taxon:2053527"
                     /environmental_sample
                     /country="USA: Oregon"
                     /lat_lon="42.99 N 123.63 W"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            <1..360
                     /locus_tag="VGB16_01260"
     CDS             <1..360
                     /locus_tag="VGB16_01260"
                     /inference="COORDINATES: protein motif:HMM:NF012488.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="aminotransferase class V-fold PLP-dependent
                     enzyme"
                     /protein_id="HEX9916339.1"
                     /translation="RIRKLPSHEYELNKILTDALEEYHQSGIIEILGPLEPRKRAGIL
                     TFEADQAALNEITEKCLASNVMFRAGNFCVDSFCELRRRPGRVRLSVYLYNNIEDCNM
                     FLEIAKPVLEKEFLARS"
     gene            complement(607..2268)
                     /locus_tag="VGB16_01265"
     CDS             complement(607..2268)
                     /locus_tag="VGB16_01265"
                     /inference="COORDINATES: protein motif:HMM:NF018530.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008324 - monoatomic cation transmembrane
                     transporter activity [Evidence IEA];
                     GO_process: GO:0006813 - potassium ion transport [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="TrkA C-terminal domain-containing protein"
                     /protein_id="HEX9916340.1"
                     /translation="MHSIINLLAASPLVLLFTVIGLGYFVGNIKVFGFKLGVAAVLFI
                     GIAFGAIDQRLALPEYIYVIGLVMFVYAIGLQAGPSFFASFQKRGLRTSIIAVLLLGL
                     GAAVAVILGKALGLSAPSIAGTFCGALTNTPALAATIETIKNLSPGLPQNLVKMYSQS
                     PVVTYGLAYPFGVFGVIFWFFILSKIFKIDFAKERGNLLKESTSSSILSKTYKATNPA
                     VIGKTVDQALHSLGHPGFVLSRIKKGNKIDMVADETTLSFGDLVVAVGTAENLERASI
                     LFGEEVKEQVEERRDDTSYHRYFVSNKKVVGKTISELHLRKEFEGTITRLQRGDVDFV
                     PTPDTTLELGDRVLVVSPREQTDHIKIFLGDSVQAISETDYLSLSLGIVLGAFVGMIP
                     LPLPNGMTFKLGFAGGPLVVGLILGKLERTGPIVWALPFNANLVLRQIGLVFFLAAIG
                     TKAGFGFGATFRSGGWGIIAVGAAITSFVAVATILVGYRFLKLPMSAIMGMVSGMQTQ
                     PACLAYANQQSQNDLPNLWYATVYPASMIAKIILAQIIVTTLLML"
     gene            complement(2289..>3563)
                     /locus_tag="VGB16_01270"
     CDS             complement(2289..>3563)
                     /locus_tag="VGB16_01270"
                     /inference="COORDINATES: protein motif:HMM:NF014598.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0016020 - membrane [Evidence IEA];
                     GO_process: GO:0009267 - cellular response to starvation
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="carbon starvation CstA family protein"
                     /protein_id="HEX9916341.1"
                     /translation="LIYVVIAFTDITAQTFKWGTTEVAFGSGVAISSVLYLVLGVLMG
                     ISLYKFKLNLKIATLIFLPLVLGIVWLGPKFPQPVISFVSGISIKKWDMILLIYCFIA
                     SLVPMWILLQPRGYLGGWLLYLTILTGLLGALFGGFHIQYPAFNVKGLESLANGKLIF
                     PILFITIACGACSGFHAIVSSGTTSKQLLRESDARIVGYGAMLLEGLVAVLALATVMM
                     IPKGEAILKSDPNLIYANGLARYLALVGVNFNIAFPFALLAFATFVYDTLDVCTRLAR
                     YILQELLGWKTAVGAFLATFVTLLLPAIFLMLTKEKGYLVAWPIFGTSNQLLASLTLL
                     VISVWLIRTKRNAVYTIIPMVFMMIVTMWSLVLQVLPFLGSFPGIFSGKTLQPTILIS
                     GTLGIILFILGVWLIFEAINVLALKRATVKET"
BASE COUNT         1106 a          813 c          771 g          873 t
ORIGIN      
        1 cgaataagaa aattgccgag ccacgagtat gaattgaaca aaatcctgac tgacgcctta
       61 gaagaatatc atcaatccgg aatcatagaa atcctggggc cactggagcc gcggaagcga
      121 gcaggcatac ttactttcga agcagatcaa gcagccctaa acgagatcac ggagaaatgc
      181 ctggcttcaa atgtcatgtt tagagccggg aatttctgtg ttgattcatt ctgtgaatta
      241 aggaggagac cagggcgggt tcgtctgtcc gtttatcttt acaataatat tgaagactgc
      301 aatatgtttc tggaaatagc aaaacctgtt cttgaaaagg aattccttgc ccggtcataa
      361 cataaattcg gtttagagat ttaatagttt aaaagtctaa taagttctgt tgcatcaggg
      421 gttattcctg gtgcaatttg taagggcaga cccttgtgtc tgccctgttt tatattttct
      481 atgtctattt ctgtaagaac tttatggtgg gcaaccgcag agggtctccc ctacggttat
      541 tctgtcaaaa aatcacccaa gattaaaaag tcctcgggcg attcttattg tagacaaaaa
      601 tatcaattac aacatcaaaa gcgtagtcac tataatttgc gccaatataa ttttggcaat
      661 cattgatgct ggataaactg tggcatacca cagattgggc aggtcatttt gggattgctg
      721 gttagcataa gccagacagg caggttgggt ttgcattcct gataccattc ccataattgc
      781 cgacataggt aattttagaa atctgtatcc taccaagata gttgctacgg caacgaaact
      841 tgtgatggct gcgccaactg cgattattcc ccatccgccg ctccgaaaag tagcaccaaa
      901 tccgaacccg gcttttgtgc ctatggctgc aagaaaaaag accaaaccta tttgtcgcaa
      961 gaccaaattg gcattaaaag gcaatgccca cacaataggt ccggttcgtt cgagtttgcc
     1021 taaaataaga cctaccacca gtggcccgcc ggcaaatccg agcttgaaag tcatgccgtt
     1081 gggcaggggc agtggaatca tccccacaaa agctcccagc actatcccta aggaaaggga
     1141 aagataatca gtctcggaga tagcctgcac tgaatccccc agaaaaatct tgatgtgatc
     1201 agtctgctca cgtggagaga ccaccaatac tctatccccc agctcgagag ttgtgtcggg
     1261 agtgggaacg aagtcaacat ccccgcgttg aagacgggtt atggtacctt caaactcctt
     1321 gcggaggtgt aattcactga tagtctttcc aaccactttc ttgttagaca cgaaatagcg
     1381 gtgatagctg gtatcgtcac gtctctcctc aacttgctct ttgacttcct cgccaaaaag
     1441 tatagaggct ctttccagat tttccgcagt acccacagct acaactaaat caccgaagga
     1501 aagagtcgtc tcatccgcca ccatatctat tttattgcct tttttaattc ggcttaagac
     1561 aaagccggga tgacccaaag agtgcaatgc ctggtcaaca gttttgccaa taactgctgg
     1621 attggttgcc ttataggtct tgctcaaaat agaggaagag gtagattcct tcaagagatt
     1681 gcctctttct ttggcgaagt cgattttgaa aatcttgctg agaatgaaaa accaaaatat
     1741 gaccccgaat actccaaaag gataagccaa gccataagtt accaccggac tctgcgagta
     1801 catcttaact aaattttgcg gcaggccggg agaaagattc tttatggttt cgatggttgc
     1861 ggctaaagcg ggcgtgtttg ttagagcacc acagaatgtt ccggcaatgc tgggagcgga
     1921 aagacccagg gccttcccca ggataacggc gaccgcagca cccaaaccga gaagaagaac
     1981 tgctattata cttgtcctta gtccgcgctt ttggaaggac gcaaagaaac ttgggccagc
     2041 ctgaagccca atggcatata caaacatcac taacccaata acatagatat actcaggaag
     2101 agccaacctt tggtcaatgg cgccaaaggc gatgccgata aataggactg cagcaactcc
     2161 gagcttgaaa ccaaagactt tgatgttgcc aacaaaatat cccagcccaa taacagtaaa
     2221 tagcaaaaca aggggactgg cggctaacag atttataatg gaatgcatag tgttttttta
     2281 aatatcctct atgtttcttt gacagttgct cgttttaatg ccagcacgtt aattgcttca
     2341 aatatcaacc atacgcccag gataaacagg ataattccaa gggtgcctga aatgagaatt
     2401 gtaggttgaa gggtcttgcc actaaatatg ccaggaaacg acccaagaaa aggaagaacc
     2461 tgcaaaacca gcgaccacat tgtcactatc atcataaaga ccatcggaat tatagtatat
     2521 actgcatttc tcttggtgcg gattaaccat acggagatga ccaaaagagt cagactggca
     2581 aggagctgat tgctcgttcc aaaaatgggc caagccacca aatacccttt ttccttggta
     2641 agcataagaa atatagcagg cagaagtaaa gttacaaaag ttgccaagaa agcaccgact
     2701 gccgttttcc agccgagaag ctcctgtaat atatatctgg caagccgggt gcacacatcc
     2761 agagtatcat aaacaaaggt ggcaaaagcc agaagtgcga aagggaaggc gatattgaaa
     2821 ttcactccta ccagcgccag atagcgagcc agaccattag cgtagatgag attcgggtca
     2881 ctcttaagaa tcgcttcccc cttaggtatc atcataaccg ttgccagagc taaaacggca
     2941 accaggccct ccaatagcat cgcaccataa ccaacaatgc gcgcgtccga ttcccgtaat
     3001 agttgtttcg aggttgttcc ggagcttact atcgcgtgaa agcccgaaca agcaccacag
     3061 gcaatagtta tgaataaaat cggaaagata agttttccat tagccaagct ttcaagccct
     3121 ttgacattga atgccggata ttgtatgtga aatcctccaa ataaagcacc taatagtccg
     3181 gttaatattg taagatataa tagccatcca cctaagtatc ctcttggttg aagcaatatc
     3241 cacatcggca caagcgaagc aatgaaacaa tatatcagca aaatcatgtc ccacttcttt
     3301 atggatatac ccgatacaaa ggaaattacc ggctggggaa acttgggacc gagccatacg
     3361 atacctagca ccaagggtaa aaagataaga gtggcgattt ttaggttcag tttgaatttg
     3421 tatagagaaa tccccatcaa aaccccaagt actagataaa gaacagaact tatggctact
     3481 cccgacccaa aagcaacctc agttgtgccc cacttgaatg tctgggcagt gatgtcagta
     3541 aaggcaatga caacataaat cag
//
LOCUS       DASWLN010000036         5840 bp    DNA     linear   ENV 20-FEB-2024
DEFINITION  MAG TPA_asm: Candidatus Zixiibacteriota bacterium isolate
            SMAG_U2949 k141_416276, whole genome shotgun sequence.
ACCESSION   DASWLN010000036 DASWLN010000000
VERSION     DASWLN010000036.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37960594
            Sequence Read Archive: SRR10376091
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Candidatus Zixiibacteriota bacterium (soil metagenome)
  ORGANISM  Candidatus Zixiibacteriota bacterium
            Bacteria.
REFERENCE   1  (bases 1 to 5840)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 5840)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (26-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/12/2024 08:28:26
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 1,440
            CDSs (total)                      :: 1,416
            Genes (coding)                    :: 1,412
            CDSs (with protein)               :: 1,412
            Genes (RNA)                       :: 24
            rRNAs                             :: 1 (16S)
            partial rRNAs                     :: 1 (16S)
            tRNAs                             :: 21
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 4
            CDSs (without protein)            :: 4
            Pseudo Genes (ambiguous residues) :: 0 of 4
            Pseudo Genes (frameshifted)       :: 0 of 4
            Pseudo Genes (incomplete)         :: 3 of 4
            Pseudo Genes (internal stop)      :: 1 of 4
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..5840
                     /organism="Candidatus Zixiibacteriota bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_416276"
                     /isolate="SMAG_U2949"
                     /isolation_source="soil"
                     /db_xref="taxon:2053527"
                     /environmental_sample
                     /country="USA: Oregon"
                     /lat_lon="42.99 N 123.63 W"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            <1..413
                     /locus_tag="VGB16_01275"
     CDS             <1..413
                     /locus_tag="VGB16_01275"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=3
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEX9916342.1"
                     /translation="GILGVMMLLRLVPKIGWISRWPLSFIVGSTAGLYFITYLQSNIM
                     NQIRSTIIPIYVPGNLGISISNLIVFVGVFCGLVYFFFSREHKGAFGGAAKVGIWFLM
                     ITFGASFGFTVMSRMSLLIGRIDFLFGTWLGLIK"
     gene            448..1638
                     /locus_tag="VGB16_01280"
     CDS             448..1638
                     /locus_tag="VGB16_01280"
                     /inference="COORDINATES: protein motif:HMM:NF042826.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF6754 domain-containing protein"
                     /protein_id="HEX9916343.1"
                     /translation="MKKTKFIILIIPLILIFFNLSYPQEKPVPLPPIEVKAFDTPNDN
                     GHSLSVTWKLSPDDGAGKNNVVAYEILRAESPEGEFEVRGMVPSGENLFDDKGSREKE
                     DKNYLPSHKNYYYKIRAKADNIFSESEVSNPAFAYGQWYHTGKTPVLAGVIIFSIFAL
                     TFITLARKGKKLYIRPLPGIEAVDEAVGRATEMGKPILYVLGLGTASDIATIASFTIL
                     NRIAKITAEHRTTILVPCYDSVVMAAAQETVRAGYLEAGRPDIYKEENVFFVTSMQFA
                     YVAGVNGLMLREKPATNFYLGKFYAESLLLAETGSIAGSIQISGTDEIAQIPFFIAAT
                     DYTLIGEELYAASAYLGKEPVLLGTLKAQDWGKAVVIICLILGALAASFHFQFFLNLF
                     RVQL"
     gene            1693..2319
                     /locus_tag="VGB16_01285"
     CDS             1693..2319
                     /locus_tag="VGB16_01285"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_018198646.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEX9916344.1"
                     /translation="MRRTLPFALVTGVGFFMLVQYFIPKEEFEIAYEWILDWTIVIGC
                     FAMLLGLWSLTRVNISKVQRKTPGWGYSLIAIAGLVFMLLVGFIPGQKSLERGSTFML
                     LFEYIYIPIQATMFSLLAFYIASAAYRAFRARTVLATILLLSAIVIMLRLVPLGPLSA
                     VNQWLANFILTVPNMAAKRAFWIGVGLGVTATSLKILLGIERTYMGRD"
     gene            2365..3219
                     /locus_tag="VGB16_01290"
     CDS             2365..3219
                     /locus_tag="VGB16_01290"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEX9916345.1"
                     /translation="MNFYERMMNLDRRWIYLVIGLVVVITAIFPFDVPLYVTPEVRSI
                     YDYIESLNPGDVLMIGVDYAPEVMAELHPMTYAISRQCFKKDVKLLLCCLHQNGPGMV
                     EQVISQVSKEYNKVNGVDYVYLGYKPYPAITILAMGQNFRIPFPEDYYGKPLDSYPMM
                     RGLTNFSNVKAIINISGGSGTEYWINYANGRYNAKLAIGVTGVMTSDYYPFLQSGQIF
                     GLIGGMKGASEYEYLAEKAGYISKAKNQLSAYKTMPIQTTSHLVVIFFIVVGNIGFFM
                     SKKKKSRI"
     gene            3241..3897
                     /locus_tag="VGB16_01295"
     CDS             3241..3897
                     /locus_tag="VGB16_01295"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_020250985.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEX9916346.1"
                     /translation="MGTEEIIWNGVAAFLVLCIYTFLYKDNPFYKFAEHLVVGVSAGY
                     FAVILYYNGFVPKLITPLKQGQWVYIIPAILGMMMWTRFSKKWSWLSRFSLAFYIGSG
                     TGFAIPLYMQNYVLRQFSSSMLPMGFSSWALFCNILIIVGVLSALVYFFFSKEHTGIF
                     GGTAKIGIWTLMIGFGAGFGLTVMGRISLLAERVVFLREYLLELKYYLYLLFKYGLPL
                     "
     gene            3894..4781
                     /locus_tag="VGB16_01300"
     CDS             3894..4781
                     /locus_tag="VGB16_01300"
                     /inference="COORDINATES: protein motif:HMM:NF042826.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF6754 domain-containing protein"
                     /protein_id="HEX9916347.1"
                     /translation="MKKLCWKILLLPLFIYFIFFSYSLASATETSTETISANQWFNSQ
                     RINVFIILIVFFAFVLYFIQKAKRGGSLFVRRIAGLDAIEDAVGRATEMGKPILYISG
                     IEDMDNLQTVASMIILGNVAEMVAAYDVPLIVPVCLPFVVTVAEESVKQGYAKAGRPE
                     AYNRNNVRYLTSEQFAYAAGVNGIMLREKPAANFMFGSFYAESLLMAETGLSTGAIQV
                     AGTANIHQLPFFVVACDFTLIGEELFAASAYLSREPKLLGSLKASDLAKFLIMVMLVF
                     GVLLETFGITKFTYWFFVQ"
     gene            4982..5596
                     /locus_tag="VGB16_01305"
     CDS             4982..5596
                     /locus_tag="VGB16_01305"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEX9916348.1"
                     /translation="MRREVPIIICFLAGAFFIGNYFSPKLQPVAQVVNQWAIICIAFA
                     YVLGVANIFRVNSQVIARKGKDWVYKIVLVAGLILMICFGILGEVREGTAFNWVFMNA
                     IYPMQATMFALLAFYIASAAFRAFRIRSWEAALLGITAVLVMIGRVPLGELVWKGFPP
                     FTEWIMNVPQLAAKRAIMIGAALGAISTGIKVLIGLEKTYMGGE"
BASE COUNT         1520 a         1148 c         1348 g         1824 t
ORIGIN      
        1 ctgggatcct gggagtgatg atgcttttgc gccttgtccc gaaaatcggc tggatttccc
       61 gctggcccct ttcctttata gtgggttcaa ccgcaggcct ttattttatc acttacctgc
      121 agagcaatat catgaaccag attcgtagta ccattatccc gatttatgtg cctggaaacc
      181 tgggaatctc catctcaaat ctaatagttt ttgtgggggt tttctgtggc ctggtttact
      241 tcttcttttc tcgggaacac aaaggggctt ttggcggagc agccaaggtg gggatctggt
      301 ttctaatgat aacctttggc gcttccttcg ggttcacagt tatgtcccga atgtccctgt
      361 taattggcag aatcgacttt ctcttcggaa cctggttagg gttgataaaa tagactgaac
      421 ttttttatct aaaaattcga ggcaaagatg aaaaaaacaa aatttattat tttaataatt
      481 ccacttatcc tcatcttttt caatctatcc taccctcagg aaaaaccagt tcctcttcct
      541 ccaattgagg tcaaggcttt tgacactccc aatgataatg gacattccct ttctgttacc
      601 tggaagctct ccccggatga tggtgccgga aaaaataatg tggtggctta tgagattttg
      661 agagcggagt ctccggaggg ggaatttgaa gttagaggaa tggtaccatc cggagaaaac
      721 ctttttgacg ataaaggttc aagagaaaag gaagacaaaa actatctgcc cagccataag
      781 aattactatt ataagataag agcgaaagca gataatatct tttctgaatc tgaagtctcc
      841 aacccggctt ttgcctatgg acagtggtat catactggga aaaccccggt tttagctggg
      901 gtaatcatct tttctatttt tgccttaact tttataacct tagcccgtaa gggtaaaaag
      961 ttgtatatac gacccttacc cgggatcgag gctgtggatg aggctgtggg cagagccact
     1021 gaaatgggaa aacctatcct ctatgttctg ggtctgggta ctgcttcaga tattgctact
     1081 attgcctctt ttaccatcct gaatagaatt gccaaaataa ctgcagaaca cagaactacc
     1141 attctggtcc cttgctacga ttcagtggta atggccgccg cccaggaaac tgttagagct
     1201 ggatatctgg aagctggcag gccggatatt tacaaagagg aaaatgtctt ctttgtaacc
     1261 tcaatgcagt ttgcctatgt ggctggagtt aacgggttaa tgttaaggga aaagccagct
     1321 acgaatttct atttaggaaa attctatgcc gaatctttgc ttttagcaga gaccggctct
     1381 atagctggat ctattcaaat ctccggaaca gatgaaatcg ctcagatccc cttctttatc
     1441 gcggcaacag actataccct gataggtgaa gagctttatg ctgcctcagc ttatctggga
     1501 aaagagccag ttctcttggg aacccttaaa gcacaggatt ggggaaaagc agtagttatt
     1561 atatgtttga tcttaggtgc tctggctgcc agtttccatt ttcagttctt tttaaacctg
     1621 ttcagggtgc agctctaaga gcatagcctg gcgcccaaag ggcgggcagg ctcgtagcca
     1681 aaggagaaaa agatgcgtag aactttacct tttgctctcg ttaccggtgt gggattcttc
     1741 atgctggttc aatattttat tcccaaagag gagtttgaga tagcttatga atggatttta
     1801 gactggacca tcgttatcgg ctgttttgcc atgcttttag gattatggag cttaaccagg
     1861 gttaatatct ccaaggtaca gaggaaaacc cctggttggg gatatagttt aattgccata
     1921 gctggactgg tctttatgct gctcgtcgga tttatcccgg gacagaaaag cctggagcgt
     1981 ggtagcacct ttatgctcct tttcgaatat atctatattc cgatccaggc tactatgttt
     2041 tcacttttag ccttttatat tgcttcagcc gcctatcgag ctttcagagc ccgcacagtt
     2101 ttagccacca tacttctcct ctctgctata gtaataatgt taagattggt gcctctgggt
     2161 cctttatccg ctgtaaacca atggttggct aacttcatct tgactgtgcc caatatggcc
     2221 gctaaaaggg ctttctggat aggagtgggg ttaggagtta ctgctacctc ccttaaaatt
     2281 ttgttaggga ttgagagaac ctatatggga agagattagt cctttaatcc gcttcaggcg
     2341 gatacagctc gcagaggaga aaatatgaat ttctatgaac ggatgatgaa tttagataga
     2401 cgctggatct atctggttat aggattggtg gtggtgataa ccgctatttt cccttttgac
     2461 gtccctcttt atgttactcc ggaagtgcgt agcatttatg attatattga gagtttaaat
     2521 ccgggtgatg tcttgatgat tggagtcgat tatgcgcctg aggttatggc tgagcttcat
     2581 cctatgacct atgctatatc ccgtcagtgt tttaaaaaag atgttaagct acttttatgc
     2641 tgtcttcatc aaaacggtcc aggtatggtt gagcaggtga tctcccaggt atcaaaggaa
     2701 tataacaagg tgaatggagt ggattatgtc tatttaggat ataaacccta ccctgcaatt
     2761 acaattttag ctatggggca gaacttcaga atccctttcc cggaagacta ttacggcaaa
     2821 cctttggatt cttatcctat gatgagaggt cttaccaact tcagtaatgt gaaggcgatt
     2881 attaacatct ccggaggttc aggaacagaa tactggataa actatgcgaa tggtcgttat
     2941 aatgccaagc tggcaattgg tgtgaccggg gttatgacct cagattacta tccttttctt
     3001 caatccggac agatcttcgg attaatcggc ggaatgaagg gtgcttcaga atatgaatac
     3061 ttagctgaga aggctggata tatctccaaa gcaaaaaatc aattgtctgc ttataaaact
     3121 atgcctattc aaaccaccag tcatttagtg gtgatatttt ttatcgttgt gggaaacatc
     3181 ggatttttta tgtcaaaaaa gaaaaaatct cgaatttgag ccttgaggaa agggataatt
     3241 atgggaacag aagagataat ctggaacggt gttgccgcat ttttggtttt atgcatctat
     3301 acttttcttt ataaggacaa cccattttat aagtttgctg aacatctggt agtaggggtc
     3361 tctgcagggt attttgcggt gatcttatat tacaatggat ttgtgccaaa actgatcacc
     3421 cctttaaaac aagggcaatg ggtttatatc attcctgcaa tcctggggat gatgatgtgg
     3481 actcgctttt ctaagaaatg gtcctggcta tcccgtttct ccttagcttt ctatataggg
     3541 agtggcacgg gctttgcgat ccccctttat atgcagaact acgttttgag gcagtttagt
     3601 tccagcatgc tacctatggg attttcttcc tgggcacttt tctgcaatat cctgataata
     3661 gtcggagtcc tctcagcttt agtctacttt tttttctcca aagaacatac aggaatattt
     3721 ggggggacgg ccaagatcgg gatctggact ttaatgatcg gctttggagc aggatttggc
     3781 ttaacagtta tgggaaggat ctctctttta gctgagaggg tggtattctt aagggaatat
     3841 ctgttggagt tgaaatatta tctctatcta ttatttaaat atgggctgcc gttatgaaga
     3901 agctttgctg gaaaattctt ttactacctt tgtttattta ttttatcttt ttctcttatt
     3961 ctttagcctc ggcaactgaa acttctactg agaccatttc agctaatcag tggttcaatt
     4021 ctcaaaggat aaatgtcttt atcattttaa ttgttttctt tgctttcgtg ctctatttta
     4081 tccagaaggc taagagggga ggaagcctct ttgtgcgcag gattgcagga ctggatgcaa
     4141 ttgaagatgc agtgggaaga gcaactgaaa tgggaaagcc aatcctttat atctccggga
     4201 tcgaggatat ggacaatctc cagaccgttg ccagtatgat tattttgggg aatgtagcgg
     4261 agatggttgc cgcttatgat gttcctctca ttgttccggt ttgtctcccc tttgtagtga
     4321 cagtggctga ggaatcggtc aaacagggtt atgcgaaagc gggacggcct gaggcttata
     4381 atcgcaacaa cgtcaggtat ctgaccagtg agcagtttgc ctatgcagct ggagttaacg
     4441 ggataatgct tcgggaaaaa cctgcggcta attttatgtt tggttcattc tatgctgaat
     4501 cattgcttat ggctgaaact ggcctttcaa ctggagccat tcaggtagct gggactgcta
     4561 atattcacca gctccccttc tttgtggtag catgtgattt cacccttatc ggagaggagc
     4621 ttttcgccgc ctctgcctat ctatcccggg aacccaagct tttaggcagt ttaaaagcct
     4681 cggatttagc caagttcttg ataatggtaa tgttagtatt tggagtatta ttggaaactt
     4741 ttggaattac aaaattcacc tactggtttt ttgtgcagta gtggagttca acaacttgtt
     4801 gttgctaatt ttatatacgc aagagcaagc tcttgcactc cagaacaatg attaaaagca
     4861 gtaagacgtt agatgtgaga cgtaagacga taaaaagatg gagtgtaaat cttcaggttt
     4921 actgaaataa atggtcccgc caatggcggg accgctacaa aaaacactaa aagaaaatcc
     4981 tatgcgtaga gaagttccca taattatctg tttcttagct ggagctttct tcattggaaa
     5041 ctacttcagt cccaaacttc agccagtagc tcaggtagtt aatcaatggg caataatttg
     5101 cattgccttt gcctatgtcc taggagttgc caatatcttc agggtaaact ctcaggtaat
     5161 agccaggaag ggtaaagact gggtttataa aatcgtttta gtggcagggt taatcctgat
     5221 gatctgtttc ggaatcctgg gtgaagtcag ggaaggcaca gcttttaact gggtctttat
     5281 gaacgccatc tatcctatgc aggcgacaat gtttgccctt ttagcctttt atattgcctc
     5341 agccgccttc agggctttca ggataagaag ctgggaagcc gctctcttag gaattaccgc
     5401 agtcctggtg atgatcggga gggtgccatt gggagaactg gtctggaaag gtttcccgcc
     5461 ctttactgaa tggattatga atgtgcccca gcttgcggct aaaagagcca taatgattgg
     5521 agcggcttta ggcgccatct caactggaat caaagttctt atcggactgg agaaaaccta
     5581 tatgggaggt gaataacttt tttttgaagt ttttaattaa gtggtggtta tcagtcgggc
     5641 aagggtgccc gacctacgaa tagtcaaaat cttatgctcg taggcggggc tcccagcccc
     5701 gcaaaacggg tttttcggaa gtccctttat gagaaaattt tttgaaaaac ttgagaaatt
     5761 agaccgaagg ataatcttta tcttcgtagc tattgccctg atcttacccc tttttatccc
     5821 cttgaatatc aagttctcag
//
LOCUS       DASWLN010000037         4785 bp    DNA     linear   ENV 20-FEB-2024
DEFINITION  MAG TPA_asm: Candidatus Zixiibacteriota bacterium isolate
            SMAG_U2949 k141_35352, whole genome shotgun sequence.
ACCESSION   DASWLN010000037 DASWLN010000000
VERSION     DASWLN010000037.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37960594
            Sequence Read Archive: SRR10376091
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Candidatus Zixiibacteriota bacterium (soil metagenome)
  ORGANISM  Candidatus Zixiibacteriota bacterium
            Bacteria.
REFERENCE   1  (bases 1 to 4785)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 4785)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (26-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/12/2024 08:28:26
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 1,440
            CDSs (total)                      :: 1,416
            Genes (coding)                    :: 1,412
            CDSs (with protein)               :: 1,412
            Genes (RNA)                       :: 24
            rRNAs                             :: 1 (16S)
            partial rRNAs                     :: 1 (16S)
            tRNAs                             :: 21
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 4
            CDSs (without protein)            :: 4
            Pseudo Genes (ambiguous residues) :: 0 of 4
            Pseudo Genes (frameshifted)       :: 0 of 4
            Pseudo Genes (incomplete)         :: 3 of 4
            Pseudo Genes (internal stop)      :: 1 of 4
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..4785
                     /organism="Candidatus Zixiibacteriota bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_35352"
                     /isolate="SMAG_U2949"
                     /isolation_source="soil"
                     /db_xref="taxon:2053527"
                     /environmental_sample
                     /country="USA: Oregon"
                     /lat_lon="42.99 N 123.63 W"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            complement(<1..922)
                     /locus_tag="VGB16_01310"
     CDS             complement(<1..922)
                     /locus_tag="VGB16_01310"
                     /inference="COORDINATES: protein motif:HMM:NF015980.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEX9916349.1"
                     /translation="MKPKDLTNKILISIIPLMLVVFLYDTSCSQSEVHVKISTAGIEP
                     MDMAVIDFTPKNDKAVSQEKDLSKNITAVVRQDLLYALVFNLVEADSFASSILKGNWY
                     DFAGWFNLGAKTLVGGTVEAKKGTVIAEVDLWDVYRKRKISSEAYKTSVESYRALAHT
                     ISDDIVYKLTGEKGIFNSRIAFISDRTGYKEIYVCDYDGENQTQLTNHKSINLSPRWS
                     PDGKTIAYTSYKGGNPDLWLLSLKDNKSKVISAQAGLNSAPAWSPDGRTLAMTLSKDG
                     DAEIYLVNADGVIIRRLTKSSSIDSSPTWSP"
     gene            complement(925..1623)
                     /locus_tag="VGB16_01315"
     CDS             complement(925..1623)
                     /locus_tag="VGB16_01315"
                     /inference="COORDINATES: protein motif:HMM:NF024503.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0016020 - membrane [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="cell envelope integrity protein TolA"
                     /protein_id="HEX9916350.1"
                     /translation="MRRDVIISISFHLLVIIAIILFGPNIQPVKGYPKIYRVGLVSLP
                     APAGGSGEGGKTEVSGKAEKASEKGIAIKELKNSKEKKSISEKEQNAKKENLGKNKTG
                     SSKEQPKKERMPGGTTAGSSSGISGGIVGSAQVDGADFGGDYYVQMFVAKINDLWENP
                     IKSASSTLNAIVYFRILKDGDVVDVKIEKSSGVGVFDKAALRAVISVSPLQPLPNEYT
                     GEYLGVHLEFEFVQ"
     gene            complement(1620..2021)
                     /locus_tag="VGB16_01320"
     CDS             complement(1620..2021)
                     /locus_tag="VGB16_01320"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_012546534.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0022857 - transmembrane transporter
                     activity [Evidence IEA];
                     GO_process: GO:0055085 - transmembrane transport [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="biopolymer transporter ExbD"
                     /protein_id="HEX9916351.1"
                     /translation="MKKRTYSALHDINVTNLVDVVLVLLIIFMIAAPMLQSGIDVNLP
                     KTKAGQEDLGEGIVVTITKEKRIFIDDVVTQMGRFEQKLLDAKAKKRAKTVYLRADED
                     VPYGLVVEVIGKIKSLGIQDLGLVTQPVEKK"
     gene            complement(2018..2731)
                     /locus_tag="VGB16_01325"
     CDS             complement(2018..2731)
                     /locus_tag="VGB16_01325"
                     /inference="COORDINATES: protein motif:HMM:NF013760.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="MotA/TolQ/ExbB proton channel family protein"
                     /protein_id="HEX9916352.1"
                     /translation="MTFLYFLTIPLATPKPFSDNVIDMVAQSGLFSKIILLILLAFSI
                     SSWAIIFNKWRVFAKIGSETEKFLYLFHKKGRISEVYLNCTRLKDTPLSFVLGEGYKE
                     LQNFVGKEQNPLNQEKLDIIKMVLDKTGADELDKLETRTVFLATVANTSPFLGLLGTV
                     WGVMDSFASIGVKGTASLAVVAPGIAEALIATIFGLAVAIPAVIGYNHFNYRLKVVSS
                     EIDKFILEFMVKVKKEYSL"
     gene            2842..3024
                     /locus_tag="VGB16_01330"
     CDS             2842..3024
                     /locus_tag="VGB16_01330"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEX9916353.1"
                     /translation="MKRLLVILLIALFCFGLLAGCGPKKEEPKEGVTTEQPTTEQVAP
                     DTTQPPAADTTQPSGK"
     gene            complement(3359..3502)
                     /locus_tag="VGB16_01335"
     CDS             complement(3359..3502)
                     /locus_tag="VGB16_01335"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEX9916354.1"
                     /translation="MTEHKKGDTTSTPTQIAKRTTDEKWNYPTKITTTCQDNYTKDDK
                     NYS"
     gene            complement(3781..4134)
                     /locus_tag="VGB16_01340"
     CDS             complement(3781..4134)
                     /locus_tag="VGB16_01340"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEX9916355.1"
                     /translation="MNAWKVILATVLGFVCGIVCLLLAKSGGNPVAPAMAWAIILNRA
                     LIGFVIGISAWKIHFIPHGLLIGVFVSLSPGLAALATMGYNIFLGTLLMGAIYGLVIN
                     LVVSLVFQKKKEAAV"
     gene            complement(4311..4592)
                     /locus_tag="VGB16_01345"
     CDS             complement(4311..4592)
                     /locus_tag="VGB16_01345"
                     /inference="COORDINATES: protein motif:HMM:NF015925.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="antibiotic biosynthesis monooxygenase"
                     /protein_id="HEX9916356.1"
                     /translation="MVVEIFICYIKEENRQQALLDMAEYQHFKEKIPGHIRSYFKQSC
                     SEPHTFLVYSEFESKESYENYMQSFKEKTGGVPPLALLLEKPPIWGLFE"
BASE COUNT         1313 a         1084 c          917 g         1471 t
ORIGIN      
        1 taggtgacca ggtaggagag gaatctatgc ttgagctttt ggtcaagcgt cttatgatga
       61 caccatccgc attgaccaaa tagatttccg catcaccatc tttgcttaaa gtcatagcca
      121 aagttcttcc atctggagac caggccggag cggaattcaa ccccgcttgc gcggatatta
      181 ccttgctctt attatccttt aaactcaaga gccataaatc cggattccct cctttatagg
      241 aggtataagc gatggtcttt ccgtccggcg accaacgcgg ggaaaggtta attgacttat
      301 ggttggtaag ttgggtttgg ttttcaccat cataatcgca aacataaatc tccttgtatc
      361 cagtcctatc cgatatgaaa gctattctgc tgttgaagat tcctttttcc ccagtgagtt
      421 tgtaaacgat atcatccgag atggtatgag ccaaagcgcg ataactttcc acagaggtct
      481 tgtaggcctc tgaggatatt tttctcttgc gataaacatc ccataaatct acctcagcta
      541 tcacggttcc cttttttgct tccactgtcc cgccaactaa ggtcttcgct cccaagttga
      601 accagccagc aaagtcatac caatttcctt tcaggatgga agaggcgaaa gaatctgcct
      661 caaccagatt aaaaaccaaa gcgtaaagca agtcctgcct aactacagcg gttatatttt
      721 tcgacaaatc cttttcctgt gaaactgctt tgtcgttttt gggagtaaaa tcaatcactg
      781 ccatgtccat tggctcaatt ccagctgtgc ttatcttgac atgcacttcg gattgagagc
      841 aggatgtatc atacaggaaa acaacgagca ttagtggaat aatcgatatt agaatcttgt
      901 tagttaagtc ttttggtttc atagttattg cacaaattca aattccaaat gtactcccaa
      961 gtattctccg gtgtattcat ttggcagggg ctgcaggggg ctgactgaaa tgaccgccct
     1021 caatgcggct ttgtcaaaaa cccctacacc tgagctcttt tctatcttga catccactac
     1081 atccccatct ttaagtatcc tgaaatagac tatggcgttc agggtggaag aggcgctctt
     1141 tatcgggttt tcccacaggt cgtttatctt tgccacaaac atctgcacat agtaatcgcc
     1201 cccgaaatca gccccatcca cttgcgcgga gccaacaatt cctccggata tgcctgagga
     1261 agaaccagca gtagtgccac ccggcattct ttccttcttg ggttgctcct tgcttgaccc
     1321 tgttttattc tttcccaagt tttctttttt ggcattttgc tctttttccg aaatactttt
     1381 tttctcttta ctatttttca gctcttttat ggctatgcct ttttcactgg ctttttctgc
     1441 ctttcctgaa acttcagttt tcccaccctc tccgctccca ccagcaggtg caggcaaact
     1501 caccaagcca actctataga ttttgggata acctttcact ggttgaatat tcggaccaaa
     1561 aaggataatc gctattatca ccaacaagtg aaaggatatc gatattatta catctcgtct
     1621 cattttttct caaccggctg agtgactaaa cccaagtctt gtattcctaa actttttatt
     1681 ttgcctatga cctccaccac taaaccgtag ggaacatcct cgtctgcccg aagatatact
     1741 gttttggctc ttttcttcgc tttggcatcc aaaagtttct gctcaaatcg tcccatctgc
     1801 gtcaccacat catctatgaa aatcctcttc tccttggtga tggtgactac gattccctca
     1861 cctaaatctt cctgtccggc tttggtcttg ggaagattaa catctatgcc cgactgcagc
     1921 atcggtgcgg caatcatgaa gatgattaag agaaccaata ccacatctac cagattggtg
     1981 acattgatat cgtgcaatgc cgagtaggtt cttttcttca cagactatat tcctttttta
     2041 ctttgaccat aaattcgagg atgaatttgt caatctccga agatacgact ttgagtctgt
     2101 aattgaaatg gttgtatccg ataaccgccg gtatagccac tgccaaacca aagatcgtgg
     2161 caattagcgc ttcagcgatt ccgggcgcta ccacggctaa gctggccgtc ccttttacgc
     2221 cgatgctggc aaaggaatcc atcactcccc agactgtgcc taaaagcccc agaaacgggc
     2281 tggtattggc aactgtggcc aagaaaacag ttctcgtttc caatttatct aactcatccg
     2341 ccccggtttt atccagtacc atttttatga tatcgagttt ttcctggttt agtgggtttt
     2401 gctccttgcc cacaaagttc tgcaattctt tatacccttc ccctaagaca aatgaaagtg
     2461 gagtgtcttt caaacgggtg cagttcaaat agacttctga aattcttccc ttcttatgga
     2521 agaggtaaag aaatttctca gtttccgagc caattttagc aaaaactctc catttgttga
     2581 aaattattgc ccaggaggaa attgaaaacg caagaagaat taaaagtatt attttgctaa
     2641 ataaacctga ttgtgccacc atatctatca cattatcact gaaaggctta ggggtggcaa
     2701 gggggattgt taggaagtat aaaaacgtca ttttactttg acctcttttt tttaaaaaat
     2761 ctcttgacta aattcagttt gaacatatat ttctgcccat ataacttacg aaatctatcc
     2821 ttgaggaggt gatatagaaa gatgaagcgt ttactagtta ttctcctgat agctttgttc
     2881 tgctttggat tgcttgctgg ctgtggacct aagaaagaag aaccaaagga aggggtaacg
     2941 acggaacagc caaccaccga gcaagttgct ccggatacca cacaaccacc agcagcagac
     3001 acaactcagc catccggtaa ataataacat agattctttt tatctatggg gcagtgaaaa
     3061 ctcactgccc tttttttatt ttccattcaa agaacaaaag tattcaccca gccccaatat
     3121 atccaaatcg cattttggtg tcaagcaaaa ccaatttgtt tggagataat tcgaattcct
     3181 gtgtaacttt ccatccattc ctccagtata tagagtagag gcaagcttta ctaattatat
     3241 acgatgccaa actattatgt tccgaaaaag tgatggtcgg atttaaaaac agacaaacta
     3301 tgagggaaaa cgattttaaa ggagaaagat gaaaccccac agcttaacca ccttaatctt
     3361 aggaataatt tttgtcgtct ttggtataat tatcttggca agtagtggta atcttagtgg
     3421 gataattcca cttctcatcg gtggttcgct ttgctatctg ggttggcgtg gaggtcgtat
     3481 cgcccttctt gtgttcggtc atatctgcat tgtactcgga tgtttgctta ttactttggg
     3541 aatatatctt ttgccatact ccaaaccaac tttattacat gtttttacca gacccctttt
     3601 ttggggcttg ttttcattat ttggcggtat ttgtgcaaac taccatgggt tctgtcaatg
     3661 cattcatggt caaaagcaac ttattaagta attcattaca gcattgtttt tgtataaaac
     3721 aaagcgcggg caacttcttg cctgcgtttt tgctttcctg aaccttttaa attgcaaata
     3781 tcaaacagca gcttccttct tcttttggaa tactaacgaa acaacgagat tgatgaccaa
     3841 accgtaaatg gcacccataa gtaaagttcc taaaaagata ttgtaaccca tcgttgccag
     3901 agctgcaagg ccaggagaca aagaaacaaa gactcctatc agaagaccat gaggaataaa
     3961 atgaatcttc caagcactta ttccaatcac gaaaccgatc agggcacgat ttaaaatgat
     4021 tgcccaagcc atagcaggtg ccactggatt tccaccgctt tttgccaaaa gaaggcagac
     4081 tataccgcaa acaaatccca agaccgtggc gagaataact ttccaggcat tcatcttatg
     4141 ccctcctcgg tttaaggttt ttcctttaaa atattggatg tgatctggcg tatgagaaat
     4201 cttatataat ctgttgggct atggctcgga tgtcaaggga aatttaaatg aaggattgtt
     4261 cttcttgtgt atgggaaatt attctgattt ctttgggtaa gacaaaggag ttattcgaac
     4321 agtccccaaa tcggcggttt ttctaaaagc aatgctaaag ggggaacacc gccggttttc
     4381 tctttgaagg attgcatata gttctcgtaa ctttctttgg attcgaattc actatagacc
     4441 agaaaggtat ggggctcgga acaggattgc ttaaaatagc ttcttatatg tccgggaatc
     4501 ttctccttga agtgctgata ttccgccata tccagcaacg cctgttgtct attctcctct
     4561 tttatgtaac atataaaaat ctcaactacc actctgcctc cattttgtgg tggcgattag
     4621 acaatactcc acttgaggga ttttcccaaa acggcatccc cttattttat atgtcggatg
     4681 aaacggggaa aaagtttact aaaaatgcag cggaaagctt tagctttcca tatataaatg
     4741 gaggtctaaa gacctccgct atatctaaaa cgtaggtagg ggcgt
//
LOCUS       DASWLN010000038        47429 bp    DNA     linear   ENV 20-FEB-2024
DEFINITION  MAG TPA_asm: Candidatus Zixiibacteriota bacterium isolate
            SMAG_U2949 k141_674486, whole genome shotgun sequence.
ACCESSION   DASWLN010000038 DASWLN010000000
VERSION     DASWLN010000038.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37960594
            Sequence Read Archive: SRR10376091
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Candidatus Zixiibacteriota bacterium (soil metagenome)
  ORGANISM  Candidatus Zixiibacteriota bacterium
            Bacteria.
REFERENCE   1  (bases 1 to 47429)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 47429)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (26-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/12/2024 08:28:26
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 1,440
            CDSs (total)                      :: 1,416
            Genes (coding)                    :: 1,412
            CDSs (with protein)               :: 1,412
            Genes (RNA)                       :: 24
            rRNAs                             :: 1 (16S)
            partial rRNAs                     :: 1 (16S)
            tRNAs                             :: 21
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 4
            CDSs (without protein)            :: 4
            Pseudo Genes (ambiguous residues) :: 0 of 4
            Pseudo Genes (frameshifted)       :: 0 of 4
            Pseudo Genes (incomplete)         :: 3 of 4
            Pseudo Genes (internal stop)      :: 1 of 4
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..47429
                     /organism="Candidatus Zixiibacteriota bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_674486"
                     /isolate="SMAG_U2949"
                     /isolation_source="soil"
                     /db_xref="taxon:2053527"
                     /environmental_sample
                     /country="USA: Oregon"
                     /lat_lon="42.99 N 123.63 W"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            <1..455
                     /locus_tag="VGB16_01350"
     CDS             <1..455
                     /locus_tag="VGB16_01350"
                     /inference="COORDINATES: protein motif:HMM:NF013696.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016787 - hydrolase activity [Evidence
                     IEA]"
                     /codon_start=3
                     /transl_table=11
                     /product="M20/M25/M40 family metallo-hydrolase"
                     /protein_id="HEX9916357.1"
                     /translation="SARNIICDKVILKGTMRSLEKKVAGDLPIYVKQIASGIAQSHGA
                     DFKVAYNKGYPVLINDPKSTDTAREVTRNLYTKEAVYEIEKPVMGGEDFARFLEKIGG
                     TMMRLGIRNEKIGAIYPWHHPKFKIDEKALKFGSAVLAGIVLKSLQIK"
     gene            585..2078
                     /locus_tag="VGB16_01355"
     CDS             585..2078
                     /locus_tag="VGB16_01355"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEX9916358.1"
                     /translation="MRKIPLLLAFFLLFFHQVSFSYLYNEGDWTSYTMTRYVTSIAMD
                     DRTVYFGTSGGVIRYNKIERVWEDPFTTSDGLPDNRVNRIAYDKNEDVIWVDTPLGFA
                     SYNPTFKEWRREASFPLEWVRSDRDSLKLPDFFMEFGYDFFPDGYILDQRLSRYDITD
                     YLRDDWGDNLWLATWGLGVAFGSLRSWNLQLYTFGLYQNDVKDICIDGGVAWFGGNDI
                     FQNRVGITKYDRDKDKWEYFVEGYENNLGSARVNAIRASQNFVWFGTTQGLEVYSKKK
                     EEWSFYKRAKELRNSYITCLEYYRGSDKKDEGILYVGTYIGLCSYKVKKDSIAQFKDQ
                     NISSEYINCLKLSHNYLWVGTQMGTYRIPLAGGNAGVFSTPDGITNSNVNDIEEDKDK
                     VWFATDRGILGYNPITEEREVYQISGNYPGSQPLRLAIDGKNLWVGTKNGVWRMDKEK
                     KIWDNFTIFDGLIDDEIQAMVLDGDYIWFGTPKGATRLYWNNPRILR"
     gene            2081..3163
                     /gene="thiL"
                     /locus_tag="VGB16_01360"
     CDS             2081..3163
                     /gene="thiL"
                     /locus_tag="VGB16_01360"
                     /EC_number="2.7.4.16"
                     /inference="COORDINATES: protein motif:HMM:TIGR01379.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0009030 - thiamine-phosphate kinase
                     activity [Evidence IEA];
                     GO_process: GO:0009228 - thiamine biosynthetic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="thiamine-phosphate kinase"
                     /protein_id="HEX9916359.1"
                     /translation="MKLKQVGEFGLIDLIRKRVFSKGKEVVLGIGDDAAILKLNKSNL
                     QLLTTDALVEKIHFDLDYFPLEAVGWKGMVANLSDIAAMGGVPKAAVVTLGINNRFKV
                     EEILSLCSGISKAAKRFNCPIVGGDIVLSPRNLFLSIAILGEVKKGLAVRRIGARVGD
                     LIAVTGDLGSSSAGLEYLKLNKKPRSSSPSGSPLGARLSAKPREAEGSRGKPREAEGE
                     ATGFARKFLYPLPRLKEAQLITRFLKPKAMIDISDGLSSEAYHLTVENGLGANIYENN
                     LPISKIALKIAEGDKKKALHYAFNSGEEYELLFAFDGTRISQFRKLSRKIKISIIGKV
                     TRERKIRLTESDGKSRSLSYKGYTHF"
     gene            3581..5104
                     /locus_tag="VGB16_01365"
     CDS             3581..5104
                     /locus_tag="VGB16_01365"
                     /inference="COORDINATES: protein motif:HMM:NF024907.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="VCBS repeat-containing protein"
                     /protein_id="HEX9916360.1"
                     /translation="MLRSKLSYLLFAILFLCFGLNQLWADEGSIFTRQNLRVNGKIIG
                     KTYEDLNGDGLVDFLVFYLEGENENPNSPRGEATGRMVGLFEQQKGSGFDSIPNQTFE
                     LNKKASIVDLVDINKDNKKELLFLADDGIYYYSLNGDSFNKESSLLFSTNSFLPSSEE
                     NIVIWDFCPDFQNKGQLVIIPQKRGFEIWTRNDQGEFVRQDKLGFKPDISLSSSPGEL
                     NQKRGLIHFSYGIPGLIFTDYNKDGKEDILILKEDKIHVFLSKEDGFFGEQADKVLTL
                     KPKDNKEENIDFKIEDINGDGIIDLAVNQSGGDLEKGFKSKISIYLGKEGEGLNLDSP
                     HQVISSEKEQSSLELYDLNKDGKEEMIVSGAGFNIGSMIKILLAKSVKLDFDVRSLGK
                     NDIYPDQPNRKIKLNIKVSFGSSDEDNLAMDPSGDFNGDGLNDLFFAGGDNMLKFFLG
                     RKGDFFADKPQHELGIEIPAGNCKIIDLNNDNKSDIIFSFGDKKELRGKMVILFSKM"
     gene            5208..5399
                     /locus_tag="VGB16_01370"
     CDS             5208..5399
                     /locus_tag="VGB16_01370"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_013219106.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEX9916361.1"
                     /translation="MEHSFPECQKCGTGVLIPLSDYGREGATIRFKAWVCTNPECGFS
                     IRIDNGEVSYGKTIGHSTK"
     gene            5466..6389
                     /locus_tag="VGB16_01375"
     CDS             5466..6389
                     /locus_tag="VGB16_01375"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEX9916362.1"
                     /translation="MEKIFSNLGLKIAALFLALLLWFHATTDKTFIYDFDLPLVISNM
                     PSDLILASPVPSKIEVKIRGKGKQLIKYFLSDKEDVVIDVSQLKYREMDYVIRPEEIP
                     LPKDLSLEVEKVDSPKQIKILLDYFSEKKVPIRNQMEVITQSGYVQIDSLGLKPDKVL
                     LSGPRTLTRKIDQVYTERKILGSLNSSTSGLVPLIVPEGFNLKLSPDEVEYKVLVEKL
                     TQKEFTQISVELVNLPVKKKVKIEPKSIDLFVQGVSQKVEQITPDKIKVSLDCDRLGT
                     RKEGKIVPQVKLPKEVELVRIVPDSIKFTVE"
     gene            6453..7457
                     /gene="tsaD"
                     /locus_tag="VGB16_01380"
     CDS             6453..7457
                     /gene="tsaD"
                     /locus_tag="VGB16_01380"
                     /EC_number="2.3.1.234"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_018999875.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_process: GO:0006400 - tRNA modification [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="tRNA
                     (adenosine(37)-N6)-threonylcarbamoyltransferase complex
                     transferase subunit TsaD"
                     /protein_id="HEX9916363.1"
                     /translation="MLILGIETSCDETSAAITRDGRILSNVIYSQLIHTRYGGVVPEI
                     ASRQHIKTIMPVVQEALSRAKTPLKKIEGIAVTYGPGLVGCLLVGISWSKALAYSLKI
                     PLIGINHIEAHIFANFLEHPKPMPPFVCLVVSGGHTLLVDVKEKGNYRVMGQTRDDAA
                     GEAFDKVAKLLEIGYPGGPLIDRISKKGKRDFVRFPRAYLEKDSLDFSFSGLKTAVAI
                     YASGLSKTKINRNKKDIAASFQEAVADVLIEKSIRALRVAKSKRLGLAGGVARNSRLR
                     EKLEAKAKDLKFDLYYPSYELCTDNAAMVAACGDFYLSQGIKSDLSLNAIPYAKISSI
                     "
     gene            8118..9272
                     /locus_tag="VGB16_01385"
     CDS             8118..9272
                     /locus_tag="VGB16_01385"
                     /inference="COORDINATES: protein motif:HMM:NF013188.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_process: GO:0006812 - monoatomic cation transport
                     [Evidence IEA];
                     GO_process: GO:0055085 - transmembrane transport [Evidence
                     IEA];
                     GO_process: GO:1902600 - proton transmembrane transport
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="cation:proton antiporter"
                     /protein_id="HEX9916364.1"
                     /translation="MTKILLDLLIMFAAAKLLGEVFERFKLPAVIGEILAGVCLGPYV
                     LHIITPTEFYHVLAEIGVIILLFYVGLQTGIDEMIKVGKNSISVAILGVVLPFILGYA
                     YILFSGHSAVEGMFIGAAMVATSVGITARVMADLNVLDKKVSKVILAAAVADDIMGMI
                     VLAIVTGMGKGTLSYLNIILVILEAVVFVIFLMVIGHRVIRRVTPGVDSLKTRNAPFA
                     FAIILCLALSVLAGYIKLAAIVGAFMAGMVLSEFNIRFRLTLKMESLYDFLVPFFFVV
                     MGANVDLTVFGKWSILGLALILTFLAIIGKLIGCSLGAMNLGFRQATIIGVGMVPRGE
                     VGMIVASIGLSLETISRDLYSVVIFMVVMTTVLTPPILRMLLSRWRGFLS"
     gene            10331..12172
                     /locus_tag="VGB16_01390"
     CDS             10331..12172
                     /locus_tag="VGB16_01390"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEX9916365.1"
                     /translation="MRFTSKLLIISILFILFVIPCGVAYSQDPTIWYVTFDPGIGDYT
                     LPKPNSISAFIWFPEIADMDTTIYYPLTFTLDGLLIYNWEPNSEFVQSGYNVTSNKYF
                     HRPEYASYDVLDSIRLDIELTDSLSLGAHEWKVELWLVDWPFEPWFAGAKTFYADTVW
                     YAPSEATSLALIPEKDHYTCNSPKFEAAFSSLPGYQLIDTTVKFTLYAPNETLDVYDG
                     SRDTAVLDGYYARSDVAQLKLFIEVDDEQTLLDSSQYSYEISAIAVSLTLPRIIYSKE
                     DTFSVGNLTTPKEIIPQSFSYVGPRPEFYYTIGRSGTALDTTNIYLDLFYVTFDYDTV
                     GDSTIEKRNYLKTIYPNQMSKFATDTVKVATNLELIDMAAMDVLVCGQADQYARQGYE
                     NFVPYTLRMGYMDTVSNKRGPAYKRFIVDRVSPQITMLDSTDKAWKFHIQDGGSGVNN
                     SSVVVTQNGQKVTNDALVQYNPTTHILTYYPLTLDALFTLEAEDNVGNEGSYSTYVKS
                     GRLIAYDMHSYPNPFNPTKGEEATIVLSSNRPDNQALEITARVCDLAGKHVATLKQNA
                     GKLLWNGKTDGGELVANGVYLCYVSIRDLNDLTVQNYLLKIAVVKKD"
     gene            12203..14206
                     /locus_tag="VGB16_01395"
     CDS             12203..14206
                     /locus_tag="VGB16_01395"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEX9916366.1"
                     /translation="MRKLSKSVMLIGVIFLIFGWSGVSFAEYEVFFVPSFQDSFTTTR
                     PEIRIKFPKGGVGPTKVAIDTLKRCANEPYYGFIDSTGRFPRLDSIYYTKTSPADTIV
                     RAGFGDSMYQTDGYNVLCLFWDTATALPARDSASGKPAYALKVDSAGTVFRYPLTPCK
                     YNVSRAGELVVKPAHKSNDYTNRTPIFQAEFSIFKEVKGDTTWYDGVFLDTNLTVVLK
                     ATGITDTLYDYKRFPQLRPGLIGNLRKIHPGKMVFTISWLDTSYYLPLDMTKAYIDSV
                     TGGVWDYQGLSIICGSPPLGYSPRGVADTFWVDGTPPEMELQEMNYVSKSPYFLLKAS
                     DAKAGVNTDSLFVDLYKVELYGEAPPDDSVEDRSYLKSLQVEGLDPSLGSVLVNTGVE
                     LEDVEGGMALDVWVFNGRPFDVNEYMTNLDAVYPDSLGPMDGAGNLITPLYKRFYVDG
                     IPANVRLSSNSTHEIRIFEISDTSSCYQVDTLGHVEILCSGVNTASVKLYENFQLVTP
                     DSTKYNSSSGAYSVYYSPTSENVWINLDVADKVGNIATYQNYYEDDQLNLVEPHNYPN
                     PFSPVSPIVDHRRTVIDPGLTRTTDMELTIDIYDLAGHHVVSVTPNMADGGRGYWDGR
                     TKDGTLVANGVYLCYIQARDTAQNKTLTEVIKIAVAKNDKRIF"
     gene            14233..15150
                     /locus_tag="VGB16_01400"
     CDS             14233..15150
                     /locus_tag="VGB16_01400"
                     /inference="COORDINATES: protein motif:HMM:NF033709.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="PorV/PorQ family protein"
                     /protein_id="HEX9916367.1"
                     /translation="MSNFIRNIVLLAIVFSMLCVPPLLADDNHAMPVLRMGVGARALG
                     MGGAYIAEVKDASATYWNPAGLTEMEHLNITGMFSSEMSYERNHNFLAYGRKFGFGAL
                     AMGWISHEVRGVHGYSYENLPTSTFREVDNVFMFSYARKIGKFSSGANFKIVDQRMGD
                     YLSETGIGFDLGVRYSYNDDLVFGAVVKDIATKVGGWTGKYEVWKDRVPANFGVGAVV
                     YPYAGFTFPVDVYKVEGREDMALHFGGEYTYKLEEKYSFSLRAGSNDGQIAFGFGLKF
                     VKFTIDYAFMNEQQDFLKENHKFSFSADF"
     gene            15626..16108
                     /locus_tag="VGB16_01405"
     CDS             15626..16108
                     /locus_tag="VGB16_01405"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_015207348.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="adenosine-specific kinase"
                     /protein_id="HEX9916368.1"
                     /translation="MKTEQYKIQVPEGANLILGQTHFIKTAEDLYEILVSSVPNIKFG
                     LAFCEASGPCLVRHEGNDEKLRSLAKKTAFEIGAGHIFVIFIEDAYPINVLRGIKECA
                     EVCSIFCATANPVELLIAQTEQGRGVIGVVDGFSPKGIEEDKDIKERKQFLRKIGYKL
                     "
     gene            complement(16110..17888)
                     /locus_tag="VGB16_01410"
     CDS             complement(16110..17888)
                     /locus_tag="VGB16_01410"
                     /inference="COORDINATES: protein motif:HMM:TIGR04183.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="T9SS type A sorting domain-containing protein"
                     /protein_id="HEX9916369.1"
                     /translation="MKKTILLLLIYFAFLDTAFSQSPTDPTFHKYDTSNFPVFDNCFE
                     LYVTDGVVQNWEDKYTSDFINLILDDDTTNNSDIFPVVWNYFNPNDLDSSQAGIRFTI
                     WDSTYTKIKYYLYDPNDAQDCNGSNPSYFCKGYGWYAGRNVFYSAIRVRYGTGLLYPG
                     DPLIMGLFAHELQHLCNQGNTNYDNEWVNETKSELSRFLTGYKKVNIGVFNYNIPFDN
                     PLPWFSSGPPLTSKYWHWYLWGVYLYEQFPGLTDSIHDDLWYQYIRRESGTFSGMYSL
                     ARMFEKDEFKRYGLSGDDLLREVFTNWAMANLLDDTTIEDGKYGYKNVEVKNEIGYLK
                     INYGNPDSLQNCGYAPALHLVDTSFINNEKTYSVIYGKDSLFSYLYSSDYIELSADAS
                     LDNGKTYQLDFRLQKDQIDPELNTILLGKIVTYDEKGGVISVERLSNFPFGISVSISD
                     FGKNIQEAILTVVAVEDQIKEGYYYVQDGKQYYTYSIELKEHTGEDPNNPPKGPKLEQ
                     NYPNPFNSSTTISFYLDKQAEVNITIYNVLGQKVFTLNQNMLSGQNRIEWNAKDNSGM
                     DLPSGIYFYNLKTKEFSETKKLIFLR"
     gene            complement(18118..18525)
                     /locus_tag="VGB16_01415"
     CDS             complement(18118..18525)
                     /locus_tag="VGB16_01415"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_012696971.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="single-stranded DNA-binding protein"
                     /protein_id="HEX9916370.1"
                     /translation="MASVNRAILIGNLGRDPDLRYTPSGQPVAKFPIATSERWKDKDG
                     QLQQRTDWHNIVAWGRQAEVCKEYLKKGSPVYIEGRIQSRSYEDKDGNRKYITEIVAN
                     RMQMLGRKEEGPSEEEIPPEPETEEAEGDDIPF"
     gene            18682..19650
                     /locus_tag="VGB16_01420"
     CDS             18682..19650
                     /locus_tag="VGB16_01420"
                     /inference="COORDINATES: protein motif:HMM:NF012745.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016757 - glycosyltransferase activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="glycosyltransferase family 2 protein"
                     /protein_id="HEX9916371.1"
                     /translation="MNNHPQLPSASPRGGRRETSPQVSIIVPAYNEAENIPLLMEKFS
                     RMFQSSGLKGEVILVNDGSTDNTLAQAKTCQEKYGFLRVASHKVRKGITDALLTGFSD
                     SQGEVFVFWPADLQYLPEDIPHMIEKIDQGFDIVTGWKQGNYGLKGFVSFCYNLLSRI
                     LFGIRVHDLNSVKAFRRDIFEDVTLRKDWHRYMVVLAAEKGYKIAEVRVKLYPRKYGK
                     SKFGFWRIPIGILDLFSVKFQLSFLRKPMLFFGFLGLVLFFLGVVVAGLALYLRFIEH
                     EGFRPLLYLVILLVLSGISFFALGFLAEAIVSLREELRDIKNKLIK"
     gene            19973..20686
                     /locus_tag="VGB16_01425"
     CDS             19973..20686
                     /locus_tag="VGB16_01425"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_018197755.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="polyprenol monophosphomannose synthase"
                     /protein_id="HEX9916372.1"
                     /translation="MKTLVIIPTYNESTNIATLLPQILAQDPTIDVLVVDDNSPDGTG
                     KLVDKMTGENSRIQVIHRQKKSGLGTAYKAGFKHALERGYDYIFEMDADFSHDPGYIP
                     HFLEAIKEADLVLGSRYIRGVNVINWPMSRLLLSYFANIYTRWVTGLPIRDSTGGFKC
                     FKREVLESINLDRVKSNGYTFQIEMSFRAWKKGFRLKEIPIIFEDRREGQSKMSKKIV
                     REAVWMVWKLRLLSILGKI"
     gene            20638..21582
                     /locus_tag="VGB16_01430"
     CDS             20638..21582
                     /locus_tag="VGB16_01430"
                     /EC_number="2.4.-.-"
                     /inference="COORDINATES: protein motif:HMM:NF025025.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016757 - glycosyltransferase activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="glycosyltransferase family 2 protein"
                     /protein_id="HEX9916373.1"
                     /translation="MDGMEVEASEYSGQDMTLSFDVSIIIVNYNVNKLLFKCLSSVFR
                     TNSKKKMEVIVVDNASRDKSTETVKKDFPQVKLIQNRKNLGFARGANQGIKASRGRYI
                     FLLNPDTLITAGELDKIIDFMEKNKKVGICGPKMVNSQENIQYSCRKFPNYLTSISSS
                     QSLLFKLFPRNPLSQEYLLTEKSHREEMEVDWVSGSALLARKDMLDQIGNLDENFFIY
                     VEDVDLCYRAKKGDWKVFYYPYAEIRHLIGASTNQERSKMVIQHHKSMYHFYQKHYSA
                     KAILSILVLWGIFLRMGIVLLSQMVELILQTNSKKEKV"
     gene            21579..22628
                     /locus_tag="VGB16_01435"
     CDS             21579..22628
                     /locus_tag="VGB16_01435"
                     /EC_number="2.7.8.-"
                     /inference="COORDINATES: protein motif:HMM:NF013146.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0046872 - metal ion binding [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="MraY family glycosyltransferase"
                     /protein_id="HEX9916374.1"
                     /translation="MIVLFTFIISLLISFAITFWIRNVCIRHNLLDHPDQRKMHTTPI
                     PRLGGVGIFLSILVSLLIVFLFKSSLFREIESQILFVLLSGMVIFGLGLYDDLKPVKP
                     EIKLIFQAIASLILILAGLRINLIHIPFYRTINLGWFSYPLTLIWFLVIVNAMNLLDG
                     LDGLAAGVSVIAAGSLLVVGYMLKVDIALLISASVAGSCLGFLRYNYFPASIFMGDSG
                     SLFLGYLFALMAIICPIKSYTSMALFVPLLALGVPLIEITISSFRRIIGKQTLYLADK
                     RHIFHFLIEKGFSHRDTVWLFYLLSLIFAIMVISLLTQSHKIALLLVFLLILTFGTIF
                     VSWYNRTQAKLKAEK"
     gene            22634..23608
                     /locus_tag="VGB16_01440"
     CDS             22634..23608
                     /locus_tag="VGB16_01440"
                     /EC_number="6.4.1.2"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_009184701.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0009317 - acetyl-CoA carboxylase complex
                     [Evidence IEA];
                     GO_function: GO:0003989 - acetyl-CoA carboxylase activity
                     [Evidence IEA];
                     GO_process: GO:0006633 - fatty acid biosynthetic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="acetyl-CoA carboxylase carboxyltransferase
                     subunit alpha"
                     /protein_id="HEX9916375.1"
                     /translation="MNGIHLEFEKPIYELEKKIQDMKDFADGENVELTQEIELLEKKL
                     DKLKRETYSNLSRWQRVQLARHPKRPHSLDYINLMLTDFVELHGDRNFADDKAVVGGV
                     AKLEGIPLLVVGQQKGKDTKENLFRNFGMCNPEGYRKGLRLMQLAARFGRPIVILIDT
                     PGAFPGIGAEERGQAEAIARNLREMARLPVPIIVNIIGEGASGGALGFGIGDKILMLE
                     NSWYSVISPEGCAAILWRDSSKAPQAAEALKLTAWDLEELGVIDKIIEEPLGGAHRNY
                     EESARILKKEIKAVLEELSNLGPEELIERRIAKFRQMGSYGIEKEEET"
     gene            23613..23777
                     /locus_tag="VGB16_01445"
     CDS             23613..23777
                     /locus_tag="VGB16_01445"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011288933.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="Trm112 family protein"
                     /protein_id="HEX9916376.1"
                     /translation="MLDWNSLSILVCPKCKGQLKHRHKPEGLVCQACRLDFEIREDIP
                     ILLLEEARKF"
     gene            23803..24255
                     /locus_tag="VGB16_01450"
     CDS             23803..24255
                     /locus_tag="VGB16_01450"
                     /inference="COORDINATES: protein motif:HMM:NF012579.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:1902495 - transmembrane transporter
                     complex [Evidence IEA];
                     GO_function: GO:0016301 - kinase activity [Evidence IEA];
                     GO_process: GO:0008643 - carbohydrate transport [Evidence
                     IEA];
                     GO_process: GO:0009401 - phosphoenolpyruvate-dependent
                     sugar phosphotransferase system [Evidence IEA];
                     GO_process: GO:0016310 - phosphorylation [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="PTS sugar transporter subunit IIA"
                     /protein_id="HEX9916377.1"
                     /translation="MKLSKFCEENLISFELKAKDKEGAIRELADLASRSSLVKDKEEL
                     HKAVIEREKLVTTGVGYGVAFPHAKTKAVKGIVIAFGRSKQGIDFEAMDKKPVYLFFL
                     IAAPEDVIGAHLNVMARLSYLMKGEKNRDTLMKIKSPRELIEILDSVD"
     gene            24258..25049
                     /gene="mreC"
                     /locus_tag="VGB16_01455"
     CDS             24258..25049
                     /gene="mreC"
                     /locus_tag="VGB16_01455"
                     /inference="COORDINATES: protein motif:HMM:TIGR00219.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_process: GO:0008360 - regulation of cell shape
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="rod shape-determining protein MreC"
                     /protein_id="HEX9916378.1"
                     /translation="MPKFAPITLKGNKYFYLSILILLSLILLIMPHYTKTIISYYVFK
                     FSYSPFYGLSDKVGELRGVYKKNQELEKRVAELTLENYQLKEQSIENATFRALLKFKS
                     ESSYKVIPAKVIAFDPGRNTSTVLVDVGGEEGIRRNMPVINMYGLVGKTLEVMPHTSA
                     IQLLSDPNCRVAAIDQRSRILGIVKLGPGLALVMDNVPFGEDIKAGDQIVTSGLGGIF
                     PSGIQVGKVAEVEEAKHAVFKTIHLKPEVNFNSLEELFVLDVSQG"
     gene            25053..25571
                     /gene="mreD"
                     /locus_tag="VGB16_01460"
     CDS             25053..25571
                     /gene="mreD"
                     /locus_tag="VGB16_01460"
                     /inference="COORDINATES: protein motif:HMM:NF016019.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003674 - molecular_function [Evidence
                     IEA];
                     GO_process: GO:0008360 - regulation of cell shape
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="rod shape-determining protein MreD"
                     /protein_id="HEX9916379.1"
                     /translation="MFFKNLRYSKAVDLVIFLLMMLIYQSIISRGVEVAGITLDFALI
                     ILVYVGLTRGAVFGAVFGFASGLLLDVYTPGLLGLGALLRGILGFLIGNFRNKLFIET
                     LYSKGGMIFIALLWHDLFYYLFATTFQIGATTYILLFNSLPSGLYTAVIGMVVFGLVQ
                     KRLTQPGQVTGL"
     gene            25568..27403
                     /gene="mrdA"
                     /locus_tag="VGB16_01465"
     CDS             25568..27403
                     /gene="mrdA"
                     /locus_tag="VGB16_01465"
                     /EC_number="3.4.16.4"
                     /inference="COORDINATES: protein motif:HMM:TIGR03423.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003824 - catalytic activity [Evidence
                     IEA];
                     GO_process: GO:0009252 - peptidoglycan biosynthetic
                     process [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="penicillin-binding protein 2"
                     /protein_id="HEX9916380.1"
                     /translation="MKDATYPREKRSAILISILAILLSLLIIRVFGLEVIRGTYYRQM
                     SEENRIRPVPLPAPRGLIVDRNGKVIVSNRASYTVSIIPYEVRNLKEVVGRLSPLLQI
                     DADGLMQRIKDNWSRRFEPITIKRDVDFTTISILEEQNQDFPGVIYQVVQTRKYPEIR
                     SISHLIGYVSEISSQELASLPAQRYRVGSYIGKKGIEKQYDDLLRGKDGMLYLEVSAI
                     GKILGELKDKEPDPPVAGSDIKLSINFDLQKLADSLLEKHSGATLVALDPNSGEILAL
                     VSKPGFDPNIFAGVLTPEEWNSLSQDPNHPLLDRAIQGTYPPGSTLKLLTAGAALETK
                     VIQQENSFASCVGGYRFGNRIFKCWQERGHGKLKLHEAMVQSCDVYFYQLGLKVGLEN
                     WCHYAGLCGFGEKTGIDLPEEAKGFAPTLSYFIKRHGKGEWVKGLVINLAIGQGEISA
                     TPLQMATFYAGLANKGIIFKPHLLKQAITPSGKVFVTKTEQLIKLPFSPETLDTLNQV
                     LIGVVNEPFGTGGASRLADVTVAGKTGTAQNPFGGDHAWFICYAPAEAPQIVLALLVE
                     NAGHGGTIAAPIAKRILQAFFEKNKELHPQGGVLNPNRDPESSSG"
     gene            27579..28805
                     /gene="rodA"
                     /locus_tag="VGB16_01470"
     CDS             27579..28805
                     /gene="rodA"
                     /locus_tag="VGB16_01470"
                     /inference="COORDINATES: protein motif:HMM:TIGR02210.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_process: GO:0008360 - regulation of cell shape
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="rod shape-determining protein RodA"
                     /protein_id="HEX9916381.1"
                     /translation="MKLKFPQENFDIWLLVTALSLSLIGLLLVYSASRNVSLPAGSHL
                     FSKQVVWLLLGLVCGAVFFVIPLKLHDFFSGFYYLIGIGLLLILDFVGNEKGGAIRWF
                     NFGSFAFQPSEFVKLALVFILARYLAYSQRSFLNFRWLFTIFAITLVPMVLVLKQPDL
                     ATSLIFIATLVVMLFWAGVSPLYILLLLSPFISLVAAFHWITWGIFFFFVLLLLYHLR
                     PNFLLSSSLILFNLLFGMVTPLIWNRLHDYQKLRILAFLDPTSDPQGSGYQIIQSKVA
                     IGSGGFFGKGFLHGSQTKLAFLPAQHTDFIFSVLGEEFGFWGVLALLALFFVLLLKGF
                     VIARKARNSFASFVALGISSVLAFQVVVNIGMATGIMPVAGLPLPFISYGGSSMLLAW
                     ICTGILLNINYHWYEY"
     gene            28821..29618
                     /locus_tag="VGB16_01475"
     CDS             28821..29618
                     /locus_tag="VGB16_01475"
                     /EC_number="2.5.1.145"
                     /inference="COORDINATES: protein motif:HMM:NF013915.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0016020 - membrane [Evidence IEA];
                     GO_function: GO:0008961 -
                     phosphatidylglycerol-prolipoprotein diacylglyceryl
                     transferase activity [Evidence IEA];
                     GO_process: GO:0009249 - protein lipoylation [Evidence
                     IEA];
                     GO_process: GO:0042158 - lipoprotein biosynthetic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="prolipoprotein diacylglyceryl transferase"
                     /protein_id="HEX9916382.1"
                     /translation="MHPVLFRLGPLAITSYSLLLVLAFVLGVWLAIRRARKSGVKVET
                     IVDLAIVIWISSIVGSRFFYVIFHLEEFKGRFWDVVNPIHPGGDIGIVGLSMVGGVVL
                     AILTGLVYLYLKRLEVWKIADIVSPSFILGLAIARLGCFLSGCCFGKPTGSFLGVVFP
                     LDSPAGYVFPDAHLLPTQILSSIGAVIVLVILLFLERYKNFEGFTFWLMVMLYSINRF
                     VIDFFRYYEPSTTFKVLHTAFSSNQITLFFAFFVSLFMFVWFEKRAR"
     gene            29795..30361
                     /locus_tag="VGB16_01480"
     CDS             29795..30361
                     /locus_tag="VGB16_01480"
                     /inference="COORDINATES: protein motif:HMM:NF012383.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ComF family protein"
                     /protein_id="HEX9916383.1"
                     /translation="MECRSLLEKDKECKQCGDKDGLSLVYSLGSFDDSYQRLIYAFKY
                     GQLLNLGKRLGSTLGEKIKEDKRFLGCDFLIPVPLHPSRKRKRGFNQSEILALAISEK
                     IGLPVLKDVLKRKKRTKDQTTLNAKQREENVRGAFSVKNEDRIVDKQIILVDDVMTTG
                     ATLKECARNLFEAGAKEVLGLTVAVAGE"
     gene            complement(30364..31176)
                     /gene="mutM"
                     /locus_tag="VGB16_01485"
     CDS             complement(30364..31176)
                     /gene="mutM"
                     /locus_tag="VGB16_01485"
                     /EC_number="3.2.2.23"
                     /EC_number="4.2.99.18"
                     /inference="COORDINATES: protein motif:HMM:NF002211.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003676 - nucleic acid binding [Evidence
                     IEA];
                     GO_function: GO:0003684 - damaged DNA binding [Evidence
                     IEA];
                     GO_function: GO:0003906 - DNA-(apurinic or apyrimidinic
                     site) endonuclease activity [Evidence IEA];
                     GO_function: GO:0008270 - zinc ion binding [Evidence IEA];
                     GO_function: GO:0008534 - oxidized purine nucleobase
                     lesion DNA N-glycosylase activity [Evidence IEA];
                     GO_function: GO:0016799 - hydrolase activity, hydrolyzing
                     N-glycosyl compounds [Evidence IEA];
                     GO_function: GO:0019104 - DNA N-glycosylase activity
                     [Evidence IEA];
                     GO_process: GO:0006281 - DNA repair [Evidence IEA];
                     GO_process: GO:0006284 - base-excision repair [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="bifunctional DNA-formamidopyrimidine
                     glycosylase/DNA-(apurinic or apyrimidinic site) lyase"
                     /protein_id="HEX9916384.1"
                     /translation="MPELPEVETIVRGLRKTILGKRIKEVKFLCPGIVKQSSVSFICQ
                     TVGQKVKDIKRRGKNIVICLSQDNAILVHLGMSGHLLYLCPDRKVDKHDHIIFRFLGD
                     VKELRYNDPRKFGKMRFVDLKTCPDLDKLGPEPMEISEKEFVDLMRKRKGRVKSVLLN
                     QKVLAGIGNIYADEVLFEAGINPLKKIDQINTVKLRGLRKSIGKILAKAMRTGGSSIR
                     TYTDLSRKMGRFQLSHKVYGREGKPCKRCGVKIKRIVINQRSSYFCPRCQKP"
     gene            complement(31491..32402)
                     /gene="nadA"
                     /locus_tag="VGB16_01490"
     CDS             complement(31491..32402)
                     /gene="nadA"
                     /locus_tag="VGB16_01490"
                     /EC_number="2.5.1.72"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_013217622.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008987 - quinolinate synthetase A
                     activity [Evidence IEA];
                     GO_function: GO:0051539 - 4 iron, 4 sulfur cluster binding
                     [Evidence IEA];
                     GO_process: GO:0009435 - NAD biosynthetic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="quinolinate synthase NadA"
                     /protein_id="HEX9916385.1"
                     /translation="MENNNITKRILELKEKRKAIILAHNYQLGEVQDIADYVGDSLGL
                     SQTASKTEAEVIIFCGVHFMAETASLLCPNKLVLMPDLNAGCAMANMITLRQLRETKR
                     RHPHAVVICYINSTAQIKAESDYCCTSANAVKIVEHIPKDREVLFIPDKYLGSYVASQ
                     LKRDLILWEGYCPTHRRILAEDILKNKNLHPKAEVLVHPECTPDVIAMADKVLSTSGI
                     IRYVSESSSEEFIIGTEIGILHALKKKEPNKKFYPASLLADCSNMKLTNLEKILWSLE
                     DMLYEIKVPQDIAAKARKSIERMLELS"
     gene            complement(32418..32627)
                     /locus_tag="VGB16_01495"
     CDS             complement(32418..32627)
                     /locus_tag="VGB16_01495"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011393973.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF1858 domain-containing protein"
                     /protein_id="HEX9916386.1"
                     /translation="MEQKLIKEMTFGEVLSKYPKTAETFFRYGMHCFGCHIAAEESIE
                     QGALAHGVDVDKLMDDLNKTVSEEK"
     gene            complement(32806..34812)
                     /locus_tag="VGB16_01500"
     CDS             complement(32806..34812)
                     /locus_tag="VGB16_01500"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011344237.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="heterodisulfide reductase-related iron-sulfur
                     binding cluster"
                     /protein_id="HEX9916387.1"
                     /translation="MEITREIFWNVGAGIRIIIYILAIIPLLFLIWGIYKKIKLWRIG
                     KPEKKFDKIGKRILSFVSFGFFHQRFFRDLYPGLMHFFIFWGFVVLFLGTAVVMFQDD
                     IASPLFGIFFFHSYFYLYFSLILDLFGLLLIIGIGMALFRRYILKPKRLDNILDDAVV
                     LILLLAIATGGFLNEALRISATKPQFEVWSFVGWNLSKLFWNLNPNSIRILHLISWWI
                     HLALALFFISYITYSKLLHILSSSLNIFFRSFEPKGRIPAILDIENQETFGVDRVEQF
                     NWKQLLDTDACTRCGRCQDNCPAYLSQKPLSPKKVNQDIKANLQARTRELLGKDKKGT
                     PSVALIGGSVKEDEIWSCTTCYACHQACPVLIEHIDKIVDMRRHLVLMQSRFPNELKN
                     LFKNMETNYNPWAFGYQARADWAKDLGIKLAREDKNFDILYWVGCSGSFDDRNKKVSR
                     ALVKILKTAKVNFAILGNEEMCCGETARRIGNEYLAQILMRGNIEILKKYGVRRILVT
                     CPHGYNAFKNEYREFGGDFEVIHHTELIWNLIKDNKIKLKSQNEKSITYHDSCYLGRY
                     NGIYDAPREIITRLSQKKLNEVKLSKSKSFCCGAGGGRMWMEENLGKRINQMRIEQLN
                     STGSEVISTACPYCLTMLEDGVKEKTLEEKLCVKDIAELVEELI"
     gene            complement(34821..35819)
                     /locus_tag="VGB16_01505"
     CDS             complement(34821..35819)
                     /locus_tag="VGB16_01505"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_015311837.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0009055 - electron transfer activity
                     [Evidence IEA];
                     GO_function: GO:0050660 - flavin adenine dinucleotide
                     binding [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="electron transfer flavoprotein subunit
                     alpha/FixB family protein"
                     /protein_id="HEX9916388.1"
                     /translation="MANEVWVIAEQRDGKLKKVSFEILSCARKIADKLKVQLGAILLG
                     DKVECLANVLGYYGTDKVFLCDSEILKNYSNEGYSQVIADFVKEHKPKILLGPSTAMG
                     KDLLPRVAAKLGTGLASDCITLDIDSNGNLLLKRPIYAGKVIVDVFIDSSPQMASIRP
                     NVLVMASPDESRRTEIIKSEVRISTEGIRAKVKEIVKSAGEKVDLTEAKVIVSGGRAM
                     KGSENFKILEELACSFGEGATVGASRAAVDSGFAPHDMQVGQTGKVVNPEVYIACGIS
                     GAIQHLAGMQTSKCIIAINKDPEAPIFQKANYGIVGDLFQIVPLLAQEIKKTKAGN"
     gene            complement(35943..36728)
                     /locus_tag="VGB16_01510"
     CDS             complement(35943..36728)
                     /locus_tag="VGB16_01510"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_006419660.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="electron transfer flavoprotein subunit beta/FixA
                     family protein"
                     /protein_id="HEX9916389.1"
                     /translation="MNIVVCLKQVPDTETKIQINQGKDGISEEGVKFVINPYDEYAIE
                     ESLRIKEKFGGEVTILCLGPERVTESIRTALAMGADKAVHLCDPAFEKLDSYSTAKAL
                     SMALKNIPFDIILTGKQGVDYDNSQVFSVLAEILNIPQVSVVVGLEISDDKTKAIAKR
                     EIEAGGIEIVETSLPVIIAAQKGLNEPRYASLPGIMKAKKKEIKNLTLSDLGIPVDEL
                     RNKIKLINFGFPPPREAGKIIQGEAIEVAVELAKILHQEEKII"
     gene            36951..39113
                     /locus_tag="VGB16_01515"
     CDS             36951..39113
                     /locus_tag="VGB16_01515"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003253305.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005524 - ATP binding [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="UvrD-helicase domain-containing protein"
                     /protein_id="HEX9916390.1"
                     /translation="MAKILEELNPPQKEAVLHTEGPLLVLAGAGSGKTRVLTYRIAYL
                     VGEKKVNPYNILAITFTNKAAREMKSRVDMLLGRGSDFLWVSTFHSLCSRILRKECQV
                     LEYKKHFSIYDEADQISLIKKCEEELGIPIKEFSPAAVLNRISQAKNNLIGWQDFASI
                     AQNHFEQKVSKIYPLYQKKLKENDAFDFDDLIMKTVEIFENHPQVLENYQDRFKYILV
                     DEYQDTNHAQYALIKHLSSKYKNLCVVGDDDQSIYAWRGADLNNILDFEKDFPDTKVV
                     RLEQNYRSTQIILDAAISVVKNNVSRKGKVLWTEKKGGEKLVVSYLEDDRMEAKGVVQ
                     RMDHLVANFGYGRSDFVVLYRTNAQSRIIEQELRDKGIPYTIVGGVKFYERKEVKDVL
                     AYLRVLVNADDQLSLKRIINVPHRGIGQKTMARIEEEAFKKRKNLLETLKHIRDVQGI
                     SDRMKKEINSFANLLNELNQLKKSLKVDQLTEEVIQRSGYLDRLKEERTIEAQTRAEN
                     VEELVAATKEFVERVENPSVESFMEEVSLLTDIDQWDDTQDVITLMTLHAAKGLEFPA
                     VFITGLEEGLFPLSRSLEKQEDLEEERRLFHVGLTRAKERIFLSYVNFRRRFEGMLNL
                     PSRFIDEIPEELYDFEDLCHRGQHKFYPNIKREYEDWEIADDILKVGTWVLHSNWGKG
                     RIIEKQGYGEDSKLTVLFSYGVKKKLLAKYANLEILQK"
     gene            complement(39394..40002)
                     /gene="wrbA"
                     /locus_tag="VGB16_01520"
     CDS             complement(39394..40002)
                     /gene="wrbA"
                     /locus_tag="VGB16_01520"
                     /EC_number="1.6.5.2"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_009181555.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003955 - NAD(P)H dehydrogenase (quinone)
                     activity [Evidence IEA];
                     GO_function: GO:0010181 - FMN binding [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="NAD(P)H:quinone oxidoreductase"
                     /protein_id="HEX9916391.1"
                     /translation="MKILVVYYSMYGNTFKLAQAVAEGAKSVEGAEVNLRQVGELIPK
                     EIYSKIPQVIKAKEMQKDIPIATLQDLIEADAVLFGSPTRYGNMCAQLRQYLDTTGPL
                     WAKGQLIGKPAGLFCSTSTPHGGQETTLITMMLTLLHHGMIIVGIPYSEQRLFSTTGG
                     GSPYGASSVSGPKADKSPTEDDLELAKALGKRVAEIAKKLRK"
     gene            complement(40018..40323)
                     /locus_tag="VGB16_01525"
     CDS             complement(40018..40323)
                     /locus_tag="VGB16_01525"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEX9916392.1"
                     /translation="MFIITYTYRVPFGRKDEFLEVQKQAKSLYARYGCSGYEVYEEKG
                     SSGFCMEVIYFESEEHYQKVRQQVDADPQINTLWEKFCGIAHARENPPIVKKYNRIL"
     gene            complement(40333..40695)
                     /locus_tag="VGB16_01530"
     CDS             complement(40333..40695)
                     /locus_tag="VGB16_01530"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEX9916393.1"
                     /translation="MKSKFFIFLFLFSFFPVFILAQPLGFNKITDDLSLYKNFLDELD
                     DAGQTLQIVTVNSFKLYFWFDLEICLDYNWYDSGDDYYMEMGLVKPVFKNFKLNYQRI
                     YGTFVNKPINQVGIRFSF"
     gene            complement(40916..42451)
                     /locus_tag="VGB16_01535"
     CDS             complement(40916..42451)
                     /locus_tag="VGB16_01535"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_006929957.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005524 - ATP binding [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="YifB family Mg chelatase-like AAA ATPase"
                     /protein_id="HEX9916394.1"
                     /translation="MLSKILSSAVLGIDAYVVQVEADISPQLPSFTTVGLPDNAVKES
                     RERVQSAIKNSGFTFPNKKITVNLAPADIKKEGSSFDLPIAVGILSATGQVSKENFED
                     YLILGELSLDGAVRPIHGALPMALSARKNNIKGMILPKENAKEAAMAKDVEIYPVSNL
                     KEAVSFLENELSTKPFTIDVNQVFEEALDYKLDFSDVKGQEHTKRALEVAAAGGHNII
                     LLGPPGSGKTMLARRLPTILPNLTLDEALETTKIHSVAGFLPSNAALIATRPFRSPHH
                     TISDAGLVGGGKIPRPGEVSLAHHGVLFLDEMPEFHKDVLEVLRQPMEDGRVTISRAL
                     MSLTYPAKFMLVGAMNPCPCGYHGDLIHDCSCSPLQIQRYVSRISGPLLDRIDIHIEV
                     PAVKFKELSGEPTGENSTLIRERVNKARKTQLERFKKEKHIFCNAHMDSKDIRKYCPI
                     DESSKELLRMAITRLGLSARAYDRIIKVARTIADLENQKEILPQHISEAIQYRSLDRT
                     LGM"
     gene            complement(42656..43021)
                     /locus_tag="VGB16_01540"
     CDS             complement(42656..43021)
                     /locus_tag="VGB16_01540"
                     /inference="COORDINATES: protein motif:HMM:NF016370.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003677 - DNA binding [Evidence IEA];
                     GO_function: GO:0004519 - endonuclease activity [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="endonuclease domain-containing protein"
                     /protein_id="HEX9916395.1"
                     /translation="MKRKNVERSRNLRKNQTDTERKLWSILRNRQLASAKFRRQFSIG
                     RYILDFYSPKCKLGIESDGGQHYEDDHRSEDELRTKELSQLGVRILRFSDSEILKNIE
                     GVCRVIQENIEKGKLTPSP"
     gene            complement(43121..43609)
                     /locus_tag="VGB16_01545"
     CDS             complement(43121..43609)
                     /locus_tag="VGB16_01545"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEX9916396.1"
                     /translation="MLKTPKGLELPEIPLDKKYRTSTKALLSRINTIYKGLYKKFGEA
                     GLDLIQEVSHTYGKQVAEFAKKKMGNPTPQEVGLYLLRIFSMIDCEGEVSEFSDDKVV
                     IKLYQCPYPFDDPKICQAHTTMERTLVEELNKDLEYVISESIPSGGKYCVHVVKWKEK
                     NR"
     gene            complement(43638..43892)
                     /locus_tag="VGB16_01550"
     CDS             complement(43638..43892)
                     /locus_tag="VGB16_01550"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011309550.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005525 - GTP binding [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="EF-Tu/IF-2/RF-3 family GTPase"
                     /protein_id="HEX9916397.1"
                     /translation="MPEEIVGKIAGYFKKIDVAAIDITHGILKVGDTIHIHGHTTDLT
                     QKVESMQIEHEQVQEAKAGISIGIKINGAVRHNDTVYKVV"
     gene            complement(43951..44898)
                     /locus_tag="VGB16_01555"
     CDS             complement(43951..44898)
                     /locus_tag="VGB16_01555"
                     /inference="COORDINATES: protein motif:HMM:NF033709.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="PorV/PorQ family protein"
                     /protein_id="HEX9916398.1"
                     /translation="MRKTKIAILTLLTGVFLISYAYAGINSNAGTTAYSFLKIGVGAR
                     AVAMGGAFVGLADDQSAIYFNPAGLTQLPNRSFTTSYNNYLTDIQSGFIGYIHPYDEK
                     TRLGISINYFNYGSFDKTDQYGENLGTFGAADFALIATYARKVHPLISLGVNAKFILE
                     KIDNYTSDALALDIGAFHKSEKKKIQVGVMVQNLGYQLNGLSKEHKESLPAEIKVGVS
                     HYVHGLPLLVAVDGAKPFDNDIYFSLGAELSSFKPAFLRMGWSSFGKNYKTGSDKDNL
                     AGFSFGLGVNWKVYRFDYAYSSYADLGGVHRISISGDLK"
     gene            complement(44960..45442)
                     /locus_tag="VGB16_01560"
     CDS             complement(44960..45442)
                     /locus_tag="VGB16_01560"
                     /inference="COORDINATES: protein motif:HMM:NF016290.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="LptE family protein"
                     /protein_id="HEX9916399.1"
                     /translation="MKTIKYVLFPVLLLAISCGPYSFHGTSLPGVNSIAIPPFENQTA
                     EYGLSESITEKVVDGFVQNHVLKVLNEKEADAILYGTITKYQRRAYTYDEQENIKEYI
                     SEIYVKVWLEDKNKKKNLWEENMKGWGVYSVTEEESQGKERAVEKLVEDIINRTVKSW
                     "
     gene            complement(45846..47105)
                     /gene="hutI"
                     /locus_tag="VGB16_01565"
     CDS             complement(45846..47105)
                     /gene="hutI"
                     /locus_tag="VGB16_01565"
                     /EC_number="3.5.2.7"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_020250517.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0050480 - imidazolonepropionase activity
                     [Evidence IEA];
                     GO_process: GO:0006548 - histidine catabolic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="imidazolonepropionase"
                     /protein_id="HEX9916400.1"
                     /translation="MIADLIIKNVGQLLTLRSSKRGPRAGKNMQELGIIENGTVTIKG
                     NRIISLGKTDKVLSKIKVGKNTKIVDAKNRVVTPGLVDCHTHPIFANTREEEFELRIK
                     GKTYEEIARAGGGIKSSVRKVRKLSKEELIKLAIPRLDRFLSYGTTTIEAKSGYGLSL
                     KDEIKILEVIRQLNRIHPIDLVPTFLGAHEIPEEYKDNKEGYIRLLIEEMIHLVAKRR
                     LAKFCDIFCEKGVFDTKDSKKILLAAKKYGLVPKLHAEQLSHFGGAKLASQIGAICAD
                     HLENVTLTDIKLLKKRGVIGVLLPGATFGLGLKKYAPARKMIENNLPIAISTDFNPGS
                     SMSESMPMMMSLSCLMMKLTPAEAIVASTINPAYALNMENQIGSIEPGKKADLVIWEV
                     KNYKEIPYHYGINLVEKIIKNGKVLNL"
BASE COUNT        13057 a         9664 c        10665 g        14043 t
ORIGIN      
        1 gaagcgcgag aaatataatc tgcgacaagg tgattttaaa agggacgatg agaagcctgg
       61 agaaaaaggt ggctggcgat ttgccaattt acgtaaagca gatagcttcc gggattgccc
      121 aaagccatgg agcagacttt aaggtcgcct acaataaagg ttacccggtc ttaattaatg
      181 atccaaaatc aacagatact gccagggaag ttacaagaaa cctctatact aaggaagccg
      241 tatatgagat agaaaagccg gttatgggtg gtgaagattt tgcccgcttc ttagaaaaaa
      301 ttggtgggac gatgatgagg ttgggcataa gaaatgaaaa aattggagca atctatcctt
      361 ggcaccatcc caaatttaaa atagatgaaa aggcattgaa attcggctca gctgtcctgg
      421 cgggcatagt tttgaagtcc ttacagatta agtgaagcct ccctatggag acttccaaaa
      481 cccttgtttt gaacccgcct tcgtcccgac ataaggtcgt gacttcggcg aggctcgcct
      541 cgtcatttcc gccaatggcg aggctttctg gcggaggcga gtaaatgaga aaaatcccct
      601 tactacttgc ttttttcctc cttttttttc atcaagtcag cttctcttat ctgtataacg
      661 aaggcgattg gacaagctac acaatgacgc gctatgttac ttccatcgct atggatgata
      721 ggacagttta ttttgggaca agtggagggg ttatacgtta taacaaaatc gaaagggttt
      781 gggaagaccc ttttaccacc agcgatggtt taccagataa cagagtgaac agaatagctt
      841 atgacaaaaa tgaggatgtg atttgggttg atacccccct tgggttcgcc tcctacaatc
      901 ccacttttaa agaatggaga agggaagcta gttttcctct ggaatgggtg agaagcgata
      961 gggatagtct gaaattgcct gattttttta tggagtttgg ttatgatttt tttcccgatg
     1021 gttatatcct cgaccaaaga ctttccagat atgacataac cgactacctt agagatgatt
     1081 ggggagataa tctgtggctt gctacctggg gcttgggagt ggctttcggc tctttgcgaa
     1141 gctggaactt acagctttat acttttggtt tatatcaaaa tgatgtcaaa gatatttgca
     1201 tagatggggg cgtagcctgg tttggcggaa atgatatttt tcaaaataga gtgggaataa
     1261 ccaaatatga tagagataaa gataaatggg aatatttcgt tgaaggttat gagaataatc
     1321 tgggaagtgc cagggttaat gccatacggg caagccagaa tttcgtctgg ttcggaacta
     1381 cgcagggatt agaggtttac agcaaaaaaa aagaggaatg gagtttttat aaacgagcca
     1441 aggaattaag gaacagctac ataacctgtc tggagtatta tcgcgggtcg gataaaaaag
     1501 atgagggcat cctttatgta ggaacataca tcggcttatg ctcttacaag gtgaaaaaag
     1561 attcgattgc ccaatttaag gaccaaaata taagttcaga gtatatcaat tgcctgaaac
     1621 tgtcacataa ctatctctgg gtggggactc aaatgggaac ttatcgtatt ccccttgcag
     1681 gaggaaatgc aggggtattt tcaactccag acggaatcac gaacagcaat gttaacgata
     1741 tagaagaaga taaggacaaa gtctggtttg ccactgatag gggaatattg ggttacaatc
     1801 caatcactga ggaaagggaa gtttatcaga tatcaggcaa ctatcctggg agccaacctt
     1861 taagattggc gatagatgga aagaatctct gggtggggac gaaaaacggg gtgtggagga
     1921 tggataagga gaaaaaaatc tgggataact tcactatttt tgatggtttg atagatgacg
     1981 aaatccaggc tatggttttg gatggagact acatctggtt tggcactcct aaaggtgcaa
     2041 cccggctcta ttggaataat ccccgtatct tgagatgaat atgaaactaa aacaggtagg
     2101 agagttcggc ttaatagatt taataagaaa aagagtcttc tccaaaggga aagaagtagt
     2161 cttgggcata ggagatgacg cagccatatt gaaattgaat aaaagtaatt tacagctctt
     2221 gactactgat gctttggtgg aaaaaatcca tttcgatttg gactacttcc cgctggaagc
     2281 ggttggctgg aaagggatgg tggcgaattt aagtgacatt gcggctatgg gaggagtgcc
     2341 taaagcagca gttgtcacct tgggaataaa taacagattc aaagtggagg agatattatc
     2401 cctttgttcc ggaatatcca aagcggcaaa aagattcaac tgtcccatag tgggtgggga
     2461 cattgttctt tcccctcgaa atcttttctt gtctatcgct atattaggcg aagtcaaaaa
     2521 gggattggca gtcagaagaa tcggggcaag ggttggcgat ttaattgcag ttacaggtga
     2581 cttgggtagc tcctcggccg ggttagaata tttaaaatta aacaagaaac cacgttcgtc
     2641 ctcgcctagt ggctcgcctc tcggcgctcg cctctcggcg aagccgaggg aagccgaggg
     2701 aagccgaggg aagccgaggg aagcagaggg cgaagccact ggttttgcca gaaagttcct
     2761 ttatcctctt ccgcgtctaa aagaagccca actcataact cgatttctca aaccaaaagc
     2821 gatgattgac ataagcgatg gcttatcctc agaagcttat catttgactg tggagaatgg
     2881 attgggcgca aatatttatg aaaataatct tccgatcagc aagattgctc tcaagatagc
     2941 agaaggggat aaaaagaaag cattgcatta tgcttttaat tcaggcgagg agtatgagct
     3001 tttatttgcc ttcgatggaa caagaatcag tcaatttcgt aaactgagta gaaagataaa
     3061 gattagcatt atcggcaagg taaccagaga aagaaaaatc aggttgacag aaagtgatgg
     3121 caaatcaaga agtttatctt ataagggtta tactcatttc taaaagaggt ggttagggta
     3181 tgttaagagg caagttgttt ttttgttggt tcgggaattg gaattcccga accctttcgt
     3241 gtgggaaatc caattcccac acaaaccaat acaaacgcat tagtttacgt tcaatgaatc
     3301 tcccctcgcc ctcgcctcga ttcggcccgc ctcggcgagc catcggcgag acgggcgaaa
     3361 cgggcctctt tttcaaagag ggggaaaagg aggtctcccc ctttgaaaaa gggggacttg
     3421 atggttcgat atactcacca tcctgagcaa agtcgaaggg agggggattt taaaatagat
     3481 tgcatattgc tctaatatgt aacaacattt aatccgtttg ctataaatcg aggagtctat
     3541 cttccagaga ttatactcat ttctaaaagg aggccaaagc atgttaagaa gcaaattgtc
     3601 ttatctgctt ttcgcaattc tatttttgtg tttcggctta aaccagctgt gggcagatga
     3661 aggttctatt tttaccaggc agaacctgag agtaaatgga aaaattattg gcaagacgta
     3721 cgaagattta aatggagatg gtttagttga cttcttggtt ttttacttgg agggagaaaa
     3781 tgaaaacccc aactcgccta ggggcgaagc cactggaagg atggtcggcc ttttcgaaca
     3841 acaaaaaggt tcaggatttg acagcattcc caatcaaacc tttgaactga ataaaaaggc
     3901 ttcaattgtt gacctggtag atataaataa agataataaa aaagaattac tttttttggc
     3961 agatgatggc atctattatt attccctaaa tggtgattct ttcaacaagg agtcctcttt
     4021 attattctca actaatagtt tcttaccctc ttcagaagag aacattgtga tatgggattt
     4081 ttgtcctgat ttccaaaaca aaggtcaact ggtcataatt ccccagaaga gagggtttga
     4141 aatctggaca agaaacgatc agggggagtt cgttcgccaa gacaagttgg gattcaagcc
     4201 ggatatctct ctttccagta gccccggaga gttgaaccag aaaagaggat taattcattt
     4261 ttcctatgga ataccaggtt taatctttac agactataat aaagacggta aggaagatat
     4321 tctcatttta aaagaagata aaatccatgt ttttctctcg aaggaagatg gcttctttgg
     4381 agaacaagcg gataaagtgc ttaccttgaa accaaaagac aacaaagagg agaatataga
     4441 tttcaaaatc gaggatatca atggagatgg aataattgat ctggctgtga accaatccgg
     4501 tggagacctg gaaaagggtt tcaagagcaa aataagtata tacttaggca aagaagggga
     4561 agggctaaac ttagattcgc cgcatcaggt tatctcttct gaaaaagagc aatcctctct
     4621 tgagctttac gatttaaata aagatgggaa ggaggagatg atcgtatccg gggcgggctt
     4681 caacataggt tcaatgataa agattctgct ggccaagagc gtaaaacttg attttgatgt
     4741 ccgcagttta gggaagaacg atatctatcc ggaccaaccc aatcgtaaaa taaaattgaa
     4801 catcaaggta tcatttggta gctcagatga agacaatttg gcaatggacc cctccgggga
     4861 tttcaatggg gatggcttga acgatttgtt ctttgctggc ggtgacaata tgctgaagtt
     4921 ttttctggga agaaaagggg atttctttgc ggataagccc caacatgagc tgggcataga
     4981 gattccagct ggaaattgta agataataga tctaaataac gataacaaaa gcgacataat
     5041 tttcagtttt ggagataaaa aggaattgag gggcaaaatg gttatcctct tttccaagat
     5101 gtgagaagtt ttctgttgac tgtctaggtg attttagtta atttgaaaaa gatttaaaaa
     5161 aaagtggtgg gcggagcgca aaatattagt ttggggaggt ggggaatatg gaacacagct
     5221 ttcctgagtg ccaaaagtgc ggcaccggcg ttttgatacc gttgtccgat tacggtcggg
     5281 aaggggcaac cattcgcttt aaggcttggg tttgcaccaa tccggaatgt ggcttctcca
     5341 ttcgcataga caatggagaa gtcagctacg gcaagaccat tggccattcc accaagtgat
     5401 ttgcatattt taatttgagt tatggcgctc cgcccatcct acttttaagc gagaagtaac
     5461 ttcctatgga aaagatattc agcaatctcg gcttgaaaat agcagctctg tttttggctt
     5521 tacttttgtg gtttcatgcc accactgata aaaccttcat atatgatttt gacttgcctc
     5581 tggtcatttc taatatgcct tctgatttga tattggctag cccagttcct tccaagatag
     5641 aagtcaagat taggggcaaa ggcaaacaac tgataaaata ttttctctcc gacaaggaag
     5701 acgtggtgat tgatgttagc caattgaaat acagggaaat ggattacgta atcaggccag
     5761 aggagattcc cctgcctaag gatttgagct tagaggtgga gaaggtggat tcacccaagc
     5821 agataaaaat attattggat tacttctccg aaaagaaagt tccgattaga aaccagatgg
     5881 aagtcataac tcaaagcgga tatgttcaaa ttgactcgtt agggcttaag ccggacaagg
     5941 ttttactctc tgggccgaga acactaacca ggaaaattga ccaggtatat actgaaagaa
     6001 aaatcctggg tagtttgaac tcatctacct ctggcttggt tccattgatt gtgccggagg
     6061 gatttaatct gaaactctct ccggatgaag ttgagtacaa agtcttggtg gaaaagctga
     6121 cgcaaaagga attcactcaa attagtgtag agttagtaaa tctcccagtc aagaagaaag
     6181 tcaaaatcga gccaaagagc atagacctgt tcgttcaggg ggtttcacag aaggtggagc
     6241 aaattacacc tgacaaaata aaagtcagtt tggattgcga tagacttggc accaggaaag
     6301 aggggaaaat agtgccacag gtgaagctcc ccaaagaagt ggaattagtt agaatagtgc
     6361 cggatagcat aaagttcact gtagaataaa atgctattat ttttttcttc agcgaggtgg
     6421 cttttccctc gctattttat tttgagaata ttatgctcat tttaggtatt gaaacctctt
     6481 gcgatgagac ctcagctgct atcaccagag atggcaggat tctatccaat gttatttact
     6541 cccaattgat tcataccaga tatggagggg tggttccaga aatagcctcg cgccagcata
     6601 taaaaaccat tatgccagtg gtgcaggaag ccttgagccg agctaaaacc cctttgaaaa
     6661 aaattgaggg gattgctgta acttatggtc cgggtttagt gggttgtctt ttagtaggca
     6721 tctcctggtc aaaagcattg gcttattcac tcaagattcc gttgattggg ataaatcata
     6781 tcgaagccca tatctttgcc aatttcctgg aacatcccaa gcctatgccc ccttttgtct
     6841 gcttggtggt gtccggtggg catacccttc tggtggatgt caaagaaaag ggcaactatc
     6901 gggttatggg acagaccaga gatgatgcag ctggagaggc attcgacaaa gtggcgaaac
     6961 ttctggagat tggctacccg ggtggaccat tgatagatag gatatctaaa aaaggtaaaa
     7021 gagattttgt aagatttccc cgtgcctact tagaaaaaga tagtttagat ttcagtttca
     7081 gtggactaaa aaccgcagtg gctatctacg cttccgggtt gtcaaagacc aagataaaca
     7141 gaaataagaa agacatcgcg gccagcttcc aggaggcagt ggcggatgtt ttgatagaga
     7201 agagcataag agctctcagg gtagcgaaga gcaaacgatt gggcctggct ggtggcgttg
     7261 ccagaaacag tcggttaaga gagaagttgg aggcgaaagc taaagatttg aaattcgact
     7321 tatattatcc ttcatacgag ttatgcacgg ataatgcggc tatggttgcg gcttgcggag
     7381 atttttacct ttcccaaggg ataaagtcgg atttaagctt aaatgcaatt ccttacgcta
     7441 aaataagttc aatttaaact tgtaattccc tgtggctcgc gacagagtta ttttgtgtca
     7501 ttccgcactt gatgcggaat ccaggtttgg ttgttgtggt tttatcgttc tctttctgga
     7561 ttcccgcatt cgcgggaatg acagcgcaat acattcctac acgaattttg tgtcttgaga
     7621 gtgggagatt ttattgtatt tcctctccct tggtacgaac catgacggtt cataccttgg
     7681 ggggagaggg aactggcaag aatagtatta gttttctacg ggacattctg gtatgtcgcc
     7741 tgcgtttcta ccccccccca ctctctgggg ttttttatta aaatcatttg cctttttggg
     7801 tataagggtt attatttgtt atgtcaatgc ccttaaagtg aagcctcccc ctacgggggt
     7861 ttcccaaata taaaaggccc tcggcttcgc cgagaggcga gcgaaacttc gcccgcctta
     7921 agcgaggttc gccaaaggcg accgaagcat tcttttaagc ccgccttcgt cccgacataa
     7981 cgtcgggact tcggcgaggc tcacctcgcc atttccgcca aaggcgagac ttcgcctctt
     8041 tggggcgagc atccgcctac aggggctttc tggcggaggc gagtaactca aaagagaaaa
     8101 aggaataagt tgaagagatg actaagatat tgcttgattt actaataatg tttgccgcag
     8161 cgaagttgtt gggcgaggtc tttgagcggt ttaaattgcc ggcggtgatt ggtgagattc
     8221 tggctggggt ttgtctggga ccctatgtct tacatatcat cactccgact gaattctatc
     8281 atgttttggc tgaaattggg gtgatcatcc ttcttttcta tgtcggtctc caaactggta
     8341 tagatgaaat gatcaaggta ggaaagaact ccatttcagt agctatactg ggagtcgtcc
     8401 tgccttttat tctcggatac gcttatattc tatttagtgg gcatagtgca gttgaaggaa
     8461 tgttcattgg tgcggctatg gtagcaacca gcgtgggcat aactgccagg gttatggccg
     8521 atttgaatgt tttagacaaa aaagtgagca aggtcattct cgccgcggca gtggcagacg
     8581 atatcatggg gatgatcgtt ttggctatag ttactggtat gggaaaagga accctgtctt
     8641 atctgaatat aattctggtt attctggagg cagtagtatt tgtaatattt ttgatggtta
     8701 ttgggcacag agtaatcagg cgcgtaaccc ctggagtgga ttcgcttaag acaagaaacg
     8761 ccccctttgc ttttgctata atcttgtgtt tagccctttc agttttagct ggatatatca
     8821 aattggcagc cattgtgggc gcttttatgg ccgggatggt tctctcagag tttaatatcc
     8881 gcttccgttt aaccctaaag atggaatccc tctatgattt tttggttcct ttcttcttcg
     8941 tggtaatggg agcaaatgtg gatttgacag tattcggcaa atggagcatc ttgggtttgg
     9001 ctttgatttt gacttttctg gctattatag gaaagttgat tggatgtagt ctgggagcaa
     9061 tgaatctggg tttcagacag gcgaccatta ttggcgtcgg aatggtgccg cggggtgaag
     9121 tgggaatgat agttgcctca attggcctga gtttggagac tatttccaga gacctttatt
     9181 cagtggtcat atttatggta gtgatgacaa ccgtcttgac tcctccgata ctgaggatgc
     9241 tcttgagcag atggagagga tttttatctt aagttgtaga ggttagattt atcccgcctg
     9301 tggcgggatt tacccgacct aatgcctctc ttaaacccct cctgacctcc ccttatcagg
     9361 ggaggaaaac gtttcgcatc cacctgacaa ttaggtgagg aacggttttt gtccccctga
     9421 caagtgggtt acagggggtt aacagtttgt aggtcttgat gcttcaggaa taggaatttt
     9481 ccgcttttag cggaatcttg gtgacccgaa ccgtatcgta atttcgtgca ggaattccaa
     9541 ttcctgcacg aactaaacgc atcggtactt ctcctccgcc aaaggcgaga gatcttgaga
     9601 ccaatgccga tacatcaaga atctccccta accctcgcct cgattcggcc cgcctcggcg
     9661 agccatcggc gagacgggcg aagcgggcct cttttgcaaa gaggggaaca aaaatccccc
     9721 tttgacaaag ggggaaaaca gggggatttt atggtttgca ggtttttccg gtttgcggat
     9781 tattctgatg tttcgggaat aggaattccc gaaggcacct ccatcggcat ttctcctccg
     9841 ccaaaggcga gagatcttga gactaatacc gacgcatcaa acggggtaag caatggctgc
     9901 gccgttgctt ctttgggaac ggtgccgtaa accatgcggt tcatggcggt gccatgaacc
     9961 aatgcggttt atggcggtgc aaccgttccc tacccgctgc ccaaggtggc ttaagtgtca
    10021 atcttatttg ctcagaaggc tcgccgaaat agagctaaca cattttacag agaagttaca
    10081 tttttgcttg acaagaaaga ttggcttatt atattaaatc taattttgtg gctttaaacc
    10141 aaaatagggc ttttgaaaag tggatgtaat tttgatgtgt ccctctttga ggagaggctt
    10201 tagtttttcg aatcttttct atcatttcaa gaaagaaaac accaatctga gaaatcaaaa
    10261 atagttagtc agtttcagtg aggggatttt gttcgtaaat tcttaatata atcgttacag
    10321 gaggtgccaa gtgagattta caagtaagct gcttataatt tcgattttgt ttattctgtt
    10381 tgttattccc tgcggtgttg catatagtca ggacccaact atatggtatg tgacttttga
    10441 tccggggata ggagattaca ctttgcctaa acctaattct atttcagctt tcatctggtt
    10501 ccctgaaata gcagatatgg acactacgat ttattatcct ttaactttta cattagacgg
    10561 tttactcata tataattggg agccaaattc agagtttgtc caaagcggat ataatgttac
    10621 ttcaaataaa tatttccata gaccggaata cgctagctat gatgttttgg attcgattcg
    10681 attagatatt gagttgactg atagtctgtc acttggggcc catgagtgga aagtagagct
    10741 gtggcttgtg gattggccct ttgagccttg gtttgcagga gcaaagacct tttatgctga
    10801 tactgtctgg tatgctccat ctgaagcaac atctctcgct ttgattcctg aaaaggatca
    10861 ctacacctgt aactcaccta aatttgaagc ggcattcagt tctctgcccg gataccaatt
    10921 gattgatact actgtaaagt ttactctata tgctcccaac gaaactttag atgtctatga
    10981 tggctccaga gatacagcag ttttagatgg gtattatgcc agatcggatg tcgctcagtt
    11041 aaaattattc attgaagttg acgatgagca aaccctttta gatagcagtc agtattccta
    11101 tgagatttca gccatagccg tatctcttac gttaccacgg ataatatact ctaaggagga
    11161 taccttttca gtcggaaatc ttacgacgcc caaggagatc atcccacagt ctttttccta
    11221 tgttggtcct aggcccgaat tctattatac gattggaagg tccggcactg ctctggatac
    11281 cactaacatc tacttggatt tattctacgt gacgttcgat tatgacacag tcggagatag
    11341 caccatagag aagagaaact atcttaagac tatctatccc aatcagatgt cgaaattcgc
    11401 aactgataca gtaaaagtag caaccaatct ggaactgatt gacatggctg ctatggatgt
    11461 gttggtttgt ggacaagcag accaatatgc gcgtcaaggc tatgagaatt ttgtccccta
    11521 tactttaaga atggggtata tggataccgt atctaacaaa agaggaccgg cttataaaag
    11581 atttattgta gacagggttt cgccacagat aactatgctg gattcaactg ataaggcgtg
    11641 gaaattccac atacaggatg gcggttcggg agtgaacaac agctcagtcg tagttacgca
    11701 gaatggtcaa aaagtaacaa atgatgctct tgttcagtat aacccaacta cgcacatttt
    11761 aacttattat cccctgaccc tggatgcact gtttacactt gaagccgaag ataatgttgg
    11821 aaatgagggc agttattcca cttatgtgaa gagtggtaga ttgattgctt atgatatgca
    11881 tagttatcct aatcccttca atcctaccaa aggggaagaa gccacaattg tcctaagctc
    11941 aaatagacca gacaatcaag ctctggagat aacagccagg gtctgtgatt tagcagggaa
    12001 acatgtagcc accctgaagc agaatgcagg taagctatta tggaatggca agacagatgg
    12061 aggagaattg gtggcaaatg gagtatatct gtgttacgtg agcataaggg atttgaatga
    12121 tttaactgtc cagaattatc ttttgaaaat agccgtggtt aagaaagatt agttagatag
    12181 attctcactc aggaggtggt taatgagaaa gttgagcaag tcggttatgc tgataggtgt
    12241 gatttttctg atattcggct ggagtggcgt ctcttttgcc gaatatgagg tattttttgt
    12301 tccatccttc caagatagtt ttaccacgac aaggcctgaa atcagaatca agtttcccaa
    12361 gggaggggtg ggcccaacta aggtagcaat tgatacacta aagcgttgcg caaacgagcc
    12421 ctactatggt ttcattgatt ctactggtag attcccaaga ctagattcga tatattacac
    12481 aaaaacaagt ccagctgata caatagttcg tgccgggttc ggtgactcta tgtatcagac
    12541 agatgggtat aatgtcttgt gccttttctg ggataccgcc acggcacttc cagccagaga
    12601 ttcagcaagt ggcaaaccgg cctatgcact aaaggttgat tcagccggca ccgtcttccg
    12661 ttatccgcta accccctgca agtataatgt ttcccgtgct ggcgagcttg tggttaaacc
    12721 ggctcataag tccaacgact atactaaccg tactcctata ttccaagcgg agttctctat
    12781 ttttaaggag gtcaaaggtg atactacctg gtatgatgga gtatttcttg acaccaactt
    12841 aacggttgtc ttaaaagcta ctgggataac agacactttg tacgattaca agagatttcc
    12901 gcagttgcga ccaggtctga ttggtaatct aagaaaaatt catccgggta agatggtgtt
    12961 tactatcagc tggcttgaca cctcttacta tctacctctg gacatgacca aagcatatat
    13021 tgacagcgta acgggagggg tgtgggatta tcaaggtctt agtattattt gcggctctcc
    13081 tccacttggt tacagtccta ggggagtagc tgacactttc tgggttgatg gaactccacc
    13141 cgagatggaa ttacaggaga tgaactacgt cagcaaatcc ccatatttct tgctcaaggc
    13201 atcggatgcc aaagccggag taaatactga ctccctcttt gttgacctgt ataaagtcga
    13261 actttatggt gaagcccctc cggatgatag cgtggaagat agaagttatc tgaagagctt
    13321 gcaggttgaa gggctcgatc catcattagg gagtgtttta gtgaataccg gagttgaatt
    13381 ggaagatgtg gagggtggta tggctttgga tgtctgggtg ttcaatggta gaccgtttga
    13441 tgtgaatgag tatatgacaa acttagatgc cgtctatccg gacagcttag gtccgatgga
    13501 tggggcagga aatctgatta cccctctgta taaacgtttt tatgtggacg ggataccggc
    13561 gaacgtaaga ttatcttcaa actccactca cgagattagg atttttgaaa tctccgatac
    13621 ctcctcgtgt tatcaagttg atactttagg acacgttgaa atcctttgct caggagtaaa
    13681 cactgccagc gtcaagttgt acgagaattt ccaactagtc actcctgatt caaccaagta
    13741 caactcgtca agtggagctt actctgtcta ctatagtcca acttcggaga atgtatggat
    13801 aaatctggat gtggcagaca aagtgggtaa catagctact tatcagaact attacgaaga
    13861 tgaccagctg aacttggtgg agccgcataa ttatcccaat ccttttagcc cggttagtcc
    13921 catagttgac caccgcagga cagtcataga cccggggtta accagaacaa ctgatatgga
    13981 attgacaatc gatatctatg acttagccgg acaccatgtt gtttcggtta ctccgaatat
    14041 ggcggatgga ggcagagggt attgggatgg taggacaaaa gatggcactc tggtggccaa
    14101 tggagtctat ctgtgctata ttcaggcaag ggatacagcc caaaataaga ctttgactga
    14161 agtaattaaa atagcagtgg caaaaaatga taagagaata ttttaaccat taaaccaagg
    14221 gagagtgaga agatgtcaaa cttcattaga aacattgttc tccttgctat tgtttttagt
    14281 atgctttgtg ttcctcccct cttagcagat gataaccatg ccatgccagt gctgagaatg
    14341 ggtgtagggg ccagggcttt gggtatgggt ggagcttata ttgcggaggt gaaggacgct
    14401 tcagccacct attggaaccc agctggctta accgagatgg aacatctcaa cataaccggg
    14461 atgttctcat ccgagatgtc ttatgagagg aatcataatt tcctggctta cggtagaaaa
    14521 ttcggtttcg gcgctttggc tatgggctgg atatcccatg aggttagagg agttcatgga
    14581 tatagctatg agaatctgcc taccagcaca ttcagggagg tagataatgt atttatgttc
    14641 tcctacgcca gaaagatcgg aaaattctcc tccggagcaa attttaagat agttgaccaa
    14701 aggatgggag attacttaag cgaaaccggc atcggattcg acctgggggt gcgttattct
    14761 tataacgacg atcttgtttt cggtgcagtg gtaaaagata ttgccactaa ggttggcggc
    14821 tggaccggaa aatatgaagt ctggaaggat agagttccag ctaacttcgg agtaggagca
    14881 gtagtctatc cttacgctgg tttcactttc ccggtggatg tctacaaagt tgagggcaga
    14941 gaggatatgg cactccattt cggcggagaa tatacctata aacttgaaga aaaatactcc
    15001 ttcagccttc gcgctggctc aaatgacggc caaatagctt ttggatttgg tctgaagttt
    15061 gtgaaattca ccatagatta cgcttttatg aacgaacagc aggatttctt aaaggagaat
    15121 cacaaattct ccttctcggc tgatttttag aagtcgaaat gagccaaaac taaaaaccct
    15181 cccgcttcag caggagggtt tttgtattgt aggggtcgca ttccctggcc atggaggtcg
    15241 agcggggtgt gaaccgttct ggttcacacc ggggaagaac gggtaggctg acttggtccc
    15301 gcattttgcg ggatcgggtg aggaaacccg gcaggtacaa tggtccgcac agggataaat
    15361 tccctgtgct acgaatataa catgtcgtag gtgtgggaat ttcgttggtt cgggaattgg
    15421 atttcccgaa ccacttgtga gcgggaattc cacaggtaga caaatgtatt aatcttcttt
    15481 gtgtcgggta gctcgccaaa tggctcacaa cccgacctac agtgtctaaa gaaaatagag
    15541 agaagatact gggcaaaggt ttgaagggag cttaccgaat cataaatcaa agcgctgtaa
    15601 aaaacccgta ataaaggaag aatttatgaa aacagaacaa tataaaattc aggtgccaga
    15661 gggggctaat ctcatcttgg ggcagaccca ttttattaag acagctgagg acttatatga
    15721 gattctagta agttcggttc ccaacataaa attcggctta gctttttgtg aagcgtctgg
    15781 cccctgtctg gtcaggcacg aagggaacga tgagaaatta aggagtttgg ctaagaaaac
    15841 ggcttttgag ataggtgctg gacatatttt tgtcatcttc atcgaagatg cttatcccat
    15901 aaatgtatta aggggaatca aggagtgcgc agaagtatgt agcatattct gtgccacagc
    15961 taacccagtt gaacttctaa tagcccagac cgaacaggga aggggagtaa ttggagtggt
    16021 ggacggtttc tctcccaaag gcatagagga agacaaagat attaaagaga gaaaacagtt
    16081 cttaagaaaa atagggtata agctctgaat taacgcagga aaattaactt tttggtctcg
    16141 gagaattcct tagttttcag gttataaaaa tatattccgc tcggcaagtc catacctgaa
    16201 ttgtcctttg cattccattc tatccggttt tgtcctgaaa gcatattttg gttcaaagtg
    16261 aaaacttttt gtcccaatac gttgtagatg gttatgttaa cttctgcttg cttatccaga
    16321 tagaaagata tagtggtact ggaattgaat ggattgggat aattctgctc taatttaggt
    16381 cctttagggg ggttatttgg atcctcacca gtatgctcct ttaactcgat ggaataagta
    16441 taatattgtt ttccatcctg aacataataa tacccttctt tgatttggtc ttccactgca
    16501 acgacagtca agattgcttc ttgaatattc ttgccgaagt cggatatgct aacgcttatc
    16561 ccaaaaggga agttactaag tcgttcaact gatataactc ctcccttttc atcataagtt
    16621 acgattttac ccaagaggat agtatttagt tcggggtcga tttggtcctt ttgtagtctg
    16681 aagtcgagtt gataggtttt gccattatct aaggaagcat cagctgaaag ctcgatatag
    16741 tcagaagaat aaagataaga aaatagacta tctttaccgt aaattactga gtaggttttt
    16801 tcgttgttta taaatgaagt atcaaccaaa tgtagagccg gggcatagcc acaattctgc
    16861 agggaatctg ggtttccgta atttatcttt agataaccta tttcgttttt cacctccaca
    16921 ttcttatatc cgtattttcc gtcttcaatt gtggtatcat ctaaaagatt tgccattgcc
    16981 cagtttgtga aaacctctct taagaggtcg tctccggata aaccatatct tttgaactcg
    17041 tctttttcaa acattcttgc caacgaatac ataccactga aagttcctga ctcccttctg
    17101 atatactggt accatagatc gtcgtggatt gaatctgtca agccaggaaa ctgctcatat
    17161 agataaacac cccacaaata ccaatgccaa tatttgctgg taagtggagg tccagatgaa
    17221 aaccaaggaa ggggattatc gaatggtata ttgtaattga atactcctat attcactttt
    17281 ttgtatccgg tgaggaatcg agatagttcg gacttagttt cattcaccca ctcgttatca
    17341 tagtttgtgt ttccctgatt acacaaatgc tgcaattcat gggcaaagag gcccataatc
    17401 aaagggtcac caggatacaa aagccctgtt ccatatctta cccgaatggc agaatagaag
    17461 acattcctgc cagcatacca accataaccc ttgcagaagt aactggggtt gcttccatta
    17521 cagtcttgtg cgtcattcgg gtcatagaga tagtatttta tcttggtata agtactatcc
    17581 cagatggtaa atcttatccc ggcttggctt gagtctaaat cattggggtt aaaatagttc
    17641 cagacgactg gaaatatgtc ggaattgttt gtagtgtcat catccaagat gagatttatg
    17701 aaatcggaag tatatttgtc ctcccagttt tgcacaactc cgtccgttac atatagctca
    17761 aagcaattgt caaacacagg gaagttgctg gtatcatact tgtggaaggt aggatcggtt
    17821 ggactctgag agaatgcagt gtcaagaaac gcaaaatata ttaacaaaag caggatggtt
    17881 ttcttcatta tgaaagaata tcagagtatc cccgtagggg ggatgaatga catttctata
    17941 agttccctct cccttggggg agagggttag agcctgccct aaagcgaagc gaaggggtga
    18001 gggggataac gaagatgatt cttacccctc tctccgcctt tggcgggatt ttcaacttag
    18061 tcctccccct caagggggag gagatgtttt ggatgccccc gtagggggaa agctcactta
    18121 gaacgggatg tcgtctcctt cggcctcttc ggtttctggt tccggaggga tctcttcctc
    18181 tgaaggccct tcttcctttc tgcctaacat ctgcatgcgg ttagccacga tttcagttat
    18241 gtactttcga ttaccatcct tgtcctcgta tgaacggctt tgaattcttc cctcaatata
    18301 gacggggctt ccttttttga gatattcctt gcatacttcg gcctgtcttc cccaagccac
    18361 aatattatgc cagtcagttc tctgttgcaa ctggccgtct ttatccttcc accgctcgct
    18421 ggtggcaata gggaatttcg ccactggttg accactgggg gtgtaacgta agtcaggatc
    18481 tctgcccaaa tttccaatta agatcgcgcg attaacgcta gccataaatc ctccttaaat
    18541 agataatcag aacttttctg ttaatataca gacttttggc caattgtcaa taagttaaat
    18601 atcccaactc aatttatggc ttgacttttg ggcatggtct cttataattt aaagtagtta
    18661 taatttatct attttatcaa aatgaataat catcctcaac ttccctcggc ctcgccgaga
    18721 ggcgggcgtc gagagacgag tccgcaggtc tccataattg ttccagctta caatgaggca
    18781 gaaaacatac ctcttctcat ggagaaattc tcaagaatgt ttcagtcctc cggcctaaaa
    18841 ggagaggtga ttttggttaa tgacggttcc acggataata ccttggctca agctaaaacc
    18901 tgtcaagaaa aatatggttt cttaagagtt gcctcgcaca aggttcgaaa gggaataaca
    18961 gatgcattgc taactggatt tagtgattcg caaggagagg ttttcgtatt ctggccagct
    19021 gacctgcaat atctgccgga agatatacct catatgatag agaagataga ccagggtttt
    19081 gatatagtta ccggctggaa acaggggaat tatgggttaa aagggtttgt gtctttctgc
    19141 tataacctcc tctcccgcat tctgtttggt ataagagttc acgacttgaa ttcggttaaa
    19201 gcttttcgcc gagatatttt cgaggatgta actctgcgca aggattggca cagatatatg
    19261 gttgtcttag ctgcggagaa aggatacaag atcgcagaag tcagggtgaa gttatacccc
    19321 cgcaagtatg gaaaatcgaa attcggtttt tggcgaatcc ccatagggat actggactta
    19381 ttcagcgtta agttccagct ctcttttttg agaaagccga tgctcttttt tggatttttg
    19441 ggtttggtat tatttttttt gggagtggta gttgctggtt tggcgcttta tctaagattt
    19501 atagaacatg aaggttttag gccgcttttg tatttggtta tcttgcttgt gctttcaggc
    19561 atctcatttt ttgccttggg atttctggct gaagccatag tgagcttaag ggaagagttg
    19621 agagatatca aaaacaaact gatcaaatag tgtcttaacc ttgcctttct tttcttaccc
    19681 tcttttcctt aaatccaatt tgatttcagg ggtgtggact caagatccaa agagttggct
    19741 ctcaggacaa cctgagtagt agtgtttgtc ccagccactt tagctctctt gactatcttt
    19801 tccttgagct aataactgtc ctcaattaga tgcgaataag aatgattggg cgtgcacaac
    19861 ggttcgtgag gttgggtacc aacagtttga gaattggggt aatctatatt gacagacgga
    19921 tatttttatt tatattgata cttcaacctt gaatccctaa cactcatttt tcatgaaaac
    19981 cttagttatc atacccactt ataacgaaag tacaaacata gccacactcc tccctcagat
    20041 tttagctcag gacccgacaa tcgatgtttt ggtggtggat gataattcac cggatggaac
    20101 tggaaaactt gtggataaga tgacaggcga aaacagtcgc attcaggtaa ttcaccgcca
    20161 aaaaaaaagt ggcttgggaa ctgcttataa ggctggcttt aaacatgccc tggaaagggg
    20221 ttacgattac atatttgaga tggatgccga tttctcgcat gaccccggat atatccctca
    20281 ttttctggaa gcgataaaag aggctgactt ggttttgggt tcgcgttaca taagaggggt
    20341 gaatgtgata aattggccta tgtcgcgcct gcttttatct tactttgcca atatctatac
    20401 ccgctgggtg accggcttgc ccataaggga ttccactggt ggattcaagt gctttaaaag
    20461 agaggttttg gaaagcataa atttagatag agtcaagtcg aatggataca cctttcagat
    20521 agagatgagc ttcagggctt ggaagaaggg gtttcgcctg aaagagattc cgatcatctt
    20581 cgaggacaga agggaaggac aatccaagat gtccaagaag atagttcgcg aggcagtatg
    20641 gatggtatgg aagttgaggc ttctgagtat tctgggcaag atatgaccct atctttcgac
    20701 gtttcaatta taattgtcaa ttataatgtg aacaaacttc tgtttaaatg tctgagctcg
    20761 gttttcagaa ccaatagcaa aaaaaagatg gaagtgatag ttgtggataa tgcttcccgg
    20821 gacaaaagta cagaaacagt gaagaaggat ttcccgcagg tgaaattaat ccaaaacaga
    20881 aaaaatctgg ggttcgcccg aggagctaat cagggaatta aggcaagccg aggcaggtat
    20941 atatttcttc tgaatccaga cactttaatt accgccggtg aactggataa gataatagac
    21001 tttatggaga aaaacaaaaa agttggcatc tgtgggccaa agatggtaaa ttcacaagaa
    21061 aatatacagt attcctgcag aaaatttccg aattatctca cctccatatc ttccagccag
    21121 tcgctattgt ttaagctttt tccgagaaac cccctctctc aagaatatct tctcactgag
    21181 aaaagtcacc gagaagagat ggaggtcgac tgggtatccg gttccgccct tttagccaga
    21241 aaagatatgc tggaccaaat cggaaactta gatgagaact ttttcatata tgtggaagat
    21301 gtcgatttat gctatcgcgc caaaaagggt gactggaaag ttttctatta tccgtatgcc
    21361 gagatacggc atctgattgg agcgagcacc aatcaggaaa gatcaaaaat ggtaattcaa
    21421 catcataaaa gtatgtatca tttctatcaa aaacattatt cagccaaagc cattttatct
    21481 atcttggtgc tgtgggggat cttccttaga atggggatag tgctattgag ccagatggtt
    21541 gagttaattc tccaaacgaa ttcaaaaaag gaaaaagttt gatagtcctt ttcacgttca
    21601 tcatatcatt gctcataagt tttgccataa ccttttggat tagaaatgtt tgtatcaggc
    21661 ataatctatt ggatcatcca gaccaaagga agatgcatac aaccccgatt cctcgcttgg
    21721 ggggagtggg catatttctg tccattttgg tatcgctctt aattgttttc cttttcaaga
    21781 gttcgctctt ccgtgaaata gaatctcaaa tcctgtttgt gctattaagt gggatggtga
    21841 tatttggctt gggattatat gatgacctta agccggttaa accagaaatc aaactaattt
    21901 ttcaggcaat tgcctccctt attctaatct tagctggctt gaggataaac ctcattcaca
    21961 taccttttta tcgaaccatc aatttgggat ggttcagtta tcccctcacc ttaatttggt
    22021 ttttagtcat cgtcaatgct atgaatctat tggatggtct ggatggtttg gcggctgggg
    22081 tttcagtcat agccgcaggc tcacttttag tagtaggtta tatgctcaaa gtggatatcg
    22141 cccttttgat atccgcaagc gttgccggaa gctgtttggg ttttctaaga tataactatt
    22201 ttccggccag catattcatg ggggattccg gttcactttt tctcggttac ctttttgcct
    22261 tgatggcaat catctgtccc atcaaaagtt acaccagtat ggctcttttc gttccgcttt
    22321 tggctctggg ggtgccgcta attgagataa cgatttcttc ttttaggcga atcataggaa
    22381 agcaaacctt atatctggct gacaaaaggc atatatttca tttcctgata gaaaaaggtt
    22441 tcagccatag agatacagtc tggctatttt atcttttgtc cttgatcttc gctattatgg
    22501 tgatcagcct tctgactcag agccataaaa tcgctttgct cttggtcttt ttattaatat
    22561 tgaccttcgg gacaattttt gtatcttggt ataacaggac gcaagcaaag ctcaaagcgg
    22621 agaagtaaaa gcgatgaatg gaattcactt ggagtttgaa aagcccatct acgagctgga
    22681 aaagaaaatt caagatatga aggattttgc tgacggcgag aatgtggagc ttacacaaga
    22741 gatagagctt ttggagaaaa aattagataa gctaaaaaga gaaacctatt ccaacctgtc
    22801 ccgttggcag agagttcaat tagctcggca tcccaaaaga ccccacagtt tggattatat
    22861 caatctaatg ttgaccgatt ttgtggaatt acacggagat agaaattttg cagatgacaa
    22921 ggcggtggtg gggggggttg ccaagctgga gggtattccg ctcctagtgg tgggtcagca
    22981 aaaagggaag gacacaaaag agaacctctt tcgcaatttc ggcatgtgca atcctgaagg
    23041 ttacagaaag gggttgcgtt tgatgcaatt ggcagctcgc tttggcagac cgatagtgat
    23101 attaatagat actccgggtg catttccggg aataggggcg gaggagagag gccaggcaga
    23161 agccattgcc agaaacttaa gagaaatggc cagactaccc gtgccgataa ttgtgaatat
    23221 cattggggag ggggcaagcg gaggggctct gggttttggg ataggagata agattctaat
    23281 gttagagaat tcctggtatt cggtcatctc acccgaaggg tgcgccgcca ttttatggag
    23341 agatagttct aaagctccac aggcggctga ggctttgaag ctaactgcct gggatctgga
    23401 ggagttgggg gttatagaca agataataga ggagcccctg ggcggtgctc accgtaatta
    23461 tgaggaatcc gcacgtatat taaagaaaga gataaaagct gttttggaag agttgtctaa
    23521 tcttggcccg gaagagctga tagaaagaag aatcgccaag ttcaggcaga tgggttctta
    23581 tgggattgag aaagaggagg aaacctaact ttatgcttga ctggaattca ctttcgattc
    23641 tggtttgtcc taaatgcaaa ggtcagttga aacataggca caaaccggaa ggattagtct
    23701 gtcaggcgtg cagattggat tttgaaatta gagaagatat acctatcctg cttttggagg
    23761 aagcacgtaa attctaaata tcgaaaataa ggaggtatgg taatgaaact ttccaagttc
    23821 tgtgaagaga atttgatatc atttgagctt aaggctaaag ataaggaggg agcgataagg
    23881 gaattagcgg acttggcatc ccgatccagt ttggttaagg ataaggaaga gcttcacaag
    23941 gcagtgattg agagagagaa gttggtcacc accggtgtag gttatggggt tgccttccct
    24001 catgccaaga ccaaggccgt caagggaatc gtcatcgctt tcggcaggtc gaagcagggg
    24061 atcgattttg aagctatgga taagaagccg gtttacctct tctttttgat tgccgcaccc
    24121 gaagatgtga tcggagctca tttgaacgtt atggcgcgac tctcctatct tatgaaaggc
    24181 gaaaagaatc gggatacttt aatgaagata aaaagccctc gagaattaat agagattctg
    24241 gattcagtgg attaattatg cccaagtttg cgcccatcac cctcaaaggt aataaatact
    24301 tctatctctc cattctaata cttctttcgc tcatacttct aattatgcct cattatacta
    24361 agacgataat ctcatattat gtcttcaaat tcagttattc ccctttttat ggactaagcg
    24421 ataaggttgg agagctgaga ggggtttata aaaagaatca agagctggaa aagagagtag
    24481 cagaattaac tttagagaat tatcagctta aagaacagag catagagaac gctacgttca
    24541 gagctttatt gaagttcaaa tcagagagca gctacaaggt gatacccgct aaagtcatag
    24601 cctttgaccc gggcaggaac acaagcaccg ttttagttga tgtgggtggg gaggagggga
    24661 taagaaggaa tatgccggta ataaatatgt atggcttggt gggcaaaact ttggaagtca
    24721 tgcctcatac ttctgcaatc cagcttctct cggaccctaa ttgccgagtg gcggcaattg
    24781 accagagaag cagaatactg ggcatagtta aattgggtcc tggcctagct ctagtgatgg
    24841 ataatgtacc atttggagag gatataaaag ctggggacca gattgtcacc tccgggttag
    24901 gagggatatt tccctcaggg attcaggtgg gcaaagtagc ggaggttgaa gaagccaagc
    24961 acgctgtttt caagaccatt cacctaaaac cagaagttaa tttcaatagt ctggaggagc
    25021 ttttcgttct ggacgtctca caggggtaaa atatgttttt caagaacttg cgatattcca
    25081 aggcagttga tcttgtgatt ttccttttga tgatgctaat ctatcaaagc ataatctcca
    25141 ggggggtgga ggtggctgga atcaccctgg attttgcttt gataattttg gtctacgtcg
    25201 gcttaaccag aggagcagtt ttcggtgcgg tttttggttt tgcctctggc ttgctcttag
    25261 atgtctatac tccaggactt ttggggttgg gcgccctcct gagggggatt ctgggattct
    25321 taataggcaa cttcagaaac aagctcttta tagagactct ctattccaaa ggaggcatga
    25381 tatttatagc tctcttgtgg catgacctct tttactacct ctttgctacc acattccaaa
    25441 taggagctac cacttatatc ctgcttttca attccctacc gtcaggttta tacaccgcag
    25501 taatagggat ggtcgtcttc ggcttagttc aaaagagact cacccaacca ggacaggtta
    25561 caggtttatg aaagatgcaa cttatcctcg tgagaaaaga agtgcaattc tgatttctat
    25621 tctagccatt ttgctttcgt tgctgattat ccgtgttttt ggtcttgagg tgataagggg
    25681 tacatactat cgccagatgt ccgaggagaa cagaatcagg cctgttcctt tgccggctcc
    25741 tcgcggtctt atcgtggata gaaatgggaa agttattgtc tccaaccgtg cctcctatac
    25801 tgtttccata attccctatg aagtgaggaa tctaaaggag gtggtgggaa ggttaagccc
    25861 tcttcttcag atagatgcag atggtttaat gcaaaggatt aaggacaatt ggtccagaag
    25921 gtttgagcca attaccatta aaagagatgt tgatttcaca accatatcta ttctggaaga
    25981 gcaaaatcag gacttccccg gagttatcta ccaggtagtg caaactcgaa aataccctga
    26041 aataagaagc atctcgcacc tgattgggta tgtcagcgag atatcaagtc aagagttagc
    26101 tagcttgccc gctcagagat atagagtcgg ctcatatatt ggcaagaaag gcatagagaa
    26161 gcaatatgat gacctcttga ggggaaaaga tgggatgttg tatctggaag taagcgccat
    26221 cggcaagata ttgggtgagc ttaaggacaa ggagcccgac cctcctgtgg ctggctcaga
    26281 tatcaagtta tccatcaatt ttgacttaca gaagttagct gattccctgt tggaaaaaca
    26341 tagtggcgct accctggttg ccctggatcc aaatagtgga gagattctgg ctctggtgag
    26401 caaacccgga tttgacccta acatctttgc cggggtttta accccggaag agtggaatag
    26461 tttaagccag gaccctaatc atccccttct ggatagagct attcaaggca cctatccccc
    26521 tggctccact ttgaaacttc tgactgcggg tgcggctttg gagacgaaag tgatacagca
    26581 ggaaaactct tttgcgagtt gtgtaggagg ttatcgtttt gggaatagga ttttcaagtg
    26641 ttggcaggaa agagggcatg gtaagttgaa attgcacgaa gcgatggtcc aatcctgcga
    26701 cgtttatttc tatcaattgg gtttgaaggt gggattggaa aattggtgtc attatgctgg
    26761 gctctgcggt ttcggtgaga agacaggtat tgatttaccg gaggaggcta agggtttcgc
    26821 tcccacactt tcctatttta tcaagagaca tggcaaaggg gaatgggtga aaggattggt
    26881 catcaactta gccatagggc aaggtgagat ttcagctaca cctttacaaa tggcaacatt
    26941 ctacgctgga ttagccaata aggggattat ctttaaaccc catcttttaa aacaagctat
    27001 caccccatca ggaaaagtct ttgtcaccaa gacggaacag ctcatcaagt tgcctttctc
    27061 tcctgagact ctggatactt tgaatcaggt tttgattggt gtggtgaatg agccattcgg
    27121 cactggtggt gcatccaggc ttgcggacgt aactgtggct ggcaaaaccg gtactgcgca
    27181 gaatccattt ggtggagacc atgcctggtt tatctgttat gctccggctg aagcccccca
    27241 gatagtcctg gctcttttgg tggagaatgc cggtcacgga ggaacgatag ccgctcctat
    27301 tgccaaaaga atactgcaag ctttttttga aaagaataaa gaactccacc cacagggcgg
    27361 ggttctaaac ccaaatcgag atcccgaatc aagttcggga tgacaagaac cgaatcaagt
    27421 tcgggatgac aaaaaccgta aaaccaaacc ctgttgtcat ctggtctgtt tttctctgtt
    27481 tccaagagaa gcacctttcg tcgccgtcca cgaacacggg gttttcaggt acgatcttga
    27541 taagatctct taatttactt ggaattcggt aaggagaaat gaaacttaaa ttcccgcaag
    27601 aaaactttga tatctggctt ctcgtaaccg ccttgtcttt atctttgatt ggtttgcttt
    27661 tggtctatag cgccagccga aacgtatccc ttccggctgg gtcacatctt ttttctaagc
    27721 aggtggtctg gcttctgctg ggactggtat gtggggcggt cttcttcgtt attcccctta
    27781 aattacatga tttcttttcc gggttctatt acttgatagg tataggtctg cttttaattt
    27841 tagatttcgt ggggaacgaa aagggaggag caatacgttg gtttaatttc ggaagttttg
    27901 cctttcagcc ctctgagttt gtcaagttgg cacttgtttt tattttggct agatacctag
    27961 cctattctca acggtccttt ctgaatttca gatggctttt cactatcttt gccataactt
    28021 tagttcctat ggttctggtc ttgaagcaac cggacttagc cacctccttg attttcatcg
    28081 ccacactagt ggtaatgctc ttttgggctg gagtctcgcc cctttacatc ctgctccttt
    28141 tgtctccttt tatcagtctt gtggcagctt ttcattggat aacttggggt atattctttt
    28201 tctttgtact tctccttttg tatcatttaa ggcccaattt ccttttgagt tcgtctctga
    28261 ttctctttaa ccttttgttt ggtatggtga ctccattaat ttggaatcgc ctgcacgatt
    28321 atcaaaaact gagaatattg gcttttcttg atcccaccag cgatccccaa gggtcaggat
    28381 atcaaatcat tcaatccaag gttgccatcg ggtcaggcgg gttttttgga aaaggctttc
    28441 tacatggctc tcagactaag ctggcttttc ttccagccca gcataccgat ttcatctttt
    28501 cggttttggg ggaggagttt ggtttttggg gtgttttggc cttgctggct ttgttttttg
    28561 ttctccttct aaaagggttt gtcatagctc gcaaagccag aaacagcttt gccagtttcg
    28621 tggctctggg tataagctcg gttttggctt ttcaggtagt ggtcaatatc gggatggcga
    28681 caggcataat gccggtggct ggtttaccct tgccttttat cagctatggt ggcagttcaa
    28741 tgcttctggc ttggatttgc accgggattc ttttgaatat aaattatcac tggtacgaat
    28801 attaatctgt aggagaaact atgcatccgg ttttgttcag attgggtccc ttggccataa
    28861 caagttatag tcttttgttg gtgctggctt ttgttttggg agtctggctc gcaataagaa
    28921 gggccaggaa atccggcgta aaggtagaaa cgatagttga tttagcgatt gtgatttgga
    28981 tttcatccat tgtcggctca aggtttttct atgtcatatt tcatctggaa gagtttaagg
    29041 gaagattctg ggatgtagtt aatcccatcc acccaggcgg agacataggt atagttggtt
    29101 tatctatggt cggaggggtg gttttggcta tactgaccgg actggtctat ctttacttaa
    29161 agagattgga agtgtggaag atagcggata ttgtttcgcc ctcttttatt ttgggtttgg
    29221 ctattgcccg cctgggttgt ttcttgagtg gttgctgttt cggaaaacca accggcagtt
    29281 ttttgggggt cgttttcccc ttggattctc cggctggata tgtctttccc gatgcccacc
    29341 ttctgcccac tcagattttg tcaagtattg gagcggtgat agtactggtg attcttttat
    29401 ttctcgaaag atataagaac tttgaaggat tcaccttctg gcttatggtt atgctctact
    29461 caatcaaccg cttcgttatc gatttcttta gatattacga gcccagcaca acttttaagg
    29521 ttttgcacac agctttttca tccaatcaga ttaccctctt ttttgctttt ttcgtttcac
    29581 tgtttatgtt tgtttggttt gagaaaaggg caagataaaa tcagatatgg ctgtgaacga
    29641 aaagttcaaa ccccttcttg atttgaaaga tgacatctta gatttgatat tcccctaaca
    29701 ctgcatcctc tgtaagaaat atctttcccg caaagaggaa aacgtttgtc agctttgctg
    29761 ggacaatctg attcaacttc cttttccttt ttgtgtggaa tgcaggtctt tactggaaaa
    29821 agacaaagag tgcaagcagt gcggagataa agatgggttg agcttggttt attctttggg
    29881 aagtttcgat gatagctatc aacgtttgat ttacgctttc aagtatggtc agttgctcaa
    29941 tttgggaaag agattgggtt cgactttggg agaaaaaata aaggaggaca aaagattctt
    30001 ggggtgcgat tttttaattc ctgttcccct gcatcctagt cgaaaaagaa aaagggggtt
    30061 taaccagagc gaaattttgg ctttggcaat atcagaaaag attggacttc ccgtcttaaa
    30121 ggacgtttta aagaggaaga aaagaacaaa agatcaaact actttgaatg ccaagcaaag
    30181 agaagaaaac gtgaggggag cttttagtgt aaaaaacgaa gatagaattg ttgacaagca
    30241 aatcatattg gtggatgacg tgatgaccac cggagcgact ttaaaggaat gtgcaagaaa
    30301 cttatttgaa gctggggcaa aagaggtttt gggattgacc gtggcagtgg caggagagta
    30361 gctttagggc ttctgacaac gagggcagaa gtaacttgac ctttgattta taactattct
    30421 tttaatcttc acaccgcatc tcttacaggg tttaccttcc cttccataaa ctttatgcga
    30481 caattggaac cttcccatct tacgagatag gtcagtatat gtcctgatgc tcgaaccgcc
    30541 agttcgcatt gctttcgcta atatctttcc tatgctctta cgcaaacccc ttaatttgac
    30601 tgtattgatt tggtctattt tcttcaaagg atttatccct gcttcaaaca aaacttcatc
    30661 tgcatatata tttccaattc cagccagaac cttctgattc aaaagaacag acttaaccct
    30721 tccttttctt tttctcatta agtccacaaa ttccttttcg gaaatctcca ttggctcggg
    30781 tccgagttta tccaagtcag gacaagtctt gaggtcaaca aatctcattt tgccgaattt
    30841 tcgagggtcg ttatatctaa gctcttttac gtcacccaaa aatctaaaaa tgatgtggtc
    30901 gtgcttgtcc actttcctat ctggacaaag atataagaga tgtccactca tccccaaatg
    30961 gaccagtata gcgttatctt gagacaggca gattacaatg ttcttgcccc ttctttttat
    31021 atctttaact ttttgcccca cggtttggca tataaaactt acactactct gtttaactat
    31081 tcccgggcaa agaaatttca cctcttttat tctcttacct aagatggttt ttcttaagcc
    31141 ccgaacaata gtttcgactt ctggcaattc tggcatattt ttttaaatta taaattattc
    31201 gggcggccac acagttcgac aagctcactg tcctgagttg agtcgaagga agggtcgccc
    31261 ctccaaaaag acagtttaaa atttagaagt atatggggct aacccagaag gcgttttaat
    31321 ttacataaac tttctttgcc tgtcattgcg aggagggcgt agcccgacgt ggcaatctct
    31381 tccagcacca taagttataa aaccgagatc gccacgctct cccgatagaa tcgggatcgc
    31441 tcgcgatgac aaggagcttt tgggtgagcc ctataagaat gtctataatt ttaacttaat
    31501 tctaacatcc gttcaattga ttttcgggct tttgcggcaa tatcctgggg caccttaatt
    31561 tcatagagca tatcttccaa agaccaaagg atcttctcta agttggtcaa tttcatattg
    31621 gagcaatcag ccagaaggct ggctggatag aattttttat ttggctcttt tttctttaaa
    31681 gcatgtaaga tcccgatttc agttccaatg atgaattcct cggagcttga ctcggagaca
    31741 tatcttataa ttccgctggt agagagaacc ttgtctgcca tagcaatcac gtccggcgtg
    31801 cattccggat gaaccaagac ttccgcctta ggatggagat tcttattttt taagatatct
    31861 tcagccagaa tccttcgatg agtgggacaa tatccttccc ataaaatcaa atccctttta
    31921 agttgagagg cgacataact tcccagatat ttgtccggta taaacaaaac ctctctatct
    31981 ttgggaatat gctccacgat cttgaccgca ttagctgatg tgcaacaata gtcgctttcc
    32041 gccttaatct gggcagtgga gttgatgtaa caaatcacaa ctgcatgcgg atgccttctc
    32101 ttcgtttctc ttaattgtct tagggttatc atatttgcca tagcgcatcc ggcatttaaa
    32161 tccggcatca aaaccaactt attcgggcag agcaagcttg cggtctctgc cataaagtga
    32221 actccgcaaa agattataac ttcagcttcg gttttgctcg cagtctggct taaacccaga
    32281 gagtcgccaa cataatccgc aatatcctgc acttcgccca gctgatagtt gtgagccagg
    32341 attatggctt ttcttttttc ctttaactcc aatattcttt ttgtaatatt gttattttcc
    32401 atattggatt gtttttctta tttctcttca gatacagttt tatttaagtc atccataagc
    32461 ttgtccacat ccactccgtg ggctaaagct ccctgctcaa ttgattcctc cgccgcaata
    32521 tggcagccga agcaatgcat accatatctg aaaaaagtct ctgcggtttt gggatatttg
    32581 ctcaaaacct caccgaaggt catctctttg attagtttct gttccatctc tcctccttgg
    32641 tttttccacc cctcttatct aatacgatat ttaatttata tttttgatat gtcaagagtt
    32701 aaattttggt ctctgaagaa gtgtagccga caggtcgggc aaggtgccct gtaccagcac
    32761 ggttcagggc ttgcccgacc actctgccgt actttttcag cggtctcaaa ttaattcctc
    32821 caccaattca gctatatctt ttacgcagag cttttcctcc agggtttttt ccttaacccc
    32881 atcctctaac atagtcaagc agtaaggaca ggcagtggat atcacttcgc ttccagttga
    32941 attcaactgc tcaatcctca tctggtttat tctttttcct aaattctcct ccatccacat
    33001 ccttccgcct cccgctccac aacaaaaact ctttgattta gataacttaa cctcattgag
    33061 tttcttttga ctcagtcttg ttataatctc tcggggagca tcgtaaatac cattataacg
    33121 ccccaaataa caggagtcgt ggtaagttat tgatttttca ttttgtgatt tcaatttgat
    33181 tttattatcc ttgatcaaat tccatataag ctccgtatga tgtatcacct cgaaatctcc
    33241 accgaattct cgatattcgt tcttgaaggc gttatagccg tgcgggcaag ttaccagtat
    33301 tcttcttacc ccgtatttct tcaaaatttc aatattcccc cgcatcaaga tttgggctaa
    33361 atattcattt cctattcttc tggccgtttc accacagcac atctcctcat ttcccaaaat
    33421 ggcgaagttc acttttgccg ttttcaagat tttgaccaga gcgcgcgaga cttttttatt
    33481 tctgtcatcg aacgagccag agcaacctac ccaataaagg atatcaaaat tcttatcttc
    33541 cctggcaagt tttattccta aatcctttgc ccaatcagct ctggcttgat aaccgaatgc
    33601 ccaagggtta taattggtct ccatattctt gaatagattt ttcaattcgt taggaaatct
    33661 gctctgcatt aagaccagat gacgacgcat atccacaatt ttgtcaatat gttcaattaa
    33721 aacaggacaa gcctggtgac aggcataaca ggtggtgcaa gaccaaattt catcctcttt
    33781 aaccgaacct ccaatcaaag ccacagaggg agtacctttc ttatctttgc ccaaaagttc
    33841 tctggttctt gcttgaagat ttgccttgat atcttgattt acctttttgg gagaaagtgg
    33901 tttctggctc aaataagcag gacaattatc ctgacatctt ccacaacgag tgcaggcgtc
    33961 agtgtccagg agttgtttcc agttaaactg ttcaacccta tctactccaa aagtctcctg
    34021 attttcaata tccaagatag ccggtatccg acctttgggt tcaaatgagc gaaaaaagat
    34081 atttaaagaa gaactcagga tatgcaataa tttggagtag gtgatatagc tgatgaaaaa
    34141 caaagccaga gccagatgaa tccaccaaga gattagatgg agaattctta tggagttagg
    34201 atttaagttc cagaaaagtt tagacaagtt ccatcccaca aatgaccaaa cctcgaactg
    34261 cggcttggta gcagagattc tcagggcttc attcaaaaag ccaccagtgg caattgccaa
    34321 caacaaaatc aagactacgg catcgtccaa gatgttatct aatcgcttcg gttttaaaat
    34381 gtatctcctg aaaagagcca tgcctattcc gattatcaga agaagcccga agagatctaa
    34441 gattagagaa aaatacagat aaaagtaaga atggaaaaag aagatgccaa ataggggaga
    34501 ggcgatgtca tcctgaaaca tcaccaccgc ggtgcctaag aaaagaacca caaatcccca
    34561 gaagatgaaa aaatgcatta aaccaggata taaatccctg aagaacctct ggtggaaaaa
    34621 gccaaaggat acaaaagata agatcctttt tcctattttg tcgaattttt tttccggctt
    34681 acctattctc cagagcttaa tcttcttgta aattccccaa atgaggaata agaggggtat
    34741 tattgccagg atatagataa taattcttat tcctgctcca acgttccaga aaatctctcg
    34801 ggttatctcc atacgtttaa ttaatttcct gcctttgtct ttttaatctc ctgagctaaa
    34861 agaggaacta tctgaaagag gtctcccaca atgccgtaat tcgctttctg aaaaatgggc
    34921 gcctcggggt ccttatttat ggcgattata cattttgaag tttgcattcc agctaaatgc
    34981 tgaatggcac cggatatgcc acaggcaata taaacttccg gattgaccac tttgccggtt
    35041 tggccaacct gcatatcgtg gggtgcaaag ccggaatcaa ctgcagctct cgaagctcca
    35101 acagttgcac cttcaccaaa ggagcaagcc aactcctcca atatcttgaa gttttcactg
    35161 cctttcattg cccttccgcc agaaacgata acctttgcct cagtcaaatc gaccttctct
    35221 ccagccgatt ttactatctc ttttactttc gcccttatcc cttcagttga gattctaact
    35281 tcggatttta ttatctccgt tcgtcttgat tcatccggag aggccattac aaggacattt
    35341 ggtctaatgg atgccatctg aggtgagcta tcaataaaaa catcaactat cacctttcca
    35401 gcatagattg gccttttgag tagaagattt ccatttgagt ctatatccaa agtgatgcag
    35461 tccgaagcta agccagtccc gagtttagcc gcaactcgag gaagaaggtc tttacccata
    35521 gcagtgctag gtcctaataa gatttttggt ttatgctctt tcacaaaatc cgcaatgacc
    35581 tgactatacc cttcattcga ataattcttc aaaatctcgc tatcacataa aaagactttg
    35641 tccgtcccat aataacctag cacattagcc aggcattcaa ccttatcacc caaaagaatc
    35701 gcacccagtt gaaccttaag tttatccgct attttgcggg cacagcttaa aatctcaaaa
    35761 ctcacctttt ttagttttcc atctctttgc tcagctatca cccagacttc atttgccatc
    35821 tttttctcca cttaaaccca gattttgcag cagcaaaatc tgcccaaagg gccgctacgt
    35881 tccgctgtgg cggaactaac ggggttaacc ccgcctttag gcggccttcg ggaaatttgc
    35941 ggttaaatta tcttttcctc ttggtgcagg atttttgcca gctcgactgc tacctcaatt
    36001 gcttcacctt gaattatctt tcctgcttcc cttggaggag gaaagccgaa attgattagt
    36061 ttgattttgt ttcttagctc gtcaactggt atgcccaaat cagataaagt caggttttta
    36121 atttcctttt tcttggcttt cataattccc ggcaaagagg cgtagcgcgg ctcatttaat
    36181 cccttttggg cagcaattat cacaggtaag ctcgtctcca ctatctctat tcctccagcc
    36241 tcaatctccc ttttggcaat cgctttggtt ttatcatctg atatttccaa tcctactact
    36301 actgaaacct gcggtatgtt cagtatctca gccaatacag agaaaacctg cgagttgtcg
    36361 taatcaactc cctgctttcc agtcaggatg atatcaaagg gaatgttctt caaagccatg
    36421 ctgagagctt tggccgtgga ataactatcc agtttttcaa aggcagggtc gcatagatga
    36481 actgccttgt ccgctcccat agcaagggca gtgcgaatcg attcagttac cctttccggt
    36541 cccaaacaaa gtatggtaac ttctcccccg aatttttcct taatccttaa gctctcctca
    36601 atcgcatatt catcataagg gttgattaca aatttcaccc cctcctctga aatgccgtct
    36661 tttccctgat ttatctgaat cttagtttca gtatccggca cctgtttaag acagactaca
    36721 atgttcattt agtcctcctt tacatactca atcgggtaag taatggctgc gccattactt
    36781 ctgctgtctt ttatcttttc gggaacgctg caactgttcc ctacccgctt ctttatacta
    36841 agctcgaggt tatactaaaa acaaaatagt aaatcgcaaa actgttgtca aacgaaaaac
    36901 ttctcaaatc tatttggcaa ttttgatttt agatgttata atttaaaaat atggcaaaaa
    36961 tattagagga actcaatcct cctcaaaagg aagcggtttt gcataccgaa ggacccttgt
    37021 tggtcttagc tggtgcaggc agtggcaaga ccagagtctt gacctatcga atcgcctatt
    37081 tggtgggaga gaaaaaagta aatccctaca acatcctagc tatcaccttt accaataaag
    37141 ccgctcgtga gatgaaaagc agggtggata tgcttttagg aagaggaagc gattttctct
    37201 gggtgagcac ctttcattcc ctttgctcgc gcatattaag aaaagaatgt caggtgttgg
    37261 aatataagaa acatttttca atttacgatg aagctgacca gatctcttta atcaagaaat
    37321 gtgaggagga attaggcatt cccataaagg agttttcgcc ggcagctgtt ttgaatagaa
    37381 tatcacaagc caaaaataat cttattggct ggcaggattt cgcttcaatc gctcaaaacc
    37441 attttgaaca aaaggtttct aagatttatc ctctttacca gaagaaatta aaggagaatg
    37501 acgctttcga cttcgatgac ctgattatga agactgtgga gatatttgaa aatcatcctc
    37561 aagtcttaga aaattatcag gacagattta agtatatctt agtggatgaa tatcaggaca
    37621 cgaatcacgc tcaatatgcc ttaattaagc acctttcctc caaatacaag aatttatgcg
    37681 tggtgggaga tgatgaccag tccatctatg cctggagagg agctgatttg aacaacatct
    37741 tggattttga gaaggatttt cccgatacca aagtggtgcg tctggagcaa aactatcgct
    37801 ccacccagat catcctggat gcagccatat ccgtagtgaa aaataacgtc agcagaaaag
    37861 ggaaggttct gtggaccgag aaaaaaggag gggagaagtt ggtagtatcc tatctggaag
    37921 atgataggat ggaagcaaaa ggtgtggtgc agaggatgga tcatctggta gctaactttg
    37981 gctacggccg ctcagatttt gtggtgttat atcgcaccaa tgcccaatcc cgaataatcg
    38041 agcaggagct aagggataag ggcattccct acaccatcgt gggtggagtc aagttctatg
    38101 agagaaaaga ggtgaaggat gtgttggctt atttgagagt gttggttaat gcagatgatc
    38161 agctcagctt aaagaggata atcaatgttc ctcacagggg aataggtcag aagaccatgg
    38221 ccagaatcga agaggaggct tttaaaaaga gaaagaattt acttgagact ttaaaacata
    38281 tacgagatgt gcaaggtata tcggatagaa tgaaaaagga aataaactcc tttgctaatc
    38341 tcttaaatga attgaatcaa ctgaagaaga gtctgaaagt tgaccagctg accgaagagg
    38401 tgattcaaag gagtggttat ttagatagat taaaagagga gaggacaatt gaggcccaga
    38461 cccgcgcgga gaacgtggag gagttagttg cagcaactaa ggagtttgtg gagagggtag
    38521 aaaatccgag tgtggagagt tttatggagg aagtctcact tttgactgat attgaccagt
    38581 gggatgatac tcaggatgta attaccctga tgaccttgca tgcggccaag ggattggaat
    38641 tcccggcggt tttcataaca ggtttggagg aaggcctttt ccctctttcc cgttcactgg
    38701 aaaagcagga agacttagag gaagaaagaa gattatttca cgttggattg actcgagcca
    38761 aagagagaat attcttatct tatgttaatt tcagaaggcg ttttgaaggg atgcttaacc
    38821 tgccttcgag gtttatagat gagattccag aggagcttta cgatttcgaa gatctctgtc
    38881 atcgaggaca acacaaattt tatcccaata tcaaaagaga atatgaggac tgggaaatag
    38941 cagatgatat tctaaaagtg ggaacctggg tattgcattc caattggggc aagggaagaa
    39001 taatagaaaa gcaggggtac ggtgaagact cgaaacttac tgtgcttttt tcctacggag
    39061 ttaagaagaa gcttttagcc aaatatgcca atcttgagat tttacagaag taggttttta
    39121 atgtagcgga ggtctttaga cctccatcta ccccaaatag atgctataga aagctgaagc
    39181 tttccgctac ataaaatatc agggacagaa cattgttctg tccctacaaa ttctcaaaaa
    39241 cccaaaactg atttatggta ggtatagata cttaggccta cctctgcgtt ctgtttaaag
    39301 taggcgacat tcgcgaatgt cgcctacaaa gctttctgct aatgcttgtc aataaaaatg
    39361 gaaacctaaa ggtttccgct acataatgcg tgattatttt ctaagtttct tagctatttc
    39421 cgcaactctc ttgcccaatg ccttagctaa ctccagatca tcttcagttg gcgatttatc
    39481 agcttttggc ccagagacgg atgacgctcc ataaggtgag cccccgccag ttgtactgaa
    39541 aagcctttgt tctgaataag gtattccaac gattatcatc ccgtggtgca ataaggtcag
    39601 catcatggtg atcaaagttg tctcttgtcc tccgtgaggc gtagaagtgc tgcagaataa
    39661 tccagccggc tttccgataa gctgcccttt tgcccacaag ggtccggtag tatcaagata
    39721 ttgccttaac tgggcacaca tatttccata acgggtggga ctaccaaaaa ggactgcatc
    39781 cgcttcaatc aaatcctgaa gcgtagcaat ggggatatcc ttttgcatct ccttggcttt
    39841 tatcacctga ggaatcttgg agtaaatctc cttgggtata agctcaccaa cctgccttag
    39901 atttacctct gccccctcaa ctgatttggc gccttctgcc actgcttggg ctaatttgaa
    39961 agtgtttcca tacatactat aatagaccac caggattttc atacttgttc acctcgctta
    40021 gagtatcctg ttgtattttt tgactatggg cgggttttct cgggcgtggg cgattccaca
    40081 aaatttctcc cagagcgtgt ttatctgtgg gtccgcatcc acctgctgtc tgaccttttg
    40141 gtaatgctct tcgctttcga agtaaatcac ctccatgcaa aatccggaag agcccttctc
    40201 ctcgtagacc tcataaccag aacatccata cctggcgtat aaggattttg cctgtttctg
    40261 cacttccaga aattcgtctt tccttccaaa aggaaccctg taagtatagg ttatgatgaa
    40321 cataaaaccc ccttaaaatg aaaatcttat tcctacttga tttatcggtt tgttcacgaa
    40381 agttccgtaa attctttgat agtttagttt gaaattctta aacacgggtt tcaccaagcc
    40441 catttccata taataatcat ccccggaatc ataccaatta taatctaaac aaatctccaa
    40501 gtcaaaccaa aagtatagct tgaagctgtt gactgtaact atctgcaaag tctgaccagc
    40561 atcatccagt tcatccagaa agttcttata caaagagaga tcatcggtga ttttgttgaa
    40621 acccaaaggc tgagctaata taaaaacagg aaaaaaggaa aatagaaaaa ggaaaatgaa
    40681 aaacttggat ttcaaagaac cctcctttgt cataaatcat atttgaccca cgttgcctgc
    40741 ccggccaggg agggcaacgt gggtatacta tttttgcgta ggggtggagt ttatctccac
    40801 cctgcgcctt cgggcgaaga cgagcttcgc ccctacgcgt cgaagatttt ttggagtgca
    40861 ttgaccatgt caatgctgca tcgtcacgga cgatgcactc catagataat acctttcaca
    40921 tccccaaagt tctatccaaa cttctatact gaatcgcttc gctgatatgc tggggtaaga
    40981 tttctttttg gttttccaaa tcggcaatgg ttcgggcaac ttttatgatt ctatcataag
    41041 ctcgtgcaga taagcccaaa cgggtgatag ccattctcaa aagttctttg gagctttcgt
    41101 caatcgggca atattttctt atatccttag aatccatatg cgcattgcag aagatatgct
    41161 tttctttctt aaatctctct aattgagttt tccttgcctt attaactctc tctcgaataa
    41221 gggttgaatt ctcgccagtc ggctcaccgg aaagctcctt aaatttgact gcgggaactt
    41281 cgatatgaat atcgatcctg tccagaagtg gcccggagat tcggctgaca tatctttgaa
    41341 tttgcaaagg agagcaggag caatcgtgga tcaaatctcc atgatagccg caagggcagg
    41401 gattcattgc gccaactaac ataaacttgg ctgggtaagt taaggacatc aaagctctag
    41461 aaatagtgac tctcccatct tccatcggct gtcttaatac ctctaaaaca tccttgtgaa
    41521 attccggcat ctcatctaaa aacaagactc cgtgatgagc taaactcacc tcacctggtc
    41581 ggggaatctt gcctccacca accagtccgg catcgcttat cgtatgatgt ggagagcgga
    41641 agggacgtgt agctatcaag gcggcatttg agggtaaaaa tccagccact gaatgaatct
    41701 tggttgtctc taaagcttcg tccagagtca gattgggcaa gatggtgggt aatcttctcg
    41761 caagcatagt ctttccagag ccaggggggc caagtaagat tatattgtgt cctcctgcag
    41821 ctgcaacctc caatgccctt ttggtgtgct cctgaccttt gacgtctgaa aaatctaact
    41881 tgtaatctag cgcctcctca aaaacttgat ttacatcaat ggtgaatggt ttggtagaaa
    41941 gttcattttc tagaaaagag acggcctcct tcagattgct taccggataa atctccacgt
    42001 cctttgccat ggccgcttcc ttagcattct ccttgggcaa aatcattccc ttgatgttgt
    42061 tcttacgggc tgaaagtgcc ataggcaaag caccgtgaat gggccttact gcaccgtcta
    42121 acgagagttc gcctaagatt agatagtcct caaagttttc ttttgagact tgcccggtgg
    42181 ctgagagtat gccaacagct attgggaggt cgaaagagga accctctttt tttatatccg
    42241 ccggcgctaa gtttacggtg attttcttat tgggaaaagt aaatccggag ttcttaatgg
    42301 cagattgaac tctttcccga ctctccttta ctgcattatc aggtaatcca accgtagtga
    42361 aagagggaag ctgaggtgag atgtccgcct caacttggac aacataggca tctatgccta
    42421 agactgcact ggaaagtatt ttggagagca tattttatga attaaccgaa tcctttgcag
    42481 gcgacattcg ccaatgtcgc gtagattttt tagaagtgtc aggagtagct cctgacacaa
    42541 tgcgaaacga agttcaaggt ccctgctact aaaatctggt tttcagaatt gatgtgccgt
    42601 gctttctttc cttcttggtt aggccgtttt tttcctctcc ccatggggga gaggattaag
    42661 gtgagggggt aagtttccct ttttctatat tctcttgaat tactcggcaa actccctcaa
    42721 tattcttcag tatctcgcta tcactgaatc tcaatatccg tacccccaat tgcgacaact
    42781 cctttgttct taattcgtct tcacttctgt ggtcatcttc gtaatgctgt ccgccatccg
    42841 actcaatgcc gagcttacat ttgggagagt agaaatccaa tatatatctg ccgatggaaa
    42901 attgcctcct gaactttgcg ctagccagct gacgatttcg caatattgac caaagtttcc
    42961 tttcggtatc tgtttggttt tttcgcaggt ttcgtgatct ttcaacattc tttcgtttca
    43021 tctcactctc tgagatttgt cttccccctc accctatccc tctccccaaa ggggagaggg
    43081 aactttgttt ctcgaggtca tgggaccccc agattaccta ttatctgttc ttctctttcc
    43141 acttcaccac atgcacacaa tattttccgc cgctggggat ggattcggag ataacatatt
    43201 ccaaatcttt gttaagctct tccaccaaag tcctttccat agtggtgtga gcttggcaga
    43261 ttttggggtc atcaaaagga taggggcact ggtaaagctt aattaccact ttatcatctg
    43321 agaactccga gacctctcct tcgcagtcta tcattgagaa aattctcaga agataaagcc
    43381 ctacctcctg gggagttgga tttcccattt tctttttggc gaattcagcc acttgcttgc
    43441 cataagtgtg gctgacttcc tgaattaaat ccagtccagc ttcgccgaat tttttgtaaa
    43501 gacctttgta gatggtattt attctgctaa gaagcgcttt ggtggaagtg cggtattttt
    43561 tatcaagtgg tatttcgggt aattctagtc cctttggagt tttgagcata aaccctttcc
    43621 gtggctggaa aagtccccta aactaccttg tagacagtat cattatgacg gactgcacca
    43681 ttgatcttga ttccgatgga aattccagct ttggcttctt gcacctgctc atgttcaatt
    43741 tgcatagatt ccaccttctg ggtcaggtcg gtggtgtgcc cgtggatgtg gatagtgtca
    43801 cccactttta gaatgccgtg agtgatatca attgcggcaa catctatctt tttgaaatac
    43861 cccgcaatct tacctacgat ttcctctggc ataatccctc ctggtttatg ctttaggttt
    43921 gcggctatct ttgattgtta aaataaaatt ttattttaaa tctccggaaa tggagatacg
    43981 atgcactccg ccgaggtcag cataagatga ataagcgtaa tcgaatctat agactttcca
    44041 gttcacgccc aagccaaagg agaatccagc caaattgtct ttgtccgaac cggtcttgta
    44101 gtttttacca aaagagctcc atcccattct caagaaggcc ggcttaaaag aggaaagctc
    44161 tgcgccaaga ctgaaataga tatcattgtc aaatggttta gccccatcca ctgctactaa
    44221 gagtggcaat ccatgaacat agtgcgacac tccgacttta atttcagctg gcagggattc
    44281 tttatgctct ttagaaaggc cattaagttg ataccccaga ttctgaacca ttactcccac
    44341 ttgaatcttc tttttttctg acttatgaaa agctccaata tccagagcta aagcatcaga
    44401 agtgtaatta tctatcttct ccaagatgaa tttggcattt acacccaaac tgattagagg
    44461 atgaaccttg cgggcatagg tggctatgag ggcgaagtca gccgctccga aggtgcctaa
    44521 gttttcgcca tattggtctg ttttatcgaa ggagccataa ttgaaatagt tgattgaaat
    44581 tcccagtcga gttttttcat cgtagggatg gatgtaacca ataaagccgg attgaatgtc
    44641 agttaaatag ttattgtagc tggtggtgaa acttcggtta gggagttggg ttaagccggc
    44701 tgggttaaaa tagatggctg attggtcatc tgccaatccc acaaaagcac cacccattgc
    44761 tactgccctt gcccccactc cgattttcaa gaaagaatag gcagttgtgc cagcattgga
    44821 attgattccg gcgtaggcat aagatatcag aaagacacct gttaacagag tcaaaattgc
    44881 tattttggtt tttctcatat tttctcctta tttcatattg acaaagctaa taaaatatgc
    44941 tcaaaagtca accgttttat taccaggatt tgactgtgcg gtttatgata tcctcaacca
    45001 gtttctcaac cgccctttct tttccctggc tttcctcctc tgtcaccgaa tagacccccc
    45061 aacccttcat attctcttcc cataagttct tctttttgtt cttatcttcc agccagacct
    45121 tgacgtagat ctccgagata tactccttta tgttttcctg ctcatcataa gtataggccc
    45181 tacgttgata tttggtgata gttccgtaca gaatggcatc tgcctctttc tcgtttagaa
    45241 cttttaagac gtgattctgc acaaaaccat ccaccacttt ttcagttata ctttcgctca
    45301 agccatattc cgcggtctga ttctcaaaag gtggtatggc aatggagttc actccgggaa
    45361 gggaggtgcc gtgaaaggag taaggcccac agctgatagc tagtagtaaa accggaaata
    45421 agacatattt aattgttttc attttcctct ccacatttca aaatttactt ctcctgtatg
    45481 ctctattacg aggttcctcc ttttgtacgc tattgtcatt cccgccccgc atttctgcgg
    45541 ggtaaactcc agcgggaatc cagaaacaga tgaaaccgaa actccagcaa taacaaaaac
    45601 tggattccgc cctaactact gggttttcaa catcaagtgc ggaatgacgt actgtgtgtt
    45661 tccgtaggga tgggaattta tggttcgact ttgctcacca tcctgagcct gtcgaaggat
    45721 cccatcccgc gtaggcgaca ttcgcgaatg tcgcctacaa tagattttat gtaggggttg
    45781 gatttatctt gcctgtggcg ggatttatcc gacctaaccc ccagcgggct tgatgaatca
    45841 agcccctaca aattcaaaac tttaccattc tttatgattt tttcgaccag atttatccca
    45901 taatgatatg gaatctcttt atagttcttt acctcccata taaccaaatc cgcttttttg
    45961 ccaggctcta tgcttccaat ttgattttcc atattcaaag cataagccgg atttatggtt
    46021 gaggcaacaa ttgcctcagc tggggtcagt ttcatcataa ggcaggataa gctcatcatc
    46081 ataggcattg attcactcat tgagcttccg gggttgaagt cagttgagat agcaatgggc
    46141 aagttatttt ctatcatctt tctggcaggg gcgtattttt tcaagcccaa gccaaaggtt
    46201 gctccgggaa gaaggactcc aatcacacct ctttttttca aaagctttat atcggttaga
    46261 gtaacatttt ccagatggtc agcacagata gcaccaattt gtgatgccaa tttcgctcct
    46321 ccaaaatggg agagttgctc tgcgtgaagc tttggcacaa gaccatattt ttttgcagcc
    46381 agaagtatct tttttgaatc tttagtatcg aatacccctt tttcgcagaa gatatcgcag
    46441 aattttgcca atcttctttt tgctaccaga tgtatcattt cctctatgag aagcctaata
    46501 taaccttctt tgttatcctt atattcctct ggaatctcgt gcgcccccag aaaagtcggg
    46561 actaaatcga ttggatgaat tctattcagt tgccttatta cttccaatat ctttatttca
    46621 tctttcaaag aaagcccgta tccgcttttt gcttcgatag tagttgttcc gtaagataag
    46681 aatctatcca atctcggaat ggcaagtttt atcaattctt ctttgcttaa ttttctcact
    46741 tttcttacag acgattttat ccctccacca gctcgggcga tttcctcgta agtttttcct
    46801 tttatcctaa gctcgaattc ctcctctctg gtattagcaa agattggatg ggtgtggcaa
    46861 tcgaccaaac ccggagttac aactctattt ttagcatcta cgatttttgt atttttcccg
    46921 actttaattt tggataacac tttatctgtt ttcccgagtg agatgattct atttcccttg
    46981 atggtaactg ttccgttttc aataatgccc aattcctgca tattttttcc ggcgcgggga
    47041 cctcttttgg aagaacggag agttagaagc tgtccgacgt tttttatgat taaatcagct
    47101 atcatatttc agatgttttc agattgcttc gtcgtcctgt cccgcctgtg gcgggagact
    47161 cctcgcaatg acagcacaaa atgaatgtca ttgcgagtcc ccgcaggggg cgaagcaatc
    47221 tattaaatta ttgggttttt caatccaata gtaattctct gtcaggtcag cctgcccgtt
    47281 tctccccgct caacccaatg gccagggggg acctgacgag cacagaacgt cattcccgcc
    47341 cccgtagggg atagactcca gcggtatgag ccagaacggt tcataccagc gggaatctct
    47401 atgcctttgt aaaacaaatg gattcctgc
//
LOCUS       DASWLN010000039         4715 bp    DNA     linear   ENV 20-FEB-2024
DEFINITION  MAG TPA_asm: Candidatus Zixiibacteriota bacterium isolate
            SMAG_U2949 k141_548464, whole genome shotgun sequence.
ACCESSION   DASWLN010000039 DASWLN010000000
VERSION     DASWLN010000039.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37960594
            Sequence Read Archive: SRR10376091
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Candidatus Zixiibacteriota bacterium (soil metagenome)
  ORGANISM  Candidatus Zixiibacteriota bacterium
            Bacteria.
REFERENCE   1  (bases 1 to 4715)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 4715)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (26-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/12/2024 08:28:26
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 1,440
            CDSs (total)                      :: 1,416
            Genes (coding)                    :: 1,412
            CDSs (with protein)               :: 1,412
            Genes (RNA)                       :: 24
            rRNAs                             :: 1 (16S)
            partial rRNAs                     :: 1 (16S)
            tRNAs                             :: 21
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 4
            CDSs (without protein)            :: 4
            Pseudo Genes (ambiguous residues) :: 0 of 4
            Pseudo Genes (frameshifted)       :: 0 of 4
            Pseudo Genes (incomplete)         :: 3 of 4
            Pseudo Genes (internal stop)      :: 1 of 4
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..4715
                     /organism="Candidatus Zixiibacteriota bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_548464"
                     /isolate="SMAG_U2949"
                     /isolation_source="soil"
                     /db_xref="taxon:2053527"
                     /environmental_sample
                     /country="USA: Oregon"
                     /lat_lon="42.99 N 123.63 W"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            complement(113..1585)
                     /locus_tag="VGB16_01570"
     CDS             complement(113..1585)
                     /locus_tag="VGB16_01570"
                     /inference="COORDINATES: protein motif:HMM:NF015894.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="FapA family protein"
                     /protein_id="HEX9916401.1"
                     /translation="MELTNNSSAFATSISKAKDKKNGVVFELFLSPNEMKAYLLIQPS
                     AEGYTKPTTEEVESYLKEQGICFGLDETAIQEILEQKVYNQRVEVAHGEESVLGEDAI
                     LEFLFNAMPGEDFKIGRDGRMAFKEHGIIQLVKAGQILVKKIPAKPGEFGTTVTGKKT
                     LVRAPKDIRLPVGLNSMPDPNQPLDLISRIEGDIIFKNQRVHIQPLRILKGNVDYTIG
                     NVNYVGSLRVLGSIKKGFSISVDGNLEVDGSVEDARLMVGGNVLIKGGFSGSGKGDLD
                     CRGKVIVKYIKNQLVISAEDILVGGEAAGATLLANNNLKVVGPKGVIVGGDCAAGKVI
                     EAGILGDKEGTSTKIRVAYDRTLMEEYKQIEYELEKLSQDTQRVKGVLYTFEKMQMDG
                     ELSDKDQMVVRKLKDVESNIGPQMEKLKTRRMELEKEINKNLDGKVIVKGKAYPGVEL
                     YFGILKYEITSEMGPTVFEIEEGEIRAKEYKSNQKRGRNG"
     gene            complement(1595..1993)
                     /locus_tag="VGB16_01575"
     CDS             complement(1595..1993)
                     /locus_tag="VGB16_01575"
                     /inference="COORDINATES: protein motif:HMM:NF012245.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEX9916402.1"
                     /translation="MTIKGKTARVEDILASVERFAEEAKLVSLDLAGASARAREANCI
                     PYKVSEEIMDLVDKSNSAANQILEMIRMVRMEMGEVYYLTEDGEVKLNKNRELVGNLE
                     VNLKKILERSDRVLETVNKLRTLSLDNLVV"
     gene            complement(2002..2373)
                     /locus_tag="VGB16_01580"
     CDS             complement(2002..2373)
                     /locus_tag="VGB16_01580"
                     /inference="COORDINATES: protein motif:HMM:NF012301.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_process: GO:0000160 - phosphorelay signal transduction
                     system [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="response regulator"
                     /protein_id="HEX9916403.1"
                     /translation="MKTVLLVDDEEHILKLYQKVFSDDGFQVKLAQSGEQAIQMAKEG
                     DIDLVILDIKMEGISGLETLAELRKSKRNMPIILNSAYSTYKSDFQSWLADAYLVKSA
                     NVDELRLKVRELLEVKVPVER"
     gene            complement(2374..4128)
                     /locus_tag="VGB16_01585"
     CDS             complement(2374..4128)
                     /locus_tag="VGB16_01585"
                     /inference="COORDINATES: protein motif:HMM:TIGR00229.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ATP-binding protein"
                     /protein_id="HEX9916404.1"
                     /translation="MIKLEKTVPLDTLNALLDKIPLGVLVCDASGVITHANPHLPKDQ
                     SYSDPNELLGRNIFDLPSLAKVSSQMRECLALGKEFVLEGLKYMRQDGGEAYLRLKSF
                     PVLSDGKTMGSLSYVEDITEGIRLKEELENGIKRLEIMSELGKSLKSTHKLEDVLRIV
                     MIAFTAREGLAFNRVFLLLLNQDETYLEGKMAMGPANQEEAGRIWSYLSQTKPSFRKL
                     LESHDEHFEERDAWVNHLVKKIRIPIQDKENLLVRAMDGQSCEIVNCSIEERGSGKSL
                     CELLGTSSFVAVPLASKGKSIGIILADNLISGKSIEREKVEFLEVLAYHASAAIESAK
                     LYEKLAHQVAELEKANQRLKEGSERLVRVEKLSVMGEITSQVAHELRNPLAIIGGFAN
                     SILKKLDPNDPNYEYLKIISQESQRMENILDNVLNFTRPERAHWENTDLNEIIEQTLD
                     MLGKEIEDGKIWAKRNYCSPLPLILVNPDQIRHGLVNIFRNAIAAMPQGGTLSIGTSL
                     TNASVKMSVADTGCGIPRERLDNVFKAFYTTKPDSNGLGLTVASEIIKNHNGFIGVES
                     EEGKGTTFYIEFPLKKEE"
     gene            complement(4134..>4715)
                     /locus_tag="VGB16_01590"
     CDS             complement(4134..>4715)
                     /locus_tag="VGB16_01590"
                     /inference="COORDINATES: protein motif:HMM:NF024583.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005515 - protein binding [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="GAF domain-containing protein"
                     /protein_id="HEX9916405.1"
                     /translation="AKEKRPIFISHLHRKSHNSKNSIHSFVSIPLLIKNELVGVLNLS
                     HIRDDAYTKSDLDFLTIFTGQVSIFIERMLYQTELEKKNKELEQAQNLLKETRERSVD
                     NEKLSAISQVATSINHEINNPLAVIAGNTQYLLMTMKNVSPNVKKRLKAIDNEVNQIS
                     QVTRKLLKIKKLVVEDYIDGHKDKMLNLDESTK"
BASE COUNT         1161 a         1289 c          813 g         1452 t
ORIGIN      
        1 cctgacccaa gcagtgtcaa caccctcagt aatgacatgg ctcttctgac ctttatcaca
       61 acttaaaaca caaataattg ctaaaagcag aatcggcaga accaagaatc tcttacccat
      121 ttctacccct tttttgatta cttttgtatt ctttcgccct tatctctcct tcttctatct
      181 caaaaaccgt tggtcccatt tccgaagtta tctcatactt caaaatccca aaataaagct
      241 ccaccccggg gtaagctttc cctttgacaa taaccttgcc atctaaattc ttgtttatct
      301 ccttttccag ctccattctt cgagttttca gtttttccat ttgcggccca atattactct
      361 ccacatcttt cagctttcta actaccatct ggtctttatc tgagagctca ccgtccatct
      421 gcatcttttc aaaagtgtat agtactccct tcactctttg tgtgtcttga cttaattttt
      481 ctaactcgta ttcaatttgt ttgtattcct ccatcagggt acggtcataa gctactctga
      541 tcttggtgga agtcccctct ttatcaccca atatgccagc ctcaatcacc ttgccagccg
      601 cacaatctcc tcccacgatg acccctttgg gaccaacaac tttcaagtta ttattagcta
      661 aaagagttgc gcccgccgcc tctcccccaa ccagaatatc ctcagctgaa atcacaagtt
      721 gattcttgat gtactttact atgactttcc cccggcaatc caaatctcct ttgcctgaac
      781 cagaaaagcc tcccttaatt agaacattcc ctcccaccat taacctggca tcctccacac
      841 tgccatccac ctccaaattc ccatccacgc ttatggagaa acccttctta atactcccca
      901 gcacccgcag ggagccaacg taattcacat ttccgatggt gtagtccaca tttcccttca
      961 agatccgcaa aggttgtata tgaaccctct gatttttgaa aatgatatcc ccttctatcc
     1021 tggaaataag atctaaaggt tggtttggat ccggcatgct attcaatcca accgggagtc
     1081 ttatatcctt tggtgcccga actaacgttt tctttccagt aaccgtggtg ccgaattctc
     1141 ccggcttagc aggaattttt ttgactaata tctggccggc cttgacgagc tgaattatgc
     1201 catgctcttt gaaagccatc ctcccatccc gtccaatttt aaaatcctca cccggcatag
     1261 cgttgaacag aaactccaga atggcatcct cacccaaaac ggactcttcc ccatgtgcaa
     1321 cttcaactct ctgattgtaa accttctgtt ctagtatctc ctgaattgcc gtttcatcta
     1381 agccaaaaca tatcccctgt tctttgagat agctctcgac ttcttctgtg gtcggtttag
     1441 tataaccctc ggcagatggc tgaatcagta aatacgcctt catctcattt ggggagagaa
     1501 aaagttcaaa aacgacaccg ttctttttat cctttgcttt tgatatcgaa gtagcaaagg
     1561 cagatgaatt attagttagt tccatattct cgatttagac tacgagatta tccagggaca
     1621 gagtcctcaa tttattcacc gtttccagca ccctgtcgct tctctcgagg attttcttta
     1681 agttgacttc gaggttacca accagctccc tgtttttgtt caacttcacc tctccatcct
     1741 cggtgagata atacacttcc cccatctcca tacgcaccat tctaatcatc tctaagattt
     1801 gattggcggc ggagttgctc ttatccacca ggtccattat ctcttcacta actttgtatg
     1861 gaatgcagtt cgcctctctg gcgcgagctg aagccccagc caaatctaaa gacactaact
     1921 tagcttcttc agcaaacctc tccaccgaag ccagtatatc ttccaccctg gcggtcttac
     1981 ctttgatagt cataggttgg gttacctttc aaccggtacc tttacctcta aaagctcacg
     2041 caccttcaat cttaactcat ctacattagc tgatttgacc aaataggcgt ccgccagcca
     2101 ggattgaaaa tcgctcttgt aagtggagta agcggaattg agaatgatgg gcatatttct
     2161 cttgcttttt cttagttcag ccagagtctc caatccactt attccctcca ttttgatatc
     2221 taaaatcacc aggtcgatgt caccttcttt ggccatctga atggcttgtt cgccactctg
     2281 agccaacttc acctgaaaac catcatcgct gaataccttt tgatacaact tgaggatatg
     2341 ctcctcatcg tccactaaca gaaccgtctt catctactcc tccttcttca acgggaattc
     2401 tatgtaaaag gtcgtgcctt taccttcctc gctctccact ccaatgaagc cattatgatt
     2461 ttttatgatc tcgctagcca ccgtcaagcc caaaccatta gaatccggct tggtggtata
     2521 gaaagccttg aatacgttat ccagtctctc tcggggaatg ccgcaaccgg tatcagctac
     2581 gctcatcttt acactagcat tggtcaaaga ggtaccgatc gagagtgttc ctccctgagg
     2641 cattgccgca atagcattcc tgaagatatt gaccaaaccg tgtcttatct ggtctgggtt
     2701 aaccaggatc aggggtaatg gactacagta attcctcttg gcccatatct tgccatcttc
     2761 gatttccttc cccagcatat ccagggtctg ctcgattatc tcatttaaat cagtgttttc
     2821 ccaatgtgcc ctttctggtc gagtgaaatt cagcacatta tcgagtatat tctccatcct
     2881 ttgactttcc tgggagatta ttttgaggta ttcgtaattg ggatcgtttg gatccaattt
     2941 ctttagaatc gaattggcga aaccgccaat gatggcgagg ggattgcgca actcatgagc
     3001 tacttgagag gttatctctc ccatcaccga gagcttttcc acccgcacta acctctcgct
     3061 gccttcctta agacgttgat tcgccttttc cagctcagct acctgatgag ccaatttttc
     3121 atacagctta gcactttcaa ttgcggcgct ggcatgataa gccaaaacct ccaaaaattc
     3181 gactttctct ctttcaatcg acttgccgct gatcaggtta tccgccaaga ttatcccaat
     3241 cgacttgccc ttggacgcta agggaactgc tacaaaactc gaagtaccca gaagctcgca
     3301 caaggatttg ccggagccac gctcctctat agaacagttc acgatttcgc aactttgacc
     3361 atccattgcc cgaacaagta gattttcttt atcctgtata ggtatcctta tcttcttaac
     3421 caagtggttg acccaagcat cccgttcctc gaaatgttca tcatggctct ccagaagctt
     3481 tctaaaagat ggcttggttt gagaaagata tgaccagatt ctccctgcct cttcttgatt
     3541 ggctggcccc attgccatct tcccttctaa gtaggtctcg tcttgattca aaagcaaaag
     3601 aaaaacgcga ttgaaagcca ggccttctct ggcagtgaag gctatcatca ctatcctcaa
     3661 tacatcttcc aacttgtggg tgctctttaa gctcttccct aactcgctca ttatctcaag
     3721 ccgcttgatc ccattctcca actcctcttt caatctaatt ccctcagtga tatcttccac
     3781 atagctcaaa ctccccattg tcttaccgtc agacaacacg ggaaatgatt tcagccgcaa
     3841 atacgcctcg cctccgtcct gcctcatata cttcaaccct tccagcacaa actccttccc
     3901 caaagctagg cactccctca tttgggaaga gactttcgct agacttggaa ggtcaaagat
     3961 attcctgcct aaaagctcat tggggtcgga gtaactctgg tctttaggca aatgtggatt
     4021 agcatgagtt atcacaccgg acgcgtcaca taccagaaca cccaatggaa tcttgtccag
     4081 aagcgcattc aaggtatcca ggggaactgt cttctcaagc ttaatcatat tcttcactta
     4141 gttgattcgt ctaaattcaa catcttatct ttatggccat caatgtaatc ttccactacc
     4201 aattttttta tcttaagcag cttgcgcgtt acttgtgaaa tctgattgac ttcgttatca
     4261 attgctttta acctcttctt cacattgggg ctgacgtttt tcatagtcat caagagatat
     4321 tgtgtattgc cagcgatcac tgccagagga ttattgatct catgattgat gctggtggct
     4381 acctgcgaga tggcggagag cttctcatta tcaactgagc gctccctcgt ctccttcaaa
     4441 agattttgtg cttgctccaa ctccttgttc ttcttctcca actctgtttg atataacatc
     4501 ctttcgatga agatggaaac ttgcccagta aagatggtta agaaatccaa gtcgctcttg
     4561 gtgtaggcat catcccggat gtggctcaaa ttcagcaccc ccaccaattc gttctttatc
     4621 agcaagggaa tcgacacaaa ggaatggatg ctgtttttcg agttatggct tttgcggtga
     4681 agatgggata taaatatcgg ccttttctct ttggc
//
LOCUS       DASWLN010000040         9201 bp    DNA     linear   ENV 20-FEB-2024
DEFINITION  MAG TPA_asm: Candidatus Zixiibacteriota bacterium isolate
            SMAG_U2949 k141_779937, whole genome shotgun sequence.
ACCESSION   DASWLN010000040 DASWLN010000000
VERSION     DASWLN010000040.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37960594
            Sequence Read Archive: SRR10376091
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Candidatus Zixiibacteriota bacterium (soil metagenome)
  ORGANISM  Candidatus Zixiibacteriota bacterium
            Bacteria.
REFERENCE   1  (bases 1 to 9201)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 9201)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (26-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/12/2024 08:28:26
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 1,440
            CDSs (total)                      :: 1,416
            Genes (coding)                    :: 1,412
            CDSs (with protein)               :: 1,412
            Genes (RNA)                       :: 24
            rRNAs                             :: 1 (16S)
            partial rRNAs                     :: 1 (16S)
            tRNAs                             :: 21
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 4
            CDSs (without protein)            :: 4
            Pseudo Genes (ambiguous residues) :: 0 of 4
            Pseudo Genes (frameshifted)       :: 0 of 4
            Pseudo Genes (incomplete)         :: 3 of 4
            Pseudo Genes (internal stop)      :: 1 of 4
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..9201
                     /organism="Candidatus Zixiibacteriota bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_779937"
                     /isolate="SMAG_U2949"
                     /isolation_source="soil"
                     /db_xref="taxon:2053527"
                     /environmental_sample
                     /country="USA: Oregon"
                     /lat_lon="42.99 N 123.63 W"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            complement(<1..1562)
                     /locus_tag="VGB16_01595"
     CDS             complement(<1..1562)
                     /locus_tag="VGB16_01595"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEX9916406.1"
                     /translation="MKNFERFSIWGLFLFLFLSFQTSYALTNYMFFSVNGDTSLDSMV
                     QGDTLSWGANCAIGAQTHWQIWIDLDQDSLLDNPGDKLLFEFVIADGDTLSEGPPPDI
                     SPTPDGWFICQPFEAGIAPAHYIFKVVDLTDSTTAQRMIWVTPLPSPPNKFTGWITIP
                     GHTAPDTLLKNIWVEAEDSISGSAFWAGLSNDSGYYEINVGSNGTGHIFYIISSDVAG
                     YVTPAEKTEIVSGVVDSVNFSYELPADSIFGEVRDQDDSLLVLNLSMWCSPRFSGPSY
                     KNYESTDGKYKILFGSSELGRWDLGLDSDNLVPDYLVPNGFEFNNQTQHSIEHNFTCQ
                     KTDTAIYGRVTESGGLPTHSYRIRAESSTLQSWTDVVSGTGSNNNFTLHVSSLDSSSW
                     YVNIYTSDDRYPIPAGYALEGGGELNVSPGDTVLLNFISGKMARDTIKVDTPDPGINW
                     DHVSLSFYASGKNYGGNPGSNGIYAIYVDTGSYNLTVNCSGYLSNPGSRNVVISSDTS
                     GGLGFILNYTHAR"
     gene            complement(1916..2686)
                     /gene="lpxA"
                     /locus_tag="VGB16_01600"
     CDS             complement(1916..2686)
                     /gene="lpxA"
                     /locus_tag="VGB16_01600"
                     /EC_number="2.3.1.129"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_012546244.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008780 -
                     acyl-[acyl-carrier-protein]-UDP-N-acetylglucosamine
                     O-acyltransferase activity [Evidence IEA];
                     GO_process: GO:0008610 - lipid biosynthetic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="acyl-ACP--UDP-N-acetylglucosamine
                     O-acyltransferase"
                     /protein_id="HEX9916407.1"
                     /translation="MNIHPTAIIDKKAEIERDVSIGPHTIIQGKVIIGQGTKIASNIL
                     LDDGTIIGKNCSIHHGAVLGTPPQDLKFSEEKTILKIGDNCIIREYVSINRGTKHRGQ
                     TTVGNNCFFMMYTHVAHDCIIGNNVIMANSVNLGGHVEIEDYAIVGGIVPVHQFVRIG
                     THSIIGGGFRAVQDVCPYALAAGYRLRIVGLNLIGLKRRGFPQETIHILGKAFRMLFH
                     SKFNTTQAIEKIKSELEPIPEINHILDFIAKSERGIVK"
     gene            complement(2683..4083)
                     /locus_tag="VGB16_01605"
     CDS             complement(2683..4083)
                     /locus_tag="VGB16_01605"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_006365511.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008759 - UDP-3-O-[3-hydroxymyristoyl]
                     N-acetylglucosamine deacetylase activity [Evidence IEA];
                     GO_function: GO:0016836 - hydro-lyase activity [Evidence
                     IEA];
                     GO_process: GO:0006633 - fatty acid biosynthetic process
                     [Evidence IEA];
                     GO_process: GO:0009245 - lipid A biosynthetic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="bifunctional UDP-3-O-[3-hydroxymyristoyl]
                     N-acetylglucosamine deacetylase/3-hydroxyacyl-ACP
                     dehydratase"
                     /protein_id="HEX9916408.1"
                     /translation="MDKLQRTIKKPASYSGTGLHTGNQTTVTFKPAGIGQGIVFVRGD
                     LESQPRIPAVIENVVDITRGTSIGKGNIKICTVEHVLAALAGLELDNIIVELDSNEPP
                     VGDGSCLPFVEVLLKAGIVEQEQVKSYIEIDTPLFYSEKERGVDIVVLPSDELRMTFM
                     IDYKNPALGTQYTSLVSLEEEFVKEFAPARTFCFLSEVEMLREQEIIKGGALDNAVVI
                     VDRQMDKKELSRLKKLFDLKENMFVGKSGILNDVKLRFPNEPCRHKAIDLLGDLYLLG
                     APLRAHVLAARSGHAANAELIKKIKREHEKKKIAQKYQKERKGDFLLDINAILKIMPH
                     RYPFLLIDRIVDLEPSKKVVALKNVSINEPFFSGHFPGNPIMPGVLILEGMAQAGGFL
                     LLHTLENPQKSVVYFMGIDDVRFRKPVIPGDQLRFEMEMLFFRRGTCRIQGKAFVEHD
                     LVAEATLMATITDQRK"
     gene            complement(4211..4498)
                     /locus_tag="VGB16_01610"
     CDS             complement(4211..4498)
                     /locus_tag="VGB16_01610"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEX9916409.1"
                     /translation="MITFFKTYFSWLDQLSQMAYKHFGLLGQMVFFILIFYLVYLIVT
                     RIIKSIFDLALWVVLPSVLLSATAAYLLSFNFFQILPVCACLMIIISFVRH"
     gene            complement(4537..4767)
                     /locus_tag="VGB16_01615"
     CDS             complement(4537..4767)
                     /locus_tag="VGB16_01615"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEX9916410.1"
                     /translation="MNLRVKFTLTMENLIVENNYIDNITISWISDVSQQEVLDMSQEW
                     ITTKDFLTQKMTGLQKVGESSLTIEPVEESAV"
     gene            5008..6387
                     /locus_tag="VGB16_01620"
     CDS             5008..6387
                     /locus_tag="VGB16_01620"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_012874550.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0005886 - plasma membrane [Evidence IEA];
                     GO_function: GO:0015205 - nucleobase transmembrane
                     transporter activity [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="NCS2 family permease"
                     /protein_id="HEX9916411.1"
                     /translation="MLDRTFKLKELRTDVKTEFLAGLTTFLTMSYIIFVNPLFLSFFG
                     ESQFKDLALPFSASMTATCLAAAVMCILMGIWTNYPFALAPGMGLNAIVAYQLVLGMG
                     LSWPSAMGVIVIEGLVITLLVITGFREAVMDSIPLSLKQAISVGIGLFLAFIGLKEAG
                     FMKASESTLVTLGDLTKLPVIVSFLGLVLTAVFIKRKVKGALLIGIVASTLIAVIINY
                     ISGFKAYPASEYARIPQHILSIPDFSTIGHFDFGVFAKVGLISAVVVIFSIMLSDFFD
                     TLGTVIGIGGEGKFLTPDGKLPRIKQVLLVDSLAAAMGGVTSASSVTTYIESAAGISV
                     GGRSGLTNIFVGILFILALFFSPLAGIIPKQAVAPALIIVGFLMLPIIKEIPFAKFDE
                     AFPAFLIMILMPLTYSISNGIGAGFILFALIKLLTGKGKEVHWLMYIVSLAFAVDFAI
                     PVLKMVFGF"
     gene            6472..7035
                     /locus_tag="VGB16_01625"
     CDS             6472..7035
                     /locus_tag="VGB16_01625"
                     /inference="COORDINATES: protein motif:HMM:NF013921.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003677 - DNA binding [Evidence IEA];
                     GO_function: GO:0004803 - transposase activity [Evidence
                     IEA];
                     GO_process: GO:0006313 - DNA transposition [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="transposase"
                     /protein_id="HEX9916412.1"
                     /translation="MGKQRVYTLPNQVHFITTNVFQRIPIFSNVDYCKIVIQKINFYR
                     KRYTFKLIGYMIMPDHIHLLIQPPEKQNISSIIRDLKRSIAMEIINRLKRENKAGLLK
                     SLKLPNVRSKNHTFSLWQRRFIDFNIFSEKKLYEKLEYIHNNPVRGKLVSSPSDWIFS
                     SYRNYFEEDDSMIAIDKIAMTYSPMKK"
     gene            7176..>9201
                     /locus_tag="VGB16_01630"
     CDS             7176..>9201
                     /locus_tag="VGB16_01630"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEX9916413.1"
                     /translation="MKVPLRYIMWGVLFLFFFGQSSWAKSIGSASKSQKVINHEKLEA
                     AEEYLNSKTLSGEFGPHGSYGGPNSYNYHWVDSDTNLGITYGWIEISGGGTLINTSEW
                     HNSIAPAIRLDDGTAGPYYLGFSFPYCDSTYDSIYVGTNGVLSFSDSELTDSGYFNYD
                     YYCNGITIPYMRFPNAISPLYVDMNLDPATNHGGGYVYYWKNAAGDTFIVEYKDVRPY
                     AYDYSPNDSATLEVIFATIDTSITFQYKHIEFHDSLGYNPQGFDFNERSLVGIQDGSR
                     KIGLRYHSGFQNWWRYEGMNNSPHNGLAVKFKRDEIIQHNLTSVYYKQGASTYEPYNW
                     VYYYSWDLDKTYDIESSWFFNTGKYDEIGVPVSCDIYAMDTAGNAVSFVQGFNTTLSD
                     IGAHDSAQAVFSGNWQPPARGRYKVIYKANLPGDELSFDDSLNRNAYAERKTMSSNWA
                     NIVPVCDGHIEALEWGDAFKQDISYFKEGDGMLSTPPYSDSNAVFLYVKNDSNFIYLA
                     VDAKADTTDTRTDRVKIVIDDNTNGFFEADSSEGFLMFWNYPSLEDSLFFTEEVSQNN
                     PPYYYGMPRDTYTVSGVDYGFSKNGSNMQVEMAIPFGDEHWKINKTSLDSVGICVYYM
                     DWGDSIYFQGSPSGLWMPHVIGSWPWYSMSHYYPGYLGRIVLNSTTDVGDE"
BASE COUNT         2570 a         1914 c         1945 g         2772 t
ORIGIN      
        1 cgagcatggg tataattcaa gataaaccct aaccctccgc tggtatcgga gctaataacg
       61 acatttcgag aaccaggatt ggatagataa cctgagcagt taactgtcag attatagctt
      121 ccggtgtcaa cgtagatggc ataaatacca ttgctcccgg gatttccccc ataattttta
      181 ccggatgcat aaaagctcaa cgagacatga tcccagttta ttccaggatc cggcgtatca
      241 acttttatag tatcccttgc cattttcccg cttatgaaat taagcaacac agtatctccg
      301 ggtgagacgt tcaattcccc tcctccttct aaggcatatc ccgctggaat cggatatcta
      361 tcatccgagg tatatatgtt cacataccag ctggatgaat ctaaggagga gacgtgcaat
      421 gtgaaattat tatttgagcc agttccggag actacatcag tccagctctg tagcgtactg
      481 gactcggctc gaattctata agaatgagtt ggcaatccgc ctgattcggt cactcttccg
      541 tagattgccg tatccgtctt ctggcaggtg aagttgtgtt cgatgctgtg ctgggtttga
      601 ttgttgaatt caaaaccatt gggaaccaga taatcgggaa caagattgtc tgagtctagt
      661 cctaagtccc acctccctaa ctccgaggag ccaaaaagga tcttatattt cccgtctgtg
      721 ctttcgtagt tcttatagct tggtccgctg aatctcgggc tgcaccacat cgacagattc
      781 aaaactaaca aagaatcatc ctggtccctc acctctccga agatactatc tgccgggagt
      841 tcatagctga aattaacgct gtcaaccacg ccggagacga tttcagtttt ctcagctgga
      901 gtaacataac cagctacatc agaagatatg atgtagaaaa tgtgtccggt gccattgctt
      961 cccacattga tttcataata accgctatca tttgaaaggc ccgcccagaa ggcagatcct
     1021 gagatgctat cctctgcttc tacccagata tttttcaaaa gcgtatccgg agcagtatga
     1081 ccgggtatgg ttatccagcc agtgaacttg ttaggtggtg aaggaagtgg ggtaacccat
     1141 atcatacgct gggcagtggt gctgtctgtc agatctacaa ctttgaagat ataatgagcc
     1201 ggtgcgattc cggcctcaaa gggctgacag ataaaccagc catccggggt gggactgata
     1261 tccggtggcg gaccctcact caaggtatca ccatctgcta tgacgaactc aaaaagcaat
     1321 ttatccccag gattatccag taatgaatcc tgatcaaggt ctatccagat ttgccagtgg
     1381 gtttgcgccc cgatggcaca gtttgcgccc cagcttaagg tatcaccctg aaccattgaa
     1441 tctaaactag tatccccatt aaccgagaag aacatatagt tggtaagagc atagcttgtc
     1501 tggaaactta aaaataaaaa cagaaaaagg ccccaaatac taaacctttc aaaatttttc
     1561 atatacaccc ctttcttcaa atggtttctt ggttttttta aaatccaaaa ttctaacaat
     1621 ctcttttatc acccccttgt tagtagtcct attgtaaaaa tttgttactg aatatctatt
     1681 tttgcaatag tggggttgaa agtaaaaacc tttccctaat tatttttatc ggcagattgg
     1741 tttaacaata taagatattt tgtaaaaaga tgaaaattag agggcaattt atgaattatc
     1801 tttgttttca tctttttcac gtctttcgat ttttaaaata aagaccacca agaaagcagt
     1861 ggtcatcttt tgaggaaata taatagaaag aaaggagaca gtcaaatgaa gatggttatt
     1921 ttacaattcc cctctcgctt ttggcgatga agtctagaat atggttaatt tctggaatgg
     1981 gctcaagttc agatttgatt ttttctatgg cttgtgtggt gttaaacttg gaatggaaaa
     2041 gcatcctgaa cgctttgcct aagatatgaa tggtctcctg gggaaacccc ctccttttta
     2101 gtccgattaa gttcaagcca acgattctta aacgataacc agcagccagg gcgtaagggc
     2161 aaacgtcctg taccgccctg aaccctccac caattattga atgtgtgcct atgcggacga
     2221 attgatgcac tggcacgatc ccacccacaa tagcataatc ctcaatctcc acatgccctc
     2281 ccagattgac cgagttagcc attatcacgt tgttcccgat tatacagtcg tgagcaacat
     2341 gggtgtacat catgaaaaag cagttgttcc ccaccgtagt ttggccccga tgtttagttc
     2401 ctctgtttat gctgacatat tcgcgaatga tacaattgtc cccaattttc aaaatggttt
     2461 tttcttcaga gaatttcaaa tcctgaggcg gtgttcccaa aaccgcacca tggtgaatgg
     2521 agcaattctt gccgattatg gttccgtcat caagtaagat attggaggca atcttagttc
     2581 cttgcccgat tatgaccttc ccctggataa tagtatgggg accaatggag acatctcttt
     2641 ctatttctgc tttcttgtca attatggctg tagggtggat attcattttc gctggtcagt
     2701 gatcgtagcc atgagcgtag cttcggcaac caagtcgtgt tccacgaaag ccttaccctg
     2761 tatgcggcag gtccctctac ggaagaacag catttccatt tcaaaacgga gctggtcccc
     2821 gggaatgact ggtttcctaa agcgcacgtc atctattccc ataaagtaaa ctacactctt
     2881 ctgtgggttt tccaatgtgt gcaggagcaa gaatcctcct gcttgagcca taccctcaag
     2941 gattagcact ccgggcataa taggattgcc agggaagtgc cctgaaaaga atggttcatt
     3001 tatgctcacg ttttttaggg ctactacctt cttgctcggt tccaagtcca ctattcgatc
     3061 tattaagagg aagggatagc gatggggcat tatctttaga atagcattta tgtctaacag
     3121 aaaatcgccc ttacgttctt tttgatattt ctgggcgatc tttttcttct catgttcccg
     3181 ttttattttt ttgatgagtt ccgcattggc ggcatgacct gagcgtgcag ccagaacatg
     3241 ggccctaagt ggtgcaccca aaagatacaa atcacccaaa aggtcgatgg ctttatgtcg
     3301 gcagggctca ttcggaaatc gaagtttgac gtcgtttaaa atcccgctct ttcccacaaa
     3361 catgttttcc ttcaaatcaa aaagtttttt caatctactt agctctttct tatccatctg
     3421 gcggtcaact atcaccactg cgttgtccaa agcccctcct ttgatgattt cctgttccct
     3481 caacatctcc acctcactta ggaaacaaaa agtcctggca ggggcgaact ccttaacaaa
     3541 ctcttcttcc aaagatacca gagaagtgta ttgagtgccc agggcggggt ttttataatc
     3601 tatcataaaa gtcatcctca gctcatccga aggcaggacc acaatatcaa cccctctttc
     3661 tttttcagaa tagaaaagag gggtgtctat ttctatatag ctcttgactt gctcctgttc
     3721 aactattccg gccttaagta agacctccac aaacggcaaa caacttccat ccccaactgg
     3781 aggttcattt gagtccaact ccacgataat gttatccagc tccagaccag ccaaagcagc
     3841 caaaacatgt tcaactgtac agatcttaat gttacccttg ccgatagagg tgccacgagt
     3901 tatatccacc acgttttcga ttacagctgg tatccgaggt tggctttcta aatcccctct
     3961 cacaaagacg attccttgac caatcccggc cggcttaaag gtaaccgtgg tttgatttcc
     4021 agtatgtaat cctgttccgg aatatgatgc tggcttcttt atagtgcgct gcaacttatc
     4081 catacttccc ctgaatcaaa atatttggtt aactaataga ataaagaatt ttctagctaa
     4141 aagtcaagca caaaatcctt gatttcgtct tgacaatatt aagaggatag gcttatcttt
     4201 ttggaaagga ttagtgcctt acaaagctta taatgatcat taagcacgca caaacaggta
     4261 agatctggaa gaagttgaag ctcaagagat aggcagcagt ggcggacaac aaaacagaag
     4321 gaagaactac ccacagagcc aagtcaaata tgcttttgat tatccgggtg acgatcaaat
     4381 agacaagata aaagataagg atgaaaaaga ccatctgacc caataggcca aaatgtttgt
     4441 aagccatttg gctaagttgg tctaaccagg agaagtaagt tttaaaaaaa gtaatcatga
     4501 ccaaaaggat atagtttatt tgggaaagca tgatatctag accgcagatt cttccaccgg
     4561 ctcaatagtc aggctcgatt ccccaacttt ctgtaagccg gtcatctttt gggtcaagaa
     4621 atctttggtg gttatccact cctgggacat atccaatacc tcctgctgag agacatcgct
     4681 gatccaggag atagtgatgt tatctatgta gttattctct actataaggt tttccattgt
     4741 taaggtgaac ttcactctca agttcatcac atccttgttg gtttaaggtt atgtaatctc
     4801 tttgcaatct gaaaaagcaa cccttatgcc agaaatcagg cggcatcaaa ttacaaaatg
     4861 caatacgata actcatttaa caacaaagag gtaaaagggg aaataattgc ttggtcaata
     4921 ttttagcaag aaattggatt ttattctaca aaaagaatag gaatcaatct acaatatgtg
     4981 gcttatcctc taacctaagg agggtctatg ctggatagga ccttcaagct aaaggaactg
     5041 agaacagatg ttaaaaccga gtttttggca ggtttgacca cttttctaac tatgtcctac
     5101 atcatcttcg tgaatcctct ttttttgagc ttctttggtg aaagccaatt taaagatttg
     5161 gccctgccat tctccgcctc tatgactgca acctgcttgg cagctgcagt aatgtgcata
     5221 cttatgggca tctggaccaa ctatcccttt gccttggccc cgggaatggg cttaaatgcg
     5281 attgtggctt atcaattggt tctgggaatg ggtctttcct ggccatcagc tatgggggtg
     5341 attgtgattg agggtctggt tatcacttta ttggttatca ctgggtttcg tgaggcggta
     5401 atggactcaa ttcccctatc tttaaaacag gccatatcag ttgggattgg actgttcttg
     5461 gcattcattg gactgaaaga ggcaggattt atgaaggcaa gcgaatcgac tctggttact
     5521 ttgggcgacc tcactaagct tcccgttatc gtttcatttc tgggccttgt cctcactgcg
     5581 gtttttatta aaagaaaagt taagggagct cttttgatag gaatcgttgc ttcaactctg
     5641 atagcggtga taataaacta catttccgga tttaaagcct atccagcttc agaatatgcc
     5701 cgcattccgc agcatatctt gtcaattcct gatttttcta ctatcgggca ttttgatttt
     5761 ggggttttcg ccaaagtggg tctaatctcc gcggtggtag tcatattttc cattatgctt
     5821 tcagatttct ttgatacttt gggaactgtt attggaattg gcggagaggg gaagttttta
     5881 accccggacg gaaaactccc cagaatcaag caagtccttt tagttgattc cctggctgcg
     5941 gctatgggtg gggtaacaag tgccagctca gttacaactt acattgaaag cgcggctggc
     6001 atatcagttg ggggaagaag tgggctaacc aacatatttg tcgggatttt attcatcttg
     6061 gcactctttt tctcacctct ggctggaata ataccgaaac aggcagttgc accggctttg
     6121 attattgttg gattcctgat gcttcctatt ataaaggaga ttccctttgc caagtttgat
     6181 gaggcgttcc cggctttttt gattatgatt ctgatgcctc taacctacag catctccaat
     6241 ggaattggtg ccggttttat ccttttcgct ttgattaaac ttctaactgg aaagggaaaa
     6301 gaggttcatt ggttgatgta tattgtctct ttggcttttg cagtagattt tgcgattccg
     6361 gtgttgaaga tggtatttgg attttaacgc aacggcgcag ccgttactta cccttatcat
     6421 aacctatata ttgggtaggg aacggctgct gccgttccct aaagagtata aatgggaaaa
     6481 caacgagttt atactttacc taaccaagtt cattttataa cgaccaatgt ttttcaaaga
     6541 attcccattt tctcaaatgt cgactattgt aaaatagtta tacaaaagat aaatttttac
     6601 aggaaaagat acactttcaa gctgattggt tatatgataa tgccggatca tatacatctt
     6661 ttgattcaac ccccagagaa acagaatatt tctagtataa ttcgagattt gaagcgttcc
     6721 atagctatgg agatcattaa tcgtcttaag agagaaaata aagctgggtt gttaaagtcc
     6781 cttaaactgc ctaatgtcag aagcaaaaat cataccttta gcctatggca gagaagattt
     6841 atagatttca atatcttttc cgagaagaaa ctatatgaga agttggaata tatacataac
     6901 aatcccgtaa ggggcaaatt agttagcagt ccatctgatt ggatattctc cagctacaga
     6961 aactactttg aagaggatga ttcgatgatt gcgatagata agattgcgat gacctattcg
     7021 cctatgaaaa agtaatggcg cagccattac ttaccccaat tcacgattct ggtgttgaag
     7081 aagcgttcag attttgaata aagtgtgttg acaaattctg ttttatgatt attattagct
     7141 tactttccaa tcacaattca atcaaaggag ggtgtgtgaa ggtaccgcta aggtacatta
     7201 tgtggggggt tttatttttg ttctttttcg ggcagagcag ttgggctaaa tcaattggct
     7261 ctgcatctaa aagccagaag gtgataaacc acgagaaact ggaagcagca gaagaatatc
     7321 ttaacagcaa gaccttaagt ggagagtttg gtccgcacgg aagctatgga ggtccaaata
     7381 gctacaatta ccattgggtg gatagcgata ctaacttagg gattacttac ggctggattg
     7441 aaatctccgg tggggggact ctgataaata cgagcgagtg gcataattct attgcgccgg
     7501 caattcgctt agatgatgga accgccggac cttattattt ggggttctct tttccctatt
     7561 gtgattcgac ttatgattca atctatgtcg gaaccaatgg agtcttatca tttagtgata
     7621 gcgaactgac tgatagtggc tactttaatt atgactatta ttgtaatgga ataactattc
     7681 cttatatgag gtttcctaat gctatctctc ctttgtatgt tgatatgaac ttggaccctg
     7741 ctaccaacca tggcggagga tatgtatatt attggaaaaa tgcggctgga gatactttta
     7801 ttgttgaata caaagatgtt agaccttacg catatgatta ttctccaaat gactcagcca
     7861 cccttgaagt tatctttgcc acaatcgata cctccatcac ttttcaatac aaacatattg
     7921 aatttcacga ctccttagga tataacccgc aaggattcga cttcaatgaa cggtccttgg
     7981 tggggattca agatgggtcc agaaaaattg gacttagata tcatagtggc tttcaaaatt
     8041 ggtggcgtta tgagggaatg aataattctc cacataacgg cttggcagtt aaattcaaaa
     8101 gagatgaaat cattcaacac aatctgactt ctgtttatta taaacaaggt gcttccactt
     8161 atgagcctta taattgggtt tattactatt cttgggatct ggataaaacc tatgatattg
     8221 aatcttcttg gttttttaat actggcaaat atgatgaaat aggtgtgcca gtctcttgcg
     8281 atatttatgc aatggataca gctgggaatg ctgtcagttt tgtgcaggga ttcaacacaa
     8341 ccctctctga tattggtgcg cacgattcag cccaagctgt cttttctggg aattggcaac
     8401 cgccagcaag agggagatat aaggtgattt ataaggcgaa cttgcccgga gatgagctct
     8461 ccttcgatga ctccctgaac cgcaacgctt acgctgagag gaaaacaatg tcatcaaatt
     8521 gggctaatat cgttccggtg tgtgatggac atattgaagc tctcgagtgg ggtgatgcgt
     8581 ttaagcagga tatttcatac tttaaagaag gagacggtat gctttcaacg cctccttact
     8641 ccgactcaaa cgcagttttc ctttatgtga aaaatgactc caacttcatc tacttagcag
     8701 tagatgccaa ggctgataca acggatactc gtacagatcg ggtgaaaatc gtaattgatg
     8761 acaataccaa cgggtttttt gaggcagatt cttcagaggg gttcttgatg ttctggaatt
     8821 atccttccct cgaagatagt ctcttcttta ctgaagaagt ttcacagaat aatccgcctt
     8881 actactatgg tatgccccgc gatacctata ctgtgagtgg agttgattac ggatttagca
     8941 aaaatggttc taatatgcag gttgaaatgg cgattccatt tggagacgaa cattggaaaa
     9001 taaacaagac ttccttggat tcagtaggaa tctgcgtcta ttatatggat tggggggata
     9061 gcatatattt ccagggttcc cccagcggac tttggatgcc acatgtgata ggttcttggc
     9121 cctggtatag tatgtcacac tattatcctg gctatttggg taggatagtg cttaattcta
     9181 ctacggatgt gggagatgag g
//
LOCUS       DASWLN010000041        14454 bp    DNA     linear   ENV 20-FEB-2024
DEFINITION  MAG TPA_asm: Candidatus Zixiibacteriota bacterium isolate
            SMAG_U2949 k141_423058, whole genome shotgun sequence.
ACCESSION   DASWLN010000041 DASWLN010000000
VERSION     DASWLN010000041.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37960594
            Sequence Read Archive: SRR10376091
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Candidatus Zixiibacteriota bacterium (soil metagenome)
  ORGANISM  Candidatus Zixiibacteriota bacterium
            Bacteria.
REFERENCE   1  (bases 1 to 14454)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 14454)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (26-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/12/2024 08:28:26
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 1,440
            CDSs (total)                      :: 1,416
            Genes (coding)                    :: 1,412
            CDSs (with protein)               :: 1,412
            Genes (RNA)                       :: 24
            rRNAs                             :: 1 (16S)
            partial rRNAs                     :: 1 (16S)
            tRNAs                             :: 21
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 4
            CDSs (without protein)            :: 4
            Pseudo Genes (ambiguous residues) :: 0 of 4
            Pseudo Genes (frameshifted)       :: 0 of 4
            Pseudo Genes (incomplete)         :: 3 of 4
            Pseudo Genes (internal stop)      :: 1 of 4
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..14454
                     /organism="Candidatus Zixiibacteriota bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_423058"
                     /isolate="SMAG_U2949"
                     /isolation_source="soil"
                     /db_xref="taxon:2053527"
                     /environmental_sample
                     /country="USA: Oregon"
                     /lat_lon="42.99 N 123.63 W"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            complement(<1..589)
                     /locus_tag="VGB16_01635"
     CDS             complement(<1..589)
                     /locus_tag="VGB16_01635"
                     /EC_number="2.3.1.-"
                     /inference="COORDINATES: protein motif:HMM:NF024917.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="GNAT family N-acetyltransferase"
                     /protein_id="HEX9916414.1"
                     /translation="MEIITYPELKNKDEFMMLMEMAFWWPVVPRHFDRITQWDERLKG
                     SPIGFCAMENGRLASHVGVMDIPLRTLKGEVEVVGGISAVATNPDFVNRSLAGILMEK
                     AHRYFREKGYRFSFLCTNRTIRAHAGYKKLGYVDVECINHIPSVYKILKKNSSAKKPT
                     KRELNPNKVYSLFEKFVKDKTGFVIRQKDFVKLYLF"
     gene            complement(693..1610)
                     /locus_tag="VGB16_01640"
     CDS             complement(693..1610)
                     /locus_tag="VGB16_01640"
                     /EC_number="2.3.1.-"
                     /inference="COORDINATES: protein motif:HMM:NF024917.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="GNAT family N-acetyltransferase"
                     /protein_id="HEX9916415.1"
                     /translation="MEIITYPELKNKDEYMMLMRMAFGWPISLGDIDRIIKLDHWFKD
                     SPIGFCAMENEKLTGHVGVLNIPLKTLEGKTETVGGIRAVATNPEFANKGIAGLLMEK
                     AHAYFKQKGYRFSFLSTRRTLIAYAGYKRLGYVEVESMNRIPTVYKIVQSKHGAKKSI
                     KKKLDPNKIYSLFREFVKDKVGFVIRQKDFVKLCSFLKLIDKKKFVQVKKGYIFLQEK
                     SDAMQVQEIVALDRSTYEKLVIKAEQMSKWAVIDHYVYDPVLLEVYKSRGYKFQLRSN
                     HVLMVKSLDGLDYREVFGDSLYMSVLDWF"
     gene            complement(1942..2460)
                     /locus_tag="VGB16_01645"
     CDS             complement(1942..2460)
                     /locus_tag="VGB16_01645"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_013646028.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016730 - oxidoreductase activity, acting
                     on iron-sulfur proteins as donors [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="ferredoxin-thioredoxin reductase catalytic
                     domain-containing protein"
                     /protein_id="HEX9916416.1"
                     /translation="MNEEVDINQQQIDKLYDRLKKEVESGGYHLNPDVEFTKELVKGL
                     LINEKRYGYWACPCRLASGIKGEDLDIICPCDYRDPDLNDYGTCYCALYVSKDAFDGK
                     KKVSSIPERRPTKDKREKLKEKNSESDLSKTSLSYPVWRCKVCGYLCARNEPPEICPI
                     CKAKKERFEKFM"
     gene            complement(2457..2750)
                     /locus_tag="VGB16_01650"
     CDS             complement(2457..2750)
                     /locus_tag="VGB16_01650"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_010877185.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0015036 - disulfide oxidoreductase
                     activity [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="glutaredoxin family protein"
                     /protein_id="HEX9916417.1"
                     /translation="MGYEEHIKHVSGTKKGDILMFAISTCVWCKKTKKFLGELGVDYD
                     YVDVDLLGEDEKEEAQAEMQRWNPSVSFPTVGIDNKDCIVGYRPEELKEKLGL"
     gene            complement(2844..3707)
                     /locus_tag="VGB16_01655"
     CDS             complement(2844..3707)
                     /locus_tag="VGB16_01655"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_020603454.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0016020 - membrane [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="SPFH domain-containing protein"
                     /protein_id="HEX9916418.1"
                     /translation="MLIFLICLVGVIILLSGIRIVRPTHRGLIERLGKYNRFAGLGFN
                     WIIPFGIEKLYQVNITEQMVDAEPQEIITFDNLNAKVDAQVYFKVKNDEDSVKKSLYN
                     VNNYQWQIVNLARTTLRNIIGTLTLKSANSERGKINDELHKTLMTETASWGIDIVRTE
                     LKEIDPPKDVQETMNKVVKAENEKVAAVDFATATETVADGARRAEIKKAEGIKQAKIL
                     AAQGEAEAIRLVNEAAEKYFVGNAQILRKLQAVENSLKDNAKIVIPAGTELVNVIGEM
                     AGVLPLRGKQA"
     gene            complement(3744..4334)
                     /locus_tag="VGB16_01660"
     CDS             complement(3744..4334)
                     /locus_tag="VGB16_01660"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEX9916419.1"
                     /translation="MKRILIFLLLFLLIFTTSALAFDGQRKGFVLGGGLGFTPVNRWS
                     TGGFNENSVGWGDNTLIGYAWNEKNMIVLEGNGTFYNSDILDVNILQFFLGPSWYHYF
                     GPQESRTFFSVIGIGGYYFGLPPFSSYIRISLNGGEMPPAPPDHARGFGYLMGGGYEF
                     AKHYQIGAYLAGGKTSEYGTNYGNMHIMILLNNIDY"
     gene            complement(4347..4835)
                     /locus_tag="VGB16_01665"
     CDS             complement(4347..4835)
                     /locus_tag="VGB16_01665"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_020381499.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003677 - DNA binding [Evidence IEA];
                     GO_function: GO:0004519 - endonuclease activity [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="endonuclease domain-containing protein"
                     /protein_id="HEX9916420.1"
                     /translation="MAKTKTIYKFIYIPYKRKLTKLAQQNRDNPSPAEKKIWYEVLCR
                     KQFEEYKFTRQKPITNFIVDYYCSKLQLVIEIDGDSHVENKEYDEARTSVLNQYGLKV
                     LRYTNRDVMNNIEGVYQDLMWVVKPPLYSPLSGGQKSKSLLLRREKKHTPVKGLQGKT
                     YS"
     gene            complement(5071..5754)
                     /locus_tag="VGB16_01670"
     CDS             complement(5071..5754)
                     /locus_tag="VGB16_01670"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEX9916421.1"
                     /translation="MSLFNKKQQVELEAFCRLFYDNVILNCVVNGLDVNATILDTLKN
                     SIVATDHNFANIDSQKVNDEIIVLQFELFALAWLHQFGDKLAVAQSTFTKNYLHDKKR
                     DDIWDAMESYNQAIARSSTLGRTSKKPFDRAYLARVDTTRMNLFKQFHKEEHDPKCVA
                     RVLNRLFADDVWKKGVTAGLLMFALCDRLSFDPNCQPSKEAHSRWFIEINDFYDKDLK
                     SLSNIKIKN"
     gene            complement(6173..9157)
                     /locus_tag="VGB16_01675"
     CDS             complement(6173..9157)
                     /locus_tag="VGB16_01675"
                     /inference="COORDINATES: protein motif:HMM:TIGR04183.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0031240 - external side of cell outer
                     membrane [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="T9SS type A sorting domain-containing protein"
                     /protein_id="HEX9916422.1"
                     /translation="MKKAILFFVVVIMGLFVFQDGHSKQFTETEAQQLTADYRVHNQG
                     NLALAISNSGWLGTSASGTQKPFLSCEFPKGSGIEYLYMSGFWVSAVVESETLTSTSM
                     DGWEPIYEFSPKKPYPDPDYFIREIDSTADQEFIAVFTDTVTPTENDDPYDGRPHKPI
                     GLEITLHTYSWVTPPYDDFVILDYKIKNISGKHISGAYLGMYMDADVYHKSKDPIQGC
                     ADDITGFLKDFNSEEVDIAWAADNDGDPSAGTFDYRSPTGVLGVKLLDFSNPLAKISF
                     NWWKSSTEGFPEDWGPWEQASLNEWQTACPYGAPCSTFPGDGLGTPGGDKSKYFIMKN
                     QEIDYNQTETYMDHTAEGWLVPPGDAYDISYGMDTRFLLSFGPIDIPIGDSVYATLAI
                     VGGENFYTNPSNPSNRNFGDLVNNSNSALQAYLSWFTLPPLGPPKTFRLESSTDSTAT
                     LAWSSRKGNTNLKGYNIYRSTTPGIYNNPPINSTLIAVDSTSYTDCGLANGQIYYYTI
                     VCVNKNGLEGKKSDEVRVMVGRPKPPTGLQAKAEKDRVTLTWDSHPETHVTSFKIYRG
                     EFSGERTKIDSIGNLTSYVDTTVTNGVVYSYQITAIDTFGFESAPSESALAIPMAFDR
                     GIVVVDETRAKSLYDYHYDDSVNTFYQRALSGYNWFYLDQSDIGIQHADSLTLKELAP
                     YQVAIIHSEELLDNCPLGATKYYNNTYSVLDWYLKAGGKVIIEGRRNLTDENGECGYI
                     GGSGTECLRDSSELQPFIRENLHINQVFIPLWDSRNEEFIGAFSQIPEYPELQTDSIR
                     VEGSICPPFLYGIPLGGKLPLIGWFMPDDSAEVIYTFNSAYDTSQQEGKPVALRYLGD
                     TYKLVYFDFPLYFIKEDQATQLLHQALADLNQYTGVEDDDQESNIPSEFALAQNYPNP
                     FNAETAIEYSLVRNCQVKLVIYNILGQEVKTLVNEQQTVGRKKVIWDGKNQNGETVSS
                     GIYFYRIQAEGFVQSKKMLLLK"
     gene            complement(9171..14195)
                     /locus_tag="VGB16_01680"
     CDS             complement(9171..14195)
                     /locus_tag="VGB16_01680"
                     /inference="COORDINATES: protein motif:HMM:NF012311.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008236 - serine-type peptidase activity
                     [Evidence IEA];
                     GO_process: GO:0006508 - proteolysis [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="S8 family serine peptidase"
                     /protein_id="HEX9916423.1"
                     /translation="MKRQLCSFLFIFLFILLYFSQTSYSIYTRKIIKAKQTEPQFSPT
                     RLIVKLKPEVDKKVSLGKVGGKVVTGVAFLDTLNVRFKVKRQERLFGEFKKTALKVDR
                     LSSIYTLEVPQGTDLKSMKEEYEQRPEVEYVELDYKVELFEEPNDPLFAHQWYLNNLG
                     AAQNGGQGYYGINRPAGHTLVMKFGITDADIDALEAFQRTDEKVIPLVGIIDTGVDLD
                     HEDLASNIWVNPGEDLNGNGTVEPSEVNGLDDDHNGFVDDFYGWDFSGDEDNIIEDND
                     PTDTYGHGTHCAGIVAAVRDNSLGVSGINTPCRIMAIKFFPYAFMSLGAKAIIYAADM
                     GCDVINMSWGSPYPSSVVEEALDYAISKGVLPIAAAGNSGAEDNLYPAAYPQVFTIGA
                     SNSKDEVTYFSTYGEHIEVVAPGEDILSLRADNTDMYAPEYPLTHIINSKYYLADGTS
                     MASPCAVGVAAYILSASPGISNDSVISIMEQSADDIIYPYGGDSLYSPGKDIYSGYGR
                     VNLNSALELLSGRLAKIDYPYENAVVSGNIAIIGTASGANFVSYTLEYGEGYSPTTWT
                     EIISSTTPVTKDTLGMFITSGLSGLYTVRLTVGDQNQAVVHIMANSNAYVKITSPQED
                     DTIQGYTQISGYTIIPDFSYYTLEYGQGDEPSSWIPIDSSTKMVADYILGNWLVSFLV
                     EGTYTLRLTTHSNSEETYADSITLFVKSITSGGWIQDLPSYGSLSPAVGDIDGDGYDE
                     VVVGIGLQAGTGELGGVEVFSHNGQREAGWPKDTDKDMFSSPALGDLDNDGIDDIVIC
                     SNLGVHAYLFNSSNNWFRSAGTGANELFGLATPVIADLDNDGYAEVLTISSGGALYAW
                     YNDGESVIPVKNGVFAQTGIVNAEMNFPSLAVADLDKDGENEVIAGTANFTGSFGHYF
                     GEGGIYIWDENANPLLQPGDYPDTFSWVNGIAIADVDADGESEIIALGVTGNGNSFNT
                     LSAYKKDGTQASGFPIKIEGATAGWWFGNHPAVGDLDGDGTLEIVVTMWTPGEARIYA
                     WHQDGTPLVPQGPLVSIKSPNTDREKQVLSTLGNNIGEVIAKLKSMNKEELNSLVSTF
                     QDNVFASVTETFGNPILADVNEDGNVDIIVRAGYFVSSGYERVFAWDYEGNLTPGFPI
                     YASTNPSWLNYLPYSPVMADINKNGKLNMILATDWPDQKLVSWEFETNYDTTKTPWPK
                     YRQDKWNSARYGFSPPVGIPDLSPTDFHVKNWGESSVTLAWKPKPCVLNKGYNIYRST
                     TSGDYTQPPINSTLIPREDSLYEDTGLDENQTYYYVIANVDLNDNQSDFSKEVRFTLF
                     DTQILLIDLTKGRQENRVNGDSVNAFYGRALDGYGYTYVSPFDSSQDIYSSLGYLSNY
                     PLLIVHSEDFLIQWADLSLISPVLKEYFEAGGCLIIEGRRNLTDDNQSFGTRVFSPGS
                     FTFDCLGIDSAFIPLWDRYHRTEEFIGAHSLDGNYPEVVLDTARVNGNGYSGYVTVLK
                     GKLPGVGYFFPSDLSDMLYAFNSSDAGSFSNGQAVAIKRLGSESGIFYFDFPLFFVRE
                     DIATQILHQAISELEVYIDVNDEKEEKFVPTEFVLEQNYPNPFNSETVIRYALSQDCQ
                     VKVDIYDILGRKVITLIDEYQSRGFKRITWDGKDKNGNNVASGMYFCRIKAGSFAQAK
                     KMLLLK"
BASE COUNT         3969 a         3381 c         2718 g         4386 t
ORIGIN      
        1 gaaacaggta tagctttacg aaatcctttt gtcttatcac aaaaccggtc ttatctttga
       61 cgaatttctc aaacagggaa taaaccttat tcgggtttaa ctctcttttg gtgggtttct
      121 tggcggaact gttttttttc aaaattttat ataccgaagg gatatggttt atgcattcca
      181 cgtcaacata tcccaatttt ttatatccgg cgtgagccct tatagttcga ttagtgcaca
      241 gaaaagagaa acgatacccc ttttccctga aataccggtg agctttctcc atcaatatcc
      301 ctgctaagct tctgttaacg aaatcgggat tagtagccac tgcgctaata ccacccacaa
      361 cttccacttc tccctttaag gtcctcaatg gtatatccat cactcccaca tggctggcta
      421 gcctcccatt ctccattgcg cagaacccga ttgggctacc tttaagcctt tcatcccatt
      481 gcgtgattct gtcaaaatgc ctgggcacaa ccggccacca aaacgccatc tccataagca
      541 tcatgaattc atctttattt ttcaattccg gataggtgat tatctccatc tttaaccctg
      601 ccgatgtttc tggactatga agtcccgcct gaagcgagat ctacgacttt agctttattt
      661 tacaaacctg aaggtctgca caccaaaagt tctcaaaacc aatccagcac actcatatac
      721 agcgaatccc cgaaaacttc cctataatcc aaaccgtcca aagacttcac cataagcaca
      781 tggttggagc gcaattgaaa tttatatcct ctggacttat agacctccag gagaaccggg
      841 tcatatacat agtggtcgat aacagcccac tttgacatct gctctgcttt tattaccaac
      901 ttctcataag ttgacctatc cagagcaact atctcctgaa cctgcatagc gtcacttttc
      961 tcttgtaaga agatgtatcc ttttttcacc tgaacgaact tcttcttatc tataagctta
     1021 agaaatgagc ataactttac gaaatccttt tgcctgatga caaagccaac tttgtccttg
     1081 acgaattccc gaaacaagga gtaaatctta ttcgggtcca atttcttctt aatcgacttt
     1141 ttagcaccgt gtttgctttg tacgatttta tacacagttg gaatcctatt catagattcc
     1201 acttcaacat accctaacct cttgtaacca gcataagcga ttaaagttcg acgagtggac
     1261 agaaaagaaa aacgataacc cttttgcttg aaataagcat gagctttctc cattagtaag
     1321 cccgctatgc ccttattagc aaactccgga ttagtggcta ctgccctgat accacccaca
     1381 gtttccgtct tgccttctaa tgtttttagc gggatgttca atactcccac gtgacctgtc
     1441 aacttttcgt tctccatcgc acaaaatcct atggggctat ctttaaacca atggtccaat
     1501 ttgatgattc tgtcaatatc accaaggctg ataggccaac caaaagccat tctcattagc
     1561 atcatatatt catctttatt tttcaattcc ggataggtga ttatttccat ctttaaccct
     1621 gctggtgttt ttggagtgaa gaaccccacc cacagggtgg ggcttaaaaa tccaaaccga
     1681 gatcccgaac caagttcggg atgacaagaa aagaactaag ttcgggatga caaaaaccgt
     1741 aaaaccaaac cctgttgtca tgctgaacct gtttcagcat ctgatttcag catctggtct
     1801 gtttttttgc ttccggcaga cacacctttc atcgccgtcc acaggacggc gttttcaggt
     1861 gcgatcttaa taaagctcta tctttataat ttttggccca cactccaaag agcaactact
     1921 tctcgtcatt tgaacggata tttacatgaa tttttcgaac cgttctttct ttgccttaca
     1981 tataggacaa atctccggtg gctcgtttct ggcacacaga taaccacaca ccttacaacg
     2041 ccagactgga taggaaagag atgttttgct taaatcggat tctgaatttt tctctttcaa
     2101 tttttctctt ttatctttcg tgggccttct ttctggtatt gaactaactt tcttttttcc
     2161 atcgaacgca tcctttgata catacagagc gcagtagcag gtgccataat cattcaaatc
     2221 cgggtcgcgg taatcgcaag gacagattat atctaagtct tcccctttta tgcctgatgc
     2281 cagccgacag ggacaagccc agtagccgta tcttttttca ttaatcaaca aacccttcac
     2341 cagctctttg gtgaactcca catccgggtt caagtgataa ccgccgcttt caacttcttt
     2401 cttgagccta tcatacaact tgtctatctg ttgctggttt atatcaactt cctcattcat
     2461 aagcccagct tctccttaag ctcctctggt cggtagccaa ctatgcaatc cttgttgtct
     2521 atacccactg ttgggaagga aacggatggg ttccatctct gcatctccgc ttgggcttcc
     2581 tccttttcat cttcgcccaa aaggtcaaca tctacgtagt cataatccac ccccagctct
     2641 cccaaaaatt tcttcgtctt cttacaccac acacaggtgc ttatagcaaa cataagtata
     2701 tccccttttt tcgtgcccga tacatgcttt atatgttctt cgtaacccat attccctccc
     2761 tccttaggaa tcctagccgg attccgcctg tagccccgtt ttgggcgata aattttttag
     2821 aatttatttg atttatcgtt tacttatgct tgcttccccc ttagaggcaa tacgcctgcc
     2881 atttctccaa tcacattgac caattcagtg ccggcaggga taacgatttt ggcattatct
     2941 ttaagtgagt tctccacggc ttgaagtttt cttaagattt gcgcgttccc tacaaaatac
     3001 ttctccgctg cctcattcac caatcgaatg gcttcggctt caccttgcgc cgctaagatt
     3061 ttggcttgct ttataccctc tgccttttta atctctgccc ttcttgcccc atctgccacg
     3121 gtttcggttg cagtagcaaa atccaccgcc gcaaccttct cattctccgc tttgacgact
     3181 ttattcatcg tttcctgaac atctttgggt gggtcgatct cctttaattc tgttcgaacg
     3241 atatctatgc cccagctcgc agtctccgtc ataagtgttt tgtgcaactc gtcattgatc
     3301 ttaccgcgct cactgttggc ggacttcaaa gtgagtgtgc cgatgatgtt tctcaaagtc
     3361 gtgcgggcta aattcactat ttgccactgg tagttattta cattgtatag tgattttttt
     3421 acgctatcct catcattttt taccttgaaa tagacctgag catccacttt ggcattgaga
     3481 ttgtcaaaag ttatgatttc ctgcggctcc gcatcaacca tctgctcagt tatgttcact
     3541 tgatataact tctcaatacc gaaggggata atccaattga accctaatcc tgcaaagcgg
     3601 ttatacttgc ctaacctttc tatcaagccc ctgtgcgtgg gtctgacgat tctaatacca
     3661 ctaagaagta taataacgcc caccaaacaa attagaaaaa taagcatcta aaccctcctt
     3721 taataggtta taaaacaact ttttcagtag tctatgttat tcaacaaaat catgatatgc
     3781 atgttgccat agtttgttcc atactcagag gtttttcctc ctgctaagta tgccccaatt
     3841 tgatagtgtt tagcaaattc ataaccgcca cccataagat atccaaatcc gcgtgcgtgg
     3901 tcgggtggcg caggaggcat ttctccgccg ttaaggctga tccttatata agaactaaat
     3961 ggtggaagcc caaaatagta gcccccaatt ccaatcactg agaaaaaggt tctgctttct
     4021 tgcggaccaa agtaatgata ccaactaggt ccgaggaaaa actgaaggat gttgacatca
     4081 agaatatctg aattgtaaaa ggttccattg ccttcgagga caatcatgtt tttttcattc
     4141 catgcatagc cgattagagt gttatctccc cagcctacgc tattctcatt aaaaccacct
     4201 gttgaccatc tgttgaccgg agtaaaaccc aatcctccac ccagaacaaa acccttgcgc
     4261 tggccatcga aagccagagc tgaagtcgta aaaattaata aaaataacaa cagaaaaatc
     4321 aaaatccgtt tcatttttct tcttggctag ctatacgttt tcccctgtag tcccttgaca
     4381 ggggtatgct tcttctccct ccttaataag agggatttgc ttttctgtcc ccctgacaag
     4441 ggggaataca aagggggttt gacaacccac atcaaatcct gatacacacc ctcaatatta
     4501 ttcataacat ctcgattagt atatcgcaga accttcaatc catattggtt caaaacgctc
     4561 gttctggctt catcatattc cttgttttct acatgagagt caccgtcaat ttcaatcacc
     4621 aactgcaact ttgaacaata ataatcaaca atgaaattag taattggttt ttggcgcgtg
     4681 aacttgtatt cttcgaattg ttttcgacac aaaacttcat accatatttt cttttctgct
     4741 gggctggggt tgtccctgtt ttgttgtgcc aacttagtta gttttctttt gtaggggata
     4801 tagatgaatt tgtaaattgt ttttgttttt gccatttttt tgttttcatg gctttgttag
     4861 taacccccta aatccccctt atcaggggga ctttcctctt tttccccctg ttaaggggga
     4921 atacaagggg gttcaagtca aagaattaac agatcaagaa ttgcctccag acttacaaaa
     4981 ctgtgtctat ttttgtaata ctcagatttt cgctggcaat agctcgcaag gaaaatcata
     5041 aaagactttc ctctttggcg gcaaactctt ctaattttta atctttatgt tgctcaaaga
     5101 ctttaggtcc ttatcataaa agtcattgat ttctataaac caccgagaat gtgcttcttt
     5161 acttggttga caattaggat cgaaactaag gcgatcgcag agagcaaaca tcagcaaacc
     5221 tgcggtgact cctttcttcc aaacatcatc agcaaatagt cggtttagca ctcgagcaac
     5281 acatttcggg tcatgctctt ctttgtgaaa ctgcttaaat aaattcattc tcgttgtatc
     5341 aacacgggca aggtacgctc tatcaaatgg ttttttagat gttctgccaa gagtgctaga
     5401 acgagcaatt gcttgattat aggattccat agcatcccaa atgtcgtctc gttttttgtc
     5461 gtgcagataa tttttcgtga aagtactttg ggcaaccgct aatttatcac caaattggtg
     5521 cagccacgca agcgcaaata attcaaattg caaaactatt atttcgtcat taactttttg
     5581 ggaatcaatg ttggcgaaat tgtggtctgt ggctactatg gaatttttta atgtatctaa
     5641 aattgtagcg ttaacatcaa gcccattaac tacacaattc agaatgacat tgtcataaaa
     5701 aagacgacaa aatgcctcaa gttcaacttg ttgtttttta ttaaaaagtg acatatgttt
     5761 cccttttact ttattaactc ctctactaaa acaccgagcg ctttggtgat tttccccgtg
     5821 gtctcaacgc ttggtttatt aacaccactt tcgactttgc gggcgaacta aatttcactt
     5881 ttacgagcta ccggacagtt cggcagtcga aaccacctct ttccgcctaa cttatttatt
     5941 tcgacaaaat tagccaaagt ttgttgcctt gtcaagggtt aagttgcgtc aagttgcgtc
     6001 aagttccttc aagttggatg aagtccattg ttagaaatgg agagtctctg tagctgataa
     6061 gactaagtga tcagcgttta gttgggaagt cacaaaatca cctgatcaac cacagacaaa
     6121 aagccacctt tttaggaggt ggcttctact aaaatctgac cccaattcaa aactacttta
     6181 acaacagcat cttcttcgac tggacaaacc cttccgcctg tatcctgtag aaatagattc
     6241 cacttgagac tgtttctcca ttttggttct tgccatccca gattactttt ttgcgtccga
     6301 ctgtttgctg ttcattaacc aaggtcttta cttcctgacc caggatgttg tagataacca
     6361 gcttgacctg gcaattcctg acaagggagt attctatagc agtctcggca ttaaatggat
     6421 tgggatagtt ctgcgctaaa gcgaattcgg atggaatatt gctttcttga tcatcatctt
     6481 ctactccagt atattgattc aaatctgcta aggcttgatg cagaagttgc gtagcttggt
     6541 cttctttgat gaagtataaa ggaaagtcaa aatagaccag cttgtaagta tctcccaaat
     6601 atcttaaggc aacaggcttt ccttcttgtt gggaagtatc ataagctgaa ttgaaggtat
     6661 agatgacctc agctgagtca tccggcataa accagccaat gagagggagt ttgccaccca
     6721 agggaatacc atagagaaaa ggagggcaga ttgacccttc aaccctgatg ctatcagtct
     6781 gtaattcagg atattcaggt atttgactga aagccccaat gaactcctcg tttctactat
     6841 cccacaatgg aataaagact tgattgatgt gtagattctc tcgaatgaat ggctgaagct
     6901 cagaagaatc tctcagacac tccgtcccag agccaccaat ataaccacat tccccatttt
     6961 catctgttag gtttcttctt ccctctatga taactttgcc tcctgctttt aaataccaat
     7021 ctaaaaccga gtaagtattg ttataatatt tagtagcacc taaaggacaa ttatcaagta
     7081 attcctcaga atggataata gcaacttggt aaggggcgag ttcttttaag gtcagggaat
     7141 cggcgtgttg tatgcctata tcactctggt caagataaaa ccaattatat ccagaaagtg
     7201 ctctttgata aaaggtattc acgctgtcat cgtagtgata gtcataaagg cttttagcgc
     7261 gcgtctcatc cactaccaca attcctcggt caaaagccat gggtatggca agggcacttt
     7321 cggaaggcgc actctcaaag ccaaaagtat caatcgcagt tatctggtat gaatagacca
     7381 caccgttggt tacggttgta tcgacataag aagtcagatt tccaatcgaa tcgatttttg
     7441 tcctttcacc agagaactct cctcgatata tcttaaaact tgttacgtgt gtttcaggat
     7501 gggaatccca agttaacgtt actcggtctt tctctgcttt agcttgcagt cccgtaggag
     7561 gttttggcct accaaccata acccgtacct catctgattt tttgccttcg agaccattct
     7621 tgttcacgca gacaatagta tagtagtaaa tctgcccatt ggccagaccg caatcagtat
     7681 atgaggtgga atcaaccgca atcagggtgc tgtttatcgg tggattgtta tatattccgg
     7741 gagttgtgct tctgtaaata ttgtatcctt tcaaattggt attacccttt ctcgaagacc
     7801 aggctaaagt tgcggtcgaa tcagttgagc tctccaaacg gaatgttttt ggtggcccaa
     7861 gaggtggtag ggtaaaccag ctaagataag cctgaagagc actattagaa ttgttgacca
     7921 aatccccaaa attacgatta gatggattgc ttggattggt ataaaagttc tccccaccca
     7981 ctattgccag agtagcgtaa actgaatccc cgattggaat atcaatagga ccaaaggaaa
     8041 gaagaaatct tgtatccatg ccgtatgaaa tatcgtaagc atctccgggt gggacaagcc
     8101 agccctctgc agtgtgatcc atatatgttt cagtttggtt ataatctatt tcttggtttt
     8161 tcattataaa gtatttagat ttatctccgc caggagttcc caaaccatca cctggaaagg
     8221 tgctacacgg agccccataa gggcaagcgg tttgccattc atttaaactg gcttgttccc
     8281 agggtcccca gtcttccgga aagccctcgg tgctactttt ccaccagttg aatgaaatct
     8341 tagcaagtgg gttagagaaa tccaacagct taacccccaa cactccggta gggctgcgat
     8401 aatcaaatgt acctgcagaa gggtccccat cattgtccgc tgcccaggca atatccactt
     8461 cttcactatt aaaatctttc aagaagccgg tgatatcatc tgcgcatcct tgaatgggat
     8521 ctttgctctt atggtaaaca tccgcatcca tatacatccc caagtaagcc ccagaaatat
     8581 gctttccact tatgtttttt atcttgtaat ccaagataac aaaatcatca tagggtggag
     8641 taacccagga ataagtatgc aaggttatct ccaatccaat tggcttgtgc ggacggccat
     8701 cataggggtc gtcattttca gtaggagtta cggtatcagt gaagacagca atgaactcct
     8761 ggtcagctgt ggagtcaatt tccctgatga aatagtcagg gtcaggatag ggcttcttgg
     8821 gtgagaactc ataaattggc tcccatccat ccatagaggt cgaagtcaaa gtctcactct
     8881 caactactgc actaacccaa aagccactca tataaagata ttctatacct gagcctttag
     8941 gaaactcgca ggagagaaag ggtttctggg ttcccgatgc agatgtccct aaccaaccac
     9001 tgttgctgat agctaaagcc agattacctt gattgtgaac acgataatca gcagtaagct
     9061 gctgggcttc agtctctgtg aattgcttgg aatgtccatc ttggaatacg aaaagaccca
     9121 ttataactac tacaaaaaat agtatcgctt ttttcatctc tgcctccctt ttatttcaat
     9181 agtaacatct tttttgcttg tgcaaaactg cccgccttta ttctacaaaa gtacattccg
     9241 cttgccacgt tatttccatt tttatccttt ccgtcccagg ttatcctctt aaaccctctg
     9301 ctttgatatt catctattag ggttatcacc ttgcggccaa gtatgtcata gatatctacc
     9361 ttaacctggc aatcttggga taaagcatac cggataacag tctctgaatt gaaagggtta
     9421 gggtagtttt gttccaatac gaattctgtg ggaacgaact tttcttcctt ttcgtcattc
     9481 acatcaatgt atacctccag ttcgctaata gcttgatgta aaatctgggt ggcaatatcc
     9541 tctcttacaa aaaacagcgg gaaatcaaag tagaatatgc cactttcgct acctaagcgt
     9601 ttaatcgcta ctgcttgacc attggagaag gaaccggcat cggaggagtt aaaggcatag
     9661 agcatatctg ataaatcaga gggaaagaaa tatcccacgc cgggaagctt tccctttaaa
     9721 accgttacat atccactata gccattaccg ttgacccgag cggtatctaa aacaacttca
     9781 ggataattcc catctaagct gtgtgctccg atgaattcct cggtgcggtg gtatctatcc
     9841 cagaggggta tgaaagctga atcaataccc aaacaatcaa aggtaaaact gcccggggag
     9901 aatacccttg tgccgaaaga ctgattatca tctgtcaagt tccttctccc ttcgataatt
     9961 aagcaacccc cagcttcaaa gtactctttc aggactgggg atatcaaact aaggtcagcc
    10021 cactggatta gaaaatcctc agagtggacg atgagtaagg ggtaattact tagataaccc
    10081 agagatgaat agatatcctg actggaatcg aatggactaa cataggtata gccgtatcca
    10141 tctaaggccc ttccgtaaaa ggcgttcacg ctatcgccat tcacacggtt ttcctgccta
    10201 cctttggtta aatctatcag taggatttgt gtgtcaaaga gcgtaaaacg gacttccttg
    10261 gagaaatcac tttggttgtc atttaaatca acattggcaa taacataata gtaggtttga
    10321 ttctcatcta accccgtatc ctcatataga ctatcttctc gtggtatgag ggttgagtta
    10381 atcggaggct gggtataatc tcctgaggta gtactcctgt agatattgta tcctttgttc
    10441 aacacgcaag gttttggctt ccaggccaaa gtcacactgc tttcgcccca attctttaca
    10501 tgaaaatcag ttggtgagag atctggtatt cccacgggtg ggctaaaccc atatcttgca
    10561 ctattccatt tatcttgcct atacttgggc cagggggtct tggtggtgtc ataattggtc
    10621 tcaaactccc aagagactaa tttttggtca ggccaatctg tagccagaat catatttaac
    10681 ttgccgttct tatttatgtc agccattacc ggagaataag gaaggtagtt tagccaggat
    10741 ggattagttg aggcatagat aggaaaaccc ggtgtgagat ttccctcata atcccaggca
    10801 aagactcttt cataaccact gctcacgaaa taccctgctc gaactatgat atccacatta
    10861 ccatcttcat tcacatcagc taagattggg tttccaaagg tctcagttac agaggcaaag
    10921 acattatctt ggaaagttga aactaaagag ttaagctcct ctttattcat actcttgagc
    10981 ttagctatta cttctccaat gttattgcct aaggtagata aaacctgttt ttctctgtca
    11041 gtattaggtg atttgatgga gactaaagga ccttgtggaa ccagcggtgt tccatcctgg
    11101 tgccaggcat agatgcgggc ttcacccggt gtccacatag ttaccactat ctccagagtg
    11161 ccatctccat ctaagtctcc aactgctgga tgattcccaa accaccagcc agctgttgcg
    11221 ccttcaatct tgatggggaa gccactggct tgagtcccat ccttcttgta agcggagaga
    11281 gtattgaagc tattgccatt tccagttact cctaacgcta ttatttccga ctctccatct
    11341 gcatccacat cggcaatggc aattccattt acccaggaaa acgtatcagg atagtctccg
    11401 ggctgaagca gcgggttggc gttttcatcc cagatgtaga ttccaccttc accaaagtaa
    11461 tgtccaaaac ttccagtaaa attagctgtc ccagcaataa cctcgttttc tccatcttta
    11521 tctaagtcag ctacggccaa ggagggaaag ttcatctcag cgtttacgat ccctgtttgg
    11581 gcaaagactc cattcttgac aggaattact gactcaccat cattgtacca agcatagaga
    11641 gcgcctccac tacttatggt caggacttct gcataaccat cattgtcaag atcagctata
    11701 actggtgtgg ctaagccaaa aagttcgttt gctccagtac ctgcacttct gaaccagtta
    11761 ttagaagagt tgaagagata agcatgaact cccagattgg agcagatgac tatatcatca
    11821 ataccatcat tatccaagtc tcccaaagca ggtgaggaaa acatatcctt gtcagtatcc
    11881 ttaggccagc cagcttctct ttgaccattg tgggaaaaga cctcaactcc tcctagttct
    11941 ccggttcccg cttgcagacc aatccccacc accacctcat catatccatc tccatctata
    12001 tctcccactg caggggagag ggagccataa cttggaaggt cttggatcca cccaccagag
    12061 gtgatgctct tcacaaataa ggttatgctg tcagcatagg tctcttcgct atttgaatgt
    12121 gtagttaacc ttaaggtgta ggtgccttca accaagaagc tcactaacca gttccccagg
    12181 atgtagtctg ccaccatctt ggtggaacta tctataggta tccaactaga aggttcgtct
    12241 ccttgtccgt attctaaggt ataataagag aagtcaggga taatggtata accagagatt
    12301 tgagtatatc cttgaatagt atcatcctct tgaggtgagg ttatcttcac ataggcgtta
    12361 ctattggcca tgatatggac cactgcttgg ttttggtctc ctacagtaag tcttacggta
    12421 taaagtccag atagtccaga ggtgataaac atccccaaag tatctttggt tactggagta
    12481 gtggagctta tgatttctgt ccaagttgta ggtgaataac cttctccata ctctaaggta
    12541 tagctcacaa agtttgctcc actggctgtt cctatgatag caatattccc tgaaaccact
    12601 gcattctcat aaggataatc tatcttagct aatcttccgc taagtagctc taaggcggag
    12661 ttaagattta ctctaccata accactgtag atgtcctttc caggagaata taaactatct
    12721 cctccataag gatagataat atcatctgcg gactgttcca ttatgcttat tacactatcg
    12781 tttgatatcc caggtgaagc tgacaagata taagctgcca ctcccactgc acagggggaa
    12841 gccatgctgg tgccatcagc aaggtaatat ttagagttta tgatatgggt aagtgggtat
    12901 tcgggagcat acatatccgt attatctgcc ctcaaagaga gtatatcttc acctggagct
    12961 actacctcta tatgttcccc ataggttgag aagtaagtta cctcatcttt actattggaa
    13021 gctcctatgg tgaagacctg gggataagca gcaggataga ggttatcttc tgctccacta
    13081 tttcctgcag ctgctatagg cagaactcct ttagagatag cataatctaa tgcctcttct
    13141 accacagatg aaggataagg gctaccccaa ctcatattaa ttacatcaca tcccatatct
    13201 gctgcataga tgattgcttt tgctcccagg gacatgaatg cataggggaa aaatttaatt
    13261 gccattatcc tgcaaggggt atttatgcct gagactccga gactattgtc tcttaccgca
    13321 gcaacaatac cagcacagtg agttccatga ccataggtat cagtggggtc gttatcctca
    13381 attatattat cctcatctcc tgagaagtcc caaccataga aatcatccac aaatccatta
    13441 tggtcgtcat ctaaaccatt aacttcagat ggttctactg taccattacc atttaaatcc
    13501 tctccgggat taacccagat gttactagcc aagtcctcat ggtctaaatc aactccagta
    13561 tctattatcc ccactaaggg aataaccttc tcgtctgttc tctggaatgc ttctaaagcg
    13621 tctatatctg catctgtgat tccgaacttc ataactaacg tatgtcctgc aggtctgttg
    13681 attccataat atccttgacc accattctgt gctgctccca agttatttag ataccactgg
    13741 tgagcaaaga gaggatcgtt gggctcctca aatagctcta ctttataatc aagctccaca
    13801 tactccactt caggtctttg ttcatactct tccttcatgc tttttaagtc tgtcccttgt
    13861 ggaacttcca gggtatagat actggagagt ctatctactt ttaaggcagt ctttttaaac
    13921 tcaccaaaca gtctttcctg tcttttaact ttaaatctaa cattaagagt atccagaaaa
    13981 gcaactccag ttaccacctt tcccccaacc ttacctaaac ttactttctt atccacttct
    14041 ggctttaact tcacaatcag tcgggtggga gagaactggg gttcagtctg ttttgcctta
    14101 attatcttgc gtgtgtagat ggagtaggag gtttgggaga aataaagaag gataaaaagg
    14161 aagataaaaa gaaaagagca gagttgcctt ttcataagaa cgcccctttt gtttgaactc
    14221 attagttatg atatcgactt tttgatgcct cctcttaaga gaaaaagcaa aagggatgcc
    14281 aaatagtcaa agccaaatgg ttagatcctc ctctattcaa gttcgttgag tacaaacgaa
    14341 atagggtaaa catcaatagg ttagaagact tcgccagaaa tggcggatat tggagggaat
    14401 ggtgaggggt aattaccgct tttcttttga aaacgcaaaa gatagtaaaa caac
//
LOCUS       DASWLN010000042        13163 bp    DNA     linear   ENV 20-FEB-2024
DEFINITION  MAG TPA_asm: Candidatus Zixiibacteriota bacterium isolate
            SMAG_U2949 k141_553041, whole genome shotgun sequence.
ACCESSION   DASWLN010000042 DASWLN010000000
VERSION     DASWLN010000042.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37960594
            Sequence Read Archive: SRR10376091
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Candidatus Zixiibacteriota bacterium (soil metagenome)
  ORGANISM  Candidatus Zixiibacteriota bacterium
            Bacteria.
REFERENCE   1  (bases 1 to 13163)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 13163)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (26-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/12/2024 08:28:26
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 1,440
            CDSs (total)                      :: 1,416
            Genes (coding)                    :: 1,412
            CDSs (with protein)               :: 1,412
            Genes (RNA)                       :: 24
            rRNAs                             :: 1 (16S)
            partial rRNAs                     :: 1 (16S)
            tRNAs                             :: 21
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 4
            CDSs (without protein)            :: 4
            Pseudo Genes (ambiguous residues) :: 0 of 4
            Pseudo Genes (frameshifted)       :: 0 of 4
            Pseudo Genes (incomplete)         :: 3 of 4
            Pseudo Genes (internal stop)      :: 1 of 4
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..13163
                     /organism="Candidatus Zixiibacteriota bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_553041"
                     /isolate="SMAG_U2949"
                     /isolation_source="soil"
                     /db_xref="taxon:2053527"
                     /environmental_sample
                     /country="USA: Oregon"
                     /lat_lon="42.99 N 123.63 W"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            complement(<1..3377)
                     /locus_tag="VGB16_01685"
     CDS             complement(<1..3377)
                     /locus_tag="VGB16_01685"
                     /inference="COORDINATES: protein motif:HMM:NF013957.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004866 - endopeptidase inhibitor activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="MG2 domain-containing protein"
                     /protein_id="HEX9916424.1"
                     /translation="MIWKSIILGLSLVFFLQGVGAASKLKEADELFDKGTFQEALKIY
                     ESVYKETSDSQTCEKAFFRICESLAHLFRYGEAAQKLFSTEVPRQLPQYPRFLILKAE
                     IFRNFLMQYSYLQRGDKIDEYGKEVFRLTPNEIRNEIRKAYIELWELRGELVKMDIRG
                     EGYFLDIDNIDFGMYPTFFDYLIFSWTDFLLRTEASYVVENAVCPGAELLLVEKFKRS
                     VDLNGPPALLAAELMEEASRFGGTGRLEANERWKIRRLLLPLRYSNLFNLKGLAEDKT
                     LYDGKDLRVCRDHTKAILFRWMDVFETREAKAEAGYEAAMILNSDNKPAEVVTLCERI
                     ERTFPGTHASRHAQALRSQIQMPTLYLVAKTVLPPAQGAFTITTKNLKKVYFRIYRIN
                     PEELKGRYINYRKEEYGNSYDSFDGWSGVLNLNWSYRDWGKKLLESYLSRKNACKEWS
                     TQTGDKSNYETLVKTIDPPALEAGMYLVFVCGDGSFKIGSSLLSACFLNVTDLVLLGT
                     AGFTTNAIDAYHDFIDDRGPDHITDNAFHFYTLDAKTGRPINGAKLDVYVYLSHQSKR
                     ESSGLRTNKEGSVSLSLPLVISPFAQNYYYADPLAQRDGSFAYWRQNQSLNYYPPSPL
                     VLFTETDRPIYKPGDKVEAKVVVVRRIPQGFRTFDKTQKVIFSARDPNAKEFFNQTVK
                     LGEFGSASVNFEIPGGRLLGRYSLNVSCQDGRFNNNASIFFSVEEYKQPEFEIEFKTA
                     EEPWKYGQPGEIKGEVTYYFGGPVPDAPITYHIKRQTYVPYCYRWWFGESHSGSGYEV
                     ATGQTKTDSKGNFVIPFTPEAVPGSYAGYVPDISQFSVELEAHDTGGRTVEAQQSYRV
                     GKNAIYLVIEPEKGFYLENETIEIKSKLLTINDAPAQGASSYEVFSLKDTTTKSLADI
                     GYGYYGGYWSWIPPLDVQLKDVPNEKLLAQGKIKHDKDGKGIIRIRPLHQGAYRIVEK
                     TPDKWGGEVAQTKILVVVKDTKTVIPVNAASVTLVEKDEYKIGEVARFVIGSGLASGI
                     YHIELWAGEHLLSHQLIDGSQPVRLIEVPVTGRMKGGFTLRWFGVKDFDVHYGQATVL
                     VPWNEKKIKVSLKPFNKELKPDQEYSWGVRLTDAKGWP"
     gene            complement(4476..4724)
                     /locus_tag="VGB16_01690"
     CDS             complement(4476..4724)
                     /locus_tag="VGB16_01690"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_008411419.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0015036 - disulfide oxidoreductase
                     activity [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="thioredoxin family protein"
                     /protein_id="HEX9916425.1"
                     /translation="MDIKILGPGCSNCKKLEQNVRDALTELKIEANVEKITDISKFAS
                     YGIMMTPGLVINGKVYSSGKTPVMATLKNWIEKESKGI"
     gene            complement(4745..5434)
                     /locus_tag="VGB16_01695"
     CDS             complement(4745..5434)
                     /locus_tag="VGB16_01695"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_015720705.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0016020 - membrane [Evidence IEA];
                     GO_process: GO:0017004 - cytochrome complex assembly
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="cytochrome c biogenesis protein CcdA"
                     /protein_id="HEX9916426.1"
                     /translation="MESLLGNISGVINNAPFLAYILIFLGGILSASSPCVLAIIPLVI
                     GFVGGYSKGDKKKALLYSLTFALGLSITFTALGVVASLLGRLFGDVGKLWYFVAAGVA
                     LIMGLYLVGAIKISFPKTVDLKTKHKGVLGAFLLGLLFGMVSSPCATPILAVILAFVA
                     TKARIMYGVSLLFVYALGHCALIIAAGVSAGFVESFAQSRGITNFSNLAKKVSGLLII
                     FAGIYILWINL"
     gene            complement(5435..5872)
                     /locus_tag="VGB16_01700"
     CDS             complement(5435..5872)
                     /locus_tag="VGB16_01700"
                     /inference="COORDINATES: protein motif:HMM:NF012314.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="thioredoxin family protein"
                     /protein_id="HEX9916427.1"
                     /translation="MKNRTILLVILAITILIVGLFILKQKNEIGLGNKPASLSESAVL
                     SENSSDTLNKNLPALIDLGRGTCKACKMMQPILEELSEEYKGRATIRIVDIRYEPEQA
                     NFYNIRLIPTQIFFDSAGNEVYRHEGFMDKLSIQQKLLEIGVK"
     gene            complement(5913..6431)
                     /locus_tag="VGB16_01705"
     CDS             complement(5913..6431)
                     /locus_tag="VGB16_01705"
                     /inference="COORDINATES: protein motif:HMM:NF040494.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="nitrophenyl compound nitroreductase subunit ArsF
                     family protein"
                     /protein_id="HEX9916428.1"
                     /translation="MNKKSPVFCLVYVLVFLMMYTSGHSQNENRQTPLKKNVNDASKK
                     VVDSTLSFNFDSLLVIHFHPTVQCSCCINAGSFAKEGLIKFYTKPYEDGHIIFKEYNI
                     DKDSLIAKRYKIFWSALGFQKFSGKDNTFKEIESVWQFCEEKDKFLSNFQEQLETFIA
                     DKENKKVKNTAK"
     gene            complement(6515..7603)
                     /locus_tag="VGB16_01710"
     CDS             complement(6515..7603)
                     /locus_tag="VGB16_01710"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_013560510.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="permease"
                     /protein_id="HEX9916429.1"
                     /translation="MTFSQILLSGWNALLEYLSAHVLTCLVPAFFIAGAISALLPKEH
                     ITKHLGPEAPRPKAYLFSVMAGLLLAVCSCTVLPLFAGIKKKGAGIGPAIAFLYTAPA
                     TNILAILFTGSVLGWDFAFSRIVLSIGFAVLTGIIISGLFPHEEENCEPVESLLEEQK
                     NLEGIKKMSLRQKIDELVRKKLLVFFVTLFAILIFGASKIEGGIKIPVLLGLIIVLLL
                     QLKFRFQKEEILSWLTETYNFFKMIFPLLLIGIFVAGMLGAIFPRNYLGRYVGTNTLG
                     SNLLAVLFGLFMYFPTLVEVPMAKMFLGLGVAKGPLLAYLLADPVISLPSILVVRKIM
                     GSKRTLVYMILITAFCTLSGLIYGVFVK"
     gene            complement(7616..8671)
                     /gene="arsB"
                     /locus_tag="VGB16_01715"
     CDS             complement(7616..8671)
                     /gene="arsB"
                     /locus_tag="VGB16_01715"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011288365.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008490 - arsenite secondary active
                     transmembrane transporter activity [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="ACR3 family arsenite efflux transporter"
                     /protein_id="HEX9916430.1"
                     /translation="MADIGKKLSCFDRLLTLWIFLAMCLGVGAGYFIPGIDSFINRFQ
                     VGTTNIPIAIGLILMMYPPLAKVKYEELGDVFRNWKVLGISLVQNWIIGPVLMFILAI
                     VFLRGYPEYMVGLILIGLARCIAMVIVWNELAKGDTEYAAGLVAFNSIFQVLFYSLYA
                     YVFITVLPVWFGLKGSAVDVSMRQIAESVFIYLGIPFIAGIITRFVLIKLKSKEWYHS
                     KFIPKISPITLIALLFTIVVMFSLKGEYIVKIPLDVVRIAIPLCIYFVVMFLLSFWMG
                     KKIGANYAKAATIAFTAASNNFELAIAVAVAVFGIKSGVAFAAVIGPLVEVPVLISLV
                     SLALWFQKRYFSLEKSK"
     gene            complement(8664..9047)
                     /locus_tag="VGB16_01720"
     CDS             complement(8664..9047)
                     /locus_tag="VGB16_01720"
                     /inference="COORDINATES: protein motif:HMM:NF033788.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003677 - DNA binding [Evidence IEA];
                     GO_function: GO:0003700 - DNA-binding transcription factor
                     activity [Evidence IEA];
                     GO_process: GO:0006355 - regulation of DNA-templated
                     transcription [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="metalloregulator ArsR/SmtB family transcription
                     factor"
                     /protein_id="HEX9916431.1"
                     /translation="MQQPAKVLKALGDETRLRILKLLLKGKFCVCELEAALDLPQSKV
                     SRHLTVLRSVDLAEDSREGLWIFYSLFKPKNDFERAIFQVIENALSDNHLIKKDQERL
                     RRKLSQVYGYKCKQSKSRKGALCHG"
     gene            complement(9427..9939)
                     /locus_tag="VGB16_01725"
     CDS             complement(9427..9939)
                     /locus_tag="VGB16_01725"
                     /inference="COORDINATES: protein motif:HMM:NF014912.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ferritin family protein"
                     /protein_id="HEX9916432.1"
                     /translation="MQKEDVLEILKRGIKTEIDGFQLYKMASEKTKDPKAKDVFKLLA
                     EDELKHEKMLREQYKKFKENGKFKFEKKDKGSKLDTVSSYPIFSENFRGKLLKEQSFE
                     MSALSIGIMLEQNSIDFYKNSSEKIGDLDAKALFNFLAEWEGEHLKALVNQQRVLQED
                     FWTEARFYPF"
     gene            complement(9949..10407)
                     /locus_tag="VGB16_01730"
     CDS             complement(9949..10407)
                     /locus_tag="VGB16_01730"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_018194389.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="peroxiredoxin"
                     /protein_id="HEX9916433.1"
                     /translation="MPIKVGEKAPDFVLKGGDGKEYKLSDYKGKNVFLAFYPADFSPV
                     CSQEHACFINDLKRFESLNAVIFGISVDSVWSHKAFAEKLKLTYPLLSDFHRKGEVAS
                     RYGLYLKDLGITNRATVIIDKQGIVQYVKVYDIPQARDNQELIEQLEKLK"
     gene            complement(10878..12644)
                     /locus_tag="VGB16_01735"
     CDS             complement(10878..12644)
                     /locus_tag="VGB16_01735"
                     /EC_number="4.1.1.49"
                     /inference="COORDINATES: protein motif:HMM:NF013461.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004612 - phosphoenolpyruvate
                     carboxykinase (ATP) activity [Evidence IEA];
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_process: GO:0006094 - gluconeogenesis [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="phosphoenolpyruvate carboxykinase (ATP)"
                     /protein_id="HEX9916434.1"
                     /translation="MSLKPKPYNVKYIVDPTQEQLRELALKNTPAIMKTKYGNIDKFS
                     RYKARMAKFTYCIAPESEKSNFSGSIIDPAKAQQLIEKQRAYIEKKGTLIEINAYYGY
                     GKGAVPVQWFYTPEAANIAGMQQVLAFSRSAVENKNQLKEPFEPKFRLVYTAGLVLED
                     MPGKLAIVVDLNNWTTYVMGSDYFGETKKGVLRMLNEYVYRLGGLVLHAGAKKVTING
                     KSMTMGILGLSGTGKTTTTFSKQGELAQPIQDDMIVMQPDGTLTITENGCFAKTYGLT
                     VESEPVLYAGTIHKDAWVENVFPNVDGTYDFSKARLTPQEVGRLRDMLVQSGADPKNV
                     DDFISGKVKIDEVVDEYDIPKDGWDFTVWSQNGRSIIPMKAIKDAASFDNIPPLRSLG
                     ILNRDEGKDAATPGIVRFSTPQLAAGYFMLGETTKTSAAGKERGKTRSPFTQPFFTSP
                     HGLQAKRFSELAAKLPGLETWMMNTGYIGGESRDEKGGKALKVKIPHSSAMLQAMLSG
                     NIKWKKDPDFGYEIVDVDAKENAELLKKVPKEILNPIVFFQKNGRMEEYKAWVENMKK
                     EREAFLNKFNVDKKIVDAVVNR"
     gene            12916..>13163
                     /locus_tag="VGB16_01740"
     CDS             12916..>13163
                     /locus_tag="VGB16_01740"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEX9916435.1"
                     /translation="MPMKEWFAEAEGHKIRVENTWTGGTRLYIDGECRDRNTGWFAAS
                     KTRWLSAPLKPGDPKSATVEVFVKALFTVKAQICVAGKP"
BASE COUNT         3749 a         3039 c         2559 g         3816 t
ORIGIN      
        1 ggccagcctt ttgcgtcagt cagccttact ccccagctat attcttgatc cggcttcaac
       61 tctttattaa agggttttag actgactttt atttttttct cgttccaagg aaccaaaacc
      121 gttgcctgtc catagtgaac gtcaaaatcc tttaccccaa accagcgcaa ggtaaaaccg
      181 cctttcattc ttcctgttac aggaacttcg attaatctta caggttgact accatcaatg
      241 agctggtgac tcaacaggtg ctctcctgcc cagagctcaa tatgatatat accagaagcc
      301 aaccctgaac caataacgaa tctggctacc tctcctattt tgtactcatc tttttccacg
      361 agggtcaccg aagcagcgtt tacgggtatc actgttttgg tatcttttac gacgacaagg
      421 atcttagttt gtgctacctc tcctccccac ttgtctggag ttttctccac aattctatat
      481 gctccttggt gcaaaggtct aattcttatt atgcctttgc catctttatc gtgcttaatt
      541 tttccttgag cgagcaactt ttcatttggg acatccttta actgaacatc taagggtggt
      601 atccaactcc agtatcctcc gtagtaccca tatccgatat cagccagcga ttttgttgta
      661 gtatccttca aactaaaaac ctcataactg cttgctcctt gcgcaggggc atcattgatg
      721 gtcagcagtt ttgacttgat ctctattgtt tcattctcta aataaaaccc cttttctggt
      781 tcaatcacaa gataaatggc attttttcct actctgtaag attgctgtgc ttcaacggtt
      841 cttccaccgg tatcgtgtgc ctcaagttca actgaaaatt gtgaaatgtc aggaacataa
      901 cctgcgtatg acccaggaac agcttccgga gtaaagggaa taacaaaatt tcccttagag
      961 tcagtttttg tctgacccgt ggctacctca taaccactgc ctgaatgact ttccccaaac
     1021 caccaacgat agcagtaagg tacgtatgtc tggcgtttga tgtggtaagt aatgggcgca
     1081 tctggaacag gtccgccgaa ataatacgta acttcccctt tgatctcgcc tggttggcca
     1141 tacttccaag gttcttcagc ggttttaaac tcgatctcaa actcaggttg tttatattcc
     1201 tctactgaaa agaaaatgct tgcattatta ttaaatcggc catcctgaca ggaaacattg
     1261 agggaatatc taccgagtaa cctaccgcca gggatctcaa aatttacact ggcgctacca
     1321 aactcaccga gtttgacggt ttggttaaaa aactctttag cattgggatc gcgggctgaa
     1381 aaaattactt tttgtgtttt atcaaaagtt ctaaaccctt gaggtatcct cctcaccact
     1441 accacttttg cttctacctt atcccccggt ttatatattg gtctatctgt ctcagtgaaa
     1501 agcacaagtg ggcttggtgg atagtaatta aggctctgat tctgcctcca ataggcaaat
     1561 gagccatctc tttgggccag agggtcagcg taataataat tctgggcgaa aggtgaaatt
     1621 acgagcggca aggacaagga aacggatccc tctttattgg tcctaaggcc agaagactct
     1681 ctttttgact ggtgagataa gtagacatac acgtctaact tagctccatt aataggtctt
     1741 cccgttttag catccaaagt gtaaaaatgg aaggcattat cagttatgtg gtctggaccg
     1801 cggtcatcta tgaaatcatg gtaggcatca atagcgttgg tggtgaatcc cgccgtgccc
     1861 agaagaacaa ggtcagtaac attaagaaaa caagcggaga gaagagaact cccaatttta
     1921 aaagacccat ctccacaaac aaaaaccaga tacatacccg cttctagtgc tggtggatct
     1981 attgtcttga caagtgtctc ataattgctc ttgtcaccgg tttgggtgct ccattctttg
     2041 caagcatttt ttcttgacaa gtacgattca agcaacttct tgccccaatc ccgataagac
     2101 cagttaaggt tcaacacacc agaccaacca tcaaaactgt cataggaatt accatattct
     2161 tctttacgat aattaatgta tcgacccttg agctcctctg ggtttatgcg gtaaatacga
     2221 aaatacactt ttttgagatt ttttgtagtt atagtaaagg cgccctgtgc cgggggtaag
     2281 acggtttttg ctacaagata aagagtgggc atttgaattt gagaacgcag agcttgggca
     2341 tgacggctag cgtgtgttcc gggaaaagtt ctctctattc tttcacatag tgttactacc
     2401 tcagcgggtt tgttatcaga attcaatatc attgctgcct catacccagc ctctgccttt
     2461 gcctctcttg tttcaaaaac atccatccac ctaaatagaa ttgctttggt gtgatctcta
     2521 cacacgcgga gatctttgcc atcatatagg gttttgtctt ctgccaagcc ttttaggtta
     2581 aatagattgg agtatcttaa aggaagcagt aatcgcctta tcttccagcg ttcatttgcc
     2641 tcgaggcgac ccgttccgcc aaagcgactt gcctcctcca taagttctgc tgccagtaaa
     2701 gccggcggac catttaaatc gactgaacgc ttgaatttct cgactaaaag taactcggca
     2761 ccaggacaga cagcattctc aactacgtac gaagcttctg ttcttagaag aaaatcggtc
     2821 cagctaaaaa tcaaataatc aaagaaagtc ggatacatgc caaaatcgat attatctatg
     2881 tccagaaaat atccttctcc tcttatatcc atcttgacaa gctctcctcg caattcccaa
     2941 agctcaatat aagctttcct gatttcattt cttatctcgt taggtgttag acgaaatact
     3001 tcttttccgt actcatctat tttatcccct ctttggagat atgaatactg cattaagaaa
     3061 tttctgaata tctcagcttt aaggatcaaa aagcgtggat attggggtag ttggcgggga
     3121 acttctgtag aaaagagttt ctgtgccgct tctccgtagc gaaaaagatg tgccagggat
     3181 tcgcagattc taaagaacgc cttttcacaa gtctggctat cggaggtttc tttataaacg
     3241 gactcgtaaa tcttcagggc ttcctgaaaa gtccctttgt caaacaattc atcagcttct
     3301 tttagcttag aagctgcacc aactccctgg agaaaaaata ctaaggacaa tcctaaaatg
     3361 atagatttcc aaatcatttt gatgccctcc ttcttttgct ccttttgact tcaaggttga
     3421 tttttgttac tcaaccctaa tacaaaaaag agaaacaatt attccctaaa aaaaggaact
     3481 cctcagcaga gaaaggcatg atctgttcct taatcttcta atcggacgga aatacccaca
     3541 tttacactaa atcgaccaaa aaagtcaaag tagatacgtt aagaccttga gagattagcg
     3601 gtctcgcttt gccggagaat atagaacttc tctaacataa tgacattcat ttggtaatat
     3661 tcagcataaa aactcttctt tgctttccct cgttaattct gatattagta acaatcatcg
     3721 gagggctagt cctaaccggt cagacagtgc atataaaatg cagcttgaac ctttttccta
     3781 tctttttgtt acattttttt ctaacaactc tctaaagacc aacttcatcc aacttgaagt
     3841 aacttgcttc cactcgaagc aacttgatag ggcttaactc ttgacaagac aagggatttt
     3901 ggctaatctt gccaaagatt aatgagttag atggaaagac acggagattg gttctatttt
     3961 gggttctccc gcaagaaaac ttaagggatt tcgcctggct tcgcacgctc aagactccca
     4021 gtctcggaca ctatttttta acagatttct aacaggggtc attgtgacga ggcgggcatc
     4081 catataccaa acctagtacc ccacctaaag cgtaagcgac aggtgggttt attgttccca
     4141 ctcgttggta tgaaccacaa tggttcatac cgttgggtgg ggtacagtct aaagtgggat
     4201 atataggatc agattgattc gaagccggca aacccacatt gccctcaatg tgggtttaat
     4261 gcccaccttg gggcaaggtg ggaatactaa tagcttgatg tgatatatat agaatgacgg
     4321 ggcatctatc cattgtcatt gcgagtcccc gtaggggcga agcaatcttt tgttgaaagc
     4381 gagattgctt cgctcccccg atgaatcggg gtcgctcgcc ctgaaccaga acggtttagg
     4441 gctcgcaatg gcatttcact aagcagatgt attagttaaa tccccttgct ttctttttcg
     4501 atccagtttt tcagagtagc cataaccgga gttttgcccg aggaataaac tttcccattt
     4561 atcaccagcc cgggagtcat cattattccg tatgaagcaa acttcgatat atcggtaatc
     4621 ttctcgacat tggcttcaat ctttagctca gtcagggcat cgcgcacatt ttgttctaac
     4681 tttttgcaat tagaacaccc tgggcccaaa atttttatat ccatttaaca tccctcctta
     4741 tagtttaaag attaatccat agtatataaa ttcctgcgaa aattatcagc agtccactta
     4801 cttttttagc caagtttgag aaattcgtta ttccgcggga ttgagcaaaa ctctcgacaa
     4861 atccagctga tacacctgct gcaattatca aagcacagtg tcctaaggcg taaacaaaaa
     4921 gaagcgagac cccatacatt atccgagcct ttgtggcgac aaaagctaag ataaccgcta
     4981 aaatcggtgt agcacaggga gaggagacca tcccaaagag aaggcccaaa aggaaagccc
     5041 ctaaaacccc tttgtgttta gtttttaaat ccactgtctt aggaaaggaa atctttatcg
     5101 cccccacaag atataagccc ataattagag ccacacccgc tgctacgaaa taccacaact
     5161 tccctacatc cccgaacagt cttcctaaga gggaggctac aactcccaat gcggtaaagg
     5221 tgatagatag gcctaaggcg aaagttaaag aataaagcaa tgctttcttt ttatctccct
     5281 tagaataacc tcccacaaat ccaatcacca atggaataat tgccagaacg caagggctgg
     5341 aggctgatag aattccaccc aagaagatca agatataagc caagaatggg gcattattta
     5401 tgacccctga tatatttccc agaagagatt ccacttattt gaccccgatt tcaagcagtt
     5461 tttgttgaat ggaaagtttg tccataaatc cctcgtgtcg gtaaacctca ttgcccgccg
     5521 agtcgaaaaa aatctgcgtg ggaattagtc ttatattata gaaattggct tgctctggtt
     5581 catagcgaat atccactatt ctgatagttg ctcttccttt gtactcttcg cttaattctt
     5641 ccaagatagg ctgcatcatc ttacaggcct tacaagttcc cctgcctaaa tcaatcagag
     5701 cgggtagatt tttatttaag gtatccgaac tattttcaga cagaacagcg ctttcagaga
     5761 gcgaagctgg tttatttccg agaccaatct catttttttg tttcaagatg aacaaaccta
     5821 caattagaat agtaatagct aaaataacca gaagaatggt gcgatttttc ataaccttct
     5881 cgaattaggt ttagttttta gttcttataa agttattttg cagtgttttt aactttctta
     5941 ttctctttat cggcaataaa agtttcgagc tgctcttgga agttagacaa gaatttgtct
     6001 ttttcctcac aaaattgcca taccgattcg atttctttaa aagtattatc ctttccagag
     6061 aatttttgga aacccaaggc agaccaaaat atcttatacc tcttagcaat cagagagtct
     6121 ttatcaatat tgtattcctt aaagataata tgtccatctt catatggttt agtatagaat
     6181 tttattaggc cttctttagc aaaactacca gcatttatgc aacaggaaca ctgaaccgtg
     6241 ggatgaaaat gaattactaa taaactgtca aaattaaaac tgagggtaga gtccacaacc
     6301 tttttgcttg catcattgac attctttttg aggggagttt gtcgattttc attttggcta
     6361 tggccactcg tatacatcat caaaaagacc aagacataaa cgagacaaaa tactggactt
     6421 ttcttattca ttttctaaaa taaagctccg gatgccattc cgaccatagt tgacattacc
     6481 accgctaaaa taatgtaagt cactgctttt tttctcattt tacaaataca ccatagatta
     6541 aaccagaaag agtgcaaaac gctgttatca gaatcatata aactaaagtt cgtttacttc
     6601 ccattatttt tctaacaact aagatactgg gtaatgaaat cacaggatca gccagaagat
     6661 aagccagcaa tggtccttta gctaccccca aacccagaaa cattttggcc attggcacct
     6721 ccactagggt cggaaagtac ataaagaggc caaacaagac tgctaataaa ttactgccta
     6781 gagtattagt tcctacgtag cgtcctaaat aattcctggg aaatattgcc cctaacattc
     6841 ctgctacaaa tattccgatc agaagtaaag ggaagatcat tttaaagaaa ttataggttt
     6901 ctgttaacca gcttaatatt tcctcttttt ggaaccgaaa cttcagttgc aacagtagaa
     6961 ctattattag gcccaaaagc actggaattt ttatccctcc ttctatcttc gaagctccaa
     7021 aaataagaat agcaaaaagt gtgacaaaaa atactaaaag tttctttcgg acaagttcat
     7081 caattttctg gcgcaatgac atctttttaa ttccctctaa attcttttgt tcttctaata
     7141 aactctcaac cggttcgcag ttctcttctt cgtgcggaaa taacccggaa attataatcc
     7201 ctgtaagaac agcaaaaccg attgaaagga ctattcttga aaaagcaaaa tcccaaccca
     7261 gaacacttcc agtaaaaaga atcgctaaga tgttggtagc tggagcggta tataaaaagg
     7321 cgattgccgg gccaatccca gcccccttct tttttatgcc agcaaataaa ggcaatactg
     7381 tgcaggaaca aactgccagg agtaaaccag ccatcacaga aaaaagataa gccttgggcc
     7441 ttggcgcttc tggccctaag tgttttgtta tatgttcctt gggaagtaga gccgatattg
     7501 ccccggctat aaagaaagct ggaacaagac acgtcaaaac atgagccgaa agatattcta
     7561 aaagggcatt ccaaccactt agaagtatct gggagaaagt cataaacttt tcctcttatt
     7621 ttgatttttc caaagaaaaa tatctctttt gaaaccaaag agccaagctg accagactga
     7681 ttagcaccgg gacctctacc aatggtccta taactgcggc aaaagcgacg ccggatttga
     7741 tgccaaatac ggctactgct accgctatag ccaattcaaa gttattgctg gcagcagtga
     7801 aagcgatagt ggcagctttg gcataattcg ccccgatttt tttacccatc cagaaactca
     7861 gcaggaacat aactacaaaa taaatgcata aaggtatggc aattctgacc acatccaaag
     7921 gtatcttaac aatgtactca cccttgagac taaacatcac cacaatggtg aaaagcaaag
     7981 ctatgagggt gataggacta atctttggga tgaacttgct atgataccat tctttacttt
     8041 tgagcttaat aaggacgaat cgggttatga ttcctgcgat aaagggaatt cccagataaa
     8101 taaatacgct ttctgcaatc tgcctcatac tgacatccac ggcacttcct ttcaagccga
     8161 accaaaccgg cagcacggtt ataaaaacat aagcatacag actatagaac aacacctgaa
     8221 aaatgctgtt gaaggcaacc agcccggcag cgtattcggt gtcaccctta gccagctcat
     8281 tccagacaat caccatagct atacatcgtg ccaagccaat cagaattaat cccaccatat
     8341 attcgggata gccgcgtaag aagacaatcg ctaagataaa cattaagact ggtccgataa
     8401 tccagttttg gaccaagctt atccctagca ccttccaatt gcgaaacaca tcccccagct
     8461 cttcatattt gaccttggcc aggggcggat acatcatcaa gatcagcccg atagcaatgg
     8521 ggatattcgt ggtgccaacc tgaaagcgat taataaaaga atcaatcccc ggtatgaaat
     8581 atcccgctcc cactcccagg cacatagcca gaaagatcca aagagtcaac aacctatcga
     8641 aacatgaaag tttcttccct atatcagcca tggcaaagtg cgccttttct tgatttgctc
     8701 tgtttgcact tatatccata aacctgagag agttttctcc tcagtctttc ttggtctttc
     8761 ttaattaggt gattatccga aagggcgttt tcgataacct gaaaaatcgc cctttcaaaa
     8821 tcgtttttgg gtttaaaaag agaatagaag atccataatc cctctctgct atcttctgcc
     8881 agatcaactg aacgcaagac cgtaagatgc ctggagactt tggactgagg gaggtccaag
     8941 gccgcttcca attcacaaac gcaaaacttc ccttttagga gaagcttcag gatgcgaagc
     9001 cgagtctcat cacccagggc ttttaacact ttagcaggtt gttgcataaa atctcccttt
     9061 tgatattcgt atatccgaat atacgcatat aatatagctt gtgtttgtgc agtgtcaatc
     9121 acaatgtttc gataaataac agattggcgg agccgatgtg gtcctaatat gggtacgata
     9181 acctcgaaga ggtgctaagg aagatatgga actatctata atttgagaat ctcccctgac
     9241 ccctcttttt caaagagggg aatgaagggc tccccctttg gaaaaggggg ataagagggg
     9301 atttaaagaa aggtcagtgg gtttctgcca ctcttgtaag cgggatttcg caaagcttaa
     9361 ttttcgcagg aatgacgaat aaaaaagtag tggcaattca tgaattgccc cttgtagagt
     9421 cttaaatcaa aacggataaa atctcgcctc agtccagaaa tcttcctgca gaaccctctg
     9481 ttgattcacc agggctttca gatgttcgcc ttcccactca gccaaaaagt taaacagggc
     9541 tttggcatcc aaatccccaa tcttctcgct ggagttcttg taaaaatcaa tggaattctg
     9601 ctccagcata attccaatac ttaaagctga catttcaaag ctttgttctt tcaataattt
     9661 tcccctgaaa ttttctgaaa agatgggata agacgagact gtatccaatt tagaaccctt
     9721 atcttttttc tcgaatttaa attttccatt ttccttgaac ttcttatact gctcccttaa
     9781 cattttctca tgtttcaatt catcctcagc caagagctta aatacgtctt ttgccttagg
     9841 gtccttggtc ttttcagagg ccatcttata aagttgaaaa ccatcaattt cagttttaat
     9901 ccctcttttt aaaatttcca aaacatcctc tttttgcata aacctcccct atttcaattt
     9961 ttctaattgt tctatcaact cctgattatc tctggcctgg gggatatcgt aaaccttaac
    10021 atactgcaca atcccctgct tgtcaattat aaccgtggct cgattagtta tccccaaatc
    10081 ctttaagtaa agcccatacc tggaggcaac ttcccctttg cgatgaaagt cggatagtaa
    10141 cggataggtc agtttcagtt tttccgcaaa cgccttgtga gaccaaacac tatccacact
    10201 aattccaaaa atcacagcat tcaagctttc aaacctcttc agatcgttaa taaaacaggc
    10261 atgctcctga ctgcataccg ggctgaaatc cgcaggataa aatgccaaga agacattttt
    10321 tcctttgtaa tcgcttaatt tatattcctt tccatctccc cctttaagca caaaatccgg
    10381 tgccttctct ccgactttga taggcataaa gcctccttgg ttaaaatcga tttacgaagg
    10441 acatgtcaga ataccctcgt atggggataa atggcatttc cataagttcc ttctcccttg
    10501 ggggagaggg ttagagccta ccctaaagcg aagcgaagag gtgaggggga taacgaagat
    10561 gattctgacc ctctctccgc ctttggcggg attttcaact tagtcctccc cctcaaaagg
    10621 gaggagatat tttggacgcg cccttagggg aaagttcgtt atatgtgata cgcttgtggc
    10681 agaggatttc tttgatgggc gagaaaacgt aggcgaatat cagttagctt ctgttctgtc
    10741 aggaccttag tcctgacgca actgtagaaa cagagagtct ctttgataag tggaagaaaa
    10801 aacgtaggcg acccagatgg gtcgcctgca cagaatcagc tcgtctcggg acgaagtcac
    10861 ggacaagaaa tgaagcttta cctattcacc accgcatcca caattttctt gtccacattg
    10921 aatttattaa gaaatgcttc gcgctccttt ttcatattct ccacccaggc tttatattcc
    10981 tccattctgc cgtttttctg gaagaaaact ataggattta gaatctcctt gggcactttc
    11041 tttagaagct cggcattttc tttggcatcc acatccacaa tctcgtatcc aaaatccggg
    11101 tccttcttcc attttatgtt tcccgaaagc atagcctgaa gcattgctga agagtgagga
    11161 attttaacct tcagagcttt gccacctttt tcgtctctgg attctccgcc tatgtatccg
    11221 gtgttcatca tccaggtctc taaacccgga agcttagcgg caagttcact gaatcttttg
    11281 gcttgcagtc cgtgtgggct ggtgaagaat ggttgagtga aaggagagcg ggtctttcct
    11341 ctctctttgc ctgcggcaga ggttttggtg gtttcgccta acatgaaata cccggcagcc
    11401 aactggggag tggaaaagcg gacaattcct ggggtagctg catctttacc ttcatcccgg
    11461 tttagaattc caagtgagcg gagcggagga atattatcaa agctggcagc atctttgatc
    11521 gccttcatcg gaattataga cctgccattc tgactccaca ccgtaaaatc ccaaccatct
    11581 ttaggaatat catattcgtc caccacctcg tctatcttaa ctttgccact gatgaaatcg
    11641 tccacattct tagggtcagc tccagattga acgagcatat ctcttagtcg acccacttct
    11701 tgcggagtca agcgtgcctt actgaagtcg tatgttccat ctacattggg aaagacgttc
    11761 tccacccagg catctttatg aatggtgcca gcataaagca ccggctccga ctccacagta
    11821 agcccgtagg ttttggcgaa acagccattc tccgtgatgg tgagcgtgcc atccggctgc
    11881 atcacaatca tatcatcttg aatgggctga gccagctccc cttgcttact gaaagtggtg
    11941 gtggtttttc ctgtgccgga aagccccagg attcccatag tcatagactt gccattgata
    12001 gtaacttttt tagcgccagc gtgaagaacc agaccgccta atctgtagac gtattcattc
    12061 aacatacgca ggacgccctt cttggtctca ccgaaataat ccgagcccat aacataagtg
    12121 gtccaattgt tcaaatccac tacaatcgcc aattttcccg gcatatcctc gagaaccaat
    12181 ccagcggtat aaaccagtcg gaacttcggc tcaaatggct ccttgagctg attcttgttc
    12241 tccacggcag aacgcgagaa agccaacacc tgctgcattc ccgctatgtt agccgcctcc
    12301 ggagtgtaaa accactgaac cggcacagct ccctttccgt atccataata ggcgttgatc
    12361 tcgatcagtg tgcccttttt ttcgatgtaa gccctctgtt tttctataag ctgttgggct
    12421 ttggctgggt caattatgct gccggagaag ttgctctttt cgctttccgg tgcgatgcaa
    12481 taggtgaatt ttgccattcg ggctttgtat cgagaaaact tatcgatgtt gccatattta
    12541 gtcttcatta tggcaggggt atttttcaga gccaactccc gcagttgctc ttgagtcgga
    12601 tcaacgatat atttcacatt gtagggcttg ggttttaagc tcattttacc tcctgaaatg
    12661 tttttagctg gttttcagat tgctactaat ttcacaaatt caaggtattt tgtcaagtgt
    12721 tttccgcagg aagaattggt tgacaaaggg gcagtaggaa actatactta gtctgttatg
    12781 aaatccatag ccgattttca cgttcagtga gtaacccaca gctctgctgt ggggcaccct
    12841 tggccgatgg cgtaacggta cctcggatgt tatgcgggtc aatataaaca atgttaggcc
    12901 aagaagagga tgactatgcc aatgaaagag tggttcgcag aagcagaggg acacaagatt
    12961 cgcgtcgaga acacgtggac cggcggcaca cgcttgtaca ttgatggaga gtgccgcgat
    13021 cgaaacacag ggtggttcgc agcttcgaaa acacgctggt tgtctgctcc gctaaagccc
    13081 ggagacccta aaagcgcaac agtggaggtc tttgttaaag cactgttcac tgttaaggcg
    13141 caaatctgtg tagcaggcaa gcc
//
LOCUS       DASWLN010000043         6805 bp    DNA     linear   ENV 20-FEB-2024
DEFINITION  MAG TPA_asm: Candidatus Zixiibacteriota bacterium isolate
            SMAG_U2949 k141_934836, whole genome shotgun sequence.
ACCESSION   DASWLN010000043 DASWLN010000000
VERSION     DASWLN010000043.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37960594
            Sequence Read Archive: SRR10376091
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Candidatus Zixiibacteriota bacterium (soil metagenome)
  ORGANISM  Candidatus Zixiibacteriota bacterium
            Bacteria.
REFERENCE   1  (bases 1 to 6805)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 6805)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (26-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/12/2024 08:28:26
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 1,440
            CDSs (total)                      :: 1,416
            Genes (coding)                    :: 1,412
            CDSs (with protein)               :: 1,412
            Genes (RNA)                       :: 24
            rRNAs                             :: 1 (16S)
            partial rRNAs                     :: 1 (16S)
            tRNAs                             :: 21
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 4
            CDSs (without protein)            :: 4
            Pseudo Genes (ambiguous residues) :: 0 of 4
            Pseudo Genes (frameshifted)       :: 0 of 4
            Pseudo Genes (incomplete)         :: 3 of 4
            Pseudo Genes (internal stop)      :: 1 of 4
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..6805
                     /organism="Candidatus Zixiibacteriota bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_934836"
                     /isolate="SMAG_U2949"
                     /isolation_source="soil"
                     /db_xref="taxon:2053527"
                     /environmental_sample
                     /country="USA: Oregon"
                     /lat_lon="42.99 N 123.63 W"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            complement(157..894)
                     /locus_tag="VGB16_01745"
     CDS             complement(157..894)
                     /locus_tag="VGB16_01745"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011687882.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016779 - nucleotidyltransferase activity
                     [Evidence IEA];
                     GO_process: GO:0009058 - biosynthetic process [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="sugar phosphate nucleotidyltransferase"
                     /protein_id="HEX9916436.1"
                     /translation="MKGVILAGGLGKRLYPLTKITNKHLLPIYDKPMIFYPLQTLINA
                     GIKDILIVSGGNHAGEFLRLLGDGKEFGLKHINYTYQEGEGGIADALRLAEHFADNEK
                     ICVILGDNIIEGNIISAVKDFEKQPEGAKVLIKEVSDPQRFGVPEIAGGKIVSIQEKP
                     EKPKSKYAVIGIYMYNAQVFEIIKELKPSARGELEITDVSNAYIKQGTLTYEELKGWW
                     TDAGTYQSLLLASNLVAKGGANKLSLK"
     gene            complement(1397..2182)
                     /locus_tag="VGB16_01750"
     CDS             complement(1397..2182)
                     /locus_tag="VGB16_01750"
                     /inference="COORDINATES: protein motif:HMM:NF004139.0"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="50S ribosomal protein L25/general stress protein
                     Ctc"
                     /protein_id="HEX9916437.1"
                     /translation="MKEVNLEVKKRESRGKEKAKKLRKSEIIPAVVYGAGEEALPLEV
                     KLKDLKAFFKATKGENVIINLNIDDGKLESRKVLLREVQKDPVWDNILHVDFQHISLT
                     EKITVKIPIHLIGLSTGVKNQGGILEHVLRELEIKCLPTEVPQHIDVDVTELEIGDVI
                     HVKDLKVEKLEILTDPDRSIVTVVPPTVFKEPEVAAVVEEGKEPELVGAEKEGEEAAE
                     GEEGKEEKEGEAKAETKEEKKEGRREEKKEKKEKKEEKKEEKK"
     gene            complement(2205..3146)
                     /locus_tag="VGB16_01755"
     CDS             complement(2205..3146)
                     /locus_tag="VGB16_01755"
                     /EC_number="2.7.6.1"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_018128240.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0005737 - cytoplasm [Evidence IEA];
                     GO_function: GO:0000287 - magnesium ion binding [Evidence
                     IEA];
                     GO_function: GO:0004749 - ribose phosphate diphosphokinase
                     activity [Evidence IEA];
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_function: GO:0016301 - kinase activity [Evidence IEA];
                     GO_process: GO:0006015 - 5-phosphoribose 1-diphosphate
                     biosynthetic process [Evidence IEA];
                     GO_process: GO:0009156 - ribonucleoside monophosphate
                     biosynthetic process [Evidence IEA];
                     GO_process: GO:0009165 - nucleotide biosynthetic process
                     [Evidence IEA];
                     GO_process: GO:0016310 - phosphorylation [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="ribose-phosphate pyrophosphokinase"
                     /protein_id="HEX9916438.1"
                     /translation="MDKKVKLITGNSNKPLAQKISKHLGVPLAECEVSRFSDGEIFVK
                     ISENIRGMDVFIIQSTNPPAENIMELLVLLDAARRASAKRITAVIPYFGYARQDRKDQ
                     PRVAISAKLMANLVDEAGADRVLTVDLHSPQIQGFFDIPSDHLFSSSIFNDYFLKLRL
                     SNLVTVSPDVGSVKMCRAFAKVLGTPLAIVDKRRPSPNHSEIMNIIGEVEGKNVVIRD
                     DMIDTGGTLAEAAEALARKGAKDIYACCTHPVLSGNAIKKIEKSSIKKVIVADTISTE
                     NKKLTKKFEIVSMAGLLAEAIKRIHLEESLSVLFEGA"
     gene            complement(3277..3348)
                     /locus_tag="VGB16_01760"
     tRNA            complement(3277..3348)
                     /locus_tag="VGB16_01760"
                     /product="tRNA-Gln"
                     /inference="COORDINATES: profile:tRNAscan-SE:2.0.12"
                     /note="Derived by automated computational analysis using
                     gene prediction method: tRNAscan-SE."
                     /anticodon=(pos:complement(3314..3316),aa:Gln,seq:ttg)
     gene            complement(3549..3860)
                     /gene="spoVG"
                     /locus_tag="VGB16_01765"
     CDS             complement(3549..3860)
                     /gene="spoVG"
                     /locus_tag="VGB16_01765"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011419182.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_process: GO:0030435 - sporulation resulting in
                     formation of a cellular spore [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="septation regulator SpoVG"
                     /protein_id="HEX9916439.1"
                     /translation="MEITEVRITLRDEEKLKAFANVTFDNAFVIRGLKVINGNEGYFV
                     SMPSRKRKDGTHQDIAHPINNEMRKTIEDKVLAAYEEELKKKGTGTETGTGTVTGTDT
                     T"
     gene            complement(3960..5009)
                     /gene="ispE"
                     /locus_tag="VGB16_01770"
     CDS             complement(3960..5009)
                     /gene="ispE"
                     /locus_tag="VGB16_01770"
                     /EC_number="2.7.1.148"
                     /inference="COORDINATES: protein motif:HMM:TIGR00154.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0050515 - 4-(cytidine
                     5'-diphospho)-2-C-methyl-D-erythritol kinase activity
                     [Evidence IEA];
                     GO_process: GO:0016114 - terpenoid biosynthetic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="4-(cytidine
                     5'-diphospho)-2-C-methyl-D-erythritol kinase"
                     /protein_id="HEX9916440.1"
                     /translation="MEKISLLSFAKINLCLYVLSKRKDGYHQILSVIQAIDLADKLEL
                     TKTASGIKIECDSPDLPSDKGNLAYQAASAFFKKSKTKGGVRLKLYKKIPLASGLGGG
                     SSNAAFVLKGLNQLYETDLSKKELSSLGAKIGSDVPFFFSEGQALVKGRGEVVQNIDI
                     FDKYWLVLVKPNFEVSTARTYKALKRVSEKPYRGGRVSRCRIRTRCPTYPTDPGDEES
                     ITYLKTHNSCNHVGRAPSPAKQAFHKSLKIGLTTKPREVNLKFCRGKFSFFRAISSWE
                     NHLEMVVLKEYPIIKNIKETLINLGAIKASMSGSGPTVYGLFETESQAEEVKDKIGKG
                     DWQIYLTRPTRLMKI"
     gene            complement(4997..6523)
                     /locus_tag="VGB16_01775"
     CDS             complement(4997..6523)
                     /locus_tag="VGB16_01775"
                     /inference="COORDINATES: protein motif:HMM:NF025418.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="capsule assembly Wzi family protein"
                     /protein_id="HEX9916441.1"
                     /translation="MRREVLLFFFLLLVWSSETCYPTCLENISLDNWVYRDLEELHTA
                     GLLPGLHKNIRPYSTGDIVSSLKDIRQRMNTGELKLDEDQVWLIQKLEEKFKYELEES
                     CSQEGKIKYGIDPLFYLYQEKDSTTFRFKMRIEGAIQLEDKFLLKGRAAVDNQSDRET
                     DYYVRKWKKNLIGTMDEAYTIIDFRYLKLFFGREALAWGPSFRDNLLLSNHFPPLDML
                     KLESEIGSFKLLYFTTILDQKEISDGNWAKRYFSAHRLDWKSNFGLEIGLSEVILYGG
                     SNRNIETYYLNPLLPYYAEQFNHDTDDNPLWSIDFNLVFKSKELYGEFLIDDFQYDFK
                     TEPQQIGFQLGINWHEPFKFKKTFLNLEYTRINKWVYGEYPSWNIYTYHNVGMGSFLG
                     PDADDISFKILHHLKGDFSFSISLEYKRKGEGRIDEAQEPAVPFPENFPSGIVEYTKG
                     VNITSFYRPNSNLGINFWFDYQNVKNYLNLGNNHKNFYEVGIELNWTFLKENLFNWKK
                     "
BASE COUNT         1758 a         1637 c         1250 g         2160 t
ORIGIN      
        1 agtgggttac aggggttagg ttctcttctg ctaccggtgg gctcgatcct tcaaccattc
       61 gataaactca tggtgctgag cgaagtcgaa gcacaagctc aggatggtga gctagtcgaa
      121 ccataaatcg agcccctaca atatcaacaa atcaatttac tttaacgaaa gtttgttcgc
      181 tccacccttt gctaccagat tacttgccaa gagtaaagac tgataagtgc ccgcatcagt
      241 ccaccaacct ttgagttcct cgtaagtcaa tgtcccttgt tttatatagg cattactcac
      301 atccgttatc tccagctccc cgcgggcaga tggttttaat tctttgatta tttcaaacac
      361 ttgcgcatta tacatataaa tcccgattac ggcatatttc gattttggct tttcgggttt
      421 ttcttgaatg cttacaattt tgccgcctgc tatctccggc acgccgaacc tctgcgggtc
      481 agaaacctct tttatcaata ctttagcgcc ttcgggttgt ttttcaaaat ccttaactgc
      541 ggagatgatg ttcccctcaa ttatgttatc tcccaggatg acgcatatct tttcgttgtc
      601 cgcaaaatgc tctgccaatc tcaaggcatc cgcaattcca ccctcccctt cctgataagt
      661 gtagtttata tgttttaacc caaactcttt tccatcgccc agaagtctta agaactcgcc
      721 agcatggttt cctccggaca caataagtat gtcttttatg ccggcattta taagagtctg
      781 caagggataa aatatcatcg gcttgtcata tattggaagg agatgcttgt tagtaatttt
      841 ggttaaagga tataatcttt tccccaaccc accggctaaa atgacacctt tcataaaatc
      901 ccctttttat ttatttatct ttcgcgtagg ggtggagttt acctccaccc gcatattttc
      961 tttaatgtag ggacaggttc cacgctttta gcgggatgtt ccacgcgacc tgtccctacc
     1021 aaaaatctat ttcctcgtag ggacggaaat tcatggttcg gctttgctca ccatcctgag
     1081 cttgtgcttc ggcttcgctc agcaccatga gtttatggtt cggctttgct caccatcctg
     1141 agcttgtcga aggatcccgt ccctcgtagg cgacattccg gaatgtcgcc tacaaaatct
     1201 ctacattcct ctcccttgaa gggagaggat aaagttgaaa atcccgccaa aggcggcgag
     1261 tgagggtgaa tatcgtttat caaaaaagcc gagaacagac ctccctaaaa tgaaaactta
     1321 aaaattcgtt acgaattttt aagttttcat ttccatcctc aatttcttat ttagtataaa
     1381 aatcgctaaa aagcaactat tttttctctt ccttcttctc ctctttcttc tctttcttct
     1441 cttttttctc ttctcttcgt ccctctttct tttcctcttt agtttctgct ttggcttccc
     1501 cttctttttc ctctttgccc tcttctcctt cagctgcttc ctcaccctct ttttcagcac
     1561 caaccaattc cggctctttg ccctcctcca ccacagctgc cacttccggc tctttaaata
     1621 cagtcggagg aacaaccgtt acaatcgacc tgtccgggtc agtcagtatc tcaagttttt
     1681 ccacttttag gtctttgaca tgaatgacat ctccgatctc cagctcggtt acatctacat
     1741 caatatgctg tgggacttcg gtgggcaggc attttatctc caattccctt aggacatgct
     1801 ctaagattcc accctgattt tttactccag tggagagacc aatcaaatga attggaatct
     1861 tgaccgtgat tttctcggtc agggagatgt gctgaaaatc aacatgtaag atattatccc
     1921 aaaccgggtc tttttgaacc tcgcgcagaa gaacctttct gctctccagt ttgccatcat
     1981 cgatatttaa attaataatc acattctcgc ccttagtggc tttaaagaac gcttttagat
     2041 ccttcaactt gacttctaaa ggtaacgctt cctcaccagc tccatacacc actgccggga
     2101 tgatttcact ctttcttaat ttcttagctt tttccttacc tctggactct ctttttttga
     2161 cttctaagtt gacctctttc aatttatcct cacctctcta ttctttaagc tccttcaaac
     2221 aggacgctca gcgattcctc caaatgtatt ctcttgattg cctcagccaa aagaccagcc
     2281 atcgagacta tctcgaattt tttggttaac tttttgtttt cagttgaaat ggtatccgcg
     2341 acgatgacct tctttatgga agatttctct atcttcttaa ttgcatttcc ggacagaacc
     2401 ggatgggtgc agcaggcgta aatatctttt gctccttttc gggctaaggc ttcagctgct
     2461 tcagctaaag ttccgccggt atctatcata tcatccctta tcaccacatt ttttccttcg
     2521 acctcgccga ttatattcat aatctctgaa tggttgggag atggccttct tttatccact
     2581 atggctaagg gtgtgcccaa aaccttagca aaggcacggc acatcttcac gcttcccaca
     2641 tccggagaca cggtaaccaa gtttgaaagc ctcagtttca agaaataatc attgaaaatt
     2701 gaagaggaga aaagatggtc tgagggtata tcaaaaaatc cttgaatctg cggggaatgt
     2761 aaatccacag tcaaaaccct atctgctcct gcttcatcca ccaggtttgc cataagcttg
     2821 gcagatatgg ctactctcgg ctggtctttt ctatcctgac gtgcatatcc aaaatagggg
     2881 atgaccgcag ttattctctt ggctgaagcc cttctggctg catccaagag aaccaaaagt
     2941 tccattatat tttctgccgg cgggttagtg gactggatga taaaaacatc catcccccga
     3001 atattctcgg agatcttaac gaatatctct ccatctgaga agcgcgaaac ttcgcattca
     3061 gccagaggaa ctcccagatg cttacttatc ttttgagcta atggcttatt cgaattgccg
     3121 gtaatgagtt tgactttttt gtccataagg aatatttaaa gttacctaag gttattgaat
     3181 ttcttgattt aggattgggt aattttttaa attcaaaact ccaaactttt aaactgcttt
     3241 tatggggtgg cctgtgaact gttaaccgtg aacctactgg ggcgccagga ttcgaacctg
     3301 ggaatgcaag atccaaagtc ttgtgactta ccgcttgtcg acgccccaaa aaatttataa
     3361 tgaaaggagt gaagcctccc cgtgataaat cacagagctt cctatatata aaaggctctc
     3421 ggcttccctc ggcttccctc ggcttccctc ggcttcgccg agaggcgagc gccgagaggc
     3481 gagcgccaaa ggcgaccgaa gctctcctcg ccatttccgc caaaggcttt ctggcagagg
     3541 cgagtaattc aggtcgtgtc agttccagtt acagttccag ttccagtttc agttccagtt
     3601 cccttctttt tcaattcctc ctcgtatgcc gctaaaactt tgtcctcaat cgtcttgcgc
     3661 atctcattgt taatcggatg ggctatgtcc tgatgagttc catcttttct tttgcggctg
     3721 ggcattgata caaagtatcc ctcgttgcca ttgataactt taagcccacg aatcacaaag
     3781 gcgttatcaa aagtaacatt ggcaaatgcc ttcagtttct cttcgtccct taaggttatc
     3841 ctcacttcgg tgatctccac tttttcctcc tagcaagaaa ttaaaaacta gagttgttta
     3901 actaagatga tggaccaaaa gatctataaa tgaagatttt aaaaattcgt tacgaatttt
     3961 taaatcttca ttaagcgagt tggtcgtgtc aaataaattt gccaatcgcc cttacctatt
     4021 ttgtctttca cctcctcagc ttgagattcg gtctcgaaca gtccatagac ggtgggtcca
     4081 ctccccgaca tggacgcttt aattgctcct aagttgatta aggtttcctt tatattctta
     4141 attatcgggt attcttttaa gaccaccatc tccaaatggt tttcccagga ggaaatggca
     4201 cgaaaaaaag agaatttccc cctgcaaaat tttaaattaa cctctctggg cttagttgtc
     4261 aagccaattt ttagggattt atgaaaagct tgtttcgcag ggctgggagc cctgcctacg
     4321 tggttgcacg aattatgtgt cttgagataa gttattgatt cttcgtcgcc aggatccgta
     4381 ggataggtcg ggcaccttgt gcgaattctg catcttgaga cccgaccacc gcgatacggt
     4441 ttttcagaga ccctttttag ggctttgtaa gtccgagcag ttgatacttc aaaattgggt
     4501 ttaaccaaaa ccagccaata tttgtcgaaa atgtcgatat tctgaacaac ttcccctctc
     4561 ccttttacta aagcctggcc ttctgaaaag aaaaagggga cgtctgaccc gatcttagct
     4621 cctaatgaac ttaactcctt tttggataaa tctgtctcgt aaagctggtt tagccctttt
     4681 agaacaaaag cggcattact gcttccacca cccaagccac tggcaagcgg aatctttttg
     4741 tataacttga gtcttactcc cccttttgtt ttactcttct taaaaaaagc tgaagctgcc
     4801 tggtaagcca gatttccctt atcagaagga agatcagggc tatcgcattc tatctttatg
     4861 ccagaagcag tcttagtcag ttcaagtttg tccgccagat ctatggcttg tatgactgag
     4921 agtatctggt gatacccatc ttttctttta ctaagaacat agaggcacag gtttattttg
     4981 gcaaaagaaa gcaaacttat tttttccaat taaacaaatt ctcctttaag aaagtccaat
     5041 tcagctctat tcccacttca tagaagtttt tgtggttgtt acccaaattg agataattct
     5101 ttacattttg atagtcaaac caaaagttta tacccaagtt cgaattaggt cgataaaaag
     5161 aagttatgtt taccccttta gtgtattcaa ctatccctga gggaaaattt tcaggaaaag
     5221 gaacagctgg ctcttgtgcc tcatcaattc tcccctcacc ttttcttttg tattcaaggg
     5281 atattgaaaa agagaaatct cccttcagat gatgcaggat tttaaaggaa atatcatctg
     5341 catccggtcc caaaaagctc cccataccca cgttgtgata agtataaata ttccaagaag
     5401 gatattcccc ataaacccac ttgtttattc tggtatactc taggtttaag aaggtctttt
     5461 taaatttgaa cggctcatgc caattaattc ctaactgaaa accaatctgc tgcggctcgg
     5521 tcttaaaatc gtattggaaa tcgtcaatta aaaattctcc gtagagttct ttagatttaa
     5581 agacaagatt gaaatctatg ctccataagg gattgtcatc tgtatcatga ttgaactgct
     5641 cagcataata aggaagaaga gggtttagat aatatgtttc tatgttcctg ttcgacccgc
     5701 catacaagat tacttcggaa aggccgattt ccagaccaaa attggatttc caatccaatc
     5761 tgtgagctga aaaatatctt ttagcccaat taccatctga aatttccttt tggtccagga
     5821 ttgtagtaaa atagagtagt ttaaaagagc cgatttcgct ctccagcttg agcatatcca
     5881 agggaggaaa atgattggac aatagaagat tatccctgaa gctcggtccc caggcaagag
     5941 cctctctgcc gaagaagagc ttgagatatc taaaatcaat tatggtataa gcctcatcca
     6001 tagttccaat caagttcttt ttccattttc tcacgtagta gtctgtctcc ctgtcagatt
     6061 gattatctac tgctgccctt ccttttagta agaatttgtc ttctaattga attgctcctt
     6121 caattctcat tttgaaacga aaggtggtgc tgtctttttc ttgataaaga taaaaaagtg
     6181 ggtcaatccc atattttatc ttaccctctt gagagcaaga ttcttccagc tcatacttga
     6241 atttttcctc taatttctgg attagccaga cttggtcttc atccaatttc agctcgcccg
     6301 tattcatcct ttgtcgaata tctttgagag aggaaactat atccccagtg gaatagggtc
     6361 ttatattttt atgcaatccg ggcaaaagcc cagctgtgtg aagctcctct aaatccctgt
     6421 acacccaatt gtctaaggag atattctcca agcaagtcgg ataacatgtt tcactcgacc
     6481 agaccaaaag caaaaaaaag aaaagaagaa cctctcttct catattatca ttgttgtcgg
     6541 gcagtcttgc gacaacccga cctacttttc taacgaattc ttcaattgta ggggtcggat
     6601 tctctcaaac ccctcttaat ctccccttat caggggagta aaacgctttt tgtccccctg
     6661 acaaggggga tacagggggt tagattcttc cgccccaccc gcatgggctc gcttcccaac
     6721 cctcataaga ccaagattct tcactctgcg gggtttaacc tgaggcaacc cagacccttc
     6781 gcttcgctcc cttcgacttt gctca
//
LOCUS       DASWLN010000044         3600 bp    DNA     linear   ENV 20-FEB-2024
DEFINITION  MAG TPA_asm: Candidatus Zixiibacteriota bacterium isolate
            SMAG_U2949 k141_1164170, whole genome shotgun sequence.
ACCESSION   DASWLN010000044 DASWLN010000000
VERSION     DASWLN010000044.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37960594
            Sequence Read Archive: SRR10376091
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Candidatus Zixiibacteriota bacterium (soil metagenome)
  ORGANISM  Candidatus Zixiibacteriota bacterium
            Bacteria.
REFERENCE   1  (bases 1 to 3600)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 3600)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (26-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/12/2024 08:28:26
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 1,440
            CDSs (total)                      :: 1,416
            Genes (coding)                    :: 1,412
            CDSs (with protein)               :: 1,412
            Genes (RNA)                       :: 24
            rRNAs                             :: 1 (16S)
            partial rRNAs                     :: 1 (16S)
            tRNAs                             :: 21
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 4
            CDSs (without protein)            :: 4
            Pseudo Genes (ambiguous residues) :: 0 of 4
            Pseudo Genes (frameshifted)       :: 0 of 4
            Pseudo Genes (incomplete)         :: 3 of 4
            Pseudo Genes (internal stop)      :: 1 of 4
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..3600
                     /organism="Candidatus Zixiibacteriota bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_1164170"
                     /isolate="SMAG_U2949"
                     /isolation_source="soil"
                     /db_xref="taxon:2053527"
                     /environmental_sample
                     /country="USA: Oregon"
                     /lat_lon="42.99 N 123.63 W"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            <1..474
                     /locus_tag="VGB16_01780"
     CDS             <1..474
                     /locus_tag="VGB16_01780"
                     /EC_number="2.7.1.130"
                     /inference="COORDINATES: protein motif:HMM:NF014645.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_function: GO:0009029 - tetraacyldisaccharide 4'-kinase
                     activity [Evidence IEA];
                     GO_process: GO:0009245 - lipid A biosynthetic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="tetraacyldisaccharide 4'-kinase"
                     /protein_id="HEX9916442.1"
                     /translation="LVLNRAEKADNNKMELINKLKEYNSTAPIIETEYIVEKIYNLKN
                     EGKPILPSQVKDKKILGFCGIGNAQSFKQTIEDLGVNVMKFIPFPDHYTYKDKDIKEI
                     EREALDRKADFILTTEKDGVRLILLPYSKVPIYIVKMKLEIISGETSLWDLIYGE"
     gene            464..2077
                     /gene="nadB"
                     /locus_tag="VGB16_01785"
     CDS             464..2077
                     /gene="nadB"
                     /locus_tag="VGB16_01785"
                     /EC_number="1.4.3.16"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011417139.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008734 - L-aspartate oxidase activity
                     [Evidence IEA];
                     GO_process: GO:0009435 - NAD biosynthetic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="L-aspartate oxidase"
                     /protein_id="HEX9916443.1"
                     /translation="MENELIKTDFLVIGSGMAGLSYALKVSGLGSVAIITKKGECESN
                     TNYAQGGIAAVLSSNDSFQLHIEDTIKVGMELCKEEVVARVVEAGPDCIEGLIELGVH
                     FSGTKGKFDLGIEGGHSKKRVVHAKDYTGREVEDNLLKAVRSKQNITVYENHLAIDLI
                     TQHHLKGYNPKPDEKVNCWGAYVLDIKSNRIKRFLAKTTLLATGGAGRVYLHTTNPSI
                     ATGDGVAMAYRAGTRVANMEFIQFHPTSLYHSQAESFLISEAVRGEGGKLKLKSGQTF
                     MHKYHPKAELAPRDVVARAIDTELKASGDFCVYLDITHLDADFIQKRFPNIYKHCHQL
                     GLDITKEPIPVVPAAHYICGGVITDIQGKSDIQGLYACGEVACTGMHGANRLASNSLL
                     EAVVFAHFAAESSKEEFKKDFSFPEIPPWSEEGVFDQKEWVIISHNRSEIEKAMWDYV
                     GIVRSNSRLEMAKSRIDTLLKEINEFYNLNPVTYDVIELRNVSLVADLIVSSALMRKE
                     SRGLHYNLDYPQIDDTNWRKDTILQQKVW"
     gene            2077..>3600
                     /gene="guaA"
                     /locus_tag="VGB16_01790"
     CDS             2077..>3600
                     /gene="guaA"
                     /locus_tag="VGB16_01790"
                     /EC_number="6.3.5.2"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_020402870.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003922 - GMP synthase
                     (glutamine-hydrolyzing) activity [Evidence IEA];
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_function: GO:0016462 - pyrophosphatase activity
                     [Evidence IEA];
                     GO_process: GO:0006177 - GMP biosynthetic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="glutamine-hydrolyzing GMP synthase"
                     /protein_id="HEX9916444.1"
                     /translation="MKSKSQERILILDFGSQYTQLIARRIRELGVFCEIAPFNQDMSS
                     YGDKNLKGVVLSGGPASVFDKNAPFCKKGIFEMGVPVLGICYGLQLIAKIFEGKLEKA
                     KNREYGRAVLKIDYADMLLKDVKNSSVVWMSHGDNVIRVPKGYKILAHTEDIPYAAMA
                     NLKQRIFGLQFHPEVAHTQEGKKILKNFLFTICGCKGTWTPASFINSQIKEIKKKVGK
                     DKVLCGLSGGVDSSVCAVLINRAIGKQLNCIFVNNGLLREGEAGEVVETFKKLKMNLT
                     FVDAKDKFLSALKGITDPEEKRRRIGKLFIQVFEVQAKKIGKTKFLAQGTLYPDVIES
                     TSSRGPSATIKSHHNVGGLPAKMKLKLIEPLRELFKDEVREIGRKLDLPEKIIGRHPF
                     PGPGLAVRVLGEVTEGRLSILRKADRIFIQNLKESGFYNRVWQAFCVLLPIQTVGVMG
                     DERTYENVVALRAVTSLDGMTADWARLPYHLVEKISNQIINQVKGVNRVAYDVSSKPP
                     "
BASE COUNT         1123 a          637 c          905 g          935 t
ORIGIN      
        1 ttggttttga atagggctga gaaggcagac aataataaga tggagctgat taacaaacta
       61 aaggaatata actctactgc tccaattata gaaactgaat acatagtgga gaaaatatat
      121 aatttgaaaa atgagggaaa acctatcttg cccagccagg ttaaagataa gaaaattttg
      181 ggtttttgtg gaatcggcaa tgcccaaagc tttaagcaaa ccatcgagga cttgggtgtt
      241 aatgttatga agtttattcc ttttcctgac cattatacat acaaagataa ggatataaaa
      301 gaaatcgaaa gagaggcgct ggacaggaaa gcagatttca tattaactac tgaaaaggat
      361 ggggttcgtc tgattttgtt gccgtattcg aaagtgccga tttatattgt gaaaatgaaa
      421 cttgaaataa tttctggcga aacaagcttg tgggatttga tatatggaga atgaactcat
      481 aaaaacagat tttctggtaa tcggctcagg tatggctggt ttatcctatg ccttgaaggt
      541 atctggtttg ggaagcgtag ccattatcac caaaaagggg gagtgcgaat caaacaccaa
      601 ttatgctcag ggagggatag ctgcagttct ctcctccaac gattctttcc aactgcatat
      661 cgaggatacc atcaaagtcg ggatggaatt atgcaaggag gaagtggtag ccagagtggt
      721 ggaagcaggt ccggattgta ttgaggggct aattgagctg ggagtacatt tctccggcac
      781 gaaagggaaa ttcgacttgg gcatagaggg tggtcattca aagaaaaggg tagttcacgc
      841 caaagattat actggaagag aagttgaaga taacttactt aaagcagtta ggtccaaaca
      901 aaatataaca gtttatgaaa atcatttagc cattgatttg ataacccaac atcacttgaa
      961 gggatacaat ccaaagccag atgagaaagt gaactgctgg ggagcttacg ttttggacat
     1021 aaagtcaaac cggatcaaaa gatttctggc taaaactacc cttttagcta caggcggagc
     1081 aggcagggtt tatttgcata ccaccaatcc atccattgcc accggggatg gggtggctat
     1141 ggcttatcgt gcaggaacaa gagtcgctaa tatggagttc attcagtttc atcccacctc
     1201 gctttatcat tcgcaagcag agtccttttt gatatctgaa gcggtcagag gggaaggcgg
     1261 aaaattaaaa ctgaaatccg ggcagacctt tatgcacaag taccatccca aagccgaact
     1321 tgctccccgg gatgtggttg ctcgtgccat tgatactgag ctgaaggcaa gcggtgattt
     1381 ctgtgtttat ctggatatca cccatctgga tgcagatttc atacagaaga gatttcccaa
     1441 tatttataag cactgtcacc agttgggatt ggacataacc aaagagccga ttccggttgt
     1501 gccggcagcc cactatatat gcggaggagt aataactgat attcagggga agagtgatat
     1561 tcaagggctt tatgcctgtg gagaggttgc ctgtactggt atgcacgggg ctaatcgctt
     1621 agcctcaaat tctcttctgg aggcagtggt ctttgcccat ttcgccgcgg agagctctaa
     1681 ggaggagttc aagaaagatt tttcttttcc tgaaattccc ccctggagcg aagagggggt
     1741 ctttgaccaa aaagaatggg tcatcatctc gcataaccgc agtgaaatag aaaaggcgat
     1801 gtgggactac gtgggcatcg ttcgctcaaa tagccgtctg gaaatggcta agtcccgcat
     1861 agatactctt cttaaagaga taaacgagtt ttacaatctg aatccggtaa cctatgatgt
     1921 catagagctg agaaatgtat cgctggtagc tgacctgatt gtaagttctg ccttgatgcg
     1981 aaaggaaagc agggggttac attacaactt agattatcca caaatagacg atacaaattg
     2041 gcgcaaagac actatattac aacagaaggt ttggtaatga aaagtaaatc tcaagaaaga
     2101 attctgattt tggatttcgg ctcgcaatac actcaactta ttgcccgaag gataagggag
     2161 cttggggtct tctgcgagat agcccccttt aatcaggata tgtcctctta tggggacaaa
     2221 aatttaaaag gggttgtgct atccggtggt cctgccagcg tgttcgataa gaatgcccct
     2281 ttctgcaaaa agggtatttt tgagatgggg gtgccggttt tgggcatctg ttatgggctt
     2341 cagcttattg ctaaaatatt tgagggaaaa ctcgaaaagg cgaaaaacag ggaatatggc
     2401 agggcggttt taaagataga ttatgcggat atgcttttga aagatgtaaa gaatagcagt
     2461 gtggtgtgga tgagtcatgg ggataatgtg attcgagttc caaagggata taagatcttg
     2521 gctcatactg aagatatacc ctatgcggcc atggccaatt taaagcaaag aatcttcggc
     2581 ttgcaattcc atccggaagt ggcgcatact caagagggta aaaagatact gaagaatttt
     2641 ctatttacta tatgcggatg taaaggaacc tggactcctg cctcattcat aaattcccag
     2701 ataaaagaga taaaaaagaa agtgggtaaa gacaaggtgt tatgtggtct gagcggaggg
     2761 gtagattctt cagtctgcgc tgttctcata aacagagcta ttggaaaaca gcttaattgc
     2821 atctttgtga acaacggttt attaagggag ggtgaagctg gggaagtagt tgagactttc
     2881 aaaaaattga aaatgaattt aactttcgtg gatgccaagg ataaatttct ctccgccctg
     2941 aagggaataa ccgacccaga agaaaagaga agaaggatag gtaaattatt tattcaagtg
     3001 ttcgaagtcc aggctaaaaa aatcggaaaa acaaaatttt tagcccaagg aactctttac
     3061 ccggatgtta ttgaaagtac ctcttctaga ggaccatccg ccaccataaa atcgcaccac
     3121 aacgtgggtg ggttgccagc caaaatgaaa cttaagctga ttgagccatt aagggagctt
     3181 tttaaggatg aagtcagaga gataggcaga aaattagacc tgccggaaaa gataatagga
     3241 aggcaccctt ttccaggtcc aggactggcg gtcagagtct taggagaggt gactgaagga
     3301 agattgagta ttctgagaaa ggcggacagg atattcatac agaatttaaa ggagtccgga
     3361 ttttataata gagtctggca ggctttctgc gtgcttttgc ctatccaaac cgtaggagtt
     3421 atgggagacg agagaactta cgagaatgta gttgccttaa gggcggtaac cagcttagac
     3481 gggatgactg ctgactgggc ccgtctccct tatcatttag ttgagaaaat atccaaccag
     3541 ataataaatc aggttaaagg ggtgaatcgg gtagcctacg atgtaagctc caagccacct
//
LOCUS       DASWLN010000045         2016 bp    DNA     linear   ENV 20-FEB-2024
DEFINITION  MAG TPA_asm: Candidatus Zixiibacteriota bacterium isolate
            SMAG_U2949 k141_937025, whole genome shotgun sequence.
ACCESSION   DASWLN010000045 DASWLN010000000
VERSION     DASWLN010000045.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37960594
            Sequence Read Archive: SRR10376091
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Candidatus Zixiibacteriota bacterium (soil metagenome)
  ORGANISM  Candidatus Zixiibacteriota bacterium
            Bacteria.
REFERENCE   1  (bases 1 to 2016)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 2016)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (26-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/12/2024 08:28:26
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 1,440
            CDSs (total)                      :: 1,416
            Genes (coding)                    :: 1,412
            CDSs (with protein)               :: 1,412
            Genes (RNA)                       :: 24
            rRNAs                             :: 1 (16S)
            partial rRNAs                     :: 1 (16S)
            tRNAs                             :: 21
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 4
            CDSs (without protein)            :: 4
            Pseudo Genes (ambiguous residues) :: 0 of 4
            Pseudo Genes (frameshifted)       :: 0 of 4
            Pseudo Genes (incomplete)         :: 3 of 4
            Pseudo Genes (internal stop)      :: 1 of 4
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2016
                     /organism="Candidatus Zixiibacteriota bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_937025"
                     /isolate="SMAG_U2949"
                     /isolation_source="soil"
                     /db_xref="taxon:2053527"
                     /environmental_sample
                     /country="USA: Oregon"
                     /lat_lon="42.99 N 123.63 W"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            345..1127
                     /gene="fliR"
                     /locus_tag="VGB16_01795"
     CDS             345..1127
                     /gene="fliR"
                     /locus_tag="VGB16_01795"
                     /inference="COORDINATES: protein motif:HMM:NF013478.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0016020 - membrane [Evidence IEA];
                     GO_process: GO:0006605 - protein targeting [Evidence IEA];
                     GO_process: GO:0044780 - bacterial-type flagellum assembly
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="flagellar biosynthetic protein FliR"
                     /protein_id="HEX9916445.1"
                     /translation="MISIFDYTAQQLQTFLLILLRVSGILIAAPIFGHQAVPRQIKIG
                     LALILSFILVPLASTVTVNFPQEIIPLGFFLAKELIAGLFIGFAFLLIFLGVQMGGSL
                     VGLQIGFGLVSVFDPQSQEQTSVIGELQFLLAMLFFLAINGHHILISSIFQSFKIIPP
                     GAIGFSSLAAEKMVSMMAMSFVIAIKIAAPLMVTLFLTDVALGIIARTMPQMNVFIVG
                     FPLKIGVGLVMLVFSLPLFSLILEKLIMNLDLDVGWLINYLS"
     gene            1147..>2016
                     /locus_tag="VGB16_01800"
     CDS             1147..>2016
                     /locus_tag="VGB16_01800"
                     /inference="COORDINATES: protein motif:HMM:NF013479.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="EscU/YscU/HrcU family type III secretion system
                     export apparatus switch protein"
                     /protein_id="HEX9916446.1"
                     /translation="MAEGAEEFQEKTEEATPRHREESRKKGQVTKSMELSSAALIFFC
                     FLILNFLAPYIYHELSNFMVGTLDSMNLINLTMGNFHTLLLFWMLAFAKIVIPVVLAL
                     AVVALLVNFAQVGIVFSAEPLTPKLDRLNIAKGIKRILSKQSLFNLIRDVVKLVIIGY
                     VSYVTIKAEMNNYISLADKDVWQILVFASRMVFRVGMRTALALIILSVLDYAFQKWQF
                     EKGLKMTKDQLKEELKMTEGDPLIRSRIRRVQREMARRRMMKEVPKADVVVTNPTSIA
                     IALKYDTQTMSAPT"
BASE COUNT          548 a          424 c          448 g          596 t
ORIGIN      
        1 tatctttgta ggggcaattc atgaattgtc cctacgctct cacctttcca tctgctcgaa
       61 aattagccag ctgttcaaaa gttaaatcct gcacttaacc ggtgttccca tataatctta
      121 agaaaggaaa ggttatccaa aatagaggaa atattttcca atagagcaaa gactaacata
      181 ctctcataaa aaaatacttt cctcctaagt cataacacaa taaaaactta cctgtcatct
      241 acaaagattc catacaatag gcataagatt tgctcaacac ttgagtgaat ttcaacccac
      301 aataatttcg cacaagcttt accagtaaga actgaaagaa ttagatgatt tcaattttcg
      361 attacactgc ccaacagtta cagacatttc ttttaatcct cttgagagta agcgggattt
      421 tgatcgcggc acccattttt gggcatcagg ctgttccacg acaaatcaag atagggttag
      481 ctttgatttt gtccttcatc ttggtccctc tggccagcac agtaacagtg aactttccac
      541 aggagatcat acctttggga tttttcttgg ctaaagagct tatagccgga ctatttatcg
      601 gtttcgcttt tctcttgata tttttgggag tgcagatggg tggatcactt gtgggactac
      661 aaatcggttt tggtttggtg agcgttttcg accctcaatc tcaggaacaa acatcagtta
      721 tcggtgagct gcagttttta ttggcaatgc tattcttctt ggcaatcaac gggcatcaca
      781 ttttaatctc aagcattttc cagagtttca agatcattcc tccgggggca attggattct
      841 cctccctggc agctgagaag atggtcagta tgatggccat gtcgtttgtc atcgccataa
      901 agattgccgc acccttaatg gtaacgcttt tcttaacaga tgtggctttg gggataatcg
      961 cccgcaccat gccgcagatg aacgtgttca ttgtcggttt cccgttgaag atcggagtcg
     1021 gtctggtgat gctggtcttc agcttgccct tgttcagttt gatcttggag aaattgatta
     1081 tgaatctgga tttggatgtt ggttggctga ttaactattt gagttagatg agttagaaag
     1141 gaaattatgg ccgaaggagc tgaagagttc caggaaaaaa ccgaagaagc aacacccagg
     1201 catcgcgagg agtccagaaa aaagggacag gtgacgaagt cgatggagct cagctcggcg
     1261 gctttaatct tcttttgttt tctaatcctc aatttcttgg ccccatatat ctaccacgaa
     1321 ctctcaaatt ttatggtcgg aaccttggac agcatgaact tgattaatct gacaatgggg
     1381 aattttcaca ccctgctttt attctggatg ctcgcttttg ccaaaattgt aattccagtt
     1441 gtcttagccc tggcggtggt tgcccttttg gtcaatttcg cccaggtggg gatagtcttc
     1501 tcagctgagc cactaactcc caagttggac cgattaaata tcgccaaagg gataaagcgc
     1561 atcttatcca agcaatcgct tttcaatcta attcgggatg tggttaagct ggtgataatc
     1621 ggttacgttt cctacgtcac catcaaagcg gagatgaata actacatctc tttggcggac
     1681 aaggacgtct ggcagatttt ggtcttcgcc tctaggatgg tatttcgagt gggtatgcga
     1741 accgccttag ctctgataat cttatccgtt ttggattatg ctttccagaa gtggcagttc
     1801 gagaaagggt taaagatgac caaagatcaa ctcaaggaag agttgaaaat gactgaggga
     1861 gacccgttga ttcgctccag aataagaaga gttcaaaggg agatggccag aaggagaatg
     1921 atgaaggagg ttcccaaggc agatgtggtg gtgaccaatc caacttccat tgcaattgcc
     1981 ttaaagtatg atacccaaac tatgtctgcc cccaca
//
LOCUS       DASWLN010000046         4525 bp    DNA     linear   ENV 20-FEB-2024
DEFINITION  MAG TPA_asm: Candidatus Zixiibacteriota bacterium isolate
            SMAG_U2949 k141_1164894, whole genome shotgun sequence.
ACCESSION   DASWLN010000046 DASWLN010000000
VERSION     DASWLN010000046.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37960594
            Sequence Read Archive: SRR10376091
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Candidatus Zixiibacteriota bacterium (soil metagenome)
  ORGANISM  Candidatus Zixiibacteriota bacterium
            Bacteria.
REFERENCE   1  (bases 1 to 4525)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 4525)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (26-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/12/2024 08:28:26
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 1,440
            CDSs (total)                      :: 1,416
            Genes (coding)                    :: 1,412
            CDSs (with protein)               :: 1,412
            Genes (RNA)                       :: 24
            rRNAs                             :: 1 (16S)
            partial rRNAs                     :: 1 (16S)
            tRNAs                             :: 21
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 4
            CDSs (without protein)            :: 4
            Pseudo Genes (ambiguous residues) :: 0 of 4
            Pseudo Genes (frameshifted)       :: 0 of 4
            Pseudo Genes (incomplete)         :: 3 of 4
            Pseudo Genes (internal stop)      :: 1 of 4
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..4525
                     /organism="Candidatus Zixiibacteriota bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_1164894"
                     /isolate="SMAG_U2949"
                     /isolation_source="soil"
                     /db_xref="taxon:2053527"
                     /environmental_sample
                     /country="USA: Oregon"
                     /lat_lon="42.99 N 123.63 W"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            complement(175..1122)
                     /locus_tag="VGB16_01805"
     CDS             complement(175..1122)
                     /locus_tag="VGB16_01805"
                     /inference="COORDINATES: protein motif:HMM:NF042697.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF6599 family protein"
                     /protein_id="HEX9916447.1"
                     /translation="MRKLRLDLFLGFLISLLFYPSAQANCLENEKLLPEIIGGWRACD
                     SSSCYDRENLFDYIDGGAEIYLAYDFKSLTVQKYCRDTTQIIVEIYQTETLADAFGLY
                     SQSSMKRNITFAQRGGYESGELRFRKGAYFVRIFNWKEKDNLKKEILELGRRIAGKMK
                     GEEGLPVLFCALPKLGLVSGSERYLHQQISLNRTYFISNQNILNLNFKTNVVIADYRM
                     QGDSLKYLLIQYPNGVEAERAWQSFNRSYAKGKLKPYSSVVGLEIGEFIGAFLIKRYL
                     GIVLEGKKKEGALYLLGLSQSELEQVLREGKLPLKGVTN"
     gene            complement(1190..1921)
                     /locus_tag="VGB16_01810"
     CDS             complement(1190..1921)
                     /locus_tag="VGB16_01810"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_018206332.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0016020 - membrane [Evidence IEA];
                     GO_process: GO:0017004 - cytochrome complex assembly
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="cytochrome c biogenesis protein CcdA"
                     /protein_id="HEX9916448.1"
                     /translation="MLNISILTAFLAGIMSFISPCVLPLIPAYLSFISGFSIEEMQNG
                     ANRWKILKKVSLNSLFFILGFSFVFVAMGASATFLGSLLRSHISLFTKIAGIIIVAFG
                     LHLLGVFRIPWLYYEKRFHTQGRTLGIITPFILGLAFAFGWTPCVGPILAGMLLLAST
                     QASVFKGMLLLAFYSLGLGIPFFVAGLGFNLFIDLFERFKKSLRMIEMISGLFLVLIG
                     VLLFLDSFRLISEFLYRVLPWLTVG"
     gene            complement(2116..3219)
                     /locus_tag="VGB16_01815"
     CDS             complement(2116..3219)
                     /locus_tag="VGB16_01815"
                     /inference="COORDINATES: protein motif:HMM:NF012301.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003677 - DNA binding [Evidence IEA];
                     GO_process: GO:0000160 - phosphorelay signal transduction
                     system [Evidence IEA];
                     GO_process: GO:0006355 - regulation of DNA-templated
                     transcription [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="response regulator transcription factor"
                     /protein_id="HEX9916449.1"
                     /translation="MSMKILVIDDSPTMRSVAESSLRQKGYEVMLADGIQRGLELMQS
                     NKPDFILLDYSLPDIDCGAMCRRLRSEEQSKDVPLVVLLSSAELNVKAELTECGADGF
                     IVKPFTAKELLSTVEEVTAKRSPQGQATEQEGGLSQPSEGDKLSDSDKIEFDAMLIDS
                     SKKPKSPQKDTFEESSVSLDLSSIHASGPIEENVVSPDSLSDSSKIKTDSDFSLSHDY
                     NWFVSEIKKEMKENGEEKLQPETEIISESTSIDYMSKINLEDESKSAVEKTETPTDKE
                     FEHFLSDFRKEMEGVDIGEESPGVTAKEKEKPQEAKPRSEEAEAAHQEKLDHNKLADR
                     IVEDISEKIAQQIASKIDRDFLKEMIEKYLKEK"
     gene            complement(3254..4096)
                     /locus_tag="VGB16_01820"
     CDS             complement(3254..4096)
                     /locus_tag="VGB16_01820"
                     /EC_number="3.4.21.-"
                     /inference="COORDINATES: protein motif:HMM:NF013827.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004252 - serine-type endopeptidase
                     activity [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="rhomboid family intramembrane serine protease"
                     /protein_id="HEX9916450.1"
                     /translation="MSNSEVTDEPGYGRMTRIVKLLLIVNTAFLLLKFIGGSVIWQQT
                     LHWLGLTPTMVRQGDIWQLFTYMFLHGGFFHLLFNMFTLWMFGCEIERSWGSREFLKY
                     YTITGVGAGILTVVLTFILPLIPKLPQIDPAIPTIGASGAIFGILVAYALMFPNRLIY
                     IWFLFPVKAKYLVAFFAVFTLIASFRYSGGGVAHFAHLGGMVVGYLYIKADWKLASIS
                     HFLRELRYKRRLTKVTKDKQEEMDIMEQVDRVLDKINAHGMDSLTNREKKLLEKASHL
                     LSKR"
     gene            complement(4200..>4525)
                     /locus_tag="VGB16_01825"
     CDS             complement(4200..>4525)
                     /locus_tag="VGB16_01825"
                     /inference="COORDINATES: protein motif:HMM:NF014608.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=3
                     /transl_table=11
                     /product="OsmC family protein"
                     /protein_id="HEX9916451.1"
                     /translation="SKGPSPMEIVALALGGCTGMDIVNILKKRKVELRDFQINITSER
                     KEEEPRVFSKIEVEYLIGGEGIKEEDVALAVNLSKDKYCSVGKMLEGTAEIRYKWKIV
                     TDKLS"
BASE COUNT         1274 a         1123 c          825 g         1303 t
ORIGIN      
        1 tgactttacg agtaattgcc accggttctg cgtccgtgac cactgctaaa ttggggtagg
       61 ggttcaacgt atcagccaga actttggacc tcggcagttc agaaagactg gccagagcca
      121 aagtggcgat agagctttgg atgaactggc gacgagtgaa cctgtttttc gccatcaatt
      181 cgtaactcct ttcaagggga gcttcccctc ccttaggact tgctctaatt cactttggga
      241 cagccccaaa agatatagag caccctcctt ctttttgccc tctagcacta ttcctaaata
      301 tcttttgatt aaaaaggcac cgatgaattc accgatttca agtccaacaa cactcgaata
      361 cggctttaat ttccccttgg cgtaagaccg attaaagctc tgccaagccc tttcagcctc
      421 aaccccattt ggatattgaa tcaaaagata tttcaaagaa tcaccctgca ttctataatc
      481 cgcaataacc acgttcgtct tgaagtttaa gtttaaaatg ttttgattgg atatgaaata
      541 tgttctgttc aatgaaatct gctggtgaag atagcgctct gaaccagaga ccaaacccag
      601 cttgggtaat gcacagaaaa gaactggaag cccttcttca cctttcatct ttcctgctat
      661 cctcctccct aactccaata tctctttctt gaggttgtct ttttctttcc agttaaatat
      721 gcggacaaaa taagctcctt ttcggaacct caattccccg gattcataac cccctctttg
      781 agcaaatgtt atgttccgtt tcatagagct ctgggagtac aagccgaaag catctgctag
      841 tgtttcggtt tgatagattt ccactattat ctgggttgtg tctctgcagt atttctgaac
      901 agttagactt ttgaaatcat aggccaaata aatctctgcc ccaccatcaa tataatcgaa
      961 aaggttctca cgatcataac aggaggaaga atcgcacgcc ctccagccgc ctattatctc
     1021 cggcaacaac ttctcgttct ccaaacaatt ggcttgggca gatggataaa acaataacga
     1081 aatcagaaaa ccgagaaaca gatctaatct gagtttcctc atattctgta aatataatag
     1141 aagttgactc ttaaaaaaac aactttttca catagcgcta aagagcggtt taaccaactg
     1201 tcagccaggg gagcacccta tataggaact cggagataag tctgaaggag tcgagaaaaa
     1261 gcaaaacgcc aatcagaacc agaaataatc cgcttatcat ttcaatcatc ctcaagctct
     1321 ttttaaatct ctcaaaaagg tcgatgaaca gattgaatcc taaacctgcc acaaaaaagg
     1381 ggattcccaa tcccaaagaa tagaaagcca aaagaagcat tcccttgaaa acgctggctt
     1441 gggtggaagc aagcaaaagc attccagcta agatcggacc aacgcaagga gtccaaccaa
     1501 aagcaaaagc cagtcctagg ataaatggtg ttatgatgcc taaagttctt ccctgagtgt
     1561 gaaatctctt ctcgtaatag agccaaggaa ttctgaaaac gcccagaaga tgcaatccaa
     1621 aagccactat tattatgcct gctattttgg taaaaagtga gatgtgtgaa cgcaataaac
     1681 ttcccaagaa agtggctgaa gcccccatag ccacgaaaac aaatgagaac cccaagatga
     1741 aaaataaaga attaagactt actttcttta atattttcca cctatttgct ccgttttgca
     1801 tctcctctat ggaaaaaccg gaaataaacg aaagataagc cggaattagc ggcagaacac
     1861 aaggggagat aaaggacatt atccctgcca aaaaagcagt taatatggag atgttcagca
     1921 taatttactg aagaaaattg ctgagttaac ccaatattcc cttggataac tcaattggat
     1981 tatttgaatt actcgcctcc gccagaaagt ctcgctcgcc tctcggcgct cgcctctcgg
     2041 cgaagccgag ggaagccgag ggaagccgag ggccttttat attttggaag ccccgtcatt
     2101 tatcaccggg aggcttcact tttcctttag atatttctct atcatctctt tgagaaaatc
     2161 gcggtctatc ttactggcta tctgttgagc tattttttca gatatgtctt ctacgattcg
     2221 gtcagccaat ttattatgat cgagtttttc ctggtgagct gcctctgctt cctcacttct
     2281 tggcttcgcc tcctggggtt tttctttctc tttggcagtg acacccggac tctcctcacc
     2341 aatgtccact ccctccatct cctttctaaa gtcggacaga aaatgctcga attccttgtc
     2401 cgtcggtgtc tctgtttttt ctacggctga ttttgactca tcttctaaat tgatcttgga
     2461 catataatca attgaggtgc tctcgctaat gatttctgtt tctggttgta gcttctcctc
     2521 accattttct ttcatctcct tttttatctc ggagacgaac caattatagt catgcgataa
     2581 gctaaagtcg gaatcggtct tgattttgga ggagtcagac aaactatctg gcgagaccac
     2641 gttctcttct attggtccgc tggcatggat actgctcagg tcaagagaaa cggagctctc
     2701 ttcaaatgta tccttctgcg gtgatttcgg cttcttcgag ctatctatga gcatggcatc
     2761 gaactctatt ttgtcgctat ccgaaagttt atctccttcg ctcggctggg acagccctcc
     2821 ttcctgctcc gtggcttggc cctggggaga cctcttggca gtgacctcct ctacggtact
     2881 taatagctct ttggcagtga atggcttaac gatgaaaccg tctgctccgc attccgtcag
     2941 ctctgcctta acattcaatt cggcagagct caaaagcacc accaagggca catcctttga
     3001 ctgctcctca cttcttagcc ttctacacat tgcgccacaa tctatatccg gcaaggagta
     3061 atccagcaaa ataaaatctg gcttgttaga ttgcatcaac tccaagcccc tctgtatacc
     3121 atccgccagc atgacctcgt atcctttttg acgcaatgaa gattcagcca cgcttctcat
     3181 agtggggcta tcatctatta ccaatatttt catcgacata ccaacctccc tggccgttgg
     3241 tccagcatca gcgtcatctc ttggacaaaa gatgactggc tttttccaga agcttctttt
     3301 ctctattagt taaactatcc attccatggg cattgatttt atctaatact ctatccacct
     3361 gctccattat atccatttcc tcttgtttat ccttggtcac ttttgtcaat ctccgcttgt
     3421 atctcagttc cctcagaaag tgggagatgc ttgccagttt ccagtcagct tttatataaa
     3481 gataaccgac taccatacct cccaggtgcg caaaatgagc cacaccgcct ccactgtacc
     3541 taaatgaggc gattaaagtg aatacagcaa agaaagctac caaatacttg gctttaactg
     3601 gaaaaagaaa ccagatataa atcaggcgat taggaaacat caaggcgtaa gccaccagta
     3661 tcccaaaaat cgctcctgaa gccccgattg taggaatagc tggatctatt tggggaagtt
     3721 tcggaatcaa aggtaagatg aaggttaaga caaccgtcaa gatgcctgct cctacaccgg
     3781 ttattgtata atattttaga aactctcgag aaccccagct cctctctatc tcgcatccga
     3841 acatccacaa ggtgaacata ttgaataaca gatggaagaa accaccatgc aagaacatat
     3901 aggtaaacaa ctgccagatg tctccttgcc tgaccatagt tggagttaaa cccagccaat
     3961 gcaacgtttg ctgccatatg acgctccctc caataaactt caagagaagg aaagcagtgt
     4021 tgactatcaa aaggagtttg actatcctgg tcattctgcc atatcccggc tcatctgtta
     4081 cttcagaatt agacatattt caccctatta tattctatcg gaatttaaaa cccaaacaaa
     4141 acccctttta aaaacaaaaa attagtcact tttaacagtg cctaattggc caatctccat
     4201 taagaaagct tatccgttac tattttccac ttgtatctta tctcggctgt cccctccagc
     4261 attttaccta cggagcagta tttatcctta gaaaggttaa ccgctagggc tacatcttcc
     4321 tcttttattc cctctccacc aatcaaatac tcaacttcta tcttggagaa aaccctgggc
     4381 tcctcttctt ttctttcaga tgttatgttg atttggaaat ctctcaattc cacttttctt
     4441 ttcttaagaa tattaactat atccatacct gtgcatccac ccaaagccaa agccactatc
     4501 tccattggag atggaccctt gcttt
//
LOCUS       DASWLN010000047         7674 bp    DNA     linear   ENV 20-FEB-2024
DEFINITION  MAG TPA_asm: Candidatus Zixiibacteriota bacterium isolate
            SMAG_U2949 k141_1164913, whole genome shotgun sequence.
ACCESSION   DASWLN010000047 DASWLN010000000
VERSION     DASWLN010000047.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37960594
            Sequence Read Archive: SRR10376091
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Candidatus Zixiibacteriota bacterium (soil metagenome)
  ORGANISM  Candidatus Zixiibacteriota bacterium
            Bacteria.
REFERENCE   1  (bases 1 to 7674)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 7674)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (26-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/12/2024 08:28:26
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 1,440
            CDSs (total)                      :: 1,416
            Genes (coding)                    :: 1,412
            CDSs (with protein)               :: 1,412
            Genes (RNA)                       :: 24
            rRNAs                             :: 1 (16S)
            partial rRNAs                     :: 1 (16S)
            tRNAs                             :: 21
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 4
            CDSs (without protein)            :: 4
            Pseudo Genes (ambiguous residues) :: 0 of 4
            Pseudo Genes (frameshifted)       :: 0 of 4
            Pseudo Genes (incomplete)         :: 3 of 4
            Pseudo Genes (internal stop)      :: 1 of 4
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..7674
                     /organism="Candidatus Zixiibacteriota bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_1164913"
                     /isolate="SMAG_U2949"
                     /isolation_source="soil"
                     /db_xref="taxon:2053527"
                     /environmental_sample
                     /country="USA: Oregon"
                     /lat_lon="42.99 N 123.63 W"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            complement(<1..704)
                     /gene="bamD"
                     /locus_tag="VGB16_01830"
     CDS             complement(<1..704)
                     /gene="bamD"
                     /locus_tag="VGB16_01830"
                     /inference="COORDINATES: protein motif:HMM:TIGR03302.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0019867 - outer membrane [Evidence IEA];
                     GO_function: GO:0005198 - structural molecule activity
                     [Evidence IEA];
                     GO_process: GO:0065003 - protein-containing complex
                     assembly [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="outer membrane protein assembly factor BamD"
                     /protein_id="HEX9916452.1"
                     /translation="MKRFWVCLVVFVFLLGCASKSKIAPRNAEEQFEIAKDKYQKKKY
                     DEAVTEFYKVIFSYPGASFIDSAQFHLGMCYYWQEDYSLAIAEFKKLLSSFPTSRLAD
                     EAEYFIPLCNYYMSLKAPLDQEDTKLAVEGFANFLDYYPDSPYVPRAKRYLLEARSKL
                     AEKAYRNGVLYIKLDQYPAALIYFNEVLDKYGDTKWAGKSIFRIAEVYQKQGERGKAL
                     EKYKELMQNYPQEELV"
     gene            complement(725..961)
                     /locus_tag="VGB16_01835"
     CDS             complement(725..961)
                     /locus_tag="VGB16_01835"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEX9916453.1"
                     /translation="MNAFFHLVNATNNEIVWQTGVEYKKLDKLGIDALKSAENKNLPF
                     LYADLPSSATRKYVEPALVTALVGGLVYISFANR"
     gene            complement(965..1369)
                     /locus_tag="VGB16_01840"
     CDS             complement(965..1369)
                     /locus_tag="VGB16_01840"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEX9916454.1"
                     /translation="MVDLRINSILLSLAMSLFLFRLSWAEEPKNNENFLREMIAQSLN
                     QSFSEAIPQMKDTVYLKEEEEHPALRLLREELVSYLMQAKKGVFLFDSTKINSPFSQN
                     ASILKYHLTELKISYPKAERRGFLGKRIVTRE"
     gene            complement(1362..2018)
                     /locus_tag="VGB16_01845"
     CDS             complement(1362..2018)
                     /locus_tag="VGB16_01845"
                     /inference="COORDINATES: protein motif:HMM:NF013618.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="transglycosylase SLT domain-containing protein"
                     /protein_id="HEX9916455.1"
                     /translation="MGLGVKLIRRAGIIVSRWLSLILVLIYLGQAGAIAYLVYDKLQT
                     ERILNQQKEKMKELEEKLKIYHIIEDFQVGFDRKEVGELAQVIHEESKKYGYDPLLLI
                     SIIITESSFRPGQVSYLGAEGLMQLKPSVGFELAQRRKLQWFGDNSLSIPTLNIQLGA
                     LYLFELILEFKDVKQALIAYNLGENNLRLCIKEGRALPDRFLFKVLSKYKELREKYNG
                     "
     gene            complement(2033..2878)
                     /locus_tag="VGB16_01850"
     CDS             complement(2033..2878)
                     /locus_tag="VGB16_01850"
                     /inference="COORDINATES: protein motif:HMM:NF015983.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003824 - catalytic activity [Evidence
                     IEA];
                     GO_function: GO:0051536 - iron-sulfur cluster binding
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="radical SAM protein"
                     /protein_id="HEX9916456.1"
                     /translation="MELCAYSTSRDIKIRQTKCRTALSPSKIEGVDYALNPYVGCEHG
                     CVYCYAVFMKRFSDHREEWGEFVDIKANFVEKLANQLKRAKPGVIMLGTVTDAYQPLE
                     REVGLSRKCLELLTEYDFEVHIQTKSDLVLRDLDLLKQMRNCHIGFTITTLDEKIASQ
                     FEPKASKVSQRLEALKVLSENGLDTFAFVGPILPSFSDNVLSLKKIFQKLKSLAIEKI
                     YVDRMHYWGDRWERIRSFIGECYPHLLDYYKWSKGNYEQYSAKLRDSVYRSLAGIDLN
                     CEILF"
     gene            complement(2888..4192)
                     /gene="rimO"
                     /locus_tag="VGB16_01855"
     CDS             complement(2888..4192)
                     /gene="rimO"
                     /locus_tag="VGB16_01855"
                     /EC_number="2.8.4.4"
                     /inference="COORDINATES: protein motif:HMM:TIGR00089.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008172 - S-methyltransferase activity
                     [Evidence IEA];
                     GO_function: GO:0016782 - transferase activity,
                     transferring sulphur-containing groups [Evidence IEA];
                     GO_function: GO:0051539 - 4 iron, 4 sulfur cluster binding
                     [Evidence IEA];
                     GO_function: GO:1904047 - S-adenosyl-L-methionine binding
                     [Evidence IEA];
                     GO_process: GO:0018339 -
                     peptidyl-L-beta-methylthioaspartic acid biosynthetic
                     process from peptidyl-aspartic acid [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="30S ribosomal protein S12 methylthiotransferase
                     RimO"
                     /protein_id="HEX9916457.1"
                     /translation="MSFINLGCPKNDIDSEIMQRLLAEQGYSLTDKLSDAGVFVLNSC
                     AFIQSAKEESIHKIMELIKLKEKNKKLIVAGCLAQRYAKNLWEEIPEIDGLLGINQLA
                     YVAKACNGVLKNKRYFKPFPQAKGKTWIETKRLKQKSPYAYIKIADGCSNNCAYCAIP
                     QIRGKYRSKPMELILREAEELVSYGTKEINLVAQDTTLYGIDLYGNKKLACLLDKLSG
                     IPQLKWIRLLYTHPAHFEDEMIHEIASNDKVCKYIDLPLQHISDKILSRMNRQVSSKE
                     VNLLIDKLRKNIRNLTLRTSLMVGFPGETEKDFKQLLEFVQKIRFDKLGSFAYSREEG
                     TKAYNLPRQLPTETKLERLDKLMLTQQEIFFEENQKKIGKKLEVLVEAEDDGYFVCRS
                     QAEAPEVDNAILVKSKKMKIGDFAKAEIIGSCAYDLIAQIGN"
     gene            complement(4241..4891)
                     /locus_tag="VGB16_01860"
     CDS             complement(4241..4891)
                     /locus_tag="VGB16_01860"
                     /inference="COORDINATES: protein motif:HMM:NF015510.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0009279 - cell outer membrane [Evidence
                     IEA];
                     GO_process: GO:0042953 - lipoprotein transport [Evidence
                     IEA];
                     GO_process: GO:0044874 - lipoprotein localization to outer
                     membrane [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="outer membrane lipoprotein carrier protein LolA"
                     /protein_id="HEX9916458.1"
                     /translation="MSKEKRLLFFLSIFVASAFSPKAYPLTVEDLMGQLNNKYSSFED
                     VRMEFTQSISSDVFESKSHFQGQIYIKNPDKFRIETLQQTIVANGEYIWVYSEENKQV
                     TKNRYDPSSGILLPYRYLNSFKDDYKAKLDKEELIKKRWCYKLVLTPKDENSFVTKMI
                     IWVDKETLLTLKLEYWDLNDNEVTFLFKNIRIDSKIDDSEFVFKIPPGIELLDLSE"
     gene            complement(4884..7097)
                     /locus_tag="VGB16_01865"
     CDS             complement(4884..7097)
                     /locus_tag="VGB16_01865"
                     /inference="COORDINATES: protein motif:HMM:NF013727.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003677 - DNA binding [Evidence IEA];
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_function: GO:0015616 - DNA translocase activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="DNA translocase FtsK"
                     /protein_id="HEX9916459.1"
                     /translation="MPIFRDLYHKRKNEILGLLLIALGLMLLISLLTYDAQRDLKYLK
                     SQMSLGNLFTTRVNNAAGIVGLLVSYYLYYLLGVCALAVPLFTLAWGANRLFKWDPIW
                     LLKKLLYLCLFFFLLGSTFFVSTVAGNRAWSFSRVEVGGWLALNFAKLWVVILGHIGS
                     YLFLITLLIIIIVLITPWGFGDLWLAIKPFLSSLVKRGGHVAEMRRIKKQRVKREKQV
                     REELEEKSIGEQIEAPQVESNAKSLKLVQNKYQPKEGGEYRFPTLDLLDEPSYAKSSV
                     SDEELNNTAKALRESLATFDIGIEEDFIEKSPGPIITRYEFKPAPGIKVNQIVNLADD
                     LALALQARRVRVVAPIPGKAAVGVEIPNKTPQTVYIKDILTCTGFQNEKLRLPIGLGK
                     TISGAPFVADLAKMPHLLIAGATGSGKSVCINVIISSLLYRLHPEEIRFIMIDPKMLE
                     LTVYDGIPHLEGEVLTSPKKAEKVLADVVVEMEERYRRLAKAGVRNIEDYNQKKGEDG
                     ILPYIVIVVDELADLMMSSSTRTEALITRLAQMARAVGIHLILATQRPSVDVITGLIK
                     ANFSARIAFQVASKIDSRTIIDIGGAEKLLGNGDMLFVQTGHPEPRRIHGAFISSPET
                     ERVVDFIKLQNYRVSPTEIFAREEEEKRVFQEEEERNDDLLMRAAELVVRHKQGSVSL
                     LQRRLGVGYQRAARLIDQLEEYGVVGPYDGSKAREVLVDANYLESVEFLKRRKVE"
     gene            complement(7256..7531)
                     /locus_tag="VGB16_01870"
     CDS             complement(7256..7531)
                     /locus_tag="VGB16_01870"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_013247007.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003677 - DNA binding [Evidence IEA];
                     GO_process: GO:0030261 - chromosome condensation [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="HU family DNA-binding protein"
                     /protein_id="HEX9916460.1"
                     /translation="MTKEELAGKIAKECKISKTQAHKAINSFTDGVTKALKMGKKVSL
                     VGFGTWSVGKRKARIGRNPQTGATIKIAAARVPKFKAGKQLKKAVKY"
BASE COUNT         2070 a         1884 c         1381 g         2339 t
ORIGIN      
        1 ttcaccaact cctcctgagg gtaattctgc attaactctt tatatttctc taaagcctta
       61 ccccgctcgc cctgcttctg gtaaacctca gctattctga aaatcgattt gcccgcccat
      121 ttggtatcac catatttgtc caaaacttca ttaaaatata tcaaagcagc cggatattga
      181 tccagtttaa tatagagaac accatttcgg taagccttct cagccaattt gctacgcgct
      241 tcaagcaggt atctttttgc ccgtggaaca taagggctgt ctggataata atccaggaaa
      301 ttagcaaacc cctccacagc taatttggta tcttcctggt ccagtggtgc tttaagggac
      361 atataataat tacataaggg gatgaaatat tcggcttcat ccgccaatct gctggtggga
      421 aaactgctca gcaatttctt gaactcagct atagctaagg agtagtcctc ctgccaatag
      481 taacacatac ccaaatggaa ttgtgcggaa tctatgaagc tcgctccggg atagctgaaa
      541 atgactttat aaaactcagt taccgcttca tcgtatttct tcttttgata tttgtctttg
      601 gcgatctcga actgctcctc agcattgcga ggcgctatct tgctcttgga ggcgcatcct
      661 aaaagaaaaa caaaaactac cagacagacc caaaacctct tcatcttttg tcctttccta
      721 aagtctaacg attggcgaaa gatatataaa ccaatccccc caccagtgca gtgactaaag
      781 ctggctcaac atacttccga gtagcgctgg agggtaaatc cgcatacagg aatggcaaat
      841 tcttattttc tgccgatttt agggcatcta taccaagttt gtccaacttt ttatattcca
      901 ccccggtttg ccagactatt tcattgtttg tggcattcac taagtgaaag aaagcgttca
      961 cctttcactc acgagtaaca atcctcttac ccagaaagcc ccttctttct gctttggggt
     1021 aactgatttt aagttcagtt agatgatatt ttaatatgct tgcgttctgc gaaaaaggag
     1081 agtttatctt agttgaatca aacaggaaaa ctcccttttt ggcctgcatc agatagctaa
     1141 ccagttcctc ccttagaagc cgcaaagctg ggtgctcttc ttcttctttg agatataccg
     1201 tatccttcat ctgcgggatg gcctctgaga aggattgatt caaggattga gcaatcattt
     1261 ccctcaagaa attttcatta ttctttggct cctctgccca actcaagcga aacagaaaca
     1321 aactcattgc caaagacagg agaattgaat tgattcttaa atcaaccatt gtatttttct
     1381 cttaactcct tatatttgct caatactttg aataagaacc tgtctggcaa agctctgcct
     1441 tccttgatgc ataatctcag attgttttca cctaaattat aggcgattag ggcctgcttc
     1501 acatccttga attccaaaat caattcgaaa agataaagtg ccccaagttg tatgttgaga
     1561 gttggaatgg aaagcgaatt gtctccaaac cattgcagtt ttctcctctg agctaattcg
     1621 aagccgactg acggcttgag ctgcatcaag ccctctgctc ctagataaga aacctgcccc
     1681 ggcctgaatg agctttcggt tatgattatg gatatgagga gaagcgggtc atagccatat
     1741 ttcttgcttt cctcatgaat cacctgggct aattctccga cttccttacg gtcaaaaccg
     1801 acctggaaat cctcaatgat atgatagatt ttcaatttct cttccaactc tttcatcttt
     1861 tctttctgct ggtttaaaat cctttcggtc tggagcttgt cgtagaccaa gtatgcgatc
     1921 gcccctgcct gacccaaata aatcaagact aagattaaac tcagccatcg ggaaactatt
     1981 atgccagccc ttcttatcaa tttcactcct aaacccatcc gctcctcctt ttttagaaga
     2041 gtatctcaca attcaaatca attccagcca aactcctgta aactgaatct ctcaatttag
     2101 ctgaatactg ttcgtaattg cccttactcc acttatagta gtccaagagg tggggatagc
     2161 actcccctat aaaagacctt atcctttccc acctatctcc ccagtaatgc atgcggtcaa
     2221 cgtagatttt ttctattgcc aaacttttaa gcttttgaaa aatctttttc aaggataaga
     2281 cattgtctga aaatgatgga agaattggac caacaaaagc aaaggtatca agtccatttt
     2341 cagataagac cttcaaggcc tccaggcgct ggcttacctt agaggctttc ggttcaaact
     2401 gactggctat cttttcgtct aaggtggtta tggtaaagcc gatatgacaa ttcctcatct
     2461 gtttcaacaa atctaaatct cttaaaacca aatctgactt ggtttgaata tgaacttcaa
     2521 aatcatactc tgtcagaagc tctaaacatt tgcggctaag ccccacttcc ctttccagag
     2581 gttgataagc atcagttact gtgcccagca ttatcacgcc cggctttgcc cttttcaatt
     2641 ggttagccaa tttctcaaca aaattcgcct tgatatctac gaactcaccc cattcctctc
     2701 gatggtcgct gaatcttttc ataaaaactg cgtaacaata aacgcatcca tgctcacaac
     2761 caacataagg attcaaagca taatccacac cctctatttt ggaaggggaa agagcggtgc
     2821 ggcacttggt ctgtcttatc tttatatctc tacttgtcga atatgcgcaa agttccaaaa
     2881 ataacctcta atttccaatt tgggctatta aatcataagc gcaactgcca atgatttccg
     2941 ctttcgcaaa atcgcctatt ttcatcttct tgctcttgac taaaattgca ttgtcaactt
     3001 ccggcgcctc agcctggctt cggcaaacaa aatatccatc gtcctctgct tcaaccaaga
     3061 cctcaagctt cttccctatc ttcttctggt tttcctcaaa aaagatttcc tgttgggtta
     3121 gcatcagctt atccagacgc tctaatttgg tttcagttgg caactgcctt ggcagattat
     3181 aagctttagt tccttcctct cgcgaatagg cgaaacttcc gagtttgtca aatcttatct
     3241 tctgaacgaa ctccaaaagt tgcttaaaat ctttttcggt ctcccctgga aaaccaacca
     3301 taagagaggt gcggagggtc agatttcgta tattctttct caatttatcg attaggaggt
     3361 taacctcttt tgagcttacc tgacgattca tccgggatag aattttatct gaaatatgct
     3421 gtaaaggtaa atcaatatac ttgcatactt tgtcatttga agctatttcg tgaatcattt
     3481 cgtcctcgaa atgggcagga tgagtgtaaa gcaaacgaat ccatttgagc tgaggaatcc
     3541 ctgagagttt atccaaaaga caagctaatt tcttattgcc atacaggtca attccgtaca
     3601 gagttgtatc ctgggcaact aaatttatct cttttgttcc ataagagact agctcctcgg
     3661 cttctcttaa aatcaactcc atcggcttgc ttctgtattt tccccttatt tgaggaatgg
     3721 cacaataagc acagttgttg ctgcagccat ccgcaatctt tatgtaagca taaggggatt
     3781 tctgttttaa tcttttcgtc tcaatccaag ttttcccttt tgcttgagga aatggtttga
     3841 aatatctttt gttttttagt actccattac aggctttagc cacataagcc agctggttta
     3901 ttcccaaaag cccatctatt tcaggaatct cctcccacag gtttttggcg taacgttgtg
     3961 ctaaacaccc agctacgatg agttttttgt ttttttcttt taacttgatt agttccatta
     4021 tcttatggat tgattcttcc ttggcagatt gaatgaaagc gcaactgtta agcacaaaca
     4081 cccctgcatc acttagtttg tctgtcaggg agtatccttg ttctgcaagt agcctctgca
     4141 taatctcaga gtcaatatca tttttgggac agcccaaatt gatgaagctc acaagctctt
     4201 tcaagactat acctttgttt gtatatgata aatccagact tcactcgctt aggtccaaaa
     4261 gctcaattcc agggggaatt ttaaagacaa attcggaatc atctatctta gaatctatcc
     4321 tgatattctt gaaaagaaag gtgacctcat tatcattcaa atcccagtat tccaacttca
     4381 gagtcaagag agtctcctta tccacccata taatcatttt ggtaacaaaa ctgttttcgt
     4441 ctttgggggt tagaactaat ttgtagcacc atcttttctt aatcagctct tccttatcca
     4501 gtttggcttt ataatcatcc ttgaaactat tgagatatcg atagggcaag agtattccgc
     4561 ttgagggatc gtacctgttt ttagttacct gcttgttctc ttcggaatag acccagatgt
     4621 attcaccatt ggcaactatg gtttgctgga gggtttcgat tctgaacttg tccgggttct
     4681 taatataaat ctgcccttgg aaatggcttt tgctttcaaa gacgtccgaa cttatgctct
     4741 gcgtgaattc catcctcaca tcttcaaacg agctgtattt gttgttgagc tggcccatca
     4801 aatcctcaac tgtgagggga tacgcctttg gcgagaaagc actggcgaca aatatagata
     4861 aaaaaaataa tagccgtttt tccttactca acttttctcc tttttaaaaa ttccacactc
     4921 tccagataat tcgcatccac caatacctct ctggctttgc tcccatcata aggtcccacc
     4981 acaccatatt cctccaactg gtcaatcaag cgagcggccc tctgataacc aactcccaat
     5041 cttctctgta gaagcgaaac tgaaccttgc ttgtgccgaa ccacaagttc cgccgccctc
     5101 atcagaaggt catcattcct ttcctcttct tcctggaaaa ctctcttctc ctcttcctcc
     5161 cgggcaaata tctccgtggg cgaaacccta taattctgca gttttatgaa atccacgact
     5221 ctctccgtct cagggctgga aataaaagca ccatgtattc ttctgggttc cggatgtccg
     5281 gtctgaacga aaagcatatc cccatttccc agaagcttct ctgccccacc aatgtcaatt
     5341 atggtgcggg agtctatctt ggaagctacc tggaaggcaa tgcgagcaga gaaattggct
     5401 tttatcagtc cggttataac gtccactgaa ggcctttgag tggctaagat gaggtgtatt
     5461 ccaactgcgc gcgccatctg ggctaagcga gtgattaaag cttcagtgcg ggtggaactg
     5521 ctcatcatca ggtcagctaa ttcatccacc acgatgacga tgtaagggag tatgccatct
     5581 tctccttttt tctgattgta atcctcaata tttctaactc cagccttagc caatcttcta
     5641 tatctttcct ccatctccac caccacatcc gctaaaacct tctccgcttt cttgggactg
     5701 gttaaaacct ctccttccag atggggaata ccatcatata ctgttaactc cagcatcttg
     5761 gggtctatca tgataaatct tatctcttcc ggatgcaggc ggtagagcag actggagatt
     5821 atcacgttta tgcacacact ttttcctgag ccggtagctc cggcaatcaa aagatggggc
     5881 atcttggcta agtcggcaac aaagggagcg ccggagatgg tcttgcccaa tccgatgggc
     5941 agacgcagtt tttcattttg aaagccagta caagtcagaa tatcctttat atacacagtt
     6001 tgaggggttt tattcggaat ctcaactcca accgcagctt tacctggaat aggggcaacc
     6061 accctgaccc ttctcgcttg cagggctaag gctagatcat ctgccaagtt gactatctgg
     6121 ttaaccttta tccccggagc cggtttgaac tcgtagcggg ttataatcgg tcccgggctt
     6181 ttctcgatga aatcctcttc aatcccaatg tcaaaagttg ccaaactctc tcttaatgcc
     6241 ttggcggtat tgttcaactc ctcatctgaa actgaagatt tagcgtagct gggttcgtcc
     6301 aaaagatcta aagttgggaa acgatactcc ccaccctcct tgggttgata cttattttga
     6361 actaacttaa gagacttggc attactttcc acctggggtg cctcaatctg ttccccaatc
     6421 gatttttcct ccaactcttc tcttacctgt ttttctcttt tgactctttg ttttttaatc
     6481 ctccgcatct ccgctacgtg cccacctcgc ttgaccaagc tggaaagaaa aggcttaatc
     6541 gccagccata agtcgccaaa tccccaggga gtaatcaaga ctatgattat aatcaggagc
     6601 gtaatcaaga ataaataact acctatgtga ccaagtatga ctacccagag tttggcgaaa
     6661 tttagggcaa gccaaccacc cacctcaact ctggaaaatg accaggctcg atttcccgcc
     6721 accgtagaaa caaaaaaggt cgaacccaga aggaagaaga aaaggcaaag ataaagcagc
     6781 tttttcaaaa gccagatggg gtcccattta aacaagcgat ttgctcccca ggccagagtg
     6841 aataacggaa cagccaaggc gcaaacaccg agaagataat acaggtaata ggaaactaaa
     6901 agccccacta tgccagcggc gttattgacc cgggtagtaa agagattacc caagctcatc
     6961 tggcttttca ggtactttaa atccctttgg gcatcgtagg tcaaaaggct aatcaaaagc
     7021 atcaatccta aggcaattaa cagtagccct aatatctcat tttttctttt atggtaaagg
     7081 tctctaaaaa tcggcacaga ttcgtccttt tccttttcaa cttaaaaaat ttaatacatt
     7141 atttccaata gtcaacaaca aaaatggcaa ctccactgag ttgccgtact ttgccttact
     7201 taagaagaaa aggcaggttt taccctgcct tccacaagta gatgaaaccc cgattttaat
     7261 actttacagc tttcttcagc tgcttgcccg ctttgaactt cggcactctc gctgcagcga
     7321 tcttgatcgt cgctccagtc tgaggatttc tgccaatacg agcttttctc ttacctacgc
     7381 tccaagttcc aaaaccaacc agactcacct tctttcccat cttcaaagcc ttggttaccc
     7441 cgtccgtgaa agaattaatg gctttgtggg cttgggtttt gctgattttg cactccttag
     7501 caatcttacc agccagttcc tcctttgtca tctactcacc tccttaaggg cgaaagggtt
     7561 aattgtttta ggtgaacgcc ccaaaaatac aataaccatg cggttttgtc aagaactatt
     7621 ttcattttcg acgatttttt ttcaactccc gcccttccta acccctctaa aata
//
LOCUS       DASWLN010000048         4620 bp    DNA     linear   ENV 20-FEB-2024
DEFINITION  MAG TPA_asm: Candidatus Zixiibacteriota bacterium isolate
            SMAG_U2949 k141_683502, whole genome shotgun sequence.
ACCESSION   DASWLN010000048 DASWLN010000000
VERSION     DASWLN010000048.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37960594
            Sequence Read Archive: SRR10376091
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Candidatus Zixiibacteriota bacterium (soil metagenome)
  ORGANISM  Candidatus Zixiibacteriota bacterium
            Bacteria.
REFERENCE   1  (bases 1 to 4620)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 4620)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (26-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/12/2024 08:28:26
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 1,440
            CDSs (total)                      :: 1,416
            Genes (coding)                    :: 1,412
            CDSs (with protein)               :: 1,412
            Genes (RNA)                       :: 24
            rRNAs                             :: 1 (16S)
            partial rRNAs                     :: 1 (16S)
            tRNAs                             :: 21
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 4
            CDSs (without protein)            :: 4
            Pseudo Genes (ambiguous residues) :: 0 of 4
            Pseudo Genes (frameshifted)       :: 0 of 4
            Pseudo Genes (incomplete)         :: 3 of 4
            Pseudo Genes (internal stop)      :: 1 of 4
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..4620
                     /organism="Candidatus Zixiibacteriota bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_683502"
                     /isolate="SMAG_U2949"
                     /isolation_source="soil"
                     /db_xref="taxon:2053527"
                     /environmental_sample
                     /country="USA: Oregon"
                     /lat_lon="42.99 N 123.63 W"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            <1..1315
                     /locus_tag="VGB16_01875"
     CDS             <1..1315
                     /locus_tag="VGB16_01875"
                     /inference="COORDINATES: protein motif:HMM:NF016859.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=2
                     /transl_table=11
                     /product="DNA-directed RNA polymerase subunit beta'"
                     /protein_id="HEX9916461.1"
                     /translation="AARIEEQSKVIAKSEGKVTYKNIKKVRKKDSRVVVLGRDGEIVL
                     LDEKDRVRARYNVPYGATMKVGNNVLVAKGDVIFEWDPYASTIISEESGTVQFQDITE
                     GKSLREELDETTGLRQRVIIEQKDKTLRPNIAIVNDKGKKLAGYSIPTGAHLLVHDAD
                     EIEAGDALVKIPREISKTRDITGGLPRVAELFEARRPKDPAVVGELDGTIEFGKIVRG
                     STQIFVRGEAGEVREYLIPHGKHMRVHGGDKVKAGEKLCEGPIDPHDILRIKGPNAVQ
                     EYLVNEIQEVYRLQGVKINDKHIEVIVRQMLQKVRVEDVGDTNFLEGEFVDKNRFKEE
                     NEKVIVEGGEPATFQPLLLGITKASLSAESFISAASFQETTRVLTEAAINGKTDYLLG
                     LKENVIIGHLIPAGTGLERYREVVIKEEEPPEEARSEMEILQEKP"
     gene            1391..1774
                     /gene="rpsL"
                     /locus_tag="VGB16_01880"
     CDS             1391..1774
                     /gene="rpsL"
                     /locus_tag="VGB16_01880"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_009016331.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0000314 - organellar small ribosomal
                     subunit [Evidence IEA];
                     GO_component: GO:0022627 - cytosolic small ribosomal
                     subunit [Evidence IEA];
                     GO_function: GO:0003735 - structural constituent of
                     ribosome [Evidence IEA];
                     GO_process: GO:0006412 - translation [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="30S ribosomal protein S12"
                     /protein_id="HEX9916462.1"
                     /translation="MPTINQLIRKGRRIVRSKASTPALTGSPQKRGVCTRVYTSTPKK
                     PNSALRKVARVRLTNSFEVTAYIPGEGHNLQEHSIVLIRGGRVKDLPGVRYHIIRGTL
                     DATGVADRKQSRSKYGTKRPKIEKE"
     gene            1780..2250
                     /gene="rpsG"
                     /locus_tag="VGB16_01885"
     CDS             1780..2250
                     /gene="rpsG"
                     /locus_tag="VGB16_01885"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002614776.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0000314 - organellar small ribosomal
                     subunit [Evidence IEA];
                     GO_component: GO:0022627 - cytosolic small ribosomal
                     subunit [Evidence IEA];
                     GO_function: GO:0003735 - structural constituent of
                     ribosome [Evidence IEA];
                     GO_process: GO:0006412 - translation [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="30S ribosomal protein S7"
                     /protein_id="HEX9916463.1"
                     /translation="MSRKKGFTKRERLPDPKFKSVLVTQFINNLMRRGKRSLAENVFY
                     ETMDLINKKTSQPGLDVFNKALNNVKPLLEVKSRRVGGATYQIPIEVRADRRTALAIR
                     WVIGYAKERSEHTMPERLAAELVAASKNEGTAIKKKEDTHKMAEANKAFAHFRW"
     gene            2388..4469
                     /gene="fusA"
                     /locus_tag="VGB16_01890"
     CDS             2388..4469
                     /gene="fusA"
                     /locus_tag="VGB16_01890"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011393940.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003746 - translation elongation factor
                     activity [Evidence IEA];
                     GO_process: GO:0006414 - translational elongation
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="elongation factor G"
                     /protein_id="HEX9916464.1"
                     /translation="MVDRFPLDRVRNIGIMAHVDAGKTTTTERILYYTGKTHRIGEVD
                     DGAATMDWMDQEKERGITITSAATTCFWRDHQINIIDTPGHVDFTAEVERSLRVLDGA
                     VAVFCGVGGVEPQSETVWRQADRYRVPRIAFVNKMDRVGADFEMVLETMLERLKARAV
                     PIQLPVKKGDDFLGIIDLIQMNYRVYHADDLGRTFEDREIPKEFEQKSKKRREELLEA
                     LSDYDEQLLENFVHEKPIEIEQIKGALRKATLDTKLVPVLCGAALKNQGVQKLLDAVI
                     DFLPSPLDMPPVKGINPKNDQETQRKVADNEYFCALAFKVATDPYVGKVIYFRVYSGK
                     VSAGSQVLNSNTQQKERIVRILEMHADKREEKGEVHTGDIAAVVGLRNTTTGNTLCDP
                     KKPIILEKMSFPEPVISVAIEPKTKADQDKLDSALSKLMDEDPTFKVNFNEETGQTII
                     SGMGELHLEILVERMLREFGVGANVGRPQVSYKESITQTVESEGRFVKQTGGRGHYAS
                     VKIRLEPTERHKGLVFENKLKGGTIPKEFVPAIEKGIKESMESGVMAGYPVIDIKVTL
                     LDGSFHEVDSSEIDFKAAARDALVNGFTKANPILLEPVMKVEVVLPEEYMGEVIADLN
                     ARRGRVERTSRRADGWVVDAMVPLAEMFGYATILRSLSQGRAIYTMEFSHYEEVPEAI
                     SESLLTKIRGY"
     gene            4492..>4620
                     /locus_tag="VGB16_01895"
     CDS             4492..>4620
                     /locus_tag="VGB16_01895"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011416212.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003924 - GTPase activity [Evidence IEA];
                     GO_function: GO:0005525 - GTP binding [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="GTP-binding protein"
                     /protein_id="HEX9916465.1"
                     /translation="MSKKRFERTKPHLNIGTIGHVDHGKTTLTAAITMVLHRKNMAD"
BASE COUNT         1426 a          828 c         1281 g         1085 t
ORIGIN      
        1 tgcggctcga attgaggagc aatccaaagt aatagccaag agcgaaggga aggtaactta
       61 caagaacata aaaaaagtgc gcaaaaagga tagtcgagtc gtggtcttgg gtcgagatgg
      121 ggagatagtc ctcctggatg aaaaggacag ggtcagagcc agatataatg ttccctacgg
      181 agccacgatg aaagttggga acaatgtact agtggcgaaa ggagatgtaa tctttgaatg
      241 ggacccatat gctagtacca ttatttcaga agaatctggt actgttcagt tccaggatat
      301 caccgaggga aagagcttac gtgaggagtt ggatgaaacc actgggttaa ggcagcgggt
      361 cataatcgaa caaaaagata aaaccctaag acctaatatc gccatagtta atgacaaagg
      421 gaaaaagctg gctggttatt cgatacccac cggggctcat cttctggtac atgacgcaga
      481 cgagattgaa gccggagatg ccttggtcaa aatccctcga gagataagca agactcgaga
      541 cataactggt ggacttcccc gtgtggcaga gttgtttgaa gccaggaggc ccaaggatcc
      601 agcggtggtg ggagagttgg atggaacgat agaatttgga aaaatagtaa gaggctccac
      661 tcagatattc gttcgaggag aggcggggga agtcagggag tatttgattc ctcacggaaa
      721 acatatgcgg gtgcatggag gcgacaaggt taaggctgga gagaaactgt gtgaaggccc
      781 catagaccct cacgatatac tgagaatcaa aggccctaat gcggtgcaag aatatctggt
      841 caacgagatt caggaagtat acaggttaca gggagttaag attaatgata aacatataga
      901 agtgattgtg aggcagatgt tgcagaaggt tagggtggag gatgtgggcg acactaattt
      961 tctggaaggg gagtttgtgg acaagaatag gtttaaggag gaaaatgaga aagtcatagt
     1021 tgagggcgga gagccagcta ctttccaacc actgcttctg gggataacca aagcttctct
     1081 ttctgctgag agcttcatct cagctgcctc attccaggaa actaccagag tattaaccga
     1141 ggcagccatc aatggcaaga ccgactacct cttgggattg aaagaaaatg tcattatagg
     1201 gcatctcatt ccggcgggaa ctggattgga gagatatcgt gaagttgtaa taaaagagga
     1261 ggaaccgccg gaagaagcac gttccgagat ggaaatattg caagaaaagc cttgaccaaa
     1321 gcgacaatat gaggtataat aaaagtttaa cttgtatctt tttgcctttg actggaaatg
     1381 gaggagcgtc ttgcctacaa taaatcaact gataagaaaa gggagacgaa tagttcggag
     1441 taaggcaagc actccagccc ttacgggttc gcctcaaaaa agaggagtgt gcaccagagt
     1501 ctatacctcg actcccaaga aacccaattc tgctttgcgt aaggtggcga gggtaaggtt
     1561 aactaatagt tttgaggtca cggcttacat accaggtgaa ggccataacc tgcaagaaca
     1621 ttccattgtt ttaattcgag ggggaagggt gaaggatttg ccgggagtcc gctatcacat
     1681 tattcgcggc accttagatg cgactggagt ggcggataga aagcagagta ggtcgaaata
     1741 cggaaccaaa aggccaaaga ttgaaaagga gtagcttaag tgtccagaaa gaaaggcttt
     1801 acaaaaagag agagattgcc agaccccaag tttaagagtg ttctggtgac tcaattcatc
     1861 aataacttaa tgcggcgagg aaaaaggagc ttggctgaga atgtattcta cgagactatg
     1921 gatttgatta ataagaagac ctcccagcca ggcttggacg ttttcaacaa agccttaaac
     1981 aacgttaagc cccttttgga agtgaaatcc agaagagtgg gaggagcgac ctatcagatc
     2041 ccgattgaag tgagagctga ccgtcgcacc gccttggcca tacgttgggt cataggttat
     2101 gccaaagaga gaagtgaaca taccatgcca gagaggttgg ccgctgaact agtagccgct
     2161 tcgaagaacg aagggacagc tataaagaag aaggaggata cacataagat ggcagaagcc
     2221 aacaaggctt ttgcgcattt tagatggtga ttgctacagg ttcacaaaag tatagactta
     2281 aaggtttgga aagattggta cggagcaagg ttctaaaaat ctccactttc gaagtggatg
     2341 agccgtctgc ttcccggatt tccacttttg atagatggag ttttttcatg gtagacagat
     2401 tccctttaga cagggtaaga aacataggca taatggcgca cgtcgatgca ggtaagacca
     2461 ctactacgga gagaatactt tattacactg gtaaaacgca tcgcataggt gaagtagatg
     2521 atggtgcagc tactatggat tggatggacc aggaaaaaga aaggggtatt accataactt
     2581 ccgctgccac gacctgtttt tggcgagatc atcagataaa catcatagat actccaggcc
     2641 atgttgattt cacagctgag gtggagcgct cgcttcgggt tctggacggt gctgttgctg
     2701 ttttctgtgg ggttggaggg gtcgagcctc agtccgaaac tgtgtggaga caagcggata
     2761 ggtatcgagt tccaaggata gcttttgtta acaagatgga tagggttgga gctgatttcg
     2821 aaatggtatt ggagacgatg ctggagagac tcaaggctag ggctgttcct atccaacttc
     2881 cggtcaaaaa gggagatgat ttcttgggca ttatagattt gattcagatg aattacaggg
     2941 tttatcatgc agacgatttg ggtaggactt tcgaagacag agagattcca aaagaatttg
     3001 aacagaagtc taagaagaga agagaggagc ttttggaggc gttgtcagat tacgatgagc
     3061 agttgcttga aaactttgtt cacgaaaaac caattgaaat tgagcagata aaaggggcct
     3121 tgagaaaagc caccttagat acgaagttag ttccagttct atgtggagct gctttgaaga
     3181 atcaaggtgt gcaaaaacta ttggatgcgg tgattgactt tttgccatct cctttggata
     3241 tgcctccggt gaaagggata aatcccaaga acgatcaaga aacccagaga aaagtagcgg
     3301 ataacgaata tttctgcgcc ttggctttca aggttgcaac cgacccctat gttggcaaag
     3361 taatctattt cagagtttac tccggaaaag tctcagctgg ctcacaggtt ttgaacagta
     3421 acacccagca gaaggaaagg atagttcgca tcttagaaat gcacgcagac aaaagggaag
     3481 aaaaaggcga agttcataca ggagatatcg cagccgtagt gggattaagg aacaccacta
     3541 ccggcaatac cttgtgcgac cccaagaagc cgataatctt agagaaaatg agttttcctg
     3601 aaccggtcat atcagtggca attgagccca aaaccaaggc ggatcaggat aaactggata
     3661 gcgctttatc gaaattgatg gatgaggacc caaccttcaa ggttaatttt aatgaagaga
     3721 ccgggcaaac gatcatctcc gggatgggtg aattacattt ggagatcttg gtggagagga
     3781 tgttgaggga gttcggagtt ggtgccaatg tgggcagacc acaggtgagc tacaaggaaa
     3841 gcataaccca gacggttgag tccgaaggga ggtttgtaaa gcagaccgga ggtaggggcc
     3901 attacgcttc cgtgaagata agactggaac ccacggagag gcataaaggt cttgtttttg
     3961 aaaataagct aaaaggtggc actattccca aggagttcgt tccagccata gagaaaggga
     4021 ttaaggaatc gatggaaagc ggtgtgatgg ctggttatcc ggtgatagat attaaagtta
     4081 ctcttctgga tgggagtttt cacgaggttg attcatctga aattgatttc aaggcagctg
     4141 cacgagatgc ccttgtgaat ggttttacga aagctaatcc aatactctta gagccggtga
     4201 tgaaggtgga agtggtgctt cccgaagagt atatgggtga agtcatagca gatttgaatg
     4261 ctcgtagagg aagagtggag agaacctcca gaagagctga tggatgggtt gtagatgcca
     4321 tggttccttt ggcggagatg tttggatatg ctactatctt gcgttcgctc tcgcaaggga
     4381 gggcaattta tactatggag ttttcacatt acgaagaggt tccagaagct atctctgaga
     4441 gcctgttgac taaaatcaga ggatattaaa aaggaaatag caggaggttt tatgtccaag
     4501 aagcgttttg agcgaaccaa gccccatctt aacattggta ccatcggaca tgtggatcat
     4561 ggtaagacca cgttgactgc agctataacc atggtgttgc atcgtaagaa tatggcagac
//
LOCUS       DASWLN010000049         3855 bp    DNA     linear   ENV 20-FEB-2024
DEFINITION  MAG TPA_asm: Candidatus Zixiibacteriota bacterium isolate
            SMAG_U2949 k141_49367, whole genome shotgun sequence.
ACCESSION   DASWLN010000049 DASWLN010000000
VERSION     DASWLN010000049.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37960594
            Sequence Read Archive: SRR10376091
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Candidatus Zixiibacteriota bacterium (soil metagenome)
  ORGANISM  Candidatus Zixiibacteriota bacterium
            Bacteria.
REFERENCE   1  (bases 1 to 3855)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 3855)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (26-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/12/2024 08:28:26
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 1,440
            CDSs (total)                      :: 1,416
            Genes (coding)                    :: 1,412
            CDSs (with protein)               :: 1,412
            Genes (RNA)                       :: 24
            rRNAs                             :: 1 (16S)
            partial rRNAs                     :: 1 (16S)
            tRNAs                             :: 21
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 4
            CDSs (without protein)            :: 4
            Pseudo Genes (ambiguous residues) :: 0 of 4
            Pseudo Genes (frameshifted)       :: 0 of 4
            Pseudo Genes (incomplete)         :: 3 of 4
            Pseudo Genes (internal stop)      :: 1 of 4
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..3855
                     /organism="Candidatus Zixiibacteriota bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_49367"
                     /isolate="SMAG_U2949"
                     /isolation_source="soil"
                     /db_xref="taxon:2053527"
                     /environmental_sample
                     /country="USA: Oregon"
                     /lat_lon="42.99 N 123.63 W"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            complement(110..541)
                     /locus_tag="VGB16_01900"
     CDS             complement(110..541)
                     /locus_tag="VGB16_01900"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_013075795.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0120225 - coenzyme A binding [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="CoA-binding protein"
                     /protein_id="HEX9916466.1"
                     /translation="MPEEKKYLNPSSKGIDEILDSNKIVAVVGLSSNSARPSYCVADY
                     LKSKGYTIIPVNPKEKEVLGEKAYPTLKEIPQKVDIVDIFRQPELVLPIIDDAIAVGA
                     KVIWMQEGVINHQAALKASKHGLKVVMDRCILKEVRRRLNR"
     gene            complement(545..877)
                     /gene="trxA"
                     /locus_tag="VGB16_01905"
     CDS             complement(545..877)
                     /gene="trxA"
                     /locus_tag="VGB16_01905"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_012842698.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0005737 - cytoplasm [Evidence IEA];
                     GO_function: GO:0015036 - disulfide oxidoreductase
                     activity [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="thioredoxin"
                     /protein_id="HEX9916467.1"
                     /translation="MNDTIEFTDDNFEVEVLKSDKPVLVDFWASWCGPCRMVGPIVEE
                     IGKEYEGKLKVGKLNVDDHNRTASQYGIMSIPSLLFFKAGQVVDQVVGAVPKQTLTDK
                     IDKVLGKT"
     gene            complement(874..1905)
                     /gene="lpxD"
                     /locus_tag="VGB16_01910"
     CDS             complement(874..1905)
                     /gene="lpxD"
                     /locus_tag="VGB16_01910"
                     /EC_number="2.3.1.191"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_009059108.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016410 - N-acyltransferase activity
                     [Evidence IEA];
                     GO_process: GO:0009245 - lipid A biosynthetic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="UDP-3-O-(3-hydroxymyristoyl)glucosamine
                     N-acyltransferase"
                     /protein_id="HEX9916468.1"
                     /translation="MKTLGEIATYLGGKVSGDESLKIKRIYGIEQAQPGDLTFVANPK
                     YAGFLSTTKASAAIVGEDVKTDRLSLILHPHPYFAFCRALGLFHPQTKKYEPGIHPTV
                     SLSKKSKIAKNAHIGPYVVIEDGVDIKSGVTILAGSFIGARTSIGQNSFIYPQVIIRE
                     DTIIGKNVIIHPGTVIGSDGFGYVKNKGVHHKILQVGKVVIEDEVEIGANVTIDRATL
                     GETRIGKGTKIDNLVQIAHNVVIGENTLIAAQVGISGSTKIGKDVVIAGQVGLVGHIE
                     IGDRVIIGAQSGVSKSVKSDTFIFGYPAREIHRSMRIEACISQLPEYFKRITQLEKKI
                     RGGAKKEDR"
     gene            complement(1906..2436)
                     /locus_tag="VGB16_01915"
     CDS             complement(1906..2436)
                     /locus_tag="VGB16_01915"
                     /inference="COORDINATES: protein motif:HMM:NF015871.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="OmpH family outer membrane protein"
                     /protein_id="HEX9916469.1"
                     /translation="MKILKILLLSLIFLVCFCALAFPEAGKFGFVNSLRIQTEYKEFA
                     DAQDKLDKDVTMWQTELDSMKTEIDSLQYELDKQSLLLSEEKKKDKEDVIRGKKLKYK
                     QYSTEVFGVNGKMERRNAELVKPIRDKIMLIIEKIAREGNYSFIFDSVGSNIAYADRK
                     LDLTDKVLEELSKLEK"
     gene            complement(2451..>3855)
                     /gene="bamA"
                     /locus_tag="VGB16_01920"
     CDS             complement(2451..>3855)
                     /gene="bamA"
                     /locus_tag="VGB16_01920"
                     /inference="COORDINATES: protein motif:HMM:TIGR03303.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0009279 - cell outer membrane [Evidence
                     IEA]"
                     /codon_start=2
                     /transl_table=11
                     /product="outer membrane protein assembly factor BamA"
                     /protein_id="HEX9916470.1"
                     /translation="IYKQEKYDQTLADIYALYHEEGYLYARVEDNTATQDSTLNVNYN
                     INEGIPANVRKINIEGNTKTKEKVIRRELAIMPGQRFRRSSLMRSLRNMNYLNYFSNV
                     IPDYQVLENGDVDLIIKVEEKPTGSIQFGAGYSQTDKLVGTLGLGIPNLFGNGQNLEL
                     NLDFGKRRNTIQASFTEPWFMDTPTSVGLDIYQINKNWYDDFTEQRIGGSLGLGRRLK
                     WPDSYFRVAWRYRLEDQKYYDFDSTYLAEVENKSYDLSQTNWPLRNSSTNFSIIRDSR
                     DLSQFATRGSVLSWNTELAGGVLGGDYDYHKHIFEASKYVKTFWSFVLAMRMKVGVVD
                     GYKSAEVPYSERFSPGGTDPDGVIRGYNDSSIGPRDEDGYYLRGRSVLVYNAEYQFPV
                     VEQQIYGLFFFDMGNSWLAGKDIAPFDFKGLYKSAGFGLRLVLPPLGMIGFDMGYGFD
                     KERGGVWRPHFQIGSTF"
BASE COUNT          972 a          996 c          663 g         1224 t
ORIGIN      
        1 gctttgccca tacgatttaa ctaaagaatc cattataacc gaatgccccg attcggtctt
       61 tccctcgaaa cgcaaatctc ccttccagat tatcttggat agcataacct cacctattta
      121 ggcgcctgcg aacctcttta agtatacacc tatccattac tactttcaat ccatgtttcg
      181 atgctttaag ggctgcctga tgattgatta ctccttcctg catccagatg actttagctc
      241 ctactgcgat tgcatcatct ataatgggaa gcactaactc gggttgtcga aagatatcca
      301 ctatatccac tttttgaggt atctctttta aggtagggta agctttttct cccaagacct
      361 ctttttcttt ggggttaacc gggattatcg tatagccctt gcttttgaga taatcagcca
      421 cgcaatatga gggacgagct gaattagaag acaagccgac taccgcaact attttgttgc
      481 tatctaggat ctcatctatt cctttagatg aaggatttaa atactttttt tcctctggca
      541 tattttatgt tttacccaac actttatcta tcttatcggt tagcgtttgc ttgggaactg
      601 ctcccaccac ctgatcaacc acctgcccgg ctttgaagaa aagcaaggat ggaatgctca
      661 taattccata ctgggatgcg gtcctattgt ggtcatctac gttcagcttc ccgactttta
      721 gtttaccttc atattcttta cctatctcct ccactatagg acccaccatt ctgcaaggcc
      781 cacaccaaga cgcccagaaa tccaccaaaa ctggcttatc cgattttaag acctcaactt
      841 caaaattgtc atctgtaaac tcaattgtgt cgttcatctg tcctcctttt tggctccacc
      901 tctaatcttc ttctctaatt gggttatacg tttgaaatac tccggcagtt gggaaataca
      961 agcttctatc ctcatagatc tatgtatttc cctggcagga tatccaaaga tgaacgtatc
     1021 gcttttaacc gacttactca ctcctgactg cgctccaata atgactcgat ctccgatctc
     1081 aatatgtccc accaatccca cctgacccgc aattactaca tcctttccta tctttgtgct
     1141 gcctgatatc cccacctgag cggctatcag ggtgttttct ccgattacca cattgtgggc
     1201 aatctgcacc agattatcta ttttggttcc tttcccgata cgggtttccc ccaaagtggc
     1261 tcgatcaata gtcacatttg cacctatctc cacctcgtct tcgatcacca cttttcctac
     1321 ctgcagaatc ttatgatgaa ctcccttatt cttaacatac ccaaacccat cacttcctat
     1381 gactgtgcct ggatgaatga taacgttttt tcctatgatt gtatcttctc ttattattac
     1441 ctgaggatag ataaaagaat tctgtccaat tgatgttctg gcgccgatga agctgccagc
     1501 caatattgtg accccgctct ttatatccac tccatcctct atcaccacgt aaggcccaat
     1561 atgggcattt tttgctattt tacttttctt tgacaaacta acagttggat gaatacctgg
     1621 ctcgtatttt ttggtttggg gatggaacaa tcctagggct ctgcagaaag caaaataagg
     1681 gtgaggatgc agtatcaggg aaagtctgtc cgtttttaca tcttccccca ctatggcagc
     1741 tgaagctttg gtggtggaaa gaaaacctgc atatttggga ttagctacaa aggtcagatc
     1801 acctggctgg gcttgctcaa ttccgtatat tctttttatc ttgagggatt catccccgct
     1861 caccttgcca cccagatagg tggcgatttc acccaaagtc ttcatctatt tttctaactt
     1921 gcttagctcc tctaacacct tatctgtcag gtctaatttc ctatcagcat aggcgatgtt
     1981 gcttcctact gagtcgaaaa tgaaactata gtttccttcc cgggctatct tctctataat
     2041 caacattatc ttatcacgga tcggcttaac taattccgca ttccttcttt ccatcttccc
     2101 gttgactccg aagacttcag tggaatattg cttatatttc agtttcttgc cccttatgac
     2161 atcctctttg tcttttttct tctcttcact caagagcaag ctctgtttgt ctaactcata
     2221 ctgcaggctg tctatttccg ttttcatcga atccagttca gtctgccaca tagtcacatc
     2281 tttatccagc ttgtcttggg catccgcgaa ctctttatat tctgtttgaa tgcgaagcga
     2341 gttgacaaat ccgaacttac cggcttcagg gaaagctaag gcacaaaaac aaacaagaaa
     2401 gattaaagat aagagtaata tcttcagaat cttcatctgc tcccccgttt ttagaaggtg
     2461 ctacctattt gaaaatgcgg cctccacact cctcccctct ccttatcaaa cccatatccc
     2521 atatcaaatc caatcattcc taaaggaggc aacactaatc tcaacccaaa accagcagac
     2581 ttatagagac ctttaaaatc aaagggtgct atatcctttc cagccaacca gctgtttccc
     2641 atatcgaaaa agaaaagtcc gtatatctgc tgctccacaa cgggaaattg gtattcagcg
     2701 ttgtaaacca gaacacttct gcctctcaaa taatagccgt cttcatccct gggacctatg
     2761 gagctgtcgt tataccccct tattacacca tctgggtcgg tcccacccgg cgaaaacctc
     2821 tcgctgtatg gaacctcagc gcttttgtac ccatccacta cccccacttt cattctcatt
     2881 gccaacacga aactccagaa ggtctttaca tacttggaag cttcaaatat gtgtttgtgg
     2941 taatcgtaat ctccacccaa gaccccaccc gctaattcgg tgttccacga aagaactgaa
     3001 cctctggtgg cgaattgaga taagtccctg ctgtcgcgga taatggagaa atttgtgctg
     3061 gagttcctca aaggccagtt tgtttgactt aaatcataag acttattttc tacttcggct
     3121 aagtaagttg agtcgaaatc ataatacttt tggtcttcca aacgataacg ccaggcaact
     3181 ctgaagtagc tatccggcca cttcaatctc ctacccaatc ccaaacttcc acctattctt
     3241 tgttcggtga aatcatcata ccaattcttg tttatctgat atatgtctag accgactgaa
     3301 gtaggggtat ccataaacca gggctccgtg aagctggctt gaatggtgtt tctccttttc
     3361 ccgaaatcta agttcaattc tagattctgc ccattcccaa aaagattcgg gatgcccaat
     3421 cccaaagtcc ccactaactt atccgtctgg ctataccctg ctccaaattg aatactgccg
     3481 gttggctttt cctcaacttt gataatcaaa tcaacatctc cattttccag aacctgataa
     3541 tctggtatga cgttggaaaa gtaattaagg taattcatgt tgcgcaagct cctcattaag
     3601 cttgagcgtc tgaatctctg accaggcatt atggctaatt cccggcggat aaccttttcc
     3661 ttggttttgg tattaccctc tatatttatc tttctgacat tggctggaat cccctcgttg
     3721 atattgtagt tgacattcaa ggtgctgtcc tgagtggcgg tattatcctc tactcgcgcg
     3781 taaagataac cttcttcatg ataaagagca tagatgtcag ccagggtttg gtcatatttt
     3841 tcttgcttgt agatt
//
LOCUS       DASWLN010000050         6410 bp    DNA     linear   ENV 20-FEB-2024
DEFINITION  MAG TPA_asm: Candidatus Zixiibacteriota bacterium isolate
            SMAG_U2949 k141_434013, whole genome shotgun sequence.
ACCESSION   DASWLN010000050 DASWLN010000000
VERSION     DASWLN010000050.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37960594
            Sequence Read Archive: SRR10376091
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Candidatus Zixiibacteriota bacterium (soil metagenome)
  ORGANISM  Candidatus Zixiibacteriota bacterium
            Bacteria.
REFERENCE   1  (bases 1 to 6410)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 6410)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (26-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/12/2024 08:28:26
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 1,440
            CDSs (total)                      :: 1,416
            Genes (coding)                    :: 1,412
            CDSs (with protein)               :: 1,412
            Genes (RNA)                       :: 24
            rRNAs                             :: 1 (16S)
            partial rRNAs                     :: 1 (16S)
            tRNAs                             :: 21
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 4
            CDSs (without protein)            :: 4
            Pseudo Genes (ambiguous residues) :: 0 of 4
            Pseudo Genes (frameshifted)       :: 0 of 4
            Pseudo Genes (incomplete)         :: 3 of 4
            Pseudo Genes (internal stop)      :: 1 of 4
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..6410
                     /organism="Candidatus Zixiibacteriota bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_434013"
                     /isolate="SMAG_U2949"
                     /isolation_source="soil"
                     /db_xref="taxon:2053527"
                     /environmental_sample
                     /country="USA: Oregon"
                     /lat_lon="42.99 N 123.63 W"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            complement(<1..1432)
                     /locus_tag="VGB16_01925"
     CDS             complement(<1..1432)
                     /locus_tag="VGB16_01925"
                     /inference="COORDINATES: protein motif:HMM:NF013283.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0019867 - outer membrane [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="BamA/TamA family outer membrane protein"
                     /protein_id="HEX9916471.1"
                     /translation="MIKIRLLLLILIILLSQGRLRAQTEIKNIRQKPVVKDMIFSGNT
                     YFSNNKIADQMSTQKNRFYNFLKRKKLNPRRLSMDALAIDSLYHTNGFLEAKASIDYK
                     IENGKAIVEVKIDEGKQTKLGKLEGTGGLKDLNAAVEKIIDKAEKGKPYNPVEIDDIC
                     FGIRTVYANHGYPYVKIEKQIERVEEKNVIDVSLKIFPDEKVFFGEVTCQGLKFTHPQ
                     VALREIVFKPGETYSREKLIYSEQRIYSTGLFNYITLDAKELEAKPLKADFNLRLVEK
                     KPSFVRFNAGLARDQQQDLSLDFLGEWGNRNLFGTGRNLSLSAYTSWVIITNLKNLNN
                     RFAINYTEPWFVGIRLPLFLDVYYEPGVRSVFQEDYRIESWGGDITFVKEFYRYTKVW
                     LGGGYQRVYISGVPQDQQERFLNEIGINVRRKINLSAERDTRDNPLIPSKGAFTQVNT
                     EQVGGFLGGQNNLIKFNISWNRYNPLG"
     gene            complement(1433..4978)
                     /locus_tag="VGB16_01930"
     CDS             complement(1433..4978)
                     /locus_tag="VGB16_01930"
                     /inference="COORDINATES: protein motif:HMM:NF016260.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0005886 - plasma membrane [Evidence IEA];
                     GO_process: GO:0009306 - protein secretion [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="translocation/assembly module TamB
                     domain-containing protein"
                     /protein_id="HEX9916472.1"
                     /translation="MRKRFKIPLLTILIVICLILLCILILANTKILENRASYTINTVF
                     LKKYPLKVKIGDIGGDILNNISLRNLSIDYIKPNEAYRIVQIDEVRAKYNLFNLLGRR
                     WKLDLLEIYDPQIDLSKFEQPKPLSASQQHLPNFKIDDIRIIGGEIRFLSGGKEEKIS
                     ILNLRLALNHQEDNTEVDILQGGASSFSRKISIKTLEGKLNYSQDILDVANFHLVTPR
                     LDIGLKGKIVGFQKPTFSFELDIPKLDFEELNRIVGTKLSGNISAKGTCSGGLHKFKG
                     EMQLDGKFFEKDFDGVGARYLYADKILTLEEISGKVFKSPIYGNGLIDFNITPQEYQL
                     KARIKNLDINRIVPTTTFHTDLSGEVSLFGQSFSEDEFIMDIQADLGRSRIDLYSFDQ
                     LQGGFSVTASDIFFFRGFMGRYKNTRFVFNGDIEYNNLMNVESEIYFNDLVDFENQTF
                     LGKIKGQGKANLEVFGKTLDFNVKGDFISDSAYVYDLFSRDVKIDFGVNNYIAGKNGQ
                     VDIQLLNGMGWGIPYDSIYGHLTLKGENLVIIDSAYFRNEYASASASGNYNQSFSTPQ
                     LFFDQFKLDYRGNKIELSQPLMMDIDKNLVRVGRADFNADGGGFWLSGKIQADSLNLE
                     AGFEDIDIDPWFSIFLPQKKVEGRLTGELDANGTLEKPQMKFEGKIGQARFDKLDWGD
                     LSAYLEYKDTSLNINALKLSSPDGIYTFSGFMPIDLSLKYVNQRFPDKPQKIDARFEG
                     KKINILPYFVPDLEYLKGDFKGELVMTGTPLSPSFDGQMSIKNGILKLEKLMDPVENL
                     ETDMIMQGTDIDFAKIKGIVKHEVGSSDNWWKRLWHRASYRKPKYGTVDVSGKLNVKE
                     INRFEYDFVFKGKDLPIRYEYADLSAVVDADLEVKGISPPLVSGDINISELYYKEPFG
                     SSEGQLGGIGDSSLWDLNLDMSADGQIFIMNYDMNAEFKGEVVISREKGKYTLLGEME
                     VIRGKYFLARTFNIDEGKMTFDNIEKIDPKLDFLLSTTVYAGDTLSFSEYNDIQLQVG
                     GTLLSPEVNPASGSPYSRGDIIQLLAVGYSPSAEDTSRTQTQLQNRLLNSLEGTIGAL
                     GSRALENWATNTIGLETFEIRPEQKGRFSLWSPEVTVGKYFANRFYVRYTFGPSQGQE
                     NHWSLEYRLSKHFLLEASRDREDKFHLGFNLSWDY"
     gene            complement(5279..>6410)
                     /locus_tag="VGB16_01935"
     CDS             complement(5279..>6410)
                     /locus_tag="VGB16_01935"
                     /inference="COORDINATES: protein motif:HMM:NF024758.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=2
                     /transl_table=11
                     /product="trypsin-like peptidase domain-containing
                     protein"
                     /protein_id="HEX9916473.1"
                     /translation="KRTTPLPPGSYKGTFRKEEIMGAAPILIGSGVIYDKDGHILTTT
                     SVIGGMESFTVTLPDGKKVEGKLVGVDPEYNLAVLKIDAPGLVPAKLGYSDKLKVGSW
                     LTIVGNSYGIPLAVALGITSGQRKDGFIQMSANVSPGNSGGPVLNTKGEVVGLVSAKI
                     TEPSYISSIEIFRSESEGFSISPGQLDLPTSGVSLAIPINEAKIRADEIIKNGSIKRG
                     YLGVYITDLTEEQLRDHKIESGVMIDRVEEDSPAQEGGLSDGDIILSYDGKKVQDSGE
                     LSKMIKQTKPKIQVQLEALRDNKKKVIKVTLGESESRYAEGFGESYYEALNRFGQAMG
                     KLDREYNKGLKGSRADLQKKIDRLEKELQELREKLENTETND"
BASE COUNT         1700 a         1560 c         1160 g         1990 t
ORIGIN      
        1 ggcccagtgg attatatctg ttccaggaaa tattgaattt aatcaagttg ttttgccctc
       61 ccaaaaaacc gcccacctgc tcagtattaa cctgggtgaa tgctcccttt gaaggaatca
      121 aagggttatc ccgcgtatct ctctccgcag atagatttat cttccttctc acatttatcc
      181 ctatttcgtt gagaaatctt tcctgctggt cttgtggcac gccagagata tacacccttt
      241 gatatccccc ccccagccag actttggtgt atctatagaa ttctttaacg aaggtaatat
      301 ctcctcccca ggactcgatt ctgtaatcct cctgaaaaac actcctcact cccggctcat
      361 aataaacatc caaaaacaag ggtagtctta ttcctacaaa ccaaggctcg gtgtagttga
      421 tggcaaaacg gttattgagg ttttttaggt tggtgatgat gacccagctg gtgtaagctg
      481 acaagcttaa attgcggccc gttccaaaaa gatttctgtt tccccactct cccaaaaagt
      541 ccagactcaa atcctgctgc tggtcccgtg ctaaacccgc attaaacctg acgaaacttg
      601 gtttcttttc caccaaacgt agattgaaat cggccttgag aggcttggct tcaagctctt
      661 tcgcatctaa ggtgatatag ttaaagagtc cagtactata aatcctctgc tcgctataaa
      721 tgagtttctc tcgactataa gtttcacccg gcttgaaaac tatctccctt aatgctacct
      781 ggggatgagt aaactttaac ccctgacagg taacctcccc gaagaaaacc ttctcatccg
      841 gaaaaatctt caaactgaca tctattacat ttttctcttc aaccctctcg atctgtttct
      901 ctatcttaac ataaggataa ccatgattgg catatacagt gcgtatccca aaacagatgt
      961 catctatctc tacagggtta taaggtttcc ccttttctgc tttgtctatt attttctcca
     1021 cagcggcatt caaatccttt agtccaccag taccctccag cttacccagc ttggtctgtt
     1081 ttccctcatc gattttcacc tcaactatag cttttccatt ttctattttg taatctatac
     1141 tggctttggc ctccagaaaa ccattagtgt gataaagtga gtcgattgcc aaggcatcca
     1201 ttgacaacct gcgagggttg agcttctttc tcttgagaaa gttataaaac ctgttcttct
     1261 gggtagacat ctggtcggct atcttgttgt tggagaaata ggtgttgccg gagaagatca
     1321 tatctttaac cactggcttc tgcctgatgt tctttatttc ggtctgagcc cggagcctac
     1381 cttgacttaa aagaattatc aaaatcaaaa gaagaagtct tatcttaatc atttagtaat
     1441 cccagctgag attaaatcct aaatggaatt tatcttctcg gtcgcgagat gcctctaaga
     1501 gaaaatgttt gctcaaacga tactccagac tccagtgatt ctcctgaccc tgtgatgggc
     1561 cgaaagtata tctaacatag aaacggttgg caaaatattt cccaactgtg acttctggac
     1621 tccacaaact gaatcttcct ttttgttcgg gtcgtatctc gaatgtctcc aagccgatgg
     1681 tgttggtggc ccaattctct aaagccctgc tgcccaaagc tccaatcgtt ccctccaagc
     1741 tattcaaaag tctgttttga agttgagtct gtgttctgga tgtatcctca gcagaaggag
     1801 agtaccctac ggctaaaagc tgaatgatgt cgccccgcga ataaggtgag ccggaagcag
     1861 gattgacctc cggagacagg agcgtgccac cgacctgcaa ttgtatgtca ttatattcgc
     1921 tgaaggacaa ggtatcaccg gcataaaccg tggtggaaag gagaaagtcg agtttggggt
     1981 ctatcttctc tatattgtcg aaagtcattt tcccctcgtc tatgttaaaa gtcctggcta
     2041 agaaatattt ccctcttatt acctccatct ctcccagtaa ggtgtatttc cctttttccc
     2101 ttgaaatcac cacttctcct ttgaactccg cattcatatc ataattcatt atgaaaatct
     2161 gaccatctgc ggacatatcc aagtttagat cccacaaact actatcccct atgccaccca
     2221 gttgcccctc agaagagcca aaaggctctt tgtaataaag ttcggagatg tttatatctc
     2281 cagaaaccag aggcgggctt attcccttta cctccaaatc cgcatccacc acagctgaaa
     2341 ggtcagcata ctcatatctg ataggtaaat cttttccttt gaatacaaaa tcgtattcga
     2401 aacgattgat ttctttgaca ttcaattttc cactgacatc caccgtgccg tatttcggct
     2461 tacgataaga cgccctatgc cataatcttt tccaccagtt gtctgatgag ccaacttcgt
     2521 gcttaactat cccctttatc ttcgcaaaat caatatccgt tccctgcata atcatatctg
     2581 tctctaagtt ctcaaccggg tccatcagtt tttccagttt cagaattcca ttttttatgc
     2641 tcatctggcc atcgaagctg gggctcaaag gtgtgccggt catcaccaac tcacctttga
     2701 aatctccctt gagatattcc aggtcaggaa caaagtaagg caggatattt atctttttgc
     2761 cttcaaatcg ggcatctatt ttctgcggct tatccggaaa cctttgattc acatatttca
     2821 aggaaaggtc tatgggcata aaaccggaaa aggtgtaaat tccgtctggg ctggataatt
     2881 taagcgcatt gatgttaagc gaggtatcct tgtattccag gtaggcggaa aggtctcccc
     2941 aatccaactt gtcaaaccta gcttgtccta tttttccttc aaacttcatt tgcggttttt
     3001 ccagagtgcc gtttgcatcc agttctccag ttaatctccc ctctaccttc ttttggggca
     3061 gaaagatgga aaaccaagga tcaatatcga tatcctcaaa tcctgcttct aaattcagac
     3121 tatcggcttg tatctttcca gataaccaga atccgccgcc atctgcgttg aaatccgctc
     3181 ttcccactct aaccagattc ttgtctatat ccatcatcaa aggttgactt agttctattt
     3241 tattcccacg atagtccagc ttgaattggt caaagaacag ttgcggtgtg gaaaaggatt
     3301 gattgtaatt tccactggca gaagcagagg cgtattcatt tctgaaatag gcggagtcga
     3361 taatcaccaa gttttctcct ttaagggtca gatgaccata gatgctgtcg taaggaatac
     3421 cccaacccat accatttagc agctgaatat cgacttggcc attttttcca gcgatataat
     3481 tgttaacccc aaaatcgatt ttcacatctc tgctgaaaag gtcatagaca taagctgagt
     3541 ctgaaatgaa atctccctta acattaaaat ccaaagtctt gccaaatacc tctaagtttg
     3601 ctttgccctg tcctttaatc ttccccagaa aggtttggtt ttcaaaatca accagatcat
     3661 tgaaatagat ttcgctctct acattcatca ggttattgta ttctatgtcg ccgttaaaca
     3721 caaatcgtgt attcttgtac cttcccataa atccgcgaaa gaaaaatata tcactggctg
     3781 tcactgaaaa acctccctgc agttggtcaa aggaataaag gtctatcctg cttctgccta
     3841 aatctgcctg aatatccatt ataaattcat cctcggaaaa actctgaccg aaaagtgaaa
     3901 cttctccact caggtcagta tggaaagttg tggtcggaac gattcgattg atatccagat
     3961 tcttaattct ggctttaagc tggtactcct gcggggttat attgaaatct attagtccgt
     4021 ttccgtagat tggagatttg aacacctttc cgcttatctc ctccaaagtc aaaatcttat
     4081 cggcgtaaag gtatctggcg ccaacaccat caaaatcttt ttcaaagaat tttccatcca
     4141 attgcatctc acctttgaac ttgtgaagac caccagagca agtccctttg gcacttatgt
     4201 tacccgaaag ttttgtgccc acaattcgat ttaattcctc aaaatccaac ttcggtatat
     4261 ccaactcaaa agagaaggta ggtttctgaa aaccaactat ttttcctttc aggcctatgt
     4321 ctagccgcgg ggttaccaga tggaaattcg ctacatccaa gatgtcttgg ctatagttta
     4381 gtttaccctc cagggtcttg atggaaatct ttcttgaaaa ggaggatgcc ccgccttgaa
     4441 ggatgtcgac ttcggtatta tcctcttgat ggtttaaagc cagacgcaga ttcaaaatgc
     4501 ttatcttttc ctctttaccc ccagaaagga atcttatctc ccctccgatt atccggatat
     4561 catctatctt gaagtttggg agatgctgct gggaggcgga aagaggttta ggttgttcaa
     4621 acttgctcag gtctatctgg ggatcgtata tctctaacag gtccagcttc catcgcctgc
     4681 ctaatagatt aaaaagatta tacttggctc ttacctcatc aatctgaacg atacggtatg
     4741 cttcattcgg cttaatgtaa tctatgctta gatttcgaag ggaaatgtta tttagaatat
     4801 ctccgccaat atctccgatt ttgactttga gaggatattt tttcaggaag acggtgttta
     4861 tggtgtaact tgctcgattc tccaagattt tagtgttagc caggatgaga atgcaaagca
     4921 agataagaca aattacaatc aggatagtta acaatggaat cttgaatctt tttctcattt
     4981 gaccaaatta tatcaaaata tgagtaatag gtcaagtcaa aagcaacttc tcaagctcaa
     5041 aagttcaaaa gaccaccaag taactcagct gcacttatta tatcggcatt tctgtttttg
     5101 cacatattcc ccaaaaagca tgaattgggt agccccgtgg catccgccac ggggaatgcg
     5161 gttgtcttaa tttgtctata acaccctaat tatgcccaaa aataaagaat caccttccgc
     5221 caataatttc ttagaaggcg attcttcctc aagaaggagg tgttgccaat tacctctttc
     5281 agtcgttagt ctcggtgttt tctaacttct ctcttaattc ttgcaattct ttttctaatc
     5341 tatcaatctt cttctgtaaa tcagcgcgag accctttaag ccccttatta tattctctat
     5401 ccaatttgcc catcgcttgg ccaaaccgat tcagggcttc ataatacgac tctccaaaac
     5461 cttcggcata acgcgattca gattcaccca gagttacttt tatcactttt tttttgttgt
     5521 cacgcaatgc ctcaagctga acttggattt tgggtttggt ctgttttatc attttggaaa
     5581 gctcccctga atcctggacc tttttaccat cataagatag aattatatca ccatccgata
     5641 gtccaccctc ttgggctgga gaatcttctt ctaccctatc aatcatcact ccactttcta
     5701 ttttgtggtc cctcaactgc tcttcagtca gatcagtgat atagactccg agataacctc
     5761 ttttgatgct cccgttcttt attatctcat ccgccctaat cttagcctca tttatgggta
     5821 tggctaaact tactcctgag gtcggtaaat ccaattggcc cggggagata gagaagcctt
     5881 cggactcgga cctgaatatc tcgatactgc taatgtatga gggctctgtt atcttggctg
     5941 aaaccagacc caccacttcc cctttagtgt tcaaaaccgg gccgccacta ttccccggac
     6001 tgacattggc actcatctga atgaaaccat ctttcctttg cccacttgtt atccccaagg
     6061 caaccgccaa aggaatccca tacgaatttc ccacaatggt caaccatgag ccaactttga
     6121 gtttgtctga atatcccaat ttagccggaa ccagacccgg agcatcaatt ttcaaaactg
     6181 ccagattata ctccgggtca actcctacca actttccttc cacttttttt ccatccggaa
     6241 gggtgacggt gaaactttcc attccaccga ttacactggt ggtggtgagg atatggccgt
     6301 ctttgtcata tattacgcct gagcctatta gaatcggagc tgcacccatt atttcttctt
     6361 tcctgaaagt acctttataa ctgcctggtg gaaggggcgt agttctcttg
//
LOCUS       DASWLN010000051         3536 bp    DNA     linear   ENV 20-FEB-2024
DEFINITION  MAG TPA_asm: Candidatus Zixiibacteriota bacterium isolate
            SMAG_U2949 k141_559915, whole genome shotgun sequence.
ACCESSION   DASWLN010000051 DASWLN010000000
VERSION     DASWLN010000051.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37960594
            Sequence Read Archive: SRR10376091
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Candidatus Zixiibacteriota bacterium (soil metagenome)
  ORGANISM  Candidatus Zixiibacteriota bacterium
            Bacteria.
REFERENCE   1  (bases 1 to 3536)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 3536)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (26-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/12/2024 08:28:26
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 1,440
            CDSs (total)                      :: 1,416
            Genes (coding)                    :: 1,412
            CDSs (with protein)               :: 1,412
            Genes (RNA)                       :: 24
            rRNAs                             :: 1 (16S)
            partial rRNAs                     :: 1 (16S)
            tRNAs                             :: 21
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 4
            CDSs (without protein)            :: 4
            Pseudo Genes (ambiguous residues) :: 0 of 4
            Pseudo Genes (frameshifted)       :: 0 of 4
            Pseudo Genes (incomplete)         :: 3 of 4
            Pseudo Genes (internal stop)      :: 1 of 4
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..3536
                     /organism="Candidatus Zixiibacteriota bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_559915"
                     /isolate="SMAG_U2949"
                     /isolation_source="soil"
                     /db_xref="taxon:2053527"
                     /environmental_sample
                     /country="USA: Oregon"
                     /lat_lon="42.99 N 123.63 W"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            <1..181
                     /locus_tag="VGB16_01940"
     CDS             <1..181
                     /locus_tag="VGB16_01940"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=2
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEX9916474.1"
                     /translation="RSSKRSKSIFNLIIPGACHRIALCYSALDAESRKFVSFGFLSQD
                     TSQDPEFGEFALSQE"
     gene            244..1155
                     /locus_tag="VGB16_01945"
     CDS             244..1155
                     /locus_tag="VGB16_01945"
                     /inference="COORDINATES: protein motif:HMM:NF012515.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="PfkB family carbohydrate kinase"
                     /protein_id="HEX9916475.1"
                     /translation="MRKITVIGSITQDTINLPYGKQTKSFGGIFYNLLSLSYLSSKNV
                     KTYPICNLGEDIHKDVIKILDERENIVKGGIKKSKGRNNAVCLFCKKSGKRKEFLLSL
                     VPPLNFSQIKPYLNSALILVNFISGFDLSLKTLEKIRASTTKPIYIDIHSLSLGVKKN
                     GERFPKKPKLWKRYIKCADMLQMNWGEFSILSGRKVKNINQIKVWAEKILKLGPKVII
                     ITLGKRGAFMMYCKEDKTGYYFSQNRTDEVMDAVGCGDVFTSAFIISYLQTRNLKSSL
                     DFANKVANFKSTFSGAGNLRELSRFAR"
     gene            1894..1966
                     /locus_tag="VGB16_01950"
     tRNA            1894..1966
                     /locus_tag="VGB16_01950"
                     /product="tRNA-Thr"
                     /inference="COORDINATES: profile:tRNAscan-SE:2.0.12"
                     /note="Derived by automated computational analysis using
                     gene prediction method: tRNAscan-SE."
                     /anticodon=(pos:1927..1929,aa:Thr,seq:cgt)
     gene            2501..>3536
                     /locus_tag="VGB16_01955"
     rRNA            2501..>3536
                     /locus_tag="VGB16_01955"
                     /product="16S ribosomal RNA"
                     /inference="COORDINATES: nucleotide
                     motif:Rfam:14.4:RF00177"
                     /inference="COORDINATES: profile:INFERNAL:1.1.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: cmsearch."
                     /db_xref="RFAM:RF00177"
BASE COUNT         1046 a          665 c          826 g          999 t
ORIGIN      
        1 gaggtcttcc aagcggtcaa aaagtatctt taacttgata attcccggag cttgccatag
       61 gattgccttg tgttattccg cacttgatgc ggaatccaga aagtttgtat cttttggatt
      121 cctgtcccaa gatacatctc aagacccaga attcggggaa ttcgcacttt cgcaggaatg
      181 acatagtaag gtttccagca acgatattga ttttaggttc ctgtgcttgt cgcaggaggt
      241 tcattgcgca aaataacagt aatcggcagt atcactcaag atacaattaa cctaccctac
      301 ggaaagcaga ccaaaagctt cggcggaata ttttataacc ttctgtccct atcctatctt
      361 agttccaaaa atgtgaaaac atatccgatt tgtaatttag gagaggatat ccacaaagat
      421 gtgattaaga ttttagatga aagagaaaat atagtaaaag ggggcatcaa aaaaagtaaa
      481 gggaggaata atgcagtttg cttgttttgt aaaaagagcg gcaagagaaa ggagtttttg
      541 ttgagcttgg ttccaccttt aaatttctca caaattaagc catatttgaa ttcggcttta
      601 attttggtga atttcatttc gggatttgat ttgagtctga agactttaga gaagattcgt
      661 gcaagcacga caaaaccaat atatatcgat attcacagct taagtttggg agtaaagaaa
      721 aacggagagc ggttccccaa gaaaccaaag ctctggaaaa gatacataaa atgcgctgat
      781 atgttgcaga tgaattgggg ggagttttcc attttatctg gcagaaaagt taaaaacata
      841 aatcagataa aagtatgggc agagaagatt ctgaaactag gaccaaaagt tattataatc
      901 actttgggca aaaggggagc attcatgatg tattgcaaag aggataaaac aggatattat
      961 ttttcccaaa atagaacaga cgaagttatg gatgcagttg gatgtggtga tgtcttcacc
     1021 tccgccttca tcatctctta ccttcaaacc agaaatctta aatccagtct ggatttcgcc
     1081 aataaagtcg ccaactttaa atctaccttt tcaggtgccg gtaacttgag agaactttcc
     1141 agatttgccc ggtaattttt caagtcagca aaaagccctc cagctatatg ctcgagggct
     1201 ctttaatctt atccataaat ctaaagagct attttttctt cttctttgct gtctttttgg
     1261 tctttggata tttcttgctg caaccgcagg tattacaaac cattatcttc acctccttat
     1321 cttatattag aagattcatt caaactataa gcgtattaaa catttttgtc aatagaaaag
     1381 gattgtggat ttcatcttat cgaacttaag ggagtacaga tgatatttta tagtattgtc
     1441 tctaagagac gatggcgaaa aataatcccg ccaaaggcgg gattattcac attatctata
     1501 aactttgtca attatttttt cttcttcttc ttagatggct tcttagtatg catctttccg
     1561 caggccattt tccctttctc acctcctcaa aaggttagaa gatttaccca gtgaaatata
     1621 tattaacaca aaattatttg tcaagacaaa gttgtggtct tgcaagattt tttcatttga
     1681 tgttggatga aattttggtt tcgaatttga cttgactttg agagggtgat atatagattt
     1741 atgtttggtt tcggtcgggc ggagatacgg ttcgacaggc tcaccgtcct gagcaaagtc
     1801 gaaggaaact ccgcccctac gcgagcggat aacaatttag gattatttta aatttaaact
     1861 tgtaaactgt taaactttta aactgaatta gtcgccaccg tagctcagtt ggtagagcag
     1921 gggtttcgta aacctcaggt cacccgttca agccgggtcg gtggctttca agacagttta
     1981 aaagtttaga agtttatgat gtgtaggggc aggcccctgt gcctgccctg aataatacaa
     2041 ttttagaacc tgccttaagc taaaaccagg gcggtcgtgg gagcccgccc ctacgaatag
     2101 aggaagattt gtagtaatct ttggttggtt cgggaattgt attcttcgag ccaattttgt
     2161 aggggttgga gaaatcctgc ccctacatca cataataaaa aaatattaaa aaagacttga
     2221 caagatatgg ctaaagtatt atactccgca agacgaaaat aggtaaggag aactccacct
     2281 atctttagtc aataattaaa cttaagagag tttcgtgaaa gagtttaatc cctggtagat
     2341 tttgcttgac aaatgctgga ttttgtttat attaaaaatc tgccatcagc atttgttggc
     2401 agattttttt aacctattgt tctttgaaaa ttaaatagcg tgcataagga gaagatgttg
     2461 tgagtttttt tgcgatccag atttcgaaac aatcaatata tatttggaga gtttgatcct
     2521 ggctcagaac gaacgctggc ggcgtgccta atacatgcaa gtcgtacgag aaagtccccg
     2581 caaggggata agtaaagtgg cggactggtg agtaacacgt gggtaacctg ccttaaagat
     2641 gggaataagc tttcgaaagg aagtctaata cccagtaatg ttccgaaaag gcatcttttc
     2701 ggagttaaag gtacctacct tcgggtagaa tatcgcttta agatgggccc gcgctctatt
     2761 agctagttgg tgagataaca gcccaccaag gctatgatgg atagccggcc tgagagggtg
     2821 tacggccaca ctgggactga gatacggccc agactcctac gggaggcagc agtagggaat
     2881 attgcgcaat gcccaaatgg gtgacgcagc aacgccgcgt ggaggatgaa gttcttcgga
     2941 atgtaaactc ctgtcagagg ggaagaatgc cccgatttat cggggttgac agtaccttca
     3001 gaggaagctc cggccaactc cgtgccagca gccgcggtaa tacgggggga gctagcgttg
     3061 ttcggaatca ctgggcgtaa agggtgtgta ggcgggtttg taagtcggat gtgaaaaccc
     3121 cgagcttaac tcggggactg catctgaaac tactagtctt gagtacggga gaggaaagtg
     3181 gaattcctgg tgtagcggtg acatgcgtag atatcaggaa gaacaccggt ggcgtaggcg
     3241 gctttccatt ccgatactga cgctgaaaca cgaaggctag gggagcaaac agaattagat
     3301 actctggtag tcctagctgt aaacgttgga cactaggtgt tggaagcgtc gaccctttca
     3361 gtaccgaagt taacacatta agtgtcccgc ctggggagta cggtcgcaag gctgaaactc
     3421 aaaggaattg acgggggccc gcacaagcgg tggagtatgt ggttcaattc gatgcaacgc
     3481 gcagaacctt acctgggtta gacaacactg gacatccctg gaaacagggc ttcccc
//
LOCUS       DASWLN010000052         7389 bp    DNA     linear   ENV 20-FEB-2024
DEFINITION  MAG TPA_asm: Candidatus Zixiibacteriota bacterium isolate
            SMAG_U2949 k141_51722, whole genome shotgun sequence.
ACCESSION   DASWLN010000052 DASWLN010000000
VERSION     DASWLN010000052.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37960594
            Sequence Read Archive: SRR10376091
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Candidatus Zixiibacteriota bacterium (soil metagenome)
  ORGANISM  Candidatus Zixiibacteriota bacterium
            Bacteria.
REFERENCE   1  (bases 1 to 7389)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 7389)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (26-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/12/2024 08:28:26
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 1,440
            CDSs (total)                      :: 1,416
            Genes (coding)                    :: 1,412
            CDSs (with protein)               :: 1,412
            Genes (RNA)                       :: 24
            rRNAs                             :: 1 (16S)
            partial rRNAs                     :: 1 (16S)
            tRNAs                             :: 21
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 4
            CDSs (without protein)            :: 4
            Pseudo Genes (ambiguous residues) :: 0 of 4
            Pseudo Genes (frameshifted)       :: 0 of 4
            Pseudo Genes (incomplete)         :: 3 of 4
            Pseudo Genes (internal stop)      :: 1 of 4
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..7389
                     /organism="Candidatus Zixiibacteriota bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_51722"
                     /isolate="SMAG_U2949"
                     /isolation_source="soil"
                     /db_xref="taxon:2053527"
                     /environmental_sample
                     /country="USA: Oregon"
                     /lat_lon="42.99 N 123.63 W"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            complement(<1..236)
                     /locus_tag="VGB16_01960"
     CDS             complement(<1..236)
                     /locus_tag="VGB16_01960"
                     /inference="COORDINATES: protein motif:HMM:NF012298.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEX9916476.1"
                     /translation="MSERVFDNRFCLLENLGHGRLGEVDKAYNIWNKQEVALRISYPE
                     ADSKAAEQGLKTEFVLLKELVHPGIVEVYDFGYG"
     gene            complement(304..2070)
                     /locus_tag="VGB16_01965"
     CDS             complement(304..2070)
                     /locus_tag="VGB16_01965"
                     /inference="COORDINATES: protein motif:HMM:NF024883.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ATP-binding protein"
                     /protein_id="HEX9916477.1"
                     /translation="MAKRKFAGKRDDKKDKPHDILTEMEDILHKVETAQPRLKNSNNS
                     EQGKLRSDDLEALLDISKAINSTLVLDDILQVVMKRAIKLLRAERGFLMLLDNENQLQ
                     YKTAYNICKESLSSDDFRISMSIANTVAKTGESIYTSDAQQDERFSRQKSVRELDLKS
                     IMCVPLKSKERIVGILYLDNSSKTNIFLKSDLYVFELFAGQAAIAIENAKLYESLLAM
                     KIYNENVVNRTPIGIIVVDNELKITIANRASQEIFRRAGWKDGSGEFGFANLSILDLL
                     PSKEKENWKRICYQVLYTGQPFEESRYYHKFETEELALSLKISPLNSLDNRIMGLIIV
                     IEDITEKVILEKYLILSEKLVAKGEMAASIGHELNNYLTIILNNAELLPINLKKGDLG
                     KVHQNTKSIVESVEMMKRFTDGLMDFSKLEAKLVEYDVKNLIEDLLFSVKPQKQFSNI
                     KFITNFDTQVPRLLIDVGQIQQVLLNLLNNAADAINKQEKSDGIVTISTHYRPESGVI
                     QIRVKDNGPGIPEELIDRIFEPRFTTKKDGHGFGLVTCEKIVKNHGGSVSTDSIPGRG
                     ATFTIELPVKKEQKITDKKLVA"
     gene            complement(3372..4046)
                     /locus_tag="VGB16_01970"
     CDS             complement(3372..4046)
                     /locus_tag="VGB16_01970"
                     /inference="COORDINATES: protein motif:HMM:NF012514.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="NUDIX hydrolase"
                     /protein_id="HEX9916478.1"
                     /translation="MKKTAKHKSRRRTWSINPVTRIKPSGKIYQRGKLKKTRNTEENE
                     YQRPCLRTGRNLRPIYRYCPACTSELVEKKADGKIRKCCPVCGFVLYRNPAPASGVII
                     EKDGKVLLVKRKFPPFKGDWSFPAGFMEYGESPEACAIREIYEETNLKIRLSGLFGVY
                     WGHDDPRTHAILVVYLADIIEGEPKPGDDAQEISFFSQDEIPTNIAFAAHRQVLKDYF
                     KSRAGL"
     gene            complement(4390..6627)
                     /locus_tag="VGB16_01975"
     CDS             complement(4390..6627)
                     /locus_tag="VGB16_01975"
                     /inference="COORDINATES: protein motif:HMM:NF018884.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="GAF domain-containing SpoIIE family protein
                     phosphatase"
                     /protein_id="HEX9916479.1"
                     /translation="MNFQLLVGGIYLACGIILFFLGVIILRENAQNRINRVTSLMLFF
                     AGLGPLFGALGTILKSVGYSAVLEKSLFYQNIFYFWELFFPQLLLFSLLFPEEHLFIK
                     KFSRLKYIIFIPHIFHCFLVLIFYDPSKIINFLDVEKMGSVAKIILDPISYILRSFAL
                     FLEIMYKVHVQFFSVINLLYLMLAIWFLYQGAKKVQMPHLKRQVNLILWGIRSSMGLY
                     AVAFILPVITPLRIPDALRYALTIIALVMGGGVVAWAIIRHHFLDVRLIVRQSLVYSL
                     TTLLLVGAYFLIVVEFGRWVQRVIGMQIPLLDVGYVILAIMLFQPVMGQIDDMVKRVL
                     IRDKTDYRNMMETFSREIVTLFDLENLSQKVMSTLRNEMMVEQVFLSVYDQDRKGYRL
                     FSLADQKGVIDLFSPEDVFFKILGGRNQPSLLEDLNPHKVKSVLHPNLLNLKVYMVVP
                     LKEKENLVGFIALSKKFTGFRYTYEDITLLTILANQLVIAINNTRLYAESLEKQRLEE
                     ELAVARQIQLDLLPKVFPQSSFFEISAYTSPARQVGGDYYDFLKKDDGTLAIALADAS
                     GKGIPAALLVSLVHASLRAEIKDNLPVWKTVSNINNLINSSTSAEKFVTMFYGELDPQ
                     ARRLTYCNAGHNYPILVRKGSQVQYLDVGGLILGAFAEVSYAMGDVELNNRDLIFFYS
                     DGLTDARNTQDEEFGENRLTQLLLEWSYLSTDELKEKIINEVNIFSQGTVQYDDLTFV
                     ALKVL"
     gene            complement(6887..7333)
                     /locus_tag="VGB16_01980"
     CDS             complement(6887..7333)
                     /locus_tag="VGB16_01980"
                     /EC_number="2.7.13.3"
                     /inference="COORDINATES: protein motif:HMM:NF024970.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0000155 - phosphorelay sensor kinase
                     activity [Evidence IEA];
                     GO_function: GO:0004673 - protein histidine kinase
                     activity [Evidence IEA];
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_process: GO:0007165 - signal transduction [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="ATP-binding protein"
                     /protein_id="HEX9916480.1"
                     /translation="MKEVNLRKGKIIRKGNQIIIPSSLDYLCCVDEFMEKKLKKLGVD
                     KNLLADCAISLSEVVSNAIHHGNLDNLEKKVKIELFPKPDQITFRVTDQGNGFDPKCL
                     KNPIDPECLLKEIGRGLFIVKNLMDELNFRFEPGCGTTVEITKKLK"
BASE COUNT         1987 a         1776 c         1550 g         2076 t
ORIGIN      
        1 tcgccatacc caaaatcgta tacttccact atacctggat gaactaattc cttaaggagc
       61 acaaattcgg tcttgagacc ctgttccgca gctttagagt ctgcctcagg ataagagata
      121 cgcagcgcta cttcctgttt attccagata ttataagctt tatctacctc tcccaatcta
      181 ccatggccaa gattttccaa caaacagaag cggttatcaa aaaccctttc gctcatataa
      241 cacctttctc aagacacaca attcgtggaa ttttggcaaa agacacgagg ccgtttctcg
      301 attttaggca accaacttct tatccgttat cttctgttcc tttttcaccg gaagctcgat
      361 ggtgaaggta gctcccctgc ccggaatgct gtccgtactt acgctgccac catgattctt
      421 aacgattttc tcacaagtca ccaatccaaa accatgacca tcctttttgg tggtgaaacg
      481 tggctcaaag atccggtcga tgagttcctc cggaattccc ggaccattat ctttgaccct
      541 tatctgaata actcccgatt caggacgata atgggtggaa atagtcacta tgccatcgct
      601 cttctcttgc ttgttgattg cgtctgccgc attgttaagt agattaagca gcacctgttg
      661 aatttgccca acatcgatta aaagacgggg aacctgggta tcgaagttgg tgatgaattt
      721 tatgttggag aactgctttt gaggtttaac ggagaaaaga aggtcttcga ttaagttctt
      781 aacgtcatat tccactaatt tggcttctaa cttggagaag tccataagtc catccgtgaa
      841 tctcttcatc atctctacgc tctccacgat cgatttggta ttctgatgga ctttccccag
      901 atctcctttc ttcaagttta tgggaaggag ctcggcatta ttcaatatta tggttaaata
      961 gttgttcaat tcgtggccta tggaggcagc catctccccc ttggctacca gtttctctga
     1021 aagaatcaga tatttttcta agataacttt ctcagtgata tcttcaatta cgatgatcaa
     1081 ccccatgatg cgattatcca gactgttgag cgggctgatt ttcaaagaaa gggccaactc
     1141 ctcggtctcg aatttgtgat aatagcgaga ttcctcaaaa ggttgtccag tataaagcac
     1201 ttgataacag attcttttcc agttctcctt ttccttgctg ggcaaaaggt caagtatgct
     1261 taaattggcg aagccaaact ctcccgaccc gtctttccag ccagcccttc tgaagatttc
     1321 ctgcgaggcg cgattggcaa tggttatctt cagctcatta tccactacta tgatcccgat
     1381 gggagtgcga ttcaccacgt tctcgttata tatcttcata gccaagaggc tttcatataa
     1441 cttggcattc tcgatagcga tggcggcctg gcccgcaaac aactcaaaca cgtaaaggtc
     1501 actcttcagg aagatgttag tcttggagct gttgtccaga tagagtatgc cgacaatcct
     1561 ctctttgctt ttgagtggca cgcacatgat ggatttcaaa tccaattccc tgacgctttt
     1621 ttgcctggag aatctttcgt cctgctgggc gtcactggta tagatcgatt ctccggtttt
     1681 agccacggta ttggcaatag acatacttat tctaaagtca tcactggaaa gtgattcctt
     1741 acagatatta taagcggtct tgtattgtag ctggttttcg ttatcgagga gcattaagaa
     1801 gcccctctcc gcccggagca attttatggc tctcttcatc accacctgca agatatcatc
     1861 taagaccaga gttgaattta tggctttgct gatatccaac agagcttcta aatcgtcgct
     1921 tctcaacttt ccctgttcac tgttattcga atttttcaag cgaggctggg cggtctcgac
     1981 cttgtgcagg atatcttcca tttccgtcag tatgtcgtga ggcttgtctt tcttgtcgtc
     2041 cctttttcct gcaaacttac gtttagccat ttcttctcct caaaaagaga aatacaacca
     2101 gagactaaaa ctcttttata tttatcggta gggaagttaa attatttagt gcaaattaaa
     2161 tgaattgtaa cggattaggc aaaaattgaa cagttattca tttgtcaggt gtacagggca
     2221 cctgacctgt tttttgaggt aacatcgtag gtcgggttgc gaccccgctt tttatggtga
     2281 gcgacttgtc ctcaagagaa tccgaaggaa gtcgaaccat tggcgggaaa gttacccgac
     2341 acttcactaa ccccctattg tcccccttat cagggggaca cgaatgattc ctttcctgat
     2401 tgtcaggggg atactattcg ttcctcccct gataagggga ggttaggagg ggttcaaaag
     2461 agttattggg tcgggtaaag cccccgtagg ggatctgcga ttttagcttt atattgttgg
     2521 ctcaaccttt ggtggtacac tccaaaaggg gaaacgaaaa gggtttggcc tctccctctc
     2581 taatcttttg atttgagtgg caaatccgat tcctaaacca acaaatgagt tccgcgtaag
     2641 tgtggagctt ctctccaccc gcaccccgtc gggcgaagat gaacttcgcc cctacgcgcc
     2701 agaataaacc caaaaaggta ggcgacccat atgggtcgcc tacgagaatc gggggaggat
     2761 aaattcctgc ccagaataac aaaatgagta gggacagaac actgttctgt ccctacagct
     2821 tgtcattcct gtcccgcttt aagcgagata aactccagcg gtatgaatca gaacggttca
     2881 taccagcggg aatctatatg gagtgcattg accatgtcga tgccgcatcc cgccatcggc
     2941 cgggatgcac tccagagttg caatgtcgcc tacgatggat ttaggacccg cacagggata
     3001 aattccctgt gctacaaagt agattatgct cgtaggtgtg ggaatttcct tcgaccttgc
     3061 tcaggatggt gagcgaagtc gaaccatatc ccacaccgat ttttttgcag cgaaaactcc
     3121 caccttgcct gcccggccag ggatggcaag gtgggaatac cagataagaa agacagcaga
     3181 aggcgaccca tctcggtcgc ctgcgattct atgttttcta atcaaaactg gattccgccc
     3241 taactactgg gttttcaaca tcaagtgcac aaatgacagg cgggtatttt gaaggcgaac
     3301 ccgtacgggg cgcttgcaat tttataacgt aggggcagac tttcaagtcc gcccgaaaaa
     3361 cacgggcggt tctaaagccc tgccctactt ttgaaataat ctttcaacac ctgtctatgg
     3421 gcggcaaaag cgatattagt gggaatttcg tcttgactga agaaggatat ttcctgggcg
     3481 tcatctcccg gctttggctc gccttcgatt atgtctgcta aataaactac caaaatcgca
     3541 tgggtgcgtg gatcgtcatg gccccagtaa actccaaaga gaccggagag tctgatttta
     3601 agattggtct cctcgtaaat ttctcttatg gcacaagctt ccggtgattc cccatattcc
     3661 ataaatccag ccggaaaact ccaatccccc ttaaatggag gaaattttct cttaaccaaa
     3721 agcaccttgc cgtccttttc tattatcact ccgctagccg gagcgggatt tctgtaaagg
     3781 acgaacccgc ataccggaca gcattttcta atttttccat ctgctttctt ttccaccagt
     3841 tcactggtgc aagccggaca atatctataa attggacgga gatttctccc tgtccggaga
     3901 caaggtctct gatactcatt ttcctctgtg tttcgagttt tctttagttt acctctctga
     3961 taaatcttac cgctgggttt aatgcgggta accgggttaa tactccaggt tcttcttctg
     4021 cttttgtgtt ttgcagtctt tttcattttt acccgcctct gcagaaagcc acgcctgtca
     4081 agacgtagac gccgtcggtc aatagtctat ggcgtcggac gatgaatgcg gaaagggtat
     4141 ccgctgaagt ccgtctttgc tcaattgctt tcgcattgag cctcaccgag atcccgccga
     4201 agcttgaaaa gcgaaggcgg actaaaggcg aaaccgcttt agcgtaggcg gatatgcttc
     4261 ggcgggtttc gcttaaagca agtgaagtcg ccgacgctct aaagctatgg cgacaaagcg
     4321 atgccacgac tgtccaacct tcgcagccga agcggatgct tcggcggagc aggctaaaac
     4381 cgtggagctt cacaaaacct ttaaggcgac gaaggtcaaa tcatcatatt gaactgttcc
     4441 ctgactgaaa atatttacct catttataat cttttcttta agttcatctg tagaaagata
     4501 agaccactcg aggagaagct gagttaatct gttttccccg aattcctcat cttgcgtgtt
     4561 tctggcatct gtcaaaccat cactgtaaaa gaaaatcaaa tctcgattat taagctccac
     4621 atctcccatc gcgtaggaga cttctgcaaa agcgcccaag atcaaaccgc ccacatccaa
     4681 atactgaact tgacttcctt ttctgactaa aatcgggtag ttatgaccag cgttacagta
     4741 agtcaatctt cgggcctggg ggtctagctc tccgtaaaac atggtaacga atttctctgc
     4801 tgaggtgctg gaatttatca gattatttat gttggacacg gtcttccaga caggaaggtt
     4861 atcttttatc tccgccctca gggaagcatg caccagagaa acgagcaaag ctgcggggat
     4921 tcccttgcct gaagcatccg ctaaggcaat agccagagtg ccatcatctt ttttcaggaa
     4981 atcgtaataa tcaccaccca cctgacgagc agggctggta taagctgata tctcaaaaaa
     5041 gctactctgc ggaaaaacct tgggcaaaag atctaactga atctgacgag ctacagccag
     5101 ctcctcttcc agtctctgtt tctccaggga ttcggcataa agcctggtat tgttgatagc
     5161 tataactaac tgattagcca gtatggtgag caaagttata tcctcataag tatatctgaa
     5221 tccggtgaat ttcttgctta atgctatgaa accaacaaga ttttcttttt ccttcaaagg
     5281 caccaccata tagactttca aattcaagag attcggatga agtacgctct tcaccttgtg
     5341 gggatttaaa tcctccaaaa gagagggttg gtttctgcca cccaggattt tgaagaaaac
     5401 atcctccgga ctaaaaaggt caattactcc tttttggtca gccagagaaa aaagacgata
     5461 ccccttccta tcttggtcat agactgacaa gaacacctgt tccaccatca tctcgtttct
     5521 taaagtgctc atcaccttct ggctcaagtt ttcgagatca aatagggtta ctatttcccg
     5581 actgaaggtt tccatcatat tacgataatc cgttttgtcg cgaatcaaga cccttttgac
     5641 catgtcatct atttgcccca tcaccggctg gaatagcatt atagccaaga taacataacc
     5701 tacatccaga agcggaatct gcatgccgat tactctttgg acccatctgc caaactccac
     5761 cactattaaa aagtaagctc ctaccaagag caaagtagtt agggaataaa ccaaactttg
     5821 acgcacgatt aggcgcacat ccaaaaaatg atgcctgatt atagcccagg ctacaacccc
     5881 acctcccatc accagagcga ttatggtcaa agcgtaacgt aaggcatcgg gaatccgtaa
     5941 gggggttata actggaagga tgaaagccac ggcgtaaaga cccatagatg atcttattcc
     6001 ccacagaatt agattaacct gtcttttcaa atgaggcatt tgaacttttt ttgcaccttg
     6061 atacaagaac catatagcca acatcaaata gagcaggttt attactgaaa agaactgaac
     6121 atgcacttta tacataattt ctaaaaacag ggcgaacgat ctcaaaatat aggaaatggg
     6181 gtccaggatt atcttagcta ccgagcccat cttctccacg tccaggaagt ttatgatctt
     6241 actggggtca taaaatatca aaaccagaaa gcaatgaaag atatggggga tgaaaatgat
     6301 atatttcagc cgagaaaact tttttatgaa gaggtgttcc tcgggaaaaa gtaacgaaaa
     6361 gagcaaaagc tgggggaaga aaagctccca gaaataaaat atgttttgat aaaaaagtga
     6421 tttctctaaa acagcggagt aacccactga tttgaggatt gtgcccaaag ccccgaagag
     6481 tggacccaag ccggcaaaaa acagcatcaa gctggttacc cgattgattc tgttctgggc
     6541 attctcgcgc aggattatca ctcccagaaa aaagaggatg atcccgcaag ccagatatat
     6601 gcccccaacc aataattgaa aattcatctc ttcgctcttc ctaagtcccc ccgtaggggg
     6661 atgaattatg tccttttagt tccctctccc caatggggag agggatagag cctgccctga
     6721 gcgaagcgaa ggggtgaggg ggagaaaaga aagaaccttc actctcactc cgccttggcg
     6781 ggattttcaa ctttatcctc tccctcaagg gagaggagat tgtttttcaa agaggtccta
     6841 tggatccaga ttcggacaag atgcctcgcc ctttggccgg ggagtttcac ttgagctttt
     6901 ttgttatctc cacggtagtg ccacaacctg gctcaaacct gaagtttaac tcatccatca
     6961 gattcttgac gatgaaaagt cctctcccta tctccttaag taaacactcc gggtcaatcg
     7021 ggtttttcag gcattttggg tcaaatccat ttccctggtc tgtgacccga aaggttatct
     7081 ggtccggctt tggaaataac tctatcttca ctttcttttc caggttatcc aaattaccat
     7141 gatggatggc attgcttaca acttcactca aggagatagc gcaatcagcc aaaagatttt
     7201 tatccacacc caatttcttc agcttctttt ccataaattc atccacacaa cacagataat
     7261 ccaaggagct tggaattatg atttgatttc cttttcttat gattttgcct ttgcgcaagt
     7321 tgacctcttt catagattct cacaccttta tggttcgacc ttgctcacca tcctgagcat
     7381 gtcgaagga
//
LOCUS       DASWLN010000053         1991 bp    DNA     linear   ENV 20-FEB-2024
DEFINITION  MAG TPA_asm: Candidatus Zixiibacteriota bacterium isolate
            SMAG_U2949 k141_52871, whole genome shotgun sequence.
ACCESSION   DASWLN010000053 DASWLN010000000
VERSION     DASWLN010000053.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37960594
            Sequence Read Archive: SRR10376091
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Candidatus Zixiibacteriota bacterium (soil metagenome)
  ORGANISM  Candidatus Zixiibacteriota bacterium
            Bacteria.
REFERENCE   1  (bases 1 to 1991)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 1991)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (26-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/12/2024 08:28:26
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 1,440
            CDSs (total)                      :: 1,416
            Genes (coding)                    :: 1,412
            CDSs (with protein)               :: 1,412
            Genes (RNA)                       :: 24
            rRNAs                             :: 1 (16S)
            partial rRNAs                     :: 1 (16S)
            tRNAs                             :: 21
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 4
            CDSs (without protein)            :: 4
            Pseudo Genes (ambiguous residues) :: 0 of 4
            Pseudo Genes (frameshifted)       :: 0 of 4
            Pseudo Genes (incomplete)         :: 3 of 4
            Pseudo Genes (internal stop)      :: 1 of 4
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..1991
                     /organism="Candidatus Zixiibacteriota bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_52871"
                     /isolate="SMAG_U2949"
                     /isolation_source="soil"
                     /db_xref="taxon:2053527"
                     /environmental_sample
                     /country="USA: Oregon"
                     /lat_lon="42.99 N 123.63 W"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            <1..639
                     /locus_tag="VGB16_01985"
     CDS             <1..639
                     /locus_tag="VGB16_01985"
                     /EC_number="5.2.1.8"
                     /inference="COORDINATES: protein motif:HMM:NF024545.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003755 - peptidyl-prolyl cis-trans
                     isomerase activity [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="peptidylprolyl isomerase"
                     /protein_id="HEX9916481.1"
                     /translation="SEILYEMAIKQKVDKKLEFLIEQSKKDVVINKFLEENLKDIGIT
                     PEEISTFYNSNKDNFKRSWDEVRASHILLLKKEDAQRALKRIESGEDFAKVAKDMTED
                     QNTRMQGGDLGYFGALEVSPQIAQMAFTLPLGKVSPIIQTEQGFDIIKLFDKKPKGSL
                     RGLEEVKSQIGLILLNQKRTERIEKIAEEGKSNLKIERFNWAIDSTTLTTKE"
     gene            647..1936
                     /locus_tag="VGB16_01990"
     CDS             647..1936
                     /locus_tag="VGB16_01990"
                     /EC_number="5.2.1.8"
                     /inference="COORDINATES: protein motif:HMM:NF025010.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="peptidylprolyl isomerase"
                     /protein_id="HEX9916482.1"
                     /translation="MKRIALVVMAILCLVWFSLSLAQEVLDRIVAVVGNQIILESELK
                     TQIQLYGDQFLQSGISQDELESTILGQMIKDKLILIQAEKDTSIKITSEQVEEALNEQ
                     MKRLKERFASEEEFQAQLHKEGLTEKELRKRYKKAINDQLLRDKLLSSKVSKISVSNK
                     EVRDFYYNYQDSIPEQPEGVRLGHILIPIKTGPEIIDQLKKKAQSILEMAKKGEDFSE
                     LAKKYSDDPSSAQGGELGYFKRGDMVPSFERAAFALKPGEMSDVVETQFGFHIIKLED
                     KKDNMVKAQHILIMLRPSTEDTATAIQLAQEIYSKIEARADFAELAKAYSGDEESKKM
                     GGDLGWYAVSSLSPEFQKAIEGLEIGQTSQPLVTQFGVHLLKVSDKKEGKRLTLEEDW
                     DTIKDMVKRQKADKVIGEWVDGLMQKTYVEIKLTENK"
BASE COUNT          676 a          345 c          500 g          470 t
ORIGIN      
        1 tcggagatac tttatgaaat ggcaataaag caaaaagtgg acaagaagct cgaatttcta
       61 attgaacaat ccaaaaagga tgtggtgatt aataaattcc tggaggaaaa tctcaaggat
      121 atcggaataa ctccggaaga gatcagcacc ttctacaact ccaataaaga taactttaag
      181 aggtcttggg atgaggttcg ggctagccat attcttctct taaaaaaaga agatgcccaa
      241 agagctttga aaaggattga aagtggggag gattttgcca aggtggcaaa ggatatgact
      301 gaagaccaaa acacccgcat gcaaggcggg gatctgggat atttcggcgc tcttgaggta
      361 tcccctcaaa tagcacagat ggctttcact cttccgcttg gcaaggtctc acccataatc
      421 cagacggagc aaggttttga cataataaaa ctgtttgaca agaagcctaa ggggagctta
      481 agaggtttgg aagaagtcaa aagccagata ggacttattc ttctcaatca gaagagaacg
      541 gagagaatag agaaaatagc ggaggaaggc aaatccaatt tgaaaatcga gagattcaat
      601 tgggctatag attcgactac gctcactaca aaggagtgac taaaagatga aaagaatcgc
      661 tttggtagtt atggccattt tatgtttggt ttggtttagt ctctcattag cacaggaggt
      721 gctggaccgc atcgtagcgg tggtggggaa ccaaatcatc ctcgaatctg agcttaagac
      781 ccaaattcag ctttatggcg accaattctt acagagtggg attagtcagg atgagctgga
      841 aagtaccatc ctgggtcaga tgattaaaga taaactgatt ctcattcagg cggaaaagga
      901 caccagtata aaaatcacct ctgagcaggt ggaagaggct ttgaatgagc agatgaaaag
      961 acttaaggag cgttttgcca gtgaagaaga gtttcaagca caactgcata aagaagggtt
     1021 aactgaaaag gagctaagaa agagatacaa gaaggcgatc aacgatcaat tattaagaga
     1081 caagctcctc tcttccaaag tatccaagat cagcgtttcc aataaagagg taagggattt
     1141 ctattataat tatcaggata gcattccgga gcaacccgag ggggtaaggt taggtcatat
     1201 actcatcccc attaaaaccg ggcccgagat aattgaccag cttaagaaaa aagcacagtc
     1261 catactggaa atggcaaaaa aaggtgaaga cttttcagaa ttggcaaaaa agtattcgga
     1321 tgacccttca tctgcccagg gtggggaatt gggatatttc aaaagagggg atatggtgcc
     1381 ttcttttgaa agagcagctt ttgcgttgaa accgggagag atgagcgatg tggtggaaac
     1441 tcaattcgga tttcatatca taaagctcga agacaagaaa gacaatatgg tcaaagccca
     1501 gcacatcttg attatgttga gaccctcaac agaagatact gccacggcca tccagttagc
     1561 gcaagagatt tattccaaga tagaagcaag ggctgatttc gctgaattgg ccaaggccta
     1621 ttctggtgac gaggagtcca agaagatggg aggagattta ggttggtatg cagtttcgtc
     1681 cctttccccg gaatttcaaa aagcgataga gggtttagaa attggtcaga ccagccaacc
     1741 tttagtaact caatttggcg tgcatctctt aaaagtatcg gacaaaaaag aagggaaaag
     1801 attaactttg gaagaggatt gggacacaat caaggatatg gtcaaaaggc agaaagcgga
     1861 caaggtgata ggggaatggg tggatggttt aatgcagaaa acctatgtgg agataaagct
     1921 gactgaaaat aaataggggg caatcttttg cgtttagata cttttttgtc aatctcccgc
     1981 ctgataaaaa g
//
LOCUS       DASWLN010000054         3458 bp    DNA     linear   ENV 20-FEB-2024
DEFINITION  MAG TPA_asm: Candidatus Zixiibacteriota bacterium isolate
            SMAG_U2949 k141_151217, whole genome shotgun sequence.
ACCESSION   DASWLN010000054 DASWLN010000000
VERSION     DASWLN010000054.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37960594
            Sequence Read Archive: SRR10376091
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Candidatus Zixiibacteriota bacterium (soil metagenome)
  ORGANISM  Candidatus Zixiibacteriota bacterium
            Bacteria.
REFERENCE   1  (bases 1 to 3458)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 3458)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (26-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/12/2024 08:28:26
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 1,440
            CDSs (total)                      :: 1,416
            Genes (coding)                    :: 1,412
            CDSs (with protein)               :: 1,412
            Genes (RNA)                       :: 24
            rRNAs                             :: 1 (16S)
            partial rRNAs                     :: 1 (16S)
            tRNAs                             :: 21
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 4
            CDSs (without protein)            :: 4
            Pseudo Genes (ambiguous residues) :: 0 of 4
            Pseudo Genes (frameshifted)       :: 0 of 4
            Pseudo Genes (incomplete)         :: 3 of 4
            Pseudo Genes (internal stop)      :: 1 of 4
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..3458
                     /organism="Candidatus Zixiibacteriota bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_151217"
                     /isolate="SMAG_U2949"
                     /isolation_source="soil"
                     /db_xref="taxon:2053527"
                     /environmental_sample
                     /country="USA: Oregon"
                     /lat_lon="42.99 N 123.63 W"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            complement(<1..1976)
                     /locus_tag="VGB16_01995"
     CDS             complement(<1..1976)
                     /locus_tag="VGB16_01995"
                     /inference="COORDINATES: protein motif:HMM:NF012298.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004672 - protein kinase activity
                     [Evidence IEA];
                     GO_process: GO:0006468 - protein phosphorylation [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="protein kinase"
                     /protein_id="HEX9916483.1"
                     /translation="MIGKIISHYRILEKLGEGGMGVVYKARDIKLDRIVALKFLPRHL
                     LCDSEAKTRFEHEAKSASALNHTNITTIYEIDEIEGECFIAMEYIEGKSLKKLIEEKT
                     FTLKEVLDVAIQVSEGLAIAHEKGIVHRDIKSDNIMLTPRGRVKIMDFGLAKLKGITD
                     LTKTGTTMGTVAYMSPEQARGEAIDHRTDLWSLGVVIYEMITKQLPFKSEYEQAVIYA
                     ILNEEPKPMTETRPEIPSELEQIVRKALAQNPDSRYQNAGDVLVDLEKLKPSILKREF
                     SSAKPQPSIAVLPFTNLSADKEQEYFCDGMAEEIINALTQVEGLHVVARTSAFFFRGK
                     EIDIREIGRKLNVGAVLEGSVRKAGNRLRITAQLVNVADGYHLWSERYDREMEDVFAI
                     QEEISLSIVDKLKGKLLKEEKVKLVKRYTDNPEAYNLYLKGRYFWNRRYEGGLQKAVE
                     CLQEAIDKDPLYALAYAGIADCYNQFGLWSFLHSKEAYPRAKVACAKALEIDDTLAEA
                     YASLGWIKTFYDWDWAEAEKAFKRALELNPNYAAAHYFYGLYLGIMGNGVEAIAEAKK
                     SVELDPLCLILNAVLGLSLYWWRQYDEAIEQLHKTLEMDPNFAIAHFYLGLSYAGKQR
                     WKESIISFQEFVNLWPGSPIPVGYLGFAYGMSGQ"
     gene            complement(2278..>3458)
                     /locus_tag="VGB16_02000"
     CDS             complement(2278..>3458)
                     /locus_tag="VGB16_02000"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_014063274.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=3
                     /transl_table=11
                     /product="excinuclease ABC subunit UvrA"
                     /protein_id="HEX9916484.1"
                     /translation="ETADYVIDLGPGAGKQGGEMVACGSPLSIKRNNKSLTGMYLSGK
                     LSIPVPEKRRVGYGDNLSIMGASGNNLKKIKVDIPLGIFTCITGVSGSGKSTLINETL
                     YRILAHYFYGSNILPLKYEKITGLDKIDKVINIDQSPIGRTPRSNPATYTGVFTFIRD
                     LFSQIPEAKVRGYKAGRFSFNVKGGRCEACEGDGIIKIEMHFLPDVYVPCEVCKGKRY
                     NRETLEIKYKGKNIAEVLDMTVDEAFYFFENIPNIRRKLVTLKEVGLGYIHLGQSATT
                     LSGGEAQRVKLSTELSKVSTGRTLYILDEPTTGLHFEDIKMLLRVLDRLVEMGNTVVV
                     IEHNLDVIKAADYIIDLGPEGGDEGGEVIAFGTPEKVAQNGNSYTGKFLKKILNLSEK
                     "
BASE COUNT          960 a          864 c          604 g         1030 t
ORIGIN      
        1 tcttgcccgg acataccata agcaaatcca agatatccaa ctggaattgg gctgcctggc
       61 cagagattca cgaactcttg gaaagaaata atagattctt tccacctctg tttccccgca
      121 taggataatc caagataaaa atgggcaata gcaaagtttg gatccatttc gagagtcttg
      181 tggagctgtt ctatggcttc atcgtactga cgccaccagt aaagggaaag tcccaaaacc
      241 gcattcaaga taagacaaag cgggtctaac tccacacttt ttttcgcctc agcgattgct
      301 tcaacaccat tccccattat ccccagatac aaaccataaa aatagtgggc tgcagcataa
      361 ttagggttaa gctctaaggc tcgtttgaat gctttttcag cctctgccca gtcccaatcg
      421 tagaacgttt taatccagcc cagcgaggca taagcttcgg caagtgtgtc atcaatttcc
      481 aatgccttag cgcaagcaac ctttgctctg ggataagcct cttttgaatg aaggaagctc
      541 caaagtccaa actgattata acagtcagct atccccgcat aggccagtgc atagagtggg
      601 tctttgtcaa tggcttcctg taaacactca actgcttttt gcagtccacc ttcataccgc
      661 ctgttccaga aatagcgacc tttcaagtag aggttataag cttccggatt atcggtgtaa
      721 cgctttacca gttttacttt ctcctctttc aaaagcttcc cttttaactt atccacaatt
      781 gacaaagaga tctcttcctg aatagcaaat acatcctcca tctctcgatc atatcgctct
      841 gaccaaaggt ggtaaccatc agccacatta accagttggg cagtaatacg gagcctgttc
      901 cctgctttac gcacactccc ttcaagtact gctcctacgt tgagctttct gccgatctct
      961 cgaatatcta tttcttttcc cctaaagaag aaagcagagg ttcgagcaac cacatgcaaa
     1021 ccctccacct gtgtcagagc attgataatc tcctcagcca taccgtcaca gaaatattcc
     1081 tgctctttat ccgcactcag attggtaaat ggcaacaccg caatggacgg ctgaggcttg
     1141 gctgaagaaa actctctttt caaaatgctg ggtttgagct tctctaaatc aaccagaaca
     1201 tctcctgcgt tctggtagcg agaatcagga ttttgggcga gcgcttttct tacaatctgc
     1261 tccagttccg atggaatctc aggtcgtgtt tccgtcatcg gttttggctc ttcattaagg
     1321 atagcataaa tcactgcctg ttcgtattca ctcttaaagg gcaattgctt agttatcatt
     1381 tcgtatatca ccactcccaa agaccagaga tctgtgcgat gatctattgc ttctccacgt
     1441 gcttgctctg gtgacatata agctacagtt cccatggtag tccctgtttt agttaaatca
     1501 gttattccct taagctttgc cagaccaaaa tccattatct taaccctccc tcgaggagta
     1561 agcattatgt tatcagattt gatatcccga tgtacaatcc ccttctcatg ggcaattgct
     1621 aaaccttcac ttacctgaat agcaacatcc aggacttcct tcagagtaaa ggttttttct
     1681 tcaatcaatt tctttagaga tttcccttcg atatattcca ttgcaataaa acattcaccc
     1741 tctatctcat ctatctcata aattgtggtg atattggtgt gattcaaagc agaggcggat
     1801 ttagcctcat gctcaaatct ggtcttcgct tcgctgtcac acagaagatg tcttggcaag
     1861 aacttcaaag ccacaatcct atccagctta atgtcccggg ctttatagac cacgcccatc
     1921 cccccctcac ccaatttctc taaaatcctg taatgtgaaa tgattttacc tatcataaca
     1981 gaactttggt atctgctttg gtagtcgcag actttagtct gcgttttttt atttcgcaag
     2041 ttaaagcttt cagccacctg cctggtaata caagaaacat cccaccaata acatcctgcc
     2101 tctgataaga ctcgactatg cccttaataa attgagaaat ttttaattgt caaccaagaa
     2161 atatgaggta aaattaaata tatgtgtgtg aatgtttgtc aaaaagaagg attacgaaaa
     2221 aaattggtaa aaccgccgtg ccgtccctac aatctatccg tcatctgata ttagttatta
     2281 tttttcgctc agatttaaaa tcttctttaa gaatttacca gtataggaat tcccattttg
     2341 tgctactttt tcaggtgtgc caaaggcaat aacctctcct ccctcatctc caccttcagg
     2401 acccaaatct atgatataat ctgcagcttt aatcacgtcc aagttatgct cgataactac
     2461 cacagtattt cccatttcca ccagccggtc taaaacccgc aataacatct ttatatcctc
     2521 gaaatgtaaa ccggtagtag gctcatctaa aatatataag gtcctgccag tgctaacttt
     2581 ggaaagctca gtggagagtt taaccctctg ggcttcccct ccggataagg tcgtagctga
     2641 ttggcctaaa tggatatatc ccagaccgac ttctttcaaa gtaacaagct ttctacggat
     2701 attaggtata ttctcaaaaa aatagaatgc ctcgtccaca gtcatatcca ggacctcagc
     2761 tatattcttc cccttatatt ttatctccag cgtttcccga ttatatcttt tccctttgca
     2821 gacctcacag gggacataaa catccggtag aaaatgcatc tcgatcttaa ttatcccatc
     2881 cccttcacaa gcttcacatc tccccccttt gacgttgaag ctgaacctcc cagccttata
     2941 ccctctgacc ttagcctcgg gaatttgaga gaaaagatcg cgaataaaag tgaaaacccc
     3001 ggtataagtt gcaggattag accgaggggt tcttccgatt ggagactggt cgatattaat
     3061 aaccttgtct attttatcca gtccggttat cttctcatat tttaggggga gtatattaga
     3121 accataaaaa tagtgtgcca gaatgcgata taaggtctca ttaatcaggg tcgattttcc
     3181 agaaccggat acaccggtta tacaggtaaa aattcccagg gggatatcca ctttaatctt
     3241 tttaagattg tttcccgaag cacccataat ggatagatta tctccatacc ctacccttct
     3301 tttttctgga accgggatag agagctttcc tgaaagatac atcccggtca aagatttatt
     3361 atttcttttg atactcaaag gagatccgca ggcaaccatt tcccctcctt gtttaccagc
     3421 acctggaccc agatcgatca cataatcagc agtttcaa
//
LOCUS       DASWLN010000055         1897 bp    DNA     linear   ENV 20-FEB-2024
DEFINITION  MAG TPA_asm: Candidatus Zixiibacteriota bacterium isolate
            SMAG_U2949 k141_943778, whole genome shotgun sequence.
ACCESSION   DASWLN010000055 DASWLN010000000
VERSION     DASWLN010000055.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37960594
            Sequence Read Archive: SRR10376091
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Candidatus Zixiibacteriota bacterium (soil metagenome)
  ORGANISM  Candidatus Zixiibacteriota bacterium
            Bacteria.
REFERENCE   1  (bases 1 to 1897)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 1897)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (26-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/12/2024 08:28:26
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 1,440
            CDSs (total)                      :: 1,416
            Genes (coding)                    :: 1,412
            CDSs (with protein)               :: 1,412
            Genes (RNA)                       :: 24
            rRNAs                             :: 1 (16S)
            partial rRNAs                     :: 1 (16S)
            tRNAs                             :: 21
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 4
            CDSs (without protein)            :: 4
            Pseudo Genes (ambiguous residues) :: 0 of 4
            Pseudo Genes (frameshifted)       :: 0 of 4
            Pseudo Genes (incomplete)         :: 3 of 4
            Pseudo Genes (internal stop)      :: 1 of 4
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..1897
                     /organism="Candidatus Zixiibacteriota bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_943778"
                     /isolate="SMAG_U2949"
                     /isolation_source="soil"
                     /db_xref="taxon:2053527"
                     /environmental_sample
                     /country="USA: Oregon"
                     /lat_lon="42.99 N 123.63 W"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            complement(<1..906)
                     /locus_tag="VGB16_02005"
     CDS             complement(<1..906)
                     /locus_tag="VGB16_02005"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+.
                     GO_function: GO:0005515 - protein binding [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="fibronectin type III domain-containing protein"
                     /protein_id="HEX9916485.1"
                     /translation="MHKKIKTTIILALLISMCLLGCSRKIKNPVSDYGEMEAPPTPQS
                     VIVLVGDSKVELNWEVSDSSKVERFKIYRSDTSSITPFLIDSCFGFSYVSRGLKNGKE
                     YFYQVSAVDSNGFEGYRSETVSATPNLFSVVINNGQKVTSSRDVTLSLAAPSGTSYMM
                     VSDDSLFSGASWERYSLSKSWKLEPGDGEKWVYVKFKDADGNVCIDFFQSSIILDTQA
                     HIISVSENTGEQIKTTGQTIHFRLNSGEANGQARVDLGSISNIILYNNGQFGDTVAND
                     SIYELDYVIPPGVEMENATVTGHFTD"
     gene            complement(926..1669)
                     /locus_tag="VGB16_02010"
     CDS             complement(926..1669)
                     /locus_tag="VGB16_02010"
                     /inference="COORDINATES: protein motif:HMM:NF040144.3"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF5683 domain-containing protein"
                     /protein_id="HEX9916486.1"
                     /translation="MSTKRISTMLSLGLVIWMNSSVLAQQSGTKDSYLDIVSNPSGAT
                     VRLKGDYDLIVTAPCGINQELSGEYNIKAFKRGYESWSSRVWFNQGNPHQLTIDLKSK
                     NRLKAGLRSALIPGWGQFYCEEKGKSFIMGISTLGGVVAFVLADNDYSNKYDDYVWAR
                     NRFDSNKEAEKWEEYKQLLDEKQREAYDAENLRRATLGIAIGIWAYNVLDAVLFFPYN
                     EEETLATKESKVALELNQGTPSLVFTKKF"
     gene            complement(1708..>1897)
                     /locus_tag="VGB16_02015"
     CDS             complement(1708..>1897)
                     /locus_tag="VGB16_02015"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=2
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEX9916487.1"
                     /translation="DSHPLSAFGRDFQLSPPPQGGGDFLDSHLLGGIVSLTEVFRRPR
                     FFVKNTLIYETVTNIFFG"
BASE COUNT          510 a          477 c          353 g          557 t
ORIGIN      
        1 atccgtgaag tgtcctgtta ctgtagcatt ctccatctcc acgccggggg gaattacata
       61 gtctaactcg tagatgctgt catttgctac tgtatctccg aattgtccat tattgtagag
      121 aattatattg cttatacttc ctaaatcgac tctggcttga ccatttgctt ctccactgtt
      181 tagtctgaaa tgaattgtct gacctgtagt tttaatttgt tcgccggtat tctctgatac
      241 gctaataata tgtgcctggg tatccagtat aatcgaactt tggaagaaat ctatgcaaac
      301 atttccgtct gcgtccttga atttaacata aacccatttc tcgccatctc caggttccaa
      361 tttccagctt ttggacaaac tgtatctctc ccaggaggcg ccggaaaaga gcgaatcatc
      421 gctgaccatc atataggaag tcccgctggg cgccgctaag ctcaaagtca catcccggct
      481 gcttgtcact ttttgaccgt tatttatgac cacggaaaaa agattcggcg tagctgaaac
      541 tgtctcggat ctgtatccct caaagccatt tgaatccact gcggagacct gatagaagta
      601 ttcctttcca tttttaagtc cacgactgac ataactgaaa ccaaaacaag agtcaataag
      661 aaagggcgta atagagctcg tatctgagcg gtaaatcttg aatctttcga ctttggatga
      721 atcgctaacc tcccaattca gttccacctt actgtctccc accagaacga tgacactctg
      781 gggagtaggt ggagcttcca tttctccgta gtcgctaacc ggatttttaa ttttcctgct
      841 acaacccagg aggcacatcg aaatgagtaa agcaagaatt atcgttgtct taattttttt
      901 gtgcattatc tcctccggaa atcaactaaa atttcttggt gaaaaccaaa cttggcgtgc
      961 cttggttcag ttctaaagct accttgctct ctttagtagc taaggtttct tcttcgttat
     1021 agggaaagaa taagactgca tccagaacgt tgtaagccca gattccgatt gctattccta
     1081 aggtagctct cctcagattc tcagcatcgt acgcttccct ttgcttctca tccagcagtt
     1141 gcttatattc ttcccacttc tcggcttctt tattactatc aaaccgattt ctagcccaaa
     1201 cataatcgtc atacttgttg gaataatcgt tgtccgcaag cacaaaagcc accactccac
     1261 ccagagtcga tatccccata atgaaacttt tccctttctc ctcgcaatag aactgtcccc
     1321 acccgggaat cagcgctgag cgcaaacctg ctttgagcct gttcttagac ttaaggtcaa
     1381 tagtaagctg atgtggattg ccctggttga accaaactct tgaactccaa ctctcatatc
     1441 ctcttttgaa agccttgatg ttatattccc cgcttagctc ctggtttatc ccacagggtg
     1501 ctgtcacaat caggtcatag tctcccttta aacgtaccgt agcgccgctc ggattggaaa
     1561 cgatatccag ataagaatcc ttcgtgccag actgttgagc caaaacagaa gaattcatcc
     1621 agatgactaa tcctaaactc agcatagttg aaatcctctt tgtgctcata ttccttctcc
     1681 ctctactgaa cagtttaccg attcacttca tccaaaaaag atattggtaa ctgtttcata
     1741 aatcaaggta tttttcacaa aaaaccgagg tcttcgaaaa acctcggtca agctaacaat
     1801 cccgcccaaa aggtgggaat ccaaaaaatc tcctccccct tgagggggag gactaagttg
     1861 aaaatcccgc ccaaaggcgg agagagggtg agaatca
//
LOCUS       DASWLN010000056         1549 bp    DNA     linear   ENV 20-FEB-2024
DEFINITION  MAG TPA_asm: Candidatus Zixiibacteriota bacterium isolate
            SMAG_U2949 k141_437538, whole genome shotgun sequence.
ACCESSION   DASWLN010000056 DASWLN010000000
VERSION     DASWLN010000056.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37960594
            Sequence Read Archive: SRR10376091
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Candidatus Zixiibacteriota bacterium (soil metagenome)
  ORGANISM  Candidatus Zixiibacteriota bacterium
            Bacteria.
REFERENCE   1  (bases 1 to 1549)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 1549)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (26-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/12/2024 08:28:26
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 1,440
            CDSs (total)                      :: 1,416
            Genes (coding)                    :: 1,412
            CDSs (with protein)               :: 1,412
            Genes (RNA)                       :: 24
            rRNAs                             :: 1 (16S)
            partial rRNAs                     :: 1 (16S)
            tRNAs                             :: 21
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 4
            CDSs (without protein)            :: 4
            Pseudo Genes (ambiguous residues) :: 0 of 4
            Pseudo Genes (frameshifted)       :: 0 of 4
            Pseudo Genes (incomplete)         :: 3 of 4
            Pseudo Genes (internal stop)      :: 1 of 4
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..1549
                     /organism="Candidatus Zixiibacteriota bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_437538"
                     /isolate="SMAG_U2949"
                     /isolation_source="soil"
                     /db_xref="taxon:2053527"
                     /environmental_sample
                     /country="USA: Oregon"
                     /lat_lon="42.99 N 123.63 W"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            294..1280
                     /locus_tag="VGB16_02020"
     CDS             294..1280
                     /locus_tag="VGB16_02020"
                     /inference="COORDINATES: protein motif:HMM:NF013752.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003677 - DNA binding [Evidence IEA];
                     GO_function: GO:0004803 - transposase activity [Evidence
                     IEA];
                     GO_process: GO:0006313 - DNA transposition [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="transposase"
                     /protein_id="HEX9916488.1"
                     /translation="MRKQSGGVWHIAEIIADDLEGRETGLSKPQRIGLADIAASVLSC
                     RSVNTSELANILPRSVKSSEESYRYISRWLSNPKIKPIEVMKGFVPEILYALTSNNQT
                     AILMLDQSKIGNGFECLMVSLRLGERAIPVAWRVKKTEGEMGYDEQEPLLKAVYDIIP
                     KGIEVMLAADRFYGTAALISLCQRLGWKYRIRLKGNLNLFHQEGWVMNPHEAKKLDLK
                     GLENVRLGEKGPLINVGILHESGHPEAWYIAMDDKPSEARTLDYGLRWGIEALFSDLK
                     TRGFSVTKTQLKQVDRIERLLLVLTIALYWAVSTGMMPREAHVNSKKKRKDL"
     gene            1438..>1549
                     /locus_tag="VGB16_02025"
     CDS             1438..>1549
                     /locus_tag="VGB16_02025"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003506036.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="acyl-CoA thioesterase"
                     /protein_id="HEX9916489.1"
                     /translation="MTETPPPIEQLTLRMIAMPRDTNPAGDIFGGWLVSIM"
BASE COUNT          459 a          297 c          341 g          452 t
ORIGIN      
        1 tttgtgtttc caataattcc aaagggtata accagcggtt cctaaaacaa tagccaccgc
       61 cagcgcaatg acatagtttc catatttctg ccataaaaca aacatccgtt cttcacggat
      121 gtcttcttca agctctctaa aaaaatctga catattcagg ctccattttc tgtccttacc
      181 ataatttaag tatttccatt ggtaaagggc cattaaggaa tttgcttaac acctgacata
      241 tttttataaa gctcaaacta tgataggaat tctcccaaaa agaggaggat tccatgagga
      301 agcaaagtgg tggtgtatgg catatagcgg aaattatagc tgatgatctc gagggccgtg
      361 agactggcct aagcaagcca caaaggattg gcttagcaga tatagccgca agtgttttaa
      421 gttgtcggtc agtcaataca tcagagttag cgaatatcct tccgcgttca gtcaaaagtt
      481 cagaagaaag ttatcgttat atcagccgct ggctttcaaa cccaaagatc aagcctatag
      541 aggttatgaa ggggtttgtg cctgagattt tatatgcact tacctcgaat aaccaaacag
      601 ctattttgat gctggatcaa agcaaaattg gcaatggttt tgagtgtctc atggtttctt
      661 taagacttgg ggaacgagca attcctgttg catggagagt taagaaaaca gagggagaga
      721 tgggctatga tgaacaagag cctttattaa aagcggtcta cgatataatc cccaaaggta
      781 ttgaagttat gttggctgca gaccgttttt atggcacagc ggcccttata tctttatgcc
      841 aacgcttagg gtggaagtat cgcataaggt tgaagggcaa tctcaatctc tttcatcaag
      901 aaggatgggt tatgaaccct catgaagcaa aaaagctaga cttaaagggg cttgagaatg
      961 ttcgattagg cgaaaagggt cctttaatta atgttggcat tctgcacgag tcaggacatc
     1021 cagaagcctg gtatattgct atggatgata aaccatctga ggcacgcacc ttggattatg
     1081 ggttgcggtg ggggattgag gccctgtttt ctgatttaaa aacaagaggt ttttcggtaa
     1141 caaaaactca actgaaacaa gtagatcgta ttgaacgact tttgttggtt ctgactattg
     1201 ctctttattg ggctgtgtca acaggtatga tgcctcgaga agctcacgta aattctaaaa
     1261 aaaagagaaa agatctttga cttctttctt taagcggggt cttaggttca ttctcaatgc
     1321 cgctctttcc ctaacaaaaa tccccaaact ctggtttcat ataaaatatg tcaggtgtta
     1381 agaggaattt gaatttagcc tcttttcacc tatactcttt ataaaggaga ttgatagatg
     1441 accgaaaccc ctccccctat tgaacaactt acgcttcgta tgatcgccat gccgcgagac
     1501 acgaatcctg ctggtgatat ttttggcgga tggcttgtct ctataatgg
//
LOCUS       DASWLN010000057         3955 bp    DNA     linear   ENV 20-FEB-2024
DEFINITION  MAG TPA_asm: Candidatus Zixiibacteriota bacterium isolate
            SMAG_U2949 k141_948918, whole genome shotgun sequence.
ACCESSION   DASWLN010000057 DASWLN010000000
VERSION     DASWLN010000057.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37960594
            Sequence Read Archive: SRR10376091
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Candidatus Zixiibacteriota bacterium (soil metagenome)
  ORGANISM  Candidatus Zixiibacteriota bacterium
            Bacteria.
REFERENCE   1  (bases 1 to 3955)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 3955)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (26-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/12/2024 08:28:26
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 1,440
            CDSs (total)                      :: 1,416
            Genes (coding)                    :: 1,412
            CDSs (with protein)               :: 1,412
            Genes (RNA)                       :: 24
            rRNAs                             :: 1 (16S)
            partial rRNAs                     :: 1 (16S)
            tRNAs                             :: 21
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 4
            CDSs (without protein)            :: 4
            Pseudo Genes (ambiguous residues) :: 0 of 4
            Pseudo Genes (frameshifted)       :: 0 of 4
            Pseudo Genes (incomplete)         :: 3 of 4
            Pseudo Genes (internal stop)      :: 1 of 4
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..3955
                     /organism="Candidatus Zixiibacteriota bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_948918"
                     /isolate="SMAG_U2949"
                     /isolation_source="soil"
                     /db_xref="taxon:2053527"
                     /environmental_sample
                     /country="USA: Oregon"
                     /lat_lon="42.99 N 123.63 W"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            complement(<1..470)
                     /locus_tag="VGB16_02030"
     CDS             complement(<1..470)
                     /locus_tag="VGB16_02030"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEX9916490.1"
                     /translation="MVSHKLLLHLCLILITTFIFVTFSLPITLWASVELGKILTFTQS
                     DFAYRNSRSNLFSLSSDNNSPRSNHLLVSKSKSTALRWSLGATLVAPVIGGSFLLKGI
                     SGSEIKAGAVVGIVVGSLGLVFGPSTGHFYAHEYGRAWTGIGLRTLSGVIIMAGA"
     gene            complement(599..680)
                     /locus_tag="VGB16_02035"
     tRNA            complement(599..680)
                     /locus_tag="VGB16_02035"
                     /product="tRNA-Leu"
                     /inference="COORDINATES: profile:tRNAscan-SE:2.0.12"
                     /note="Derived by automated computational analysis using
                     gene prediction method: tRNAscan-SE."
                     /anticodon=(pos:complement(644..646),aa:Leu,seq:gag)
     gene            complement(688..1065)
                     /gene="secG"
                     /locus_tag="VGB16_02040"
     CDS             complement(688..1065)
                     /gene="secG"
                     /locus_tag="VGB16_02040"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011552885.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0005886 - plasma membrane [Evidence IEA];
                     GO_function: GO:0015450 - protein-transporting ATPase
                     activity [Evidence IEA];
                     GO_process: GO:0043952 - protein transport by the Sec
                     complex [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="preprotein translocase subunit SecG"
                     /protein_id="HEX9916491.1"
                     /translation="MFGLMLTLHVIICVALIITILLQSSKGEGLAGAFGGSGITGAVF
                     GGRGAATFLSKATTVLAIAFFVSSLGLSFLVSSIRRTTPEGAIQKQAREERAQPGSGP
                     ATNVPGTNEEQGTKTPVFPEEGK"
     gene            complement(1075..1830)
                     /gene="tpiA"
                     /locus_tag="VGB16_02045"
     CDS             complement(1075..1830)
                     /gene="tpiA"
                     /locus_tag="VGB16_02045"
                     /EC_number="5.3.1.1"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_007290065.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004807 - triose-phosphate isomerase
                     activity [Evidence IEA];
                     GO_process: GO:0006096 - glycolytic process [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="triose-phosphate isomerase"
                     /protein_id="HEX9916492.1"
                     /translation="MRIRIIAGNWKMYKSSEEARILASDLKNRFAKVSGVKIVVCPPF
                     TALPTIYTVIKNSNLQLGAQNMHWEKEGAFTGEVSAEMLLAVGCRYVILGHSERRTYF
                     SEDNQMINKKVKSAVKSGLVPIICVGEKLEEREKGKTKDVVEEHVIGAFQGLSKENAT
                     KTVIAYEPVWAIGTGKTASPKQANEVHIFIRQLLSETYDQKTADNIDILYGGSVKPEN
                     AEELLAQSDIDGALVGGASLDADSFEKIVNSIS"
     gene            complement(1838..3031)
                     /locus_tag="VGB16_02050"
     CDS             complement(1838..3031)
                     /locus_tag="VGB16_02050"
                     /EC_number="2.7.2.3"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_012503296.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0005737 - cytoplasm [Evidence IEA];
                     GO_function: GO:0004618 - phosphoglycerate kinase activity
                     [Evidence IEA];
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_process: GO:0006096 - glycolytic process [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="phosphoglycerate kinase"
                     /protein_id="HEX9916493.1"
                     /translation="MNKLSIEDLSLKDKKVLMRVDFNVPLKNGEVTDDTRIVESLPSI
                     KKVLKEGGRLILMSHLGRPKGKPNSEMSLKPVAKRLEKLLNKPVKFVNDCIGPEVEKT
                     VSELKSGECLLLENLRFYAQEEENDPEFAGKLAKLGEIYINDAFGTAHRAHASTEGVT
                     KYFKQCAAGYLMQKELKYLGMALSNPSRPFVAILGGAKISGKIDVISSLMDKVNTLLI
                     GGGMVFTFYKALGFGIGNSLVEEDKISLAKEILDKAKKKGLDIVLPEDIVIAKEVDEK
                     SEVKIVDKDKIESGWKGLDIGPKTITLFAKKLENAKTVVWNGPMGVFEIERFSKGTIE
                     IAKVLAKITRKGATTIVGGGDSASAIAKAGVEKEISHISTGGGASLEFLEGKTLPGVA
                     ALTDK"
     gene            complement(3173..>3955)
                     /locus_tag="VGB16_02055"
     CDS             complement(3173..>3955)
                     /locus_tag="VGB16_02055"
                     /EC_number="1.2.1.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_009307178.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004365 - glyceraldehyde-3-phosphate
                     dehydrogenase (NAD+) (phosphorylating) activity [Evidence
                     IEA];
                     GO_function: GO:0051287 - NAD binding [Evidence IEA];
                     GO_process: GO:0006006 - glucose metabolic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="type I glyceraldehyde-3-phosphate dehydrogenase"
                     /protein_id="HEX9916494.1"
                     /translation="LEVVSEKDPANLPWRKLGVDIVVESTGKFTDMEGASKHITAGAK
                     KVLISAPGKKVDATIVLGVNEEIYDSKKHNVISIGSCTTNCLGPVAKVLNDNFKIEKG
                     FMTTIHAYTNDQKILDLPHKDLRRARAAAVSIIPTTTGAAKAISLVLPELEGKMDGIA
                     IRVPVADASLVDLACILSKEVTVEQINGAFRKAARNSKLSKYLEYCEDPIVSVDVIGN
                     HHSAVFDSLSTFAKGNFMKVFAWYDNEWGYSCRMVDMLGMMM"
BASE COUNT         1031 a         1008 c          729 g         1187 t
ORIGIN      
        1 gcacccgcca ttataatgac acctgaaagt gttcttagac caatacccgt ccatgctcgt
       61 ccatattcgt gagcataaaa atgtccagtt gagggaccaa atactaaacc gagcgagccg
      121 acaacaatcc caacaactgc ccctgctttg atttcacttc ccgatatccc cttcaacaga
      181 aatgatccgc caatcactgg agccacgaga gttgctccca aagaccacct caaggcagtg
      241 ctcttcgatt tagaaacaag aagatggttg gaccttgggc tattattatc tgaagataga
      301 gagaatagat tggagcgaga attcctataa gcaaaatcgc tctgagtaaa agtgagtatt
      361 ttaccaagct caacgcttgc ccaaagcgta ataggaagag aaaaggtgac aaaaatgaaa
      421 gttgtaatta gaataaggca caagtggaga agaagtttat ggctaaccat actagccccc
      481 ttcggtatta aggtgtattt ccagttacgt caggagtagc tcctgacaga acaaaaagat
      541 tttaaacttg taaactgtta aaccctgcac cgttctggtg cagggtaaac tgatttcctg
      601 cggaaggcgg gacttgaacc cgcatggctt gcgccgccac cccctcaaga tggtgtgtct
      661 gccagttcca ccacttccgc aaaaagatta tttaccttct tctggaaaca ccggagtctt
      721 tgttccttgc tcttcattcg ttccgggaac gtttgtggct ggaccacttc ccggttgagc
      781 cctctcttcg cgtgcttgct tttggatagc tccttcgggt gtggttcttc ttatgctcga
      841 gaccagaaaa ctcaacccta aggaactgac aaaaaaggcg atggctaaga ctgtagtagc
      901 cttcgaaaga aaagtggctg ctccccttcc tccaaaaact gccccggtta tgccgcttcc
      961 accgaaagct ccagctaacc cctccccttt gctggattga agcaatatcg taatgattag
     1021 agcaacgcag ataatcacat gcagagtaag cattaaacca aacaagttaa cctcctatga
     1081 tatgctatta actattttct cgaaagaatc cgcatccaaa cttgccccac ccactaaagc
     1141 tccgtcaatg tccgactgag ccaagagctc ctcggcattt tccggcttaa ctgaaccgcc
     1201 ataaaggata tcaatattgt cggctgtttt ctggtcatat gtttcagata gaagctgacg
     1261 aatgaaaata tgtacctcat ttgcctgctt tggacttgcg gttttcccgg ttccaattgc
     1321 ccataccggc tcataggcaa taactgtctt ggtggcattt tctttggata gaccttgaaa
     1381 ggcaccaatt acgtgctctt caaccacatc tttagttttt cctttttctc tttcttccaa
     1441 tttctctccc acgcagatta taggaacaag cccggacttt actgcggatt tgactttttt
     1501 gtttatcatc tggttatctt cactgaaata tgttctcctt tcggaatgtc ctaggatgac
     1561 gtatctgcac ccaaccgcca aaagcatttc tgctgaaacc tcccctgtga aagcaccttc
     1621 cttttcccag tgcatatttt gagcgcccag ttgcaaattg gaatttttga taacggtata
     1681 gattgttggc aatgcagtga aaggcggaca cacaactatt tttactccac ttactttggc
     1741 aaatctattc ttcaagtccg aagctagaat ccttgcttcc tcactgctct tatacatctt
     1801 ccaattgcca gctattatgc gaattcgcat tttgtattta tttatcagtt aaagctgcca
     1861 caccgggtag ggtttttcct tccaggaact caagcgaagc gcctccgcct gtggagatat
     1921 gcgatatctc tttttccaca cctgccttgg caatggcgga agctgaatcg ccgccgccta
     1981 cgatagtggt agcaccctta cgagtgattt tagccaaaac cttggctatc tctattgttc
     2041 ccttggaaaa cctttctatc tcaaatactc ccattggccc attccataca accgtcttag
     2101 cattttccaa cttcttggca aaaagggtaa tcgtctttgg accaatgtcc aaccctttcc
     2161 atcccgattc aattttatcc ttatccacaa tcttgacttc tgatttctca tccacttcct
     2221 tggctataac gatgtcctcc ggcaagacta tgtccaaacc ttttttcttc gccttatcca
     2281 aaatctcttt tgctaaactt atcttatcct cttcaaccaa ggagtttcct attccaaaac
     2341 ccaaagcttt gtaaaacgtg aagaccattc caccaccaat taggagggta tttactttat
     2401 ccattaaact gcttatcaca tctatcttgc cggagatctt agcaccacct aatatggcaa
     2461 caaaaggacg ggatggattc gacaatgcca ttcccagata cttgagttct ttctgcatca
     2521 aataaccagc tgcacactgt ttgaaatact ttgtaacccc ttcagttgag gcgtgggcac
     2581 gatgtgctgt gccaaaagca tcattgatgt atatttcccc cagcttagct aatttcccag
     2641 caaattcagg gtcattttct tcttcctgtg cgtaaaacct caggttctct aataatagac
     2701 actcacctga tttcagctct gaaaccgtct tttcaacctc tggccctata caatcattta
     2761 caaacttgac tggcttgttt aaaagtttct ctaatctttt ggctactggc ttcaggctca
     2821 tctcgctatt gggttttccc ttgggtctgc ccaaatgaga catcaggata agcctcccac
     2881 cttcttttag aacctttttt attgaaggca aagattccac aatcctcgtg tcatctgtta
     2941 cctccccatt cttcagagga acattgaagt ccaccctcat cagaaccttc ttatctttta
     3001 gacttaaatc ctcaatgctt aatttgttca tatccccctc cgaaaatgtt cttcaatgtt
     3061 tgaagtatta ctttagatat ttaccttaag taattgtcat aggttaactt ccattgttca
     3121 taattcacag ttcgctgttc gctgttaaat catctacata cttaaaccaa aactacatca
     3181 tcataccgag catatccacc attctgcagg aataacccca ctcgttatca taccaggcaa
     3241 acaccttcat aaagttacct ttggcaaagg tggagaggct atcaaatacg gcggagtgat
     3301 ggttgccgat gacgtcaacc gaaacaatcg ggtcttcgca atattccaaa tacttggaaa
     3361 gtttgctgtt tcttgctgct tttctgaaag ccccattgat ctgctcaact gttacttctt
     3421 tcgaaaggat acaggctaag tcaactaaag acgcatctgc cactggaacc cgaatagcta
     3481 ttccatccat ctttccttcc agctcaggca gaaccaagga aattgccttg gctgctccgg
     3541 ttgtcgtggg tattattgag acagcggctg cccttgccct tctcaggtct ttatgcggca
     3601 aatctaatat tttttggtca ttggtgtaag cgtgtattgt ggtcataaaa cctttttcta
     3661 tcttgaaatt gtcatttaag actttcgcca cgggaccgag gcagttggtg gtgcaagagc
     3721 cgatggagat gacattgtgc tttttggaat cataaatctc ttcattcact cccaaaacga
     3781 tggtggcatc cacctttttt ccgggggcgg aaatcaagac tttcttggct ccggctgtta
     3841 tgtgcttcga agctccttcc atatccgtga atttgccggt tgactcaact acgatatcaa
     3901 cacctaattt cctccaagga agattggctg ggtccttttc acttaccacc tccag
//
LOCUS       DASWLN010000058         7334 bp    DNA     linear   ENV 20-FEB-2024
DEFINITION  MAG TPA_asm: Candidatus Zixiibacteriota bacterium isolate
            SMAG_U2949 k141_568578, whole genome shotgun sequence.
ACCESSION   DASWLN010000058 DASWLN010000000
VERSION     DASWLN010000058.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37960594
            Sequence Read Archive: SRR10376091
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Candidatus Zixiibacteriota bacterium (soil metagenome)
  ORGANISM  Candidatus Zixiibacteriota bacterium
            Bacteria.
REFERENCE   1  (bases 1 to 7334)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 7334)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (26-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/12/2024 08:28:26
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 1,440
            CDSs (total)                      :: 1,416
            Genes (coding)                    :: 1,412
            CDSs (with protein)               :: 1,412
            Genes (RNA)                       :: 24
            rRNAs                             :: 1 (16S)
            partial rRNAs                     :: 1 (16S)
            tRNAs                             :: 21
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 4
            CDSs (without protein)            :: 4
            Pseudo Genes (ambiguous residues) :: 0 of 4
            Pseudo Genes (frameshifted)       :: 0 of 4
            Pseudo Genes (incomplete)         :: 3 of 4
            Pseudo Genes (internal stop)      :: 1 of 4
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..7334
                     /organism="Candidatus Zixiibacteriota bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_568578"
                     /isolate="SMAG_U2949"
                     /isolation_source="soil"
                     /db_xref="taxon:2053527"
                     /environmental_sample
                     /country="USA: Oregon"
                     /lat_lon="42.99 N 123.63 W"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            complement(325..1026)
                     /locus_tag="VGB16_02060"
     CDS             complement(325..1026)
                     /locus_tag="VGB16_02060"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_009201999.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="4Fe-4S double cluster binding domain-containing
                     protein"
                     /protein_id="HEX9916495.1"
                     /translation="MTKTDLSFQIQTALLKKGASLVGFADLGDLLPAVRHSKRFAISI
                     AVALDKSAVADIKNGPTKEYHSEYKKVNVLLSNLAKYAAEMIKDLGYEAIPKEPTGVG
                     IDQKTQTTILPHKTVATKAGLGWIGKCALLVTGKYGSAVRLTTVLTDALLETPTPILE
                     SQCGDCTICVDSCPGNAPRGKNWNSDLHRNLFFDVFACEKTARKQGITKVGIDDTICG
                     ICIAVCPWTIRYTQD"
     gene            complement(1044..1523)
                     /locus_tag="VGB16_02065"
     CDS             complement(1044..1523)
                     /locus_tag="VGB16_02065"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_007059332.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="pyridoxamine 5'-phosphate oxidase family
                     protein"
                     /protein_id="HEX9916496.1"
                     /translation="MPKYHMNKKEREITNRNVLMKILKQGKYVTISMCRDNEPYIVTL
                     SYGYDQVKNALYFHCATKGLKLDFVKFNPNVCATIIEDKGYKMGDCSHAYRSVVFWGK
                     IHLIKNLKEKKYGMDVLLNHLEDNPKKLKQRNLKSEEAYRKVGILRLDIEEMKGKQG"
     gene            complement(2334..4391)
                     /locus_tag="VGB16_02070"
     CDS             complement(2334..4391)
                     /locus_tag="VGB16_02070"
                     /inference="COORDINATES: protein motif:HMM:NF012801.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0015267 - channel activity [Evidence IEA];
                     GO_process: GO:0015891 - siderophore transport [Evidence
                     IEA];
                     GO_process: GO:0055085 - transmembrane transport [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="TonB-dependent receptor"
                     /protein_id="HEX9916497.1"
                     /translation="MHKMFLLVIFLLSFSYCLAEETKNKDNLPIYYLEPVIVTATRYP
                     LDLSRIPNHTSFLTKNEIQKRNLLNLGDALKNLAGLDFRTNGVLGQNSTISFRGSFTS
                     QVLVLQDGRALNSLTYGNFNLSDFSVENVERLEIVRGPLSSLYGANALGGVVNIITKD
                     KMENIFSSSLTFGDFNTQIYSFDVSPKLNDFSFLFGVQRKSTDGDRENSDFYSFSTNS
                     KLTYHPSDRFNSHLLVVTQKDTIGIPGVIPDPNKVPPYGDKDVYSLYDKQSDFNWSAD
                     LSSDIVFREKDMLSGKIYLDRRKLNYHTLYDMWDSYYNTVKIVEDDRYITTTYGGFMQ
                     YLYNLKPEKEDKLSFGLDFHQDRLKTTQNLDYQPSGLTDLMDTIIAYNPKTENFACWG
                     NLNIDFKDIMTYQLGARYDHHSLYSGNVSPNFGLIFHLNENGQIKFSYGQAFRAPTFN
                     DLYWPQGGNKNLKPEKGESYEVSLYLNNKDNFFDLTFFSRQVKDLITWAPLGENNLWQ
                     PFNLDKYEAWGIEADAKISLSKYLKLNHNWTLNLGEETKQLLVYSYYDYGTGKISQRF
                     EETKRDAAFTPKLIINLGLEVSTKFGLEASLDLNHLYKRINYYADYSDYPNINYEKKT
                     IPASTNLDFSLNQKMTSKVYLFAKVCNLFDQKNPTRFGNSLYDKDYPNPGRRMNGGVR
                     IEI"
     regulatory      complement(4699..4874)
                     /regulatory_class="riboswitch"
                     /inference="COORDINATES: nucleotide
                     motif:Rfam:14.4:RF00174"
                     /inference="COORDINATES: profile:INFERNAL:1.1.4"
                     /note="cobalamin riboswitch; Derived by automated
                     computational analysis using gene prediction method:
                     cmsearch."
                     /bound_moiety="adenosylcobalamin"
                     /db_xref="RFAM:RF00174"
     gene            4964..5611
                     /locus_tag="VGB16_02075"
     CDS             4964..5611
                     /locus_tag="VGB16_02075"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_012965594.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016787 - hydrolase activity [Evidence
                     IEA];
                     GO_function: GO:0046872 - metal ion binding [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="MBL fold metallo-hydrolase"
                     /protein_id="HEX9916498.1"
                     /translation="MSSQVTFQWFGHSCFKVSTDKLSVVTDPFDKSVGYPVPSCDSDV
                     ITVSHEHFDHNNVSCIKGNPQVLRGKGNIKGLDFSAVETSHDEAGGSKRGKNNIFVWS
                     MEGIKIAHLGDLGHILSSEQVAKIGAVGILFIPVGGYYTISPKEADSIISQLKPKVVI
                     PMHYKTEVMGENFPIVKVSEFTSNKKNVVNVGKNTVTFEKDKLPKETTVYVLEYK"
     gene            5737..6465
                     /locus_tag="VGB16_02080"
     CDS             5737..6465
                     /locus_tag="VGB16_02080"
                     /inference="COORDINATES: protein motif:HMM:NF014670.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="creatininase family protein"
                     /protein_id="HEX9916499.1"
                     /translation="MPVKLEQLDWIEFGKLIPKKMDTVILPIGTVEAHGVISLSTDTL
                     IPLYIAEQIADKFNAIIAPPIHYGITRSMYGFPGSLTVSSETFEKYVTEILFSFADKQ
                     FRKIVIINGHGGQMDELKNAALKVNQERKVKVAVVHWWVLCEDAVQEIYGQTGGHAAL
                     DETAAVLVINEDYVHRKNYKKSLNYVSRKGLSVLPSPQPILLYKEGEGEPDFDVKKAK
                     LYMNKVIQKIEEYLKEVFVGWKKL"
     gene            6521..6823
                     /locus_tag="VGB16_02085"
     CDS             6521..6823
                     /locus_tag="VGB16_02085"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEX9916500.1"
                     /translation="MIEIAIEVERTKKAMLGDYTVDTIASQKWFSGCLGLISRVVGEK
                     DSYNRSFKNLERDIGRLSCLNFGLGILYALREDLKKGLLIRRDFYIAAEIYDDALF"
     gene            6911..7138
                     /locus_tag="VGB16_02090"
     CDS             6911..7138
                     /locus_tag="VGB16_02090"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEX9916501.1"
                     /translation="MKNEIYFNSQENILALNERLYQAELENYNSLIYKRMIYWADLQG
                     KTLQGESDQFDQKESEDMYKWSRFFLEKFLT"
BASE COUNT         2114 a         1479 c         1574 g         2167 t
ORIGIN      
        1 gctgaaaaag ttcggcagag tggtcgggca aggtgcccga cctacgaaaa atcaataatt
       61 tatctcaaga tacggttcga caagggcttc gactgagtcc ttcgacggtg agccactcag
      121 gacggtcgaa ccgctcagcc gaatgtcctc accgtcctga gcaaggtcga aggacagaat
      181 tcgtgtattc ccgtaggtgt gaaccattct gattcacacc gtaggcaggg ctcccagccc
      241 tgcagaaagg ttttttcagt atctcattca ttcagtttga gcaagaattg gaatttccga
      301 accaacgacg attcatattc cctcttagtc ctgagtatat cttatggtcc aggggcaaac
      361 cgcaatgcat attccacaaa tcgtatcatc aattcccact ttggtgatac cttgctttct
      421 tgctgttttc tcgcaagcga aaacatcaaa gaacaaattg cgatgtaagt ctgaattcca
      481 atttttccct cgtggtgcat ttcccggaca gctgtccaca caaattgtgc aatcaccaca
      541 ttgtgactcg agtattgggg ttggggtctc tagtaaggcg tcagttaaaa ctgtagttaa
      601 acgaacagca gagccatatt ttccagtcac tagcaaagca cattttccta tccatcccag
      661 tccggctttg gtggcaactg ttttatgcgg aaggattgtg gtttgggttt tctggtcaat
      721 gcccacaccg gttggctctt ttggtatggc ttcatatccc aagtctttta tcatctcggc
      781 agcatatttc gccaaattcg ataacaaaac gtttaccttt ttatattcgg aatgatactc
      841 cttggttggg ccgtttttaa tatctgcaac tgcggattta tccaaagcca ctgcgattga
      901 gatggcaaat cttttggaat ggcggacggc gggtagtaaa tcacccaaat cggcaaaacc
      961 aaccaaagaa gctccttttt tgagtaaggc agtttgtatt tgaaaagata aatcagtttt
     1021 ggtcatttca gtctgcctga cttctatccc tgtttcccct tcatttcttc tatatccaat
     1081 cttaaaattc ccactttacg gtatgcctct tcggacttga gatttctctg tttgagtttt
     1141 ttgggattat cttctaaatg attcagaagc acatccatcc catatttttt ctcttttagg
     1201 ttttttatta aatgtatctt tccccaaaag acaacggagc gatatgcatg agaacaatcg
     1261 cccattttat atcctttgtc ttctattatt gtggcacata cattgggatt gaatttcaca
     1321 aaatcgagct tcaaaccttt ggttgcacag tgaaaataga gggcgttttt tacttggtcg
     1381 tacccgtagc tcaatgtcac tatgtatggc tcattgtccc gacacataga gatagtaaca
     1441 tattttcctt gtttcagtat cttcatcagt acatttcgat tggttatctc tcgttccttt
     1501 ttgttcatat gatattttgg catgttcttt ccttttggat agtttaatat atcatagcgt
     1561 attttctttg taaagaagaa acagttattc aatacaccag cataatgtca ttacgaatgg
     1621 ttagtttaag agattgcata aattgtgaag tggattgcac atcggaacaa ctaaaaaaag
     1681 gaaaactccc tgacctgtga tccacaattt tagcacaaaa tatatcgtct atgtaatgga
     1741 ttttatgata ataagatagg ggagatattt tttgattcaa agaagaggat gctttacctc
     1801 taatatctaa tgcaaaaacc cctttggcat acaaattgct tgtttctgac aggagaagtt
     1861 aaagcggatg gtaaaaagcg acaggaaacc cattcccaag acacttcttt gttatggtaa
     1921 gcgggagtgg tgcgtcccgc cattaggcgg gacatcccgc catcgggcgg gacatccccc
     1981 ctccaccact cccaactaaa agggcggccc aaatgggtcg ccctttgatt tttttattta
     2041 gtccagtggc ggatgcccgt gggctaccca attataattt taaattattc taagccaaag
     2101 gcagggtcgg atgcctgccc gtctcttccc gctcgacctc cgtggccaga gaatccgacc
     2161 cctacaattg gttttgtttc tcgtaggggt ttgatttatc aaaccctctc ccactccttg
     2221 aaaaggagag gaactgggta agtaatggct gctgccatta cgttttcggg aacggcgcag
     2281 ccgttcccta ccctgcttga tgcaaaattg ttatcaggga tttgatatgt taatcaaatt
     2341 tcaattctca cccccccatt cattcttctt ccgggattgg ggtaatcctt atcgtataga
     2401 ctatttccga atctcgtagg atttttctgg tcaaaaagat tacagacttt tgcgaagaga
     2461 taaactttac ttgtcatctt ttgatttaag ctaaaatcca gatttgtgct ggcaggtatg
     2521 gtttttttct cataatttat atttggatag tctgaatagt cagcataata attaattctc
     2581 ttgtaaaggt gattcagatc caaacttgct tccaatccaa atttcgtgct cacttccaag
     2641 cccaggttga taattagttt tggagtgaaa gcggcatctc ttttggtttc ctcgaagcgc
     2701 tgggatatct tgccagttcc gtaatcgtaa tatgaataaa ccaaaagttg tttggtctct
     2761 tctcccaaat taagagtcca attatgattc aacttaagat acttagatag acttatcttg
     2821 gcatccgcct caattcccca ggcttcgtat ttatccagat taaaaggttg ccagaggttg
     2881 ttttctccaa gaggagccca ggtgattaaa tccttaacct gacgactaaa gaaagtgagg
     2941 tcaaagaaat tatccttatt attcaggtac aaggagactt cgtaactttc acccttttcc
     3001 ggtttcagat ttttattacc gccttgaggc caatataggt cattgaaagt gggagctcgg
     3061 aaagcctgac cgtaagagaa tttgatttga ccattctcat tcagatgaaa aatcaaaccg
     3121 aagtttggac tgacgtttcc agaatataag gagtggtggt cataccttgc tccgagttgg
     3181 taagtcatta tatctttgaa atctatattc aagtttcccc agcaggcaaa gttttcggtt
     3241 ttgggattgt atgctataat tgtgtccatt aaatcggtta agcctgatgg ctgatagtcc
     3301 agattctgtg tggttttgag tcggtcctgg tgaaaatcaa gtccaaagct taacttatcc
     3361 tccttctccg gtttcaggtt gtaaagatac tgcataaatc caccgtaagt tgtggtgata
     3421 tatctgtcat cttcaactat cttcacggtg ttataataag aatcccacat atcgtaaagg
     3481 gtatgataat tcaactttct tctatcaaga taaatcttgc cagagagcat atctttttcc
     3541 ctgaagacaa tatctgagct taaatcagct gaccaattga agtcactttg tttatcatat
     3601 agagaataaa catctttatc tccataggga ggaaccttgt tcggatcagg aatcacgcca
     3661 ggaataccga ttgtgtcctt ctgggttaca actaaaagat gagaattgaa tctgtcagac
     3721 gggtgatagg tcagtttgct gttggttgaa aaggaataaa aatctgaatt ctctctatct
     3781 ccatccgttg attttctctg gactccgaaa agaaatgaaa aatcgttcaa cttgggcgaa
     3841 acatcaaaag agtaaatttg ggtattaaaa tccccgaaag ttagactact ggaaaagata
     3901 ttttccattt tatccttggt gatgatattg actactccac ccaaagcatt tgctccatat
     3961 aagctggaca ggggtcccct tacgatttcc agccgttcaa cgttctccac tgaaaagtcg
     4021 ctcaggttga aattcccata agttaaggaa tttaatgctc tgccatcctg cagcactaaa
     4081 acctgagagg taaatgagcc tctgaaactg atggtggagt tctgtcctaa aactccattt
     4141 gtcctgaaat ccagaccagc taagtttttc agggcatcgc ccaagttcag aagatttctt
     4201 ttttgtattt catttttagt caaaaatgaa gtgtgattgg gaattcggga caggtccaga
     4261 ggatagcggg tggcggtcac aatcaccggc tcgagataat aaatgggcaa attatccttg
     4321 ttcttggtct cctcagccag gcaataggaa aagctcaata agaatataac aagaagaaac
     4381 atcttatgca tacctacctc cgataaagca gttttgcccc gcaccattat ggtgcagggt
     4441 ttgagttttg agttttggga acctcaccct aaccctctcc tttgaaagga gtggggataa
     4501 aaagacagtt taacagttta gaagtttaag aacttgtagg ggttcgattc atcgaacccg
     4561 ttgatttttg ggtcggataa atccgacccc tacaacttca aaattcaacc caacaaaaaa
     4621 cccagtctca agacacagaa ttcgtgccac cttcgaactg ggcaaaattg tgttttaacc
     4681 ttggcctcga aggtcaaaag acattattct caatttggca ggtctcctga cttgtggcat
     4741 cgtgccttgg gctttccttc ccatcccgcc taaagacggg acagtggttt agcaagccct
     4801 cgtagccact tacagtagcg gggctgtggc aaattttcac tgccttccct taaaccaaat
     4861 ttgatatcaa aataaataag ccaatcacaa agtcaagatt tttcttgaaa aaagttgaaa
     4921 aaggttttaa aatgattaaa ttaaggaaaa caaaggaggt taagtgagca gtcaagttac
     4981 tttccaatgg tttgggcata gctgttttaa ggtttcaact gataagcttt cagtggtcac
     5041 cgaccctttt gacaaaagtg tgggataccc tgtgccaagt tgcgattcag atgtcatcac
     5101 cgtcagtcac gagcattttg accataataa cgtctcctgc atcaagggga acccacaagt
     5161 actgagaggg aagggaaata tcaaggggct tgatttcagc gcagtggaga cttcgcacga
     5221 tgaagcaggt ggctccaaac gtggcaagaa taacatcttc gtctggtcaa tggagggaat
     5281 taaaatagcc cacttaggag atttgggtca tatattaagt tctgagcaag ttgccaagat
     5341 tggtgcagtt ggcattcttt ttattccagt aggaggatat tacactatca gcccaaagga
     5401 ggcagattca ataatctctc aattgaaacc gaaagttgta atcccgatgc attacaagac
     5461 cgaagtgatg ggggagaact ttcccattgt caaggtaagc gagtttacgt ccaacaagaa
     5521 aaacgtggta aacgtgggta agaatacggt caccttcgag aaagataaac tacctaagga
     5581 aacgacggtt tacgttttgg agtataagta gtcaagtata accaaagcag agtataacgt
     5641 agggataggt ccaaaggcct atccgaattc gaaattatga aatgcgggtg gacgtggggg
     5701 tccgccccta catataataa atgaaaggag aattgcatgc ccgtaaaact tgaacagctc
     5761 gattggattg aatttggcaa attaatcccc aagaagatgg ataccgtgat tttgccgatt
     5821 ggcacagtgg aggcgcacgg ggtaataagt ctatccaccg atacccttat ccctttgtat
     5881 atcgccgaac agatagcgga taaatttaat gccataattg ctccacctat tcattatggc
     5941 ataacccgca gtatgtatgg ttttcccggc tccctgacgg tttcgtctga aacctttgaa
     6001 aaatacgtga ctgaaattct attctctttt gctgataaac aatttaggaa gatagtgata
     6061 ataaacgggc atggcgggca gatggatgag cttaaaaatg cggctttgaa agttaatcag
     6121 gaaagaaaag tcaaggtcgc agtggttcat tggtgggttt tgtgcgaaga tgccgtgcaa
     6181 gagatttatg gccagactgg tggacacgct gctttagatg aaaccgcagc agttttagtt
     6241 ataaatgaag attatgttca taggaaaaat tataagaaga gcttgaatta cgtttctcga
     6301 aaagggcttt ccgttcttcc ttctccacag ccgattcttc tttacaagga aggggaggga
     6361 gagccggatt tcgacgtcaa gaaagccaag ctttacatga ataaagttat ccagaaaatc
     6421 gaagaatatc taaaagaggt gtttgtaggt tggaaaaaac tgtagaaaaa aagacaatcc
     6481 ctctgctttc gataagaaaa tagaggaaag accgaatgag ttgattgaga tagctattga
     6541 ggtggaaagg accaagaagg cgatgttggg ggattacact gtcgatacca tcgcatccca
     6601 gaaatggttt tccggctgtc tcggtttaat ctcccgtgtg gttggagaaa aggatagtta
     6661 caatcgcagt ttcaagaatc tggaaaggga tattggccgc ctatcctgcc tgaatttcgg
     6721 cttaggaata ctgtatgccc taagggagga tttgaaaaaa gggcttctaa ttcgtcgtga
     6781 tttctacata gcggccgaga tttacgatga tgcgcttttc taggcttatt tcctcttggg
     6841 gaaaaaacaa aaaagcttag cggttctttt gatgggaacg gtattgcatg gtgtcttgag
     6901 aagactttgt atgaaaaatg aaatatattt caatagccag gaaaatatcc tcgccttgaa
     6961 cgagagattg tatcaagctg agctagagaa ttacaattcc ctgatttaca agaggatgat
     7021 atattgggca gaccttcagg ggaaaacgct gcaaggagaa tctgaccaat tcgaccagaa
     7081 ggaatccgag gatatgtata aatggtcaag gttcttcctg gagaagtttt tgacttgatt
     7141 ttaaaatctt tcttacctat ctttaatcaa caaaaataaa ctaatacaaa cgcattgatt
     7201 tacgttcaat aaatctcccc tcgcccctct tttccaaaga ggggaaaaaa gaggtctccc
     7261 cctttgaaaa agggggattt gatggttcga tatgggcttc gactgagtcc ttcgacggtg
     7321 agccgctcag gacg
//
LOCUS       DASWLN010000059         2919 bp    DNA     linear   ENV 20-FEB-2024
DEFINITION  MAG TPA_asm: Candidatus Zixiibacteriota bacterium isolate
            SMAG_U2949 k141_154279, whole genome shotgun sequence.
ACCESSION   DASWLN010000059 DASWLN010000000
VERSION     DASWLN010000059.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37960594
            Sequence Read Archive: SRR10376091
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Candidatus Zixiibacteriota bacterium (soil metagenome)
  ORGANISM  Candidatus Zixiibacteriota bacterium
            Bacteria.
REFERENCE   1  (bases 1 to 2919)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 2919)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (26-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/12/2024 08:28:26
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 1,440
            CDSs (total)                      :: 1,416
            Genes (coding)                    :: 1,412
            CDSs (with protein)               :: 1,412
            Genes (RNA)                       :: 24
            rRNAs                             :: 1 (16S)
            partial rRNAs                     :: 1 (16S)
            tRNAs                             :: 21
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 4
            CDSs (without protein)            :: 4
            Pseudo Genes (ambiguous residues) :: 0 of 4
            Pseudo Genes (frameshifted)       :: 0 of 4
            Pseudo Genes (incomplete)         :: 3 of 4
            Pseudo Genes (internal stop)      :: 1 of 4
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2919
                     /organism="Candidatus Zixiibacteriota bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_154279"
                     /isolate="SMAG_U2949"
                     /isolation_source="soil"
                     /db_xref="taxon:2053527"
                     /environmental_sample
                     /country="USA: Oregon"
                     /lat_lon="42.99 N 123.63 W"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            <1..325
                     /locus_tag="VGB16_02095"
     CDS             <1..325
                     /locus_tag="VGB16_02095"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=2
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEX9916502.1"
                     /translation="YDEIVLIKGFWGIMRKRIPYYQMREVELVYSGKTAFAPDSGSET
                     LLNLAKCILKTNVDKIVKNDKDFMVSISLNNGKTCDFQVEEPQEFVKLVKEKMGKFRV
                     EAKQF"
     gene            662..1450
                     /locus_tag="VGB16_02100"
     CDS             662..1450
                     /locus_tag="VGB16_02100"
                     /inference="COORDINATES: protein motif:HMM:NF014215.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="alpha/beta fold hydrolase"
                     /protein_id="HEX9916503.1"
                     /translation="MNKSIYLLIFTSLALCLSLGWNCTSEKITREEFFFYAVDSTKLE
                     GKLCIPSSNEKLPPLVILLHMFSHSSKDWEGLVPLLTDKGYAVLNLDLRGHGKSTKRG
                     EKTITYLNPEEVNLRLLPLDVNSALDALKSYMGRVDLERVGIIGASIGANAGLIAAVG
                     NPKIKTMVLISPGLNYQDLMTENAAGRLKEVSVLLISNREDSYSLSSCQRLYSAMQLS
                     KKEVKLVQGREHGNELLLKMPELNKQIANWITENLPARGPEQIE"
     gene            complement(1454..1717)
                     /gene="rpsT"
                     /locus_tag="VGB16_02105"
     CDS             complement(1454..1717)
                     /gene="rpsT"
                     /locus_tag="VGB16_02105"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_005354568.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0000312 - plastid small ribosomal subunit
                     [Evidence IEA];
                     GO_component: GO:0022627 - cytosolic small ribosomal
                     subunit [Evidence IEA];
                     GO_function: GO:0003735 - structural constituent of
                     ribosome [Evidence IEA];
                     GO_process: GO:0006412 - translation [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="30S ribosomal protein S20"
                     /protein_id="HEX9916504.1"
                     /translation="MPKTLFAKKRVRQSLKKKIYNQQAKAKIKSLAKKVERSADWNEA
                     IENLKLATEALDKAARLRVIHKNKASRTKSQLAKLANSKKKQG"
     gene            2210..>2919
                     /locus_tag="VGB16_02110"
     CDS             2210..>2919
                     /locus_tag="VGB16_02110"
                     /inference="COORDINATES: protein motif:HMM:NF015013.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="lipid II flippase MurJ"
                     /protein_id="HEX9916505.1"
                     /translation="MSNSDKIAKSTGIVSVATFASRIFGLIREQVFAYLFGAGFATDA
                     FVAAFRIPNLLRDLFAEGALSSAFIPTFTEHLTNKSKEDAFRLASLVINLLLITLSII
                     IILGIVFAPYIVKVIAPGFEKLAGKQDLTTLLTRIMFPFLLLIALAAVCMGVLNSLGK
                     FGIPAVAPVMLNLGMRLSGFFICPFFHPPIIGMAIGALLGGLGQLLIQVPTLFKLGFK
                     YKLVLDVFNPGVKRILLL"
BASE COUNT          772 a          578 c          690 g          879 t
ORIGIN      
        1 ctatgatgag attgtcttga ttaagggttt ttgggggatt atgcgaaaaa ggatacctta
       61 ctatcagatg agggaggtgg agctggttta ttccggtaaa acagcttttg cgccagattc
      121 aggttcggag acactactta acctggcaaa atgcattttg aaaacgaatg tagataaaat
      181 cgtcaagaat gacaaagatt ttatggtaag cataagtttg aataatggta agacttgtga
      241 ttttcaggta gaggagccac aggagtttgt gaagttggta aaagagaaga tggggaagtt
      301 tagggttgag gcaaagcagt tttgagaacc tcaagcccct cttttcttag gaaagagaga
      361 agttttagag ccgaagcaat taagcccgta tgggacaaga taagctcttg catccacggg
      421 aatatttaat ctatcgcaca aatgtggcaa ccacagcgag ttcgtataaa aacccttgac
      481 aagaggaatt gctcaggcct tattataaga taggagattt tgtcagaaaa agccctctca
      541 ccgctgactt attcatgtca agagaatagc tttattctcc ttcgggagct tgcgagccgt
      601 gctcacttct gtgaactcaa agtacgcgat tcttctgaaa gcaaaacaac agtgtagtca
      661 aatgaacaag agtatatact tattgatatt tacttctctg gctttgtgcc tttctctggg
      721 ttggaactgt acgagtgaga agatcacccg cgaagagttc tttttctatg cggtagatag
      781 caccaaacta gaggggaagc tatgtattcc ttcctccaat gaaaaacttc cccccttggt
      841 tatccttttg catatgttct ctcatagctc caaagactgg gaaggccttg tgccactttt
      901 gacggataag ggttatgcag ttttgaattt agaccttcgg ggacatggga agagcaccaa
      961 aagaggagag aagaccataa cttatcttaa tccggaggag gtgaatctta gacttttgcc
     1021 tttggacgtg aactcagctt tagatgccct gaagagttat atgggaagag tggatttgga
     1081 gagagtggga ataattggtg cgagcatcgg agcaaatgca gggttgatag cggctgtggg
     1141 aaatccaaaa atcaaaacta tggttttaat ctctccgggt ttaaactatc aggatctcat
     1201 gaccgagaac gcagctggaa ggttgaaaga ggtaagcgtc cttttaattt ccaaccgaga
     1261 agacagttat tccttgtcca gttgtcaaag gctctattcc gccatgcaac tatccaagaa
     1321 agaggttaag ctggtgcagg gaagggaaca cggaaatgag ctgcttttga agatgccgga
     1381 gctgaataaa cagatcgcta attggattac agagaaccta cctgccagag gacctgagca
     1441 aatagaataa ttcctaacct tgtttcttct tggaattagc caattttgcc agttgagatt
     1501 tggtgcgact ggctttgttt ttgtgaataa ctcttagccg tgcagctttg tccagtgctt
     1561 cggtcgccaa tttgagattt tctatggctt cgttccagtc ggctgatctc tccactttct
     1621 tggctaaact ttttattttg gctttcgctt gttgattgta tatctttttt ttcagacttt
     1681 gcctgactct tttcttggca aacaaggttt taggcaactt atctcctttt tgtgaaaatt
     1741 gttctttgat tttgtgtgga aataaatata ataccataga aatttttgtc aaacaataaa
     1801 tcagtttcga gtttgcagtt ttgagtttcg agaacctcac cctaaccctc tccttagaaa
     1861 ggagaggatg ttgcaaaatc caagatggtc aggggatggt tgtttggtgc gtcattgcga
     1921 ggagtccagc caagggcgag acgacaaagc aatctgaccc aaatgggtca tcccgaaccc
     1981 gttcgtttca ctcccttcgg gtctgagcct cagggtcgaa gacagggcag gctccgtgag
     2041 ggatctcctg taggatgaga tccttccccc gtcaaggcgg ggtcaggatg acgattgctc
     2101 ttgagattgc ttcgccctac gggctcgcaa tgacaacggc aaataaagtt gctttattct
     2161 tcgtaggagc tcgcaatgac aattggaacg ataatgtgga ataaagatta tgtctaattc
     2221 ggataaaatt gccaaatcaa ccggcattgt ttcagttgcc actttcgctt cacgtatttt
     2281 tggcctaata cgggagcagg tattcgctta tctttttggg gcgggatttg ccacggatgc
     2341 ttttgttgcc gcttttcgca ttcccaatct tctgcgcgac ctttttgcgg aaggggcact
     2401 ctcttccgcc ttcattccga ctttcactga acatttgacc aataaaagca aagaagatgc
     2461 tttccggctc gcctcattag tcatcaatct acttctaata acgctcagca tcattataat
     2521 ccttggcata gtttttgcgc cttacattgt caaagtgatt gctccgggat ttgagaaact
     2581 tgctggaaag caagacctga ccacgctctt gaccagaata atgttcccct ttttactctt
     2641 gatagctctg gctgcggtct gtatgggagt cttaaactct cttggaaaat tcggcattcc
     2701 agctgttgct ccagtgatgc taaatttagg gatgagactc tccgggtttt tcatctgtcc
     2761 attttttcat ccacccatta tcggaatggc aattggggcg cttttgggag gattaggtca
     2821 gcttctcatt caagtcccta cgttgtttaa attgggattc aaatacaaat tggttttgga
     2881 tgttttcaat ccaggagtga agaggatttt gcttctgat
//
LOCUS       DASWLN010000060         3867 bp    DNA     linear   ENV 20-FEB-2024
DEFINITION  MAG TPA_asm: Candidatus Zixiibacteriota bacterium isolate
            SMAG_U2949 k141_569018, whole genome shotgun sequence.
ACCESSION   DASWLN010000060 DASWLN010000000
VERSION     DASWLN010000060.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37960594
            Sequence Read Archive: SRR10376091
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Candidatus Zixiibacteriota bacterium (soil metagenome)
  ORGANISM  Candidatus Zixiibacteriota bacterium
            Bacteria.
REFERENCE   1  (bases 1 to 3867)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 3867)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (26-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/12/2024 08:28:26
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 1,440
            CDSs (total)                      :: 1,416
            Genes (coding)                    :: 1,412
            CDSs (with protein)               :: 1,412
            Genes (RNA)                       :: 24
            rRNAs                             :: 1 (16S)
            partial rRNAs                     :: 1 (16S)
            tRNAs                             :: 21
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 4
            CDSs (without protein)            :: 4
            Pseudo Genes (ambiguous residues) :: 0 of 4
            Pseudo Genes (frameshifted)       :: 0 of 4
            Pseudo Genes (incomplete)         :: 3 of 4
            Pseudo Genes (internal stop)      :: 1 of 4
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..3867
                     /organism="Candidatus Zixiibacteriota bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_569018"
                     /isolate="SMAG_U2949"
                     /isolation_source="soil"
                     /db_xref="taxon:2053527"
                     /environmental_sample
                     /country="USA: Oregon"
                     /lat_lon="42.99 N 123.63 W"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            487..999
                     /locus_tag="VGB16_02115"
     CDS             487..999
                     /locus_tag="VGB16_02115"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_012900592.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="nitroreductase family protein"
                     /protein_id="HEX9916506.1"
                     /translation="MNVFEAIKKRKSIRGYLDKPIEEEKLKRVLEAGRLAPSAKNAQD
                     WKYVVVKDPKLRKELAKACHNQMFIAEAPVVIVGCAIIPDFKLSCGQLAGPIDVTISM
                     DHMTLVAVEEGLGTCWIGAFEEEKVKKVLGIPDWVRIIEILPLGYPAVEGSFRGRRNF
                     EEVFVWDEWK"
     gene            1362..2195
                     /locus_tag="VGB16_02120"
     CDS             1362..2195
                     /locus_tag="VGB16_02120"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEX9916507.1"
                     /translation="MKTKVILFCLVFIFIMAGSSLAQYQPPPEEQKHDVTSPRVTKIS
                     GLSTLSPRKLVDCPTAALLPRGSFDFDVRFFPNGGVNIALGIGLMKRLNVGMSYGGEE
                     IIGDKDPNWNPRIEFAVKYRLINESYMMPAFALGFDSQGYGAYDDSLDRYANKSKGFY
                     GVISKNYLVFEQVPVGLHFGGNYSLENEDKDKSLDLYLGGDVRITEDLAGALEYDLAL
                     NDNRKDQYYGRGYGYLNLGIQWIFQDRLVLELDLKNVLRNKPGDITMSREVRIVYVEY
                     F"
     gene            2290..2424
                     /locus_tag="VGB16_02125"
     CDS             2290..2424
                     /locus_tag="VGB16_02125"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEX9916508.1"
                     /translation="MPELQFTIIALAMLVLALVVMNLNRKVDNVSKDLDLLRRKTGKK
                     "
     gene            2624..3196
                     /locus_tag="VGB16_02130"
     CDS             2624..3196
                     /locus_tag="VGB16_02130"
                     /inference="COORDINATES: protein motif:HMM:TIGR02937.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003677 - DNA binding [Evidence IEA];
                     GO_function: GO:0003700 - DNA-binding transcription factor
                     activity [Evidence IEA];
                     GO_function: GO:0016987 - sigma factor activity [Evidence
                     IEA];
                     GO_process: GO:0006352 - DNA-templated transcription
                     initiation [Evidence IEA];
                     GO_process: GO:0006355 - regulation of DNA-templated
                     transcription [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="sigma-70 family RNA polymerase sigma factor"
                     /protein_id="HEX9916509.1"
                     /translation="MIQAAVTDRELMEKIRQRDDSAFEELVNRYKKKLFNLIYRMLND
                     RNETEDILQETFLRVYRERESYDPHFCFSTWVYTIALNLTKNELKKRSRFKFLELSII
                     QNDRIYSREDEQVDSGLKNLLENTMNKLPTKYKAALVMREVNQLSYEEMSDILKVPLG
                     TIKSRVNRARNMLQKKLKPKLMVNYEVSKA"
     gene            3177..3794
                     /locus_tag="VGB16_02135"
     CDS             3177..3794
                     /locus_tag="VGB16_02135"
                     /inference="COORDINATES: protein motif:HMM:NF024881.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="zf-HC2 domain-containing protein"
                     /protein_id="HEX9916510.1"
                     /translation="MKCRKLKSSIPKYHDGDLTSDERDKLSQHIKECLTCKKEFYQMD
                     KLKELLSNMDALEPSADFNAKLWARIQKEKYNEGYEKAPELSLTFTQKVRWALATCLV
                     TIAMLSVIVLTDPRFSLLVKEEPKSTQTIARSTKHQILVEPDKIKYVMDNYKPLPRKA
                     EVDNPGKTKEYDYILERKAYPQLITTGEQYVLPVVSTRSLIEESY"
BASE COUNT         1225 a          665 c          913 g         1064 t
ORIGIN      
        1 gaaaaagggg gatttgatgg ttcgatatgg gcttcgactg agtccttcga cggtgagccg
       61 ctcaggacgg tcgaaccgct cagccgaatg tcctcaccat ctcttcgagc ctgagccttc
      121 cttcgacact gaggctcagg acgaagtgca gagccgaagg cctgagcaaa gtcgaaggga
      181 gggggatttt aaaatagatt gcattttgcc ctaatgtgta acaacattta atgcgtttac
      241 tatatattca cacaggaata gctcctgacg caacactcaa accaattttt tggagtataa
      301 agctttagct ttatcgtttt tagtggacct aaaggttcac actccaaaaa gaaatttgtt
      361 tttgtagtgg ttggatttat tcgacaagaa tatgtttgat aaatcaaacc actacattct
      421 cgaaactcaa aactcaaacc ctgcaccata atggtgcagg gcaaaactaa cttttcggag
      481 gcaagtatga atgtctttga agcaatcaag aaaagaaaaa gcattcgtgg atatctggac
      541 aagccaattg aggaagaaaa gctgaagaga gttttagaag cgggacgctt agctccttcg
      601 gccaaaaatg ctcaagattg gaaatatgtc gtggtcaaag acccgaaatt aagaaaagaa
      661 cttgccaaag cctgtcacaa tcagatgttt attgccgaag caccggtggt gattgttggt
      721 tgtgccataa tacccgattt caaactctcc tgtgggcagt tggcaggtcc gattgacgtt
      781 accatttcaa tggaccatat gactctggtg gcagtggagg aagggttggg aacatgctgg
      841 attggagctt ttgaggagga gaaagtgaaa aaagttttgg ggattccgga ttgggtgaga
      901 ataatcgaaa tcctacctct gggttatcca gcagttgagg ggtcattcag agggaggaga
      961 aatttcgagg aggtttttgt ttgggatgag tggaaataaa ataaattcaa aattaaaaaa
     1021 gaaaaattaa aaattgggcc agtaaaccta ccttgcctca aggtggggtt gctttgcttt
     1081 ttagacccac gttgggggca acgtgggttt actacttttc cttgagtttt ctcggtggta
     1141 gatgtcactg ggttagccct ataagttcga aattaaaaaa gaaaaattaa aaattgaaaa
     1201 tgaaaggcgg ttatttaagg gccgcccttt attttttatg aaacataata accgatttcc
     1261 gagtaaaact gattagataa ttccttattt cccatctttt ttgttgacta aatcattcga
     1321 ggtgtttaga ttatgttcaa atccaaaacg gaggggaaag aatgaaaacg aaagttatcc
     1381 ttttttgttt agtttttata tttattatgg ctggtagctc cttggctcaa tatcagcctc
     1441 cgcctgaaga gcaaaagcat gatgttacct cacctagagt tacaaagata tccggattgt
     1501 ccactctttc tccgcgcaaa ttagtcgatt gccccacggc cgcgctcctt ccacgaggaa
     1561 gtttcgattt cgatgttcgg tttttcccca atggaggggt gaacattgcc ttgggaattg
     1621 ggctaatgaa aagattgaat gtgggtatgt cctatggagg agaagagatt attggcgata
     1681 aagaccccaa ctggaatccg agaatagagt ttgcagtgaa ataccgtctc atcaatgaaa
     1741 gttatatgat gcccgctttc gctctcggat ttgattcgca aggatatggt gcttatgacg
     1801 attctttgga tagatacgct aacaaatcca aaggattcta tggggtgata agcaaaaact
     1861 acctggtgtt cgagcaagtt ccagtgggtc tgcatttcgg tggtaattat agcttagaaa
     1921 atgaagataa agataagagc ctggatttgt atttaggcgg agatgtcaga atcaccgaag
     1981 acctcgccgg ggctctggaa tacgacttag ctttgaatga taaccgcaaa gaccagtatt
     2041 atggcagagg atatggatac ctcaacttag ggattcagtg gatatttcag gacagattgg
     2101 tcttggagct ggacctgaag aacgttttga gaaacaaacc cggggatatc actatgtcac
     2161 gggaagtgag gatagtttac gtggaatatt tctaacagaa gatagaaata ttgtttattg
     2221 gggcggtttt gttgaaccgc ccttttttta tttaagattt gaagatttaa ggtcgataac
     2281 ataaaagaaa tgcctgagtt acaatttacc ataatcgctt tggcaatgtt ggttctggct
     2341 ttggtagtaa tgaaccttaa ccgcaaggtg gataacgttt ccaaagattt ggaccttttg
     2401 aggaggaaaa cgggaaagaa gtagaaaggc ctgttaataa tagttaatat ttgaggcgac
     2461 ctgtggagtc gcctttttgt ttccggcaca atccaaattg ataaaacctt tcacgcgttt
     2521 catacgtagt aatagttatt gaaacctaaa aaatgcaact aacgaagcat atccttgtaa
     2581 aaataaaatg agcaagggga aggcctcttt ttggggctaa cttatgattc aggcggcggt
     2641 aactgacaga gagctgatgg agaagatcag acaaagggat gactctgcct tcgaggagct
     2701 ggtcaatcgt tataagaaaa agctctttaa cctaatctat cgtatgctaa atgatagaaa
     2761 tgagacggag gatatcctgc aggagacctt cttgagggtt tatcgggaaa gagaaagtta
     2821 cgaccctcat ttctgttttt ccacctgggt ttataccatt gccctgaatt tgacaaaaaa
     2881 tgaattaaaa aagaggagcc ggttcaaatt tttggaactt agtataatcc agaatgatag
     2941 aatatatagt agagaagatg agcaagtaga ttccggtttg aaaaacttat tggaaaatac
     3001 aatgaataaa ttaccgacca aatacaaagc cgctctggtg atgagagagg taaatcagct
     3061 ctcctatgag gagatgtcag atatattgaa agtccccttg gggactataa aatccagagt
     3121 taaccgagcg cgaaatatgt tacagaagaa gttaaagcca aagcttatgg tgaattatga
     3181 agtgtcgaaa gcttaaatcc tctattccaa aatatcatga tggagacttg acctctgatg
     3241 aaagggataa gctgagtcag catatcaaag aatgtctcac ttgcaaaaaa gaattttatc
     3301 aaatggataa attgaaagag ttgttgtcca acatggatgc cttggagcca agtgcagatt
     3361 ttaatgctaa gctttgggca aggattcaaa aggaaaagta caatgaagga tatgagaagg
     3421 ccccagagtt gagtttaaca tttacgcaaa aggtcagatg ggctttggca acctgcttag
     3481 ttaccatcgc gatgctgtcc gttattgtcc tgactgatcc gcgcttcagc ttgttagtaa
     3541 aagaggagcc aaaatctacc caaacaatag cccgatcgac aaaacatcaa atcttagttg
     3601 agccagacaa gatcaagtat gttatggata actataaacc gttgcccagg aaagcggagg
     3661 tggataatcc cggaaaaact aaagaatacg attatatcct ggagagaaag gcttatcccc
     3721 agcttataac caccggagaa caatacgtgc ttccagtggt atcaactaga agtctaattg
     3781 aggagagtta ttaaactaag gagagagtat gaaaaaaaca tatctaatgg tctttttgac
     3841 agtaattgtt ttgtttgtcc tggcaag
//
LOCUS       DASWLN010000061         1941 bp    DNA     linear   ENV 20-FEB-2024
DEFINITION  MAG TPA_asm: Candidatus Zixiibacteriota bacterium isolate
            SMAG_U2949 k141_569890, whole genome shotgun sequence.
ACCESSION   DASWLN010000061 DASWLN010000000
VERSION     DASWLN010000061.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37960594
            Sequence Read Archive: SRR10376091
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Candidatus Zixiibacteriota bacterium (soil metagenome)
  ORGANISM  Candidatus Zixiibacteriota bacterium
            Bacteria.
REFERENCE   1  (bases 1 to 1941)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 1941)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (26-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/12/2024 08:28:26
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 1,440
            CDSs (total)                      :: 1,416
            Genes (coding)                    :: 1,412
            CDSs (with protein)               :: 1,412
            Genes (RNA)                       :: 24
            rRNAs                             :: 1 (16S)
            partial rRNAs                     :: 1 (16S)
            tRNAs                             :: 21
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 4
            CDSs (without protein)            :: 4
            Pseudo Genes (ambiguous residues) :: 0 of 4
            Pseudo Genes (frameshifted)       :: 0 of 4
            Pseudo Genes (incomplete)         :: 3 of 4
            Pseudo Genes (internal stop)      :: 1 of 4
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..1941
                     /organism="Candidatus Zixiibacteriota bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_569890"
                     /isolate="SMAG_U2949"
                     /isolation_source="soil"
                     /db_xref="taxon:2053527"
                     /environmental_sample
                     /country="USA: Oregon"
                     /lat_lon="42.99 N 123.63 W"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            <1..232
                     /locus_tag="VGB16_02140"
     CDS             <1..232
                     /locus_tag="VGB16_02140"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=2
                     /transl_table=11
                     /product="lipoate--protein ligase family protein"
                     /protein_id="HEX9916511.1"
                     /translation="RTVQEVEKKTLMEIDISGDFTMLPKEELSDLEGALKETEREEKK
                     LESKIEEFYEKSGVQTPGVEPQDITKAIQAGQ"
     gene            complement(291..788)
                     /locus_tag="VGB16_02145"
     CDS             complement(291..788)
                     /locus_tag="VGB16_02145"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEX9916512.1"
                     /translation="MVMILSQAWRAIPIFLIVGVPFIPAAEAQQPFDITYCATSNMTT
                     VSASEGLIVLSLDTKGIAMSNHENKVFDNMTFLCVAVGKVVAGKPIGTGYCKYMDPDG
                     DIIVWELSIDVPVDTLKAIQGTGKWKGIKAEGKAALITKAKSITPDTRQVCRRFTGTF
                     ELPKK"
     gene            complement(865..1377)
                     /locus_tag="VGB16_02150"
     CDS             complement(865..1377)
                     /locus_tag="VGB16_02150"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEX9916513.1"
                     /translation="MKKSSLILVLNLILIAAFGLDVQAQPKEATESVTTTWYVTTKVV
                     PLGADRIYLVYEAVGVNVTDTGEGLFHGATARALGGMTIEKGFYNDEYGCGVWNLQNG
                     DKVFINYKFAGKAGTGGTAKGTFTITGGTGKCAGIQGRSDESTRTMVQSALEGVGQSF
                     SKAKFQYKLP"
     gene            complement(1522..1746)
                     /locus_tag="VGB16_02155"
     CDS             complement(1522..1746)
                     /locus_tag="VGB16_02155"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEX9916514.1"
                     /translation="MHLATYIVRIYRSKKGKSRGLVGVVEQIGVKGRKAFTDCDELWE
                     IFCPLKNVRIHKKRENTIISRDKANPWSKL"
BASE COUNT          546 a          444 c          421 g          530 t
ORIGIN      
        1 cagaaccgta caggaagtgg agaagaaaac gttaatggag atagatatct ctggtgactt
       61 caccatgttg ccgaaggagg aactctcaga tctcgaaggt gcattgaagg aaacagagag
      121 ggaagaaaag aagctcgaat caaagatcga agaattctac gaaaagtcag gggtgcagac
      181 ccctggcgtg gaaccccagg acatcacaaa agccatccaa gcaggacaat aggaagcagg
      241 ggatacctcc gaaatttgtt aaaaatatcg gagatgtccc ctatttctct ttatttcttt
      301 ggcagttcaa acgttccagt aaatctgcga cagacttgac gggtgtctgg cgttatcgat
      361 ttagctttgg taatcagtgc tgctttgcct tcggccttaa tgcccttcca tttaccggta
      421 ccttgaatgg cttttaaggt atcaaccggg acatcaatgg aaagctccca aacaataata
      481 tccccatcgg gatccatgta tttgcaataa ccggttccga tgggcttccc tgcaacaacc
      541 tttccaacag cgacgcaaag gaacgtcatg ttatcaaaaa ccttattctc gtggttgctc
      601 atagcaatgc cctttgtatc cagacttaaa actatcaatc cttcgcttgc tgaaaccgtt
      661 gtcatgttcg aagtggcaca gtaggtgata tcaaaaggtt gctgcgcttc ggcagccgga
      721 atgaatggaa ccccaacaat cagaaagatt ggtatggctc tccatgcttg agataagatc
      781 ataaccaaac tcctttctcc cctgtaaaga gggtaggatt aatttttgaa tcttgaaatg
      841 tagcccgtcg ccttatcact aatttcaagg cagcttatat tggaattttg cttttgaaaa
      901 actctgaccc actccctcca aggctgattg aaccatggtt cgggtgcttt catcactcct
      961 gccctgtatg ccagcgcatt tgccagttcc gccggtgatt gtaaaagtcc ccttcgcggt
     1021 tccacctgtt cctgccttgc ctgcgaattt atagttaata aacaccttat ccccgttttg
     1081 gagattccaa acaccgcaac catactcatc attgtagaat cctttttcga tggtcattcc
     1141 gccgagagca cgtgctgtcg cgccgtgaaa gaggccctcc cctgtgtcgg tgacgtttac
     1201 accaacggct tcgtaaacta ggtaaatcct atccgctcct aaaggaacga cttttgttgt
     1261 aacgtaccag gtggtagtga ctgattcggt cgcttctttg ggctgtgcct ggacatccaa
     1321 tccaaaagca gctattagaa ttaagttcaa gactaaaatc aaactagatt tcttcatggt
     1381 ctgtctcctt tcctcctctg aatatcagag cagaattgta agtgtttgct tattaattca
     1441 ggatagatca aagtgggagg ctccaggcga acgaataatc ctctggagcc tcccgtatgg
     1501 gcatcttctg cagactccct ttcataactt gctccatgga ttagctttat ctcttgatat
     1561 aatagtattt tctctctttt tatgaatcct gacgtttttc aaagggcaga aaatctccca
     1621 gagttcatcg caatctgtaa atgcttttct tccttttacc ccaatttgct ccacgactcc
     1681 cacaagacct cgggacttcc ccttttttga acggtaaatg cggacgatgt aggtggctaa
     1741 atgcataact gaaaaaactg cagaataacg gattcttgag caccacccat accatgaggg
     1801 agattacaga ttaggtacag attcgttgga agcagaataa actttggagt taggagattg
     1861 cagtttggag aaacgggaaa aaaggaagaa taaaaaagag atggggggag aggaatatcg
     1921 aaatagatag aaaagactta c
//
LOCUS       DASWLN010000062         1527 bp    DNA     linear   ENV 20-FEB-2024
DEFINITION  MAG TPA_asm: Candidatus Zixiibacteriota bacterium isolate
            SMAG_U2949 k141_314191, whole genome shotgun sequence.
ACCESSION   DASWLN010000062 DASWLN010000000
VERSION     DASWLN010000062.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37960594
            Sequence Read Archive: SRR10376091
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Candidatus Zixiibacteriota bacterium (soil metagenome)
  ORGANISM  Candidatus Zixiibacteriota bacterium
            Bacteria.
REFERENCE   1  (bases 1 to 1527)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 1527)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (26-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/12/2024 08:28:26
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 1,440
            CDSs (total)                      :: 1,416
            Genes (coding)                    :: 1,412
            CDSs (with protein)               :: 1,412
            Genes (RNA)                       :: 24
            rRNAs                             :: 1 (16S)
            partial rRNAs                     :: 1 (16S)
            tRNAs                             :: 21
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 4
            CDSs (without protein)            :: 4
            Pseudo Genes (ambiguous residues) :: 0 of 4
            Pseudo Genes (frameshifted)       :: 0 of 4
            Pseudo Genes (incomplete)         :: 3 of 4
            Pseudo Genes (internal stop)      :: 1 of 4
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..1527
                     /organism="Candidatus Zixiibacteriota bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_314191"
                     /isolate="SMAG_U2949"
                     /isolation_source="soil"
                     /db_xref="taxon:2053527"
                     /environmental_sample
                     /country="USA: Oregon"
                     /lat_lon="42.99 N 123.63 W"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            complement(<1..581)
                     /locus_tag="VGB16_02160"
     CDS             complement(<1..581)
                     /locus_tag="VGB16_02160"
                     /inference="COORDINATES: protein motif:HMM:NF024581.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0046872 - metal ion binding [Evidence
                     IEA];
                     GO_function: GO:0051536 - iron-sulfur cluster binding
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="(Fe-S)-binding protein"
                     /protein_id="HEX9916515.1"
                     /translation="MKETIKKTRAYSCLDCGKCTATCPISRVNKGYSPRRLLTKALIS
                     SNENLLNDSTLWTCLTCMMCQQRCPSDIDYVTFTKLVRAQANSEGQDAKCSHGGALQS
                     LMKIMTAGELKQNRLGWITSDLKTKEKSEYLYFVGCLPYFDVFFSDIKANSLDSARST
                     LKILNFFGIEPQVLANERCCGHDLLWMGDVENF"
     gene            complement(620..862)
                     /locus_tag="VGB16_02165"
     CDS             complement(620..862)
                     /locus_tag="VGB16_02165"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_015590998.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF2283 domain-containing protein"
                     /protein_id="HEX9916516.1"
                     /translation="MAKRKLTQKDFSEIFKAIPHLIKLPARKIWVDYDKETDVLYISL
                     QRPQNATDSEMQEDGVLLRYKDKDLVGITIFEASKR"
     gene            complement(844..1146)
                     /locus_tag="VGB16_02170"
     CDS             complement(844..1146)
                     /locus_tag="VGB16_02170"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEX9916517.1"
                     /translation="MDVANSVNGVPIRLTEKRWFHVVENHDDLAGHYDDVLNTLENPD
                     FVLRGYKSALIAIKGIAKGRYLAVVYKELKRDDGFVLSAYITTKIRKRLIIWRKEN"
     gene            complement(1158..1490)
                     /locus_tag="VGB16_02175"
     CDS             complement(1158..1490)
                     /locus_tag="VGB16_02175"
                     /inference="COORDINATES: protein motif:HMM:NF014692.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_except=(pos:complement(1410..1412),aa:Sec)
                     /transl_table=11
                     /product="hydrogenase iron-sulfur subunit"
                     /protein_id="HEX9916518.1"
                     /translation="MCSGRISPAHVFRAFEKGADGVMWSGUHFGDCHYTFGNHRAAEQ
                     FEITKQLIRLLGIEEKRLRLEWFSAAEGQRFAQVIKQFVEDVKKVGPSPLRNLNKKSS
                     LESANLNI"
BASE COUNT          399 a          362 c          280 g          486 t
ORIGIN      
        1 ttgaagttct ccacatcccc catccacaaa agatcatgcc cgcaacatct ctcatttgcc
       61 aagacctgtg gctcaattcc gaaaaagttc aaaatcttca aagtcgaccg ggcggaatct
      121 aaagaatttg ctttgatgtc agagaagaag acatcgaaat agggaaggca acctacgaaa
      181 tacaagtatt ccgatttttc tttagttttc agatcagagg ttatccatcc caatctattt
      241 tgtttcagct cacctgcggt cattattttc ataagggatt gcaaagctcc cccatgcgaa
      301 catttggcat cctgaccttc ggaatttgct tgggctctca ctaatttggt gaatgtaaca
      361 taatctatgt ccgaagggca tctctgttgg cacatcatgc aggtcaggca agtccacaga
      421 gtgctgtcgt taagaaggtt ctcattcgag ctgattaagg ctttggtcaa aagccttcgg
      481 ggagagtaac ctttattaac tcgggaaatg gggcaggtgg cagtgcactt gccacaatcc
      541 aaacaggaat aagctctggt tttttttatg gtttccttca tcatgttatc tagggtttga
      601 gtttatcaat ctcagatttt tatcttttgg aagcctcaaa aattgtgatt cccaccaagt
      661 ccttgtcctt atatctgagc aacacgccat cttcttgcat ttcactatca gtagcattct
      721 gaggtctttg aagactgata tacaggacat ctgtttcctt atcatagtct acccaaatct
      781 tcctagcggg gagttttatc agatgaggga tagctttgaa aatctcactg aaatcctttt
      841 gagtcagttt tcttttcgcc atattattaa ccttttccta atctttgtgg taatatacgc
      901 tgaaagaaca aaaccatcat ctcttttcaa ttctttgtat actactgcca ggtagcgtcc
      961 tttggctatt ccctttatag cgattagtgc gcttttataa cccctaagca caaaatctgg
     1021 attctcaagg gtgttaagaa catcatcgta atgaccggct aaatcatcat ggttttctac
     1081 aacatgaaac catctttttt ctgttaatcg aatgggaaca ccattcacag aattcgctac
     1141 atccaattgt cttttcctta tatattgaga tttgcactct ccaaagatga tttcttattc
     1201 aaatttctca acggacttgg tccaactttt ttcacatcct ctacgaactg ttttatcacc
     1261 tgggcgaacc tttgtccttc agcggctgag aaccactcca atctcagcct tttttcttct
     1321 atacccagta acctgatcag ttgtttggtg atctcgaact gctccgcagc tcgatgattg
     1381 ccaaaggtgt aatggcaatc gccgaaatgt caccccgacc acatcacgcc gtctgcgcct
     1441 ttttcaaatg cccggaatac atgtgccggg ctgatcctgc cggaacacat cacccttatc
     1501 aatctcacat tgggcggata ttgtatc
//
LOCUS       DASWLN010000063         5430 bp    DNA     linear   ENV 20-FEB-2024
DEFINITION  MAG TPA_asm: Candidatus Zixiibacteriota bacterium isolate
            SMAG_U2949 k141_62305, whole genome shotgun sequence.
ACCESSION   DASWLN010000063 DASWLN010000000
VERSION     DASWLN010000063.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37960594
            Sequence Read Archive: SRR10376091
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Candidatus Zixiibacteriota bacterium (soil metagenome)
  ORGANISM  Candidatus Zixiibacteriota bacterium
            Bacteria.
REFERENCE   1  (bases 1 to 5430)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 5430)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (26-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/12/2024 08:28:26
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 1,440
            CDSs (total)                      :: 1,416
            Genes (coding)                    :: 1,412
            CDSs (with protein)               :: 1,412
            Genes (RNA)                       :: 24
            rRNAs                             :: 1 (16S)
            partial rRNAs                     :: 1 (16S)
            tRNAs                             :: 21
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 4
            CDSs (without protein)            :: 4
            Pseudo Genes (ambiguous residues) :: 0 of 4
            Pseudo Genes (frameshifted)       :: 0 of 4
            Pseudo Genes (incomplete)         :: 3 of 4
            Pseudo Genes (internal stop)      :: 1 of 4
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..5430
                     /organism="Candidatus Zixiibacteriota bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_62305"
                     /isolate="SMAG_U2949"
                     /isolation_source="soil"
                     /db_xref="taxon:2053527"
                     /environmental_sample
                     /country="USA: Oregon"
                     /lat_lon="42.99 N 123.63 W"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            complement(<1..999)
                     /locus_tag="VGB16_02180"
     CDS             complement(<1..999)
                     /locus_tag="VGB16_02180"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_020261925.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="aminotransferase class I/II-fold pyridoxal
                     phosphate-dependent enzyme"
                     /protein_id="HEX9916519.1"
                     /translation="MDIFEKCRNYTTAREVMAAGLYPYFTPLSSGQDTQVTINGKETI
                     MIGSNNYMGLTSHPKVKEAAMRAVELYGSGCSGSRFLNGTLDMHVELERRLAQFMKRE
                     AALVFSTGFQTNQGTISALVNKGEYMITDRSDHASIVDGCRLSFGKVLKFKHNNMKDL
                     ERVLRSIKKDAGKMIIVDGVFSMEGDIANLPKIVELAEKYNCQVMVDDAHSIGVLGEN
                     GRGTGEHFGLENKVDIVMGTFSKSFASLGGFIVADEEVIHYIKHTSRALIFSASMPPA
                     SVAATLAALDIIETEPQRRERLWQIAKKMHDGFKRLGFNIGETKTPIIPIYIGEDLQ"
     gene            1142..2125
                     /locus_tag="VGB16_02185"
     CDS             1142..2125
                     /locus_tag="VGB16_02185"
                     /inference="COORDINATES: protein motif:HMM:NF027681.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003824 - catalytic activity [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="NAD-dependent epimerase/dehydratase family
                     protein"
                     /protein_id="HEX9916520.1"
                     /translation="MSKALVTGANGFVGSHLVEYLLNKGYEVRCLVRKTSNLRWLSGL
                     NVEYHYGDARDKSSLVGAFKGCNYVFHVAGVTRGTSRKLYFDANLSGTQNVIETCLNE
                     NTSLKRFVYISSQAAVGPNPDQNPLDERAPCHPITPYGQSKLEAEKTVLQYKNKIPVT
                     IIRPPTVYGPRDDELFFVFKMIKCGIKPQIGFRDTLVSVCYIGDLIEGILLSAESEKS
                     IGQTYFIADDRVYSWKEATDMIAKSLERKAISIRFPKSILFLSAYFSEMFAKLSKKQA
                     LLNRYKAKELSQSYWICDISKAISDLGFVPKVKLEEGSKETAEWYKLNKWL"
     gene            2519..2917
                     /locus_tag="VGB16_02190"
     CDS             2519..2917
                     /locus_tag="VGB16_02190"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEX9916521.1"
                     /translation="MNWYLVIIALILSSILFVVLTLIAVLLGNWLKVHEDFGLKKILK
                     SLSTSYLFEFFIPFLVFVMLLFFSPFKGFRFGILFACLVFIFNSLEFFLFSSQGMTSL
                     RFDFTVYTLFWKLVKYLVSFGIAGLILGLI"
     gene            2931..5243
                     /locus_tag="VGB16_02195"
     CDS             2931..5243
                     /locus_tag="VGB16_02195"
                     /inference="COORDINATES: protein motif:HMM:NF027890.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="efflux RND transporter periplasmic adaptor
                     subunit"
                     /protein_id="HEX9916522.1"
                     /translation="MDWDYPKLRDDLIITLQIFQDVTYYVIKDPLTNKYFRVKEPEHF
                     ILRKLDGKTTFKEIVFDFEAKFGIPLKEEVFKGFVSRSANLGFFEMGETQREFTHLQY
                     QMSQKKNLLQRIIFIKLKAFDPDKFLEWLLKKFRFVFSRKFLFISLVLVFLAIVIILT
                     NWDGLKRSFADMINLGLVIKLWLAILLISGLHEIAHGLTCKYFGGEVREMGFLLLYFQ
                     PCFYCNVSDAWLFPDKKKKILVTFAGAYLNILLWATATIFWRVLDFDTWLGGFLFVVL
                     FLSSITTIFNFNPLIKLDGYYLLSDYLEIPNLRKKAFDFLANWFRKIILGFKEIKLTL
                     SGYEKKVYFSYGILALSYTILLVAIFFFRVENFLVSRYQGLGFVLFLILTFVIFKTPI
                     KVALAGIFSLFLSKRGIWMKPKRVIIYAAIIVVVLILLFLVKLELKISAECEIQPLES
                     YAISVSADGYVEEKIYKEGGQEKRSVNLSKLMSGDYALVNMESMVKEGDKVKPGDLIA
                     SFASSQNLSDLSSTQAKLESAIASYQLLKKGAQPEEISQAEDKVKQSEASLKLKQKDL
                     ERLRNLHKGKLISDEELERGEANFSIAQNGLEIERNQLKLMQRGARPEELKMARSEVD
                     QLESRVKFLEEQMASSQIKSPISGMVTSIQQQTNLISIANLDTVRVSIKVSEKDLDAI
                     YVGQKTKLKVRSYPWISFYGKVTKISNQAQEGSSKNIFLITSKVPNLELQLKPGMSGQ
                     AKIYCGKRSISNLLTRRIVRYLRVEVWSWW"
BASE COUNT         1575 a         1056 c         1181 g         1618 t
ORIGIN      
        1 ttgtaaatct tcaccaatat atattggaat aatcggggtt ttggtctcgc cgatattgaa
       61 acctaatctt ttgaaaccat cgtgcatctt cttggcgatc tgccacaacc tttctcgtct
      121 ttggggctca gtctcgatta tatctaaggc agccaaagtt gcggcaactg aagctggtgg
      181 catactggca gagaatatta aagcgcggga agtatgtttt atataatgaa ttacctcctc
      241 atccgctacg ataaatccac ctaaagaggc aaaagatttg gaaaatgttc ccatcacgat
      301 atcaacttta ttctccaagc caaaatgctc tccggtcccc cttccattct ctcctaatac
      361 tccaatggaa tgggcatcat ccaccatcac ctggcagtta tatttttctg ccagctctac
      421 aatcttaggt aagttggcaa tatctccttc catgctaaag accccatcca cgataatcat
      481 ctttccagca tccttcttaa tcgaacgcaa gaccctttct aagtccttca tattgttatg
      541 cttgaatttc aaaaccttac caaaggaaag ccgacatcca tccacaatgg aggcatggtc
      601 agagcggtca gttatcatat actcgccctt gttgaccaag gcggagatgg ttccctgatt
      661 ggtctgaaat ccggtagaga agactaaagc tgcctctctt ttcataaatt gggctaaacg
      721 tctttccagc tctacatgca tatccagagt tccgttcaaa aaccttgaac cggagcatcc
      781 ggagccataa agttccaccg ccctcattgc cgcctctttc accttgggat gactggtcaa
      841 gcccatatag ttgttcgagc cgatcattat cgtctctttg ccattgattg tcacctgggt
      901 atcctgacca gaggaaagag gagtaaaata gggataaagc ccggctgcca taacttctct
      961 ggcagtagtg taatttctac atttctcaaa aatatccata ttgaataatc gttttagagt
     1021 ttactttttg tccttgccaa ttgaagacaa aattgatatt ttagatttta agtcgatttt
     1081 ccattctaaa ccaaaaaagc acctttgtca agggaaaatt aaacccagag aggtatgatt
     1141 ggtgtctaag gctttggtta ctggcgcgaa tggatttgtg ggaagccatc tggttgaata
     1201 tctgttgaat aaggggtatg aggtgcgttg tctggtgaga aagactagta acttaagatg
     1261 gctttcggga ttgaatgtgg aataccatta tggggatgca agagacaaga gctctttggt
     1321 tggagctttt aaaggatgca attatgtttt tcacgtggct ggagttactc gaggcacgag
     1381 cagaaaacta tactttgatg ctaacttgag cggcacacag aacgtcattg aaacctgctt
     1441 aaatgagaat acaagcttga agaggtttgt ctatataagc agtcaggcag cagttggacc
     1501 caatccagac caaaatcctt tagatgaaag agcaccctgt cacccaatca ctccctatgg
     1561 gcaaagcaaa ttagaagcgg aaaaaactgt tctccaatat aaaaacaaaa taccagtaac
     1621 catcatccga ccaccaactg tatatggtcc cagagatgat gagctatttt ttgttttcaa
     1681 aatgataaag tgtgggatta agccccagat cggctttagg gacactctgg tcagcgtctg
     1741 ctatattggg gatttaatag aagggatttt actttccgcg gagagcgaga agtccattgg
     1801 gcagacatac ttcatagctg acgatagggt ctattcctgg aaagaggcaa ctgatatgat
     1861 agccaagtca ttggaaagga aagcgatttc tattcggttt cccaagagca tattgttcct
     1921 ctccgcctat ttttctgaaa tgtttgccaa gctctccaaa aaacaagcct tactcaatag
     1981 atacaaagcc aaagagctaa gccaaagtta ctggatatgt gatatttcta aggcaatatc
     2041 ggatttggga tttgtgccca aagtcaaatt ggaagaggga agcaaagaaa ctgctgaatg
     2101 gtataaactc aataagtggc tttagagaat acttagggtc tttcttaaat attggggagt
     2161 attttgtgta acctattgac aaaaggatag attttgtcat gctgaaccct gaaagtgtca
     2221 tgctgaactt ggttcagcat ctcttcaggg caggcttgtt tcagcatctc ttgagaccct
     2281 gaaatcgcag accctgaatt tgtttcaggg tatggttcag ggtgacaatc accaagggag
     2341 gggtgaagtc cgaagagtgc gggtggagat gcggttcgac cttgctcacc gtcctgagca
     2401 aagtcgaagg aaactccacc cctatcgcga gtacaacatg tattaccccg tggaggacaa
     2461 ctaaagaaaa tcgtagccaa acacaatttc cacattaaat aggaaagaac caatacttat
     2521 gaactggtat ctggtcataa ttgccttaat cctttccagt attttatttg tagtactcac
     2581 tttaattgcc gttcttttgg gcaattggct caaagttcac gaggattttg ggctgaagaa
     2641 gattttaaaa tctttatcca ccagctacct gtttgagttt ttcattccct ttcttgtttt
     2701 tgtgatgttg cttttctttt caccatttaa agggtttcgc tttgggatac tctttgcctg
     2761 cttggtcttt atttttaatt ccctcgagtt ttttcttttc tcttcacagg gaatgacaag
     2821 tttaagattt gattttacgg tttataccct tttctggaag ttggtcaaat atctggtcag
     2881 ctttggaatt gctggattga tactgggtct tatataatgg agctacctgt atggattggg
     2941 attatcccaa attaagagat gatttaatca tcactctaca aatatttcag gatgttacct
     3001 attatgtcat aaaggaccct ctcactaaca aatacttccg ggtaaaagag ccggaacatt
     3061 tcatcttgag gaaattggac ggaaagacca ctttcaaaga aatagttttt gacttcgaag
     3121 ccaaattcgg catcccctta aaagaagagg ttttcaaagg atttgtatcc cgctcagcga
     3181 atctgggatt ttttgaaatg ggggaaaccc aaagggaatt cacccatttg caataccaga
     3241 tgagccagaa gaaaaacctc ctgcaaagaa tcatctttat caaactcaaa gcctttgacc
     3301 cggacaagtt tttagagtgg cttttgaaaa aatttagatt tgtattttct cgaaagttct
     3361 tatttatctc gctggtttta gtctttttag caatagttat aattctgacc aattgggatg
     3421 gattaaagag aagcttcgcg gatatgatta atttaggtct ggtcattaaa ctctggcttg
     3481 ctattttgtt gataagcggc ttgcacgaaa tcgcccacgg cctcacctgt aagtattttg
     3541 gaggggaagt gcgtgagatg gggttcttgt tgctttattt tcaaccttgt ttttactgca
     3601 atgtgagcga tgcctggctt ttccctgata agaagaaaaa gatattagtt acctttgccg
     3661 gagcttattt gaatatactt ttatgggcaa ctgccacaat tttctggaga gtattggatt
     3721 ttgatacctg gctggggggc tttctgtttg tggtcctatt tctctccagc attactacca
     3781 ttttcaactt taaccccttg ataaagttag atggatacta tctattgagc gattacttgg
     3841 agattccgaa tctgcgcaaa aaggcatttg attttctagc caattggttc aggaaaatca
     3901 tattgggctt caaggagatt aagctaaccc tttctgggta tgagaagaaa gtttatttct
     3961 cttatggcat tttagcttta agttatacca ttcttttagt ggccatattc tttttcaggg
     4021 tggaaaactt cctggtctcc cgttatcagg gactgggatt tgtcttgttt ttgattctta
     4081 cgtttgttat ctttaaaaca ccaatcaaag tcgcacttgc cgggattttc agtttgtttt
     4141 taagcaaaag gggaatctgg atgaaaccga aaagggtaat aatctatgcc gctattatag
     4201 tagttgtact gattttgttg tttctggtta agttggagct aaagatttct gcggagtgcg
     4261 agattcaacc tttagagtct tatgctatca gtgtaagtgc ggacggatat gtagaggaaa
     4321 agatttacaa ggagggaggt caagaaaaaa gatcagttaa tctttctaag ctaatgtccg
     4381 gagattatgc tctggtcaat atggagtcta tggtaaagga aggggacaaa gtgaagcccg
     4441 gagacctaat cgcctccttt gcctccagtc agaacctctc tgacctttcc agcacacaag
     4501 ctaaattgga aagtgcgatt gccagttacc agcttttaaa gaagggtgct caacccgagg
     4561 agatatccca ggctgaagat aaggttaagc agagtgaagc cagcttaaag ttaaaacaaa
     4621 aagacctgga gaggctacgc aatctgcata aaggcaagct gataagcgat gaggaattgg
     4681 aaaggggaga ggcaaacttt tccatagctc aaaacggatt ggaaatcgaa cgtaaccaat
     4741 tgaaacttat gcagagagga gcaagaccag aggaactcaa gatggctcgc tcggaagttg
     4801 accagctgga aagcagggtt aagttcttgg aggaacagat ggcttcctct caaataaaaa
     4861 gccccatttc gggaatggtg acgagcattc aacagcaaac taacctcatc tctattgcca
     4921 atctggatac ggtcagggtc tcgattaaag tttcagaaaa ggatctagat gcaatttatg
     4981 ttggacagaa aacaaaactc aaggtgagaa gttatccctg gatttctttt tatggaaagg
     5041 ttactaaaat cagtaatcaa gctcaagagg gcagctcaaa aaacatattt ctgataacca
     5101 gcaaagttcc gaatttggaa cttcaactta agcccggaat gagtggccag gcaaaaatct
     5161 actgcgggaa aagaagcata tctaatttac tcacccgcag aattgtgcgt tatcttagag
     5221 ttgaagtttg gtcgtggtgg tgataaaagc agtttagaag tttactccgc accatacggc
     5281 gcagggttta ggagtttata agtttagaga tttgtgtagg ggttcgattg atcgaaccca
     5341 tttttttatc tttcgggttg gatgaatcac cacccctcat aataatgcac aataacgcag
     5401 cggatactac ggaatgccgc ctataaataa
//
LOCUS       DASWLN010000064         2952 bp    DNA     linear   ENV 20-FEB-2024
DEFINITION  MAG TPA_asm: Candidatus Zixiibacteriota bacterium isolate
            SMAG_U2949 k141_315707, whole genome shotgun sequence.
ACCESSION   DASWLN010000064 DASWLN010000000
VERSION     DASWLN010000064.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37960594
            Sequence Read Archive: SRR10376091
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Candidatus Zixiibacteriota bacterium (soil metagenome)
  ORGANISM  Candidatus Zixiibacteriota bacterium
            Bacteria.
REFERENCE   1  (bases 1 to 2952)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 2952)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (26-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/12/2024 08:28:26
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 1,440
            CDSs (total)                      :: 1,416
            Genes (coding)                    :: 1,412
            CDSs (with protein)               :: 1,412
            Genes (RNA)                       :: 24
            rRNAs                             :: 1 (16S)
            partial rRNAs                     :: 1 (16S)
            tRNAs                             :: 21
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 4
            CDSs (without protein)            :: 4
            Pseudo Genes (ambiguous residues) :: 0 of 4
            Pseudo Genes (frameshifted)       :: 0 of 4
            Pseudo Genes (incomplete)         :: 3 of 4
            Pseudo Genes (internal stop)      :: 1 of 4
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2952
                     /organism="Candidatus Zixiibacteriota bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_315707"
                     /isolate="SMAG_U2949"
                     /isolation_source="soil"
                     /db_xref="taxon:2053527"
                     /environmental_sample
                     /country="USA: Oregon"
                     /lat_lon="42.99 N 123.63 W"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            <1..514
                     /locus_tag="VGB16_02200"
     CDS             <1..514
                     /locus_tag="VGB16_02200"
                     /inference="COORDINATES: protein motif:HMM:NF024634.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=2
                     /transl_table=11
                     /product="4Fe-4S binding protein"
                     /protein_id="HEX9916523.1"
                     /translation="AVVCMDGDGPSSGKPKYLGLILASADAVALDKVCSRIFGFKEDE
                     IETTNIAEERGLGTADFSKIELVGEKLSDIALPKFDLPSNRFLKMVPKFMVKLLEPYV
                     WVRPNVKEELCTNCNLCVENCPMKVIQKDSKRPNFNYAECINCLCCHELCPENAVYLE
                     KSWLARKFIH"
     gene            complement(826..2721)
                     /locus_tag="VGB16_02205"
     CDS             complement(826..2721)
                     /locus_tag="VGB16_02205"
                     /inference="COORDINATES: protein motif:HMM:NF022481.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF2723 domain-containing protein"
                     /protein_id="HEX9916524.1"
                     /translation="MPSKLYLSLCGGLIFLFSGIVYLLTLSPTLSFIDSGELAAVCAT
                     LGIAHPPGYPLYAQLGRISSFLPFGEPIYRFNLMSAFWVSLTNLCLFLSLILISYIFF
                     SKELKTVLKLSAALLITILFSFSRILWSQALINEVYSLNIFLCSLIILIALYWYKTNL
                     NPSKSDTAISPLVYLLTYLIGLSSGNHFLSLLLLPALLYLLISSTKLSSYSSKSLFLI
                     VGVLIIGVSIYLYLPIRAAQNPALNWGNPSNWLNLKNHVSARMFQGRMFSETELVFWD
                     NLKHLSSVFFKQFPVWAFPLMLLGILKAFKKNSKIAIFFLLVILFNLLWSLNYGVKDI
                     DPYFLQTILISSFLLYLGFLFFFQIIEKVLSELKSSGKLKFVSSYMIIFILGIFFLSR
                     FPQEFRAQNRSKNYVPYDFALNILRSSHKDALILTEVWDYYSAWLYLRFVENKRPDLE
                     IIQTELLQWSWYKDYIRKYYPATYKRSFEQIEIYNQFALSMEKGQPVNSLDASMALSK
                     MIDSFIFKNLKDRPVYTTLLDDYKRYAGLPTITEGLLMRFREEKKFYPYEFPEFRLGG
                     ILDPAILKTNKEIVCISFYAVSYYNRGLYLKFFDQNEEANRYFQKAYYFKEVFLKHNP
                     GFDFEYF"
BASE COUNT          978 a          520 c          655 g          799 t
ORIGIN      
        1 tgcggttgtc tgtatggatg gagatggccc ctcttccggc aagccgaaat acctgggact
       61 gattctggca agtgcagatg cggttgcttt agataaagtc tgctccagaa tttttggatt
      121 taaagaggat gaaatcgaaa ctaccaatat cgcagaggaa agaggtttgg gcacagctga
      181 tttttcgaag atagagctgg ttggcgagaa attgtccgat atcgctctcc caaaattcga
      241 tttgccctcg aatagattct tgaaaatggt acccaagttt atggttaagc tcttggagcc
      301 gtatgtttgg gtgagaccta atgtcaaaga ggagctttgc accaattgca acctctgcgt
      361 ggagaactgc ccgatgaagg tgattcaaaa agattccaaa agaccaaact tcaactatgc
      421 agaatgtatc aactgcttat gctgtcatga gctttgccct gaaaatgcag tttatctgga
      481 gaaaagctgg ctggcgagga agttcataca ttaaaaagat tttatcaagc aactattcag
      541 acaatatgtc attccgcact tgatgttgaa aacccagtag ttagggcgga atccattttt
      601 aaagttgttt tctctctttt tggatttctc gtatcgagta cgggacaggc tctgctggag
      661 tttatcccgc agtaatgcgg gacgggaatg acaaggatac aggttctcgt tggagttgaa
      721 catctaccag gtgaaggtcg tcataaggct ttaagataga ttacacccga aagaaataaa
      781 gataactaca tggaggggtt acttttttaa tatttttggg catagttaga aatattcaaa
      841 atcaaaaccg ggattatgct tgaggaagac ctccttgaaa tagtaagcct tttgaaaata
      901 tctatttgct tcttcattct gatcaaagaa tttgaggtat aaacctcgat tgtaatatga
      961 aacagcataa aaagaaatgc aaactatctc cttgttggtc ttaagaattg caggatccaa
     1021 gataccaccc aatctaaact cagggaactc gtaaggatag aatttctttt cttctctaaa
     1081 acgcatcaat aatccttcag ttatagttgg aaggccggca tatcttttgt agtcatctaa
     1141 gagggtagta tatactggtc gatccttgag attcttgaag atgaaactat caatcatctt
     1201 tgataaagcc atagatgcat ctaaggaatt cacaggctga cccttttcca tacttagagc
     1261 aaactgatta taaatctcaa tctgctcaaa agagcgttta taagtagccg ggtagtattt
     1321 ccgaatataa tccttgtacc agctccattg tagaagttcg gtttggatga tctccagatc
     1381 aggtcttttg ttttcgacga atctgagata aagccaggca gaataataat cccagacctc
     1441 tgttagaatt agggcatctt tatgcgagga cctcagaata tttaaggcga aatcataagg
     1501 aacgtaattt ttgcttcggt tttgggctct aaattcttgt ggaaatcttg aaaggaaaaa
     1561 aatccccagt ataaatataa tcatatagct tgaaacaaat ttcaatttac cagatgattt
     1621 aagctctgat aagaccttct ctatgatttg gaagaaaaag agaaacccta agtaaagtag
     1681 gaaagatgag atcaaaatcg tctgcagaaa ataagggtcg atatctttta ctccgtagtt
     1741 tagactccaa aggagattga aaagaattac tagcagaaaa aagatggcga ttttcgagtt
     1801 ctttttgaag gctttcagaa tacccaagag cataagcgga aaagcccaaa ccggaaattg
     1861 tttaaagaaa actgagctaa gatgcttgag gttgtcccag aacacaagtt cggtttcgct
     1921 gaacatacga ccttgaaaca tcctggcact gacatggttc tttagattca accagttgga
     1981 aggattaccc cagttaagag cagggttttg tgctgctcgg ataggaagat agagatatat
     2041 tgaaactcct atgattagaa ccccgacaat taagaataaa gattttgagc tgtagctact
     2101 aagtttagtc gaggagatca ataggtaaag aagtgcaggc agtaaaagta gacttagaaa
     2161 atggttacca gagcttaatc caataagata ggttaaaaga taaaccagag gggagatggc
     2221 agtatctgat ttgctaggat taagatttgt cttataccag tataaagcaa ttagaatgat
     2281 caaactacaa agaaagatat ttagggaata gacttcattt ataagagctt gcgaccataa
     2341 gatccgggaa aaggagaaga ggatggtaat caaaagtgcg gctgaaagtt tgagtacggt
     2401 tttgagctct tttgagaaaa aaatgtacga gattaggatt agagatagaa acaagcaaag
     2461 attagttaag ctaacccaga aagcagacat taggttgaat ctgtagatgg gttctccaaa
     2521 aggaagaaag gaagatattc ttcccagttg tgcatacaat ggataaccag gaggatgagc
     2581 gatccccaaa gtggcgcaga cagcggccaa ttcaccggaa tcaataaaag aaagagtagg
     2641 agaaagcgtt aagagataaa caataccact aaagagaaaa atcaaaccac cacacaggga
     2701 aagataaagc tttgagggca taatttttga tcttaaacca gaaattttgt tttgcctgaa
     2761 ggtttaaggt tagttaccca ttcgacagaa agctatgggc ataaacctgt ggatgaatag
     2821 cactctgttc aaggctccgc tccatggaac cctacacgac aagatgccgc agccgtcctt
     2881 cgacataatc aggccttcgg ctctacactt cgtcctgccc ttcggtcccg agatctcagg
     2941 gtcgagggaa ac
//
LOCUS       DASWLN010000065         7065 bp    DNA     linear   ENV 20-FEB-2024
DEFINITION  MAG TPA_asm: Candidatus Zixiibacteriota bacterium isolate
            SMAG_U2949 k141_572010, whole genome shotgun sequence.
ACCESSION   DASWLN010000065 DASWLN010000000
VERSION     DASWLN010000065.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37960594
            Sequence Read Archive: SRR10376091
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Candidatus Zixiibacteriota bacterium (soil metagenome)
  ORGANISM  Candidatus Zixiibacteriota bacterium
            Bacteria.
REFERENCE   1  (bases 1 to 7065)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 7065)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (26-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/12/2024 08:28:26
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 1,440
            CDSs (total)                      :: 1,416
            Genes (coding)                    :: 1,412
            CDSs (with protein)               :: 1,412
            Genes (RNA)                       :: 24
            rRNAs                             :: 1 (16S)
            partial rRNAs                     :: 1 (16S)
            tRNAs                             :: 21
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 4
            CDSs (without protein)            :: 4
            Pseudo Genes (ambiguous residues) :: 0 of 4
            Pseudo Genes (frameshifted)       :: 0 of 4
            Pseudo Genes (incomplete)         :: 3 of 4
            Pseudo Genes (internal stop)      :: 1 of 4
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..7065
                     /organism="Candidatus Zixiibacteriota bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_572010"
                     /isolate="SMAG_U2949"
                     /isolation_source="soil"
                     /db_xref="taxon:2053527"
                     /environmental_sample
                     /country="USA: Oregon"
                     /lat_lon="42.99 N 123.63 W"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            331..525
                     /locus_tag="VGB16_02210"
     CDS             331..525
                     /locus_tag="VGB16_02210"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEX9916525.1"
                     /translation="MHSKKDYQRNKNSHYCTASKGEGEEYDGCEAIGVFPFPLPGRAG
                     TLSLSLKGREKPEEITIKTF"
     gene            complement(562..1629)
                     /gene="ispG"
                     /locus_tag="VGB16_02215"
                     /gene_synonym="gcpE"
     CDS             complement(562..1629)
                     /gene="ispG"
                     /locus_tag="VGB16_02215"
                     /gene_synonym="gcpE"
                     /EC_number="1.17.7.1"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_007953566.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0046429 - 4-hydroxy-3-methylbut-2-en-1-yl
                     diphosphate synthase activity [Evidence IEA];
                     GO_process: GO:0044237 - cellular metabolic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="flavodoxin-dependent
                     (E)-4-hydroxy-3-methylbut-2-enyl-diphosphate synthase"
                     /protein_id="HEX9916526.1"
                     /translation="MEMIKRRKTKKIQVGKLDIGGDAPISVQSMTKTDTRNVEETIRQ
                     IRNLERVGCELVRVALPDKESALAISKIKKRISIPLIADIHFDYRLALLAIDSGADKL
                     RLNPGNIRTKWKVKEVVKAASQRGIPIRVGANSGSLPKSLLSKYKGPTPQAMIEAVMQ
                     QIRLLEDMSFSNIVVSLKATDVSTTIESYRRISSKIDYPLHLGVTEAGPPNTGIVRSS
                     IGMGVLLCMGIGDTIRVSLTADPVEEVKTGYRILQSLGLREYGPILISCPTCGRCELN
                     LLQITKQVEKELSKIKSPIKVAVMGCVVNGPGEAREADVGIAGAKGYGLLFRKGKIAR
                     KVKEKDLVRALLQEIENIVKR"
     gene            1690..2043
                     /locus_tag="VGB16_02220"
     CDS             1690..2043
                     /locus_tag="VGB16_02220"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEX9916527.1"
                     /translation="MPISSKLRLPLIISAILIVVLVIWIVHRKTSSRLTPERFAQVYV
                     ELSIAYEACGSDVAKWHIEKARILKEHNLSTEEIKRFITKYNQNPSEWVLLWERIVNR
                     LHQEQQHLANPAPLP"
     gene            complement(2021..2398)
                     /locus_tag="VGB16_02225"
     CDS             complement(2021..2398)
                     /locus_tag="VGB16_02225"
                     /inference="COORDINATES: protein motif:HMM:NF009154.0"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003676 - nucleic acid binding [Evidence
                     IEA];
                     GO_function: GO:0004518 - nuclease activity [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="YraN family protein"
                     /protein_id="HEX9916528.1"
                     /translation="MLENRRQQLGKEGEDIAADFLKNIGFVILERNFKYGRKDIDIIC
                     RDGKTIVFVEVKAGRSESFGTPLEKVHQRKQKNIAQVAKSFMQQKDCAGHDFRFDVVA
                     IDDKKVSKKIKHLRNAFMVEELD"
     gene            complement(2410..3048)
                     /locus_tag="VGB16_02230"
     CDS             complement(2410..3048)
                     /locus_tag="VGB16_02230"
                     /EC_number="3.1.26.4"
                     /inference="COORDINATES: protein motif:HMM:NF000594.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003723 - RNA binding [Evidence IEA];
                     GO_function: GO:0004523 - RNA-DNA hybrid ribonuclease
                     activity [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="ribonuclease HII"
                     /protein_id="HEX9916529.1"
                     /translation="MPIHLGHERKLWKEGIKLIAGVDEVGRGPLAGPVVAAAVILPPR
                     INLKGIKDSKKLSFQKREEFFQKISDLALSVGVGIVDEKRIDGVNILNASLEAMRSAI
                     LNLNVRPEFVLVDGNKEIPGLDIPQLAIIAGDESSLFVACASVIAKVTRDRLMLKYHQ
                     TYPQFHFDQNKGYGTKLHLDALKTYGPCEIHRFSFNPVKLCKERQRPLKFKN"
     gene            complement(3054..3434)
                     /gene="rplS"
                     /locus_tag="VGB16_02235"
     CDS             complement(3054..3434)
                     /gene="rplS"
                     /locus_tag="VGB16_02235"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_013430653.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0000311 - plastid large ribosomal subunit
                     [Evidence IEA];
                     GO_component: GO:0022625 - cytosolic large ribosomal
                     subunit [Evidence IEA];
                     GO_function: GO:0003735 - structural constituent of
                     ribosome [Evidence IEA];
                     GO_process: GO:0006412 - translation [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="50S ribosomal protein L19"
                     /protein_id="HEX9916530.1"
                     /translation="MDLMAGLEGKYLKTKTKDFKPGDTIRVQVKVKEGDRERIQTFEG
                     VVIQKKGKGTGETFTVRKVSAGVGVERVFPLHSPNLSRIEVVRSGKVKRAKLFYLRKM
                     SGKSAKIDEERKEKQEKETNEAST"
     gene            complement(3448..4182)
                     /gene="trmD"
                     /locus_tag="VGB16_02240"
     CDS             complement(3448..4182)
                     /gene="trmD"
                     /locus_tag="VGB16_02240"
                     /EC_number="2.1.1.228"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_019424957.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0052906 - tRNA
                     (guanine(37)-N1)-methyltransferase activity [Evidence
                     IEA];
                     GO_process: GO:0008033 - tRNA processing [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="tRNA (guanosine(37)-N1)-methyltransferase TrmD"
                     /protein_id="HEX9916531.1"
                     /translation="MKIDILTIFPEMVESPLKQSLIGRALEKGIVEIKIKDIRDFAKD
                     KHATVDDVPFGGGSGMVMKIEPLTLALESVLDGSVQSRIVLTTAQGRRFDQKKAKELS
                     LEKHLVIICGHYKGVDERIKELFPLEEMSIGDYVLTGGEFASLVIVDAVVRLIPGVLG
                     NFESAQTDSFLEGILGYSEYTRPKEFRGKKVPEVLVSGDHKKIRLWRREKALRKTLET
                     RPELLQDEMLSSEDLAFINKLRSGKI"
     gene            complement(4189..4701)
                     /gene="rimM"
                     /locus_tag="VGB16_02245"
     CDS             complement(4189..4701)
                     /gene="rimM"
                     /locus_tag="VGB16_02245"
                     /inference="COORDINATES: protein motif:HMM:TIGR02273.1"
                     /note="Essential for efficient processing of 16S rRNA;
                     Derived by automated computational analysis using gene
                     prediction method: Protein Homology.
                     GO_function: GO:0003723 - RNA binding [Evidence IEA];
                     GO_process: GO:0006364 - rRNA processing [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="ribosome maturation factor RimM"
                     /protein_id="HEX9916532.1"
                     /translation="MPSCDKVAIGLISRVRGIKGEVVVAPLTDDSKRFSKIKKIQLFL
                     PEGYRTFSIQSSRRFKDVVIIKLAGIDNPEDGKKLVGCYLEIEKDEVAPLPKGRYYIF
                     DIVGLKVRTPEGEDLGEVKEVIQYPANDVYVISHQGKEFDLPATKEIIKDIDLEKRVM
                     VVQPLAGIFD"
     gene            complement(4726..5157)
                     /gene="rpsP"
                     /locus_tag="VGB16_02250"
     CDS             complement(4726..5157)
                     /gene="rpsP"
                     /locus_tag="VGB16_02250"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_014559643.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0000314 - organellar small ribosomal
                     subunit [Evidence IEA];
                     GO_component: GO:0022627 - cytosolic small ribosomal
                     subunit [Evidence IEA];
                     GO_function: GO:0003735 - structural constituent of
                     ribosome [Evidence IEA];
                     GO_process: GO:0006412 - translation [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="30S ribosomal protein S16"
                     /protein_id="HEX9916533.1"
                     /translation="MAVKIRLRRMGAKKKAYYRFVIADSRSPRDGRFIEAIGTYNPLE
                     KPAKVILQEDRVYHWLDKGALPSATANALFRQVGLLKKWSMMRKKQDVSAIQLKTTIR
                     ERGKKKRKRKTDGAAKAEKAPKPVKAEEKKEEPKQEDKGKE"
     gene            complement(5176..6498)
                     /gene="ffh"
                     /locus_tag="VGB16_02255"
     CDS             complement(5176..6498)
                     /gene="ffh"
                     /locus_tag="VGB16_02255"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003516319.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0048501 - signal recognition particle,
                     plasma membrane targeting [Evidence IEA];
                     GO_function: GO:0003924 - GTPase activity [Evidence IEA];
                     GO_function: GO:0005048 - signal sequence binding
                     [Evidence IEA];
                     GO_process: GO:0009306 - protein secretion [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="signal recognition particle protein"
                     /protein_id="HEX9916534.1"
                     /translation="MFEELAIKFENITRKLRGYGKLTEDNIRDSLREVRKALLEADVN
                     YKVVKKFIDDIQEKSLGEKTTRSIRPGEQIIKIVYDQLCELLGGKVEELKYSLKPPTV
                     FMLVGLQGSGKTTACAKLAKYIKKKGRNPLMVAADTYRPAAAKQLEVLGKTLNIEVFS
                     SGKNPIEISKKAVNKARQEGFDLVLLDTAGRLHIDEDLMQELCQIKKEVSPSETVLVV
                     DAMTGQEALNIAQTFDQRVGLDSIFLTKLDGDARGGAALSIRAMAGKPIKFVGIGEKL
                     DQMELFHPERMASRILGMGDVVSLVEKAQEVISVEEARKLEEKLKKESYTLEDFYEQL
                     QQIKKMGPLESILKMIPGLGGKALQNVQVDDKSMNRVEAMINSMTPEERKNPYILDGS
                     RKKRVAKGSGTSVQEINRLVKQFFTMQKMIKDLGKMDFKNFKGMASFT"
     gene            complement(6504..>7065)
                     /gene="rpe"
                     /locus_tag="VGB16_02260"
     CDS             complement(6504..>7065)
                     /gene="rpe"
                     /locus_tag="VGB16_02260"
                     /EC_number="5.1.3.1"
                     /inference="COORDINATES: protein motif:HMM:TIGR01163.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004750 - D-ribulose-phosphate 3-epimerase
                     activity [Evidence IEA];
                     GO_process: GO:0006098 - pentose-phosphate shunt [Evidence
                     IEA]"
                     /codon_start=2
                     /transl_table=11
                     /product="ribulose-phosphate 3-epimerase"
                     /protein_id="HEX9916535.1"
                     /translation="LHLDIMDGHFVPNITFGPMIVSAIDQCTNLILDVHLMIDKPEDY
                     LERFVKSGADWITFHWESIKNPEELISRMRELKVKVGCSLRPQTSFESVKGIMKELDL
                     LLLMTVNPGFGGQGFMMEVLPKIREARRFIRENKLHTVIQVDGGINPETSKLTQEAGA
                     DILVAGEAIFKKKDYREAIAQIRQGK"
BASE COUNT         1753 a         1796 c         1245 g         2271 t
ORIGIN      
        1 ccagggactt ctcttgtaaa attcccacat taaatacgaa agagcttaaa ttaatgcgtt
       61 tgtattaaaa taactaccag cactatcccc taaaacagga catggaaaga gggaaccgtt
      121 tgtccgaatt ctggatcttg agacccgaag tctgggtctt gagatgagat gaggagaatt
      181 gaagcattgg aaagcgggaa aacaattcct gtctttaaga aaggaatgct cttctttgag
      241 ggtgtctaag aactagagtt ttggctagac cgtagagaac cttatggagt gcatcccgcc
      301 atcggcggga tgcatgcatt gacacggtca atgcactcca aaaaagatta ccaaagaaat
      361 aaaaattcgc attattgcac agcctcaaaa ggtgagggtg aggagtatga tggctgtgag
      421 gctattgggg tatttccttt ccccctccct ggccgggcag gcactctatc tctctccctt
      481 aaagggagag agaaaccgga ggagataacc attaagacat tttgatgtat attgaagaaa
      541 agtggggata ctcctcaata actatctctt gactatattt tctatttcct gcaatagggc
      601 tctaactaaa tctttttctt ttacttttct cgcgattttc cccttcctaa aaagtaatcc
      661 atagcctttt gcacctgcaa ttcccacatc cgcttccctg gcttcacctg ggccattgac
      721 cacgcatccc ataaccgcaa ccttaatggg cgatttaatc ttgctcaatt ctttctcaac
      781 ctgctttgtt atttgtaaaa ggtttagctc gcatcttcca caagtcggac aggaaattaa
      841 gattggcccg tattctctta aacctagaga ttgcagtatc cggtatcctg tcttcacttc
      901 ttcgaccggg tcggcagtca aggaaacacg aatggtgtcg ccaattccca tacagagcaa
      961 tacgcccata ccgatgctcg accttactat gcctgtatta ggaggaccag cctcggttac
     1021 gcccaaatgc agtggataat ctatcttgct cgatattcgt ctgtatgatt caattgtggt
     1081 tgaaacatct gtcgctttga gcgatactac tatgttggag aaactcatat cttcaagaag
     1141 gcgaatttgc tgcataaccg cttctatcat tgcctgtgga gtcggtcctt tgtatttgga
     1201 caataaactt ttgggaagcg aaccggaatt agccccaact cttatcggaa tacctctttg
     1261 agaagcagct tttactacct cttttacttt ccacttggtt ctaatgttgc ccggatttaa
     1321 acgcaacttg tccgcgccag agtcaatagc caatagtgcc agtctgtagt caaaatgaat
     1381 gtcagctatc aaaggaatag atattcgttt tttaatcttg ctgatagcta aggcggactc
     1441 tttgtccggc agcgccacac gaacaagctc gcatcccacc ctctccaagt ttcttatctg
     1501 cctgatagtc tcttctacgt tgcgcgtatc tgttttggtc atcgattgaa ctgagatggg
     1561 cgcatctccg cctatgtcta actttcctac ttgaattttt ttagtctttc ttcttttaat
     1621 catctctata gaatgggctt gacagtcgct tttttaatat taatttgaac tcttacaggt
     1681 ttcaaaacta tgccgatttc atccaaactt agacttccct taattatatc tgctattcta
     1741 atagtagttt tggttatctg gatagttcac cgaaaaacat cctctagatt aactccggaa
     1801 cgtttcgccc aggtctatgt tgaattgtcc atcgcctatg aagcttgcgg ctcggatgtt
     1861 gctaaatggc acatagagaa agcaaggatt ttgaaagagc ataacctttc cacagaagaa
     1921 ataaaacgat tcataacaaa atataaccaa aatccttcgg aatgggttct cctgtgggaa
     1981 agaattgtaa atcgtctgca ccaggagcag caacacttag ctaatccagc tcctctacca
     2041 taaaggcgtt acgtagatgt tttatctttt ttgatacttt cttgtcgtca atcgccacaa
     2101 catcaaatct gaaatcatgc ccggcacaat ccttctgttg cataaaagat ttagctacct
     2161 gagcaatatt cttctgcttt ctttgatgaa ccttttccaa aggagtgccg aaactttctg
     2221 aacgtccggc tttcacttca acgaagacga tggtcttacc atctctgcag attatatcta
     2281 tatccttacg gccatatttg aaattacgtt ccaagattac aaatccgata tttttcaaga
     2341 aatcggctgc aatatcctcg ccttctttgc ccaattgttg tcttcgattt tctagcattt
     2401 caaaaacctt cagtttttaa atttcagggg tctttgcctc tctttgcaga gcttaaccgg
     2461 attgaagctg aaacgatgga tttcacacgg tccgtaggtt tttagagcat ctaaatgcag
     2521 tttggtgccg tatcccttgt tttggtcaaa atgaaactgt ggataggttt ggtggtattt
     2581 cagcatcagg cggtcgcgcg taaccttagc tatgaccgaa gcacaagcca caaataaact
     2641 ggactcgtcc ccagcaatta tggcaagctg tggaatatcc agtccgggga tttccttatt
     2701 cccgtccact aagacaaact cgggccttac gttcaaattc aagatggctg aacgcatcgc
     2761 ttccagagag gcatttagga tattcactcc atctatcctt ttttcatcca ctatgcctac
     2821 gcccacactc aaagccaaat cggaaatctt ttgaaaaaac tcttccctct tttgaaaaga
     2881 cagcttcttg gaatctttta ttccctttaa gttaattctt gggggtaaaa ttactgcggc
     2941 tgcaactacc ggacccgcca agggacccct acccacctca tccacaccgg caatcagctt
     3001 tattccttcc ttccacaatt tccgctcgtg ccctaagtga atcggcatct tttctaagtg
     3061 ctagcttcgt tggtttcctt ttcttgtttt tcctttctct cctcgtctat tttggcagac
     3121 ttaccagaca tcttgcgcaa ataaaaaagt tttgccctct taacctttcc agacctaacc
     3181 acttctatcc tggataagtt aggagagtgc aaaggaaaca ccctttccac tccaactccg
     3241 gccgagacct ttcgcaccgt gaaggtttct ccagttccct tccctttttt ctgtatcacc
     3301 actccttcga aggtttgaat tctttccctg tctccctcct tcactttcac ctgtactctg
     3361 attgtatctc cgggtttaaa atcttttgtc tttgttttca gatactttcc ttcaaggcct
     3421 gccatcaaat ccatatttcc tccagcttta gattttaccg cttctcagtt tatttatgaa
     3481 cgccaaatcc tctgagctaa gcatttcgtc ctgcagcaac tctggtcttg tctccagtgt
     3541 ctttcttaag gctttctccc ttcgccataa ccttattttt ttatggtctc cggagactaa
     3601 gacctctgga accttttttc ctctgaattc ttttgggcga gtatattccg aataacccaa
     3661 aatgccttca aggaacgaat cagtctgagc tgattcaaaa ttgcctaaaa ctccgggaat
     3721 cagcctcacg actgcgtcca ctatgactaa gctggcgaat tcgccaccgg ttaaaacata
     3781 atccccaatc gacatctcct caagcggaaa aagctctttt attctctcat ctaccccctt
     3841 gtaatgaccg cagattatga ccaaatgttt ctccaaagaa agctcttttg ctttcttctg
     3901 gtcaaatctt cttccctggg cggtggtcag gactatcctg ctttgaacgg aaccatctaa
     3961 gaccgattcc aaagctaaag tcaaaggctc tatcttcatc accatacccg acccaccacc
     4021 aaagggaaca tcatccaccg tagcatgctt atctttggcg aagtcccgga tgtctttaat
     4081 tttgatttca actatccctt tttccaaagc cctaccaatg agactctgtt tcagagggga
     4141 ttcgaccatc tcggggaaaa tggttaagat gtcaattttc attttgggtt aatcgaaaat
     4201 tccagctaag ggttgaacaa ccatcactct tttctctaaa tctatgtctt ttattatctc
     4261 cttggtggct ggcaggtcaa attctttccc ctggtgagat ataacgtaaa catcattggc
     4321 cggatattga attacctctt taacctctcc caaatcctcc ccttcaggtg ttctcacctt
     4381 gagaccaact atatcgaaaa tgtaatatct ccctttgggc aaaggagcaa cttcatcctt
     4441 ttctatttcc aaatagcatc ctactaattt tttaccatcc tctggattgt cgattccagc
     4501 cagtttaatg attactacat ccttgaatct tctgctgctc tggatactga aggtgcgata
     4561 tccttccggt aaaaagagtt gtattttctt tatctttgag aatctcttcg agtcatcagt
     4621 caaaggagca actactactt ctccttttat ccctcgaacc cgagaaatca aaccaatagc
     4681 cactttatcg caggagggca ctcatcctcc tttgtccaga agtccttatt cttttccctt
     4741 atcttcctgc tttggttcct cttttttttc ttcagctttt actggtttgg gcgccttttc
     4801 cgctttggct gcaccatccg ttttcctttt cctcttcttc tttccccgtt cccgaatagt
     4861 cgttttcagc tgaatggcac taacatcctg cttctttctc atcatgctcc acttcttcaa
     4921 taaccccacc tgacggaaaa gagcattcgc agttgcactg ggcaacgctc ctttatccag
     4981 ccaatggtaa accctgtctt cctgaagaat gaccttggct ggtttttcga gcggattata
     5041 agttccaatt gcttcgatga atctcccgtc tctgggacta cgtgaatcag ctatcacaaa
     5101 acgatagtat gccttcttct tggcacccat acgacgcaac cttatcttga ctgccaaatc
     5161 tacctccttt attgtttaag tgaaggaagc cattccctta aagttcttga aatccatctt
     5221 ccctaaatcc ttaatcattt tctgcatagt gaagaactgc tttactaagc gatttatctc
     5281 ttgaaccgaa gttccactcc ccttagcgac tctttttttc ctgctgccat ccaggatata
     5341 aggatttttt ctctcctccg gagtcataga gttaatcatc gcttccacac gattcatgga
     5401 cttatcgtcc acctgcacat tttgcagggc ctttcctcca agcccgggaa tcatttttaa
     5461 aatagattcc aaaggtccca tctttttaat ctgctgcaac tgctcataga agtcttcgag
     5521 agtgtagctt tcctttttca gtttctcttc cagctttctt gcttcctcca cgctgatgac
     5581 ttcttgtgct ttttccacca aggaaaccac gtctcccata cccaggattc tggaagccat
     5641 gcgctctgga tgaaaaagtt ccatctggtc aagtttttca ccgattccta caaacttgat
     5701 tggcttgccc gccattgccc tgatagacaa ggctgctcct ccgcgggcat caccatccag
     5761 cttagtcaag aatatgctgt ctaaaccgac tctctgatca aaggtttgag caatattgag
     5821 tgcctcttgt ccagtcatag catctactac caaaacggtc tcgctcggag aaacttcctt
     5881 tttaatctgg caaagctcct gcattaagtc ctcatcgata tgcaatcttc cagcagtatc
     5941 caatagtact aggtcgaaac cttcttgtct tgccttatta accgcctttt tgcttatttc
     6001 gattggattt ttccctgaag agaagacctc aatattcaat gtctttccta aaacctccag
     6061 ctgtttggca gctgctggtc ggtatgtgtc cgctgccacc attagaggat ttctcccctt
     6121 tttcttgatg tatttagcca gtttggcaca agccgtggtc ttgcctgaac cctgcaaacc
     6181 gactaacatg aaaacggtgg gtggctttaa agagtactta agttcttcta ctttcccacc
     6241 caatagctcg cacaactgat catagactat ctttattatc tgttccccgg gccttatgct
     6301 tcttgttgtc ttctcaccca gagatttctc ttgaatgtca tcaatgaatt ttttaactac
     6361 tttataattg acatccgctt ccagaagtgc ctttcttact tccctcaagg aatctctgat
     6421 attatcctcg gtcagcttcc catatccccg aagcttcctt gtaatgtttt caaattttat
     6481 ggctaattct tcgaacattt tttctatttt ccttgcctga tttgtgctat ggcttctctg
     6541 taatctttct ttttgaatat cgcctcgccc gccacaagta tgtccgcacc tgcctcttga
     6601 gtcaatttgg aggtttccgg atttatccct ccgtcaactt gaatgactgt atgtaactta
     6661 ttttcacgga tgaatcttct ggcttctctt atcttaggca ggacctccat cataaacccc
     6721 tgtccgccaa acccgggatt aaccgtcatt aaaagcaaga ggtctaattc cttcatgatt
     6781 cccttaactg actcaaaaga cgtttgtggc cttagggagc aacctacttt gactttcaat
     6841 tccctcattc tggatatcag ctcttctggg ttcttgatac tttcccagtg aaaggttatc
     6901 cagtcagcgc cacttttaac aaaacgctcc aagtaatctt ctggcttatc tatcatcaaa
     6961 tgcacatcca ggattagatt ggtgcattgg tcaatagcag acactatcat tgggccgaaa
     7021 gttatattgg gcacaaaatg tccatccatt atatccaaat gcaaa
//
LOCUS       DASWLN010000066         2161 bp    DNA     linear   ENV 20-FEB-2024
DEFINITION  MAG TPA_asm: Candidatus Zixiibacteriota bacterium isolate
            SMAG_U2949 k141_1045059, whole genome shotgun sequence.
ACCESSION   DASWLN010000066 DASWLN010000000
VERSION     DASWLN010000066.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37960594
            Sequence Read Archive: SRR10376091
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Candidatus Zixiibacteriota bacterium (soil metagenome)
  ORGANISM  Candidatus Zixiibacteriota bacterium
            Bacteria.
REFERENCE   1  (bases 1 to 2161)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 2161)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (26-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/12/2024 08:28:26
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 1,440
            CDSs (total)                      :: 1,416
            Genes (coding)                    :: 1,412
            CDSs (with protein)               :: 1,412
            Genes (RNA)                       :: 24
            rRNAs                             :: 1 (16S)
            partial rRNAs                     :: 1 (16S)
            tRNAs                             :: 21
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 4
            CDSs (without protein)            :: 4
            Pseudo Genes (ambiguous residues) :: 0 of 4
            Pseudo Genes (frameshifted)       :: 0 of 4
            Pseudo Genes (incomplete)         :: 3 of 4
            Pseudo Genes (internal stop)      :: 1 of 4
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2161
                     /organism="Candidatus Zixiibacteriota bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_1045059"
                     /isolate="SMAG_U2949"
                     /isolation_source="soil"
                     /db_xref="taxon:2053527"
                     /environmental_sample
                     /country="USA: Oregon"
                     /lat_lon="42.99 N 123.63 W"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            628..1302
                     /gene="cmk"
                     /locus_tag="VGB16_02265"
     CDS             628..1302
                     /gene="cmk"
                     /locus_tag="VGB16_02265"
                     /EC_number="2.7.4.25"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011025645.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004127 - cytidylate kinase activity
                     [Evidence IEA];
                     GO_process: GO:0015949 - nucleobase-containing small
                     molecule interconversion [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="(d)CMP kinase"
                     /protein_id="HEX9916536.1"
                     /translation="MSNVVIAIDGPAGSGKSTTAKLAANELGFVYLDTGAMYRAITLQ
                     CLRLRKNMNDEKEMTKIAKDSSIKFEFENGTSHVYLDGEDVTLDIRQPEIDKNVSILA
                     KHPKVREVLVAQQKNLSQKYNLVAEGRDTTTVVFPSAIKIYLTADLKERARRRLLELE
                     SKGIKSSLEEQIKEISRRDKLDSTRQASPLMLDKDAIVVDTTDLTISQQVERVVEIYK
                     EKMKTK"
     gene            1338..1910
                     /locus_tag="VGB16_02270"
     CDS             1338..1910
                     /locus_tag="VGB16_02270"
                     /inference="COORDINATES: protein motif:HMM:NF013702.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="lysophospholipid acyltransferase family protein"
                     /protein_id="HEX9916537.1"
                     /translation="MIVKVLRGFRVEGKENVPEKGGAIIASNHKSYVDPPVVTAAISR
                     ELYFLAKRELFGNKVFGWFISSLNAVPISRDKIDRKGLKGAIEILNQGNLLLVFPEGT
                     RSRKDDFLEPKLGVGKLALEAGVPIVPTLVSNTRHFLKNIFTGRKVTVNFGEPLNLNY
                     LQGFTEDKQGYQKIANEVMSRIKNLRKKAN"
     gene            complement(1921..>2161)
                     /locus_tag="VGB16_02275"
     CDS             complement(1921..>2161)
                     /locus_tag="VGB16_02275"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=2
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEX9916538.1"
                     /translation="CRRKAQARRDGEDIRLSGSTVLSGSPSKDSVEAHIEPSNPPFSK
                     GETSFPLFGKEARFARLAEAGRIEARARGDSLNVN"
BASE COUNT          651 a          413 c          539 g          558 t
ORIGIN      
        1 attaatgcgt ttgtattaaa ataactacca gcactatccc ctaaaacagg acatggaaag
       61 agggaaccgt ttgtccgaat tctggatctt gagacccgaa gtctgggtct tgagatggga
      121 taaggagaat cgagaattgg aaagtgtgcc agtggcttcg cccctaggcg agaaggcaat
      181 tttttgccct aaagatattc ctgtgttggg actgacccag aagtctattg tcatcgcgag
      241 cgaccaaggg gagcgtggcg atctcgattt tattatgtat aatattataa gagattgcca
      301 cgtcgtccgc cgcaggccct cggcttccct cggcttcgcc gagaggcgag cgccgagagg
      361 cgagcgatac tcctcgcaat gacagccaaa aaacgtttaa gtgaattaaa acaccctttt
      421 gggtcaaccc cctaaaagtg agggtgatga tttttttctt ttccctctcc ctggccgggc
      481 aggcactcta actctctccc ctaaggggag agagaaaccg gaggaaatag tgttaagaca
      541 ttctggcgtg tcttcaaaaa aaagttggga tgctcctgaa ataataaacc ttgataattt
      601 ggaatttttt ggttataata aaattttatg tctaatgtag ttattgccat agatggacca
      661 gctggctcag ggaaaagcac cactgccaag ctggcggcaa atgaattggg ttttgtctac
      721 ctggataccg gagcgatgta tcgggcaatt accctccaat gcctgcgttt gagaaaaaac
      781 atgaatgacg aaaaggagat gacaaagata gctaaagatt cttcaataaa gtttgaattt
      841 gagaatggaa ctagtcatgt ctatctggat ggggaggatg taactttaga tattcgccag
      901 ccggagatag ataaaaacgt ttccatttta gcaaagcacc ccaaagtcag ggaagtctta
      961 gttgctcaac agaagaacct aagccaaaaa tataacctgg tggcagaggg acgggataca
     1021 actactgtgg tgtttccatc tgccattaag atttatttaa ccgcagattt gaaagaaaga
     1081 gccagaagaa gacttctgga attagagtca aaggggataa agagttcttt agaggaacag
     1141 ataaaagaga tttcaagaag agacaaactc gattctactc gccaggcaag ccctcttatg
     1201 ttggataaag atgcgatagt ggtcgatacc acagatttga ccataagtca gcaggtggag
     1261 cgtgtagttg aaatttataa agagaagatg aaaactaaat gaggtggcat tacagatcag
     1321 tacggtactt ggtttacttg atagtaaaag ttttgcgggg atttagggtg gagggaaagg
     1381 agaatgttcc tgaaaaagga ggggcgataa tagcctcaaa tcacaagtct tatgttgacc
     1441 cgccagtagt gacagcggct atatcaagag aattgtattt tctggctaaa agagagcttt
     1501 tcggaaataa ggtttttggc tggtttatct cttccttaaa tgcagttcct ataagtcgtg
     1561 ataaaataga taggaagggc ttaaaaggag cgattgaaat cttaaatcaa gggaatcttc
     1621 ttttggtttt tccggagggg acgagaagca ggaaagatga ctttctggaa cccaaattag
     1681 gagtgggtaa gcttgctttg gaggccgggg ttccaattgt gccgacatta gtctctaaca
     1741 ccagacattt tttaaagaat atttttaccg gcaggaaagt tacagtcaat ttcggtgagc
     1801 ctttgaattt gaattatctc cagggtttta cagaagacaa acagggttat caaaaaatag
     1861 cgaatgaggt tatgtccaga attaaaaatt tgagaaagaa agctaactaa tacaaacgca
     1921 ttaatttacg ttcaatgaat ctcccctcgc cctcgcctcg attcggcccg cctcggcgag
     1981 acgggcgaag cgggcctctt ttccaaagag gggaaaagag gtctccccct ttgaaaaagg
     2041 gggatttgat ggttcgatat gggcttcgac tgagtccttc gacggtgagc cgctcaggac
     2101 ggtcgaaccg ctcagccgaa tgtcctcacc atctcttcga gcctgagcct tccttcgaca
     2161 c
//
LOCUS       DASWLN010000067         1756 bp    DNA     linear   ENV 20-FEB-2024
DEFINITION  MAG TPA_asm: Candidatus Zixiibacteriota bacterium isolate
            SMAG_U2949 k141_64966, whole genome shotgun sequence.
ACCESSION   DASWLN010000067 DASWLN010000000
VERSION     DASWLN010000067.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37960594
            Sequence Read Archive: SRR10376091
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Candidatus Zixiibacteriota bacterium (soil metagenome)
  ORGANISM  Candidatus Zixiibacteriota bacterium
            Bacteria.
REFERENCE   1  (bases 1 to 1756)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 1756)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (26-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/12/2024 08:28:26
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 1,440
            CDSs (total)                      :: 1,416
            Genes (coding)                    :: 1,412
            CDSs (with protein)               :: 1,412
            Genes (RNA)                       :: 24
            rRNAs                             :: 1 (16S)
            partial rRNAs                     :: 1 (16S)
            tRNAs                             :: 21
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 4
            CDSs (without protein)            :: 4
            Pseudo Genes (ambiguous residues) :: 0 of 4
            Pseudo Genes (frameshifted)       :: 0 of 4
            Pseudo Genes (incomplete)         :: 3 of 4
            Pseudo Genes (internal stop)      :: 1 of 4
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..1756
                     /organism="Candidatus Zixiibacteriota bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_64966"
                     /isolate="SMAG_U2949"
                     /isolation_source="soil"
                     /db_xref="taxon:2053527"
                     /environmental_sample
                     /country="USA: Oregon"
                     /lat_lon="42.99 N 123.63 W"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            <1..>1756
                     /locus_tag="VGB16_02280"
     CDS             <1..>1756
                     /locus_tag="VGB16_02280"
                     /EC_number="2.2.1.1"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011523039.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=2
                     /transl_table=11
                     /product="transketolase"
                     /protein_id="HEX9916539.1"
                     /translation="YLSLGMSGYFDIEEVVTLRKLYSPFQGHPHWLKLPGIEASTGSL
                     GQGLSIANGIALAGKLDNKDYHVFALTGDGEHQEGQVWEAIMEAGHYKLDNLTCILDK
                     NRLQIDGWVEEVMNIDPIVEKYKAFNWNVIEIDGHNMKEILDAFEKAKSLKGKPTVII
                     AHTIKGKGVSFMENVAGWHGKSPVCEQMDKALEELGLKGNLDRDRLFKKAEDYQTVAS
                     QKLESKMPKFKKVYFWNKQPNMKVKMDPTRMGFGRALDEKGDDKRIVCLGADISGSIA
                     ISHFYEKHPERKNRFLSMGIAEQSGTGVAAGLAKEGKIPVFGTYGVFAAGRNLDQLRT
                     TVCYGNFNVFIAGAHGGVSVGPDGATHQALEDLFQMCGLPNMNVVAPCDSIETKKATE
                     FLLFDVKGPKFLRFAREATPVVTFTDTPFVFGEANIIRYRGEEEEEFIHAFETKLSSQ
                     YKSEHEDLTIIACGPEVPEAMRAAWILKEDFGIESRIVNLHTLKPLDKKTIIKAALET
                     GVVITAEEHQIGGLGNWIASSILEAEEVYQKPILFGMVGVKDRFGESGQPWELIKEFE
                     VSAEHIAAKAKELYDRKKA"
BASE COUNT          543 a          305 c          469 g          439 t
ORIGIN      
        1 atatttaagc ctggggatgt caggttattt tgatatagaa gaggtggtga cattaagaaa
       61 actctattcc ccttttcaag gacaccctca ctggctgaaa ctacccggaa ttgaagcttc
      121 tactggctct ttagggcagg ggttaagtat tgctaatggt atcgccttag caggtaaact
      181 agataacaag gattatcacg tttttgcttt aaccggagat ggtgaacatc aggaagggca
      241 ggtctgggaa gcgataatgg aggctgggca ctataaatta gataacctga cctgtatctt
      301 ggacaaaaac cgcttgcaga tagatggctg ggtagaagag gtgatgaata tagacccgat
      361 tgtggagaaa tataaagctt tcaactggaa tgtcatagag atagatggcc ataatatgaa
      421 agagatattg gatgcttttg agaaggcaaa atctctcaag gggaagccca cagtaataat
      481 tgctcatacc attaagggta aaggggttag ttttatggag aacgtggccg ggtggcatgg
      541 gaaatcgcca gtgtgtgagc agatggataa agccttagaa gagttggggc ttaaagggaa
      601 cctggatcgg gatagattat tcaaaaaagc tgaggattat caaaccgtag cctctcagaa
      661 actggaatcg aaaatgccga agttcaaaaa agtctatttc tggaacaaac agccgaatat
      721 gaaagtgaag atggacccta ccaggatggg ttttggcaga gctttagatg agaaagggga
      781 tgataaaagg atcgtctgtt taggtgcgga tatttcaggc tcaattgcca taagccattt
      841 ctacgagaaa catcctgaaa gaaaaaatag atttttgagt atgggaattg ctgaacagag
      901 cggaaccggc gtagcagctg gtttagcaaa agaagggaaa atcccggttt tcggtaccta
      961 cggcgttttt gccgcgggca ggaatttaga ccagctgaga actacagtct gttatgggaa
     1021 cttcaacgtg ttcatagctg gcgcgcacgg tggggtttca gttggtccgg atggtgccac
     1081 ccatcaggcg ttagaagacc ttttccagat gtgcgggctt cccaatatga atgtggtagc
     1141 tccctgtgat tcgatcgaga ccaaaaaagc tactgaattt ctcttattcg atgttaaagg
     1201 gccaaagttc ttgagatttg ccagagaggc aactcctgta gttaccttta cggatacacc
     1261 ttttgtattc ggagaggcaa atataatcag gtatagggga gaggaggaag aggagtttat
     1321 tcatgccttt gagacgaaac tttcctcaca gtataaatct gaacatgaag atttgaccat
     1381 aatcgcctgc ggaccagaag tacctgaggc aatgagagca gcctggattt taaaggagga
     1441 tttcgggatt gagtccagaa tagtgaattt acatacctta aaacctctgg ataagaaaac
     1501 aattatcaaa gcagccttag aaaccggagt tgtcattact gcagaagaac atcagatcgg
     1561 cggtttaggg aactggattg cctcttcaat ccttgaagca gaggaggttt atcagaaacc
     1621 gatattgttc gggatggtcg gagtgaaaga ccggtttggc gaatccggac aaccctggga
     1681 gctgataaag gaattcgagg tttcagcaga acatattgcg gcaaaagcca aagagcttta
     1741 cgatagaaaa aaggct
//
LOCUS       DASWLN010000068         6804 bp    DNA     linear   ENV 20-FEB-2024
DEFINITION  MAG TPA_asm: Candidatus Zixiibacteriota bacterium isolate
            SMAG_U2949 k141_317596, whole genome shotgun sequence.
ACCESSION   DASWLN010000068 DASWLN010000000
VERSION     DASWLN010000068.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37960594
            Sequence Read Archive: SRR10376091
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Candidatus Zixiibacteriota bacterium (soil metagenome)
  ORGANISM  Candidatus Zixiibacteriota bacterium
            Bacteria.
REFERENCE   1  (bases 1 to 6804)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 6804)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (26-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/12/2024 08:28:26
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 1,440
            CDSs (total)                      :: 1,416
            Genes (coding)                    :: 1,412
            CDSs (with protein)               :: 1,412
            Genes (RNA)                       :: 24
            rRNAs                             :: 1 (16S)
            partial rRNAs                     :: 1 (16S)
            tRNAs                             :: 21
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 4
            CDSs (without protein)            :: 4
            Pseudo Genes (ambiguous residues) :: 0 of 4
            Pseudo Genes (frameshifted)       :: 0 of 4
            Pseudo Genes (incomplete)         :: 3 of 4
            Pseudo Genes (internal stop)      :: 1 of 4
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..6804
                     /organism="Candidatus Zixiibacteriota bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_317596"
                     /isolate="SMAG_U2949"
                     /isolation_source="soil"
                     /db_xref="taxon:2053527"
                     /environmental_sample
                     /country="USA: Oregon"
                     /lat_lon="42.99 N 123.63 W"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            227..1408
                     /locus_tag="VGB16_02285"
     CDS             227..1408
                     /locus_tag="VGB16_02285"
                     /EC_number="5.4.2.7"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_013822978.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0000287 - magnesium ion binding [Evidence
                     IEA];
                     GO_function: GO:0008973 - phosphopentomutase activity
                     [Evidence IEA];
                     GO_process: GO:0009117 - nucleotide metabolic process
                     [Evidence IEA];
                     GO_process: GO:0043094 - cellular metabolic compound
                     salvage [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="phosphopentomutase"
                     /protein_id="HEX9916540.1"
                     /translation="MPEINRVILIILDSCGVGELPDAEEYNDQGSNTIGNTASAVKGL
                     FLPHLQELGIGNIINIEGVGPEEYPLANYGKMAERSIGKDSTTGHWEIAGLIVNKPFP
                     TYPQGFPTEIIKEFEKTIQRQILGNKPASGTEIIKELGEEHLKTGKPICYTSADSVFQ
                     IACHEDVLSPEELYEICQKARGILTGEHGVARVIARPFIGKPGSFKRTERRKDFSLSP
                     PGETILDILKKNRLQVIGIGKIEDLFASRGLTESLHTENNLDGIAKLYQKMEEHFTGL
                     SFINLIDFDMLWGHRNDVVGFARGLEEFDLALAEILGLLKSDDLLIITADHGCDPTTS
                     STDHSREYVPLLVYGEMLKQGVDLKTRETFSDIAQTLAEIFNVGGTGNGRSFLKEIYV
                     R"
     gene            1398..1844
                     /gene="cdd"
                     /locus_tag="VGB16_02290"
     CDS             1398..1844
                     /gene="cdd"
                     /locus_tag="VGB16_02290"
                     /EC_number="3.5.4.5"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011878788.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0005737 - cytoplasm [Evidence IEA];
                     GO_function: GO:0004126 - cytidine deaminase activity
                     [Evidence IEA];
                     GO_process: GO:0008655 - pyrimidine-containing compound
                     salvage [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="cytidine deaminase"
                     /protein_id="HEX9916541.1"
                     /translation="MSDEELIEKAKATRGNAYAPYSQFKVGAAILGKSGKVYTGANVE
                     NSSYGLTVCAERVALYCAVSQGEREFVKLALVTDREEPAIPCGACCQALSEFNPDMEI
                     ICANLQGITKKITLKRLLPNFFRIESPPTGRGFKPEPNRDPEASSE"
     gene            complement(1920..2048)
                     /locus_tag="VGB16_02295"
     CDS             complement(1920..2048)
                     /locus_tag="VGB16_02295"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEX9916542.1"
                     /translation="MNGDERCICWKQKKIDQMQNKQAYPVVRPEMLKRVDAETSLP"
     gene            2105..2788
                     /gene="deoC"
                     /locus_tag="VGB16_02300"
     CDS             2105..2788
                     /gene="deoC"
                     /locus_tag="VGB16_02300"
                     /EC_number="4.1.2.4"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_014067379.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004139 - deoxyribose-phosphate aldolase
                     activity [Evidence IEA];
                     GO_process: GO:0009264 - deoxyribonucleotide catabolic
                     process [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="deoxyribose-phosphate aldolase"
                     /protein_id="HEX9916543.1"
                     /translation="MDLCPKDMARFIDHTLLKPEATEKDIRKLCEEAKGYGFASVCAN
                     PIYVTLCQGLLRGSNTKTCTVVDFPLGANKTEVKVLQTKLACQDGAEELDMVANLGAL
                     KAGDLKLFEKDIKAVVDAKPEGVILKVIIETAILTDNEKKTAASIVKECGADFVKTST
                     GFGPAGANVGDIALLRKVVGEGIGVKASGGIRTYAQVLEMIKAGANRIGTSASVQIME
                     SYATLSDHI"
     gene            2763..4088
                     /locus_tag="VGB16_02305"
     CDS             2763..4088
                     /locus_tag="VGB16_02305"
                     /EC_number="2.4.2.4"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011344461.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_process: GO:0006206 - pyrimidine nucleobase metabolic
                     process [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="thymidine phosphorylase"
                     /protein_id="HEX9916544.1"
                     /translation="MLPYQIISKKRDRNKLSREELEFFLHGFVKGEIPDYQMSALLMA
                     IFLNGMDEQETLDLTLCLVRSGKILNLKSIPGIKVDKHSTGGVGDKVSLVLAPWVASC
                     GVVVPLISGRGLGHTGGTLDKLSSIPGFKTDISLKRFVSLLEANGLAMIGQTKQIAPA
                     DGKLYALRDVTATVNSIPLIAASIVSKKLAVNSDAIVFDVKVGNGAFVSSREDGLKLA
                     HILVRICHKMNKKSIALITDMNEPLGHMVGNSLEVIEAIEYLKGNGPEDLVDVCLGLG
                     SHMLILGKRAKSQTEAIDILKKAMQNGSALKKFKQMVRAQNGNERVIDDYSLLPQAKY
                     VIEVKSTASGWVKSIDTSSIGLLGVEIGVGRKKMEDRIQLGVGFSIKTKVGDYIEKGQ
                     ALAKIFINDLKKGKEISHRLRQAFVLSSRPVRKLKKILYLVNNRGAREL"
     gene            complement(4109..6271)
                     /locus_tag="VGB16_02310"
     CDS             complement(4109..6271)
                     /locus_tag="VGB16_02310"
                     /inference="COORDINATES: protein motif:HMM:TIGR02074.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008658 - penicillin binding [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="PBP1A family penicillin-binding protein"
                     /protein_id="HEX9916545.1"
                     /translation="METREDLTPEESTYHVRRKRTVFLVSLSAILVLIILLFLELLNI
                     YNKGLPSFSQLTNIEPSLSTKIYSADGNLIKEFYTQRRILVPLKQMPPYLVNALLASE
                     DRKFYSHWGIDLTGTARALTTAILRLRNIQGASTITQQLARTLFLNLDKTMSRKIKEA
                     LTAIKLERTYSKDDILEMYLNQIYFGEGAYGVQAAAQVFFSKDAKELSLPECALLVGV
                     LPSPNRYSPFKDPDLALSRRNSVLKAMKDFGKLTKAQYDSLKALPLQFKPSSLRMGSA
                     PYFTEMVRQYLEKQYGEKDLYSSGLVVYTTLNFKLQEEAEKIVLEQLDVLQQQMEKTH
                     SLDDTNYTVEVVGSLAPVKDKNTKREYKQMQGAVLAIDNNTGDILALVGGKDFEKSKF
                     NRAIQAQRQPGSAFKPFVFTAAIDNGYKVTDIIYDTPIVLIGGDGKEWRPENYDKTFR
                     GPMNLREALRISRNLVSIKLLQKIGPEQAVFYASKMGIKSPLQAIPSLAIGTSEVNLL
                     ELTSAFSVFPNAGIKVEPRYITKIVDRYSNILEESPPPQKEEVLSVQTSCIMTSLLQT
                     VVDRGTGAAARAMGFSRPAGGKTGTTDECMDNWFVGFTPQITTGVWVGYDDKTVIGKD
                     VTGATTALPIWTNFMLKAHEGLPVENFQEPTGIVHKIVCTESGLLATDKCPSMVEEIF
                     VQGNEPEKSCDIHPSKNPPSPDKIKSRTEQKKKEELHF"
     gene            6388..>6804
                     /gene="tyrS"
                     /locus_tag="VGB16_02315"
     CDS             6388..>6804
                     /gene="tyrS"
                     /locus_tag="VGB16_02315"
                     /EC_number="6.1.1.1"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_018206267.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0005737 - cytoplasm [Evidence IEA];
                     GO_function: GO:0004831 - tyrosine-tRNA ligase activity
                     [Evidence IEA];
                     GO_process: GO:0006437 - tyrosyl-tRNA aminoacylation
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="tyrosine--tRNA ligase"
                     /protein_id="HEX9916546.1"
                     /translation="MKNIKEQLEVIKRGAVEVIPEEELVKKLERSIKANTPLRVKQGF
                     DPTAPDIHLGHTVGLRKLKEFQELGHEIVLIVGDYTAMVGDPSGRSETRPQLSLAQVK
                     KNAETYQKQFFKVVAKKRTEVRFNSEWFSRMSFLEIM"
BASE COUNT         1984 a         1360 c         1646 g         1814 t
ORIGIN      
        1 aaaccaagga gaaaaaaggg aaagcagaga agaaggaagc aactcccaaa aaggagaaag
       61 ctaagaagaa accatcgccc aagaaagagg aagataagac cagcgagaaa gaaaaggaag
      121 ataccgagta atccataaag acctttaaag gcagtccatc tggactgcct tttttgtttg
      181 ttttcccttt acaattgatt ttggttagat tatcataaaa ataaatatgc cagaaataaa
      241 tcgtgtcatc ttaattatct tagatagttg tggagtgggc gagttaccgg atgccgagga
      301 gtataatgac caagggtcaa ataccattgg caacaccgcc tcagcggtga agggactttt
      361 tcttccccat ttgcaagagc tgggaattgg aaatataatc aacatcgagg gagtaggccc
      421 ggaggaatat cctttagcca actatggcaa gatggctgaa agatcaattg gcaaagatag
      481 caccactggc cattgggaga tagccggttt aattgtaaat aaaccgtttc ccacgtatcc
      541 ccaagggttc cccacggaaa tcataaagga atttgagaaa acgatccaga gacaaatctt
      601 aggcaataag cccgcttctg gaaccgagat tattaaagaa ttaggggaag agcatctcaa
      661 aaccggcaaa cctatttgct atacatcagc agatagcgtt tttcagatcg cgtgccacga
      721 agatgtgtta tcgccagaag agctttacga aatatgccaa aaagcaagag ggatattgac
      781 cggcgaacat ggagttgcaa gagtcatagc tcgacccttt attggcaagc caggttcgtt
      841 caaaagaacc gagagacgca aagatttttc cctttcacct ccaggtgaga ctattctgga
      901 tatcctcaag aagaacagac ttcaagtaat tggaattggt aaaattgaag acctttttgc
      961 aagcaggggc ttaactgaat cgcttcacac tgaaaataat ctggacggca tagctaagct
     1021 atatcagaag atggaagaac attttaccgg tttaagtttt attaatttaa tagatttcga
     1081 tatgctgtgg gggcaccgca atgatgtggt tggttttgct cgcggattag aagaattcga
     1141 tttagcattg gcagagattt taggtttgct aaagtccgat gacctgctta tcatcactgc
     1201 tgaccacggc tgtgacccta ccacttccag caccgaccac tcccgagaat acgtcccttt
     1261 gttagtgtac ggtgagatgc tcaagcaggg cgtggatttg aaaaccagag agacattttc
     1321 tgatatagct caaaccttag ccgagatatt caatgttggg ggaactggaa atggaagaag
     1381 tttcctgaag gagatttatg tcagatgaag aactcataga aaaggcaaaa gcaacaagag
     1441 gtaatgccta tgctccctat tcgcaattca aagttggggc agctatctta ggaaagagtg
     1501 gaaaagttta taccggggca aatgtggaga atagctctta tgggttaacc gtctgcgctg
     1561 agagagtggc gctttattgt gcggtgagtc agggtgaaag ggaattcgtt aagctggctt
     1621 tggtcacaga cagagaagag ccagctatcc cctgtggggc ttgctgtcaa gcgctatccg
     1681 aattcaatcc ggatatggag ataatttgtg ccaatctgca aggcattacg aagaaaatta
     1741 ccctgaaaag gcttttgccc aactttttca gaatagagag cccgcccaca gggcggggct
     1801 ttaaacccga accaaaccga gatcccgaag caagttcgga atgacaaaaa ccgtaagacc
     1861 aaacccggtt gtcatgctga accctgaaag tgtcatgctg aacttggttc agcatctctt
     1921 cagggcaggc tcgtttcagc atctactcgt ttcaacatct ctggtctgac tacaggatag
     1981 gcttgtttgt tttgcatctg gtctattttt ttctgcttcc aacagatgca cctttcgtcg
     2041 ccgttcacag gagaggggtt tttcgggtgc gatcttaaat aaagtaaaag gagttggtga
     2101 aatgatggat ttgtgcccaa aggatatggc aagattcata gaccatacat tgcttaaacc
     2161 ggaggcaacg gaaaaagata taagaaaact gtgtgaggaa gcaaaaggat atggttttgc
     2221 cagcgtttgc gctaacccca tttatgttac cctctgccag gggcttctca gaggctcaaa
     2281 cactaagacc tgcaccgtgg ttgattttcc gttgggtgct aataaaaccg aggtcaaggt
     2341 tcttcagaca aagttggctt gtcaggatgg agcagaagag ctggatatgg tggctaacct
     2401 cggggcatta aaagctggag atttaaaact gtttgagaag gatataaaag ctgttgtgga
     2461 tgctaagcca gaaggtgtca tcttgaaagt aataattgaa acggcaatat tgacagataa
     2521 tgaaaagaaa acagctgcta gcatagtcaa ggagtgcgga gccgattttg ttaaaacctc
     2581 caccggcttt ggtccggctg gagcgaatgt tggtgatatc gctttgctaa gaaaagtagt
     2641 aggagaaggg attggagtca aagcctcagg tggaataaga acctatgctc aggtcttgga
     2701 gatgataaaa gccggtgcga atcgcatcgg cacttcagcc agtgtgcaaa taatggaaag
     2761 ttatgctacc ctatcagatc atatctaaga aaagagatag aaataagcta agcagggaag
     2821 agctggagtt tttcctccac ggattcgtca agggtgaaat tcctgactat cagatgtcag
     2881 ccctgcttat ggcgattttc ttgaatggga tggatgagca ggaaactctt gacctcacgc
     2941 tttgtctcgt gcgttccgga aagattctga atttgaaatc aattcccgga attaaagtag
     3001 ataaacatag caccggtggg gtgggagata aggtctcctt ggttttagcc ccttgggtag
     3061 catcctgtgg cgttgtggtt cctttaatat ccggcagagg tttgggtcat actggcggca
     3121 ccctggataa gttaagctcg atacccggtt tcaagacaga tatttcctta aagagatttg
     3181 tctccttgct ggaggcaaat ggcttagcta tgattggcca gaccaaacaa atcgctccgg
     3241 ctgatggcaa actctacgct ttaagggatg ttaccgccac ggtaaattca attcccctga
     3301 ttgcggctag cattgtcagt aaaaaattgg cggttaactc cgacgccatt gtttttgacg
     3361 tgaaggttgg aaatggggct tttgtgtcat ctcgagagga tggtttgaaa ttggcacata
     3421 tcttggttag aatttgccat aagatgaaca agaaatcaat tgccttgata actgatatga
     3481 acgaaccttt gggtcatatg gtgggcaatt ccctggaggt aatagaagcg attgagtact
     3541 taaagggaaa tggacccgaa gatttagttg atgtctgttt gggtttgggc tcacacatgc
     3601 ttatcctggg caaaagagca aaaagccaaa ctgaagcgat tgacatcttg aagaaagcga
     3661 tgcaaaacgg ttcagcccta aagaaattca agcagatggt gagagctcag aatggtaacg
     3721 aaagagttat tgacgattac agtcttctgc cccaggctaa gtacgtcata gaagtgaagt
     3781 caaccgcttc aggttgggtg aaatcaattg atacttcaag catcggactt ttgggagtgg
     3841 aaataggtgt gggtagaaag aaaatggaag acagaattca attgggagta ggattctcga
     3901 taaagacaaa agtcggtgac tacatagaaa agggacaggc actcgctaag atattcatca
     3961 acgatttgaa aaaagggaag gagattagcc acagattaag acaggcgttt gtgttaagct
     4021 caaggccagt caggaaactc aagaagattc tctatttggt taataacagg ggtgcaagag
     4081 agttgtaggc ctaacgcatt taagctactc agaaatgtaa ttcctctttc ttcttctgct
     4141 ccgtgcggga ctttattttg tcgggtgagg gagggttttt ggaagggtga atgtcgcagc
     4201 ttttctccgg ttcattccct tgaacaaaga tttcctcaac catggaaggg catttatcag
     4261 tagccaaaag tccactttca gtgcagacga ttttgtgaac tatgcctgtc ggctcttgga
     4321 agttctcgac aggtaatccc tcgtgtgcct ttagcataaa gtttgtccag attggcagag
     4381 cggtagttgc accagtaaca tcttttccga ttaccgtctt atcatcatat cccacccata
     4441 ccccggttgt tatctgtgga gtaaacccaa caaaccagtt gtccatacat tcatccgttg
     4501 tgccggtttt tccaccagcc ggtcgactaa atcccatagc tctggctgcg gctcctgtgc
     4561 cacggtccac cacagtctga agcaaactgg tcataataca agatgtttgg acactcaaca
     4621 cctcttcttt ttgaggaggg ggactttctt cgagaatatt gctgtaacgg tcaacaattt
     4681 ttgtgatata tctcggctcc accttgatgc cggcattggg aaaaacagaa aaagccgagg
     4741 tcaattcaag taaattaacc tcggaagttc caatagctaa agaggggata gcttggagtg
     4801 gggatttgat tcccatcttg gaagcataga atacggcctg ctccggacca atcttctgca
     4861 atagttttat gctgactaaa tttctggaga tgcgcagggc ttcccttaag ttcatcggac
     4921 ccctgaaggt cttgtcgtaa ttctccggtc tccattcctt gccatcgccg ccaatgagca
     4981 cgataggggt atcgtagatt atatctgtta ccttgtatcc attatcaata gcggcagtga
     5041 agacaaaggg cttgaaggct gacccgggct gtctttgggc ttgaatcgct cggttgaact
     5101 tgcttttctc gaagtccttt cctccgacca gagccagaat atccccggtg ttattatcaa
     5161 ttgccaacac cgccccttgc atttgtttat attctctttt tgtattttta tctttgaccg
     5221 gggcaagcga gcccacaacc tccacagtat agtttgtgtc atccagagaa tgagtttttt
     5281 ccatctgctg ctggaggaca tccagttgct ccagcactat tttttcggct tcttcctgca
     5341 acttgaaatt cagagtagtg taaaccacta gcccgctgga ataaaggtct ttttctccat
     5401 attgtttctc caagtactgc ctcaccatct cagtgaaata gggagccgaa cccatcctca
     5461 aagaggaagg tttgaactga aggggtaacg ctttcaagct atcgtactgg gctttggtca
     5521 actttccgaa atctttcatc gctttcagaa ctgaattcct acgacttaaa gctaaatctg
     5581 gatccttaaa aggggaataa cgattggggg atggcaacac ccctaccaag agggcgcatt
     5641 caggcaaact taattccttg gcatccttgc tgaagaaaac ctgagccgcg gcttgtacgc
     5701 cataagctcc ttctccgaaa taaatctgat ttaaatacat ctctaaaatg tcatccttgg
     5761 aataggtcct ttcaagtttt atagcagtta atgcctcctt gattttccga gacatagttt
     5821 tatccaaatt caagaagagc gttcgagcta actgctgagt tatggtggag gcgccctgga
     5881 tgttcctgag ccgtaagatg gcagttgtca atgcgcgggc tgttccggta aggtcgattc
     5941 cccaatgaga atagaatttt ctatcttcag acgccaaaag ggcgttcacc aaataggggg
     6001 gcatttgctt taaggggact aagattctcc tctgagtata gaattccttg attaggttcc
     6061 catcagcgga atagatttta gtggataagc tcggctcgat attggtcagc tgtgaaaatg
     6121 acggcagtcc tttattgtaa atgttcagga gttcgagaaa taaaaggata atcagaacaa
     6181 gaatcgcaga cagagagacg agaaaaacag tcctttttct tcttacatga taagtgcttt
     6241 cttcaggggt caaatcttct cgggtttcca taatatgagt caattaactt ctttcgattt
     6301 tggtgtcaag ctaaatttag cttgattttt gaagagataa agttatcata taatacaacg
     6361 aataaaacaa taatttcatt tggaaatatg aagaacatta aagaacaatt agaagtcatc
     6421 aaaaggggtg cagtagaggt tatccccgag gaagagcttg tcaaaaagct tgaaaggtca
     6481 atcaaggcca atactccact tcgggtgaag cagggttttg accccactgc tccggatata
     6541 catctgggac atactgtggg attaagaaaa ctgaaggaat ttcaggaatt gggacacgaa
     6601 atagtcttaa tcgtagggga ttatactgct atggtggggg acccgagcgg acgctctgaa
     6661 acccgacccc aactctccct ggctcaagtg aagaaaaatg cggagaccta tcaaaagcag
     6721 tttttcaagg tagttgccaa aaagaggacc gaagttcgtt tcaatagtga atggttcagt
     6781 cggatgagtt ttctggaaat tatg
//
LOCUS       DASWLN010000069        12633 bp    DNA     linear   ENV 20-FEB-2024
DEFINITION  MAG TPA_asm: Candidatus Zixiibacteriota bacterium isolate
            SMAG_U2949 k141_66482, whole genome shotgun sequence.
ACCESSION   DASWLN010000069 DASWLN010000000
VERSION     DASWLN010000069.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37960594
            Sequence Read Archive: SRR10376091
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Candidatus Zixiibacteriota bacterium (soil metagenome)
  ORGANISM  Candidatus Zixiibacteriota bacterium
            Bacteria.
REFERENCE   1  (bases 1 to 12633)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 12633)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (26-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/12/2024 08:28:26
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 1,440
            CDSs (total)                      :: 1,416
            Genes (coding)                    :: 1,412
            CDSs (with protein)               :: 1,412
            Genes (RNA)                       :: 24
            rRNAs                             :: 1 (16S)
            partial rRNAs                     :: 1 (16S)
            tRNAs                             :: 21
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 4
            CDSs (without protein)            :: 4
            Pseudo Genes (ambiguous residues) :: 0 of 4
            Pseudo Genes (frameshifted)       :: 0 of 4
            Pseudo Genes (incomplete)         :: 3 of 4
            Pseudo Genes (internal stop)      :: 1 of 4
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..12633
                     /organism="Candidatus Zixiibacteriota bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_66482"
                     /isolate="SMAG_U2949"
                     /isolation_source="soil"
                     /db_xref="taxon:2053527"
                     /environmental_sample
                     /country="USA: Oregon"
                     /lat_lon="42.99 N 123.63 W"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            <1..1779
                     /locus_tag="VGB16_02320"
     CDS             <1..1779
                     /locus_tag="VGB16_02320"
                     /EC_number="2.7.6.5"
                     /inference="COORDINATES: protein motif:HMM:TIGR00691.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_function: GO:0005525 - GTP binding [Evidence IEA];
                     GO_function: GO:0046872 - metal ion binding [Evidence
                     IEA];
                     GO_process: GO:0015970 - guanosine tetraphosphate
                     biosynthetic process [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="bifunctional (p)ppGpp
                     synthetase/guanosine-3',5'-bis(diphosphate)
                     3'-pyrophosphohydrolase"
                     /protein_id="HEX9916547.1"
                     /translation="LLSMAKDIRVIIIKLADRLHNMRTLDSLNPEKQKRIAQETRDIY
                     APLAHRFGMARIEWELEDLSLRYLEPEVYGELLKKVEEKREDREAYIQEISQPLQREL
                     GKAGIKAKITGRAKHFYSIYRKMKKRNKPFEEIYDLFAIRVIVSTEAECYHVLGIVHT
                     LWKPVEDRFHDYIALPKSNMYRSLHTTIIGPRGRMVEIQIRTHEMHHAAEYGIAAHWL
                     YKEGKRKLDDTDKQMIWLREVLEWQKDLTNPKEFLEYLKVDLFHDDIFVFTPKGELRQ
                     LPRGSTPLDFAYTVHSDIGDHCVGVKVNGRMVPLSTVLNSGDEVDIVTSSHQAPSQDW
                     LKIVKTAKARSRIKQWLKQKGYEQGMALGKEIFEKELKKQHLPLPSEEELIDLAMSLN
                     YSSLSAMFYALGSGGISMHQILGKLVPEKKEIKESLVEKLIHKAKGVGKGIRVQGEGN
                     MMFRFAQCCQPLPGEKIMGFITRGRGVSIHRADCTNVLQTMGNSDRQVEVQWDVGKDE
                     SFVVRLELVLEDRKNMLRDITEAITDADANVRGAEIKSGETMAMGDFLIEIRNLNHLN
                     RTIKKIKEVKGVVAVKRAEGVEFPHE"
     gene            1772..2524
                     /gene="pgeF"
                     /locus_tag="VGB16_02325"
     CDS             1772..2524
                     /gene="pgeF"
                     /locus_tag="VGB16_02325"
                     /inference="COORDINATES: protein motif:HMM:NF014619.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="peptidoglycan editing factor PgeF"
                     /protein_id="HEX9916548.1"
                     /translation="MNKEWNKLDNGQINWLEFSKLKNIPFVSFGLVVKNCKGKSFAIE
                     VCWKDKFKRFLRKQIGYAGGIVVPRQVHQNNVASITKSEKFNLRKKLQADALLTNQKG
                     LFLTIQVADCIPIYIVDTKKKVVGLVHAGWRGTLMQIAKDVVGEARRSFNCSPEDLKI
                     IFGPAIEKCCYEVKPEVAILFPPPFVNYEGDKRPKLDLAGFNRAQLIESGVKKKNISL
                     AGECTFCGPKFLCSYRRGRNKKERMLAFIGIK"
     gene            2617..3243
                     /locus_tag="VGB16_02330"
     CDS             2617..3243
                     /locus_tag="VGB16_02330"
                     /inference="COORDINATES: protein motif:HMM:NF024895.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="outer membrane beta-barrel protein"
                     /protein_id="HEX9916549.1"
                     /translation="MKKVFLLLAVFFLLVSPSYAFTGLGLGVRAGIVSNYDNPTLDEL
                     FDAPFVLKGKKIEDMNMLGAHLKIGTLPIIDLEISGEYFWKTEEFSFEFVDYQNPSGV
                     DTANGEFKIEDYSVNVTAKYVFPTSTIKPYVGGGGGLHIIKYSNPGPGPFFENQSKPS
                     YHACGGVLLDVPVLPLEVFLEGRYTSIQTEKKATGFFTWMVGVTLNLP"
     gene            3568..4212
                     /locus_tag="VGB16_02335"
     CDS             3568..4212
                     /locus_tag="VGB16_02335"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEX9916550.1"
                     /translation="MKKIFAFFVVVFFILTSGCLAQFSGQLSTAKVTKEGGVLGGGYI
                     GIYEDAFSIFGQVRYGFITDLDGGFKLGILNLDPGNGFGESETGVILGGDVKYQILNL
                     KAEDPLDFSFGFGMEYLNVSDYSLFSFGGNLVGSYDFELKSKKVVTPYGRLNLRGERV
                     EVSIPGWTGSDDTDFDIAFSLGCNLQMTSELSMIAELQIDDNVGVIGGISYYIF"
     gene            4278..5093
                     /locus_tag="VGB16_02340"
     CDS             4278..5093
                     /locus_tag="VGB16_02340"
                     /inference="COORDINATES: protein motif:HMM:NF014703.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="undecaprenyl-diphosphate phosphatase"
                     /protein_id="HEX9916551.1"
                     /translation="MELTKAIFLGIVQGLTEFLPVSSSGHLVIVEHILKVKMPGVSFE
                     VCLHLGTFFSIVVIYWGKIVKMIKATILKACSLFSGGFDKALEEDWKLSYSIVLGTIP
                     AGILGVALKHQIENAFTSISLVAVMLGITGVILWLSGSAKTKKDNAGLLDTIVIGIAQ
                     GFALLPGISRSGMTISAGMARGIDKPKAAEFSFLLSLPAILGASVLEFSNLAVERKFL
                     FSNIDFYIVGFLTAFVFGYIAIRFLLRVIQKGKFRYFGFYCLAVAILVLLFVR"
     gene            5090..6124
                     /locus_tag="VGB16_02345"
     CDS             5090..6124
                     /locus_tag="VGB16_02345"
                     /EC_number="2.4.-.-"
                     /inference="COORDINATES: protein motif:HMM:NF013257.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016757 - glycosyltransferase activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="glycosyltransferase family 9 protein"
                     /protein_id="HEX9916552.1"
                     /translation="MTKDIKKILIIRLSSLGDVVLTTPVIKALKDKLPQAEIHFLTKS
                     KYASLLKDNPHLSSIMELEDRGVSGLLFTLRKIRKLNFDLVIDLHANLRSFFIRRLSR
                     AKLKIRYNKRWIARFLMVNFKKIKIPSQHTVDSYLSCLKRLDIHISNRMPELYLDEKS
                     KEFAEQLLTGVSKDDILIGIVPGAKWETKKWGEENFAKAIEILNNRIKASFLVFGAKE
                     DEVLISKLKSLAGDIDFMEIIGLPFPKLSALISRCGIILTNDSGPMHIAVALKVPVLA
                     IFGPTHPKLGFSPLGERDTILCANVECSPCSLHGERECYQKTKICMEKITPEMVADEV
                     LEILKDREKI"
     gene            6206..6388
                     /locus_tag="VGB16_02350"
     CDS             6206..6388
                     /locus_tag="VGB16_02350"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEX9916553.1"
                     /translation="MKYMTTKLQSEVETVLLYNYALLRRLKLDEWEEEIRKRLREFVS
                     LRKDRQIYSSKLKEEE"
     gene            6438..7343
                     /locus_tag="VGB16_02355"
     CDS             6438..7343
                     /locus_tag="VGB16_02355"
                     /inference="COORDINATES: protein motif:HMM:NF015948.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF362 domain-containing protein"
                     /protein_id="HEX9916554.1"
                     /translation="MSKSRVIVVRHTRVSDKSGRVDSNVLSSMLSAGILKLTQEKDTS
                     DGLSHFFNPTDHIGMKTNCLSGKNSSTHPELAYAISSMLGKSGVKNKNIFIWDRSNRE
                     LKEAGFKINTSGDGPRCFGTDSYGVGYAEKLYTYKNIGSLFSRIFIDFTDTQINLPVL
                     KDHTLAGITCGMKNLFGIIHNPNKYHSNNCDPYVADLSSMPLVKNKIKLIICDCIGVQ
                     YNAGPSFHPQWWEKYDGILMGTDQVAVDFVGYTILDQIRAKSGLPPLKKIGEEPKYIF
                     TAADVEHNLGNVDLKNIDLVELKLD"
     gene            7414..8553
                     /gene="amrS"
                     /locus_tag="VGB16_02360"
     CDS             7414..8553
                     /gene="amrS"
                     /locus_tag="VGB16_02360"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_006927503.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0051536 - iron-sulfur cluster binding
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="AmmeMemoRadiSam system radical SAM enzyme"
                     /protein_id="HEX9916555.1"
                     /translation="MFRLSRREFLKSSALAGATSLLPFGSILNLSQEALASERDENLS
                     WVEAKYYDKLQNREIQCQLCPRECRVGDRERGYCGVRENRDGIYYTLVYSKVCSANVD
                     PIEKKPFFHFLPGTEAFSIATAGCNLNCKFCQNWEISQVRPEQINSFNLPPEKTAQYA
                     MDTGSKSIAYTYTEPMVFYEYMLDCAKAGKARNIRNVVVSAGYIKKEPLIELCKYVDA
                     IKIDFKGFTEKYYQDICHAELKPVMETLIELKKIGIWYEMVYLMLPTLNDDPKDIKSM
                     CDWMVRNLGVDVPIHFTRFYPMYLLKNLPTTPVSSLERAKEIADEAGMKFVYIGNVPG
                     HKAESTYCPKCKNKLIDRTGYRINEMNLKDGKCKFCNEVIPGVWR"
     gene            8902..9165
                     /locus_tag="VGB16_02365"
     CDS             8902..9165
                     /locus_tag="VGB16_02365"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEX9916556.1"
                     /translation="MAISDIISLVAAGLFLLLIFGFCAFVIKRYWIDKKQITSGSQFV
                     SQNVYMQFQNEDKKQAMEQVLYQKEDEREEDDEGDDVSRSDKG"
     gene            9603..12014
                     /locus_tag="VGB16_02370"
     CDS             9603..12014
                     /locus_tag="VGB16_02370"
                     /inference="COORDINATES: protein motif:HMM:NF013713.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEX9916557.1"
                     /translation="MSSNKLNFLLACLLLGFTSMVAQIVFIREMLVVFGGNELCLGVL
                     LFVWLLWVGVGSFVGNRITGDHAVRQAHRPEQSRREGRPYEIHKIYLNQNDSGQTTND
                     EQKATLRVAYPKNIFSLKNLFLWYIVTAILCFLTIFFIRSSKLILGLNTMEIVGFLPM
                     ALSSFILLAPFCFLLGFVFVLNSKVWQLPNISGFLVNRVYLWESIGAGLGGLLATFVF
                     VPKCSNFNIIQIIVLLNLLLGIILWAKYVSKLKLAVVSFLFLVIVFAFVGYQNKLENI
                     SLKLLWRDLPLVKTEDTKYGNIAILKDKEQVSFYENGLLLFSYPAPFSSEEAVHFALS
                     EHSNPQELLLIGGGLGGGLAEALKHDRLKIDYVELDSRLIGLAKEYLPPREAGLFSNP
                     RLDLHLIDGRLFVKNRIGKEKYDVIILNLPDPSTLQLNRFYTVEFFSSIKKILNEDGI
                     FSFRISSSENYISPEQGLYLSSLYGSLSLVFKKVIVFPGDNNIFLASDQESLFYDWSK
                     ITARLKERSIRTEYINQNFLPFRLSQFRIDYLKEAINKSSGRINYDLEPISFFYNMIL
                     WSAQFKSIEKPALLFLYQLNRWWVFSILLGIWLIFLFYSLLRKEKSISLLTLSAIFIT
                     GATSIFLEVVILLSFQVFYGYLYSKVGLILTLFMLGLGAGALYMQNRVKQRDVSLGSL
                     ALLQLYQLSLVFVLVCLITYFSISLIGGYLVELALYLSISFCGMIGGAEFVLANHLYL
                     KERREEKVGMGYSVDLWGSAISSILASVILIPIVGVITVMIIFLLADFVLFIALIFKA
                     RLKIV"
     gene            12191..>12633
                     /locus_tag="VGB16_02375"
     CDS             12191..>12633
                     /locus_tag="VGB16_02375"
                     /inference="COORDINATES: protein motif:HMM:NF012803.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005515 - protein binding [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="PDZ domain-containing protein"
                     /protein_id="HEX9916558.1"
                     /translation="MKDGRKFGAIAVVLTVICLVAIWIIGMGEAVEKKEGEASEYSTI
                     YGYAKLFDMAAWEISHRYVENIDSKEIVYSGIRGMLKNLDAFSSLQDKETHERFMEIT
                     QGKYEGLGMTIGMRGGFIVVSSTFEGSPAYRKGIHPGDKILEIDG"
BASE COUNT         3704 a         2252 c         3042 g         3635 t
ORIGIN      
        1 cttttgtcca tggctaagga tattcgggtc ataatcatca aattggcaga tagattgcac
       61 aatatgagaa ctttggattc acttaacccg gaaaaacaga agaggatagc tcaggaaacc
      121 agagatattt acgctccctt agctcatcgc tttggaatgg ctcggattga atgggagtta
      181 gaggatttaa gtctccgtta cctggagcca gaagtgtatg gggagttact aaagaaagtg
      241 gaggagaaaa gggaagacag ggaagcgtat atccaggaaa taagtcagcc cttacaaaga
      301 gaattaggca aagcaggcat caaagccaaa atcaccggac gagctaaaca tttctacagc
      361 atctaccgca agatgaagaa aagaaacaaa ccattcgaag aaatttatga ccttttcgct
      421 attcgggtga tagtcagcac cgaagcggaa tgctatcatg ttttgggtat cgtgcatacc
      481 ttgtggaaac cggtggagga ccgctttcac gattacatcg ctttgcccaa gagtaatatg
      541 tatcgctccc tgcacaccac gataattggg cctcggggga gaatggtaga aattcaaata
      601 agaacgcacg aaatgcacca tgctgcagaa tacggaatag cggcacattg gctttataaa
      661 gaagggaaga ggaagttgga tgatacagat aagcagatga tttggctaag agaggtcttg
      721 gagtggcaga aggatttaac caatcctaaa gaatttctgg aatatctgaa agttgaccta
      781 tttcacgacg acatcttcgt tttcacgccg aagggagaac tcaggcagtt gccccgaggt
      841 tccacccctc ttgactttgc ctatacagtc cattcggaca tcggcgacca ttgcgttgga
      901 gttaaggtca atggcaggat ggttcccctt tccacagtct tgaatagtgg cgatgaggtg
      961 gatatagtta cctcttctca tcaagccccc agtcaagact ggctgaagat cgtaaagact
     1021 gccaaggcgc gcagcagaat aaaacagtgg ctgaaacaga agggatatga gcagggtatg
     1081 gctttgggaa aggagatctt tgaaaaggag ctgaaaaagc agcatcttcc acttccctct
     1141 gaagaggagc tgatagattt agctatgtcg cttaactatt ctagcttgag tgctatgttt
     1201 tacgctctag gaagtggcgg catctccatg catcagattc tcggaaaatt agttccggag
     1261 aaaaaggaaa taaaggaatc cttagtggaa aagctgattc acaaagccaa aggtgtgggg
     1321 aaagggataa gggttcaggg agaggggaat atgatgttcc gcttcgctca atgctgtcag
     1381 cccctgccgg gtgaaaagat aatggggttc ataactaggg gtagaggagt atcgatccat
     1441 cgagctgact gcaccaatgt tttacaaaca atgggcaatt ccgaccggca ggtggaagtc
     1501 caatgggatg tgggaaagga tgaatcgttt gtggttcgat tggagttggt attagaagac
     1561 aggaaaaata tgctgcggga tataactgaa gcgattacgg atgcggatgc taatgtcaga
     1621 ggcgcggaga ttaaatccgg ggagaccatg gctatggggg atttcctcat cgagattaga
     1681 aacttaaatc atctcaatcg cactatcaag aagattaaag aggtcaaagg ggttgtcgca
     1741 gtcaagagag cagaaggggt agaatttcca catgaataag gaatggaaca aacttgataa
     1801 tggacagata aattggcttg agttttctaa actgaaaaac ataccctttg tttcttttgg
     1861 gctggtggtg aagaattgta aaggcaaatc gtttgccatt gaagtttgct ggaaggataa
     1921 gttcaaaaga tttttgcgca agcaaattgg ctatgctggc gggatagttg ttcccaggca
     1981 ggttcatcaa aataatgttg catccatcac aaaatccgag aagttcaatc ttagaaaaaa
     2041 gcttcaagca gatgctcttc ttaccaatca aaaaggtctc ttcttaacaa ttcaggtggc
     2101 ggattgcatt ccgatatata tagttgatac caaaaagaag gtggtaggat tggtgcatgc
     2161 cggttggagg gggactctga tgcaaatcgc aaaggatgtc gtaggagaag ccagaagaag
     2221 ttttaactgt tcgccggaag atttaaaaat cattttcggt ccggccattg aaaaatgttg
     2281 ctatgaagtc aagccagagg tggctatact ctttcctccc ccatttgtga attacgaagg
     2341 agataagaga cctaagctgg atttagctgg attcaatcga gctcagctaa tagaaagtgg
     2401 tgtgaaaaag aaaaatattt ctttagcagg ggaatgcacc ttttgcggac caaaattctt
     2461 gtgctcctat cgtagaggta ggaataaaaa agagaggatg ttggcattca ttgggatcaa
     2521 ataatgggta gggaacggtt gcaccgttcc cgagaagata aaaggcagta gaagtaatgg
     2581 cgcagccatt acttacccaa tttaaaggga gattgtatga agaaagtttt cttgctttta
     2641 gctgttttct ttttgcttgt ttccccaagc tatgctttca ctggtttagg tttgggggtg
     2701 agggcgggca tcgtcagtaa ttatgacaac cccactttgg atgaactctt cgatgctcct
     2761 tttgtcttga aaggtaagaa gattgaagat atgaacatgt tgggtgccca tcttaaaatc
     2821 gggactttgc cgattattga cttggaaatt tcaggtgagt atttttggaa gactgaagag
     2881 tttagttttg aatttgtgga ctaccagaat ccatctggtg ttgatacagc caatggagag
     2941 tttaaaatag aagactactc tgtgaatgtc actgccaaat atgtttttcc tacatctacg
     3001 atcaaaccat acgttggtgg gggaggggga ttgcatatca taaaatatag taatcctggc
     3061 ccaggccctt tctttgaaaa ccagagcaag cccagttatc acgcctgcgg tggggtgctt
     3121 ttggatgttc cggtcttgcc attagaggtt tttctagagg gaagatatac ctccattcaa
     3181 accgagaaaa aagcgactgg attttttacc tggatggtgg gagtaacgtt aaaccttcct
     3241 tagaagcagg gctgggagcc ctgcctacgg aagttagttt ttgtagctcg aaacgcctcg
     3301 ctcaacctat tagcattaac ggttgggaaa attgagattg cttcgtcgct cgttcctcac
     3361 tcctcgcaat gacagaacaa attaagttcg cgtatgggtg gaacttatct tcacccgtat
     3421 tattctatct atcgtaggcg acccatctgg gtcgcctctt gattttcata tcttttcagc
     3481 cccattctgg tgataaaggt tgacaaggga gaagatttat attattattc cttgcaaatt
     3541 taaccagcta aatactaggg agaaaaaatg aagaagatat ttgcgttttt tgtggttgtt
     3601 ttctttatcc taaccagtgg ttgcctggct caattttcag gacaactctc cacagccaaa
     3661 gttaccaaag agggtggagt tttggggggt ggatacattg gaatttatga agacgccttt
     3721 tccatctttg gacaagtcag gtatggtttc ataaccgacc tggatggcgg attcaaattg
     3781 ggaatattga atttagaccc gggaaatgga ttcggtgaaa gcgaaactgg agtcatcctg
     3841 ggaggagatg ttaagtatca aatcctgaat ttgaaggctg aggaccccct ggatttctcc
     3901 tttgggtttg gaatggaata tctcaatgtt tcagattact ctttgttctc gtttggaggg
     3961 aatttggtcg gctcgtatga tttcgagctg aaaagcaaaa aggttgttac cccctacggc
     4021 agattgaacc tgcgtggaga gagagtggag gtgtcaattc caggatggac aggaagtgat
     4081 gataccgatt ttgatattgc attttcttta ggatgcaatc ttcagatgac atcggaatta
     4141 agtatgatag ctgagttaca gatagatgat aatgttggag tcataggggg aataagctat
     4201 tacatctttt agtctttaag atgacttaaa gctatttgaa aagctctgga cttccccaga
     4261 gcttttttgg tgaaaatatg gaattaacta aggcgatttt tttgggcata gtgcagggct
     4321 taaccgagtt tcttccagtt tccagctctg gtcatttagt tatagtcgag catattttaa
     4381 aagtcaaaat gccaggagtg agctttgaag tgtgtttgca tttggggacc ttcttttcca
     4441 ttgtggtgat ttattgggga aaaatagtaa aaatgattaa ggcaaccata ctaaaagcat
     4501 gctcactttt ttcgggtgga ttcgataaag ccctggagga ggattggaaa ctttcttatt
     4561 cgatagttct gggaactatt cctgctggta tattgggtgt agccctaaaa catcaaatag
     4621 agaatgcttt tacttcaatc agtttagtgg cagttatgct gggaataacc ggtgtcattt
     4681 tgtggttgtc tggatctgcc aaaaccaaaa aggataatgc gggtttattg gatacgattg
     4741 taattggcat agcacaaggt tttgccctct tgccggggat atcgcgctcc gggatgacca
     4801 taagtgctgg gatggcgaga ggaatagata aacccaaagc cgcggaattc tcttttcttt
     4861 tgtccctgcc ggcaatatta ggtgcctctg tacttgagtt ttcaaattta gctgtggaga
     4921 gaaagttttt atttagcaat atagattttt acatagttgg cttcttgact gcttttgttt
     4981 ttggatatat agccataagg tttttgttga gagttatcca aaaagggaaa ttcagatatt
     5041 tcgggtttta ctgcttggca gtggcgattt tagttctgct ttttgtcaga tgacaaaaga
     5101 cataaagaaa atcctaatca ttcgtctcag ctcattggga gacgttgtcc tcaccacccc
     5161 agttataaaa gcgctcaaag ataagctccc gcaggcggaa attcattttt tgaccaaatc
     5221 aaaatacgct tccctcttaa aggataaccc ccatctttct agcataatgg agcttgagga
     5281 taggggagta tctgggctct tgtttactct tcgtaagata agaaagctga atttcgattt
     5341 ggtcatcgac ttacatgcta atctgaggag cttttttata agaagattat ccagagccaa
     5401 gctcaagata agatataata aaagatggat tgcacgcttt ctgatggtga attttaaaaa
     5461 gattaaaatt ccttcacaac acacagttga ttcctatctg agctgtttga aaaggttaga
     5521 tattcatatt tcgaacagga tgcctgagct atacttagat gagaaaagca aggagttcgc
     5581 agagcagtta cttacgggcg tatcaaagga cgacatccta attggaattg ttccgggtgc
     5641 gaaatgggag actaaaaaat ggggagagga aaatttcgct aaagcgattg agattttgaa
     5701 taatagaatc aaagccagtt ttctagtttt tggagctaaa gaggatgagg tcttgataag
     5761 caaattgaaa agtcttgcag gggatattga tttcatggag ataatcggct tgccatttcc
     5821 aaaactctca gccctaatca gtaggtgtgg aataatcttg accaatgact ccggacctat
     5881 gcacatcgca gttgccttga aagtcccggt gttggcgatt tttgggccta cccatcccaa
     5941 gctcggtttt tcccctctgg gagaaaggga tactattttg tgtgctaatg tggaatgcag
     6001 cccctgtagt ttgcacggag agagagaatg ttatcaaaaa accaaaatct gtatggaaaa
     6061 aataacaccc gaaatggtgg cagatgaagt tctggaaatt ctaaaagata gagaaaagat
     6121 atgaaacagt attcctagga ttcttctgga aaatatggac ttgaaggggt aatcgatagg
     6181 gtaacacaac tgcccaatgg gaaatatgaa atacatgact acaaaactac aatccgaggt
     6241 ggaaactgtc cttttgtata actacgccct tttacgaaga ttaaaattag atgagtggga
     6301 agaggagatc agaaagagat taagagagtt tgtgagttta agaaaggata ggcagattta
     6361 ttcctccaag ctcaaggagg aagagtagcc aactagctgg tggtttaagc cgtatttatt
     6421 tgtgtcagga ggctgttttg agtaaatcca gagtgatagt tgttcgccat acgagggttt
     6481 cagataaatc gggcagggtg gattcgaatg tactttccag tatgctctcc gctggtatct
     6541 taaaattgac ccaagaaaaa gacacctccg atggtttgag ccatttcttc aacccaaccg
     6601 accacatagg aatgaaaacc aactgccttt ccggaaaaaa cagctcaact catccggagt
     6661 tggcatacgc catatcttct atgttaggca aatcaggtgt aaagaataag aatatcttta
     6721 tctgggatag gtcaaatcgg gaactgaaag aagctggatt taagataaac acaagtggag
     6781 atggcccgcg ttgtttcgga accgactcct atggggttgg atatgcggaa aaactttata
     6841 cttacaaaaa tatcggtagt cttttttcgc gcatatttat tgatttcact gatactcaga
     6901 ttaatctgcc agtcctaaag gaccatactt tagctggaat aacttgtgga atgaaaaatc
     6961 tctttgggat aattcacaac cccaacaaat atcacagcaa caactgtgac ccttatgtgg
     7021 cggacttatc ctccatgccc ttggttaaga ataaaatcaa acttatcatc tgcgattgca
     7081 taggggttca atacaatgcc ggaccatcct ttcatccgca gtggtgggag aaatatgatg
     7141 gcatcctaat gggcacagac caggtggcag ttgattttgt ggggtacacg attttagacc
     7201 agataagagc aaaaagcggt ttgcctccct tgaagaaaat tggggaggag ccaaaatata
     7261 tcttcactgc ggcggacgtt gagcataatt tgggaaatgt ggatttgaaa aatatagatt
     7321 tggttgaact gaaattagat taaagacgtt tagatgtaag acaaaatgga aggcgacatt
     7381 ccggaatgtc gccttctaag aaggtgataa gagatgtttc gcctgagtcg aagggaattt
     7441 ctaaagagct ctgccttggc cggtgcaacc agtcttctgc cttttggttc aattttgaat
     7501 ctaagccaag aggctttggc ttcagagcgg gatgaaaatc tttcctgggt tgaagcaaaa
     7561 tattacgaca aattacagaa cagggaaatc caatgccaac tctgcccgcg ggaatgcaga
     7621 gtcggagaca gggaaagggg ttattgcgga gtcagagaaa atagagatgg catttattat
     7681 actttagtct attccaaagt ttgttccgcc aacgttgatc ccattgagaa aaagccattc
     7741 tttcattttc ttcccggaac agaggcattt tccattgcca ctgcgggatg caatctcaac
     7801 tgcaaattct gccagaattg ggaaatatcg caagtcaggc ctgaacagat aaatagtttc
     7861 aacttacctc cggaaaaaac ggcgcaatat gccatggaca ctggctccaa gtcgattgcc
     7921 tatacctaca ctgagccgat ggttttctat gagtatatgc tggattgtgc caaagcgggc
     7981 aaagcaagaa atatcagaaa cgtggtggtt tcagctggat atattaaaaa agaaccatta
     8041 attgaactat gtaaatacgt ggatgccata aaaatagatt tcaagggatt cacggaaaaa
     8101 tattatcagg atatctgcca tgcggaattg aaaccggtga tggagacttt aattgagtta
     8161 aagaaaattg gaatctggta tgagatggtt tatctgatgc ttcccacttt gaacgatgac
     8221 cccaaagata taaaatcgat gtgcgattgg atggtaagaa acttaggagt tgacgttcca
     8281 attcatttta cgcgatttta tccaatgtat cttttaaaaa accttcccac gactccggtt
     8341 tcctctttgg agcgggcaaa ggagattgca gatgaggcag gaatgaaatt cgtttatatc
     8401 ggcaacgtgc ccggacataa ggcggaaagc acctattgcc caaagtgtaa gaataaattg
     8461 attgaccgaa cagggtatag gataaacgaa atgaacctga aagatgggaa gtgcaaattc
     8521 tgcaatgaag tgataccggg ggtgtggaga taaggcagtt tagacagttt accccgcacc
     8581 gtacggtgag gggtttaaaa gtttaggagt ttggaattct gtaggggcgg gtcaaaaggc
     8641 ccgcccgctt ggagctgacc tggaagtcta ttgtcatcgc gagcgacccc ggttcatcgg
     8701 gggagcgtgg cgatctcgat ttataaccta tgatgttgta ataagagatt gccacgtcgt
     8761 tcgccagagg cgaaactcct cgcaatgaca gccaaaaacg tttaagtaaa ttaagacacg
     8821 cttttgggtc aaccgcctgc gatggacagt ctaatggcgt caagggcagt ttctcacaca
     8881 atagctaagg aaaaataaaa aatggcaata tctgacataa tttccctggt ggctgctggt
     8941 ttgtttttgc ttttgatttt tgggttttgc gcctttgtca tcaaacgtta ctggatagac
     9001 aaaaagcaga ttacctccgg cagccagttt gttagtcaga acgtttatat gcaatttcag
     9061 aatgaagata aaaagcaggc catggaacag gtcctctacc agaaagagga tgagagggag
     9121 gaagatgacg agggggatga tgtgagtagg tctgataaag ggtaaaaaaa tgattgactt
     9181 gcagtttaaa gctttagata tgatgccctc tgggcaggga acggctgcgc cgttccctga
     9241 agatttgact atagagaaag taatggcgca gccattactt acccggttag gagtttggaa
     9301 ttttgtagga gcgaaccttt aggtccgccc gatcggacag ttcctttcgg tctgtccctg
     9361 cattggtgat aattcgccca atgaatccag ccaaagacgg gataaattag gcaactacaa
     9421 atagataacc ttatccattc cctctcctta aaaaggagag ggttagggag aggttgttaa
     9481 gacgaaggcg acattcgcca atgtcgcctt cggagaatct cgttgtcggg caaggtgcca
     9541 ggctgcgaaa atttaaagcg tcaggagtag cttctgatgc aacatttaac tctcctttca
     9601 caatgtcctc caataaactc aatttccttt tagcttgtct tcttttgggc tttacctcga
     9661 tggttgccca gattgttttc attcgggaga tgctggtggt ttttggcggg aatgagctgt
     9721 gtttgggggt tttgctgttt gtgtggctcc tgtgggtggg agtggggtcg tttgtgggga
     9781 atcgcattac gggcgaccac gcggttcgac aagctcaccg tcctgagcaa agtcgaaggg
     9841 agggtcgccc ctacgaaata cataaaatct atctaaatca gaacgattca gggcaaacta
     9901 cgaatgatga acagaaagcg accctacggg tcgcctaccc taaaaatata ttctccctta
     9961 aaaatctttt cctatggtat atcgtaactg caatcctttg ttttctgacc atattcttca
    10021 tccgctcgtc taagctcatc ttgggtttaa atacaatgga gatagtcggg tttcttccta
    10081 tggcgctttc ctcttttatt ctgctcgctc cattttgctt cctgttgggt tttgtttttg
    10141 tactcaattc aaaagtgtgg caactaccca atatttctgg atttctggtc aatcgagttt
    10201 atttatggga atcgattggt gcaggattag gtggactttt ggcaacgttt gtttttgtgc
    10261 caaaatgttc taatttcaat ataatccaga taatagtttt gttaaatctt ctattgggaa
    10321 taatcctgtg ggcaaaatat gtaagtaaat tgaagttggc agtagtgtct ttcttatttc
    10381 ttgtcatagt atttgcgttt gtgggttatc aaaacaaatt agagaacatc tcccttaaac
    10441 ttttgtggag ggacttacct ttagtcaaaa cagaagatac aaaatatggc aatatcgcca
    10501 tcctaaaaga taaagagcag gtaagtttct atgaaaacgg gcttttgctt ttttcctatc
    10561 cggcaccttt ttcgtctgaa gaagcagtcc attttgcttt gagcgagcat tcaaatccac
    10621 aagagcttct tttaattgga ggtggattgg gaggagggtt ggctgaagcc ttaaaacatg
    10681 atagactcaa aatagactac gtggaattag atagcagatt aataggatta gccaaagagt
    10741 atcttcctcc aagagaagca ggcttgtttt ccaatccgag attggatctg catctgatag
    10801 atggccggct attcgtcaag aacagaatcg gaaaagaaaa atacgatgtc ataatcttaa
    10861 atctacctga cccttcaacc ttacagctta accgcttcta tacagtagag ttctttagtt
    10921 caattaagaa gattctgaat gaggatggaa tattctcttt cagaatcagt tcatctgaaa
    10981 attacatcag tcctgagcaa ggactttacc tttcgagcct atacggaagc ttatctttgg
    11041 tttttaagaa agtaatcgtt tttccgggag acaacaatat cttcttagca tcagaccaag
    11101 aaagtctttt ttatgattgg tcaaagataa cagctcgctt aaaagaacgg tcaatccgaa
    11161 ctgaatatat taatcaaaac tttttaccct tcaggttatc acaattcaga atcgattact
    11221 taaaagaggc aataaacaag tcttctggca ggataaatta cgacttagag ccaatctcgt
    11281 ttttctataa catgatcctg tggagcgccc aattcaaatc aattgagaag ccagctctct
    11341 tgttcttgta tcagcttaat cgttggtggg tattctctat tcttttagga atctggttaa
    11401 tttttctttt ctattccctt ttaagaaaag aaaagtcaat ttcgcttctg actctgtccg
    11461 ccattttcat aaccggtgcc accagcatat ttttagaagt agtcatactc ttatcctttc
    11521 aagtttttta tgggtatctt tattccaagg tgggattgat tttgacttta tttatgttag
    11581 gtctaggcgc tggcgcttta tatatgcaaa acagagttaa acaaagagat gtatctcttg
    11641 gcagtttagc tttattgcaa ctttatcagc tttctttggt ttttgtgctc gtgtgtttga
    11701 ttacctactt ttcaatcagt ttaattggcg ggtatttggt tgagctagct ttatatttat
    11761 ccatcagttt ttgtgggatg attggtgggg cggagttcgt tttagctaac catctctacc
    11821 tgaaagaaag aagggaagag aaagttggga tggggtattc agttgacttg tgggggagcg
    11881 caataagttc cattttagcc agtgtgattt tgattccaat tgtgggtgtg ataacagtta
    11941 tgattatttt tctgttagcg gattttgttc tcttcatagc acttatcttc aaagccagac
    12001 ttaagattgt ttaactacaa taacagatga tatatcaacc aattatggcc attacatcaa
    12061 ctgttacttt aaatacggaa aatcgatttc aaatttccac aaaaaaactt gaaactgttt
    12121 tgttgacaaa gagcatattt tgattaaatt gaacggcgca ggaggggtgg gggcaaagtc
    12181 gggagggatt atgaaggatg gaagaaagtt tggagcgatt gcagtcgtct taaccgtaat
    12241 ctgcttagtg gcgatttgga taattggaat gggtgaggcg gttgaaaaaa aggagggaga
    12301 ggctagtgag tattcgacga tctatggtta tgccaagcta ttcgacatgg ccgcttggga
    12361 gataagccat agatatgtgg aaaatattga ttcaaaggag atagtctact ccggcattag
    12421 agggatgtta aagaatctgg atgccttttc gtccttacag gataaggaaa ctcatgaaag
    12481 attcatggag atcacccagg ggaaatacga gggtttggga atgactatcg gaatgagagg
    12541 tggtttcatt gtggtcagtt ccacttttga agggtctccg gcttatcgca aagggatcca
    12601 tccgggagat aaaattctag aaatagatgg caa
//
LOCUS       DASWLN010000070         2385 bp    DNA     linear   ENV 20-FEB-2024
DEFINITION  MAG TPA_asm: Candidatus Zixiibacteriota bacterium isolate
            SMAG_U2949 k141_68463, whole genome shotgun sequence.
ACCESSION   DASWLN010000070 DASWLN010000000
VERSION     DASWLN010000070.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37960594
            Sequence Read Archive: SRR10376091
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Candidatus Zixiibacteriota bacterium (soil metagenome)
  ORGANISM  Candidatus Zixiibacteriota bacterium
            Bacteria.
REFERENCE   1  (bases 1 to 2385)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 2385)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (26-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/12/2024 08:28:26
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 1,440
            CDSs (total)                      :: 1,416
            Genes (coding)                    :: 1,412
            CDSs (with protein)               :: 1,412
            Genes (RNA)                       :: 24
            rRNAs                             :: 1 (16S)
            partial rRNAs                     :: 1 (16S)
            tRNAs                             :: 21
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 4
            CDSs (without protein)            :: 4
            Pseudo Genes (ambiguous residues) :: 0 of 4
            Pseudo Genes (frameshifted)       :: 0 of 4
            Pseudo Genes (incomplete)         :: 3 of 4
            Pseudo Genes (internal stop)      :: 1 of 4
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2385
                     /organism="Candidatus Zixiibacteriota bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_68463"
                     /isolate="SMAG_U2949"
                     /isolation_source="soil"
                     /db_xref="taxon:2053527"
                     /environmental_sample
                     /country="USA: Oregon"
                     /lat_lon="42.99 N 123.63 W"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            complement(327..728)
                     /gene="mce"
                     /locus_tag="VGB16_02380"
     CDS             complement(327..728)
                     /gene="mce"
                     /locus_tag="VGB16_02380"
                     /EC_number="5.1.99.1"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003870713.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="methylmalonyl-CoA epimerase"
                     /protein_id="HEX9916559.1"
                     /translation="MKIKAIDHIGVAVKDIRQASRFYGLLGLEVSKEYDLPSQKIRIA
                     YIDTPATKIELIQPISEDSIISKFIEKKGEGIHHLCFVVDDIEKSLAELKSKGVQLID
                     EKPRMNPHNEKIAFINPKSVNGVLIELKEKM"
     gene            complement(730..2280)
                     /locus_tag="VGB16_02385"
     CDS             complement(730..2280)
                     /locus_tag="VGB16_02385"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_006309686.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="methylmalonyl-CoA mutase family protein"
                     /protein_id="HEX9916560.1"
                     /translation="MKELYTPKDLEGFDYQHDLGFPGEYPFTRGIQKDMYLGKLWTMR
                     QYAGFGTAEETNKRYKYLLKQGQTGLSVAFDLPTQIGYDSDHQLSKGEVGRTGVAIDS
                     LKDMETLFNGIPLDKVSTSMTINSTAAVLLAMYVVLAKKSNVSLERLSGTIQNDILKE
                     YIARGTYIFPPKESMRLTIDIFKFCKDNLPKFNPISISGYHIREAGATAVQEIAFTLA
                     NGIAYVKAALEAGMKLEEFADRLSFFFSSHNNFLEEVGKFRAARRLWARMMREKFNAK
                     NNNSCRLRFHTQTAGSTLTAQQPDNNIVRVAFQALAAVLGGTQSLHTNSKDEALGLPS
                     EGAVQTALRTQQIIAYESGVAQTIDPLAGSYAIETLTNRIEKEAQEQIDYIERNGGAL
                     WAVESGYYKREIQKSAYQYQKQIEKEQKIVVGVNKFSIDEKRKRDIFKVDSKIENQQV
                     KRLKLLRSKRNNQEVKASLDRLRRKSQTDENLLPYMISCVENYATLGEICGVLREVWG
                     EYRETVIV"
BASE COUNT          578 a          564 c          434 g          809 t
ORIGIN      
        1 gttttggttc ttcttctgct tccggtgcat tttccggcac cttattttcg gcgccacagt
       61 tttgacattt tgcgacttca cccactttca gaaatttcgt gatgatgtac tcgccacact
      121 gccgacactt gaattttaat gccatttccg cctccttcga ttttgcgggt caagggtccg
      181 caaggatgct tgggaaccca tttcgtcgag aaatccttta gacatgctta acctactaaa
      241 ccgctatcta ttcaaacacc atcaacacct gatccttgtc cactgtctgt tgttctttca
      301 ctaagggcat acctaaaggt ctgtccctac atcttctctt tcaactcaat taaaaccccg
      361 tttacacttt tgggatttat aaatgcgatt ttctcattgt gcggattcat tcttggtttt
      421 tcatcgatta gttgcacacc cttggatttc aattcggcca atgatttctc gatgtcatcc
      481 accacaaaac aaagatgatg tattccctct cctttttttt cgataaactt ggagataatg
      541 ctatcctctg aaatcggttg tatgagctca attttggttg caggagtatc aatataagct
      601 attcttatct tttgagaagg taaatcgtat tccttggaaa cctcaagtcc caaaagtccg
      661 taaaacctac ttgcctgcct tatatctttg accgctactc cgatatgatc tatcgctttt
      721 attttcatct tatactataa ctgtttccct gtattccccc cagacttccc gcaagacgcc
      781 acatatctca cccaaagttg cgtagttttc gacgcagctt atcatatagg ggagcaaatt
      841 ctcgtctgtt tgtgattttc gcctcaacct atccaaggag gctttaactt cttgattgtt
      901 tctcttcgaa cgcagaagct tcaatctttt gacttgctga ttttcaattt tcgaatccac
      961 cttaaagata tcccttttcc ttttttcatc aatcgagaac ttattaaccc ccactactat
     1021 tttttgctct ttttcgattt gtttttgata ttggtaagca cttttctgaa tttccctttt
     1081 ataatagccg gactcaaccg cccacaaagc tccaccattt ctttcaatat agtcgatctg
     1141 ttcttgtgcc tctttttcga ttctgttggt caaggtctca atagcataag agccggccag
     1201 agggtcgatg gtttgagcta cgcctgattc gtaggcgatt atctgctgag tacgtaaagc
     1261 ggtttgcact gccccttcgg agggaagacc caaagcttca tcctttgagt tagtgtgaag
     1321 ggattgagtt cctcctaaaa ctgcagctaa ggcctgaaat gccactcgca caatattgtt
     1381 gtctggttgc tgggcagtta gagtgcttcc tgcggtttgg gtgtgaaaac gcagacggca
     1441 agagttatta tttttggcat tgaatttttc tcgcatcatc ctcgcccata atcgtcgtgc
     1501 cgccctgaac ttgccaacct cttctaggaa attgttatgc gaactgaaaa agaacgacaa
     1561 tctgtcagca aattcctcta gtttcattcc tgcttccaaa gccgccttga cgtaagctat
     1621 cccattggcc aaggtgaagg ctatttcttg aactgcagtt gccccggctt ctctgatatg
     1681 atacccgctg atactgatgg gattgaattt gggaaggtta tccttacaga atttaaagat
     1741 atcaattgta aggcgcatag attccttggg tggaaaaatg taagttcccc gtgcgatata
     1801 ttcttttaaa atatcattct gaattgtgcc agatagtctt tctaatgata cgttcgattt
     1861 cttagctaaa accacataca tcgctaaaag gactgctgcc gtggagttga tagtcatcga
     1921 agtgctaact ttatccaaag gtatcccgtt aaagagcgtt tccatatctt ttaaactatc
     1981 gattgccact ccagttctgc caacttcacc tttggacagt tgatggtctg aatcatatcc
     2041 aatctgggta ggaaggtcaa aggcaactga taagccggtt tgaccttgtt tcaatagata
     2101 cttgtatctt ttattggtct cctctgcagt tccaaatcca gcatactgcc gcatagtcca
     2161 gagttttccc agatacatat ccttttgaat accgcgagta aaaggatact ctccgggaaa
     2221 tcctaagtcg tgctggtaat caaaaccctc taaatctttc ggagtgtaaa gttctttcat
     2281 cttactcaaa aacgattagg gtttcatctt tctccactgt ttgtccgggc ttgactttca
     2341 cttcttttat cttggcatca aattttgctt ttaactcatt ttcca
//
LOCUS       DASWLN010000071         1734 bp    DNA     linear   ENV 20-FEB-2024
DEFINITION  MAG TPA_asm: Candidatus Zixiibacteriota bacterium isolate
            SMAG_U2949 k141_158647, whole genome shotgun sequence.
ACCESSION   DASWLN010000071 DASWLN010000000
VERSION     DASWLN010000071.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37960594
            Sequence Read Archive: SRR10376091
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Candidatus Zixiibacteriota bacterium (soil metagenome)
  ORGANISM  Candidatus Zixiibacteriota bacterium
            Bacteria.
REFERENCE   1  (bases 1 to 1734)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 1734)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (26-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/12/2024 08:28:26
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 1,440
            CDSs (total)                      :: 1,416
            Genes (coding)                    :: 1,412
            CDSs (with protein)               :: 1,412
            Genes (RNA)                       :: 24
            rRNAs                             :: 1 (16S)
            partial rRNAs                     :: 1 (16S)
            tRNAs                             :: 21
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 4
            CDSs (without protein)            :: 4
            Pseudo Genes (ambiguous residues) :: 0 of 4
            Pseudo Genes (frameshifted)       :: 0 of 4
            Pseudo Genes (incomplete)         :: 3 of 4
            Pseudo Genes (internal stop)      :: 1 of 4
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..1734
                     /organism="Candidatus Zixiibacteriota bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_158647"
                     /isolate="SMAG_U2949"
                     /isolation_source="soil"
                     /db_xref="taxon:2053527"
                     /environmental_sample
                     /country="USA: Oregon"
                     /lat_lon="42.99 N 123.63 W"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            <1..404
                     /locus_tag="VGB16_02390"
     CDS             <1..404
                     /locus_tag="VGB16_02390"
                     /inference="COORDINATES: protein motif:HMM:TIGR00046.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=3
                     /transl_table=11
                     /product="RsmE family RNA methyltransferase"
                     /protein_id="HEX9916561.1"
                     /translation="VQFRNQVQEKNKTERWGRVATSAMKQSLRCFLPKVSQVTKLEDL
                     LKNIKRYDLALMASLESRSRKLEDLSELKNRPKNVLLLVGPEAGFSSDELGEALGRGV
                     FPVTLGARRLRSETAGVLFSSLVLYELKELA"
     gene            510..716
                     /gene="rpsU"
                     /locus_tag="VGB16_02395"
     CDS             510..716
                     /gene="rpsU"
                     /locus_tag="VGB16_02395"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_005033802.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0000314 - organellar small ribosomal
                     subunit [Evidence IEA];
                     GO_component: GO:0022627 - cytosolic small ribosomal
                     subunit [Evidence IEA];
                     GO_function: GO:0003735 - structural constituent of
                     ribosome [Evidence IEA];
                     GO_process: GO:0006412 - translation [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="30S ribosomal protein S21"
                     /protein_id="HEX9916562.1"
                     /translation="MTGVKVRDNESFEKALRRFNKTCERSGILADIKKHQHFEKPSER
                     RKRKRNAAKRKSRQAEKPQSGGIK"
     gene            717..1190
                     /locus_tag="VGB16_02400"
     CDS             717..1190
                     /locus_tag="VGB16_02400"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_013537282.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="GatB/YqeY domain-containing protein"
                     /protein_id="HEX9916563.1"
                     /translation="MGLKEKIDKDMVEAQKAGDKLKLSVTRMLKSEIRYKEIEKKDQL
                     DEAGLMDVLSSAAKRRRESIEGFSKGNRPDLVEKEKQELEIIQQYLPQQMTSVEVSQL
                     VDEIISEVGAVGASDKGKVMKALMPRLKGRFDGKEASLLVASRLDQKPSQGGELP"
     gene            1207..>1734
                     /locus_tag="VGB16_02405"
     CDS             1207..>1734
                     /locus_tag="VGB16_02405"
                     /inference="COORDINATES: protein motif:HMM:NF014704.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="CvpA family protein"
                     /protein_id="HEX9916564.1"
                     /translation="MNWIDLVLAVLMVLAIVICSRRGLFSSIMTLIGLYVGIILGIQY
                     IDTSTQRILSHFRGSPIIIALFSFTMTFLLVYLAAKILGSMFYKFASLHSLGNLDKLG
                     GGILGVFVGWIFLGILLFVLIFLPLPDRLVTKINESTLGLAMRGTVAFIYEEGSSLFH
                     PNSPRLVTKVKSALSY"
BASE COUNT          518 a          308 c          466 g          442 t
ORIGIN      
        1 aagtccagtt cagaaaccag gtgcaggaga agaacaaaac agaaagatgg gggcgtgtgg
       61 caacaagtgc gatgaagcaa tccttgcgct gtttcttgcc caaagttagt caggtcacaa
      121 aactggagga cttactcaaa aatataaaaa gatacgattt ggctctgatg gcaagtctgg
      181 agagcagaag cagaaaactt gaggatttat ccgagctgaa gaatagaccc aagaatgttc
      241 tacttttggt tggtccggaa gccggttttt cttcagatga gcttggggaa gctctgggaa
      301 gaggtgtgtt tcctgtaacc ctcggagcaa gaagactgag aagcgagacc gccggggttt
      361 tattttcgtc tctggtattg tatgaactta aagagttagc ttgacaagag aaactctttt
      421 agttatttta acatgttggg taattctttt actaaaacag atatcattta agaataactt
      481 ctaacctttg aaagggggtt ggagatgcga tgactggtgt taaggtgcga gacaacgaat
      541 cctttgagaa ggcattacgg cgattcaaca agacttgcga gaggtcgggc atattggctg
      601 atataaagaa gcatcaacat tttgagaagc cttcggagcg cagaaaaagg aagagaaatg
      661 cagccaagcg gaaaagccgc caagcggaaa agccgcagag tggaggaata aagtagatgg
      721 gcttgaagga gaaaatcgat aaggacatgg tggaggctca gaaggcgggt gataagctca
      781 agctcagcgt aacccggatg cttaagtcag agataaggta taaagagata gaaaagaagg
      841 accagctgga cgaagccggg ctgatggatg tcttgagttc agctgccaaa aggcgtcggg
      901 aatcaattga ggggtttagt aaaggaaaca ggcctgactt ggttgaaaag gaaaagcagg
      961 agttggagat aatacaacag tacctgccac aacagatgac cagcgtggaa gtcagtcagc
     1021 ttgtagatga aatcatttcc gaagtgggag ctgtgggagc gtctgacaag ggaaaggtta
     1081 tgaaagctct catgccaaga cttaagggaa ggtttgacgg taaagaagca agccttttgg
     1141 ttgcctcgag attagatcaa aaaccatctc aaggaggaga actaccttag aaaaagaggt
     1201 gtttctatga actggatcga tttggttttg gctgttctca tggtactggc aatagtaatc
     1261 tgctctagaa gggggctgtt tagtagtata atgactttga tcggacttta tgtcggtatt
     1321 atactgggaa tacaatatat cgatacaagc actcaaagga tactctccca tttcagaggt
     1381 tcgcccataa taatcgcgct gttcagcttc actatgacct tccttttggt atatctagcc
     1441 gccaagattt tgggttctat gttttacaaa ttcgcctcgc tccattcgct gggcaacctc
     1501 gacaagctgg gtggagggat tctgggggtt ttcgttggct ggatattttt gggcatactc
     1561 cttttcgttt tgatattctt acctctgccg gatagattgg taacaaaaat aaatgaatca
     1621 actctgggtc tggctatgag agggacagtc gccttcattt acgaggaagg gtcgagtctg
     1681 tttcatccca atagcccacg tcttgtgaca aaagtcaaat ctgctctctc atat
//
LOCUS       DASWLN010000072         2274 bp    DNA     linear   ENV 20-FEB-2024
DEFINITION  MAG TPA_asm: Candidatus Zixiibacteriota bacterium isolate
            SMAG_U2949 k141_323864, whole genome shotgun sequence.
ACCESSION   DASWLN010000072 DASWLN010000000
VERSION     DASWLN010000072.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37960594
            Sequence Read Archive: SRR10376091
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Candidatus Zixiibacteriota bacterium (soil metagenome)
  ORGANISM  Candidatus Zixiibacteriota bacterium
            Bacteria.
REFERENCE   1  (bases 1 to 2274)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 2274)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (26-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/12/2024 08:28:26
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 1,440
            CDSs (total)                      :: 1,416
            Genes (coding)                    :: 1,412
            CDSs (with protein)               :: 1,412
            Genes (RNA)                       :: 24
            rRNAs                             :: 1 (16S)
            partial rRNAs                     :: 1 (16S)
            tRNAs                             :: 21
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 4
            CDSs (without protein)            :: 4
            Pseudo Genes (ambiguous residues) :: 0 of 4
            Pseudo Genes (frameshifted)       :: 0 of 4
            Pseudo Genes (incomplete)         :: 3 of 4
            Pseudo Genes (internal stop)      :: 1 of 4
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2274
                     /organism="Candidatus Zixiibacteriota bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_323864"
                     /isolate="SMAG_U2949"
                     /isolation_source="soil"
                     /db_xref="taxon:2053527"
                     /environmental_sample
                     /country="USA: Oregon"
                     /lat_lon="42.99 N 123.63 W"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            complement(<1..835)
                     /locus_tag="VGB16_02410"
     CDS             complement(<1..835)
                     /locus_tag="VGB16_02410"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_014400040.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ABC transporter substrate-binding protein"
                     /protein_id="HEX9916565.1"
                     /translation="MKRDCKIYWLLSLGLIFWMLFSCAKKEDVVILGEFGSLTGVTAT
                     FGKSTQRGIQMAVEEINKSGGINGKLVRVIVEDDQSKPEEAATVVKKLVNQDKVLIVL
                     GEVASSRTLAGAPSCQEAGVPLITPASTNPKVTEVGDYVFRVCFIDPFQGEVMAKFAR
                     NTLKVSKAAILKDIKNDYSVGLAQFFTETFKSLGGIIVAKESYSEGDIEFRAQLTAIK
                     ARNPEVIFIPGYYTEVGLIARQARDLGITVPLIGGDGWDSPRLVEIGGKALENTYYSN
                     HY"
     gene            1039..1812
                     /gene="mtgA"
                     /locus_tag="VGB16_02415"
     CDS             1039..1812
                     /gene="mtgA"
                     /locus_tag="VGB16_02415"
                     /EC_number="2.4.1.129"
                     /inference="COORDINATES: protein motif:HMM:TIGR02070.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0009274 - peptidoglycan-based cell wall
                     [Evidence IEA];
                     GO_component: GO:0016020 - membrane [Evidence IEA];
                     GO_process: GO:0009252 - peptidoglycan biosynthetic
                     process [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="monofunctional biosynthetic peptidoglycan
                     transglycosylase"
                     /protein_id="HEX9916566.1"
                     /translation="MRVLKAGQFKWQYILLILLLVVILVFFLEYLTLPDVSYLQKENP
                     KTTSLIQIRNKEYKKRGKKPVEYRIWVPYSQISPYLKKAILVGEDINFYRHSGIDLEE
                     MKESLKINWKKKGFSRGASTITQQLAKNLYLSPSKNPFRKLREILIAFQLERSLSKRR
                     IFELYMNLVEWGDGIYGCEAASRFYFGKSSSEFSPDEAIRMATSLPFPRKYPPTSEKR
                     RFEKRRKLILSRMIRYGFVTQGEYDSSFDPEPHESKDSF"
     gene            1918..2145
                     /gene="ssb"
                     /locus_tag="VGB16_02420"
     CDS             1918..2145
                     /gene="ssb"
                     /locus_tag="VGB16_02420"
                     /inference="COORDINATES: protein motif:HMM:TIGR00621.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003697 - single-stranded DNA binding
                     [Evidence IEA];
                     GO_process: GO:0006260 - DNA replication [Evidence IEA];
                     GO_process: GO:0006281 - DNA repair [Evidence IEA];
                     GO_process: GO:0006310 - DNA recombination [Evidence IEA];
                     GO_process: GO:0006351 - DNA-templated transcription
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="single-stranded DNA-binding protein"
                     /protein_id="HEX9916567.1"
                     /translation="MASLNKLFLIGKLGKHTARRYTPNGQVVISVYLGTTFRWKNKSI
                     QFQEITDWHNLVIWEKQAEIGKDIPKRTRSL"
BASE COUNT          688 a          481 c          448 g          657 t
ORIGIN      
        1 tataatgatt actgtaataa gtattctcca aagcctttcc tcctatttcc accagacgcg
       61 gggaatccca tccatctcca ccaattaagg gaaccgtgat ccccaaatct cttgcctgac
      121 gcgcaattaa acccacttcg gtataataac cgggaatgaa gataacctcc ggatttctgg
      181 ctttgatggc agtcaattgt gcccggaact ctatatctcc ttcggaataa gattcctttg
      241 ctacgattat cccacccaag cttttaaagg tttcggtaaa gaattgtgcc aggcccacgc
      301 tgtaatcatt ttttatgtct ttgaggattg cggctttact cactttcagg gtatttcgtg
      361 caaatttagc catcacctca ccctggaaag ggtcgatgaa acagactcta aaaacgtagt
      421 cacccacctc agtaaccttg ggattggtag aagcaggggt aatcaaaggc acgccagctt
      481 cctgacaact tggagcgccg gctaaggttc tggaggaagc cacctcaccc aagacgataa
      541 ggactttatc ctggttaact aacttcttaa ccacagtagc cgcctcttcc ggcttggatt
      601 ggtcatcttc cactatgacc ctgaccaact ttccatttat tcctccagat ttgtttatct
      661 cctccacagc catctggatt cccctctggg ttgacttgcc gaaagtggca gttactccgg
      721 tcaaggagcc gaattcacct aagatgacaa catcctcttt tttagcacaa gagaatagca
      781 tccaaaagat taaacccaat gaaagcaacc aataaatctt gcagtccctt ttcatcaact
      841 ttctcctttg ctcattctgt gatttgttta acttaaatat atccgaaatc gataattgtc
      901 aagccatttt tagactgtgg ttttcgtact aactcaacag ttctttgtat agttttcgat
      961 tcgaaatgac aaaaatttca ttgcgatttt cgatttcata acttaaaata gagcaaggat
     1021 attgttggag ctgaaattat gagagttctg aaagcaggac aattcaaatg gcaatacata
     1081 ttgttaatcc tgcttttggt tgttatcctt gtctttttct tagaatatct gaccctgccg
     1141 gacgtaagtt atctccaaaa ggaaaatcct aaaaccactt cccttattca gataagaaac
     1201 aaagaatata aaaagagagg gaaaaaacct gtagaatatc gcatttgggt gccatatagc
     1261 cagatctcgc catacttgaa gaaggctatc ttagtaggag aggatataaa tttctataga
     1321 catagcggaa tagatttaga ggagatgaag gagtctttaa agatcaactg gaagaagaag
     1381 ggattttccc gaggcgcctc caccattacc caacaattag ccaaaaacct ttatctctcc
     1441 ccttccaaaa atccatttag aaaattaagg gaaattttaa ttgctttcca attggaaagg
     1501 agcttgagta aaagaaggat tttcgaattg tatatgaatc tggttgaatg gggggatgga
     1561 atttatggtt gcgaggcggc ttccagattc tattttggaa aatcttcttc cgagttttca
     1621 ccggatgagg ctattcgtat ggcgacctca ttgccctttc cccgaaaata ccctcctact
     1681 tcagaaaaga ggagatttga gaaaaggaga aaactgattt taagtcggat gataagatat
     1741 ggatttgtga ctcaagggga atacgactcc tcctttgacc cagagcccca tgaatcaaaa
     1801 gattcattct agatctgaat cttctatgtt tgattttctc aagaggccta actgaaaacg
     1861 gttgacagtc gtcaaaataa atactatcat ataaaaaata ttgaatccgg atgaaaaata
     1921 gcaagtttaa acaagttatt tttaatcgga aaactgggta aacatactgc aagaagatac
     1981 accccgaatg gtcaagtagt gattagtgtt tacttaggta ctaccttccg ttggaaaaat
     2041 aaatctatcc agtttcagga gataaccgac tggcataact tagtgatttg ggaaaagcaa
     2101 gctgaaattg gcaaggatat cccgaaaagg accagatctc tgtgagagga aggtccgatc
     2161 cgttactatg atgaacgtaa tgtgaatatg ccacggtttt aaggttcgac tttgctccct
     2221 tcgactttgc tcagggacag tgagcggttc gacttcgctc accgtcctga actt
//
LOCUS       DASWLN010000073         2520 bp    DNA     linear   ENV 20-FEB-2024
DEFINITION  MAG TPA_asm: Candidatus Zixiibacteriota bacterium isolate
            SMAG_U2949 k141_324804, whole genome shotgun sequence.
ACCESSION   DASWLN010000073 DASWLN010000000
VERSION     DASWLN010000073.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37960594
            Sequence Read Archive: SRR10376091
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Candidatus Zixiibacteriota bacterium (soil metagenome)
  ORGANISM  Candidatus Zixiibacteriota bacterium
            Bacteria.
REFERENCE   1  (bases 1 to 2520)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 2520)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (26-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/12/2024 08:28:26
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 1,440
            CDSs (total)                      :: 1,416
            Genes (coding)                    :: 1,412
            CDSs (with protein)               :: 1,412
            Genes (RNA)                       :: 24
            rRNAs                             :: 1 (16S)
            partial rRNAs                     :: 1 (16S)
            tRNAs                             :: 21
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 4
            CDSs (without protein)            :: 4
            Pseudo Genes (ambiguous residues) :: 0 of 4
            Pseudo Genes (frameshifted)       :: 0 of 4
            Pseudo Genes (incomplete)         :: 3 of 4
            Pseudo Genes (internal stop)      :: 1 of 4
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2520
                     /organism="Candidatus Zixiibacteriota bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_324804"
                     /isolate="SMAG_U2949"
                     /isolation_source="soil"
                     /db_xref="taxon:2053527"
                     /environmental_sample
                     /country="USA: Oregon"
                     /lat_lon="42.99 N 123.63 W"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            75..572
                     /gene="fliO"
                     /locus_tag="VGB16_02425"
     CDS             75..572
                     /gene="fliO"
                     /locus_tag="VGB16_02425"
                     /inference="COORDINATES: protein motif:HMM:NF016250.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_process: GO:0044781 - bacterial-type flagellum
                     organization [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="flagellar biosynthetic protein FliO"
                     /protein_id="HEX9916568.1"
                     /translation="MRKTLVAITIPLCLFMSNGAWGDQMDFMNKGANSGPSFASLLVR
                     LVFALVLIVGLIYASMYALKRFSYSGKKKLGGSNIEVLQRSYLAPKKGIYIVKVQSKI
                     LVLGVTDNNINLLTELPELADEGGDPQNPFSNPGDKSKNFSDLMQEMKGKFNSLWQKR
                     GKDAV"
     gene            562..1329
                     /gene="fliP"
                     /locus_tag="VGB16_02430"
     CDS             562..1329
                     /gene="fliP"
                     /locus_tag="VGB16_02430"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_018704286.1"
                     /note="The bacterial flagellar biogenesis protein FliP
                     forms a type III secretion system (T3SS)-type pore
                     required for flagellar assembly; Derived by automated
                     computational analysis using gene prediction method:
                     Protein Homology.
                     GO_process: GO:0001539 - cilium or flagellum-dependent
                     cell motility [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="flagellar type III secretion system pore protein
                     FliP"
                     /protein_id="HEX9916569.1"
                     /translation="MQFKSTSKIILIAIVLFSLVLFMSPQRSFAQGLPKISLGIEKSS
                     NRGDLSVTLQIVLLITILSLAPAIVMMVTSFTRIVVVFSFLRHAIGTQQMPPNQLLIA
                     MALILTFFVMAPVINQINTDAVQPYLKGTISQDDAYKLGVKPLREFMLRQTREKDLTL
                     FVDLAKIPTPNTSDDIPTYVLIPGFMISELRIAFQISFVLFIPFLIIDMVVASVLMSM
                     GMLMLPPVMISMPFKILLFVLVDGWYLIVKSLVTSFH"
     gene            1353..1622
                     /gene="fliQ"
                     /locus_tag="VGB16_02435"
     CDS             1353..1622
                     /gene="fliQ"
                     /locus_tag="VGB16_02435"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_020267889.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0009288 - bacterial-type flagellum
                     [Evidence IEA];
                     GO_process: GO:0001539 - cilium or flagellum-dependent
                     cell motility [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="flagellar biosynthesis protein FliQ"
                     /protein_id="HEX9916570.1"
                     /translation="MTTEFVIGIGREAILITLMVSAPMLVFGLIVGLAVSILQAVTQV
                     HEMTLIFIPKIIAVALALLIFLPWILNKVVDFTAKLINSIPNIAM"
     gene            1844..2470
                     /locus_tag="VGB16_02440"
     CDS             1844..2470
                     /locus_tag="VGB16_02440"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEX9916571.1"
                     /translation="MAKKTLVIVLVLFFVLSGVSWGIERVERNRRGTFLFVDGGWTTL
                     DEHSSRDGFSLGFGIGGNFSGHHIGRFSFRYCQYGYKFSQTGSKWTGKIFDYGLSLKT
                     TIIDVHRHPIANPYFGFGLGLMYAISRYEYSDIVFYPYQVEQDSRFFMSFNFFTGCDF
                     ALTEKSWLLLEGGFLMDIQEPTLFRDEKVSIRSRMENYRVVIGFGHKL"
BASE COUNT          683 a          460 c          568 g          809 t
ORIGIN      
        1 caagatgggt ttaccgatta ctcaacttat ctccaagagc aatctgatta agaatctata
       61 gtctgttcaa tcaaatgaga aaaaccttag tcgctattac tatacccctt tgtctattta
      121 tgtccaatgg ggcttggggt gaccagatgg atttcatgaa caaaggggca aactctggtc
      181 ccagctttgc cagtctctta gtgagattgg tttttgcctt ggtcttgatt gtgggactaa
      241 tttacgcttc aatgtatgcc ttgaaaagat tttcctatag cggtaagaaa aaattaggtg
      301 ggagtaatat agaagttctg caaagaagtt accttgctcc taaaaaggga atttatatag
      361 ttaaagtcca atccaaaatc ttggttttag gagttactga taacaacatc aatcttttga
      421 ctgaattgcc agaattagcg gatgaaggag gagaccctca aaatcctttt tctaatcccg
      481 gggataaaag caaaaacttc tccgatttaa tgcaagagat gaagggcaaa ttcaattccc
      541 tatggcagaa gagaggaaaa gatgcagttt aaatcaacca gcaaaatcat actaatcgcc
      601 attgtcttgt tctcactggt tttgtttatg tccccacaaa ggagctttgc tcaaggatta
      661 cccaagattt ctttgggaat agagaaatca tccaataggg gagacctttc agttactttg
      721 cagatagttt tgttgattac catattgagc ttggccccag ccatagtgat gatggttacc
      781 tccttcaccc gtattgtggt agtattctca tttttgaggc acgccatagg aacacagcag
      841 atgccgccaa atcaactcct aatagctatg gctttaatct tgaccttttt cgtaatggct
      901 ccggtgataa atcagataaa cacagacgca gtccaacctt atctgaaggg aacgataagt
      961 caagatgatg cttataaact gggagtaaaa cccttaaggg agtttatgct cagacaaacg
     1021 cgggagaagg atttgactct gtttgttgat ttggctaaga ttccaacccc taataccagc
     1081 gacgatatcc caacttatgt tctgattccg ggatttatga taagcgaact gcggatcgct
     1141 tttcagataa gctttgttct ttttataccg tttctgataa tagatatggt ggtggcatcg
     1201 gttctgatgt ctatgggaat gctgatgctt ccgccagtta tgatttctat gccgtttaag
     1261 atactacttt tcgttttggt ggacggctgg tacttaatag tgaaatcctt agttaccagc
     1321 tttcactgac aaaaaacttt tgaggggaga ttatgaccac tgaatttgtg attggaatag
     1381 gaagagaggc gatacttata accttgatgg tttccgcgcc gatgctggtc tttggcttga
     1441 tcgtgggatt ggcggtaagt atacttcaag cagtaaccca ggtgcacgag atgaccttaa
     1501 ttttcattcc caagataatc gctgtggctc tggctctttt gattttcctg ccctggatac
     1561 tcaacaaggt agttgatttc actgctaaat tgataaatag catccccaat atcgcgatgt
     1621 aagtttacca ctttgaactt caaaaacaca agactgtttt aatgaatttt tggtcgaggt
     1681 tgggagacct cgacctactc tttttgtgga aaatccttag ggtttcttac agttctttgc
     1741 gcagttcggg cgaccacgag ggtcgcccct acccgaatcc ccgtcctttt tggaattgat
     1801 ttttggagga attttactat tatgcaaaac aaaggagggt aatgtggcta agaagacttt
     1861 ggttattgtt ctggttttgt tttttgttct ttcaggtgta agttggggga ttgagagggt
     1921 ggagaggaat cgtcgcggca catttttgtt tgttgatggt ggctggacaa ctctggacga
     1981 acatagttcg cgagatggtt tttctttagg tttcggaatt ggtggaaact tttcaggaca
     2041 tcacataggc agattcagtt ttcgatattg ccaatacgga tataagttta gccaaactgg
     2101 aagcaaatgg actggaaaga tttttgacta tggtttatct ctgaaaacaa ccataataga
     2161 tgtgcataga catccaattg caaatcctta ttttggtttt ggtctcggtc ttatgtatgc
     2221 tatttctaga tacgagtaca gtgatatcgt attttatcca taccaggtag agcaggactc
     2281 aaggtttttt atgagcttca acttttttac tggatgtgat tttgctttaa ccgagaagtc
     2341 atggcttttg ttggagggtg gttttcttat ggatattcaa gagcccaccc tctttcggga
     2401 tgaaaaggtg agtatacgtt ctagaatgga aaattacaga gtggtcattg gatttgggca
     2461 taagctttga tttgttcagt taatcccagt cctcgtaggc gacccatatg ggtcgcctac
//
LOCUS       DASWLN010000074         2642 bp    DNA     linear   ENV 20-FEB-2024
DEFINITION  MAG TPA_asm: Candidatus Zixiibacteriota bacterium isolate
            SMAG_U2949 k141_161240, whole genome shotgun sequence.
ACCESSION   DASWLN010000074 DASWLN010000000
VERSION     DASWLN010000074.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37960594
            Sequence Read Archive: SRR10376091
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Candidatus Zixiibacteriota bacterium (soil metagenome)
  ORGANISM  Candidatus Zixiibacteriota bacterium
            Bacteria.
REFERENCE   1  (bases 1 to 2642)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 2642)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (26-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/12/2024 08:28:26
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 1,440
            CDSs (total)                      :: 1,416
            Genes (coding)                    :: 1,412
            CDSs (with protein)               :: 1,412
            Genes (RNA)                       :: 24
            rRNAs                             :: 1 (16S)
            partial rRNAs                     :: 1 (16S)
            tRNAs                             :: 21
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 4
            CDSs (without protein)            :: 4
            Pseudo Genes (ambiguous residues) :: 0 of 4
            Pseudo Genes (frameshifted)       :: 0 of 4
            Pseudo Genes (incomplete)         :: 3 of 4
            Pseudo Genes (internal stop)      :: 1 of 4
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2642
                     /organism="Candidatus Zixiibacteriota bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_161240"
                     /isolate="SMAG_U2949"
                     /isolation_source="soil"
                     /db_xref="taxon:2053527"
                     /environmental_sample
                     /country="USA: Oregon"
                     /lat_lon="42.99 N 123.63 W"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            <1..629
                     /locus_tag="VGB16_02445"
     CDS             <1..629
                     /locus_tag="VGB16_02445"
                     /inference="COORDINATES: protein motif:HMM:NF019869.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004812 - aminoacyl-tRNA ligase activity
                     [Evidence IEA];
                     GO_process: GO:0006418 - tRNA aminoacylation for protein
                     translation [Evidence IEA]"
                     /codon_start=3
                     /transl_table=11
                     /product="class I tRNA ligase family protein"
                     /protein_id="HEX9916572.1"
                     /translation="NILRLLHPFIPFITEEIWYHLYAYPIDDTTKTIMRTEWPKKQSD
                     LIKPELEDVVDKVQKVVYAIRNIRSEMNIPPTVEIKVILKVDNRELLSILRENQQHIT
                     TLGRVDSLEMGARIKKPSWAASAVIKDVEIFVPLEGVVDVEKERARLEKEIVRVADLL
                     TKTNKKLSNEDFLKRAPGEIIEKEETKRGDYEKMIDKLNQNLERIMRW"
     gene            632..1993
                     /locus_tag="VGB16_02450"
     CDS             632..1993
                     /locus_tag="VGB16_02450"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEX9916573.1"
                     /translation="MKLKILLLGALLVLITWQVSLASSDVAITIYNQNLGLVRDVREA
                     NFVKGQNVFSFSDVASQIDPTSVHFKFLDAPEKVTILEQNYQYDLVDSRKILYKYLDK
                     EIQLFTKQDKLFEGGLLSFSSTDLILKEKNGGLRIVTFDDVRDLYFPSLPEGLITKPT
                     LVWLVNSENSLKGKAEVSYLTGGIDWHAEYVAVTDKDDKNLDLSGWVSIDNHSGATYE
                     NAKLKLMAGEVRRIEGRVLPTAYLAKGMALEGAGAEFEEKPFFEYHLYTLQRKTTLKD
                     NEIKQVSLFSPALVKAEKILTYDGARDVKKVRVNLEFVNSQKAGLGIPLPGGKIRVYK
                     KDVDGSLEFIGEDKIDHTPKDEKVRVYLGNAFDVVGERTKTDFRKITDKLSEETYRIK
                     LRNHKDQKTEVIVIEHFYSQWEITKANFEYVRKDATTMEAKISLDKDEEKVLEYTVRY
                     SYY"
     gene            2417..>2642
                     /locus_tag="VGB16_02455"
     CDS             2417..>2642
                     /locus_tag="VGB16_02455"
                     /inference="COORDINATES: protein motif:HMM:NF024806.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="tetratricopeptide repeat protein"
                     /protein_id="HEX9916574.1"
                     /translation="MKQFKYYLLALGFCFLIVLGCKSSYLRSAKIYLFDVKPPDYKNA
                     ITQLDKEMQANPNNPETYFLLGYIHYQQGKY"
BASE COUNT          843 a          485 c          634 g          680 t
ORIGIN      
        1 acaacatctt aagacttctc caccctttca ttccattcat aactgaggag atctggtatc
       61 atctctatgc ttacccgata gatgatacaa ccaaaaccat tatgcgaact gaatggccaa
      121 aaaagcaatc agacttaatc aaaccggagc tggaagacgt tgtagataaa gttcaaaaag
      181 tggtttatgc tataagaaat atccgctcgg agatgaatat ccccccgact gtcgaaataa
      241 aagttatact caaggttgac aaccgcgaat tgcttagtat actaagagag aatcagcagc
      301 atatcaccac tctgggcaga gtggatagcc tggaaatggg agcaagaatc aaaaagccga
      361 gctgggctgc atccgcagtg attaaagatg tggaaatctt tgttcctttg gagggggtag
      421 ttgatgtgga gaaggaaaga gcaagattgg aaaaggaaat agtgcgtgtg gctgaccttt
      481 tgacgaagac caataaaaag ctttcgaatg aggatttcct gaaaagggca cccggggaga
      541 ttatagaaaa agaagagact aaaagaggag attacgagaa aatgatagat aaactaaacc
      601 aaaacctgga aaggataatg aggtggtaaa tatgaaactt aaaatacttc tcttgggagc
      661 tttgttggtg ttgatcactt ggcaggtctc tttggcttca tccgatgtgg ccatcactat
      721 ttacaatcag aacctcggtt tagtgagaga tgtaagggaa gcgaactttg tgaagggaca
      781 aaacgtattc agcttttcgg acgtggcttc acagattgac cctacctcag ttcattttaa
      841 gttcttagat gctcctgaaa aggtgaccat tttggaacag aattaccaat atgacctggt
      901 tgattcacgc aagatactat acaaatattt ggacaaagag atacaacttt tcaccaagca
      961 ggacaaatta tttgaaggag ggcttctctc cttttcctcc actgatttaa tcctcaaaga
     1021 aaaaaatggc ggattaagga tagtaacctt tgacgatgtc agggaccttt attttccgag
     1081 cttgcccgaa ggtctaatca ccaagccgac tttagtctgg ttggtgaaca gcgaaaatag
     1141 tctcaaagga aaggcagaag tcagttatct gaccgggggg atagattggc atgctgaata
     1201 tgtggcggtg acggataagg acgacaaaaa tctggatttg tccggctggg tttcaattga
     1261 caatcattca ggtgcaactt acgaaaatgc taaattgaaa cttatggcag gagaggtaag
     1321 aagaatagag gggagggttt tgcccactgc ttacttagct aaggggatgg cattggaagg
     1381 agctggggcg gaatttgagg agaagccttt tttcgaatac cacctctaca ccttgcaaag
     1441 aaaaactacc ctgaaggata atgaaataaa gcaggtatcg ctcttttctc cggctttagt
     1501 caaagccgag aagatattga cttatgatgg agccagagat gtcaagaaag tcagggttaa
     1561 cttagaattt gtcaactcac agaaagcagg attaggaatt cctttgcctg gtgggaaaat
     1621 cagggtttat aagaaagatg tggatggctc gttggaattc atcggagagg ataaaatcga
     1681 ccataccccc aaggacgaga aggttagagt gtatctgggg aacgcctttg atgttgtggg
     1741 ggagagaaca aaaaccgatt ttcgcaaaat aacagacaaa ctaagcgagg agacctatcg
     1801 catcaaatta cggaatcaca aagaccaaaa aacagaagtg atagtgattg aacattttta
     1861 ttcccagtgg gagataacca aagccaattt cgaatatgtc aggaaggatg ctacaactat
     1921 ggaagccaag atctctttgg acaaagacga agaaaaagtt ttagaatata cggtgagata
     1981 cagttattac tgaggtacgc gaaaataatg gagattgtgt ctgtcgggtc ccctgacccg
     2041 acagacattt tccgacaggt cagcctgccc gtttcttccc ggtgtgaacc agaacggttc
     2101 acaccccgct cgacccaatg gccaggggga cctggcgggc acagaaaact ccgctcgcgt
     2161 tatttatgcg taagtcaata acaatttctt aaacaaagga gtcctatggt gctttgtaac
     2221 ataaatggag gttgcatccg tatacccacc ttgccccaag gtgggatgct tgtttccgtc
     2281 ccatgttgag ggcaacatgg gtttactcga taaatactga agaacctaaa ttatggtaat
     2341 atcatttagg aaacgatgca ctatggagtg atgtttttat ttaagcttac aaaacaactt
     2401 tttagaggag gagcaggtga aacaattcaa gtattatctg ttggctttgg ggttttgttt
     2461 tttgattgtg cttggctgta agagttctta ccttaggtca gctaagattt atctttttga
     2521 tgttaagccg ccggattaca agaacgccat cactcaattg gataaggaga tgcaagccaa
     2581 tccgaacaat cctgagacat attttcttct gggttacata cactatcagc agggcaaata
     2641 tg
//
LOCUS       DASWLN010000075        18205 bp    DNA     linear   ENV 20-FEB-2024
DEFINITION  MAG TPA_asm: Candidatus Zixiibacteriota bacterium isolate
            SMAG_U2949 k141_707899, whole genome shotgun sequence.
ACCESSION   DASWLN010000075 DASWLN010000000
VERSION     DASWLN010000075.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37960594
            Sequence Read Archive: SRR10376091
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Candidatus Zixiibacteriota bacterium (soil metagenome)
  ORGANISM  Candidatus Zixiibacteriota bacterium
            Bacteria.
REFERENCE   1  (bases 1 to 18205)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 18205)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (26-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/12/2024 08:28:26
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 1,440
            CDSs (total)                      :: 1,416
            Genes (coding)                    :: 1,412
            CDSs (with protein)               :: 1,412
            Genes (RNA)                       :: 24
            rRNAs                             :: 1 (16S)
            partial rRNAs                     :: 1 (16S)
            tRNAs                             :: 21
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 4
            CDSs (without protein)            :: 4
            Pseudo Genes (ambiguous residues) :: 0 of 4
            Pseudo Genes (frameshifted)       :: 0 of 4
            Pseudo Genes (incomplete)         :: 3 of 4
            Pseudo Genes (internal stop)      :: 1 of 4
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..18205
                     /organism="Candidatus Zixiibacteriota bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_707899"
                     /isolate="SMAG_U2949"
                     /isolation_source="soil"
                     /db_xref="taxon:2053527"
                     /environmental_sample
                     /country="USA: Oregon"
                     /lat_lon="42.99 N 123.63 W"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            <1..191
                     /locus_tag="VGB16_02460"
     CDS             <1..191
                     /locus_tag="VGB16_02460"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=3
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEX9916575.1"
                     /translation="SLLGKAKEAFEYLDRSIKFGYSDYEQMKKDTDFDFLRKSDPERF
                     KKIVAQTQKIARQKKPGK"
     gene            complement(1014..1727)
                     /locus_tag="VGB16_02465"
     CDS             complement(1014..1727)
                     /locus_tag="VGB16_02465"
                     /inference="COORDINATES: protein motif:HMM:NF014635.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF169 domain-containing protein"
                     /protein_id="HEX9916576.1"
                     /translation="MEWKEYSKRLKGLLKLKGSPIAVTYSMNPPKNYKKGKCRACDAL
                     VLARDGQTVNLTKESSSCRGGIWSLGLAPQPSGEAFKALQKFLVEGEKLCASIVVFHR
                     MRALNTPAPLGLADNVIVSPLEKSETRPDLVVFICNPEQVSRLVTLATFYDGIPPKLE
                     ITGSTCRMSIAYPIVSGQMNANFFDYTSRKIKGFGKDELIISIPYEKMDNLIKAIPNC
                     TAGTAKIEYPEEFRRLAGE"
     gene            1992..2252
                     /locus_tag="VGB16_02470"
     CDS             1992..2252
                     /locus_tag="VGB16_02470"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_012447936.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="4Fe-4S dicluster domain-containing protein"
                     /protein_id="HEX9916577.1"
                     /translation="MPDGAEQVMTEKEEQQKVHVFEAWCKKCGICVAFCPTKALTSDK
                     DGLPVLTYPEKCTQCGMCELRCPDFAIAVRPKEPKETKKESK"
     gene            2249..3400
                     /locus_tag="VGB16_02475"
     CDS             2249..3400
                     /locus_tag="VGB16_02475"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_020252330.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="2-oxoacid:acceptor oxidoreductase subunit alpha"
                     /protein_id="HEX9916578.1"
                     /translation="MNQDTKNIARGELLQGNEACARGAITAGVRFFAGYPITPSTEIA
                     EYLARNLPKVGGKFIQMEDELASMAAIIGASLTGTKAMTATSGPGFSLMQENIGYAYM
                     AEVPCVVVDVQRGGPSTGLPTKISQSDTMQARWGTHGDYYSIALAPSSVEECFTSTIQ
                     AVNLSEKYRTPVIVLMDEVIGHMREKVNLPDPKQIKIIDREKPTVPPDWYQHFEINPH
                     FVSPMASFGEGYRFNVTGLTHDPFGFPTGQPKEIADKLDKLKNKIMHHADDIIQVRDE
                     LMENANMAVFAYGAAARAARQAVRMARQKRIKVGLIQPLTIWPFPDTYLKSVLEQLDL
                     ILVVELNQGQLVLEVERVSCGKAMVKSLNRYDGELITPEQIFNRIKEVR"
     gene            3408..4232
                     /locus_tag="VGB16_02480"
     CDS             3408..4232
                     /locus_tag="VGB16_02480"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_012063759.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="2-oxoacid:ferredoxin oxidoreductase subunit
                     beta"
                     /protein_id="HEX9916579.1"
                     /translation="MYSEITHKYLRTKKRFPNVWCAGCGIGIALGAIIRAIDKLSLDR
                     NEVVMVSGIGCSSRMPVYVDFNTLHTTHGRALAFATGVKLAKPSMKVIVITGDGDALA
                     IGGNHFIHACRRNINITTILINNRIYGMTGGQYSPATPFMKFGTTAPYGNRERDFDIP
                     MLAQTAGAPFVARGTVYHVVQLQKLVEAAIVKKGFSLVEVISQCHTLYGRLNKEGGAV
                     EMMTWQKEHAVPIEAAKNLPPEKLKDKLLTGILWNVDIPEYCEEYDKIIAETKEVN"
     gene            4246..4815
                     /locus_tag="VGB16_02485"
     CDS             4246..4815
                     /locus_tag="VGB16_02485"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_013275999.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016903 - oxidoreductase activity, acting
                     on the aldehyde or oxo group of donors [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="2-oxoacid:acceptor oxidoreductase family
                     protein"
                     /protein_id="HEX9916580.1"
                     /translation="MKKSYMDSTDRYEIRLSGSGGQGLGFAGTILAEAVGVYHGKNVV
                     QTQSYGPEARGGASRSDIVISSGEIFYPKSMKLDLLLALTQKACDKFYTELKEGGILV
                     VDSGLVAQLPPVKVHAFPFTQLSREKVGTPMVANIMALGTVTELSKIAPREALIEAVR
                     KRAPKGTEERNLKALQIGFELPTKKNSKK"
     gene            4819..5223
                     /gene="ndk"
                     /locus_tag="VGB16_02490"
     CDS             4819..5223
                     /gene="ndk"
                     /locus_tag="VGB16_02490"
                     /EC_number="2.7.4.6"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_012662032.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004550 - nucleoside diphosphate kinase
                     activity [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="nucleoside-diphosphate kinase"
                     /protein_id="HEX9916581.1"
                     /translation="MPSKTLLFIKPDAVKRNLIGEIIKRIENAGFKVTALEMKKLSSK
                     RTKEFYKIHKDKPFFNQLVKSIANKPIVGMILEKKSAVMSLREFIGTTNPKKAKKGSI
                     RADFGLDVTRNSVHASDSPKNAKIETKIFFKK"
     gene            5788..7104
                     /locus_tag="VGB16_02495"
     CDS             5788..7104
                     /locus_tag="VGB16_02495"
                     /EC_number="2.3.3.1"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_006927567.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004108 - citrate (Si)-synthase activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="citrate (Si)-synthase, eukaryotic"
                     /protein_id="HEX9916582.1"
                     /translation="MMSLKERLAKKIVEKQEEVKYLLKEHGEKVISQVNIQQAYGGMR
                     EIKCMVWETSLLDPMEGIRFRGYSIPEVREKLPKAQGGEEPLPEGIFWLLMTGELPSK
                     SDVREITKEWHSRGEVPKETWKILNALPKNMHAMTQFSIGILSLQPTSLFTKRYEEGI
                     PKSEYWDPMYEDVMNLLAKLPAIAAYIYRRTYKDGKRIRPIKRLDWGANFAHMMGIDN
                     PQYDELMRLYLVLHCDHEGGNVSAHTTHLVGSALSDAFYSLSAGMDGLAGPLHGLANQ
                     EVLHWIQNLMKKLGGKLPTKEELCKALWDTLNCGQVIPGYGHAVLRKTDPRYIAHREF
                     ALKHMPEDDLFKLCSLIYETAPDILIKHGRAKNPWPNVDAHSGVLQWYYGVKEYDFYT
                     VLFGVSRAMGVLAQLVWDRALGFPLERPKSVTTEWIRKTVKEMQSS"
     gene            7252..8538
                     /locus_tag="VGB16_02500"
     CDS             7252..8538
                     /locus_tag="VGB16_02500"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_010936225.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="3-isopropylmalate dehydratase large subunit"
                     /protein_id="HEX9916583.1"
                     /translation="MGMTLAEKIISQHAGKNVKAGEIALVNVDVCLTQDGTGPLAIRQ
                     LQKIDLEKAANPKSTVLFIDHAAPSPRSELSNDHITIRKFAEKTGACLSDVGEGVCHQ
                     IIAESYLKPGDILIGADSHTCTGGALCAFSTGMGSTDVAIGIALGKTWLRVPETFKIE
                     VSGEFKKGVYAKDLILHLIGKIGADGATYKALEFCGETIENMNMSSRLTLANMAVEAG
                     AKAGLIASDKTTKKFLKEYGREKDFKAIKADNDAEYEKVIKIDASKLVPIVSFPHTVD
                     NTKTVDQAKGIEVDQIFIGTCTNGRLEDLEIFANIVKGKRKHSKTRLIVVPASKRVFL
                     DALDKGIIKTLVDFGAAVLAPGCGPCVGVHEGILGDGEICLSTMNRNFKGRMGNPKGF
                     IYLASPATCAVASVLGEIADPRRFVNKANSKSKKTK"
     gene            8567..9067
                     /locus_tag="VGB16_02505"
     CDS             8567..9067
                     /locus_tag="VGB16_02505"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_010879257.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="3-isopropylmalate dehydratase small subunit"
                     /protein_id="HEX9916584.1"
                     /translation="MLLTGKAWKFGDNISTDLIAPGRLFHLRSNLPELAKHVLEDADP
                     EFAKKVQVGDFVVGGNNFGLGSSREHAPTIIKLAGVSAVLAKSFARIFYRNAINVGLP
                     VLICDTDKIKNGDELEVDLEKGTVVNKITGDVLKFAPLPKAMIRILNDGGLLAHIQKY
                     GDFKID"
     gene            9078..10325
                     /gene="icd"
                     /locus_tag="VGB16_02510"
     CDS             9078..10325
                     /gene="icd"
                     /locus_tag="VGB16_02510"
                     /EC_number="1.1.1.42"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_013370350.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004450 - isocitrate dehydrogenase (NADP+)
                     activity [Evidence IEA];
                     GO_process: GO:0006099 - tricarboxylic acid cycle
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="isocitrate dehydrogenase (NADP(+))"
                     /protein_id="HEX9916585.1"
                     /translation="MIKFEKLVVPSEGDGIEVQNGELKIPDKPIIPAIEGDGIGRDIS
                     KAMRRVLDAAVEKYCGGKKKIVWFDIYAGEKAYEKYGQWLPKDTLEAIEHFIVAIKGP
                     LTTPVGGGFRSLNVTLRQVLNLYACVRPVRYFEGVPSPVKSPQDMNVVIFRENTEDVY
                     AGIEWAEGTKEAGEVISFLNSNFKLDIRTDSGIGIKPISITGTERLVRKAIRYALENS
                     RRSVTLVHKGNIMKYTEGAFRDWGYKLATREFRDKVITEDELANIPDCKVPPGKIVMK
                     DRIADSMFQQILTRTNEYDVLATPNLNGDYLSDACAAQVGGLGMAPGANIGDYVGLFE
                     ATHGTAPKYADKDMINPGSIILSGCMMLEYLKWKDAAEMIYSAIAKTIKQKKVTYDLH
                     RLMEGATKVSTSGFASAIIENMG"
     gene            10341..10934
                     /locus_tag="VGB16_02515"
     CDS             10341..10934
                     /locus_tag="VGB16_02515"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEX9916586.1"
                     /translation="MNKQEQQGWLDEHIPYRLQAIVSLRWVCELLVSENYPTSVKLYF
                     DNQEVLSNNSFRFLTNPMLEIGTIYCRVLLEFLGLSLNKGNRQLIERSSSHSDDIRIE
                     DFGLKPLTRKEVFKAPMGRPEEIEEACIYTISAANKAVVHLTRGFYDKTGIGRLHRCS
                     FVVPWLICEYLYKPLNLPDPEYKLGEPVFAPGLSPLK"
     gene            complement(11134..12006)
                     /locus_tag="VGB16_02520"
     CDS             complement(11134..12006)
                     /locus_tag="VGB16_02520"
                     /inference="COORDINATES: protein motif:HMM:NF013088.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0016020 - membrane [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="EamA family transporter"
                     /protein_id="HEX9916587.1"
                     /translation="MNKTSLGYLLVLIGATFTGTIGVMGKLIYAYEPSPLTVITFRAT
                     FAFLFIAFVLVVFRPGLLRINLTDLPYFALYGFLSITLSFMFFFNSIKYTTVATATIL
                     LYTFPAIVVLLSALILNEKITRYKIVALILTFLGTASVMQVYNTTKFNLNLKGALLGL
                     GAALGAASYTILGKRAVNKYDPLTVVFYAFGFGSLFLLLFRGPKTVLQVNYPILAWVW
                     ILILALISTLLGYFLYTKGLQYIEAGRASITAVWEVIVASVLAFIVLGEVLTLPQVAG
                     GLLIILGIFVIRHE"
     gene            complement(12068..12637)
                     /locus_tag="VGB16_02525"
     CDS             complement(12068..12637)
                     /locus_tag="VGB16_02525"
                     /EC_number="2.1.1.100"
                     /EC_number="2.1.1.334"
                     /inference="COORDINATES: protein motif:HMM:NF016107.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008168 - methyltransferase activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="isoprenylcysteine carboxylmethyltransferase
                     family protein"
                     /protein_id="HEX9916588.1"
                     /translation="MNFYIIIILISFVWILSEVILARITHSKTKTSKDLDKSSLRFLW
                     LTIIICVFIGVFLGIRGIGFISKNALFISSLGLILIVLGLVVRWTAILTLKKYFTVNV
                     SILSDHRIVDKGIYKIIRHPAYAGSLLSFLGLGLSFSNWLSTLIIFTPVLVVFIYRMR
                     IEEKALVGFFGEKYTIYCKTTKRLIPKIY"
     gene            complement(12845..14308)
                     /locus_tag="VGB16_02530"
     CDS             complement(12845..14308)
                     /locus_tag="VGB16_02530"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011700717.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="4-hydroxyphenylacetate 3-hydroxylase N-terminal
                     domain-containing protein"
                     /protein_id="HEX9916589.1"
                     /translation="MALRSAEQYKDSLKKMKLNVYKFGKLIGDVTTHPATKWTILGHS
                     QIFEAQNNPENQELFTTKSHLTGEKVSRYLSIIQSAEDMIANLKMKREMFHLSGTCTG
                     GRCVGWNCLNAMWAVTFDMDKDLATDYHQRLEKWLRYAQANDIAVAGALTDPKGDRTI
                     PPSQQEDKDMSLRMIEKREDGIVVKGAKVMICGVAASNEIFVMPGAAYKEQEGAYAIS
                     FVIPRDIEGLTIIETRRPSDTREEEEGFDIPVKVGGITQAYLLFENVFIPKDRIFMCG
                     EHKYALPAILYFVSLYRSAIGGCVAGQGDNKVGASALVARANGLSTKVFSEKFTQMYI
                     NNETTFGMGIAASTLGKKHLSGVWIPDTLLANINKVYVATLPYQTSVLAQDISGGIAE
                     TGCIPSYKDFTDPRYGKLLQKYLKAKSSAEARTKAARLVEWATIGAGVPGCMHGGGSP
                     DGAKLLIRANVNLERYVNIAKRLAGIEEEIAEPEKKK"
     gene            14601..15500
                     /locus_tag="VGB16_02535"
     CDS             14601..15500
                     /locus_tag="VGB16_02535"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_010345812.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0070403 - NAD+ binding [Evidence IEA];
                     GO_process: GO:0006631 - fatty acid metabolic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="3-hydroxyacyl-CoA dehydrogenase NAD-binding
                     domain-containing protein"
                     /protein_id="HEX9916590.1"
                     /translation="MAIKKVIVIGGGVMGRGISRLIAATGIEVILLDLNQKIVEQSMK
                     LIGEEMNAEIEKWGLTKSEKKAILSRIKPTVDLNQACGCDMLIEAIPEDLKSKQELFR
                     KIDKLCPKDAIMVTNTSTLSITEIASVTTHPEKVIGMHFLPPVTKIPLVEIVRGLKTS
                     RETFELIKKFAGEQLNKTVVSVYEYPGYVTTRIIVPLLNEAMYVLMEGIASAEDIDTA
                     MKLGYGFDIGPLALADMMGLDEVMSWMENLLAELSEHKYNPCPLLRKLVRAGHLGKKT
                     GQGFFKYDQEGNKIGEAISFIKW"
     gene            15505..16737
                     /locus_tag="VGB16_02540"
     CDS             15505..16737
                     /locus_tag="VGB16_02540"
                     /EC_number="2.7.2.1"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_006927215.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0000287 - magnesium ion binding [Evidence
                     IEA];
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_function: GO:0008776 - acetate kinase activity
                     [Evidence IEA];
                     GO_process: GO:0006085 - acetyl-CoA biosynthetic process
                     [Evidence IEA];
                     GO_process: GO:0016310 - phosphorylation [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="acetate kinase"
                     /protein_id="HEX9916591.1"
                     /translation="MKILVLNCGSSSVKYQLIDMDNDLVLAKGLVSRIGMSGAVLSHK
                     PHDRNEVKISGEMLDHIVAVEHVIAILLSPNHGVIKDKSEISAVGHRVVHGGEKFPDS
                     VFITPEVMLELRNCIDLAPLHNPHNIRGINACQRILPGVPQVAVFDTAFHQKMPPVAY
                     IYGIPYVLYKRYGIRRYGFHGTSHFYVSQRTSQLMNKSIKELKIITCHLGNGASMAAV
                     DQGISVDTTMGFTPLEGLLMGTRSGDLDPAIILHIMAREELSLHEANTLLNKHSGIQG
                     ISGISSDMRDIIEESSKGNAGAKLAFDIYCYRIRKYIGAYAATMGGLDVLVFTAGVGE
                     NAPLVRKASCENLEFLGVKIDSQKNDQMIGKEGIISTDDSRVKVLVIPTNEELVIAQD
                     TERIVEEQARPRETSRGL"
     gene            16751..17572
                     /locus_tag="VGB16_02545"
     CDS             16751..17572
                     /locus_tag="VGB16_02545"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011344884.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="sulfide/dihydroorotate dehydrogenase-like
                     FAD/NAD-binding protein"
                     /protein_id="HEX9916592.1"
                     /translation="MNEIIFQENLAPQINVIKLKLPHIVNKAKPGQFVILRINEKGER
                     IPMSIADLNKEDGILTIVFQEVGKTTAQLGTLRKGDFLADVVGPLGIPTHIEKFGHCV
                     CIGGGIGIAPIHPIARALKQAGNQVTSIIGARSKNLLIMQEEVNEASHKVLPSTDDGT
                     YGRKGFVTDILTELLIHKNKIDYIMAIGPVIMMKAVCNITKPYGIKTMVSLNPIMVDG
                     TGMCGACQVTVEGMTKFACVDGPDFDGHKVDFEELIKRQHMYLKQERTAMERYNK"
     gene            17585..>18205
                     /locus_tag="VGB16_02550"
     CDS             17585..>18205
                     /locus_tag="VGB16_02550"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_015949085.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="NAD(P)-binding protein"
                     /protein_id="HEX9916593.1"
                     /translation="MAISGQGAKIPRQKMMEQKPGDRIRNFNEVPFGYTSEMAQVEAS
                     RCLQCKKPICISGCPVEIDIPTFLKLVAQGDFIGAAKKLKEANSLPAVCGRVCPQEEQ
                     CERICVLTKKFQSVGIGNLERFAADFEREQGEVEIPKIEKSTGKRVAIVGSGPAGLTV
                     AGDLIKMGHQVTIFEALHKAGGVLVYGIPEFRLPKAIVQAEVDYLLK"
BASE COUNT         5522 a         3620 c         4292 g         4771 t
ORIGIN      
        1 attctctgct cggtaaagcc aaagaggcat ttgaatacct tgatcgctcc atcaagttcg
       61 gttattctga ttacgagcag atgaagaagg atactgattt tgattttctg cggaagagtg
      121 atccagaaag atttaaaaag atagttgctc aaacacagaa gattgcaaga cagaagaaac
      181 cgggtaagta atggctgttg ccattacttg attttgggaa cggcgtcccg caaagacggg
      241 gacgagacag ccgttcccta cccagataac ggcagaagca gtttctccta tcattgttat
      301 ctttaaaccg tatccccacc ttgccctcaa ggtggggttt tgtacctaac ccgcattgtg
      361 aaggcaatgt taagagcttt tcgtgtgccc gtcgagtgtc ccagctgaac aacctgaatg
      421 atataggtgg actcccccta ccaaaattcg cccaataaat tgggcaacta caatctcaaa
      481 tctcaaaaca cgagacttaa aactatttta gtgtagtcgc tcgatttatc gagcgttttt
      541 aggccttata taaaaaaaga aaaccgcagg agtagctcct gcggtaaaac atttaattgc
      601 aacgggaact ccccacctgt aaaggtgggg atgaaagcag tttgtaagtt tttgtgttgt
      661 tcctgtccct tcattcgcag ggatggactc cagtctcaag acacagaatt cgtgcaggaa
      721 tccagaaggg aaaagcaagc atggatcccc gattaaaccc tcggggatga caaaatacag
      781 taggggcaat tcatgaattg cccctacaaa caataagaag cacgcctgtc cttcgacatg
      841 ctcaggcctt cggctctgag gctcaggctc gaagagatgg tgacagttcg acttcgctca
      901 ctgtccctga gcagcgtcga agggagcaag gtcgaaccat aaacacgtgg gaatctacgt
      961 aggcgacatt cgcgaatgtc gcctacgttt cttaacgggt acaaagaata tttctattcc
     1021 cctgccaatc tcctgaactc ttccggatat tctattttag ctgtcccggc ggtgcagtta
     1081 ggtatggctt taatcaaatt atccatcttc tcataaggaa tactaattat caactcgtcc
     1141 ttgccaaatc cttttatctt tcttgaagta taatcaaaga agttcgcgtt catctgtccg
     1201 gatacaatcg gataagcaat agacatacga caggtcgagc cggtgatttc caatttaggc
     1261 gggatcccat cataaaaggt tgccagggtc acaagtcggg atacctgctc gggattacag
     1321 ataaatacca ccaaatctgg tctggtttct gatttctcta aaggtgaaac tatcacatta
     1381 tcagctaaac ccagaggtgc aggggtattc agggctctca ttctgtggaa aaccacaata
     1441 gaagcacaga gcttctcacc ctcaaccagg aatttctgta aagccttgaa tgcctcaccg
     1501 cttggctgtg gggcaaggcc caggctccag attccacccc tgcaactcga gctttccttg
     1561 gtcaggttga cggtttggcc gtctcttgcc aaaaccaaag catcgcaagc ccggcacttt
     1621 ccctttttat aatttttggg tggattcata gaataggtaa ctgcaatcgg gctgcccttg
     1681 agtttcaata gccccttcaa tcttttagaa tactctttcc attccatgat tggctccttg
     1741 tgtttaagat tagatgggag atttgagtta ttttagtctc acacttcatt ctatttgtca
     1801 ccagcaaaat acttcttttt gaaatataaa gcaacgttta ccagactcat ctctccattg
     1861 cttgtgaaaa aaataacaaa attgttccta aaatccttga cttttggtca acttgtagtt
     1921 tattatatct gttttttgaa gtcaagtttt ttctgtcttc gtcgtatcta tactaagagg
     1981 aaggtgataa tttgccggat ggggcagaac aggttatgac agaaaaagag gaacagcaaa
     2041 aagtccacgt ttttgaagcc tggtgcaaga aatgtggaat atgcgtagct ttttgtccaa
     2101 ccaaggcttt aacttcagac aaagatggtt tgccagttct gacttatccg gagaagtgca
     2161 cccaatgcgg gatgtgcgaa ttaagatgtc ctgattttgc aatcgcggtt aggcccaagg
     2221 aacccaagga aaccaaaaag gaatccaagt gaatcaagat acaaaaaata ttgcgagagg
     2281 ggagttatta cagggaaatg aagcttgtgc cagaggggcg ataactgccg gagtccgctt
     2341 ttttgccggc tatcccataa ctccctcaac tgagatagct gaatatctgg cgcgaaacct
     2401 gcccaaggtg ggcggaaaat tcatccagat ggaagacgaa cttgccagca tggcggcaat
     2461 aattggtgcc tccctaaccg ggacaaaagc tatgaccgcc accagtgggc cgggtttttc
     2521 cctgatgcag gagaacatag gatacgccta tatggcagag gttccctgtg tggtggtaga
     2581 tgtacaaagg ggcggaccct caaccggtct tccgaccaag atttcacaat cagatactat
     2641 gcaggcccgt tggggaacgc acggagatta ttattccatt gcgttggccc cctcttcagt
     2701 ggaagaatgt ttcacctcta ccatccaagc agtaaatctt tccgaaaaat atcgtacccc
     2761 ggttatcgtg ctgatggatg aagtaattgg acatatgaga gaaaaagtga atctgcccga
     2821 cccgaagcaa atcaagataa tagaccgaga aaaaccaact gtgcctccgg actggtatca
     2881 gcatttcgag ataaaccccc atttcgtttc gcctatggcg agttttgggg aggggtatcg
     2941 tttcaatgtt accggactta cccacgaccc gttcggtttt cccactggcc agccaaaaga
     3001 gatagcagat aagctcgata aacttaagaa caaaattatg catcacgcag atgatataat
     3061 tcaggtcaga gatgaactta tggagaatgc caacatggcc gtcttcgctt atggggcggc
     3121 tgcgcgtgcg gcgcgtcaag cagtgagaat ggctcgacag aagaggataa aagtgggctt
     3181 gattcaaccc ttgaccatct ggccctttcc ggatacctat ttgaaaagtg tcttggagca
     3241 gttagacttg attttagtag tggaattaaa tcagggtcaa cttgtgcttg aagtggagcg
     3301 ggttagttgt ggaaaggcaa tggtgaagtc gctcaataga tacgacggag aattgattac
     3361 cccggaacag atattcaatc gcattaagga ggtaagatag gttggaaatt tactccgaaa
     3421 taactcataa atacctgcgt accaaaaaga ggtttcccaa tgtctggtgt gctggatgtg
     3481 gcattggaat agccctggga gcaattataa gagccataga taagttaagt ttggatcgga
     3541 atgaagtggt tatggtttca ggcataggtt gttccagccg gatgccagtc tacgttgatt
     3601 tcaataccct gcacactact cacggaaggg ctttggcttt tgccaccggc gttaagctgg
     3661 ctaaacccag catgaaagtg atagtcatca ccggggacgg tgatgcccta gccattggcg
     3721 gcaatcactt cattcatgct tgccggcgca acatcaacat cactaccatc ttgataaata
     3781 atcgcattta tggcatgacc ggaggacaat actcccctgc cactcccttt atgaaatttg
     3841 gcaccaccgc tccctatggc aatcgggaaa gagatttcga tatccctatg ttagcccaga
     3901 cagccggagc gccctttgtt gcgcgaggaa cagtatatca cgtggttcaa ctgcagaaac
     3961 tagtagaggc ggctattgtt aaaaagggtt tttcgttggt ggaagtcatc agccaatgcc
     4021 acacccttta tgggagattg aataaggaag gtggggcagt ggagatgatg acctggcaaa
     4081 aggaacatgc tgtgccaatt gaagctgcaa agaatctacc tcctgagaag ctgaaagaca
     4141 aactacttac cgggatactg tggaatgttg atattccgga atactgcgag gaatacgata
     4201 agattatagc tgagaccaaa gaggtcaact gaaggagaac agaaattgaa gaaatcttat
     4261 atggattcaa ctgacagata tgaaattcgt ctctctggtt caggtggaca ggggttgggt
     4321 tttgccggaa ccattctggc ggaagcggtt ggagtttatc acggcaagaa cgttgttcaa
     4381 acccaaagtt atggacccga agccagagga ggagccagcc gctcagacat cgtgataagc
     4441 tccggagaga tattctatcc caagtcaatg aaattggatt tgcttttggc tttaacccaa
     4501 aaggcttgcg ataagttcta tacagaactg aaggaaggtg gaatcctggt ggttgattcg
     4561 ggtttagtcg ctcagcttcc acccgtaaaa gtccatgctt tcccctttac ccaactttcc
     4621 cgggagaagg tgggaactcc tatggtagcg aacattatgg ctttgggaac cgtgactgaa
     4681 ctaagcaaga tagctccgcg ggaagccctg atagaggcag tcaggaaaag agctcctaaa
     4741 ggaaccgaag aaagaaatct aaaagcttta cagataggat ttgagttacc caccaaaaag
     4801 aattctaaaa aataacccat gccctccaag acccttcttt ttataaaacc agatgcagtc
     4861 aaaagaaacc ttattggaga gataataaag cgaattgaaa atgccgggtt taaagttaca
     4921 gctttagaga tgaagaaact ctcctcaaag agaaccaaag agttttataa aatccacaag
     4981 gataaaccat tcttcaacca attggtgaaa tcaattgcaa ataagccaat cgtcggcatg
     5041 atattagaaa agaagagtgc agttatgtcc ttaagggagt tcattggaac aactaacccg
     5101 aaaaaagcaa aaaaggggag cataagggca gacttcggtt tggatgttac cagaaattct
     5161 gttcacgcct cagattctcc taagaacgcc aagatagaaa caaagatatt tttcaaaaag
     5221 taacattgtg tggagctgat aaaattcggt gagtggtgcg atggataccg aaaagcagtt
     5281 gacaatttgg aggaaatgaa gaaaatcggt gcaaagaggt ggccggaaaa acagatttag
     5341 tttagtaact atcatataat atttttcaag taggggcgta ttgcaatatg caagtacgcc
     5401 cctacatttt tttgctttga gagatacgca ggcgacccat ccgggtcgcc tgcagttttt
     5461 tcattcttca acctgcaatc ttattaggca cattcaatca aacgctattc tgattaagta
     5521 gaatatagat agttttgaga tgttaagtta aggttgtgta gtaaattaca acacaaaata
     5581 cctatcaaag cagaaaatgt ctacacccaa agaataataa actgttgtat tttgctacaa
     5641 aaatggaaga attcttaaga aagtatttga cagaacccaa agagagattt aattttaatt
     5701 cagtgttttc cgtataaaaa ggagaaaaga aaataaactt tttaaaccca ctcgtgaaat
     5761 aatttacaga atttaagaaa aggagttatt atgtctttaa aagaaaggct ggccaaaaaa
     5821 attgtagaaa aacaagagga ggttaaatat cttttgaagg aacacgggga gaaggtaatt
     5881 tctcaagtaa atattcagca agcctatggc ggtatgagag agataaaatg tatggtctgg
     5941 gaaacctctt tactggaccc gatggaaggg ataaggtttc gaggatatag tatccccgaa
     6001 gttagggaaa aactacccaa ggcacagggt ggagaggagc ctttgcctga aggcattttc
     6061 tggttgctta tgaccgggga acttccctca aaaagtgatg tcagggaaat tacaaaagag
     6121 tggcacagtc ggggcgaagt acctaaagag acttggaaaa tactgaacgc tttacctaaa
     6181 aatatgcatg ctatgaccca gttctccatc ggaattttat ctcttcagcc cacctcttta
     6241 tttactaaaa ggtatgagga aggaattcca aaaagtgaat attgggatcc gatgtatgaa
     6301 gatgtaatga accttttggc taaactcccg gctattgccg cctatatata tcgtcgaact
     6361 tataaagatg gtaaaagaat tcgtcccatc aagagattag attggggtgc aaatttcgcc
     6421 catatgatgg gcatcgataa tccccagtat gatgaattga tgaggttgta tctggtttta
     6481 cattgtgacc atgagggcgg aaatgttagc gcccacacta cccatttagt cggctccgcc
     6541 ctatcagatg ctttttattc tctttccgct ggaatggatg gtttagccgg ccccttgcat
     6601 ggtctggcga atcaagaagt cctacattgg attcaaaact taatgaagaa attgggtgga
     6661 aagcttccca ctaaagagga gttgtgcaaa gctttgtggg ataccttgaa ctgcggacag
     6721 gtcattcctg gttatgggca tgcagtctta agaaaaacag atccgagata catagcccat
     6781 cgagagtttg ctttaaagca tatgccagag gacgatcttt ttaaactgtg tagcttgata
     6841 tatgagaccg cacctgatat tctcataaaa cacggtagag ccaagaaccc ctggcccaat
     6901 gtggatgcac attcaggggt cttgcagtgg tattatgggg ttaaggaata tgatttctat
     6961 acagttttgt ttggggtttc ccgtgctatg ggagttttgg cgcaattggt ttgggatagg
     7021 gctttaggtt ttccattaga aagaccgaag tcggtaacta cggagtggat taggaagacg
     7081 gtgaaggaga tgcagagcag ttaatagttc acggttcata gtacaattct caatatttgc
     7141 gagaatgaca agcaaaaaat acggcaacca taacttagga cgttttcgaa atcgctcaat
     7201 taattgggca actgtcaaga aagcacaaaa agtctcaaat ggtgttttta tatgggaatg
     7261 acgttagccg aaaaaatcat tagccagcat gcaggaaaaa atgtaaaagc cggggagata
     7321 gccttggtga atgtggatgt ttgcttaact caagatggaa caggtccttt agccataagg
     7381 caacttcaaa aaatagattt ggaaaaagcg gctaatccga aatccactgt gctttttatc
     7441 gaccatgcag ctccttctcc gcgaagtgag ttatctaacg accatattac cattcgaaag
     7501 ttcgctgaaa aaactggtgc ttgcctttca gatgtgggcg aaggggtatg ccatcaaatc
     7561 atagctgaat catatctaaa acctggtgat atcttaattg gagctgattc gcatacttgc
     7621 actggtggtg ccttgtgtgc tttttcaacc ggaatgggct caacggatgt ggcaatagga
     7681 atcgccttag gaaaaacctg gcttagagtt ccagaaactt tcaaaatcga agtaagtggt
     7741 gaatttaaaa agggagttta cgccaaggat ttgattttgc acctgattgg taaaatcggt
     7801 gcggacggtg caacttataa agccctggag ttctgcggtg aaactattga aaatatgaat
     7861 atgagcagtc gtttgacttt ggctaatatg gcagtcgagg ccggggcgaa agctggactt
     7921 atcgcctcag ataagaccac taagaagttt ctaaaagaat atggcaggga aaaagatttt
     7981 aaagccataa aggcagataa cgatgcagaa tatgaaaaag ttataaagat agacgcttct
     8041 aaactggtgc caatagtatc attcccccac acagtggata acaccaaaac cgtagaccaa
     8101 gctaaaggaa tcgaagttga ccagatcttc atcggaactt gcactaatgg aagattggaa
     8161 gatttagaga tttttgccaa tatcgtcaaa ggaaaaagga aacactccaa aaccagactt
     8221 atagttgtcc ctgcttcaaa gagagttttc ctggatgctt tggataaggg cataatcaag
     8281 actttggttg atttcggtgc tgctgtgtta gccccaggat gtggtccgtg cgttggggtg
     8341 catgaaggca tattagggga tggcgagata tgcctctcca ctatgaatag aaatttcaaa
     8401 ggaagaatgg ggaatcccaa aggttttatt tacttggctt cacctgcaac ctgcgcggtc
     8461 gcttcagtat tgggcgagat agctgacccc agaaggtttg taaataaagc aaattcaaaa
     8521 agtaaaaaga caaaatgaaa atggaagata aataaggaag gcagttatgt tattgactgg
     8581 aaaagcttgg aaatttggag ataatatctc cactgattta atcgcgccag gaaggctttt
     8641 tcaccttcgc tcaaaccttc cggaattagc taaacatgtt ctggaggatg ctgacccgga
     8701 atttgccaaa aaggttcaag tgggcgattt cgtagtggga ggaaataatt ttggcttggg
     8761 ctcctcgcgc gaacatgctc caaccataat caaattggcc ggggtttcag cggtcttagc
     8821 taaatctttt gcccgcatat tttatcgcaa tgcaataaat gtggggttgc cggttttaat
     8881 ttgcgataca gataagataa aaaatggaga tgagctggag gtggatttgg agaaggggac
     8941 agtcgtcaat aagatcacgg gtgacgtttt gaaattcgcc ccccttccca aagcaatgat
     9001 tcgtatatta aacgatggtg ggttattggc gcacattcaa aaatatgggg atttcaaaat
     9061 agattagagg aggcagaatg ataaagttcg agaagttagt tgttcccagc gagggtgatg
     9121 gcatagaagt tcagaatgga gaactcaaaa tcccggataa gcctattatt ccggcgatag
     9181 agggggatgg aatcggcaga gatatctcaa aagctatgcg tcgggttttg gatgcagcag
     9241 tagagaagta ctgtggaggc aagaagaaga ttgtctggtt cgacatttat gccggtgaga
     9301 aagcgtatga gaaatatggc caatggctgc ccaaagatac gttggaggca atagagcatt
     9361 tcatcgtggc gattaagggt cctttgacca caccggtggg tggaggtttt cgcagcttaa
     9421 atgtaaccct caggcaggtt ctgaatctct acgcctgtgt cagaccagtg cgctattttg
     9481 agggtgttcc ttctccagtc aagagtcctc aggatatgaa tgtggtcatc ttcagggaga
     9541 ataccgagga tgtttatgca ggaatagagt gggctgaagg aactaaagaa gccggagaag
     9601 taatctcatt cttaaatagc aatttcaaat tagatataag gactgattcc ggaattggca
     9661 ttaagcctat ctctatcacc ggcactgaaa gactggtgcg taaggcaatc agatatgctc
     9721 tggagaatag cagaagatca gttactttag tgcataaggg taacattatg aaatatactg
     9781 aaggtgcttt ccgggattgg gggtataaat tggctaccag agaattcagg gacaaggtga
     9841 taactgaaga tgagcttgcc aatatcccgg attgcaaggt tccgccaggc aagatagtaa
     9901 tgaaagacag gattgcagac agtatgttcc agcagatttt gacccgaaca aacgaatacg
     9961 atgtgctggc aactccgaac ctgaatggag attatctctc cgatgcctgt gccgcgcagg
    10021 taggagggtt gggaatggca cccggggcaa atatcggaga ttatgtggga cttttcgaag
    10081 ctacgcacgg aactgcccct aagtacgcag ataaggatat gattaatccc ggctcaatta
    10141 ttctttccgg gtgtatgatg ttggaatatc tgaaatggaa agatgcggcg gagatgattt
    10201 attctgctat tgccaagacc ataaagcaga agaaagtcac ttatgacctg catcgcttga
    10261 tggaaggggc aaccaaggtc tccacttcag gatttgccag tgcgataatt gagaatatgg
    10321 gataagataa attgtgaaat atgaataaac aagagcaaca aggctggcta gacgagcaca
    10381 taccttaccg tcttcaggca atagtcagtc tacgttgggt atgtgagcta cttgtatcag
    10441 aaaactaccc aacatccgta aagttatact tcgacaatca agaggttttg agcaacaatt
    10501 catttcgttt cttgacgaat ccgatgttag agataggcac aatctactgc cgtgttttgc
    10561 ttgaatttct gggtctatcg ttaaataagg gaaatcgtca attaatagag agaagctctt
    10621 ctcatagcga tgacatcaga attgaggatt ttggattaaa gccactaaca agaaaagaag
    10681 tgttcaaggc accaatgggg agacccgaag aaatcgagga agcctgcatt tataccattt
    10741 ctgcagcaaa caaagctgtt gtccatctta ctcgtgggtt ttatgataaa acaggcattg
    10801 gacgtctaca tagatgtagc tttgtcgtcc cgtggttaat atgcgagtat ttgtacaagc
    10861 ctttaaatct gccagacccg gaatataaac ttggagaacc agtttttgcc ccaggtttga
    10921 gtccactgaa atagtccgcg cagcttctga atgtggttag acggtgtgcc caacttgtaa
    10981 aaatgacctc tcattctatt ctttttatgc tgtcattccc gcgaaagcgg gaatctattt
    11041 tttgaaagcg gtggattcct gcctgcgcag gaatgacaaa aaggatacaa agccattttc
    11101 gcaggcgaca ttggccaatg tcgcctgcat attttattcg tgtcttatca caaaaatccc
    11161 caaaataatc agcaatcctc cagcaacctg aggtagagtt aaaacttctc ccaaaacgat
    11221 aaatgccaaa acggaagcaa cgataacttc ccaaaccgca gttatgcttg ctcttccagc
    11281 ttcgatatac tgcaatccct tagtataaag aaaataaccc agaagggttg aaatcaaagc
    11341 taaaatgaga atccagaccc aagctaaaat cggatagttc acttgcagaa cggttttagg
    11401 acccctaaat aacaacagga acaaagagcc aaaaccgaac gcataaaaga caacggtcaa
    11461 ggggtcatat ttgttaactg ctctttttcc gagaatagta taagatgctg cccccagtgc
    11521 tgctcccaag cccaacagag cccctttcaa attgagattg aacttggttg tgttataaac
    11581 ctgcatcacc gaggctgttc ccaaaaatgt taatattaaa gcaacaatct tatatctggt
    11641 tatcttttca ttcaaaatca gggctgacaa caagaccaca atagctggaa aagtgtaaag
    11701 caagatagta gcggtggcaa cagtagtgta tttgatggag ttaaaaaaga acataaagga
    11761 gagggtgatg cttaaaaacc cgtaaagggc aaaatagggc aaatccgtga gattaattct
    11821 caataaaccc ggtctaaaaa ctaccaaaac aaaggcaata aacagaaagg caaaggtggc
    11881 gcggaaagtt ataacggtca aggggctagg ttcataagca tagatcaact tccccatcac
    11941 cccgatggtt ccggtgaaag tagcaccgat taaaactaaa agatagccca aagaggtttt
    12001 attcatttct tgcgcctgaa tggttcaggt atattttttt aattgggaga cgacgatttc
    12061 tcagcaatca atatatctta ggaattaatc gtttggtggt tttgcagtaa atggtgtatt
    12121 tctctccaaa aaacccaaca agggctttct cttcaattcg cattctgtaa atgaaaacca
    12181 ccaatacggg tgtgaatatg ataagcgtgc ttaaccaatt tgagaaagat aatcctaatc
    12241 caagaaatga gagcaggctt ccagcatatg ccgggtgacg aataatcttg taaattccct
    12301 tatcgacgat tcggtggtcg ctcaagatag atacatttac agtaaaatat tttttcaaag
    12361 taagaatagc tgtccatctg acgaccagtc ccaaaacgat taagatcaag cctaaacttg
    12421 aaatgaaaag agcattcttt gatatgaacc caatgccccg aatgcccaga aaaactccaa
    12481 taaaaacaca aataattata gtcagccaca agaatcttaa agaggattta tccaaatcct
    12541 ttgaagtttt tgtcttggaa tgcgttattc ttgctaatat gacttccgag agtatccata
    12601 caaaggaaat gagaattatt attatgtaga aattcatttt tatgaaaggt atgtcagaat
    12661 gtcctcgcag ggggataaat gacatttcta taagttccct ctcccttggg ggagagggtt
    12721 agggtgaggg ggatagcaaa gatggttctc accctctctc cgcctttggc gggattttca
    12781 acttaatcct ccccctcaag ggggaggaga tgtttcggat gccccatcct ttgggcgaga
    12841 aagttcactt tttcttctcc ggttctgcga tttcctcttc gatgccggct aatcttttgg
    12901 cgatgttcac atatctttcc aaattgacat ttgcccgaat caagagcttt gcgccgtcag
    12961 gagagccacc gccatgcata catcccggaa caccagctcc gatggtagcc cactccacca
    13021 gacgcgctgc tttggtacga gcctcggcag aggatttggc tttgagatat ttttgcagta
    13081 atttgccata cctggggtca gtaaaatcct tgtaggatgg aatacatccg gtttcagcaa
    13141 ttccaccgga gatatcctga gccagaaccg aagtttgata gggaagagta gcaacataaa
    13201 ccttgttgat attagctaaa agggtatccg gaatccagac tccggaaaga tgctttttac
    13261 ctaaagttga ggcggcaatc cccattccga aagtggtctc gttgttgata tacatctgag
    13321 tgaatttctc actaaagact ttagtggaaa gaccattggc tcgagctact aatgccgagg
    13381 caccaacttt attatctccc tgaccagcca cgcacccgcc aatggcagag cgatagagag
    13441 aaacaaaata gagaatagct ggcaaggcgt atttgtgttc tccgcacata aagattctat
    13501 ctttgggaat aaagacgttt tcgaagagaa ggtaagcttg agttatgcct ccaactttca
    13561 ctggaatatc aaacccttct tcctcttctc tggtgtcgct gggtcttctg gtttcgataa
    13621 tagttaaacc ttcgatatcc ctgggaatca caaaagagat ggcataagcc ccctcttgct
    13681 ctttataagc cgcaccgggc ataacgaata tctcattgga agcagccacc ccacagatca
    13741 tcaccttagc accttttacc acaattccat cttctctttt ctcgatcata cgcaaactca
    13801 tatctttgtc ctcttgctga gaagggggaa tagttctatc accctttggg tcagtcaaag
    13861 ctccagctac ggcgatatcg tttgcttgag catatcttaa ccatttttct aatctctggt
    13921 gataatctgt ggccaaatcc ttgtccatat cgaacgttac tgcccacata gcattcaaac
    13981 agttccatcc aacacatctg ccaccagtac aggttccgga aagatgaaac atctcccttt
    14041 tcatcttcaa attggcaatc atatcctctg cggactggat tatagataga tagcgggaaa
    14101 ccttttcccc ggtcagatgc gattttgtgg taaaaagctc ctgattttct ggattattct
    14161 gtgcctcaaa aatctgggaa tgccctaaaa ttgtccattt tgtggctggg tgcgtggtta
    14221 catccccaat caattttccg aacttataga cattgagctt catcttcttc aaactatctt
    14281 tgtattgctc cgcactcctt aatgccattt tgacctcctt aatttttctc gtatttcctg
    14341 taaacttact aaatgtgcaa atttttgcaa gcaaattccg ttctttttat aaaaacttcc
    14401 cataagagaa tagaggattt caaaaaggtg gtaacacaaa aatatgactt aggaaagcgg
    14461 tgtcaatttt ggcaccgctt tttgttttgc ggacttgtta ttttgttcac aaatcttttc
    14521 aaaaattcct tgcaaaatcc gattagttta ttattttaag gtttcgtcag gtttaactcg
    14581 tatagaaatt ggagaaaagt atggccatta agaaggtgat agttattggg ggtggggtta
    14641 tgggacgtgg aatatctcga ttgatagctg ctaccgggat agaagttatc cttctggatt
    14701 tgaatcagaa gattgtggaa cagagtatga aactaatagg cgaagagatg aatgcggaga
    14761 tagagaagtg ggggttgacc aaaagcgaaa agaaagccat tctttcgcgc atcaagccaa
    14821 cagttgacct aaatcaggcc tgcggttgcg atatgttgat cgaagccatc ccagaagact
    14881 taaaatcaaa acaggagtta ttcagaaaga tcgataagct ttgccccaaa gatgccatta
    14941 tggtgaccaa tacctccacc ctctccataa ccgagatagc ttcagtaacc actcatccgg
    15001 aaaaggtaat cggaatgcac ttcctccctc cagtaactaa aattccatta gtggagatcg
    15061 ttcgcggttt gaaaacttcc agagagactt ttgagctaat caaaaagttc gctggggagc
    15121 agttgaacaa gaccgtggtc tcagtttatg aatatcctgg ttatgtcacc actcggataa
    15181 tcgttcccct cctgaacgag gcgatgtatg ttctgatgga gggaatcgcc tcggctgagg
    15241 atatcgatac cgccatgaaa ttgggatatg ggtttgatat cggacctttg gctttagccg
    15301 atatgatggg tttggacgaa gttatgagtt ggatggaaaa tctcttagcg gagctttccg
    15361 agcataagta caatccttgt cctctgctac gaaagttggt ccgggcaggc catttgggaa
    15421 aaaagacggg acagggtttt ttcaagtacg atcaagaggg aaacaaaata ggagaagcaa
    15481 tctcttttat caaatggtga gaatatgaaa atattagttc tgaattgtgg cagctcctcg
    15541 gttaaatacc aactcataga tatggataat gatttggtac tggctaaggg tttggtctct
    15601 aggatcggga tgagcggagc agttttgagt cataaaccgc atgatagaaa tgaggtcaag
    15661 atatccgggg agatgttaga ccatattgtg gcggttgagc atgtgatagc aattttactt
    15721 tcacctaatc acggagtgat aaaagataag tctgaaatct cggcagtggg ccatcgggta
    15781 gtgcatgggg gtgagaagtt tcctgattca gtcttcataa cccccgaggt aatgctggaa
    15841 ttaagaaact gcatcgacct tgctcctttg cataacccac ataacatcag aggcatcaat
    15901 gcctgtcaga gaatactgcc tggggtaccg caagtggctg tctttgatac cgcttttcac
    15961 cagaagatgc cacccgtggc atacatctat ggaatccctt acgtacttta taaaagatat
    16021 ggcatcagac gctatggttt tcacggcaca tctcattttt acgtttccca acggacaagc
    16081 cagctgatga ataaatccat aaaggagctt aagatcatca cttgccattt ggggaacgga
    16141 gcaagcatgg cggcagtgga ccaaggcata tcagttgata ccactatggg attcacccct
    16201 ttagagggac tattgatggg aacgcgttcc ggggatctag acccagccat aatattgcat
    16261 attatggcga gggaggaatt gtctttacac gaagccaata cccttttgaa caagcatagt
    16321 ggaatacagg gaatatcggg catctccagc gatatgcggg atataattga ggaatccagc
    16381 aaaggaaatg ctggggcgaa attagctttt gacatctatt gctatcgtat ccgtaaatat
    16441 attggagctt atgcggcaac aatgggaggt ctggatgttt tggttttcac cgcaggagta
    16501 ggggaaaatg ctcctttggt gagaaaagcg agttgtgaaa acttggagtt cttaggagtg
    16561 aagatagata gccaaaaaaa tgaccagatg ataggaaaag agggaataat aagcactgat
    16621 gactcaagag ttaaagtcct ggtcatcccc acgaatgaag aattggtcat tgcacaggat
    16681 accgagagga tagtggaaga acaagcccgt cccagagaaa caagcagggg gttgtaaaaa
    16741 aggaatcgga ttgaacgaaa ttattttcca agagaatttg gcaccccaga taaatgtgat
    16801 taagcttaaa cttccccaca tcgtgaataa agctaagccc ggccaatttg ttatcttgcg
    16861 cataaatgaa aaaggggaaa ggataccgat gtcgattgct gacttaaaca aagaggatgg
    16921 aatcctgacc attgtttttc aagaagtggg caagactacc gcccaattgg gaactctaag
    16981 aaagggggat tttttggcgg atgtggtggg acctttgggg attcccaccc atattgagaa
    17041 attcggtcat tgtgtgtgca ttggtggagg aattggaata gcccctattc atccaattgc
    17101 cagagcattg aagcaagctg gaaatcaggt tacctcgatt atcggagcgc gcagtaaaaa
    17161 tcttttgatt atgcaagagg aggtgaatga agcaagtcac aaggtcttgc cttccacgga
    17221 tgatggaact tacggcagga aaggatttgt gactgacatt ctgactgagc ttctaatcca
    17281 caagaacaaa atagactaca taatggctat tgggccggtc ataatgatga aggcagtatg
    17341 taatatcacc aagccgtacg gcattaagac catggtcagc ctcaacccga taatggtcga
    17401 tggtacgggg atgtgcgggg cttgtcaagt gacagtggaa ggaatgacca agttcgcttg
    17461 tgtggatgga cctgatttcg atgggcataa agttgatttt gaagagctaa tcaaaagaca
    17521 acatatgtat cttaaacagg aaagaacagc catggagaga tataataaat aagagaagcc
    17581 caagatggca ataagcggac aaggtgcaaa gattcctcgg caaaagatga tggagcaaaa
    17641 gcccggggat agaataagaa acttcaacga ggttcctttt ggctatacct cggaaatggc
    17701 acaggtagaa gcttctcgct gcctgcaatg caagaaacct atctgcatat ccggctgtcc
    17761 ggtagagatc gatattccta ctttcttaaa actagtggcc cagggagatt ttatcggagc
    17821 agcaaagaag ctcaaggagg caaatagcct tccggctgtc tgcggaaggg tatgtcctca
    17881 agaggagcag tgcgagagga tctgtgttct aacaaaaaaa tttcagtccg tggggattgg
    17941 aaatctggaa agatttgctg cggattttga aagagagcaa ggggaagtgg aaattcccaa
    18001 gatagaaaag tcaaccggga aaagggttgc cattgttggc tccggcccgg ctgggttgac
    18061 cgtagcagga gacttaatca aaatgggtca tcaggtcacc atttttgaag ccctgcataa
    18121 agccggtggg gtcttagttt acggcattcc ggaattcaga cttcccaaag cgattgttca
    18181 agcggaagtt gattaccttc tgaaa
//
LOCUS       DASWLN010000076         3330 bp    DNA     linear   ENV 20-FEB-2024
DEFINITION  MAG TPA_asm: Candidatus Zixiibacteriota bacterium isolate
            SMAG_U2949 k141_162955, whole genome shotgun sequence.
ACCESSION   DASWLN010000076 DASWLN010000000
VERSION     DASWLN010000076.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37960594
            Sequence Read Archive: SRR10376091
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Candidatus Zixiibacteriota bacterium (soil metagenome)
  ORGANISM  Candidatus Zixiibacteriota bacterium
            Bacteria.
REFERENCE   1  (bases 1 to 3330)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 3330)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (26-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/12/2024 08:28:26
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 1,440
            CDSs (total)                      :: 1,416
            Genes (coding)                    :: 1,412
            CDSs (with protein)               :: 1,412
            Genes (RNA)                       :: 24
            rRNAs                             :: 1 (16S)
            partial rRNAs                     :: 1 (16S)
            tRNAs                             :: 21
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 4
            CDSs (without protein)            :: 4
            Pseudo Genes (ambiguous residues) :: 0 of 4
            Pseudo Genes (frameshifted)       :: 0 of 4
            Pseudo Genes (incomplete)         :: 3 of 4
            Pseudo Genes (internal stop)      :: 1 of 4
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..3330
                     /organism="Candidatus Zixiibacteriota bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_162955"
                     /isolate="SMAG_U2949"
                     /isolation_source="soil"
                     /db_xref="taxon:2053527"
                     /environmental_sample
                     /country="USA: Oregon"
                     /lat_lon="42.99 N 123.63 W"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            <1..239
                     /locus_tag="VGB16_02555"
     CDS             <1..239
                     /locus_tag="VGB16_02555"
                     /EC_number="5.4.99.18"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_018046960.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0034023 - 5-(carboxyamino)imidazole
                     ribonucleotide mutase activity [Evidence IEA];
                     GO_process: GO:0006189 - 'de novo' IMP biosynthetic
                     process [Evidence IEA]"
                     /codon_start=3
                     /transl_table=11
                     /product="AIR carboxylase family protein"
                     /protein_id="HEX9916594.1"
                     /translation="ASHTTLPVIGVPLVSKHLSGLDALLSMAQMPAGVPVATVALGEA
                     GAKNAAILAGEILSLADKNLKRKIERFKKELGKS"
     gene            511..1074
                     /locus_tag="VGB16_02560"
     CDS             511..1074
                     /locus_tag="VGB16_02560"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_004029594.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="LemA family protein"
                     /protein_id="HEX9916595.1"
                     /translation="MVVALLLLLIVIGVPLIIVIGVYNRLVVFRNRGDNAWHQIDVQL
                     QKRADLVPNLVETVKGYAAHEKQTFENVTKARAMYNQAEDIKGKAEATNMLTSALKTL
                     FAVAENYPELKANQNFLLLQEELSGIESKIAYARQFYNDTILDYNNSRQVFPSSIIAN
                     LFSFQPRDYFEIEEPAKREVPKVDFGK"
     gene            complement(1192..1959)
                     /locus_tag="VGB16_02565"
     CDS             complement(1192..1959)
                     /locus_tag="VGB16_02565"
                     /inference="COORDINATES: protein motif:HMM:NF012622.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="dockerin type I repeat-containing protein"
                     /protein_id="HEX9916596.1"
                     /translation="MRRALILIVLVFSLTLFFPRLGSAQVNEYYLVPVNLFIYPSGGT
                     VEVDIHIELFEEYGMIKAFFVPLYAEGTSNPVLDTVLTGGLMDAYPPAFHPPSLVDYF
                     SVTTVNPYGPLTEPLYFLAMNFGQAGITPPAKGLYCRMFYKVSGPGTLTFRTAVHSTG
                     GPVLMNDYYGSSVPINWPPEGEVGNFEVTAIQPGDANRDGHVTVSDVIYLINYLFKSG
                     PPSNPAIAGDANCDGQVSVSDVIYLINYLFKSGPPPC"
     gene            complement(2255..>3330)
                     /locus_tag="VGB16_02570"
     CDS             complement(2255..>3330)
                     /locus_tag="VGB16_02570"
                     /inference="COORDINATES: protein motif:HMM:NF012622.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=3
                     /transl_table=11
                     /product="dockerin type I repeat-containing protein"
                     /protein_id="HEX9916597.1"
                     /translation="GWNKKEAGWITPVGLTGDTHIVIRNIETTEDSSLYLLPINPSEG
                     EYFLLEYRNPTSAGKFDKIDSDFSVYFWPYLTFGGDSLDRGLLITHVHDSLGANYWRI
                     NSGTPTYAHYTVAVEDAGYNPDYDASHNPEGHVTDSSQWWYPYESRRAAPFSNDVTGQ
                     NIFSPSTYPNSDGYSGSSGITVRVDSIVNDRLYAYVYAPIPAFSLLSPSDSVFVPYKV
                     TFQWANPDPWGELEYDLYVSTSPNFNPDSTVIHSGLSLNSYNDSLAIGRYYWKVRAFN
                     PSIQKWSTQTRTFLSAKRGDANGDTRVTVSDVVYLINYLFKSGPPPIPEQIVGDANCD
                     GKATVSDVVYLINYLFKSGPPPC"
BASE COUNT          962 a          682 c          804 g          882 t
ORIGIN      
        1 ttgcctccca tacgactctg ccggtaattg gtgttcctct ggtaagtaag catctttccg
       61 ggttggatgc ccttttgtct atggctcaga tgccagctgg agtgccggtg gcaacagttg
      121 ctttgggtga agccggggca aagaatgcgg caattttggc aggggagatt ttaagtttgg
      181 cagataaaaa tttaaagaga aaaattgaaa gatttaaaaa agaattgggc aaaagttagt
      241 tcgtttggtc aattgatgtg tcattgcgag gagtcctgag ccgtgtcgaa ggacgacgaa
      301 gcaatctgac aaccataaag ttgtcatccc gaacgaagtg agggatctcc tcagggtgag
      361 atccttcgct tacgctcagg atgacgcttg cgcttcagat tgcttcgctt gcgctcgcaa
      421 tgacaaaagt aattcaattt ggtttaattt ctttgatagt atttggtata ttgatattaa
      481 aataaatcaa ctttttaaaa aggagaagat atggtagtag cgttgttgtt gcttttgata
      541 gtgattgggg ttccattgat aatcgtcatt ggagtataca atcgcctggt ggtctttcgc
      601 aatcggggag ataatgcctg gcaccagatt gatgtacaat tacagaagag agcagattta
      661 gtccccaact tagtggaaac ggtcaagggt tatgcggcgc acgagaaaca gaccttcgag
      721 aatgttacca aagccagggc gatgtataat caagctgaag atatcaaagg caaggcagaa
      781 gccacaaata tgctcacctc tgccttgaaa accctttttg cggtggcgga gaactatccg
      841 gaactgaagg ccaatcagaa ctttttgctt ttgcaggagg agctttctgg aattgaaagc
      901 aagatcgctt acgctcggca attctataac gatactattt tagattacaa caattcgcgt
      961 caggtttttc catccagcat catagcgaat ttatttagtt tccaacccag agattatttt
     1021 gaaattgaag agccggcaaa aagggaagtg cccaaggttg attttgggaa gtaggttaaa
     1081 acagtcttgc cctgcaccgt aatggtgcag ggttagagtt ttgggtttca agaaaaaaaa
     1141 caggggcagg attaattcct gcccctgtgg gattattaga atctaaattt gctaacacgg
     1201 cggcggtccg cttttaaaaa gatagttgat taaatagatt acatcgctta cagatacttg
     1261 accatcgcaa ttggcatctc cggctattgc aggatttgaa ggaggaccgc tcttaaaaag
     1321 atagttaatc aaataaatca catctgaaac agttacgtgt ccatcacgat tagcatctcc
     1381 cggctggatt gctgtcacct caaaattgcc cacctctcct tcagggggcc agttaatggg
     1441 aactgatgaa ccataataat cattcattaa cactggtcca ccagttgagt ggactgcagt
     1501 tctgaaggtt aaagttcctg gaccagaaac tttgtagaac atcctgcaat aaagcccttt
     1561 ggctggagga gtaatacctg cctgtccaaa attcatagcc aaaaagtata atggctcagt
     1621 gagtggtcca taagggttta cagttgtcac ggaaaaataa tcaaccaggc tgggcgggtg
     1681 aaaggctggg ggataggcat ccataaggcc accggttaac acagtatcca aaaccgggtt
     1741 agaagtcccc tccgcatata agggaacaaa aaatgccttg atcataccat actcttcaaa
     1801 aagctcaata tgaatgtcaa cctccacggt tccaccagag gggtagataa aaagatttac
     1861 cggaacgagg tagtattcat tgacctgtgc agacccgagc ctgggaaaga aaagcgtcag
     1921 ggaaaatacc aagacaatta atatcaaagc tcttctcatt tattatctcc tttgtaggga
     1981 cagaacattg ttctgtccat ctagctcatt tatcaggtgc tttagggcac ctgacctgca
     2041 aactctaaag gtaagatgtc gttgcacaga acccccttga ttagaggatg acatcgggag
     2101 gaataaaaat gtgaagtcgg ccatcttctt aagctaacct gccactatta aaaataggcc
     2161 aacatctgtt tttgtcaata caaaactggg ctatgatgtg agcagacctg gggtccctgc
     2221 tctggtcggt gaggttgcta tactttcggc ctccctaaca agggggaggg ccgcttttaa
     2281 aaagatagtt gatcaggtaa actacatctg aaacagttgc ttttccatca caattagcat
     2341 ctccaactat ctgttcaggt atcggcggcg gtccagattt gaaaagatag ttaatcagat
     2401 aaactacatc cgaaacagtt acacgtgtat caccatttgc atccccacgc ttggcggaaa
     2461 gaaaagttcg ggtttgggtt gaccattttt ggatagaagg attgaaagct cttactttcc
     2521 aataatatct tccaatcgca agactgtcat tgtagctgtt aagggagagt ccactgtgaa
     2581 tgactgtgga gtcaggatta aagttgggcg aagtgctgac atacaaatca tattcaagtt
     2641 ctccccaggg atcggggttt gcccactgga aagtcacttt gtatggaacg aaaacgctgt
     2701 ccgaaggcga aagcaaggaa aaagctggaa tgggagcgta aacataggca tacagtctat
     2761 cgttcacaat cgagtccact ctaacagtta ttccagaaga accggagtag ccgtcgctgt
     2821 tgggataagt ggaagggctg aatatgttct gcccggtcac gtcattggaa aagggagctg
     2881 cccttctgct ttcgtaggga taccaccatt gggaactatc ggtaacatgg ccttctggat
     2941 tgtgagaggc atcatagtcc ggattgtatc ccgcatcctc cactgcaacc gtataatggg
     3001 cataagtagg ggttccgctg tttatcctcc aataatttgc gcccagtgaa tcatgcacat
     3061 gagttatgag taaacctctg tcaagagaat ctccaccgaa ggtcagatag ggccagaaat
     3121 aaacactgaa gtcagaatca attttatcga atttgccggc agaggtcgga ttacggtatt
     3181 ccaggaggaa atactctccc tcgctcggat ttatgggaag aagatacagt gagctgtcct
     3241 cggtagtctc gatattgcgt atgacgatgt gggtgtcgcc ggttaagccc acgggagtaa
     3301 tccatccggc ttctttcttg ttccatccgc
//
LOCUS       DASWLN010000077         5241 bp    DNA     linear   ENV 20-FEB-2024
DEFINITION  MAG TPA_asm: Candidatus Zixiibacteriota bacterium isolate
            SMAG_U2949 k141_1177195, whole genome shotgun sequence.
ACCESSION   DASWLN010000077 DASWLN010000000
VERSION     DASWLN010000077.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37960594
            Sequence Read Archive: SRR10376091
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Candidatus Zixiibacteriota bacterium (soil metagenome)
  ORGANISM  Candidatus Zixiibacteriota bacterium
            Bacteria.
REFERENCE   1  (bases 1 to 5241)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 5241)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (26-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/12/2024 08:28:26
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 1,440
            CDSs (total)                      :: 1,416
            Genes (coding)                    :: 1,412
            CDSs (with protein)               :: 1,412
            Genes (RNA)                       :: 24
            rRNAs                             :: 1 (16S)
            partial rRNAs                     :: 1 (16S)
            tRNAs                             :: 21
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 4
            CDSs (without protein)            :: 4
            Pseudo Genes (ambiguous residues) :: 0 of 4
            Pseudo Genes (frameshifted)       :: 0 of 4
            Pseudo Genes (incomplete)         :: 3 of 4
            Pseudo Genes (internal stop)      :: 1 of 4
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..5241
                     /organism="Candidatus Zixiibacteriota bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_1177195"
                     /isolate="SMAG_U2949"
                     /isolation_source="soil"
                     /db_xref="taxon:2053527"
                     /environmental_sample
                     /country="USA: Oregon"
                     /lat_lon="42.99 N 123.63 W"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            2..304
                     /gene="cutA"
                     /locus_tag="VGB16_02575"
     CDS             2..304
                     /gene="cutA"
                     /locus_tag="VGB16_02575"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000388230.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_process: GO:0010038 - response to metal ion [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="divalent-cation tolerance protein CutA"
                     /protein_id="HEX9916598.1"
                     /translation="MFVTTNSTASAKTIGKKLVEQKLVACVNIVPKVKSIFRWKGKLE
                     KEDEVLLVLKTKRILFDKVVQQVKKIHPYEVPEIVGLPIICGNKSYLDWIEREVEG"
     gene            305..724
                     /locus_tag="VGB16_02580"
     CDS             305..724
                     /locus_tag="VGB16_02580"
                     /inference="COORDINATES: protein motif:HMM:NF015444.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="PPC domain-containing DNA-binding protein"
                     /protein_id="HEX9916599.1"
                     /translation="MFFQKIKDGYVIRLEKGEEIINSLADFAGKYKIEGGFIYGIGTV
                     NNLTLGYYQAGKKNYLKKNFPDDFELVSLMGNISLLKGNPMVHTHVVVSDVNFNTLAG
                     HLFSGTISVTGEFVILATKDAIERAFDNQTGLNLLQL"
     gene            721..1506
                     /locus_tag="VGB16_02585"
     CDS             721..1506
                     /locus_tag="VGB16_02585"
                     /inference="COORDINATES: protein motif:HMM:NF014003.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="SAM-dependent chlorinase/fluorinase"
                     /protein_id="HEX9916600.1"
                     /translation="MTKFVTLTTDFGLNDSYVGQVKGVILKINPNVNLIDITHQIPAW
                     DVLRGAFVLNSFYKYFPKGTIHLVVVDPTVGSKRKRLLIQTRDYVFVGPDNGIFSFIY
                     ENEEIREILEIRNNKFFLNQESSTFHARDIFAPVVGYLSWGVEISQFGPKVKDCVKLN
                     LPLPEIKKDSIKGEIIYIDRFGNLISNISREHLNGFEKTKVSLTIGNRKIEKLSRSYS
                     DAKPKQILALVNSSNLLEISVRQNRADEVLNVKIGDKLGINLK"
     gene            1503..1982
                     /locus_tag="VGB16_02590"
     CDS             1503..1982
                     /locus_tag="VGB16_02590"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_015721294.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="cytidine/deoxycytidylate deaminase family
                     protein"
                     /protein_id="HEX9916601.1"
                     /translation="MKKNVSRPSWDEYFMNIAQLAATRSTCLRRQVGAAITKDKKILA
                     TGYNGAPAGLKHCLDIGCLREEMGIPSGERHELCRATHAEQNAIVQAATHGVSIKDSV
                     LYSTTHPCILCSKLLINAGIKKVVIEESYPDELSQKMLKEARVEILVLKNKKRRRRK"
     gene            1979..2428
                     /gene="nrdR"
                     /locus_tag="VGB16_02595"
     CDS             1979..2428
                     /gene="nrdR"
                     /locus_tag="VGB16_02595"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_005999702.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0140110 - transcription regulator activity
                     [Evidence IEA];
                     GO_process: GO:0006355 - regulation of DNA-templated
                     transcription [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="transcriptional regulator NrdR"
                     /protein_id="HEX9916602.1"
                     /translation="MKCPFCGCAEDKVIDSRTSQDGRSVRRRRECLSCNKRFTTYEYI
                     EGVSLTVAKNDGRREPFDRQKLVRGIELACNKRPISSKRILALVDEIEEYLQNLSKKE
                     VTSKEVGEVVMSKLKELDEVAYVRFASVYRQFKDKGEFMQELKNLLG"
     gene            2436..3164
                     /gene="tatC"
                     /locus_tag="VGB16_02600"
     CDS             2436..3164
                     /gene="tatC"
                     /locus_tag="VGB16_02600"
                     /inference="COORDINATES: protein motif:HMM:TIGR00945.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0005886 - plasma membrane [Evidence IEA];
                     GO_process: GO:0015628 - protein secretion by the type II
                     secretion system [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="twin-arginine translocase subunit TatC"
                     /protein_id="HEX9916603.1"
                     /translation="MASVGREMSFLDHLEELRKRIIRSLIAVVIFSVAAYYFSSKIID
                     FVTKPLPKVYFMSPTEGFMIRIKIAIIVGVIVSAPVILYQLWRFIAPGLFKREIRTVG
                     PILVFSTFFFFAGGSLCFFLVIPTAVKFLLAFGTEKLQPLMKISDYISFVGFMVLAFG
                     AAFELPIVSYFLAKMGILGPQIMIKGRRYAVVGILILAGVLTPSPDVFSQLMLAVPLY
                     FLYEVSIIIVKVVHKNRLKKGMEQ"
     gene            3161..5182
                     /locus_tag="VGB16_02605"
     CDS             3161..5182
                     /locus_tag="VGB16_02605"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEX9916604.1"
                     /translation="MKRVLILGSIIFFGLSSAFAGDLVSKREEGLKEALKGLKMTRED
                     LSFRDDYTPKDTFRLKIIDDLMKSPLLSLDYSDSLALLLSSSKNFLDRLVRTEYQTKG
                     DGVTVSLSDSSLILGDLETGGSLQEEIEYLLEKNGQEISSDERGQIKEQIGRLPKEIG
                     ECLRIALSSLRRADEKLDGVFSVLNQDEIEQLENYVSTIILEDVEDQNKTPEELDLEA
                     KIEEKMAEDLIPLASRIDVPKINRIGAELADRMWYVKERLKQFTSKQENKGLLEKTYE
                     DSNILFYVDTPEGEIVIGGLGRNLYVGKKYLTIDLGGDDQYFIPELEEENEANSSVII
                     DLEGNDLYKVQRDYCFGSGFFGFGILFDCQGDDQYLANSFSLGAGLFGVGILVDEEGN
                     DNYVGDTYTQGAGGFGIGILQDQAGNDFYSGCLFAQGFGFVKGLGALIDMSGNDNYFA
                     GGKYKDIIRYQDHFISLSQGFAYGIRPILSGGIGVLVDLSGNDTYASDIFGQGSSYWW
                     GLGSLVDFSGNDNYISYQYAQGAATHMTLGCLVDMKGNDNYISHGVSQGCGHDYASGI
                     LEDREGNDNYITFGLSQGAGNANGIGIIIDHKGNDKYVVGQKNDTQGYGDGRREFGSI
                     GIFLDMSGKDEYIGGYGKDSTWWNTPSFWGIGIDGEFQKELYDKNVENK"
BASE COUNT         1594 a          873 c         1335 g         1439 t
ORIGIN      
        1 aatctttgtc accaccaatt caaccgcttc agccaaaaca attggaaaaa agttggttga
       61 gcaaaaattg gtggcttgtg ttaatatagt ccccaaagtg aaatcgattt ttcgctggaa
      121 gggaaaatta gaaaaagagg atgaggtttt gcttgtgctc aagactaaaa gaattctatt
      181 tgacaaagtc gtccaacaag taaaaaagat acatccttac gaagttccag agatagtcgg
      241 tttgcctata atttgtggaa ataaaagtta tcttgactgg atagaaaggg aagtggaggg
      301 atagatgttt tttcagaaga ttaaagatgg ttacgtcata agattggaaa aaggggagga
      361 gataatcaat tccctggctg attttgcagg taagtataaa atcgaaggcg gatttattta
      421 tggcataggc acggtgaata atttgacttt gggatattac caagcgggaa agaaaaatta
      481 tctcaagaag aattttccag atgattttga attagtcagt ttgatgggaa atatctcgct
      541 tttaaaaggg aatccgatgg tgcatacaca tgtggtagtc tccgatgtga atttcaatac
      601 cttagctggg catctttttt caggcacaat ttccgtgacc ggtgaatttg tcattcttgc
      661 aaccaaagat gcaattgaaa gagcgtttga taaccagacc ggactcaatc tcttacagct
      721 atgacaaagt ttgtgacctt gaccactgat tttggtttga atgatagcta cgtaggtcaa
      781 gttaaaggtg taatcttaaa gataaatccg aatgtcaact tgatagatat aactcaccaa
      841 ataccagctt gggatgtact aaggggcgca tttgtcttaa atagttttta caaatatttt
      901 cctaaaggga caatccactt ggtagtggtt gacccgacag tgggtagcaa aagaaaaagg
      961 ttgttaatac agaccagaga ttatgttttt gtggggccag acaatgggat tttcagcttc
     1021 atttatgaaa atgaagaaat aagggagatt ttggaaatca ggaataataa gttcttttta
     1081 aaccaagaat cgtccacttt ccacgccaga gacatcttcg cgccggttgt gggttatttg
     1141 tcctggggag tggaaatcag tcagttcggc ccaaaagtaa aagattgtgt taaacttaat
     1201 cttcctctgc cagaaattaa aaaagatagc atcaaaggag aaatcattta tattgatagg
     1261 ttcggaaacc tgataagtaa catttcaagg gaacatctga acggatttga gaaaacaaaa
     1321 gtcagcctaa ctattggcaa ccgaaagatt gaaaagctga gcagatctta ttctgatgcc
     1381 aagccgaagc aaatcctggc tttggtgaat agttcaaatc tcttggaaat atcagttcgc
     1441 cagaatagag cagatgaggt tttaaatgtg aaaatcggag ataaactggg gattaatctc
     1501 aaatgaaaaa aaatgtatcc cgaccttcct gggatgaata ttttatgaat attgcccagt
     1561 tagcggcaac acgttccacc tgtttgagaa ggcaggtggg agcggcaatc accaaagata
     1621 aaaagatttt agcaaccgga tataatggcg ctccagctgg attgaaacac tgcctggaca
     1681 ttggttgcct aagagaagag atgggaatac cttcaggcga gcggcacgag ctttgtcgag
     1741 ccactcatgc ggagcagaac gccattgttc aggcagccac gcatggggtt agcataaaag
     1801 attcggtgct atattctact acccatccat gcatactttg tagcaaacta ctcatcaatg
     1861 cgggtataaa gaaagtggtc atagaagaaa gctatccgga tgagctttcg cagaagatgc
     1921 tgaaggaggc cagagtggag attttggttt tgaagaacaa gaaaagacgg agaagaaaat
     1981 gaaatgtcct ttttgtggtt gcgctgaaga taaggtgata gattcccgca cctcccagga
     2041 tggcaggtca gtgcgtcgga ggagggaatg tctatcctgc aacaaacgtt ttaccaccta
     2101 cgagtatatc gaaggcgttt ccttgaccgt ggctaaaaat gacggaagaa gagagccatt
     2161 cgatagacag aagctggttc gggggataga gctggcttgc aataagcggc cgatcagctc
     2221 caagaggata cttgctttgg tggatgagat cgaggaatat ctgcaaaacc tctccaagaa
     2281 agaggtaacc agcaaggagg ttggtgaggt agtgatgtct aagctcaaag agctggatga
     2341 agtggcttat gtcaggttcg cttcggtcta tcgtcagttc aaggataagg gggagttcat
     2401 gcaggagttg aagaaccttt tggggtgagg ggcaaatggc tagtgtcggc agggagatgt
     2461 cgtttttaga ccatctggag gaactgcgca agagaatcat acgcagtttg attgcggtag
     2521 tgattttctc cgtagcagcc tactactttt cttccaagat aatcgatttc gtcactaaac
     2581 ccttgcctaa agtttatttc atgtccccga ccgaagggtt catgataagg ataaagatcg
     2641 ccatcatcgt gggggtgatt gtcagtgccc cggtaatcct ctatcagctt tggaggttta
     2701 ttgctcccgg tcttttcaaa agggagataa ggacagtcgg tccgattctt gttttctcca
     2761 cctttttctt ttttgccggt ggaagcttgt gtttcttttt ggtcattccg acagcagtga
     2821 agttcctttt agcttttgga acggagaaac ttcagcctct gatgaaaatc agcgactata
     2881 tcagctttgt agggtttatg gttttagctt tcggtgcggc tttcgagctg cccattgtct
     2941 cctatttctt ggccaaaatg ggaattctgg gaccacagat tatgattaaa ggcagaagat
     3001 atgctgtggt ggggattctg atactagcgg gagtcttgac cccttcccca gacgttttct
     3061 cccaattgat gcttgcagtt ccactctact tcctgtacga ggttagcata atcatcgtca
     3121 aggtggttca taagaacaga cttaagaaag gaatggagca gtgaaaagag ttttaatttt
     3181 aggttcaatt atatttttcg gtttgagctc ggcttttgct ggcgatttag tctccaaaag
     3241 agaagagggt ctgaaagagg cattaaaagg actgaagatg acaagagagg atttgagctt
     3301 cagagatgac tacacgccaa aggacacctt tcgattgaaa atcatagatg atttgatgaa
     3361 atcaccgctt ttgagcttag actattcaga cagtctggca ttgcttttat cctcctccaa
     3421 aaacttcctc gacagattgg tgagaacgga ataccagaca aaaggagatg gagttacagt
     3481 ttccctatcg gattcgtccc ttatcctggg agatttagaa acagggggga gtttacaaga
     3541 agaaatagaa tacttattgg aaaaaaatgg tcaagagata agttccgatg aaagagggca
     3601 gataaaagaa caaattggga gattgcctaa agaaatagga gaatgtttga gaatcgctct
     3661 ttccagcctg cgccgggcag atgagaaatt ggatggtgta ttctcggtgc tgaatcaaga
     3721 tgaaatagag cagttggaaa actacgtcag cactattatc ctggaagacg tggaggacca
     3781 gaataagact ccggaggagc tggatttaga ggctaaaata gaggagaaga tggcagagga
     3841 tttgattcct cttgcaagca gaatagatgt gccaaagata aacagaatcg gcgcggaatt
     3901 ggcagacagg atgtggtatg tcaaggagag attaaaacag ttcacaagca aacaggagaa
     3961 taaggggtta ttggagaaaa cctatgaaga ttctaatatc cttttttacg tggacactcc
     4021 tgaaggagag atagtgattg ggggattagg gagaaatctc tatgttggta agaaatacct
     4081 aaccattgat ttgggagggg atgaccaata tttcattccg gagcttgagg aggagaacga
     4141 agcgaactct tcggtgataa ttgatttgga gggaaatgat ttatataaag tccagaggga
     4201 ttattgtttt ggcagtggct tttttggatt tgggatactt ttcgattgtc aaggggatga
     4261 ccaatatctg gcaaacagtt tctctttagg tgcggggctt tttggagtag ggattttagt
     4321 ggatgaagaa ggaaacgata actatgtagg agatacctat actcagggag ctggcggatt
     4381 cggaattgga atcctgcaag accaagccgg aaatgacttc tactcaggtt gtcttttcgc
     4441 acaggggttt ggttttgtca agggactcgg agcattgata gatatgtccg gaaatgataa
     4501 ctactttgct ggaggtaaat acaaagatat aatccgctat caagaccact tcataagtct
     4561 ttctcaagga tttgcctacg gcatcaggcc aatactgtca ggtggcatag gagtcttggt
     4621 tgacctttca ggtaacgata cctacgcctc agatattttc ggtcaaggtt caagctattg
     4681 gtggggatta gggagcttgg ttgatttttc cggcaatgac aattacatct cctatcaata
     4741 tgctcaagga gcggcaactc atatgacctt aggatgttta gttgatatga aaggtaatga
     4801 taattacatc tcgcatgggg tgtcgcaagg ttgcggccat gattatgcca gtggaattct
     4861 agaggataga gaagggaatg acaattacat aacttttggg ctttcgcaag gggctggaaa
     4921 tgccaatgga ataggaatta ttatagacca taaaggtaat gataaatatg tggttggaca
     4981 gaaaaatgat acccagggat atggtgatgg aagaagggag ttcgggtcaa tcgggatatt
     5041 tttggatatg tcaggtaagg atgaatatat tggaggatac ggcaaagaca gcacctggtg
     5101 gaacacccct tccttttggg gaataggaat agacggagaa tttcagaagg agttgtatga
     5161 caagaatgtt gaaaataagt agcataattt ttttatttgg tttttcgctt gtttacgccc
     5221 aagggccgga aaatactctt t
//
LOCUS       DASWLN010000078         8205 bp    DNA     linear   ENV 20-FEB-2024
DEFINITION  MAG TPA_asm: Candidatus Zixiibacteriota bacterium isolate
            SMAG_U2949 k141_74745, whole genome shotgun sequence.
ACCESSION   DASWLN010000078 DASWLN010000000
VERSION     DASWLN010000078.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37960594
            Sequence Read Archive: SRR10376091
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Candidatus Zixiibacteriota bacterium (soil metagenome)
  ORGANISM  Candidatus Zixiibacteriota bacterium
            Bacteria.
REFERENCE   1  (bases 1 to 8205)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 8205)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (26-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/12/2024 08:28:26
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 1,440
            CDSs (total)                      :: 1,416
            Genes (coding)                    :: 1,412
            CDSs (with protein)               :: 1,412
            Genes (RNA)                       :: 24
            rRNAs                             :: 1 (16S)
            partial rRNAs                     :: 1 (16S)
            tRNAs                             :: 21
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 4
            CDSs (without protein)            :: 4
            Pseudo Genes (ambiguous residues) :: 0 of 4
            Pseudo Genes (frameshifted)       :: 0 of 4
            Pseudo Genes (incomplete)         :: 3 of 4
            Pseudo Genes (internal stop)      :: 1 of 4
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..8205
                     /organism="Candidatus Zixiibacteriota bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_74745"
                     /isolate="SMAG_U2949"
                     /isolation_source="soil"
                     /db_xref="taxon:2053527"
                     /environmental_sample
                     /country="USA: Oregon"
                     /lat_lon="42.99 N 123.63 W"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            <1..274
                     /locus_tag="VGB16_02610"
     CDS             <1..274
                     /locus_tag="VGB16_02610"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=2
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEX9916605.1"
                     /translation="IITLVELGSVSEQNQQLAERVALWTLVNMQDREGYFYFQRKSLL
                     TNRIAYMRWSQAWMLKALVTLLTAQRALNDKTFVDQVATRHSLFTS"
     gene            369..1154
                     /locus_tag="VGB16_02615"
     CDS             369..1154
                     /locus_tag="VGB16_02615"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003443704.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="WecB/TagA/CpsF family glycosyltransferase"
                     /protein_id="HEX9916606.1"
                     /translation="MNRIMLFGCPIDTLTFDDTLRKIEDSIQKRKLIRHCVINAAKIV
                     KMKSDRKLREIVVSSDMISPDGQSIVWASRFLKKPLPERVTGIDLMQKLLKLASEKGY
                     KVFLLGAKEEVICELKEKLKRELPNLNLVGCRNGYFSPAEEEGIVKEINEKKSDILFV
                     GMSSPKKEYWMSKYQSDLKVPFCMGVGGSFDILVGKTKRAPRWMQRVGLEWFYRFLQE
                     PTRMWKRYLVTNTLFIFYLLKEKFLDLPDTRSDNEKTSEHLFR"
     gene            1280..2056
                     /locus_tag="VGB16_02620"
     CDS             1280..2056
                     /locus_tag="VGB16_02620"
                     /inference="COORDINATES: protein motif:HMM:NF024214.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016779 - nucleotidyltransferase activity
                     [Evidence IEA];
                     GO_process: GO:0009058 - biosynthetic process [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="sugar phosphate nucleotidyltransferase"
                     /protein_id="HEX9916607.1"
                     /translation="METILNRDVMQRVILAGGSATRMRPMSLDIPKSMIKIEGKPFLE
                     YQLELLRKNQIMEVALCIGHLGWQIRRYFGNGNKFGVNIKYSDEKDDLLGTGGALKKA
                     ENLLQDEFLVMYGDSYLSIDFGAVASYFKQFRKKSLVVVYENQNKFDKSNIELEENLV
                     KAYDKNNQGMSFIDAGVSVLKKEVLKLVPSKCDFPLEKLYQMLIKEKEMLAYQTAQRF
                     YEIGSFEGLEEFKNLVQVFEKSKSGKISVGASPTGVSTFR"
     gene            2526..4091
                     /locus_tag="VGB16_02625"
     CDS             2526..4091
                     /locus_tag="VGB16_02625"
                     /inference="COORDINATES: protein motif:HMM:NF025418.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="capsule assembly Wzi family protein"
                     /protein_id="HEX9916608.1"
                     /translation="MTRRKTLRKTKVGLIVSFLIFVLNTTVANSSQTISLNDEAYGWV
                     YEQIDYLYSRGFLDGLHYGTKPYTAEEVAKAVRVAQDKIDNQEIASNWVDEYVVKRLE
                     EEFAEDFQSEETKANCEINFSEKLTGESKEKPTYREKLRIGAGLKPNPHFSAYTRYII
                     DEALAKDSSYTGKVWKGFAGDASQSYISFDLPNVSFYFGRDRLSWGQGRLSSLILSET
                     AFPVDMLRLEGEWGILKFSSFTGVLSPMEIEDSTGTTHINRYLSGHRLSFAPCSWLQI
                     GLSETVLYGGENRNLELYYAIPFIWYHGVQLNEGRDDNTFLGLDLNLRPLHNLVFYGE
                     LLIDDIQIEKKQQGDKEPDELGFLGGVNLTNILLPGIEVNLEYQKITNWTYNQAKPYN
                     RYLHKNKTIGSSLGTDSDMLGASLSGWIVEGLRFGLSYSFGRQGQGRIDAPWDWPWLG
                     TEGEYKEKFPTGVVEKKKISSAYLEYDHHNRLRLRLFGKYADICNEGNIENNKESYKE
                     GGVLFFCMLGGLR"
     gene            4103..5521
                     /locus_tag="VGB16_02630"
     CDS             4103..5521
                     /locus_tag="VGB16_02630"
                     /EC_number="2.7.8.-"
                     /inference="COORDINATES: protein motif:HMM:TIGR03025.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0005886 - plasma membrane [Evidence IEA];
                     GO_function: GO:0016740 - transferase activity [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="sugar transferase"
                     /protein_id="HEX9916609.1"
                     /translation="MGIFLKDKFTVFKRLHLIADLALTALALNLAVLVDCLVKGEYKV
                     ISFGSEHLIFLVLPIWLIFLLSSKESYEYRLKSFVQIAKSISLVVLKSMGILLVSLFV
                     IKSRINSRLLLGSLVVFDLVLLLGIRGAIAITLSYMCKRGYNLKTILIIGSGKIATDF
                     VQEVKRHPRWGYSLLGIIDWEEERKGKDILGIPIIGTLNDLPDVVKSNHVDYVVYAVG
                     RKYLGLVEKSMLVCEEMGVTACILADFFPLKFSKKRVTQFSGKPMIFFTTTPDEYWSM
                     LIKYLFDRLLALVGLVLVSPIMLFTSILIKLTTKGPILFKQQRCGLNGKRFTLYKFRT
                     MLENAEELRASLSDKNEMDGPVFKMTNDPRLTKIGNRLRKYSIDELPQLFNVLLGDMS
                     LVGPRPPLPSEVSQYDHWQRRKLSMKPGITCLWQVNGRNNVNFQEWMKLDLQYIDNWS
                     LWLDAKILLKTIPAVTKGTGAK"
     gene            5518..6345
                     /locus_tag="VGB16_02635"
     CDS             5518..6345
                     /locus_tag="VGB16_02635"
                     /inference="COORDINATES: protein motif:HMM:NF025104.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEX9916610.1"
                     /translation="MNGSRFFKRAWDRKKGSWFIKFLLFVSYFFALSLSLVISYLCVR
                     KYDWIKEPSLVISWVVVVSIFLIVFLLQGFYQQRMAASKKREALLIIKGVMVGSILLA
                     ILVFVSNGKLVFNQREGILFFFLSSLVLLALFRSILFFHLRRHLLKKGIGVTNVLIVG
                     ANPEAKEFSKSLELIEPFKYRIAGLVEKEEKIRTDSYNGFKILGNLKDICKLIDQYKI
                     SEIYITSESLLPIEVLMVVEECRNLIGRIYIAAEMFEVLSERINLGKLKGVQLLKIR"
     gene            6451..7944
                     /locus_tag="VGB16_02640"
     CDS             6451..7944
                     /locus_tag="VGB16_02640"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_013008175.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEX9916611.1"
                     /translation="MKLQRRCPLIIFMVSLTLFLFNLAWPYDTPGTSVRATGMGGSFT
                     ALANDPSAVFYNPAGLVQLKGVQFYLMYDRKTVYGVNSAESPYLGAGAISFPLSRDIV
                     IALAGYQDGSSADPTGIVTKNIGLLSFSSWVTEELSFGLNLKGLYNSNYGKKKGFDFD
                     LGLLYRPHPWLGIGIMGENLARTDMVPDISSGVVSGYQTRQVKLGLAYHYEGKNYETC
                     LALDNTIKDANQPIQKSYNLTSIGMEQWILTDNKLNLALRGGYSFGKDYELNYSQPAF
                     GGSIRYKAQDYTIQLDYSWSKYPYESEEDLAGDHRVGLVISLEKGVKRGEKLLLKEEM
                     KEEKKEEAIVEKKEAPITLPSAPIEEKKESYPAKPEPEFLPEEKDSTKGSPEILTYSL
                     NSEIEFLSVGKNRSVMLLLKPEISQEIERWKLYIWSALPTGWSKEEVEKSLVKTFEGW
                     GNPSFGVMWDCRYNDVKVKSGTYYYALALKDNQGAKWYSQIKSFKLD"
     gene            7973..>8205
                     /locus_tag="VGB16_02645"
     CDS             7973..>8205
                     /locus_tag="VGB16_02645"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEX9916612.1"
                     /translation="MKKRTAERCGIGIPPRHSSSPQSGEVFWRRRINLPSPSPRFGPP
                     RRAIGETGEAGLFSKEGKKEVSPFEKGGFDGSI"
BASE COUNT         2452 a         1534 c         2061 g         2158 t
ORIGIN      
        1 gatcattacc ttggttgaac ttggctcggt tagcgaacaa aatcagcaat tagcagagag
       61 ggtggcactg tggacactgg taaatatgca ggatagagaa ggatattttt attttcagag
      121 gaaaagttta ttaactaaca ggatcgctta tatgcgctgg tcgcaagcct ggatgcttaa
      181 agctttggtg actctgttga ctgcccagag ggcacttaat gataagactt ttgtagatca
      241 ggttgccacc cgacattctt tgtttacttc atgacttgcg cggcagagcc ttcatggctc
      301 tgcggttgga cgaccgccgg tctgagttga aggcataaaa tttccgctac gtaaaaactg
      361 gggaagatat gaatagaata atgctttttg gatgtcctat agacactcta acctttgacg
      421 atacgttgag aaaaatagag gattccatcc aaaaaagaaa gctaatccgg cactgtgtca
      481 taaacgccgc aaaaatcgtc aaaatgaaaa gtgataggaa acttcgagaa atagtggttt
      541 cctcagacat gattagcccg gacggccaat ccatcgtatg ggcttcccgt tttttgaaaa
      601 agccacttcc cgagagagta accggcatcg atttgatgca gaaactcctc aagctcgctt
      661 ctgaaaaagg ctacaaagtt ttccttttgg gagccaagga agaagtaata tgtgaactta
      721 aagaaaaact aaaaagagag cttcccaatt taaacctggt gggatgcaga aatggttatt
      781 tttcacctgc cgaagaggaa ggaatagtga aggagataaa cgaaaagaag tcggacattc
      841 tcttcgtggg aatgagtagc cccaaaaagg agtattggat gagcaaatac caaagcgatt
      901 tgaaggttcc cttctgtatg ggagtaggag gaagctttga cattttggtg ggcaaaacca
      961 aaagagcccc ccgatggatg caaagagttg ggttggagtg gttttacagg ttcttacaag
     1021 agccaaccag aatgtggaaa agatatttgg taaccaacac ccttttcata ttctatcttc
     1081 tgaaagaaaa gttccttgat ctgccagata ctcgatcgga taatgagaag acgtcagagc
     1141 atctgttccg ataagcgctg agattacggc ggaggattcg tggaggatta tcgtaggaaa
     1201 gctatgctaa gaattaagag tcaatatggt tgggaaggga taacggatcg ctatgaagaa
     1261 ctctttgcaa gtatgttgaa tggaaacgat tttaaacaga gacgttatgc agcgcgtgat
     1321 attagcaggc ggatcggcaa ccaggatgag gccaatgagt ttggatatcc cgaaatcgat
     1381 gatcaaaatc gagggaaagc cgttcctaga ataccagctt gaattattga ggaaaaatca
     1441 aatcatggaa gtggccttat gcatcggtca tttaggatgg cagataagaa ggtacttcgg
     1501 aaacggcaat aagtttgggg taaatataaa atacagtgat gaaaaggatg atcttttggg
     1561 taccggagga gctttgaaaa aggcggaaaa tttactgcaa gatgaattct tggtgatgta
     1621 cggcgattca tatctgagca tcgattttgg ggcagtggct tcttacttta aacaattccg
     1681 aaaaaaatca ctggtggtgg tttatgaaaa ccaaaacaag ttcgataaga gtaatataga
     1741 actcgaagaa aacctggtta aggcgtatga caaaaacaac cagggaatgt cgttcatcga
     1801 tgccggagtt tcggttctca aaaaggaagt cctaaagctt gtcccctcca agtgcgactt
     1861 cccgctggag aaactctatc agatgttaat caaagaaaaa gagatgttag cttaccagac
     1921 agcccagcgt ttttatgaaa taggttcatt tgagggtttg gaggagttta aaaatttagt
     1981 gcaggtcttt gaaaagtcaa aatcgggaaa gatttctgta ggggcaagcc cgactggagt
     2041 gtctaccttt aggtagacct ttagttcggc taaagccgaa cactccaaag tacgttcaag
     2101 tcggatgcct gcccggccag gggcaggaat accgatataa tgcccctcac cttaatcctc
     2161 tccccaatgg ggagaggaaa gagaggaaga tgccgtgcgg gtcggatgaa agaacctaac
     2221 cccctgtatc ccccttgtca gggggacaga aaatgtttct cccttgataa ggggaggtca
     2281 ggaggggttc aagaagaggc catgcgggtc gcatgaatgc gacccctaca gatatttttg
     2341 gccttgtttg tcattcctgc gaaagcagga atccagaaag aaatgatgac aaaagctggg
     2401 ttttcaacat caagtgcgga atgacaagta tcaggtctac aggatctata ggaagtagcc
     2461 ttgacacggc accatagaga ttcttcgccc gtagggctca gagataacaa aaaatggagt
     2521 ttgcaatgac aagaagaaaa acacttagga aaacaaaggt tgggttaatc gtttcatttc
     2581 taatatttgt tctgaatacc acggtagcta attcgagcca aaccatttcc ttaaacgatg
     2641 aggcttatgg ttgggtctat gaacagatag attatcttta ttctcgcggt ttcttagatg
     2701 gcttgcacta tggcaccaaa ccttacactg cggaagaagt ggcgaaagcg gtgagggtgg
     2761 ctcaggataa aatagataat caggagatag caagtaactg ggtggatgaa tatgtggtta
     2821 agagactgga agaggagttc gcggaagatt tccaatcaga agagacgaaa gctaactgtg
     2881 agattaattt ctctgaaaaa ttgacaggtg agtctaaaga aaaaccgact taccgggaaa
     2941 aattgcgcat aggagctggc ctgaaaccaa atccgcattt ttcagcttac acccgctata
     3001 tcatagatga agctctggcc aaggattcaa gttacaccgg caaggtctgg aaagggtttg
     3061 ccggagatgc cagtcagtcc tacatctctt ttgacctgcc caacgtcagt ttctactttg
     3121 gtagggacag actgtcctgg ggccaaggca ggctttcttc cctgatatta tcggaaactg
     3181 catttccggt ggacatgttg cgcttagagg gtgagtgggg aatattgaaa ttcagctcct
     3241 ttacaggagt attaagtcct atggaaatag aggatagtac tggaacaacc catataaaca
     3301 ggtatctctc tggtcatcgc ttaagctttg ccccctgttc ttggttacaa attggccttt
     3361 ccgaaacagt gctttatggc ggggagaaca ggaaccttga gctttattat gctattccat
     3421 ttatctggta tcacggggtt caactaaatg aaggaaggga tgataatact ttcttgggtt
     3481 tggatctcaa tctcaggcca ctgcacaatc tggtcttcta cggagaattg ttgattgacg
     3541 atatccagat agaaaagaaa caacaaggcg ataaagagcc ggatgagctg ggttttttgg
     3601 gaggtgtaaa cctaaccaat atcctcttgc cgggaataga agttaatctc gaatatcaaa
     3661 agataaccaa ctggacttac aatcaagcca agccgtacaa tcgctatttg cataagaaca
     3721 aaaccatcgg cagcagtttg ggcacagata gcgatatgct cggggcttca ctctccggtt
     3781 ggatagtgga gggtttgcgt tttggtctgt cttattcttt tggccgtcaa ggacaaggga
     3841 gaatagatgc gccttgggat tggccctggt taggaactga aggggaatac aaggagaaat
     3901 ttccaactgg cgtagtcgag aaaaagaaaa tcagttcagc ttacttggaa tacgatcacc
     3961 acaatcgctt gagattaaga ttatttggaa aatatgccga tatttgcaat gagggtaaca
     4021 tagagaacaa caaggagagc tataaagagg gaggagtcct tttcttctgt atgctcgggg
     4081 gtttgaggta ggagggaaaa gtatgggaat atttctcaaa gataagttca cggttttcaa
     4141 gaggcttcat ctcatagccg atttagcatt gaccgcattg gctttaaacc tcgctgtctt
     4201 ggttgattgt ctggtcaaag gcgaatacaa agtcatctcc ttcggctcgg agcatctcat
     4261 atttttggtc ttgcccatct ggctaatctt tcttctttcc tccaaggaat cctatgaata
     4321 cagattgaag tcttttgttc aaatcgcgaa gagcattagc ttggtggtgc tgaaaagcat
     4381 ggggattctt ttggtttcgc ttttcgtcat caagtcgagg attaatagcc gtctgctctt
     4441 aggaagtctg gtggtgttcg atttggtgtt acttttgggc atacgtggag cgatagccat
     4501 cacgttgagc tatatgtgca aacgaggata caacctcaaa actatcctga taataggaag
     4561 cggaaagata gccaccgact tcgtccagga ggtgaagagg catccgcggt ggggatatag
     4621 ccttctcgga ataatagatt gggaggaaga gcggaagggt aaagatattc tgggtattcc
     4681 cataatcgga accctaaatg acttgcctga cgtggtcaag agtaatcatg tggattatgt
     4741 ggtctatgca gttggaagga aatatttggg tttggttgag aagagcatgc ttgtctgcga
     4801 agagatgggg gtgactgctt gcatcttggc tgatttcttc ccgctcaaat tctccaaaaa
     4861 gagagttact caattctcag gtaagccgat gatatttttt actaccaccc cggatgaata
     4921 ctggagcatg ctcatcaaat accttttcga tcgcttgctc gccttagtag gactggtatt
     4981 ggtatctcct atcatgctat tcacatctat tctgataaaa cttacaacca aaggcccaat
     5041 acttttcaag caacagagat gcggcttaaa cgggaagaga tttactctct ataaattcag
     5101 aaccatgctc gaaaacgccg aggagttgag agcctcactc tcggataaga acgagatgga
     5161 tggaccagtt tttaaaatga caaacgatcc tcgtcttacc aaaatcggaa atcgtttaag
     5221 aaagtatagc atcgatgaat taccccaatt gttcaatgtc cttttggggg acatgtcatt
     5281 ggtgggacct cgaccccctt taccctccga ggtgagtcaa tatgaccact ggcagaggag
     5341 gaaactttca atgaaaccgg gaattacctg cttgtggcaa gtaaatggcc gaaataatgt
     5401 aaactttcag gaatggatga aattggattt gcagtatatt gataattggt cgctgtggct
     5461 ggatgccaag atcttgctta agactattcc agcagtgacc aaagggaccg gagcaaaatg
     5521 aacggatcgc gtttttttaa aagggcctgg gatagaaaga aagggagctg gtttataaag
     5581 tttctccttt tcgtttcgta tttctttgct ctttccttga gtctggttat ctcttatctt
     5641 tgtgttagga agtatgactg gatcaaagaa ccctccttgg tgatatcctg ggtagtagtg
     5701 gtatccattt tcttgatagt ttttcttctg caagggtttt accagcagag aatggcggcc
     5761 agtaaaaaaa gggaggccct actgataatc aagggggtca tggtaggctc aatacttttg
     5821 gctattttag tctttgtttc taatggaaag ttggttttca atcaacgtga ggggattttg
     5881 tttttcttcc tgtccagctt ggtcttgctt gctctcttca ggtcaatact cttctttcac
     5941 ctgagaaggc acctgctgaa aaaggggatc ggcgtgacta atgtgctgat tgtgggagca
     6001 aatccggagg caaaagaatt ttcaaagagt ttagagctaa tcgagccatt caaatacagg
     6061 atagctggtt tagttgaaaa ggaagagaaa ataaggacgg acagctataa tggtttcaag
     6121 atcttaggta acttgaagga tatttgcaag ttaatagacc agtacaagat aagtgaaata
     6181 tacataacca gtgaatcgct tttacccata gaagttttga tggtagttga agagtgcagg
     6241 aacttgattg ggaggatata tattgccgca gagatgttcg aagtcctaag cgagaggata
     6301 aatctgggca agttaaaagg ggttcaactc ttgaagataa gataaacgag gcagtcccca
     6361 aaacatcaaa tcagtctaat aaaaactccc ttgtttccga tataaagaaa agaggtttga
     6421 aatccaaatt caagctattg gagggataaa atgaagttac agagaagatg cccactaatc
     6481 atatttatgg taagtttgac actattcctt ttcaacttgg cttggccgta tgatactcct
     6541 ggaacaagtg tcagagccac aggcatgggt ggtagtttca cagctctagc taatgaccct
     6601 agcgcggtct tttataatcc agccggtctg gttcaactga aaggggtgca gttctacctg
     6661 atgtatgaca gaaagacggt gtatggtgtg aatagcgcag aatcccctta tctgggagca
     6721 ggagcgataa gtttcccctt gtcccgggat atagttattg ccctggctgg atatcaggat
     6781 ggctcctcgg cagatccaac cggaatagta accaagaaca tcgggctctt gagcttttcc
     6841 agctgggtta cagaagagct ttcattcggg ctcaatctta aagggttata caattccaac
     6901 tacggaaaga aaaaggggtt cgatttcgat ttgggccttc tttatcggcc ccatccttgg
     6961 cttggcattg ggattatggg ggagaatctt gccaggaccg atatggtccc ggatatttcc
     7021 tccggtgttg taagcggtta tcagactcgc caagttaagt tgggattggc ttatcactat
     7081 gaaggtaaga attatgagac gtgcttagct ttagataaca ccatcaaaga tgcaaatcaa
     7141 ccaatacaga agagttacaa cttaaccagt atagggatgg aacaatggat attgacggac
     7201 aataagttaa acttagcgtt aagaggcggt tacagttttg gaaaagatta tgaattgaat
     7261 tatagtcaac ctgctttcgg aggttcaatc agatacaaag cacaagatta cactattcaa
     7321 ctggattata gctggtccaa gtatccttat gaatcggaag aggatttggc tggtgaccac
     7381 agggttggct tggtcattag tttagagaaa ggcgtcaaaa ggggtgagaa attgcttctc
     7441 aaagaagaga tgaaagaaga gaaaaaagaa gaagcgatag tagagaagaa ggaggcacct
     7501 atcactctgc ccagcgcgcc gattgaagag aaaaaagaaa gctatcctgc taagccagag
     7561 ccggaattct tacctgaaga aaaggatagc actaagggct ctcccgagat tctaacatac
     7621 agtctcaatt cagaaataga gttcctctcc gtgggcaaga acagatcggt aatgctcctg
     7681 ctcaaacccg aaatcagcca ggaaatagaa agatggaagc tttatatctg gagtgcctta
     7741 cccaccggtt ggagcaaaga ggaagttgaa aaatccctgg tgaagacttt tgagggatgg
     7801 ggcaatcctt cctttggagt aatgtgggat tgcagataca atgatgtcaa agtaaaaagt
     7861 ggaacttact attacgcctt agcgttgaag gacaatcaag gcgcgaaatg gtatagccag
     7921 ataaaatctt tcaagctgga ttagttaatg caaacgcatt aatttacgtt aaatgaagaa
     7981 gcgcacggca gagcggtgcg gtatcggaat tcctcctcgc cattcatcct cgccgcagag
     8041 cggcgaggtg ttctggcgaa ggagaataaa tctcccctcg ccctcgcctc gattcggccc
     8101 gcctcggcga gccatcggcg agacgggcga agcgggcctc ttttccaaag aggggaaaaa
     8161 agaggtctcc cccttcgaaa aagggggatt tgatggttcg atatg
//
LOCUS       DASWLN010000079         4301 bp    DNA     linear   ENV 20-FEB-2024
DEFINITION  MAG TPA_asm: Candidatus Zixiibacteriota bacterium isolate
            SMAG_U2949 k141_458660, whole genome shotgun sequence.
ACCESSION   DASWLN010000079 DASWLN010000000
VERSION     DASWLN010000079.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37960594
            Sequence Read Archive: SRR10376091
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Candidatus Zixiibacteriota bacterium (soil metagenome)
  ORGANISM  Candidatus Zixiibacteriota bacterium
            Bacteria.
REFERENCE   1  (bases 1 to 4301)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 4301)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (26-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/12/2024 08:28:26
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 1,440
            CDSs (total)                      :: 1,416
            Genes (coding)                    :: 1,412
            CDSs (with protein)               :: 1,412
            Genes (RNA)                       :: 24
            rRNAs                             :: 1 (16S)
            partial rRNAs                     :: 1 (16S)
            tRNAs                             :: 21
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 4
            CDSs (without protein)            :: 4
            Pseudo Genes (ambiguous residues) :: 0 of 4
            Pseudo Genes (frameshifted)       :: 0 of 4
            Pseudo Genes (incomplete)         :: 3 of 4
            Pseudo Genes (internal stop)      :: 1 of 4
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..4301
                     /organism="Candidatus Zixiibacteriota bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_458660"
                     /isolate="SMAG_U2949"
                     /isolation_source="soil"
                     /db_xref="taxon:2053527"
                     /environmental_sample
                     /country="USA: Oregon"
                     /lat_lon="42.99 N 123.63 W"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            <1..710
                     /locus_tag="VGB16_02650"
     CDS             <1..710
                     /locus_tag="VGB16_02650"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=3
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEX9916613.1"
                     /translation="TGNLVGYSDCKTSRIETTTDSTPPNQECIEYQYDGDSLLLLKHI
                     NTGFNCCPDTIIAGINIKDSLITIQESQSLENGGCKCLCLFDLNFEIRNLEPGEYTIK
                     VISPYIHPDEEQLEFSTTLSSSPSSGRYCVERNHYPWGNTEENPDWINKLIATFASEP
                     VGNPPQSIWQYEYKGQRVYYIPPQCCDQFSTLYNAAGSVICAPDGGYTGEGDGRCTDF
                     FKERKNEKLIWQDSRTN"
     gene            811..1866
                     /locus_tag="VGB16_02655"
     CDS             811..1866
                     /locus_tag="VGB16_02655"
                     /inference="COORDINATES: protein motif:HMM:NF027787.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF5050 domain-containing protein"
                     /protein_id="HEX9916614.1"
                     /translation="MKGLRIFFLFGVIILLGILLGCEETIVSHKPQDQRIVFYSNRTD
                     STEVFVMEQDGSNQERVTPSGLNIFPVGRAPLWSPDKEEIAFACLLNLPMPAIMLINP
                     DGTGLDTLVLHPGGNVLLGDWSPNENQIVYSAIPEFWMPPDPIEIFIINSDGTGNLKL
                     DDGYQPRFCGNNKVVYTDYYGGGNIFIINTDGTGKKQLTDVGVGGGAYHMPVGSPDGR
                     KIAFERSYSHLPSNPALGIMNSDSSGETLLVSGLAEGIAEIEFSPNGQRILFLTDNGD
                     NSEICVINIDGTGLDSLTGGIACGDGGASWSPDGEWIAFTSNRDGNKNIYRVSTDGRK
                     ELVQLTDNPADDFNPDW"
     gene            2076..3002
                     /locus_tag="VGB16_02660"
     CDS             2076..3002
                     /locus_tag="VGB16_02660"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_012843818.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003824 - catalytic activity [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="NAD-dependent epimerase/dehydratase family
                     protein"
                     /protein_id="HEX9916615.1"
                     /translation="MARIVVTGGAGFIGSHVADEFIRQKHRVVIIDNLSTGFEKNINP
                     RAKFYKVDINNSVIDKIFKREKPDILCHHAAQIDVRKSVENPISDAQGNILGSLNLFE
                     SCVRHKVKKVIFASTGGAIYGEQAYFPADESHPAKPLSPYGIAKLSIESYLYFYAEVY
                     GLNYICLRYANVYGPRQNSWGEAGVVAIFARKMLNDDKAFINGDGKQTRDFVYVNDVV
                     KANLLALDYPKNNTFNIGTGIETDINTIFRLIKKETGNNQKELHLPAKKGEQKRSVLD
                     FSRARKDLKWRPKMDLAEGIKKTVEYFKDERC"
     gene            2989..3768
                     /locus_tag="VGB16_02665"
     CDS             2989..3768
                     /locus_tag="VGB16_02665"
                     /inference="COORDINATES: protein motif:HMM:NF024118.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="MBL fold metallo-hydrolase"
                     /protein_id="HEX9916616.1"
                     /translation="MSDAKYTILGSSSGKASPNRFCTSILLETLETNILLDVGEGSTF
                     SLLRQGVNINSIKHVFISHMHSDHISGFPLLVQTMYLEGRKDDLDVYLPSEAVAGMNR
                     YMMMLYLFDEKIGFKINYHSIGFQYLYQDSNIKVKAYPNRHLLGYEKVIQDLFLPLKM
                     ESFCFGIESYGKRTIFSGDIGSLDDLNIIIQDVDLLICESMHIELFKLFGFLKEKQPK
                     KVILIHIPVDLEPKTRMILQEAKDNGMENIEIAYDGLVVEL"
     gene            3887..>4301
                     /locus_tag="VGB16_02670"
     CDS             3887..>4301
                     /locus_tag="VGB16_02670"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_012191394.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016491 - oxidoreductase activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="aldehyde dehydrogenase family protein"
                     /protein_id="HEX9916617.1"
                     /translation="MPDRVFKNYINGEWVDSSSGRTFENRNPADTREILGLFQKSNEK
                     DVQMAIGAAKEAYKKWRLFPAPRRAEILFKLVEILIKKKEEFARQMTQEMGKILKETR
                     GDVQEAIDMTYYMAGEGRRQFGQTTPSELPNKFMMS"
BASE COUNT         1323 a          794 c         1014 g         1170 t
ORIGIN      
        1 caactgggaa tctggttggt tacagtgatt gcaagacatc ccgaatagaa actactacag
       61 atagcacacc gccaaatcag gagtgcatag aatatcagta tgacggggac agccttcttc
      121 tgcttaaaca cataaatact gggttcaatt gttgtccgga tacaatcata gctggtatca
      181 atattaagga cagtcttatt acaatacaag aaagccaatc tcttgaaaac ggcggatgca
      241 agtgtctttg cctttttgac ttaaacttcg aaatcagaaa tttagagccc ggggagtaca
      301 cgataaaagt aatctcgcca tatatacatc cagatgaaga acagttggag ttcagtacca
      361 ccctttcttc ttcaccttct tcgggcagat attgtgtaga aagaaatcat tatccatggg
      421 gtaatactga ggagaatcca gattggatca ataaactgat cgcaacattc gcaagtgaac
      481 cagttgggaa tccaccacaa tcaatatggc aatacgaata taagggtcaa agggtgtatt
      541 atattccccc tcaatgttgc gaccagttca gcacacttta caatgcagct ggtagcgtga
      601 tatgtgctcc ggatggcgga tacactgggg agggtgacgg acggtgcact gattttttca
      661 aagagcgaaa gaatgaaaaa ctcatttggc aagactcacg aacaaattga gaatagtggg
      721 tagggaacgg ttgcaccgtt cccgaaaaga taaaagacag tagaagtaat ggcgcagcca
      781 ttacttaccc gattaaaaca ggagggtaaa atgaaaggtt taagaatctt ttttcttttc
      841 ggagtgatta ttcttttggg gattcttttg gggtgtgagg agactatagt tagccataaa
      901 ccccaagacc aaaggatagt tttctattct aaccgaactg acagcacaga ggttttcgtg
      961 atggagcaag atgggtcgaa tcaagagcga gtaacccctt caggtctaaa tatttttcct
     1021 gtaggtcgcg cccctctgtg gtcccccgac aaagaagaaa tcgcttttgc ttgcctcctt
     1081 aatctgccta tgccagctat tatgttaatt aatccagatg gaactggact tgacacttta
     1141 gtgcttcatc ccggtggaaa tgtattatta ggagactggt ctccgaatga aaatcagata
     1201 gtatattctg ctataccaga attttggatg cctcccgacc ctattgagat ttttataata
     1261 aattcggatg gaactggtaa tcttaaatta gatgatggtt atcaacccag attttgtgga
     1321 aataataaag tagtttatac agattattat ggaggaggca acatcttcat aatcaacact
     1381 gatggaactg gaaagaaaca gttgactgat gtgggtgtag gcggaggagc ctatcatatg
     1441 ccagttggtt caccagatgg acgaaaaatc gcttttgaga ggagttacag ccatcttcct
     1501 tccaatcccg ctttaggtat aatgaattca gatagctcag gggaaacact ccttgtgtcc
     1561 ggattggctg aaggtatagc agagatagag ttttcaccta atggtcaaag gatcttgttt
     1621 ttgaccgata atggagacaa tagtgaaatt tgtgtgataa atatcgatgg cactggttta
     1681 gattcactta ctggcggaat tgcctgtggg gatggcgggg caagctggtc accagatgga
     1741 gaatggattg cttttacctc gaatagagat ggcaataaga acatatacag ggtaagtact
     1801 gacggtcgaa aagagttagt ccaactaaca gataacccag cagatgattt taatccggat
     1861 tggtgatctg atataacact ataagggtcg gatttatccg gcccgtggcg ggatttatcc
     1921 gacccgaaaa cagcgggttc gatgaatcga acccctacat taagtctttt aaccccaatc
     1981 tttcaaaaag attggggttt ttgttgactt tgttttttaa atagttataa tattgttcta
     2041 aatttcactt gctctattgt tggaggagga aggccttggc tcgtatcgtt gtcaccggtg
     2101 gtgcggggtt tattggctcg catgtggcgg atgagtttat ccgacagaaa catagggttg
     2161 tcatcattga taatctcagc accggttttg agaagaacat aaatccccga gccaaattct
     2221 acaaagtgga tatcaacaat tcagtcattg ataaaatatt caaaagagaa aaaccggata
     2281 ttctttgcca tcacgcagcc cagatagatg ttcgaaagtc agttgagaat ccgatctcag
     2341 atgcacaagg gaatattctg ggaagcttga atctttttga atcgtgcgtg agacataaag
     2401 taaaaaaagt cattttcgcc tcaactggtg gggcaatcta tggagagcag gcatattttc
     2461 cagcagacga gagccatcct gctaagccgc tctctcccta cggcatagcg aaattatcca
     2521 ttgagagtta tctgtatttt tatgctgaag tatacgggtt aaattacata tgtctacgtt
     2581 atgccaatgt ttatggtcca aggcaaaact cttggggaga agcaggggtg gtggcgatat
     2641 ttgccagaaa gatgcttaat gatgataaag cttttatcaa tggagacggg aaacagacaa
     2701 gggattttgt ctacgtaaat gatgtagtca aagcaaacct tctagcttta gactatccga
     2761 agaacaatac cttcaatatt ggcaccggaa tagagaccga tatcaatact atttttagat
     2821 tgataaagaa agagaccgga aacaatcaga aggaactcca tcttcctgcc aaaaaaggag
     2881 agcagaagcg tagcgtttta gatttctcca gggccaggaa ggaccttaaa tggagaccca
     2941 aaatggactt agccgaaggt attaagaaaa cagtggagta ttttaaagat gagcgatgct
     3001 aaatatacga tcttagggtc atcttctggc aaggcttcac ccaatcggtt ttgcacctcg
     3061 atattgctcg aaacactcga gactaatata cttcttgatg taggagaagg aagcaccttc
     3121 tctcttttgc gtcagggagt gaatatcaac agcatcaagc atgtcttcat atcgcatatg
     3181 cattctgacc acatcagcgg ttttccgctc ttggttcaga ctatgtattt ggaaggaaga
     3241 aaggacgatt tagatgttta tcttccgagt gaggctgtgg cagggatgaa tcggtatatg
     3301 atgatgcttt atctttttga cgagaaaatc ggtttcaaga taaattatca ttcaatcggg
     3361 ttccaatatc tttatcagga ttcaaatata aaagtaaaag cctatcccaa tcgacatctt
     3421 ttaggatacg aaaaggtaat acaagacctt ttcttgccct tgaagatgga gagcttctgc
     3481 tttgggatag agagttatgg caagaggacc atcttttcgg gagacatcgg ctctttggat
     3541 gacctcaata tcattattca agatgtggat ctgctcatct gtgaatctat gcacatagag
     3601 ttgttcaagc tgtttgggtt cttaaaggag aaacagccca agaaggttat cttgattcat
     3661 atcccggttg acctggagcc caagaccaga atgatacttc aggaagccaa ggataatggt
     3721 atggagaaca tagaaatcgc ctatgatgga ttagtggttg aattgtaaat tagcttgcta
     3781 tgaaaaagtg ggtagggaac ggttgcaccg ttcccgaaaa gataaaagac agtagaagta
     3841 atggcgcagc cattacttac ccgattaaaa tccttgagga ggttttatgc ccgacagggt
     3901 tttcaaaaac tacataaatg gcgagtgggt tgactcatcc tctggcagaa ctttcgaaaa
     3961 cagaaacccg gctgatacta gagagatatt gggcttattt cagaaatcaa atgaaaaaga
     4021 tgtgcagatg gctattggtg ccgccaagga agcttacaag aaatggcgtc tgttcccagc
     4081 cccgcgcagg gctgaaatcc ttttcaagtt agtggagatt ctgattaaaa aaaaggagga
     4141 gttcgcccgc cagatgaccc aggagatggg gaagatatta aaagaaactc gcggggacgt
     4201 tcaagaagcg attgatatga cctattatat ggcgggagag gggaggaggc agtttggaca
     4261 gactacgcca tctgaactgc ccaacaagtt tatgatgtca a
//
LOCUS       DASWLN010000080         4899 bp    DNA     linear   ENV 20-FEB-2024
DEFINITION  MAG TPA_asm: Candidatus Zixiibacteriota bacterium isolate
            SMAG_U2949 k141_1177865, whole genome shotgun sequence.
ACCESSION   DASWLN010000080 DASWLN010000000
VERSION     DASWLN010000080.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37960594
            Sequence Read Archive: SRR10376091
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Candidatus Zixiibacteriota bacterium (soil metagenome)
  ORGANISM  Candidatus Zixiibacteriota bacterium
            Bacteria.
REFERENCE   1  (bases 1 to 4899)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 4899)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (26-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/12/2024 08:28:26
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 1,440
            CDSs (total)                      :: 1,416
            Genes (coding)                    :: 1,412
            CDSs (with protein)               :: 1,412
            Genes (RNA)                       :: 24
            rRNAs                             :: 1 (16S)
            partial rRNAs                     :: 1 (16S)
            tRNAs                             :: 21
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 4
            CDSs (without protein)            :: 4
            Pseudo Genes (ambiguous residues) :: 0 of 4
            Pseudo Genes (frameshifted)       :: 0 of 4
            Pseudo Genes (incomplete)         :: 3 of 4
            Pseudo Genes (internal stop)      :: 1 of 4
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..4899
                     /organism="Candidatus Zixiibacteriota bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_1177865"
                     /isolate="SMAG_U2949"
                     /isolation_source="soil"
                     /db_xref="taxon:2053527"
                     /environmental_sample
                     /country="USA: Oregon"
                     /lat_lon="42.99 N 123.63 W"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            169..789
                     /locus_tag="VGB16_02675"
     CDS             169..789
                     /locus_tag="VGB16_02675"
                     /inference="COORDINATES: protein motif:HMM:NF020893.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_process: GO:0055085 - transmembrane transport [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="DedA family protein"
                     /protein_id="HEX9916618.1"
                     /translation="MSEFFSASYEDLLFLLTRYGAVWFYVFLFLSSVTESLFPPYPGD
                     TVAFAGGLLAISGLLNPIFTFFSVCFGGLIGCLILYSLGQTGGRKVFMHNRGWFLNRQ
                     QLEKIENWFGRYGNWVIVASRFLPGVRSGVALAAGVGNVRLPFMAAYSLISIMLWNLL
                     IMLSATLIHNNWRKLYDLTRIYNGVVLAILVVVIIIGIIILKRKNR"
     gene            786..1829
                     /locus_tag="VGB16_02680"
     CDS             786..1829
                     /locus_tag="VGB16_02680"
                     /EC_number="6.3.2.4"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003114722.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_function: GO:0008716 - D-alanine-D-alanine ligase
                     activity [Evidence IEA];
                     GO_function: GO:0046872 - metal ion binding [Evidence
                     IEA];
                     GO_process: GO:0009252 - peptidoglycan biosynthetic
                     process [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="D-alanine--D-alanine ligase"
                     /protein_id="HEX9916619.1"
                     /translation="MKKGNFKVLVLMGGSSAERDVSLASGEAVVGALKEAGYKVMALD
                     TAQGNKLLADNGKYLNGGVKTKPPDTKDLQKSESKLTLKAIESFDFSDVDVIFLALHG
                     GSGENGTIQALLDMTGKPYTGSGMLASALCMDKAMSKKIFERDGIPTPRWFLLKEEKL
                     TDITTIETLIESNLGFPVVVKPNDQGSTIGFSIVSSPSKLKEAIQRAEHYSNEVLFEE
                     YIPGRELTVGIVEDQALPVIEIVPEHGVYDYECKYTKGKSKYVCPAELPKHKAEEIQA
                     YGLKAFKSLKCEGYARVDFRYSENERFYCLEVNSLPGMTATSLVPKAAKAMGIEFPEL
                     IDRIVQSALNKRR"
     gene            1836..2570
                     /locus_tag="VGB16_02685"
     CDS             1836..2570
                     /locus_tag="VGB16_02685"
                     /EC_number="3.1.3.-"
                     /inference="COORDINATES: protein motif:HMM:NF012905.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016787 - hydrolase activity [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="HAD family hydrolase"
                     /protein_id="HEX9916620.1"
                     /translation="MLKAVIFDMGSTLIEFENHSWDVLGKMGTASGYEFLKRQNISLP
                     EYNVFEAMLVRKLEQRLSSIKENLKEIKFEEVVFPFFEELKISTEDGKSSNFLYEYYK
                     PITQQATLIEDAVEILSSLKEEGLKVGLISNTIFPGDYHRDELKRFGLYPFLEISLFS
                     CEFGYRKPHPEIYLECLKKLKIEAEEAVFVGDRLIEDVAGPKSLGMKAILKYKEGRDY
                     SLPIQPDAKVHSLTQLPQAIRNLKWD"
     gene            2561..3955
                     /gene="cysS"
                     /locus_tag="VGB16_02690"
     CDS             2561..3955
                     /gene="cysS"
                     /locus_tag="VGB16_02690"
                     /EC_number="6.1.1.16"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_013944043.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0005737 - cytoplasm [Evidence IEA];
                     GO_function: GO:0004817 - cysteine-tRNA ligase activity
                     [Evidence IEA];
                     GO_process: GO:0006423 - cysteinyl-tRNA aminoacylation
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="cysteine--tRNA ligase"
                     /protein_id="HEX9916621.1"
                     /translation="MGLKVYNTLTRKREAFKPIVSGKVGLYTCGPTVYDFAHIGNFRA
                     YVFGDLLRRYLEYKGYEVKQVMNITDIDDKTIKGSQEKGIPLTQYTMKFKKAFFEDME
                     ALNIKKAHVYPEATSHLKEMIEFVKKLLDKGNAYRMDNSVYFKISSFPEYGKLSKMKL
                     DELKTTERVLADEYEKEQIADFALWKGWEEIDGDVFWITDFGKGRPGWHLECSVMSMK
                     YLGNTVDIHTGGVDLIFPHHENEIAQSESVTGKKFVNYWLHNEFLMVEGKRMAKSLGN
                     YYTLRDLLAKGYNPKAIRYLLLSTHYRQQLNFTFEGLKSAEGALNRLFDFIHNLKNIK
                     DGKNNPKIKKLIRETKKEFEEALDDDLNMSQALSKIFDLVKEVNLLIERNHISESDAK
                     ALISLLVEFDNVLGVLERVESIDEEMQGLIYQREEARRDENWELADKIRKQLEDKGII
                     LEDTSQGTKWKKKL"
     gene            4006..4077
                     /locus_tag="VGB16_02695"
     tRNA            4006..4077
                     /locus_tag="VGB16_02695"
                     /product="tRNA-Thr"
                     /inference="COORDINATES: profile:tRNAscan-SE:2.0.12"
                     /note="Derived by automated computational analysis using
                     gene prediction method: tRNAscan-SE."
                     /anticodon=(pos:4038..4040,aa:Thr,seq:tgt)
     gene            4209..4580
                     /locus_tag="VGB16_02700"
     CDS             4209..4580
                     /locus_tag="VGB16_02700"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_018060546.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="four helix bundle protein"
                     /protein_id="HEX9916622.1"
                     /translation="MGKEQKDFNFRNLIVWQKAVDLVDRIYSLTGSFPQSENKILIPQ
                     LLRAVNSIPSNIAEGNGRKSRKEFVQYLYFAKGSLAETLTFLEIAKRRNYLDMSDYIE
                     INEACVEIQKLIVALINKYSG"
     gene            4686..4767
                     /locus_tag="VGB16_02705"
     tRNA            4686..4767
                     /locus_tag="VGB16_02705"
                     /product="tRNA-Tyr"
                     /inference="COORDINATES: profile:tRNAscan-SE:2.0.12"
                     /note="Derived by automated computational analysis using
                     gene prediction method: tRNAscan-SE."
                     /anticodon=(pos:4720..4722,aa:Tyr,seq:gta)
BASE COUNT         1546 a          848 c         1181 g         1324 t
ORIGIN      
        1 accatctctt cgagcctgag ccttccttcg acactgagcc tcaggacgaa gtgcagagcc
       61 gaaggcctga gcaaagtcga agggaggggg attttaaaat agattgcata ttgctctaat
      121 atgtaacgac atttaatgct ataattaacc aaggaaaaag ataataggat gtcagagttt
      181 ttctcagcca gttacgagga tttgcttttc ttattgacca gatatggcgc agtctggttt
      241 tatgtttttt tgtttctcag ctcagtcaca gaaagtcttt ttccgcccta ccctggagat
      301 accgtagctt ttgctggtgg tcttctggct atctctggct tgctcaaccc tattttcact
      361 ttcttttcag tctgttttgg cggattaatt ggctgcctga tattatattc ccttggtcag
      421 accgggggaa gaaaggtttt tatgcataat agaggttggt ttctcaacag gcagcagttg
      481 gagaaaatag agaattggtt tggaagatat ggaaattggg tcatagtagc cagcaggttt
      541 ctaccgggtg tgcgctctgg agtggcgttg gcggctggag tgggaaatgt aagattaccc
      601 tttatggcag cttacagctt gattagcata atgctctgga atctgcttat aatgctctct
      661 gcaaccttga tacataataa ttggagaaaa ctatatgact tgacgagaat ttataatggg
      721 gtagtgttgg caattctcgt ggtcgtcata atcatcggga taattatatt gaaaaggaag
      781 aacagatgaa aaaagggaac tttaaggttt tggtcttgat gggcggcagt tctgctgaaa
      841 gggatgtctc cttggcaagc ggagaagccg ttgtaggagc tcttaaggaa gccggataca
      901 aagttatggc tttagatact gctcaaggaa ataaactgtt agcggataat ggaaagtatt
      961 tgaatggtgg ggtgaaaacc aagccccccg atactaaaga cctacaaaaa tcagaaagca
     1021 aattgacttt gaaagcaatt gaaagtttcg atttctcgga tgtggatgtg atatttttgg
     1081 ctttgcatgg cggttcaggc gaaaacggaa caattcaagc tttattggat atgaccggaa
     1141 aaccctatac tggttcaggc atgcttgcca gtgctttgtg tatggataag gcgatgtcca
     1201 aaaagatatt tgaaagggat ggaatcccta ccccgcgctg gtttctgttg aaagaggaaa
     1261 agctgactga tattactact atcgaaactt taattgaaag caacctcggc ttcccagtgg
     1321 tggtgaagcc caatgaccag ggctcaacca taggtttttc aatcgttagc tctccttcca
     1381 aattgaaaga ggcaatccaa agagccgagc attactctaa cgaagttcta tttgaagagt
     1441 acattccagg aagagaattg accgttggga tagtggagga ccaagccctg ccagtaatag
     1501 agatagtgcc ggaacacgga gtgtatgatt acgaatgcaa atatacaaag ggaaaaagta
     1561 aatatgtctg tccggcagaa ctgcccaagc acaaagccga ggagattcag gcgtacggct
     1621 taaaagcttt caagagtctg aaatgtgaag gatatgcccg ggttgatttc agatatagcg
     1681 aaaatgaaag attctattgt ttggaagtga actcgcttcc cgggatgact gccaccagct
     1741 tggttcccaa ggcagccaag gctatgggca tagagtttcc cgaattgatt gatagaatcg
     1801 tgcaatcagc attaaacaag agaaggtgat aaaacttgct aaaggcagtc atatttgata
     1861 tgggtagcac cctgattgag tttgaaaatc acagctggga tgttctggga aaaatgggaa
     1921 cagccagtgg atacgagttt ttaaagagac aaaatataag cttgcctgag tacaacgtat
     1981 tcgaggcgat gttggtcaga aagctcgagc agagattatc cagcataaag gaaaacttaa
     2041 aggaaataaa gtttgaggag gttgtttttc catttttcga agagcttaaa atcagcacag
     2101 aagacgggaa atccagtaat ttcttgtatg aatattacaa gcctataacc cagcaagcta
     2161 ctttaataga agatgcagtc gaaattttga gttctctcaa agaagaaggg cttaaggttg
     2221 gcttaatctc gaacactatc tttcccgggg attaccacag agatgagctt aaaaggttcg
     2281 ggctttatcc ttttttggaa atatccttgt tctcctgtga attcggttat cgcaagcccc
     2341 accctgagat ttacctggag tgcttgaaaa aacttaaaat agaagcagaa gaagccgttt
     2401 tcgttggcga tagacttatc gaagacgtgg caggaccaaa aagtttaggt atgaaggcga
     2461 tcttgaaata taaagagggc agggattatt ctcttcccat tcagccggat gcaaaggtac
     2521 atagtttaac tcaattaccc caagccataa ggaacttaaa atgggactaa aggtatataa
     2581 tacccttacc agaaaaagag aagctttcaa accaattgtg tcgggaaagg tcgggcttta
     2641 cacttgtgga cccacagttt atgatttcgc acacattggt aacttcagag catatgtttt
     2701 tggcgactta ctgagaagat acctcgaata taaaggttat gaagtcaagc aggtgatgaa
     2761 cataacagat atcgatgaca agaccataaa aggctcacag gaaaaaggga tacccctaac
     2821 tcagtacacg atgaaattca agaaggcatt ttttgaagat atggaagcgc ttaacataaa
     2881 aaaagcccac gtttatcctg aagccaccag ccacctaaag gaaatgatag agtttgtgaa
     2941 aaaactttta gataagggaa atgcctaccg gatggacaac tcagtctact ttaaaatctc
     3001 ctctttccct gaatacggaa aactgtccaa gatgaaattg gatgaattga aaacaaccga
     3061 gagggttttg gcggatgagt acgaaaaaga acagattgcc gatttcgcct tatggaaggg
     3121 atgggaggaa atagatgggg acgttttctg gataactgat tttggaaaag gaaggccggg
     3181 ctggcatttg gagtgctcgg tgatgagcat gaaatatttg ggaaataccg tagatatcca
     3241 tactggtgga gtggatttga tatttcctca ccacgaaaac gaaatagccc agagtgaatc
     3301 agttactggc aagaagttcg tgaactattg gctgcataac gaattcctta tggtggaagg
     3361 gaagaggatg gcgaaatctt tggggaatta ttacacctta agagacctat tagccaaggg
     3421 atataacccc aaagccattc gttatctctt actatccact cattaccgcc aacaattgaa
     3481 tttcactttt gaggggctga aatcagctga aggggcgctc aatcggcttt tcgacttcat
     3541 acataatctc aaaaacataa aggatggcaa aaataatcct aaaatcaaga aactgatacg
     3601 cgagaccaaa aaggagtttg aagaggcttt ggatgatgat ttgaatatgt ctcaagcgct
     3661 aagcaagatt tttgatttag tcaaagaagt caacctactt atagaaagga atcacatttc
     3721 cgaatcggat gcaaaagcct taatctccct tttagtggaa ttcgataatg tgttaggagt
     3781 tctggagagg gtggagtcaa ttgacgagga aatgcaaggc ttgatttacc aaagggaaga
     3841 agccaggaga gatgagaatt gggaattagc cgataaaata agaaagcagt tggaagataa
     3901 gggaataatt ttggaagata cttcccaagg caccaagtgg aagaagaagc tatagcgatt
     3961 gaaactgttg acaagagaat acgtggctgt tatattttgt ccactgccgg cgtaactcag
     4021 tggtagagta gctgatttgt aatcagccag tcaggggttc aagtcccttc gccggctcat
     4081 taaggcagtt tcgagaaacg agttttgagt ttcgagacca cgcaacccac cctttcttga
     4141 gggaagggtt ggagggtctc aaaacctgag actcaaaact gaattaaaag agctttgaga
     4201 ggatagtttt gggaaaagaa caaaaggact ttaattttcg taatttaata gtctggcaga
     4261 aagcagtcga tttagttgac aggatttatt cattgacagg gtcttttcct cagagtgaga
     4321 ataaaattct aattcctcag ctgttgaggg cagttaattc aataccatct aacatagctg
     4381 aagggaatgg caggaagagc agaaaggaat ttgttcaata tctttatttt gcaaagggct
     4441 ctctggcaga gacattgact tttttggaaa tcgctaaaag aagaaactac ttggacatgt
     4501 cagattatat tgagataaat gaagcttgtg ttgagattca gaaattgatt gttgcattga
     4561 taaataaata ttcaggataa ggttttcgat agggttttct taaaactcga aacttaaaac
     4621 tcaaaactgt ttttggatgg gtcgggtctc aaaactgaga acttaaaact taaaactgtt
     4681 ttattggagg ggttcccgag cggccaaagg gaacagactg taaatctgtc ggcgacgcct
     4741 tcggaggttc gaatcctccc ccctccagaa aagcagttta aaggtttagt agtttaggag
     4801 tttagagaac tgatgacctt ttaaacttct aaacttgtaa acttataaac tgtatttact
     4861 gcgggagtaa ctcagttggc tagagtcatc catctggat
//
LOCUS       DASWLN010000081         4525 bp    DNA     linear   ENV 20-FEB-2024
DEFINITION  MAG TPA_asm: Candidatus Zixiibacteriota bacterium isolate
            SMAG_U2949 k141_1177903, whole genome shotgun sequence.
ACCESSION   DASWLN010000081 DASWLN010000000
VERSION     DASWLN010000081.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37960594
            Sequence Read Archive: SRR10376091
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Candidatus Zixiibacteriota bacterium (soil metagenome)
  ORGANISM  Candidatus Zixiibacteriota bacterium
            Bacteria.
REFERENCE   1  (bases 1 to 4525)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 4525)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (26-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/12/2024 08:28:26
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 1,440
            CDSs (total)                      :: 1,416
            Genes (coding)                    :: 1,412
            CDSs (with protein)               :: 1,412
            Genes (RNA)                       :: 24
            rRNAs                             :: 1 (16S)
            partial rRNAs                     :: 1 (16S)
            tRNAs                             :: 21
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 4
            CDSs (without protein)            :: 4
            Pseudo Genes (ambiguous residues) :: 0 of 4
            Pseudo Genes (frameshifted)       :: 0 of 4
            Pseudo Genes (incomplete)         :: 3 of 4
            Pseudo Genes (internal stop)      :: 1 of 4
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..4525
                     /organism="Candidatus Zixiibacteriota bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_1177903"
                     /isolate="SMAG_U2949"
                     /isolation_source="soil"
                     /db_xref="taxon:2053527"
                     /environmental_sample
                     /country="USA: Oregon"
                     /lat_lon="42.99 N 123.63 W"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            complement(<1..640)
                     /gene="purQ"
                     /locus_tag="VGB16_02710"
     CDS             complement(<1..640)
                     /gene="purQ"
                     /locus_tag="VGB16_02710"
                     /EC_number="6.3.5.3"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011343995.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004642 -
                     phosphoribosylformylglycinamidine synthase activity
                     [Evidence IEA];
                     GO_process: GO:0009152 - purine ribonucleotide
                     biosynthetic process [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="phosphoribosylformylglycinamidine synthase I"
                     /protein_id="HEX9916623.1"
                     /translation="MKLGIIVFPGSNCDYDAYWVGKEVLGQQTTFLWHQDKDLGNCDC
                     VILPGGFSYGDYLRTGAIAKFAPIMKKVIRFAEEGGLVIGICNGFQILLEAGLLPGAM
                     LRNKDLRFICRYLYLRVENDKTPFTTSCQKNQVLKIPIAHSDGNYYISDEGLEKLKRN
                     DQIVLRYCDPQGRITDKANPNGSKYNIAGVCNKQRNVFGMMPHPERCSERVLG"
     gene            complement(637..903)
                     /gene="purS"
                     /locus_tag="VGB16_02715"
     CDS             complement(637..903)
                     /gene="purS"
                     /locus_tag="VGB16_02715"
                     /EC_number="6.3.5.3"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_020399315.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="phosphoribosylformylglycinamidine synthase
                     subunit PurS"
                     /protein_id="HEX9916624.1"
                     /translation="MAKRSSKEDVATVHVRLKEGVLDPQGLTIKRALEDMGYEGIGEV
                     KSGKFFEIKVSSKNRKESEERIDEICRKLLTNPVIERYTFEFDK"
     gene            complement(903..1607)
                     /gene="pssA"
                     /locus_tag="VGB16_02720"
     CDS             complement(903..1607)
                     /gene="pssA"
                     /locus_tag="VGB16_02720"
                     /EC_number="2.7.8.8"
                     /inference="COORDINATES: protein motif:HMM:TIGR00473.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003882 - CDP-diacylglycerol-serine
                     O-phosphatidyltransferase activity [Evidence IEA];
                     GO_process: GO:0008654 - phospholipid biosynthetic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="CDP-diacylglycerol--serine
                     O-phosphatidyltransferase"
                     /protein_id="HEX9916625.1"
                     /translation="MKDIIPGAFTLGNMFCGFLSVLFSVEGNLVTAAWLVVLAGFLDG
                     LDGKLAQALGSTSQFGIELDSFADVISFGLAPGVLFYSSEHIILGKWVWVLGFIFIAA
                     GAFRLVRFNLRTEFETKDFFNGLPITAAAMTLASYFLFSHGVWGEMRYVGIVILILLF
                     FSALMVSNIKYDNLPNFSFDNQWNRIKLLYVFVGIVAILIKPKIAPFPLGMMYVLSGV
                     IRGINDLFSTNRIRTN"
     gene            complement(1613..2284)
                     /locus_tag="VGB16_02725"
     CDS             complement(1613..2284)
                     /locus_tag="VGB16_02725"
                     /EC_number="4.1.1.65"
                     /inference="COORDINATES: protein motif:HMM:NF003685.0"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004609 - phosphatidylserine decarboxylase
                     activity [Evidence IEA];
                     GO_process: GO:0008654 - phospholipid biosynthetic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="phosphatidylserine decarboxylase family protein"
                     /protein_id="HEX9916626.1"
                     /translation="MITFLSGCFVAKEGLGLVLPAIFLTLVLWFLFWLNNHTAILFLA
                     VIFSVSTVLLIFFFRDPKRIIPGGENLILSPADGRVDEIEKLSSASFLETAAIRVSIF
                     MSLFDVHINRLPISGEVKYIEHKSGKSLPAFKKESAKENEQTEIGIDNLSFRVILRQM
                     VGFTARRIICHLKVGDKVKIGERFGMIKFGSRAELLLPQNVEIEVNLGDRVKAGETII
                     GIFKR"
     gene            complement(2250..3545)
                     /gene="purB"
                     /locus_tag="VGB16_02730"
     CDS             complement(2250..3545)
                     /gene="purB"
                     /locus_tag="VGB16_02730"
                     /EC_number="4.3.2.2"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_012647966.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004018 - N6-(1,2-dicarboxyethyl)AMP
                     AMP-lyase (fumarate-forming) activity [Evidence IEA];
                     GO_process: GO:0009152 - purine ribonucleotide
                     biosynthetic process [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="adenylosuccinate lyase"
                     /protein_id="HEX9916627.1"
                     /translation="MIPRYSLPKMQNIWSEQNKFEKWLEIEILACEAQAKFGNIPRSA
                     LLQIKRKAKFNAQRIEKIEEKTKHDVVAFLTNLAENIGPESKYVHLGMTSSDILDTSL
                     SVLMREAGQLIKEDLRNLKTIVKKKALKYKYTPIIGRTHGVHAEPTTLGLKFALWYDE
                     IKRDIERLERAIETISFGKISGAVGNFANIDPKVEKYVCQELGLKPAPVSNQILQRDR
                     HAEYMTTLAIIAGSIEKFCTEIRNLGRTEILEMEEPFTKGQKGSSAMPHKRNPILCER
                     LCGMARLVKSHALASLDNMALWHERDISHSSVERIIIPDSTILINYMLNKFIYMIDNL
                     MIYPENMLKNINSTRGLVFSQRVLLRLIEKGLTREVAYQVVQKNAMQAWETGKSFEDL
                     ISKDKETRKCLSLKEINSCFDIGFYLEKVDYIFKRVFRG"
     gene            complement(3547..4194)
                     /gene="fsa"
                     /locus_tag="VGB16_02735"
     CDS             complement(3547..4194)
                     /gene="fsa"
                     /locus_tag="VGB16_02735"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_006929619.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016832 - aldehyde-lyase activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="fructose-6-phosphate aldolase"
                     /protein_id="HEX9916628.1"
                     /translation="MKFFLDTANIDEIKEVASWGILNGVTTNPTLLAKEKGSFDSIVE
                     QICEIVDGPVNLEAINSEADSIVKEAKELARIHKNVVVKIPITKEGLKSIKLLSSIGI
                     KTNTTLVFSPIQALMAAKAGTSYVSPFVGRLDDASHVGMDLVGQILTIFKNYDMKTEI
                     IVASIRNPLHVVEAAMMGADIATMPFKVMSQLMQHPLTDIGIKRFLEDWKKVKER"
BASE COUNT         1242 a         1079 c          826 g         1378 t
ORIGIN      
        1 agcctaacac cctctcagaa catctctccg gatggggcat cattccaaaa acgtttcttt
       61 gtttattgca taccccggcg atattgtatt tggagccgtt ggggtttgct ttgtcggtta
      121 ttctcccctg cgggtcgcaa taacgcaaaa ctatctggtc attacgtttc aacttttcga
      181 gcccctcatc cgaaatatag tagtttccat cactatgggc tattggtatt ttaagcacct
      241 gatttttctg gcaagaagtg gtgaatggag ttttgtcgtt ttccaccctc aaatagagat
      301 atctgcagat gaaacgcaaa tccttattgc gcaacatagc cccgggcaaa agacccgctt
      361 ctaagagaat ctggaaccca ttgcagattc caatgaccag acccccttcc tcggcgaatc
      421 ggattacctt cttcattata ggcgcaaact tggcaatcgc accagtgcgc agataatctc
      481 cgtatgagaa ccctccgggc aaaatcacgc agtcgcaatt acccaagtct ttgtcctgat
      541 gccataagaa agtggtttgc tgacctaaca cttccttgcc cacccagtag gcgtcgtaat
      601 cgcaatttga gccggggaaa actatgattc ctaacttcat ttgtcaaact cgaaggtgta
      661 tctctcaatt accggattgg ttaataattt cctgcaaatc tcgtctattc tttcttctga
      721 ttctttcctg tttttggaac tcacttttat ctcaaagaat ttgcctgatt tgacctctcc
      781 aatcccctca taccccatat cctccagagc cctttttatg gtcaagccct gagggtcaag
      841 caccccctct tttaatctga cgtgtactgt cgccacatct tctttggagc ttcttttagc
      901 cattagtttg tcctgattct gttggttgaa aataaatcat ttattccacg aatgacacct
      961 gaaagaacat acatcatgcc cagaggaaaa ggagctattt tcggctttat caagatcgcc
     1021 actattccca caaacacata cagtaattta attctattcc attggttgtc aaaagaaaaa
     1081 ttcggcaggt tatcatattt gatattgctc accattagag cggaaaagaa aagaagaatc
     1141 agaatcacaa ttccgacata cctcatttct ccccaaactc catgggagaa gagaaaatag
     1201 ctagccaagg tcatagctgc agcggttata ggtagaccat tgaagaaatc ctttgtttca
     1261 aattcagtcc gcaagttaaa gcgcactaac ctgaaggcgc cagctgctat gaagataaaa
     1321 cctaaaaccc acacccattt gcccaagatg atatgctccg aggaatagaa aagcactccc
     1381 ggagccaatc caaaagagat aacatctgca aaggaatcca gctctatgcc gaattgagaa
     1441 gttgaaccca atgcttgagc taattttcca tctaagccat ctaagaaacc tgctaagaca
     1501 actaaccagg cggcagtgac caaattcccc tcaacactga aaaggacgga cagaaaaccg
     1561 cagaacatat ttcccagagt gaaggcgccg gggattatat ccttcatata tttcatcttt
     1621 tgaaaatccc tattatcgtt tcgccggctt tgactctatc gcctaagttg acttcaatct
     1681 ccacattctg cggcaacaag agttcagctc ttgagccgaa ctttatcata ccaaacctct
     1741 caccgatttt gactttgtct ccaaccttta agtgacagat aatccgacgc gcggtaaagc
     1801 caaccatctg ccttaagata actctgaagc tgagattatc tattcctatc tcagtttgtt
     1861 cgttttcctt tgcggattcc ttcttgaaag ccggaaggga tttaccggac ttatgctcta
     1921 tatatttgac ttctccactt atgggcaggc ggttgatatg cacatcaaac aaagacataa
     1981 aaatgctgac tcttatggct gcggtttcaa gaaaacttgc tgaagataat ttctcgatct
     2041 catctacgcg accatctgcc ggagataata taagattctc tccacctgga ataatcctct
     2101 tggggtcacg aaaaaagaaa attaataaaa cggtggatac ggaaaaaatg actgccaaaa
     2161 acaaaatggc tgtatgattg tttagccaga aaagaaacca cagaacaaga gtcaaaaata
     2221 tagccggcag aactaatccc aaaccctcct tagccacgaa acacccgctt aaaaatgtaa
     2281 tcaactttct ccaaataaaa accgatatca aaacaactat ttatctcttt taaagaaagg
     2341 cattttcttg tttctttgtc tttggaaatc aaatcctcaa acgactttcc tgtttcccaa
     2401 gcctgcattg cattcttctg taccacttga taagccacct cgcgagtcag tcccttttca
     2461 atcaaacgca aaagaactct ctgggaaaac acaagccctc ttgtagaatt gatattcttg
     2521 agcatattct caggataaat cattaagtta tctatcatat aaataaattt attaagcatg
     2581 taattaatca aaatcgtgct atctggaatt attatcctct caactgaaga atgactaata
     2641 tccctttcgt gccacaaagc catattgtcc aatgaagcta aagcatggga tttgaccagg
     2701 cgagccatac cacacagtct ttcacaaaga atcgggtttc tcttgtgtgg catagcggaa
     2761 gaccctttct gtcctttggt aaatggctct tccatttcca gtatttccgt tctcccgagg
     2821 tttcttatct ccgtgcaaaa cttttcaatt gagccggcaa tgatagccag ggtggtcata
     2881 tattctgcgt gtctgtctct ctgcaggatt tgatttgaaa ccggtgccgg cttaagtcca
     2941 agctcctggc aaacatattt ttctactttt gggtcaatat tggcaaaatt tcccactgca
     3001 cctgaaatct taccgaagga tatggtctcg attgctcttt ctaaacgttc tatatctctt
     3061 tttatttcat cataccataa agcaaacttt aatcctaagg tggtcggctc agcgtgaact
     3121 ccgtgagtcc gaccgattat cggagtatat ttgtatttca aggctttctt ttttactatg
     3181 gtcttgagat tacgcaaatc ctcctttatg agttgccctg cctctctcat caagacagag
     3241 agggatgtgt ctagaatgtc tgaggaggtc atacctaaat ggacatattt tgattcagga
     3301 ccaatattct ctgctaaatt ggttaaaaag gcaaccacat catgtttcgt cttttcctct
     3361 atcttttcga tgcgctgggc attgaatttg gcttttcttt tgatctgaag taatgccgaa
     3421 cgtggaatgt tgccgaattt tgcttgtgcc tcgcaagcca gaatttctat ctctagccat
     3481 ttctcgaact tattttgttc tgaccagata ttttgcattt ttggcaaaga atatcttggt
     3541 atcattttat ctctccttta cttttttcca atcctctaag aaacgcttta ttccaatatc
     3601 ggtcaggggg tgctgcatca attgcgacat gaccttaaag ggcatagtgg cgatatccgc
     3661 gcccatcatt gccgcctcga ccacatgtaa gggatttctt atgcttgcca cgattatctc
     3721 tgttttcata tcatagtttt tgaagatggt gagaatttgt cccactaaat ccatccctac
     3781 gtgactggca tcatccaatc tgcccacaaa tggactaaca taggatgttc ctgctttggc
     3841 tgccatcagg gcctggatag gggagaaaac caaggtggta ttggttttga ttccgattga
     3901 gcttaataac ttaatactct ttaacccttc ttttgtaatc ggaatcttga ccactacatt
     3961 cttgtgtatc ctggctaact cttttgcttc tttaactata ctatccgcct ccgaattaat
     4021 ggcttcaaga ttaactggcc catccacaat ttcgcaaatc tgctctacga tggaatcgaa
     4081 gcttcctttt tccttcgcta aaagtgtagg gttggtggtt accccattta aaatccccca
     4141 gctggctacc tcttttatct catcgatatt ggctgtgtcc aaaaagaact tcatatatcc
     4201 tcctttcaat actttgtctt cactgaatat aacttttata tgggtagctt tagtggcttc
     4261 cgccactgag agccaaccgc agaagctgtt aacctaagtt tctgtcattg cgagcgtagc
     4321 gaagcaatct cgctttacat aaatcaatga gttagggatt gctttgtctc ccgcctgtgg
     4381 cgggatcctc gcaatgacgg caaaaaccac tttttcaaca aaccctaaag gtttgtctct
     4441 acgttttgtt attactgttg tgtcaggact tccattcgac tctgctcagg ccttcggctc
     4501 tgaggctcag gctcgaagag atggt
//
LOCUS       DASWLN010000082         2296 bp    DNA     linear   ENV 20-FEB-2024
DEFINITION  MAG TPA_asm: Candidatus Zixiibacteriota bacterium isolate
            SMAG_U2949 k141_459132, whole genome shotgun sequence.
ACCESSION   DASWLN010000082 DASWLN010000000
VERSION     DASWLN010000082.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37960594
            Sequence Read Archive: SRR10376091
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Candidatus Zixiibacteriota bacterium (soil metagenome)
  ORGANISM  Candidatus Zixiibacteriota bacterium
            Bacteria.
REFERENCE   1  (bases 1 to 2296)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 2296)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (26-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/12/2024 08:28:26
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 1,440
            CDSs (total)                      :: 1,416
            Genes (coding)                    :: 1,412
            CDSs (with protein)               :: 1,412
            Genes (RNA)                       :: 24
            rRNAs                             :: 1 (16S)
            partial rRNAs                     :: 1 (16S)
            tRNAs                             :: 21
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 4
            CDSs (without protein)            :: 4
            Pseudo Genes (ambiguous residues) :: 0 of 4
            Pseudo Genes (frameshifted)       :: 0 of 4
            Pseudo Genes (incomplete)         :: 3 of 4
            Pseudo Genes (internal stop)      :: 1 of 4
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2296
                     /organism="Candidatus Zixiibacteriota bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_459132"
                     /isolate="SMAG_U2949"
                     /isolation_source="soil"
                     /db_xref="taxon:2053527"
                     /environmental_sample
                     /country="USA: Oregon"
                     /lat_lon="42.99 N 123.63 W"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            380..1246
                     /locus_tag="VGB16_02740"
     CDS             380..1246
                     /locus_tag="VGB16_02740"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_014209729.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="SDR family oxidoreductase"
                     /protein_id="HEX9916629.1"
                     /translation="MESNKAIPIFGGATAIVTGGASGIGRALAEELSKCDCEVVLADL
                     QIELAEEVASEIHTSGGKATAIKIDVTDFSAMEQLAQETVSRTGRLDFIFNNAGIVIG
                     GLVNRLGIEDWNQIVDINLRGVINGIQAAYPIMMKQGFGHIINTASMAGLMVGPGNVA
                     YTTTKYAVVGLSKSLRAEAAQFGIRVSVLCPGVIRTPILEGGGKYGKLLIDIPPEELR
                     RMWEKLRPMSPDRFAKEVLKSVAKNKAIIIVPSWWKLFWWINRLSPSLGILLAQKHFQ
                     YRQKMFGILQKS"
     gene            1983..2258
                     /locus_tag="VGB16_02745"
     CDS             1983..2258
                     /locus_tag="VGB16_02745"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_020886703.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0110001 - toxin-antitoxin complex
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="type II toxin-antitoxin system Phd/YefM family
                     antitoxin"
                     /protein_id="HEX9916630.1"
                     /translation="MKLSSQIKPISYLKAHAAEIVRKLNEHREPMVITQNGEAKVVIQ
                     DIESYEQTQETMALLKILALGTRQIGEGKVQPAEDVIKRLRERQEAH"
BASE COUNT          645 a          499 c          585 g          567 t
ORIGIN      
        1 ttgacataca agaccgttct tgcgaacctg tgggggtcaa agcttcaaaa ttcgattgag
       61 aaggatttga atattgcgtg gtcaaaagag taaaagagat tgggagatct tttgggataa
      121 atggatcagc agtgagcgaa gtcatgacgg gaataaaggt aaaggggcct ggggtcagac
      181 ttgaatattg acatctaagg acaaaaagag agatcgtctt gtctttgacg agagtaagga
      241 aaaatctcaa taatttagcc taaccctgaa gaattgagtt ctattttgac ccagcagaag
      301 aagcggtttt taaaaaagtc actttttcta cagactcgtt ttgaaaggat tcagtattac
      361 tttttagcga gaaatgacta tggaatcaaa taaagcaatt cctatttttg gtggtgccac
      421 tgcgattgtc actggcggag catcaggaat tggccgagcc cttgccgagg aattgtcaaa
      481 gtgcgactgc gaggtcgtat tggccgatct ccagatcgaa ttagcagaag aagttgcttc
      541 agaaatccac acttccggtg gcaaagcaac ggcaataaaa atagatgtga ccgatttttc
      601 tgcgatggaa cagctcgccc aagaaacggt ttcccgtacg ggtcggttag actttatttt
      661 caacaatgcg ggaattgtaa ttggtggcct cgtgaaccga ttgggtattg aagattggaa
      721 tcaaattgtg gatattaacc tccgtggtgt gatcaatgga atccaagcag catacccaat
      781 tatgatgaag cagggattcg gacatattat caatactgcc tcaatggctg gacttatggt
      841 cggtccagga aatgtagctt atacgacgac caagtatgcg gtagttggtc tttcaaaatc
      901 gctacgggcc gaagccgcac agtttgggat tcgtgtgagt gtcttatgcc ctggcgttat
      961 acgtacccct attttggaag gaggggggaa gtacggcaaa ttgcttatag atattcctcc
     1021 tgaagaactc cggcgaatgt gggaaaaact caggcctatg tccccagatc gatttgcaaa
     1081 agaggtgttg aaatcagttg caaagaataa ggcaatcatt atcgtgccgt catggtggaa
     1141 gctgttctgg tggatcaatc gtttgtctcc ttcacttgga atactccttg cgcaaaagca
     1201 ttttcaatat aggcaaaaga tgtttggcat attacaaaag agctaacact tcattccagc
     1261 gcatggctca cagccaccgc tgacctaggc gttgaggctg tagaaaaagt ccacaagcaa
     1321 attcttgggc gagatgcaga aaaagtgacc tcgtagaatg cgccacaatc aacgatctta
     1381 tactggggca ctgctgtcca agaacggtgg aatgaggtgt aatggtagat gatttcattg
     1441 gactttttag ctagtatacc cctctttcaa aatcaggttt accgcagagt cgcggaggtc
     1501 gctgagaagg atctttcttc tggtccaatc ccgcgtttcg cgggattgga ccagaaactg
     1561 ccccccttcg accataaaac cgggactctt tgaaggctat acaagctatt cagggccctt
     1621 cccagagtaa cagaagtctt tttatttggc ggcctctcac cgccaaataa aaaatcggtt
     1681 tctctgcgtc ctttgcgtct ctgcgttgag aatcctactt tggacaagag tgatactggg
     1741 gaagggtcag gtgaccgtcg aagacacccc gttcccactg atcaaaaaga cttttcgtac
     1801 aggctcgtta ggcatgaaca aacagcgaca ttctcaggtg tgagttgaac aggcagaaag
     1861 tatctgccgg gcatgaaaac gaaaatcacc ggacaccgca tcgagcaccc cccccctcct
     1921 ttttgacagt tgacctgttt tcggactata tttagtcgtg tttaagaaat ggaggtgctc
     1981 ccatgaaatt gtccagtcag atcaagccca tcagttacct gaaggcccat gccgccgaga
     2041 ttgtaagaaa gctgaacgaa caccgggagc cgatggtcat cacccagaac ggcgaagcca
     2101 aagtggtcat acaggacatc gaaagctacg agcaaaccca ggaaacaatg gccctcctaa
     2161 agatcctggc gctcgggacc cgtcagatcg gggaagggaa ggtccagccc gctgaggatg
     2221 tgatcaaacg tctgcgcgag cgacaggagg ctcactgatg ccgttcgcgg ttttcctgac
     2281 caacgatgcc gcgggt
//
LOCUS       DASWLN010000083         8136 bp    DNA     linear   ENV 20-FEB-2024
DEFINITION  MAG TPA_asm: Candidatus Zixiibacteriota bacterium isolate
            SMAG_U2949 k141_76647, whole genome shotgun sequence.
ACCESSION   DASWLN010000083 DASWLN010000000
VERSION     DASWLN010000083.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37960594
            Sequence Read Archive: SRR10376091
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Candidatus Zixiibacteriota bacterium (soil metagenome)
  ORGANISM  Candidatus Zixiibacteriota bacterium
            Bacteria.
REFERENCE   1  (bases 1 to 8136)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 8136)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (26-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/12/2024 08:28:26
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 1,440
            CDSs (total)                      :: 1,416
            Genes (coding)                    :: 1,412
            CDSs (with protein)               :: 1,412
            Genes (RNA)                       :: 24
            rRNAs                             :: 1 (16S)
            partial rRNAs                     :: 1 (16S)
            tRNAs                             :: 21
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 4
            CDSs (without protein)            :: 4
            Pseudo Genes (ambiguous residues) :: 0 of 4
            Pseudo Genes (frameshifted)       :: 0 of 4
            Pseudo Genes (incomplete)         :: 3 of 4
            Pseudo Genes (internal stop)      :: 1 of 4
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..8136
                     /organism="Candidatus Zixiibacteriota bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_76647"
                     /isolate="SMAG_U2949"
                     /isolation_source="soil"
                     /db_xref="taxon:2053527"
                     /environmental_sample
                     /country="USA: Oregon"
                     /lat_lon="42.99 N 123.63 W"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            <1..990
                     /locus_tag="VGB16_02750"
     CDS             <1..990
                     /locus_tag="VGB16_02750"
                     /inference="COORDINATES: protein motif:HMM:NF045041.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEX9916631.1"
                     /translation="KFQIILLILAGVILLFISCAEEVINPPEGKVTYAIADYFPLQIG
                     DEWTWQVHSRDSIPEPYKDGDSCVGEPFTDVDSDGIYTPGIDIFVVCGTPNEPCSANQ
                     DLNYNGGYDGRDMCGQVDNVPFVDFDGNGAFDFPDGQYDPGEPYLDLSGNGIRDYTND
                     FDVKMKTSHTEHSVYDYTTYKVLLSMYDTVGLVVYRVNYRNGFSVDGLGLRWHSHYNF
                     ANHYDVLQYGNVRPVTIAAESLEVGYSHTQADTFWADTLVWSSSLVGVEDMTVPAGTF
                     ENCLKFEFVASGWEESMAKFNGTSYWWLAKDVGLVKVQEPDSDWWVLKEYSVE"
     gene            1198..2631
                     /gene="glnA"
                     /locus_tag="VGB16_02755"
     CDS             1198..2631
                     /gene="glnA"
                     /locus_tag="VGB16_02755"
                     /EC_number="6.3.1.2"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011641180.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004356 - glutamine synthetase activity
                     [Evidence IEA];
                     GO_process: GO:0006542 - glutamine biosynthetic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="type I glutamate--ammonia ligase"
                     /protein_id="HEX9916632.1"
                     /translation="MFKSPNEAIKFAKAKKIEMIDLKFIDLFGRWHHLSLPKSQLNMD
                     MFERGVPFDGSSTPGFKSVEAGDMVLIPDIATAKLDPFWDMPTLSFICSSAEADTLEL
                     YRKEPRSIAQKAERYLVQTGIADKSLWSTEFEFYIFDNVSYKNDINMASYIIDSNEAD
                     WNSGLREGKNLGHKIPSHSGYHAIPPLDDLFNLRAQMVKAIEHGGIPVRYHHHEVGGP
                     GQSEIEILDDTLTNMGDITMWVKYVIKMMARKHDRTVTFMPKPLYNEAGNGMHFHQHL
                     FKKAKPLFYDRKGYAGLSQLALYYIGGLLAHGGALVAFTNPSTNSFKRLVPGFEAPVK
                     LFFSLANRSAAIRIPKHAQRPEEKRIEFRPPDASCNVYLALAAQLMAGIDGIKRKIDP
                     TKAGFGPFDKNIFALSEKEKGSMKSVPSSLKEAMEALREDHQFLLEGGVFTREILDVW
                     FNYKLNKEYNEVRNRPHPYEISLYYDV"
     gene            2717..4045
                     /locus_tag="VGB16_02760"
     CDS             2717..4045
                     /locus_tag="VGB16_02760"
                     /EC_number="6.3.1.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_012545065.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004356 - glutamine synthetase activity
                     [Evidence IEA];
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_process: GO:0006542 - glutamine biosynthetic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="glutamine synthetase family protein"
                     /protein_id="HEX9916633.1"
                     /translation="MTKSKEEILKLIDENKVKYIRLWFTDILGQLKGMSITRSEIEHV
                     LEEGQGFDGSSVEGFVRIEESDLVAMPDLKTFRILPWSINDEKVGLVICDVLNPDGTP
                     FVGDPRYVLRKTLDKIAKKGWTFYCGPEIEYFYFAGKDNPTPLDQGGYFDYSTVSVGT
                     RMRKTAASALETMGIPVECTHHEVAPSQHEIDLRYQEALVMADFVMFYRLTIKELAFR
                     EGYHATFMPKPMFGENGSGMHTHQSIFENNRNTFFDPKEEKYHLSKEAKHYIGGVFRH
                     AKEICLVLSQWVNSYKRLVPGYEAPAYICWGTKNRSALIRIPEYKPGKEKATRIELRS
                     PDPACNPYLAFALMLAAGLKGIEEKIEPPAPVEKDIFGLSDSERKKLNIDCLPGSLEE
                     AIGEFEKSKLAKEVLGEHIFYSLIANKKEEWDNYRIHVSDYELEKYLPWL"
     gene            4171..4809
                     /locus_tag="VGB16_02765"
     CDS             4171..4809
                     /locus_tag="VGB16_02765"
                     /inference="COORDINATES: protein motif:HMM:NF024880.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="methyltransferase domain-containing protein"
                     /protein_id="HEX9916634.1"
                     /translation="MANILKQNRLFWERCFSNSGFDFKLPPKRRISEVVKRLRKYNVS
                     KVLDLGCGFGRWSIALARAGFQVKAVDISSEAIKRLTKWANQKSLSIETKVSSAQRLV
                     SIGEKVDAVICNSVLDHMLSSEASKSMLNLKNALKFGGIAYLSFDGLDQEDKDKHVSL
                     EDSTRRYTEGKLKGMLWRFYSNEEIRSLCKDMEVLEFIENSNGKREVWIRKK"
     gene            5016..5651
                     /gene="purN"
                     /locus_tag="VGB16_02770"
     CDS             5016..5651
                     /gene="purN"
                     /locus_tag="VGB16_02770"
                     /EC_number="2.1.2.2"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_007789646.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004644 - phosphoribosylglycinamide
                     formyltransferase activity [Evidence IEA];
                     GO_process: GO:0009152 - purine ribonucleotide
                     biosynthetic process [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="phosphoribosylglycinamide formyltransferase"
                     /protein_id="HEX9916635.1"
                     /translation="MKKLKLLVLASGGGTNLQAIIDSIEQGKLNAEIKVVVSNNSNAF
                     ALERARQHNIKAIHLSHKMFTTPEEFDAKLLSILKENEVNMIILAGYMKMLSPTVVRV
                     YKNRIINIHPALLPSFGGPGMYGIHVHEAVINSGAKTTGVTVHLVDEVYDHGAIVLQE
                     AIPVLDSDTPETLQQRVLEIEHKLYSQAIQLFAEDRIEISENRSFIKKNPK"
     gene            5648..7135
                     /locus_tag="VGB16_02775"
     CDS             5648..7135
                     /locus_tag="VGB16_02775"
                     /inference="COORDINATES: protein motif:HMM:NF024629.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="glycosyltransferase family 39 protein"
                     /protein_id="HEX9916636.1"
                     /translation="MITSQGNRNYFKYLIYLILGTTLFRLIYINLPGLAPQEAYYWNY
                     SRHLALSYFDHPPILAYLIFIFTHLGKQSAFFVRIGCVLLTSGLTYLTFAIGKLLFDE
                     RIGFFSAVLLNSVLIFSLGAMIATPDTPMIFFWALSFYFFCKLILTQDKKWWYVWGIS
                     TGLAMLSKYSSVFILVSVSLFLVFSRENRKWLLCKEFYLALLLAVLVFSPVIIWNAHN
                     NWASFLFQSSRRAKEISSFSARNFFGYLGAQIGIVSPLVYISLIYSTVKSGIIGFKEN
                     NQKFSLCFFWSFPIILFFTLVATKYWVKMNWVCAGYYSASIAGVALIFWFWEKGAKWV
                     RSWGISALTLSLIFVVLGHILPVMKTIPLSPSLDTITGWKELAQRVESEKSKMDEKTF
                     VVGYGYKVASEIAFYTHPQLETYSNNIVGESGLQFDFWSKPDSFLGRDAIFVYDQRER
                     YKNPENLKDYFVNVEELEPLRIYRGGRVLTTFSIFKCYEYKGLGS"
     gene            7110..7685
                     /locus_tag="VGB16_02780"
     CDS             7110..7685
                     /locus_tag="VGB16_02780"
                     /inference="COORDINATES: protein motif:HMM:NF016060.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0016020 - membrane [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="GtrA family protein"
                     /protein_id="HEX9916637.1"
                     /translation="MNIRVLAVKIVKEFEVSRFLKFCLVGASGVLVNMFFFWLFYKKL
                     GIFSLFSSFLAIQIAILNNFLWNDKWTWREKRKPGTGEFFARLGKFTLSSNLTSASAN
                     LLGVWIFLNLLGWNYLTSNLLGIGLGVILNFLANHYWTYSPPPEGPRRRMLASKRRSS
                     TFGGNGEASLSALASRRSRGKPRAFYILEAP"
     gene            7971..>8136
                     /locus_tag="VGB16_02785"
     CDS             7971..>8136
                     /locus_tag="VGB16_02785"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_012545457.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="phosphoribosylaminoimidazolesuccinocarboxamide
                     synthase"
                     /protein_id="HEX9916638.1"
                     /translation="MEKAILETHIKEFQLFSKGKVRDVYDLEDRLLIVATDRISAFDV
                     VLPTGIPGKGK"
BASE COUNT         2353 a         1550 c         1924 g         2309 t
ORIGIN      
        1 aaattccaaa taatcttatt gatcctggct ggcgtaattc ttctattcat cagttgtgcg
       61 gaggaggtaa ttaatcctcc agagggaaaa gtcacttacg ccattgccga ttattttcct
      121 ctgcaaattg gggatgaatg gacttggcaa gtacacagta gagacagtat tccagaacca
      181 tataaagatg gggatagttg tgttggagag ccgtttacag atgtggatag cgatggcatc
      241 tatactcccg ggatagatat ttttgtagtc tgcggaaccc cgaatgaacc ttgctcggcg
      301 aatcaggatt taaattataa tggtggttat gatggacgag atatgtgtgg tcaagtagat
      361 aatgttcctt ttgtcgattt tgacggcaac ggcgctttcg attttcctga tggtcaatat
      421 gatcctggag aaccatatct tgatttgagt gggaatggga taagggatta cacaaatgat
      481 ttcgatgtca aaatgaaaac aagtcatacc gaacattctg tctacgatta cacgacctat
      541 aaggtattgc ttagcatgta tgatacggtc ggattggtag tctatcgggt gaattataga
      601 aatggattta gcgtagacgg tctcggtttg aggtggcatt ctcattataa ttttgcaaat
      661 cattatgatg tactgcaata cggaaatgtg aggcctgtaa ctattgcagc tgagagttta
      721 gaggttggtt atagccacac acaggcagat actttctggg cggatactct tgtttggagt
      781 tcttctctgg ttggagtcga agacatgact gttcctgcag gaacatttga aaattgtctc
      841 aaattcgaat ttgttgcttc tgggtgggaa gagagtatgg caaaattcaa cggcacaagt
      901 tactggtggt tggcaaaaga tgtgggcttg gttaaagtgc aggaacctga cagtgattgg
      961 tgggtgttga aagagtatag cgtggaatag atatttgtaa gtctaaacaa taattagagt
     1021 tacgataata gcagtggtta tttgtcactg ctatttcatt tactgtcgtg agcaatttaa
     1081 agtcgtacat ttaaaaattt ttgagcttct attttggttg actttctgtt ttgtttatat
     1141 taattttaaa ttttcctaaa tcaatgaatt atttgaattc ttgaaatgga ggcaaagttg
     1201 ttcaaaagcc caaatgaagc aattaagttt gcaaaagcaa agaagatcga aatgatagac
     1261 ctaaaattca tcgacctttt tggaagatgg catcatcttt ccttgcccaa aagccaatta
     1321 aatatggata tgtttgaaag gggtgtcccc tttgatggct cttcgacacc tggatttaag
     1381 tcggtggaag cgggcgatat ggttttaatt cccgatattg cgaccgctaa attagaccca
     1441 ttctgggaca tgcctacttt gagcttcatc tgtagttcgg cagaggcaga caccctggag
     1501 ctatacagaa aagagccgag gtctattgcg cagaaggctg aaagatacct agttcaaaca
     1561 gggattgcgg acaagagtct ttggtcaaca gagtttgagt tttacatatt tgacaacgtg
     1621 agctataaga acgatatcaa catggcttcc tacattatag attctaatga ggcagactgg
     1681 aactccggat taagagaagg aaaaaacttg ggccataaaa tccccagcca tagtggatat
     1741 catgccatcc ctccattgga cgatcttttt aatctgcgcg cacaaatggt caaggccata
     1801 gagcacgggg ggataccggt aagatatcat catcatgagg tgggaggtcc aggacaatcg
     1861 gagatagaga tactggatga tactcttacc aacatgggag acataaccat gtgggtcaag
     1921 tatgtgatca agatgatggc aagaaaacac gaccgtaccg ttaccttcat gcctaagccg
     1981 ttgtacaatg aagcaggaaa cggcatgcat tttcaccagc atcttttcaa gaaagccaaa
     2041 ccgctatttt atgacaggaa aggatatgcc ggtttaagcc agctggcttt gtactatata
     2101 ggcggccttt tggctcacgg aggcgccctg gtggctttca ccaaccccag caccaactcc
     2161 ttcaaaagac tagttcccgg atttgaggcg ccggtaaaac tatttttctc tttagctaat
     2221 cggagcgcgg ctattcgcat tcccaaacat gcccagaggc cggaagagaa aagaatagag
     2281 tttcgacctc cggatgcaag ctgcaacgtc tatttagctt tggcggcaca gcttatggca
     2341 ggaatagacg ggatcaaaag gaagatcgat cccaccaaag caggatttgg cccattcgac
     2401 aagaacattt ttgctctaag tgagaaggaa aaagggagca tgaaatccgt tcccagttct
     2461 ttgaaggaag caatggaagc tttaagggag gatcaccaat ttctgcttga aggaggggtc
     2521 ttcacccgag agatccttga cgtatggttc aattataagt tgaacaagga atataatgaa
     2581 gtaagaaata gaccccatcc ttacgagata tctctttatt atgatgtatg aattttaagc
     2641 gcaagaaaat aggagtcaag tttcacgggt gaagttgttc tatcacctag aagtcattca
     2701 aaagaggcaa agggccatga ccaaaagcaa agaggagatt ttgaaattga tcgatgagaa
     2761 taaggtaaag tatattcgct tgtggttcac tgatatttta ggtcaactca aggggatgtc
     2821 catcactcgc agcgagatag agcatgtgct ggaagaaggt cagggatttg acgggtcttc
     2881 agtggaaggg tttgtccgca tcgaggaaag cgaccttgtg gctatgcctg atttgaagac
     2941 ttttcgcatt ttgccctggt cgattaacga tgagaaggtg ggactggtaa tctgcgatgt
     3001 tttaaatcca gacggaacgc cttttgttgg tgacccgcgc tatgtgttgc gtaagacttt
     3061 ggataagatt gccaaaaaag gatggacttt ctattgtggt ccggaaatcg aatattttta
     3121 ctttgctgga aaagataatc caactccttt ggaccaaggg ggatatttcg attacagtac
     3181 tgtaagtgta ggaaccagga tgcgaaagac agctgcctca gctctagaaa caatgggaat
     3241 tccagtagag tgtactcatc atgaggtcgc accatctcag cacgagatag accttcgcta
     3301 tcaagaagcc ttagttatgg ctgatttcgt catgttctac cgcctcacca taaaagagct
     3361 tgctttccga gaaggttacc atgccacctt catgcctaag ccgatgtttg gtgaaaatgg
     3421 ttccggcatg catactcatc aatccatatt cgagaacaac agaaacacgt tctttgaccc
     3481 aaaggaggag aaataccatc tctccaaaga ggcaaagcac tatattggtg gagtttttcg
     3541 ccatgccaaa gagatatgct tagttttgag ccaatgggtc aactcctata agcgacttgt
     3601 gccaggatac gaagcgccgg cctacatctg ttggggaacg aaaaatcgct cagctttgat
     3661 tcgcataccg gaatacaaac ctggaaaaga gaaagccact cgtattgaac tacgttctcc
     3721 ggacccggcc tgtaatccct atttggcttt tgctctgatg ttagccgccg gtttaaaagg
     3781 aatagaggag aagattgagc cgccggcccc agtggaaaaa gacattttcg gactgtctga
     3841 tagcgaaagg aaaaaactga atattgactg ccttcccggt tcgttggaag aagccatagg
     3901 agagttcgaa aaaagtaagt tagcaaaaga ggttttaggt gagcatattt tttattcttt
     3961 aattgctaac aagaaagagg agtgggacaa ctatcgcatt cacgttagcg attacgagtt
     4021 agagaaatat ctgccttggc tatagattat cgtcttgctt ttttgtcaag agaactgtag
     4081 cggtctgatt tatcaagcat tatgtagcaa tcggttacaa aatccgactg gatagactct
     4141 gttaggaatt gctcctgaca taactgaaag atggctaata tcttaaaaca aaatagactt
     4201 ttttgggaaa ggtgtttctc taattctggt ttcgatttca agttgccacc gaagaggaga
     4261 atttcagagg tcgtcaaacg actacgaaaa tataatgtat ccaaagtatt agatttaggt
     4321 tgtggtttcg gtcgatggag catagcgctt gctcgggctg gttttcaagt gaaggccgtt
     4381 gatatttctt cagaagcgat aaaaagactt acaaaatggg caaaccaaaa gagtctttcc
     4441 atagagacca aagtatcctc tgctcaaagg cttgtctcca ttggggaaaa agtggacgca
     4501 gtcatctgta attcggtttt agaccatatg ctatcgtctg aggcgtctaa gtcgatgcta
     4561 aaccttaaga atgctttgaa atttgggggt atcgcttatc tctcatttga tgggttagac
     4621 caggaagaca aggacaagca cgtgtcatta gaggatagta caagaagata cacagaagga
     4681 aagctcaaag gaatgctatg gagattctat tctaacgagg agatcagaag cctatgcaaa
     4741 gatatggagg tgctggaatt catagaaaac agcaacggaa agcgagaggt gtggattcgt
     4801 aaaaagtgac gaactctttt ttgtgctcgg cagtttccct gacccaacga gaaaagaagt
     4861 ctatggagtg catcatccgt gatgatgcat gcattgacac ggtcaatgca ctccaaaata
     4921 agattcctgt caggcacgga ataataaccc ggggctgtag cagttcgatt tatcgaacaa
     4981 aattcaaaaa agataattga gggagcaagc cacagatgaa aaaactaaaa cttttagtct
     5041 tggcttcggg tggggggacg aatctgcaag ccataatcga ctcaattgag caaggtaaat
     5101 taaatgcaga gataaaagtg gtggtgagca acaacagcaa cgcatttgct ttggagcggg
     5161 caagacagca taatatcaag gcgattcatt tgagccataa aatgtttacc accccagaag
     5221 agttcgatgc aaaacttctt tccatactga aggaaaatga agttaatatg ataattttgg
     5281 cgggatatat gaagatgctt tcgccgaccg ttgttagggt atataaaaac agaatcataa
     5341 atattcatcc agccctgtta ccttcttttg gcggaccagg gatgtatggc attcacgtcc
     5401 acgaagcggt gataaattca ggtgcgaaga caaccggggt cacagttcat ctggtggacg
     5461 aggtttatga ccatggagca atagttcttc aagaggcgat tccggtgttg gatagtgaca
     5521 ctccggaaac cctacaacaa agagttttgg aaattgaaca caagctttat tcccaagcca
     5581 tccagctttt cgcagaggat agaattgaaa tctcggaaaa tcgaagtttc atcaagaaaa
     5641 accccaaatg atcacttctc agggaaatcg aaattatttc aaatatctta tttatctcat
     5701 attaggcaca accctttttc ggctgattta catcaacctt ccgggcttag caccccagga
     5761 agcatattac tggaattact cccgacattt ggctttgagc tatttcgacc atccacccat
     5821 actggcctat ctaatcttca tcttcaccca tctcggaaag cagagcgcat ttttcgtgcg
     5881 cataggctgt gtgctactga cttccggttt aacctatctg acctttgcca ttgggaaatt
     5941 actttttgac gaaaggatcg gctttttttc ggctgtactt ctgaacagcg ttttgatttt
     6001 ctccctggga gcaatgatcg ccactcctga taccccgatg attttcttct gggcactttc
     6061 cttttacttt ttttgcaaac taattttgac tcaagataaa aagtggtggt atgtttgggg
     6121 aataagcacc ggattagcta tgctttcaaa gtatagttca gttttcattc ttgtgtctgt
     6181 ttcccttttt ttagtcttct cccgagaaaa ccggaaatgg ttactttgca aggagtttta
     6241 tttagctctg cttttagcag tcttggtctt ctccccggtc atcatttgga atgcccacaa
     6301 taattgggct tcctttttgt ttcaatcctc gcgccgagcc aaagagataa gttccttttc
     6361 agcccgtaat ttctttggtt atctgggagc ccaaattgga atagtctcgc ctctggttta
     6421 catcagttta atctactcaa ctgtcaaaag tggaatcatc ggatttaaag agaataatca
     6481 gaagttttcg ctttgctttt tttggtcttt tccaataatt ttgtttttca ctttagttgc
     6541 gaccaaatat tgggtcaaaa tgaactgggt gtgcgcagga tattactccg cttcgattgc
     6601 aggcgtggct ttaatttttt ggttttggga gaagggtgca aaatgggtta gaagttgggg
     6661 aatttcagct ttaactctca gcttgatttt cgttgtgctt ggccatattt tacctgtgat
     6721 gaaaaccatc ccgctttcgc cttcgttaga tactataact ggctggaaag aattagccca
     6781 gagagtggaa agtgaaaaaa gtaaaatgga tgaaaagaca tttgtggtgg gttatggtta
     6841 caaagtggct agcgagattg cgttttacac ccatccccaa ttggaaacat attccaacaa
     6901 cattgtaggt gagagcggac tgcagtttga tttctggtcg aaaccagata gttttttggg
     6961 aagggatgct atttttgtgt atgaccaaag ggaaagatat aaaaaccccg aaaacttaaa
     7021 agactacttc gtaaatgtag aagagttaga acctctacga atttatcgag gaggaagggt
     7081 tctaacgact ttcagcatct tcaaatgcta tgaatataag ggtcttggca gttaagatag
     7141 taaaagaatt tgaagtgagc cgttttttaa aattctgctt ggtcggcgcc agcggagttt
     7201 tagtcaatat gtttttcttc tggctctttt acaaaaagct gggaattttc tcactttttt
     7261 ccagctttct ggcaatccag atagccattt tgaacaattt cctctggaac gataaatgga
     7321 cctggagaga aaagagaaaa ccgggaacag gggagttttt tgcaaggttg ggtaaattca
     7381 ccctctcttc taatcttacc tccgcttctg ccaatctttt gggggtgtgg attttcctga
     7441 accttttggg atggaattat ctgacttcga atcttttggg cataggttta ggtgttattt
     7501 tgaatttctt agcaaaccac tactggactt actcgcctcc gccagaaggc ccccgtagga
     7561 ggatgcttgc ctcaaagagg cgaagttcca cctttggcgg aaatggcgag gcgagcctct
     7621 cggcgctcgc ctctcggcga agccgaggga agccgagggc cttttatatt ttggaagcac
     7681 cgtgatttat cacggggagg cttcactttt ctggtgacaa aaccaatacg aacgcattta
     7741 ttcgggttca atggaatgcg gagcattcat acgaatctcc cctagccctc gcctcgattc
     7801 ggcccgcctc ggcgagccat cggcgagacg ggcgaagcgg gcctcttttc taaagagggg
     7861 aaaagagatc tccccctttg aaaaaggggg aataaggggg attttgaaag aattacacaa
     7921 tccctgccgt gtaacagtaa ttaatgcgtt tgctataatt aaaggagatt atggaaaaag
     7981 ctatattaga aactcatatc aaggaatttc agctgttttc aaaaggaaaa gtgcgggatg
     8041 tatatgattt agaagataga ctcttaattg tcgctacgga tagaatctca gccttcgacg
     8101 tggttctccc caccggaatt cccggcaagg gaaaga
//
LOCUS       DASWLN010000084         3556 bp    DNA     linear   ENV 20-FEB-2024
DEFINITION  MAG TPA_asm: Candidatus Zixiibacteriota bacterium isolate
            SMAG_U2949 k141_967683, whole genome shotgun sequence.
ACCESSION   DASWLN010000084 DASWLN010000000
VERSION     DASWLN010000084.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37960594
            Sequence Read Archive: SRR10376091
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Candidatus Zixiibacteriota bacterium (soil metagenome)
  ORGANISM  Candidatus Zixiibacteriota bacterium
            Bacteria.
REFERENCE   1  (bases 1 to 3556)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 3556)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (26-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/12/2024 08:28:26
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 1,440
            CDSs (total)                      :: 1,416
            Genes (coding)                    :: 1,412
            CDSs (with protein)               :: 1,412
            Genes (RNA)                       :: 24
            rRNAs                             :: 1 (16S)
            partial rRNAs                     :: 1 (16S)
            tRNAs                             :: 21
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 4
            CDSs (without protein)            :: 4
            Pseudo Genes (ambiguous residues) :: 0 of 4
            Pseudo Genes (frameshifted)       :: 0 of 4
            Pseudo Genes (incomplete)         :: 3 of 4
            Pseudo Genes (internal stop)      :: 1 of 4
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..3556
                     /organism="Candidatus Zixiibacteriota bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_967683"
                     /isolate="SMAG_U2949"
                     /isolation_source="soil"
                     /db_xref="taxon:2053527"
                     /environmental_sample
                     /country="USA: Oregon"
                     /lat_lon="42.99 N 123.63 W"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            <1..474
                     /locus_tag="VGB16_02790"
     CDS             <1..474
                     /locus_tag="VGB16_02790"
                     /inference="COORDINATES: protein motif:HMM:NF014385.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0015562 - efflux transmembrane transporter
                     activity [Evidence IEA];
                     GO_process: GO:0055085 - transmembrane transport [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="TolC family protein"
                     /protein_id="HEX9916639.1"
                     /translation="NWLPTVSLSYRYGWSNENLDMLGYFLKKDYDQTISGSLNFNIFN
                     GFQNKAATSNAKLDARYAQENYRQTKENVALEVKDAYLTIQQAKEKLDLTGESVRSAK
                     EDLDLVQEKYNLGAATILELLDAEVSFKQAKSDEVQALFDYNMAVAKLEKAIGKI"
     gene            484..1749
                     /locus_tag="VGB16_02795"
     CDS             484..1749
                     /locus_tag="VGB16_02795"
                     /inference="COORDINATES: protein motif:HMM:TIGR01730.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005215 - transporter activity [Evidence
                     IEA];
                     GO_process: GO:0006810 - transport [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="efflux RND transporter periplasmic adaptor
                     subunit"
                     /protein_id="HEX9916640.1"
                     /translation="MKKRSKLIIILAVVVVVVIIVLANVLKSGEKTFAVEAEKVKKGD
                     VTSVASADGKIDPRTTIKISAYVPAKVTHLAVKEGEAVAKGQLLVELDPTRYQAEVNR
                     MEAQVRSDQASLEEIKVAYQRQKQLFEKGLASQQEYDRAKTNYDVTLAQLEQGKAILD
                     QAKDDLSKTRMTSPINGVVTQLSVEEGEIAITGTMNNPGTVLMVISDLSFIEIKAEVD
                     ETDVGNVEKGQKVKVEIDAYPDTSFNGEVTEIGNTALVSGYGTQEQTTNFLVKVLLLD
                     KVPGIKPGMSASVDITTEEHKNVLNVPIQAVVMRSPKDTLEVVAPTKEKKPKKTASAD
                     TTQSSEPKGKEKEIEGVFVIKDHKAVFIPVQTGIADQQNIEIKSGLKDDDMVITGSYK
                     ILRTLKNRAKVKLQKETGIKGTSEKKEKE"
     gene            1764..2450
                     /locus_tag="VGB16_02800"
     CDS             1764..2450
                     /locus_tag="VGB16_02800"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011524124.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_function: GO:0016887 - ATP hydrolysis activity
                     [Evidence IEA];
                     GO_function: GO:0042626 - ATPase-coupled transmembrane
                     transporter activity [Evidence IEA];
                     GO_function: GO:0140359 - ABC-type transporter activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="ABC transporter ATP-binding protein"
                     /protein_id="HEX9916641.1"
                     /translation="MLIRTEDVWKIYRVGTEEVGALRGVTMQVEKGEYVAIMGPSGSG
                     KSTLMNLIGCLDTPTKGSYYLNDKLVSKMTDDELAHIRNKEIGFVFQTFNLLPRASAL
                     HNVELPLVYNGTESTLRIKKAKEALKLVDLEDRMAHKPNELSGGQRQRVAIARALVNS
                     PSLILADEPTGNLDTKTGAEIMELFGKLHKQGNTIIIVTHEHDVARYADRIIHIRDGG
                     VEREEIVKKN"
     gene            2515..>3556
                     /locus_tag="VGB16_02805"
     CDS             2515..>3556
                     /locus_tag="VGB16_02805"
                     /inference="COORDINATES: protein motif:HMM:NF024116.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ABC transporter permease"
                     /protein_id="HEX9916642.1"
                     /translation="MKFLEGVIIALKSLLANKLRSILTLIGIIIGVMTVIAVTSIIAG
                     MNKYVADQISIMGPDTFLVNKWGMIMTEDEWFEAIKRKSITLEDMKALREGCVACYRV
                     GATVVRSGKVRHGSHYIDEVVITGVTANRAEIVDVKVEQGEGRYISKFEEEHNQPVAF
                     VGWEIRDKLFPLVDPIGKEIKIENRTFTIVGVSQKIGSSLVQNQDNSVHIPITTFFKD
                     FGKHEYGRSRSINIVVKAASFESVDEAMDQTRLILRARRHIPYSKKDDFGMMTSETFM
                     DLYRQFTSAAYLTMIGISSIALLVGGIVIMNIMLVSVTERTREIGIRKALGARRRDVR
                     WQFLVESVTLALV"
BASE COUNT         1168 a          605 c          920 g          863 t
ORIGIN      
        1 aattggttgc cgactgtttc cctaagttat agatatggtt ggagcaatga aaacttggat
       61 atgctcggat attttttgaa aaaagattat gaccagacta tcagcggaag tctgaatttc
      121 aatatcttta atggttttca aaacaaggca gcaaccagca atgccaagct ggatgccaga
      181 tatgcccaag aaaattaccg ccaaaccaaa gagaatgtgg ctttggaagt taaggatgcg
      241 tatctgacta ttcaacaagc aaaggaaaaa ttagacttga ctggagagag cgttaggtct
      301 gctaaagaag atttggattt ggtgcaggag aaatacaatt taggggcggc aactatattg
      361 gagctcctgg acgcagaggt gagtttcaag caggcaaaat cagatgaagt gcaggcgttg
      421 tttgattata atatggcagt ggcaaaatta gaaaaggcta ttggcaaaat ctaaggaggg
      481 tgcatgaaaa aaaggagcaa gttgatcata atccttgcgg tagtcgttgt agtagtgata
      541 atcgttctgg caaatgttct aaaaagtgga gaaaagacat ttgcagttga agcggaaaag
      601 gtcaaaaagg gagatgtcac ctcggtagcc tcggctgacg gcaaaatcga cccgaggacc
      661 acaattaaga tcagcgccta cgtccctgcg aaggtaactc acttagcggt gaaggagggg
      721 gaggcggttg ccaaggggca actcctagta gaattggatc ctactagata tcaagccgag
      781 gttaacagaa tggaagctca ggttaggtca gaccaagcca gcttggaaga gatcaaggtg
      841 gcttatcaga ggcaaaaaca gcttttcgaa aaagggttgg catcccagca ggaatatgat
      901 agggctaaaa ccaattacga tgtgaccttg gctcagcttg aacaaggaaa agccatttta
      961 gatcaagcca aggacgactt gtccaaaacc agaatgacct ctccaataaa tggagtcgta
     1021 actcagctta gtgtggagga aggagaaata gccattactg gaacaatgaa taatcctggc
     1081 actgtactta tggttatatc cgatttaagc ttcatagaga ttaaggcaga ggtggatgag
     1141 acggatgtag gtaatgtcga gaaggggcag aaagtaaaag tggagatcga tgcctatccc
     1201 gacaccagtt tcaatggaga ggtaactgaa ataggaaata ctgccttggt ttcaggatac
     1261 ggtactcaag aacagacgac taatttcctg gtcaaagttt tgcttctgga taaggtgccg
     1321 gggataaaac ctggtatgtc agcctcggtt gatattacca ctgaagaaca taaaaatgtc
     1381 ttgaacgttc ccattcaagc ggtggtgatg cgttctccga aagatacttt agaagtggta
     1441 gctccgacca aggagaagaa acccaaaaag acagcgtccg ccgatacaac tcagagttct
     1501 gaacccaaag gaaaggaaaa agagatagaa ggggtctttg tgataaaaga ccataaagcc
     1561 gtatttattc cggttcagac cggcatcgct gaccagcaaa atattgaaat aaaatcaggc
     1621 ctgaaagatg atgatatggt gataactgga agttataaga ttttgagaac tttgaaaaac
     1681 agggcaaaag tcaagttgca aaaagaaacg ggtatcaaag gcacaagtga aaagaaggaa
     1741 aaagaataag gaggacctgc aaagtgttaa tacgaactga agatgtgtgg aaaatataca
     1801 gagtcggaac agaggaagtg ggagccctga ggggagtgac gatgcaggtt gaaaagggtg
     1861 aatacgtggc tattatgggt ccctctggtt cgggaaaatc aactttgatg aatttaatcg
     1921 gatgtctgga tacgccaact aagggttctt attacctgaa tgataagctg gtgagcaaga
     1981 tgaccgatga cgagctggct cacatcagga ataaagaaat aggatttgtg tttcagacct
     2041 ttaaccttct cccgcgtgct tcagctttgc acaatgtcga gttacctctg gtctataatg
     2101 gaaccgagtc aactttgaga ataaagaaag caaaagaagc tctgaagttg gttgacttag
     2161 aggaccggat ggctcataaa ccaaacgagc tctccggagg acaaagacaa agagtagcaa
     2221 tagcgcgcgc tttggtgaat agcccatctt tgattttagc agacgagcca actggaaatc
     2281 tggacaccaa aaccggtgct gaaataatgg agcttttcgg taagctgcac aaacagggta
     2341 acaccatcat catcgtcacc catgaacatg acgtggcaag gtatgcggat agaataattc
     2401 atattcgtga cggcggagtg gagagggaag aaatagtaaa gaagaattag tctcttgagt
     2461 catacgacct aaatacaacc atagtctcga accacagatt gaaaggataa gtttttgaaa
     2521 ttcctggaag gcgttattat agcattaaag tcgcttttgg cgaacaagct gcgctccatc
     2581 ctaaccctga taggaattat cattggggtg atgaccgtga tcgcagtgac ttctattatt
     2641 gctggaatga acaaatatgt agccgaccag attagcatca tgggtcccga caccttccta
     2701 gtcaataagt ggggcatgat aatgactgaa gatgagtggt ttgaggcaat taaaagaaag
     2761 agcattacat tagaggatat gaaagcattg agggagggtt gcgtagcctg ctatagggtt
     2821 ggagccacgg tggtccgctc tggaaaggta aggcatggaa gtcattatat agatgaggtt
     2881 gtaataaccg gagttactgc taaccgtgct gaaattgtag atgttaaggt tgaacaagga
     2941 gaaggaaggt atatctccaa atttgaagaa gaacataatc aaccggtagc ttttgttgga
     3001 tgggagataa gggataagct gtttcctttg gttgatccta ttgggaagga gataaaaatc
     3061 gaaaacagaa cgtttacaat agtaggagta tctcaaaaaa taggttcgtc tttagtacaa
     3121 aaccaagaca attctgtgca cattcctata actactttct ttaaagactt cggaaagcat
     3181 gaatatggaa gaagtcgtag catcaacata gtggttaaag ctgcgagctt tgaatcagta
     3241 gatgaagcta tggatcaaac caggttaatc ttgagagcaa gaaggcacat tccttacagc
     3301 aagaaagatg attttggtat gatgacctca gagaccttta tggatctgta cagacagttc
     3361 acttctgccg cttatctaac tatgataggc atttcctcta tcgctttgct ggttggtgga
     3421 attgtgatta tgaatattat gttggtttca gttaccgaga gaaccagaga gatagggatt
     3481 cgcaaggccc ttggagcaag gagaagggat gtgcgttggc aattcttggt agaatctgta
     3541 accttggctt tggtgg
//
LOCUS       DASWLN010000085         4967 bp    DNA     linear   ENV 20-FEB-2024
DEFINITION  MAG TPA_asm: Candidatus Zixiibacteriota bacterium isolate
            SMAG_U2949 k141_167262, whole genome shotgun sequence.
ACCESSION   DASWLN010000085 DASWLN010000000
VERSION     DASWLN010000085.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37960594
            Sequence Read Archive: SRR10376091
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Candidatus Zixiibacteriota bacterium (soil metagenome)
  ORGANISM  Candidatus Zixiibacteriota bacterium
            Bacteria.
REFERENCE   1  (bases 1 to 4967)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 4967)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (26-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/12/2024 08:28:26
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 1,440
            CDSs (total)                      :: 1,416
            Genes (coding)                    :: 1,412
            CDSs (with protein)               :: 1,412
            Genes (RNA)                       :: 24
            rRNAs                             :: 1 (16S)
            partial rRNAs                     :: 1 (16S)
            tRNAs                             :: 21
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 4
            CDSs (without protein)            :: 4
            Pseudo Genes (ambiguous residues) :: 0 of 4
            Pseudo Genes (frameshifted)       :: 0 of 4
            Pseudo Genes (incomplete)         :: 3 of 4
            Pseudo Genes (internal stop)      :: 1 of 4
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..4967
                     /organism="Candidatus Zixiibacteriota bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_167262"
                     /isolate="SMAG_U2949"
                     /isolation_source="soil"
                     /db_xref="taxon:2053527"
                     /environmental_sample
                     /country="USA: Oregon"
                     /lat_lon="42.99 N 123.63 W"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            complement(<1..625)
                     /locus_tag="VGB16_02810"
     CDS             complement(<1..625)
                     /locus_tag="VGB16_02810"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_020251623.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0030170 - pyridoxal phosphate binding
                     [Evidence IEA];
                     GO_process: GO:0009058 - biosynthetic process [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="aminotransferase class I/II-fold pyridoxal
                     phosphate-dependent enzyme"
                     /protein_id="HEX9916643.1"
                     /translation="MKVPLLDLTRQYKSIKNEIDQAISKVLNHSLFILGPEVKSFEEK
                     IAKYCGTKYAIGVASGTDALLLSLRAIGVGQGDEVIVPSFTFYATAGVVTRLGGTPVF
                     VDNDPFTYNIDPELIEKKITKKTKAIIPVHLFGQCVDMDAIMEIARKHNLKVIEDAAQ
                     AISAEYKKRKAGSLGDLGCFSFFPSKNLGGAGDGGMVITNDADLAEKI"
     gene            complement(703..1446)
                     /locus_tag="VGB16_02815"
     CDS             complement(703..1446)
                     /locus_tag="VGB16_02815"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003403318.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DapH/DapD/GlmU-related protein"
                     /protein_id="HEX9916644.1"
                     /translation="MNSHIDKSVKMGEGTKVGINSILDKDVKIGKGCIIGNNVVIHAG
                     TRIGDYVRIDDNTVIGKLPMKAATSATTKEEELSPAEIGDNCIIGTSVVIYAGSKIGK
                     KVLVADLSTVRENVSIGDFTIVGRGVAIENFCKVGSYCKLETNVYITAYSELGDWCFI
                     APCVATSNDNYMARSKERFKHFKGVTVKKGGRIGLNATILPGKIIGEDSQVAAGAVVT
                     RDTPAKKIVLGVPAKPWRDVPPEQLLENQ"
     gene            complement(1464..2474)
                     /locus_tag="VGB16_02820"
     CDS             complement(1464..2474)
                     /locus_tag="VGB16_02820"
                     /inference="COORDINATES: protein motif:HMM:NF014893.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016491 - oxidoreductase activity
                     [Evidence IEA];
                     GO_function: GO:0016651 - oxidoreductase activity, acting
                     on NAD(P)H [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="Gfo/Idh/MocA family oxidoreductase"
                     /protein_id="HEX9916645.1"
                     /translation="MIKTGLIGIGAWGKNLLRNFYGLPNCELVICCDQDEKQLERTQK
                     SYPGMKVTTDPQEVISSNQIQAVVIATPPVSHFDLAKRSILAGKDVFVEKPLVLDVKQ
                     GEELVNLAEKNNRILMVGHIMEYHPASLALKKYIDSGELGKIYYLYATRVNLGKVRDI
                     ENALWSFAPHDISLIIFLLGQSPSSVTATGESYLQKSIEDVAFMTMHFNDGTMAHIHV
                     SWLDPHKDRKLTVVGSKKMAVFDDTESSEKIWIYDKGVDAKSDYDTYGEYLSLRSGDI
                     LIPRIDSSEPLKSECQHFIECVEKRTKPRSDGEDGLRVLRVLDAAQRSLGQGGVPVKI
                     AN"
     gene            complement(2476..3744)
                     /locus_tag="VGB16_02825"
     CDS             complement(2476..3744)
                     /locus_tag="VGB16_02825"
                     /inference="COORDINATES: protein motif:HMM:NF014732.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0016020 - membrane [Evidence IEA];
                     GO_process: GO:0009103 - lipopolysaccharide biosynthetic
                     process [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="Wzz/FepE/Etk N-terminal domain-containing
                     protein"
                     /protein_id="HEX9916646.1"
                     /translation="MKEEEKINIFNYLLVLTKYRKFILLNVVAVCLVVAIVSLFLPSW
                     YAAELTILPPERESLSLGSSLLSSLSGGGMGGGFALPLMATPSDVIASILQSRAVIEP
                     VINQESLIKVYKANSMEEALKEFSSHMKVKVGSEGIISLGFEDKDRNRAARMANSLAL
                     ELDRVNQRTNISKAKNARIFIEERLAQTQKDLAQAEDSLTKFKQENSAISLDDQMKAA
                     INAAAELKAELTKAEIDLNVSSKSLDSSHPEIRRLKTRIAELRNQLATWEFGKGGKQK
                     EGEEKGLLEVPFSEVPSLGLELARLVREVKIQESVFGILTEQYEQYKIQETRDTPTIQ
                     VLDKAVPPQKKSRPRRVFLVGIAGLVSLFSGIFLVFFLEYFEKTRYKNPEDFKKIDLI
                     FNTLQSDFANLKYVFRKRGLGKKEPPAEEK"
     gene            complement(3981..>4967)
                     /locus_tag="VGB16_02830"
     CDS             complement(3981..>4967)
                     /locus_tag="VGB16_02830"
                     /inference="COORDINATES: protein motif:HMM:NF022007.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="SLBB domain-containing protein"
                     /protein_id="HEX9916647.1"
                     /translation="GEVIYVPPTEGQIGICGIYGAVKSPGEFEYSPNDSLLDLIALAQ
                     GLVVNADLAQAEVVRFEEDNKTAQTTKINLKDLILHNQRDKNIPLVPDDRVFIKAIPE
                     YREKKQVRVSGEVVYPGVYPIVEGKDKLTDLVGWAGGFTKDASLLGAEVVRGYNLNIP
                     DPEFERLKKIPVADMSSTEYEYFKAKSQERAGRVACDFRKLFLDKGKNYDVLLKDGDM
                     VNIPAESKMVNISGSIVNPGLVNYLPDQGYGYYIERAGGFSYKARKGKVYIIKGSTGK
                     WVKAKRSTKLDQGDAIWVPEKPDRDYWKFFKDTMLVLGNAATIYLVVKQATE"
BASE COUNT         1276 a         1284 c          880 g         1527 t
ORIGIN      
        1 ctatcttttc agccaaatca gcatcattgg tgatcaccat tccaccatca cctgcaccgc
       61 ctaaattttt gctgggaaag aaactaaaac atcccaaatc ccccaatgaa cctgcttttc
      121 ttttcttgta ttccgcgctg atggcctgag cagcatcctc tataactttc aaattatgtt
      181 ttctggcaat ttccataata gcgtccatgt caacgcattg accaaaaaga tggaccggga
      241 ttatggcttt ggttttttta gttattttct tctcaatcaa ttctgggtca atattatagg
      301 tgaaggggtc attatctaca aaaaccggtg ttcctcccaa tctggtgacc acacccgcgg
      361 tggcgtaaaa ggtgaaagaa gggactatca cctcatctcc ctgaccaact ccgattgccc
      421 gcaaagatag aagcaatgca tctgtaccgg aagccacccc tatggcgtat tttgtcccgc
      481 agtatttggc aattttttcc tcaaaacttt ttacctcagg acctaatatg aacagactat
      541 gatttagaac tttagatatt gcttggtcta tttcattctt gatagattta tattgtcgtg
      601 tcaaatccaa aagtggaact ttcattttct cctcctattt atcatttagg gtcggagcta
      661 ccgaccctaa aatgttttga aaaccgttga agaaattatg gcttactgat tttccagcag
      721 ttgttcgggc ggcacgtctc tccagggttt agccggaact cccagcacta tcttctttgc
      781 cggagtatct cttgttacca cagctccagc cgccacttgc gaatcctcac ctattatttt
      841 gcctggcaga atagtagcgt ttaagccaat ccttccacct tttttaactg taaccccttt
      901 gaaatgtttg aacctctctt tggaacgtgc catataattg tcattgctgg ttgctacaca
      961 aggagcgatg aaacaccagt ctcccaactc cgaataggcg gttatgtaca cattagtttc
     1021 gagtttgcaa taggaaccga ccttgcagaa gttctcaatt gccactcccc ttcccacaat
     1081 ggtgaaatcg ccaattgata cattctctct gactgtggat aaatcagcca ccaaaacttt
     1141 tttaccgatt tttgaaccag catagataac cactgaagtc ccgattatgc agttatctcc
     1201 tatctctgct ggagaaagct cctcctcctt ggttgtagca ctggtagccg ccttcatagg
     1261 aagcttgccg atgaccgtat tatcatctat tcttacgtaa tctccaatcc tcgttccagc
     1321 atggataacc acattattcc cgatgataca tcctttaccg attttgacat ctttatcgag
     1381 aatgctatta attcctactt ttgtcccttc ccccattttg actgatttgt caatgtggct
     1441 attcattcat cttcctccaa taattagttt gctatcttca ctggaacccc accctgccca
     1501 agcgacctct gggccgcatc caagactctc agcactctca atccatcttc tccatcgctt
     1561 ctcggtttgg tccttttctc cacgcactcg ataaaatgct gacattcgct tttcaagggt
     1621 tcgcttgaat ctattctggg aattagaata tccccgcttc ttaagctcaa gtattcaccg
     1681 taggtatcgt aatccgattt ggcatctacc cccttatcgt aaatccatat cttctccgag
     1741 gactcggtat catcgaaaac cgccatcttt ttacttccca caacggtcaa ctttctatct
     1801 ttatgcgggt ccaaccagct cacgtgaata tgagccatag tgccatcatt aaaatgcata
     1861 gtcataaaag ctacatcttc tatacttttt tgaagataag actcgcccgt agcagtaacc
     1921 gaagaggggg actgccccaa aagaaaaatg attaaggaga tatcgtgcgg agcgaagctc
     1981 catagagcat tttcaatatc tctgactttt cccaaattta ccctggttgc ataaagataa
     2041 tagattttac ctaactcgcc tgaatctata tatttcttca aagccaagct ggcaggatgg
     2101 tattccatga tatggcctac cattaagatt ctattgtttt tttcagctaa attaaccagc
     2161 tcctctcctt gcttcacatc cagaaccaaa ggtttctcca caaagacatc cttgcctgcc
     2221 agaatacttc tcttggccaa atcaaaatga gaaaccggag gagtagctat aactactgct
     2281 tgtatttggt tgcttgaaat aacttcctgc ggatcagttg taactttcat gccgggatag
     2341 ctcttttgtg tcctctccag ttgcttttcg tcctggtcgc aacagatgac cagttcacag
     2401 ttgggcaaac catagaaatt cctcaagaga tttttgcccc aagccccaat gccgattaaa
     2461 cctgtcttta tcatattatt tctcttcggc tggtggttct ttcttcccta aacctctttt
     2521 gcggaaaaca tatttcagat tagcaaaatc gctctgcagg gtattgaaaa tcaaatctat
     2581 cttcttgaaa tcctccgggt ttttatatct tgttttctca aaatattcta aaaagaaaac
     2641 caaaaagatt ccactgaaca gactaaccaa acccgctatg cccactaaaa aaacccttct
     2701 gggccgactc ttcttctgtg gaggaacagc tttatccaag acctgaatag tgggagtatc
     2761 tctggtctcc tgaatcttgt actgctcata ttgttccgtc aatatcccaa agacgctctc
     2821 ctgaattttg acctcacgca ccaatcgagc caattccaga cccagagaag gaacttcgga
     2881 aaagggaact tccagtaagc ccttttcctc cccttctttc tgtttacctc cttttccaaa
     2941 ttcccaagtt gccaactgat ttcttagctc agcgattctg gttttaagcc ttctgatctc
     3001 aggatgactg ctatccaagc ttttgcttga gacatttaaa tctatctccg ctttggttaa
     3061 ttccgccttc agctcagccg ccgcatttat cgccgccttc atctgatcat ccagggagat
     3121 agcgctgttc tcctgtttga atttggttag actatcttct gcttgcgcta agtctttctg
     3181 ggtttgagcc agtctttctt cgatgaaaat ccttgcattt ttagctttgg agatattggt
     3241 cctttgattt acccgatcta actctaaagc taatgaattg gccattctgg ctgcccgatt
     3301 cctatctttg tcttcaaatc ccaaggagat gatcccctcg gagccaacct taactttcat
     3361 atgggaagaa aactctttta aagcctcctc catactgttc gccttataaa ccttgattag
     3421 actttcctga tttattactg gctcaatcac cgcacggctc tgcaggatac tggctatcac
     3481 atctgaagga gtagccatta agggaagagc aaaaccacct cccattccac ctccggataa
     3541 gctactgagg agggaggaac ccaaacttaa actctccctt tcgggaggaa gtatggtaag
     3601 ctctgcggca taccagctgg gcaggaaaag acttactatc gccaccacca ggcaaactgc
     3661 cacaacattc aataaaatga acttgcggta tttggtcaga accagaagat aattgaagat
     3721 attgattttc tcctcttctt tcaaacttta ctcctttatg aaaggcatgc cagaataccc
     3781 ccgtaggggg acgaataaca tttctataag ttccctctcc ccttggggga ctgtgtcata
     3841 attcggtttt gtgtagccgt ccacgtggac gaaaacggtt tcttgaattc caagggctta
     3901 cgtccatatg gacggctacg aagtcaaaac atagtcagga ctccccctca agggggagga
     3961 gatgctttgg gcaaggaggt tcactcggtc gcctgtttaa ccactaaata aattgtagcg
     4021 gcatttccca aaactagcat tgtatcctta aaaaatttcc agtaatctct atccggcttt
     4081 tccggaaccc agatagcatc tccttggtcc aatttagttg acctttttgc cttaacccac
     4141 ttgcccgtgc ttcctttgat aatatagact tttcctttcc gcgctttata agaaaaaccg
     4201 ccagccctct ctatgtagta gccataacct tggtctggca agtagttcac cagtcccgga
     4261 ttgactatac tgcccgatat gttaaccatt ttggattcgg cagggatgtt gaccatgtct
     4321 ccatccttca aaaggacatc gtaattcttg cctttatcca agaaaagctt cctaaaatca
     4381 caggccacac gacccgccct ctcctgagat ttagctttaa agtattcata ctcagttgaa
     4441 gacatatctg ctaccgggat cttctttaac ctctcgaatt ctggatccgg aatattaaga
     4501 ttgtatcctc ttacaacttc tgcccctaaa agtgaggcat ccttggtgaa cccaccagcc
     4561 catcccacta agtctgttaa cttatctttt ccctccacta ttggataaac ccctggataa
     4621 accacctccc cactaaccct cacttgtttc ttctcgcgat attctggaat cgccttgata
     4681 aacactctgt catctggcac cagtggtata tttttgtctc tctggttatg caggattaaa
     4741 tccttgaggt taatcttggt agtttgtgcg gttttattat cttcctcaaa ccgcactacc
     4801 tcagcttgtg ccaaatcagc attgactacc aacccctggg ctaatgcaat aaggtccagc
     4861 aggctgtcat taggtgagta ttcaaactcg cctggagatt taactgctcc gtaaatccca
     4921 caaatgccta tttgtccctc agttggcgga acgtagataa cttcacc
//
LOCUS       DASWLN010000086         9405 bp    DNA     linear   ENV 20-FEB-2024
DEFINITION  MAG TPA_asm: Candidatus Zixiibacteriota bacterium isolate
            SMAG_U2949 k141_1051474, whole genome shotgun sequence.
ACCESSION   DASWLN010000086 DASWLN010000000
VERSION     DASWLN010000086.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37960594
            Sequence Read Archive: SRR10376091
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Candidatus Zixiibacteriota bacterium (soil metagenome)
  ORGANISM  Candidatus Zixiibacteriota bacterium
            Bacteria.
REFERENCE   1  (bases 1 to 9405)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 9405)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (26-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/12/2024 08:28:26
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 1,440
            CDSs (total)                      :: 1,416
            Genes (coding)                    :: 1,412
            CDSs (with protein)               :: 1,412
            Genes (RNA)                       :: 24
            rRNAs                             :: 1 (16S)
            partial rRNAs                     :: 1 (16S)
            tRNAs                             :: 21
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 4
            CDSs (without protein)            :: 4
            Pseudo Genes (ambiguous residues) :: 0 of 4
            Pseudo Genes (frameshifted)       :: 0 of 4
            Pseudo Genes (incomplete)         :: 3 of 4
            Pseudo Genes (internal stop)      :: 1 of 4
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..9405
                     /organism="Candidatus Zixiibacteriota bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_1051474"
                     /isolate="SMAG_U2949"
                     /isolation_source="soil"
                     /db_xref="taxon:2053527"
                     /environmental_sample
                     /country="USA: Oregon"
                     /lat_lon="42.99 N 123.63 W"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            <1..271
                     /locus_tag="VGB16_02835"
     CDS             <1..271
                     /locus_tag="VGB16_02835"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=2
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEX9916648.1"
                     /translation="RLGLIIDKMLARKIEDRYKTIDELVSDIEDFEEENRGSQIYLDS
                     PDEHQTDKPIVQTETEKALGRFAPYLFGFLLATAFWLIVYLLLRS"
     gene            746..2665
                     /locus_tag="VGB16_02840"
     CDS             746..2665
                     /locus_tag="VGB16_02840"
                     /inference="COORDINATES: protein motif:HMM:NF025006.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="FG-GAP-like repeat-containing protein"
                     /protein_id="HEX9916649.1"
                     /translation="MLNKLSLIFMFLFSMFVFLTLPAVAQNPDSIPFAPAVNYGAGDK
                     PLSVFCADLDGDADLDLAVANNGSDNVSILKNNGDGTFASAVNYGAGDGAFSVFCADL
                     DRDNDLDLAVANVGSDNVSILNNGNGMFASAVNYGAGDYPSSVFCADLDGDTDLDLAV
                     SNHYSHNVSILKNNGDGTFASAVNYGVGYYPRFVFCADLDGDNDLDLAVANDGNDNIS
                     ILKNNGDGTFASAVNYGAGDGAWSVFCADLDGDNDLDLAVANAWSDNISILFNLTQSI
                     GSISGTVSDSLGGLLGITVDLFDNTGSLVWFAHTDPSGFFSFDSLNAGDYIVSIVTPL
                     GYVADFESRLITVTPNADSVVNFMLHHLEVTSSQRSMGYWKHQVNVYLSGKGSAQESL
                     ANMSKYMSRIKTHFNDNLANPVTIFQVPQPATGRDSLEALQRLLTVNKDGTANDRAKQ
                     QLIALMLNIVSSKLHQTTAVSQDGATASQAVTYCNELITDPDPSNDEIAKDIADNINN
                     GLLISAGIIPTTTPIIVYKYKGGSEGDSEGDLIEASDVPKSFGLLQNYPNPFNLSTKI
                     EFGLKNPGFVSLNIYDVLGRKVRTLVSQDLPSGYKSVLWDGKDDKGLEVASGIYFYRI
                     QAGDFAESKRMLLLK"
     gene            2960..4567
                     /locus_tag="VGB16_02845"
     CDS             2960..4567
                     /locus_tag="VGB16_02845"
                     /inference="COORDINATES: protein motif:HMM:NF013961.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="transglutaminase domain-containing protein"
                     /protein_id="HEX9916650.1"
                     /translation="MHKQDKIILLAVALFIIFPQTVLAVGGVAIAFPSPSPCPQGLAY
                     DGAYLWLVDRKTDMIYKINPKDESIIDSIPSPGYVPRGLTWDGKNLWCVDAEENLIYS
                     INPKTRIVEKTISSPASDPEGLAWDGKYLWIADSKEAKIDQLSTDDGTTITSIPSPSP
                     YPCGLSFDGKYLWVSDRARNMIYMITPDKGEVIVCFDAPGPYTYGLAWDGKYLWNVDY
                     QTDKIYKLKIDDSELFSRKEQKEETLEFTHQIRNYGPDTVKTLDVYLAIPENLNNQEL
                     VGPVEFNPRPTDILTDKWGQKVAHFQFKDLGPTHFNTVSMKAKVKLYQVRYFVFPEKV
                     GKLEDIPKPIKEKYLVDDAKYSMNHPIIQNAVKKAVGDEKNPYWIARKIFSYIIDNME
                     YERVGGWNIAPAVLARGNGSCSEYSFVYIAMCRAAGLPARYVGSVAIRGDDASWDDVF
                     HRWVEVYLPNYGWIPVDPSGGDSKWPADQANAFGYLNNRYLITTTSGGGSEYLDWSYN
                     SNEKWTSEGKCKIVVENIGEWSPLNQF"
     gene            5053..5679
                     /locus_tag="VGB16_02850"
     CDS             5053..5679
                     /locus_tag="VGB16_02850"
                     /inference="COORDINATES: protein motif:HMM:NF015325.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0009055 - electron transfer activity
                     [Evidence IEA];
                     GO_function: GO:0010181 - FMN binding [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="flavodoxin family protein"
                     /protein_id="HEX9916651.1"
                     /translation="MKLLGIVGSPRERGNSFLLMEKVMQSAKKEKPTLLTEIIQLSRQ
                     KIKPCRACQSCADDPYICMQQDDLNEIYKRMKKADGIIFATPSYGPIGACPSVMQAFL
                     ERIANLSHLPSLKNKKFAFPLKDKPCGLIVVSIESQEKALVVLNNLEQYVLAYGMKPV
                     HIKNSSSLGVSGKGNEAGDVLDDKGAVEDSKLLGKLLAKAISPQLRLD"
     gene            complement(6027..7829)
                     /locus_tag="VGB16_02855"
     CDS             complement(6027..7829)
                     /locus_tag="VGB16_02855"
                     /EC_number="5.2.1.8"
                     /inference="COORDINATES: protein motif:HMM:NF025010.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003755 - peptidyl-prolyl cis-trans
                     isomerase activity [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="peptidylprolyl isomerase"
                     /protein_id="HEX9916652.1"
                     /translation="MPVMKGMRDIIKPVMWIVVIAFVGTIVLVWGGDITKSKYKKGII
                     GSANGQEISAADFANLYDNQLKQLGNQSEELDEQTTKQVLDNTWDNLVDEILVQQQTR
                     KLGITVSDKELFEYMKMYPPAEFQQSPDFQIDGKFDYQKYMQALVNPQFPWWRWEGYI
                     RANLITLKLQEMIVDLVRVADPEVKQEYVNDNEKVKVNYLFISNSEFLGKVPPITEQE
                     IQEYYKENQGEFKVESRTSLNFVVIPKVPTQDDIQKTKSDLLRIKMEAEQGEDFATLA
                     QYYSDDKVTAEKGGDLGWFTRGKLIESFEQAAFSLKVGEISQPIRTEYGWHLIKLLEK
                     RGVGKNEELHASHILLKITPTEQTIDSLRVMAEDLVKQATKVGLKEAARQNNLNFSST
                     DFFFRDSIIPEMGYNLGANLFAFENKVGKTSDPIETKNGFYVVEVKDRKPAGIYPLDE
                     VKQKIKDRIVLNKAGDLALGEAQKTYQEAEKTKNLETATSKVNQKIQRLDYFSRSSEL
                     AQVDDNPRFVGTAFSLNEKNKISPPLRTENGIYLIEFVSHIPISEQEFTQAKDSLYQQ
                     LVIQKKTQAYQDWFEQIKKKAKVEDYRNKFYKTE"
     gene            7947..>9405
                     /locus_tag="VGB16_02860"
     CDS             7947..>9405
                     /locus_tag="VGB16_02860"
                     /EC_number="3.4.-.-"
                     /inference="COORDINATES: protein motif:HMM:NF013700.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0046872 - metal ion binding [Evidence
                     IEA];
                     GO_process: GO:0006508 - proteolysis [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="M23 family metallopeptidase"
                     /protein_id="HEX9916653.1"
                     /translation="MLKKIIILLFLITSCSPSLAGDYLWPLKGYDLLSSVFGDYRDFH
                     FHAGIDITTGGKTGAKITAPTDGTVFRVFTSYQGYGKAVYLKAEDGKIFVFGHLSDFS
                     PKLSGLVESEQLKNRRYYIDLLTSGIKIRKGEVIGYSGESGYGGPHLHFEMRDGGNHP
                     MNPLVFGFVVPDAIPPIIEKVAIKPVDLSSTVNGLHSWAIMPFSYDEKQKVYTLPDTL
                     VVDGEFGIALSAFDRMNGLRRRFGIYQMKVLLDGELAFFSSYDSLDFDKTYKIDLERK
                     YLSKINKKEEFYELFLEPGNDLNLYLQNKGTINTQAVDSLGKKIYQRGYHQVEILVFD
                     ASHNFSLARFYLLFDQSPKLKQVSSEPFENNTKVKVEVLNKYEVSKVHLQTTRLDSIF
                     WGDTIISQQDSQDIYSFILSREPQMVRVRCEDRFGNFSDYKYMLINQDKLSKPPDGYT
                     PSSVRMDYAFDKGNFIFYLFFDKLVLEQPDVWLEAF"
BASE COUNT         2583 a         1985 c         2107 g         2730 t
ORIGIN      
        1 aagattaggt ctaataattg acaagatgtt agccagaaaa atagaggata ggtataagac
       61 tatagatgaa ctcgtctccg atattgaaga ttttgaagag gagaacaggg gctctcaaat
      121 ttatttggat tcacctgacg aacatcagac agacaaaccc atcgtccaaa cagaaacaga
      181 aaaagccctg ggaagattcg ctccttatct atttggattc ttgctcgcaa ctgcattttg
      241 gttgatcgtg tatcttctct taagatcata gataatcctt ctcatacgga gcgcatccaa
      301 atggatgcca caccccgcct cgatgtgatg caaaactata tcaaaactcg tatgctattc
      361 ctttgacctt caagtgagac tggtttcttc aggctttatg tcctctccct tagagggaga
      421 ggacataagt gagggtgaaa ctgttttctt ttgttccagt ctcgcagaca gaggatgagg
      481 gaaggtgcta tggctgtgag gtttaaactt ttccccctca ctctttctct ctccccttgg
      541 ggagagagaa actgtaggaa tcagccataa ggcattctga catgtcttga aaaaagtggg
      601 gatactcctg catctttttt cctgcatctt tttctcttga caaaacccga tcttggctta
      661 taattgagat atccaaactt gctgaggggg caggtcggtt tggtttttta attgcctcaa
      721 aataatctaa gaccaaggag gttctatgtt gaacaagctt tccttaattt ttatgttttt
      781 attttcaatg tttgtttttc taaccttgcc cgctgtggca caaaatccag actcaatccc
      841 gttcgccccg gcggtcaact atggggcggg agataagccc ctttctgttt tctgtgccga
      901 cctcgatggg gacgccgatc tagacttagc tgtggcaaat aatggtagcg acaatgtctc
      961 catcttaaag aacaatgggg atgggacctt cgccagtgcg gtcaactatg gggcaggaga
     1021 tggtgctttt tctgttttct gtgcggattt agatagggat aacgatttag acttagccgt
     1081 ggcaaacgta ggcagcgaca atgtctccat attgaacaac ggaaacggca tgtttgccag
     1141 cgcggttaac tatggggcag gagattatcc ttcttctgtt ttctgtgcgg atttggatgg
     1201 tgacaccgat ctggacttag ctgtatccaa tcattatagc cacaatgtct ctatcttaaa
     1261 gaacaatggg gatgggacct tcgccagtgc ggtcaactat ggggtaggat attatcctcg
     1321 gtttgttttc tgtgcggatt tagatgggga caatgattta gacttagctg tggcaaatga
     1381 tggtaatgac aatatctcta tcttaaagaa caatggggat gggaccttcg ccagtgcggt
     1441 caactatggg gcaggagatg gtgcctggtc tgttttctgt gcggatttag atggagacaa
     1501 cgatttagac ttagccgtgg caaatgcttg gagcgacaat atctccattt tgtttaacct
     1561 gacgcaatct attggctcga tttcgggaac tgtgtcggac tcacttggtg gactgctggg
     1621 tatcacggtt gacctatttg acaacacggg ctcgctggtc tggtttgccc acacggatcc
     1681 atctggtttt ttctccttcg acagccttaa cgctggtgac tatattgtct ccatagttac
     1741 ccctttgggc tacgtcgcag actttgagtc gagactaata acggttaccc cgaacgcaga
     1801 tagcgttgtt aacttcatgc tccatcacct tgaggttacc agctcacaaa gaagcatggg
     1861 ctactggaaa caccaggtta atgtttatct ctcgggtaaa ggcagcgcac aagaaagtct
     1921 ggctaacatg tccaaataca tgagtaggat caagactcat ttcaatgata acttggctaa
     1981 tccagttacc attttccaag tgccgcagcc tgctacaggg agggatagtc ttgaagccct
     2041 tcagagactc ctgacagtta acaaagatgg aacggcaaat gacagggcta aacagcaact
     2101 aatagcttta atgctcaata ttgtctcatc taaactccat cagacaacgg cagtcagcca
     2161 agatggtgct accgcatccc aggcagttac ttattgcaat gagcttatca cagacccaga
     2221 cccgtctaac gacgagattg caaaggatat tgctgacaat atcaacaatg gccttttaat
     2281 ctcagctggc ataattccaa ccactacacc cattatcgtg tacaagtaca aaggtggttc
     2341 cgagggtgat tcagaagggg atcttattga agcttcggat gtcccgaagt cgttcggatt
     2401 gctccagaat tatcccaacc ctttcaacct gtcaaccaaa atagagtttg gcttaaagaa
     2461 tccaggtttt gtcagcttga atatctatga cgttttagga agaaaggtga gaactttggt
     2521 ttctcaagac ctaccttcag gttataagtc tgtgctttgg gatgggaaag atgacaaagg
     2581 tcttgaggtg gcaagcggaa tttacttcta caggattcaa gctggagatt ttgcagaatc
     2641 gaaaaggatg ttgttactga aataactgct aactggcaga agaaaacgag cagccctaac
     2701 tctctcggag ttggggctgt ttttttctgt gttgtccagg agaaacgtct tcaaagaggc
     2761 aaattgaagt gttcatctag gggtcggatt catccgacct gacaagacag cgagttcgat
     2821 aaatcaaacc cctacgcaga gtttcaggtc aagctaaagc ttgacactcc agcagaacct
     2881 tcctgtcaaa actcttgact tagaacccgg caagagataa attaagacca aaagcaaaaa
     2941 tcttaaaaag gagtgtcaaa tgcacaagca ggataaaatt attctgctcg cagtagcatt
     3001 gtttattatt tttcctcaaa cagttttggc tgtcggaggt gtggctatag catttccttc
     3061 gccctcccct tgccctcaag gattggctta tgacggagca tatctttggc tggtggacag
     3121 gaaaacagat atgatttata agattaatcc caaagatgag tcgattattg actcgattcc
     3181 ctctcccggg tatgtgcctc ggggtttgac ctgggatggt aagaatctct ggtgtgtgga
     3241 tgcagaggag aatctgatat actccatcaa tcccaagacc agaatagttg agaagaccat
     3301 atcttctcca gcttctgacc ctgaagggtt ggcttgggat ggaaaatatc tttggattgc
     3361 tgattccaaa gaagctaaga ttgaccaact gagcacagat gatggaacca caattacttc
     3421 tattccctca ccctcgccct atccttgcgg tcttagtttc gatggaaaat atctctgggt
     3481 ttcggataga gcaagaaata tgatttatat gattactccg gacaagggag aggtaatcgt
     3541 ctgctttgac gctccgggtc cttataccta tggtttagct tgggacggaa aatatctctg
     3601 gaacgttgat taccagacag acaaaatcta taagctgaaa atagatgaca gcgaactctt
     3661 ttctcgtaaa gagcaaaagg aagaaacgct tgagtttact catcagataa gaaattatgg
     3721 cccagatacg gttaagactt tagatgttta tcttgctata ccggaaaacc taaataatca
     3781 ggagcttgtt gggccagttg agttcaatcc ccggcccact gatattttga ctgataagtg
     3841 gggacaaaag gtcgcccatt tccagttcaa agatttggga cctacccatt tcaatacagt
     3901 ttccatgaaa gccaaagtca agctctacca agtacgatat tttgtctttc cagagaaagt
     3961 tggtaagtta gaagatattc caaaaccaat aaaagagaaa tacttggtgg acgatgctaa
     4021 atattctatg aatcatccaa taattcaaaa tgcagtaaag aaagcagtgg gagatgaaaa
     4081 aaacccttac tggatagccc gcaagatatt tagttacatt attgataata tggaatacga
     4141 gagagtggga ggttggaaca ttgctcccgc ggtcctggct cgaggaaatg gttcctgttc
     4201 agaatatagc ttcgtctata tcgctatgtg ccgtgcggct gggttgcctg ctcgctatgt
     4261 tggctcagtt gcaatacgag gagatgatgc ttcctgggat gatgtttttc atcgctgggt
     4321 cgaagtttat cttcccaact acggctggat accggttgac ccttcaggcg gggattccaa
     4381 atggcccgct gaccaagcca atgcttttgg atatctgaac aacagatact tgattactac
     4441 aaccagcggt ggtgggtctg aatatttaga ttggagctac aattccaacg aaaagtggac
     4501 ttcggaagga aaatgcaaga tcgtggtgga gaatatcggt gagtggtcac cattaaatca
     4561 gttttgagtt tcaagttttg agtccctacc cctcaccaag tcagtttggg gttttgggtt
     4621 tcaagacaaa aaaatcttgc gtgcctgtca agtatgaacc atacgattcg caccgtaggg
     4681 atgggaattc atcccatccc gtgcggggct aaatgccccg cactacgaga agttttatga
     4741 aagacatatc agaatacccc cgtaggggaa taaatgacat ttctataagt tccctctccc
     4801 ttagaggaga gggttggagc ctgccctgag cgaaaccctg caccagattg gtgcagggcg
     4861 aagcgaaggg gtgaggggga gacaaagaaa gaacctcacc ctcaccttta tcctctccct
     4921 caagggagag gaggttattt tttcgaacag gcgagatacc gtaccctttg ggtgggaaag
     4981 ttaacttaat gaacaaacct aaaaatctac tcctacattg aatcacgatt tttaggagtt
     5041 taaatcaaaa tcatgaaact cttaggaata gtcggctctc ctcgggaaag aggaaattca
     5101 tttctcttga tggaaaaggt gatgcagtct gccaagaagg agaaaccaac tttactgact
     5161 gaaatcatcc agttgtcgag acaaaagata aaaccgtgta gggcttgtca aagctgtgca
     5221 gatgaccctt atatctgtat gcagcaagat gacctgaatg aaatctataa aaggatgaaa
     5281 aaggcagatg gaataatatt tgccactccc agttatggtc caattggagc ttgcccttcg
     5341 gttatgcagg cttttttgga aaggatagca aacctcagtc acctgccttc tcttaagaat
     5401 aagaagtttg cctttccgct taaggataaa ccctgcgggt taattgtagt ttcaattgaa
     5461 agccaagaga aagcccttgt ggttttgaac aacttggagc aatacgtttt ggcttatggg
     5521 atgaagccgg tgcatatcaa gaactcgtcc tcattaggag tgagtggtaa ggggaatgag
     5581 gcaggggatg tgctggatga caagggggca gtggaggatt caaaacttct cggaaaactt
     5641 ttggctaagg ctatttctcc acagctacgc ctggattaaa actattccgt tttttgtatt
     5701 tattgtagga attttgttcc gcacagggat aaattccctc tgctacgaac ataacctacc
     5761 gtaggtgtgg gaatttcctt cgaccttgct caggccttcg gctctgaggc tcaggctcga
     5821 agagatggtg aacttatcct tcgactttgc tcaggacagt gaagacgttc ggctgagcgg
     5881 ttcgaccgtc ccgagcggct caccgtcgaa ggactcagtc gaagccctcg tcgaactgtt
     5941 gaaccatatc ccacaccgtt cttgaaatcc agacttcgga caacaactta tagaaaatat
     6001 tccccaatat ttgagagaga cccaaactat tcggtcttat agaacttgtt tcgataatcc
     6061 tcgactttgg cttttttctt tatctgttca aaccaatcct gataagcctg cgttttcttt
     6121 tgtatcacca actgctgata tagtgaatct ttcgcttgtg tgaactcctg ttcgcttatg
     6181 ggaatatggg agacgaattc aatcaagtaa attccgtttt cggtcctcaa gggggggctt
     6241 attttatttt tttcattcaa ggaaaatgca gttccgacaa accgaggatt gtcatcaact
     6301 tgcgcgagct cggaactcct tgaaaagtaa tccaatctct gtattttctg attaaccttg
     6361 ctggttgcgg tttccaggtt cttcgttttt tctgcctctt gataagtttt ttgggcctca
     6421 cctaaagcta agtcgccggc cttattcaaa acaattctat ctttaatctt ttgtttcact
     6481 tcgtctaagg gatatatgcc ggctggtttt ctgtccttga cttccaccac gtaaaaacca
     6541 ttcttggttt caatcgggtc gcttgtttta cccaccttat tttcaaaggc aaaaagattg
     6601 gccccaagat tgtatcccat ttccgggatg atgctgtccc tgaagaagaa atcagttgag
     6661 gagaagttta agttgttttg cctggctgcc tctttcaagc ccaccttggt ggcttgctta
     6721 accaggtctt cagccataac ccttaaactg tcgatggtct gttcagtggg ggttatcttc
     6781 aaaagtatgt ggctagcatg aagctcctca tttttaccaa cccctctttt ttccaataac
     6841 ttaatcaagt gccaaccata ttcagttcta atgggttggg agatttcgcc caccttgagt
     6901 gaaaaagcgg cttgttcgaa tgattcaatt aacttacccc tggtgaacca gcccaaatcc
     6961 ccacccttct cggcagtaac tttatcatcc gaatagtact gcgccagcgt agcgaaatcc
     7021 tcaccctgct ccgcctccat tttaattctc aggaggtcgg atttggtttt ttgtatatca
     7081 tcttgagtag gcaccttggg aatcaccaca aagttcaagc ttgttctcga ctctactttg
     7141 aattcaccct gattctcttt gtagtattct tgaatctcct gttctgtaat tgggggaact
     7201 tttcccagaa actcgctatt tgatataaaa aggtaattaa ccttaacttt ctcgttgtcg
     7261 ttcacatatt cttgtttaac ctccgggtca gctactctga ccaggtcaac aatcatctcc
     7321 tgcaatttaa gggttatcag atttgcgcgg atatatccct cccaacgcca ccaagggaac
     7381 tgtggattaa ctaaggcttg catatacttc tggtaatcga attttccatc gatctgaaag
     7441 tcagggctct gttggaattc cgctggagga tacatcttca tatattcgaa aagctctttg
     7501 tcagatacgg ttatacctaa ctttctggtc tgttgctgaa cgagtatctc atctaccaaa
     7561 ttatcccagg tgttatccag aacctgtttg gtggtctgtt catctaattc ctcggactgg
     7621 tttcccagtt gtttcagctg attgtcataa aggttggcaa agtcggcagc gcttatctcc
     7681 tgaccattgg ccgatccgat tatacccttt ttatatttcg atttggttat atctccaccc
     7741 cacaccagaa cgatggttcc cacaaaggcg atcaccacaa tccacatcac gggcttgatg
     7801 atatcccgca tacctttcat caccggcata ttttgctcct taatttatcg gttaaatata
     7861 atttcaaatc gaaaattggc aagcttttta attgtctttt ttaacaaagg atttgacaaa
     7921 aaattttttt gaacgtagaa tagaatgtgc tcaaaaaaat tatcatctta ctatttctca
     7981 taacctcatg ctctccctct ctggcaggtg attatctctg gccacttaaa ggatatgatt
     8041 tattgtcctc ggttttcggg gattatcggg attttcattt ccatgccgga atcgatatca
     8101 ccaccggagg gaaaactgga gctaagatta ccgctccgac agacgggacc gtctttcgag
     8161 tttttacttc ttatcaaggt tatggtaagg cagtatatct gaaagcagag gatggtaaga
     8221 ttttcgtctt tgggcatctt tcggatttct cacccaagct atccgggcta gtggagagcg
     8281 aacaactgaa aaacagaaga tattatattg accttctcac tagtgggata aagataagaa
     8341 aaggagaagt gataggttat tccggtgaga gcgggtacgg aggaccacat ctgcattttg
     8401 aaatgagaga cgggggaaat cacccgatga atccactggt ttttggtttc gtggttccag
     8461 atgcaattcc cccaatcata gagaaagtag ccattaaacc tgtggattta agctccacgg
     8521 tgaatggtct tcattcgtgg gccattatgc cattttccta tgatgaaaaa cagaaggttt
     8581 ataccctgcc agataccttg gtggtggacg gcgaattcgg catagctctt tcagcttttg
     8641 accgaatgaa tgggttaaga cgcaggttcg gaatctatca gatgaaagta ttgctggatg
     8701 gagaactggc cttcttttca agttacgata gcttagattt tgataaaact tacaagatag
     8761 acctggaaag aaagtatctc tccaaaataa ataagaaaga agaattctat gagcttttcc
     8821 tggagccggg gaacgatttg aacttatatc ttcaaaataa aggaacaata aatacccaag
     8881 ccgtagatag cttaggtaag aaaatatatc agagaggata ccaccaagtt gagattttag
     8941 tttttgatgc cagccataat ttctccttgg ctcgctttta tcttcttttc gaccaaagcc
     9001 ctaagttaaa gcaggtttca tccgaaccat ttgagaataa tacaaaagtt aaagtggagg
     9061 tgctgaataa gtacgaagtg agtaaagtcc atctccaaac taccagattg gatagcatct
     9121 tctggggaga tacgataatc tcccaacagg attcacaaga catatattcc ttcatccttt
     9181 caagggagcc gcaaatggtt cgggtgagat gtgaggatag gtttggaaac ttctcggact
     9241 acaagtatat gctgataaat caggataagt tatcaaagcc accggacggt tacacaccat
     9301 cttcagtcag gatggattac gcttttgaca agggcaactt catattttat ctgttctttg
     9361 ataaattggt cctggagcag ccggatgttt ggttagaagc ttttg
//
LOCUS       DASWLN010000087         4074 bp    DNA     linear   ENV 20-FEB-2024
DEFINITION  MAG TPA_asm: Candidatus Zixiibacteriota bacterium isolate
            SMAG_U2949 k141_588337, whole genome shotgun sequence.
ACCESSION   DASWLN010000087 DASWLN010000000
VERSION     DASWLN010000087.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37960594
            Sequence Read Archive: SRR10376091
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Candidatus Zixiibacteriota bacterium (soil metagenome)
  ORGANISM  Candidatus Zixiibacteriota bacterium
            Bacteria.
REFERENCE   1  (bases 1 to 4074)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 4074)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (26-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/12/2024 08:28:26
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 1,440
            CDSs (total)                      :: 1,416
            Genes (coding)                    :: 1,412
            CDSs (with protein)               :: 1,412
            Genes (RNA)                       :: 24
            rRNAs                             :: 1 (16S)
            partial rRNAs                     :: 1 (16S)
            tRNAs                             :: 21
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 4
            CDSs (without protein)            :: 4
            Pseudo Genes (ambiguous residues) :: 0 of 4
            Pseudo Genes (frameshifted)       :: 0 of 4
            Pseudo Genes (incomplete)         :: 3 of 4
            Pseudo Genes (internal stop)      :: 1 of 4
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..4074
                     /organism="Candidatus Zixiibacteriota bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_588337"
                     /isolate="SMAG_U2949"
                     /isolation_source="soil"
                     /db_xref="taxon:2053527"
                     /environmental_sample
                     /country="USA: Oregon"
                     /lat_lon="42.99 N 123.63 W"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            complement(<1..2651)
                     /gene="rpoC"
                     /locus_tag="VGB16_02865"
     CDS             complement(<1..2651)
                     /gene="rpoC"
                     /locus_tag="VGB16_02865"
                     /EC_number="2.7.7.6"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_014559773.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0000345 - cytosolic DNA-directed RNA
                     polymerase complex [Evidence IEA];
                     GO_function: GO:0003899 - DNA-directed 5'-3' RNA
                     polymerase activity [Evidence IEA];
                     GO_process: GO:0006351 - DNA-templated transcription
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="DNA-directed RNA polymerase subunit beta'"
                     /protein_id="HEX9916654.1"
                     /translation="MANSPVKSAKRPLDFKAIKIKLASPEVIRSWSHGEVTKPETINY
                     RSFKPEKDGLFCERIFGPVKDWECSCGKYKRVRFRGIVCDRCGVEVTQSKVRRERMGH
                     IQLAVPVSHIWYVRSIPTRIGYLLDLSIRDLEKILYHESYIVIDPGNTSLKYKDIIPE
                     EEYARLEEEGKEFVAKMGGEAILELLKQVDVEELSISLRAQVKVETSAQRKKDTLKRL
                     RVIEAFRQSQNRPEWMIMNIIPVIPPDLRPLVPLEGGRFATSDLNDLYRRVINRNNRL
                     KKLIEIKAPEVILRNEKRMLQEAVDALFDNGRRSRSIRGDSRRLLKSLSDLLKGKQGR
                     FRQNLLGKRVDYSGRSVIVVGPELKIHQAGLPKSMALELFKPFILMRLEEKGYVQTVK
                     SAKKLVEKERPEVWDILEEIIQDHPILLNRAPTLHRLGLQAFYPVLVEGKAIRLHPLV
                     CAAFNADFDGDQMAVHVPLSFEAQLEAKVLMLSVNNLLLPSSGRPVATPSQDIVLGCY
                     YLTKEKPGDKGEGKVFVSTDEILAAYDARAVGLHAKIRVKMEGKLVETTVGRVLFNDI
                     IPQELGFFNDQATKGKLEDLISEAHRILGSYRTSVFLDELKRLGFEYATLAGITVGID
                     DMVIPKEKEELVKEAFKEVEKVQKLYERGVITNGERYNKVIDIWTHTTSQVAEVMFEG
                     MKEVDHGFNPVYMMADSGARGSKEQIRQLAGMRGLMAKPQKKITGQIGEIIESPIIAN
                     FREGLNVLEYFISTHGARKGLADTALKTADAGYLTRRLVDVAQDMIITEEDCGTILGV
                     NVGALKEGEEVIESLKDRVLGRVALEDMHDPITGDLLVKSREEINEQSSSAIDESGID
                     TAKIRSVLTCESKRGVCARCYGRNLATG"
     gene            complement(2665..>4074)
                     /locus_tag="VGB16_02870"
     CDS             complement(2665..>4074)
                     /locus_tag="VGB16_02870"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_013247879.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DNA-directed RNA polymerase subunit beta"
                     /protein_id="HEX9916655.1"
                     /translation="QVRDTKRGTEEITREIPNVSEEALVNLNERGVVRMGAEVEAGDI
                     LVGKVTPKGESELSPEERLLRAIFGEKAGDVRDASLKAPPGMKGIVIDTKVFSRKERS
                     EESKKQERNEIARVRRQFQKQIDSLKEIRNSKLGALLEGSTAKSIRKSTDGTIVFRAG
                     YKFKADSFKGIEVDELASDDGYTTDKKLNHKAEQFLANARKLIDSKKEQLELELEKVI
                     RGAELPPGVVQLVKVDVATKRKLSVGDKVAGRHGNKGVVARVVPEEDMPYMPDGAPID
                     IVLNPLGVPSRMNVGQILETHLGWAAKKLNLYVASPIFDGANLKEIKALLKEAGLPES
                     GKSILYDGMNGEPFDYPITVGYIYMMKLSHLVDDKIHARSIGPYSLVTQQPLGGKAQF
                     GGQRFGEMEVWAMEAYGAAYCLQEMLTVKSDDVAGRSRVYEAIVKGENPPEPGVPESF
                     NVLVKELQSLGLDVELVES"
BASE COUNT          993 a         1153 c          753 g         1175 t
ORIGIN      
        1 ccggtggcca aattcctacc atagcaccta gcgcaaactc ccctcttgga ttcacaggtg
       61 agaaccgatc tgatcttggc tgtatctatg ccactctcgt caatggcaga agacgattgt
      121 tcatttatct cctctctaga cttaaccaat aagtcaccgg ttatgggatc atgcatatcc
      181 tccagggcta ccctgcccaa gaccctatct ttgagagatt ctatgacttc ctctccttct
      241 ttcaatgcac ctacgtttac acccaaaatg gttccacagt cctcctcggt gataatcata
      301 tcttgagcca catccaccaa tcttcgggta agataaccag catctgcagt tttcagggcg
      361 gtatcagcca atccctttct ggctccatgc gtggaaatga agtattccag gacattcaaa
      421 ccttcacgga aattggcaat gataggagat tcaattattt cacctatttg gccagttatc
      481 tttttctggg gtttagccat taaacctctc atcccggcta attgtctgat ctgttctttg
      541 ctgcccctag ccccggaatc agccatcatg tacactgggt tgaaaccatg atccacttcc
      601 ttcataccct caaacatcac ctcagccacc tggctagtgg tatgagtcca gatatctatc
      661 accttgttat atctttcacc attagttatg actccgcgtt catacagctt ttgcactttc
      721 tccacctctt tgaacgcctc cttgaccagc tcttcctttt ctttaggtat aaccatatcg
      781 tcgatgccca cagttattcc agctaaagtg gcatattcaa atccgagccg cttcagctca
      841 tcaagaaaaa cagaggtacg ataacttccc aggattcggt gagcctcaga tattaaatcc
      901 tctaattttc ccttggttgc ttgatcgttg aagaacccca attcttgtgg aatgatatca
      961 ttaaagagga ccctcccgac tgttgtctcc actaatttac cctccatttt aactcttatc
     1021 ttagcatgca agccaactgc acgggcatca tatgcagcca gaatctcatc cgtcgaaaca
     1081 aagaccttac cctcaccctt gtccccaggc ttctccttgg ttagataata acatcccaag
     1141 acgatatctt gactgggggt agcgaccggt ctaccgctcg aaggaagtaa caaattgtta
     1201 accgaaagca tcaatacttt cgcctctaac tgtgcctcaa aggataaagg cacatgcact
     1261 gccatctggt caccgtcaaa gtccgcattg aaagctgcgc agaccaaagg atgtagtcga
     1321 atcgccttcc cttccactag caccggatag aatgcctgaa ggcccaggcg atgtaaagtc
     1381 ggtgcgcggt taagcagtat gggatggtct tgaattatct cttctaatat atcccagacc
     1441 tctggcctct ccttttcaac caactttttg gctgatttga cagtttggac ataacccttc
     1501 tcctccagcc gcattagtat gaaaggcttg aaaagttcca aagccatgct cttgggtaaa
     1561 ccagcctgat gaatctttaa ctctggtcca accacgatga ctgacctgcc agaatagtca
     1621 acccgctttc ccaaaagatt ctgtctgaat ctcccctgtt tacctttaag taagtcggaa
     1681 agggacttca ataatctcct ggagtcgcct ctaattgagc gagaacgtct accgttgtca
     1741 aatagggcat ccacggcttc ctgcaacatc ctcttctcat tgcgcaggat gacctccgga
     1801 gcttttatct ctatcaactt cttgagcctg ttgttgcggt tgatcacccg gcgataaagg
     1861 tcgttcaggt cggaggtggc gaatcttcca ccttccagcg gaactaaagg tctcaaatct
     1921 gggggtatca caggaataat gttcattatc atccactcag gtctattctg cgactgcctg
     1981 aaagcctcta taaccctcaa cctcttcagg gtgtctttct tcctttgagc tgaagtctcg
     2041 actttgacct gtgccctcaa agaaatggat agctcctcaa catctacctg ttttaagagc
     2101 tcaagtatag cttctcctcc catcttagcc acaaactcct tcccctcctc ctcaagccgc
     2161 gcatactcct cttcggggat gatgtctttg tatttaagtg aggtattacc aggatcgatt
     2221 acaatataag attcatgata tagaattttt tccaggtccc gtatagacaa atcaagaaga
     2281 taaccaattc gggtggggat agatctcaca taccaaatat gagaaaccgg tacagccaat
     2341 tggatgtgcc ccatcctctc tcttcgaact ttggattgag tgacttccac tccgcaacgg
     2401 tcacagacga tcccgcgaaa tctaacccgc ttatacttgc cacagctgca ttcccaatcc
     2461 ttgaccggac cgaatatcct ttcacaaaat agaccgtcct tctccggctt aaaagaacga
     2521 taattgatcg tttccggttt agtcacttcg ccgtgagacc aggatcgaat aacttccggc
     2581 gaagctaatt taatctttat ggccttaaaa tctaatggtc gctttgcgct cttaactggt
     2641 gagttagcca aagaaacctc cttcttagct ttccactaat tccacatcca ggcccaagct
     2701 ctgtaactcc ttaaccaaga cattaaatga ttccggaacg cctggttcag gtggattttc
     2761 tcctttaaca atagcttcat aaactcttga acgtccagcc acgtcgtctg acttcacagt
     2821 cagcatctct tgcaggcaat aagcagcccc gtaagcctcc atagcccaaa cttccatttc
     2881 accaaatctt tggccgccga attgagcctt tcctcccagg ggttgttgtg taaccaggga
     2941 gtaaggacca atggaacgag catggatttt atcatccacc agatgggaaa gtttcatcat
     3001 atagatgtat cccacagtta tagggtaatc gaaaggctct ccgttcatac catcgtaaag
     3061 aatgctcttg ccagactccg gcaaacccgc ttcctttaat agagctttta tctctttcag
     3121 attagccccg tcaaatatgg ggctggcaac atatagattc aacttcttgg ccgcccaacc
     3181 caggtgagtc tctaaaattt ggcccacatt catcctcgag ggaaccccca atggatttaa
     3241 cactatatcg atcggggcac catctggcat gtaaggcata tcctcttccg gcaccaccct
     3301 ggccactact cctttatttc catgcctgcc tgccaccttg tcaccaacag agagttttct
     3361 tttggtagcc acatccacct taactaattg taccactccc ggaggcagct cggcacccct
     3421 tatcactttt tccagctcca actccaactg ctccttcttc gaatctatca gcttccttgc
     3481 attagccaga aactgctccg ctttatggtt cagtttcttg tcagtggtat agccatcgtc
     3541 tgacgccagt tcatccactt cgataccttt gaaggagtct gccttgaatt tataaccggc
     3601 gcgaaaaact attgtcccat cagttgactt gcgaatgctc ttggctgtgc ttccctccaa
     3661 aagagctccc aatttactat ttcttatctc cttcaggctg tctatctgtt tctgaaattg
     3721 ccttctcacc cgggcaatct catttctctc ctgctttttc gactcttcgc tacgttcctt
     3781 gcgagagaaa actttggtat cgatgactat gcccttcatt cccggaggag ccttgagtga
     3841 agcatctctg acatctcctg ctttttcccc aaaaatggct ctcaggagtc gttcctccgg
     3901 agaaagctcg ctttctcctt tgggggtcac tttgcccacc aagatatcgc ccgcctccac
     3961 ctcagcaccc atccgcacta ctcctctttc atttaaattg accaacgcct cctctgagac
     4021 gtttggtatt tctcgggtaa tctcttcggt tccccgtttg gtatctctta cctg
//
LOCUS       DASWLN010000088         2264 bp    DNA     linear   ENV 20-FEB-2024
DEFINITION  MAG TPA_asm: Candidatus Zixiibacteriota bacterium isolate
            SMAG_U2949 k141_78904, whole genome shotgun sequence.
ACCESSION   DASWLN010000088 DASWLN010000000
VERSION     DASWLN010000088.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37960594
            Sequence Read Archive: SRR10376091
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Candidatus Zixiibacteriota bacterium (soil metagenome)
  ORGANISM  Candidatus Zixiibacteriota bacterium
            Bacteria.
REFERENCE   1  (bases 1 to 2264)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 2264)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (26-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/12/2024 08:28:26
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 1,440
            CDSs (total)                      :: 1,416
            Genes (coding)                    :: 1,412
            CDSs (with protein)               :: 1,412
            Genes (RNA)                       :: 24
            rRNAs                             :: 1 (16S)
            partial rRNAs                     :: 1 (16S)
            tRNAs                             :: 21
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 4
            CDSs (without protein)            :: 4
            Pseudo Genes (ambiguous residues) :: 0 of 4
            Pseudo Genes (frameshifted)       :: 0 of 4
            Pseudo Genes (incomplete)         :: 3 of 4
            Pseudo Genes (internal stop)      :: 1 of 4
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2264
                     /organism="Candidatus Zixiibacteriota bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_78904"
                     /isolate="SMAG_U2949"
                     /isolation_source="soil"
                     /db_xref="taxon:2053527"
                     /environmental_sample
                     /country="USA: Oregon"
                     /lat_lon="42.99 N 123.63 W"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            235..>2264
                     /locus_tag="VGB16_02875"
     CDS             235..>2264
                     /locus_tag="VGB16_02875"
                     /EC_number="6.1.1.9"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_020257983.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0000166 - nucleotide binding [Evidence
                     IEA];
                     GO_function: GO:0004812 - aminoacyl-tRNA ligase activity
                     [Evidence IEA];
                     GO_function: GO:0004832 - valine-tRNA ligase activity
                     [Evidence IEA];
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_process: GO:0006438 - valyl-tRNA aminoacylation
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="valine--tRNA ligase"
                     /protein_id="HEX9916656.1"
                     /translation="MNVKTKELPKHYSPKTVEEIWYGSWLQSGIFHAKLDSGKNPFSM
                     VIPPPNVTDILHLGHALNNTLQDILIRWKRMQGYEALWLPGIDHAGIATQVVIEKNLA
                     KEGKTRQSVGREKFIKMAWEWQEKNGQTILNQLKKIGCSCDWERSRFTLDEGFSQAVA
                     EVFVRLYNKGLIYRGNYIINWCPRCGTSISDEETEHQELEGKLWYIRYKLKSSDDFVT
                     VATTRPETMLGDTALAVNPKDKRYKKLIGSSAILPILDRELKIIADDRVDINFGTGVV
                     KVTPAHDPIDFEIATQHNLEKIVVINPDGTMNENAGKFKGMDRFECRKAILGELKKKK
                     LLEKEEPYKYSIGHCYRCGTIVEPYLSEQWFVKMKPLAKPAIEAVKSGELRFYPEHWS
                     KVYLHWMENIRDWCISRQLWWGHRIPVWYCQDCNQTIVSNKPPQECDKCKGKSLKQEE
                     DVLDTWFSSWLWPLSTFGWPKETQDFKHFYPTKSLFTASEIIFLWVARMVMAGFEFTG
                     KLPFNDVYIHGTVRDTNGVKMSKSLGNGIDPLDIIKEYGADALRASMMLVTPDGQDPC
                     ISVNTFDLGRNFANKLWNASKLVMLNCDGRKTDILASYKDLDLIDKWILSRLNRAIQE
                     VTNLMQNYRFNSSIKVLYDFVWHDFCDWYLELIKPRLSQTGTTQTNLPDGAVA"
BASE COUNT          646 a          466 c          574 g          578 t
ORIGIN      
        1 gacgaaggcg ggcttaaaag aaggcttcgg tcgcctttgg cgaacctcgc ttaaggcggg
       61 cgaagtttcg ctcgcctctc ggcgaagccg agggcctttt atatttgtga aacccccgta
      121 gggggaggct tcactctccg ctactctgct tcggacgatt caggtttgtc cttgacaaag
      181 agataggttt ttgttatcat caaacctgta tttctcaagg ggtaacctgt ccacatgaac
      241 gttaaaacaa aagagctacc aaaacattat agtcctaaga cagtagagga gatttggtac
      301 ggaagttggc tccaaagcgg catcttccat gccaaactgg attccgggaa gaaccccttt
      361 agcatggtta tacctcctcc caacgtgacc gatatcctgc acttagggca cgccctaaat
      421 aacaccctac aagacatctt aattcgctgg aaaaggatgc aaggatatga ggcgctttgg
      481 cttccaggga ttgaccatgc cgggattgca actcaggtag taattgaaaa aaacttagct
      541 aaagagggta aaaccaggca gtcggttggt cgagagaaat ttatcaagat ggcttgggag
      601 tggcaggaaa agaatgggca aaccatcttg aaccaactta agaagatcgg ttgttcttgc
      661 gattgggaga gaagtagatt cacgttggat gagggttttt cccaggcagt ggcagaggtc
      721 tttgtccggc tttacaacaa gggtttgatt tatcgcggaa actatatcat aaattggtgt
      781 cctcggtgcg gaacctcgat ttcagatgag gagactgagc accaggaatt agaagggaaa
      841 ctctggtata tcaggtataa gctgaaaagc agcgatgact ttgttacagt tgctactacc
      901 cgtccggaga cgatgttggg agatactgcc ttggcagtca atcccaagga caaacgttac
      961 aagaagctaa ttggaagttc agctatcttg cccatattgg atagagaatt gaaaataatt
     1021 gcagatgata gggtggacat caactttgga acaggcgtgg ttaaggtcac gccagctcat
     1081 gacccaattg actttgagat agctacccag cataatctgg aaaagattgt ggtgattaat
     1141 ccagatggga cgatgaacga gaatgccggc aagttcaaag gaatggatag atttgagtgc
     1201 agaaaggcta ttctggggga gctgaagaaa aagaagcttt tagaaaaaga ggaaccttac
     1261 aagtactcca ttggccactg ttatcgttgt ggcactatag ttgagccgta tctttcagaa
     1321 cagtggtttg tcaagatgaa gccattggca aaaccagcca ttgaggccgt caaaagcgga
     1381 gagcttaggt tctatccaga gcattggtcg aaagtttatc ttcactggat ggagaatata
     1441 cgcgattggt gcatttcccg ccaattatgg tggggtcatc gcattccggt ctggtattgt
     1501 caggactgca atcagactat cgtttcaaat aaacctcccc aagagtgcga taaatgtaaa
     1561 gggaaaagct taaagcagga agaggatgtt ttagatacct ggttttcctc ctggctttgg
     1621 cccctttcaa ccttcggttg gcccaaagag actcaagact tcaagcattt ttatcctacc
     1681 aaatcacttt tcactgcttc cgagatcatc ttcttatggg tggcaagaat ggttatggcg
     1741 ggctttgaat tcaccggcaa actgcccttc aacgatgtct atattcacgg cacagtcagg
     1801 gataccaacg gagttaagat gagtaaatcc ctgggcaatg gaatcgaccc tttggatatc
     1861 ataaaagagt atggagcgga cgcactcaga gcgagcatga tgttagttac tccggatggg
     1921 caggatcctt gcatttcagt aaacactttt gatttgggtc ggaattttgc caacaagctg
     1981 tggaacgcat ccaaattggt tatgctcaat tgtgatggaa ggaagaccga catcttggct
     2041 tcctacaagg acttagatct gatagacaag tggatactaa gtcgcttgaa tcgagccatc
     2101 caggaggtta ccaatctaat gcagaattac cgcttcaatt ccagcataaa ggtgctttac
     2161 gatttcgtgt ggcacgattt ctgcgattgg tacttagaat tgattaaacc tcggttgtcc
     2221 cagacaggaa caacacagac caatcttcca gacggggctg tagc
//
LOCUS       DASWLN010000089        23357 bp    DNA     linear   ENV 20-FEB-2024
DEFINITION  MAG TPA_asm: Candidatus Zixiibacteriota bacterium isolate
            SMAG_U2949 k141_462805, whole genome shotgun sequence.
ACCESSION   DASWLN010000089 DASWLN010000000
VERSION     DASWLN010000089.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37960594
            Sequence Read Archive: SRR10376091
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Candidatus Zixiibacteriota bacterium (soil metagenome)
  ORGANISM  Candidatus Zixiibacteriota bacterium
            Bacteria.
REFERENCE   1  (bases 1 to 23357)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 23357)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (26-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/12/2024 08:28:26
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 1,440
            CDSs (total)                      :: 1,416
            Genes (coding)                    :: 1,412
            CDSs (with protein)               :: 1,412
            Genes (RNA)                       :: 24
            rRNAs                             :: 1 (16S)
            partial rRNAs                     :: 1 (16S)
            tRNAs                             :: 21
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 4
            CDSs (without protein)            :: 4
            Pseudo Genes (ambiguous residues) :: 0 of 4
            Pseudo Genes (frameshifted)       :: 0 of 4
            Pseudo Genes (incomplete)         :: 3 of 4
            Pseudo Genes (internal stop)      :: 1 of 4
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..23357
                     /organism="Candidatus Zixiibacteriota bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_462805"
                     /isolate="SMAG_U2949"
                     /isolation_source="soil"
                     /db_xref="taxon:2053527"
                     /environmental_sample
                     /country="USA: Oregon"
                     /lat_lon="42.99 N 123.63 W"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            <1..586
                     /locus_tag="VGB16_02880"
     CDS             <1..586
                     /locus_tag="VGB16_02880"
                     /inference="COORDINATES: protein motif:HMM:NF012784.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003723 - RNA binding [Evidence IEA];
                     GO_function: GO:0004540 - RNA nuclease activity [Evidence
                     IEA]"
                     /codon_start=2
                     /transl_table=11
                     /product="RNB domain-containing ribonuclease"
                     /protein_id="HEX9916657.1"
                     /translation="LRSLKKACYQPEDIGHFGLAFRHYTHFTSPIRRYADLVVHRILK
                     SIKNDKYKLDRIVASKERLSQIGKQVSERERLSEEVERESIKLKQMEYLEDKLGEIYQ
                     GIITGFLSFGFFVQLNKLLIEGMVRLSSLEDDYYNYDENKGCLVGKHTNKRYTLGQSI
                     EVQVVRVDREQKQVDLVLVEKEGKPRKSKRQISR"
     gene            615..2078
                     /locus_tag="VGB16_02885"
     CDS             615..2078
                     /locus_tag="VGB16_02885"
                     /inference="COORDINATES: protein motif:HMM:NF025885.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_process: GO:0006355 - regulation of DNA-templated
                     transcription [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="sigma-54 dependent transcriptional regulator"
                     /protein_id="HEX9916658.1"
                     /translation="MEKKDKILVVSQAESFFGDIREILADVPTSVAWANSGAICLKMI
                     KEENFQLIVLDCNLSDLPGRSLVNKIIRRSPHSDILVALEEKEEKHKEVLNWGVDDIL
                     NSPLDKEEVHLKVTKLLRERNFLESCGLVGKSIELKKITEVVLQVAPTNITVMITGES
                     GTGKELIARAIHNNSPRKDKPLVIANCGALAEGVLESELFGHEKGAFTGAISRHKGLF
                     EQADGGSIFLDEIGEIRPHLQVKLLRVLEEKNFQRVGGNENIKVDVRVIAATNRKLEE
                     EVRKGNFREDLYFRLSVVKIDIPPLRERTKDIPILVLDFIEKLKFSSSNKLLGISDDA
                     LELLTRYHWPGNVRELRNFLESMSILVANRRIEKEQVVSYVEKQMEHERALPVATGKT
                     AETAEHEAIYQAILSLKSEIESLRQALQGNFEKSSVYEKDKEIPAQQSLEEMERELIV
                     RTLRSVRGNRKRAASLLGIGQRTLYRKLDKYGLKEIF"
     gene            2395..4041
                     /locus_tag="VGB16_02890"
     CDS             2395..4041
                     /locus_tag="VGB16_02890"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEX9916659.1"
                     /translation="MHKFFSYLLLMALVICCFISAYGQTISPEVFENQEDLKDALERG
                     IISYEEYLEFSELMSDKIKLDSDEKYKLLVIPGISWGDLVQKRDELYPQEERISGFVK
                     EPESKVNATATWQTYQKLKEEEDLSNRFSFRFIHKKFFDFNGEFSQEGERSLGTRRRS
                     IQFFLPKESFSLTVGNYDKRLGLGINIGYYSFLNYIRDDTLGAQDQFLYPLRARYNGF
                     LAEYQFNHIQTSVFYSANRWSDFMDWLWGGDLSWSFKKMDLGATFSWAEMKRVGKGGS
                     YEDRCISLHLETNFKTTNLLAEYAKMVGAGEGIGLVFTSDTKNYSVDWSFWNYASNFI
                     HLHNGGPSQSDYESTKIEEIDYTFKSRQAGERGINFVSRYKLTPNLRISLGYAQWSEN
                     RGLPQKMRAKIGASYSLWEKQSFILEKYWMDNNLEEEELDGEKTGLGFEISQFSNFKL
                     RIMADYGEKSLTGGKRKYGDIQFRISSSAISFSPLTLWLKCYDPNLNSSKDSTWRFYL
                     EQNMKFFKANSFILGLGAKYSRSTNSIEDKIVRTKLSINW"
     gene            4287..5168
                     /locus_tag="VGB16_02895"
     CDS             4287..5168
                     /locus_tag="VGB16_02895"
                     /inference="COORDINATES: protein motif:HMM:NF013125.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="lamin tail domain-containing protein"
                     /protein_id="HEX9916660.1"
                     /translation="MKKPNTLLFVLLLLSLTACAYAQGDVVLNEVLANEPKSWTTLEW
                     VELFNGSDEEINLAGWKLTSDEDTTVFDSIVITAGGYLVLARKLVSTMDSISFEGYWG
                     NNSGVWGDSPDENYPAVGVKISLTNTCDSVELIDTSGNTHKFIWTKDSGDGISWEKIN
                     PLLGDSANNWWVCIDSSGSTPGRKNSVNVSYSDAIKLELEPKVFSPDGDGFEDELKIK
                     YTLPIMSNLTLKVYDTKGRLVKILLEDEPRVSGEIIWDGKDDNNRFLKIGMYILYAYA
                     SGNTNSKAKVSFAVAKK"
     gene            5165..5989
                     /locus_tag="VGB16_02900"
     CDS             5165..5989
                     /locus_tag="VGB16_02900"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEX9916661.1"
                     /translation="MRSILKTIIIFFLFSSSSWAAFENWGESALVNSLGGEGVALGCE
                     AASLSYNPAGLGLLKKGLIESGYSQLFNLKDLETSDLYLVLPWRRLTYGMGIFIFGQK
                     EYYKETIFACGFAWQIKTPLFVGMNLKYMKVGFNSKYDNLSAIGLDGGVLLVPNRKIQ
                     FGLALRNFNQPKLISGSKDVPFSWATGTTINPFENFKLVFNLSQEETYSARVHLGQEI
                     RIFPKLVLRMGMASQPIRYSLGMGISLDRFCLDYSFLEHPVLGETHRINLSFSWKN"
     gene            6187..7074
                     /locus_tag="VGB16_02905"
     CDS             6187..7074
                     /locus_tag="VGB16_02905"
                     /inference="COORDINATES: protein motif:HMM:NF024816.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="tetratricopeptide repeat protein"
                     /protein_id="HEX9916662.1"
                     /translation="MKGVIHVLPSMFFLFIFILILGASHALAVEPKSETNPADAETNF
                     KIGVEYLIRGQYDQAIPCFDEALRINPSHAQTYNNRGLAYSYKGNYDRAILDFNKALE
                     INPENANTYINRGVAYCNKGEYDRAIQDYNKALEINPKDANAYYNRGNANDKKGEYDR
                     AISDYSKTLEISPKYAKAYGNRGATYCKKVQYDRAISDFNKALEIDPKHADAYYNRGT
                     AYYKKGNCDRAISDFNKALEINPEDAKTYINRGVAYEKLGKKREAIESYRSFIKYASP
                     NDLSLIEYTKQEIKKLENK"
     gene            7378..8523
                     /locus_tag="VGB16_02910"
     CDS             7378..8523
                     /locus_tag="VGB16_02910"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_004513342.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016793 - triphosphoric monoester
                     hydrolase activity [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="deoxyguanosinetriphosphate triphosphohydrolase"
                     /protein_id="HEX9916663.1"
                     /translation="MREELEGWEDKFLASYAAHSGKTKGRKFEEESHPYRTHYQRDRD
                     RIIHSTAFRRMEYKTQVFVNHEGDHYRTRLTHTIEVAQISRSIARALKLNEDLAEAIA
                     LVHDLGHTPFGHSGEEALNEIMVNYGGFEHNRQSLRVVDVLEEKYPQFPGINLTFEVR
                     EGIMKHETIYDQPIPVEFNPNTAPTLECQIVNLADEIAYNCHDIDDGLASNILKEEEL
                     NQVDLWAQLRQEVTKEFSNLNAFQLRNQVVRKMIDSQVSDLINETTRRLGKYHINSLE
                     DVRNFEEGLLSFSQEMKKKNSQLKMFLFDNMYRHYRMIRMADKAKRITRQLFDVYLND
                     VDQLPPTTRSRVREGNRMQAICDYIAGMTDRYALLEYKKLFDPMERV"
     gene            8601..10733
                     /locus_tag="VGB16_02915"
     CDS             8601..10733
                     /locus_tag="VGB16_02915"
                     /inference="COORDINATES: protein motif:HMM:NF012622.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004553 - hydrolase activity, hydrolyzing
                     O-glycosyl compounds [Evidence IEA];
                     GO_process: GO:0000272 - polysaccharide catabolic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="dockerin type I domain-containing protein"
                     /protein_id="HEX9916664.1"
                     /translation="MVVKYFKFAALTIVTLLPILFIPGINYARNFVFGSYMQPVTWYP
                     KYKYPEMSDSLKFNIIGDGYLYTGNALDSIKEAHLKVIIQNCEMGDYPCYYSSGHYTV
                     WEAEGELPEYHFTLTHNHGIGSAVYDNAASNLYAWKVDKNLPGSLPSIIQEGPGYRQE
                     MPVNDNSSDRNYTVEYRMRLDEVVTDSTQWDDPVAVLIVRRDSATDTLGAIDTVRVRD
                     FSEGISEPDTLYHSFILKYEPVAVYLLNLEIYWMAAHNLLIDKVTVYDDRGFELFNNP
                     SAAESIKAYVRQSWVDMTVYRWYLKDEATFSVDCFMPYRFVDSLLAEVNQERRAVTCV
                     FLRDDRYPSDPNMIAEFVKRTNPRQVNIENYVLNGHNDCGNTYAPLYYTTHSGEGYWP
                     PDSMGLQISLDGFTHTLEELNQSIDINSIDLWVSVQAFRWRWCCGWVRRTPTPEELRC
                     MSNLALIYGAKGILYWVYFSTDTVSGGGPCPDSTIIQWHGLVNWYGYEKTENWYEIKN
                     NIGPFIDKLGDTFCSLKWQGACSDDIVRYLVLRNGQPSYLDSIRSPGHEPHWVQVGFF
                     ENQTADTSYFMLVNRECLVTEGDSFDVFVNKSGGYYRIRDMYSDSIVGIVKGKGDYFK
                     AYLGPGEGKLLRLEKVVSGDVNGGGGRSITDVVYLITYLFKGGPPPKCPPQPYLSCAD
                     VNCDGLVNVTDVIYLINYLFKFGPPPGC"
     gene            10814..11602
                     /locus_tag="VGB16_02920"
     CDS             10814..11602
                     /locus_tag="VGB16_02920"
                     /inference="COORDINATES: protein motif:HMM:NF018449.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="SBBP repeat-containing protein"
                     /protein_id="HEX9916665.1"
                     /translation="MLRRFHLKAWFCLFFLILWALPVVAQDINMNKNNWRHYTPPENI
                     YNETVNGIATDDSGNTYVTGWSHNQETGNDYVTIKYNQHGDEVWVRKYNGSASLGDRA
                     NALAIDKLGNIYVTGWSRDNGTWDDYVTIKYYSNGDTAWTRRYNGPGNWHDGAWDIAT
                     DRSGNVYVTGWSWGEETKFDYATIKYDKNGKELWVRRYNGPGNSEDRALNIAIDKNGN
                     ACVSGISLVPGKYWDYVTIKYKPNGDCLWVKKYTRQGNPPVGIL"
     gene            12085..14805
                     /locus_tag="VGB16_02925"
     CDS             12085..14805
                     /locus_tag="VGB16_02925"
                     /inference="COORDINATES: protein motif:HMM:NF012622.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="choice-of-anchor Q domain-containing protein"
                     /protein_id="HEX9916666.1"
                     /translation="MSDAQVANGYVWFVSKNSPADHRGYILFGLWNEKPDVQNNTWHI
                     DYSREMHNPWDSLIYYARFNLKVPGGGYSPLDTVAKIEAHLMNLTQDRLVADSLLLYQ
                     DFDPDQYKIFELSFWKHPENANDSLDYKIYWYKKTDLYIDYVEVYDCYYDTLVNHKPM
                     YQNRIKSEIAPFENQYHGTSLFRWYLKDEPEYKYYKANREISDLLDSLGYAPGIQAVG
                     HEYIPKRFADEIQPHEICWDDYIIATWVVPHLLQYQLNQLTFRLGQVAAAAREHNVEW
                     WYVAQTYRESDSLARYPHNSELRATVYLSLAYGAKGMGYFRYSSAPWWTPETPIGQSG
                     LMWWNNLISDWVPVETYSNPNPYSNRPETLLTEVVRINFWLDSLGTTLRSLDWIDACV
                     DSSYNNNDLLGCGAGYIDSIRSHNSADEPHWVQVGFFENQTSDTSYFMLVNRECLVTE
                     GANYDVFVNKSGGRYRIRDMYADTIVGIVNGTGDHFTVYLGPGEGKLFRLEPIPKIKN
                     VPADYATIQSAINATWDGDTVLVAPGTYYENINFQRKAITVASDFIFDHNPATIQATV
                     INGSAHLSSVVSFGSGESSTSVLKGLTITGGTGTSWSGQGRKVWLGGGIYCTYSSPSI
                     TNNLIIDNHIPHYSGLGYQWGGGIGCQGGSSPIISNNLILSNSCANDSLGGGIYFAGG
                     RPVIVNNTLSGNTGGGITFSTTDYADSFVISNNIIANSTSGWGIRSEVVGPNTNGISY
                     NDVWNNAFGSFMGCPSGVGDTTWGTNANGTPCDSFHNIIRDPMFVNPDADYHLQRGSV
                     CVDAGDNNAIYMPNLDFDGKPRIAGKHVDMGAYENAGFICGDANGDGQITVSDVVYLI
                     NYLFKGGPPPECPPQPYLSCGDANCDGKVTVSDVIYLINYLFKGGPPPGC"
     gene            15034..15585
                     /locus_tag="VGB16_02930"
     CDS             15034..15585
                     /locus_tag="VGB16_02930"
                     /EC_number="4.1.1.19"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_013568332.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008792 - arginine decarboxylase activity
                     [Evidence IEA];
                     GO_process: GO:0006527 - arginine catabolic process
                     [Evidence IEA];
                     GO_process: GO:0006596 - polyamine biosynthetic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="arginine decarboxylase, pyruvoyl-dependent"
                     /protein_id="HEX9916667.1"
                     /translation="MIVIPKRIFLTKGVGKHKDKLNSFELALRNAGIARFNLVGVSSI
                     FPPSCKLISRKMGLEFLEDGQILYSVMSRNSTNEPNRLISASVGLAIPNDRNQYGYLS
                     EHEAYGWTERKAGDYAEDLAATMLATTLGLDFDVDKSYDQRKQIWKISGKIVRTTNMT
                     QSVEGDKNGLWTTVVAAAIFVTV"
     gene            15602..16630
                     /locus_tag="VGB16_02935"
     CDS             15602..16630
                     /locus_tag="VGB16_02935"
                     /EC_number="2.5.1.46"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_014405201.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_process: GO:0008612 - peptidyl-lysine modification to
                     peptidyl-hypusine [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="deoxyhypusine synthase"
                     /protein_id="HEX9916668.1"
                     /translation="MKKYLHSPTRPFEVRSDSKADDILERMGRISFQGRNLSIALSIW
                     KEMLKGNNVIFLGLAGAMVPAGMRKIISFLIKQRYIDCLVSTGANLYHDLHESLGKYH
                     WIGSCMVDDCDLQKKKIDRIYDTFASDREFRQDDNFIIDFVKTLDLKRTYTTREFLYL
                     LGRKLSVAGETEGILSSAYKARVPIFCPALGDSSIGIAMVWYLVQSGISPTFDIVRDA
                     AEISHITQKAKSTAVIYVGGGTPKNYIQQSEVIAAELRKGREIGHSYAIQITTDSPQW
                     GGLSGCTFAEAQSWGKIGLRAKKVDLHADATIVLPILVTALHQSLGKSFKRKHTPSFD
                     FKGKFDFG"
     gene            16868..17785
                     /gene="speB"
                     /locus_tag="VGB16_02940"
     CDS             16868..17785
                     /gene="speB"
                     /locus_tag="VGB16_02940"
                     /EC_number="3.5.3.11"
                     /inference="COORDINATES: protein motif:HMM:TIGR01230.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008783 - agmatinase activity [Evidence
                     IEA];
                     GO_process: GO:0008295 - spermidine biosynthetic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="agmatinase"
                     /protein_id="HEX9916669.1"
                     /translation="MPLQTQDKRKLDKDSFSKRNFLAISEEFSNLENSEFVILPVPYE
                     QTTTYKAGTREGPLAIINASAQVELYDEELGLESYRKGICTLDNLNVTSLGPKRMNEI
                     IYEASKELIHLKKKVVMLGGEHSISWGLVKAYRERYPDLSVLQLDAHADLRDEYQENK
                     FNHACIMRRIRELVPSVQVGIRNLSQEEAEYIKTQKKLPIFYAQKMISSDEWMDQAIV
                     LLSDEVYVTIDLDFLDPSIMPAVGTPEPGGILWYPTLNFLKKLARRKKIVGFDMVELA
                     PLPGMVAPDFLAAKLVYKLIGYLVEQKKI"
     gene            18024..21398
                     /gene="mfd"
                     /locus_tag="VGB16_02945"
     CDS             18024..21398
                     /gene="mfd"
                     /locus_tag="VGB16_02945"
                     /inference="COORDINATES: protein motif:HMM:TIGR00580.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003690 - double-stranded DNA binding
                     [Evidence IEA];
                     GO_function: GO:0005515 - protein binding [Evidence IEA];
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_function: GO:0016887 - ATP hydrolysis activity
                     [Evidence IEA];
                     GO_process: GO:0006281 - DNA repair [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="transcription-repair coupling factor"
                     /protein_id="HEX9916670.1"
                     /translation="MSLSSILSQASSSVSFKQLEREIASSKKWISLCEVAGSGKALLA
                     SYLYDISPTSILVITDKADEASTLVEDLENFVDKSKICYFPAWEVLPYEWRVPRGEII
                     GTRLETLYQLLSNQKVLVVSSLRGLMERTLPVAILKENIWNLKVGDKLQLDTLVGKLN
                     ELGFIRAVQVEEVGSYSSRGGILDVFPYSSSSPLRIELFEDKVESIREFSILTQRSFK
                     KVDSVLILPKREVLISDEKLEEYLSGIDEHKAERIREKIRFYDEVPGLEWLVSLLDIS
                     QSTLLDYLNPQDIVLSEDMSSLKSEYDNILQETEGLCQQAEKKGEIVPSPLKLWNSWE
                     EIKNNIVQHQLLEIKALRSKEGESLEFGMTEQEFFGGKMDFLKQRIEEYRKGSKKVYI
                     ICENATQKERLAELLDELSFEVHFEIAILHKGFSFNLADLVVLTEHQIFSRYFRRRRK
                     KRFKEGLALSSYTSLSIGDFVVHVDFGIGRYMGPETLLVDNKKRECLEIRYQDEDKLY
                     VPIEEFNRVQKFVGKEGGPALSKLGGTAWERLKSRTKKAICDMTKELISLYAERKAKP
                     GFAFSPDTRWQKELESSFIYEETPDQWETIKHVKKDMESPLPMDRLVCGDVGYGKTEV
                     AVRAAFKCVTNGKQVAVLVPTTILAQQHLATFIERLSSFPIRVELLSRFKTKPEQKKI
                     IEDLKQGKVDIVIGTHKLLQKDVGFKDLGLFIVDEEQRFGVAHKEKLKKLRTQVDVLT
                     LTATPIPRTMQLSLMGIRDMSLINTPPKYRLPIQTEIGKFEDELIADAILKEIDRGGQ
                     IYFVHNRVESIDSIYRYLKRILPQVRIAIAHGQMEEGVLEEVMLSFLDRKYDLLLATS
                     IIESGLDIPNVNTIIVNRADKFGLAQLYQLRGRVGRSNQKAYAYLLVPPFASLTESAR
                     KRIKALEQFTELGSGFHLAMRDLEIRGAGNILGAQQHGFIQEIGFDLYCKLLDEAVRE
                     LKGEKIEKLPEVKLDFDMDLYIPPDYISDSEQRVEIYRKLASAQSPQKIEEMALEMED
                     RFGLLPREVENLLEVVRIKLTAAKKGIVHLGLRGDILRIGFSKDRKIDKKQIEGFAKN
                     IEYPLEFSVSGDFTIKLDLKGREHKLEVVKNLLPHF"
     gene            21457..23172
                     /locus_tag="VGB16_02950"
     CDS             21457..23172
                     /locus_tag="VGB16_02950"
                     /EC_number="5.2.1.8"
                     /inference="COORDINATES: protein motif:HMM:NF024545.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="peptidylprolyl isomerase"
                     /protein_id="HEX9916671.1"
                     /translation="MKRIALLGLLLVLMVFGLVGCAKRENKVVAKMENREITLGELEN
                     RFFTQMKMAAASGMDIPKQEDILEDMIKREVLIISAYKRDLDKDQKIDTLLKEREKSL
                     LMDELFKVEILSKSKPTDSEVKSFYNKLKEKIRARHILVKTKTEAGSIYNQLKKGANF
                     EQLAKEKSIDKQSGQQGGDLGFFSWGNMVEPFQKTAFALKKGEISKPVKTQFGWHIIK
                     LEEREKAEPPDFQSNKEAYAQNLERMKQTELINQYLEGLKKRENFKVDSLTFNILLTK
                     ASQLDSTTGERRVVGGISVDIFTPQERQALLVQYRGGKMSLEDFALKFNLLPANSRMN
                     GFGDREGLKELAYQMLLPDLLVEAARLRGIDKSKSYRKKFLDIKEAQMASKLVNEFIL
                     DTVKVTPEEVEKYYQEHKNLYFVPPQVHIQEILLGSKQEADEVLKKVKSGADFSRLAQ
                     EKTLRTYSKDKAGDLGYIQEAQFPELFKAGLKLRKGEVGGPIYSKESMLGEEYSIIKL
                     LDKKEGKQTSFAEVREYVGETALREKQNKVLEDWIEKAKASMKLAVHKELLGVAKSDS
                     VKTGS"
     gene            23174..>23357
                     /locus_tag="VGB16_02955"
     CDS             23174..>23357
                     /locus_tag="VGB16_02955"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEX9916672.1"
                     /translation="MKRGFWVLALLMISCLAGCGGLGDKLEDKTVLAKVGDRILTAED
                     METEIPEEYKSSVTLEQ"
BASE COUNT         7071 a         4437 c         5696 g         6153 t
ORIGIN      
        1 tttgagatct ctgaaaaaag cctgctacca gccggaagat atcggacatt ttggattggc
       61 tttcaggcat tacactcatt tcacttcccc tatcaggcgc tacgctgacc tggtggtgca
      121 tcgcatactt aaaagcataa aaaatgacaa atacaaactt gatagaatcg tggcatcaaa
      181 ggagagactg tcccagatag gcaagcaggt ttcagagcgg gaaagactct cagaagaggt
      241 ggaaagagaa tccataaaac taaagcagat ggaatatctg gaagataaac tgggcgaaat
      301 ataccagggg ataataaccg ggttcctctc ctttggattc ttcgtgcagt taaacaagct
      361 tttaattgag ggaatggtca ggttaagtag tttagaggat gattattaca attatgatga
      421 aaataaaggg tgcctagttg gaaaacatac caacaagaga tataccttag gtcaatcaat
      481 tgaagtacag gtagtaaggg tcgacaggga acaaaaacag gtagatttgg tgctggtgga
      541 aaaggaaggt aagcccagaa aatcgaaaag gcaaatcagt cgataaagca ttacgacccg
      601 taggggcgtt ttgaatggaa aagaaagata aaatattagt tgtttcgcag gcagagagct
      661 tctttggaga tatacgggaa atattagctg acgtccccac cagtgtagcc tgggcgaata
      721 gtggagcaat ctgcctgaag atgataaaag aagaaaactt tcagctgatt gtcttggact
      781 gcaacttatc cgacctgcct ggcagaagtt tggtcaataa aatcatccgg cgcagtccgc
      841 acagcgacat cttagtagct ttagaggaaa aagaagaaaa acacaaagag gtcttaaact
      901 ggggagtaga tgatatctta aactcaccct tagacaaaga ggaagttcat ttaaaagtca
      961 ccaagctcct aagagaaaga aatttcttgg aatcctgcgg attggttgga aaatcaattg
     1021 aactcaagaa aattactgag gtggtcttgc aggtggcacc aaccaacatc accgtcatga
     1081 taactggaga gagtggaaca ggaaaggaac taatcgcccg cgccattcac aacaacagtc
     1141 ccaggaaaga taagcccttg gtcatagcta attgcggagc tttggctgag ggagtgctgg
     1201 agagtgagct ttttgggcat gagaaaggcg ctttcacagg agcgataagc cgacacaagg
     1261 ggttatttga acaggcagat gggggcagca tattccttga cgaaatagga gaaattaggc
     1321 cccatcttca ggtgaaactt ttgcgggttt tggaagagaa gaatttccag cgagtcggag
     1381 gaaatgaaaa cataaaagta gatgtaaggg tgattgctgc caccaatcgt aaattagagg
     1441 aggaagtcag gaaaggaaat ttcagagagg acctgtattt cagattgtcc gtggtcaaaa
     1501 tagatattcc tcctttaagg gagaggacta aggatattcc cattttggtt ttagacttta
     1561 tagagaagct caagttttcc agctcaaata aacttttagg gataagcgat gacgctctag
     1621 agcttttgac ccgttatcac tggccaggaa acgtcaggga actaagaaat ttcctggaga
     1681 gtatgtcaat cctcgtggct aatcggagaa ttgaaaaaga gcaggtcgta tcttatgttg
     1741 aaaaacagat ggagcacgaa agagcacttc ccgtagcaac cggaaagact gcggaaacag
     1801 cagagcacga ggcgatttat caggcaatct taagtttgaa atctgaaata gaaagtctaa
     1861 ggcaagctct acagggtaat tttgaaaaat cttcagtcta cgagaaggat aaggaaattc
     1921 cagctcaaca gagcttagaa gaaatggaaa gggagctaat cgtaagaaca ttaaggtctg
     1981 tcaggggaaa ccgcaaaagg gcagccagcc ttttggggat tgggcaaaga acactctacc
     2041 gcaaactcga caagtacgga ttgaaagaaa tcttttaaat ctcattttta aatattgtcg
     2101 aataggggtt gtctaagaag tgtcattgcg aggagtcctg agctatgtcg aaggacgacg
     2161 aagcaatctc ccacttgtca ttctgaacag agtgaagaat ctcctcaaag tgagatcctt
     2221 cgcttacgct caggatgacg cttgcgcttc agattgtcac gcccccgatc tcaagatgca
     2281 gaattcgcat aaatcggggt cgctcgccct gaaccagaac ggtttagggc tcgcaatgat
     2341 aaaaagataa aagagaaggt ttttggacag cctcagtatt cattcgtatt ccacatgcat
     2401 aaattcttca gttatttact actgatggcc ctggtaattt gctgttttat ttcagcttac
     2461 ggtcagacca tctccccgga ggtctttgaa aaccaggagg acctgaaaga tgctttggaa
     2521 agagggatta taagttatga ggaatacttg gagttttcgg aattgatgtc ggataagata
     2581 aaattagatt cagatgaaaa gtataaactt ctggttatcc cgggaataag ttggggagat
     2641 ctggtgcaaa agagagatga gctatatccg caagaagaga gaatatcagg ttttgtaaag
     2701 gagcctgaga gcaaagtcaa tgcgacagca acctggcaga cctatcagaa actgaaagag
     2761 gaagaggact tgtccaatcg gttttccttc aggtttatcc acaaaaagtt tttcgatttt
     2821 aatggagaat tctcccagga aggagagcga agtttgggaa caagaagaag gtcaatacaa
     2881 ttttttcttc ctaaagaatc atttagttta acagttggaa attacgataa aaggctcggt
     2941 cttgggataa atatcggtta ttattcattt ctcaattaca ttcgggatga tacgctcggt
     3001 gcccaagacc aatttcttta tcctctaaga gcaagatata atggctttct tgcagaatac
     3061 caattcaacc acatccagac ctctgtgttc tattcagcca atcgctggtc agattttatg
     3121 gattggcttt ggggtggaga tttgtcctgg tcttttaaaa aaatggattt gggcgcgact
     3181 ttttcttggg ctgagatgaa aagagttgga aagggtggat cctacgaaga tagatgcatt
     3241 agcttgcacc tggaaaccaa cttcaaaacc acaaatcttt tggctgaata tgccaaaatg
     3301 gtaggagccg gagagggaat tggtctggtt ttcacaagtg ataccaaaaa ctattcagtg
     3361 gattggtcat tttggaatta tgcttcaaat ttcatccacc tgcataatgg aggtccatcc
     3421 caatctgact atgagagcac caagatagaa gagatagatt atacttttaa atcgcgccaa
     3481 gccggggaaa ggggaattaa ttttgtatcc cgatataaac tcacaccaaa cttgagaatc
     3541 agtctgggat acgcccaatg gagcgaaaat agaggcttgc ctcaaaagat gagggcaaaa
     3601 attggagcaa gttattccct ttgggaaaaa cagtctttca ttttagagaa atattggatg
     3661 gataataacc tggaagagga agagttggat ggtgagaaga ccggtcttgg atttgagatt
     3721 tcccaatttt ctaatttcaa gctaaggata atggctgatt atggcgaaaa atcgcttaca
     3781 ggtggcaaaa gaaaatatgg ggacattcaa tttagaatca gctcttccgc tatatctttt
     3841 tcacccctga ccctctggct gaaatgttac gacccaaatc tgaatagctc caaagacagt
     3901 acctggcgat tttaccttga acaaaatatg aaattcttta aagcaaacag cttcatctta
     3961 ggtcttggag caaaatatag tcgctctaca aatagcatag aagataaaat tgtcaggaca
     4021 aaactttcca taaactggtg aaacctcccc tcaccttaat cctctcccca aaggggagag
     4081 gaaatcgaat cccctctccc cagtggggag agggaaatga aaacttaaaa attcgttacg
     4141 aattttagag ttttcattaa gggtgcctgc ccggccaggg agggggcaag tgcgacttta
     4201 agtcaagttg aaaagctgat gtgcataatc acggggcttc ccgaatataa aaggccctcg
     4261 gcagaggcga gtaaatgaat tcacaaatga aaaaacccaa cactcttctt tttgttttgt
     4321 tgttactatc tttaacagct tgtgcttatg cgcagggaga tgttgtccta aacgaagtct
     4381 tggctaatga gccaaaaagc tggacaactc tggaatgggt cgagctattt aatggttcgg
     4441 atgaagaaat taacttagca ggatggaaac ttaccagtga tgaggacaca actgtttttg
     4501 attccattgt cataacagct ggaggatatt tggttttagc ccgaaaacta gtatccacta
     4561 tggattcaat cagctttgaa ggttattggg gaaataatag tggggtttgg ggggactcgc
     4621 ctgatgagaa ttatccagca gttggtgtga agatctcctt aaccaatact tgcgatagtg
     4681 tagagttaat tgatacttcg ggaaatacac ataagttcat ctggacaaaa gatagtggag
     4741 acggaatctc ctgggaaaaa attaaccctt tattgggtga ttctgcgaat aactggtggg
     4801 tatgtattga ttcttccggc tcaaccccgg gcaggaaaaa tagcgtgaac gtaagttatt
     4861 ctgatgcgat aaaacttgaa cttgagccga aagtcttctc cccggatggg gatggatttg
     4921 aggacgagtt gaaaataaaa tatacccttc ccataatgtc gaatctaact ttaaaggttt
     4981 atgatactaa aggaagatta gtcaaaattc ttttggaaga tgagccccga gttagtggag
     5041 agataatctg ggatgggaaa gatgataata accggtttct gaaaatcggg atgtatatac
     5101 tctatgctta tgccagtggc aacaccaaca gcaaagccaa ggtcagcttc gcggtggcta
     5161 aaaaatgaga tcaatactta aaacaattat catattcttt ttattttctt cttcatcctg
     5221 ggctgctttt gaaaattggg gcgagagcgc tctggttaat agcctaggcg gagaaggagt
     5281 ggcattgggt tgtgaagcgg ccagcttatc atacaatcca gctggtttgg gacttctgaa
     5341 aaaaggattg atagagtccg gctattccca gctttttaat ctcaaagatt tagaaacaag
     5401 cgacctctat ttagttttgc cctggagaag attgacttat ggaatgggaa tctttatctt
     5461 tggtcagaaa gaatattaca aggagactat tttcgcctgt ggctttgcct ggcaaattaa
     5521 aactcctctt ttcgttggaa tgaatttgaa atatatgaaa gtgggattca attcaaaata
     5581 cgacaatctg tccgctatcg gattagatgg tggagttctc ttggtaccca atcgaaaaat
     5641 tcaattcggt ttggctttaa gaaatttcaa tcaacctaaa ttaataagcg gctcaaaaga
     5701 tgtacctttt tcctgggcaa ctggaacaac cattaaccct tttgagaatt tcaagttagt
     5761 ttttaatctt tcccaagaag aaacttattc tgccagagtg catttagggc aggagataag
     5821 aatttttcca aaattggttt tgcgaatggg gatggcaagc cagcccataa gatatagtct
     5881 gggaatggga ataagtttgg atagattctg tttggattat tcttttttgg agcatccggt
     5941 cttgggagaa acgcaccgaa tcaatttgtc gtttagttgg aaaaattagt tgatgaggat
     6001 tttgaaatct gttaaaaaaa tggtaagccc acgttgccct caacgtgggt cagcagtccc
     6061 accttgtggg caagcctgcc cggccagggg tggggatact ggttagatga ggattggaat
     6121 gtagaaaaaa gttgcgaaac tcgtcccgta aggggcctgg cattcaggtt cagaaaagga
     6181 gcaaaaatga aaggggtaat acatgtacta ccaagtatgt tttttctttt cattttcatc
     6241 cttattcttg gagcttctca tgctctggca gttgaaccta aatcagagac aaacccagca
     6301 gatgccgaga caaacttcaa gataggagtt gaatatctca ttagaggtca atatgatcaa
     6361 gctattcctt gcttcgatga ggccttgagg ataaacccaa gtcatgccca gacatataac
     6421 aaccgaggat tagcctattc ctataaggga aattatgacc gagctatctt agatttcaac
     6481 aaagctctgg aaataaaccc agaaaatgct aatacctaca tcaatcgagg agttgcctac
     6541 tgcaataaag gtgaatatga tagagccatc caagattaca acaaagccct agagataaac
     6601 ccaaaagatg ctaatgcata ttacaatcga ggaaatgcca atgataagaa aggtgaatat
     6661 gatcgggcta tatcagatta cagcaagact ctagagataa gcccgaaata tgctaaggca
     6721 tatggcaacc gaggagctac ctattgtaaa aaagttcaat atgatcgagc tatatcagat
     6781 ttcaacaaag ccctggaaat agacccaaaa catgctgatg catactacaa tcggggaact
     6841 gcctattaca agaagggaaa ttgtgatcga gcgatctcag atttcaacaa agccctggaa
     6901 ataaacccag aagatgccaa gacatacatc aatcgaggag tcgcctacga aaagctgggg
     6961 aaaaagagag aagcaattga gtcttacaga tcttttatca aatatgcatc tccgaacgac
     7021 ctttccctca ttgaatatac aaaacaggaa ataaagaaat tggaaaacaa atgactcaag
     7081 ctaactatta ggacaaagaa agaaaaaatt tcgaaagaaa aaaattgcag aactcatcaa
     7141 gaaacgaccc aacaggaaaa ttggcgggca taaagtgcat ccagccttat gccgatttaa
     7201 tctatatagt gttttatcaa ctaattaatg gtctgacaat attgtcaccc tgaacgaaag
     7261 tgaagggtct cgattttctt ctaaaagcga gattcttcgg ctacgcctca gaatgacaaa
     7321 gttgatcagg caatagagga taattgtttt ggaggtttta tggatatctg taaaaatatg
     7381 agggaggaat tagaagggtg ggaggataag ttcttagctt cctatgctgc tcatagcggg
     7441 aaaaccaagg gacgcaaatt cgaggaagaa tcccatcctt atcgcacaca ctatcaaagg
     7501 gatcgcgata gaataattca ttctactgct ttccggcgca tggaatacaa aacccaggta
     7561 tttgttaatc atgagggaga ccattatcgc acgcgcttga cccatactat agaagtagcc
     7621 caaatctctc gctccattgc ccgggcttta aaattaaatg aggatttagc cgaagccatc
     7681 gctctggtgc atgatttggg acatacaccc tttggtcatt ccggggagga agcgttaaac
     7741 gagataatgg tgaattatgg tggattcgag cacaatcgtc agagtttgag agtagtggat
     7801 gtcttagaag agaaatatcc ccagtttccc gggataaatc tcaccttcga agtgagagaa
     7861 gggataatga aacacgagac catctatgac cagcctattc cagttgaatt caaccctaat
     7921 acggcgccca ctttagagtg ccagatagtt aatctggcag acgaaatcgc ttacaactgc
     7981 cacgacattg atgatgggtt agcttccaac atcctcaaag aagaggagtt gaatcaagtg
     8041 gatctgtggg cacaattgcg tcaagaagta accaaagaat tcagcaactt gaacgctttt
     8101 caactgcgca accaggtggt gaggaagatg atcgattcgc aggtctcgga cctgataaac
     8161 gagaccactc gtcggttggg caaatatcat ataaatagtt tggaggatgt acgcaatttt
     8221 gaagaggggc ttttgagttt cagtcaggag atgaaaaaga aaaatagcca gctcaagatg
     8281 ttcctctttg ataatatgta ccggcattat cgcatgataa ggatggcgga taaagccaag
     8341 cgcatcacaa gacaactgtt cgatgtttat ctgaacgatg ttgaccagct tcctcccacc
     8401 acccgcagcc gggtgagaga gggaaatcgg atgcaagcca tttgtgacta catcgccggc
     8461 atgaccgacc gttatgcact tctggaatac aaaaaactct ttgacccgat ggagagggtg
     8521 tgagctggag tcctgagatg tccaatctac tcgcttttta ctttttattt atgagcttga
     8581 aataaatagg gggtgaggga atggtagtta agtactttaa attcgcagcg ttgacgatag
     8641 tcacattgtt gcccattctg tttatcccag gaatcaatta cgccagaaat tttgtcttcg
     8701 gctcctatat gcagcctgtg acctggtatc ccaaatacaa ataccctgag atgtcagaca
     8761 gtttgaagtt taacataata ggcgatgggt acttatatac gggaaatgca ttagattcta
     8821 tcaaggaagc ccatttaaag gttataattc agaattgtga aatgggggat tacccctgct
     8881 attattcttc agggcactac actgtttggg aagccgaagg agagctgcca gaatatcact
     8941 ttaccctgac ccacaatcat ggaattggtt ccgcagttta cgacaatgcg gcttctaatt
     9001 tgtatgcttg gaaagttgat aagaatctgc ctggaagctt gcctagtatc attcaagaag
     9061 gacccggtta cagacaggaa atgccagtga atgacaattc gagtgacaga aactatactg
     9121 tagagtatag aatgagatta gacgaagtgg tcacagattc aacccaatgg gatgaccctg
     9181 tcgcagtttt aatagtgcgc agagatagcg ccactgatac tttaggagcg atagacaccg
     9241 ttcgagttag ggatttctct gaaggtataa gtgaaccaga tacactctac cattctttca
     9301 ttttgaaata tgagcccgtt gctgtttact tgttgaattt agagatttat tggatggcgg
     9361 cacataatct tttgatagat aaagttacag tttatgacga tagagggttt gaacttttta
     9421 ataatccttc tgccgctgaa agcatcaaag cttatgttag acaatcttgg gttgacatga
     9481 cagtttacag atggtacctg aaagatgaag cgacattttc cgtagattgt tttatgcctt
     9541 acaggtttgt ggatagctta cttgcagaag tcaatcaaga aagaagagca gttacatgtg
     9601 tttttttgag agatgacagg tatcctagtg accccaatat gatagctgaa tttgttaaaa
     9661 ggacaaatcc gaggcaagtc aatatcgaaa attatgttct aaatggtcat aatgactgtg
     9721 gaaacacata tgcgccatta tattatacaa cacactcagg cgaaggttac tggccaccag
     9781 attccatggg gctacaaatt agtcttgacg gatttactca tactttagag gagttaaacc
     9841 agtcgatcga tattaatagt atcgatctat gggtgtccgt ccaagcgttt aggtggcgct
     9901 ggtgttgcgg gtgggtgaga cgaactccga cacctgaaga attgaggtgc atgtcgaatt
     9961 tagcattgat ttatggggct aaaggcatct tgtattgggt ttatttttcc actgatactg
    10021 ttagtggggg tggtccttgc ccggactcaa caatcattca atggcatggg ttggtaaact
    10081 ggtatggtta tgaaaaaaca gaaaattggt atgagataaa aaataatatt ggtcctttca
    10141 tagataaact tggagacacc ttttgtagcc taaagtggca aggcgcctgt tcggatgata
    10201 ttgtacgtta tcttgttttg cggaatggtc agccgagcta cctcgacagc attagatcgc
    10261 ctgggcatga accgcattgg gtgcaggtcg gattctttga gaatcaaact gctgacacca
    10321 gttattttat gttggtgaac cgtgaatgtt tggtaaccga aggtgattct tttgatgttt
    10381 ttgtcaacaa atctggtgga tattatcgaa taagagatat gtatagtgac agtatagtcg
    10441 gaatagtaaa aggaaaaggg gattatttta aagcttattt gggaccggga gaaggcaaac
    10501 ttcttagatt agaaaaggta gttagcggcg atgtcaatgg aggtggaggg cggtccatta
    10561 cggatgttgt ctatctgata acttatcttt tcaagggtgg tcctccacca aaatgcccac
    10621 cccagccata tctttcctgt gcagatgtca actgtgatgg ccttgtaaat gttactgatg
    10681 taatctatct tatcaattat cttttcaaat tcgggccacc accggggtgt tagaatataa
    10741 aaagctcttt gacccgatgg agagggtgtg aggtggctag aaagaaatat aacattagaa
    10801 aggaggggtt tctatgctaa gacgttttca tctaaaagct tggttttgtc tgttctttct
    10861 tattctttgg gcgttgccag ttgtcgcgca agatataaat atgaacaaga acaactggag
    10921 gcattacacg ccgccggaaa acatatacaa cgagaccgtg aacggcatag ccacagacga
    10981 ttctgggaat acctatgtga ctggatggag ccacaaccaa gagacaggca atgattacgt
    11041 aaccattaag tataatcaac atggagatga agtctgggta agaaaataca atggatcagc
    11101 aagtttgggc gatcgggcta atgccttagc catagataaa ttgggtaata tctatgtgac
    11161 tggatggagc agggataatg ggacgtggga tgactatgtt accataaaat attattccaa
    11221 tggagatact gcctggacca gaagatataa tggacctgga aactggcatg acggagcttg
    11281 ggatatagcc acagaccgat ctggcaacgt ctacgtgacc ggatggagtt ggggcgagga
    11341 gacgaaattc gactacgcta ccattaagta tgataaaaat ggtaaagagc tttgggtgag
    11401 aagatacaat ggtccaggaa actccgagga tagggctctt aatatagcga tagacaagaa
    11461 cggcaatgct tgtgttagcg gaatcagtct ggtgcctggg aagtattggg actatgtcac
    11521 tataaagtac aagccgaatg gagattgtct gtgggtgaaa aagtacactc gtcaaggcaa
    11581 cccaccagtc ggaattttgt gaagaaacac tcgccgtctt gaagtaaaaa agctttttga
    11641 cccgatggag agggtgtgag ataaataggg ggtgcgaaaa tgtcaagatt caagttttgg
    11701 ttattgcgcc tttggcttct tttagtattc ttttctttct tctttacttc cgcagttgcc
    11761 tttgaaacca aatttcatcg ttgtgtcctc ggtcgtctct tcacatggcc agatgtaagg
    11821 ttttatcctt atttccatga aaccaaaagc catggtttca attgcattag tacccgggat
    11881 tttactctcg aggattacaa ttcagcttcg gttgatactc ttaacctgat cgacgagagc
    11941 tttggcggta ccccaccttt agtggcgcaa tacagccgct acgaagccga tcagcataat
    12001 ttaagggatt attggcagga cacgtctggg attcaaatca gatttctgag cacagatggg
    12061 caacataccg ggacaggagg acaagtgagc gatgcgcaag tggccaatgg ttatgtatgg
    12121 tttgtttcaa aaaacagccc agctgaccat cgaggatata ttctgttcgg gctgtggaat
    12181 gaaaagcccg acgtgcaaaa caatacgtgg cacatagact acagtcgtga aatgcataat
    12241 ccctgggata gtctaattta ctatgcacgt tttaacttga aagtacccgg tggtggctat
    12301 tctccgcttg atacagttgc aaagattgaa gcgcatctta tgaacttaac acaagataga
    12361 ctggtagcgg attccctcct tctttaccaa gatttcgatc cagatcaata taaaatcttt
    12421 gagctttcct tttggaaaca tccagaaaac gccaatgact cgttggatta taaaatttat
    12481 tggtataaga agaccgacct ttatattgac tatgtggaag tgtacgactg ctactatgat
    12541 accttggtga atcacaaacc catgtatcaa aatagaatca aatctgaaat cgcgccattt
    12601 gaaaatcaat atcatgggac ctctctattt cgatggtacc ttaaagacga accggaatac
    12661 aaatattaca aagcaaatag ggaaatcagc gatttacttg actcactggg ttatgcgcca
    12721 ggaattcagg cagtagggca tgagtatatt ccaaaaagat ttgccgacga gatacagccg
    12781 catgaaatat gttgggatga ttacattata gctacatggg ttgttccaca ccttcttcag
    12841 taccagctaa atcagcttac cttccgtctc ggacaagtgg ctgcggctgc acgcgaacat
    12901 aatgtcgaat ggtggtacgt ggcccaaact tacagggaat ccgattcgtt agccagatat
    12961 ccacacaaca gcgagctaag ggctacagtc tatttgtcat tggcttatgg cgccaaagga
    13021 atggggtact ttcggtacag cagtgcccca tggtggactc ccgagactcc aatcggacag
    13081 agcggtttga tgtggtggaa taaccttatc tcagattggg tccctgttga aacctattcg
    13141 aatccgaatc cttactcgaa ccgacctgag actttgctaa cggaggtcgt ccggatcaat
    13201 ttctggcttg attccctcgg cacaacgctg cggagtctcg actggattga tgcttgcgtg
    13261 gacagttcct acaacaacaa tgacctcctt ggttgtggtg ccggctacat cgacagcatc
    13321 cgctcgcata attctgcgga tgagccgcat tgggtgcagg tggggttctt tgagaatcaa
    13381 acttctgaca ccagttattt tatgttggtt aaccgcgaat gtttagtaac tgaaggagca
    13441 aactatgacg tctttgttaa caaatctggt ggccgttatc gaataagaga tatgtatgct
    13501 gatactattg tgggaatagt aaatggaacc ggagaccact ttacagttta cttgggacca
    13561 ggtgagggta agctttttag attggagccc ataccaaaga taaaaaatgt gcccgcagac
    13621 tatgccacca ttcaatcagc tatcaatgcg acttgggatg gagatacggt tttggtggct
    13681 cccggaactt attatgagaa tattaatttc cagcgcaaag ctattactgt ggcaagcgac
    13741 ttcatcttcg atcacaaccc cgctacaatt caagctactg taattaatgg cagtgcacac
    13801 ctcagtagcg tggtgagctt tggctctggc gagagctcga cctctgttct taaaggactc
    13861 actattacag gtggcacagg cacttcatgg agcggtcagg gaaggaaagt gtggttaggt
    13921 ggaggcattt attgcacgta ttcatcgcct tctataacca acaatttgat cattgataac
    13981 cacatacctc actattcggg actgggatat caatggggtg gtggcatcgg ttgtcagggg
    14041 ggatcgagcc ccattatttc caataatttg attctaagca atagttgtgc taatgacagt
    14101 ttaggtggtg gaatatattt tgctggagga aggcctgtta ttgtaaacaa tactttgtcg
    14161 ggaaataccg gagggggaat aacattttct acaactgatt atgctgattc ctttgttatt
    14221 agcaacaaca ttatcgctaa tagcacaagt ggctggggga tacgttctga agttgttggg
    14281 ccaaacacta atggtatttc atataatgat gtttggaaca atgcctttgg cagttttatg
    14341 ggttgtcctt ctggagtggg tgataccacc tggggaacta atgctaatgg aactccttgc
    14401 gacagtttcc acaatattat cagagatcct atgtttgtga atccagatgc cgattaccat
    14461 ttgcagcggg gttctgtctg tgttgatgct ggagacaata atgcaatata catgcctaac
    14521 ttggattttg acggcaaacc acgcatcgca gggaagcatg tggatatggg tgcatatgaa
    14581 aacgcaggct tcatttgtgg ggatgcaaac ggagatgggc aaatcactgt ttcagatgtg
    14641 gtttatttga taaactatct attcaaagga ggccctcctc cggaatgccc accccagccg
    14701 tacctttcct gtggagatgc caattgtgat ggtaaagtaa ctgtctctga cgtaatctat
    14761 ttaattaatt atcttttcaa aggcggtcca ccaccggggt gttaaaaaat gtaggggcaa
    14821 ccccccgcgg ttgcccaaac aaaccaaggg caggcacaga ggcctgcccc tacaatttgt
    14881 atccgtaggg gtgggaattc attcgtcccc tattttattt ttcttccatc aatttcaatc
    14941 cgctgacatg tcccccaaga tttctgttga tttttctttt aatttttgta atatttcttt
    15001 ttagttaaaa aacttttcac gcggaggata aagatgatag ttattcccaa gaggatcttc
    15061 ttgaccaaag gagttggaaa gcataaagac aagttaaata gctttgagtt agcgctgcgc
    15121 aatgctggca ttgcccgttt caatctggtt ggcgtctcca gtatctttcc cccgagttgt
    15181 aaactgatct cgcgaaagat gggcttggag tttctggagg atgggcagat attatatagc
    15241 gtgatgagcc gtaactccac caacgagccg aaccgtttga tttctgcctc agtcgggctt
    15301 gccataccca acgacagaaa tcaatacgga tatctttccg aacatgaagc ctatgggtgg
    15361 actgaaagga aagccgggga ttatgccgag gatttagccg ccaccatgct ggccacaact
    15421 ttaggattgg attttgacgt ggacaagagt tatgaccaaa gaaagcagat ttggaaaata
    15481 tccggtaaga tcgtccgcac caccaacatg acccaatcag ttgaagggga caagaacggg
    15541 ctctggacca cagttgtggc agcagccata ttcgttaccg tctaatttgt gggggagaaa
    15601 aatgaaaaaa tacctacatt ctccaactcg cccttttgag gttagatcgg actccaaagc
    15661 ggatgatata cttgaacgta tgggcagaat ctcctttcag ggaagaaatt tatccattgc
    15721 cttatctata tggaaagaga tgttgaaggg aaataatgtc atctttttgg ggttagccgg
    15781 ggctatggtc ccagctggaa tgagaaagat aatctccttt ttaattaaac aaagatacat
    15841 tgattgctta gtttccaccg gagcaaatct ttatcatgat ttacacgaat ctctgggtaa
    15901 gtatcattgg attggttcgt gtatggtgga tgattgcgat ttacagaaaa agaaaattga
    15961 ccgcatatac gatacttttg cctcagacag ggagtttcgc caggatgaca attttattat
    16021 agatttcgtt aaaactttag acttgaagcg cacctacacc acgcgagagt tcctctatct
    16081 attgggtaga aagctgtccg tggcagggga gaccgaggga atcttaagct cggcttataa
    16141 ggcgcgcgtt cctattttct gtccggcttt aggagattcc tccatcggga ttgctatggt
    16201 ctggtatttg gttcaaagcg gcataagccc tacgtttgac atagtgcgcg atgcggctga
    16261 aatctcccat atcacccaga aagccaaatc aactgcagtg atatatgttg gcggaggaac
    16321 tcccaagaac tacatccagc agtctgaggt gattgcagct gaattacgca agggaagaga
    16381 gattgggcat agctacgcta ttcagattac aactgattct ccccagtggg gaggcctttc
    16441 cggatgcacc tttgcggagg ctcaatcttg gggaaagata gggcttaggg ccaagaaggt
    16501 ggatttgcat gcagatgcga ccatagtctt gcccattttg gttacagctt tgcatcagag
    16561 tctgggtaag tcctttaagc gcaagcatac tcccagcttt gatttcaaag gcaaatttga
    16621 tttcggttag aatgaccttt taataccaac cgaaagtccc cacttgttaa ggtggggatg
    16681 aagagtagtt tgtgagtttt tgtgtcattc ctgtccccgt aggggataaa ctccggcaga
    16741 gcctgtcccg tactggatac gggaaatcca gaaaaggaaa gcgagtatgg atccccgatt
    16801 aaaccctcgg ggatgacaaa acacagtagg ggcaattcat gtggcacatc ccgttaaaag
    16861 cggggaattg cccctacaaa ctcaggacaa aagaaaattg gataaagaca gtttttccaa
    16921 gcgaaacttt ttagccattt cagaggagtt ctccaactta gagaattccg aatttgttat
    16981 cctgccagtt ccctacgagc agaccactac ctataaggcc ggcacacgtg agggtcccct
    17041 tgccatcatc aacgcctcag ctcaggtgga gctatatgac gaagagttag ggcttgaatc
    17101 ttatagaaaa ggtatctgta ctttggataa tctaaacgtt acctcacttg gtccgaaacg
    17161 tatgaacgag ataatctacg aggcatcaaa ggagttaatc catctcaaaa agaaagtggt
    17221 gatgttaggt ggagaacaca gcataagttg gggtttagtc aaagcctata gggaaagata
    17281 tcccgacttg tctgttcttc agctggatgc tcatgctgac ttgcgggacg aatatcagga
    17341 aaacaagttc aaccacgctt gtataatgcg aagaataaga gagcttgttc cgtccgttca
    17401 agtgggtatc aggaatttga gccaggagga ggcggaatat atcaaaactc aaaaaaagtt
    17461 gcctatcttc tatgcccaga aaatgatttc ctcggatgag tggatggacc aagccatagt
    17521 tttactttca gatgaagtgt atgtaactat tgatttggac tttttagatc cgtccattat
    17581 gccagcagtg ggaactccgg agccgggtgg tatcctgtgg tatcccaccc tgaacttctt
    17641 aaagaagctg gcaagacgaa agaaaatagt tggatttgat atggttgaac tggcaccctt
    17701 gccaggtatg gtcgcgcctg attttctagc agccaaactg gtttataaat tgattggtta
    17761 tctcgtagag caaaagaaga tttaactcat agtatgggct tgttggagaa gtgagaagct
    17821 tgtcattgcg agcccgcagg gcaaagcaat ctctatacgg cttgaaagac aacaaccaaa
    17881 gattgcttcg tcgtcccgcc tttggcggga ctcctcgcaa tgaccgaaaa aagcgttggg
    17941 caacagtctc atataataac tcaattttta atacagtaag ggtgggaaat tttcccaccc
    18001 tcaatagtat ataacttctt gatatgagct tatcctctat tttaagccaa gccagctctt
    18061 ctgttagctt taagcaactt gaaagggaaa tcgcctcaag taaaaaatgg attagtctct
    18121 gtgaagtggc tggttcaggc aaagcccttt tagcttctta cctgtatgat atctctccaa
    18181 catccatttt ggtgataact gacaaggcag acgaagcctc caccctggtt gaggatttag
    18241 agaatttcgt ggataagtca aagatttgtt atttccctgc ctgggaagtt ttgccttacg
    18301 agtggcgggt ccctcgcggg gagataatag gaacaaggct ggagactttg tatcaacttc
    18361 tatcaaacca gaaggttttg gtagtatctt ccctccgtgg ccttatggaa agaactctac
    18421 ctgtggccat cttgaaagag aatatctgga atctgaaagt gggtgacaaa ctccagttag
    18481 atactctggt tggcaagttg aatgagctgg gcttcattcg cgctgtccag gtggaggaag
    18541 tgggaagtta tagctcacgc ggaggtattc tggatgtctt tccttattcc tcatccagcc
    18601 ctttgagaat cgaattgttt gaagataagg tggagtcgat tcgggagttc agcatcctga
    18661 cccagcgctc cttcaagaaa gtcgattcag tactgattct gcccaagcgt gaagtcctaa
    18721 tcagcgatga gaagctggag gaatatcttt ctggcataga tgagcacaag gcagagagaa
    18781 tcagggaaaa gataagattc tacgatgagg ttccgggatt ggaatggctt gtctctcttt
    18841 tggacataag tcagtcaact cttttagatt atctgaatcc ccaagatatc gtcctctcgg
    18901 aagatatgag ttccctaaaa agtgaatacg acaacatctt acaggagaca gaaggacttt
    18961 gccagcaagc ggagaaaaaa ggagagatcg ttccttctcc tttgaaattg tggaacagct
    19021 gggaggaaat aaagaacaat atcgtccaac atcaactcct ggagatcaag gctttgagaa
    19081 gtaaagaagg cgagagcctt gagttcggaa tgacagagca ggagttcttt ggtggcaaga
    19141 tggatttcct gaaacaaaga attgaagaat acagaaaagg cagtaaaaag gtttatatca
    19201 tctgcgagaa cgctactcaa aaggaaagat tagctgagct tttggatgag ctctcctttg
    19261 aagttcattt cgagatagct atcctgcata aggggtttag ttttaatttg gctgaccttg
    19321 tggtcttaac cgagcaccag atcttctccc gctatttcag aaggagaaga aagaaaaggt
    19381 tcaaggaggg attagccctc tccagctata ccagtttgtc aattggcgat ttcgtggtgc
    19441 atgtggattt tggaattgga agatatatgg gcccagaaac cctcttggtg gataataaaa
    19501 aaagggaatg cctggaaata cgttaccagg acgaagataa gctttatgtg cctatagagg
    19561 aattcaatcg agtacagaaa tttgtgggca aggagggcgg tccagccctt tccaaattag
    19621 gcgggactgc ttgggagaga ttaaaaagca gaaccaagaa ggctatctgc gatatgacta
    19681 aggaattaat ttccctttat gctgaaagga aggccaagcc tggatttgct ttttcccctg
    19741 acacccgttg gcagaaagag ttggaatcat ctttcattta tgaggaaacc ccggaccagt
    19801 gggagaccat aaagcatgtg aaaaaggata tggaatcacc ccttcccatg gatcgtttgg
    19861 tatgcggaga tgtgggatat ggcaagaccg aagttgcggt cagagctgct ttcaaatgtg
    19921 taaccaatgg aaagcaagta gccgttttgg ttccgaccac catcctggcg caacagcatt
    19981 tggctacttt cattgagagg ttatccagtt ttccgataag agttgagttg ttatctcgat
    20041 tcaaaaccaa accggagcag aagaagatca tagaggatct gaaacagggg aaggtggata
    20101 ttgtcatagg aacacacaaa ctgcttcaga aggatgttgg attcaaggat ttgggactgt
    20161 tcatagtgga cgaggagcag aggttcggtg tggcgcataa agagaaactc aagaaactaa
    20221 gaacgcaggt agatgtttta accctgactg ctactccgat tcccagaact atgcagcttt
    20281 ctctgatggg gataagagat atgtccctaa tcaacacgcc gcccaaatac cgacttccca
    20341 tccagaccga gataggcaaa ttcgaagatg agctgatcgc tgacgccatc ttgaaggaga
    20401 tagacagagg aggtcagatc tatttcgttc ataatcgggt agagtccatc gattcgatat
    20461 atcgctactt aaaaaggatt ctgccgcagg tgcgtattgc catagctcat ggtcagatgg
    20521 aggaaggtgt tttagaagag gtgatgcttt cttttttgga tagaaaatat gacctgcttt
    20581 tagccacctc catcatagaa tccggcttag atatccccaa cgtcaacacc atcatcgtca
    20641 atcgggcaga taagttcggt ttggctcaac tttatcaact gcgtggaagg gtaggacggt
    20701 cgaaccaaaa agcctacgct tatcttctgg tcccaccttt tgcctctctg accgaatccg
    20761 ctcgcaagag gataaaggct ttagagcagt tcaccgagct gggttcgggt tttcatttag
    20821 ccatgcgcga tttggaaata agaggagcgg gcaatatcct gggtgcacag caacacggct
    20881 ttattcaaga gatcggtttt gacctttact gtaaactctt ggatgaagct gtaagggagc
    20941 ttaaaggcga aaagatagaa aaactgccag aagtgaaact cgatttcgat atggacttat
    21001 atattcctcc agattacatc tctgacagtg agcagcgggt ggagatttac cgtaagctgg
    21061 cttcagccca atctccgcag aaaatagagg agatggcatt ggagatggag gatagatttg
    21121 gtctcttacc acgagaagtg gagaatctct tggaggtggt gcgcataaag ctaaccgcag
    21181 ccaaaaaagg gatagtccat ctcggtttgc gaggggatat tttgaggatt ggtttctcaa
    21241 aagatagaaa aatagataaa aaacaaatag agggttttgc caaaaatatc gagtatcctt
    21301 tagagttctc ggtcagcggg gatttcacta tcaagcttga cttgaaaggt agagagcaca
    21361 agctggaggt ggtcaaaaac ctcttgccac acttttagag tataggtata ttggttgata
    21421 atgttcggaa cctcttaaat aaaaggagaa gcccagatga aaagaatagc gcttttgggt
    21481 ttgttgttgg ttttaatggt cttcgggttg gtggggtgtg ctaagcggga gaacaaggtc
    21541 gtagccaaaa tggagaacag ggagatcact ttgggggaac tggaaaatag gtttttcacg
    21601 cagatgaaaa tggcagccgc atccggcatg gacattccca aacaagagga tatcctggag
    21661 gatatgatca aaagagaggt cctgataatc tccgcctaca aacgagattt ggataaggac
    21721 caaaagatcg ataccttgct caaagagaga gaaaaaagcc tcttgatgga tgagcttttc
    21781 aaagtggaga tactgagcaa atcaaagcca actgactccg aagtcaagtc cttctacaac
    21841 aagttgaaag agaagataag ggccaggcat atactggtga aaaccaaaac ggaggcaggc
    21901 agcatttaca accaactgaa gaagggagcg aatttcgaac agttagccaa agagaaatcc
    21961 atcgacaagc agagcggaca gcagggtggc gacctgggat ttttcagttg gggcaatatg
    22021 gtggagcctt ttcaaaagac agctttcgct ttgaaaaaag gagagatctc caaaccagtg
    22081 aagacgcaat tcggctggca tatcattaaa ttggaggaga gggaaaaagc ggaacctcct
    22141 gatttccaaa gcaataagga ggcttatgcc caaaatctgg agaggatgaa acagaccgag
    22201 ctgataaatc aatatcttga agggttgaag aaaagagaga atttcaaagt ggattcgctc
    22261 accttcaata tactgctgac caaagcctcc cagctggata gcaccactgg agaaagaagg
    22321 gtagttggag ggatatccgt ggatattttc acccctcagg agcgtcaagc ccttttggtg
    22381 cagtatcgtg gcggaaagat gagcttggag gatttcgccc taaagttcaa tcttcttcct
    22441 gccaattcga gaatgaacgg atttggcgac cgagagggat tgaaggagct tgcctatcaa
    22501 atgcttctcc ccgatctgtt ggtggaggca gcccgtttgc ggggaataga taaaagcaaa
    22561 tcctatagga agaaattctt ggacattaag gaagcccaga tggcttcaaa gctggtgaat
    22621 gagttcatcc tggatacagt caaagtaacc ccagaggaag ttgagaaata ttaccaggaa
    22681 cataagaacc tttattttgt tcctcctcag gtgcatatcc aggagatatt gctcggctca
    22741 aaacaggagg cagacgaggt cctcaagaaa gtaaaatccg gagcggattt ctccaggttg
    22801 gctcaagaga agacattgcg cacttactct aaagataagg ccggcgattt gggatatata
    22861 caggaagctc aattcccgga gctttttaag gcaggcctca aattaagaaa aggagaagtg
    22921 ggcggaccta tatactccaa ggaatctatg ttaggagaag agtactcaat catcaagctc
    22981 ttggacaaaa aagaaggaaa acaaacgagt ttcgccgagg tgagggagta tgttggggaa
    23041 acggcactgc gggagaagca gaataaggtt ttggaggatt ggatagagaa agccaaggca
    23101 agcatgaaac ttgcggtcca caaagagctc ttgggagtgg ctaaaagcga cagtgtcaaa
    23161 acgggaagct aatatgaaaa ggggtttttg ggttttggct ctacttatga tttcctgctt
    23221 agctgggtgt ggtggtttgg gggacaagct ggaggataaa accgtcctgg ctaaagttgg
    23281 tgaccgcatc ttgacagctg aagatatgga aacggagatt ccggaagagt ataagagctc
    23341 agtgactttg gagcaga
//
LOCUS       DASWLN010000090        13038 bp    DNA     linear   ENV 20-FEB-2024
DEFINITION  MAG TPA_asm: Candidatus Zixiibacteriota bacterium isolate
            SMAG_U2949 k141_1052091, whole genome shotgun sequence.
ACCESSION   DASWLN010000090 DASWLN010000000
VERSION     DASWLN010000090.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37960594
            Sequence Read Archive: SRR10376091
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Candidatus Zixiibacteriota bacterium (soil metagenome)
  ORGANISM  Candidatus Zixiibacteriota bacterium
            Bacteria.
REFERENCE   1  (bases 1 to 13038)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 13038)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (26-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/12/2024 08:28:26
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 1,440
            CDSs (total)                      :: 1,416
            Genes (coding)                    :: 1,412
            CDSs (with protein)               :: 1,412
            Genes (RNA)                       :: 24
            rRNAs                             :: 1 (16S)
            partial rRNAs                     :: 1 (16S)
            tRNAs                             :: 21
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 4
            CDSs (without protein)            :: 4
            Pseudo Genes (ambiguous residues) :: 0 of 4
            Pseudo Genes (frameshifted)       :: 0 of 4
            Pseudo Genes (incomplete)         :: 3 of 4
            Pseudo Genes (internal stop)      :: 1 of 4
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..13038
                     /organism="Candidatus Zixiibacteriota bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_1052091"
                     /isolate="SMAG_U2949"
                     /isolation_source="soil"
                     /db_xref="taxon:2053527"
                     /environmental_sample
                     /country="USA: Oregon"
                     /lat_lon="42.99 N 123.63 W"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            complement(<1..400)
                     /locus_tag="VGB16_02960"
     CDS             complement(<1..400)
                     /locus_tag="VGB16_02960"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="MFS transporter"
                     /protein_id="HEX9916673.1"
                     /translation="MNSNDKKDKTLRFSFLDGIFASGMTGFTQDYFTPFILYLGATAR
                     QVGFLSALPNLFGALMQLKSADITEKLKSRKKVITSFVFLQALMLLPMVALSLLGEAK
                     IAIFIAIVTLFISLGAFVLPAWGSMMSDIIP"
     gene            complement(804..2141)
                     /locus_tag="VGB16_02965"
     CDS             complement(804..2141)
                     /locus_tag="VGB16_02965"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_014561274.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_process: GO:0006355 - regulation of DNA-templated
                     transcription [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="sigma-54 dependent transcriptional regulator"
                     /protein_id="HEX9916674.1"
                     /translation="MLKYRILVVDDEAAQREMLAGYLEKQGYKVSTAGSGREGLELCK
                     DTFFELALVDLKMPEMDGIQFLKELKKLSLDTEIIMMTAYGTMETAIQAMKEGAYDYI
                     SKPMDLEELRMNIEKILETQSILAENRFLKEQIDEKYKDLKIIGKSKNIKELLSTVAR
                     VAKTDSIVLIRGESGTGKELIARAIHTLSSRSENTFVPISCAALPETLLESELFGYEK
                     GAFTGADKRKEGRFEIADNGTLFLDEIGDISLQMQVKLLRVIETQSFERLGGKDTLKV
                     NVRIVTATNQNLEQKIKEGKFREDLYFRLNVISILIPPLRERKEDILPLVEHFIDKIN
                     KKCGKAIKGITPSAKDLILSYHWPGNVRELENVIERAVVLCRGEVIDKEDLGWMSFTQ
                     TQVPSDSLRDIEKNHISNVLKQNDWNLGKTAEILGIHRNTLRMKIEEYKLKPE"
     gene            complement(2149..4194)
                     /locus_tag="VGB16_02970"
     CDS             complement(2149..4194)
                     /locus_tag="VGB16_02970"
                     /inference="COORDINATES: protein motif:HMM:TIGR00229.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ATP-binding protein"
                     /protein_id="HEX9916675.1"
                     /translation="MKSPFSLKRILQVRLRYIILITLLLAGTLFFVTLFGIQKGKSDM
                     LRVMENEGTALLEGLVTASHNTLKANSLIEDLVEQRLEALSQVVEQGTGPKKIDSLFL
                     KKIVEENELLGIVILDKKGETISTSGVGKEVIYPSSDSVTILALNSLLEGKVDKIFLE
                     SEQGEGTPELKYLFAIRQNKEVIVILTSASYLEKFRKEIGIGYLIQKISQERGIDYIV
                     LQSPQGIILASRKVEKLLKIEEDPFLKEALLKGQADSRVTDFEGKEVLEVVKPFVSDE
                     MPSGIFRLGLSLEEYRYISQNYQRQMIIFTLVLFFLSLLFIGIVIANQNYSLLDRSYK
                     EMKTLTGNILEAINSAVVAVDGDNKVIIFNRAAEEVFSIHRDKAISQKYSDVFPQDDC
                     LLDETTKKGRRIHQVERKYKTFSGEEKHLIIGTSSFLDEQGKSRGAVGVLHDITELKR
                     FEEEAKKAERLSALGNLAAGVAHEIRNPLNAISIAAQRLKNEFSPTANQREYLSFTQT
                     MLDEIKRLNQIINQFLSLAKTQKLNLVAVDLKELLEDILQLLRIEAEEKGVVLKQELT
                     DLPKLEIDREEMKKAILNIILNAVQATRTGGEVRLSASQDRTSKEVIIEIKDTGEGIS
                     KENLSKLFQPYFSTKDKGAGLGLSIAYRIITDHKGKIDVESQEGMGTTFTIKLPYKG"
     gene            complement(4200..5453)
                     /locus_tag="VGB16_02975"
     CDS             complement(4200..5453)
                     /locus_tag="VGB16_02975"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_015757307.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0016020 - membrane [Evidence IEA];
                     GO_function: GO:0022857 - transmembrane transporter
                     activity [Evidence IEA];
                     GO_process: GO:0055085 - transmembrane transport [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="Nramp family divalent metal transporter"
                     /protein_id="HEX9916676.1"
                     /translation="MKKPLFPRSRWRSWLIFLALVGPGIITSNVDNDAGGITTYSLAG
                     ANFGYSLIWSLIPITFVLVLIQEMCARMGVVTGKGLSDLIREKFGVKVTFYLLFAVLL
                     TNLGNIFGEFAGIASSLEIFGISRYVSVPLGALFVWLLVVKGTYKSVEKVFLVACLFY
                     VTYIISGFMAKPQWSVVLSELSSPKIDFSIAPLGMLVGVIGTTIAPWMQFYLQAAVAE
                     KNIKVRDYKYSKADVIVGSFVVNIVAFFIIVVCAATLYKNGIKIETAKDAALSLAPLA
                     GKYCSALFAFGLLNASLFAASILPLSTAYTVCEGMGWENGVNKKFSEAPQFYGLYTLL
                     IVLGAGIILLPKVPLISVMYISQIINGIVLPAILVFMLIIINDKKIMKNYTNDKLLNI
                     VSWMACIILSGLSIFLVYSTVRSIF"
     gene            complement(5456..6727)
                     /locus_tag="VGB16_02980"
     CDS             complement(5456..6727)
                     /locus_tag="VGB16_02980"
                     /inference="COORDINATES: protein motif:HMM:NF015413.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="CBS domain-containing protein"
                     /protein_id="HEX9916677.1"
                     /translation="MNEKESKVFIFFSELLRRKLSDSQGKIIGKLVDLKVGLGELFPK
                     VVCLCIKIKNNRQTFTLDWERVDKIEEEKLVLKPESASLLLPLEVGEKEILLREEMLD
                     KQVVDTYGVRIERVNDLHLLIAGSELRLVHVDFGIRGILRRLGWVKSVDALTEWFFSY
                     KIPQKLLSWKYIQPLSRHAGNQALKLNLTHRKLDEIHPSDLADLLEDLDKHERLAVFK
                     SLGLEAAAETLEEVEPKLQRDLIETDTTERASDILEEMAPDEAADLLSELPPEKTEAM
                     IQGMETTKRKELEGLLAHPEGTAGSIMTLDYIALKENETAEEALRRFKRSDYHSESSS
                     YIYITDDQGKLKGVVTLRSLLICPSSTLLSTIMNRRVIKVKLEDKVEEVAEKFKKYNL
                     LMVPVVDGEKKIKGVITFHDVVDSIFPDFEG"
     gene            complement(6736..6900)
                     /locus_tag="VGB16_02985"
     CDS             complement(6736..6900)
                     /locus_tag="VGB16_02985"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_014403391.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF5668 domain-containing protein"
                     /protein_id="HEX9916678.1"
                     /translation="MSNHNGKGRGVFWGFLLLVVGILVLLHNLGYISGSIWRWWPVIL
                     IVIGLKKLIY"
     gene            complement(7188..8222)
                     /locus_tag="VGB16_02990"
     CDS             complement(7188..8222)
                     /locus_tag="VGB16_02990"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="histidinol-phosphatase"
                     /protein_id="HEX9916679.1"
                     /translation="MNWHEYIGAVHIHTSDSDGTKGVEEIVKLAQKVKLDFLLFSDHL
                     TLRSLYANLEGWYDSVLAIVGYEIHDQDNKNHYLIFNLNEVLPFGLSPAEYVREVKEK
                     GGIGIIAHPDETRNSLKNFPPYPWTDWEMDGFDGIEIWNEMSEWMENLTKTNQLKMIL
                     SPRKALKSPTDRILKKWDQINQKRKVVGIGAIDAHAFSYPLLGLFRITIFPYDVQFKS
                     IRTHILLTEPLSAEAIPAKKQILDALKECRAFVSNYRWGDAKGFSFVAQNSTKKVMIG
                     ENIEAKDEVKLFVQTPSDCYIKLLCNGKTVAETQSRMLEFTSKEKGNYRVEAYKNKKG
                     WIYSNHIRVN"
     gene            complement(8225..9589)
                     /locus_tag="VGB16_02995"
     CDS             complement(8225..9589)
                     /locus_tag="VGB16_02995"
                     /inference="COORDINATES: protein motif:HMM:NF023128.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="MFS transporter"
                     /protein_id="HEX9916680.1"
                     /translation="MPKHSVPRTQAVFSWGLYDFANTIFSMNIATMYFAQWIIVDHKQ
                     QDIWYGLTYSISMLFVAISMPVLGAVSDSYGRRKPFILFFTLGCVVVTFLMGLISQLI
                     SSLSLVIVSSLVLFGIANFFYEGGLVFYNALLPEVSTKANIGKVSGFGVALGYLGAIA
                     GLFLVKPFVEGNFFGWVLPKSLGGGREKSFIPTAIFFLIFSLPLFFCVKENLMAKADK
                     LNLDVKSAFARLWDGICNTRKYPGVLRFLIADYFIEDAIATVIIFMAVYAQLVMKMGD
                     EIKIWFFIVATTFAVIGSFLCGLVCDRIGPKKTLGIVVLGWVASLLVAIFTTNQVVFW
                     IIGPFIGIFLGSTWTSARPLLTSLAREDTLGQFFGLYALSGRAAAITGPVVWGGMVLL
                     FRQDNPLVELMVSFLNKLGISLSSSTLATIQYRFAVGTLTLLMLVGLIILIKVPDRFK
                     TGRK"
     gene            complement(9596..10021)
                     /locus_tag="VGB16_03000"
     CDS             complement(9596..10021)
                     /locus_tag="VGB16_03000"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEX9916681.1"
                     /translation="MIKSLGYKKSKFAFPLKVGLFCLAIWFIVNSQQTRADGFSSEKT
                     DSVNLSFYTNKDEWLGSDKVAHFTISLFLSAISYEMYHDNYYNDKNPSILFSGGFVFT
                     LGLGKEFYDRRRPNGRFSYKDLIADVAGISIGLILATNY"
     gene            complement(9999..10544)
                     /locus_tag="VGB16_03005"
     CDS             complement(9999..10544)
                     /locus_tag="VGB16_03005"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_012583840.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="HD domain-containing protein"
                     /protein_id="HEX9916682.1"
                     /translation="MNRQEGLDLVNSKVKTKNLLKHMLAVEAVMRRLAEHFGEDVEIW
                     GLTGLLHDLDYDETAKEPERHSLLTVEWLKPYNLDESITHAIKAHPKKVPAESKMDWA
                     LYATDPLTGLVVASALMHPTKKIKNLTAEFVLNRFKEKRFAAGASREDIRKCQELGVS
                     LEKFIELALEGMKRIDQELGL"
     gene            complement(10657..12498)
                     /locus_tag="VGB16_03010"
     CDS             complement(10657..12498)
                     /locus_tag="VGB16_03010"
                     /inference="COORDINATES: protein motif:HMM:NF019335.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="TonB-dependent receptor plug domain-containing
                     protein"
                     /protein_id="HEX9916683.1"
                     /translation="MNVPADSVQAKKKYQFISESGFDEKLLNLFSTRQSITQEEIDNS
                     IIRTWGDILNLRKGVGALDYSDFGQPEILQMGGSGERIGIVTDGFEYVNHAFRFPYIS
                     GLDLSWLPMENVEDIKIIEPGLAGIYNQDAPLGCLLTKSKEFNGEKVFSKALYQKAPY
                     NFRRTQLELGKDFTSRGAIFVTTGFKGYDGYLINNHLSSMHLTFSGKYILEENWNLRV
                     NASGFSAKRGLAHTEGFSLKPITEFGDDWRINLKLIHKATESSLSNFRISYYNMKQRF
                     NDPGFSVFREDIEEIMDLGLEHECFYREKHNLKLKGNWTYDRLDERSSIDHTQKTDLS
                     LADLFQLSPKVSFLFFIGMNKASRFDGRLSPMSGISYKINQNSKACFTLGSFWGFPSI
                     RELYLDSTSYTLSSSSGYEYTIEGKNDLKAQKSLVANLHYFLNKNNYSLGLSVHRSRL
                     ENSVLWENQDGLPYDGKWIPLNQDLNLYGAELDFLFNPRQNVKLFVSYAFQKAEEVKS
                     GFDQPFVPEHSFFCYLEYYKKLLKNEIQVGGRIENNLISSYYAAEEEKDKQENIDILN
                     LRITFKFLSFTCYYVVENLNNKSYKTFSSYPAPGRTQWWGFSWQFFD"
     gene            complement(12857..>13038)
                     /locus_tag="VGB16_03015"
     CDS             complement(12857..>13038)
                     /locus_tag="VGB16_03015"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=3
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEX9916684.1"
                     /translation="DYQRNKNSHYCTASKGEGEEYDGCEAIGVFPFPLPGRAGTLSLS
                     PKGREKLEKIAIKTF"
BASE COUNT         3667 a         3128 c         2366 g         3877 t
ORIGIN      
        1 ctggtattat atctgacatc atacttcccc aggcaggcag aacaaaagca cccagagaga
       61 tgaacagagt tactatggca atgaagattg cgatcttagc ttcacccaaa agagatagag
      121 ccaccatagg caataacatc aacgcttgca gaaaaacaaa tgaagtaatt accttcttcc
      181 ttgatttcag tttctccgtg atatctgcac ttttaagctg catcaaagct ccgaagagat
      241 taggcagggc actcaaaaac ccgacctgcc tggcagtcgc tcccagatat agtatgaaag
      301 gggtgaaata atcctgcgtg aacccggtca tcccgctggc aaaaatgcca tccagaaagg
      361 aaaaccgtaa agttttatct tttttgtcgt ttgagttcat ataaggaatt tattctattc
      421 ttttgccttt aaaagtcaag aggtttttga gggtggaaat gagtggttat tagatttgct
      481 ttgataaacg tagggacaaa aacactgttt tagtccctac aatattagaa acagcaggcg
      541 acccatctgg gtcgcctacg gtcaaaaatg tattgatcaa atgccgattt tctctcttca
      601 attgtaaacc gacgttgccc tcaacgtggg tgaatttccc gccttgaggg caaggcgggg
      661 atacaaatag caggcgaccc atccgggtcg cctacaacca agccaatgga ttcctgcgcg
      721 aattctgcgt cttgagaccg gagtttatcc cgcagaaatg cggggcagga atgacaacca
      781 aaacacatcg caaagaaaag aatctattcg ggcttaagct tatactcctc tatcttcatt
      841 ctcaacgtat tcctatgaat ccccaaaatc tcagctgtct ttcccaaatt ccaatcattt
      901 tgcttcaaaa catttgaaat atgattcttt tcaatatccc tcaaggaatc cgatgggact
      961 tgcgtttggg tgaagctcat ccatcccaaa tcctctttat cgattacttc cccacgacac
     1021 aaaaccactg cccgctctat tacattctcc agttccctca catttccagg ccagtgataa
     1081 ctcaagatta aatcctttgc agaaggggtt atgcctttta tcgccttgcc gcatttttta
     1141 tttatcttat ctatgaaatg ttcaaccaga ggcagaatat cctcttttct ttctcgtaat
     1201 ggaggaataa gaatcgaaat cacattcaat ctaaaatata aatcctctct gaattttcct
     1261 tctttgattt tctgttcaag gttctgattg gttgctgtta ctattctgac attgactttg
     1321 agtgtatcct ttccgcccaa tctctcaaaa ctttgtgttt ctatcaccct caaaagcttg
     1381 acctgcattt gaagtgaaat atcgccaatc tcatccaaaa ataatgttcc attgtcggcg
     1441 atttcaaacc gtccctcttt tcttttgtcc gcaccggtga aagccccttt ctcgtatccg
     1501 aaaagttcgg actccaacaa agtttcaggc aaagcggcac aggagattgg gacaaaagta
     1561 ttttctgacc ttgagcttaa agtatgaatg gctctagcta tcaattcctt tccggttcca
     1621 ctttcccctc taatcaagac aattgaatcc gtctttgcca ctcttgctac tgtgcttaaa
     1681 agctctttta tgtttttgct tttaccaata atctttaaat ctttgtattt ttcatctatc
     1741 tgttccttta agaatctatt ttcagccaag atgctctgag tctccagaat tttctctata
     1801 ttcattctta gttcttccaa atccatgggt ttgcttatgt aatcataagc accttctttc
     1861 attgcttgaa ttgcagtctc cattgtgcca taagcagtca tcatgatgat ttcagtatcc
     1921 aggcttagtt ttttcaactc ttttagaaat tgtattccgt ccatctctgg cattttcaaa
     1981 tccaccaggg ctaactcgaa aaaagtgtct ttacacaatt ctaacccttc cctgcctgaa
     2041 ccggcggtgc tgaccttgta tccttgtttt tccaagtaac ccgccaacat ctcccgctgt
     2101 gcggcttcat catctaccac taaaatgcga tatttgagca taaaatattt atcctttgta
     2161 aggtaacttt atagtaaagg ttgttcccat accctcttgg ctttccacat caatctttcc
     2221 tttgtggtca gttatgatac gataagcaat ggacaatccc aaaccggctc ctttatcttt
     2281 ggtgctgaaa tagggttgaa aaagcttgga cagattctct tttgagattc cctcaccagt
     2341 atctttgatt tcaattatca cttccttgct tgtcctgtcc tgacttgcgg ataagcgtac
     2401 ctctcctcca gttcgtgttg cctgaaccgc attcaagatg atattcagaa ttgccttctt
     2461 catttcctct ctatcgattt caagtttggg caaatccgta agctcctgct ttagaaccac
     2521 gcctttctct tcagcttcaa ttctgagcaa ttgaagaata tcctccagca gttcttttaa
     2581 atcaactgca accagattca atttctgggt tttggctaag ctcaagaatt gattgattat
     2641 ctgatttagt cttttaattt cgtccaacat agtttgggtg aaggaaagat attccctttg
     2701 attagctgtc ggagaaaatt catttttcaa tctctgtgcc gcaatggaaa ttgcattcaa
     2761 gggatttctt atttcatgcg ccactccagc tgccagattt cccagagctg acagtctttc
     2821 tgccttcttt gcttcttctt cgaacctctt cagctcggtt atatcgtgta aaacccccac
     2881 cgctcccctg cttttcccct gttcatccaa gaaagaggaa gtgcctatta ttagatgttt
     2941 ttcttctcca gaaaatgttt tgtattttct ttccacctgg tgtatccttc tgcccttttt
     3001 cgtagtctca tctaagaggc aatcgtcctg tggaaaaacg tcggaatatt tctggctgat
     3061 agctttatcc cgatgaatag aaaacacctc ttcagctgcc cggttgaaaa ttatgacttt
     3121 attatcacca tccactgcca ccactgcact gttgatagct tcgagtatgt ttccggttaa
     3181 ggttttcatc tctttataag acctgtccaa gagagagtaa ttctggttgg ctatcactat
     3241 ccctatgaaa agaagcgaca agaagaacaa aaccaaagtg aaaataatca tctgcctctg
     3301 gtagttttgg cttatgtatc tgtattcttc taaggaaaga cctaacctga aaatacctga
     3361 aggcatttca tctgaaacaa aaggcttaac cacctccaga acttccttcc cctcaaaatc
     3421 agttaccctg ctatccgcct gacctttcaa taatgcctct ttcaagaaag ggtcttcttc
     3481 aatttttaga agtttctcta ccttccttga agccaagatt atcccctgcg gtgactgcaa
     3541 gacgatataa tcaatacccc tctcttgact tatcttctgg ataagatacc caatcccgat
     3601 ctcctttctg aacttttcca gatatgaggc tgaggtcaat atcacgatga cttctttgtt
     3661 ttgtcttatg gcaaaaagat attttagctc aggcgtaccc tctccctgct cgctttccag
     3721 aaagatttta tcaactttgc cttccaacaa agaattcaaa gctaaaatgg ttacgctgtc
     3781 cgaagaagga taaatcacct cctttcctac gccactggtg gatattgttt cccccttctt
     3841 atctaaaata acaataccca gaagctcatt ttcctcaact atctttttca agaataagga
     3901 gtcgattttc tttggacccg ttccttgctc aacaacttgg gaaagcgctt ccagtctctg
     3961 ctccaccaaa tcctctataa gagaattggc tttcagggta ttgtggctgg cagtaaccaa
     4021 cccctccaaa agagctgtcc cctcattttc cataaccctc aacatatccg atttcccctt
     4081 ttggatgcca aagagagtca caaagaatag tgttcctgcc aaaagaagag tgataaggat
     4141 gatatatctt aatcttacct gcagaattct tttgagggag aaaggagatt tcatttgctt
     4201 tagaagattg acctgacagt gctataaacg agaaaaatgc tcaagccact taggattatg
     4261 caagccatcc aagagacgat atttaagagt ttgtcattgg tgtagttttt catgattttt
     4321 ttatcattta tgataatcaa cataaatact aaaatcgccg gcaaaactat tccatttatt
     4381 atttgggaga tatacattac cgaaattaag ggaactttgg gtaaaagaat tattcctgcg
     4441 cccagaacta tgagcaaagt ataaagtccg taaaattgag gagcttcaga gaacttcttg
     4501 ttcactccat tttcccatcc catcccttca cataccgtat aggcagtgga cagaggtaaa
     4561 atcgatgcgg cgaataagga ggcatttaaa agaccgaaag cgaaaagggc tgagcaatat
     4621 tttccagcca gaggagctaa agataaggcc gcgtccttgg cggtttctat ctttatccca
     4681 tttttataca gggtggcggc acagactaca attataaaga aggctacaat gttaaccacg
     4741 aaactgccca ctatcacatc tgctttagaa tacttgtaat ctcttacctt gatgttcttt
     4801 tcagccaccg cagcttgaag gtaaaactgc atccagggag caatggtggt tccaatcaca
     4861 ccaaccagca tccccagagg agcaatggag aaatctatct taggagagga taattcggac
     4921 aaaactactg accattgcgg cttagccata aaaccggata tgatgtaggt gacataaaac
     4981 aggcaagcta ctaaaaacac tttttccaca gatttgtatg ttcctttaac tactaataac
     5041 cagacgaaaa gggcaccaag gggaactgaa acatatctgg atatgccgaa tatctctaag
     5101 ctggaagcta tcccggcaaa ttcaccgaag atattaccca ggttagttaa tagaaccgca
     5161 aaaagtaaat aaaaggtaac ttttaccccg aatttttcgc gaatcaaatc agacagtccc
     5221 ttgcccgtta ccactcccat ccgcgcgcac atctcttgaa tgaggactaa aacaaaagtt
     5281 ataggaataa gcgaccagat taagctgtat ccgaaattag caccagccag agaataggtg
     5341 gtaattccgc cagcatcgtt atctacatta gaggtgatta tgccgggacc aaccagtgcc
     5401 aaaaatataa gccaactgcg ccagcgagat ctgggaaaca aaggcttctt catagttaac
     5461 cttcaaaatc ggggaatatc gaatctacca catcgtgaaa ggtgattacc cccttaatct
     5521 tcttctcccc gtccaccacc ggtaccataa gcagattata cttcttgaat ttctccgcca
     5581 cctcttcaac tttatcctcc agtttgacct taatcaccct tcggttcata atagtggaaa
     5641 gtaaggtgga agatggacaa ataagcaggc ttctcaaagt aacaactccc ttgagcttgc
     5701 cctggtcatc cgtgatgtaa atatacgaac tgctttccga atgataatca gaacgcttga
     5761 atctccttag ggcttcctct gcagtttcat tttctttcag ggctatgtaa tctaaggtca
     5821 ttatgctccc cgcggttcct tccgggtgcg ccagcaaccc ctccagctct tttctttttg
     5881 tggtttccat gccttgaatc attgcttctg tcttctccgg tggaagctca ctcaaaaggt
     5941 cagcggcttc atctggagcc atctcctcca gtatatcgct agcccgttca gtagtgtcgg
     6001 tttctattag gtccctttgc agttttggtt caacttcctc caatgtctca gctgcagctt
     6061 ctaaacccaa tgacttgaac acagccaacc tctcatgttt gtccaagtct tccaataaat
     6121 ctgctaaatc cgaagggtga atttcatcca gctttctatg agtcaaattc aacttcaggg
     6181 cttgattgcc agcatgtctg gaaaggggtt gaatatattt ccaggaaagt aatttctgtg
     6241 gtatcttata ggagaagaac cattcggtta aagcatccac agacttgacc cagccaagcc
     6301 gacgcaatat tccccttatt ccaaagtcaa cgtgaaccag tcttaattca cttccagcta
     6361 tgagtagatg caggtcattc accctttcaa tcctgacgcc ataagtatca actacctgct
     6421 tatccagcat ctcctcccta aggagtattt ccttctctcc aacttccaga ggtagaagca
     6481 agcttgcgct ttccggttta agcacaagtt tttcttcttc aattttatcc accctctccc
     6541 aatccagagt gaacgtttgt ctattgtttt tgattttgat gcaaaggcac acaactttcg
     6601 gaaaaagctc acccagaccg accttgaggt caaccagctt tccgataatt tttccttgtg
     6661 aatcggatag ttttctcctc aacaattcgg agaagaatat gaagactttg ctctcttttt
     6721 cgttcattgt ttttattaat agattagttt ctttaatcca attacaatta aaatcacagg
     6781 ccaccagcgc cagatgcttc cgctaatata acccagattg tgcaagagaa ccaatatccc
     6841 gacgaccaaa agcaaaaacc cccagaaaac ccctctgccc tttccattat gattcgacat
     6901 ttttgcctcc ttgattattc atcaatcggg tgacgctgtt caagaagtgt cattgcgagg
     6961 agtcccgcat tttgcgagac gacgaagtcc cgccattggc gggaccacgc tccctccctt
     7021 cgacttggct cagggacggt gagcaaagtc gaaccgtacg gtcgctcgca atgacaaaaa
     7081 gacgaaagac agcgtttcta gacaaccttt ttctatcttt tatcctttcg ggaacggtgc
     7141 cgtaaaccat gcggtttatg gcggtgcaac cgttccctac ccggttatca atttacccta
     7201 atatgatttg aataaatcca gcccttttta tttttataag cctccaccct gtaattccct
     7261 ttctctttac ttgtgaattc gagcatacgg ctttgggttt cagctactgt ctttccatta
     7321 catagcaatt taatataaca atccgaaggg gtttggacaa ataatttaac ttcatctttt
     7381 gcttctatat tttcgcctat cataaccttc ttggtggaat tctgggcgac aaaagaaaat
     7441 cctttggcat caccccaacg ataattcgaa acaaaagccc gacattcctt caaagcatcc
     7501 agaatttgct ttttggctgg aatggcttca gcggaaagcg gctcagttaa aagaatatga
     7561 gttctgattg acttgaactg cacatcataa ggaaaaatag ttatcctaaa aaggcccagc
     7621 aagggatatg aaaaagcgtg tgcatctatg gctccaattc ccaccacttt tcttttttga
     7681 ttgatttggt cccatttttt caagattcta tccgttgggc ttttgagtgc ttttcttggt
     7741 gacaaaatca ttttcagctg attggttttg gtcagatttt ccatccattc tgacatttca
     7801 ttccagattt caattccgtc aaaaccatcc atttcccaat cagtccaggg ataaggagga
     7861 aagtttttca gactgtttct cgtttcatcc ggatgggcaa tgattcctat tcctcctttt
     7921 tccttcactt ctctgacata ctccgcaggt gacaggccaa agggtaaaac ctcattcaaa
     7981 ttgaaaatca gataatgatt cttattgtct tggtcgtgta tctcgtaacc gactattgcc
     8041 agcacgctat cataccaacc ctctaaatta gcataaagcg accttaaggt cagatggtca
     8101 ctgaaaagca gaaaatccaa ttttaccttc tgagctaact tcacaatttc ctcgacccct
     8161 tttgtgccat ccgaatccga tgtgtggata tgcactgccc ctatgtattc atgccagttc
     8221 ataattattt tcgtcctgtt ttgaatctat ccggcacttt gattaaaata atcagcccaa
     8281 ccaacatcaa caaagttaat gtccccactg cgaaacgata ttgtatagtc gccagggtgc
     8341 ttgaagacaa actgatgccg agcttgttca aaaacgagac catcagctca accaagggat
     8401 tgtcttgtct aaataagaga accatccctc cccagacaac aggaccagtg atagctgcgg
     8461 ctctgccgga aagggcataa agtccgaaga actgacccag ggtatcttcc cgggccagag
     8521 aggtcaaaag gggccgggca gaagtccagg tagagccgag aaatattcca ataaagggtc
     8581 cgatgatcca gaatacaact tgattggtgg taaaaatagc caccaaaaga gatgccaccc
     8641 aacccaaaac aactataccc aaagtcttct tcggaccaat cctgtcacat accaagccac
     8701 ataaaaacga accaattact gcaaaagtgg tagctacaat aaaaaaccag attttgattt
     8761 catcccccat cttcatcact aattgggcat aaactgccat aaagattata accgtggcta
     8821 tggcatcctc aataaagtaa tctgcaatca aaaatctcaa gacaccggga tatttcctgg
     8881 tgttgcatat tccatcccat aatctggcga aagctgattt cacatccaag tttaatttgt
     8941 ccgctttagc catcaagttt tccttcacgc aaaagaaaag cgggaggctg aatatcaaaa
     9001 agaaaatcgc ggttggtatg aaagatttct ctcttcctcc tcccaagctt ttcggaagaa
     9061 cccagccgaa aaaattacct tccacaaaag gtttaaccaa aaatagtcct gcgatcgccc
     9121 ctaaataacc caaagctaca ccgaagccgg atacttttcc gatattggct ttggtgctga
     9181 cttctggcaa aagggcgttg taaaaaacca atcccccttc ataaaagaaa ttggctatac
     9241 caaacaaaac taagcttgag acgatgacca aacttagact tgaaatcagc tggctgataa
     9301 gccccatcaa aaacgtgaca accacacaac ccagagtgaa aaacagaata aacggctttc
     9361 tccttccgta actgtcagaa actgccccta aaactggcat ggagattgcc acaaacaaca
     9421 tcgagattga ataggttaag ccataccaga tatcttgctg cttgtggtca acaattatcc
     9481 actgggcaaa atacatagtg gcgatattca tggaaaagat agtattggcg aaatcatata
     9541 aaccccagct gaagacagct tgggttctgg gaactgaatg ttttggcatt gtgttctagt
     9601 agttcgtggc taagattaaa cctatgctga ttccggctac gtccgctatt aagtccttat
     9661 aactgaacct gccattcggc cttctcctgt cgtaaaactc cttacctaat cccaaggtga
     9721 aaacaaatcc tcccgaaaat agaatagatg ggtttttatc gttataataa ttatcatgat
     9781 acatctcgta actaattgca gagagaaaca ggcttattgt aaaatgggca accttatcgc
     9841 tacccaacca ttcatcttta ttggtgtaaa atgaaaggtt aactgaatca gttttttcgg
     9901 aggagaatcc atctgctctt gtttgttggg aattgactat aaaccaaata gcaagacaaa
     9961 acaaccccac tttgagcggg aacgcaaatt tcgatttctt ataacccaag ctcttgatca
    10021 atccttttca tcccctctaa agccaattcg atgaactttt ccaaggaaac tccgagttct
    10081 tgacatttcc gtatatcttc acgacttgct ccagctgcaa atctcttttc tttaaatcta
    10141 ttcaggacaa attctgctgt caaattcttg atcttcttgg tgggatgcat caaagcagag
    10201 gcgacgacca ggccagtcaa ggggtcggtg gcataaagcg cccagtccat tttgctttca
    10261 gctggaactt tcttgggatg ggctttgatg gcatgggtta tgctttcatc caaattgtac
    10321 ggcttgagcc attcgacagt taaaagtgaa tgcctttccg gctccttggc ggtttcgtcg
    10381 taatccagat cgtgcagaag cccggtcaaa ccccaaatct caacatcctc accaaaatgc
    10441 tccgctaacc ttctcatcac tgcctcaact gccagcatat gcttgagcag atttttcgtt
    10501 ttcaccttgg aattgaccaa gtccaatcct tcttgcctat tcataacact ctctcaatcg
    10561 ggtaagtaat ggctgcgcca ttacttttgc tgttttttat cttttcggga acggtgcaac
    10621 cgttccctac ccgctatcca tattattctc ctttaatcaa tcgaagaact gccagctgaa
    10681 tccccaccac tgagtgcggc ctggggcagg ataactcgag aaagtcttgt agctcttatt
    10741 gttcagattc tccaccacgt agtagcaggt gaagctcagg aatttgaatg tgattctcag
    10801 attgagaata tcaatatttt cctgtttatc cttctcttct tctgcagcat aatagctgga
    10861 aatcaagtta ttttctattc ttccacccac ctgaatctca ttttttagta gttttttgta
    10921 atattccaga tagcaaaaaa aggaatgctc cggaacaaaa ggttggtcaa aaccactttt
    10981 tacttcctcg gctttctgaa aagcatagga aacaaatagt ttcacgttct gtctgggatt
    11041 gaagagaaaa tcgagttctg ccccatataa atttaaatcc tgattaagag gaatccattt
    11101 tccatcgtaa ggcaacccgt cttgattttc ccataggact gagttctcca atcggctacg
    11161 atgaacactc aaaccaagac tgtagttatt tttgttaaga aaataatgta aattcgccac
    11221 cagacttttt tgtgccttca aatcgttttt accctcaatc gtatattcat atcccgaaga
    11281 ggaacttagg gtataactgg ttgagtctaa ataaagctct cgaatgctcg gaaagcccca
    11341 aaagctcccc aaggtgaagc atgccttgga attctgattg atcttgtaac ttatccctga
    11401 cataggagac aaacggccat caaatcgact ggctttgttc attccgatga agaaaaggaa
    11461 actaactttc gggcttagct ggaataaatc ggctaaagac aaatctgttt tctgggtgtg
    11521 gtcaatcgag cttctctcat ctaatctgtc atatgtccaa ttacctttta atttaaggtt
    11581 atgtttttct ctatagaaac attcgtgctc caaacccaaa tccattatct cctcaatatc
    11641 ttctcgaaaa accgaaaagc ccgggtcgtt gaacctttgc ttcatattgt aataacttat
    11701 cctgaaattg gaaagtgagc tttccgttgc tttatgaatt aacttaagat tgattctcca
    11761 gtcatccccg aattcggtta tcggtttcaa actgaaacct tccgtatgag ctaatcctct
    11821 ctttgcgcta aaccccgaag cgttaactct taggttccag ttttcctcta aaatatactt
    11881 tccagaaaaa gtcaggtgca tagagctcaa atggttgttt atcaaataac catcatatcc
    11941 tttgaatcca gttgtgacaa atatcgctcc ccgagacgtg aaatcttttc caagttccag
    12001 ctgggtgcgc cgaaaattgt atggcgcttt ttgatacagg gctttggaga agaccttctc
    12061 tccattgaac tccttgctct tggtcaatag acaacctaaa ggggcatctt gattgtaaat
    12121 gcctgctaac cccggttcaa tgattttgat atcttccaca ttctccatag gaagccaact
    12181 aagatccaac ccactgatgt atggaaaacg aaaagcgtga tttacatact cgaaaccatc
    12241 tgtaactatt cctattcttt cgccagaccc tcccatctgc aatatctccg gttgtccaaa
    12301 atcactgtaa tccagagctc cgactccttt tcttaagttc aggatatcac cccaggtcct
    12361 gataatggaa ttatctatct cctcttgggt gatggattgt ctagtggaaa aaagattcaa
    12421 gagcttttcg tcaaagccgc tctctgaaat gaattggtat ttcttcttag cttggacgga
    12481 gtccgcagga acgttcacct cgaaagagga gtctggggga gaaacttgag cgtaaagcgc
    12541 attgaaaaac ccgaagaata aaataattaa ggcggtcttc ctaaacaatt ctcaccttcc
    12601 atttattact cgcctctgcc agaaaaccac gccatttatg gcgaggcgag cctcaccgaa
    12661 gtcccgcaga gcgggacgaa ggcgggcgaa gtttggctcg cctctcggcg aagccgaggg
    12721 cctttcatat ttcggaagtc ccgtgattta tcatggacgc ttcacttttt cctccggcat
    12781 gaaccatcac ggttcacgct tccgaataaa ccgacttaac ttctaaccag gagtatcccc
    12841 actttttgaa aacatatcag aatgtcttaa tggctatttt ctccagtttc tctctcccct
    12901 tgggggagag agatagagtg cctgcccggc cagggagggg gaaaggaaat accccaatag
    12961 cctcacagcc atcatactcc tcaccctcac cttttgaggc tgtgcaataa tgcgaatttt
    13021 tatttctttg gtaatctt
//
LOCUS       DASWLN010000091         8294 bp    DNA     linear   ENV 20-FEB-2024
DEFINITION  MAG TPA_asm: Candidatus Zixiibacteriota bacterium isolate
            SMAG_U2949 k141_970795, whole genome shotgun sequence.
ACCESSION   DASWLN010000091 DASWLN010000000
VERSION     DASWLN010000091.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37960594
            Sequence Read Archive: SRR10376091
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Candidatus Zixiibacteriota bacterium (soil metagenome)
  ORGANISM  Candidatus Zixiibacteriota bacterium
            Bacteria.
REFERENCE   1  (bases 1 to 8294)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 8294)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (26-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/12/2024 08:28:26
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 1,440
            CDSs (total)                      :: 1,416
            Genes (coding)                    :: 1,412
            CDSs (with protein)               :: 1,412
            Genes (RNA)                       :: 24
            rRNAs                             :: 1 (16S)
            partial rRNAs                     :: 1 (16S)
            tRNAs                             :: 21
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 4
            CDSs (without protein)            :: 4
            Pseudo Genes (ambiguous residues) :: 0 of 4
            Pseudo Genes (frameshifted)       :: 0 of 4
            Pseudo Genes (incomplete)         :: 3 of 4
            Pseudo Genes (internal stop)      :: 1 of 4
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..8294
                     /organism="Candidatus Zixiibacteriota bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_970795"
                     /isolate="SMAG_U2949"
                     /isolation_source="soil"
                     /db_xref="taxon:2053527"
                     /environmental_sample
                     /country="USA: Oregon"
                     /lat_lon="42.99 N 123.63 W"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            <1..819
                     /locus_tag="VGB16_03020"
     CDS             <1..819
                     /locus_tag="VGB16_03020"
                     /inference="COORDINATES: protein motif:HMM:NF012607.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="D-2-hydroxyacid dehydrogenase"
                     /protein_id="HEX9916685.1"
                     /translation="LSVKREIFLEAKKLKWIHLGVAGIDKTLFPEVLKSEVLVTSSKG
                     IHQVTVSEHVMGLVLAFAKGIAASIKAQPTKVWLQRELIDKRFNLEGKVLGIVGLGNI
                     GLELARKAKAFDMKVIALKNSVKKGEKYKNVDLLLGKEKLKELLSQSDFVVILLPLTK
                     ETYHLISQKELGWMKREAYLINVARGPIINEEDLIFALKNKTIKGAALDVVEKEPLDS
                     NSPLYDFENVIITPHIAGSMPDYYKRVGEIFKENLIRYLKKRPLLNLVDKKKGY"
     gene            820..1686
                     /gene="sucD"
                     /locus_tag="VGB16_03025"
     CDS             820..1686
                     /gene="sucD"
                     /locus_tag="VGB16_03025"
                     /EC_number="6.2.1.5"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_010879674.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003824 - catalytic activity [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="succinate--CoA ligase subunit alpha"
                     /protein_id="HEX9916686.1"
                     /translation="MSILVDKNSKIIVQGITGGAGTFHTKRMLEYGTNVVAGTSPGKG
                     GEKVLGLPVYDTVEECVRNHRADTSVIFLPAKFVMDAAIEAIYAGIKFIVVIPEHIPI
                     QDMLLIRKEAVKHSARVLGGNTAGIITPGEANLGIMPDIAFGKGRVGTVSRSGSLTYY
                     VADTLTRSGYGETTCVGLGGDPILGSTFDEILWLFEKDEKTKAVVMVGEIGGVYEERT
                     KDTISKMTKPVIAMIGGLYAPQGKRMGHAGAIVEGQMGTAQSKLKVLEEAGAHRANTF
                     LEIVEILKKLGV"
     gene            1714..2247
                     /locus_tag="VGB16_03030"
     CDS             1714..2247
                     /locus_tag="VGB16_03030"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEX9916687.1"
                     /translation="MFILLFLLLIGCAHRQKLAFIDLQKQKRVGNLVGNVYIIENAEI
                     DSLQGSTFSYPLKYVRNGKKTVVLIEGLRRPNFPYMPAKVDPPPFYCFLAEIEGLNYN
                     VGYDPREEEVLGLMPWLGGKRLEVEHCFLYFSAAQDAIIEGFVTITFSYGEGEQQGRM
                     LTGKFKGKRVERKDLFK"
     gene            2382..3374
                     /locus_tag="VGB16_03035"
     CDS             2382..3374
                     /locus_tag="VGB16_03035"
                     /inference="COORDINATES: protein motif:HMM:NF017864.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="asparaginase"
                     /protein_id="HEX9916688.1"
                     /translation="MSEIVAKVYRNDCVESVHCGSIVVVDSKGEIIYQLGDPYFKTYF
                     RSSAKPFQIIPVLKSGAIEKYNITEKEIAVMISSHSGEERHVKTVSSILKKIGLSEKN
                     LQCGTHPPLYLTELGILPRKEDKFSPLQHNFSGKHSGMLLLTVHKGWDVKTYLNPNHP
                     TQKIILKTIADICEYPLNKIRVGTDGCGAPIFYIPIYNMARGFAKLAISEDIYLKQVV
                     RCMSKYPEMVSGQNRFDLVLSQTLDKRGVCKGGAEGLHSTALLEKGWGVVVKISDGNH
                     RAIPPVALEALRQMGILKRQELAKIENWTNPKVKNYQGKIVGYIKADFKLKKGD"
     gene            3376..3915
                     /locus_tag="VGB16_03040"
     CDS             3376..3915
                     /locus_tag="VGB16_03040"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_006583057.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="HD domain-containing protein"
                     /protein_id="HEX9916689.1"
                     /translation="MRAKIIKLLPELRKIKDKELKEKVVLAWEMGIFEGGYKEGDLKK
                     ICFTLLIPECKVSLIQHTRAVTSTALAIAKDLTRNYGSKVKINYDYLIAGGLLHDVGK
                     LLEYCIDKNAVCKSKNGKFLRHAFSGTALAYVCGIPAEVLHIIAVHSKEGDTSPRSIE
                     AMIVHYADYIHFEALGGKL"
     gene            3943..5244
                     /locus_tag="VGB16_03045"
     CDS             3943..5244
                     /locus_tag="VGB16_03045"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011838402.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004834 - tryptophan synthase activity
                     [Evidence IEA];
                     GO_function: GO:0030170 - pyridoxal phosphate binding
                     [Evidence IEA];
                     GO_process: GO:0000162 - tryptophan biosynthetic process
                     [Evidence IEA];
                     GO_process: GO:0006568 - tryptophan metabolic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="TrpB-like pyridoxal phosphate-dependent enzyme"
                     /protein_id="HEX9916690.1"
                     /translation="MVSNKRQVALTTEELPKQWYNIQPDLPEPLPPPKDPEGGYSRIK
                     KLPELLIGECLKQEFSQERWIDIPEELQEIYLQAGRPRPLFRAKSLEKALDTPARLYF
                     KSEFYSPTGSHKVNTAIAQAYYAKREGYERLTTETGAGQWGTALAYGASLAGLKCTVY
                     WVRAVHDWKADRKALMQLFGAEVYASPSNRTEFGKSLYSKNPKHPGSLGIAISEGLED
                     AGKDPKAIYSLGSVLNHVLMHQTIIGLETKKQFEKFDDYPDIVISCLGGGSNFGGFAL
                     PFMHDRLKQGKKIRFIAAQSQAAPNLQGEYKYDFADYAEQTPLLKMYTLGHRTDMVPI
                     KGDGLRYHGCSPILSLLRDKGYIDTVAYPSDEKHVFNRTKVFIQTEGFLPAPESAYSV
                     ACAIDEALKCKESGEKKAIAFNISGHGFLDMPGYREVLGLK"
     gene            5691..6971
                     /locus_tag="VGB16_03050"
     CDS             5691..6971
                     /locus_tag="VGB16_03050"
                     /inference="COORDINATES: protein motif:HMM:NF013594.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="M48 family metallopeptidase"
                     /protein_id="HEX9916691.1"
                     /translation="MTKKLVLIFFSGLLLFSVLSPIVGWAQQTSQIVSDKTQLVTAVE
                     QKDTVFNQDQKQRLEQATKYQRTKHLLYFLGQGYGLVFLFLFCFLGISARIRSKAEKI
                     TKLRFFVIALFIVAFILIQFIISFPLDYYGFNLEHKFNLSNQTFGGWMGDYSKEFLLS
                     LLLFLILFEGVYFLIKKSPKRWWIYVSAVFVLFTIVLVNLAPILILPLFNVYTPLPQG
                     ELRDKLVRLSERAKVRTEAIYTMDMSKQTEKVNAMFTGLGNTKRIVLGDNLVKHLNPN
                     EVEVVIAHEMGHNILQHIWRIIVLNAILATFGFLLIHLTMGRIVNRWKKSLKMESPSD
                     IATLPLFLLIFMIYSLVIMPVMPGYSRILEQQADRFALEITHNKGAFVSSMQKLVYLN
                     LDDPNPSPVVEFLLYDHPATGKRIKFAEEYKFKE"
     gene            6979..8238
                     /locus_tag="VGB16_03055"
     CDS             6979..8238
                     /locus_tag="VGB16_03055"
                     /EC_number="4.2.1.33"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_014163692.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="aconitase/3-isopropylmalate dehydratase large
                     subunit family protein"
                     /protein_id="HEX9916692.1"
                     /translation="MPDKTIIEKILSKASGKDVSADDRVWADIDLTVIRDFGGPNAVL
                     EFDKFTGKGKVKDPDKIAITFDYQAPAKDLKVAQNQALCRQFAKRQGIKHFFDVNWGI
                     GQHVLLEQGMIKPGSVVVGTDSHMNLLGSIGSFSSGVGTTDIVAAWYCGKLWFRVPET
                     IKVIFKGKYKEPTCAKDLTLGFVKTIGPDKPNYKSLEFVGKPIDNLSLAGRISLASMV
                     TEINAKICFIQPSGETLDFLNKRCKEKIEIVAPDKNAIYLEEMEIDVDDLKPQIACPH
                     TPFNVVSVEEVEGKPFNQGFIGSCTNGRFEDFESAAKILKNKKIHPEVRLIIVPATSE
                     VALQMLKAGLYEVFMQAGAMIANPGCSLCTAGHHGVLGKGDVLLSTSNRNFLGKLGKG
                     AEVYLCSPATVAASAIEGKITDPRKFW"
BASE COUNT         2557 a         1489 c         2035 g         2213 t
ORIGIN      
        1 ttgtcagtta aaagagaaat attcttagag gcaaaaaaac tgaaatggat tcatctgggc
       61 gtagctggga ttgacaagac cctttttcct gaggttttga agagtgaagt tctagtaacc
      121 tcgtccaagg gaatacatca agtcaccgtt tccgagcatg ttatgggtct ggttttggca
      181 tttgccaaag gaattgccgc aagcataaaa gcacaaccaa caaaagtatg gctacaaaga
      241 gagcttattg ataagagatt caatttggaa ggaaaggtat tgggcattgt tgggttggga
      301 aacatcggtt tggaattagc ccgaaaagcc aaagccttcg atatgaaggt gattgccttg
      361 aagaatagtg tgaaaaaagg ggagaaatat aaaaacgttg atttgttatt aggcaaggaa
      421 aaactgaagg aattattgag ccaatccgat tttgtggtta tactgcttcc cttaaccaaa
      481 gaaacttacc atctgattag ccagaaagaa ttgggatgga tgaaaaggga ggcgtatctt
      541 ataaacgttg cccgaggtcc tataataaat gaagaagatt tgatatttgc cttaaaaaat
      601 aaaaccataa agggtgcggc actggatgtg gttgagaaag aaccactgga ctcaaacagt
      661 cccttatacg actttgagaa tgtaataatc acacctcata tagcgggaag catgccggat
      721 tattacaaaa gagttggaga gatattcaag gagaacttaa ttagatattt gaagaagagg
      781 ccacttctaa atttggtgga taagaaaaaa gggtattaaa tgagcattct ggttgacaag
      841 aatagcaaaa tcattgtcca ggggataacc ggcggagcag gaacttttca taccaaaagg
      901 atgctggaat acggcaccaa tgtagtggct gggacctctc caggtaaagg tggagaaaag
      961 gttttgggcc ttccggttta cgatacagtt gaggagtgcg taagaaatca ccgagcagat
     1021 accagcgtga tatttcttcc tgccaaattt gttatggatg ctgcaattga ggcaatttat
     1081 gctggaataa aatttatcgt ggtgattccg gagcacattc ccatccagga tatgctttta
     1141 atcagaaaag aagcagtcaa gcatagtgcc cgggtactgg gaggaaatac agccggtata
     1201 ataactccgg gagaagctaa cttaggtata atgccggata tagctttcgg taaaggaaga
     1261 gttgggactg tgtcccgctc agggtcactc acctattatg tggctgacac cctaactcgc
     1321 tcaggatacg gcgaaacaac ctgtgttggc ttgggtggag acccgatttt aggaagcacc
     1381 tttgatgaga tattatggtt gtttgaaaaa gacgaaaaaa cgaaagcagt ggttatggta
     1441 ggggagattg gtggagttta tgaagagaga acaaaggata ccatatctaa aatgacaaaa
     1501 ccggtaattg ctatgatagg cgggttatac gcaccccaag ggaaaagaat ggggcatgcc
     1561 ggtgcaattg tagaaggcca gatgggaacc gcacaaagca aattgaaagt cttggaggaa
     1621 gctggcgccc atcgggcaaa tacttttttg gagattgtgg agatattaaa gaagttggga
     1681 gtgtaaccat tgaacaatag ccgattcctt ctagtgttta ttttgttatt cttgctcttg
     1741 attggttgcg ctcataggca aaagttagcc tttattgacc ttcagaaaca aaagcgagta
     1801 gggaatctag ttgggaatgt atatataatc gagaatgctg aaatcgattc cttgcaggga
     1861 agtactttct cttatccgct taaatatgta agaaacggta agaaaacagt ggtattaatt
     1921 gaaggactgc gccgcccgaa ttttccttac atgcctgcca aagtggaccc tccgcctttc
     1981 tattgtttct tagcggaaat agagggactt aactacaacg tagggtacga tccaagggag
     2041 gaagaggttt tggggcttat gccatggttg gggggaaaac gattagaagt ggaacactgt
     2101 tttctgtatt ttagtgccgc ccaagatgcc ataatagaag gttttgttac aatcactttt
     2161 tcttatggtg aaggtgagca gcaggggcgt atgttgacgg gcaaatttaa ggggaagaga
     2221 gttgaaagaa aggatctttt taagtagcgc gtgtggggcg aagtcgagct tcgcccctac
     2281 gcgtaagaac ttattgtagt tgccacattt atggggcgat tttgatttgt tataaattcg
     2341 ctcgataaat cgagcaacta caatgaaaac cttaggaata tatgtccgag attgtagcta
     2401 aagtctatcg caacgattgt gtggagagcg tgcattgtgg gtcaattgtg gtggttgatt
     2461 ccaaggggga gataatttat caattgggtg acccatattt caaaacttat tttcgctcat
     2521 ccgctaaacc attccagatc attccggttt tgaaaagcgg ggcgatagaa aaatacaata
     2581 taactgaaaa ggaaatcgcc gttatgatta gctcgcacag cggagaggaa agacatgtca
     2641 aaaccgtatc ctctattcta aaaaagatag gtctttcaga aaagaacctc caatgcggca
     2701 cacatccgcc tttgtatcta actgagctcg gcatattgcc acgaaaggaa gataagttca
     2761 gcccacttca gcataacttt tcaggcaagc attcgggaat gctacttctc acagtgcata
     2821 aaggatggga tgtgaaaact tatttaaatc ccaatcatcc aacccaaaag ataatcctga
     2881 aaacaatagc agatatttgc gaatatccct tgaataaaat aagagtcgga acagatggtt
     2941 gtggggcacc aatcttctac attccgattt acaatatggc tcgaggattc gccaaattag
     3001 ctatttccga ggatatatat ttgaaacaag tcgtgagatg tatgtctaaa tatcctgaaa
     3061 tggtatcagg acaaaatcgt ttcgatttag tcttgtcaca aaccttggac aaaaggggag
     3121 tttgtaaagg tggggcggag ggtttacatt caactgcctt attggaaaag ggatggggag
     3181 ttgtggttaa aatcagcgat ggaaaccata gggcaatccc accagtggca ctggaagcct
     3241 tgaggcagat gggtatattg aaaagacagg agttggcaaa aatagaaaac tggacaaatc
     3301 ccaaggtaaa gaactatcaa gggaaaatag tgggctacat aaaagccgat ttcaaactga
     3361 aaaaagggga ttgaaatgag agcaaaaatc ataaaactct tgcctgagtt aagaaagata
     3421 aaggataaag agctcaagga gaaggtcgta ttagcttggg aaatgggaat ctttgaaggt
     3481 gggtataagg aaggtgattt aaagaagatt tgtttcacgt tgttgattcc agaatgcaaa
     3541 gtgagtttaa ttcagcatac ccgagcagta accagcaccg ccttagccat tgcaaaggat
     3601 ttgacaagaa actatggtag caaggtgaaa atcaattatg attatctgat cgccggtgga
     3661 cttttgcatg atgtgggcaa gcttctggaa tactgcatag ataaaaatgc cgtgtgcaaa
     3721 agcaaaaacg gaaagttctt aaggcacgca ttttccggaa cagctttggc ttacgtttgt
     3781 ggcataccgg cggaggtatt gcacataata gcggttcatt cgaaagaggg ggataccagc
     3841 ccacgttcaa tagaggcgat gatagttcac tatgcagatt acattcattt tgaagcatta
     3901 ggtggaaaac tctaaaatca taaaacgtca caaggaggag aaatggtatc caacaaaaga
     3961 caagtggctt tgacaacaga ggagttaccc aaacagtggt ataatattca acccgaccta
     4021 ccagagccac ttccaccacc caaagacccg gaaggcgggt actctcggat aaagaagtta
     4081 cctgaacttt tgataggaga atgcttgaag caagaattct cacaggagag atggatagac
     4141 attcccgagg aattacagga aatctatctt caggcaggaa ggcccagacc actttttaga
     4201 gccaaaagtt tagaaaaggc tctggacact ccggctagat tatacttcaa atccgaattc
     4261 tatagcccca ccggaagtca caaggttaat accgcaatcg ctcaagctta ctatgctaaa
     4321 agagaaggat acgagagatt gaccactgaa actggagcag ggcagtgggg aacagctctg
     4381 gcttatggtg ccagtttagc cggattaaaa tgcacagtct attgggtcag ggcagtgcac
     4441 gactggaaag cggacagaaa agccctgatg caacttttcg gagccgaagt ttacgcttcg
     4501 cccagtaaca ggaccgaatt tggcaaatcc ctgtatagta agaatccgaa acatccgggc
     4561 tctctgggaa ttgccatctc tgaaggctta gaggatgcag ggaaagaccc taaagctatc
     4621 tattcattgg gctcggtttt gaatcatgta ctgatgcatc aaaccataat cgggctggag
     4681 acaaaaaagc agtttgaaaa attcgatgat tatccggata tagttatttc ctgtttaggt
     4741 ggcgggagca atttcggtgg atttgcgtta ccatttatgc acgaccgtct gaagcaaggg
     4801 aaaaagataa ggttcattgc ggcccaaagc caagccgcac ctaacttgca gggagagtat
     4861 aaatacgatt tcgctgatta tgctgagcaa acgccacttt tgaagatgta caccttaggg
     4921 cacaggacgg atatggttcc cataaagggt gacggattga ggtatcatgg ttgttctccc
     4981 attttgagtt tattaagaga taagggatac atagatactg tggcttatcc ttcggatgag
     5041 aagcatgtat tcaatcgcac gaaggttttc attcaaactg aaggatttct gccagctccg
     5101 gagtcggctt acagtgtggc ttgtgccata gatgaagcat tgaaatgcaa ggaatccggt
     5161 gaaaagaaag ccatcgcctt caatattagc gggcacggat ttctggatat gcctggttac
     5221 cgggaagttt tagggttgaa gtgaagcctc cccgtgataa atcacggggc ttcccaaata
     5281 tgagaggccc tcggcttcgc cgagaggcga gcgccgagag gcgagcgaaa cttcgcctct
     5341 ttgaggcgag catcccccta cgggggcttt ctggcagagg cgagtaatca agttagatag
     5401 cttctttctg ttattagcat ttagtaagtg cttggtctat tatagaccag gcacttagta
     5461 tttgtgctct gattgtgctc agcttgggta aatatcagcc ccaaagtctt ctcaaatata
     5521 atatttgata tttgtttcct ggcatattat attccttctg aagttttcca taagcttgct
     5581 ataaggaagc gggtagggaa cggttgcacc gttcccaaaa agataaaaga cagtagaagt
     5641 aatggcacag ccattactta cccaattcgt taataattag aggagggtat atgacaaaga
     5701 aactggtctt gattttcttc tccggcttgt tattgttttc agtgttatca ccaattgttg
     5761 gctgggctca gcagacgtcc caaatcgtat ctgataagac acaactagtc acggcagtcg
     5821 aacaaaaaga cacggtattc aatcaagacc aaaagcagag actcgagcaa gccacaaaat
     5881 atcagaggac aaagcatctt ctttattttc ttggtcaggg atatggtttg gtctttttat
     5941 ttctcttttg ttttttggga atatctgccc ggataagaag caaagcggag aagattacta
     6001 agcttaggtt ttttgtaatt gccctattca tcgttgcttt cattctcatt caatttataa
     6061 tctcttttcc actggattat tatggtttca atttggagca taaattcaat ctctccaatc
     6121 aaacttttgg gggctggatg ggtgactact ccaaggagtt tttgttatct ctgctattgt
     6181 ttttgatttt gttcgaaggg gtctattttc tgataaagaa gtcacccaaa agatggtgga
     6241 tttatgtttc cgcggttttc gttttgttca ccattgtact ggtgaacctg gcacccattt
     6301 tgattctgcc tttatttaat gtttatactc ctctgcctca aggtgagctt agggataaat
     6361 tggtgaggtt atccgagcga gcaaaggtgc gcaccgaagc catttatact atggatatga
     6421 gcaagcaaac cgagaaagtc aatgctatgt tcaccggctt gggaaacacc aaaagaatag
     6481 tattgggaga taacctggtg aagcacctga atccgaatga ggtagaagtg gtcatcgccc
     6541 atgagatggg gcataatatt ttgcagcata tctggagaat aatcgtcttg aacgccatcc
     6601 ttgccacttt tggattttta ctaattcatc tgactatggg aaggatagtt aatcgttgga
     6661 agaaaagctt gaagatggag tcaccgagtg atattgccac cttgcctttg tttttgttaa
     6721 tctttatgat ttatagtctg gtgattatgc cggttatgcc gggatattcc agaattttgg
     6781 agcagcaagc ggacagattc gctttggaga ttactcacaa caaaggcgcg tttgtctctt
     6841 ctatgcagaa gttagtttat cttaacttag atgaccctaa tcccagcccg gtggttgaat
     6901 ttctattgta tgaccatcca gctacgggta agaggataaa gttcgctgaa gagtataagt
     6961 tcaaagagta ggagagttat gccagacaaa accatcatag aaaaaatctt gtccaaagct
     7021 tcaggcaaag acgtatccgc agacgacaga gtctgggcgg atattgattt gacggttatt
     7081 cgggatttcg gtggtccgaa tgcagttctt gaatttgata aatttaccgg taaaggaaaa
     7141 gtaaaagacc cagataaaat cgccataact ttcgattatc aagccccagc caaagattta
     7201 aaggtggcac agaatcaagc attatgcagg cagttcgcca agagacaggg gataaaacat
     7261 ttctttgatg tgaattgggg aattggacag catgtattat tggagcaggg aatgataaag
     7321 ccgggctcgg tggtggtcgg cacagacagc catatgaatt tattaggctc aattggctct
     7381 ttttcatccg gggttggaac aacggatata gtggcggctt ggtattgtgg aaagttgtgg
     7441 ttccgagttc cggaaacgat aaaagtgata ttcaaaggaa aatacaaaga gccaacttgc
     7501 gctaaggatt taacgttagg gtttgttaaa acaatcggac cagataaacc gaattataaa
     7561 tcgctggagt tcgttggaaa accgattgac aatctatctt tagctggaag aataagtttg
     7621 gcaagcatgg ttacggaaat caacgccaaa atatgcttta ttcagccgag tggagaaact
     7681 ctcgatttct taaataaaag atgtaaagag aaaatagaga tcgttgcgcc ggataaaaat
     7741 gcaatttatt tagaagaaat ggaaatagat gttgatgatt taaaaccaca aatagcctgt
     7801 ccgcatactc ctttcaatgt tgtttcggtg gaggaagtcg aaggaaaacc atttaaccag
     7861 ggattcattg gctcttgcac taacggccga tttgaggatt tcgagtccgc agccaaaata
     7921 ttgaagaata aaaaaatcca tccggaagtt agactgataa ttgttccggc aaccagtgaa
     7981 gttgccttgc agatgctcaa agcgggatta tatgaggtct ttatgcaggc aggggcaatg
     8041 attgctaatc ctggttgtag tttatgcact gcagggcatc acggagttct gggcaaaggg
     8101 gatgttctgc tttccacttc aaacagaaat ttcttaggta aattaggcaa aggagcggaa
     8161 gtttatctgt gcagcccagc cacggttgcc gcatctgcca ttgagggtaa aatcaccgac
     8221 ccaagaaagt tttggtagaa atgtagcgga gctatgaacc gcaatggttc acaccagagg
     8281 tcttatacac atca
//
LOCUS       DASWLN010000092         4612 bp    DNA     linear   ENV 20-FEB-2024
DEFINITION  MAG TPA_asm: Candidatus Zixiibacteriota bacterium isolate
            SMAG_U2949 k141_592069, whole genome shotgun sequence.
ACCESSION   DASWLN010000092 DASWLN010000000
VERSION     DASWLN010000092.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37960594
            Sequence Read Archive: SRR10376091
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Candidatus Zixiibacteriota bacterium (soil metagenome)
  ORGANISM  Candidatus Zixiibacteriota bacterium
            Bacteria.
REFERENCE   1  (bases 1 to 4612)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 4612)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (26-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/12/2024 08:28:26
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 1,440
            CDSs (total)                      :: 1,416
            Genes (coding)                    :: 1,412
            CDSs (with protein)               :: 1,412
            Genes (RNA)                       :: 24
            rRNAs                             :: 1 (16S)
            partial rRNAs                     :: 1 (16S)
            tRNAs                             :: 21
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 4
            CDSs (without protein)            :: 4
            Pseudo Genes (ambiguous residues) :: 0 of 4
            Pseudo Genes (frameshifted)       :: 0 of 4
            Pseudo Genes (incomplete)         :: 3 of 4
            Pseudo Genes (internal stop)      :: 1 of 4
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..4612
                     /organism="Candidatus Zixiibacteriota bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_592069"
                     /isolate="SMAG_U2949"
                     /isolation_source="soil"
                     /db_xref="taxon:2053527"
                     /environmental_sample
                     /country="USA: Oregon"
                     /lat_lon="42.99 N 123.63 W"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            complement(<1..258)
                     /locus_tag="VGB16_03060"
     CDS             complement(<1..258)
                     /locus_tag="VGB16_03060"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEX9916693.1"
                     /translation="MATYKEKTQTWKFGPPYPRGIYWLLEVFSIVSAIIVIVIFTKGA
                     ILLPLIPMSDYMKPAFPWGISILIVACAIMIPSFLFVFIYSP"
     gene            complement(541..1098)
                     /locus_tag="VGB16_03065"
     CDS             complement(541..1098)
                     /locus_tag="VGB16_03065"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_012583271.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="LemA family protein"
                     /protein_id="HEX9916694.1"
                     /translation="MGWISILIFLGILFILFVTLYNRLVTLRNLVKNSWHQIEVQLER
                     RADLIPNLVEAVKGYAAHEKGVFENIAKARAMFSEAIEVKQKAEATNMLTSALKSLFA
                     VVENYPELKANQNFLKLQEELSGIESRIAYERESFNDAVLQFNNTQQIFPYSMVAYLF
                     GLTPRYYFEVDEPLKRQAPEVGFGK"
     gene            1220..2638
                     /locus_tag="VGB16_03070"
     CDS             1220..2638
                     /locus_tag="VGB16_03070"
                     /inference="COORDINATES: protein motif:HMM:NF013123.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_process: GO:0006508 - proteolysis [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="DPP IV N-terminal domain-containing protein"
                     /protein_id="HEX9916695.1"
                     /translation="MIAQIKISLFRILILLVILISVLSIFYFGKREIRPKLAEPFATI
                     QRFLDRNQPDSAIAALRLYLTADSADPTLYYLIGKANFQKGELHLARNYFIRSLELNS
                     DQKEMRSFLAQIDFDLAQTYWEKDKKEALFYLISVLKNTEDKNLLEKVAQLTGGVYKI
                     EQLTNDIFRDDGPSFSPDGKKIIYHSDTSYYGEEYPLKKKITKRSKLFVLDLLENKRV
                     CLTQDSYSESFGRFSPDGNRIVFQRENSLPLDSGGTLNPEQDLILRDLKTGQEEQLTS
                     DKGYEGLASFFPDGQSIAYVKDYSIYVMNLESRKTKNVYFSGKNILTKLQVKPLLRPI
                     SPFYPNISPDGKKIVFQAGFDKRKIYLMDLRNSKITCLTYSEEDELYPSFSPNGKRVL
                     FVLENELYLMDTNGKNRIKLTNDRTEKKNPTFSPDGKTILFCAKGQNQDDHYFEIYLL
                     HLDESISKPALNDRLKRIAQSL"
     gene            2635..3711
                     /locus_tag="VGB16_03075"
     CDS             2635..3711
                     /locus_tag="VGB16_03075"
                     /inference="COORDINATES: protein motif:HMM:NF014244.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="site-2 protease family protein"
                     /protein_id="HEX9916696.1"
                     /translation="MKISRAEIEQRIYQLRQLLGDVFELDAVYHLANQTFFQGRPLFP
                     QNVLWKESIQERLAQYGYTSNIEQNGDRFILTVSKKGEEKEKKIPWTNILLFLASVVT
                     IFMAGAMIDKQDIVSNPLLIIKGASFAIPLILMLSFHEFGHYFVSRRRKIDVSLPYFI
                     PGPTIFGTFGAVIKSKSPFKNRRDLIDVGAAGPIAGFVVAIVATIIGLGQSEIVPVLT
                     KGVLTFGDSIIFRILSWMVLGNLPPEHKILVSPTAFAGWAGIFVTMLNLLPLGQLDGG
                     HILYGLLGRKQHLVAWLIIPLLFAMGWFYSPLWFMWAGLALIMRPAHPPTLNDSLQLD
                     LKRKIIGWIAIAIFVISFIPIPIR"
     gene            3735..>4612
                     /locus_tag="VGB16_03080"
     CDS             3735..>4612
                     /locus_tag="VGB16_03080"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEX9916697.1"
                     /translation="MSLTKIFLICILVVITFSFHALAIDLSRGILLDIPNEKAELQFG
                     KLPPLQLTKDELAKMASYRFGADTLKVLAILVEWSNRPGSYPKETFDSLLFSRNVFPG
                     GSVADYYYEVSYGKLNVVGDVVDWYNAGTYTSWFNFESLFPILNPVIDYSKYDGDYDW
                     NVDAVVFIRSGNGQEDSHDENDIWSYAIVYPLGSGPGPYDGVRMPRWNTSPETRPLHN
                     PNHPPDFSGVDTLNKIRVFCHELAHNVGLPDLYDYDAKLDIPTFYTPNDDNDHPIYDW
                     CVTGYGGYGILSIGSEV"
BASE COUNT         1319 a         1022 c          939 g         1332 t
ORIGIN      
        1 gggagaatat atgaaaacaa agagaaaact tggaatcatt atggcacaag ctacaatcaa
       61 aattgaaatc ccccagggaa aagctggttt catgtaatcc gacatcggaa taagcggcaa
      121 aaggatggca cctttagtga atattacaat cactattatg gcagacacaa tcgaaaacac
      181 ctcaagcaac cagtaaatcc cacgcggata cggcggccca aacttccagg tctgtgtctt
      241 ttccttgtaa gttgccatag ttatccttta tcgtctcgcg taggggtgga gtttccttcg
      301 actttgctca ggacggtgag caaggtcgaa ccgtatctcc acccgatttt tacagggcga
      361 ccaagatagg tcgccttcgt tcttagtcgt cattcctgcg gaggcaggaa tccatcccgt
      421 agttgcccaa ttcattgggc gtcttcaaaa accgcctctc tttgtggtgt aggttctcaa
      481 agctcaaacc tcgaaactaa aaccatgcac cattacggta cagggcaaaa ctgatttaac
      541 ctactttcca aacccaacct ctggtgcctg tcttttcagc ggctcatcca cttcaaaata
      601 atatctggga gtaaggccaa aaagataagc caccatgcta tacgggaata tctgttgggt
      661 attattgaat tgcagaaccg catcgttgaa cgattcgcgc tcataggcaa tccgactttc
      721 gattccagat aattcctcct gtaactttag gaaattctgg ttggctttaa gctccgggta
      781 attttccacc accgcgaaaa gggatttcag ggcggaagta agcatattgg ttgcttccgc
      841 tttttgcttg acttctatcg cttcgctgaa catcgctctg gctttggcga tattttcgaa
      901 aacccctttt tcgtgtgctg cataaccttt taccgcctcc accaaattgg gaatcaaatc
      961 cgcccttctc tccagttgta cttctatctg atgccagctg tttttgacta aattacgtag
     1021 tgtgaccaaa cgattgtaaa gcgtcacaaa caaaataaat aaaatgccaa gaaagatgag
     1081 aatagaaatc catcccatat tttctccata tttttgcgaa tttccctgct tactcgtaat
     1141 atcggactgc taaacaattg gcttaactct ctattctctt gacaaaaacc tatctctgcc
     1201 ttattttgcc ttttaaatta tgatagcaca aataaaaata tctcttttta gaatccttat
     1261 cctattggtt attcttattt ccgtcctctc cattttttac ttcggaaagc gcgaaatcag
     1321 accgaaatta gcagaaccgt ttgccactat tcaaagattt ctggaccgaa accagcccga
     1381 ttccgccata gcggctttac gcctttatct aaccgcagat agtgccgacc ccactttata
     1441 ttatcttata ggcaaagcta attttcaaaa aggggagctt catttagccc gaaactactt
     1501 tatccgctct ttggaattga actctgacca gaaggaaatg aggtccttct tagctcaaat
     1561 cgactttgat ttagcccaaa cgtattggga aaaagataaa aaggaagccc tgttttatct
     1621 tatttccgtt ctgaaaaata cggaggataa aaatttactg gagaaggtag cccaactcac
     1681 tggcggagtt tataagatag agcaactgac taatgacatc ttccgagatg atggcccatc
     1741 cttttcaccg gatggcaaaa agataatcta tcactcggac acaagttact acggcgaaga
     1801 gtatccgcta aagaaaaaaa taactaagcg aagtaagctt tttgttctgg atttacttga
     1861 gaacaaaaga gtctgcttaa ctcaagatag ctattcggaa tcatttggca gattctctcc
     1921 agatggaaac agaatagtct ttcaaaggga aaattccctc cccttggatt caggaggcac
     1981 cttaaatcct gagcaggatt tgattctaag agatttaaaa acaggtcaag aagaacaatt
     2041 gacttcggat aaaggttatg agggcttagc ttcttttttt ccggatgggc aaagcatagc
     2101 ttatgttaaa gattatagta tctatgtaat gaatttggag agtcgcaaaa ccaagaatgt
     2161 ctatttttca ggtaagaata tcctaaccaa gctccaggtt aagcctttgc tccgtcccat
     2221 ttctcctttt tatcctaata tttcaccgga cggaaagaaa atagtctttc aggcaggatt
     2281 tgacaaaaga aaaatctatc ttatggattt gaggaattca aagataactt gcctgactta
     2341 cagcgaagag gatgagcttt acccttcatt ctcaccgaat ggaaagaggg ttctattcgt
     2401 attagaaaat gagctgtatc taatggacac aaacggcaag aacagaatca agttgactaa
     2461 cgaccgaact gaaaaaaaga accccacttt ctccccggac ggcaaaacaa tcctcttttg
     2521 tgccaaagga caaaaccagg atgaccatta ttttgaaata taccttctgc atttagatga
     2581 gtccatctcc aaacccgcgc tgaatgacag attgaaaagg attgcacaaa gtttatgaaa
     2641 atctcacggg ctgaaataga acaaaggata tatcaactca gacagctcct cggggatgtt
     2701 tttgaactgg atgcggttta tcatttggca aatcaaacct tctttcaagg taggccgctc
     2761 tttccgcaaa atgttttgtg gaaagagtca attcaggagc ggctcgctca atacggatac
     2821 acttcaaata tagaacaaaa cggcgaccgc ttcattttga ctgtgagcaa gaaaggagag
     2881 gaaaaagaaa aaaagattcc ctggactaat atcctgcttt tcttagcgtc agtggtcacc
     2941 atctttatgg ctggggcgat gatcgacaag caggacatag tatccaatcc ccttctcatc
     3001 ataaaaggag cttcttttgc cattcctctt atcttgatgt taagttttca tgagttcgga
     3061 cattattttg tttccagaag aaggaaaata gatgtcagct taccctattt cattcccggt
     3121 ccaaccattt ttggtacgtt tggagcagtt ataaaatcta aatctccttt taagaacagg
     3181 agggatttga ttgatgtggg ggcagctgga ccaattgccg gatttgttgt cgctatagtg
     3241 gcaaccataa tcggcttggg acaatcagag attgtgccag ttctgactaa gggggtgttg
     3301 acctttggag attcaatcat attccgtatt ttgtcttgga tggttttggg caaccttcca
     3361 cccgaacata aaatccttgt cagtccaact gcatttgctg gctgggcagg aattttcgtg
     3421 actatgctca atcttttacc cctcggacaa ctggatggtg gacatattct ctatggactt
     3481 ttgggaagaa aacagcatct ggtcgcctgg ctgattattc ccctgttgtt tgcgatgggt
     3541 tggttttact cgcccctgtg gttcatgtgg gctggactgg ctttgattat gaggcctgct
     3601 catcctccta ctttgaatga ctcattgcag ctggatttaa aaagaaaaat catcggctgg
     3661 atagccatag ccatttttgt aatctccttc atccccatac ccataagata aaaaccttta
     3721 aaccaggaga gaatatgagc ctgacaaaaa tctttctaat ctgcatactt gttgtaatca
     3781 cgttttcctt ccatgctttg gccatcgatc tttccagagg aatactcctt gatattccaa
     3841 acgagaaagc agaattgcaa ttcgggaaat tgcctcccct ccaacttact aaggacgaac
     3901 ttgctaagat ggcttcttac cgttttggcg ccgacactct taaggtatta gccattctgg
     3961 tagaatggtc aaaccgacct ggcagctacc ctaaagagac ttttgacagt ctgcttttct
     4021 cccgcaatgt ctttcccgga ggttcagtgg ctgactacta ctatgaagtc tcttatggaa
     4081 aacttaatgt agtgggagat gtagttgatt ggtacaatgc cggaacttac actagttggt
     4141 tcaatttcga gtcgttattc ccgatactca acccggtgat agattattca aagtatgatg
     4201 gagattatga ctggaatgtg gatgcggtgg ttttcatccg ttcgggcaac ggtcaggaag
     4261 actctcacga tgaaaatgat atctggtcgt acgctatagt ctatccctta ggctccggtc
     4321 ccggacctta cgatggagtg cgcatgccca gatggaacac ctctccggag acaagaccgt
     4381 tgcacaaccc caaccaccct ccagattttt ccggcgtgga tacattaaat aaaatacgtg
     4441 tcttctgtca cgaattagct cataatgttg gcttacctga tctgtatgac tatgacgcta
     4501 aattggatat tccaactttc tacacaccca atgacgacaa cgatcatcca atctatgact
     4561 ggtgcgtcac gggttatggt ggctatggca tactttccat cggctccgag gt
//
LOCUS       DASWLN010000093         8462 bp    DNA     linear   ENV 20-FEB-2024
DEFINITION  MAG TPA_asm: Candidatus Zixiibacteriota bacterium isolate
            SMAG_U2949 k141_974635, whole genome shotgun sequence.
ACCESSION   DASWLN010000093 DASWLN010000000
VERSION     DASWLN010000093.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37960594
            Sequence Read Archive: SRR10376091
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Candidatus Zixiibacteriota bacterium (soil metagenome)
  ORGANISM  Candidatus Zixiibacteriota bacterium
            Bacteria.
REFERENCE   1  (bases 1 to 8462)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 8462)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (26-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/12/2024 08:28:26
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 1,440
            CDSs (total)                      :: 1,416
            Genes (coding)                    :: 1,412
            CDSs (with protein)               :: 1,412
            Genes (RNA)                       :: 24
            rRNAs                             :: 1 (16S)
            partial rRNAs                     :: 1 (16S)
            tRNAs                             :: 21
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 4
            CDSs (without protein)            :: 4
            Pseudo Genes (ambiguous residues) :: 0 of 4
            Pseudo Genes (frameshifted)       :: 0 of 4
            Pseudo Genes (incomplete)         :: 3 of 4
            Pseudo Genes (internal stop)      :: 1 of 4
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..8462
                     /organism="Candidatus Zixiibacteriota bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_974635"
                     /isolate="SMAG_U2949"
                     /isolation_source="soil"
                     /db_xref="taxon:2053527"
                     /environmental_sample
                     /country="USA: Oregon"
                     /lat_lon="42.99 N 123.63 W"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            <1..494
                     /locus_tag="VGB16_03085"
     CDS             <1..494
                     /locus_tag="VGB16_03085"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=3
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEX9916698.1"
                     /translation="YEYINCNLLSEVLKVANYLEVDIDDPLELLHRANQELYRLYLTV
                     EKLHREKERMQEKIIDSARKQTALDSLQTVLATFSHYINNSTTTIMGRAQLIEFAIKN
                     KEIEDRDGRVSASIKLILQSVENISTVLEEMKKLVSFDTVKYHGDSKIIAMGEGMKAT
                     SVK"
     gene            755..973
                     /locus_tag="VGB16_03090"
     CDS             755..973
                     /locus_tag="VGB16_03090"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEX9916699.1"
                     /translation="MSTQTVSPEVIPKKTNAFKSLTNWFNHSFSTYEIEPKDKTKFKL
                     IDKRDKEFVREVSKNQIARLRRRINQVS"
     gene            1065..1550
                     /locus_tag="VGB16_03095"
     CDS             1065..1550
                     /locus_tag="VGB16_03095"
                     /inference="COORDINATES: protein motif:HMM:NF014518.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0009424 - bacterial-type flagellum hook
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="flagellar cap protein FliD N-terminal
                     domain-containing protein"
                     /protein_id="HEX9916700.1"
                     /translation="MASSTIQGIISNLNTDQIVEAIIQFEHRNVDLLTNRKTEKTNEL
                     TTWQSINTLFLALKTQTSLLAKDSTWNAAKVDVSDTDVLTVSTTGTVQTGTYFFSVDA
                     LAQSHQIASQGYADTNTTEIGTGGTLVIGSGNIEHQRERDNCREAAKPICLYGGTDLC
                     G"
     gene            1525..1635
                     /locus_tag="VGB16_03100"
     CDS             1525..1635
                     /locus_tag="VGB16_03100"
                     /inference="COORDINATES: protein motif:HMM:NF014604.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEX9916701.1"
                     /translation="MVEQICAASALKDTKIVDQIIPLLDTIKEGWEKIDV"
     gene            1710..2198
                     /locus_tag="VGB16_03105"
     CDS             1710..2198
                     /locus_tag="VGB16_03105"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEX9916702.1"
                     /translation="MQERNLNIKQTEKEIILALQEELLYHQAVTDILTKQKAVVRNDS
                     LGKLDQLFGQMRKEQLCIEESRRKFKDLTETLYASGIIPCLEINKLMNQIEKVTNINL
                     TLLKETEKLTIFKRDKIKLELKSLANSRQLSKYGRTSAPLLSLWIKGTESFKAERPIA
                     DK"
     gene            2216..2686
                     /locus_tag="VGB16_03110"
     CDS             2216..2686
                     /locus_tag="VGB16_03110"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEX9916703.1"
                     /translation="MKINNLDNSLNLGGSERKKVSFRKKDPTTEPDSIEISDKAKTLQ
                     DQSGKTVKFTEVQGGGLSYLLKDKKIEQAKANLSSGLYEKQEYKEETADKLINSTELK
                     DITYDLNSLKEANQSKEKSAKIEEIRQKIASGFYNRAEVLDEIAKKLTKEFGFE"
     gene            2710..3603
                     /locus_tag="VGB16_03115"
     CDS             2710..3603
                     /locus_tag="VGB16_03115"
                     /inference="COORDINATES: protein motif:HMM:NF020252.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="HDOD domain-containing protein"
                     /protein_id="HEX9916704.1"
                     /translation="MLTDAQAKQEAKLRSIISRIHNLSTPVMVFNQINRVINSPNTSA
                     YDVAAIISEDPAISAKVLKLTNSAFYGIPRTVTNIKQSIVILGLEAVKSLVLSASVFD
                     MFARNQIDKEYQEEFWRHSLACAFASRIIARSFKTKTLLEGEVFFSAGLLHDIGKLVI
                     CGFMPQEHKTIKEIIQRENLEAHQAEDRVFGFNHAHIGNVLAENWHLPENLQNAIRFH
                     HNPQDSPVNKEQNYIIYFANYLSKLLDKTQNQWETVPPPEENSWLILGISQELLPTFL
                     TQLQDEYNRAETFIKMAKGEE"
     gene            4041..4114
                     /locus_tag="VGB16_03120"
     tRNA            4041..4114
                     /locus_tag="VGB16_03120"
                     /product="tRNA-Val"
                     /inference="COORDINATES: profile:tRNAscan-SE:2.0.12"
                     /note="Derived by automated computational analysis using
                     gene prediction method: tRNAscan-SE."
                     /anticodon=(pos:4075..4077,aa:Val,seq:gac)
     gene            4262..6178
                     /gene="thrS"
                     /locus_tag="VGB16_03125"
     CDS             4262..6178
                     /gene="thrS"
                     /locus_tag="VGB16_03125"
                     /EC_number="6.1.1.3"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_013908194.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0005737 - cytoplasm [Evidence IEA];
                     GO_function: GO:0004829 - threonine-tRNA ligase activity
                     [Evidence IEA];
                     GO_process: GO:0006435 - threonyl-tRNA aminoacylation
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="threonine--tRNA ligase"
                     /protein_id="HEX9916705.1"
                     /translation="MSKIKITLEDRTTREYTKGITPFQVSEELHSGSTSKWLVAKVNE
                     NVVDMSYPINEDATLEFLGFDSPAGKEVYWHSTSHVMAQAVRELFPEVKLGIGPAIDQ
                     GFYYDFDREISFTSEDLDKIEKRMQEIIKEDYPFKRIELSKESAIELFSKKGEKYKLE
                     LISEIPGDKVTLYQSNSFVDLCRGPHLPSTGRIKAFKLLSIAGAYWRGKERNPMLQRI
                     YGTSYPEQKQLDEHVNWMEEIKKRDHRKLGKELDLFGIYPESGAGLVVWHPKGARIRR
                     IIEEFWVDEHYQNGYDLIYSPHIARLKLWEISGHTGFYQESMYAPMKIDEEDYQIKPM
                     NCPYHILVYKSKIRSYRDLPVRWAELGTVYRYERSGTLQGLMRVRGFTQDDAHIFCRP
                     DQLEDEMEKLLDFTFYLLKSFGFSQYDIFLSTRPDKAIGHPGDWDKATETLRKSLEKK
                     NLDYQVDEGGGAFYGPKIDIKIKDLLGRPWQCTTIQFDFNLASRFDLSFIGQDGKEHR
                     PFLIHRALLGSLERFFGVMIEHYAGAFPLWLAPVQIIVMPITDGQNSYASEILNILRK
                     EKLRVELDDRNEKIGYKIREAESQKIPYMLVLGKKEQESKTLSVRKHRQGDLGSMILE
                     KLLEQLKDEIKAKT"
     gene            6220..6717
                     /gene="infC"
                     /locus_tag="VGB16_03130"
     CDS             6220..6717
                     /gene="infC"
                     /locus_tag="VGB16_03130"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_013810002.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003743 - translation initiation factor
                     activity [Evidence IEA];
                     GO_process: GO:0006413 - translational initiation
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="translation initiation factor IF-3"
                     /protein_id="HEX9916706.1"
                     /translation="MNQRIRVPQVRLIDLGGQQVGIISTREALMMAEEKGYDLVEISP
                     SAKPPVCKIMDYGKYKYEQNKKERSAKKKQHVVHLKEMHFTPKTEEHDYEFKLRHVRH
                     FLEEGCNVKIVIQFRGRELVHREFGANLMQRIVKDLEDLGAPEQVQKFEGRNMILTLL
                     PKDKT"
     gene            6811..7005
                     /gene="rpmI"
                     /locus_tag="VGB16_03135"
     CDS             6811..7005
                     /gene="rpmI"
                     /locus_tag="VGB16_03135"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_018704319.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0000315 - organellar large ribosomal
                     subunit [Evidence IEA];
                     GO_component: GO:0022625 - cytosolic large ribosomal
                     subunit [Evidence IEA];
                     GO_function: GO:0003735 - structural constituent of
                     ribosome [Evidence IEA];
                     GO_process: GO:0006412 - translation [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="50S ribosomal protein L35"
                     /protein_id="HEX9916707.1"
                     /translation="MPKIRTNRSAAKRFRRTAKGKFKRSKAYTSHILTKKRAKRKRGL
                     RKSTLVHKADEKRVRRLLPN"
     gene            7024..7374
                     /gene="rplT"
                     /locus_tag="VGB16_03140"
     CDS             7024..7374
                     /gene="rplT"
                     /locus_tag="VGB16_03140"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_013702916.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0000315 - organellar large ribosomal
                     subunit [Evidence IEA];
                     GO_component: GO:0022625 - cytosolic large ribosomal
                     subunit [Evidence IEA];
                     GO_function: GO:0003735 - structural constituent of
                     ribosome [Evidence IEA];
                     GO_process: GO:0006412 - translation [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="50S ribosomal protein L20"
                     /protein_id="HEX9916708.1"
                     /translation="MSRATHGVASRRRKKKVLKKAKGYFGGRRKLYRTARETVNRAMV
                     YSYRDRRNKKREFRRLWIARINAAVRAHGLSYSFFIRALKKVGVNLDRKNLADIAVRD
                     SATFAKLVEMAKTA"
     gene            7387..8403
                     /gene="pheS"
                     /locus_tag="VGB16_03145"
     CDS             7387..8403
                     /gene="pheS"
                     /locus_tag="VGB16_03145"
                     /EC_number="6.1.1.20"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_013908198.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0009328 - phenylalanine-tRNA ligase
                     complex [Evidence IEA];
                     GO_function: GO:0004826 - phenylalanine-tRNA ligase
                     activity [Evidence IEA];
                     GO_process: GO:0006432 - phenylalanyl-tRNA aminoacylation
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="phenylalanine--tRNA ligase subunit alpha"
                     /protein_id="HEX9916709.1"
                     /translation="MELKEKLSNIDRELVSELKKVKTTDQLEKIKVRYLSRKGKISLL
                     LRDLKNLPAEQRPQFGKLFNEKKEWLKQELAKLSQQISVTPQKGPLSFDYTLHGRKSG
                     VGKLHPITQGINEIVEIFYGMGFEVALGPDVETDYYNFEALNFPPDHPARDMQDTFYV
                     GDDVLLRTHTSPVQIRVFEKRKPPVRMLAPGRSYRCEAINARSFCIFHQVEGFYVDEN
                     VTFADLKGTLAVFIKRFFGEKVKFRFRPSFFPFTEPSVEVDISCLICKGKGCSLCKQR
                     GWLEILGAGMIDPNVFKAVGYDSDKYSGYAFGLGVERPIQLKYGIDDIRLFYENDLRF
                     LRQF"
BASE COUNT         2795 a         1579 c         2018 g         2070 t
ORIGIN      
        1 attatgaata tataaactgt aacctcttaa gtgaagtgct gaaagtagcg aattacctgg
       61 aggtggacat agatgatcct ttagagttgc tccatcgagc taatcaggaa ttatatagac
      121 tttacttaac agtggagaag cttcatcgcg aaaaggagag gatgcaggag aaaataatag
      181 attctgctcg gaagcaaact gctttggatt ctctgcaaac agtattggca accttttcgc
      241 attacatcaa taactctacg accaccataa tgggaagggc ccagctgatc gagttcgcca
      301 taaagaacaa ggagatagaa gaccgagatg gaagggtttc tgcctccata aagttgatct
      361 tgcagtcagt ggagaatatc tccacggttt tagaggagat gaagaagttg gtttctttcg
      421 acacggtcaa atatcatggg gattcgaaga taattgctat gggtgaagga atgaaggcta
      481 cttccgtaaa atgacttctc gacacattga gcggttcaag ctcaacccct tcgaggtcac
      541 aaaattaggt taagaaattt taggatatgc cgattattaa aaaagaaata atggaaagca
      601 atttcctctt gtcaaagcca aaaaccttaa aactaatgtt aggggatgat agccaggaca
      661 aaaacagatg ctcacaaacc aaggctccaa gtggagcctg aaggtcaatg acggcaaagt
      721 aactggtagc gcagacccta gagaaagcta gagaatgagc acccaaactg taagcccaga
      781 agtaataccg aaaaagacaa atgcctttaa aagcttaacc aactggttta accattcctt
      841 ttcaacctac gagatagagc caaaggataa aacgaaattc aagttaattg ataaacgaga
      901 caaagaattc gtaagagaag tttccaagaa tcaaattgcc agactaagac gcagaataaa
      961 tcaggtttca taaagtgaac ggattcactt tcaaaggaga atacaaaaag ggtcagaggg
     1021 ggaaagagta accttctgac cttttattta tagggaggca agaaatggct agcagcacaa
     1081 ttcaaggcat aatatccaac cttaataccg accagattgt ggaagcgata atacagttcg
     1141 agcatcgcaa tgttgacctc ctgaccaatc gaaaaaccga aaagacgaat gagctgacca
     1201 cctggcagag cataaacaca ctgtttttag ctctaaaaac tcaaactagt cttctggcta
     1261 aggattcaac ctggaatgct gccaaagtcg atgtttcgga tacggatgtt ttgactgttt
     1321 cgactactgg cacagttcag accggaacat acttcttctc ggtggatgcc ttggctcaaa
     1381 gccatcaaat agcaagtcaa ggttatgctg ataccaatac cacagagata ggaacgggtg
     1441 ggactttggt tattggctca ggtaacatag aacaccaaag agagagggat aattgcagag
     1501 aagctgcaaa acctatatgc ttatatggtg gaacagatct gtgcggctag tgctttaaag
     1561 gatactaaga tcgtggacca gatcattccg cttttagata ccataaagga agggtgggag
     1621 aaaatagatg tttaagataa gtcggcggca ggggctatga aaggtatcac tgcaaaggaa
     1681 acctctcaac tatcggttaa ggtctgaaaa tgcaagagag aaatcttaat atcaagcaaa
     1741 cagaaaaaga aattatactt gctttacaag aggaacttct ttatcatcaa gcagtgacgg
     1801 atatcctcac caagcaaaaa gctgtggtaa gaaacgatag tctgggaaaa ctggatcaat
     1861 tatttgggca aatgcgcaaa gagcaattat gcatcgaaga aagcaggaga aagtttaaag
     1921 acctaaccga aacgctttat gccagtggaa ttataccttg tctggagata aacaagctga
     1981 tgaatcagat cgagaaagtg acaaacataa acttaacact cttaaaggaa accgagaaac
     2041 tgactatttt caaacgagat aagattaaac tggagcttaa gagtcttgcc aattcgaggc
     2101 aactctccaa atacggaaga acgagtgcac ccctcctcag tttgtggata aaaggaactg
     2161 aaagtttcaa ggctgaaagg ccaattgccg ataaatagaa tagaggtagt ttgatatgaa
     2221 gataaataat cttgataaca gtctcaatct gggtggttca gagagaaaga aggtgagctt
     2281 ccgtaagaag gatcccacca ccgaaccgga ttcgattgag atttcggata aggccaaaac
     2341 cctgcaggac cagagcggaa aaacggttaa attcactgaa gtccagggtg ggggattgag
     2401 ttacttactt aaggacaaga aaatcgagca agctaaggcc aatctcagct cagggttata
     2461 tgaaaaacag gaatacaaag aagaaaccgc ggacaaactg ataaactcaa ctgagttgaa
     2521 ggatataacc tatgacttga attcgctcaa agaggctaac cagagtaagg agaagagcgc
     2581 gaaaatagag gagatcagac aaaaaatagc cagtggtttc tacaaccggg cagaggtttt
     2641 ggacgaaata gcgaaaaagt tgacaaagga atttgggttt gaataatcct atagtctctc
     2701 aagcaaatta tgctaactga tgcgcaagct aagcaagaag cgaagctgag gtcaataatc
     2761 agccgaatac acaacctatc cacacccgtg atggtgttta atcaaatcaa ccgggtcata
     2821 aatagtccca acacctcagc ttatgatgtt gcagccataa tctctgagga cccggccatt
     2881 tcagctaagg tcttgaagtt gaccaattcg gccttctacg ggattccccg aacagtgacc
     2941 aatatcaaac aatcgattgt gattctggga ttggaggcgg tcaaaagttt ggtcttgagt
     3001 gcatccgtgt ttgatatgtt tgcccgcaac cagatagaca aagaatacca ggaagagttc
     3061 tggaggcatt ccctggcttg cgctttcgcc tcccgaataa tcgcccgaag ctttaagacc
     3121 aaaactttac tcgaggggga ggtgttcttt tcagcaggtc ttttgcatga tatcgggaaa
     3181 ttggtgatat gtggctttat gcctcaagaa cataaaacaa taaaagaaat cattcaaagg
     3241 gagaatctgg aggctcatca agcagaagat agggtttttg ggttcaacca cgcacacata
     3301 ggaaatgtct tggcagaaaa ttggcatcta cctgagaacc tgcaaaacgc tattcgcttt
     3361 catcacaatc cccaggattc acccgtgaac aaagaacaga attacattat ctattttgcc
     3421 aattatttaa gtaagctttt ggacaaaaca caaaatcagt gggagaccgt gccacctccg
     3481 gaagagaatt cctggcttat tttaggaata agtcaagaac ttctaccaac ttttctcacc
     3541 cagctgcaag atgaatacaa ccgggcagag accttcatta aaatggctaa aggcgaagag
     3601 taataataaa aacaggagtt caattcctct tgacaataat attgtggaat tttatatttg
     3661 ctctgattag aggcgttgtt ttcagttagc tatttcgaca cagttgtgtt tttggggagt
     3721 ggtgaggact ctcgaaactt taaatcataa actcaaggca gtaagcggtt cgtggttcat
     3781 agtttatggt aaaaagtttt tactcgcctg tgccagaaag cccccgtagg gggatgctcg
     3841 cctcaaagag gcgaagtttc gcctttggcg gaaatggcga ggagagcctc gccgaagtcc
     3901 cgcagagcgg gacgaaggcg ggcttaaaag aaggcttcgg cgaagtttcg ccttttacat
     3961 tcgggaagcc ccgtgattta tcacgaggag gctttactat gaaccgtaaa ctgtgaacca
     4021 agaacttgtg tgtagcacac gggcgattag ctcagttggt tagagtgctt gcttgacatg
     4081 caagaggtca ctggttcgat cccagtatcg cccacttaaa acagtttcga gttttgagtt
     4141 ttcggtttta agaatctcga aaccatattc tttaaaattt gaactatttg gaaagctatg
     4201 atataaactg gagcaataaa gattaccctc agcggggtaa tttgttaata ggggagacgg
     4261 cttgagcaaa atcaaaataa ctcttgaaga ccgaaccaca agagaatata cgaaagggat
     4321 tactcccttt caggtctccg aggaattgca ttccggctca acttcaaagt ggttagtggc
     4381 taaagtgaac gagaatgtag ttgatatgag ctatccaata aatgaggacg ctactttgga
     4441 atttttgggt ttcgattcac cagccggaaa agaggtttac tggcacagta cctcacatgt
     4501 tatggcacag gctgttcggg agctatttcc tgaagtgaaa ctgggaatag gcccggcaat
     4561 tgaccaggga ttctattatg attttgacag agagatatca tttacttccg aagatttgga
     4621 taaaatcgaa aagaggatgc aggagataat aaaagaggat tatcccttca agagaataga
     4681 gctttctaaa gagagcgcca ttgagctttt ctccaaaaaa ggagaaaaat ataaactgga
     4741 gctgatatct gaaatcccag gggataaggt taccctctat cagagtaaca gttttgtgga
     4801 cttgtgtcgt ggaccacact tgccctcaac tggaaggata aaagctttca agctcttaag
     4861 catagctgga gcttactgga gaggcaagga gagaaatcct atgcttcagc ggatttacgg
     4921 cacatcctat cctgagcaaa agcaattaga cgaacatgtg aattggatgg aggaaataaa
     4981 gaaaagagac cacagaaaac taggcaagga attagacctt ttcggaatct atccggaatc
     5041 cggtgccgga ttggtggttt ggcatcctaa aggagctcgg attcggagaa taattgagga
     5101 gttctgggta gatgaacact atcaaaatgg atatgattta atctattctc cacatattgc
     5161 ccgtttgaag ctgtgggaga taagcgggca taccggcttc tatcaagaga gtatgtacgc
     5221 ccctatgaag atcgatgagg aagactacca gataaagccg atgaattgtc cctatcatat
     5281 tttggtctac aagtccaaaa taaggagtta ccgagacctt cctgtgcgtt gggctgaatt
     5341 aggcacagtc tatcgctacg aaaggtcagg taccttgcag ggtctgatgc gggtcagggg
     5401 atttacgcag gacgacgccc atatcttttg ccgacctgac caattagagg atgagatgga
     5461 aaagcttctg gatttcacct tttatttgct gaagagtttt ggtttcagcc aatatgatat
     5521 ttttctgtca actcgtccag ataaggcgat tggtcacccc ggggactggg acaaggcaac
     5581 cgaaactctg cgaaaatccc tggagaagaa aaacttagat taccaggtgg atgagggcgg
     5641 aggtgctttt tatggaccca aaatagatat aaaaataaaa gacctgttag gtcgaccctg
     5701 gcagtgcacg accatccaat ttgatttcaa tttggcgagc aggtttgacc tttcctttat
     5761 aggccaggac ggaaaagaac acaggccctt cttgattcat cgggcactgt tgggttcgtt
     5821 ggagcgcttc tttggggtga tgattgaaca ttatgccgga gcatttccct tgtggcttgc
     5881 cccggtgcaa ataatagtaa tgcctatcac tgatggacaa aattcctatg cctcagagat
     5941 tctcaatatt ttgaggaagg aaaagcttag agtagaattg gatgaccgaa atgagaaaat
     6001 aggttacaag ataagggaag ccgaatccca gaagattcct tatatgttag ttcttggcaa
     6061 aaaagaacaa gagagcaaga ctttatccgt aagaaaacac aggcagggtg accttgggag
     6121 tatgattttg gagaagttgc tcgaacaatt aaaagatgag ataaaagcga aaacataaaa
     6181 ttgttggagg tgataagtat aaaaccagga gaaatcagag tgaatcaaag aattcgcgtt
     6241 cctcaggtga ggttaattga tttgggcggt caacaggtgg gaatcatctc gaccagggag
     6301 gcgctaatga tggctgagga gaaaggatat gacttggttg aaatatcccc ctcagccaaa
     6361 ccgccggtct gtaagataat ggactacggc aaatacaaat atgagcagaa taaaaaggaa
     6421 agaagtgcca agaagaagca acacgtagtt cacctgaagg aaatgcattt cactccaaag
     6481 actgaagagc acgactatga gttcaagctc aggcatgtgc gacatttctt ggaagagggg
     6541 tgcaatgtta aaatagtgat acagttcagg ggtagagagt tggtgcatag ggaattcggg
     6601 gcaaacttga tgcaaaggat agtaaaagat ttggaggatt tgggtgcacc agaacaagta
     6661 cagaaattcg aaggtcgaaa tatgatattg accttactac ccaaagataa gacgtaatat
     6721 ttctattgcg cattttcacg tttttcaata tattaaaaaa acaatataaa gtttcacttt
     6781 aagtctaatg actagctaaa ggaaaagttt atgcccaaaa tcagaactaa taggtcagcc
     6841 gctaagaggt ttagaaggac tgctaaagga aagttcaaaa gaagcaaagc ttacaccagt
     6901 catattctta ctaaaaagag agctaagcga aaaaggggtt tgaggaaatc aactttagtt
     6961 cacaaagcag acgagaagag agtcagaaga ttattgccta actagaaata tcaggaggaa
     7021 cgaatgtcca gagcaaccca tggagtggct tccagaagaa gaaagaaaaa ggttctgaaa
     7081 aaagctaaag gttatttcgg gggtagaaga aaattatacc gcacagccag ggaaacagtt
     7141 aaccgagcaa tggtttactc ttatcgcgac cgcaggaaca agaaaaggga attccggcgc
     7201 ttatggatag cacgaattaa cgctgcggtg cgggcacacg gcctatctta cagcttcttc
     7261 atacgagcgt tgaaaaaagt gggggtgaat ttggaccgca aaaacttggc tgacatagct
     7321 gtgcgtgatt cagccacctt cgccaaatta gtggaaatgg ctaaaaccgc ttagtctgat
     7381 tatggaatgg aactaaagga aaagctttcc aatatagata gggaactggt tagcgagctc
     7441 aagaaagtta aaacaactga ccaattagag aaaataaaag ttcgctacct gagcaggaaa
     7501 ggtaagataa gcttacttct ccgcgacctg aagaacctgc ctgcagagca aaggcctcag
     7561 tttggtaagc tgtttaatga aaaaaaggaa tggctcaagc aggaattagc caagctgagc
     7621 caacaaatca gcgtcactcc ccaaaaagga cctctttctt ttgactatac tcttcacggc
     7681 aggaaaagtg gagtgggcaa acttcatccc ataacccaag ggataaatga gatagtagaa
     7741 atattctacg ggatgggttt tgaagtagct ttgggtccgg atgttgaaac tgactattac
     7801 aactttgagg ctttgaattt tcctccagat catcctgcca gagacatgca agacaccttc
     7861 tatgtcggtg atgatgtctt actgcgtacc cacacatcgc cggtgcagat cagagtattc
     7921 gaaaaacgaa aaccgcccgt aaggatgtta gctcccggaa ggtcttaccg ttgtgaagcc
     7981 ataaatgcac ggagtttctg cattttccac caggtcgaag gtttctatgt ggatgagaat
     8041 gtaacttttg ctgacctcaa agggacgctg gcagtcttta tcaagaggtt tttcggagaa
     8101 aaagttaaat tccgcttcag gcccagcttt ttccctttca ctgaaccatc agtggaagtt
     8161 gacatctcct gtttgatatg caaaggaaaa gggtgcagcc tttgcaagca acgtggctgg
     8221 cttgagattc ttggggcagg aatgatagac ccgaatgtat ttaaagcagt gggatatgat
     8281 tcggataaat attccggcta tgcttttggc ttgggagttg aaaggccaat tcaattgaaa
     8341 tatggcatcg acgatattcg cctattttat gaaaatgatt taagattctt aaggcagttc
     8401 taattatgag aattagctac aattggctaa aagaattagt ccatttcgat tggtcgccgg
     8461 ag
//
LOCUS       DASWLN010000094         2550 bp    DNA     linear   ENV 20-FEB-2024
DEFINITION  MAG TPA_asm: Candidatus Zixiibacteriota bacterium isolate
            SMAG_U2949 k141_338967, whole genome shotgun sequence.
ACCESSION   DASWLN010000094 DASWLN010000000
VERSION     DASWLN010000094.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37960594
            Sequence Read Archive: SRR10376091
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Candidatus Zixiibacteriota bacterium (soil metagenome)
  ORGANISM  Candidatus Zixiibacteriota bacterium
            Bacteria.
REFERENCE   1  (bases 1 to 2550)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 2550)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (26-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/12/2024 08:28:26
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 1,440
            CDSs (total)                      :: 1,416
            Genes (coding)                    :: 1,412
            CDSs (with protein)               :: 1,412
            Genes (RNA)                       :: 24
            rRNAs                             :: 1 (16S)
            partial rRNAs                     :: 1 (16S)
            tRNAs                             :: 21
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 4
            CDSs (without protein)            :: 4
            Pseudo Genes (ambiguous residues) :: 0 of 4
            Pseudo Genes (frameshifted)       :: 0 of 4
            Pseudo Genes (incomplete)         :: 3 of 4
            Pseudo Genes (internal stop)      :: 1 of 4
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2550
                     /organism="Candidatus Zixiibacteriota bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_338967"
                     /isolate="SMAG_U2949"
                     /isolation_source="soil"
                     /db_xref="taxon:2053527"
                     /environmental_sample
                     /country="USA: Oregon"
                     /lat_lon="42.99 N 123.63 W"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            complement(277..732)
                     /locus_tag="VGB16_03150"
     CDS             complement(277..732)
                     /locus_tag="VGB16_03150"
                     /inference="COORDINATES: protein motif:HMM:TIGR00072.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hydrogenase maturation protease"
                     /protein_id="HEX9916710.1"
                     /translation="MKKKILIAGVGNLLFTDEGVGVHIIRGLSKKELPEEVELAEIGT
                     ATFELIRLVDGKDKVIIIDAIISDETPGTIYRLTPEELKLDKKKLITSLHQFGVLEAL
                     ESAFQLGNKPEVIIFGVTPKDYQTLGMELTPELKKSMDRIVEAILKEIK"
     gene            complement(965..2521)
                     /locus_tag="VGB16_03155"
     CDS             complement(965..2521)
                     /locus_tag="VGB16_03155"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_013174572.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="nickel-dependent hydrogenase large subunit"
                     /protein_id="HEX9916711.1"
                     /translation="MTKKIVIDPVTRIEGHLKIEVEVENGKVTDAKTSGEMFRGWEVI
                     LKGRDPRDAQMITQRICGVCPSPHAQASTLCLDDAFGIHPPDNGRIIRNLLLASNLIQ
                     SHILHFYHLCALDFVDITAILKYTGSDPGLNKLKAWAKDEVDFQRPNAVAPFLPRYKG
                     DYIEDTETNIQAIAHYVKALDMTKKAHEMLCIWGGRMPHEIAIMPGGVCEKPTVDKIT
                     SFLYRLKELQSFIDNVYVPDVIAVAKAYPKYFEIGKGCGNLLSYGVYEENVEGTKKYF
                     SAGTYIDGELASFDPSKIAEYVKYSRFSSGTGLHPSKGETTPQPDKKKAYSWVKAPRY
                     EGKPMEVGPLARMVIEHLKGENPVLSKMVNDTLGMFNAPVNALFSVLGRHATRALECK
                     LVADKCAEWVMQLDPSKPVHTKFEIPDASTGMGLTEGPRGALGHWITIKNKKIDMYQC
                     VVPTTWNAGPRDDKNQPGPMEQALIGTPVADPENPIEPARVVRSFDPCLACAIHIIEP
                     QKDIRKFRVV"
BASE COUNT          622 a          617 c          552 g          759 t
ORIGIN      
        1 gtagctctgg ctattgcggt atttttcaat aagaggagtg gattagaaaa atagaattgg
       61 atgaatagta tatattgcga aaaaggaagt tttctaaaag attgatttca ctcgcctccg
      121 ccagaaatga cgaggcgagc ttcggtcgcc tttggcgaac ctcgcttaag gcgggcgaag
      181 ttttgctcgc ctctcggcgc tcgcctctcg gcgaagccga gggaagccga gggcctttta
      241 tattttggaa actccgtgat ttatcacggg gaggcttcac tttatttcct tcaggatagc
      301 ttcaacaatt ctatccattg atttcttgag ctccggggta agttccatac cgagtgtttg
      361 gtaatctttg ggggttacgc caaagataat cacttccggc ttatttccca gctgaaaagc
      421 ggattccaaa gcttccagaa ctccaaattg atgtagtgag gttatgagtt tctttttgtc
      481 tagtttcagt tcctctgggg ttagtctata gatagttccc ggtgtttcat ctgatattat
      541 ggcatctatg attatcacct tgtcttttcc atccaccaat cttatcaact caaaagtggc
      601 tgttccgatt tccgccaatt ccacttcctc gggcaactcc tttttggata gtcctcttat
      661 gatatggacg cccacaccct catccgtgaa gaggaggttt ccgactccag cgattaggat
      721 tttctttttc atctcagtta gtgaacctcc ccctaagggg gcatccaaaa tatctcctcc
      781 ccccttgaga ggggagaact caaggaaagg gtgagaatca tatttgctat ccccctctcc
      841 cccaagggag agagaactta tagaaatgcc tttcatcccc ctacgggcgt attctgacat
      901 gtcttacata aaaaataggg gacccatcga agcagatccc ctatttcaaa aaacacctat
      961 tcgcttaaac gaccctgaat tttcttatat ccttttgcgg ctcgattatg tgaatggcac
     1021 acgccaagca ggggtcaaag gacctcacca ctctggcggg ctcaattgga ttctccgggt
     1081 cagctacggg agttccaatc aaagcttgct ccatgggtcc tggttgattc ttgtcatctc
     1141 tcggaccggc attccaggtg gtgggaacaa cacactgata catatcaatc ttcttatttt
     1201 ttattgttat ccagtgcccc aaagcacctc tcggtccttc ggttagaccc attcccgtac
     1261 ttgcatctgg aatctcaaat ttggtatgta ccggtttgga tgggtcgagt tgcatgaccc
     1321 actcggcaca tttgtccgct accagcttgc attccaaagc tcgagtggcg tgccttccca
     1381 aaaccgagaa tagagcatta accggcgcat tgaacatacc cagagtatca ttgaccatct
     1441 tggaaagaac gggattttct cctttgaggt gctctattac cattcttgcc agaggaccca
     1501 cctccatcgg cttgccttca tagcgtggag cttttaccca agagtaggct ttctttttgt
     1561 cgggctgggg agttgtttct cccttagaag gatgaagccc agtgccggag gagaatcggg
     1621 agtattttac gtattcggct atcttggaag ggtcaaaact agccaattct ccgtcgatat
     1681 atgttccggc tgaaaaatat ttcttggtcc cttccacatt ctcctcatat actccgtaag
     1741 acaacagatt cccgcaaccc tttccgattt caaaatactt aggatatgcc tttgccactg
     1801 caatcacatc cgggacatag acattgtcga tgaaggattg aagttctttc aatctataaa
     1861 gaaaagaagt gattttatcc acagttggct tctcacatac tccacccggc attatggcta
     1921 tctcgtgcgg catccttccg ccccatatac acagcatctc gtgggctttc ttggtcatat
     1981 ctaaagcttt aacataatgg gctatggctt ggatgttggt ttcagtatct tcgatataat
     2041 ctcctttgta tctcggtaaa aacggagcca ctgcattggg tctttgaaaa tctacctcat
     2101 ctttggccca agctttgagc ttatttaaac ctgggtcgct tccagtgtat tttaagatgg
     2161 cggtgatatc cacgaaatcc aaagcgcata aatgataaaa atggaggata tgagattgta
     2221 tgagattgga ggccaaaagc aggtttcgaa ttatccttcc attgtcagga ggatgtatac
     2281 cgaaagcatc gtccaggcag agagtagaag cttgagcatg aggagaggga caaactccgc
     2341 atatgcgttg ggtaatcatc tgggcatctc ttgggtctct gcctttaaga atgacctccc
     2401 atcccctgaa catctcacct gaggtcttgg catcagtaac ttttccgttc tccacctcta
     2461 cctctatttt cagatgccct tcgattctgg tgacagggtc aattacaatc tttttcgtca
     2521 taggttacct tcctttccta agtcaagttg
//
LOCUS       DASWLN010000095         5018 bp    DNA     linear   ENV 20-FEB-2024
DEFINITION  MAG TPA_asm: Candidatus Zixiibacteriota bacterium isolate
            SMAG_U2949 k141_468585, whole genome shotgun sequence.
ACCESSION   DASWLN010000095 DASWLN010000000
VERSION     DASWLN010000095.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37960594
            Sequence Read Archive: SRR10376091
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Candidatus Zixiibacteriota bacterium (soil metagenome)
  ORGANISM  Candidatus Zixiibacteriota bacterium
            Bacteria.
REFERENCE   1  (bases 1 to 5018)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 5018)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (26-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/12/2024 08:28:26
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 1,440
            CDSs (total)                      :: 1,416
            Genes (coding)                    :: 1,412
            CDSs (with protein)               :: 1,412
            Genes (RNA)                       :: 24
            rRNAs                             :: 1 (16S)
            partial rRNAs                     :: 1 (16S)
            tRNAs                             :: 21
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 4
            CDSs (without protein)            :: 4
            Pseudo Genes (ambiguous residues) :: 0 of 4
            Pseudo Genes (frameshifted)       :: 0 of 4
            Pseudo Genes (incomplete)         :: 3 of 4
            Pseudo Genes (internal stop)      :: 1 of 4
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..5018
                     /organism="Candidatus Zixiibacteriota bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_468585"
                     /isolate="SMAG_U2949"
                     /isolation_source="soil"
                     /db_xref="taxon:2053527"
                     /environmental_sample
                     /country="USA: Oregon"
                     /lat_lon="42.99 N 123.63 W"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            <1..1028
                     /locus_tag="VGB16_03160"
     CDS             <1..1028
                     /locus_tag="VGB16_03160"
                     /inference="COORDINATES: protein motif:HMM:NF024824.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=3
                     /transl_table=11
                     /product="tetratricopeptide repeat protein"
                     /protein_id="HEX9916712.1"
                     /translation="KRSWLNEAEKAGLKALALDPDLAEAHRALSRLYWEEGKTREAIQ
                     EAEKAVKTNPNYGEAWRLLGQYYVDIGQYPRAETALARALEVKSTEANLFGSFIDLYS
                     LSGNKKKAEKYFNKALEVQLPNMWTYGSMSDCYLWWGNLEEAKRMAYEALNIDPHSTW
                     TLGNLVQIFMVSGEGDSSSFYLNKARREMPESDWFVEMAYLALMRGNKKQAEIYSDSC
                     IQFNRFLVKEFEGLPYEYQSRLRIGLAYALEGESGKALEQAESVRNSLGESLLSVEWA
                     YGRDIVLPLSFVYSLTGQKEDAVRMLDFLVKINYYTPVYIKLHPWFKNLAGYPAFEEL
                     IKGKTVK"
     gene            1294..4221
                     /locus_tag="VGB16_03165"
     CDS             1294..4221
                     /locus_tag="VGB16_03165"
                     /inference="COORDINATES: protein motif:HMM:NF012298.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="FlgO family outer membrane protein"
                     /protein_id="HEX9916713.1"
                     /translation="MIGKTISHYRILEKLGEGGMGVVYKAQDTKLDRLVALKFLPQHL
                     TSDLTEKERFVHEAKSASSLSHPNITTIHEIDEFEGQMFIVMEYCEGKTLKEVIEKEA
                     LSIKKVLGIAIQICEGLAMAHKKDIVHRDIKSDNIMLTKEGQVKITDFGLAKLKGATK
                     LTQTRSTLGTAAYMSPEQASGEEVDQRSDIFSFGVVLYELLTKQLPFKGEHQAAIVYS
                     IINEEPQPVARYNNQVSSKLEDMVFKALAKEKEERYQHIDDLLADLRREKKSLDYVKT
                     GQIPKEAIAPKPKRKLLPFLVPASIVFILVLLFLILKPFKFEIAPEKGAVAKENSLAV
                     MYFENLVDREDKERLGEVVTNLLITGLSESQYLNVVSSQRLYDILKLLGKEGTKTIDK
                     NVASQVASKAGAKWMLLGSILQVEPQVVLTSQLVDVESGKVLASQRITGEPEEKIFSL
                     VDRLTLQLKQDLSLPSRAQKEETRPVAEVTTHSQEAYRYYLEGLDYGYKFYFAETEKS
                     LKKALELDSTFAMAYYMLANLKTGEEQRELTAKAVKYSNKVSQKERYYIESWEEYVSR
                     NYSEANKILQKAVERYPEEKEAFYYIGVTYGDKLHDSKEAIYYFNKAIQIDPLYKVAY
                     NALAYGYNEIGDFDKSIWAINKYIELAPDEPNPYDSRADLYAWNGKLDQAIESYKKAL
                     EIKPDFYMSLPKLGNMYLFKKDYARAESCFEALSSSNEKLYRSMGRLYLVLIPLYQGR
                     FEDALKVLDDGIAADRMEQVEGGMNAYKHFGKGQVYEEQKKMTLALKETEIGIQIFKK
                     AMPNNPDNGSAYYAYLLAKSGKIAEADESARALKKDIEEKNPTLMSSYWCTLGEIQLA
                     RGDTNMALGYLEKAYKESLTPSFSLRSRLAEIYLKTGRLDEAVAQLEKALSRYDDDRV
                     WYTMAVKAYYLLGLAYEKSGWNKKAIEKYEEFLDIWKNADPGIPEVADAKERVKRLKS
                     KNL"
     gene            4570..>5018
                     /locus_tag="VGB16_03170"
     CDS             4570..>5018
                     /locus_tag="VGB16_03170"
                     /inference="COORDINATES: protein motif:HMM:NF024583.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="GAF domain-containing protein"
                     /protein_id="HEX9916714.1"
                     /translation="MNKNLEQENLELKRAVEELSVLNEISAAIGSSMKVEEITKLIIS
                     KCIKKIGVEQGAVTLLKDDSTDPFKTFVRVIDRSMENVPYRLGVNLAGWMFKNQKPLL
                     INDLSTDERFTGVSPETGKIKSILSVPLKVKNKLIGLLSLFNKKGDD"
BASE COUNT         1581 a          954 c         1203 g         1280 t
ORIGIN      
        1 acaaaagatc ttggttgaat gaggctgaaa aagctggact taaagccttg gctcttgacc
       61 cggatttagc cgaggctcat cgtgctcttt ccagactata ctgggaagag gggaaaaccc
      121 gagaagccat acaagaggca gaaaaagcgg tgaaaacaaa tcccaactat ggagaagcct
      181 ggaggcttct cggacagtat tatgttgata ttggtcaata tccaagagct gagaccgctt
      241 tggccagagc gttagaggtg aaatcgactg aggccaattt atttgggtct ttcatagatt
      301 tatattcact atccggaaat aaaaagaagg cagagaaata ttttaataaa gcactggagg
      361 ttcagcttcc caatatgtgg acctatggtt ctatgagtga ttgttactta tggtggggca
      421 atttggagga agcaaaaaga atggcttatg aagcgttgaa tatagatccg cactcgactt
      481 ggacactggg caacttggtg caaatcttta tggtgtctgg agagggggac agctcttctt
      541 tttacctaaa taaagctcgt cgtgaaatgc cggaatcgga ttggtttgtg gaaatggctt
      601 acctggcact tatgagagga aataaaaagc aggcggaaat atattcagat tcctgcattc
      661 agttcaatcg atttttagtc aaagaatttg agggtcttcc ctatgaatat caaagtcgtt
      721 taagaatagg cttagcctat gcactagaag gagaatccgg aaaagctctg gaacaggcgg
      781 agagcgtgag gaatagtttg ggagaatccc tgttgagcgt ggaatgggca tacgggcggg
      841 atattgttct acctcttagt ttcgtttatt cgctcaccgg acaaaaagaa gacgcagtgc
      901 gtatgctgga ttttttggtg aagattaatt attatacccc tgtatacata aaattgcacc
      961 cttggtttaa gaatttagct ggctatccag catttgagga attgattaaa ggaaaaaccg
     1021 tcaaataatg gtagccgccc atttgggcgt aacacaacgt agccgcaatc tttccttcga
     1081 cttcactcag gatggtgagc gattcggttg agcccatcat ccctgccttg tcgaaccata
     1141 gattgcgtta cgcagacata aagtctgcgc ctaccaaaaa gaaaggccaa ctatgattgg
     1201 aaaaataata tctcattatc gaatcttaga aaagctgggt gaaggcggga tgcctgtccc
     1261 gctgaagcga agcgaaagcg ggaggtgtaa tctatgattg gaaagactat ttctcattat
     1321 cgaatcttag agaaattggg cgagggaggt atgggcgtgg tttataaagc ccaagacact
     1381 aagcttgacc gtcttgtcgc tttgaagttt ctgcctcaac atctgacctc tgatctgacc
     1441 gagaaggaaa ggtttgtcca cgaagccaaa tctgcttcat ccttaagcca tcccaatatc
     1501 accaccattc atgagatcga tgagtttgaa gggcagatgt ttatcgtgat ggagtattgt
     1561 gagggaaaga ccctgaagga agtgattgag aaggaagcgc tctccataaa gaaggttctg
     1621 gggatagcca tacagatatg tgaaggttta gctatggccc acaagaagga tattgttcat
     1681 agggacataa aatcagacaa cataatgctc accaaagaag gtcaagtcaa gataacagat
     1741 tttggtctgg caaagctcaa gggagctacc aagctaaccc agactcgctc aactttggga
     1801 acagctgcct atatgtcacc agaacaagct tccggtgagg aagttgacca aagatcggat
     1861 atcttctcct ttggagttgt tttgtatgag cttctaacca aacagctccc cttcaaggga
     1921 gaacatcaag ctgccatagt ttattccatc attaacgaag agccacaacc agtagccaga
     1981 tacaacaacc aggtttcaag taagttagag gatatggttt tcaaggcatt ggctaaagaa
     2041 aaagaggaaa gataccagca tatagatgac cttcttgcag atttgagaag agagaaaaaa
     2101 agcttagact atgtgaagac tggtcagatt ccaaaagaag cgattgcacc caagcccaag
     2161 agaaagcttc tccccttctt agtccccgcc tcaattgttt ttatcctggt tctacttttc
     2221 ctgatcctca aacccttcaa atttgaaata gcaccggaaa aaggagcagt ggctaaagag
     2281 aactctttgg ctgtaatgta ttttgagaat ttggtggacc gggaggacaa agaaagactg
     2341 ggcgaagtgg taactaatct cttgataacc ggtctttccg aatcgcaata tctgaacgta
     2401 gtttccagcc aaagactcta cgatattctg aagttattgg gcaaggaagg aaccaaaacc
     2461 atagacaaaa acgttgcttc acaggtggcg tctaaagccg gtgctaaatg gatgcttctg
     2521 ggaagcattc ttcaggtcga gccgcaggtt gttctcactt cccagttggt ggatgttgaa
     2581 agcggaaaag tattagcttc gcagcgcatc accggagagc ccgaagaaaa gatattctct
     2641 ttagtggaca gattaacact tcaactcaag caggatttat cccttccctc tcgggcacag
     2701 aaggaagaaa ctcggccggt agccgaggta actacccatt cacaagaggc ataccgttat
     2761 tacttagagg gtcttgatta tggatacaag ttttacttcg cagagactga gaaaagtctt
     2821 aagaaagcct tagagcttga ttccactttt gcgatggcat attatatgtt agctaatctc
     2881 aaaacaggag aggagcagag ggaattaaca gccaaagcag tcaaatactc aaataaggtt
     2941 agccagaagg agagatatta tatagaaagc tgggaggaat atgtatcaag gaattacagt
     3001 gaggcgaata agatactcca gaaggcagtt gaacgctatc ctgaagagaa agaggcattt
     3061 tattatatag gggttactta tggtgataag ctccacgact ccaaagaagc aatttattac
     3121 tttaacaaag ccatccagat agaccctctt tataaggttg catataatgc tcttgcctat
     3181 ggttacaatg aaatcggtga ttttgataaa tccatctggg ctataaataa gtatatagag
     3241 ttagctccgg atgaacccaa tccttatgac agccgggctg atttgtatgc ctggaatggc
     3301 aaacttgacc aggcgataga atcctacaaa aaagctttag agataaaacc ggatttttat
     3361 atgtctctgc ccaaactggg gaatatgtat cttttcaaaa aggattatgc tagagcagaa
     3421 agctgttttg aggcactttc ttccagcaat gagaaacttt acaggtcaat gggaagattg
     3481 tatttggttc tgattcctct ataccagggg agattcgaag atgcgctcaa ggtcttggac
     3541 gacggtattg ccgcagacag aatggaacaa gttgagggag ggatgaatgc atataaacat
     3601 ttcgggaagg gccaggtcta tgaagagcag aaaaaaatga ctttagcgtt aaaggaaaca
     3661 gaaataggta tacagatttt caagaaagct atgcccaata acccggataa tgggagtgct
     3721 tattacgcct atcttcttgc taagagtgga aaaatcgccg aagctgatga atcggcaaga
     3781 gctttgaaaa aggacattga agaaaagaat ccaaccttga tgtcttctta ctggtgcact
     3841 ttgggcgaaa tccagttggc aagaggggac acaaacatgg cactgggcta tttagaaaaa
     3901 gcatataaag agtccctaac cccgtctttt tccctgcgtt ctcgtcttgc ggaaatttac
     3961 ttaaagacag gcagactgga cgaggcagtg gcccaattag aaaaggcact ttcaagatat
     4021 gatgatgaca gagtatggta taccatggca gtgaaagctt actatcttct aggcttggct
     4081 tatgagaaat ccggctggaa caaaaaggcg attgaaaaat atgaggagtt tctggatatc
     4141 tggaaaaatg ctgacccggg gataccagaa gttgcggatg cgaaagagag ggtgaagaga
     4201 ttaaaaagca agaatctgta gcggtcgcat ttatgcgaca ggcttttagt tcgggaaatc
     4261 caattcccga gctaacaaga aaatcagaga gtagccgcaa tctttccttc gacttcactc
     4321 aggatggtga gcgattcggc tgagcctatc atccctgcct tgtcgaacca tagattgcga
     4381 attgacgcag acataaagtc tgcggctacc ctatagttaa cgagcacgcg aacgaaaggg
     4441 tgaagagatt aaaagaaaag atgtagatag tcaaaccgta ttattacgat tatttcttta
     4501 ctttaaatcc ccacttttta gcttgccgaa gtttgttttt cccttatatt tgaatcaacc
     4561 tgagggacaa tgaacaaaaa tcttgaacaa gagaaccttg aactaaaaag agcagtggaa
     4621 gaactttctg ttttaaatga gatatcggct gcaatcggtt ccagcatgaa agtggaagag
     4681 attaccaaac tgattatatc caaatgcatc aaaaagatcg gggttgaaca gggtgcggtg
     4741 actctcctga aagatgattc aactgacccc tttaagactt ttgtccgggt gatagacagg
     4801 agcatggaga atgttccata taggttgggc gtgaacttgg cgggatggat gttcaaaaac
     4861 caaaagcccc tcctgataaa tgacctctct actgacgaga gattcaccgg cgtgagccca
     4921 gaaacgggaa aaattaaatc tatcctctct gtgccgctta aagtgaaaaa caaactcatt
     4981 ggccttttgt ctttgttcaa taaaaagggt gacgactt
//
LOCUS       DASWLN010000096         1874 bp    DNA     linear   ENV 20-FEB-2024
DEFINITION  MAG TPA_asm: Candidatus Zixiibacteriota bacterium isolate
            SMAG_U2949 k141_800456, whole genome shotgun sequence.
ACCESSION   DASWLN010000096 DASWLN010000000
VERSION     DASWLN010000096.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37960594
            Sequence Read Archive: SRR10376091
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Candidatus Zixiibacteriota bacterium (soil metagenome)
  ORGANISM  Candidatus Zixiibacteriota bacterium
            Bacteria.
REFERENCE   1  (bases 1 to 1874)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 1874)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (26-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/12/2024 08:28:26
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 1,440
            CDSs (total)                      :: 1,416
            Genes (coding)                    :: 1,412
            CDSs (with protein)               :: 1,412
            Genes (RNA)                       :: 24
            rRNAs                             :: 1 (16S)
            partial rRNAs                     :: 1 (16S)
            tRNAs                             :: 21
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 4
            CDSs (without protein)            :: 4
            Pseudo Genes (ambiguous residues) :: 0 of 4
            Pseudo Genes (frameshifted)       :: 0 of 4
            Pseudo Genes (incomplete)         :: 3 of 4
            Pseudo Genes (internal stop)      :: 1 of 4
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..1874
                     /organism="Candidatus Zixiibacteriota bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_800456"
                     /isolate="SMAG_U2949"
                     /isolation_source="soil"
                     /db_xref="taxon:2053527"
                     /environmental_sample
                     /country="USA: Oregon"
                     /lat_lon="42.99 N 123.63 W"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            complement(<1..529)
                     /locus_tag="VGB16_03175"
     CDS             complement(<1..529)
                     /locus_tag="VGB16_03175"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEX9916715.1"
                     /translation="MNQIRETISNYAADPSGEYCRLIVRQKLFWGKSDKEEFKDLYTP
                     RNLQAALSYTTAEQNIAAALCTSLYIPDEGFNLFDATGYEKVRIVLPNLRSSKEYSST
                     RTSPIVSKLNEIWLISAAGFRGFVDINRVHRNFFYNISSSDQVEDYLQFSFQGTGGIR
                     QTSISRIPPKVLPFCT"
     gene            complement(526..1482)
                     /locus_tag="VGB16_03180"
     CDS             complement(526..1482)
                     /locus_tag="VGB16_03180"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEX9916716.1"
                     /translation="MFSASKMLPRFPRRNLPRPKVKGGTGIWGISEQAAGLGGMGYNA
                     RNNEIKSRRVCIGLLGQNHVKFSLALPGRICDNDSAKEGLMDKLNFLIQNIQSALFLE
                     SFAFRNKLAIASKINDAVGQLFDGDPMMLDLFPEAPPEIPRIQLKDSRSIYSLNFSVN
                     RIDFFYNEPEKPTKTFDSIKDEYLTNFLKINRLVKEDYHLETPRIALVIKAISAIDMG
                     SNLFLQQEFLGSKEFFKNTYALEIHALEKAALGQFPINRWFRIKTARDRLGKDGTLFV
                     EIDINTQPEKEKGLGKQEIEEFYSSAIEFAKRSFLRCFGSSL"
     gene            complement(1591..>1874)
                     /locus_tag="VGB16_03185"
     CDS             complement(1591..>1874)
                     /locus_tag="VGB16_03185"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=3
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEX9916717.1"
                     /translation="QSKLGFAFGLFNQTPVIPSHIFVYDKDNVLCYVDTPEKLCLVPT
                     DMGSNYHLNHEEMRQLLRDTLRVERGSILEPIRAIEVRPFFENRNKASL"
BASE COUNT          511 a          429 c          393 g          541 t
ORIGIN      
        1 ttgtgcaaaa tgggagcacc ttgggtggga ttcttgaaat tgaggtttga cgaatcccac
       61 ccgtcccttg gaaactgaat tgaagataat cctcaacttg gtccgaactg cttatgttgt
      121 aaaagaaatt ccgatgcacg cggttaatat caacaaaacc acgaaaccct gcggcactta
      181 ttaaccaaat ctcattcaat ttactgacaa taggacttgt tcgagttgaa gaatattctt
      241 tggatgaacg aaggttgggc aaaacaatgc ggaccttttc gtacccagtg gcatcgaaaa
      301 ggttgaatcc ctcatccgga atataaaggc tggtgcaaag tgcagcagcg atattttgtt
      361 cagccgtggt atagctcaag gccgcctgaa ggtttcttgg cgtatacagg tctttaaatt
      421 cttccttatc ggactttccc caaaatagtt tttgcctcac tataagcctg cagtattcgc
      481 cactcggatc agcagcataa ttgctaatgg tctcacggat ctggttcata aagaactccc
      541 aaaacacctt aaaaaggaac gttttgcgaa ttcaattgca ctggaataaa attcctcaat
      601 ctcttgtttc cctagtcctt tctctttttc tggttgagta ttgatatcga tttctacaaa
      661 aagcgtacca tctttcccca gacgatctct ggccgtcttt attctgaacc agcgattaat
      721 tggaaactgc ccaagggctg ccttttccaa ggcatgaatt tccagagcat acgtattttt
      781 aaaaaattcc ttactcccaa ggaattcttg ctggaggaaa aggttggatc ccatatctat
      841 tgccgatatc gccttgatga ccaatgctat tcgtggggtt tcgagatgat aatcctcttt
      901 cactaaccgg ttaattttta aaaaattagt caggtattca tctttaatcg aatcaaatgt
      961 ttttgttggt ttctcaggct cattgtaaaa gaagtcaatc cgattaactg aaaaattgag
     1021 gctgtaaatc gatctgctgt ctttaagctg tatccgagga atttcgggag gtgcctcagg
     1081 aaaaaggtct aacatcatag gatctccatc aaagagctga ccgacggcgt cattgatttt
     1141 ggaggcgata gctaacttat tacgaaaggc gaagctctca agaaaaagtg cagattgaat
     1201 attttggatt aggaagttca atttatccat cagcccctcc tttgcagaat cattatcaca
     1261 aatccggcca ggcagtgcaa gcgagaattt gacatgattt tgccccagca aaccaatgca
     1321 aactcgacgg gatttgatct cgttgtttct tgcattgtat cccatgcctc ccaaaccagc
     1381 tgcctgttcc gatattcccc aaattcccgt tcctcctttt actttgggac gcggaaggtt
     1441 tcgccgggga aatcggggca acatctttga ggccgagaac atagcggagc tcatcccact
     1501 taaagccgct atatcaggcg tgtaggcttg ctccgaattc cccaggctca gctaatgtta
     1561 aagcaatttc tgcccgaatc tcccgacctt ctacaaggaa gccttgtttc tattttcaaa
     1621 aaacggcctt acttcaatgg cgcgaatggg ctcgagaata ctgccacgtt ccacccgcaa
     1681 tgtgtcacgc aataactgtc gcatctcttc atgattcaaa tggtaattgc tgcccatgtc
     1741 ggtggggacc aggcataatt tttcgggggt gtccacatag catagaacgt tatccttatc
     1801 atatacaaaa atgtggctgg gaatcaccgg tgtctggttg aatagcccaa aagcgaatcc
     1861 gagtttgctc tgcg
//
LOCUS       DASWLN010000097        17902 bp    DNA     linear   ENV 20-FEB-2024
DEFINITION  MAG TPA_asm: Candidatus Zixiibacteriota bacterium isolate
            SMAG_U2949 k141_1055659, whole genome shotgun sequence.
ACCESSION   DASWLN010000097 DASWLN010000000
VERSION     DASWLN010000097.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37960594
            Sequence Read Archive: SRR10376091
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Candidatus Zixiibacteriota bacterium (soil metagenome)
  ORGANISM  Candidatus Zixiibacteriota bacterium
            Bacteria.
REFERENCE   1  (bases 1 to 17902)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 17902)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (26-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/12/2024 08:28:26
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 1,440
            CDSs (total)                      :: 1,416
            Genes (coding)                    :: 1,412
            CDSs (with protein)               :: 1,412
            Genes (RNA)                       :: 24
            rRNAs                             :: 1 (16S)
            partial rRNAs                     :: 1 (16S)
            tRNAs                             :: 21
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 4
            CDSs (without protein)            :: 4
            Pseudo Genes (ambiguous residues) :: 0 of 4
            Pseudo Genes (frameshifted)       :: 0 of 4
            Pseudo Genes (incomplete)         :: 3 of 4
            Pseudo Genes (internal stop)      :: 1 of 4
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..17902
                     /organism="Candidatus Zixiibacteriota bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_1055659"
                     /isolate="SMAG_U2949"
                     /isolation_source="soil"
                     /db_xref="taxon:2053527"
                     /environmental_sample
                     /country="USA: Oregon"
                     /lat_lon="42.99 N 123.63 W"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            complement(173..583)
                     /gene="nusB"
                     /locus_tag="VGB16_03190"
     CDS             complement(173..583)
                     /gene="nusB"
                     /locus_tag="VGB16_03190"
                     /inference="COORDINATES: protein motif:HMM:TIGR01951.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_process: GO:0006355 - regulation of DNA-templated
                     transcription [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="transcription antitermination factor NusB"
                     /protein_id="HEX9916718.1"
                     /translation="MTTRREARKAVLQALYAYEMRESQAEQLVEEFIQENKLDEKSAL
                     FVRELCLKVIENMERIDNLVRGCVEHWDFSRLAVVDKNILRLAIGEFLFFSDIPRKVS
                     IDEAIELAKEFSSQDSGSFVNGVLDSIAKKLNRD"
     gene            complement(1008..1472)
                     /gene="ribE"
                     /locus_tag="VGB16_03195"
     CDS             complement(1008..1472)
                     /gene="ribE"
                     /locus_tag="VGB16_03195"
                     /EC_number="2.5.1.78"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_015925454.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0009349 - riboflavin synthase complex
                     [Evidence IEA];
                     GO_function: GO:0000906 - 6,7-dimethyl-8-ribityllumazine
                     synthase activity [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="6,7-dimethyl-8-ribityllumazine synthase"
                     /protein_id="HEX9916719.1"
                     /translation="MSKTIKGILDAKGRSFAIVVSRFNEFITAKLLDGALDCLARHSA
                     DERRITIIWVPGSFEIPYAASRLAKSKKFDAVICLGTVIRGDTPHFDYIASEVTKGIA
                     RISLDTNVPVIYGLITTDNLEQAIERAGTKAGNKGWDAALSAIEMVNLFDKI"
     gene            complement(1486..3711)
                     /locus_tag="VGB16_03200"
     CDS             complement(1486..3711)
                     /locus_tag="VGB16_03200"
                     /inference="COORDINATES: protein motif:HMM:NF025910.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="tetratricopeptide repeat protein"
                     /protein_id="HEX9916720.1"
                     /translation="MKRKRTDSFLIAPDQSAVWFTRVTVFLCGAVLLFRLVSSFFPKE
                     RLWGINHLAYFSPYFAISITILGLLVLVPRINRLLQDNLRRPLNFVHELTIGKHKYLY
                     FIIFSFLSIIVFWFLRVKTYFLGDEYHLITALPAGELVFKWSEPLEIAAHLYLYELLR
                     QFMAVDGAIVYSLISYIAGVVFVFLVFLTADLLGKSKSEKVFVFSILVSMGSILLFFG
                     YAENYSLTYTAILAYLYFALRYLKNESSILPTTLMFVLGCSLHLLTFSLLPSLLFLYL
                     LKSDTKTREFVISKKRVLLYSLLSLFIITGIYVYIVKYGKLSSLLTVTVPLTAAWYSV
                     PGYTLFSFSHLLDMFNEQILVSPIGLVLLLVILIVFGLKRTLLSDRTSQFLVIVTIFQ
                     MTFHFLIDPDLGATRDWDLFSFTALGYTLLGIYLFSKLVQDKQKLRYIGAVLISTVLF
                     STLPWVMLNASTEKSLKRIIDAVDLDPKRNPQTYINLGTYLVERGLLEDAKKMGEKLK
                     VQFPWVWLTLKGQVMTAMGDLQGAEAAFKEAIQSAPMMSSAHLNLGITYFERGKMDMA
                     ILEFERAIQLNPEGIIAFKSYAWIGDVYAQRGQFDKAIQAYNKAINGEIVNKGEVYQK
                     LGRIYVQRREFDKAIEMYKESIEREINNKELVYQSLGHCYFIKNELDKAISMYQKALK
                     LDPDVIEPHAYLGHAYLKKGMKDKAIEEYKIYLKYGKDDEQIKTIKALILKLNQEKSN
                     S"
     gene            complement(3719..4927)
                     /locus_tag="VGB16_03205"
     CDS             complement(3719..4927)
                     /locus_tag="VGB16_03205"
                     /EC_number="3.5.4.25"
                     /EC_number="4.1.99.12"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_015050493.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003935 - GTP cyclohydrolase II activity
                     [Evidence IEA];
                     GO_function: GO:0008686 -
                     3,4-dihydroxy-2-butanone-4-phosphate synthase activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="bifunctional
                     3,4-dihydroxy-2-butanone-4-phosphate synthase/GTP
                     cyclohydrolase II"
                     /protein_id="HEX9916721.1"
                     /translation="MRKLDEIEEAIEDIKAGKIIIVVDDAQRENEGDMVMAASEVTPQ
                     AINFLTKNARGLICVSLTGQRLEELRIHPMVQVNTAKLGTRFTVSVDAISNTTTGISA
                     QDRAETIKALIDPKTKPEDLARPGHIFPIQALDGGVLARAGHTEAAVDLTRLAGLYLA
                     GVLCEIMDDDGTMARMPRLLKIAKDFNLKIITVKDLIEYRRKKEKLIKRIVTVDFPTK
                     FGDFKLYLYGSSIDEHHHLALVKGEVKGKNNVLVRVHSQCLTGDVFGSERCDCGEQLS
                     HALRMIEKESSGVFLYMRQEGRGIGLANKILAYKLQDSGKDTVEANEELGFKPDLRDY
                     GIGAQILVDLGLSTIRLITNNPKKIIGIEGYGLKVVERIPIEISPTPNNIKYLETKRD
                     KLGHLLNIKD"
     gene            complement(4937..5575)
                     /locus_tag="VGB16_03210"
     CDS             complement(4937..5575)
                     /locus_tag="VGB16_03210"
                     /EC_number="2.5.1.9"
                     /inference="COORDINATES: protein motif:HMM:TIGR00187.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="riboflavin synthase"
                     /protein_id="HEX9916722.1"
                     /translation="MFSGIVEETGQIKNLSRRGSSLAITVKASRVLEDLKIGDSVNID
                     GACQTTVKVNKNEFAVEAVEETLNKTTFRNMKIGQSVNLERAVKASDRLGGHILTGHI
                     DCRGKIDSIKDNDGSHIFEISLPQSNLKYLIEKGSIAVDGISLTIVEVGPKSFTVSVI
                     PHTIKNTNFAFKKIGDEINIELDIIGKYIEKMLNSKSPKSKITEEWLKKLGW"
     gene            complement(5575..6654)
                     /gene="ribD"
                     /locus_tag="VGB16_03215"
     CDS             complement(5575..6654)
                     /gene="ribD"
                     /locus_tag="VGB16_03215"
                     /EC_number="1.1.1.193"
                     /EC_number="3.5.4.26"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_012994201.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008703 -
                     5-amino-6-(5-phosphoribosylamino)uracil reductase activity
                     [Evidence IEA];
                     GO_function: GO:0008835 -
                     diaminohydroxyphosphoribosylaminopyrimidine deaminase
                     activity [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="bifunctional
                     diaminohydroxyphosphoribosylaminopyrimidine
                     deaminase/5-amino-6-(5-phosphoribosylamino)uracil
                     reductase RibD"
                     /protein_id="HEX9916723.1"
                     /translation="MDEKYMEMVLRLAKKGVGRTSPNPLVGAVIVKNGKIVGTGYHQK
                     IGSPHAETNALKSAGKKGKGATLYVNLEPCSHFGRTPPCVDSIIRNGIREVICATIDP
                     NPKVNGAGVKQLRQAGIKIKLGVLEDEARKLNEIYFKRITSGFPFVALKIVQTLDGKI
                     ATLDGDSKWISQKDSRRFVHSLRKQMDAVLVGVNTVIKDDPKLTTREVKGKNPLRIVL
                     DSEGRIPLNSNLLKKNKDNKTVIVVTTNKAGGKLKNKAQVWKVKSNYQNKIDLKVFLK
                     LAAQKGISSILVEGGREIFSSFLKEKLADKIYCFISPKIIGEGISSFGSLNIKKIKDA
                     LKLKDVELKEFSNDILLIGYLDWRR"
     gene            complement(6956..7513)
                     /locus_tag="VGB16_03220"
     CDS             complement(6956..7513)
                     /locus_tag="VGB16_03220"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_018477599.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003677 - DNA binding [Evidence IEA];
                     GO_function: GO:0004803 - transposase activity [Evidence
                     IEA];
                     GO_process: GO:0006313 - DNA transposition [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="transposase"
                     /protein_id="HEX9916724.1"
                     /translation="MRNGKPNRLKGYDYSQNGMYFVTVCIHNRIEWFGNIKNGKMVLN
                     ECGEIVEKQWAWLGDQYPYVVLDEQVVMPNHFHGMISIVGNGRMDGCVVGDGRDRSLQ
                     KIKPLPELIGAFKTTSSKLIHGTGALQFHWQKSFYDHVIRSAKSLESIRQYIANNPMK
                     WDMDIENIRNFGIDNVAYYSSIIKA"
     gene            complement(7755..8594)
                     /locus_tag="VGB16_03225"
     CDS             complement(7755..8594)
                     /locus_tag="VGB16_03225"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_013707057.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="4Fe-4S binding protein"
                     /protein_id="HEX9916725.1"
                     /translation="MCEFCTKHGEGKKWYLQAKNYGLDLASDTRRKKMISGFFGTAGE
                     SAEESLNQMKKLKTAPRIIQSLISGSITRKYKKEHFGQVVPIEDVEKIFEIINSIVRL
                     PCICRKTLTGKEERFCFGISALPAWARKEDKIDSGYLLGPYAQGLEKMEKSDALKLIK
                     EFETKGSVHSIWTFITPFIGGLCNCDRSDCLAMISTLNYDIKFMFKAEYVARVDWEKC
                     SGCRSCMQMCQFGAMGYSVARERVFIDVNHCYGCGVCRAACPNDAIVLVDRVGVKEVR
                     KNW"
     gene            complement(8615..9088)
                     /locus_tag="VGB16_03230"
     CDS             complement(8615..9088)
                     /locus_tag="VGB16_03230"
                     /inference="COORDINATES: protein motif:HMM:NF014629.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="23S rRNA
                     (pseudouridine(1915)-N(3))-methyltransferase RlmH"
                     /protein_id="HEX9916726.1"
                     /translation="MVKIRFIFLGRQKEKWIIEGVNHYKKLLGGMVDLDVKIIQPEKV
                     TERSSVLNILDKEKNKIIKSLKDKTLLIVLDNKGNELESEDLARFFKNKINQGQSDFT
                     FVVGSSLGLSEDIKKLSNFKLSLSRMTFAHQLTWIILLEQIYRAFSIIKGTKYHK"
     gene            complement(9098..9499)
                     /locus_tag="VGB16_03235"
     CDS             complement(9098..9499)
                     /locus_tag="VGB16_03235"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_013278829.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0005737 - cytoplasm [Evidence IEA];
                     GO_function: GO:0003824 - catalytic activity [Evidence
                     IEA];
                     GO_process: GO:0008150 - biological_process [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="secondary thiamine-phosphate synthase enzyme
                     YjbQ"
                     /protein_id="HEX9916727.1"
                     /translation="MIKEIPIRTSSRVELVDITSKVEEVISASKVESGISIIFVPHTT
                     AAVTINENADPSVQRDIIYELNKLVPFNDNYSHTEGNSAAHIKSSIIGASRSIIIQDG
                     SLKLGTWQGIYFCEFDGPRSRKVWITIIKTK"
     gene            complement(9492..10409)
                     /gene="ftsY"
                     /locus_tag="VGB16_03240"
     CDS             complement(9492..10409)
                     /gene="ftsY"
                     /locus_tag="VGB16_03240"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_013411935.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003924 - GTPase activity [Evidence IEA];
                     GO_function: GO:0005047 - signal recognition particle
                     binding [Evidence IEA];
                     GO_process: GO:0009306 - protein secretion [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="signal recognition particle-docking protein
                     FtsY"
                     /protein_id="HEX9916728.1"
                     /translation="MQNSHKNLKTGLERTKKSLFLKIKEVLGLHKGISDEVLEKIGEI
                     LIKADVGPEVTSQITSQLERKAKQENIRSEEDVIDTLKKQMIAVFDTISYREESKVKS
                     PWIIMMVGVNGTGKTTTIAKLAQRYKKEHKKVLLAGCDTFRAAAIEQLAIWADRVGVD
                     MVKAVPGGDPAAVAFDATTATLARKADVLIVDTAGRLHTKSSLMEELKKIKKVMGKVL
                     PGSPQEILLVLDATVGQNALSQVKLFDEAVGLSGIVLAKLDGTAKGGIVIAIADKFKI
                     PIKYVGIGEKMEDLEEFKPQDFVEAIFSD"
     gene            complement(10427..13999)
                     /gene="smc"
                     /locus_tag="VGB16_03245"
     CDS             complement(10427..13999)
                     /gene="smc"
                     /locus_tag="VGB16_03245"
                     /inference="COORDINATES: protein motif:HMM:TIGR02168.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_process: GO:0007059 - chromosome segregation [Evidence
                     IEA];
                     GO_process: GO:0030261 - chromosome condensation [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="chromosome segregation protein SMC"
                     /protein_id="HEX9916729.1"
                     /translation="MYLSKLELLGFKSFPDKTELAFDNGVTAIVGPNGCGKTNLLDAV
                     RWVLGEQRVTLLRGSKMEEIIFNGTKELKPLGMAEVSLVVKNHKGILPIEYSEVRVTR
                     RLYRSGESEYLLNGAPCRLKDIIDLFLDTGMGTHAYSVIQPAMIEAVLSDKAEDRRLL
                     FEEASGITKYKLRKKATIRKLEITEGDILRVNDIITEVERQVSSLKRQMRRAERFKKF
                     SEEATQLEIKLAQADFGDLKAENDRLEQDLLELKKRTREHTTDLSKQEAEVERLRLEL
                     LETEKELVTYQKKLASDVEKAHQVERDVLVREERRASLEKSITQLKQELSDLENRLKL
                     FENETSAKQEELAVTTQKLKEKERELSQKENELSIVDEKRDKVRAILEDLEKQEASKE
                     EETSERKSKVANLEGSFKQLTQRRENLGQEEDVLSDRLNEIKSQINALSSACEQKRIA
                     LEGENAEKNKLEQEIKINEEELERISKQEVKIGNELSSEKARLTLFQEIIKEYQGFQD
                     GVKYILDERSSLPGVIDSVANLINTDEKYISAIESALGERAQFLICGDEASALRAIRF
                     LEEHKEGRASFLILEKVKKVTEQNKTVSIKEHEGVMGWAFNLVRCEERYKPDVMFLLA
                     DVLVVDSFERAQKMASTLKENLTLVSLSGKVIRPEGVLEGGSRKEVHLLGREKQVQGI
                     AEKVSILGNEFKGIQQEKQKKEDFLGSLNQSLSERISHISRLKEELSQKDMELKQKEF
                     ESRELTQRVEMNIKELNKLQGEAGELERSIKQMKLELEALERDNSELSKEVFETKEEL
                     DRIEEERNLQFEKTNSFRIDLVSLSGKKEQLENDLLRLNEMIDDIRKNINMRIEQTEN
                     SRKGIQEITLNNSNVDEELKGLFEQKETDEKIYNSWQGKYSQLQQEVVSRESQLKSIR
                     QIREEFQSKVHNLELGKLEKATRMRSIRDKAWEEHQVNLEDLEPLSLEERGGLENYRQ
                     RIKSLRELVSRLGPVNLVALEEYKVSKERLDFLKNQIKDLVEAKNSLNLTIGKIDETA
                     REMFLETFQKVRANFQSVFAQLFEGGEADVTLEPDIDVLESPIEISARPRGKRLVNIN
                     QLSGGEKALTATSLIFAIYLVKPSPFCILDEIDAPLDDSNVKRFLKMIRHFSQNTQFL
                     VITHNKLTMESADTLYGVTMEQPGVSKIVSVKLGRQEVLAGDQA"
     gene            complement(14002..14583)
                     /locus_tag="VGB16_03250"
     CDS             complement(14002..14583)
                     /locus_tag="VGB16_03250"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEX9916730.1"
                     /translation="MEKKIRTNFLVKNLVVWWKRRFGASSSFSFHPSFSTAKSVLVCF
                     PASKEEFASAANHLPALADIFSSSKIYLLLPLLEADSFIANCKSYEIISPRPADLKPF
                     SLPSRKFIQKIKEHDFGIAIDLDLKGCLFNVLTCFSSGTPLRIGMEGKWGKPFYNIRL
                     VTQKGLVYLDQRYDAMLETLRFLRNGTGAQRRS"
     gene            complement(14592..15320)
                     /locus_tag="VGB16_03255"
     CDS             complement(14592..15320)
                     /locus_tag="VGB16_03255"
                     /inference="COORDINATES: protein motif:HMM:NF024252.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="RodZ domain-containing protein"
                     /protein_id="HEX9916731.1"
                     /translation="MGSLGSYLKKHRESLGYTVKEVADQTRIRVHYLEAIEEDNLNAL
                     PSEPYAKGFVKAYAHFLGLESEELLNEYGWDKKKEELEQQSRPQTVESETGWAFIAAG
                     LVLGIVIVAIFVQYAQQENINYGDYTTQAIEKLALVPETAVTESLMQAEVVLPEQLVL
                     EIKPVKRTWILVIADNDTVLSGEVRPGTKLEVEAKERFVINLGKISAVEVFLNGSKLE
                     AFSQPQRRIFKAEINRQNYNQYLQ"
     gene            complement(15324..15929)
                     /locus_tag="VGB16_03260"
     CDS             complement(15324..15929)
                     /locus_tag="VGB16_03260"
                     /inference="COORDINATES: protein motif:HMM:TIGR00172.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="nucleoside triphosphate pyrophosphatase"
                     /protein_id="HEX9916732.1"
                     /translation="MTLRAEANLAGFIGKRKFTLASRSPRRMELLKEANIDFEVKTPK
                     INEENVSKNPKQHVLRLSRMKAESVLPEVEGGLVLGADTIVVLNGEILGKPKNQKEAK
                     RMLEKLSNREHKVYTGLTLIDKDKKKTVSDYECTEVKFNSLSSEGIDSYISTGEPTDK
                     AGAYGIQGRGSRLVREVKGSLDNVIGLPMGKLEEMLIHMLR"
     gene            complement(15926..16366)
                     /gene="dtd"
                     /locus_tag="VGB16_03265"
     CDS             complement(15926..16366)
                     /gene="dtd"
                     /locus_tag="VGB16_03265"
                     /EC_number="3.1.1.96"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_007202324.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004045 - aminoacyl-tRNA hydrolase
                     activity [Evidence IEA];
                     GO_process: GO:0008152 - metabolic process [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="D-aminoacyl-tRNA deacylase"
                     /protein_id="HEX9916733.1"
                     /translation="MRTVIQRVNRARVSVEGKTIAQINRGLLILVGATQGDNEEDVKY
                     LADKCTNLRIFEDQEGKMNLSVLDVGAELLVVSQFTLYADVKKGRRPSFTRALEPDLA
                     QKLYVKFIQMLRQNRLKVEQGEFGAKMAIELINWGPVTLILDSK"
     gene            complement(16700..>17902)
                     /locus_tag="VGB16_03270"
     CDS             complement(16700..>17902)
                     /locus_tag="VGB16_03270"
                     /inference="COORDINATES: protein motif:HMM:TIGR00757.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004540 - RNA nuclease activity [Evidence
                     IEA];
                     GO_process: GO:0006401 - RNA catabolic process [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="Rne/Rng family ribonuclease"
                     /protein_id="HEX9916734.1"
                     /translation="EILVQIIKEPMGTKGPRVTSELSIPGRYSVLVPEEEGVRVSRKI
                     SNRAEKKRLKGLASQLKPKDCCLIVRTVAEGKTERELRSDVKNLLRLWEKIKKEAQRK
                     KAPYLLHKEMGITSSIMRDLLTPEITSVVVDSKREYHNILSYLRSIAPNLRSKVKLYQ
                     GKVPLFDFYKIESEIDKMLDRKVWIKKGSYIVIDQTEAMVTIDVNTGRYVGKSDQEAT
                     ILKVNLEAAKEIARQIRLRDIGGLIVIDFIDMDSRENRKKLYEEFKKSMRSDRSKSSI
                     LPVDEFGLIEMTREKIRPSLMYAFSEPCPTCRGVGRILSVETLATKVERWFRRAKLAS
                     SVGRYQIVASPQLYVLFSREREDRIKNLRRELKLEIEIIEDRNLPVDKYRILSPDENL
                     EVTGLYQA"
BASE COUNT         4713 a         4420 c         3079 g         5690 t
ORIGIN      
        1 aagatgtaac tggtggcgat gaactcgccg caatcccaga agctgactgt gggagccata
       61 gtcataaaat agaccacgaa gcttactaaa aagaccaata aacccagata gatattaagc
      121 ttgtccttaa gaaaaaaaga ggaaagcaag ggagacctcc aactaaaaga atttaatctc
      181 gattaagttt ttttgctatg gaatctaaaa ccccatttac aaaactgcct gaatcctgcg
      241 agctgaactc cttagccaat tcaatcgctt cgtcgatgga gacttttctg ggaatatcac
      301 tgaagaataa aaactctcca atggctaaac gcaatatatt tttatccacc actgccaatc
      361 tggaaaaatc ccagtgctcc acgcaccctc ttaccagatt atcgatacgt tccatatttt
      421 ctataacctt caagcaaagc tctcttacga aaagggctga cttttcatcc agtttgttct
      481 cctggatgaa ctcctcaacc aactgctccg cctggctctc ccgcatctca taagcataaa
      541 gagcttgcaa aaccgccttg cgtgcttctc ttctcgtggt catatataat acccgataac
      601 ttaagtgaag cttcccccta cgggggtttc ccaaatataa gaggcgaaac ttcgccgaag
      661 ccttctttta agcccgcctt cgtcccgctc tgcgggactt cggcgaggct ctcctcgcca
      721 ttcatccccc tacgggggct ttctggcaga ggcgagtaaa agtttaattc aaaaatcaaa
      781 gtgtaaaaat caaaattaca tatcaaaatg caaaatcatt ttttccttaa attcaaattt
      841 tgcattgtca ttttgatatt tgatggttcg acttcgctcc cttcgacttc gctcagggac
      901 agtgagcaag gtcgaactgt caccatcaac cctgagccta atcgaaggat tgatttttga
      961 tatttgaatt ttcaatgccg cctctatatt acgaagccat atataattta tatcttgtca
     1021 aataaattaa ccatctctat agcggagagc gctgcatccc aacccttatt cccggcctta
     1081 gtaccagccc gctcaattgc ctgctctaga ttatccgtag ttatcaagcc ataaatcact
     1141 ggaacatttg tgtccaaaga tattcgtgca atacctttgg tcacttcgct ggcaatataa
     1201 tcaaaatgag gcgtatcgcc tcttatcacc gtgcctaaac agatgactgc atcaaacttc
     1261 ttgctcttag ctaatcttga ggcggcatac ggtatttcaa atgagcccgg aacccaaatg
     1321 atagttatcc ttctctcgtc tgccgaatgg cgggcaaggc aatccagcgc accatccaag
     1381 agtttagcag taatgaattc gttaaaacga ctaaccacaa tggcgaaact tctgcccttg
     1441 gcatccagaa tcccttttat ggttttagac ataaaccctc cttagttaag aatttgattt
     1501 ttcttgatta agctttagta ttagtgcctt gatcgtcttg atttgctcat catcttttcc
     1561 gtattttaag taaattttat actcctctat ggctttatcc ttcatccctt ttttgagata
     1621 ggcatgaccc agataagcat ggggttctat aacatcagga tctaatttaa gtgctttttg
     1681 atacatagaa atagctttat ccaattcatt cttgataaaa tagcaatgcc ctaaactctg
     1741 atacacaagt tccttgttat ttatttctct ttcaatagat tctttgtaca tctctatcgc
     1801 tttatcgaat tctcttcttt gaacgtaaat cctccccagc ttctgataca cctctccctt
     1861 attgactatc tctccattga tagccttatt atatgcctgt attgctttgt caaattgccc
     1921 cctttgagca taaacatccc ctatccaagc ataagactta aaagcaatta tcccttcagg
     1981 atttaattga atcgcccttt caaactccaa aatagccata tccatcttcc ccctctcgaa
     2041 ataagtgatt cccaaattaa gatgagctga ggacatcata ggagcacttt gaatagcctc
     2101 cttgaatgca gcctctgctc cctgaagatc gcccattgct gtcattacct gaccttttag
     2161 agtcaaccaa acccagggaa actgaacctt aagcttttca cccattttct tagcatcctc
     2221 taataatcct ctttctacaa gataagttcc taaattgatg tacgtttgtg ggttcctttt
     2281 tggatccaaa tccacagcat caatgattct cttaagagat ttctcagtgc tagcattaag
     2341 catgacccaa ggcaaagttg agaaaagtac agtggaaatc aagaccgctc caatatatct
     2401 taatttctgt ttatcttgaa ctaacttcga aaataagtaa attcccagca aagtatatcc
     2461 taatgcggta aaagaaaaca ggtcccaatc tcttgttgct cccaagtcgg ggtcgataag
     2521 aaaatgaaag gtcatctgaa atatcgtaac tattactagg aattgagaag ttctgtcact
     2581 aagtagtgtt ctctttaacc caaaaactat caaaattact aaaagaagaa ctaaaccaat
     2641 tggggaaacc aggatttgtt cgttaaacat atctaataga tgagagaagg agaaaagagt
     2701 ataacctggc acactgtacc aggctgcagt taaggggacg gtaactgtta acaaagagct
     2761 gagtttaccg tattttacaa tatatacata tattccagtg attataaaca gactaagcag
     2821 cgagtaaaga agtaccctct ttttagagat aacgaactct cttgtcttgg tatccgattt
     2881 taataaatat aagaagagga gcgaaggaag gagagagaat gttagcagat gtagtgaaca
     2941 acccagaaca aacattagag ttgttggcaa tatactgctt tcgtttttta agtatctcaa
     3001 cgcaaaataa agataagcca ggattgcggt ataggtcaaa gaataattct cggcatatcc
     3061 aaaaaatagc aaaatactcc ccatgctgac caaaatggag aacacgaaga ctttttcaga
     3121 cttgcttttc cctaataaat ctgcagtcaa gaagacaaga aagacgaaaa ccaccccagc
     3181 tatatagctt attagagaat agacgattgc tccgtccact gccataaact gtctcagaag
     3241 ttcatataaa taaagatgtg ctgctatttc caatggttca ctccatttga atactaactc
     3301 tccggctggc aaggcggtaa tcaggtggta ctcatctccc agaaaatagg ttttgactct
     3361 taaaaaccaa aatacaatta tggataaaaa actaaagatt ataaaataga gatatttgtg
     3421 ttttcctata gtcagttcgt ggacaaaatt cagtggcctt cttaagttat cttgaagcaa
     3481 gcgatttatt cttggaacca agaccaagag acccagaatc gtaatagaga ttgcgaaata
     3541 gggcgaaaaa tatgctaagt gatttattcc ccacaatctt tcttttggaa aaaaggagct
     3601 gactaaacga aatagtaaga cagcaccgca taagaaaact gtaactctgg tgaaccacac
     3661 tgcactctgg tcaggagcaa tcaaaaaaga atctgttctc ttgcgtttca taaaaaagtc
     3721 agtcctttat atttaacaaa tgccctaact tatctctctt ggtttccaag tatttaatat
     3781 tgttaggagt tggtgaaatt tcaattggaa tcctttccac aaccttcaat ccatatcctt
     3841 ctattccgat tatctttttg ggattgttgg tgatgagtcg aatggtggag agccccaaat
     3901 ccactaagat ttgagcgcct attccataat ctctcaaatc cggcttgaag cccaactcct
     3961 catttgcttc cactgtatct ttgccggaat cttgaagttt ataagccagg attttattgg
     4021 ctaagccaat tccccttccc tcctgtcgca tataaagaaa aacgcctgag ctctcttttt
     4081 ctatcattct tagggcatgg gataattgct ctccgcaatc gcaacgttct gacccgaaaa
     4141 catcaccggt taagcattga gaatgaactc tgaccagaac attattcttt cctttaactt
     4201 cccccttaac caaagccaaa tggtgatgct cgtcaattga gcttccgtaa agatacaatt
     4261 taaagtcgcc aaatttggtg ggaaagtcaa ccgtaactat tctcttgatt aatttctctt
     4321 tcttgcgacg atattcgatt aagtctttca cggtgattat ctttagattg aaatccttgg
     4381 ctatcttaag aagtctcggc attctagcca ttgtcccatc atcatccata atctcacaaa
     4441 gcacaccagc tagatacaaa ccagccaatc gagttaaatc aactgctgcc tcagtgtgtc
     4501 cagctcgggc taagacccct ccatccagag cttgaatggg aaagatgtgg cctggtcggg
     4561 ctaaatcttc aggtttggtt ttgggatcaa tcagggcttt gatggtttca gctctatcct
     4621 gagctgaaat tccagtggtg gtattggata tggcatctac ggaaaccgta aaacgagtac
     4681 ccagcttagc ggtattgacc tgaaccatag ggtgaatcct gagctcctcc aatctttgtc
     4741 cggtaaggga aacacatatc aggccgcgag catttttagt caaaaaattt atcgcctgtg
     4801 gagtgacctc actagctgcc ataaccatat ccccctcatt ttccctttgg gcatcatcca
     4861 ctacaatgat tatctttcct gctttaatat cctcaatcgc ttcttcaatt tcatcaagct
     4921 ttctcatatt ctattttcac catcctaact tctttaacca ttcttcagtt atcttacttt
     4981 tgggtgactt tgaattcagc atcttctcaa tatattttcc gataatatct aattctatgt
     5041 ttatctcatc tccaattttc ttaaaagcga agtttgtatt cttaattgta tgtggaatca
     5101 ccgaaacggt gaagcttttt ggcccaacct ccacaattgt caaacttata ccatccactg
     5161 cgattgagcc tttttcaatt aaatatttga gattggattg gggaagcgat atttcaaaaa
     5221 tatgacttcc atcattatcc tttatggaat ctattttccc tctgcaatct atatggccag
     5281 ttaagatatg gccccctagt cggtccgacg ccttgaccgc tctttctaag ttgaccgatt
     5341 ggccgatttt catatttcta aaagtggttt tgtttaatgt ttcttcgacc gcctcaactg
     5401 cgaactcatt cttgttgact ttgactgttg tctgacaagc tccgtcaatg tttacactat
     5461 ccccgatttt caaatcctcc aaaaccctgg aggccttcac ggttatggca agcgaactac
     5521 ctcgcctgga gagattcttg atttgtcccg tttcctctac gattccgcta aacattatct
     5581 tctccaatcc aggtatccaa taagcaaaat atcatttgag aattctttta attcaacatc
     5641 ttttaatttc aaagcatctt ttatcttctt gatattcaag ctgccgaaac tggatattcc
     5701 ttctccaatg atttttggag aaataaaaca gtatatctta tcagccaatt tctctttcag
     5761 gaaagagctg aatatctccc ttcctccctc taccaagata ctacttatcc ctttttgtgc
     5821 ggctaatttc aggaaaacct taagatcgat tttgttttga taattggatt tcactttcca
     5881 gacttgagcc ttatttttta gttttccccc tgctttattt gttgttacaa ctataacggt
     5941 tttattgtct ttgtttttct ttagcaagtt tgaattcaaa gggattctgc cttcggaatc
     6001 caaaacaatc ctcaagggat ttttgccttt cacctctctg gtggttaact tgggatcatc
     6061 ctttatgact gtatttactc ccactaacac ggcatccatt tgttttcgca gggagtgaac
     6121 aaatcttcga gaatctttct ggctaatcca tttcgaatct ccatcaagtg tagctatttt
     6181 accatctaag gtttgaacta tcttcagcgc tacaaaagga aacccacttg taatccgctt
     6241 aaaatagatt tcattcaact ttcttgcttc gtcttctaat acgccgagct taatttttat
     6301 tccggcttgc ctgagttgct taaccccggc tccattcacc ttcggatttg ggtcaatagt
     6361 ggcacagata acctctctta ttccatttct tattatacta tcaacgcagg gtggggttct
     6421 accaaaatga gaacagggct ctaaattgac atataatgtc gctcccttgc ctttctttcc
     6481 tgcagatttt aaggcatttg tctcggcatg aggactgcct attttttgat ggtaacccgt
     6541 tccaacgatt ttgccatttt tgactatcac tgctccaacc agaggattgg gggaagttct
     6601 gcctactcct tttttggcta atcttaaaac catttccata tatttttcat ccatagtcaa
     6661 tggtctttaa aaaaaaatcc ccgatcacca tcggggtata tttacacctt ttctcttaca
     6721 ccctctttcg tccggacttt gaccccgact aactacagtc gggacgaacc gtcggcactg
     6781 gaaattcacc agttcaaccc atatgggtgc gtgggctata ccaccgattg aggaatttca
     6841 cctcaccccg aaggcaaatg ccaatatata ttttattccg tctatgtcaa gtattttgtg
     6901 attttggtag aagttctctt cccctttaat cgctaaagtg gggagacctt gttctctatg
     6961 ctttgataat gctgctgtaa tatgcgacgt tatcgatacc gaaattcctg atattttcaa
     7021 tgtccatatc ccatttcata ggattattgg caatgtactg ccgaatcgat tccaaagatt
     7081 ttgcactgcg gattacatga tcatagaatg atttctgcca atgaaattgt aatgcaccgg
     7141 taccatgaat caattttgat gatgttgttt taaatgcgcc gatcaattcg ggcaatggtt
     7201 taatcttttg tagggaacgg tcgcgaccgt cccctacaac acaaccatcc atgcgaccat
     7261 tccctacgat tgaaatcatc ccatggaaat gattgggcat gacaacctgt tcgtccaata
     7321 ccacatatgg atattgatcg cccaaccatg cccattgctt ctccacaatt tccccacatt
     7381 cattcaaaac cattttcccg ttcttgatat tgccgaacca ttcgatgcgg ttgtgtatac
     7441 acacggttac aaaatacata ccattttgcg aataatcata acctttcaaa cggtttggtt
     7501 ttccatttcg catttttgaa ttacttcttt gacaatatca agtcaaagca aagatatccc
     7561 ataaatccta tttcaatcga ccgttctgca gacgatctag taccttttca aacaatttaa
     7621 atcttttcca caaaaaacct cgataatcac gggcaaactg tttctgtctt actcttagcc
     7681 ccctgtattt gaatgctcgc tcatattttt tgagttcata ggtcaggaga cccgaagcta
     7741 cccgcccggc aatcctacca attcttcctt acctctttca cgccgactct atccactaaa
     7801 acgatagcat cattgggaca ggcggctctg caaacgccac acccatagca atgattgaca
     7861 tctatgaaaa cgcgctctct tgccacactg tatcccatcg ccccgaactg gcacatctgc
     7921 atacaagacc tgcaaccact acatttttcc caatcaactc ttgctacata ttctgcctta
     7981 aacataaatt ttatatcata atttaaagtc gaaatcatag ccaaacagtc tgaacggtcg
     8041 caattgcata aaccgccgat aaacggagta ataaaagtcc agatggaatg gactgatcct
     8101 ttagtttcaa actcttttat tagctttaag gcgtccgact tctccatttt ctctaatcct
     8161 tgagcatacg gaccaagaag ataacccgaa tcgattttat cctctttcct agcccaggcc
     8221 ggcagagcac ttataccaaa gcaaaacctt tcttcttttc cagtcaaggt cttccgacaa
     8281 atgcagggca aacgcacaat tgaatttata atctcgaaga ttttttccac gtcttcaatt
     8341 ggcacaactt gtccgaaatg ctctttttta tacttgcggg ttatactgcc actgattaaa
     8401 ctctgaataa tccgaggggc ggttttcaat ttcttcatct gattcaagga ctcttcggca
     8461 gattccccag ccgttccgaa aaagccggaa atcattttct tgcgtctggt gtcactggct
     8521 aaatccaagc cgtaattttt agcctgtaaa taccatttct taccttcacc atgcttggta
     8581 cagaattcac acataggtta tttgggttat aaggttattt atgatatttt gtgcctttaa
     8641 ttatcgagaa agccctgtaa atctgctcca agagaataat ccaggtcaat tgatgagcaa
     8701 aggtcattct ggataaagat agtttaaagt tgctcaactt ttttatatcc tctgagagtc
     8761 ccaaggaact gccaaccaca aaggtgaagt cgctctggcc ttgatttatc ttgtttttga
     8821 aaaaccgagc taaatcttcg gactctaact catttccttt gttgtctaaa actattaaaa
     8881 gggttttatc ttttaagctc ttgattattt tattcttctc tttgtctaaa atattcaaga
     8941 cacttgagcg ttctgtaacc ttttcgggct ggatgatttt cacatctaaa tcgaccatgc
     9001 ccccaagcag ctttttataa tgatttacac cctctataat ccacttttct ttttgtcttc
     9061 ctaagaatat gaatcgaatc ttaaccaatt taatctgcta tttggttttg attattgtta
     9121 tccagacttt tctgcttctt ggcccatcga actcacaaaa atatatgcct tgccaggtcc
     9181 ccaatttcaa actgccatcc tgaatgatta tgctcctgga agcacctatt atgctcgatt
     9241 ttatatgtgc tgcggaattc ccctcagtgt gagagtagtt atcattgaag ggaacgagct
     9301 tattcagctc atatatgata tctctctgca cgctggggtc cgcattttcg tttatagtga
     9361 ctgcggcggt agtatgtgga acaaagatga tagagattcc tgattcaact ttgctggcag
     9421 atataacctc ttccacttta ctggtgatgt ccaccagttc aactctggat gaggtgcgaa
     9481 ttggtatttc cttaatcact gaagatcgcc tccacaaaat cctggggttt aaattcttct
     9541 aaatcttcca tcttttctcc gatgccaaca tatttaatgg ggatcttaaa cttatccgca
     9601 atggcaatta caattccccc tttggctgtt ccatccaact tagccaaaac tattccacta
     9661 agaccgaccg cttcatcgaa gagcttaacc tgagataggg cattctgacc aaccgttgca
     9721 tccagaacca aaagtatctc ttgcggagag ccgggcagaa ccttgcccat cacttttttt
     9781 attttcttaa gctcttccat taaactcgat ttggtatgca atctccctgc agtatcaact
     9841 ataagcacat ccgccttgcg ggcaagcgtt gcggttgttg catcgaaagc cactgctgcc
     9901 gggtctcctc ctggaacagc ctttaccata tccactccca ccctatctgc ccaaatggct
     9961 aattgctcaa tagctgccgc tcggaaagtg tcgcagccag ccaagagcac tttcttgtgc
    10021 tcctttttgt acctttgagc taacttggca atagtagtgg ttttgccagt tccatttacc
    10081 cccaccatca ttattatcca aggggacttg actttggatt cttctctata agaaatggta
    10141 tcaaaaaccg cgatcatctg ttttttcaga gtatcaatca catcctcttc gcttctgata
    10201 ttttcctgct tggctttcct ttcaagctgg cttgttatct gtgaggttac ctctggtccc
    10261 acgtctgctt ttatcagaat ttcccctatt ttttccagca cttcatccga gatgccctta
    10321 tgaagtccaa gcacctcctt tattttgagg aagaggcttt tcttggttct ctcaagtccg
    10381 gtctttaagt ttttgtgcga attctgcatc ttgagaatgg gataattcaa gcttggtctc
    10441 cagccaatac ctcttgacga ccgagtttaa ctgagacgat tttggaaact cccggctgct
    10501 ccatagttac gccatagagc gtatccgcag attccatggt cagcttatta tgggtgataa
    10561 ccaaaaactg ggtattctga ctgaaatgtc taatcatctt caagaatctc ttgacgttgg
    10621 aatcatctaa gggtgcatct atctcgtcca ggatgcaaaa tgggctgggc ttgaccagat
    10681 atatggcaaa aatgagagaa gtagctgtga gagctttttc tccaccggaa agctgattta
    10741 tgttgactaa tcttttgcct ctgggtcggg ctgatatttc gattggggat tcgagcacat
    10801 ctatatccgg ctctagggtc acatccgctt cacccccctc aaaaagctgc gcaaaaactg
    10861 attgaaaatt ggctctcacc ttctggaaag tctccaagaa catctctctg gcagtctcat
    10921 ctatcttgcc tatggttaaa ttcaaagagt ttttggcttc caccaaatcc tttatttgat
    10981 ttttgagaaa atccaatctc tccttggaga ctttatattc ttccaaagca accaagttaa
    11041 ccggacctaa acgggaaacc aactctctta agctctttat tctttgtctg taattttcca
    11101 gaccacctct ttcttcaaga gagagtggct ctaaatcctc cagattaacc tggtgttcct
    11161 cccaagcctt atcccgaatg cttctcatac gggtggcttt ttccagtttt cccaattcca
    11221 agttatgcac cttactctga aattcctctc taatctggcg aatggatttt aactgacttt
    11281 ccctggaaac tacctcctgc tgtaactggc tatatttgcc ctgccaggaa ttgtatatct
    11341 tctcgtcagt ctccttctgt tcaaacaagc ctttcagctc ctcatccaca ttggaattgt
    11401 tcagcgttat ctcctgaata cctttccttg agttttctgt ctgctctatt ctcatattta
    11461 tattcttcct gatgtcatca atcatctcat ttaagcgaag tagatcgttt tcgagctgct
    11521 ccttcttgcc actaagggag actaaatcta tcctgaagct gttcgtcttc tcaaactgga
    11581 gattcctttc ttcttcaatc cgatccaatt cttctttggt ttcgaacacc tccttgctta
    11641 gctcggaatt atcgcgctcc aacgcttcga gctctagttt catctgcttg atagagcgtt
    11701 caagctcccc tgcctcccct tgaagtttgt ttaattcctt tatattcatc tcaacccttt
    11761 gcgtcaattc cctgctctca aactccttct gcttaagctc catatccttt tggctaagct
    11821 cctctttaag tcggcttata tggctaatcc tctcggataa ggactgattc aaactcccaa
    11881 gaaaatcttc ctttttttgt ttttcttgct gtattccttt aaactcattt cccagaatag
    11941 ataccttttc tgctatcccc tgcacctgct tttccctgcc taacagatgt acttcctttc
    12001 tcgagccgcc ttccagtacc ccctcaggtc ttataacttt tccactcaag ctaaccaaag
    12061 tgagattctc ttttagtgta gaagccatct tctgtgcgcg ctcaaaggaa tccaccacca
    12121 aaacatctgc caacagaaac ataacatccg gcttatatct ctcttcgcaa cgaaccaagt
    12181 taaaagccca ccccattacc ccttcatgct ctttgatact gactgtcttg ttctgctcag
    12241 tcactttttt gactttctcc aaaatcagaa aactcgcccg accctccttg tgttcctcaa
    12301 gaaatcttat ggcacgcaag gcagaagctt catccccgca tattaaaaat tgcgctcttt
    12361 cccctaaagc cgattcaatg gctgaaatgt acttctcgtc agtgttgatt aaattcgcca
    12421 ctgaatcaat tacgcctggc aaagaggagc gctcatccag aatgtatttc actccatcct
    12481 gaaacccctg atattccttt attatctcct gaaaaagggt taacctagct ttctcggaag
    12541 acagttcatt gcctatctta acctcttgtt ttgaaattct ttccagctcc tcctcattta
    12601 ttttgatttc ttgttccagt ttattctttt ctgcattttc tccttctagg gctatccttt
    12661 tttgctcgca ggctgacgag agtgcattaa tttgagactt aatttcattt aggcggtcgg
    12721 agaggacatc ttcttcttgt cccagatttt cccttctctg ggttagctgt ttgaaactac
    12781 cctctaagtt ggctactttg cttttcctct cacttgtctc ttcttccttg cttgcctctt
    12841 gcttttccaa atcctctaag attgccctga ccttgtccct cttttcatcc acaatcgaaa
    12901 gctcattttc cttctggcta agctcccttt ctttctcctt tagcttttga gtggtgactg
    12961 caagctcctc ttgttttgcc gacgtttcat tctcgaacag ctttagccga ttctctaaat
    13021 cgctcaattc ctgcttcaac tgggttattg atttctctaa gctggctctt ctctcttctc
    13081 gcaccaaaac atcccgctcc acctgatgag ccttctccac atccgaagca agtttctttt
    13141 gataagtcac cagttccttt tccgtctcca gaagctccag tcttaagcgc tccacttctg
    13201 cttcctgctt ggacaaatcg gttgtgtgct ctcttgtcct cttcttgagc tcaagcaggt
    13261 cttgttccaa cctatcattc tcagccttca aatccccgaa atcagcttga gccagtttta
    13321 tttccagttg agttgcttcc tcagagaact ttttgaaccg ctccgccctt ctcatctgcc
    13381 tctttagcga actaacctgc ctttccactt cagttattat gtcgttcact ctaaggatat
    13441 ccccttccgt gatctcgagc ttgcgtatgg tagctttctt acgtagttta tatttggtga
    13501 tgccgctggc ttcctcgaac aataaccttc tatcctctgc cttgtccgaa agcacagctt
    13561 ctatcattgc tggttgaata accgagtagg catgcgttcc catcccggta tcgaggaaca
    13621 agtcaatgat gtcctttagc cggcagggag ctccattcaa aagatactca ctttctccag
    13681 aacgatagag cctgcgggta acgcgaactt cgctatattc gatgggcaat atccccttgt
    13741 gattctttac gacaagggag acttctgcca tacctaacgg tttgagctcc ttggtgccat
    13801 tgaatatgat ctcttccatc ttggagccgc ggagaagggt aactctctgt tcccctaaaa
    13861 cccatctaac cgcatccagc aggttggttt tgccacatcc atttgggccc acaatcgcag
    13921 ttacaccatt gtcaaaagcc aattcggttt tgtcaggaaa agatttaaag cccagaagtt
    13981 ccagcttgga tagatacaat tctaactcct tctttgagcc cctgttccgt ttcttaaaaa
    14041 ccttagggtc tccagcatgg catcatatct ttggtccaga taaaccaacc ccttctgagt
    14101 caccagccga atgttgtaaa atggtttccc ccatttcccc tccattccaa tacgcaatgg
    14161 cgttccactg gaaaagcagg ttaagacatt gaaaaggcaa ccttttaaat ccaggtcaat
    14221 ggctatgcca aaatcatgct cttttatctt ttggatgaat ttgcgactgg gcaaggaaaa
    14281 tggcttcaag tcagctggac gcggcgagat gatctcataa gatttacaat tggcaataaa
    14341 actatccgct tctaataacg gaagcaaaag gtatatcttg gaagaagaaa agatatctgc
    14401 taatgcaggt agatgattgg ccgctgaggc gaattcctct ttggaagcag ggaaacaaac
    14461 caaaaccgat ttggcagtgg aaaaagaggg gtgaaatgaa aaggatgatg atgcgccaaa
    14521 tcgtctcttc caccaaacca ccagattctt caccaagaaa tttgtcctta ttttcttttc
    14581 cacaagtaca tctactgtaa atattggttg tagttttggc gatttatctc agctttgaag
    14641 attcttcttt gtggctgact gaaagcttcg agtttgctcc catttaggaa gacctccaca
    14701 gctgaaattt tgcccaagtt gataacgaaa cgctccttgg cttccacttc cagcttcgtt
    14761 cccggacgaa cctcacccga gaggactgtg tcgttatcag caatcaccaa gatccaggta
    14821 cgtttaactg gctttatctc cagaaccaac tgctccggca aaaccacttc ggcctgcatc
    14881 aaagattcag taactgcagt ttctggaacc aaagccaatt tttcaatagc ttgggtagtg
    14941 taatcaccgt agttgatatt ctcttgctga gcatattgaa caaatatcgc cactattact
    15001 atccccagaa ctaatccagc cgctatgaaa gcccaaccag tctcggattc tacagtttgg
    15061 ggacgcgact gctgttcaag ttcttctttc ttcttgtccc atccgtattc attcaacaat
    15121 tcttccgact ctaagcctaa gaaatgtgca tacgccttga cgaaaccttt ggcataaggt
    15181 tcgcttggaa gagcattcag attatcctcc tcaatggctt ccagatagtg cactcgtatt
    15241 ctggtttgat ctgctacctc cttcacggtg taacccaagc tctcccgatg tttcttcaga
    15301 tatgaaccca aactccccat ttctcacctc agcatatgaa ttaacatctc ctctaatttc
    15361 cccattggca gacctatgac gttgtccaaa gagcctttga cttcccgaac caagcgacta
    15421 ccccttccct gtattccgta tgccccagct ttatccgtag gctcaccggt cgagatatag
    15481 ctatctatac cttcagatga aagagagttg aatttaactt cagtgcattc gtaatctgaa
    15541 acggtcttct ttttgtcttt atctatcaag gtcaagccgg tatagacctt gtgctcccgg
    15601 ttgctcaact tctccaacat cctcttcgct tctttttgat tcttcggctt acccaaaatc
    15661 tctccgttca aaaccacaat cgtatccgca cccaaaacta aaccgccctc aacctcaggc
    15721 agcacgctct ccgctttcat cctggatagt cttaaaacat gctgcttggg attttttgat
    15781 acattttctt cattaatctt gggagtcttg acttcaaaat caatattcgc ctctttgaga
    15841 agttccatcc gacgaggtga cctcgaagct aaagtgaatt ttcttttgcc aataaaacca
    15901 gctaaatttg cctccgctcg aagtgtcatt tgctatctaa aatcagggtg accggtcccc
    15961 agttaatcag ctcaattgcc atcttggccc caaattcccc ttgctccact ttaagtctat
    16021 tttgccttaa catctggata aacttaacat aaagcttttg agctaaatca ggctccagtg
    16081 cacgagtgaa actaggtctt cttcccttct taacatcagc atacaaagta aactgtgaaa
    16141 ccaccagaag ctcagctcca acatctaaga cggataagtt catctttccc tcttggtctt
    16201 caaagattct caaatttgtg cacttatccg ccaagtactt tacatcttct tcattatctc
    16261 cttgcgttgc tcctaccaga atcagaagac ctctgtttat ctgggctatt gtcttccctt
    16321 caactgaaac tctggctcgg tttactcttt gtatcactgt cctcaaggta gtttccttag
    16381 ttaatgcaaa gaattaatat aaccgccctt aaaagtcaat agaaaagctc taggaagtat
    16441 tcccactttt tcaaaaaata catcagaatg tcttaatggc tatttcctcc agtttctctc
    16501 tccccttggg ggagagagat agagtgcctg cccggccagg gagggggaaa agaaaggaat
    16561 catcaccctt actccgcctt tggcgagatt ttcaactcta tcctctccct tgaagggaga
    16621 ggatagagaa gagtattcct ttcttaaaga taagaattgc cttgccccgt tttgggggat
    16681 actcctgaaa agccctgctt cacgcttgat aaaggccagt cacttccaag ttttcgtccg
    16741 gagataatat tctgtattta tccacaggta aatttctatc ctcaattatc tctatctcca
    16801 atttgagttc tcttctcaag ttttttatgc ggtcttctct ttccctactg aaaagcacat
    16861 aaagctgagg gcttgctact atttgatatc tcccaactga agaagctaac ttagctctcc
    16921 tgaaccatcg ctccacctta gttgccaagg tttcaactga aaggattcta cccacacccc
    16981 tgcaagtagg acaaggctca ctgaaagcat acattaaact gggcctaatt ttttccctgg
    17041 tcatctcaat tagtccgaat tcatccaccg gcaagatgct gcttttggag cgatcactgc
    17101 gcatactctt cttgaactcc tcgtaaagct tttttctatt ctctcttgaa tccatgtcga
    17161 tgaagtcaat aactatcaaa cctccaatat ctcttagtct tatctgtcgg gctatctctt
    17221 tggctgcttc gaggttcact ttcaggatgg ttgcctcctg gtcgctcttg cccacatatc
    17281 tgccggtgtt gacatcgatg gtcaccatag cttcagtctg gtcaataact atataagacc
    17341 ctttcttgat ccagactttt ctatcaagca ttttatctat ctcggattct attttgtaga
    17401 aatcgaaaag ggggaccttc ccctgataga gcttcacctt ggagcgcaag ttaggtgcaa
    17461 ttgaacgcaa ataagagagt atgttatgat actccctctt ggaatcgaca accacgctgg
    17521 tgatctccgg ggtcaaaagg tcgcgcatta tgctggaggt gattcccatc tccttatgaa
    17581 gcagatacgg agcttttttc ctttgggctt ccttttttat cttctcccaa agtcgcaata
    17641 gatttttgac gtcggacctg agttccctct cagtctttcc ttcagccact gtcctgacta
    17701 tcaaacaaca atcttttggt ttgagttgag aggctaaacc tttcagtctc tttttttcag
    17761 cccgattgga gatctttcga gaaaccctta ccccttcctc ctccggcacc aaaaccgaat
    17821 acctgcccgg aatggaaagt tccgaagtga ctcttggtcc cttggttccc atcggctctt
    17881 ttattatctg aaccaatatc tc
//
LOCUS       DASWLN010000098         3885 bp    DNA     linear   ENV 20-FEB-2024
DEFINITION  MAG TPA_asm: Candidatus Zixiibacteriota bacterium isolate
            SMAG_U2949 k141_599457, whole genome shotgun sequence.
ACCESSION   DASWLN010000098 DASWLN010000000
VERSION     DASWLN010000098.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37960594
            Sequence Read Archive: SRR10376091
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Candidatus Zixiibacteriota bacterium (soil metagenome)
  ORGANISM  Candidatus Zixiibacteriota bacterium
            Bacteria.
REFERENCE   1  (bases 1 to 3885)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 3885)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (26-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/12/2024 08:28:26
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 1,440
            CDSs (total)                      :: 1,416
            Genes (coding)                    :: 1,412
            CDSs (with protein)               :: 1,412
            Genes (RNA)                       :: 24
            rRNAs                             :: 1 (16S)
            partial rRNAs                     :: 1 (16S)
            tRNAs                             :: 21
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 4
            CDSs (without protein)            :: 4
            Pseudo Genes (ambiguous residues) :: 0 of 4
            Pseudo Genes (frameshifted)       :: 0 of 4
            Pseudo Genes (incomplete)         :: 3 of 4
            Pseudo Genes (internal stop)      :: 1 of 4
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..3885
                     /organism="Candidatus Zixiibacteriota bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_599457"
                     /isolate="SMAG_U2949"
                     /isolation_source="soil"
                     /db_xref="taxon:2053527"
                     /environmental_sample
                     /country="USA: Oregon"
                     /lat_lon="42.99 N 123.63 W"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            complement(<1..126)
                     /locus_tag="VGB16_03275"
     CDS             complement(<1..126)
                     /locus_tag="VGB16_03275"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_018200440.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008685 - 2-C-methyl-D-erythritol
                     2,4-cyclodiphosphate synthase activity [Evidence IEA];
                     GO_process: GO:0016114 - terpenoid biosynthetic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="2-C-methyl-D-erythritol 2,4-cyclodiphosphate
                     synthase"
                     /protein_id="HEX9916735.1"
                     /translation="MYRIGIGYDAHKLVKNRNLILGGVRIPYSKGLLGHSDADVLS"
     gene            complement(250..936)
                     /gene="ispD"
                     /locus_tag="VGB16_03280"
     CDS             complement(250..936)
                     /gene="ispD"
                     /locus_tag="VGB16_03280"
                     /EC_number="2.7.7.60"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_015310873.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0050518 - 2-C-methyl-D-erythritol
                     4-phosphate cytidylyltransferase activity [Evidence IEA];
                     GO_process: GO:0019288 - isopentenyl diphosphate
                     biosynthetic process, methylerythritol 4-phosphate pathway
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="2-C-methyl-D-erythritol 4-phosphate
                     cytidylyltransferase"
                     /protein_id="HEX9916736.1"
                     /translation="MKTVAIIVAAGKGERIKSKLPKQFLNLGKKPILAHTIEKFEKSR
                     LVDEIVVVVPKDYLSFCSKEIVDRFGFGKIGRIVSGGKERQDSVFRGLKAINSTMNIV
                     LIHDGVRPLVKTQKINKIIEVCRKEGPVIFALPVKETIKRVEKNRVITTLDRNKIWAV
                     QTPQAFSYQLIMHAYKEANKDKFIGTDDSQLVERMGIAVKVIKGDENNIKITTMDDLK
                     IAEYLVSLRD"
     gene            complement(949..1980)
                     /gene="queA"
                     /locus_tag="VGB16_03285"
     CDS             complement(949..1980)
                     /gene="queA"
                     /locus_tag="VGB16_03285"
                     /EC_number="2.4.99.17"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_010233520.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016740 - transferase activity [Evidence
                     IEA];
                     GO_function: GO:0016853 - isomerase activity [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="tRNA preQ1(34) S-adenosylmethionine
                     ribosyltransferase-isomerase QueA"
                     /protein_id="HEX9916737.1"
                     /translation="MKTIDFDYNLPKELVAQFPSKERDQSRLMILNRAEKKVEHKVFN
                     QIVDYLQPLDGLVINQTKVFPARLMGQEKNSKVKVEVMLLKQSTPDSWEALVRPGRKG
                     KLGEQIIFGNDGLCCEIEEELTLGKRLVKFNFEGDFVEILNRLGRVPLPPYIKREPEK
                     EDEDRYQTVYAKDRGAVAAPTAGLHFTSDLLDKIRDMGVKIIPITLHVGLDSFRPIRE
                     EDYRKHRLESEYFHLEPSSADDINEIKSKAGRIIAVGTTTTRCLETVSDSSGRVYAQN
                     GWTDKFIYPPYEFKVVDALITNFHLPKSTLLLLVSAFAGREFILRVYQEAMKQKYRFY
                     SYGDAMFIF"
     gene            complement(1977..2303)
                     /locus_tag="VGB16_03290"
     CDS             complement(1977..2303)
                     /locus_tag="VGB16_03290"
                     /inference="COORDINATES: protein motif:HMM:NF022596.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF2905 domain-containing protein"
                     /protein_id="HEX9916738.1"
                     /translation="MLASVGKTLILMGLIFLILGGIFVISGKIPFLGKLPGDIQIQKE
                     NFGFYFPLGTSILLSIILSILLASPKFRSPLGEAEGLLYLGDPVIYHGEASLSVILIL
                     YNLFKR"
     gene            complement(2322..2849)
                     /locus_tag="VGB16_03295"
     CDS             complement(2322..2849)
                     /locus_tag="VGB16_03295"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_012531783.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="epoxyqueuosine reductase QueH"
                     /protein_id="HEX9916739.1"
                     /translation="MKLLLHICCSCCLTYPFEKYKKNGVDLVGYWYNPNIHPYTEYRN
                     RLKALKGFAKDVSFPVTYDESYELEKFLKGAMKDLNNRCLNCYLLRLEKTAKHAKEKG
                     FDFFSTTLLISPHQKHKLIKEVGEKIADEVGVDFYYDDLRKGYDQARKMTREKGLYSQ
                     KYCGCIFSEKERYLD"
     gene            complement(2858..3877)
                     /gene="ruvB"
                     /locus_tag="VGB16_03300"
     CDS             complement(2858..3877)
                     /gene="ruvB"
                     /locus_tag="VGB16_03300"
                     /EC_number="3.6.4.12"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_020252943.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003677 - DNA binding [Evidence IEA];
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_function: GO:0009378 - four-way junction helicase
                     activity [Evidence IEA];
                     GO_process: GO:0006281 - DNA repair [Evidence IEA];
                     GO_process: GO:0006310 - DNA recombination [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="Holliday junction branch migration DNA helicase
                     RuvB"
                     /protein_id="HEX9916740.1"
                     /translation="MKERVTFPEKLSDEIEYDITLRPSSFDEFIGQEKLKDNLKIFIQ
                     AAKKRKEALEHVLFYGPPGLGKTTLANILSKETKVNIKATSGPVLERPADLAGILTNL
                     QGKEILFIDEIHRLSHVVEEYLYPAMEDFSLDIMIDKGPSARSVKLALKKFTLIGATT
                     RAGLLTSPLRSRFGVVCRLDFYEPEDLYKIVLRSAKILGVEIDEEGARQIAKRSRGTP
                     RVANRLLRRVRDYAQVKAKGKIDKNIAIDALSMLNVDELGLDEMDKKIMEVVIHKFKG
                     GPVGINSLSVAVGEEEETIEEIYEPFLIQCGLIDRTPRGRFATELAYKHLGIKCDTGK
                     QGKLL"
BASE COUNT         1026 a          950 c          666 g         1243 t
ORIGIN      
        1 tgacaggacg tctgcgtctg aatgccccaa gagaccctta gaataaggaa ttcttacacc
       61 gcccagaatc aaattcctgt ttttcaccag tttatgagca tcgtatccga ttccgattct
      121 atacaaaagc tctcctttca gaaaagttca tcctctccct aaccttctct ttttcaagga
      181 gagggaatgg gtgaggttct tacaagtttc gaacatgtct agtggtcaag attgccgtta
      241 ctctccatct taatccctca gacttactaa atattctgct atcttcaaat catccatcgt
      301 ggttatctta atgttattct catctccttt tatgactttg accgctatcc ccattctttc
      361 aaccaattgg gaatcatctg tgccaatgaa tttatctttg tttgcctctt tataggcgtg
      421 catgataagc tgataagaaa acgcctgtgg tgtctgcact gcccagattt tatttctatc
      481 caaagtggtt ataaccctat ttttctctac tctctttata gtttccttga ctggtagggc
      541 aaaaattacc ggtccctctt ttcgacagac ttctatgatt ttgtttatct tctgggtctt
      601 caccaagggc cttactccgt cgtgaatcag gacgatattc attgtggaat ttatggcttt
      661 aagtcccctg aaaactgagt cctgcctttc cttccctcca ctcacaatcc ttccaatctt
      721 accgaatcca aatctatcta cgatctcttt tgaacaaaaa ctcaaataat ctttgggtac
      781 aactaccact atttcatcca caaggcgaga tttttcgaat ttctcaatgg tatgcgccag
      841 tatcggcttt ttaccgagat tcaaaaactg tttagggagt ttacttttga tcctctcccc
      901 tttacccgcg gcaactatga tagctactgt tttcaagtct ttctcctttt agaaaatgaa
      961 catcgcatca ccgtagctat aaaaacggta tttctgcttc attgcttctt ggtaaacccg
     1021 tagaataaat tcccttccgg caaaagccga gaccaaaaga agcaaggtgc ttttgggaag
     1081 gtgaaaatta gttatcaagg cgtccaccac tttgaactcg taaggaggat agatgaattt
     1141 gtcggtccag ccgttttggg cataaactct gccggaagag tccgagactg tctccaggca
     1201 tctggttgta gttgttccca ctgcgattat ccgacccgct ttgcttttga tttcatttat
     1261 atcgtcagct gaacttggtt caagatgaaa atactcagat tctaatctat gtttcctgta
     1321 atcctcttct ctgattggac gaaaactatc caaccccaca tgcaaagtta tggggattat
     1381 cttaactccc atatccctta tcttgtccaa caaatctgag gtgaaatgca atcctgcggt
     1441 tggagcggca accgcccctc tgtctttagc gtaaacagtc tgatacctat cctcatcctc
     1501 cttttcaggc tcccttttta tatagggagg aagcgggact ctgcccaatc tgttcagtat
     1561 ctcgacaaaa tcaccttcaa aattaaactt caccagtctc tttccgagag tcaattcttc
     1621 ctctatttca cagcataacc cgtcattgcc aaagattatt tgttctccca atttgccctt
     1681 tcttcccggt ctgactaatg cttcccaact gtctggtgtg ctttgcttta agagcattac
     1741 ctcgactttt actttactat ttttctcttg gcccataagt ctcgccggaa agactttggt
     1801 ctggtttatg accagaccat ctaaaggttg taaataatcc acaatttgat taaaaacctt
     1861 atgctcaacc ttcttctccg cccgattcag tatcataagc ctagactggt ccctttcctt
     1921 ggatggaaat tgagctacaa gctctttagg caagttgtaa tcgaagtcta tggttttcat
     1981 cttttgaaaa gattataaag aatcaagatg actgaaagtg aagcctcccc gtgataaatc
     2041 acggggtctc ccaaatataa aaggccctcg gcttcgccga gaggcgagcg aaacttcggc
     2101 gaggcgagca aaatagataa aattatgctc aaaaggatgc tagtccctaa tggaaaataa
     2161 aagccgaaat tctccttttg aatctgaatg tctccgggca gttttcccaa aaaggggatt
     2221 ttacccgaaa tcacaaaaat cccgcccaat attaaaaaaa ttaaacccat caaaatcagg
     2281 gtcttgccaa cgcttgctaa catatggatt tgtctaaaac cttagtccaa gtaacgttcc
     2341 ttctcactga atatgcaacc gcaatacttt tgtgaatata atcctttctc cctggtcatt
     2401 ttcctggctt ggtcataacc ttttcttaaa tcatcatagt aaaaatctac ccctacctca
     2461 tccgcaatct tttctccaac ttcttttatc agtttgtgct tttggtgagg acttatcaaa
     2521 agagtggtgc taaagaaatc aaaccctttt tctttggcat gctttgctgt tttctctaac
     2581 cttaatagat agcagttaag acatctatta ttcaaatcct tcatggctcc tttgagaaat
     2641 ttctccaatt cgtatgattc atcgtatgtc acgggaaaag agacgtcttt cgcaaacccc
     2701 tttaaagctt taagtctatt gcgatattcg gtatagggat ggatattggg attataccaa
     2761 tacccgacta aatcaactcc attcttttta tatttttcaa aagggtaggt taaacaacag
     2821 gaacagcaga tgtgcagaag aagcttcata tttcaaatta gagcaacttg ccctgttttc
     2881 cagtatcgca tttgattccc agatgtttgt aggctaactc cgtggcaaag cggccacgcg
     2941 gggttctatc aattaagccg cattgaatca agaatggctc atatatctcc tctattgttt
     3001 cctcctcttc gcccaccgcc acagataagc tatttattcc tactggacca cccttgaatt
     3061 tgtgaatgac tacctccatt atcttcttat ccatctcatc taagcccaat tcatccacat
     3121 tcaacatact taaagcatct attgctatgt tcttatctat ctttcccttg gctttcacct
     3181 gtgcataatc cctgaccctg cgcagaaggc gattagccac acgcggagtc cctctcgatc
     3241 ttttggcaat ttgtcttgcc ccttcctcat ctatttccac tcccaatatc ttagctgacc
     3301 ttaatactat tttatatagg tcttccggct cgtaaaaatc cagtctgcat accactccaa
     3361 accttgatct caagggggaa gtcaaaagac ctgctcgcgt ggtggcgcca atcagagtaa
     3421 attttttcaa agccagcttg accgaccggg cggaaggccc tttgtctatc atgatatcca
     3481 gagagaaatc ctccatggcc ggataaagat attcctccac aacgtgactt aagcgatgaa
     3541 tttcatctat aaacagaatt tcctttccct gaagattagt caaaatgccg gctaaatcag
     3601 ctggtctttc cagtaccgga cctgaagtcg ccttgatgtt gactttggtt tccttgctta
     3661 agatatttgc caaagtggtc ttgcccaatc ctggaggacc gtagaacaaa acatgctcca
     3721 aagcctcttt ccttttcttg gctgcctgaa tgaaaatctt gaggttgtcc ttcagcttct
     3781 cctgacctat gaactcgtca aaagacgaag gcctgagagt tatatcatat tctatttcat
     3841 ctgataactt ctctggaaaa gtgactcttt ccttcaattt cttat
//
LOCUS       DASWLN010000099        13448 bp    DNA     linear   ENV 20-FEB-2024
DEFINITION  MAG TPA_asm: Candidatus Zixiibacteriota bacterium isolate
            SMAG_U2949 k141_343725, whole genome shotgun sequence.
ACCESSION   DASWLN010000099 DASWLN010000000
VERSION     DASWLN010000099.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37960594
            Sequence Read Archive: SRR10376091
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Candidatus Zixiibacteriota bacterium (soil metagenome)
  ORGANISM  Candidatus Zixiibacteriota bacterium
            Bacteria.
REFERENCE   1  (bases 1 to 13448)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 13448)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (26-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/12/2024 08:28:26
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 1,440
            CDSs (total)                      :: 1,416
            Genes (coding)                    :: 1,412
            CDSs (with protein)               :: 1,412
            Genes (RNA)                       :: 24
            rRNAs                             :: 1 (16S)
            partial rRNAs                     :: 1 (16S)
            tRNAs                             :: 21
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 4
            CDSs (without protein)            :: 4
            Pseudo Genes (ambiguous residues) :: 0 of 4
            Pseudo Genes (frameshifted)       :: 0 of 4
            Pseudo Genes (incomplete)         :: 3 of 4
            Pseudo Genes (internal stop)      :: 1 of 4
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..13448
                     /organism="Candidatus Zixiibacteriota bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_343725"
                     /isolate="SMAG_U2949"
                     /isolation_source="soil"
                     /db_xref="taxon:2053527"
                     /environmental_sample
                     /country="USA: Oregon"
                     /lat_lon="42.99 N 123.63 W"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            complement(<1..1232)
                     /locus_tag="VGB16_03305"
     CDS             complement(<1..1232)
                     /locus_tag="VGB16_03305"
                     /inference="COORDINATES: protein motif:HMM:NF014757.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="3-hydroxyacyl-CoA dehydrogenase family protein"
                     /protein_id="HEX9916741.1"
                     /translation="MNLDEKIRKVSIVGAAGKMGSGIALLLADQMAKEKLNPKNKDFA
                     YRLNLIDVNDDALDGLVKYLKTQLVKAGEKGAVTLRELYKDRLDLIENFDIVNQFVED
                     AASLVRCSTDLNLAKGSNMVFEAVLEDKDLKVNLLKDLDKICGKEVVYLTNTSSIPIN
                     ILNKEANLNGRIIGFHFYNPPAVQRLAELICAKETKPELKDLALELAKRLRKKIVPSN
                     DIAGFVGNGHFLRDGLYGVREAKRLSRELGLAGGIYAVNKVSQDLLVRPMGIFQLIDY
                     VGVDVFHCISETVSQYLKGEDLKDDLLARMVQKRVLGGQRSDGTQKDGFLKYEKNRPV
                     GVYDIEKGEYKMFDSNAWAKELDKKLGDYPGGFFAWKALLVDPKKDEKLSTYFKNLNS
                     SSSLGAKLALSYLKNSKE"
     gene            complement(1269..1781)
                     /locus_tag="VGB16_03310"
     CDS             complement(1269..1781)
                     /locus_tag="VGB16_03310"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEX9916742.1"
                     /translation="MFKTSLALIVLLIILSTTCYGGEIVLKKSFWWGYSYTKDELQHQ
                     VGDGLRLKQNMAGVKEAQVEIDKYRRRRSLYIWFDLIRWPPLAVSYGRAFARRSFETD
                     DTQTFLLISTLATLVAGQLLYHSANSHLKKGVGIYNDSLKQQQANTQRLDFMVTPRSG
                     GYSFAMKYSF"
     gene            complement(2139..2645)
                     /locus_tag="VGB16_03315"
     CDS             complement(2139..2645)
                     /locus_tag="VGB16_03315"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_013374818.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="biotin/lipoyl-containing protein"
                     /protein_id="HEX9916743.1"
                     /translation="MEHEFKIQDKTYKLKIESKGNSYKIELDGKRYDIDAELISSNSI
                     SFLMDGQALTAHLAERDGKFLVNINGESFCIEKPEEKKRKNFAGEHTHVGEKNFVSTP
                     MPGKIVKISVKVGDEVRKNQTLVIVEAMKMENEIKSPIPAKVKKINFKEGDLVDTGQP
                     IVELEPKE"
     gene            complement(2646..4148)
                     /gene="accC"
                     /locus_tag="VGB16_03320"
     CDS             complement(2646..4148)
                     /gene="accC"
                     /locus_tag="VGB16_03320"
                     /EC_number="6.3.4.14"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_008604846.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0009317 - acetyl-CoA carboxylase complex
                     [Evidence IEA];
                     GO_function: GO:0003989 - acetyl-CoA carboxylase activity
                     [Evidence IEA];
                     GO_function: GO:0004075 - biotin carboxylase activity
                     [Evidence IEA];
                     GO_process: GO:0006633 - fatty acid biosynthetic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="acetyl-CoA carboxylase biotin carboxylase
                     subunit"
                     /protein_id="HEX9916744.1"
                     /translation="MFNKILIANRGEIAVRVIKACKELGIPTVAIFSTVDRNSLHVQM
                     ADEAVLVGPPAPLESYLNIDSIISIAKKLKADAIHPGYGFLAENPAFTERCEKEGIVF
                     IGSDSKSLALVGDKIQSRETMQRAGIPIIPGMKGSSKDINEFKKEAKQIGYPVILKAS
                     GGGGGKGMRKVRSEDELESSISAGMREARSAFGDDSVYLEKYIEHPRHIEFQVLADKF
                     GNAVHLFERECSIQRRHQKIVEETPSVALDDSLRKKMGETAVKAIMAANYSNAGTVEF
                     LLDEDKNFYFLEVNARIQVEHPITELVVGVDLVKAQIRLAAGEKMFLKQDQLKQRGHA
                     IECRIYAEDPENNFLPCSGKILFMKEPTGPGIRHDCGIYTGFEVSVYYDPILSKLVVW
                     AEDRPTAARRMLTALSDLTVLGIKTTTGFLKDVVSHSDFLEGKTTTDFIPRNFTDWKE
                     KEDNKKYLNQALIAAAIMSQNKFVSKGQAVEQESYSPWQALGKWEIGMRN"
     gene            complement(4396..5199)
                     /locus_tag="VGB16_03325"
     CDS             complement(4396..5199)
                     /locus_tag="VGB16_03325"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_008442110.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="enoyl-CoA hydratase/isomerase family protein"
                     /protein_id="HEX9916745.1"
                     /translation="MKGRKLEHYQTIIFKTEDAVANVILNRPEVHNAFNEVMIKELTE
                     VFEKISKDEHIRVVVLTGKGKSFCAGADLNWMKKVISFSYEENFDDSFKLAQLFYLIY
                     SLPKPTIAKVNGAAIGGGTGLVSVCDIVIASEEAKFSLSEVKLGLVPACISPYVIRRM
                     GESRCREFFLTGERLSAERASKFGLVNEVVPSNDLDKAVDEKVKQLISSGPEALKCCK
                     ELLEKVPLMNLDLATSYTAEVIAKLRISDEGQEGMNAFLEKRKPKWSKD"
     gene            complement(5374..6981)
                     /locus_tag="VGB16_03330"
     CDS             complement(5374..6981)
                     /locus_tag="VGB16_03330"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_013955262.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="carboxyl transferase domain-containing protein"
                     /protein_id="HEX9916746.1"
                     /translation="MFRIESKVDTQAIEFKENQKAMQKVVDQFKERLAKVKEGGPADM
                     KKRHKERGKLTARERLDLLFDKNSPFLELSALAAYDMYDNDAPSAGVITGIGVVHGRE
                     VLVVSNDATVKGGTYYPLTIKKHVRAQEIATENRLPCIYLVDSGGIFLPYQSGTFPDK
                     DHFGKIFYNQARMSAMGIPQIAVVLGSCTAGGAYVPAMSDEAVIVRKQGTIFIGGPPL
                     VKAATGEEVTDEELGGADVHCRISGVADHYAYNDEHALEITRNIVENLNPPEKFRLHT
                     SEAEEPYYDPKEIFGIVPRDIRKPYDIREIIARIVDKSEFQEFKELYATTIICGFARI
                     MGYPVGILGNNGVLFSESSLKAAHFIELCTMRKIPLIFLQNITGFIVGKQYEHGGIAK
                     DGAKMVHAVANADVPKFTVIVGGSYGAGNYGMCGRAYDPRLLWMWPNAKICVMGGEQA
                     ANVLLTVKKRQLEKKGIVMSKEEEEKFMAPTLEKYETESSPYFSTAHLWDDGIIDPLD
                     TRAVLGLGISMALNATIPDQRYGIFRM"
     gene            complement(7086..7475)
                     /locus_tag="VGB16_03335"
     CDS             complement(7086..7475)
                     /locus_tag="VGB16_03335"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_020263789.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="Zn-ribbon domain-containing OB-fold protein"
                     /protein_id="HEX9916747.1"
                     /translation="MLSARYWREMPQRYRLEAEKCKSCGKIYFPPRLICSACKSKGFE
                     KYKLKDTGKLVTYTIIRVGPSQFIDQIPYALGVVELEEGVKILAQITDCDLDKLSTGI
                     KLKIEFRKLQEDGKAGIICYGYKAVPI"
     gene            complement(7475..8635)
                     /locus_tag="VGB16_03340"
     CDS             complement(7475..8635)
                     /locus_tag="VGB16_03340"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011019746.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="thiolase domain-containing protein"
                     /protein_id="HEX9916748.1"
                     /translation="MREVCVIGLGMNKWGELWDKSLRDIFVEAALEAMDDAGVDKIDS
                     MYVGCMSSGLFVHQEHLGSLLADYLGVNPIPCTRVESACASGGAAVRAGFIEVASGIS
                     DIVLAGGVEKMTDVSVDGATFALATAADQEYEVYQGVTFPGLYAMMARAHMEKYGTTR
                     EQLAQVAVKNHKHGSMNPLAQFPMKITVEQVINSVMVADPLTVLDCSPITDGAAAIIL
                     CSLEKAKELGLKKPLVKIIGSAQATDTIALHSRDDLTFLKATQKAGEVAFKMANKKPS
                     DIHLAEVHDCFTIAELIVLESLGLTEKGEAGKATENGMTTLGGKIPINTSGGLKSKGH
                     PVGATGVAQIVEITKQLRGEAGKRQVKNAKVGLAQNMGGSGGSTVVHILEVA"
     gene            complement(8644..9690)
                     /locus_tag="VGB16_03345"
     CDS             complement(8644..9690)
                     /locus_tag="VGB16_03345"
                     /EC_number="2.3.3.10"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011405735.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004315 - 3-oxoacyl-[acyl-carrier-protein]
                     synthase activity [Evidence IEA];
                     GO_process: GO:0006633 - fatty acid biosynthetic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="hydroxymethylglutaryl-CoA synthase"
                     /protein_id="HEX9916749.1"
                     /translation="MIGIAGYGANMPRYRIKLEEIAKVWGADAPSYKKGLGLYEKSVP
                     APDQDTITMSVEATKNALKRAGIDPSEIGAVYVGSESHPYAVKPSGTVLAEAIGAVPD
                     CHCADFEFACKAGSEAMFVCLGLVKSEMTKYALAVGADTSQGAPGDALEYSASAGAAA
                     FIMGTENIIAECVETHSFMSDTPDFWRREHQFYPRHAGRFTGEPAYFKHILGAGKAIM
                     KKVGMKAEDFNYAVFHQPNGKFPMKVGQMLGFTKEQIEPGWLCPYLGNTYSGSSPMGL
                     TATLDIAKPQDKILMVSYGSGAGSDAFVWKVTERIEKVRDLAPKTRKMLESNKIYIEY
                     GTYAKFRGKIIRNE"
     gene            complement(10408..11508)
                     /gene="buk"
                     /locus_tag="VGB16_03350"
     CDS             complement(10408..11508)
                     /gene="buk"
                     /locus_tag="VGB16_03350"
                     /EC_number="2.7.2.7"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_013933089.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0047761 - butyrate kinase activity
                     [Evidence IEA];
                     GO_process: GO:0006113 - fermentation [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="butyrate kinase"
                     /protein_id="HEX9916750.1"
                     /translation="MANKNKHYTILVINLGSTSTKVSVFASEKEVLKKTIQHSKFELE
                     PFKRIWDQYEFRKKLIKGLLEDNNIKPDSLDAVVGRGGLFRPVVSGTYQVNEMMIEDG
                     RKAYGGEHASNLGCVLAFGIGWDFHIPAFVVDPPSVDELCKIARYSGLPEFPRKSLFH
                     ALNVKATARLAAKDLKKNLDEVNLVVAHLGGGVSVVALRKGKAIDSNDALCTGPFTPE
                     RSGTLPMFDFAEWCFSGKTTLDEIKRRMVGGGGLVAYLNTNDCQEVEDRIAKGDKEAE
                     SVYNAMIYEIACEIGKRAVTLKGKIDAIVITGGLAKSKVLVEKLKEWVGFLGKMLVYP
                     GEDEMEALALGALRVLRGEEKAKIYPERAEDE"
     gene            complement(11510..12586)
                     /locus_tag="VGB16_03355"
     CDS             complement(11510..12586)
                     /locus_tag="VGB16_03355"
                     /inference="COORDINATES: protein motif:HMM:NF012434.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016491 - oxidoreductase activity
                     [Evidence IEA];
                     GO_process: GO:0006520 - amino acid metabolic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="Glu/Leu/Phe/Val dehydrogenase dimerization
                     domain-containing protein"
                     /protein_id="HEX9916751.1"
                     /translation="MSLFELMEKHGHEQLIFCYHEPTNLKAIIAIHDTTLGSAIGGCR
                     VWKYRNEEDMINDALKLSKIMTYQSAISDCDTGGGKAVLWHDFEEEPKEAYFRTLGRF
                     IEGLNGGFITYPDLGTGTQEMKYIRRETKHVILYQSADKETAESSEITACGVYWGMKA
                     CVKEVFGISSLNGLVIAVQGVGSVGKRLVDYLKQEDVKLLVTDLNYDSIKSVQDKHPD
                     VKIIAPDEILKVQCDVFSPCALGPVITKQNIDQLKCRIIAGAAYDILEDWSLSQALSQ
                     KGIICAPDFVINTGEMFLTEDCLEILSKEKALDSTKKIYDIMAKVITKAKKENISLYQ
                     AAYLMAVERMKGVGEIKNILTRSK"
     gene            complement(12909..>13448)
                     /locus_tag="VGB16_03360"
     CDS             complement(12909..>13448)
                     /locus_tag="VGB16_03360"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEX9916752.1"
                     /translation="SVLILSIITLSIYFWVYLFKTLREMKRAFTFEASETNPDKVRRL
                     LIAYLIVFMVVEIVNEAIGWPFGTFVQPSKTTGFYVWLVIGNIIEAVFFVMFFSSFLK
                     LIELCQKQGGMAPLNKSALWALIGIDIGISFAAIGITSLTYLVPVILVDLVVSLIFLY
                     LIVKQVNRIWTESHKVRMS"
BASE COUNT         3433 a         3503 c         2487 g         4025 t
ORIGIN      
        1 atctcttttg aattcttcaa gtaacttaaa gctaatttag ctcctaagct ggaagaagaa
       61 tttagattct tgaagtaggt tgacaatttc tcatccttct tggggtccac caaaagtgct
      121 ttccaggcaa aaaatccccc aggatagtct cccaattttt tgtctagttc ctttgcccag
      181 gcattggagt caaacatctt gtattctcct ttttctatat cgtacactcc aactggtcgg
      241 ttcttctcgt attttaggaa tccatccttc tgtgttccat cacttctctg gccgcctaat
      301 actctcttct gcaccatcct ggccaaaagg tcatctttca aatcctcacc cttcaaatat
      361 tggctgaccg tctcagaaat gcaatgaaaa acatcaaccc ctacataatc tattaactgg
      421 aatatgccca taggacgaac cagcaagtcc tggctcactt tgttgacggc atatatcccg
      481 cctgccaagc cgagctccct ggataacctc tttgcttctc ttacaccata caaaccatct
      541 cttaagaaat gcccgtttcc aacgaagccg gcaatgtcat tcgaaggaac tatcttcttt
      601 ctcaatctct tggctaattc caaagccaaa tctttcagtt ccggtttggt ctcttttgca
      661 catatcagtt cagccaatct ctggactgct ggtggattat aaaagtgaaa tccgattatc
      721 cttccgttta agtttgcctc tttatttagt atatttatgg gaatggaaga ggtattggta
      781 agatagacga cctctttgcc acaaatctta tctaaatctt ttagcaaatt tactttcaaa
      841 tccttatctt ccaatactgc ctcgaaaacc atatttgagc ctttggccaa atttaaatca
      901 gtgctgcacc tcaccaggga ggcggcatcc tccacaaact gattgacgat gtcaaagttc
      961 tcaattaaat ccaggcggtc cttgtatagt tcccttaatg taactgcacc tttttcgcct
     1021 gcctttacca attgggtttt caagtacttc accaaaccat ccagagcgtc atcattcaca
     1081 tcaatcaaat tcaaacgata agcaaaatcc ttattctttg ggtttaattt ttctttagcc
     1141 atctggtcag ccagaagcaa agcgattccg cttcccatct ttcccgctgc cccgacaatc
     1201 gaaaccttcc ttatcttttc atccagattc atactatgcc acctccggat cttgtttttt
     1261 attattgttt aaaaactgta tttcattgcg aaactgtaac caccacttct tggtgttacc
     1321 ataaaatcaa gtctctgtgt attggcttgc tgttgcttca agctatcatt atatattcct
     1381 actccttttt tgagatgaga attagctgaa tggtatagca gttgtccagc aacaagagta
     1441 gctaacgtac ttataagcag aaaggtctga gtatcatctg tctcaaaact tctcctggcg
     1501 aaagccctac catatgaaac agccaacgga ggccatctta taaggtcaaa ccatatatat
     1561 aagcttcgtc tgcgacgata tttgtctatc tctacttgag cttctttgac gcctgccata
     1621 ttttgcttca accggagccc atctccaacc tgatgttgaa gctcatcttt cgtatatgaa
     1681 tatccccacc agaaactttt cttcagaaca atttcgccac cataacaagt agttgacaaa
     1741 ataatcagca agactatcaa cgctaacgaa gtcttgaaca taaagcctct ctctatcttg
     1801 aaatttattt cacaatctat taattcccac acctttgcag gtgtagttct tattgtttcc
     1861 ctacgggtcc tgttttgtca tccccgaggg tttaatcggg gatccatact tgcttttctc
     1921 ttctggattt cccgtatcga gtacgggaca agctctgctg aagttcatcc ctgcgaaagc
     1981 agggacagga atgacaattg aaaaattcct gttttagttg gttcgggaat tggaattccc
     2041 gaacctatcc tttgtgcgga aaatccaatt cccgcacaaa cggtagagtt tttgtttttt
     2101 gttatcttga gatttatttc acaacctttt tcaaaaaatt attctttagg ctccagctcc
     2161 acaattggct gacccgtatc aaccaaatca ccttctttga agtttatctt tttaacctta
     2221 gccggaatgg gtgattttat ctcgttttcc attttcattg cttcaactat cactaaggtc
     2281 tggttttttc ttacctcatc accaaccttt actgaaatct taactatctt gccgggcata
     2341 ggcgttgaga cgaaattctt ctcaccaaca tgcgtatgct ctccagcaaa gtttttcctc
     2401 ttcttctctt ctggcttctc tatgcaaaaa ctctctccat ttatatttac caagaatttg
     2461 ccatctctct ctgctaaatg ggcggttaac gcttgcccat ccattagaaa agaaatgcta
     2521 ttggaagaaa tgagttctgc atcaatatca tacctcttac catccagctc tattttatag
     2581 ctgttgcctt tagactctat tttaagcttg taagttttat cttgtatttt aaattcgtgt
     2641 tccatttaat ttctcatccc tatttcccat ttgcctaatg cctgccaagg ggaataactt
     2701 tcttgctcaa ctgcctgccc tttggatacg aatttatttt gcgacattat ggcagcagcg
     2761 attaaagctt gattcaaata tttcttgtta tcttcttttt ctttccaatc tgtgaaattc
     2821 cttggaataa aatcggtggt ggtcttgcct tcgaggaaat ccgaatgaga gaccacatct
     2881 tttaaaaacc ctgttgtggt ctttattccc aagacagtta aatctgataa ggcggttaac
     2941 atccttctgg cggcagttgg tctatcctct gcccagacga ctagtttgga aagaatcggg
     3001 tcgtaataaa cgcttacctc aaagccggta tagattccac aatcgtgcct gattcccgga
     3061 ccagttggct ctttcataaa cagaattttt ccggagcagg gcaggaaatt attctccggg
     3121 tcttcagcgt aaatcctgca ctcgatggca tgccctcttt gttttaattg gtcttgtttt
     3181 aaaaacattt tttcgccagc cgccaagcgg atttgggctt tgactaaatc tactccgacc
     3241 accagttcag ttatgggatg ctcaacttga atgcgggcgt tgacttcaag gaaatagaag
     3301 ttcttatctt cgtccaagag aaattcaact gttcctgcat tagaatagtt cgctgccata
     3361 atagccttga ccgcagtctc tcccattttc tttcgtaaag aatcatccaa ggcaaccgac
     3421 ggcgtttcct ccactatttt ttgatgacgc ctttgaatcg agcattcccg ctcaaacaga
     3481 tggactgcat ttccgaactt atctgccaaa acctgaaact caatatgtct cggatgctct
     3541 atatattttt ccagataaac tgaatcgtct ccaaaagcgc tccttgcctc tcgcattcca
     3601 gcggaaatag atgattccaa ttcatcttca gaacgaacct ttctcattcc ttttccccca
     3661 ccacccccgg atgctttgag tatgaccgga tagccgattt gttttgcctc ttttttgaat
     3721 tcatttatat cttttgaact tcctttcatt ccgggaatga ttggtatgcc ggctctctgc
     3781 atagtctctc tggattgaat tttatctcca accaaagcca atgattttga atccgagccg
     3841 atgaaaacta ttccttcttt ttcacaacgt tcggtaaaag caggattttc agccaaaaag
     3901 ccatatcccg gatgaatggc atccgctttc aatttctttg ctatggagat aatgctatcg
     3961 atattgagat aactttccaa cggagctgga ggaccaacca aaaccgcttc atctgccatc
     4021 tgaacgtgaa gagaatttct atcaactgtg gagaatatgg caaccgtggg aatgccaagc
     4081 tccttgcatg ccttgatgac ccttactgcg atttcaccac gattggcgat taagattttg
     4141 ttgaacataa tagaaactag actcctgaac tctcaaactg ccttttatgt ggctgtcagg
     4201 tcccctgacc tgacagcgga tttcctgtgt aggggcgtat tgcaatacgc ccctacattt
     4261 caccatacaa atacgaatta tcgttatgtc aggacccagt cttgacatct tttctatgtt
     4321 tcgcagccgt caggagtagc ttctgacgga acaataagac tggattcccg ctttcgcggg
     4381 aatgacaaac gattattagt ccttgctcca tttcggtttt cgtttttcca aaaaagcatt
     4441 catcccctct tgcccctcat cactgattcg aagtttagct atgacctcgg cggtgtaact
     4501 ggtggctaaa tccaagttca tcaaaggtac tttttctaag agttctttac aacacttcaa
     4561 cgcctctggg ccactggaga ttagctgctt gactttttca tcaactgctt tgtccaaatc
     4621 gtttgaaggg acgacttcat ttaccagccc aaattttgat gctctttctg cactcaacct
     4681 ttccccggtc aaaaagaatt ccctacacct actttccccc attcgacgaa ttacataagg
     4741 tgagatacag gcaggaacca agcccaactt gacttcgctt aaggagaact ttgcttcctc
     4801 tgaagctatc acaatatcgc agactgaaac caaacccgtc cctcccccaa tggctgcacc
     4861 attgaccttg gcaatagtcg gcttaggcaa agaatagatt aaataaaaaa gctgcgctaa
     4921 cttaaatgag tcatcgaaat tctcttcgta ggaaaaactt atcacttttt tcatccagtt
     4981 taggtcagcc ccagcgcaga aagatttacc cttaccagtc aagaccacca cgcgaatatg
     5041 ttcatctttg gagattttct caaaaacctc ggttagctcc tttatcatca cctcattgaa
     5101 ggcattatgt acttccgggc gatttaaaat tacattagca accgcatcct cggttttgaa
     5161 aattatggtc tgataatgtt ccaattttct ccccttcact cgcctccgcc agaaagtctc
     5221 gctcgcctct cggcgctcgc ctctcggcga agccgaggga agccgagggc ctttggcgga
     5281 aatagcgagg cgagcctcgc cgaagtcccg cagagccgga cgaaggcggg cgaagtttcg
     5341 cttttatatt tgggaagctc ccgtaggggg gcttcacatt ctgaatatgc catacctttg
     5401 gtcgggaatg gtggcgttca aagccatcga gattcccaaa cccaagactg ctcttgtgtc
     5461 caaagggtcg attatcccat catcccacaa atgcgcagtg ctgaagtagg gacttgattc
     5521 ggtctcatat ttttccagag tgggagccat aaatttctcc tcctcctcct tggacataac
     5581 tatccccttt ttctccaact gtcttttctt taccgttaag agaacattag ccgcctgctc
     5641 accacccatc acgcatattt tggcattggg ccacatccaa agaagtcgag ggtcataagc
     5701 tcggccgcac attccataat tcccagcacc gtatgagcca ccaactatca ctgtgaattt
     5761 cggaacatcc gcatttgcca ccgcatgcac catcttcgcc ccatctttgg caatgccgcc
     5821 atgttcatat tgttttccca caatgaagcc ggtgatattc tgcaaaaata tcaagggaat
     5881 tttacgcata gtgcaaagtt cgataaagtg ggcagcttta agtgaactct ctgaaaacag
     5941 aactccgttg tttcccagaa ttccgaccgg ataacccatt attcgtgcaa atccacaaat
     6001 tattgtggta gcgtacaact ccttgaattc ctgaaattcg ctcttgtcaa ctatacgggc
     6061 tatgatttcg cgaatatcat agggttttct tatgtctcga gggacaatcc cgaaaatctc
     6121 cttagggtca taataaggct cctctgcttc agaggtatgt aatcggaatt tctcaggtgg
     6181 attaagattc tcaactatat ttcgggtgat ctccaaagcg tgctcatcgt tataagcata
     6241 atggtctgcc acaccagaaa ttcggcaatg cacatccgca ccacccaact cctcatctgt
     6301 tacctcctcg ccagtggcgg ctttaaccag aggcggccca ccgataaata tcgttccctg
     6361 ttttcgaact atcactgcct catcgctcat cgctggaaca taagccccac ctgcagtgca
     6421 ggagccgagc accactgcaa tttgtggaat gcccatagcc gacattcgag cttggttata
     6481 aaatattttt ccgaaatggt ctttgtccgg aaaggtgcca gattgatagg gcaagaatat
     6541 tccgccggag tcaaccagat agatacatgg cagacgattc tctgtggcta tctcctgggc
     6601 gcgcacatgt tttttgatag tcaagggata ataagttccc cctttgactg tagcatcgtt
     6661 cgacaccacc aacacttcgc gaccgtgcac tactccaatc ccggtgatca ccccggctga
     6721 aggggcatca ttatcgtaca tatcataggc tgccaaagcg ctcaattcca agaagggact
     6781 gtttttatca aagagcaggt ccaatctttc acgggcggtc aacttacctc tttctttatg
     6841 acgcttcttc atatcagctg gaccaccctc tttgactttg gctaacctct ccttgaactg
     6901 gtcaaccacc ttctgcatag ctttctggtt ttccttgaat tctatcgctt gagtatcaac
     6961 cttggattct attctaaaca tattctcctc tgctcaaaat gctctgttca aattcgcgta
     7021 ggggtggagt tcatctccac ccgcacccgt tcgggcgaag tcaagcttcg cccctacgtg
     7081 ggctcctaaa tcggaacggc tttgtagcca taacagatta ttcctgcttt tccgtcctcc
     7141 tgaagtttgc gaaattcaat cttgagcttt attccagtcg agagtttatc taagtcgcaa
     7201 tccgttatct gggctaatat tttaactcct tcttccaatt ccaccacgcc caaagcgtac
     7261 ggtatctggt cgatgaattg ggatggcccc acgcgaatta tggtgtaggt caccagcttt
     7321 cccgtatctt tcaatttgta tttttcgaat cctttggatt tacaggcgga acaaattagt
     7381 ctcggcggaa aatatatttt cccacaactt ttgcatttct ctgcctccag acgataacgt
     7441 tgaggcatct ctctccaata tcttgcggat aacattatgc cacctccagg atatgaacca
     7501 ctgtgcttcc accgcttcct cccatattct gagcaagtcc caccttagca ttcttgactt
     7561 gccttttccc cgcttctcct cgaagctgtt tggtgatctc aactatttgt gccactccgg
     7621 ttgcaccaac tggatgaccc ttcgatttca ggcctccact ggtgtttatg ggaattttac
     7681 ctccgagggt ggtcatccca ttctcagttg ctttgcccgc ctcacctttt tccgtcaaac
     7741 ccaaagactc gagcacgatc aactccgcaa tggtgaagca atcgtgaact tcagccaagt
     7801 gaatgtccga gggtttctta ttcgccatct tgaaagctac ttcccctgct ttctgagttg
     7861 ctttaaggaa agttaaatca tctctcgaat gcaaggcgat tgtgtctgtg gcttgagctg
     7921 agccaattat cttaaccaac ggctttttca aaccgagctc cttagctttt tcgagcgaac
     7981 agaggataat tgcagccgca ccgtcagtta taggagagca gtccaaaaca gtcagagggt
     8041 cagccaccat taccgaattt atcacctgct ccactgtaat tttcatagga aactgtgcca
     8101 gaggattcat tgagccgtgc ttgtgattct tgactgctac ttgggctaac tgctcgcgtg
     8161 tggttccata tttttccata tgcgcacgag ccatcatggc gtaaagcccg ggaaaggtta
     8221 ccccttggta aacttcgtac tcctggtcag ctgcagttgc caaggcgaaa gttgcaccat
     8281 ccacgctgac atcggtcatc ttttcgactc ctcctgctaa gacgatatcg cttatgcctg
     8341 aggcaacttc aataaatccc gctctaactg ctgcacctcc ggaggcacag gcggattcca
     8401 cgcgagtaca gggaattggg ttcactccca aatagtctgc taaaagcgaa cccaaatgtt
     8461 cttggtggac gaaaagccca ctggacatac agccgacata catcgaatcg attttatcca
     8521 cccctgcatc gtccatcgcc tctaaagcag cttccacgaa gatatccctt aaggatttat
     8581 cccataactc accccatttg ttcatcccca atccaattac acaaacctct ctcatattct
     8641 ctcctattcg tttcttatta tcttccctct aaacttagcg taagttccat attcgatata
     8701 gatcttgttg ctttctaaca tctttctggt ttttggcgct aaatccctga ctttttctat
     8761 tctttctgtt actttccaca caaaagcatc gctacctgct cctgagccat aggagaccat
     8821 taaaatttta tcttgtggct tagctatatc caaagttgcg gtcagtccca taggagatga
     8881 gccggaatag gtgttcccta aatacgggca gagccaaccc ggctctatct gctccttagt
     8941 gaaacccagc atctggccaa ccttcatcgg aaattttcca ttgggctgat ggaaaaccgc
     9001 gtaattgaaa tcctccgctt tcattccgac ttttttcatt atggctttgc ccgctccgag
     9061 gatgtgctta aaatatgccg gctcaccggt gaaacggcca gcgtggcgag gataaaactg
     9121 atgttccctt ctccagaagt caggggtgtc gctcataaag gaatgagttt cgacgcattc
     9181 tgcgattatg ttttcggtcc ccattataaa tgcggctgcc ccggctgaag ctgaatattc
     9241 taaagcatcg ccaggtgcac cttgcgaggt atcagctcca actgccaaag cgtacttggt
     9301 catttcggat ttaactaaac ccaaacagac gaacatcgcc tctgacccgg ccttgcaggc
     9361 aaactcgaaa tccgcacaat gacaatccgg aacagccccg atggcttctg ctaaaacagt
     9421 tccgctgggt ttaactgcat agggatgaga ttcggaaccg acatacactg ctccgatttc
     9481 acttgggtct attcctgctc tcttcaaagc attcttagtt gcctcaactg acatggttat
     9541 ggtatcctgg tctggagcag gaacgctctt ttcataaaga cccaatccct tcttgtaaga
     9601 tggagcatcc gcaccccaga ctttggctat ctcttctaat ttaatacgat aacgcggcat
     9661 attcgcaccg taacccgcaa tgccaatcat aaaacctcac tttgaattaa agttgatttc
     9721 aattccttct actacatagt agactacaaa gtgccaaact tctaacctgt cttgtcgggt
     9781 agctctgggt ctcctgaccc agagtggaca aggtcaggag gccttgtcct acccgtgaac
     9841 tcttaaactg ggtaagtaat ggctgcgcca ttactttttc taactgggaa cggtgcaacc
     9901 gttccctacc cactgaaata tcgcgtatcc ccaccttgcc ttcaaggtgc gacctaaccg
     9961 acccacgttg agggcaacgt gggcttactg ttcgctcgat gaatcgagca actacaaatt
    10021 ctatctctgt actttcctac aatgaatgac caactgaccc aacgtagggg ctcgatttat
    10081 ggttcgactt tgctcaccat cctgagtttc tcgaagggtc gagcccgatg ctggcgggtc
    10141 ggatgtggtt cagcaagggc ttcgactgag ctcagccgaa cgtcttcacc atctcttcga
    10201 gcctgagcct cagagccgaa ggcctgagca aggtcgaagg aaatccgacc cctacatttt
    10261 ttcttcgatt ctaaccttat cgtctagttt tgatttaagc tgattaatca agtcctcgta
    10321 gtcacgtatt cccaccttac cttcaaggtg ggacccacca cgtccacgtt gcctgcccgg
    10381 ccagggaggg caacgtgggt ttactggcta ctcatcctcc gctctttctg gatagatctt
    10441 ggctttctcc tctccacgta atacgcgcaa agcccctaag gcaagagcct ccatttcatc
    10501 ttcgcccgga tataccaaca ttttccccaa aaaaccaacc cattccttca gtttttcaac
    10561 caacaccttc gacttagcta aaccaccggt tatgacgata gcatctatct ttcctttcaa
    10621 ggttactgcc cttttcccga tctcacaggc gatttcgtat atcatcgcat tatagacgct
    10681 ctctgcctct ttatccccct tggcgattct atcttcaacc tcttggcaat cattagtatt
    10741 aagataagcc actaaacctc ctccacccac cattcttctt tttatctcat ccagagtagt
    10801 ttttccagaa aaacaccatt cagcgaagtc gaacataggc aatgtgcctg acctttctgg
    10861 agtgaaagga cctgtgcata aggcatcatt ggaatcaatt gctttgccct ttctcaatgc
    10921 cacaaccgat acacctcccc ccagatgagc aaccaccaga ttcacctcat ccaaattctt
    10981 tttcaaatct ttggcagcca gtcgtgcagt ggctttgacg tttaaagcat gaaaaagact
    11041 ttttctggga aattccggca ggcccgaata gcgcgctatt ttgcataatt catccactga
    11101 agggggatca accacaaaag ccggaatatg aaaatcccat ccaattccaa aagctagaac
    11161 gcaacctaaa ttcgaggcgt gctctcctcc gtaagccttg cgaccgtcct cgatcatcat
    11221 ctcattcact tgataagttc cacttaccac tggtcgaaaa agaccacccc ttcccacaac
    11281 cgcatccaac gaatccggct tgatgttatt gtcttccaaa agccctttga ttaacttctt
    11341 tctgaactca tactggtccc aaatcctttt aaatggctca agttcaaatt tgctatgctg
    11401 tatggtcttt ttcaatacct ccttctcgct ggcaaaaacc gaaactttgg tggaggtcga
    11461 accaaggtta atcaccaaaa tagtataatg cttatttttg ttagccattt cacttggaac
    11521 gggttaaaat attcttaatc tctcccacgc ccttcatcct ctccactgcc atcaaataag
    11581 ccgcttggta caaagatata ttttcctttt ttgccttagt gatgacctta gccattatgt
    11641 cataaatttt cttggttgag tccagtgctt tctctttgga taaaatttcc aggcaatcct
    11701 cagttaaaaa catctctccg gtatttatca caaagtccgg tgcacagatg atgccctttt
    11761 gggaaagagc ttggctcaaa ctccagtcct ccaggatatc gtaagctgca ccagcgatta
    11821 tcctgcactt cagttggtca atattctgtt tggttattac cggacccaaa gcgcaagggg
    11881 agaaaacatc acactgaact ttcaagattt catctggagc aatgatcttc acatctggat
    11941 gtttgtcctg aactgacttg atggaatcat agttcaaatc ggttaccagt agtttcacgt
    12001 cttcctgctt aagatagtcc accaaccttt tccctacgct tcccacccct tgaaccgcta
    12061 tcactagccc atttagagac gaaatgccaa atacttcctt gacgcaagcc ttcatacccc
    12121 aatacacgcc acaggcagtt atctctgagc tttccgcagt ctccttatct gcggattgat
    12181 agagaatgac atgttttgtt tcccggcgaa tgtatttcat ctcctgcgtg ccggttccca
    12241 agtcgggata agtgataaat cctccgttta aaccttcgat gaaacgaccc agagttctaa
    12301 aataggcttc ttttggttct tcctcaaaat catgccagag caccgcctta ccaccaccag
    12361 tatcgcaatc ggaaatagcc gactgatagg tcattatctt tgaaagcttc agggcatcgt
    12421 ttatcatatc ctcttcgttt ctgtatttcc acacccgaca accgccgatc gcacttccca
    12481 aggtggtgtc gtgaatagca attatggctt tcaaattggt tggctcgtgg tagcagaaaa
    12541 tcagttgttc atgtccatgt ttttccatca actcaaaaag gctcataaat tattccttct
    12601 taaattctta tactcattat gtatccccac cttgccttca aggtgggatc caacataccc
    12661 actttgaggg caaagtgggt ttaccgtttc aggataggtt ttagtctctt gaacccttcc
    12721 ctcatccctt ctttcattat caacttacct tttcctctga agtaatcctt tttcacctgt
    12781 cattctgagt ccgccttcgg cggaagaaga atctcagaga ctcttcgctt cgctcagggt
    12841 gacaaagtcc ttccccgcat gacaaaggat ggatatacct gacctacgaa aatagttaat
    12901 aagcaacatc aagacattct aactttgtgt gattccgtcc agatcctgtt aacttgtttt
    12961 actatcaggt aaagaaagat caaactgaca actagatcga ctaatattac cggaacgaga
    13021 tatgttaggg atgtaatgcc gatagcggca aaagagattc ctatatctat tccaattagt
    13081 gcccagagag cagatttgtt tagaggagcc attcctcctt gcttttgaca taattcaatc
    13141 aactttagaa aagatgagaa aaacataaca aaaaatacgg cctcaatgat gttaccaatg
    13201 accagccaga catagaatcc tgtggtcttt gagggttgaa caaaagtccc aaaaggccag
    13261 ccaattgcct cattaacaat ctcaactacc atgaacacta ttaagtacgc gatcaagagc
    13321 cgcctaactt tatctgggtt tgtttcgctg gcttcaaatg tgaaagccct tttcatttcc
    13381 cgaagagttt tgaatagata gacccagaaa tatatcgaga gcgttatgat agagagtata
    13441 agcacaga
//
LOCUS       DASWLN010000100         1987 bp    DNA     linear   ENV 20-FEB-2024
DEFINITION  MAG TPA_asm: Candidatus Zixiibacteriota bacterium isolate
            SMAG_U2949 k141_1059803, whole genome shotgun sequence.
ACCESSION   DASWLN010000100 DASWLN010000000
VERSION     DASWLN010000100.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37960594
            Sequence Read Archive: SRR10376091
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Candidatus Zixiibacteriota bacterium (soil metagenome)
  ORGANISM  Candidatus Zixiibacteriota bacterium
            Bacteria.
REFERENCE   1  (bases 1 to 1987)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 1987)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (26-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/12/2024 08:28:26
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 1,440
            CDSs (total)                      :: 1,416
            Genes (coding)                    :: 1,412
            CDSs (with protein)               :: 1,412
            Genes (RNA)                       :: 24
            rRNAs                             :: 1 (16S)
            partial rRNAs                     :: 1 (16S)
            tRNAs                             :: 21
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 4
            CDSs (without protein)            :: 4
            Pseudo Genes (ambiguous residues) :: 0 of 4
            Pseudo Genes (frameshifted)       :: 0 of 4
            Pseudo Genes (incomplete)         :: 3 of 4
            Pseudo Genes (internal stop)      :: 1 of 4
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..1987
                     /organism="Candidatus Zixiibacteriota bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_1059803"
                     /isolate="SMAG_U2949"
                     /isolation_source="soil"
                     /db_xref="taxon:2053527"
                     /environmental_sample
                     /country="USA: Oregon"
                     /lat_lon="42.99 N 123.63 W"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            <1..526
                     /locus_tag="VGB16_03365"
     CDS             <1..526
                     /locus_tag="VGB16_03365"
                     /inference="COORDINATES: protein motif:HMM:NF018798.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=2
                     /transl_table=11
                     /product="DUF1385 domain-containing protein"
                     /protein_id="HEX9916753.1"
                     /translation="LTGLIRVTLFLGYLWGISLFGEFKKIFQYHGAEHKTIYAYENGE
                     SLDLENVRKHSTQHPRCGTSFIFLVLVFAILFYSLSDSIYSLVAGSSLSLAKRMLFHF
                     MLLPLVAGGVYELLRLSDLKSDNRIIKILNAPGLWLQKITTQEPNQAQLEVGIRALED
                     AIAQKGDLNVGDIK"
     gene            507..1595
                     /gene="prfA"
                     /locus_tag="VGB16_03370"
     CDS             507..1595
                     /gene="prfA"
                     /locus_tag="VGB16_03370"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_013779159.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016149 - translation release factor
                     activity, codon specific [Evidence IEA];
                     GO_process: GO:0006415 - translational termination
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="peptide chain release factor 1"
                     /protein_id="HEX9916754.1"
                     /translation="MLEILNKIKEQYQKLTRELSNPQVFSDQKRFKKLSKEHAHLTQI
                     MELSKEYENILKLMGEAEEVKNSSTDKELLELAKEELVELYPKKEKLEDDLKKMLIPK
                     DPQDDKSGIVEIRAGTGGEEAALFAADLYRMYVKYAEKKGWKVETLSSNPTGVGGFKE
                     VIFSVEGQGAYGNLKYESGVHRVQRVPKTEASGRIHTSASSVAVLPEAEEVDIEIKPE
                     DLRIDVFRSSGPGGQSVNTTDSAVRVTHLPTNTVVTCQDEKSQLKNKTKALKVLRARL
                     LDKALTDQKQKVDQERKSMVGTGDRSAKIRTYNFPQGRVTDHRIGLTLYRLEDVLAGN
                     LDEFIQAFKQKEQEQKLSLKEKVNQNEA"
     gene            1585..>1987
                     /locus_tag="VGB16_03375"
     CDS             1585..>1987
                     /locus_tag="VGB16_03375"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEX9916755.1"
                     /translation="MKLRIILSLILALVVMFYGRQLSLRRSSLLVIQSGDVRIEHLTV
                     VNKIGPGDVKIEAKITTPQPYADYTKKLFYKIDDGAYKPVDFVPLESDSTSKFFAKIP
                     AQEKGKRAYYYMMVEDKQGNKVALPDKIETID"
BASE COUNT          616 a          406 c          491 g          474 t
ORIGIN      
        1 cttaaccggg ctgatacgag tgactctatt tttgggatac ctttggggga tatctctgtt
       61 tggagaattc aagaaaatct tccaatatca tggggcggag cataagacca tctatgccta
      121 tgaaaatggg gaaagcttgg atttggaaaa cgtgagaaag cacagcaccc agcatcctcg
      181 ctgtggaacc agtttcatct tcctggtctt agtcttcgcc atactgttct attcgctatc
      241 agattcgatc tattctcttg tggccggctc ctccctttct ctggctaagc gaatgctttt
      301 ccattttatg cttttgcccc tggtggctgg aggggtgtat gaattgctca ggctctctga
      361 cctaaaaagc gacaaccgta taatcaagat tctcaatgcg ccaggtctgt ggttgcagaa
      421 gataaccact caagagccaa atcaggcgca actcgaagtg ggcatccgtg ccttggaaga
      481 cgctatcgcc caaaaaggtg atttgaatgt tggagatatt aaataagatt aaagagcaat
      541 atcagaaact cacccgagag ctttccaatc cgcaagtctt ctctgaccaa aagagattca
      601 aaaagctctc caaagagcat gcccatctca cgcagataat ggagttgtcc aaggagtatg
      661 agaatattct aaagcttatg ggcgaagctg aagaagtaaa gaatagctca actgacaagg
      721 aactgcttga gctagccaaa gaggaattgg tagagcttta tcctaagaag gaaaagctgg
      781 aggatgactt aaagaagatg ctcatcccta aggacccaca agatgataaa agcggtatag
      841 tggagataag ggctgggact ggcggtgagg aagcagccct ctttgcagct gatctatata
      901 ggatgtatgt caagtatgcg gaaaagaagg gatggaaagt ggagaccttg agctccaatc
      961 caaccggagt gggtggattc aaagaggtta tattctcagt tgagggccag ggggcttacg
     1021 gtaatttgaa atatgaaagt ggagttcatc gggtacaaag ggtgcctaaa acagaagcta
     1081 gcggtagaat tcacacttca gcctcatccg tagcagtcct accggaagct gaagaggtgg
     1141 atatcgaaat caaaccagag gacttgagga ttgacgtttt cagatcttcg ggtccgggcg
     1201 gccaatcagt taacaccact gactccgctg tcagggtcac ccacctcccg actaatactg
     1261 tggtcacctg tcaggatgag aaatcgcaac taaagaacaa aaccaaagcc ctgaaggttc
     1321 taagggcgag attgctggat aaggctttaa ctgaccagaa gcaaaaagtt gaccaagaaa
     1381 gaaaaagtat ggttggaacc ggagaccgct ctgccaagat aagaacttac aactttcccc
     1441 agggaagggt tactgaccac agaatcggct tgactttgta ccggctggag gatgttttag
     1501 caggaaacct ggatgaattc atacaagcct tcaaacagaa ggagcaagaa caaaagttgt
     1561 ctttgaagga aaaggtgaat caaaatgaag cttaggataa tcctgtcctt aatcttggct
     1621 ttagttgtaa tgttttatgg tcgccagctc tccttgagga gaagctccct cttggtgata
     1681 cagagcgggg atgtgcgcat tgaacaccta acagtggtta acaagattgg tcccggagat
     1741 gtaaaaatag aagcaaagat aaccactccc caaccttatg cagactacac caaaaagtta
     1801 ttctacaaaa tagatgatgg agcttataag ccagttgatt ttgtgccact ggaaagcgac
     1861 tcaacttcga agttctttgc caaaatacct gctcaagaaa agggtaaaag ggcatattat
     1921 tatatgatgg tagaggacaa acaggggaac aaggttgctc ttccagataa gattgaaacc
     1981 atcgacc
//
LOCUS       DASWLN010000101         4118 bp    DNA     linear   ENV 20-FEB-2024
DEFINITION  MAG TPA_asm: Candidatus Zixiibacteriota bacterium isolate
            SMAG_U2949 k141_802879, whole genome shotgun sequence.
ACCESSION   DASWLN010000101 DASWLN010000000
VERSION     DASWLN010000101.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37960594
            Sequence Read Archive: SRR10376091
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Candidatus Zixiibacteriota bacterium (soil metagenome)
  ORGANISM  Candidatus Zixiibacteriota bacterium
            Bacteria.
REFERENCE   1  (bases 1 to 4118)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 4118)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (26-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/12/2024 08:28:26
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 1,440
            CDSs (total)                      :: 1,416
            Genes (coding)                    :: 1,412
            CDSs (with protein)               :: 1,412
            Genes (RNA)                       :: 24
            rRNAs                             :: 1 (16S)
            partial rRNAs                     :: 1 (16S)
            tRNAs                             :: 21
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 4
            CDSs (without protein)            :: 4
            Pseudo Genes (ambiguous residues) :: 0 of 4
            Pseudo Genes (frameshifted)       :: 0 of 4
            Pseudo Genes (incomplete)         :: 3 of 4
            Pseudo Genes (internal stop)      :: 1 of 4
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..4118
                     /organism="Candidatus Zixiibacteriota bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_802879"
                     /isolate="SMAG_U2949"
                     /isolation_source="soil"
                     /db_xref="taxon:2053527"
                     /environmental_sample
                     /country="USA: Oregon"
                     /lat_lon="42.99 N 123.63 W"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            <1..659
                     /locus_tag="VGB16_03380"
     CDS             <1..659
                     /locus_tag="VGB16_03380"
                     /inference="COORDINATES: protein motif:HMM:NF012271.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005515 - protein binding [Evidence IEA]"
                     /codon_start=3
                     /transl_table=11
                     /product="fibronectin type III domain-containing protein"
                     /protein_id="HEX9916756.1"
                     /translation="GHFTDEAGNIAPTATAPGKVTIRIPPDPVVLLKPSGQTQSSLTL
                     YWSPSTASDFKYYQLYRAKAPSVDTNSTSITIITSKTTTTYTDSNLEDTTTYYYRVYV
                     YDNTGLSTASNTESGTTLKNQPPEPVVLSITSINHDSSLVLSWTENRDEDFESYRIYK
                     SQAPDTVTISSTLIKIINQNSTTSFTDEDVLPSSHYYYKVFVFDKGGLYTPSGQVYGP
                     "
     gene            673..1509
                     /locus_tag="VGB16_03385"
     CDS             673..1509
                     /locus_tag="VGB16_03385"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEX9916757.1"
                     /translation="MRLLDKVNNFIFTYLETFRSFKRFSLWVPFLIYGLFQTIILLIL
                     VSFPSGVVAGFFLPLLNKWYGEAILHYPTFFILLPALFNRTNLILGLIVGVLLDGTAV
                     FMFCSHFTQARISFFSGIKLALSKYWMLFVLGIFRLLVIWVMKVPFWLFKGMVVGSPK
                     REFALEMGCLALGVIYTGFFIYATPAVIWHRKKLGQAIRDSWNIYIHNFFSTFLFIFI
                     PLVIEFPINFIERQGPTVITKFNPEVVTWILILGIIVSVFANFILSGTVVKFYLGEET
                     TE"
     gene            1547..3640
                     /gene="prsK"
                     /locus_tag="VGB16_03390"
     CDS             1547..3640
                     /gene="prsK"
                     /locus_tag="VGB16_03390"
                     /EC_number="2.7.13.3"
                     /inference="COORDINATES: protein motif:HMM:TIGR02916.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0000155 - phosphorelay sensor kinase
                     activity [Evidence IEA];
                     GO_function: GO:0004673 - protein histidine kinase
                     activity [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="XrtA/PEP-CTERM system histidine kinase PrsK"
                     /protein_id="HEX9916758.1"
                     /translation="MVANSAISLVSTVVMLGTAVWVLAKNSRASVSRLFCLSLFCLIL
                     IQIGFFVASAFESITQINWGYYLIVFGLCFLPFSWIWFTLVFARESYEITLKRWKYAL
                     LAVFVLGIGFLAFSINKELIFLDKNIFGAMHFYFSRGGILFLIFLLISSVLVIINLEN
                     TFRFSGNNLRKQLLVPVSFITLLALATIFSVSHAMLLARIGTYPFVLVGIISVFMCIS
                     VGRYIVKSDLSIGTVYIGRRAVYSSIALILIGAYLLFIGIVAKIVQMIGGNLNIFYSI
                     IAAFLVIVIFSVLVVSSSVKQRIKSFVDKTFYRGRFDYQKEWSELSESISAILDLDEL
                     FSRISNSLSQNLETRGIQFFLAQKDGTFLLSYPKAERAQAEIEKGDEFLEWVWLYGKP
                     IRVEELEQQSKSLNSSFLKGGYLNSINTRVLVPLITKRKLIGVLLLGEKKDKKPYTNE
                     ELVLLEAVAHQLAIAILNAKMSEELLLSKEMESFHKLSSFVVHDLKNAISMLSMLLQN
                     AKDNMHDPNFQKGALLTVSDAVERMRGLVTKISSPSKEMQLDISECNLSELIKKVVTG
                     IDPLNFPRIKFNLDLNDTAMARLDESQISKVLQNLIINAVESMPPQGGVIDISVYVKD
                     YFWCVAVSDSGVGMSREFMKDKLFKPFQSTKKKGLGIGLYQSKEIVEAHGGKIEVESA
                     EGKGATFEIRLPIDK"
     gene            3854..>4118
                     /locus_tag="VGB16_03395"
     CDS             3854..>4118
                     /locus_tag="VGB16_03395"
                     /inference="COORDINATES: protein motif:HMM:NF012301.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_process: GO:0000160 - phosphorelay signal transduction
                     system [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="response regulator"
                     /protein_id="HEX9916759.1"
                     /translation="MKDKILIVDDDEGIRTQLNWALSGEYDVFLAENANQALEIAKKE
                     KPDLVALDITLSPLEEEKSGLDIIESFLEIDPLVKIIMITGHDE"
BASE COUNT         1133 a          820 c          921 g         1244 t
ORIGIN      
        1 caggacactt cacggatgag gcgggcaata tcgcaccgac tgcaaccgcc ccgggtaaag
       61 ttacaatccg cattccgcct gacccagtag tcctgcttaa accgtctggg caaacacaga
      121 gcagtttgac cttgtattgg agcccatcaa cagcaagcga ttttaaatat taccagcttt
      181 atcgcgcaaa agctccttca gttgacacga attcaacatc aataactatt atcaccagca
      241 aaaccaccac tacctacacc gattctaatt tagaagatac cactacttac tattaccgcg
      301 tttatgtgta tgataatact ggactttcca ccgcaagtaa cacagagagc ggaacaactt
      361 tgaaaaatca accacccgaa ccagtggttc tatccattac ttcaataaat catgactcca
      421 gcctggtctt gagctggacc gagaataggg atgaggattt tgagtcatat cgaatctata
      481 agtcgcaggc cccggataca gtaacgataa gttcaacttt aatcaagata ataaatcaga
      541 attcaaccac ctcttttacg gatgaggatg tcctgccaag ttcgcattac tattacaagg
      601 ttttcgtttt tgataaaggt gggttgtata ctccatccgg acaagtttac ggcccttgaa
      661 ggagactgaa aaatgagact acttgataag gtaaataatt ttatcttcac ctatctggag
      721 accttcaggt cgttcaagag attttcctta tgggttccgt tcttgatata cggcctattc
      781 cagacaatta tccttttaat tttggtctct tttccctccg gggttgtggc agggtttttc
      841 cttccccttc taaataagtg gtacggggaa gcaatcttgc actatcccac cttttttatt
      901 ttattgccag ctttgttcaa ccgaacgaat ctgattctgg gtttaattgt tggtgtgctc
      961 ttggatggaa cagcagtctt tatgttctgt tcacatttca cacaagcccg tatttctttt
     1021 ttttcaggga taaaacttgc cctctcaaaa tactggatgc tttttgtttt gggcatattc
     1081 agattgttgg tcatctgggt gatgaaagtt cctttttggc tatttaaggg tatggtagtg
     1141 ggaagtccca aaagggaatt tgctctggag atgggatgtt tggctttggg tgtgatatat
     1201 accggttttt tcatttacgc taccccggca gtgatatggc atagaaaaaa acttggccag
     1261 gcaatcaggg atagttggaa catatacatt cacaatttct tcagtacatt tcttttcatc
     1321 ttcattccct tggtaattga atttccgatt aacttcatag agagacaggg tccaacagtg
     1381 ataaccaagt tcaatccaga ggttgttacc tggatattga ttttaggcat tatcgtctca
     1441 gtttttgcca atttcattct gtcagggaca gtcgtcaagt tctatctcgg ggaagagacg
     1501 acggaataat cttgtgagtt ggagcaggtg gtgaagaggg caaaaaatgg tagcaaattc
     1561 agctatttct cttgtctcaa cagtggtgat gttaggcacc gctgtctggg tgttagccaa
     1621 aaattcaaga gcttcggtca gtcgactttt ctgtttatct ttattctgcc tcatcttgat
     1681 tcaaatcgga ttttttgttg cttcggcttt tgaatccata acccagataa actggggata
     1741 ttacctcata gttttcggtt tgtgtttctt gccattcagt tggatttggt tcactttagt
     1801 ttttgcacgg gaaagctatg aaatcacctt gaagcgatgg aaatacgctc tgttagcggt
     1861 ttttgtttta ggtataggtt ttctggcttt ctccatcaac aaagaattaa tctttttgga
     1921 taagaatatc ttcggagcta tgcacttcta cttctcccgg ggcggtattc ttttcctcat
     1981 cttcctgctt atctccagcg tactcgtcat tatcaatctg gagaacactt tcaggttctc
     2041 gggaaataac ctgcgaaagc aactcctcgt gccggtgtca ttcataacct tgcttgcact
     2101 cgcaactatt ttttctgtca gtcacgccat gcttcttgct agaatcggca cctatccctt
     2161 tgttttggta ggcatcatca gtgtctttat gtgcatatca gtgggaagat atatcgttaa
     2221 atccgattta tccatcggca cagtctatat tggccgcagg gcagtctact cctcaatcgc
     2281 tttgattcta attggcgcat acttgctctt tattggtatt gtggctaaaa tagttcaaat
     2341 gattggcgga aacctgaaca ttttttattc catcatagcc gccttcctgg tgatagtcat
     2401 cttttcagtt ctggtggtgt caagttcagt gaagcagagg ataaagagtt ttgtggacaa
     2461 gacattctat cggggcagat tcgactacca aaaggagtgg tcggagctaa gcgagagcat
     2521 atcggccata ttggatttgg atgaactgtt ctccagaata agcaattctc tttcacagaa
     2581 tttggagaca agaggcattc agttcttctt ggctcaaaaa gatggcactt ttcttttgtc
     2641 ttatcccaaa gccgaaagag cacaggcgga aatagaaaaa ggggatgagt ttttggagtg
     2701 ggtctggctt tacggcaagc cgattcgagt tgaagagctg gagcaacaat ccaaatccct
     2761 caattcgtct tttttaaaag gcggttatct aaatagcata aacacaagag tgctcgttcc
     2821 tctcatcacc aaaagaaagt tgataggggt gttgctttta ggagagaaaa aagataaaaa
     2881 gccatatact aatgaggagt tggtcttgct tgaggcagtg gctcatcaat tagccatagc
     2941 tatcctgaat gcgaagatga gtgaggaatt attgctctcc aaggaaatgg agtcgtttca
     3001 taaattatcc tcttttgtag tgcatgattt gaaaaacgcg atctctatgc tttcgatgct
     3061 attgcagaac gccaaggata atatgcacga ccctaatttt caaaaaggcg ccctattaac
     3121 tgtctccgat gcagtggaga ggatgcgggg actggtaacc aaaatatcct ctccttccaa
     3181 ggagatgcaa ttggatatat ccgagtgtaa tcttagtgag ttgattaaaa aggtggtaac
     3241 aggtatagac cctctgaatt tccccaggat aaagttcaac ttggatctga acgatacagc
     3301 tatggcccgc ttagatgagt cgcagatttc aaaggtttta cagaatctca taatcaatgc
     3361 tgtcgagtct atgccaccac aggggggagt tatagatatc agcgtctatg tcaaggatta
     3421 cttctggtgt gttgcggttt cagacagcgg agttggaatg tccagagaat tcatgaaaga
     3481 caagctcttc aagccgtttc agagcaccaa aaaaaagggg ttgggaattg ggctttatca
     3541 gagcaaggaa atagttgaag cacacggtgg taaaattgaa gtggagagcg ctgaaggaaa
     3601 aggggcaact tttgagatta ggttgccgat agataaatag atagcatagc gtttggttaa
     3661 caaaatatta cttgcatttc tgtcattctg accccgcctt tggcggggga agaatctcgc
     3721 ttttagacca gaagcgagat tcttcacgga gcctgtcctg agtgaaacga atgggttcag
     3781 ggtgacattt ttcagtagat attttcatag tgttatttgg ttgacatcgc agtatagaag
     3841 aaagaaatac aaaatgaagg ataagatact tattgtagat gatgacgaag gaatacgcac
     3901 ccaactaaat tgggcattgt ccggtgagta tgacgtcttc ttagctgaaa atgccaacca
     3961 ggccctggag attgctaaaa aagagaagcc cgacttggtg gctttggaca taaccctttc
     4021 tccgctggag gaagaaaaga gtggactgga cataatagag tccttcttgg aaatcgaccc
     4081 gctggtcaaa atcataatga taaccggtca cgatgaac
//
LOCUS       DASWLN010000102        24476 bp    DNA     linear   ENV 20-FEB-2024
DEFINITION  MAG TPA_asm: Candidatus Zixiibacteriota bacterium isolate
            SMAG_U2949 k141_982644, whole genome shotgun sequence.
ACCESSION   DASWLN010000102 DASWLN010000000
VERSION     DASWLN010000102.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37960594
            Sequence Read Archive: SRR10376091
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Candidatus Zixiibacteriota bacterium (soil metagenome)
  ORGANISM  Candidatus Zixiibacteriota bacterium
            Bacteria.
REFERENCE   1  (bases 1 to 24476)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 24476)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (26-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/12/2024 08:28:26
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 1,440
            CDSs (total)                      :: 1,416
            Genes (coding)                    :: 1,412
            CDSs (with protein)               :: 1,412
            Genes (RNA)                       :: 24
            rRNAs                             :: 1 (16S)
            partial rRNAs                     :: 1 (16S)
            tRNAs                             :: 21
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 4
            CDSs (without protein)            :: 4
            Pseudo Genes (ambiguous residues) :: 0 of 4
            Pseudo Genes (frameshifted)       :: 0 of 4
            Pseudo Genes (incomplete)         :: 3 of 4
            Pseudo Genes (internal stop)      :: 1 of 4
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..24476
                     /organism="Candidatus Zixiibacteriota bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_982644"
                     /isolate="SMAG_U2949"
                     /isolation_source="soil"
                     /db_xref="taxon:2053527"
                     /environmental_sample
                     /country="USA: Oregon"
                     /lat_lon="42.99 N 123.63 W"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            <1..382
                     /locus_tag="VGB16_03400"
     CDS             <1..382
                     /locus_tag="VGB16_03400"
                     /inference="COORDINATES: protein motif:HMM:NF020252.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=2
                     /transl_table=11
                     /product="HDOD domain-containing protein"
                     /protein_id="HEX9916760.1"
                     /translation="NKLFIQKVEEKVVKEDLSYREAETEIFSFDHAQVGALLVKKWNF
                     SQELEKVIEFHHEPYKAKGIVELAAITNFSDKLCNKLRIGFKKDASIVLEELESAVIL
                     NLQLPRVTELENELMQALAEEEHF"
     gene            501..1862
                     /locus_tag="VGB16_03405"
     CDS             501..1862
                     /locus_tag="VGB16_03405"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_013823608.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_process: GO:0006355 - regulation of DNA-templated
                     transcription [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="sigma-54 dependent transcriptional regulator"
                     /protein_id="HEX9916761.1"
                     /translation="MPSILVVDDKDTIRQMLSKTLQSEGYEVDVAEDGSIALEKAKDK
                     QYDLVLTDLKLPDKDGLEVLAALKDLDPEVAVVVMTAYGTIESAVQAMKMGAFDFLTK
                     PFDTDHLNMLIKRALETRRILAENILLREELAQSLGFTEIVGKCEKMKEVSRLISKVA
                     PSDTTVLVLGESGTGKELFARAIHRLSPRTEAPYVAINCAAIPRELLENEFFGSEKGA
                     YTGAYARKMGKFEIAEGGTIFLDEVGDLDVALQAKLLRVLQEKCFERLGGTKTISVNI
                     RLIAATNSDLKKGIEKKTFREDLYYRLSVFPITIPPLRERNEDIIELANYFIRKYCKE
                     MKKPMKGLSREGVSLMDKYHWPGNVRELENTIERAIILCEGKKILPEHLAIRVSTPAE
                     IRLREGAGLKEVGQYAQMEAEKSFILRVLNQSRGNKRKTAEILKIDYTTLFEKIKKYE
                     IEA"
     gene            2202..2684
                     /locus_tag="VGB16_03410"
     CDS             2202..2684
                     /locus_tag="VGB16_03410"
                     /inference="COORDINATES: protein motif:HMM:NF023444.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0015627 - type II protein secretion
                     system complex [Evidence IEA];
                     GO_process: GO:0015628 - protein secretion by the type II
                     secretion system [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="GspH/FimT family pseudopilin"
                     /protein_id="HEX9916762.1"
                     /translation="MKEKKLGKRGITLIELLTVIVLIGIIAAMAVPNFTNAVIKIRHK
                     SAARDLLGKIRMARSYAISRGVRYGVYMNTVEKYYITFRDVNSDGNYVAGTDSVKDST
                     SLDANLSFGACTFANNAVVFKNDGSTSESDSVEIINDKNSQKIKVHVTGAVGRAWLSQ
                     "
     gene            2967..4016
                     /gene="pilM"
                     /locus_tag="VGB16_03415"
     CDS             2967..4016
                     /gene="pilM"
                     /locus_tag="VGB16_03415"
                     /inference="COORDINATES: protein motif:HMM:TIGR01175.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_process: GO:0009297 - pilus assembly [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="type IV pilus assembly protein PilM"
                     /protein_id="HEX9916763.1"
                     /translation="MLPGKKGRTVTALDIGGNSIKLVKLEDKAGSYVLTALGIRELAA
                     EVIVDEEIKDRDALIFNIQSLIDQSGARTKDVAISISGHGVITDKIVMDRKTGAEAEQ
                     AILFEAEQRSPFDVEDVTMDYHIIKEDPELNKMEIVLVAARNEFLRSYLDLIMDAGLR
                     PAIVDIDAFAIFNAYEINYEVDPKKVTALIDIGFDTTTVTFIKDGMYHSTRDVASGGR
                     FIFNTMMREFRLNQELAFKAMKGEMGSSIDQDMFKATVITASEELISGLEVALSYFKS
                     SAKIPEIDWIVLSGGGGLIPFLPEFIQAKLNIPIEIANPLRNIEFDAEIFRAVQTEKI
                     APLLAVAVGLAARRI"
     gene            4020..4655
                     /locus_tag="VGB16_03420"
     CDS             4020..4655
                     /locus_tag="VGB16_03420"
                     /inference="COORDINATES: protein motif:HMM:NF016992.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="PilN domain-containing protein"
                     /protein_id="HEX9916764.1"
                     /translation="MIEINLLPKELRRGRRPFLVVDKTLSYMLIGTAVIIAFMVLIAA
                     WQNITKKNLGRAMVRSQVEIARLQEEIKEVDRLNALIERINQRRSAIEDLDRNRSAWV
                     DIMQDLSSRIPEYLWLSSFREEVAASSDSSAGSGIFTRSKVVVDGYAYSINSLASFII
                     QLGKSDYFKNIELKFVKQSILEQRKAFSFQLSGEILYSSEVAKRETENIEN"
     gene            4684..5256
                     /gene="pilO"
                     /locus_tag="VGB16_03425"
     CDS             4684..5256
                     /gene="pilO"
                     /locus_tag="VGB16_03425"
                     /inference="COORDINATES: protein motif:HMM:NF016253.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_process: GO:0043107 - type IV pilus-dependent motility
                     [Evidence IEA];
                     GO_process: GO:0043683 - type IV pilus assembly [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="type 4a pilus biogenesis protein PilO"
                     /protein_id="HEX9916765.1"
                     /translation="MDIRDPRNQVRFIFILVLCFVIFLWGKNIFFRNIQEIRNKNNQQ
                     ENLNKQLVAFQAKKQTIEGLREESEAREREYKSLELLLPEERQIPLFLTQMHGAAQTT
                     QTEIIQITPVGTATVSFYNVDNFNVEVVSNYHGFGRFLSNIANFPFIANISNISMTGL
                     STAEQENENKSIRVSFKLTTYYIKEGEKLK"
     gene            5269..6489
                     /locus_tag="VGB16_03430"
     CDS             5269..6489
                     /locus_tag="VGB16_03430"
                     /inference="COORDINATES: protein motif:HMM:NF023169.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="AMIN domain-containing protein"
                     /protein_id="HEX9916766.1"
                     /translation="MKKIFYIALIYLLLCCVWSEVWAECKVNNLSLERDSTFTKVVIY
                     ADIPFEYEHLTVEAKDGKPYRIVVDIADAIHALSQNNFLYIPSQTITAIRTSQFTTEP
                     NRKVRIVIDVRSPIVYKIEKEKENQLSLIISTPQDSNFTLWAADMSSLKGKETKLAQA
                     PQIEEKKQKDVPKLAEKEVKSTEAKQVIQAQAKEKEEKPKATVVEKKEAKPAEKIIAP
                     KTEEKKQVKTASKSNIVPAEKPPQAQKAKPTEEIKEEAKVEAESTTTETSQVAQLKVK
                     AEKTAEAIKSQVEKIKPRGEVIEAVYKRKVITYGSEDQRDPFTPIGEEIKVEFGKAPQ
                     PAYESLKLVGILKDVSGNMALLEDGEGYGYIMRQGDKVRNGEVIYVGDDRVLFQIVEF
                     GWSKTVAIELSKEK"
     gene            6506..7789
                     /locus_tag="VGB16_03435"
     CDS             6506..7789
                     /locus_tag="VGB16_03435"
                     /inference="COORDINATES: protein motif:HMM:NF012485.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="secretin N-terminal domain-containing protein"
                     /protein_id="HEX9916767.1"
                     /translation="MRKRMKSKIGKLIGTILLVGVLCSLVYSQSSSPRLIKSLILQNA
                     DIHSVLDFLAEYGGVNIVVAPNVAARVSLSLKNVTWREALDVVLKIHGLTAVEEPNYL
                     RVLLTGDYMKEQSELKVHELSQEALAPLVTEVIKIRNTAANDISNSLKGVLSGRGTVI
                     VDSRTNSVVIRDIYEGVEKAKNLITVLDKETDQIKISAQLLDVQSGAIKELGVNWTLI
                     PKLEGASNMTISQQANKVSDPIGNFTYKTFQTDFDLDGAISAMVSANKARVVAHPEIT
                     TVDNTEAFVQMGQKIPIKMFDQAGNIAIQLYEVGVILRVTPHITAENRILMKLEPERS
                     SYQFDPNGIIINTSNAKTTVVVDNGQTTVIGGLTSQEEKEVRTGLPILKDIPLLGKLF
                     SYTRKEVTNRDLVIFVTPTIVTKELKTPTVGEGEF"
     gene            8008..8091
                     /locus_tag="VGB16_03440"
     tRNA            8008..8091
                     /locus_tag="VGB16_03440"
                     /product="tRNA-Leu"
                     /inference="COORDINATES: profile:tRNAscan-SE:2.0.12"
                     /note="Derived by automated computational analysis using
                     gene prediction method: tRNAscan-SE."
                     /anticodon=(pos:8042..8044,aa:Leu,seq:caa)
     gene            8219..9454
                     /locus_tag="VGB16_03445"
     CDS             8219..9454
                     /locus_tag="VGB16_03445"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_018205747.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="saccharopine dehydrogenase C-terminal
                     domain-containing protein"
                     /protein_id="HEX9916768.1"
                     /translation="MKALVFGGTGKVGSAVAWDLSKDGEIKKVGIVGRHKDTLEKTKK
                     WIGSEKVVAHVLDITNKNETHKLMKQYDVGLIALPDRKTSYKVIHSAVEAGLNIVDML
                     EEYHRRPDPYETEGLEIPKGMTLDEYGDWLHNRAIESDVTFVDGMGFAPGLSNVTVGE
                     GMRKMDATEKAVARVGGIPSKEAAQRHPLRYMITWAFEHVLREYMIKLKVIKNGKVVE
                     VDAGTDRESFRFNQFGKDEELECAITPGMPSFLYTRPELKEFTEKTVRWPGHWQGVQT
                     LKECGLLDLAPVEFKGTKIMPREFLLSMITPRLRALEGDTDVCVMYNTLIGTKDGKKT
                     RTEYFMWDEADKKNGISSMARVTGFPVAITGKLIMAGKIKEKGIVPPEDCIKGKLYDE
                     FMQELKKRNINILEVTKPI"
     gene            9789..10175
                     /gene="acpS"
                     /locus_tag="VGB16_03450"
     CDS             9789..10175
                     /gene="acpS"
                     /locus_tag="VGB16_03450"
                     /EC_number="2.7.8.7"
                     /inference="COORDINATES: protein motif:HMM:TIGR00556.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008897 - holo-[acyl-carrier-protein]
                     synthase activity [Evidence IEA];
                     GO_process: GO:0008610 - lipid biosynthetic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="holo-ACP synthase"
                     /protein_id="HEX9916769.1"
                     /translation="MTFSKKFSHNTSIGVDITDISRFAKLNPKKDRLFLGRIYTPAEI
                     KYSFSKAKPAQHLAARFAGKEAVAKALKGLETVAYYKQIEITNDESGIPKAKLKNKRL
                     LNKFDVLLSLSHCEDKAIAFAIACKK"
     gene            complement(10178..10810)
                     /locus_tag="VGB16_03455"
     CDS             complement(10178..10810)
                     /locus_tag="VGB16_03455"
                     /EC_number="2.1.1.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_013328392.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008168 - methyltransferase activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="class I SAM-dependent methyltransferase"
                     /protein_id="HEX9916770.1"
                     /translation="MIKILLLLLGLFLLWQILIRIARKLFHFPAPAFIGHLLDSNYRR
                     ALQSPEKLIQRSGIKEGMQVLDLGCGSGAFTTFVARSVDPKGQVYALDIQPKMLEQLR
                     QKLSKPENQDIKNVELIESSAYDLPFENNSLDVVYMVTVLQEIPDKNKALGEIRRVLK
                     SGGILAVTELLPDPDYVFKSATAKLCQKAGFVLDEVSGNFWNYTARFKKP"
     gene            complement(11025..12275)
                     /gene="kbl"
                     /locus_tag="VGB16_03460"
     CDS             complement(11025..12275)
                     /gene="kbl"
                     /locus_tag="VGB16_03460"
                     /EC_number="2.3.1.29"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_010528869.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008890 - glycine C-acetyltransferase
                     activity [Evidence IEA];
                     GO_process: GO:0006567 - threonine catabolic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="glycine C-acetyltransferase"
                     /protein_id="HEX9916771.1"
                     /translation="MMAYSGKTKEFYQSELKGIKEAGLNKEERYIRSPQAADIKVEFP
                     EGAPEKEVVNFCANNYLGLSSHPEVVKAAHQGLDARGYGMSSVRFICGTQDIHRELEK
                     KLTDFLGTEATILFPSCMDANAAVFEVLLTPQDAMIADKLVHASIVDGMKLCKAQTYV
                     YKHCNMEHLEEKLKEAQENRFRMIITDGVFSMDGDIAPLDKICDLAEKYDAMVLVDDS
                     HSTGFVGKTGRGTHEHCNVMGRVDLITTTLGKALGGASGGCVSGRKELVDLLRQRARP
                     YLFSNTVPPVIVSAASKVLDLISKTTERRDKLEKNTLYFRKKMKEAGFDFREGTHPIV
                     PIMLYNAKLASDFAKDLYQEGIYVIGFFFPVVAKGQARIRVQISANHETKHLDKAIAA
                     FTKIGKKYDILGKTKDQIIQKYGM"
     gene            complement(12343..12600)
                     /locus_tag="VGB16_03465"
     CDS             complement(12343..12600)
                     /locus_tag="VGB16_03465"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+.
                     GO_function: GO:0000036 - acyl carrier activity [Evidence
                     IEA];
                     GO_process: GO:0006633 - fatty acid biosynthetic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="acyl carrier protein"
                     /protein_id="HEX9916772.1"
                     /translation="MQPDEAKQMIIDYVKKEFLEDDSDMKITETTPLISSGIVDSFSM
                     VSLKTFLEKKYEIQIPDAEATPEAFDTVNNIMNLLKKFSKI"
     gene            complement(12921..13292)
                     /locus_tag="VGB16_03470"
     CDS             complement(12921..13292)
                     /locus_tag="VGB16_03470"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_015836982.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF559 domain-containing protein"
                     /protein_id="HEX9916773.1"
                     /translation="MKSNLTTIAKNLRKRPTNAETLLWKHLRSKQLEGLKFRRQQAIG
                     NYVADFVCFEKRILIELDGGQHKKDNDNRRDKWFEKQGFKVLRFWNNDVLMNTRGALE
                     TIREECLYHPPLTPPIKGGEI"
     gene            complement(13377..15077)
                     /gene="acsA"
                     /locus_tag="VGB16_03475"
     CDS             complement(13377..15077)
                     /gene="acsA"
                     /locus_tag="VGB16_03475"
                     /EC_number="6.2.1.1"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_012960098.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="acetate--CoA ligase"
                     /protein_id="HEX9916774.1"
                     /translation="MSNIGSYEERYKDFSWKISEEELGFGKESSSNIGYFCSDRICEK
                     GLGKKLALIWEGYTGEVKKYTYDDVRIYSNSFAKFLKEIGIAPGDRVCIFMDRIPELY
                     ISFLGILKMGGIAQPLFSAFGEEALFTRLDDAKTKAILTTKKHVRKVRRIKETLPELT
                     KIIVVDADDFKLEKNEIPFSMEKYPKTEKFDIVKVEAETPSVLHYTSGTTGKPKGALH
                     VHSSIIAQYITTKWVLDLMSDDIYWCTADPGWVTGTSYGIIGPWANGVTQVVLDSGFS
                     AENWYAFIQRRKITVWYSAPTAIRLLMREGTEVVKKHDLSSLRYLASVGEPLNAEAVI
                     WSEKAFGKPFYDTYWQTETGSIVISNFPDMEVRPGSMGKAFPGIVATVVDPKTFEPIK
                     RPGSVGLIALKPGWPSMFRNYWNNKATYDSKFKNGWYICGDRSSIDKDGYFWFVGRDD
                     DVINTAGHLVGPFEIESALLEHPAVAESAAVGKPDPINMEVVKAFVALKPGFEKSDDL
                     ELDIMNYIRKKLSPLAMPQEIEFVRSLPKTRSGKIMRRLLRAQEWGETIGDISTLEND
                     "
     gene            complement(15424..16488)
                     /gene="tdh"
                     /locus_tag="VGB16_03480"
     CDS             complement(15424..16488)
                     /gene="tdh"
                     /locus_tag="VGB16_03480"
                     /EC_number="1.1.1.103"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_007505956.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008743 - L-threonine 3-dehydrogenase
                     activity [Evidence IEA];
                     GO_process: GO:0006567 - threonine catabolic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="L-threonine 3-dehydrogenase"
                     /protein_id="HEX9916775.1"
                     /translation="MEDSPVAQKMMAVIKKQKARGAELVEVDIPKLEPNEVLVKVKAT
                     SICGTDVHIYNWDRWAENRIKPPMIFGHECAGEVVETGKLVKGIKNGDYVSAETHIPC
                     GICFQCKTGNQHICQNLKILGVDINGCFAEYVKFPEICAWKNDKSLPFETATCMEPFG
                     NATYTVMESQVSAKIIAIFGDGPIGAFAVGIAKAVGAGKVFAVGEFPFRVNILKKMGA
                     DVALSIKEHNALDEIMKKTNGEGVDVVLEMSGAQKAIDDGLKILKKGGTFTAFGLPSG
                     RVDIDLANGIIFKGAKMIGINGRIMFTTWYQASNLLSAGLVDIRPVITHKFALKDFEK
                     GFSAVNSPQKEVGKVVFLTE"
     gene            16772..18040
                     /locus_tag="VGB16_03485"
     CDS             16772..18040
                     /locus_tag="VGB16_03485"
                     /EC_number="1.4.1.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_020708978.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0000166 - nucleotide binding [Evidence
                     IEA];
                     GO_function: GO:0016491 - oxidoreductase activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="Glu/Leu/Phe/Val dehydrogenase"
                     /protein_id="HEX9916776.1"
                     /translation="MKRLKSDDIQISSFENAMTQFDQAAKRMKLDPGLLEIMKQPRRS
                     VIVKLPVHMDDGSFRLFTGYRVQHSNVRGPAKGGIRYHQDVTLDEVSALAAWMTWKCA
                     VVGIPFGGGKGGIVCDPTKMSKSELERLTRRYTSELIDVLGPESDVPAPDVNTNEQTM
                     AWIMDTYSMHARHTVTSVVTGKPLDLGGSLGRVQATGRGVLFTIREAVKHLGMKMENA
                     KVVVQGFGNVGSVAANLLYDDGCKIIGVSDVKGGIHNPKGIDIKELLLHIKKTRSVVK
                     FPGTKPISNQELLELECDILVPAALENQITEENASRVKAKIIAEGANGPVIPEADKIL
                     DKRGVFVIPDILCNAGGVTVSYFEWAQDRMGLFWSEEEVNHRLEEIMKKSFGDVLEIS
                     CAKKVNMRTAAFMLAIKRVLDVFVKRGVYA"
     gene            18647..19996
                     /gene="gdhA"
                     /locus_tag="VGB16_03490"
     CDS             18647..19996
                     /gene="gdhA"
                     /locus_tag="VGB16_03490"
                     /EC_number="1.4.1.4"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_017497673.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016639 - oxidoreductase activity, acting
                     on the CH-NH2 group of donors, NAD or NADP as acceptor
                     [Evidence IEA];
                     GO_process: GO:0006520 - amino acid metabolic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="NADP-specific glutamate dehydrogenase"
                     /protein_id="HEX9916777.1"
                     /translation="MARDVSARVEAFMDKIIKKNPGEVEFHQAVREVAESLMFFLEEN
                     PIYQEAKILERIAEPERVILFRVPWMDDKGEIQINRGYRIEMNSAIGPYKGGLRFHPT
                     VNLGILKFLAFEQVFKNALTTLPMGGGKGGSDFDPKGKSDNEVMKFCQSFMLELQRHI
                     GPDTDVPAGDIGVGGREIGFMFGQYRRIRNEFTGVLTGKGLNWGGSLIRPEATGYGCV
                     YFVEEMLKTKGDSIKGKICTVSGSGNVAQYTVEKVNQLGGKCVTLSDSNGYVYDPAGV
                     DAKKLEFVIELKNVRRGRIKEYADKFGCKYVDGKRPWEVKCDVAFPSATQNEINEEDA
                     RMLVKNGCKAVGEGANMPTTFEGTQVFLNAKILFGPGKAANAGGVATSGLEMSQNSQR
                     LSWTREEVDAKLHGIMKAIHQQCVKYGKEKDWVNYVKGANIAGFMKVANAMLDQGLV"
     gene            20489..22216
                     /locus_tag="VGB16_03495"
     CDS             20489..22216
                     /locus_tag="VGB16_03495"
                     /inference="COORDINATES: protein motif:HMM:NF014567.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ATP-binding protein"
                     /protein_id="HEX9916778.1"
                     /translation="MKKRLSNKINTDISNHKLTENLRALSHQVLYYANLGIPRIDFMR
                     EVSRMLIDFSRCDVVEMRIKRSDKYFLCEAVRGNKVSFRIDHLSCAQVEKDKMIPCSQ
                     SDSGIETLCHNIMLKEFDSSSPFFTKNGSFWTSNVDDLLTTQSNNLRLDGEYKSIALF
                     PLAVNEENIGLLQLKSKQRDYFTEKEIELYEGFAQTLGVALAHRHAQVALRERIKELT
                     CLYGIARLAARTDLSLDEILQKITELLPPAWLYPEIASGKISLNGYSYATPDFREGLH
                     IQKSDIVVKGGKRGLVEVTYAEEKPELDEGPFLKEERSLIDTIAKDVALIIERRQAEE
                     EKVNLQNQLMHADRLATIGQLAAGVAHELNEPLSNILGFAQLAKKIPKLSKQTIGDLE
                     KIESASLHAREIVKKLMIFARQMPPKKTRVNLSQLVEEELYFFESRCAKEGIELIRLL
                     APKLPKIIADAGQLHQVLVNLVVNSIQAMPRGGKLTIQTSAGDNYISLIVKDTGIGMN
                     EEVLKHLFVPFFTTKEVGEGTGLGLSVVHGIISSHQGSIEVKSEVGNGTSFEIHLPIK
                     TQPHPKESI"
     gene            22218..23573
                     /locus_tag="VGB16_03500"
     CDS             22218..23573
                     /locus_tag="VGB16_03500"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_015336343.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_process: GO:0006355 - regulation of DNA-templated
                     transcription [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="sigma-54 dependent transcriptional regulator"
                     /protein_id="HEX9916779.1"
                     /translation="MASSPKKERILVVDDNPNTLEVLNRNLTSQGYQVLIAPGVAEAI
                     KILDGTLVDLVITDLKMPKVSGLDLIRHIRENFKETEVMMITGYASIQGAVKAVKTGA
                     EDYLAKPFTDEELFSAVRRSLDKLHSRRTRQDQTHHISTTSYGLIGESEAMHRVLGAI
                     TKAASTSATVLITGESGTGKELVARAIHYNSSRVSSSFVPVNCGGIPETLLESELFGH
                     VKGSFTGATESRAGFFQTADGGTIFLDEISETSLSMQVKFLRVLQDKEVYMVGATRPR
                     KVNVRIIAATNKDLRNLVKKEVFREDLFFRLNVITIAVPSLRDREDDILLLIDHFASK
                     FGKEFDKPVLRFSDEALQVLKNYDWPGNVRELENMVQRLVVMTDSYMIDVPDLPSPMR
                     FSALRDVGLNRTLAEVEAEYIRNVLATVDGNKTKASHILDIDRKTLREKLQKTKLSNK
                     P"
     gene            23794..24159
                     /locus_tag="VGB16_03505"
     CDS             23794..24159
                     /locus_tag="VGB16_03505"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEX9916780.1"
                     /translation="MTVYQAIKKVQKKEALSKREQKKETLSKKGEYAGLCLTCVNASG
                     CIYIKNQKSVVLECEDFEGYTNSPMRATINDVLSKVSSDASKADFGKYEGLCVNCEIR
                     ETCEFPKSEGGVWHCEEYQ"
     gene            24217..>24476
                     /locus_tag="VGB16_03510"
     CDS             24217..>24476
                     /locus_tag="VGB16_03510"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_014791856.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016491 - oxidoreductase activity
                     [Evidence IEA];
                     GO_process: GO:0006520 - amino acid metabolic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="Glu/Leu/Phe/Val dehydrogenase dimerization
                     domain-containing protein"
                     /protein_id="HEX9916781.1"
                     /translation="MAKASVDSASSGKKGSVYDNVSWQFNKAADLMKLDQNIRHILAK
                     TTNEIVVNFPVRMDDERIEMFTGYRVQHNNALGPFKGGLRYHP"
BASE COUNT         7380 a         4888 c         5627 g         6581 t
ORIGIN      
        1 taataagctc ttcatccaga aagtggagga gaaagtggta aaagaggacc tttcctatcg
       61 tgaggcggaa actgagatct ttagctttga ccacgcgcag gtaggtgccc ttttggtaaa
      121 aaagtggaac ttctctcaag agttagagaa ggttatcgag tttcatcatg agccatacaa
      181 agccaaagga atcgtcgaat tagctgctat aaccaatttc agtgacaagc tttgtaataa
      241 gctgaggata ggttttaaga aagatgcctc catagtattg gaggagttgg aatcggctgt
      301 gattttgaat ttacagttgc ccagagtcac tgaactggag aatgaactaa tgcaggcttt
      361 ggctgaggag gaacattttt gataggccta tagagaacgg attggctcaa aaaagttttg
      421 gataaaactt caactcctta ggggaatgtt ccgataaagt gcatagatga taagtatgga
      481 aatataagaa aggggttgtc ttgccttcca ttttagtagt agatgacaaa gatacaatca
      541 gacagatgct ttccaagacc ctgcaatctg aaggatatga agtggacgtg gcagaggatg
      601 gctcaattgc tctggaaaaa gcaaaagata agcagtatga tttggtcttg actgatttga
      661 agttacctga caaggatgga ctggaagttc tcgccgcctt gaaagatctg gatccggaag
      721 tggcggtagt cgtcatgacc gcatacggaa ctattgaatc agcagttcaa gctatgaaga
      781 tgggagcctt tgattttcta accaagcctt ttgataccga ccatctgaat atgctaatca
      841 aacgtgccct ggagacgcgt cggatattag cggaaaatat ccttttgaga gaggagttag
      901 ctcaaagctt aggctttacc gagatagtgg gaaaatgcga aaagatgaaa gaggtttcca
      961 ggttaatcag caaggtagca cccagcgata ctacagttct cgttctgggg gagagcggta
     1021 ccgggaagga gcttttcgct cgagctatac accgtttaag tcccagaacg gaagccccct
     1081 acgtagccat taactgtgca gccatacctc gggagctttt ggaaaatgag tttttcggct
     1141 cggagaaagg cgcttatacc ggggcttatg ccaggaaaat gggaaaattc gaaatagctg
     1201 aaggggggac aatctttttg gatgaagtgg gagacttgga tgtagccctg caagccaagt
     1261 tgcttagagt tctgcaggaa aagtgctttg agcgtctggg cggaacaaaa accatctccg
     1321 tgaacataag attaatcgcc gccaccaata gtgatttaaa gaagggtatt gagaagaaga
     1381 ctttcaggga ggacctgtat tatcgactct cagtctttcc aataaccata ccgcctttaa
     1441 gagaaagaaa tgaggatata atcgagttag ccaattattt catcaggaaa tactgcaaag
     1501 agatgaaaaa gccgatgaag ggtctctcgc gtgaaggagt ctctttaatg gacaagtatc
     1561 attggcctgg aaatgtacgc gaattggaaa acacgattga aagggcaatc atactatgtg
     1621 aaggcaagaa aatccttcca gagcacctgg ctataagagt gtcaactccg gctgagataa
     1681 gattgaggga gggggctggc ttaaaagagg tgggacagta tgcccagatg gaggcagaga
     1741 agagtttcat cttaagggtt ttgaaccaga gccgtggcaa caaacgcaag acggccgaga
     1801 tacttaaaat agactacact actttgtttg aaaaaatcaa gaaatacgag atagaggctt
     1861 gagcttgagt atagggagaa tccaatacaa tatagaagat gccctaactt tagttccact
     1921 ttggtcggat tttgcaataa taaaacggtt ggcttgacaa tttgctcttt ctaatctaaa
     1981 gtaaaacaag gagttaccat agatggcgga ttgatatgct cctcttcaga tacttggcac
     2041 aactattgct gattcttcaa tgcgggttaa actttaggga aaagaagaat ggagcataga
     2101 agcgttaatg atggtccgat tttggcaata gccttactgg taataatctt tgtggctttt
     2161 ttctttatca ctcggctagg ataaaaactt agataaaaat tatgaaggaa aaaaaattag
     2221 gcaagagagg aattactctc atagagcttt tgaccgtaat tgtgcttatc ggaatcatag
     2281 ctgctatggc tgtgccgaac ttcaccaatg ccgtaataaa gataaggcac aaatccgcag
     2341 ccagggacct gttaggcaaa ataaggatgg ctcgctccta tgccatctcc agaggggtaa
     2401 gatatggagt ctatatgaat actgtggaga agtattatat cacatttagg gatgtaaaca
     2461 gtgatggtaa ttatgttgct ggcacagaca gtgtaaaaga ttctacttcc ttagatgcca
     2521 acctctcttt tggtgcttgc acgtttgcca ataatgctgt ggtttttaaa aatgatggct
     2581 ccacttctga aagcgactca gtggagataa taaacgataa aaactcccaa aaaatcaaag
     2641 tgcatgttac gggcgcggtg ggcagagctt ggttatctca atgaccaaat acaaatttct
     2701 cttcttttct cgtcaagtat caaaaatgaa ttcaagttaa tgctttcagc atttttgccg
     2761 ataaataata aagaaagcgg aggttagatt ttggtctgta aaattgtttt gggaacactt
     2821 aagtattttg gtcaaaaggt cgataaaaaa aatagtgatt ttgttatatg gggtaaatcc
     2881 cataatcaag atttcgcttg acaaagaaac tcgtaaaaaa tatagtttga gataaggttg
     2941 ttcaaaattc aaacaagaga ttgaatatgt tgccaggtaa gaaaggcaga accgtaaccg
     3001 cactggatat cggagggaat tccattaagt tagtgaagct tgaagacaag gcgggaagtt
     3061 atgtgttgac tgctttagga atcagagagt tggctgctga ggtgatagtg gatgaggaga
     3121 taaaagatag agatgccttg atttttaata tccagagctt aatcgaccag tccggagcca
     3181 gaacgaagga tgtggctatt tcaatttccg gtcacggagt tatcactgat aagatagtga
     3241 tggatagaaa aacgggagca gaggcagagc aagctatcct atttgaggcg gaacaacgca
     3301 gcccctttga tgttgaagat gtgactatgg actatcatat aataaaggaa gacccagagc
     3361 tcaataagat ggagatagtt ctggttgcgg cgcgcaatga gttcctgcgt tcctatctgg
     3421 acctaattat ggatgcaggc cttaggccag ccatagtgga cattgatgct tttgccatat
     3481 tcaatgccta tgagataaac tacgaagttg accccaagaa ggtgactgcc ctcatcgata
     3541 tcggttttga cactactacc gttaccttca taaaggatgg gatgtatcat tccacgcgtg
     3601 acgtggcgag cggtggaaga tttatcttta ataccatgat gagggaattt cgtctaaatc
     3661 aggaattggc tttcaaagcc atgaaaggtg aaatggggtc ctccatcgac caagatatgt
     3721 ttaaggctac ggtcatcaca gcttcagagg aattgatctc tggtctggaa gttgctttgt
     3781 cctattttaa gtcttcagcc aaaattccag aaatagattg gattgttcta tccggaggag
     3841 gaggattgat tccatttttg ccggaattca tacaagccaa gttgaatatt ccaattgaga
     3901 tagcaaatcc cttacgcaat atagagtttg atgcagagat atttagagcc gttcagacgg
     3961 agaagattgc gccgctgttg gctgtggcgg tggggttggc agccagaagg atataacata
     4021 tgatagagat aaacttactt cctaaggaac taagacgagg gagaagacct tttttagttg
     4081 ttgataaaac cttgtcttat atgttgattg gcacagcagt aataatcgct tttatggtgt
     4141 tgatagcggc ctggcagaat ataaccaaga agaacctggg cagagccatg gtccggagcc
     4201 aagttgagat agctagactt caggaggaga taaaagaggt agatagattg aatgcgctta
     4261 ttgagagaat aaaccaaaga aggtctgcca tagaggattt agatcgcaac cgttctgcct
     4321 gggtggatat aatgcaggat ttgagctcac gcattccgga gtatctgtgg ctttcttcct
     4381 ttcgagaaga agtcgctgct tcttcagata gttcagctgg cagtggcatt tttaccaggt
     4441 ccaaagtggt cgtggatggg tatgcttatt ccataaacag tttggcgagt ttcataattc
     4501 agttgggaaa atccgactac tttaagaaca tagagctgaa attcgtaaag cagtctatac
     4561 tggagcaacg taaagctttc tcttttcagc tgtctggcga gatactttat tcttccgagg
     4621 tagccaaaag agagactgag aacatcgaga attaaaaagc aagtccgttt acggagggga
     4681 taaatggata tcagagaccc caggaatcaa gtaagattca ttttcatatt ggttttgtgt
     4741 tttgtcattt ttctttgggg gaagaacata ttctttagaa acatccagga gataagaaac
     4801 aaaaacaacc agcaggagaa tttgaataaa cagcttgtag ctttccaggc taagaagcaa
     4861 acgatcgaag gtttaagaga agaaagcgaa gccagggaaa gggagtataa gagtttagaa
     4921 ttgctccttc cagaggagag gcagatccct ctgtttctca cccagatgca tggtgcagct
     4981 cagacaactc aaaccgagat tattcagatc acaccagtgg gcacggctac agtcagtttc
     5041 tataacgtag ataacttcaa tgtagaggtg gttagcaact accatgggtt cgggcgattt
     5101 ctgagcaata tcgcaaattt cccttttatt gccaacatct caaacatatc tatgactggc
     5161 ttatctacag ctgaacaaga aaacgagaat aaaagcatac gggttagctt caagttaacc
     5221 acctactata taaaggaagg ggagaaactc aaataagtga gttcgaatat gaaaaagatt
     5281 ttttatatag cattaattta tcttctgtta tgctgtgttt ggtccgaggt ttgggcggag
     5341 tgcaaagtca acaacctaag cctcgagaga gatagcacct ttaccaaggt ggtcatctat
     5401 gcggacatac cgtttgagta tgagcatctt acggttgagg ctaaggacgg aaaaccctat
     5461 cgcatagtgg tagatattgc agatgccatt cacgccctgt cccaaaacaa ttttctctat
     5521 ataccgagtc agactattac tgccatccgc accagccagt tcacgactga gccaaacagg
     5581 aaagtcagaa tagtcatcga tgttagaagc ccgattgtat ataagataga gaaagaaaaa
     5641 gagaatcagt tgagtttaat tatctccact cctcaggata gcaacttcac gctttgggca
     5701 gcggatatgt ctagccttaa agggaaagag acaaaattag cacaagcgcc acagatagaa
     5761 gagaaaaagc aaaaggatgt gccgaagctg gcagagaaag aagtaaaatc gactgaagcc
     5821 aagcaggtga ttcaggctca agcgaaggag aaagaggaga aaccaaaagc gacagtggtt
     5881 gagaaaaaag aagcgaaacc tgctgaaaag ataatagctc cgaaaaccga agagaagaaa
     5941 caagttaaga cagcttcaaa gtcaaacatt gttccagcag agaaacctcc tcaagctcaa
     6001 aaggcaaagc caacagaaga gattaaggaa gaagccaagg ttgaagcaga atcaaccaca
     6061 accgaaacca gccaagtagc ccagttgaaa gttaaggcgg agaagacagc tgaggcgata
     6121 aagagtcagg tagaaaaaat caagccacga ggggaagtca tagaagcagt ttataagaga
     6181 aaagtcatca cctatggaag cgaggaccag agggatccat tcacgccaat aggtgaggag
     6241 ataaaagtag agttcggcaa agcccctcag ccggcttatg agagtttgaa gttggtgggg
     6301 atactaaaag atgtgtcagg aaatatggca cttttggagg atggtgaggg gtatggttac
     6361 atcatgcgcc agggagacaa ggtgagaaac ggagaggtga tctacgtggg ggatgataga
     6421 gtgctctttc agatcgttga attcggctgg tctaagaccg tagccataga actttccaaa
     6481 gaaaaataac aaggggagta atattatgag aaaaagaatg aaaagtaaaa ttggtaagtt
     6541 aatcggaacg attctgctgg tgggagtgtt gtgtagtctg gtctattcgc agagcagctc
     6601 ccccagactc atcaagtcgc tgatcttaca gaatgcggac atccattcag ttttggattt
     6661 tctggcagaa tacggtgggg tgaatatcgt agttgcccca aatgtcgctg cccgtgtaag
     6721 cttgagtctc aaaaacgtga cctggaggga agctctggat gttgttctca agatacatgg
     6781 cctgacagca gtggaggagc caaactattt aagagttctt cttactggtg actatatgaa
     6841 ggagcaatcg gaattaaaag tacacgaact tagccaggag gcattagctc ctctggtgac
     6901 ggaggtcatc aagattagaa acactgctgc caatgatatc tccaattcat tgaaaggtgt
     6961 gcttagcggt cggggcacag ttatagttga ttcgcgcacc aacagtgtgg taatacgcga
     7021 catctacgaa ggagtagaga aagccaaaaa cctgataaca gttctggata aggaaactga
     7081 ccaaattaag atatcagctc aactcttgga cgtgcaaagt ggagccataa aggagttggg
     7141 cgtgaactgg acacttattc cgaagcttga aggtgcttcg aacatgacca tcagccagca
     7201 agctaataag gtttcggatc caataggcaa ctttacctac aagacttttc agactgattt
     7261 tgacctggat ggcgctattt cggctatggt ttctgccaat aaggcgaggg tagtggccca
     7321 tccggagata accacggtgg acaacaccga agcttttgtg caaatgggac aaaagatacc
     7381 aataaagatg ttcgaccaag caggcaatat cgccattcag ctttacgagg taggtgttat
     7441 actgagggtt actccgcata taactgcgga aaacagaata ttgatgaagc tggagccaga
     7501 aagaagctct tatcaatttg atcctaacgg cataatcatc aacaccagca atgctaaaac
     7561 caccgtggtg gtagataatg gacaaaccac agtaatcggt ggtttgacct cgcaggagga
     7621 aaaagaagtc agaaccggtc ttcccatttt aaaagatata cctcttctag gcaaattatt
     7681 cagttataca agaaaagaag tcactaaccg tgatttggtg atttttgtta cccccacgat
     7741 tgtaacaaaa gagttgaaaa ctccaactgt gggggaagga gagttctaaa agagtcacaa
     7801 gagttgactc aaaagctttt ggatagactc caaaagcttt tttgtttgtg ccatttctat
     7861 tattagataa accctactac tcttggaata gttccctcga cactgatacg gagtctcctt
     7921 gagtcaaaag tccacttatt ccacaataga gataaagaac ttttgtcttg actttaaaaa
     7981 cagattgaat ttattttgtt tgaaattgcc ggagtgatgg aactggtaga cgtgccggac
     8041 tcaaaatccg gtgcccgcaa gggtgtgtcg gttcgattcc gacctccggc atgcaccgtt
     8101 ctggtgcata gctccggcat ataccagaac agtagacgtc tccggcatgt agcatatagt
     8161 acagattaac cacactgaaa ctaattatta aagaatcaat tacttcaagg aggaatctat
     8221 gaaagctttg gtgtttgggg ggacaggaaa ggtcggttct gcagttgcgt gggacttgtc
     8281 caaagacggc gagattaaga aggtgggcat cgtgggaagg cataaggata ctttagagaa
     8341 gacgaagaaa tggataggca gtgagaaggt ggtggcacat gtgctcgaca tcaccaataa
     8401 gaatgaaaca cacaagttga tgaagcaata tgatgttggt ttgattgcac tacctgatag
     8461 gaaaacaagc tataaggtca ttcattctgc agtggaagcc gggttgaaca tagttgatat
     8521 gcttgaagag tatcatcgca ggccggaccc atacgaaact gagggactgg aaattcccaa
     8581 gggaatgact ttggatgagt acggagactg gctgcataac agagccatag agagtgatgt
     8641 gacatttgtt gatggaatgg gttttgcacc tggcttgagc aatgtcacgg tgggtgaagg
     8701 catgaggaag atggatgcca cggagaaagc agtggctagg gttgggggta ttccttcaaa
     8761 ggaagctgca caaaggcacc ccttgagata tatgatcacc tgggcatttg agcacgtatt
     8821 aagagagtat atgattaagc tgaaggtgat aaagaacggt aaggtcgtcg aggtggatgc
     8881 tggcaccgat cgagaaagct tcaggtttaa ccaattcggc aaggatgagg agttggagtg
     8941 tgccatcaca ccaggaatgc ccagcttcct ttacaccaga ccagagctta aggagttcac
     9001 cgagaagacc gtacgttggc ccggacattg gcagggggta caaaccttaa aggaatgtgg
     9061 actgctcgac cttgcgccgg tagaatttaa gggaaccaag ataatgccgc gagagtttct
     9121 gttatccatg attacaccca gacttcgggc gctggaagga gataccgatg tttgcgttat
     9181 gtacaatacg ctaataggaa ccaaagatgg caagaagacc agaactgaat atttcatgtg
     9241 ggacgaggcg gacaaaaaga acggcatttc ctctatggca agggttaccg gattccctgt
     9301 agccataacc ggaaaactga ttatggcagg aaaaataaaa gagaagggca tagtgcctcc
     9361 ggaggattgc ataaaaggga agctttacga tgagttcatg caagagttga agaagagaaa
     9421 cataaatata cttgaggtca ccaagccaat atagcaggaa gatgtttcat tattagtagg
     9481 tcagaagctt tatgttgtgt caggtccttg aagcctgata cctggcagga gtagctcctg
     9541 acctatgatg tgttttggag tgcatcccgc catcggcggg atgcacgcat tgacacggtc
     9601 aatacactcc atattgcttt gtcaatgata tttggcttac gttcttgtca ttctgaggcg
     9661 tagccgaaga atctcgcttt tagacgagaa ccgagatcct tcacttcgtt caggatgaca
     9721 ttctaaatag atattatcat gcacgccatt cagttgacat ggcaacatat aaaaaaccat
     9781 aactgtttat gactttttcc aaaaaattct ctcataacac cagcatcggt gttgacatca
     9841 ccgacatatc cagattcgcc aagttaaacc caaagaaaga tagacttttc cttggcagga
     9901 tatatacgcc agctgaaatc aagtactctt tttccaaggc aaaaccagcc cagcatttgg
     9961 cagcacggtt tgccggaaaa gaggcggtgg caaaagccct taaaggattg gagactgttg
    10021 cttattataa gcagatagag ataaccaacg atgagtctgg aattccaaaa gcgaaactca
    10081 agaacaagag attattgaat aagtttgatg tactgcttag cttatcccac tgtgaagata
    10141 aagcgattgc ttttgcgatc gcgtgcaaaa agtgagttta cggtttcttg aatctggcag
    10201 tgtaattcca gaaatttcct gacacttcat ccaagacaaa tcctgctttt tggcataact
    10261 tggctgtagc tgacttaaag acataatcgg ggtctggcaa aagctccgtt accgcaagaa
    10321 ttccacccga ttttaatacc cttcttattt cccctaaagc tttattttta tcaggtatct
    10381 cttgaaggac tgtaaccata tagacgacgt caagagaatt gttctcaaag ggcaaatcgt
    10441 aggcactgct ttctattagc tcgacgtttt tgatgtcctg attttcaggt ttagataatt
    10501 tttgtcttag ctgctccagc atctttggct ggatgtccaa agcatagact tgaccctttg
    10561 ggtcaacact tctcgccaca aaagtcgtga aagcaccact tccacatccc aggtccaaaa
    10621 cttgcatccc ttcctttatc ccactgcgtt ggattagctt ctcaggggac tgtaaagccc
    10681 tcctgtaatt actatctaaa agatggccaa taaaagcagg ggctggaaaa tgaaaaagtt
    10741 tacgggctat cctaatcaga atttgccaca agagaaaaag ccctaaaagc agaagaagga
    10801 tttttatcaa tttcgagtca gtcgcaacct aatttttctg gaacgaaggt tactaataca
    10861 aacgcattaa tttacgttca ataaatctcc cctcgcccct cttttccaaa gaggggaaaa
    10921 aaagaggtct ccccctttga aaaaggggga ttaaagggga ttttaaaata gattgcatat
    10981 tgctctaata tgtaacaaca tttgatgcct ttgctatagt taccctacat tccatacttc
    11041 tgaattatct ggtctttggt tttgcccagg atgtcgtatt tttttccaat cttggtaaaa
    11101 gccgcaatgg ccttatccag atgtttggtc tcgtggttag ctgaaatttg cactcttatg
    11161 cgggcttgac ctttggctac gaccgggaag aaaaatccaa ttacatatat tccctcttgg
    11221 tacaaatcct tggcaaaatc ggaagccagc ttggcattgt aaagcattat aggaacaatg
    11281 ggatgagtac cctctctgaa gtcaaatcca gcctctttca ttttctttct gaaatacaaa
    11341 gtattcttct ccaatttgtc ccttctctcc gtggttttgg aaatcaaatc cagaactttg
    11401 ctggcagcgg agacgatcac cggagggaca gtgttggaga aaagatatgg tctagctctc
    11461 tggcgtaata ggtcaactag ttcctttcta ccactaacac agccgcctga agcccctcct
    11521 aaagccttac ctaaagtagt agtgataagg tccacccttc ccatcacatt gcaatgctcg
    11581 tgtgttcccc tgcctgtttt tcccacaaag ccagtagaat gcgaatcatc cactaaaacc
    11641 atagcatcat acttttcagc caggtcacaa atcttatcca gtggcgcaat atctccatcc
    11701 atagagaaaa caccatcggt gataatcatt ctaaaacgat tttcctgcgc ctcttttaat
    11761 ttttcctcca ggtgttccat attacagtgc ttgtaaacgt atgtttgagc tttacagagt
    11821 ttcatcccat ccacaattga ggcatgcacc agtttatctg caatcatagc atcctgcgga
    11881 gtcaaaagca cttcgaatac cgcagcattg gcatccatac aagaagggaa aagaatcgta
    11941 gcttccgttc ccaagaagtc ggtcagtttc ttttccaatt cgcgatgaat atcctgtgtg
    12001 ccgcagataa atctaactga agacataccg taacccctgg catccagtcc ctgatgagct
    12061 gccttaacta cttccggatg actggaaagt cccaagtaat tgttggcgca aaagtttacc
    12121 acctcttttt ccggcgcccc ttcggggaac tctactttaa tatctgccgc ctggggagag
    12181 cgaatatatc tctcctcttt atttagccct gcctctttta tgcccttcag ctcgctttga
    12241 taaaattctt tggttttgcc actgtaagcc atcataacct cctaaagtta cgagtatatc
    12301 ccaagttttc tgaagcggga gatgtttcga aactcccaaa gatcatattt tactgaattt
    12361 cttaagcaag ttcataatgt tgttcactgt atcgaaagct tccggagtcg cttccgcatc
    12421 cggaatttga atctcgtatt tcttctccag aaaagttttc aatgagacca tggaaaagga
    12481 atcaactatt ccggaggaga ttaagggggt agtttcagtt attttcatat cgctatcgtc
    12541 ctccaggaat tcctttttaa cataatcgat gatcatctgc ttcgcttcat caggttgcat
    12601 aaacttgctc ctttaaggtt tttgggttgt tttacaatct gcattattgt cgaaagagtg
    12661 atgctattta ttcaccctct tcttaatcct ctcccgtcga gggagaggat agaacccgtc
    12721 ttcttatgtt tgcccccagt cgttattgaa gattgcctta tatattttta acaacagcaa
    12781 agcccatcaa atccctttac tatctctcgg aaagagagga ttagatttat gttttatgtt
    12841 cacctcagat gtcatcaaaa atcgcttatc cattctcaac agtaagagcc atcaaatctc
    12901 tcctgcagca gcatgctctg tcaaatttcc cctcccttga tgggaggggt aaggggaggg
    12961 tgatataaac attcttctct tatagtttcc aatgctcctc ttgtgttcat caaaacgtcg
    13021 ttattccaga atctcaacac cttaaagcct tgtttttcaa accatttatc tcttctgtta
    13081 tcattatcct ttttgtgttg tccaccatct aattctatta gtatccgttt ttcaaaacaa
    13141 acaaaatcag ccacataatt tccgattgcc tgttgtcttc taaatttaag cccttctaat
    13201 tgttttgacc ttaaatgttt ccatagcaga gtctctgcat tagtaggtct ttttctgaga
    13261 ttcttggcta tggtggtaag attactcttc aacttcttta accaccctca ccctagccct
    13321 ctcccatcga gggagagggg atatagggga tcatctgttt attaacagta agtttgtcaa
    13381 tcattctcca gagtcgaaat atccccgatg gtttcgcccc attcctgtgc ccgtaagagt
    13441 cttcgcatta tctttccgct tctggtcttg ggtaatgacc ggacaaattc gatttcctgc
    13501 ggcatagcca aaggggataa tttcttccga atgtaattca ttatatccaa ttctaaatca
    13561 tcgctctttt caaaaccggg cttcagagct acaaaagctt tgaccacttc catattaata
    13621 gggtcaggtt tgcccacagc agcagattca gccacagctg gatgttccaa aagggcggat
    13681 tcaatctcaa acggtccaac caagtgtcct gcagtgttaa tcacatcatc atctcgtccc
    13741 acaaaccaga agtatccatc cttatctatg cttgagcggt ctccacagat ataccaaccg
    13801 tttttgaact tgctgtcata agttgcctta ttgttccagt aattcctgaa cattgagggc
    13861 cagccgggtt tcagggcaat aagcccaact gaaccgggtc tttttatggg ctcaaaggtt
    13921 ttagggtcaa ccactgtggc gacgattccg ggaaaagctt tacccattga gccgggcctg
    13981 acttccatat ccgggaagtt agaaataacg atgcttccgg tttcagtctg ccaataggtg
    14041 tcataaaatg gctttccaaa cgctttctcc gaccagatga ctgcctcagc attgagcggc
    14101 tcacccacgc tggctaaata tctcaaagag gaaaggtcat gttttttcac cacctctgtg
    14161 ccctctctca tcagcagacg aatggcagtt ggagcagaat accatacagt gatcttacgt
    14221 ctttgaataa aggcgtacca gttttcagcg ctgaaccctg aatccaaaac tacctgggta
    14281 actccgtttg cccagggacc aattatgcca tacgaagttc cagttaccca gccggggtca
    14341 gcagtgcacc agtagatatc atccgacatt aagtctaaaa cccatttggt ggtaatgtat
    14401 tgtgcgatga ttgaggaatg aacatgtaaa gcccctttgg gcttgccggt tgtgccggag
    14461 gtataatgca gaacagatgg agtttccgcc tcaactttaa ctatatcgaa tttttcagtc
    14521 ttaggatatt tttccataga gaagggaatt tcgttttttt cgagcttaaa atcatcggca
    14581 tccactacta ttatcttagt gagttcaggc agggtttcct taattcttcg aacctttcgg
    14641 acgtgctttt tggtggttag aatcgctttg gtcttagcat catctaatcg ggtgaagagt
    14701 gcctcttcgc caaaggctga aaatagtggt tgggcaattc cacccatttt tagaattccc
    14761 aaaaaggaga tgtaaagctc ggggatgcgg tccataaaga tacataccct atccccagga
    14821 gctattccaa tctcttttag aaacttggca aaagagttgc tataaattct tacgtcatcg
    14881 taagtgtatt tcttgacctc accggtatat ccttcccaga ttagggcgag ctttttgcct
    14941 aatccttttt cacagattcg gtcgctgcag aaataaccga tgttgctgga tgactccttg
    15001 ccgaacccga gctcttcttc tgaaatcttc cagctgaagt ccttgtatct ttcttcgtat
    15061 gaaccgatat tgctcataga aaatctccta taaagtcagt ctatagcaaa cgcattaaac
    15121 gttgttacat attagaggaa tatgcagtct attttgaaat ccccctcaaa tccccctttt
    15181 tcaaaggggg agacctcttt ttcccctctt tcgaaaagag gggcgagagg agatttattg
    15241 aacgtaaatt aatgcgtttg cattagaaag ttaagggttt gtcattttgt aggggctcga
    15301 tttatcgagc ccgttagttt cgggtcggat aaatccgacc cctacaaatt ttcttgatgc
    15361 aacccaagga aattcgtagg ggcgggctcc cacgcccgcc ctaatctttt aatgttctac
    15421 ctttcattcc gtcaaaaaca ccactttccc cacctctttc tgtggggaat taactgcact
    15481 aaatcctttt tcaaaatcct tcaaagcaaa cttatgagta atcaccgggc ggatgtcaac
    15541 caagccagca gagagtaaat tgcttgcctg ataccaggtg gtaaacatta ttctgccgtt
    15601 tattccaatc atcttcgcgc ctttgaatat aatcccattg gctaaatcta tatccaccct
    15661 tccggaaggc aatccaaagg cggtgaaagt cccacctttt ttcaatatct tcagtccatc
    15721 gtcaattgct ttttgagcac cgctcatctc cagaaccaca tccacacctt caccattggt
    15781 ctttttcata atctcatcca gagcattatg ctctttgatg ctcaaagcga cgtctgcgcc
    15841 catctttttt aagatattta ctctaaaagg aaattcaccc actgcaaaaa cctttcctgc
    15901 tccaactgcc ttggcaatac caactgcaaa agcaccaatc ggaccgtctc caaagatcgc
    15961 tattatttta gctgaaacct ggctttccat aacagtatag gtagcattcc caaagggctc
    16021 catacaagta gctgtctcaa aaggtaaaga tttatcattt ttccaggcac agatctccgg
    16081 aaatttaaca tattcagcaa aacatccatt tatatccact cccaatattt tcaaattctg
    16141 gcagatatgc tgatttccag ttttacattg aaagcagatt ccacaaggga tatgcgtttc
    16201 agctgaaaca taatctccat tcttaatccc cttaaccaac ttccccgtct ccaccacctc
    16261 tccggcacac tcgtgtccaa aaatcatagg tggcttgatt ctgttttcag cccacctgtc
    16321 ccaattgtat atatggacat cggtcccaca tatggaagtt gccttgacct tgaccaaaac
    16381 ctcattcggc tcaagtttgg gtatatcaac ttccaccagt tcagcccctc tggctttctg
    16441 ttttttaatc accgccatca ttttttgtgc cactggacta tcctccattt ttgaaacaca
    16501 aaaatataat agattagctt gggttgtcaa gcaaatacag attgtgaata ctgaagcatc
    16561 tgtagtttaa agacttaact tccgtcttca ataggctgaa aaattacaca aaatctgttg
    16621 acaaaaagtg ttagaccttt tatcttttga taaatttttc acaaggttaa acaagtattc
    16681 taatcaaatc cgttttggag atactaaaat cacacaaaat agcagtcgca caatttaggg
    16741 caactgcaaa aaaccaaatc aggagggaag gatgaagaga ttaaaaagcg atgatattca
    16801 aattagttca tttgaaaatg ctatgaccca gtttgaccag gcggcaaaac gcatgaagct
    16861 ggacccgggt ttgctggaaa taatgaaaca acctcgtagg tcggtaatcg taaagcttcc
    16921 cgtccacatg gatgatggga gcttcagact gttcaccggt tatcgggtgc aacattcaaa
    16981 tgtaagaggt ccggccaaag gaggtattcg gtatcaccag gacgtaactc tggatgaagt
    17041 ctcagccttg gctgcttgga tgacctggaa gtgcgctgtg gtggggatcc cgtttggtgg
    17101 cggcaaggga ggcatagttt gtgaccctac caaaatgtct aaaagcgagt tagagagact
    17161 aacccgacgc tatacttctg agttaattga tgttttgggt ccggaaagcg atgtgccggc
    17221 accggatgtt aacaccaatg agcagactat ggcttggatc atggatactt atagcatgca
    17281 tgctcgtcat acagttactt cagtagtaac cggaaagccc ttggatttag gagggtcgtt
    17341 aggtagagtg caagctactg gtagaggtgt tctttttacc attcgagaag cggtaaagca
    17401 tttaggtatg aaaatggaga atgcgaaagt agtggtgcaa gggttcggaa atgttggttc
    17461 cgtggcagcc aatctgctgt atgacgatgg atgtaagata attggagtaa gcgatgtcaa
    17521 aggtggtata cataacccca agggaataga catcaaagag ctcttgcttc atatcaagaa
    17581 aaccagaagc gtggtgaaat ttccaggcac caagccaatt tccaaccaag aacttctgga
    17641 attggaatgt gatattctgg ttcccgcggc tttggaaaac cagataaccg aggagaatgc
    17701 ctccagagtt aaggcaaaga ttatagcaga gggggcgaat ggaccggtta ttcccgaagc
    17761 tgataagata ctggacaaga gaggggtctt tgtgattcca gatatactgt gcaacgccgg
    17821 aggagtcaca gtctcctatt ttgagtgggc tcaggatcga atggggctgt tctggagcga
    17881 ggaggaagtt aatcatagat tagaagagat aatgaagaaa tccttcgggg atgtgctgga
    17941 gatctcttgc gccaagaaag tgaatatgcg aaccgccgct ttcatgctag ccatcaagag
    18001 agtgttagat gtttttgtga agagaggagt ctacgcttaa gtaagtctca gcttgaagct
    18061 ggaatcgatt tgttcgcttt tttagaagat ttttgtaata gtagggacag gtttcaaacc
    18121 tgtcccttta tattttcacg ataattaagg aaatatgatt attatggaaa gcccccgcat
    18181 tatgccaatg gggaatttct ccccaatttt cagatttttc aaagagaaac ggtagattat
    18241 tccccatttt tctcttgaca aaaggttaaa agcttatata ataaaaatat tttgggtatt
    18301 cgcttctttg aaaaactaag aaacttagct ggggcgagaa ataaaaagat aaaagcccag
    18361 tgcagacaaa agagatagaa agttctatat catgcttgag atggtttaag aaattgtttt
    18421 gcatcttgca ttaagattcg cacagatatt atggagttgg ctttttttta gattttgctt
    18481 gttacacatt tcacaaacaa gacccgaaag gaagatatta acaagggatg ataagcctta
    18541 aagaaataaa gaaaggagga acgcgtagat taaaaggtag aatcgtttta agggtcggga
    18601 tattaaatca cagacaacaa acccaaaaca ataaggagat tttgttatgg caagagatgt
    18661 ttctgcaaga gtagaagcgt tcatggataa aatcattaag aaaaatcctg gagaagtgga
    18721 gttccatcaa gcagtcagag aagtggctga atctttgatg tttttcctgg aggagaatcc
    18781 gatataccag gaagctaaaa ttctggagag gattgcagag cctgaaagag tgattttgtt
    18841 tcgtgtcccc tggatggatg ataagggaga aattcagatt aatcgcggat atcggataga
    18901 aatgaacagc gccataggac catacaaagg cggacttcgt tttcatccga cggttaattt
    18961 gggaattttg aaattcctcg cttttgaaca ggtgtttaag aatgctctca ccactcttcc
    19021 catgggaggc ggcaaaggag gatcagattt tgatcccaaa ggaaagtctg acaatgaagt
    19081 gatgaagttc tgtcagagtt ttatgcttga actccagaga cacattggtc cagatacaga
    19141 tgtcccggca ggtgatatcg gggtaggtgg tcgggaaata ggatttatgt tcggacagta
    19201 tagaaggatc cgcaacgagt tcaccggagt gctaaccggt aaaggactta attggggagg
    19261 aagcttaatc cgaccagaag ccacgggata tggctgcgtc tattttgtgg aagagatgtt
    19321 aaaaaccaaa ggagattcta ttaaaggtaa gatctgcaca gtttccggtt cgggtaatgt
    19381 cgcccaatat actgtggaaa aggtaaacca actgggcgga aagtgcgtca ccttatccga
    19441 ttccaatgga tatgtctatg acccagcagg tgttgatgcc aaaaaacttg agttcgttat
    19501 tgaacttaaa aatgtcagaa gaggccggat taaggaatat gcggataagt ttggctgcaa
    19561 atatgtggac ggcaagcgcc cttgggaagt aaaatgcgat gtggcattcc cttccgccac
    19621 ccagaacgaa ataaatgaag aagatgccag gatgctggtt aagaacgggt gtaaggctgt
    19681 gggtgaaggt gcgaatatgc caaccacctt tgaagggact caagtgtttc tcaatgctaa
    19741 gatcctattc ggtcccggca aagcggctaa tgccggtggt gtagctactt caggccttga
    19801 gatgtcacag aactcgcaga gattgtcctg gacacgcgaa gaggtggatg caaaactgca
    19861 cggaattatg aaagctattc accagcagtg cgtgaaatac ggaaaggaaa aggactgggt
    19921 gaattatgtg aaaggtgcca acatcgccgg gttcatgaaa gttgcgaatg caatgttgga
    19981 tcaaggcttg gtttaatcaa aggtgcttca agcacaaaag gggtaccagt ttctcaattg
    20041 gtaccctctt tcatttcaga acttgtggct gaatacttcc tcaacttttt gaaggacgaa
    20101 tcttgcggta aatgctttgc gtgtcgagaa aaggcatgga tggaagaata cctttgagaa
    20161 aacttagaga atcctattct catatcaacc cgagcacacg tcttttccca cccttcacct
    20221 tagaggaagt cgctcctctg atttggtctg gacctggtaa cgatgtgtcg gacatgcacc
    20281 aaaacttata agatgtttgc aagttcaatt gtagactaaa aacccaatca gaactttttc
    20341 ctatttttct tgagacattt attttctaac aatcaacttg ctccaacttg aaggaacttg
    20401 cttgtactcc aagtaactcg atgcaactca aatcgagatt ttcatttgca tttgcgggac
    20461 acgatgctat atttgtagat aggatcttat gaaaaagagg ctttcaaata aaattaacac
    20521 ggatatttcg aaccacaagc tgacagagaa tcttcgggct ctatcgcatc aggtgttgta
    20581 ttatgccaat cttggaattc cgagaattga ttttatgcgc gaagtcagca gaatgctgat
    20641 tgacttctct cggtgtgatg tggttgaaat gcgtatcaaa cgaagtgata aatactttct
    20701 ttgcgaagcc gtaagaggca ataaggtttc cttcaggata gaccacctgt cctgtgcgca
    20761 agtcgagaaa gataaaatga taccttgctc acaaagcgat tcgggcatag agactctgtg
    20821 tcataacatt atgctgaaag aattcgactc ttcatctccc ttctttacga aaaatggcag
    20881 tttctggacc agcaatgtgg atgatttact tacgactcag tccaacaatc ttcgtctcga
    20941 tggcgaatat aaatccatag cactttttcc tctggcggtg aacgaagaga atatagggct
    21001 tctgcagctg aagagcaaac agcgagacta cttcactgag aaagagatag aactttacga
    21061 gggttttgcc caaactcttg gggttgcgct ggcgcaccgt catgcgcagg tggcgttacg
    21121 cgaacgaatc aaggaattaa cctgcctcta tggtatagct cggttagccg caaggactga
    21181 tttgtctttg gacgagatat tgcagaaaat tacagaactt cttcctcctg cttggctcta
    21241 tcctgagatt gcctccggca aaatttccct gaatggatat tcctacgcga ccccggactt
    21301 ccgagaaggt ttgcatattc agaaatcgga tatagtggtt aaaggaggta agcgaggatt
    21361 ggttgaggta acctatgcag aggaaaaacc cgagctggat gaaggcccgt tccttaaaga
    21421 agagcggagt ttgatagata ctatagctaa ggatgttgcg ctcattatag aacgcaggca
    21481 ggcggaggaa gaaaaagtaa atcttcagaa ccaactcatg cacgctgatc gattagccac
    21541 cattggacaa cttgctgccg gcgtggctca cgaactaaat gaaccgttaa gcaacatcct
    21601 cggatttgcc caacttgcaa agaaaattcc caaattatcg aaacaaacta ttggggactt
    21661 agaaaagatc gagtctgctt ctttgcacgc tagagaaatc gtcaaaaaac tgatgatatt
    21721 cgctcgacag atgcctccca aaaagacccg ggtcaatcta agccaattag ttgaagaaga
    21781 attatatttt tttgagtcca gatgcgccaa agagggaata gaactgatac gcctgctcgc
    21841 gccaaaattg ccgaagatta ttgcggatgc gggacaattg catcaagttc tggtcaattt
    21901 agtggtcaat tccatccagg caatgccaag aggaggaaag ttaacaattc aaacttcagc
    21961 cggagacaat tatatttcgt taattgtgaa agacaccggc ataggcatga acgaagaggt
    22021 tttaaaacac ttatttgtgc cgtttttcac caccaaagaa gtaggtgagg gcacgggact
    22081 cggactttca gtggttcatg gaataataag ttcacatcaa ggttccatag aggttaaaag
    22141 cgaggtgggt aatggtactt catttgagat tcacctgccc ataaaaactc aaccacatcc
    22201 aaaggagagt atctaagatg gccagctcac ccaagaaaga acgcatatta gttgtagatg
    22261 acaaccccaa tacattggag gtgcttaaca gaaatctgac atcacagggg tatcaggtgt
    22321 taattgcacc gggcgtggcg gaggcaatta aaatattgga tgggacacta gttgatttag
    22381 tcataactga ccttaagatg ccaaaagtca gtggccttga tctgataaga catattagag
    22441 aaaatttcaa ggagactgag gtaatgatga ttaccggcta cgcctcgata caaggagcag
    22501 ttaaggcggt caagaccgga gcggaagatt atctggccaa accttttact gacgaggaac
    22561 tcttttctgc tgtgcgcagg tcactggata agttacattc acgcagaacc agacaggatc
    22621 aaactcacca tatatcgaca acctcttatg gtctgattgg tgaatcggaa gcaatgcaca
    22681 gagtgcttgg tgctataact aaggcagcat ccacttcagc taccgtgctt atcaccggag
    22741 aaagtgggac aggcaaggag cttgttgcta gagcaattca ttataacagc tccagagttt
    22801 catcttcttt tgtgccggtg aattgcgggg gtataccgga aacgctgtta gaaagcgagc
    22861 tttttggtca tgttaagggc tcttttactg gagccaccga atcgagagcg ggattcttcc
    22921 agactgccga tggaggaacc atattcttgg acgaaatcag cgaaactagt ttatctatgc
    22981 aagtaaaatt tcttcgcgtg ttgcaggaca aagaagtcta tatggttgga gccacccgtc
    23041 cgagaaaagt aaatgtgaga atcatagccg caaccaataa ggatttgcgc aatctggtga
    23101 aaaaagaggt tttccgggag gaccttttct ttcgtctgaa cgtgataacc attgctgtcc
    23161 cttctctgcg tgacagagaa gatgatattc tcttgttgat tgatcatttt gcatctaaat
    23221 ttggaaagga atttgataag ccagtgctcc gattctccga cgaagctttg caggtcctca
    23281 agaattacga ttggcctgga aatgtgagag aattggaaaa catggtgcaa cgtctggtag
    23341 ttatgaccga tagttatatg attgacgttc cagatctacc ttcgcctatg cgtttttctg
    23401 ctctgcgaga tgtggggctt aataggactt tagctgaagt ggaagcagag tatattcgca
    23461 atgtcctggc gactgttgat ggtaacaaaa ctaaagcttc gcacatactc gatatagacc
    23521 gaaagacatt gcgggaaaaa ttacagaaaa caaagctttc gaataaacca tagcccctat
    23581 tgtcattggg gaaagtctcc ccaatggttc aaattacccc atcaattcta cttttctact
    23641 ctgaatataa taagaaagat tatcctttta atctatttcc tatcttggac ttaactaata
    23701 cctccttgtt cagcattgat tcttaatctt tggcacagag attgctttat gcttcaatcg
    23761 aaaaaacaat aaaagaaaaa ggaggtaagt aaaatgactg tctatcaagc catcaaaaaa
    23821 gtacaaaaaa aggaagctct ctctaaaaga gaacagaaaa aagaaactct ctctaaaaaa
    23881 ggagagtatg ctggcctttg cctaacttgt gtgaatgcgt ccggttgtat ctacattaag
    23941 aatcagaaat ccgtagtttt ggagtgcgaa gatttcgaag gatatacaaa ctctccaatg
    24001 agagccacaa taaatgatgt gctttcaaaa gtaagttcag atgcttcaaa agcggatttc
    24061 ggaaaatacg aaggattatg cgtgaattgc gagattcgtg aaacctgcga gtttcctaaa
    24121 tctgaaggcg gcgtttggca ctgtgaagaa tatcaataag acctgggcgt ataattataa
    24181 tttaagcaac gaaaaagaag tggaaggagg caactgatgg caaaggcatc cgtggattct
    24241 gcatcttcgg gaaaaaaggg gagcgtctat gataatgtaa gctggcaatt caataaggcc
    24301 gccgacctta tgaagctcga ccagaacatt cgtcatatct tggctaaaac caccaatgaa
    24361 atagtggtca attttccggt cagaatggat gatgaacgaa ttgagatgtt taccgggtat
    24421 cgggtgcaac acaataatgc tctcggacct ttcaagggag gactaagata ccatcc
//
LOCUS       DASWLN010000103         2943 bp    DNA     linear   ENV 20-FEB-2024
DEFINITION  MAG TPA_asm: Candidatus Zixiibacteriota bacterium isolate
            SMAG_U2949 k141_803478, whole genome shotgun sequence.
ACCESSION   DASWLN010000103 DASWLN010000000
VERSION     DASWLN010000103.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37960594
            Sequence Read Archive: SRR10376091
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Candidatus Zixiibacteriota bacterium (soil metagenome)
  ORGANISM  Candidatus Zixiibacteriota bacterium
            Bacteria.
REFERENCE   1  (bases 1 to 2943)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 2943)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (26-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/12/2024 08:28:26
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 1,440
            CDSs (total)                      :: 1,416
            Genes (coding)                    :: 1,412
            CDSs (with protein)               :: 1,412
            Genes (RNA)                       :: 24
            rRNAs                             :: 1 (16S)
            partial rRNAs                     :: 1 (16S)
            tRNAs                             :: 21
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 4
            CDSs (without protein)            :: 4
            Pseudo Genes (ambiguous residues) :: 0 of 4
            Pseudo Genes (frameshifted)       :: 0 of 4
            Pseudo Genes (incomplete)         :: 3 of 4
            Pseudo Genes (internal stop)      :: 1 of 4
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2943
                     /organism="Candidatus Zixiibacteriota bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_803478"
                     /isolate="SMAG_U2949"
                     /isolation_source="soil"
                     /db_xref="taxon:2053527"
                     /environmental_sample
                     /country="USA: Oregon"
                     /lat_lon="42.99 N 123.63 W"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            complement(<1..753)
                     /locus_tag="VGB16_03515"
     CDS             complement(<1..753)
                     /locus_tag="VGB16_03515"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_006928932.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_process: GO:0043952 - protein transport by the Sec
                     complex [Evidence IEA];
                     GO_process: GO:0065002 - intracellular protein
                     transmembrane transport [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="preprotein translocase subunit SecY"
                     /protein_id="HEX9916782.1"
                     /translation="MIATFQNMFRIPELRKRILFTLGILVVYRVGCHVPTPGVDPSVL
                     VSWFERAGKGSLFGLYNLFAGGAFAKATIFSLGIMPYISASIIMQLLGAVIPYFQKLQ
                     KEGEEGRRKITQYTRYGTVLLSAMQALGVSAFLESIKEGGVSAVARPGFIEFTLPTII
                     TFSCGTVLIMWLGEQITERGIGNGISLIIFIGIVARFPSAIIDEFNNIRLGERNILTE
                     VVMIAFMVLVIAAVVAVTAALRKIPVQYAKRIV"
     gene            complement(750..1196)
                     /gene="rplO"
                     /locus_tag="VGB16_03520"
     CDS             complement(750..1196)
                     /gene="rplO"
                     /locus_tag="VGB16_03520"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011026320.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0000315 - organellar large ribosomal
                     subunit [Evidence IEA];
                     GO_component: GO:0022625 - cytosolic large ribosomal
                     subunit [Evidence IEA];
                     GO_function: GO:0003735 - structural constituent of
                     ribosome [Evidence IEA];
                     GO_process: GO:0006412 - translation [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="50S ribosomal protein L15"
                     /protein_id="HEX9916783.1"
                     /translation="MKLGELRKPFGSTKKNKRIGRGPGSGHGKTSTRGHKGQKARAGS
                     VARRAWSEGGQMPLQRRVPKRGFTNIFKKEYQIVNLGSLEELAGEGIISPETLKAKGL
                     TKKSNVPVKILAGGEITKGITIRAHFFSKKAQEKITSLGGKAEVIK"
     gene            complement(1199..1387)
                     /gene="rpmD"
                     /locus_tag="VGB16_03525"
     CDS             complement(1199..1387)
                     /gene="rpmD"
                     /locus_tag="VGB16_03525"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_004081798.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0000315 - organellar large ribosomal
                     subunit [Evidence IEA];
                     GO_component: GO:0022625 - cytosolic large ribosomal
                     subunit [Evidence IEA];
                     GO_function: GO:0003735 - structural constituent of
                     ribosome [Evidence IEA];
                     GO_process: GO:0006412 - translation [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="50S ribosomal protein L30"
                     /protein_id="HEX9916784.1"
                     /translation="MPKKLKITQIRSVSGRLDKQISTLSALGLRRIRQIVIKNDTPQI
                     RGMIEKVRHLVKVEETEE"
     gene            complement(1356..1868)
                     /gene="rpsE"
                     /locus_tag="VGB16_03530"
     CDS             complement(1356..1868)
                     /gene="rpsE"
                     /locus_tag="VGB16_03530"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_020267830.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0000312 - plastid small ribosomal subunit
                     [Evidence IEA];
                     GO_component: GO:0022627 - cytosolic small ribosomal
                     subunit [Evidence IEA];
                     GO_function: GO:0003735 - structural constituent of
                     ribosome [Evidence IEA];
                     GO_process: GO:0006412 - translation [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="30S ribosomal protein S5"
                     /protein_id="HEX9916785.1"
                     /translation="MFKSKEESLEYVEKVIHVSRVAKVVKGGKRFSFTALVTVGDRNG
                     KVGVGLGKAGEVVDAINKGTEVAKKNMVEIPLREGTIPHSVTGRFGAAVVLLRPASPG
                     TGVIAGGPVRAVLEAAGVQDVLTKSLGSPNPHNVVKATLNGLLSIKEYEELNKERSAS
                     YAKEIKDNTD"
     gene            complement(1902..2276)
                     /gene="rplR"
                     /locus_tag="VGB16_03535"
     CDS             complement(1902..2276)
                     /gene="rplR"
                     /locus_tag="VGB16_03535"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_012499652.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0000315 - organellar large ribosomal
                     subunit [Evidence IEA];
                     GO_component: GO:0022625 - cytosolic large ribosomal
                     subunit [Evidence IEA];
                     GO_function: GO:0003735 - structural constituent of
                     ribosome [Evidence IEA];
                     GO_process: GO:0006412 - translation [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="50S ribosomal protein L18"
                     /protein_id="HEX9916786.1"
                     /translation="MADKMKFKKLSWERRQRRVRKKVKGTEMRPRLCIYKSLRQIYAQ
                     VIDDVSGRTILGTSSLNPEVKKLLGEKDKKTELGKKVGLYLAQLAKAKGIEEVVFDRN
                     RYRYHGRVKALAEGAREGGLKF"
     gene            complement(2457..>2943)
                     /gene="rplF"
                     /locus_tag="VGB16_03540"
     CDS             complement(2457..>2943)
                     /gene="rplF"
                     /locus_tag="VGB16_03540"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_006928927.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0022625 - cytosolic large ribosomal
                     subunit [Evidence IEA];
                     GO_function: GO:0003735 - structural constituent of
                     ribosome [Evidence IEA];
                     GO_function: GO:0019843 - rRNA binding [Evidence IEA];
                     GO_process: GO:0006412 - translation [Evidence IEA]"
                     /codon_start=2
                     /transl_table=11
                     /product="50S ribosomal protein L6"
                     /protein_id="HEX9916787.1"
                     /translation="KGDEVTVQGPKGKLSKRLHPSIRLEQKEGKLVVTRTSDSKFHKS
                     LHGLSRALVANMVKGVTQGFQKMLEIQGIGYKAEMKGKDMILNLGFSHPILLSPPPSI
                     ELKLDDPTKIVVSGSDKELVGLVAAKIRSFYPPEPYQGKGIRYRGERVRRKAGKTAAA
                     A"
BASE COUNT          727 a          797 c          514 g          905 t
ORIGIN      
        1 tacaatcctt ttagcatatt gaaccggaat tttgcgcaac gcagcagtga ccgccacaac
       61 cgcggcaatc actaacacca taaatgctat catcaccact tcagtgagaa tgttcctctc
      121 tcctaagcgg atattgttaa attcatctat gattgcactt gggaaacgag ccacaatccc
      181 gatgaagata ataagtgaaa tcccatttcc aattcctctc tccgtaattt gttcacccag
      241 ccacataatc aggacagtcc cacaggagaa agtgattata gtcgggagcg taaattctat
      301 gaaccctggt ctggccacag cacttacgcc accttccttg atgctttcca aaaaagcgct
      361 aacgcctaaa gcttgcatag ctgaaagaag gactgttcca taacgggtat attgggtgat
      421 ctttcttctt ccttcttctc cttccttctg tagtttctga aaataaggaa tcaccgctcc
      481 taaaagctgc atgataattg aagcggagat gtaaggcata attcccaagg aaaagatggt
      541 cgccttggca aaagctccgc ccgcaaaaag attgtaaaga ccaaaaagag aacccttgcc
      601 tgctctttcg aaccaactca ccaaaactga aggatcaact cccggagtgg gaacgtgaca
      661 acccacacga tagaccacca aaatacccag ggtgaaaagg atgcgcttgc gcaactccgg
      721 aatcctgaac atattctgga aagttgcaat cactttatga cctcagcctt tccacctaaa
      781 gaggttatct tctcttgtgc ttttttgctg aaaaagtgag cccgtatggt aattccttta
      841 gttatctccc ctcctgccag tatcttcacc ggaacattgg atttttttgt caaacccttg
      901 gctttcagag tttccggact aattattcct tcacccgcca attcttctaa tgaacccaag
      961 tttacaatct gatattcttt cttaaagata ttagtgaacc ctcttttagg aactctcctc
     1021 tgtagaggca tctgtccacc ctcggaccaa gctcttctcg ctacagaacc agctctagcc
     1081 ttttgtcctt tatgaccacg agttgaggtc ttgccgtgac ctgagcctgg tcctcgacct
     1141 atcctcttat ttttcttagt ggaaccaaac ggttttctca attcacccaa tttcatttct
     1201 actcttcagt ctcctctact ttgactaaat gccttacttt ttctatcatt cccctgattt
     1261 gaggggtatc gttttttata actatctgac gaatccttct tagtcccaga gcagacaagg
     1321 tgcttatctg cttgtctaat ctaccagata cacttctaat ctgtgttatc tttaatttct
     1381 ttggcataac ttgcacttct ttctttattt aactcctcgt attcttttat gctgagtaga
     1441 ccatttagag ttgctttaac tacgttatgt ggattcggcg agcccaaaga cttggtcagc
     1501 acatcttgaa ctccagctgc ttccaatacc gccctcaccg gcccaccggc aatcaccccg
     1561 gttccaggtg aagctggtct gagcaggacc actgccgccc caaatcttcc agtgaccgaa
     1621 tgtgggatag tgccttccct taggggtatc tctaccatat ttttcttggc tacctcagtc
     1681 cctttattga tggcatccac tacctcacct gccttgccca aacccactcc cacttttccg
     1741 tttctatcgc ccactgtaac taatgcggtg aagctaaatc tctttccacc tttgaccact
     1801 ttagctacgc ggctaacatg aattaccttc tcaacgtatt ctaaggattc ctctttactt
     1861 ttaaacaagt ttaacctctt cttttttgaa ttcgaccctt cctaaaactt aagtccccct
     1921 tctctggctc cctctgccaa agcctttact ctgccgtgat acctatatct gttgcgatca
     1981 aagaccacct cttcaatccc tttagcttta gccagttggg ctaagtagag acccaccttt
     2041 ttccccagct cggtcttctt gtctttttct cctaacaatt tcttgacttc agggtttaag
     2101 ctggaagtac ccaggatggt gcgaccgctt acgtcatcta tgacctgtgc gtatatctgc
     2161 cttaagcttt tataaatgca cagccgggga cgcatttctg ttcccttgac tttttttctg
     2221 acccttctct gtcgacgctc ccaactcaac ttcttgaatt tcattttatc agccataatg
     2281 ctttcctact aatacaaacg cattaattta cgttcaataa atctcccctt gcccctcttt
     2341 ttcaaagagg ggaaaaaaga ggcctccccc tttgaaaaag agggatttga gggggatttt
     2401 aaaatagatt gcatattgtt ctaatatgta acaacattta atccttttgc tatactctaa
     2461 gctgcagcag cagtcttgcc agcttttctc ctaactcttt cgccacggta cctgattccc
     2521 ttgccctgat aaggttcagg cgggtaaaag gaacgaatct tagccgccac caaaccaacc
     2581 aactctttat ccgagccaga taccacgatt ttagtggggt catcgagctt aagctctatg
     2641 cttggcggag gagagagaag tatcggatga gagaagccca aattcaaaat catatctttt
     2701 cccttcatct ccgctttgta gccgataccc tgaatctcca gcatcttctg aaacccctgg
     2761 gtcacacctt tcaccatatt agccaccagc gctcgggata ggccgtgtaa ggacttatgg
     2821 aatttgctgt cagaggtgcg agttactacc agctttcctt ccttctgttc caatcttata
     2881 ctgggatgga gccttttact taattttccc tttggtccct ggacagtaac ctcatcccct
     2941 ttt
//
LOCUS       DASWLN010000104         3049 bp    DNA     linear   ENV 20-FEB-2024
DEFINITION  MAG TPA_asm: Candidatus Zixiibacteriota bacterium isolate
            SMAG_U2949 k141_729203, whole genome shotgun sequence.
ACCESSION   DASWLN010000104 DASWLN010000000
VERSION     DASWLN010000104.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37960594
            Sequence Read Archive: SRR10376091
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Candidatus Zixiibacteriota bacterium (soil metagenome)
  ORGANISM  Candidatus Zixiibacteriota bacterium
            Bacteria.
REFERENCE   1  (bases 1 to 3049)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 3049)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (26-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/12/2024 08:28:26
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 1,440
            CDSs (total)                      :: 1,416
            Genes (coding)                    :: 1,412
            CDSs (with protein)               :: 1,412
            Genes (RNA)                       :: 24
            rRNAs                             :: 1 (16S)
            partial rRNAs                     :: 1 (16S)
            tRNAs                             :: 21
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 4
            CDSs (without protein)            :: 4
            Pseudo Genes (ambiguous residues) :: 0 of 4
            Pseudo Genes (frameshifted)       :: 0 of 4
            Pseudo Genes (incomplete)         :: 3 of 4
            Pseudo Genes (internal stop)      :: 1 of 4
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..3049
                     /organism="Candidatus Zixiibacteriota bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_729203"
                     /isolate="SMAG_U2949"
                     /isolation_source="soil"
                     /db_xref="taxon:2053527"
                     /environmental_sample
                     /country="USA: Oregon"
                     /lat_lon="42.99 N 123.63 W"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            204..992
                     /locus_tag="VGB16_03545"
     CDS             204..992
                     /locus_tag="VGB16_03545"
                     /inference="COORDINATES: protein motif:HMM:NF019342.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="gamma-glutamyl-gamma-aminobutyrate hydrolase
                     family protein"
                     /protein_id="HEX9916788.1"
                     /translation="MPRELGNYNETVLSFERIIMKKPVIGITCSMRKEDKKREFPTAY
                     AFEYLNRPYSKAVEKAGGLPILLPNLENPKLIENVLSLTNGLLVSGGGDVSPELYNQE
                     VKAKLISFARERDEFEMELIQQAEKRKLPILGICRGMQVINVAFGGSLYQDFKLNPVY
                     SDHRRRGNLYLRKHEIRIEKNSLLYSIMRKDELTVNTSHHQMIDLLSPEFEISATSVK
                     DGVVEAIEHKTKPILGVQWHPEVMGDLYSKKIFKWLINQAMKYE"
     gene            985..1614
                     /gene="thiE"
                     /locus_tag="VGB16_03550"
     CDS             985..1614
                     /gene="thiE"
                     /locus_tag="VGB16_03550"
                     /EC_number="2.5.1.3"
                     /inference="COORDINATES: protein motif:HMM:TIGR00693.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004789 - thiamine-phosphate
                     diphosphorylase activity [Evidence IEA];
                     GO_process: GO:0009228 - thiamine biosynthetic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="thiamine phosphate synthase"
                     /protein_id="HEX9916789.1"
                     /translation="MNKKDILSKAKLYLIVDADLLEDEDLEKLTSGAIEGGADLIQFR
                     GKTLTDGEFLKTAESLKKVTDHRNIPLIINDRVEIALYLNTAGVHLGQEDLLISVTRK
                     LLGEEKIIGISASNLKEAKNAQTDGADYLGVGPVFYTRTKKIIKTVGLELIREIKREI
                     TIPFFAIGGINLENMDEVRESGADKIAVASAVLKAKDPKLAARKLRDRL"
     gene            2031..2471
                     /locus_tag="VGB16_03555"
     CDS             2031..2471
                     /locus_tag="VGB16_03555"
                     /inference="COORDINATES: protein motif:HMM:TIGR00738.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003677 - DNA binding [Evidence IEA];
                     GO_function: GO:0003700 - DNA-binding transcription factor
                     activity [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="Rrf2 family transcriptional regulator"
                     /protein_id="HEX9916790.1"
                     /translation="MSISTRGRYGLRALLEIAQNYGSGPINIRDISKNQKISMSYLEQ
                     ILHRLKKAGLIRSIRGAKGGYLLAKESNQITIKDIVKVLDGPIGVAYCDFPNLREKSC
                     IGPKVCASSILWKRLELTIDDFLSKVTLADLKEEPPSSKKGMRK"
     gene            2468..>3049
                     /locus_tag="VGB16_03560"
     CDS             2468..>3049
                     /locus_tag="VGB16_03560"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_007290595.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0030170 - pyridoxal phosphate binding
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="cysteine desulfurase family protein"
                     /protein_id="HEX9916791.1"
                     /translation="MRKIYLDHSATTPLHPEVLEAMLPYFREHFGNASSTHGWGREAK
                     VILEECRERIAGYFGTSSKEIFFTSGGTESDNLAIKGVAWANENKGKHIVISKIEHHA
                     VFESCKYLSKQGYEITYLPVNEYGLVDPEDVKNAIKPDTILVSIMHVNNEVGVIEPIG
                     EIGNIAKEKGIYFHTDAVQSAGKLPIDVNKQNLD"
BASE COUNT          994 a          533 c          741 g          781 t
ORIGIN      
        1 actccgaggt cactcagact cgaggagagc tcagggtcga agactgaggg acagtgagcg
       61 aagtcgaact gtcaccatcc tgagcttgtg cttcgacttc gctcagcacc atgagtttat
      121 cgaatggtcg aaggatcgag cgatcttaaa cctcacccgt tccctctcct tattaaggag
      181 agggctgggg agagataaaa aatgtgccca gggaattggg caactacaat gaaacagttt
      241 taagcttcga gaggataatt atgaaaaaac cggtcatcgg aatcacctgc tcaatgcgaa
      301 aggaggataa gaaaagagaa tttcccacag cttatgcttt tgaatatctc aatcgacctt
      361 attccaaagc agttgagaaa gctggtgggc tgcctattct cttgcccaat cttgagaatc
      421 cgaaactgat tgagaatgtc ctctctttaa caaatggttt gctggtgagt ggtgggggtg
      481 atgtctcgcc cgaattatat aatcaggaag tgaaggcaaa acttatttct ttcgcacgag
      541 agagagatga atttgaaatg gagttaatcc aacaggcgga aaaaaggaag ttgcccatct
      601 tgggcatatg tcggggaatg caagttataa atgtcgcctt tggaggaagt ttgtatcagg
      661 atttcaagct gaatccagtt tattcagacc atcgaagaag gggaaacttg tatttgagaa
      721 agcacgaaat cagaatagag aagaatagtt tactttattc gataatgcgg aaagatgaac
      781 ttacagtcaa caccagccat caccagatga tagaccttct ttctccggag tttgaaatat
      841 ctgctacctc agtcaaagat ggggtggtcg aagctattga acataaaact aaaccgattt
      901 taggggtgca gtggcatcca gaggtaatgg gggatttata ttcaaagaag atttttaaat
      961 ggctaattaa ccaagcgatg aaatatgaat aagaaggaca ttttatcaaa agctaagctt
     1021 tatttgatag tggacgcgga tttgttggag gatgaagatt tagaaaaatt aacctcaggg
     1081 gcaattgagg gtggggcgga tttaattcaa ttcaggggca aaaccttaac tgatggcgag
     1141 tttctaaaaa cagctgaatc attgaagaaa gtaacagacc atagaaatat acctttaatc
     1201 ataaacgaca gggtggagat agcgttatat ctgaatacag ctggggtgca tctgggtcag
     1261 gaggatttgc ttatttcagt tacaagaaag cttctgggag aggagaaaat aatcggcata
     1321 tccgcttcga atttaaaaga ggcaaaaaat gcgcaaacag atggggcaga ttacctcgga
     1381 gttgggccgg tgttttatac ccgaaccaag aagataataa agacagttgg tttagagcta
     1441 atacgagaga ttaaaaggga aatcactatt cccttttttg caattggcgg gataaatctg
     1501 gagaacatgg atgaggttag agaatcaggg gcggataaaa tcgcagtggc ttcggcggtg
     1561 ctgaaagcca aagacccgaa attagccgca agaaaattga gagatagatt atagtttttt
     1621 gcagattatg tcaggagtag ctcctgacat aatctgaaac tacaaattat gacaacctca
     1681 cctttatcct ctccttggca tgaaccataa tagttcacgc cttggaaagg agaggaaatc
     1741 ttaaatggcc tcactcgtac cctctcctta tttaaggaga gggttaggga gaggttaatg
     1801 cagtagtccc tacattaaag caagattgct tcgtcatttc gataaaatta ggactcctcg
     1861 gtatgaaccg ttctggttca tacctcgcaa tgacaaattc ctacttactg ggataaatta
     1921 gagaatcata aagagcaaac tgccttatgc cgattgtttt caaataaatc ttgacaaaaa
     1981 aagtaaagat tttatatttt ttgtgcgtat aaataaaaaa ggaaacaatt atgagtattt
     2041 caactcgtgg tcgctatggt ttaagggcgc tattggagat tgcgcagaac tacgggagtg
     2101 gaccaattaa cattcgggat atatccaaaa accagaagat atcaatgagc tacttggagc
     2161 aaattctgca tcgcttaaag aaagccgggt taataagaag catcagagga gctaagggtg
     2221 ggtatctctt agccaaagag agcaaccaga taacgattaa agacattgtc aaagtcttgg
     2281 acggtcctat aggtgtagct tactgtgatt tcccaaactt gagggaaaaa agctgcatcg
     2341 ggccaaaagt ttgcgcctct agtatactat ggaaaagatt agagctgacc atagatgact
     2401 ttctttccaa agtaacctta gcggatttaa aagaggagcc tcccagctca aaaaaaggta
     2461 tgagaaaatg agaaagatat atcttgacca tagcgctacc actccacttc atcctgaagt
     2521 cttggaggcg atgcttccct atttcagaga acatttcggc aacgcctcca gcacccacgg
     2581 ttggggacgg gaggctaaag taatacttga ggagtgtcgg gaaagaatag ccggttactt
     2641 tgggacgtcg tccaaggaga tattttttac cagcggtggt actgaatcag ataacttggc
     2701 gattaaggga gtagcttggg caaacgagaa caagggaaaa catatcgtta tctccaaaat
     2761 tgagcatcat gcagtcttcg agagttgcaa atatctttcc aagcagggat atgaaatcac
     2821 ctatttgccg gtaaatgagt atggtctggt tgacccggag gatgtaaaaa atgcaattaa
     2881 gccagatact attctggtct caattatgca tgtgaacaat gaagtaggag taatagagcc
     2941 gataggggag atagggaata tagccaaaga gaaagggata tattttcata cggatgcagt
     3001 gcagtctgct gggaagttgc ccattgatgt taataagcaa aatctggat
//
LOCUS       DASWLN010000105        11826 bp    DNA     linear   ENV 20-FEB-2024
DEFINITION  MAG TPA_asm: Candidatus Zixiibacteriota bacterium isolate
            SMAG_U2949 k141_89796, whole genome shotgun sequence.
ACCESSION   DASWLN010000105 DASWLN010000000
VERSION     DASWLN010000105.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37960594
            Sequence Read Archive: SRR10376091
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Candidatus Zixiibacteriota bacterium (soil metagenome)
  ORGANISM  Candidatus Zixiibacteriota bacterium
            Bacteria.
REFERENCE   1  (bases 1 to 11826)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 11826)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (26-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/12/2024 08:28:26
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 1,440
            CDSs (total)                      :: 1,416
            Genes (coding)                    :: 1,412
            CDSs (with protein)               :: 1,412
            Genes (RNA)                       :: 24
            rRNAs                             :: 1 (16S)
            partial rRNAs                     :: 1 (16S)
            tRNAs                             :: 21
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 4
            CDSs (without protein)            :: 4
            Pseudo Genes (ambiguous residues) :: 0 of 4
            Pseudo Genes (frameshifted)       :: 0 of 4
            Pseudo Genes (incomplete)         :: 3 of 4
            Pseudo Genes (internal stop)      :: 1 of 4
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..11826
                     /organism="Candidatus Zixiibacteriota bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_89796"
                     /isolate="SMAG_U2949"
                     /isolation_source="soil"
                     /db_xref="taxon:2053527"
                     /environmental_sample
                     /country="USA: Oregon"
                     /lat_lon="42.99 N 123.63 W"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            complement(<1..265)
                     /locus_tag="VGB16_03565"
     CDS             complement(<1..265)
                     /locus_tag="VGB16_03565"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_020877008.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEX9916792.1"
                     /translation="MRAWVLFLTGAILFALVSCKEEEMDKDKSPEDIYLELREKMVVE
                     QIESRGVHNKLVLDATRKVPRHLFVPSAYMDMAYDDGPLPIGED"
     gene            complement(308..2458)
                     /locus_tag="VGB16_03570"
     CDS             complement(308..2458)
                     /locus_tag="VGB16_03570"
                     /inference="COORDINATES: protein motif:HMM:NF025141.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="VIT and VWA domain-containing protein"
                     /protein_id="HEX9916793.1"
                     /translation="MKRLILIVASLVGLLVSSAWADGFIIIQPDPRQRILPPPLSVLY
                     HQVDVRITNQLCQTSIDQVFVNNHHQDLEGIYIFPIPKGASITDFSMFVGERELKGEI
                     LDKGQARKIYEEIVRRKKDPALLEYYDQGMFRARVYPIPAKGRVRIRIKYSEILKKEE
                     GILSYTYLLSTEKFSQDPLERAGINIDLSSDLPIETIYSPTHDIKIEKLGNKQARITY
                     TERGTKPDKDFKLFYTVSRDEIGFHILTYGNFFLGLLSPQADISSQKVVGKNIVLILD
                     SSGSMSGDKIRQAKDALVFCLNNLNPQDKFNLIDFDDRIRSLRSSLVPATAQNIKEGI
                     DFISLCSADGGTNINDALLEGLNQIKNSSSLSMIVFLTDGLPTVGQTDIKTILQNISQ
                     ENRTSTRLFVFGLGYDVNTNFLDNLAEQNKGLSDYIKPGEDIEVKVSSFYRKVANPIL
                     SDVQLEFTGVEVYDIYPSRLPDIFKGTQLVILGKFKGDHKAGVVLTGKVSGMPKTFRY
                     EADFEKKSDADFVPKLWATRRIGYLIDQIRLHGENREIIDEIVRLSREYGIITEYTSY
                     LIDADSHLSHSQSVEYTAQRMADLKEEVGSLAVNQALGVAKMKGSRAPADAYIDSQGK
                     LNRISQVITSEGKTFFNRNGIWIDASYDGNRPTLKIKKFSQAYFKLLNNLPGLGKYFS
                     LGQEIIICLDGIVLEIANQGEDNLTEDELKKIRY"
     gene            complement(2542..3630)
                     /locus_tag="VGB16_03575"
     CDS             complement(2542..3630)
                     /locus_tag="VGB16_03575"
                     /inference="COORDINATES: protein motif:HMM:NF024881.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="zf-HC2 domain-containing protein"
                     /protein_id="HEX9916794.1"
                     /translation="MRCDQIEKLLYLYEDGELDEKEKREVGEHIKICPNCSKKLADLK
                     LIRAEVKEKKVPQPSETYWESFAHRVKERIEDKTKVSFGQRFSYFLEMIFSYSPTKVK
                     WAAAVVSVILVFVVVKLFVSYEKINIATLGQKAQVQKISPPAGETLGVETNIPREQRK
                     PAIAERGGVAPEREVSKTIQAPLKDMTVSKGLESVAPGEERGVQKETPIADRIAPDKG
                     ETTLKKLPTTTPELKEKEERHTGVQALETPISGTPVMTSAESSKSKATMAAVRGDTVK
                     QEELVRESIDKWRRFIEDKPKGEEKDKAYLNMAQGYMRLYDLARNKDETLKEAMDSVK
                     VYKDSAVTNTYKDSINHILIQLESFHKN"
     gene            complement(3631..4209)
                     /locus_tag="VGB16_03580"
     CDS             complement(3631..4209)
                     /locus_tag="VGB16_03580"
                     /inference="COORDINATES: protein motif:HMM:TIGR02937.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003677 - DNA binding [Evidence IEA];
                     GO_function: GO:0003700 - DNA-binding transcription factor
                     activity [Evidence IEA];
                     GO_function: GO:0016987 - sigma factor activity [Evidence
                     IEA];
                     GO_process: GO:0006352 - DNA-templated transcription
                     initiation [Evidence IEA];
                     GO_process: GO:0006355 - regulation of DNA-templated
                     transcription [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="sigma-70 family RNA polymerase sigma factor"
                     /protein_id="HEX9916795.1"
                     /translation="MSASQSSPLEDQLIREAKGGNYSSFEKLIKKYERVIYGLAYKLV
                     KSHDAADEIAQQTFVRAFYGLKSFHLGCSFYSWIYRICMNLGLNYIKRESLTVSEGAF
                     EQNKSPIDFAVEKDNPETELLKKELSQKIDKEINSLPSKLKAVWILRELEDQSYEQIA
                     QTLKISKGTVMSRLFRARKRLVQNLKGYLRGD"
     gene            complement(4211..5257)
                     /gene="holA"
                     /locus_tag="VGB16_03585"
     CDS             complement(4211..5257)
                     /gene="holA"
                     /locus_tag="VGB16_03585"
                     /EC_number="2.7.7.7"
                     /inference="COORDINATES: protein motif:HMM:TIGR01128.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0009360 - DNA polymerase III complex
                     [Evidence IEA];
                     GO_function: GO:0003887 - DNA-directed DNA polymerase
                     activity [Evidence IEA];
                     GO_process: GO:0006260 - DNA replication [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="DNA polymerase III subunit delta"
                     /protein_id="HEX9916796.1"
                     /translation="MSCIKMNYSDLIKSFERKKIEPTYHFTGEEEFLKNEAISKLIKL
                     LVPDKLKDFNFNLLYASQVEIDQIINSCSTFPLNNPKRVVILYELEKLSPKGKEALLG
                     FLPAVPKTTCLVLVSAKVKMDNKFYKELNQIATTVEFNPLYDNQIPDWIITRVKSYKM
                     KLDQNALELLQNSVGNSLPNLANEIEKLYSFLGKRERITLEDVKTVVGQSKTTNVFEL
                     ANSVGWKDNKKSFSILGKLLLAGERPGTIIYWLTQHFIRLVKTKGFSEKKSGVPLSTY
                     LGIRPFFVRDYKSQVQNFTWKELEQALLFLHQADLEIKSNRLPNKLAMELLVYQLCNL
                     KGGIFNDQNCITKG"
     gene            5765..7042
                     /gene="hflX"
                     /locus_tag="VGB16_03590"
     CDS             5765..7042
                     /gene="hflX"
                     /locus_tag="VGB16_03590"
                     /inference="COORDINATES: protein motif:HMM:TIGR03156.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005525 - GTP binding [Evidence IEA];
                     GO_process: GO:0008150 - biological_process [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="GTPase HflX"
                     /protein_id="HEX9916797.1"
                     /translation="MYQLHKLRWQEKAILVGVKLPQVNRFAAEDSLAELSLLAKTAGA
                     EVLESIIQDRKKIDAGLFIGKGKVEQIKQLCLDRGANLVIFDDDLSPGQIFNLEKILD
                     LKVIDRSCLVLDIFAKRAKTAEAKIQVELAQLQYLLPRLTRGWLHLSRQWGGIGTKGP
                     GETQLEVDRRVIKKKIGDLKGKLVKIDKERQTQRKKREDFFKVALVGYTNVGKSTLFN
                     QLTKATVPVEEKLFSTLDSFTRILRFSRNHNILLTDTVGFIRKLPHHLVASFRSTLDE
                     VKLADLLLHIVDISHPNFPQQMEKVSQILKELDCSSKPTLLVFNKIDKLKGDLPLGLC
                     PSYENEPIFISAEKGIGLANLIASLELFMDEQSCEAEVFLKKEDLNLLPSFYKLGIVT
                     QTEMKDEGLNLKIRAKKWNLEKLKGLANGEDFA"
     gene            7108..7473
                     /locus_tag="VGB16_03595"
     CDS             7108..7473
                     /locus_tag="VGB16_03595"
                     /inference="COORDINATES: protein motif:HMM:NF018228.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0000156 - phosphorelay response regulator
                     activity [Evidence IEA];
                     GO_process: GO:0000160 - phosphorelay signal transduction
                     system [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="response regulator"
                     /protein_id="HEX9916798.1"
                     /translation="MPKDKKILIVDDNPNMSNLLSDILEIFECQSVCAPDGEEALKCL
                     KSQKFDMVITDLRMPKMHGIDLLKTVKENYPGLPVVVITGFSGDTTMDEAIDAHADGF
                     LAKPFRVNDIQKLLERLLG"
     gene            complement(7560..10661)
                     /locus_tag="VGB16_03600"
     CDS             complement(7560..10661)
                     /locus_tag="VGB16_03600"
                     /inference="COORDINATES: protein motif:HMM:NF012622.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="dockerin type I repeat-containing protein"
                     /protein_id="HEX9916799.1"
                     /translation="MIKGIIIFTISLLLVLSLSVFGANKPADYKMIQKLESLDTICYT
                     AEIPPIKYPRIPESKIKYLFSDDTVGYTYYDYQFNAPQHRQIASDLSGTLHFTWMDCI
                     SPDYYYGIRYIDYNARYSNGTWLNPGGVHITPDLVRGGYGGLDILPDNREVLCYHRPY
                     GDDWGSTVSIEDSIPGSGQFNHFDIPDSVAHTSDKSEWPAIGCSKLKNGDTTYFHITH
                     ERSSTGCVTNKELGYVRCFEMPTNRDTLICQSPGWDSPLLIPKDTKLVPNKVPFMYAI
                     AKLGSGVIATSPVSQKVCIAWLQYTSNSATKNEVMYLESTNNGNDWMAAGNMGTPVQI
                     TSYGASGFRDRAYCDIAAVYDYNDELHIMWTTFTENNSNDVTLWHWSSITGIRKVSSA
                     FATSSVDPGAWNLLIAKFTLGVGSDPSDTAFNYLYVNYTKFKEGDISAGGFANGDVCA
                     KASSNGGLTWGQEINLTNTNSNGCAPGDCESEHWPSLAERVDSFLYVQYIYDRDAGGI
                     PNGEGTHTNNPVRYLKYPRFLVPAIPHLSFTPDQMTDPIRWATKDGITSDSMLFSNED
                     GTADLYVKLSGPSWVIINPYNFNIAEGGPAQAVNITFNGAGFADTLLVDSLKVLSNHG
                     LTGGGEVYADTKWVKVHFVVTNEFYYPEYDTINTQAMLTTVSNVGNLGNQEDSGGMFY
                     NGHDYLYDFSPVVLVNMPGSSPPEWHASTRMYDNHDLLPESHLQVTDYPNLRATMVTK
                     TFAPTFPRLDPPWHCYWPWWTIQDKSIFFWKPARVPTKPIYYERAILKYTKFFKNPPP
                     PWWYDTWEPPNLPSTYLGFAGDWDVPSEAYDKNIGGYGDIRGPNNLIWLYADTSAFHN
                     YYAGFRFCYAERDGDTSFTPFGAHVLSNATQIGPFGGYNDDSLYKYMSTPGWSVESDS
                     AQNMNILVSGIELIDPPPTTQVTMKYALLVTDQDKADFESLATQLGRAKCGDANVDGN
                     VTVSDVVYLINYLFKGGPEPWMYFSDASGDGKISVSDAVYLINYLFKGGSPPRCDYPG
                     FAP"
     gene            complement(10955..11386)
                     /locus_tag="VGB16_03605"
     CDS             complement(10955..11386)
                     /locus_tag="VGB16_03605"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEX9916800.1"
                     /translation="MSKKYLFLVLFIPLLLWQCSTGPNPRKTVLDFLEAVHKSDSVAV
                     LFYADLDRIAEQRLSNFPPEQREKLLSVMKNDLLKSLLENGSTRIWWENCLKVVAGEK
                     VLKDQAEVEVTLIDQKTGVKHYTKMKLYLKDKMWRIYYFEE"
     gene            complement(11470..>11826)
                     /locus_tag="VGB16_03610"
     CDS             complement(11470..>11826)
                     /locus_tag="VGB16_03610"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEX9916801.1"
                     /translation="PLWFDLALRIPQGGERFVLSEVEGSRTTYHLFEPEPQSRRPEYV
                     EGQTWFLLFVGAIHELPLQCFVIPEGLIGDPYLLSFSGFPVSSTGQILLEFIPQKCGT
                     GMTQKLTNCPHSHLYR"
BASE COUNT         3322 a         2591 c         2422 g         3491 t
ORIGIN      
        1 gatcctcccc aatgggaaga gggccatcat cataagccat atccatataa gccgatggaa
       61 caaaaagatg ccgaggaact tttctggtcg catctaaaac taacttattg tgtaccccgc
      121 ggctttctat ctgctcaacc accatttttt cccttaactc gagataaata tcctctggcg
      181 atttatcttt gtccatctct tcttccttgc aggagaccag agcaaataga atagctccag
      241 tcaaaaatag cacccaggct ctcataactt ttctctttaa aaatgataat cttttgagtc
      301 tgaactgtca atatcttatt ttcttcaatt cgtcctcagt taaattatcc tctccttgat
      361 tagctatctc caagacgatt ccatctaaac agattatgat ttcctgtccc agcgagaaat
      421 atttgcccag cccgggaagg ttattcaaga gtttaaaata ggcttggctg aactttttaa
      481 ttttcaaggt gggtcgattt ccatcataac tggcatctat ccagattcca tttctgttga
      541 agaaagtttt accttccgaa gttattacct ggcttatcct gttaagtttt ccttgtgaat
      601 cgatgtaggc atctgccggt gctcgtgagc ctttcatctt agccacaccc aaagcttgat
      661 tgaccgccaa agaacccact tcctctttca gatcagccat cctttgtgca gtgtactcaa
      721 ctgactggct atgactcagg tgactatcag catcaatcag gtacgaggta tattcggtga
      781 tgatcccata ttccctagag agcctgacta tctcatctat tatttcccta ttctctccgt
      841 gcaatcttat ctggtcgatt aaatagccga ttctgcgggt ggcccaaagc ttgggaacaa
      901 aatctgcgtc agatttcttt tcgaaatctg cttcatacct aaaagtcttt ggcattccac
      961 tgactttacc ggtcagaaca acaccagcct tatgatctcc cttgaacttg cccaaaatca
     1021 ccagttgagt tcctttgaag atatccggca accggcttgg atagatatca tatacttcaa
     1081 ctcccgtaaa ttctaactgg acatcgctta agataggatt agcaactttt cgataaaagg
     1141 atgagacctt cacctcgata tcctctcccg gcttgatata atcggataac cctttgtttt
     1201 gttcagccag attatccaga aaattggtat tcacatcgta ccccaagccg aaaacgaaaa
     1261 gtctggtgct ggtcctgttt tcttggctga tattttgaag tatggtctta atatcagttt
     1321 gcccaactgt aggcaatcca tcagtcaaga aaactatcat gctcagggat gaactatttt
     1381 ttatctgatt taatccctca agcaaggcgt catttatatt tgttccgcca tcagctgagc
     1441 aaagactaat gaaatcgatt ccctctttga tattctgagc tgtggctggc actaaactgc
     1501 tcctaagact tcttatccta tcatcaaagt cgatgagatt gaatttgtct tgcggattga
     1561 gattgtttaa gcaaaagacc aaagcgtctt tggcttgcct tatcttatcg ccagacatac
     1621 ttcccgaact atctaaaata aggactatgt ttttgcctac aactttttgg gaggatatgt
     1681 ctgcctgggg agaaagcaat cccaagaaga aattaccata agtgaggata tggaacccaa
     1741 tctcgtctcg ggaaaccgtg taaaataatt tgaaatcttt atccggcttg gtccctcttt
     1801 cggtgtatgt aattcttgct tgtttgtttc caagtttttc aattttgata tcatgggttg
     1861 gggaatagat ggtctcaatc ggtagatcag aacttaggtc aatattaatc cccgctctct
     1921 ctaatggatc ttgcgagaat ttctctgtgc ttaaaagata ggtgtaactt aaaatcccct
     1981 cttccttttt aagaatctcc gaatatttta ttctgatcct caccctgcct ttggctggga
     2041 taggatagac cctcgcccta aacatcccct ggtcataata ctccaatagg gctggatcct
     2101 ttttccttct gactatctct tcataaattt ttcgagcttg ccccttatcc aaaatctcgc
     2161 ctttcagttc cctttcaccc acgaacattg aaaaatcggt aatgcttgcc cctttgggaa
     2221 ttgggaagat gtagattcct tctagatctt ggtgatgatt attcacaaac acctgatcga
     2281 ttgaggtctg gcaaagctga tttgtgattc tgacatccac ctgatgataa agcacactta
     2341 aaggcggagg aagaatcctt tgacgcgggt ctggctggat gatgataaaa ccgtctgccc
     2401 aagcagaaga gaccaaaagc ccaaccaagc tggctactat taagataagc cttttcatat
     2461 ttccctccct atggattaaa ggttgttttt ttgtttctta accttaatac aaataaggag
     2521 gaagattatt cccttaaaaa tttagttttt atgaaaggat tccagttgga tcaggatgtg
     2581 attgatagag tctttatatg tgttggttac agcggagtct ttgtaaactt ttacactatc
     2641 catggcctcc tttaatgtct cgtccttatt tctggccaga tcatataacc tcatatagcc
     2701 ctgtgccata ttcaaatacg ctttgtcttt ttcttctcct ttcggcttat cttcaatgaa
     2761 ccttctccat ttgtcgatgg actctctgac tagttcttcc tgttttacgg tgtcccctct
     2821 aacggcggcc atagtcgcct ttgatttgga actttcagct gaagtcatta caggagtgcc
     2881 ggagatagga gtttctagtg cttgaactcc cgtatgtctt tcctctttct cttttaattc
     2941 aggtgtagtg gttggtagtt tctttaaagt cgtctcgccc ttgtcaggag cgattctatc
     3001 agcaattgga gtttctttct gaacgcccct ttcctcacca ggagctacac tttctaatcc
     3061 tttgctaaca gtcatatcct ttagaggagc ttggattgtt tttgaaactt ctctttccgg
     3121 ggctactccc cctctttcgg cgatggctgg ttttctttgc tcccggggaa tgtttgtttc
     3181 aaccccaaga gtctctccgg ctggaggcga gatcttctgc acctgtgctt tttggccaag
     3241 ggtggcaata tttatctttt cataactaac aaaaagctta actaccacaa agactaagat
     3301 aaccgagaca actgcagctg cccatttgac tttggttgga gaataagaga aaatcatctc
     3361 aaggaagtaa gagaatctct gcccaaagct tactttggtt ttgtcttcga ttctttcctt
     3421 taccctatgg gcaaagcttt cccagtaagt ttcagatggt tggggaacct ttttttcttt
     3481 tacttcagct cgaataagtt tcaaatccgc taatttcttc gaacaattgg ggcatatctt
     3541 aatgtgttct cccacttctc ttttttcttt ctcgtccagt tccccatcct cataaagata
     3601 gagaagtttt tctatttggt cgcatctcat ttaatcacct ctgaggtatc cttttaggtt
     3661 ttggaccaaa cgcttccttg ccctgaataa tcgggacatc accgttcctt ttgatatctt
     3721 cagtgtctgg gcaatctgtt cgtaactttg gtcctcaagc tcccttaaga tccagaccgc
     3781 cttcaatttt gaaggcaaag agttgatttc cttgtctatt ttttgggata actccttttt
     3841 gagcagttcg gtttcaggat tatccttttc cactgcaaaa tcaattggac ttttgttttg
     3901 ctcaaatgcg ccttccgaaa cagttaagct ttccctctta atatagttca gacctaagtt
     3961 catacatatg cggtaaatcc agctataaaa actacatccg agatggaaac tcttcaaacc
     4021 ataaaaggcc ctgacaaagg tctgttgagc aatctcatct gcagcatcat gacttttcac
     4081 taatttatac gccaaaccgt atatcaccct ctcgtatttc ttgatcagct tctcaaagga
     4141 actatagttg ccacctttgg cttctcgtat taattggtcc tctaaaggtg aggattgtga
     4201 agctgacatc ctaccccttg gtaatacaat tttgatcgtt aaatattccc ccttttagat
     4261 tacacagctg ataaaccaaa agctccatcg ccagcttatt gggaaggcgg ttggatttta
     4321 tctccaggtc ggcttgatgt aaaaacaaaa gagcttgctc caattccttc caagtgaagt
     4381 tctggacttg ggatttgtaa tcccgaacaa agaagggtct tattcccaga taggtggaaa
     4441 gtggcacccc ggatttcttt tcagaaaacc ctttggtctt gaccaaacgg atgaaatgct
     4501 gagtcagcca atagattatt gtaccgggtc gttctccagc caaaaggagc ttccctaaaa
     4561 tagaaaaaga tttcttatta tctttccagc cgaccgagtt tgccaattcg aagacgttgg
     4621 tggtcttgct ttgtccaacc actgtcttta catcctccag tgttatcctc tcccgcttac
     4681 ccagaaaaga gtataacttc tcgatttcat tggctaaatt ggggagactg tttcccacac
     4741 tgttttgcag aagctcgaga gcattttggt caagtttcat cttataactt tttacccggg
     4801 ttattatcca atccgggatt tggttatcat acagagggtt aaactctaca gttgtggcta
     4861 tttgattcag ctctttgtaa aacttgttat ccattttgac cttggctgaa accaagacta
     4921 ggcaagtggt tttgggtaca gctggaagga aaccaagcaa agcctcttta cctttcgggg
     4981 aaagcttttc gagttcgtaa agtataacta cccttttggg attattcaaa ggaaaggtgg
     5041 agcaactgtt tattatctgg tcaatttcca cctggctagc atagagtaga ttaaaattga
     5101 agtcttttaa cttgtccgga accaaaagtt ttatcagctt ggagatagct tcatttttta
     5161 agaattcctc ctcaccggta aaatgatagg tcggctctat tttctttcgc tcaaaacttt
     5221 tgattaaatc cgaatagttc atttttatac aagacatgtc agaatacccc cgtaggggga
     5281 taaatgacat ttctataagt tccctctcct ttgggggaga gggttagagc ctgccctgtc
     5341 ttcgaccctg aggctcagac ccgaagggaa cgaagcgaag gggtgagggg gacagcgaag
     5401 atgattctca ccctctctcc gcctttggcg ggattttcaa cttagtcctc cccctctaag
     5461 ggggaggaga tgttttggac gcccccttag agggaaagtc actccatcca aatatcagtt
     5521 aagaaaacaa gaagctcaga tggagtataa agcttcagct ttattgagtg agttaagggc
     5581 ttacacttct catcaaatat cagctaagag cacttgtttg tcaacaataa gttcatcctc
     5641 caatggagta taaaacttta gctttattat tcttgtttac acttcaagtt aaatcgagac
     5701 tctttcaaaa ttgcttgaca tagagttgga ataggattac ttaagttcga taaaggagaa
     5761 agagttgtat caattgcaca aactgcgctg gcaggaaaag gcgattctgg ttggggttaa
     5821 gctgccacag gtcaataggt ttgcggctga agattcgtta gccgaacttt ccttattggc
     5881 aaagacagct ggggcagagg ttctggaaag cattatacag gataggaaga aaattgatgc
     5941 gggattgttc atcggaaagg gaaaagtaga acagataaag cagctctgct tagacagggg
     6001 agccaatctg gtcattttcg acgatgactt aagcccgggg cagatattca atctggagaa
     6061 aattctggat ttgaaggtaa ttgaccgaag ctgcttggtt ctggatatat ttgccaaaag
     6121 ggcaaaaacc gcagaagcca agattcaagt tgaactggct caactgcaat atcttttgcc
     6181 acggttgacc cgtggctggc tacatctgtc ccgacagtgg ggaggaattg gaaccaaagg
     6241 tccgggcgag acccaattgg aggttgaccg gagagtaata aagaagaaga taggggattt
     6301 gaaaggaaag ctggtcaaga ttgacaaaga aagacaaacc cagagaaaga aaagagagga
     6361 ttttttcaag gtggccttgg tgggatatac taatgtagga aaatcaactc tctttaacca
     6421 gcttaccaaa gcgaccgttc cagttgagga aaaactattc tccactctgg attcctttac
     6481 tcgcatttta aggttctcca gaaatcataa tattctcttg actgatactg tggggtttat
     6541 acgcaagctc cctcatcatc tggtggctag ctttcgttca accttagacg aggttaagtt
     6601 agccgacctg ttattgcata tagtggatat aagccatccc aacttccctc agcagatgga
     6661 gaaggtcagc cagatcttaa aggagctcga ttgctcaagc aagcccactc ttttagtttt
     6721 caacaaaatc gataagctca aaggggacct gcctttgggt ctttgccctt cttatgaaaa
     6781 cgagccgatt tttatatccg cggagaaagg aattgggctg gcaaatttaa tcgccagcct
     6841 cgagttgttc atggatgaac agtcttgtga agcggaggtt ttcttaaaaa aagaggatct
     6901 gaacctcttg ccctcttttt acaaattggg aatagtaacc caaaccgaga tgaaggacga
     6961 aggtctaaat ctgaaaataa gagccaaaaa atggaatctg gagaaactca aaggcttggc
     7021 aaatggtgaa gatttcgctt gacagaaaag ctcgggttta gttaatatta tgcaaaagcc
     7081 ctcttcccat aacacaaacc tggtcctatg ccaaaagata aaaagatatt gattgtggat
     7141 gataatccca atatgtcgaa tttgctttca gacatactgg agatatttga atgccaaagc
     7201 gtttgcgctc cggatggaga ggaggccttg aaatgtctga aaagccagaa gttcgatatg
     7261 gtgataactg acctcaggat gcccaagatg cacggaattg acctgttgaa aacagtcaag
     7321 gagaattatc ctggtttacc ggtggtggtg ataaccggtt tttccggaga taccaccatg
     7381 gacgaagcca tagatgccca tgcggatgga tttttggcaa agccgttcag ggtgaatgat
     7441 attcagaaac ttttggaaag acttttaggg tagagaaatt agtttcttat agaagtaaaa
     7501 aagcaaaggg caggaacaaa tcctgccctt tgtaatttca aactcatctt tgacatagat
     7561 cacggagcaa atccaggata atcacatctg ggcggagagc ctcctttaaa cagataattg
     7621 attaaataca ctgcatctga aactgaaatt ttcccgtctc cgctagcatc agagaaatac
     7681 atccagggct caggccctcc tttgaacagg taattaatca gatatactac atctgatact
     7741 gtgacattgc cgtccacatt agcatctcca catttggctc ggccgagctg agtagccaag
     7801 gactcaaaat cggctttgtc ctgatcagtt actaacagag catacttcat tgtaacttgg
     7861 gtggtgggag gtgggtcgat tagctcaata cccgagacca atatattcat attctgagca
     7921 gaatccgatt caactgacca gccgggagtg gacatatact tatacaagga gtcatcattg
     7981 tacccgccga aaggacctat ttgagttgca ttgctcagca cgtgggcgcc aaaaggtgta
     8041 aaactggtgt ctccatccct ttccgcatag caaaagcgaa agcccgcata gtaattatga
     8101 aaagcagaag tatccgcgta tagccagatg agattattgg gaccccgaat atcgccatag
     8161 ccacctatgt ttttatcata agcttcagat ggaacatccc aatcgcctgc aaagcctaaa
     8221 taggtggaag gtaaatttgg tggctcccag gtatcatacc accaaggcgg aggaggattc
     8281 ttgaagaact tagtgtattt tagaatcgct ctctcgtaat aaataggctt ggtaggaacc
     8341 cttgctggtt tccagaagaa tatgcttttg tcctgaatag tccaccaagg ccaatagcaa
     8401 tgccaagggg gatccagtct ggggaaagtt ggggcaaaag ttttagtaac catagtagcg
     8461 cgcagatttg gataatcagt tacctgaaga tgtgattcgg gtaataagtc atgattatca
     8521 tacatccgag tagaagcatg ccattcaggt ggagaggagc caggcatatt gaccaacacc
     8581 acgggtgaga aatcatagag gtaatcatgc ccattataga acatcccccc gctatcctct
     8641 tggttaccca agtttccaac gttagagacg gtggtgagca tcgcttgagt gtttattgta
     8701 tcgtattcgg gatagtaaaa ttcattggta accacaaagt gaaccttcac ccatttagta
     8761 tccgcatata cctctccccc acccgttaat ccatgattgg acaaaacctt taaactgtca
     8821 accagtaaag tatctgcaaa accagctcca ttgaaggtaa tattgacagc ttgagcaggt
     8881 ccgccctccg caatgttaaa attatacggg tttataataa cccaggatgg tccgctcaat
     8941 ttaacgtaca aatcagctgt gccgtcttca ttactaaaga gcattgaatc agaagtaatg
     9001 ccgtccttag tggcccatct gattggatct gtcatttggt caggggtaaa agagagatga
     9061 ggaatggctg gaactaagaa acgaggatac ttcaggtaac ggaccgggtt gttggtgtgg
     9121 gttccctcgc cattcggtat cccgcctgca tctctgtcat aaatatactg tacatatagg
     9181 aaactatcca ccctttctgc cagggaaggc cagtgctcgc tttcgcagtc gcccggagca
     9241 cagccatttg aattagtatt agtaaggttt atttcctgtc cccaggttaa cccaccattg
     9301 gaagaagcct tagcacacac atccccatta gcaaatccgc ctgctgagat gtcaccctct
     9361 ttgaatttgg tgtagttaac atagagataa ttaaaagctg tgtctgatgg atcagagcct
     9421 actcctaaag tgaacttggc aataagcagg ttccaagctc cagggtctac tgaagaggtg
     9481 gcaaaagcag aactgacttt gcgaattccg gttatagaac tccagtgcca taaagtaaca
     9541 tcattggaat tgttctcggt aaaggtggtc cacattatgt gcaattcatc attatagtcg
     9601 taaactgcag caatatcaca ataagctcta tctctaaagc cagacgcacc atagctggta
     9661 atctggacag gagttcccat atttcctgct gccatccagt cattaccatt attagtcgat
     9721 tctaaataca tcacctcatt cttggtcgca ctgttacttg tatactgcaa ccaggcaata
     9781 caaaccttct gagaaactgg tgaggtggct atcactccac ttcctaattt agcgatagca
     9841 tacataaaag gaactttgtt tggcacaagt ttggtgtctt ttggaataag aagaggagaa
     9901 tcccaaccag gagactgaca aatcaaagta tctctatttg taggcatctc aaagcaacgc
     9961 acataaccta attccttatt ggtaacacag ccagtagaag atctctcatg agttatatga
    10021 aagtaggtgg tgtcgccatt cttcaatttg gagcaaccaa tagctggcca ctcacttttg
    10081 tctgaagtgt gtgccacaga atcagggata tcaaaatggt tgaactgccc tgagccagga
    10141 atactatctt caatagaaac agtagatccc cagtcatcac catatggtcg gtgataacac
    10201 aacacttctc tgttatccgg caggatatcc agaccaccat agccacccct aactaaatcc
    10261 ggagtgatat gtacaccacc gggattaagc caggttccgt tactgtaacg tgcattgtaa
    10321 tctatgtatc ggattccata ataatagtca ggactaatgc agtccatcca ggtaaaatgc
    10381 agagtcccgg acaagtcgct ggctatctgc ctgtgctggg gagcattaaa ctggtaatcg
    10441 taataagtgt aacccaccgt gtcatccgag aaaaggtatt taatcttaga ctctggaatt
    10501 ctaggatatt ttataggcgg aatctcagca gtatagcaaa tcgtatccag actctccaat
    10561 ttctgtatca ttttataatc tgctggctta tttgccccaa aaacgcttaa acttaatacc
    10621 agcaataaac ttatagtaaa gattataatt cctttaatca tactatctcc tttctttctg
    10681 atgctttggc aatagcatat gtcaaagcac gagggcgttg gcatatcctt ctatcccgat
    10741 gcatgctgaa agaatcaaaa aatcggattg aatagcagaa gtaagaagag aaaaagtgat
    10801 ttcaagcctt tttaactttt cacccaccct tcaaatgaac gtaggttttt taacttttta
    10861 ttaaataaat actactacta taacacgtta tactataaaa aaacgtttgt caagcgaatc
    10921 caatcgagga gttcactctt acagatacca aactctactc ctcaaaataa taaatcctcc
    10981 acattttgtc cttaagataa agcttcatct tggtataatg tttaaccccg gttttctggt
    11041 caatcagagt aacttcgact tctgcctggt ctttaagtac tttctctcca gccacgacct
    11101 tgagacaatt ctcccaccat atacgcgtgg agccattctc caagagactc ttcaataaat
    11161 cattcttcat tacggaaagg agtttttctc tttgttcagg tgggaaattg gagagccttt
    11221 gttctgctat tctatctaaa tccgcgtaga ataaaacagc cacgctatca gatttgtgaa
    11281 ccgcctcgag aaaatcaagc accgtttttc gtggatttgg tccagttgaa cattgccata
    11341 agagtaaggg gataaataga actagaaaca gatatttttt ggacatattt gactccatgg
    11401 attcgttttg ctttaaaaaa tcacaaacca aagggaatgt caactgtttt ttaagtcagc
    11461 gggaacttcc tatctgtaaa ggtgggaatg ggggcagttt gtgagttttt gtgtcattcc
    11521 tgtcccgcat ttctgcggga taaactccag cagaatctgt cccgtactgg atacgggaaa
    11581 tccagaaaag gaaagcaagt atggatcccc gattaaaccc tcggggatga caaaacattg
    11641 taggggcaat tcatgaattg cccctacaaa caacaagaac cacgtctgtc cttcgacata
    11701 ctcaggcctt cggctctgag gctcaggctc gaagagatgg taagtggttc gacttccttc
    11761 gacttcgctc aggacaaatc gctcaccacc ctgaggaatt cgaagggcaa ggtcgaacca
    11821 taaagg
//
LOCUS       DASWLN010000106         6655 bp    DNA     linear   ENV 20-FEB-2024
DEFINITION  MAG TPA_asm: Candidatus Zixiibacteriota bacterium isolate
            SMAG_U2949 k141_731555, whole genome shotgun sequence.
ACCESSION   DASWLN010000106 DASWLN010000000
VERSION     DASWLN010000106.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37960594
            Sequence Read Archive: SRR10376091
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Candidatus Zixiibacteriota bacterium (soil metagenome)
  ORGANISM  Candidatus Zixiibacteriota bacterium
            Bacteria.
REFERENCE   1  (bases 1 to 6655)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 6655)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (26-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/12/2024 08:28:26
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 1,440
            CDSs (total)                      :: 1,416
            Genes (coding)                    :: 1,412
            CDSs (with protein)               :: 1,412
            Genes (RNA)                       :: 24
            rRNAs                             :: 1 (16S)
            partial rRNAs                     :: 1 (16S)
            tRNAs                             :: 21
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 4
            CDSs (without protein)            :: 4
            Pseudo Genes (ambiguous residues) :: 0 of 4
            Pseudo Genes (frameshifted)       :: 0 of 4
            Pseudo Genes (incomplete)         :: 3 of 4
            Pseudo Genes (internal stop)      :: 1 of 4
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..6655
                     /organism="Candidatus Zixiibacteriota bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_731555"
                     /isolate="SMAG_U2949"
                     /isolation_source="soil"
                     /db_xref="taxon:2053527"
                     /environmental_sample
                     /country="USA: Oregon"
                     /lat_lon="42.99 N 123.63 W"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            <1..218
                     /locus_tag="VGB16_03615"
     CDS             <1..218
                     /locus_tag="VGB16_03615"
                     /EC_number="2.7.2.7"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_009172690.1"
                     /note="catalyzes the phosphorylation of 2-butanoate to
                     butanoyl phosphate; Derived by automated computational
                     analysis using gene prediction method: Protein Homology."
                     /codon_start=3
                     /transl_table=11
                     /product="butyrate kinase"
                     /protein_id="HEX9916802.1"
                     /translation="EIGAMATTLLGKVDGIVITGGIANSKKLVNSIEERVKFIAPVMV
                     FPGEDEMEALTLGVLRVLNKEEEAKEY"
     gene            complement(219..488)
                     /locus_tag="VGB16_03620"
     CDS             complement(219..488)
                     /locus_tag="VGB16_03620"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEX9916803.1"
                     /translation="MKITEGLSNLIPSAVGILLGLSSFLFSFLATLSILSLVTSVSAI
                     GLCGFSLEKSGRAKLLPIIGLVLGALGFIWSILVHSNLPNNGYSM"
     gene            558..1463
                     /locus_tag="VGB16_03625"
     CDS             558..1463
                     /locus_tag="VGB16_03625"
                     /EC_number="2.3.1.19"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_018206683.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="phosphate butyryltransferase"
                     /protein_id="HEX9916804.1"
                     /translation="MKSLNDILNLAKRIGPKRVAVALAEDEGVLVALKRAKKENAIEG
                     ILVGTKEKIISLADKNEIDLKNFQVIDQPDEFEASLLCCELINQGRADVIMKGLVSTG
                     TFMKAILYKEKGLEKGKLLSHVAVFEIPNYPKLLLITDAAINIAPNLAQKAQIIQNAI
                     EVAHSLGIGNPLVACVAAVEKVNPKMPATVDAEALATMSKKGEIKGGIVDGPFGLDNA
                     VSEESARIKGVRSPVAGKADIILAPDIECGNVIYKTLIEFGQAKCAAIAIGAKAPVIL
                     TSRADSDETKLMSIALGVVNTVKSK"
     gene            1474..2397
                     /locus_tag="VGB16_03630"
     CDS             1474..2397
                     /locus_tag="VGB16_03630"
                     /EC_number="2.6.1.42"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_004029520.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004084 - branched-chain-amino-acid
                     transaminase activity [Evidence IEA];
                     GO_process: GO:0009081 - branched-chain amino acid
                     metabolic process [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="branched-chain amino acid transaminase"
                     /protein_id="HEX9916805.1"
                     /translation="MAFEKADKIWMNGELVGWDDAKIHVLSHVVHYGSSVFEGMRCYK
                     TKKGPAIFRLSTHTDRLFNSAKIYRMDIPYTKEEINQATIDLIKANKLDECYIRPIVF
                     RGYHHLGVDPTGCPIEVVIATWKWGKYLGKDALEKGVPVCVSSWNRMAPNTFPAMAKA
                     GANYMNSQLIKLEAIGHGYVEGIALDVFGQVSEGSGENIFIVKDGVLITPPFGSSILP
                     GITRNTVITLAHQIGIKVIEETIPREALYIADEVFFTGSAAEISPISSIDKIIIGTGA
                     RGPITKKLQEKFFKLLSAEEEDMYNWLTYVR"
     gene            2417..3313
                     /locus_tag="VGB16_03635"
     CDS             2417..3313
                     /locus_tag="VGB16_03635"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_008907395.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016746 - acyltransferase activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="bifunctional enoyl-CoA hydratase/phosphate
                     acetyltransferase"
                     /protein_id="HEX9916806.1"
                     /translation="MITNFDELIKLVKSKPTRRLAIASAEGEEIILAVKQAVEEHIVF
                     PILIGDREKIETICKKVDFDTKGCQIINEKDMEQAASKAVSLVKDKTAEMLMKGKVGT
                     STLLKAVLDKEKGLRSGRLLSHVSVIQIKRYPKLMLVTDGGMNIKPDVNKKVEIIMNA
                     VEVAKKLGMDIPKVACLAAIETVSPDMPETTDAQAITEMAKRGDIKGVIVDGPMALDV
                     ALDSKSAEIKGIKSEVAGDTDIFLVPDIASGNIFVKGLIYLADAKVGGIVVGGGAPIV
                     LLSRSDSAETKLYSIALGAAIC"
     gene            3692..4135
                     /gene="rpiB"
                     /locus_tag="VGB16_03640"
     CDS             3692..4135
                     /gene="rpiB"
                     /locus_tag="VGB16_03640"
                     /EC_number="5.3.1.6"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_017890330.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004751 - ribose-5-phosphate isomerase
                     activity [Evidence IEA];
                     GO_process: GO:0009052 - pentose-phosphate shunt,
                     non-oxidative branch [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="ribose 5-phosphate isomerase B"
                     /protein_id="HEX9916807.1"
                     /translation="MRIALGADHAGYKIKEMVKKHLLRLGHQVEDFGTTSQESVDYPD
                     YAFKVAESVAKGDNERGILFCWTGNGMCICANKVKGVRAAVCLNEDMASLSREHNQAN
                     ILCLGSKFVPENKVIPIVNAWLRSEFVGDRHERRVNKITKYEEKK"
     gene            4132..5385
                     /gene="glyA"
                     /locus_tag="VGB16_03645"
     CDS             4132..5385
                     /gene="glyA"
                     /locus_tag="VGB16_03645"
                     /EC_number="2.1.2.1"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_010942252.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004372 - glycine hydroxymethyltransferase
                     activity [Evidence IEA];
                     GO_function: GO:0008270 - zinc ion binding [Evidence IEA];
                     GO_function: GO:0030170 - pyridoxal phosphate binding
                     [Evidence IEA];
                     GO_function: GO:0070905 - serine binding [Evidence IEA];
                     GO_process: GO:0019264 - glycine biosynthetic process from
                     serine [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="serine hydroxymethyltransferase"
                     /protein_id="HEX9916808.1"
                     /translation="MSYLKKVDPEIYEAILNETKREANNLELIASENFVSEAVLEAQG
                     GVMTNKYAEGYPGKRYYGGCEFVDVVEELARKRAKMLFNCEHVNVQSHSGSQANMAVY
                     FTVLNPGDTILGLSLACGGHLTHGHPVNFSGRFFKVASYGVSKETEQIDYDELLKLAR
                     EHKPKMIVTGASAYPRVLDFAKFREIADEVRAYLMVDIAHIAGLIVAGLHPSPVPYAD
                     FVTTTTHKTLRGPRGGMIMCKEKYAKDLDRTVMPGIQGGPLMHVIAAKAVAFKEALEP
                     SFKEYQKQIVKNAKTMAEEFKKLGYHVVSGGTDTHLMLLSFVGRNITGKEVELALDKA
                     GITANKNAVPFDPQKPFVTSGIRIGTPAVTTRGMKEDQMKEIANLIHQVISNLNREEV
                     YEEVKRKVTELCKKFPLYQERITKE"
     gene            5393..5803
                     /locus_tag="VGB16_03650"
     CDS             5393..5803
                     /locus_tag="VGB16_03650"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEX9916809.1"
                     /translation="MDYVDRKFPALAVLLGGLIPGIWGVLHLSFTESVIMEYSSLSEE
                     LLETLRLEWVIEGVFLLFISILIIALYPHFRRKSKVARKAGFYTGAALVVVGIWHASV
                     PALNGLFYHLYTPLTILSAFLIWLPLIFHRGEWN"
     gene            6016..6216
                     /locus_tag="VGB16_03655"
     CDS             6016..6216
                     /locus_tag="VGB16_03655"
                     /inference="COORDINATES: protein motif:HMM:NF022229.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="winged helix-turn-helix domain-containing
                     protein"
                     /protein_id="HEX9916810.1"
                     /translation="MHDKVGSTAGEMWKYLHERGESTVSKMVKELREKDSVIYMGLGW
                     LARENKVAIRQDKTAIRVKLTE"
BASE COUNT         2075 a         1211 c         1656 g         1713 t
ORIGIN      
        1 aagagatagg tgctatggcc accacccttc tgggaaaagt tgacggcata gtgataactg
       61 gcggaattgc caattcaaag aagttagtca attcaataga ggagagagtg aaattcatcg
      121 cgccggtcat ggtttttccg ggagaagatg agatggaagc gttgacttta ggggttttaa
      181 gggttctgaa taaagaggag gaagcaaaag aatattaatt acatcgaata accattgttc
      241 ggaagattgg aatgaaccag aatagaccag ataaatccca aagcccccag gacgagtccg
      301 attataggca gaagcttggc acgacccgat ttttctaaac tgaaaccaca aagtccgatg
      361 gcgctgacac tggttaccaa acttagaata cttaaagtgg ctagaaaaga aaaaaggaaa
      421 gagcttaaac ctaaaagtat cccaactgcg ctggggatta aattagataa accttctgtg
      481 attttcatag tataggagac aatcaagaca gttcttaatc attaaaatac tgacataaaa
      541 caagactgtc aagcaaaatg aaatcgctta atgatattct gaatttagct aagaggattg
      601 gtcccaaaag agtggcagta gcattagctg aagacgaggg tgttctagtg gccttaaagc
      661 gggctaaaaa ggaaaatgcg atagaaggga tattagttgg caccaaagag aaaattatct
      721 ccttagcaga taagaacgaa atcgacctga agaactttca ggtcatagac cagccagatg
      781 agtttgaggc ctcactttta tgctgtgaac ttataaatca gggcagggcg gatgtgataa
      841 tgaagggttt ggttagtacc ggaacgttta tgaaggcgat attgtataaa gagaaagggt
      901 tggagaaggg aaaactcttg agccatgtgg cggtatttga gataccaaat tatccaaagt
      961 tgttgttgat tactgatgca gccataaaca tagctccaaa tctggcacag aaagcccaaa
     1021 tcatacaaaa tgccattgag gtagcgcatt ccttgggaat aggtaacccg ttggtggctt
     1081 gcgtggcagc agtggagaag gtcaatccaa aaatgccagc gacagttgat gccgaagctt
     1141 tggctactat gtcaaaaaag ggggagataa agggtgggat agtcgatggt ccgtttggct
     1201 tagataatgc tgtctcagag gaatcagcca gaattaaggg agtgagaagc ccggttgccg
     1261 gaaaagcgga tataatcttg gctccggata tcgaatgcgg aaatgtgatc tacaaaactt
     1321 taattgaatt cggccaagct aagtgcgccg caattgcaat aggagcaaag gcaccggtga
     1381 tcttgacctc gcgcgcagat tcagatgaaa ccaaacttat gtccatagca ttaggtgtgg
     1441 tcaatactgt gaagtcaaaa taaaggagga ggaatggctt ttgagaaagc agacaagata
     1501 tggatgaacg gcgaactggt aggttgggat gatgctaaga ttcatgtgct ttcccatgtg
     1561 gtgcattatg gttcgagcgt gtttgaggga atgagatgtt acaaaaccaa aaagggacca
     1621 gccatattca ggctttcaac tcatactgac aggcttttca attcagccaa gatctatcgt
     1681 atggatattc cctataccaa agaggagata aaccaggcca ccatagattt gataaaggcg
     1741 aacaagttgg atgagtgcta catccgtccc atagtttttc gagggtatca ccatttggga
     1801 gttgacccca ctggatgtcc aattgaggta gtcatcgcaa cctggaaatg gggaaagtat
     1861 ttaggcaagg atgctctgga gaaaggggtt ccggtttgcg tttcttcctg gaatagaatg
     1921 gcacccaata cctttccggc tatggctaag gccggggcaa attatatgaa ctctcaactc
     1981 ataaagctgg aagctattgg gcatggatat gttgagggaa tcgccctgga tgtttttggt
     2041 caagtgagtg agggaagcgg ggaaaatatt ttcatagtga aagatggggt gttgattact
     2101 cctcccttcg ggtcttccat tttgcccgga ataaccagaa acacagttat caccttagcc
     2161 caccagatag gtattaaggt aatcgaggaa actattcctc gcgaagctct atacattgcg
     2221 gatgaggttt tctttacggg aagtgcggcc gagatttctc ccataagcag cattgataag
     2281 ataatcattg gcacgggggc tagaggccca atcaccaaga aactgcagga gaagttcttt
     2341 aagcttctgt cggctgaaga agaagatatg tataattggc tgacttacgt tcgttaaggt
     2401 tcaaggagag agataagtga ttactaattt tgatgaattg attaagctgg tgaaaagcaa
     2461 acccacgcga aggcttgcta tcgcctcagc tgaaggagag gagataattc ttgcggtcaa
     2521 gcaagcagtt gaggaacaca ttgttttccc catactaatc ggcgaccgag aaaagataga
     2581 aaccatctgc aaaaaagttg atttcgatac aaagggctgc cagataatca atgaaaagga
     2641 tatggaacag gcagcatcta aggcagtcag tttagtgaaa gataagactg ccgagatgct
     2701 gatgaagggg aaagtgggaa cctcaacact tttgaaagct gtcttggata aggaaaaggg
     2761 gttgcgaagc ggcaggcttt tgagtcacgt ctcggtcatc caaataaaaa gataccccaa
     2821 gttgatgctg gttacagatg gcggaatgaa catcaagccg gacgtcaata agaaagttga
     2881 gattataatg aatgcggttg aggttgccaa gaagttagga atggatattc ccaaggtggc
     2941 ttgtttagct gcaattgaga ccgttagtcc ggatatgccg gaaacaacag atgcccaagc
     3001 cataactgaa atggcaaaac gaggggatat caaaggggtc atagttgatg gacccatggc
     3061 tttggatgtt gcccttgatt ccaaatccgc tgaaataaag ggcataaagt cagaagtagc
     3121 tggcgatact gatatatttt tggttcccga tattgccagt ggaaatatct ttgtgaaggg
     3181 cttaatctac ttagcagatg ccaaagtagg cggaatagtt gttggtggag gggcgcccat
     3241 agtattgctt tcacgttcag acagtgcgga gacaaagctg tactccattg ccctgggagc
     3301 agcgatttgc tgaaggtcat ttgagaattt gaacttgaat aatgcagatt gccacgtccc
     3361 gccccgattt tatcgggacg ggactcgcaa tgacactata tttgtttgtc attcctgtgc
     3421 cgccttaagc ggggtaaatt tcaacaggaa cctatttggg tttatcggaa ttatggattc
     3481 ccgctttcac gggaatgaca gaaacataga tttttacgcc taagcaaaat gatagcccaa
     3541 ttctgtcatt gcgagtccgt aggacgaagc aacctggttt ctatgatcta taacgacatg
     3601 aattctacaa agcgattaca gaaacggcat gaaaatacgc attatatttt gataaaatgt
     3661 tttaacaaaa taaagcaata ggggcaaatt tatgcgaatt gcattaggag ctgaccacgc
     3721 tgggtataaa attaaggaga tggtaaaaaa gcatctctta agattaggac atcaagttga
     3781 agatttcgga actacctctc aggaatcagt agattatcct gattatgcct ttaaggtggc
     3841 tgaatcagta gccaagggcg ataacgaaag aggaatactc ttttgctgga cgggaaacgg
     3901 aatgtgtatt tgcgctaaca aagttaaagg tgtgcgcgca gcagtctgtt taaatgaaga
     3961 tatggcttct ctttccagag aacataacca ggcaaatatc ttgtgcttag gctcaaagtt
     4021 tgttcccgaa aataaggtta ttcccattgt caacgcttgg cttaggagtg aattcgttgg
     4081 ggataggcac gagcgaaggg tgaataagat cacaaaatat gaggagaaga aatgagttat
     4141 ttaaagaaag ttgaccctga aatctacgaa gcgattttga atgagaccaa aagggaagca
     4201 aacaatttgg agcttatcgc ttcggagaat tttgtcagcg aggcggtatt ggaagcccaa
     4261 ggcggagtga tgaccaataa gtacgctgaa ggttatcctg gtaaaagata ctatggggga
     4321 tgcgagttcg tggacgtagt agaggagtta gcccgaaaga gagcaaaaat gctttttaac
     4381 tgtgagcacg tcaatgtcca gtcccattcc ggctcacagg cgaatatggc ggtctatttc
     4441 acggtcttaa atccgggcga caccatttta ggtttaagtc tggcttgtgg cggtcatttg
     4501 acccatggtc atccagtgaa tttctccgga agatttttta aagtggcttc ttacggagta
     4561 agcaaggaaa ccgaacaaat cgattacgat gagttattaa agcttgccag agaacacaaa
     4621 cccaagatga ttgtaaccgg agcttcggct tatcctcgtg ttttggattt cgcaaaattc
     4681 cgggaaatag cagatgaggt gagagcatat cttatggttg acatagccca cattgccggt
     4741 ttgattgtgg cgggacttca tccctctcct gttccttatg ccgacttcgt caccaccacc
     4801 acacataaga ctctaagagg tccgagaggc ggaatgataa tgtgcaagga aaaatatgcc
     4861 aaggacttgg atagaacagt tatgcccgga attcagggtg ggcccttgat gcatgttatc
     4921 gccgcaaagg cagtagcatt taaagaagct ctggagccat ccttcaagga gtaccagaag
     4981 cagatagtga aaaacgccaa gaccatggca gaggagttta agaaattggg atatcacgtc
     5041 gtgtccggtg gaacggatac tcacctgatg cttctgagtt ttgtgggaag gaatataacc
     5101 ggcaaggaag ttgagcttgc tctggacaaa gccggaataa cggcaaataa gaatgcagtt
     5161 cccttcgacc cacaaaaacc ttttgtcact tcaggcataa gaattggaac tccagcagtt
     5221 accaccagag gaatgaaaga ggatcagatg aaagaaatag caaatctgat tcatcaggtg
     5281 atttcaaatc taaataggga agaggtgtat gaggaagtga aaagaaaggt tactgagctt
     5341 tgcaagaaat ttccattata ccaagaaagg ataacaaagg agtaagttca aagtggatta
     5401 tgttgacaga aaattcccag ctctggctgt tcttctgggt ggactcattc ccggaatctg
     5461 gggtgttctg cacctgtcgt tcactgaatc tgtgataatg gaatatagct ctctttccga
     5521 ggagctactt gagactcttc gcctggagtg ggtgattgag ggagtctttc ttttattcat
     5581 ttctatttta ataattgccc tctatcctca cttccgacga aaaagcaaag tggcgcgtaa
     5641 agccggattt tacactgggg cagctttggt agttgtgggc atttggcatg ccagtgtgcc
     5701 agctttgaat ggtttgtttt accatcttta cacaccactt accatactct ctgcgttttt
     5761 gatctggctg ccattgattt ttcatagagg ggaatggaat taggaaagct cttcgttgaa
     5821 taggtttcaa caggtgctaa aggcggatag ggaaattcta aagattgtaa tgtagtaaga
     5881 aaaaaaagtt gccaagtccg attaaaaagg ttaaattaat accgatatag gtaatacata
     5941 acagggatgt ttcagagaat gacagtatgc tactgtcctt tcttctgaat ctaaccttca
     6001 tagcggaggc gtttcgtgca cgataaggtc ggctctacag ctggggaaat gtggaagtac
     6061 ctacatgagc gtggggaatc cacagtcagc aagatggtaa aagagcttcg cgagaaggac
     6121 agcgtcattt atatggggct tggctggctc gccagggaga acaaggttgc catcaggcag
     6181 gataagacag ccataagagt caaactaaca gaatagcaag agattgctaa tcaaaagatt
     6241 atattaaagc tttaaagtaa aagtttaagg caaatgtggg ggtttgtttt attttttccc
     6301 acttgaggtt gctgtaatca attgcatcat ttatgaatag gtttgcacaa aaactgctaa
     6361 aggaatctgg gtccaattca gaccctatca aatccacaga aactccctgt aatttggctt
     6421 tcaagcaact atttaattat caatacatta gataacttca tcctcacata aaaacttgag
     6481 aaattacccc ttcttattct tactatacca cggcatagaa tttgcatatc aagaatatga
     6541 tttactctat ccccggtaat ctatatggat gagaaaagaa acaagaatta taacaaattc
     6601 ctcctttttc gcagcttagc gataaagatc aactctaacc tccctctttc ggaga
//
LOCUS       DASWLN010000107         2773 bp    DNA     linear   ENV 20-FEB-2024
DEFINITION  MAG TPA_asm: Candidatus Zixiibacteriota bacterium isolate
            SMAG_U2949 k141_606204, whole genome shotgun sequence.
ACCESSION   DASWLN010000107 DASWLN010000000
VERSION     DASWLN010000107.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37960594
            Sequence Read Archive: SRR10376091
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Candidatus Zixiibacteriota bacterium (soil metagenome)
  ORGANISM  Candidatus Zixiibacteriota bacterium
            Bacteria.
REFERENCE   1  (bases 1 to 2773)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 2773)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (26-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/12/2024 08:28:26
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 1,440
            CDSs (total)                      :: 1,416
            Genes (coding)                    :: 1,412
            CDSs (with protein)               :: 1,412
            Genes (RNA)                       :: 24
            rRNAs                             :: 1 (16S)
            partial rRNAs                     :: 1 (16S)
            tRNAs                             :: 21
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 4
            CDSs (without protein)            :: 4
            Pseudo Genes (ambiguous residues) :: 0 of 4
            Pseudo Genes (frameshifted)       :: 0 of 4
            Pseudo Genes (incomplete)         :: 3 of 4
            Pseudo Genes (internal stop)      :: 1 of 4
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2773
                     /organism="Candidatus Zixiibacteriota bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_606204"
                     /isolate="SMAG_U2949"
                     /isolation_source="soil"
                     /db_xref="taxon:2053527"
                     /environmental_sample
                     /country="USA: Oregon"
                     /lat_lon="42.99 N 123.63 W"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            <1..667
                     /locus_tag="VGB16_03660"
     CDS             <1..667
                     /locus_tag="VGB16_03660"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003338177.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016627 - oxidoreductase activity, acting
                     on the CH-CH group of donors [Evidence IEA]"
                     /codon_start=2
                     /transl_table=11
                     /product="acyl-CoA dehydrogenase family protein"
                     /protein_id="HEX9916811.1"
                     /translation="NSSYADTFIITAVTDKSKGVKGISSFILEKGMKGFSVGKKENKL
                     GLRGSNTAELHFENLRIPKEQLLGKEGEGFKQFMITLDGGRISIGAMALGIAQGALDM
                     SIKWAKEREQFGKKIGEFQAIQWKLADMATRIEAARHLIYHSAWMKDRGLRFTKESAM
                     AKLYASEVGRFATYQAIQIYGGYGYLQKYPVERYFRDVKLCEIGEGTSEIQRLVIARE
                     LLK"
     gene            692..1468
                     /locus_tag="VGB16_03665"
     CDS             692..1468
                     /locus_tag="VGB16_03665"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_009611266.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003824 - catalytic activity [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="enoyl-CoA hydratase-related protein"
                     /protein_id="HEX9916812.1"
                     /translation="MDLKYTAMEMVEDSIAIVKINRPDALNTLNRDVLQELEEVFRKL
                     GDDDKVKVVIITGEGKAFVAGADIAQMKDMSGMQAREFSKLGQQVFGLIESLEKPVIA
                     AVNGFALGGGSELALACDIRIASDKAKLGQPEVNLGIIPGFAGTQRLSRLIGPSNAKM
                     LIYTGDIIDAQTALHYGLVNKVVPGEELMKEALELARKIISKGPNAIKFAKGVINKGI
                     QADLLTGSSYEMEAFSLCFSTGEAKEGMSAFLEKRKPNWK"
     gene            2010..>2773
                     /locus_tag="VGB16_03670"
     CDS             2010..>2773
                     /locus_tag="VGB16_03670"
                     /inference="COORDINATES: protein motif:HMM:NF014827.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016491 - oxidoreductase activity
                     [Evidence IEA];
                     GO_process: GO:0006520 - amino acid metabolic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="Glu/Leu/Phe/Val dehydrogenase dimerization
                     domain-containing protein"
                     /protein_id="HEX9916813.1"
                     /translation="MEIFKELAIDKHEQVVFCSDKVSGLRSIIAIHDTTLGPAIGGVR
                     MYPYKSEDDALKDVLLLSKAMTYKAAAAGVNLGGGQGVIMGNPDKDKTEILLRTFGRF
                     VNGLNGRYITAEDLGTTVEDMEIVHYETKYVAGLSRAVGGGGDPGPVSAIGVYYGIIA
                     CVEKVFKDKDLSKRSIVVQGLGNVGYSLAQKLADEGAKLFVADVIESKVERATKEFKA
                     EVVNPDEVFEIEADIFSPCALGGVINKTNIDKMKAK"
BASE COUNT          832 a          481 c          747 g          713 t
ORIGIN      
        1 taattccagc tacgcagata ctttcatcat cactgcggtt acggataaga gtaagggagt
       61 aaagggcata agctccttta tattggaaaa aggtatgaaa ggtttttctg tcggcaagaa
      121 ggaaaacaaa ctcgggttgc gtggctcgaa tacagcggag cttcattttg agaatttaag
      181 aattcccaaa gagcagcttt tgggcaaaga gggagagggg ttcaagcaat ttatgataac
      241 tctggatggt ggcagaataa gcattggcgc catggctctg ggaatcgctc aaggagcttt
      301 ggatatgagc atcaaatggg caaaagaaag ggagcagttt ggaaaaaaga ttggtgaatt
      361 tcaggcaatt cagtggaaac tggcagatat ggcaacccga attgaagctg cccgacatct
      421 gatttatcat tccgcctgga tgaaggacag aggcttaaga tttaccaagg aatctgctat
      481 ggctaagctt tacgcctcag aggtaggcag attcgctact tatcaggcta ttcagattta
      541 tggcggatat gggtatctcc aaaaatatcc ggtagaaagg tatttcagag atgtgaagtt
      601 atgcgaaata ggtgaaggga cttcggagat tcaaaggttg gttattgcca gagagctttt
      661 aaaataaaac tcttttcacg agggagcggg tatggatcta aaatacacag caatggagat
      721 ggtagaggat agcatcgcta tagttaaaat taatcgtcct gatgccttga atactttaaa
      781 tcgggatgta ttgcaggaat tggaagaggt ttttcgtaaa ttgggagatg atgataaagt
      841 gaaagtagtg ataataaccg gcgagggaaa agcgtttgtg gctggagcgg atatagctca
      901 aatgaaggat atgagcggaa tgcaggcaag ggaattttcc aaactaggac aacaggtttt
      961 tggcctgatt gagtccctgg agaagcctgt tatcgcagca gttaatggtt ttgctttggg
     1021 cggaggttcc gaattggctt tggcttgcga tattcgtatc gcttcagata aagcaaaatt
     1081 ggggcagccg gaggttaact taggtataat ccctggcttt gctggcacac agaggttgag
     1141 tcgcttaatc ggacccagta atgcgaaaat gctaatatac accggcgaca tcattgatgc
     1201 ccagaccgct ctacattatg gtttggtcaa caaagttgtt ccgggtgaag aattgatgaa
     1261 ggaggctctt gagttggctc gtaagataat ctccaaaggg ccgaatgcta tcaaattcgc
     1321 taagggggtg ataaataaag gaattcaagc ggatttattg accggcagct cctatgagat
     1381 ggaagcattc agtttatgtt tttcaactgg cgaagccaag gaagggatgt cagcttttct
     1441 ggaaaagagg aagccgaatt ggaaataaaa cagtttcaag ttttgataga acctctccct
     1501 aaccctctcc ttatcaagga aaggggagga gtgggtttcc tctccttttt aaggagagga
     1561 caaaggtgag gttggaagaa cagcgcgtag gggcgaagct tccttcgact ttgctcagga
     1621 acggtgagcc tgtcgaaccg tgacttcgcc cgaagggtgc gggtggagat gaactccacc
     1681 cctacgcgag cgtaaaaata gatatgtagg cgacattcgc gaatgtcgcc tacaagagaa
     1741 aatataaacc gggtaagtaa tggctgtgcc attacttctt ggggaacggc acagccgttc
     1801 cctacccctt aaagcatggt tggttcagga attgggtttc ccgaaccata aagtcaaaag
     1861 gttgtgcagg aattccaatt tctgcaaaaa cgaaaaattt caggtcaagc taaagcttga
     1921 cactccataa aacctttttt ctgtccgact tttggcaatc tcataaaatc aggacaggga
     1981 gataacaaaa caatctggaa gaataataaa tggagatttt caaagagtta gcaattgaca
     2041 aacatgagca agttgttttc tgctcggata aggtctctgg tttgcgttcc ataattgcca
     2101 ttcacgacac cactttaggc ccagctatcg gcggagtcag gatgtaccct tacaaatctg
     2161 aagatgatgc cttaaaggat gtgctcttgc tttccaaggc gatgacttat aaagcggcag
     2221 cagccggggt taatctgggt ggaggtcaag gggtgattat gggaaaccca gacaaagaca
     2281 aaaccgaaat tctattgagg actttcggca gattcgttaa tggtctaaac ggccgctata
     2341 tcacggctga ggatttgggc accacagtag aggatatgga gatagttcac tatgaaacca
     2401 aatatgtggc tggactgtct cgcgctgtgg gaggtggagg agatcccggt ccggtctcag
     2461 ctattggagt ctattatgga attatagctt gtgtagaaaa agtttttaag gataaagacc
     2521 tctccaaaag atcgatagtt gttcagggtt tggggaatgt gggatacagc ttagcccaga
     2581 aattagcaga tgagggggcg aagctttttg tggcggatgt gattgaatct aaagttgaga
     2641 gggcaactaa agaattcaaa gccgaggtgg ttaacccgga tgaagtattt gaaatagaag
     2701 ctgatatttt ttctccctgt gctttaggag gagtgataaa taaaactaac attgataaga
     2761 tgaaagcgaa gat
//
LOCUS       DASWLN010000108         1867 bp    DNA     linear   ENV 20-FEB-2024
DEFINITION  MAG TPA_asm: Candidatus Zixiibacteriota bacterium isolate
            SMAG_U2949 k141_606538, whole genome shotgun sequence.
ACCESSION   DASWLN010000108 DASWLN010000000
VERSION     DASWLN010000108.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37960594
            Sequence Read Archive: SRR10376091
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Candidatus Zixiibacteriota bacterium (soil metagenome)
  ORGANISM  Candidatus Zixiibacteriota bacterium
            Bacteria.
REFERENCE   1  (bases 1 to 1867)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 1867)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (26-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/12/2024 08:28:26
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 1,440
            CDSs (total)                      :: 1,416
            Genes (coding)                    :: 1,412
            CDSs (with protein)               :: 1,412
            Genes (RNA)                       :: 24
            rRNAs                             :: 1 (16S)
            partial rRNAs                     :: 1 (16S)
            tRNAs                             :: 21
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 4
            CDSs (without protein)            :: 4
            Pseudo Genes (ambiguous residues) :: 0 of 4
            Pseudo Genes (frameshifted)       :: 0 of 4
            Pseudo Genes (incomplete)         :: 3 of 4
            Pseudo Genes (internal stop)      :: 1 of 4
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..1867
                     /organism="Candidatus Zixiibacteriota bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_606538"
                     /isolate="SMAG_U2949"
                     /isolation_source="soil"
                     /db_xref="taxon:2053527"
                     /environmental_sample
                     /country="USA: Oregon"
                     /lat_lon="42.99 N 123.63 W"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            532..1494
                     /locus_tag="VGB16_03675"
     CDS             532..1494
                     /locus_tag="VGB16_03675"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_013809852.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_process: GO:0006810 - transport [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="sulfite exporter TauE/SafE family protein"
                     /protein_id="HEX9916814.1"
                     /translation="MLFPISGVKTYIFIPPLVALVVSFFTSMGGVSGAFLLLPFQMSF
                     LNFTSPSVSATNFVFNIVAIPSGVYRYLKEGRMAWPLTWVVIAGTLPGVFIGYYLRVL
                     YLPNPRTFKLFVGCVLLYIGVRLIYEITGKAKAGKVKMKALEDKFKERAKQIKEQQKS
                     RVASGLPPEAVVKAVSASFTRIEYEFWGERFSYSTIAMFLLAFFVGIIGGTYGIGGGA
                     IIAPFCVAVFHLPVYTIAGAALMGTLITSVAGVIFYSIIPAKSGMVTSPDWLLGFLFG
                     IGGFVGIYLGARFQKFVPQKVIKLMLGTIIVFLALKYISQFFMK"
BASE COUNT          526 a          369 c          416 g          556 t
ORIGIN      
        1 gatgtggtcg accattcttg gttcctgatt taccaaaaaa caccaatcgt cgtttgaaat
       61 cgatatcatg gaagggatta aaagctaata cttcgttcag cttggcaccg gtatagcgta
      121 tcaaaaggaa tatcagcagg atacgtttac gggataaacg aatgtcagcc cgtgacgatc
      181 cctccgacca gtttcggaat gactgctcca ttcgattcag ctgaaccgta tcaagacatt
      241 ggacttcgtt tggaatcgag acaatacgcg cgtggcttga tcgctcagtt ataaaagagt
      301 acggcacttt ttgacccttc tgaaacatta ctcctttatt agagcgccta cccacagctt
      361 ttttccgatc cattcatggc taccataatt ctaatatata ataaattttc tgcaaagctc
      421 ttgacaagca gatatacaac atgatagagt tttaatcaac acgaaaagta taattatcgt
      481 gttaattagt aatttttaat ttttaaagga actttcatgg attttctcta tatgctcttt
      541 cctatatcgg gggtgaagac atatatattt atcccgcccc tcgttgcact cgttgtctct
      601 ttcttcacct ctatgggcgg tgtctcaggt gcatttctgt tgttaccatt ccagatgagt
      661 tttcttaatt tcacaagtcc ttccgtaagt gctacaaatt tcgtcttcaa tatcgtcgca
      721 atcccgagcg gtgtttaccg ctatctcaag gaaggccgca tggcgtggcc tctgacctgg
      781 gttgtcattg caggtacact ccctggtgtc tttataggat actatctccg ggttttgtat
      841 ctgcccaatc cgagaacgtt taaactcttc gttgggtgtg tgctccttta tatcggtgtg
      901 cgccttattt acgaaattac aggcaaggcg aaagcaggaa aagtaaaaat gaaggccctt
      961 gaagataaat tcaaggaaag ggcaaaacag attaaggaac aacaaaaaag cagggtagcc
     1021 tccggtctcc ctccagaggc tgtcgtcaaa gcagtctcag ccagttttac gaggatagaa
     1081 tatgagttct ggggagaaag gttctcatac agcacaatag ccatgtttct tcttgccttt
     1141 tttgtaggta tcattggcgg cacttacggg attggtggag gagcgattat cgcaccattc
     1201 tgcgttgcgg tattccatct gcccgtttat acaattgcag gggctgcctt aatggggaca
     1261 ttaataactt cagttgcagg ggtaattttc tacagtatca ttcctgcaaa gagtggaatg
     1321 gtgacttcac cggactggct ccttggattc ctctttggca taggagggtt tgttggcata
     1381 tatctcggtg cgcgatttca aaaattcgtt ccccagaagg ttattaaact catgctgggg
     1441 acaattattg ttttcctcgc attgaagtat atttcgcagt tttttatgaa ataaatgttt
     1501 agaataaatg gagtaatagc tatcaaaatg aagttactaa tgagttcatt agtaaagcct
     1561 tataattctg tcattcctgc gaaagcagga atccagggaa agcaaagaaa aactggattc
     1621 ccgcttaagt actgcgggaa tgacagaaca agaataactg aattgttact gaaaggatag
     1681 agtgttggcg tcaaggatat ttacggtagg aggccactaa tatgaacgga caggtatgcc
     1741 cgatagcaaa aatggagaag ctgcttgtag caacagacgg atctgtgttc agcaaaagcg
     1801 ctgtaagaga ggctgtaaat cttgcaaaga tatgttcagg aaaattatat gtggtatcag
     1861 tagttaa
//
LOCUS       DASWLN010000109         5783 bp    DNA     linear   ENV 20-FEB-2024
DEFINITION  MAG TPA_asm: Candidatus Zixiibacteriota bacterium isolate
            SMAG_U2949 k141_986361, whole genome shotgun sequence.
ACCESSION   DASWLN010000109 DASWLN010000000
VERSION     DASWLN010000109.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37960594
            Sequence Read Archive: SRR10376091
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Candidatus Zixiibacteriota bacterium (soil metagenome)
  ORGANISM  Candidatus Zixiibacteriota bacterium
            Bacteria.
REFERENCE   1  (bases 1 to 5783)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 5783)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (26-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/12/2024 08:28:26
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 1,440
            CDSs (total)                      :: 1,416
            Genes (coding)                    :: 1,412
            CDSs (with protein)               :: 1,412
            Genes (RNA)                       :: 24
            rRNAs                             :: 1 (16S)
            partial rRNAs                     :: 1 (16S)
            tRNAs                             :: 21
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 4
            CDSs (without protein)            :: 4
            Pseudo Genes (ambiguous residues) :: 0 of 4
            Pseudo Genes (frameshifted)       :: 0 of 4
            Pseudo Genes (incomplete)         :: 3 of 4
            Pseudo Genes (internal stop)      :: 1 of 4
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..5783
                     /organism="Candidatus Zixiibacteriota bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_986361"
                     /isolate="SMAG_U2949"
                     /isolation_source="soil"
                     /db_xref="taxon:2053527"
                     /environmental_sample
                     /country="USA: Oregon"
                     /lat_lon="42.99 N 123.63 W"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            complement(<1..815)
                     /locus_tag="VGB16_03680"
     CDS             complement(<1..815)
                     /locus_tag="VGB16_03680"
                     /inference="COORDINATES: protein motif:HMM:NF024816.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="tetratricopeptide repeat protein"
                     /protein_id="HEX9916815.1"
                     /translation="MAQYEIGTIEEYWGNIEEAKRRYLEALKEVDDYHDIHFRLGKIY
                     FGEKDYISAKEELEKYLNKEKKVATAYNFLGDCHYNLGDYPKALEYYRQSFEVDETFL
                     DGMYNAGLVAEKLCNFSLSLEIFNKLIANEPKYKDGYRQRGDLYFQLSDFECASVDYQ
                     HFLEFNPKDAEVMSNLGNSYLRMNNLDKAEECYRKAMELNPGFSLAHRNLGVVLKVKG
                     KSKESISVLQGYLELEPNDFEVCLHLADSLYELKDYKTALSWYEKYMTRCPSD"
     gene            complement(879..2669)
                     /locus_tag="VGB16_03685"
     CDS             complement(879..2669)
                     /locus_tag="VGB16_03685"
                     /inference="COORDINATES: protein motif:HMM:NF012745.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="tetratricopeptide repeat protein"
                     /protein_id="HEX9916816.1"
                     /translation="MGKKKGKKEFLQNNHNHLLPLEQWLLKLENSFAQGKFNVVIEVA
                     NKMLKVYNECLDNRIIGRIYYLLAFSRAQSGDYYGSKSLCLEMVPHRIETLDLYYILS
                     FACCRLQEYNRCIEYSRRFLELKNKLATHNKVDKLLSTTLEAEHEVHNNLGIACREMG
                     DVEEGIRMFEKSLELKPDYHIARINLALSYGQMDEIDKGIQVIKEGIDKGYKDVNLYN
                     ILGVLYTEAKDWVKAEGIYNYTIKEFGEHVEVYSNLGVLYNKMGKQDEAAKYCQKAIQ
                     LNPQHEIASDNLSALKKTYADKPETISLCMIVKDEEDCLARCLESVKDVVDEIIVVDT
                     GSTDNTVKIAKKFGAKVFHHPWEGDFSKARNYSIGYATCDWILYLDADEELFVEDKLK
                     LKKVTKEKDCNGISFLIYNKVYGQRQGFLYYTRMWRNRIGAYFEGIVHNQLVFPGSVL
                     KSDIRIMHYGYALEDQEKMEKKQKRSKELLKKQIKEDPNNVFARFNYAQICRGQRLYD
                     EVIEHASKVIEILTPQDKLKLHIYLMALGQLSNAYFDRRDYGKCLEYCQKALDLKPDY
                     LDPLFMIGGACKLLGNLEKAKESLEKYLEV"
     gene            complement(2673..3113)
                     /locus_tag="VGB16_03690"
     CDS             complement(2673..3113)
                     /locus_tag="VGB16_03690"
                     /inference="COORDINATES: protein motif:HMM:NF024914.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="glycosyltransferase"
                     /protein_id="HEX9916817.1"
                     /translation="MRHLLISNANFGLKSDLPFTQLPNTETRTFEVLGSGTFCLTEEL
                     SMPEMFEESKHLVSWRDKENLLEKIRYYLENKEEREEVALQGMDFALEHHTYLHRAKE
                     VLKKVKNVKMHPADFKFGVMYDKDGEITLSADEYYEAVSQMIRE"
     gene            complement(3525..3893)
                     /gene="csrA"
                     /locus_tag="VGB16_03695"
     CDS             complement(3525..3893)
                     /gene="csrA"
                     /locus_tag="VGB16_03695"
                     /inference="COORDINATES: protein motif:HMM:TIGR00202.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003723 - RNA binding [Evidence IEA];
                     GO_process: GO:0006109 - regulation of carbohydrate
                     metabolic process [Evidence IEA];
                     GO_process: GO:0006402 - mRNA catabolic process [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="carbon storage regulator CsrA"
                     /protein_id="HEX9916818.1"
                     /translation="MLILTRKLGQSVTIGDDVKVTVLDIHGGQVKLGITAPHKVTVHR
                     EEVYQKIQEENKRAAMANSNDLEQAKKLIGFPSHPVRSPSTQTPASGDVRQHKAYGVE
                     KVKKTDPVVDKDNKERSSNL"
     gene            complement(3914..5308)
                     /gene="flgK"
                     /locus_tag="VGB16_03700"
     CDS             complement(3914..5308)
                     /gene="flgK"
                     /locus_tag="VGB16_03700"
                     /inference="COORDINATES: protein motif:HMM:TIGR02492.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0009424 - bacterial-type flagellum hook
                     [Evidence IEA];
                     GO_function: GO:0005198 - structural molecule activity
                     [Evidence IEA];
                     GO_process: GO:0001539 - cilium or flagellum-dependent
                     cell motility [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="flagellar hook-associated protein FlgK"
                     /protein_id="HEX9916819.1"
                     /translation="MYGILSGFEIGKRALLSQQFGLNVTGHNIANVNTPGFSRQKAII
                     NTTHPQLSIKGALGTGVDAAGVSRYRDIFYDSQYRQENQNLGRWSALQRTFEQLETVF
                     NEPSDQGLNTLMDNFWNAWQDLSTNPESRAAQSTLKEQSLVLVNAFHQQHKLLKDLEQ
                     NINDDIAQKVVEVNNYADQIASLNRQIAYAELSGGKANDLRDKRDLLVDELSKVVDVN
                     TVEQSAGSVTVYVGAMSLVERDIVNRLSADKNYEEGVMRSKVVWSGTLSTVIFNNGEL
                     AGLCQIRDELIPEYVDALNQMAETLVSGVNTVHQQGYALDGTTTGIDFLDPERTTAQD
                     IALSSDILSNLGNIAASQGGAPGNNANALAIAALRSSLLMNDSTATLGDFYSSLIGIL
                     GSRSQEAEDIKDSQNLLLAQIKNGRQAVQDVSLDEETTNLIRFQHAYEAAVRVITTMD
                     EVMATIINSVGKVA"
     gene            complement(5437..>5783)
                     /gene="flgN"
                     /locus_tag="VGB16_03705"
     CDS             complement(5437..>5783)
                     /gene="flgN"
                     /locus_tag="VGB16_03705"
                     /inference="COORDINATES: protein motif:HMM:NF016985.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005515 - protein binding [Evidence IEA];
                     GO_process: GO:0044780 - bacterial-type flagellum assembly
                     [Evidence IEA]"
                     /codon_start=3
                     /transl_table=11
                     /product="flagellar export chaperone FlgN"
                     /protein_id="HEX9916820.1"
                     /translation="LEAEKLEKERIKLTDQIASNLKLDKDEASLSRLIQLLEESYSTQ
                     LQELQKTLLYLYQKVERQRRKNEFLIRQSMGMVDRNMKFILGVEAAAPTYPRPNGKKA
                     QAYGRKVVDRLG"
BASE COUNT         1439 a         1469 c         1061 g         1814 t
ORIGIN      
        1 taatccgaag gacagcgggt catatatttt tcgtaccagc ttaaggctgt cttgtaatct
       61 tttagctcgt ataagctgtc agccagatgc aagcagactt caaaatcgtt cggttccaat
      121 tccagatatc cctgcagaac gcttatcgat tcttttgact ttccctttac ttttaaaact
      181 acacctaagt tgcgatgggc tagcgaaaag ccaggattta actccattgc ttttcggtag
      241 cattcctccg ccttatctaa gttattcatc cttaggtaac tgtttcccaa attgctcatt
      301 acctctgcat ccttggggtt gaattccagg aaatgttggt agtcaactga agcgcactca
      361 aaatcggaaa gttgaaaata gaggtccccc ctctgccggt atccatcctt gtattttggc
      421 tcgttggcaa tcaacttgtt gaatatctcc agggataaag aaaaattaca caacttctct
      481 gccaccaagc cggcattgta catcccatcc aaaaatgttt catccacttc aaagctctgg
      541 cgatagtact ccagagcctt aggatagtcg cccaaattgt aatggcaatc tcccaagaag
      601 ttataggctg tggcgacctt cttttctttg ttcagatact tctccaactc ctctttggct
      661 gagatataat ctttttcccc gaagtagatt ttccccaagc ggaagtgaat gtcatgataa
      721 tcatccacct ccttcagagc ctccagatac cttctctttg cctcttcaat gtttccccaa
      781 tactcctcaa ttgttcctat ctcatactga gccataaaac ggctctctaa gttaattaga
      841 atgaggttta atacctcctt ggattcatca tatttctcct acacctctaa atatttctcc
      901 aaactctcct tggctttttc taaattcccc aaaagcttgc aagctccgcc tatcatgaaa
      961 agcgggtcca aataatccgg cttaaggtcc agcgcctttt ggcaatattc aagacatttt
     1021 ccataatccc ttctgtcgaa ataagcattg gaaagctggc ccaaagccat gagatagata
     1081 tgcagtttca atttatcctg tggagttaag atttcaatca ccttgctggc atgctcaatg
     1141 acctcgtcgt aaagcctttg tccccgacat atctgggcgt aattgaatcg agcgaaaacg
     1201 ttattcgggt cctccttgat ctgttttttc aaaagctcct tgcttctctt ttgcttcttt
     1261 tccattttct cctggtcttc taaggcgtaa ccatagtgca taatgcggat atcacttttc
     1321 agcacacttc ccggaaagac caactgatta tgcacaatcc cttcaaagta agcgccaatc
     1381 cgatttctcc acatacgtgt gtaatagaga aatccttgcc tctgtccata aaccttattg
     1441 taaatcagaa aggagattcc attgcaatcc ttttccttgg tgaccttctt gagcttgagc
     1501 ttatcttcaa caaagagttc ctcatctgca tccagataca gaatccaatc gcaggtagca
     1561 taaccgatag agtagtttcg agctttgctg aaatctccct cccagggatg atgaaaaacc
     1621 ttagctccga actttttagc tatcttcacg gtattatcag ttgagccggt atccactacg
     1681 attatctcat ccactacatc tttaacgctc tccaaacagc gggccagaca atcctcctca
     1741 tccttgacta tcatacataa ggagatagtc tcaggtttat ctgcatacgt tttcttcaaa
     1801 gcagacaaat tatcgctggc gatttcatgt tggggattaa gctggatcgc cttttggcag
     1861 tattttgcgg cttcatcttg ttttcccatc ttgttgtata gtactcctaa gttcgaatag
     1921 acctcaacat gttccccaaa ctcttttatg gtatagttat agattccctc ggctttgacc
     1981 caatctttgg cttcggtgta aagcactccc agaatgttat acaggttgac atccttatat
     2041 cctttatcta taccttcttt tattacttga atccccttgt ctatctcatc catttggcca
     2101 taactcaaag ccaaattgat tcgagcaata tgataatccg gctttaattc caaggacttc
     2161 tcgaacattc ttataccttc ttccacatcc cccatttccc gacaggcaat tcccaaattg
     2221 ttgtggactt catgttctgc ttcaagggtg gtgcttaaaa gcttgtccac cttgttgtgt
     2281 gtagccaact tgtttttcaa ctccagaaac cttcgactgt attctataca ccggttatat
     2341 tcttgcaagc gacagcaggc aaagctcaag atatagtaaa gatcgagcgt ttctatccta
     2401 tggggaacca tctccagaca taagctctta gaaccatagt aatctccact ttgagctcgg
     2461 gaaaaagcca gaagataata tatgcggccg attatccgat tatccagaca ttcattatag
     2521 accttgagca ttttgttggc aacttcaatg accacattga acttcccttg cgcaaaactg
     2581 ttttccagtt tcagaagcca ttgctccaat ggaagcaaat ggttgtggtt gttctggagg
     2641 aattcctttt ttcctttctt cttccccatc ttctattccc ttatcatttg tgaaactgcc
     2701 tcgtaatatt catccgcgga caaagttatc tcaccatctt tatcatacat cactccaaac
     2761 ttgaaatccg ccggatgcat tttgacattt ttgaccttct tcaaaacctc tttagctcga
     2821 tgtagatagg tatgatgttc taatgcgaaa tccataccct gcagagctac ttcttctctt
     2881 tcctctttat tctccaaata atatcttatc ttctccagca aattctcttt gtctctccag
     2941 ctaactaagt gcttactttc ctcaaacatc tctggcatag aaagctcttc ggttaaacaa
     3001 aaagtgcctg agcccaaaac ttcaaatgtg cgagtttcgg tattgggtag ctgagtgaaa
     3061 gggagatcag attttaaccc aaaatttgcg ttagaaatca gaaggtgtcg caacttatta
     3121 ttatcccaag ttcggggata acctcggcat tcctgattga atctcccccg agatttaatc
     3181 agccgaccct acgccctctg tcccgccctg gccggaagag gattgagtcc cggcccctct
     3241 acattcttac agaatgtaag aagaggggct cgggagggag attttactaa tgcaaaatct
     3301 ggatttaaat gacgaagcaa aaggggttat cactcttcgt aatagacgaa gctatcgtct
     3361 atcattctgc ccttagtgaa agaggacccc ttctcttttt ggattgagga accattcacg
     3421 tctttttggt ccctccacca ctttttgtag cgcgaaactt tataattaag ttcccgcata
     3481 ggagttcggc gatctttgat tttagagaac atgattagtt tgggttatag gtttgaactt
     3541 ctttccttat tatccttatc gactactggg tcggtttttt taactttttc cactccatac
     3601 gccttatgtt gtcttacatc gcctgaagcg ggagtctggg ttgaagggct acgcacagga
     3661 tgtgacggaa acccgattaa ttttttcgct tgctctaaat cattgctgtt tgccatagcc
     3721 gccctcttgt tctcttcttg aatcttctgg taaacctcct cacgatgaac tgttacttta
     3781 tggggagcgg ttatccccaa tttcacctgt ccgccatgaa tatccaagac cgtaactttg
     3841 acgtcatctc cgatggtaac gctctgcccc aacttccgtg tcagaattaa catctatttt
     3901 tcctcgttca atcctaagcc actttaccta cactgttgat tatagtagcc atcacttcgt
     3961 ccatagtagt tatcactcgc accgccgcct cataggcatg ctgaaaacga atcagattgg
     4021 tagtctcttc atccagagag acgtcttgca ctgcttgacg accgttctta atttgagcaa
     4081 gtagaaggtt ctggctatct tttatatcct ccgcctcctg actgcggcta cccaagattc
     4141 caatcaggga ggagtaaaaa tctcccaggg tggcagtgct atcgttcatt aatagactgc
     4201 tgcgtaaggc ggcaatggct aaggcgttgg cattattccc gggcgccccc ccctgcgaag
     4261 cagcgatgtt acctaaattg cttaagatat ctgaactcag ggctatgtct tgggcggtcg
     4321 tccgttcggg gtcaaggaaa tctatcccgg tggtggttcc atctaaggca taaccctgtt
     4381 ggtgaacggt gttcactcca ctcaccagag tctccgccat ctgatttaaa gcatcaacat
     4441 actccggaat caattcatct ctgatttgac ataacccagc cagctcccca ttattaaata
     4501 tgacagtgct gagagtcccg ctccaaacca ctttgcttct catcacccct tcttcgtaat
     4561 tcttgtccgc gctaaggcga tttactatat ccctctccac cagcgacata gctccgacat
     4621 agaccgtaac tgaaccggcg ctttgctcta cggtatttac atccacgacc ttagaaagct
     4681 catccactag tagatcgcgc ttatccctca agtcattggc tttccctcca ctcaattcag
     4741 cataagcgat ctgtcgattc aggctggcta tttggtcagc atagttattc acctccacca
     4801 ccttttgagc gatgtcatca tttatgttct gttccaaatc ttttagaagt ttatgctgtt
     4861 ggtgaaaggc atttactaaa accaagctct gctccttcaa agttgattgg gcagcccggc
     4921 tttccggatt ggtggataga tcctgccaag cgttccagaa attatccata agcgtattta
     4981 atccttgatc cgaaggctca ttaaacaccg tctccagctg ttcgaaagtc cgttgcaaag
     5041 cagaccagcg tcccagattc tggttttcct ggcgatattg actgtcatag aagatgtctc
     5101 gataacgact tactccagcc gcatccactc cagtacccaa agctcctttg atgctcaact
     5161 gaggatgagt ggtatttatg atagctttct gccgcgagaa tccgggagtg ttcacattag
     5221 cgatattgtg accagttacg ttcaatccaa attgctggga taagagtgct ctcttgccga
     5281 tttcaaaacc actcagtatt ccgtacatct tccctcctga aaaattttaa tcgggtaagt
     5341 aatggctgcg ccattacttc tgctgtcttt tatctcttcg ggaacggtgc aaccgttccc
     5401 tacccgtttc cttatagtaa gctataaaag tttatcttaa cctaatcggt ccacaacctt
     5461 gcggccataa gcttgtgctt tcttgccatt gggcctggga taggtcggcg cagccgcctc
     5521 cacccccaag atgaacttca tgttacgatc taccattccc attgattgcc ttatcaagaa
     5581 ctcatttttc cttctttgtc tttccacttt ttggtaaaga taaagtaatg tcttctgcaa
     5641 ttcctgcagt tgagtagaat aggactcctc caaaagctga atcaatcgag agaggctggc
     5701 ttcgtcctta tccagtttga gattcgaggc tatctggtct gtcagtttaa tcctttcctt
     5761 ctccaatttt tccgcctcga gta
//
LOCUS       DASWLN010000110         1797 bp    DNA     linear   ENV 20-FEB-2024
DEFINITION  MAG TPA_asm: Candidatus Zixiibacteriota bacterium isolate
            SMAG_U2949 k141_350407, whole genome shotgun sequence.
ACCESSION   DASWLN010000110 DASWLN010000000
VERSION     DASWLN010000110.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37960594
            Sequence Read Archive: SRR10376091
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Candidatus Zixiibacteriota bacterium (soil metagenome)
  ORGANISM  Candidatus Zixiibacteriota bacterium
            Bacteria.
REFERENCE   1  (bases 1 to 1797)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 1797)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (26-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/12/2024 08:28:26
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 1,440
            CDSs (total)                      :: 1,416
            Genes (coding)                    :: 1,412
            CDSs (with protein)               :: 1,412
            Genes (RNA)                       :: 24
            rRNAs                             :: 1 (16S)
            partial rRNAs                     :: 1 (16S)
            tRNAs                             :: 21
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 4
            CDSs (without protein)            :: 4
            Pseudo Genes (ambiguous residues) :: 0 of 4
            Pseudo Genes (frameshifted)       :: 0 of 4
            Pseudo Genes (incomplete)         :: 3 of 4
            Pseudo Genes (internal stop)      :: 1 of 4
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..1797
                     /organism="Candidatus Zixiibacteriota bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_350407"
                     /isolate="SMAG_U2949"
                     /isolation_source="soil"
                     /db_xref="taxon:2053527"
                     /environmental_sample
                     /country="USA: Oregon"
                     /lat_lon="42.99 N 123.63 W"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            <1..189
                     /locus_tag="VGB16_03710"
     CDS             <1..189
                     /locus_tag="VGB16_03710"
                     /inference="COORDINATES: protein motif:HMM:NF012708.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0051219 - phosphoprotein binding [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="FHA domain-containing protein"
                     /protein_id="HEX9916821.1"
                     /translation="ISRHHARMTKEGNSYFIEDIGSEGKGSTNGTYIGDKKITRKTPL
                     PSGDTVLLGGVWVRFEAK"
     gene            176..1531
                     /locus_tag="VGB16_03715"
     CDS             176..1531
                     /locus_tag="VGB16_03715"
                     /EC_number="2.7.11.1"
                     /inference="COORDINATES: protein motif:HMM:NF012298.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004674 - protein serine/threonine kinase
                     activity [Evidence IEA];
                     GO_function: GO:0005515 - protein binding [Evidence IEA];
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_process: GO:0006468 - protein phosphorylation [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="serine/threonine-protein kinase"
                     /protein_id="HEX9916822.1"
                     /translation="MRQSKNEAIAFHLGIQTMGIFDSFKKKKPGKSSSEAKQETSARP
                     QEATSGRWHVGDRIDNRYQIYRILGGPGKSGMGIVYVCYDHRDMVPVAIKTLQDQFLR
                     DHNLIERFKREAETWVRIEKHYNIVQANYVQEIDGRPYIFLEYIVGDEKYGADLSGWI
                     WKGGLTIPLTLNFAIQFCHGMIHADKKFREMGKTFVHRDVKPSNIMITQDKIVKITDF
                     GLVRAFAESDDDMSSLTFGDESHPRLGLSKSGNICGTPPYMSPEQCRGEKDIDARSDI
                     YSFGCVLYEMVTRKYVFDVRTLDEFMYHHLNTVPKSPNVQKELNRLVMKCLEKDPSKR
                     YQGFNELEEVLFELYHRLTGKVVKQPEAATLVAGEVYNKGFSLANLGLHEEAIGFYVK
                     VLQLNPNDAEVWNGKASALAEIGKYQESMDCCDKALEIDPKHTGAWYNKGFSLENLGH
                     I"
     gene            1586..>1797
                     /locus_tag="VGB16_03720"
     CDS             1586..>1797
                     /locus_tag="VGB16_03720"
                     /inference="COORDINATES: protein motif:HMM:NF024816.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="tetratricopeptide repeat protein"
                     /protein_id="HEX9916823.1"
                     /translation="MWKNKGDSLNSLGRYKEAIECYDKALELDPQNVEAWNCKGNSLG
                     YSGKDKDALACYNRALEINPNIDFVW"
BASE COUNT          561 a          333 c          455 g          448 t
ORIGIN      
        1 atttcaagac accatgcaag aatgaccaaa gaaggtaatt cctattttat tgaggatata
       61 ggatcagagg gaaaagggag caccaacggc acttacattg gcgataagaa aattactagg
      121 aaaacgcctc taccatccgg cgacacggtt ctactggggg gcgtctgggt cagatttgag
      181 gcaaagtaaa aacgaggcaa tagcattcca cctagggatt caaactatgg gtatctttga
      241 tagctttaaa aagaaaaagc ctggtaagag ttcaagcgaa gcaaagcagg agacttctgc
      301 cagaccacaa gaggcaacct ccggaaggtg gcacgtaggg gataggattg ataaccgcta
      361 tcagatttac cggattctgg gcggaccggg caaaagcggt atgggaattg tttacgtctg
      421 ctatgatcac cgagacatgg tacccgtggc tattaagact ctccaagatc aattcttaag
      481 agaccataac cttatagagc gtttcaagag ggaggctgaa acctgggtaa gaatagagaa
      541 acattacaat attgttcagg caaattatgt gcaggaaatt gacggaaggc cttatatctt
      601 tcttgaatat attgtaggcg atgagaaata cggggctgac ctttctggct ggatctggaa
      661 aggtgggctt accattccgt taacccttaa tttcgccatt cagttctgcc acgggatgat
      721 tcacgccgac aaaaaattca gagagatggg aaagactttt gttcatcggg atgtcaaacc
      781 gtccaatatc atgataaccc aggacaagat agtcaaaatt accgactttg gactggtcag
      841 agcatttgct gagtcagatg atgacatgtc ctcgctaacc tttggtgacg aatctcaccc
      901 gaggctggga ctgagcaagt cgggtaatat atgtggtacg cccccctata tgtctccgga
      961 gcaatgccgt ggtgaaaaag atatcgatgc gaggtccgat atctacagct ttggatgtgt
     1021 actctatgag atggttacca gaaaatatgt gtttgatgta aggacactag atgaatttat
     1081 gtatcatcac cttaatactg tacctaaatc gccgaacgtt caaaaagaac tgaaccggct
     1141 ggtaatgaaa tgtctagaga aagaccctag taaaaggtat cagggcttca acgaactaga
     1201 ggaggttctc ttcgaacttt accaccgatt gaccggaaaa gtggtcaagc agccagaggc
     1261 agcaacactt gtggctgggg aagtatataa caagggattt tccttagcca acctgggact
     1321 tcacgaagaa gctatcggtt tttacgtaaa agttctgcaa ctcaacccaa atgatgccga
     1381 ggtttggaat ggtaaagcaa gcgctttggc ggagattgga aagtatcagg aatccatgga
     1441 ttgttgtgac aaagctcttg agattgatcc aaagcatact ggtgcttggt ataacaaggg
     1501 gttttctctg gagaatctag gacatatttg agaggccgtt gaatgctata acaaggctct
     1561 gaagattgac ccaagtaccg actttgtgtg gaaaaataaa ggggattctt tgaacagttt
     1621 ggggaggtat aaggaggcaa ttgagtgcta cgataaagca ctagaactag accctcaaaa
     1681 cgttgaagct tggaattgta aaggtaattc attaggatat tcaggaaaag acaaggatgc
     1741 tttagcttgt tataacaggg ctctggagat aaatccaaat atagattttg tctggtg
//
LOCUS       DASWLN010000111        10769 bp    DNA     linear   ENV 20-FEB-2024
DEFINITION  MAG TPA_asm: Candidatus Zixiibacteriota bacterium isolate
            SMAG_U2949 k141_480514, whole genome shotgun sequence.
ACCESSION   DASWLN010000111 DASWLN010000000
VERSION     DASWLN010000111.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37960594
            Sequence Read Archive: SRR10376091
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Candidatus Zixiibacteriota bacterium (soil metagenome)
  ORGANISM  Candidatus Zixiibacteriota bacterium
            Bacteria.
REFERENCE   1  (bases 1 to 10769)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 10769)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (26-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/12/2024 08:28:26
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 1,440
            CDSs (total)                      :: 1,416
            Genes (coding)                    :: 1,412
            CDSs (with protein)               :: 1,412
            Genes (RNA)                       :: 24
            rRNAs                             :: 1 (16S)
            partial rRNAs                     :: 1 (16S)
            tRNAs                             :: 21
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 4
            CDSs (without protein)            :: 4
            Pseudo Genes (ambiguous residues) :: 0 of 4
            Pseudo Genes (frameshifted)       :: 0 of 4
            Pseudo Genes (incomplete)         :: 3 of 4
            Pseudo Genes (internal stop)      :: 1 of 4
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..10769
                     /organism="Candidatus Zixiibacteriota bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_480514"
                     /isolate="SMAG_U2949"
                     /isolation_source="soil"
                     /db_xref="taxon:2053527"
                     /environmental_sample
                     /country="USA: Oregon"
                     /lat_lon="42.99 N 123.63 W"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            52..645
                     /gene="nadD"
                     /locus_tag="VGB16_03725"
     CDS             52..645
                     /gene="nadD"
                     /locus_tag="VGB16_03725"
                     /EC_number="2.7.7.18"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_013625606.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016779 - nucleotidyltransferase activity
                     [Evidence IEA];
                     GO_process: GO:0009435 - NAD biosynthetic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="nicotinate-nucleotide adenylyltransferase"
                     /protein_id="HEX9916824.1"
                     /translation="MFKRIGILGGTFDPPHLGHLVLAEELAEELKLDRVLFIPSAVPP
                     HKDISEVSSAKHRFEMTKRAIKGNKLFSVSDIELKRKGPSYTVDTIRELKQIYPQSEL
                     FFLTGSDILEEILKWKDPQEICRQIKIVIGLRPGFDKIDPANQFAHKSQIIEITSLNI
                     SSTLIREKIKKGKSVKYLVPSEVLDYIKNNKLYCNPD"
     gene            676..3375
                     /gene="polA"
                     /locus_tag="VGB16_03730"
     CDS             676..3375
                     /gene="polA"
                     /locus_tag="VGB16_03730"
                     /EC_number="2.7.7.7"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_009184895.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003676 - nucleic acid binding [Evidence
                     IEA];
                     GO_function: GO:0003677 - DNA binding [Evidence IEA];
                     GO_function: GO:0003887 - DNA-directed DNA polymerase
                     activity [Evidence IEA];
                     GO_function: GO:0008408 - 3'-5' exonuclease activity
                     [Evidence IEA];
                     GO_process: GO:0006261 - DNA-templated DNA replication
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="DNA polymerase I"
                     /protein_id="HEX9916825.1"
                     /translation="MNRKKLFLIDGSALVYRSYFAFIRNPLINSRGENTSAVFGFANS
                     LLKILREQKPDYIAVVFDTPAPTFRHKIYKDYKSTRAKTPPEMVEQLPRIRELVGYMN
                     LPLIEKDGFEADDIIGTLAEQGKKEGLEVFLVTADKDFFQLVDEDIKMVRPSKGKEPE
                     EIYDAKAVKEKFGIEPNQMTDLLGLMGDSSDNIPGVPGIGEKTALELINQFGSLEDVI
                     AGADRISKKKVSQNLKEYAEQARFSKKLVTIDTAAPFKLDLRELSKKDFDSPKLRQFF
                     KEMEFTSFLRELSSDKKEEEKVIYKTVQSLDELETLIEVLKQKREFAFDTETDSLDYM
                     NANLVGVSICYQPKEAYYLPLSHQTGDKHKNLDKEKALLELKSVLEDEKIKKIGQNLK
                     FDLEVLKNSGVDLRGIYFDAMLASYLLDPSSRQHNLTYLALRYFDHEMIPLKSLIGSG
                     KKEISFDMVPIECASQYSCEDADYTLRLKDLFATQLSELKLDKLFFEVEVPLIEVLAD
                     MEMTGVSIDTAYLKSMSIDLEKDLKRLEKEIYQLAGKEFNINSPQQLGQILFEELKLT
                     PIRKTEKKTGESTDFAVLEELAREHPLPKILLEYRLLSKLKSTYADALPSMVNPKTKR
                     IHTSFNQTVTATGRLSSSEPNLQNIPIKTEMGSRIRKAFIPRNSDYKLLCADYSQIEL
                     RILAHFSKDEKLIESFRKGEDIHSRTASEVFGVPLNEVTPQQRRQAKTANFAVIYGVS
                     PYGLSQQTEMTPKEAKEFIDVYFAQHPQVKEYIDNCIEKARRDGYVTTLLGRRRYIPE
                     ITSRNRQRREFAERTAINTPIQGSAADLIKVVMLDIRNLLTKKKTKSKMILQVHDELV
                     FEVHKEELHFMKDMVRDKMEGCVKLEVPIKVDIGIGENWLETK"
     gene            3357..4049
                     /gene="coaE"
                     /locus_tag="VGB16_03735"
     CDS             3357..4049
                     /gene="coaE"
                     /locus_tag="VGB16_03735"
                     /EC_number="2.7.1.24"
                     /inference="COORDINATES: protein motif:HMM:TIGR00152.1"
                     /note="Dephospho-CoA kinase (CoaE) performs the final step
                     in coenzyme A biosynthesis; Derived by automated
                     computational analysis using gene prediction method:
                     Protein Homology.
                     GO_component: GO:0005737 - cytoplasm [Evidence IEA];
                     GO_function: GO:0004140 - dephospho-CoA kinase activity
                     [Evidence IEA];
                     GO_process: GO:0015937 - coenzyme A biosynthetic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="dephospho-CoA kinase"
                     /protein_id="HEX9916826.1"
                     /translation="MAGDKIDMFLVGITGGIASGKTEVARVFKKLGATVISGDELGQE
                     VVKKNKLVLRRLVRVFGKVILNKNGSLNRKRLGRLAFFSRSNVRKLNSIVHPYLLSSL
                     KRQIKKLERSRDIVAVDAALIVEWGIQKQFDLLILVDSRRKNQIERFCSSRKYSSRIG
                     REIIRCQLPKKSKIIYADLVIANNGNLKQLRDKARQVWSIIFALSRKKKKRTIPKNRT
                     WKEGSVCPNSGS"
     gene            4611..6638
                     /locus_tag="VGB16_03740"
     CDS             4611..6638
                     /locus_tag="VGB16_03740"
                     /EC_number="2.2.1.1"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_018205827.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="transketolase"
                     /protein_id="HEX9916827.1"
                     /translation="MPILDSASGEIRKSYSIEELRQAANLMRGYDLVTLCAANSGHSG
                     GTMSIMDIVAALYLHIARHDPQAPFWEDRDRIIFSTGHKAPCLYLGLGISGYFNIEEV
                     VKLRKLYSPFQGHPHWLKLPGVEASTGSLGQGLSIAVGIALSAKLDKKDYRAYCITGD
                     GEHQEGQIWEAVMEAGHYKLDNLVCVVDKNRLQIDGWVNEVMNIDPLNEKYKSFGWEV
                     IEIDGHNMQEIVFAFEKAKGIKGKPTVIIAHTTKGKGVSFMENVAGWHGKVPICDQLY
                     AALKELGLEGKLDVDKLFAIAEKYQKEVTEKLEAKLPKFKRDYFWNKGEKMKAEMAAT
                     RLGFGKALQKYGDDPRVVCLGLDISGSIAISEFCSKNPERKERFLSMGIAEQSATGVA
                     AGLAKEGKLPVLGTYGVFASGRNWDQLRTTVCYGNFNVFVAGAHGGVSVGADGATHQA
                     LEELFLTCGIPGMNVVVPCDIVETERATKYLLFELAGPKYLRFAREATPIVTTEATPF
                     VFGKANVIRYREEKDNFRDAFETKLASEYKSEKEDLTIIGCGPEVPEAMRAAYILKKD
                     FKIDTRVVNMHTIKPLDKEAIIRAALETGIIVTAEEHQVGGLGDKVSAVVSQAKELYR
                     KPIIMGMIGVKDRFGESGQPWELIKEFEVSGEHIAQKAKELYDFKKGRGNS"
     gene            6663..7826
                     /locus_tag="VGB16_03745"
     CDS             6663..7826
                     /locus_tag="VGB16_03745"
                     /EC_number="2.6.1.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_007779429.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008483 - transaminase activity [Evidence
                     IEA];
                     GO_function: GO:0030170 - pyridoxal phosphate binding
                     [Evidence IEA];
                     GO_process: GO:0009058 - biosynthetic process [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="pyridoxal phosphate-dependent aminotransferase"
                     /protein_id="HEX9916828.1"
                     /translation="MKLAKRMSRLGTETAFEVLARAKKLEAQGKNIIHLEIGEPDFDT
                     PKNITDAAIGALNSGFTHYGPSAGLPELREVIAKYVSKTRNIKVDGENVVVTPGGKPI
                     MFFAILALADEGDEIIYPNPGFPIYESVINFIGAKPIPVKLREENNFRLDVNELKKLV
                     TSKTKMIIINSPQNPTGGILTKDDLGVIAQIAIKNDIMVLADEIYSRMIYDGEHHSIT
                     SFDGMPERTIILDGFSKTYAMTGWRMGYGVMNKELTPHIAKLMTNSNSCTATFTQKAG
                     IEALVGPQDKVEDMVKEFKKRRDVIVKGLNSIPKVSCKTPQGAFYVFPNITKTGKKSK
                     ELADYLLNEGGVAVLAGTSFGEFGEGYLRLSYANSIKNIEEALKRIKEGLAKM"
     gene            8285..9430
                     /gene="alr"
                     /locus_tag="VGB16_03750"
     CDS             8285..9430
                     /gene="alr"
                     /locus_tag="VGB16_03750"
                     /EC_number="5.1.1.1"
                     /inference="COORDINATES: protein motif:HMM:TIGR00492.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008784 - alanine racemase activity
                     [Evidence IEA];
                     GO_process: GO:0009252 - peptidoglycan biosynthetic
                     process [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="alanine racemase"
                     /protein_id="HEX9916829.1"
                     /translation="MRIRFDNEFSRPAYLEVDLDALAYNIKNIRKKIGKKVEFMAVVK
                     ADGYGHGAYQVAKTVLERGVDCLGVAILEEGIKLREKGIKGKIVNLYPEPIGREKYVI
                     EYDIAQVITDYAFALNLSREAKKLDKIAQVYVEVDTGLGRYGMAPENAIKLVKKIYNL
                     KNIRFKGIISQFSTADEKDKNFALKQLSIFKKTVHDLESSGYRIPIKSIANSGAVLDI
                     PQSYFNQVRVGHLIYGLYPSIYTSQSIKVKPVLKWRSKIAFIKEVEKGTPLGYGRSYH
                     TKKKSKIATIPLGYADGYSRLLSNKAQVLIHNKRLSVVGRVCMDAFMVDVSSIPDVKV
                     GDEVTLIGRQGKEEISAHQLGKWTGTFSYEVLSRIGKRVPIVYLKKS"
     gene            9481..>10769
                     /locus_tag="VGB16_03755"
     CDS             9481..>10769
                     /locus_tag="VGB16_03755"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEX9916830.1"
                     /translation="MSKISLFSTLLVILLSVSSSAQDKAPAINVIYPRNGQTLTSPDS
                     AFIFGSVTPKSSLEIDGEDIKVYPNGAFLAFLPIQPGEFVFELEATNKLGIALDSVEV
                     LVPTTSTTIPEDSLAIEKGSMLPDVDMNLQAGDIIQVRFKGTPGGKATFSIEGLVSGL
                     PMVESVSNKEKYWGEEVFGKGDSSSRIQSKGVYTGAYRIKPEDKIENARVVFCLTKEL
                     NDSLKFDSSAKVDKINNNVSLVSWARGRITISQDSIPQVVEFIDSIQIVRAGPGLGYL
                     LLFQPKGIRAIADGQIGEWTRLRLAPDQEGWVKKSSIEFLPSGTPVPRSKVSLIKTLS
                     QQDKVSIIVPLTQKLPFKVEQDSSGLSLSVFGATANTDWIKYDAKDDLIKQITWSEPQ
                     KLVYQVRIDLNQKQQWGYDAYYDNTDLILGINKKPKIG"
BASE COUNT         3339 a         1958 c         2653 g         2819 t
ORIGIN      
        1 gttggtgaag gaagcccaaa aaagaattga aaaattagag gggaaatcgt agtgttcaaa
       61 agaataggta ttttaggtgg gacttttgac ccccctcatt taggtcacct ggttttagct
      121 gaggagttag ccgaagagtt aaagttggat agggtgctct tcattccatc agcagtgcca
      181 ccacacaaag atataagtga agtctcctct gccaaacaca gatttgagat gaccaagaga
      241 gcgataaaag ggaacaagct cttttcggtc tcggatattg agttgaaaag gaaaggacct
      301 tcatatactg ttgacaccat aagagagtta aaacaaattt atccccaatc cgagcttttc
      361 tttttgaccg gctccgatat attggaggag atattgaagt ggaaggaccc ccaggagatt
      421 tgtagacaaa taaagatagt gataggtttg cgtccgggtt ttgataaaat agatcccgca
      481 aatcaattcg cccacaaaag tcagataata gaaatcacct ctttgaatat ctcctcaacc
      541 ctgataaggg agaagataaa gaagggtaag tcggtaaaat atcttgttcc gtcagaagtt
      601 ctcgactaca taaagaacaa caagctctat tgtaacccgg attagttcgg attagctttt
      661 ttggcaatct caagtatgaa tagaaagaaa ctctttctga ttgatggttc agctttagtc
      721 tatcgttcct attttgcctt cattcgcaat ccgctgatta attcccgagg cgagaatacc
      781 agtgcggttt ttggttttgc caactccctt ttgaagattc ttcgggagca gaaaccggat
      841 tacatagcag tggtattcga tactcctgct cccactttcc gtcacaagat atacaaggat
      901 tataaatcaa cccgagctaa aaccccaccc gaaatggtgg agcagcttcc tcgcatcagg
      961 gaattagtgg ggtatatgaa cctcccctta attgaaaaag atgggtttga ggcggatgat
     1021 ataatcggga ccttggccga gcaaggcaag aaggagggct tggaggtgtt tttggttact
     1081 gcagataagg attttttcca gctggtggat gaggatataa aaatggttag accgagcaag
     1141 ggaaaagaac cggaagaaat ctatgatgct aaagccgtta aagagaaatt cggcatagaa
     1201 ccaaatcaga tgaccgacct tttgggcttg atgggagata gttcggacaa catccctggc
     1261 gtcccaggaa taggagaaaa gaccgccctg gagttgataa accagttcgg cagtttggaa
     1321 gatgtgatag ctggggcgga taggatctcc aagaagaagg tcagccagaa tctgaaagag
     1381 tatgcggagc aagccagatt ctccaagaaa ttggtaacca tcgatactgc tgcccctttc
     1441 aagctggatt tgagagaatt atctaagaag gatttcgatt cccccaaatt gaggcagttc
     1501 tttaaagaga tggagttcac ttccttttta agggagttat cctcggataa aaaagaggaa
     1561 gaaaaggtca tctataagac cgttcaatct ctggatgaat tggaaactct aattgaagtc
     1621 ttaaaacaga aacgagaatt tgcatttgat acggaaaccg atagtttgga ttatatgaat
     1681 gcgaatctgg ttggagtctc aatctgttac caaccgaaag aagcatatta ccttcccttg
     1741 tctcaccaaa ctggcgataa acacaagaat ttagataaag agaaagccct gttggagctt
     1801 aaatcggtgt tggaggatga gaagataaaa aagattggtc agaatctcaa attcgatttg
     1861 gaggtattaa agaatagtgg agttgatttg cgagggatat attttgatgc catgctggct
     1921 tcttatcttt tggacccctc ctcccgacag cacaatttga cttatctggc tttgaggtat
     1981 tttgaccatg agatgatacc cttgaaaagc ctaattgggt cgggcaagaa agagataagt
     2041 ttcgatatgg tgccaattga gtgcgctagc caatattctt gcgaggatgc ggactatacc
     2101 ctgaggttaa aggacctctt tgccactcaa ttgagtgaac tgaaattaga taaactgttc
     2161 tttgaagtgg aggtgccttt aattgaggtt ctggcagata tggagatgac tggagtatca
     2221 attgatacag cttatttgaa aagtatgtcc attgacttag agaaagactt aaaaagatta
     2281 gagaaagaga tttatcaatt agccggaaag gagttcaaca taaattctcc tcaacagctg
     2341 ggccagattt tatttgagga gttaaaactt acccccatca ggaaaaccga gaagaaaacc
     2401 ggggaatcaa ctgatttcgc agtattggaa gagttagcca gagaacatcc cttgcctaaa
     2461 atccttttgg aatatcgctt gctctccaaa ttgaaatcga cctatgcgga tgcattgccg
     2521 agtatggtta atcccaagac caaaaggatt catacctcct ttaaccaaac cgtaactgct
     2581 actggcagac tttcctccag tgaacccaac ttgcagaata tccctataaa aactgagatg
     2641 ggaagtagga taagaaaggc atttatccct cggaactcgg attacaagct tctttgtgcg
     2701 gattactccc agatagagtt gaggatatta gctcattttt caaaagatga aaaactgata
     2761 gaatctttca gaaaagggga ggatatccat tcccgcacag ccagcgaggt attcggagtt
     2821 cccctaaacg aagttacccc acagcaacgc aggcaagcca agaccgccaa ttttgcggtg
     2881 atttacggag tcagtcccta cggcctgtcc cagcaaactg agatgacgcc aaaagaagct
     2941 aaggagttta tagatgtcta tttcgcccaa catccccagg taaaagaata tatagacaac
     3001 tgtatagaga aggcaagaag ggatgggtat gttaccactc ttttgggaag gagaagatat
     3061 ataccggaga taaccagtcg aaatcgtcag agaagggagt ttgctgaaag aactgccatc
     3121 aacacgccaa ttcagggttc agccgcggat ttaatcaagg tggttatgct ggatatccgc
     3181 aatcttctta cgaagaagaa aaccaaatcc aaaatgatct tgcaggtgca cgatgaattg
     3241 gtgtttgaag tacacaagga agagttgcat tttatgaaag atatggtaag agataaaatg
     3301 gaaggatgcg ttaaattgga agtgcccatc aaggttgaca tcggaatcgg agaaaattgg
     3361 ctggagacaa aatagatatg tttttggttg gtataactgg cggaattgcc agcggaaaaa
     3421 cagaagttgc cagagttttc aaaaaattag gagccacggt catatccggt gacgaattgg
     3481 gccaggaggt agtaaaaaag aacaaattag tcctaagaag attggtccga gtttttggta
     3541 aggtcatttt aaataagaat ggaagtttaa atcgaaagag attagggaga ctggctttct
     3601 tttcaagatc aaatgtcaga aaactgaatt cgatagtcca tccatatctt ctttccagct
     3661 taaaaagaca aataaagaaa ttggaaagaa gcagagatat tgtggcagtg gatgcggctt
     3721 tgattgtgga atggggcata cagaagcaat tcgatttgtt gattttggtt gattcaagaa
     3781 gaaaaaatca gatagagagg ttttgttcca gtaggaaata ttcttcaagg atagggcgtg
     3841 aaataattag gtgccaattg cctaaaaaaa gtaagataat ctatgcagac ctggtgattg
     3901 ccaacaatgg taacttaaag caactcaggg acaaagcgag acaggtttgg tctattatat
     3961 tcgccttatc aagaaagaag aaaaagagga ctatccccaa aaacaggaca tggaaagagg
     4021 gaagtgtttg tccgaattct ggatcttgag acccgaagtc tgtccttcga ccttgccctt
     4081 cggtctgagc tcagggtcga agactcagga cggtgaggac attcggctga gcggttcgac
     4141 cgtcctgagc ggctcaccgt cgaaggactc agtcgaagtc cttgtcgaac cgggtcttga
     4201 gatgagataa ggaaaattga aagaatagga aagtgggaag gtaattcttg tcttcaagaa
     4261 aggaatgttc ttctctatcc tctcccttgg tatgaaccat gacggttcat accttgaagg
     4321 gagaggatag agttgaaaat cccgccaaag gcggagtgag ggtgagaagt atcacggctg
     4381 tgaggctatt ggggtatttc ttttccccct ccctggccgg gcaggcactc tatctctctc
     4441 ccccaagggg ggagagaaac caaaggaaat agccattaag acattctgat gtgttttcaa
     4501 aaaagtgggg atacccctaa agaagaaaag gtttgacact gaaaaataaa ttgcctatat
     4561 tactatgtta aaaatttcac aaggtttagc ttactgagaa tggaggagtt atgccaatat
     4621 tggattcggc aagtggtgag ataagaaaaa gttacagcat agaggagttg aggcaggcag
     4681 ccaaccttat gcgaggatat gacctggtga ctttgtgtgc ggctaattcg ggtcattccg
     4741 gtggcactat gtccatcatg gatattgtag cagcgcttta tttacacatt gcccggcatg
     4801 accctcaagc gcccttctgg gaagatagag acagaatcat attctccact ggacataaag
     4861 ctccgtgttt atatttgggt ttgggcattt caggttattt caatatagag gaggtagtga
     4921 agctacgcaa gttgtacagc ccgtttcagg gacatccgca ttggctaaag ctccccggag
     4981 tggaagcctc gaccggctcc ctgggtcaag ggttaagcat tgcggtggga atcgccttat
     5041 cagctaaatt agataagaaa gactatcgtg cctattgcat taccggagac ggagagcatc
     5101 aggaaggaca gatatgggaa gcggtgatgg aagcaggaca ttataaatta gacaatctgg
     5161 tgtgtgtggt ggataagaat agactgcaga tagacggctg ggtaaatgag gtgatgaata
     5221 tcgacccctt aaatgagaaa tacaaaagtt tcggctggga agttatagag atagatggac
     5281 ataatatgca ggagatcgtt ttcgcttttg agaaagctaa ggggattaaa ggaaagccaa
     5341 ccgtgataat cgctcacacc accaaaggga agggagtgag ctttatggag aacgtggctg
     5401 gctggcacgg aaaggttcca atatgcgacc agctttatgc tgctctgaag gaattgggct
     5461 tggaaggaaa gctcgatgtt gacaagctct tcgccatagc agagaaatat caaaaggaag
     5521 ttaccgagaa gttagaagcc aaacttccta agttcaagcg cgactatttc tggaacaagg
     5581 gtgaaaagat gaaagcggag atggctgcaa cccgattggg ttttggaaaa gccctgcaaa
     5641 aatatggaga tgaccccaga gtggtctgcc tgggtctgga tatctccggc tcaattgcga
     5701 taagcgaatt ctgctccaag aatccggaaa gaaaagagag gttcttgagc atgggcatag
     5761 cggagcagag cgccactgga gtggcggctg gattagccaa agagggtaaa cttccggtat
     5821 tgggaactta tggagtcttc gcctcgggca gaaattggga tcagcttaga accactgtct
     5881 gttacggtaa tttcaacgtg tttgtggcag gagcgcacgg cggggtgtca gttggagcag
     5941 atggcgccac acaccaggca ttagaggaac tatttctgac ttgcgggatt ccgggaatga
     6001 atgtggtggt tccctgtgat atagttgaaa ccgagagggc aaccaagtat ttattgttcg
     6061 agttggctgg tcccaagtat ttgaggttcg cccgcgaagc cacaccgatt gttaccaccg
     6121 aagccactcc ttttgttttc gggaaagcga atgtcattcg ctatcgagaa gagaaggata
     6181 atttcagaga tgcctttgag accaaattag cttcggaata taaatcggaa aaagaggatt
     6241 tgaccataat cggctgtgga cctgaagttc cagaggcgat gagggcagcg tatatcttaa
     6301 aaaaggattt caaaatagat acgagagtgg tgaatatgca taccataaag cctctggaca
     6361 aagaggccat cataagggct gctctggaaa cagggataat cgttaccgcg gaggaacacc
     6421 aggtcggcgg tctgggagac aaagtcagcg cggttgtgtc tcaagccaaa gagctttaca
     6481 ggaaaccgat cattatggga atgataggtg ttaaagatag atttggtgag tcaggtcaac
     6541 cctgggaatt gataaaagag ttcgaagtta gtggggaaca tattgcccag aaagccaaag
     6601 agctttacga tttcaaaaaa ggtaggggca attcatgaat tgcccctaaa gaggaggagc
     6661 gtatgaaatt agccaagagg atgtcccgtc tgggaacgga gactgctttt gaggttttag
     6721 ccagagcaaa gaagttggag gcgcaaggta aaaacatcat tcacctagaa atcggtgagc
     6781 cggatttcga tacccccaaa aacatcacgg atgcggcaat tggggctctc aactccggtt
     6841 ttactcatta tggtccgtca gccgggttgc cggaactaag agaagttata gccaaatacg
     6901 tttccaaaac cagaaatata aaagtggatg gggagaacgt ggtggttacc ccgggtggta
     6961 agccgataat gttcttcgcc attctggctt tggcagatga aggggatgag ataatctatc
     7021 ccaatcccgg ttttcctatt tatgaatcag ttataaattt catcggcgcc aaaccgattc
     7081 cagtaaagtt gagagaagaa aacaacttca gactggatgt gaatgaactt aagaagctgg
     7141 ttacctccaa gaccaaaatg attatcatca actcgccgca aaatcccact gggggtatat
     7201 tgaccaaaga tgacctagga gttattgcac agatagctat caagaatgat ataatggtct
     7261 tagcggatga gatatatagc cgcatgatct atgatggaga gcatcacagc attaccagtt
     7321 tcgatggaat gccggagagg acgataattt tagatggctt ttccaaaacc tacgccatga
     7381 ccggctggag aatgggttat ggcgtgatga ataaagagct gacacctcat atcgccaagc
     7441 ttatgaccaa cagcaattcc tgcaccgcca ctttcactca aaaagcgggc atagaggcac
     7501 tagttggtcc acaggataag gtggaagata tggtaaagga gttcaagaaa cgcagagacg
     7561 tcattgtcaa gggactgaat tcaataccca aggtaagctg taaaacccca caaggtgctt
     7621 tctatgtttt tcccaatatc accaaaaccg gcaagaagag caaagaattg gcagattatc
     7681 tattgaatga gggaggagtg gcggttcttg ccggaacgtc gtttggagaa ttcggggaag
     7741 ggtatttgag attgtcttat gccaattcaa ttaagaacat tgaggaggca ttaaagagga
     7801 taaaagaggg gttagcgaag atgtaatttt attccaattt gttcgtagtc cttttttgca
     7861 ggcgacccat ccgggtcgcc tatgttttta tggttttcgg gcggagataa actccgcccc
     7921 tacgcgtttt acttttttcc ttggatgata aaaatggggt ggaattttag gcgggcgtgg
     7981 aagctggccc ctatgatttg atgtttcgga aataacaatt cccgaagctg aagtttatag
     8041 ggatttacga atccgataca tcaaaacctc tcaccctcac cttgatcctc tccctcaagg
     8101 gagaggatga aaacagtttt aagttttgag tttcaagttg caagtctcaa ggttttcttc
     8161 tttcctcttt tttccttctt ttctttctta tgtttttatc cgttattcgg cattcgtcat
     8221 gcgatattgt tctattattt ttccttgaca ttaagccaaa attcaaatat ataagctcat
     8281 ccttatgcga ataagattcg ataatgagtt ctcccgacct gcctatttgg aagtggattt
     8341 ggatgcctta gcttacaaca tcaaaaatat aagaaagaaa ataggcaaaa aagttgagtt
     8401 tatggcagtg gtcaaggcag atgggtatgg ccatggggct tatcaggttg ccaagaccgt
     8461 tttggaaagg ggggtggact gtttgggagt tgccattttg gaagagggaa taaagttaag
     8521 agaaaaaggt ataaaaggca agatagttaa cctttatccc gaaccaattg gcagggagaa
     8581 gtatgtaata gagtatgaca tcgctcaagt aataacggat tatgcttttg ctttaaacct
     8641 ttccagagaa gccaaaaaac ttgataaaat agcacaagtt tatgtagagg tggatactgg
     8701 tcttggtcgt tatggaatgg ctccagaaaa tgcaattaag ttagtcaaaa aaatatataa
     8761 cttgaaaaat atcagattta aagggattat ctcacagttt tcaactgcgg atgagaaaga
     8821 taagaacttt gccttaaagc agttgtcgat ttttaaaaag accgttcacg atttggagtc
     8881 atctggttat agaatcccca taaaatcaat tgccaatagc ggtgcggtat tagacattcc
     8941 gcaaagttac ttcaatcaag ttagggtggg acatctgatt tatgggcttt atccttctat
     9001 ctacaccagc cagtccatca aggtgaagcc ggtcttgaaa tggagaagca aaatagcgtt
     9061 catcaaagaa gttgaaaagg gaactccatt gggatatggc agaagttatc ataccaaaaa
     9121 gaaatctaaa atcgccacta ttcctttggg atatgcggat ggttacagcc gcttgctttc
     9181 caacaaagca caggttctaa tacataataa aagattgtct gtggtcggtc gggtttgcat
     9241 ggatgctttt atggtggatg ttagttcaat tccggatgtt aaagttggag atgaggtgac
     9301 actaatcggc aggcagggaa aagaagaaat ttccgctcac cagttgggca agtggaccgg
     9361 cactttctcc tatgaggttt taagccgtat tggcaaaaga gttcctatcg tttatttgaa
     9421 aaaaagctga cctcattttt catttggaaa atctcgcctt tggttttata atttaaaaat
     9481 atgtcgaaga taagtttatt ctctacgctt ttggttattc tattgagcgt ttcttcttca
     9541 gcccaggata aagctcctgc aataaatgtg atttatccca ggaatggaca aaccttaact
     9601 tcccctgact ctgctttcat ttttggctca gttacaccca aatcctccct agaaatcgat
     9661 ggagaagata taaaagttta tcccaatggg gcatttttgg ctttcttgcc aattcagccg
     9721 ggcgaattcg tgtttgaact cgaagccaca aacaagttgg ggattgcttt ggatagcgtc
     9781 gaggttctcg ttcccactac ttcaactact attccggaag acagcttagc catcgaaaag
     9841 ggaagtatgt tgccggatgt ggatatgaat ctccaagctg gggatataat tcaggtccgc
     9901 ttcaaaggga cacctggagg taaggcaact ttttcaattg agggattggt ttcaggtttg
     9961 cctatggtag aatccgtatc taataaagaa aaatattggg gagaggaagt ttttggcaaa
    10021 ggggatagtt caagccgtat tcaatccaaa ggggtctaca ctggagctta tcggataaag
    10081 ccagaagata aaattgaaaa tgcgagagtt gtcttttgtt tgactaaaga actgaatgat
    10141 tctctaaagt tcgattcttc tgcaaaggtc gataaaataa acaacaatgt aagtttagtt
    10201 agttgggctc gaggcagaat aaccataagt caagattcga ttcctcaagt agtggagttc
    10261 attgattcga ttcaaattgt gcgagcaggt ccgggattgg gatatttgct ccttttccag
    10321 ccaaaaggga taagggcaat tgccgatggt cagattgggg aatggacaag attaagactt
    10381 gccccagacc aagagggatg ggtaaaaaag agtagtatag aatttcttcc ctcaggcaca
    10441 ccagttcctc gaagtaaagt cagtttgata aaaactctaa gtcaacagga taaagtctcc
    10501 attatagtac ccttaactca gaaactgcct tttaaagtgg agcaggattc atccggactt
    10561 tcactttctg ttttcggagc cactgccaat acagattgga taaaatatga tgcaaaggat
    10621 gacttaataa aacagataac ctggtcggag ccgcagaaat tagtatatca ggttcgtatt
    10681 gatttgaatc agaaacagca gtggggatat gatgcttatt atgataacac agatttgatt
    10741 ttgggtataa ataaaaaacc aaagatagg
//
LOCUS       DASWLN010000112         4575 bp    DNA     linear   ENV 20-FEB-2024
DEFINITION  MAG TPA_asm: Candidatus Zixiibacteriota bacterium isolate
            SMAG_U2949 k141_733100, whole genome shotgun sequence.
ACCESSION   DASWLN010000112 DASWLN010000000
VERSION     DASWLN010000112.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37960594
            Sequence Read Archive: SRR10376091
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Candidatus Zixiibacteriota bacterium (soil metagenome)
  ORGANISM  Candidatus Zixiibacteriota bacterium
            Bacteria.
REFERENCE   1  (bases 1 to 4575)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 4575)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (26-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/12/2024 08:28:26
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 1,440
            CDSs (total)                      :: 1,416
            Genes (coding)                    :: 1,412
            CDSs (with protein)               :: 1,412
            Genes (RNA)                       :: 24
            rRNAs                             :: 1 (16S)
            partial rRNAs                     :: 1 (16S)
            tRNAs                             :: 21
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 4
            CDSs (without protein)            :: 4
            Pseudo Genes (ambiguous residues) :: 0 of 4
            Pseudo Genes (frameshifted)       :: 0 of 4
            Pseudo Genes (incomplete)         :: 3 of 4
            Pseudo Genes (internal stop)      :: 1 of 4
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..4575
                     /organism="Candidatus Zixiibacteriota bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_733100"
                     /isolate="SMAG_U2949"
                     /isolation_source="soil"
                     /db_xref="taxon:2053527"
                     /environmental_sample
                     /country="USA: Oregon"
                     /lat_lon="42.99 N 123.63 W"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            complement(<1..3285)
                     /gene="lptD"
                     /locus_tag="VGB16_03760"
     CDS             complement(<1..3285)
                     /gene="lptD"
                     /locus_tag="VGB16_03760"
                     /inference="COORDINATES: protein motif:HMM:NF040057.3"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0019867 - outer membrane [Evidence IEA];
                     GO_process: GO:0010033 - response to organic substance
                     [Evidence IEA];
                     GO_process: GO:0061024 - membrane organization [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="LPS assembly protein LptD"
                     /protein_id="HEX9916831.1"
                     /translation="MPSKLPLHILRLLLCFMFCSPLWAGEEKPIELLNADFSELRLEK
                     DNTILNLIGDVRFRHEDAILESNRAIWYRNSGQIIFIDSVRMEDGEHILHANRLTYFH
                     KTKKVIADGEVSLQSKKDHSWLTGEHGEYDRQNKQARFTIRPKLVSDYTNLESSVIVT
                     GRVMEYSVDERRGVASGNVEIEKGKMRAFCDTAVYYNQEDKLVLKGHPRAEQEGSKLS
                     GDLMEILLKENKIKEIVVKGDAKATQLEVPDSASTISRESFLSGKEITFFLEKDELRE
                     VEVIGNATSLYYPSAEDTLHRGRNEASGDTINLFLREDKVERVQIKGGAIGVYYNPVK
                     IENDTLTREDTVKYSAESIDYVMNEEMITLEKNSYLQYGQTSLKAGRVKYHTQKQVLT
                     AEGIPVMKDSLGVIVEDPTGEGLPILKDGNEEIAGQGMVYNLKTRRGKIRAGETEFNK
                     GIFTGNQIRKVEDKVLFVNGGIYTTCDQKNPHYHFASPNMKIIAEDKVVVKPLVFYMA
                     DLPVFAIPFYVFPIKPGRHSGFTVFDLGNLESNQRFIRNFGYYWALSDYWDLKTTLDY
                     FEGRGWIFHSRARYAVRYLLNGSLDGSYTRESSWSGFTQSRKSRWDLSFNHYYTLSDV
                     TKLTGSGTFVSDKNYYRDISLNEEQRRNRSLSSKVSLSTRRGDWSITTAVDRSLNLDT
                     DARTDVFPTLSLSKPFLPIFKPQEEETTETENKRWYHSIYYSYSSNLQNYSNRSKVNE
                     DLNWRKHLTFDHHLNLQAPQNAFGWLVLNPSFSYQETWYYVFRTNLSEEKGIPAENLA
                     RRGVYSTSISANTNIYGSFYPHLAGITGLRHVATPSLSFVYQPSFTRHDDFKSYTGRG
                     GGSSAKSESMGFSLNNLFQMKYGEAEKEKKIDLFNLNFSSGYNFVAKEHKLSVLSSSF
                     RSSAIKIVDFDINAVHDFYDENTKELRISSPRLVNFDLSTTFSISGKGIGSQDSEMDT
                     SSSAGGQDQIATTPVSSKGWNLKITQRYGESRRFGTKTINHWAEVSLGLPLTKNWFLN
                     YLNRYDFREKKIISQRFEFYRDMHCWEGKFVWILDGLRRGYYFKLNIKLLPEVKVEKS
                     ERGLKEM"
     gene            complement(3742..>4575)
                     /locus_tag="VGB16_03765"
     CDS             complement(3742..>4575)
                     /locus_tag="VGB16_03765"
                     /inference="COORDINATES: protein motif:HMM:TIGR04183.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="FlgD immunoglobulin-like domain containing
                     protein"
                     /protein_id="HEX9916832.1"
                     /translation="AGAVAILREYNPDATVEQIKDALIRSAVDLGTPGEDNDYGYGLI
                     NIQRALEYLVPPSTPCVYLDSIITEEKSMDSVVCGDTVKIDVNLKNAGTGLGPFRVQL
                     QTSDIGVILFQDEVHFDSLTQEEISSNQDNPFVFYLIPNQEEKIINFKLNIVADNESY
                     SRQIDFNLLTKPGFGLGVNENLNPPTKFSLGQNYPNPFNPSTTIPFDLEKEAYVSLKV
                     YNVKGQLVKTLLDKNLPRGKHEVAWKAEDQDGVKAASGVYFYVLKTNEQYVAKKMFLV
                     K"
BASE COUNT         1223 a         1071 c          862 g         1419 t
ORIGIN      
        1 catttccttc aatccccttt cgcttttctc caccttcact tcaggcaaaa gcttgatatt
       61 cagcttaaag taataacccc ggcgtaaacc gtccagtatc cacacgaact tcccctccca
      121 gcaatgcata tcccgataaa actcaaacct ctgggagatg atcttctttt cccggaaatc
      181 ataacgattg agatagttca aaaaccagtt cttggtcaag ggcaagccca gggagacctc
      241 agcccagtga ttaatagttt tcgttccgaa tctcctgctt tcaccataac gttgcgttat
      301 cttcaaattc catcctttgg atgagaccgg ggtagtggca atctggtctt gtcctccagc
      361 ggagctcgaa gtatccattt cggaatcttg agatccaatc cccttacctg aaatggaaaa
      421 tgttgtgctt aaatcaaagt tgaccagccg cggagaggaa attctcaact ctttagtatt
      481 ttcgtcgtag aaatcgtgca ccgcatttat atcgaaatct actatcttga tcgcgctgga
      541 acgaaaagag cttgaaagaa cagaaagctt gtgctccttt gccacgaaat tgtacccgga
      601 actgaaattc aaattgaaga ggtctatctt tttctctttt tccgcttctc catacttcat
      661 ttgaaagagg ttgttcaaac taaatcccat actctcgctt tttgcactag acccccctcc
      721 cctgccagta tatgatttga aatcgtcatg ccgggtgaag ctgggctgat aaacaaaact
      781 cagactggga gtagctacat gccgcagacc agttattcca gctaaatggg gataaaaact
      841 accgtatatg ttggtattgg ctgatatgga agtgctataa actccacgac gagccagatt
      901 ttcagcaggg attccttttt cctcgcttag attggttcta aacacatagt accaggtttc
      961 ctgataacta aaagaggggt tcaaaaccag ccatccaaaa gcattttgcg gcgcctgcaa
     1021 attcaaatga tggtcgaagg taaggtgctt tctccaattg aggtcctcat tcaccttgct
     1081 gcgattggaa tagttctgta aattggagct gtaggagtag tagatactgt gataccatct
     1141 tttgttttcg gtttcagtcg tttcttcttc ttgcggtttg aatatcggca ggaaaggctt
     1201 ggataaagac aaagttggaa atacatccgt gcgcgcatca gtatctaagt tcaaactgcg
     1261 gtcaactgca gtggttatgc tccagtcccc ccgccgggtg ctcaagctga ctttggaact
     1321 taaactgcgg tttctccttt gctcttcgtt taggcttata tctcgataat agttcttatc
     1381 gctaacgaaa gtgccagagc cagtcaattt agtgacatcc gaaagcgtgt aataatgatt
     1441 aaaagacaga tcccagcggc ttttcctgct ttgagtaaaa cccgaccaag aagattccct
     1501 ggtgtaggag ccatccagac taccatttaa aaggtagcgc accgcatacc tggctcgact
     1561 atggaatatc cagcctctcc cctcaaaata gtccaaagtg gttttcagat cccaataatc
     1621 cgatagtgcc cagtaatatc cgaaattccg aatgaacctc tggtttgatt ccagatttcc
     1681 caaatcgaaa acagtgaatc cagagtgtct tcccggcttg attggaaaga catagaatgg
     1741 aatagcgaag acgggcaaat cagccatata aaagaccaat ggcttaacca caactttatc
     1801 ttctgcgata atcttcatat tcggactagc aaaatgataa tgtggatttt tttggtcgca
     1861 ggtggtatat atgcctccat tcacgaacaa gaccttgtcc tcgacttttc tgatctgatt
     1921 accagtgaat atacccttgt tgaattcagt ctctccagct cttattttcc ctcttctagt
     1981 ttttagatta taaaccatac cctgacccgc tatctcttcg tttccatctt ttaatattgg
     2041 caacccctcc ccggtcgggt cttcgacaat cacccctaag ctatccttca ttactggtat
     2101 cccctcggct gtaagcactt gcttttgggt atgatatttc acccgcccgg ctttaagtga
     2161 agtttgtcca tattgcaggt aactgttttt ctccagagtt atcatctctt cattcataac
     2221 gtaatcaatg ctttccgccg agtattttac ggtatcctct cttgttaggg tatcattttc
     2281 gattttcact ggattataat aaactcctat agcgccaccc tttatttgaa ccctctcgac
     2341 tttatcttcc cttaaaaaga ggttgatggt atctccggag gcttcgtttc tgcctcggtg
     2401 gagagtatct tcggcagatg gatagtatag actggtggcg ttcccgatta cttccacctc
     2461 tcttaattca tccttctcca ggaaaaaagt tatttccttt cctgaaagaa aactctccct
     2521 ggagatagtg gaagctgaat caggaacctc aagctgagtt gccttggcat caccctttac
     2581 cacgatttcc tttattttat tttcttttag cagaatctcc atcaggtctc cgctaagctt
     2641 gcttccttcc tgctctgccc ggggatgacc tttcaagact aatttatcct cttgattgta
     2701 gtaaaccgca gtatcgcaaa aagctctcat cttccctttt tctatttcca cgttcccgct
     2761 ggctaccccc cttctctcat ccacagaata ttccataacc ctgccggtaa ctatcactga
     2821 gctctccaga ttggtgtaat cggaaactag tttgggacgg atggtgaagc gcgcttgctt
     2881 gttttggcgg tcatattctc catgttcgcc tgtcagccag gagtggtctt ttttgctttg
     2941 gagactcact tcaccgtcgg caatgacctt cttggtctta tgaaaataag tcaatctatt
     3001 agcatgaaga atatgctcac cgtcttccat cctgacactg tctatgaaga ttatttgccc
     3061 actattgcga taccaaattg ccctattgct ctccaggatt gcatcctcgt gtctgaatct
     3121 gacatcgcct attagattca aaattgtatt gtctttctct aatctaagct cggagaaatc
     3181 agcattcaac aactcaattg gcttctcctc acccgcccag aggggtgaac aaaacatgaa
     3241 acacaaaagc aagcgtaaga tatgaagagg aagtttcgat ggcatatcaa ataaaaatag
     3301 aaaaatctca accccaggtc aagcaaataa aaaagtagtt tcttaagctt gtagcaaagt
     3361 tattagagtt ttttgttgaa gggtcatctt tgaaaattta tatggtagcc tatctaggat
     3421 gcatcgggaa taggaattcc cgatgttcgt actcctgaat gtgcttcggc atttcctaat
     3481 accgaagcat ccgcagtatc taaaaaagaa aattcgcccc agtgcacaaa aatttattcc
     3541 aacttcttaa gaattaaacc attacagaat atggttgcta caaggttgag aaagcttcaa
     3601 taaaaactct tgattgttat attaagcatc catatcaatc acaatcgggc ttctcagagt
     3661 gaaattctca acagtattat actattagtc tgtttctttg gaggcgggct ttgtagaaac
     3721 tttgcagaat gtttgctcgg attatttcac caagaacatc tttttagcca catattgttc
     3781 atttgttttt aacacataga aatagactcc gcttgctgct ttgacaccat cctggtcctc
     3841 ggctttccaa gctacttcgt gttttcctct gggcagattt ttatccagca aagtcttgac
     3901 cagttgtccc tttacgttgt aaacctttaa gctaacataa gcttcttttt ccagatcgaa
     3961 aggtattgtg gttgatggat taaagggatt gggataattc tgacccaagc tgaacttagt
     4021 tggaggattc agattctcat tcactcctaa accaaatcct ggtttagtta agagattgaa
     4081 gtctatctgg cgactgtaac tttcattatc tgctactatg ttgagtttga aatttatgat
     4141 tttctcttcc tggtttggaa tcaaataaaa cacaaaagga ttatcttggt tagaagatat
     4201 ctcttcctgt gtcagagaat caaaatggac ttcgtcttga aacaaaatca cacctatatc
     4261 ggaagtttgc aattgtactc taaacggccc aagccctgtt cctgcatttt tcaggttaac
     4321 atctatttta actgtatcgc cacaaaccac actatccatt gatttttctt ctgttataat
     4381 gctatccaaa tagacgcaag gggtactggg tggaacgagg tattccaacg ctctctgaat
     4441 atttatcaaa ccataaccat aatcgttgtc ctcacctggt gtgcccaaat caactgcaga
     4501 gcggatgagc gcatctttaa tctgctccac cgtggcatct ggattgtatt cccttaatat
     4561 cgctactgca ccggc
//
LOCUS       DASWLN010000113         4615 bp    DNA     linear   ENV 20-FEB-2024
DEFINITION  MAG TPA_asm: Candidatus Zixiibacteriota bacterium isolate
            SMAG_U2949 k141_987554, whole genome shotgun sequence.
ACCESSION   DASWLN010000113 DASWLN010000000
VERSION     DASWLN010000113.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37960594
            Sequence Read Archive: SRR10376091
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Candidatus Zixiibacteriota bacterium (soil metagenome)
  ORGANISM  Candidatus Zixiibacteriota bacterium
            Bacteria.
REFERENCE   1  (bases 1 to 4615)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 4615)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (26-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/12/2024 08:28:26
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 1,440
            CDSs (total)                      :: 1,416
            Genes (coding)                    :: 1,412
            CDSs (with protein)               :: 1,412
            Genes (RNA)                       :: 24
            rRNAs                             :: 1 (16S)
            partial rRNAs                     :: 1 (16S)
            tRNAs                             :: 21
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 4
            CDSs (without protein)            :: 4
            Pseudo Genes (ambiguous residues) :: 0 of 4
            Pseudo Genes (frameshifted)       :: 0 of 4
            Pseudo Genes (incomplete)         :: 3 of 4
            Pseudo Genes (internal stop)      :: 1 of 4
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..4615
                     /organism="Candidatus Zixiibacteriota bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_987554"
                     /isolate="SMAG_U2949"
                     /isolation_source="soil"
                     /db_xref="taxon:2053527"
                     /environmental_sample
                     /country="USA: Oregon"
                     /lat_lon="42.99 N 123.63 W"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            <1..142
                     /locus_tag="VGB16_03770"
     CDS             <1..142
                     /locus_tag="VGB16_03770"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_008604486.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=2
                     /transl_table=11
                     /product="cupin domain-containing protein"
                     /protein_id="HEX9916833.1"
                     /translation="DGEAKVSISGKPFHLKEGEMIIMPANQPHALKAIKKFKMFLVMI
                     RS"
     gene            186..866
                     /locus_tag="VGB16_03775"
     CDS             186..866
                     /locus_tag="VGB16_03775"
                     /EC_number="2.1.1.-"
                     /inference="COORDINATES: protein motif:HMM:NF024880.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008168 - methyltransferase activity
                     [Evidence IEA];
                     GO_function: GO:1904047 - S-adenosyl-L-methionine binding
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="class I SAM-dependent methyltransferase"
                     /protein_id="HEX9916834.1"
                     /translation="MEYNNILLTRITQLKPQNVLDVGCGCGSFTIKLSFYCTEAVAID
                     LSEDLIERSKKENQKPNITYLCMDGRNLSYPDNSFDLVLERATLHHTREWEKVLNEMI
                     RVSAQHILAEEPLDDPRSEAKKNTMQAQKLFLELQNEVGYSHYPYLPLENLLGYFKKR
                     NIPLETEIIKSDEPLGLDEYFSSFGLFAEKSKRKKYWLDRLDDFKKELNGNKLCESDT
                     LFIAAVKS"
     gene            883..2382
                     /locus_tag="VGB16_03780"
     CDS             883..2382
                     /locus_tag="VGB16_03780"
                     /inference="COORDINATES: protein motif:HMM:NF013739.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016491 - oxidoreductase activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="NAD(P)/FAD-dependent oxidoreductase"
                     /protein_id="HEX9916835.1"
                     /translation="MPEREVIIIGAGLAGLSAGIYAQMNGYRSHIFEHHTQPGGVAAA
                     WKKEDYLIDGGIHFIMGHKPGTAIHKVYQDLGIIPFNRFADMTTYGRFLDEKSGYSVL
                     VTQDLKRLAEDLKRISPVDAQLIDELMVGARSMQGIDASEMGMMNPPELCGPLDGMKE
                     IWKMRRLLRCFAGKYAQSIADYSQDICHPYLRDFINNLFLPDVPVWFVFMLLGLLADG
                     QIGYLEGGCLDFVWSMEKHYKELGGEITCNATVEKILVENDCAVGVGLADHSEYRADA
                     VVSACDGYSTIFDMLEGKYINEKIKKRYDNWKLTRPLVMISYGIEREFADEPTFSIVK
                     LENPLLVKYEIIDHIFIRIFNYSSRFAPQGKTVIQVEFESEWDFWNNLRNKDVFAYDK
                     QKERIAEEVLKRLEVHYPDISLYLEVVDVATPHTFWRYTRNQKGAWGGWLLTKELMNT
                     KIERTLPNLKNLYMAGQWVMPGGVPTSLYSGRHAIQILCHEDKKSFCAG"
     gene            2403..4052
                     /locus_tag="VGB16_03785"
     CDS             2403..4052
                     /locus_tag="VGB16_03785"
                     /EC_number="6.3.5.1"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_013705673.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003952 - NAD+ synthase
                     (glutamine-hydrolyzing) activity [Evidence IEA];
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_process: GO:0009435 - NAD biosynthetic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="NAD+ synthase"
                     /protein_id="HEX9916836.1"
                     /translation="MKITLAQINPTVGDLEGNLKKMISILFSAKKDQSDLVVFPELCL
                     SGYPPKDFLERDWFVQKAQKTLEKLLKVSTDYPTLGILLGTVLPTGKGIGKGLYNSAV
                     LIYQGKILTTQHKSLLPTYDVFDENRYFAPASEIHTVEFKDQTLGISICEDAWNDPEL
                     WPKGKIYTFDPIEELAKKRATLFINISASPFYMGKDQIRYRLIHNHVKKHKIPFLFVN
                     QVGGNDELIFDGRSLGFDKNGNLIAVFPSFKEEIRTIDIDSSGKSKTYVPQDQVESVY
                     QALVLGISDYMKKTNFSKAVIGLSGGIDSALTACLAKEAIGKENILGISMPSPFSSEG
                     SIVDSKKLAENLAIEFKVVPISAIYHSYLETLKKDFKGKLPDVTEENIQARIRGNILM
                     AFSNKLGCLVLSTGNKSEMAVGYCTLYGDMSGGLAVLSDVPKTLVYKLADYINRKSEI
                     IPKEIILKPPSAELKPNQTDQDTLPPYDILDQIIHNYIEEVKSPEEIIKMGFDEKTVK
                     WVVKAVDTSEYKRKQAALGLKVTTKAFGVGRRMPVAAKYEG"
     gene            4054..>4615
                     /locus_tag="VGB16_03790"
     CDS             4054..>4615
                     /locus_tag="VGB16_03790"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEX9916837.1"
                     /translation="MKCHLCLLEKQLVNSHIISEAFYQTVYDEKHKVYLLSTSDKEKT
                     QCKQKGLREKLLCVDCDNSLSPFENYTKELFYSLLISGESYRNGIIFEGIDYKKLKLF
                     QLSILWRAGISQQDSFSEVNLTAEHEEKLRQMILNKNPGEPHEYGCLMVGIKMEQDAS
                     DQIIIMPESGRLDGHRCYRFTFAGCFW"
BASE COUNT         1419 a          866 c         1045 g         1285 t
ORIGIN      
        1 ggatggcgaa gcgaaagttt ctatctccgg taaaccgttt catctcaaag aaggtgaaat
       61 gattataatg cctgccaacc agccacacgc tctaaaggcg atcaagaaat tcaagatgtt
      121 tttggtgatg ataaggtcat aagaagttgg gggggtccgt ctaaagaatt ttctattatc
      181 atatcatgga atataataat atcctcttaa caagaatcac ccaattaaaa ccacaaaatg
      241 ttctggatgt aggttgtggc tgtggaagtt tcaccattaa gctttctttt tactgcactg
      301 aagctgttgc catagattta tctgaggatt taattgaacg tagcaaaaag gagaatcaaa
      361 aaccgaatat cacttatcta tgtatggatg gaagaaatct gtcatatcct gacaacagct
      421 ttgacctggt gctggaaaga gcaactttgc accatacccg ggaatgggaa aaggttttga
      481 atgagatgat aagagtctct gcgcaacata tacttgctga agagcccttg gatgacccaa
      541 gaagcgaagc taagaaaaac actatgcaag cccagaaact gtttctggaa ctgcagaacg
      601 aagttgggta ttcccattat ccatatctac ctttggaaaa tttgcttgga tacttcaaga
      661 aaagaaacat tccgcttgaa actgaaatca tcaaatccga tgaaccttta ggtttagatg
      721 aatatttcag ctcctttggt ctctttgcag agaaaagcaa gagaaagaaa tattggctcg
      781 acagattaga tgacttcaag aaagagttaa atggcaacaa gttatgcgaa agcgatactc
      841 tttttatagc tgcagttaaa tcataaggga attgagcaga aaatgcctga aagggaagtt
      901 atcataatcg gtgcggggtt agcagggctt tctgctggga tctatgctca aatgaatggc
      961 tatcgaagcc acatttttga gcatcacact caacccggcg gtgtagcagc tgcctggaaa
     1021 aaagaagact atctgattga cggtggaatc cacttcatca tgggacacaa gcccggtaca
     1081 gccattcaca aggtttatca ggatttggga atcatccctt tcaatcgctt tgcggatatg
     1141 accacctatg gtcgttttct ggatgagaaa agtggatata gtgttctggt cacacaggat
     1201 ttgaaacgtt tagccgaaga tttgaaacgc atctctcctg tggatgccca gctaattgac
     1261 gaactgatgg ttggtgcccg ctcaatgcag gggattgatg ccagcgaaat gggaatgatg
     1321 aatccgccag agctttgtgg ccctctggat ggtatgaagg agatttggaa aatgcgacgt
     1381 ctcctcagat gtttcgcagg aaaatacgct caatcaattg cggattactc acaagatatt
     1441 tgtcatccct atctgcgaga tttcattaac aacctatttt tgccggatgt gccggtctgg
     1501 tttgtattta tgcttttggg actcttggca gatggccaga ttggatattt agagggtgga
     1561 tgccttgatt ttgtttggtc tatggagaaa cattataaag aattaggtgg ggaaataacc
     1621 tgcaacgcca cagtggagaa aatcttggtg gaaaatgatt gcgctgtggg tgtgggttta
     1681 gctgaccata gtgaatatcg tgctgatgcg gtggtatccg cctgtgatgg gtatagcacc
     1741 atcttcgata tgctggaagg caagtatatc aatgaaaaga taaagaaacg ttatgacaat
     1801 tggaaactga cccgtccttt ggttatgata agttatggaa ttgaaagaga gtttgcggat
     1861 gagcctactt tcagcatcgt caaactcgaa aatccccttc ttgtaaaata tgagataatt
     1921 gaccacatct tcatccgcat attcaactac agctccaggt tcgctcctca aggaaaaaca
     1981 gtgattcagg ttgaatttga gagcgaatgg gatttctgga acaacttacg caataaggat
     2041 gtcttcgctt acgacaaaca gaaggagcgg attgcagagg aggtccttaa aagattggaa
     2101 gtgcattatc ctgatatttc gttgtatctg gaagtagtcg atgtagccac ccctcatacc
     2161 ttctggagat atactcgaaa tcaaaaaggg gcttggggcg gctggctttt gaccaaggag
     2221 ctgatgaata caaaaataga aagaactctg cccaatttga aaaaccttta catggctggc
     2281 cagtgggtta tgcccggcgg tgtgcctaca tccctttatt ccgggaggca tgccatacag
     2341 attctatgtc acgaggataa gaaatcgttt tgtgcaggat aatctaagga gggatttgta
     2401 aaatgaaaat tactttagcc caaatcaatc caactgtggg agacctcgag ggaaacttaa
     2461 agaagatgat aagcatattg ttttcggcta agaaagacca atccgatctg gtggtctttc
     2521 ctgaactctg tctttcagga tatccgccca aagattttct ggaaagggat tggttcgttc
     2581 agaaagccca aaaaactctt gaaaagttac ttaaggtttc aactgattat ccaactttgg
     2641 gcatactttt aggaactgtt cttccaactg gcaagggaat aggcaaaggg ttgtataatt
     2701 cagcagtgtt gatttatcag ggaaaaatcc tgacgaccca gcacaaatcc cttttgccca
     2761 cctatgatgt tttcgatgag aacagatatt tcgcgcctgc atcggaaatc catacagttg
     2821 aatttaaaga ccagactcta ggaatctcca tttgtgagga tgcctggaac gacccggagc
     2881 tctggcctaa aggaaagatt tatacttttg acccgattga ggagctggca aaaaagagag
     2941 ccaccctttt cataaatatc tccgcctctc ctttttatat gggcaaagac cagattcgct
     3001 atcggcttat acataatcac gtcaaaaaac acaaaatccc cttcctcttc gtcaatcagg
     3061 ttggcggcaa tgatgagctt atcttcgatg gcaggagctt gggctttgac aaaaatggaa
     3121 atctgattgc ggttttccct tcttttaaag aagagattag aactattgat atagattctt
     3181 ccggaaaaag caagacctac gttcctcaag accaagttga atcagtttat caggcattgg
     3241 ttttaggaat ttccgattat atgaaaaaga ctaatttctc caaagcagtg attgggcttt
     3301 ccggtggaat cgactccgcc ttgaccgcct gtttggctaa ggaggcgatt ggaaaggaaa
     3361 atatcttagg gatttcaatg ccttctcctt tttcttctga agggagcatt gtggattcaa
     3421 agaagcttgc cgagaatctg gcaattgaat ttaaggttgt gccgatttca gccatctatc
     3481 actcctatct tgaaactttg aaaaaagatt tcaaaggaaa attgccggac gttactgaag
     3541 agaacattca agcccgcatt cggggaaaca tcctgatggc tttttcaaat aagttgggct
     3601 gtctggttct ctccactgga aacaaaagtg agatggcagt gggatactgc accttgtatg
     3661 gtgatatgag tggtggcttg gcagttctct cggatgttcc caagaccttg gtgtataaac
     3721 tggcagatta tataaatcga aaatcggaaa tcatacccaa agagattatc ctaaaaccac
     3781 cttcagctga actcaaaccc aatcaaactg accaggacac ccttccccct tatgatatct
     3841 tagaccagat aattcataat tatattgagg aagtcaaatc tccggaggag ataatcaaaa
     3901 tgggatttga tgaaaagact gtaaaatggg tagttaaagc agttgatact agcgaatata
     3961 aaagaaagca agccgccttg ggcttaaaag taaccaccaa agcttttggc gtgggacgaa
     4021 gaatgcccgt tgctgcaaag tatgaggggt aagatgaagt gtcatctttg tcttcttgaa
     4081 aaacagctag taaactctca tattatatct gaagctttct accaaacagt ttatgatgaa
     4141 aaacacaagg tttatttatt atctacatcg gataaagaaa aaactcaatg taaacaaaaa
     4201 gggcttagag aaaaactcct ttgtgttgat tgtgataatt ccttaagtcc attcgaaaac
     4261 tacactaagg agttattcta tagtcttttg attagcggtg aatcatatcg aaacggaatt
     4321 atcttcgaag gcatcgatta caaaaagctc aagctatttc aactttcaat tctatggcga
     4381 gctggtattt ctcaacaaga ttccttttca gaagtaaatt tgactgcaga gcatgaggaa
     4441 aagctaaggc agatgatatt gaataagaat ccgggcgaac ctcatgaata cggctgtttg
     4501 atggtgggta ttaaaatgga acaagatgcc tcggatcaga taataattat gccggaatct
     4561 ggacgcctcg atggtcatag atgctataga tttacttttg ctggctgttt ttggg
//
LOCUS       DASWLN010000114         8231 bp    DNA     linear   ENV 20-FEB-2024
DEFINITION  MAG TPA_asm: Candidatus Zixiibacteriota bacterium isolate
            SMAG_U2949 k141_734441, whole genome shotgun sequence.
ACCESSION   DASWLN010000114 DASWLN010000000
VERSION     DASWLN010000114.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37960594
            Sequence Read Archive: SRR10376091
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Candidatus Zixiibacteriota bacterium (soil metagenome)
  ORGANISM  Candidatus Zixiibacteriota bacterium
            Bacteria.
REFERENCE   1  (bases 1 to 8231)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 8231)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (26-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/12/2024 08:28:26
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 1,440
            CDSs (total)                      :: 1,416
            Genes (coding)                    :: 1,412
            CDSs (with protein)               :: 1,412
            Genes (RNA)                       :: 24
            rRNAs                             :: 1 (16S)
            partial rRNAs                     :: 1 (16S)
            tRNAs                             :: 21
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 4
            CDSs (without protein)            :: 4
            Pseudo Genes (ambiguous residues) :: 0 of 4
            Pseudo Genes (frameshifted)       :: 0 of 4
            Pseudo Genes (incomplete)         :: 3 of 4
            Pseudo Genes (internal stop)      :: 1 of 4
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..8231
                     /organism="Candidatus Zixiibacteriota bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_734441"
                     /isolate="SMAG_U2949"
                     /isolation_source="soil"
                     /db_xref="taxon:2053527"
                     /environmental_sample
                     /country="USA: Oregon"
                     /lat_lon="42.99 N 123.63 W"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            2..442
                     /locus_tag="VGB16_03795"
     CDS             2..442
                     /locus_tag="VGB16_03795"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_015323917.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="3-isopropylmalate dehydratase"
                     /protein_id="HEX9916838.1"
                     /translation="MYPGKYLPIIDAEEMAKHALEGMDKDFPKKIKKGDIIVTGRNFG
                     CGSSREQAATCLKFAGISCVIAKSFSRIFFRNAINQGLALVQSSDLLENVKEGDEITI
                     DFSSNQIVTKNKSFPFPALDAFVMGILEDGGLIEHTKKILRKSK"
     gene            complement(626..1348)
                     /locus_tag="VGB16_03800"
     CDS             complement(626..1348)
                     /locus_tag="VGB16_03800"
                     /EC_number="3.6.1.54"
                     /inference="COORDINATES: protein motif:HMM:NF012376.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008758 - UDP-2,3-diacylglucosamine
                     hydrolase activity [Evidence IEA];
                     GO_function: GO:0046872 - metal ion binding [Evidence
                     IEA];
                     GO_process: GO:0009245 - lipid A biosynthetic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="UDP-2,3-diacylglucosamine diphosphatase"
                     /protein_id="HEX9916839.1"
                     /translation="MNKNIYFFSDAHLGGNNPQAEQHKETKLLSFLDEVKKEGEKLYI
                     LGDLFEFWFEYNNSMPKDYFKVLKKLKELTDAGIEIDYMVGNHDFWLGDCLENQIGVK
                     IHKHDLSVNHQGKSIFLIHGDGLSKKDIGYRILRKILRNQFCIWLYRLIPPDIGIPLA
                     KWVAGKSRQHTAKRKKHYLEDYRKFAQKKTKQGYDVVIMGHTHHPELVNFDKGIYINL
                     GDWFENYSFAVLGDRGFELKTY"
     gene            1475..2737
                     /gene="murA"
                     /locus_tag="VGB16_03805"
     CDS             1475..2737
                     /gene="murA"
                     /locus_tag="VGB16_03805"
                     /EC_number="2.5.1.7"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_004512354.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008760 - UDP-N-acetylglucosamine
                     1-carboxyvinyltransferase activity [Evidence IEA];
                     GO_process: GO:0009252 - peptidoglycan biosynthetic
                     process [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="UDP-N-acetylglucosamine
                     1-carboxyvinyltransferase"
                     /protein_id="HEX9916840.1"
                     /translation="MDKFVIEGGKRLSGTVDISGSKNAVLPILVASLLVEKGEVTVNN
                     VPDLMDIRTIIKVMEHLGAEVKFYPEAKKVTIKADNLNNHEAPYDLVRKMRASFLVMG
                     PLLARLKKAKVSLPGGCVLGQRPVDLHLAGLSKLGAQITEEHGYVIAEVQELSGNMIY
                     FDRPTHTGTENIIMAATLARGETVIINAACDPEVVDLSNFLNSMGAKIGGAGTPFINI
                     NGVKKLRAVEYSPIYDRLEASTYMMAATITNGYIELKNLNIDHLNPVIHKLKQMGVEF
                     EGNKGKSKIKGPKRLRPISVITYPHPGFPTDMQASIMALTAIADGTSQIRETVFEDRF
                     THVMEYTRLGADIRVSGDRATISGVEKLKGASVMASDIRAGAGLVLAGLAAEGTTEVL
                     RVYHIDRGYEKMEEKLSRLGAEIKRVKT"
     gene            2742..5816
                     /locus_tag="VGB16_03810"
     CDS             2742..5816
                     /locus_tag="VGB16_03810"
                     /inference="COORDINATES: protein motif:HMM:NF013283.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0019867 - outer membrane [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="BamA/TamA family outer membrane protein"
                     /protein_id="HEX9916841.1"
                     /translation="MRKSLKIFLFVLVALLAAFSGASSQERYFGKNKVQYSAFDWYYI
                     QSDNFDIYYYGNGYGLAKYAAKTLENAYQEVRRQLKHTLTKKIPVIIYNCPKDFQQTN
                     VTYEIIEEGIGGFTESFKTRVVVPFDGSYEDFRHVLHHELTHAVMFNKLYGDLVGALL
                     TRQYLFQVPLWFSEGFAEYSSRHGWDLEADMIMRDATINGYLVPLDLAGGYLVYKEGQ
                     SALVYIVKKYSEDKIAEILSQGRRKLSFNSAMEAALGLTEKQFSEEWTKYLKREYWPE
                     IADRQEPQEFAKQLTDHLEDGSYLNEKPEFSPDGDKLAIFSDRRDYTEVYIISSVDGR
                     LIKRLVKAERSGDLESLHSYVSGLAWSPDGYSLAFVSRSGGQDKLFLVRFKNRKIYKK
                     LKFDLDEIVSPSWSPSEDEIAFVGLKDGESDLYVYNLESKQLFRITRDPFEEAEPSWS
                     PDGKFMAFSSDRPTTAQETDTARNLFTYHTPNHNLFVWNKETNKIAALTSDWAKNTSP
                     SWSPDGRRICFVSNRNGIDNLYILDIDSLRSYPITNVLTACFSPSWSKAGDKIAFSSF
                     NKGGWDIFVLKGVNQIEPKEPLTETSYLADLEADTLPKIDTARADTSARVEDKLDELD
                     LSAYVFKSGERVTLSQEKEKKEEVDTSKYIEPEGEYRKNKYKLRFTPDLVSGSVGYDP
                     FYGLSGQSYLMISDIFGNHTILLATDLINTIDQTNFELYYVYLPKRIDYGIAILHTKY
                     YYVDDYDNLFSDRVYGGFLSISRPFNKFSRLELNISHLSVDREYEDPPGPGRSTKSLI
                     TNLSLINDTVLWGITGPINGRRTSLSFNYSPIFSRRSAAFRTWEMDCRNYWHFFKRYS
                     FAFRLASGASYGRDPQRFFLGGTSNWISPKLAAIDVYGLDDLYFGEIVTPLRGYDYYD
                     VVGTRYALLNLEFRYPFVENLTLGFPLRLSFHYITGAMFCDFGAAWNQTKKFKGATTK
                     DGTRLNDIKAGVGFGARVNLGIFVLRFDSAWKTDFNDIAAKPINYFSLGAEF"
     gene            5853..8177
                     /locus_tag="VGB16_03815"
     CDS             5853..8177
                     /locus_tag="VGB16_03815"
                     /inference="COORDINATES: protein motif:HMM:NF015714.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ComEC/Rec2 family competence protein"
                     /protein_id="HEX9916842.1"
                     /translation="MRRPALLAVIFFIFGILSGSFVDLPVFLLFSFLILFIILMLYFF
                     LSGNLRMANLFLILALFLGGFFRYEILSQDFPINHITHFTGLDKYFAIEGRIVDYPDV
                     REDRTYLVVEVEKISSGDKYLRTCGEIMVRIKQPTFRFNYGDEVRFNGYINQPTPKRN
                     PGAFDYRKYLNSKRIFGISYLSQENKIEITRKKTANFYYSSFIYPLRSWILKLFDNTL
                     SALPCALMSGFFLGETRDIPKSIYQMFRDTGTLHLLAVSGSNVGLVVLIFLGFLRLLR
                     VPRLYAAIVTLAVIIVFANLVQNEPSVVRAGIMAGVALVGLLLYKNLDALNIVSFAAL
                     LILVYSPLLLFDVGFQLSFVSVFGIIWFTPKMSRFISKFILQANKNLWRWVIYPSIVS
                     LAVELAVIPLVAYYFNFVPLVTVVANLVIVPLAGLSVFMGCLIIFLGIFSIHLASLAG
                     WAGNLILNSTIYLLNFFAHLPVSRLKVASPSLFFMFNYYLILILVFEIGSSRRLKKIL
                     VFYLVLISSFCVWSSVLGSKPKEIGFVFLDAGRGEVALLGTADGRMVLFNAGGVWGNF
                     NNGERIVIPYLIKYGITGLDALVLTDTSEGNLKSSQSIQREVGVKRTVNCLQDFLEND
                     SLSRIEVGPDNKHKKNSNDKLSTDLVRLKTNEKDGFQFCLVRVKYDKVTFCLLDKRVY
                     GFVDSLPRMDSCTVLVLPEIDVDINKAETLIGLLEPKVVLLTSYDLFEKKDSFLSELR
                     YKFPQVRSYSLRENGAVIFKTDGKNVKTKLTIRE"
BASE COUNT         2367 a         1636 c         1845 g         2383 t
ORIGIN      
        1 tatttatccg gggaaatatc ttcccataat cgatgcggag gagatggcaa agcatgcctt
       61 ggaagggatg gataaggatt ttcccaagaa gataaagaag ggtgacataa tcgtgactgg
      121 aagaaatttc ggatgcggca gttccagaga gcaggcagca acttgcctta agttcgctgg
      181 cataagttgt gttattgcca aatccttttc tcgaatattt ttcagaaacg caataaatca
      241 agggttggca ttggtccaat ccagcgactt attggaaaac gtaaaagagg gagacgagat
      301 aactatagat ttctcatcca atcagatagt aaccaaaaac aaaagctttc catttccggc
      361 tttggatgcg tttgtaatgg ggattttgga ggatgggggg ttgattgagc atacgaagaa
      421 aattttacgg aaaagcaaat aaacataaat atccaaaatt tataatgcag gcgacccaga
      481 cgggtcgcct acgcttgttt tcgtagattg aattacctat tcaaaattgt agagttgcgt
      541 caggagctac tcctgacgga acgaaaaggc gacattaatt aatgtcgcct gtagtttttt
      601 aatgataatt accaatcaat aaaagtcaat atgtcttcag ctcaaatccc ctatcaccca
      661 gcaccgcgaa actataattc tcaaaccaat cccccagatt tatataaatg cctttatcaa
      721 aattcaccag ttccggatgg tgagtgtgtc ccattatcac cacatcatat ccctgtttag
      781 ttttcttttg ggcgaattta cgataatctt ccagatagtg cttttttctt ttggcagtat
      841 gctgtctgct ctttccggca acccatttgg ctaaaggaat gccaatatcc ggaggaatca
      901 ggcgataaag ccagatacaa aattgattgc gcaggatttt tctcaaaatc ctatacccga
      961 tatctttttt agacaagcca tcaccatgaa tcaagaatat cgatttgcct tggtggttta
     1021 cgctcaaatc gtgcttatga atttttacac caatctggtt ttccaaacaa tcccctaacc
     1081 aaaaatcgtg gtttcccacc atgtaatcta tctctatgcc agcgtctgtt agctccttaa
     1141 gcttcttcag caccttgaag tagtccttag gcatgctgtt gttatattca aaccagaatt
     1201 caaacaggtc gcccaagatg tagagttttt caccctcctt tttcacttca tccaaaaaag
     1261 atagaagctt tgtttcctta tgttgctcag cttggggatt attcccaccc aaatgggcat
     1321 ccgagaaaaa atagatattc ttgttcatgt ctctcggtaa aattacccca acttaactaa
     1381 aagtcaagcc caaataagcc agcttttaaa agattgcttg actttgtttc acgaaactta
     1441 taattttaat gacccgaaaa ttaaggagcg cagtatggat aagttcgtca ttgaaggtgg
     1501 gaaaaggtta tccggcacgg ttgatattag cggctccaaa aacgctgtcc tgccaatatt
     1561 ggttgcctct ctcttggtgg agaaggggga ggttacggtt aataatgttc ctgatttgat
     1621 ggacatcaga accataatca aggtgatgga gcatctggga gctgaagtga aattttatcc
     1681 tgaagctaag aaggtaacca tcaaagcgga caatttaaac aatcacgaag cgccttacga
     1741 tttagtgcga aaaatgagag cctcttttct ggtgatgggt ccgcttctgg ctagattgaa
     1801 gaaagcaaaa gtctccttac cgggcggttg tgttttaggt cagaggccgg ttgatctgca
     1861 tcttgcagga ctttctaaac tgggtgctca aataacggaa gagcacggtt acgtcatagc
     1921 tgaagtccag gaactaagtg gcaatatgat atattttgac cgtcccactc ataccggcac
     1981 agaaaacata ataatggcag caacgttagc tcgaggggaa actgtaataa tcaatgcggc
     2041 ttgtgaccca gaagtagttg acttgtctaa ctttctcaat tctatgggag ccaaaatagg
     2101 gggtgctggc acaccattca taaatatcaa cggagtaaaa aaattgagag cagtagaata
     2161 ttctccaata tatgaccgct tagaagcgtc tacatatatg atggcagcca ccatcaccaa
     2221 tgggtatatc gaactcaaga atctaaatat tgaccatcta aatccggtga ttcacaaact
     2281 aaaacagatg ggggttgaat ttgaaggtaa caagggaaag tcgaaaataa aaggtcccaa
     2341 gagattgagg ccgatatctg ttatcactta tcctcatcct ggttttccga ctgatatgca
     2401 agcatcgatt atggcactaa ccgcaattgc agatggtact agccagatac gggaaacggt
     2461 ttttgaagac aggttcacgc atgtgatgga atatacccgc ctgggcgctg atataagagt
     2521 ctccggggat agagctacaa ttagcggagt tgaaaagctc aagggagcct cagttatggc
     2581 aagcgatatt cgggcagggg caggtttagt tttggcaggt ttggcagctg aaggaacaac
     2641 cgaggttcta cgggtgtatc atattgatag aggatacgaa aaaatggaag aaaaactctc
     2701 tcgcttagga gcagagataa agagggtcaa gacctaaact tatgagaaag agtttaaaga
     2761 tatttctatt tgtgcttgtg gcattgcttg cagcattttc tggtgcttct tcacaggaaa
     2821 gatattttgg caagaataag gtacagtatt ctgccttcga ttggtattac atccaatctg
     2881 ataatttcga catatactac tacggaaatg gatatggttt agcgaaatat gccgccaaga
     2941 ccttggagaa cgcttaccaa gaagtaagaa gacaactaaa acacaccctc accaaaaaaa
     3001 tcccggtaat aatctacaat tgccccaaag atttccagca gaccaacgtc acctatgaaa
     3061 tcatagagga aggaataggc gggtttactg aaagcttcaa gaccagagta gtggtcccct
     3121 ttgatggctc atatgaagat ttcaggcatg tgcttcatca tgagctgacc cacgcagtta
     3181 tgttcaacaa gctttacggt gacttggtgg gtgcgctttt gacaagacaa tatctctttc
     3241 aagtgccttt gtggttttct gagggtttcg ctgaatactc atctcggcat ggctgggacc
     3301 tggaagcgga tatgataatg agagatgcaa ccatcaatgg ctatctcgtc ccgttggact
     3361 tggctggtgg ctacttggtt tataaagaag gacaatctgc cttagtgtac attgtgaaaa
     3421 aatatagtga agacaagata gctgagattc tctcccaagg cagaaggaaa ctgagcttca
     3481 atagtgccat ggaagctgct ctggggttga ccgaaaagca attcagcgaa gaatggacga
     3541 aatacctcaa gcgagaatat tggcctgaga tagccgaccg ccaggaaccc caggagttcg
     3601 ccaagcaatt gaccgatcat ttggaagatg gttcgtactt gaacgagaag ccggaattct
     3661 cgccggatgg agacaagctt gccatattct ctgaccgcag ggactacact gaggtataca
     3721 ttatttcttc agtggatgga agactcataa agagattagt caaggcggaa agatcagggg
     3781 atttggaatc tctgcactct tacgtgagtg gcttggcttg gtcaccagat ggatacagtt
     3841 tagcttttgt ctccagaagc ggtggacagg ataagctctt tttggtgaga ttcaaaaaca
     3901 gaaaaatcta taagaagctg aaatttgatt tagatgagat agtgtctccg agctggtctc
     3961 cgagtgaaga tgagatagct tttgtgggcc tcaaagacgg ggagagtgac ctttatgtct
     4021 ataatctgga atccaaacaa ctcttcagga taacacgtga cccctttgag gaagcagagc
     4081 cgagctggtc accggatgga aagtttatgg ccttttcttc tgatagacct acgactgctc
     4141 aggagactga tacagcaaga aatttattta cctatcatac tcctaaccat aacctttttg
     4201 tctggaacaa agagaccaat aagattgctg ccttaacttc tgattgggca aaaaacacct
     4261 cacccagctg gtctccggat ggaagaagaa tctgttttgt ttccaatcgc aatggaatag
     4321 ataaccttta tatactggat atagacagct tgcgttccta tcccatcacc aatgttttaa
     4381 ctgcttgctt ctcgccctcc tggtcaaagg ctggagataa gatcgctttc tcctctttta
     4441 ataagggcgg ctgggatatc tttgtgctca aaggcgtaaa ccagattgag ccaaaagagc
     4501 cattgaccga aacctcatat ctcgctgact tggaagcgga cactctccca aagattgata
     4561 ctgccagagc ggatacatcg gcaagagttg aggataagct ggatgagctc gacttgagcg
     4621 catatgtttt caagtcagga gagagggtaa ctttaagcca ggagaaggaa aagaaagaag
     4681 aagttgatac ttcaaaatat attgagcctg aaggggaata tcgtaagaat aaatacaagc
     4741 taagatttac cccggacttg gtttcgggct ccgtgggtta cgaccctttc tacggactta
     4801 gcggtcagtc ctacttaatg ataagcgata tctttggaaa tcacaccata ctcctggcca
     4861 cggacttgat aaataccatt gaccagacta acttcgagct ttattatgtt taccttccta
     4921 agaggattga ctacgggata gccatattgc acactaaata ttattatgtc gatgattacg
     4981 acaatctatt ctcagacaga gtgtatggtg gtttcttgag catatcccga ccttttaata
     5041 agttctcacg gttggaactg aatatcagcc atctatctgt ggatagagaa tatgaggacc
     5101 caccgggtcc cgggagaagc actaaaagtc ttattacgaa cctatcttta attaacgata
     5161 ctgtgttatg gggaatcacc ggtcccatta atggcagacg cacctccctt agttttaact
     5221 attcaccaat cttcagccgg agaagtgccg ccttccgaac ctgggagatg gattgtcgaa
     5281 attattggca tttcttcaaa agatacagtt ttgcctttcg tttagcttcc ggggcaagtt
     5341 acggcagaga ccctcaaaga tttttcttag gaggaacatc caactggata agcccaaaac
     5401 tggcagctat agatgtttat gggttagatg atctctattt cggagaaatc gttactcctt
     5461 tgagaggtta tgactactat gacgtggtgg gcaccagata cgcccttttg aatttagagt
     5521 tcagataccc gtttgttgag aacttgacgc tcggttttcc cttgagatta tctttccatt
     5581 acatcaccgg agctatgttc tgcgatttcg gtgcggcctg gaaccagacc aagaaattca
     5641 agggtgcaac caccaaggat ggcaccagat taaacgatat aaaagcagga gttggcttcg
     5701 gtgccagagt aaacttaggt atcttcgtgt tgagatttga ttctgcctgg aaaacagatt
     5761 tcaatgatat tgcagccaaa ccgataaatt atttctcctt aggggcggaa ttctaataag
     5821 ttttcccgcc agaatcctag tatatagaat agatgagaag acctgcttta ttagcagtca
     5881 ttttcttcat atttggaatt ctctcaggca gcttcgttga cctgccggtt ttcctgcttt
     5941 tctccttttt gatccttttc ataatcttaa tgctctattt ctttctaagc ggcaatttaa
     6001 gaatggctaa tctgttcttg attttggctc tattcttggg aggatttttc agatacgaaa
     6061 tcttaagcca ggattttccc atcaatcaca ttactcattt cactggattg gacaagtatt
     6121 ttgcgattga gggaaggata gttgattatc ctgacgttag agaagaccga acctatcttg
     6181 tggtagaggt tgagaagatt tcaagcgggg acaaatattt aagaacttgt ggtgagatta
     6241 tggtgagaat caagcaaccc accttcaggt tcaattacgg ggatgaggtc cgctttaatg
     6301 gctacatcaa tcagccaacc cctaaaagga atccaggagc atttgactac aggaagtatt
     6361 taaatagcaa aagaatcttt ggaataagtt atctttccca agaaaataag atagaaatca
     6421 caagaaagaa aaccgccaac ttttactact ccagcttcat ctatccacta agaagttgga
     6481 tactcaagct ctttgataat accctctccg ccttaccttg tgctttgatg tctgggtttt
     6541 tcttaggaga aactcgcgat atccccaaaa gcatttacca aatgtttcgt gatactggta
     6601 ctctccatct tctggcggtg agtggctcca acgttggttt ggtggtactt attttcttgg
     6661 gattcctcag attgttgaga gtccccagac tttatgccgc catagtcacc ttggcagtaa
     6721 ttattgtctt cgctaacttg gtccagaacg agccttcagt ggtcagggcc ggaataatgg
     6781 cgggagtggc tctggttggt cttcttttgt acaaaaacct ggatgcgctc aatatagtct
     6841 catttgctgc acttctaatt ctggtttact cgccacttct tcttttcgat gttggctttc
     6901 agctctcatt tgtttcagtt tttggaatta tatggtttac tcccaaaatg agtagattca
     6961 tctcgaagtt tattctccaa gccaataaga atttatggag atgggtaatt tatccttcga
     7021 tcgtctccct ggcggtggag ttggcggtga tacccctcgt ggcatactat ttcaattttg
     7081 tgccactagt aaccgtagtc gcaaatcttg tcattgttcc cctggcaggc ctctctgttt
     7141 ttatgggatg cctcatcata ttcttgggga tattcagtat ccacttagcc agcttggctg
     7201 gttgggcagg aaatctaatt ctaaattcaa ctatatatct tttgaatttc tttgctcacc
     7261 tgccagtctc gcggctcaaa gttgcctctc cttccctctt tttcatgttt aattactatc
     7321 ttattttaat tcttgtcttt gagatcggaa gctctcgccg gctgaaaaag attctggtat
     7381 tctacctggt tttaatatcc tctttttgcg tttggtcgtc tgtgctgggc tcaaaaccca
     7441 aagagatcgg atttgtcttt ttggacgcag gtaggggaga ggtggctttg cttgggactg
     7501 ctgacgggag aatggtcctt ttcaacgctg gtggggtctg ggggaatttt aacaacggag
     7561 aaaggatcgt tattccttat ttgattaagt acggaataac tggtttggat gcgttggttc
     7621 ttacagatac aagcgagggt aacttgaaat caagtcagtc tattcagcgt gaagttggag
     7681 tcaaaagaac agtaaattgt cttcaggatt ttttagagaa tgacagcctc tcaagaattg
     7741 aggttggacc tgacaacaag cacaaaaaga actcaaatga taagctctca accgacttgg
     7801 tgcggctcaa aacgaatgaa aaggatggct tccagttttg tctggttaga gtcaaatacg
     7861 acaaagtcac tttctgtttg ttggataaga gggtttacgg atttgtagat agccttccaa
     7921 ggatggacag ttgtactgtc ttagttctgc cggaaatcga tgtagatata aataaggcag
     7981 agacattaat tgggttgttg gagccaaagg ttgtcctttt gaccagttat gatctttttg
     8041 aaaagaaaga ttctttccta agtgaattga gatacaaatt tccccaagta agatcttatt
     8101 ctcttcgaga aaatggggca gtgatattta agacagatgg caagaatgtc aaaacgaagt
     8161 tgaccataag agaataatag ttgccctaaa gatgattttc atttatattt gagaaagatg
     8221 actcagggag g
//
LOCUS       DASWLN010000115        21480 bp    DNA     linear   ENV 20-FEB-2024
DEFINITION  MAG TPA_asm: Candidatus Zixiibacteriota bacterium isolate
            SMAG_U2949 k141_482131, whole genome shotgun sequence.
ACCESSION   DASWLN010000115 DASWLN010000000
VERSION     DASWLN010000115.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37960594
            Sequence Read Archive: SRR10376091
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Candidatus Zixiibacteriota bacterium (soil metagenome)
  ORGANISM  Candidatus Zixiibacteriota bacterium
            Bacteria.
REFERENCE   1  (bases 1 to 21480)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 21480)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (26-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/12/2024 08:28:26
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 1,440
            CDSs (total)                      :: 1,416
            Genes (coding)                    :: 1,412
            CDSs (with protein)               :: 1,412
            Genes (RNA)                       :: 24
            rRNAs                             :: 1 (16S)
            partial rRNAs                     :: 1 (16S)
            tRNAs                             :: 21
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 4
            CDSs (without protein)            :: 4
            Pseudo Genes (ambiguous residues) :: 0 of 4
            Pseudo Genes (frameshifted)       :: 0 of 4
            Pseudo Genes (incomplete)         :: 3 of 4
            Pseudo Genes (internal stop)      :: 1 of 4
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..21480
                     /organism="Candidatus Zixiibacteriota bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_482131"
                     /isolate="SMAG_U2949"
                     /isolation_source="soil"
                     /db_xref="taxon:2053527"
                     /environmental_sample
                     /country="USA: Oregon"
                     /lat_lon="42.99 N 123.63 W"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            400..690
                     /locus_tag="VGB16_03820"
     CDS             400..690
                     /locus_tag="VGB16_03820"
                     /inference="COORDINATES: protein motif:HMM:NF017103.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF721 domain-containing protein"
                     /protein_id="HEX9916843.1"
                     /translation="MSKPQPLGLVLSKILKTSGMDKGIKQRQAILIWDKVVGQKMASQ
                     SKAIKVEDSKIFVKVKNPVWRSEILLMKSQIAEALNKNLGQRVIKDVIVLGD"
     gene            719..2656
                     /gene="gyrB"
                     /locus_tag="VGB16_03825"
     CDS             719..2656
                     /gene="gyrB"
                     /locus_tag="VGB16_03825"
                     /EC_number="5.6.2.2"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_018086823.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0009330 - DNA topoisomerase type II
                     (double strand cut, ATP-hydrolyzing) complex [Evidence
                     IEA];
                     GO_function: GO:0003918 - DNA topoisomerase type II
                     (double strand cut, ATP-hydrolyzing) activity [Evidence
                     IEA];
                     GO_process: GO:0006265 - DNA topological change [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="DNA topoisomerase (ATP-hydrolyzing) subunit B"
                     /protein_id="HEX9916844.1"
                     /translation="MEKEEDRKDTSLKGHHYDATTIQVLKGLEAVRRRPAMYIGDTST
                     RGLHHLVYEVVDNSVDEALAGFCDQIKVSINKDNSVTVEDNGRGIPVDMHPTQKKPAL
                     EVVMTMLHAGGKFDHYTYKVAGGLHGVGVSVVNALSEWCEVEVSRNGQVYFQRYEKGN
                     TATPMKVIGKKKTCGTKTTFKPDSEFFKDMNFSYEIISAHLKELAFLNKGLKIQIEDK
                     RSGKSNSFEYSGGIVSFVEHLNENKNLIHSKPIYFQHQKEGVELEVALQYNDGYVENI
                     LSYVNNIKTIEGGTHLIGFKTAFTRSINSYAQKNNLLKNSGFSLEGEDVREGLTAVIS
                     LKIPDPQFEGQTKTKLGNSEVKGIVEQITGEKLSEYLVEFPSVAKKITEKSISAARSR
                     EAARKARDLTRRKSALESGRLPGKLADCSLTDPEHCEIYIVEGDSAGGSAKQGRDRRF
                     QAILPLRGKILNVEKAPLDKVLSNEEIRTLITAIGTGIGGDEFDISKARYNKIIIMTD
                     ADVDGSHIRTLILTFFYRYMKDLIEQGRIYIAQPPLYRIRKSKEQHYVYSDDEKDRTI
                     ARLGGGDGVEIQRYKGLGEMNPEQLWQTTMDPERRTLLQVSLEDAAEAERIFSILMGD
                     QVEPRRQFIEENAKYVRNLDV"
     gene            3102..3680
                     /locus_tag="VGB16_03830"
     CDS             3102..3680
                     /locus_tag="VGB16_03830"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEX9916845.1"
                     /translation="MSKIIIWLKTYLYLILPAIVILAIVIVSIVFCLSASKIIQASVA
                     GGTFLLAIAAFGSIAESRKQFRISKSPIFDVMIGGQIINNSNEIKLPLSFENIGYGTA
                     FRVSIELKQGALAWNLEFPLNEFQEIGIGYAWTVGKKKTMTFSPREIAPAQIEKPLEL
                     EIKCDNIFRRRVVQKFSGNMKHGFRIENIEFA"
     gene            3798..6284
                     /gene="gyrA"
                     /locus_tag="VGB16_03835"
     CDS             3798..6284
                     /gene="gyrA"
                     /locus_tag="VGB16_03835"
                     /EC_number="5.6.2.2"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_014558880.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003677 - DNA binding [Evidence IEA];
                     GO_function: GO:0003916 - DNA topoisomerase activity
                     [Evidence IEA];
                     GO_function: GO:0003918 - DNA topoisomerase type II
                     (double strand cut, ATP-hydrolyzing) activity [Evidence
                     IEA];
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_process: GO:0006259 - DNA metabolic process [Evidence
                     IEA];
                     GO_process: GO:0006265 - DNA topological change [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="DNA gyrase subunit A"
                     /protein_id="HEX9916846.1"
                     /translation="MPFESEKIVPVFLEEEMKSSYIDYAMSVITARALPDIRDGLKPS
                     NRRILVAMHDLNLAPNKAHRKCAKIAGDTSGNYHPHGEQVVYPTLVRMAQDFNMRYLL
                     VDGQGNFGSIDGDSPAAMRYTEARLAPLAMEMLADIEKETVNFVPNYDVTREEPTVLP
                     SKFPNLICNGSSGIAVGMATNIPPHNLGEIVDAMVALISDSDVEDEKLLEYVTGPDFP
                     TGGIIYGTAGIGEAYKTGRGKIILRAKANIERQKSGKESIVITEIPYQINKSNLLERI
                     AELVQNKEIEGISDLRDESDRDGMRVVIELKREAPAEIVLNQLFKHTQMQTTFGIIML
                     ALAKGEPKVLNLRQMLDGFIQHRHEVVTRRAKFDLKKAEERAHILEGLKIALDNIDAI
                     IKLIRASKDVDTARQGLMKSFKLSQIQAQAILDMRLQRLTGLERKKIEEEYLELIKEI
                     ARLKGILESKAKRMAIIKDELLELKKKYADERRTEIKEAEEEFTIEDLIAEEDMVITI
                     THSGYIKRLSVSAYRRQGRGGKGVIGIETKETDFVEHLFIASTHDYILFFTQKGRCYW
                     LKVHEIPVGGKMAKGRPIVNMLELSEEEKVAAFVPVKEFDDKHYVIMATKNGTIKKTV
                     LSAYSNPRKGGINAIGIDEDDTLIEAKITDGNCDIVIATKDGQAIRFPENKVRQMGRT
                     AYGVRGISLEKGDEVIGMVVIKRDTTLLSVTENGYGKRSQISEYRVTNRGGKGVINIK
                     TTERNGKVVSIKEVQDEDELMLITQKGIVIRQPIKTIKTIGRNTQGVRLINVSQGDRV
                     VDVARVVKSEGNGNGEGNGGEGTADAVNGQ"
     gene            complement(6544..7596)
                     /locus_tag="VGB16_03840"
     CDS             complement(6544..7596)
                     /locus_tag="VGB16_03840"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEX9916847.1"
                     /translation="MKYKVLLLIVLIFLVSCSERVHSPVEQQEWKCLGLGGRWIHKLR
                     LKGNVLYAGTDDGVYRKDITSPDTIWTPIGLQGKHAVALLVVHPDTILASVVITEVGS
                     DTLSLFRTTDGGTDWQAFQNGFGGGGSHQVMALAKFPGQSDTLFATGACVVAKSTDGG
                     SSWRRVWGGWHYGGVATHFITVDSASPNTVWAGGESGFFAAYLVKSTDRGENWQTTYP
                     DVGGDNACLGIAIDPSNSDVVYVSMEGKVIKTIDGGTIWNIVLEEVSAYLFAIAINPY
                     YPSVVYVTGGVPTPQPLILYKTRDAGCSWFPISQDAGLTLDVRDFLLLSNGKVDKLYL
                     GTIQDGVYQYTALTQK"
     gene            complement(8146..9330)
                     /locus_tag="VGB16_03845"
     CDS             complement(8146..9330)
                     /locus_tag="VGB16_03845"
                     /inference="COORDINATES: protein motif:HMM:NF024685.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="SIR2 family protein"
                     /protein_id="HEX9916848.1"
                     /translation="MIPPLSSKLVFLFGSGVSIPAGMPSTKDITDQVLSANNIMRHTD
                     GNYYFGQPLYAHVGIPDEYVPRVKNFIEILMPEIGRYYNYNPERKTNYEDIYYVVSQI
                     HDSELEELDNPVAQPFIDKIRANVESLLQGKEGETRKNWRLHELADETTHYIADIAWN
                     MLSKKPNRLDYLNLVKDISRDNNLSHIDIFTLNHDTVLEQCLCQNNIPFNDGFGEPIN
                     GVRYWDNKLFETSACKIHLFKLHGSVDWFRLRPAGGGWYEESIGIPLNSITYSHIDKV
                     DGRPLLLIGTFNKMLEYLRGIYTSLFTYFYKSLHDAERLVIIGYRFGDKGINTQIIQW
                     LYSSQKNKITVIDPNPNLKNAARGAISNKWDNWINDDKLTFLQTKLEEISWKDIKNNL
                     SI"
     gene            complement(9332..9907)
                     /gene="pyrE"
                     /locus_tag="VGB16_03850"
     CDS             complement(9332..9907)
                     /gene="pyrE"
                     /locus_tag="VGB16_03850"
                     /EC_number="2.4.2.10"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_004401218.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004588 - orotate
                     phosphoribosyltransferase activity [Evidence IEA];
                     GO_process: GO:0009220 - pyrimidine ribonucleotide
                     biosynthetic process [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="orotate phosphoribosyltransferase"
                     /protein_id="HEX9916849.1"
                     /translation="MSEQNEVLEIFTKSGAILKGHFLLSSGLHSDEYFEKFQVLQYPH
                     YVEMLCKKLADKFEDKKIGVVVGPTVGGVIVAYEVAKNLGVKAIFAEREEGERVFKRG
                     FSLNEGQRVLIVDDILTTGKSIFEVISLVKKYKAYIVGIGVFLDRSGGTVKMDYDLKP
                     LVTVSAKTYKPEECPLCKSGEPIYKPGSRKG"
     gene            complement(10552..11280)
                     /locus_tag="VGB16_03855"
     CDS             complement(10552..11280)
                     /locus_tag="VGB16_03855"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEX9916850.1"
                     /translation="MQKVLEEIKAVQGVIGVLIWDKKTLRSSELLPASFATSAIKSTC
                     SKIIKLGQDMKSQGEVKLFYANGSAIILSRPSALILILARPDLNFSLLNLVLGASLLR
                     LEKILGAEEVGKLNFPPLEQKKVNLLIEGMNLLSRFVSDKLGPYRTTQNLRQAKEKLV
                     TSYLFMANLFVDNNANISVIKGKEGTWSGEVIMAFAKWAAHLEKLSFKKERVLDLKKI
                     TSPHQESLEEMGFYFVFQNIRENI"
     gene            complement(11378..11983)
                     /locus_tag="VGB16_03860"
     CDS             complement(11378..11983)
                     /locus_tag="VGB16_03860"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEX9916851.1"
                     /translation="MSSIQPAGISSYLSWKEKKLYLQTEFALRPQPRITTTVSCEGEV
                     IHKVEEAWEGGLRREEDKKKLESLIRRQHKQVRSVVRNKAEEILADHMPKPLPKFWEQ
                     LSKMEGIENTFAFDQEGKVLYQERESDVSKKITENVISAIQLANFLSQLSKFGNFNKS
                     IIQTDDFKVILFQRRDNYFAVHLNKEKEIEAVIHKIENALK"
     gene            complement(12162..14405)
                     /locus_tag="VGB16_03865"
     CDS             complement(12162..14405)
                     /locus_tag="VGB16_03865"
                     /EC_number="1.17.4.1"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_013276855.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="vitamin B12-dependent ribonucleotide reductase"
                     /protein_id="HEX9916852.1"
                     /translation="MEKKIQNELKLTENALKVLEKRYLAKDDEGKIIETPEEMFWRVA
                     RFIAGPDEKYGASPEETEEIARNFYEIMTNLEFMPNSPTLMNAGRPLGQLSACFVLPI
                     EDSMESIFTAVKDTALIHKSGGGTGFSFSRLRPKNSVVASTSGVASGPISFMKVINSA
                     TEAVKQGGTRRGANMGVLRIDHPDIPEFISSKDDLSELNNFNISVAVTDDFMRAVDRD
                     EYYPLIDPRTKKEYFRDTKPVRLRAKEVFNQIVEHAWRSGEPGIVFIDRINRDNPTPN
                     VGEIEATNPCGEQPLLPYESCNLGSVNLSLMLKAVVDSEHANLFRYEIDWDKLAKTVR
                     AAVHFLDNVIDANKFPLSQIEKNTLANRKIGLGVMGWADMLIQLRIPYDSSQAVNLAE
                     EVMFFVQDEAHKASSDLSRVRGDFPNWGKSIYRQEGMPIRNAAVTTIAPTGTIGIIAG
                     CSSGIEPLFAVSYIRTVMDNTKLVEANPYFKQEAQTKGFYSPELMEKIANTNSLKGFS
                     EIPEETKNVFVTSHQISPEWHIRMQAAFQKHTDNAVSKTINLPNQAKLEDVELAYKLA
                     FELGCKGVTIYRDGSREDQVLSTKKGKESKEKVKVQERPDCLTGITDKIKTGYGNLYV
                     TVNILEGKPFEVFAQIGKSGYSTMADTEAVCRLISLALRSGVSMHRIIEQLIGIGGAS
                     PVFQNGGLIMSIPDAIAKVLNKHFGSGKNHISDPDLGVDLCPDCGSRLEHKEGCNVCR
                     HCGYSKC"
     gene            complement(15066..15725)
                     /gene="thyX"
                     /locus_tag="VGB16_03870"
     CDS             complement(15066..15725)
                     /gene="thyX"
                     /locus_tag="VGB16_03870"
                     /EC_number="2.1.1.148"
                     /inference="COORDINATES: protein motif:HMM:TIGR02170.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0050797 - thymidylate synthase (FAD)
                     activity [Evidence IEA];
                     GO_process: GO:0009265 - 2'-deoxyribonucleotide
                     biosynthetic process [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="FAD-dependent thymidylate synthase"
                     /protein_id="HEX9916853.1"
                     /translation="MSPKKTKKNMKVELISITPKAEDLIERACRTCYLSFHRYNPPSS
                     TRELIKKVIRKGHHSVLEHASATFRIKGGSRVFTHELVRHRLLSPSQESQRYVEYGKT
                     KEFEFVIPPTIEKTKFKKEFTNFAKKSYELYSQMVKADIPKEDARYILPNATTSEIVI
                     TANFRELRHIFQVRCQERAHWEIREICLEMLKIMKKKAPIVFWDFEVDEKKRVAVRKE
                     L"
     gene            complement(15925..16443)
                     /locus_tag="VGB16_03875"
     CDS             complement(15925..16443)
                     /locus_tag="VGB16_03875"
                     /inference="COORDINATES: protein motif:HMM:NF024231.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ECF transporter S component"
                     /protein_id="HEX9916854.1"
                     /translation="MKFLNREISLTGLFIALGVALPIAFHFINPLAGRTLLPMHLPVL
                     LAGILLGPISGIIVGLVCPTASFLLTGMPPAYAVPLMTLELVLYGFASGILMRTLRIP
                     LIVGLLLAMIVGRLGFAFALLILGRFINLPYGIKEFILVAIPTGLPGIILQIILVPIL
                     VKGIQLASRTEM"
     gene            complement(16440..17036)
                     /locus_tag="VGB16_03880"
     CDS             complement(16440..17036)
                     /locus_tag="VGB16_03880"
                     /inference="COORDINATES: protein motif:HMM:NF014414.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="methyltransferase domain-containing protein"
                     /protein_id="HEX9916855.1"
                     /translation="MNHKRKFFDSHAKEWDRKNHLGDPMKLKELVSIFKIKKGAKVLD
                     IGCGTGVLLPYLSKNVGENGRVFALDFCWEMIKKAKRKKIAKNVFYINADTNDLPLAG
                     DSFDYIICFATFPHLDKKLKSLKEMQRVLKPRGKVFISHLASRNEINAFHKRSSKVVS
                     NDILPTEKNMRRMMTKAGFKNTKVIDKPSFYLAYGEKR"
     gene            complement(17280..18131)
                     /gene="prmC"
                     /locus_tag="VGB16_03885"
     CDS             complement(17280..18131)
                     /gene="prmC"
                     /locus_tag="VGB16_03885"
                     /EC_number="2.1.1.297"
                     /inference="COORDINATES: protein motif:HMM:TIGR00536.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008757 - S-adenosylmethionine-dependent
                     methyltransferase activity [Evidence IEA];
                     GO_process: GO:0006412 - translation [Evidence IEA];
                     GO_process: GO:0018364 - peptidyl-glutamine methylation
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="peptide chain release factor N(5)-glutamine
                     methyltransferase"
                     /protein_id="HEX9916856.1"
                     /translation="MSKVCLEVLQEAKAKLKKKKAQTAQLEAELILSSVLKCERIKLH
                     LDPKRVLTKAEHKLFEKLLKQRLSGKPLQYTLGEAYFFGYKFKVNPNVLIPRPETEIL
                     VSEVISLLRTKENSKIIDLGTGCGNIATGLALNLKDAFIFATDISPNALKVAKLNAKL
                     NRVEDRIKFLCGDLFKPIKTKFDAIVSNPPYVSEKEYTKLPDEIKNFEPKEALLAGKD
                     GLEYIKKIVEQAPGFLNKDGILALEIGFGQERKVKELVFNSKFLKLIEIKKDLSGIPR
                     VMLARKL"
     gene            complement(18124..18375)
                     /locus_tag="VGB16_03890"
     CDS             complement(18124..18375)
                     /locus_tag="VGB16_03890"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEX9916857.1"
                     /translation="MKRRDFIKYISLSSLGICLIPRQALGSLADKRAKIKPLNCSSHW
                     LIGSPVKGCSTSNLFVECSGSIQNGLDDFNHFRLPEVYV"
     gene            complement(18372..19706)
                     /locus_tag="VGB16_03895"
     CDS             complement(18372..19706)
                     /locus_tag="VGB16_03895"
                     /inference="COORDINATES: protein motif:HMM:NF039907.3"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008270 - zinc ion binding [Evidence IEA];
                     GO_process: GO:0006508 - proteolysis [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="TldD/PmbA family protein"
                     /protein_id="HEX9916858.1"
                     /translation="MTSQKKILDLFEDSLVSSSADQTELVLMADDLNLSRFAENIIHQ
                     NIAQSDHNIFARVVLGKKLGISSTNRLEQKEIEKCINQAIKIAQFQKEDPNFVSLPKP
                     GQRIENRLLHEQTFSVDPRDKALRIEGIVKKCKADNLFSAGAFQDKKEALAVVNSLGI
                     KGYFKRASTDISLTVNDKDQNCGWAQDFNWNAKNLTVENVGQTAIQKALLSRNPIELS
                     AGKYTVILEEAAVANLLLFLSFLGFGAKGFIEKRSFMAGKIGEKLTGDKITIVEDPYS
                     PENPGIPFDYEGVDKKRVVLIENGVGKGVVYDSYYANLAGLESTGHALPPGNNFGPYP
                     QNLVMNEGDSTEEELIASTEKGILVTHFWYLNYMNPSKTMVTGTTRDGTFLIENGKIK
                     SGVKNMRIGQSILEAFSNVQMMTKKRKLCPQYGVLLFVPQMKIENFNFLPNQ"
     gene            complement(19703..21151)
                     /locus_tag="VGB16_03900"
     CDS             complement(19703..21151)
                     /locus_tag="VGB16_03900"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_020252482.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008270 - zinc ion binding [Evidence IEA];
                     GO_process: GO:0006508 - proteolysis [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="TldD/PmbA family protein"
                     /protein_id="HEX9916859.1"
                     /translation="MMDFKKACQIALDCSKSFGASYADIRIEQTENETVSVKNGTPEP
                     INCSTSLGFGIRVTANGSWGFASNPDLTAKNIKNTAKLAIEMAKAASLLAKEDVVLSP
                     LEKIVDTYLTPIRKNPFEVSLKEKIEYLLFLDQLMQKVQGANITNSFMDFSRTNKIFS
                     SSIGSFLEQTIYQSGAGIEATAMQGGHKNMQTRTYPQNSGQYQTKGYELIEELGLQEN
                     ASKIAQEAVALLSAKDCPQGITDLILDGSQLSLQIHESIGHPLELDRVFGSERNFSGT
                     SFATPDKLNNLKYASEIVNVINDSTYPGGLGTFGYDDEGVKANKSYLIRGGLLVGYLG
                     SREYAAKIAKESNGCMRAQGWQNLPIVRMTNTNLLPGNKTLEQMIAEIDDGIFMSTPA
                     SWSIDDTRDNFKMGCEIGWEIKSGKLGEMIKNPSYSGNTIRFWNSCDAIANENYWKIW
                     GTPNCGKGQPGQIMTVSQGASPVRFRKVSVGE"
     gene            complement(21148..>21480)
                     /locus_tag="VGB16_03905"
     CDS             complement(21148..>21480)
                     /locus_tag="VGB16_03905"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEX9916860.1"
                     /translation="LNKDALNRLAWATLFNAALTFIGGIIIGAWVTHYTFGGYWEGVP
                     IGWDITDNKTLIILLYWLIMLFLMKGTIFKKDGKLDLLKPKPLAIFTLIGVILTIFLY
                     LVPHSIRL"
BASE COUNT         6023 a         4590 c         4656 g         6211 t
ORIGIN      
        1 gccctcgcct cgattcggcc cgcctcggcg agccatcggc gagacgggcg aagcgggcct
       61 ctttttcaaa gagggggaaa agaggtctcc ccttttgaaa aagggggatt gagggggatt
      121 ttaaaataga ttgcatattg ctctaatatg taacaacatt taatgcgttt gctataatca
      181 acgctaaggt tttcactaaa atcataagaa acctttttgg tgaaaaccga gtgtagggac
      241 aggtcgcgac ctgtccctac caatgcacca ggtcgcctcg cttctaatgc ataatttggg
      301 ttaaataatt cctctataaa agtgcctcgt ttttgtcaca aaaaagttgc ggattaattt
      361 aacttctatt atattaataa tttataaaag agaactatta tgagtaaacc gcagccattg
      421 ggcttagtcc tatccaaaat tctaaaaaca agcggtatgg ataaaggaat aaaacaaagg
      481 caggcaattc tcatctggga caaagtggtt ggtcagaaga tggccagcca atcaaaagcg
      541 atcaaggtag aggattcgaa gatatttgtt aaggtgaaaa atccggtatg gagaagcgag
      601 attcttttaa tgaaaagcca gatcgcggag gcattaaaca aaaacttggg tcaaagggtc
      661 ataaaagatg tgatagtgtt aggggattag atttttagaa tacaaggaga gctcctttat
      721 ggaaaaagag gaagacagga aagacacaag cctaaaaggg caccattacg acgccaccac
      781 cattcaggtt ctaaaagggt tggaggcggt gcgccgcagg ccagctatgt atatcggcga
      841 taccagcacc agaggattac atcatttagt ttatgaggta gtggataaca gcgtggatga
      901 ggctctggcc ggtttttgcg accagataaa ggttagtata aacaaagata acagcgttac
      961 agttgaggat aatggcaggg gaataccagt ggatatgcac cccacgcaaa aaaagcccgc
     1021 cttggaagtg gttatgacta tgctgcatgc tggagggaaa ttcgaccact acacttataa
     1081 ggtggctggc ggcctgcacg gggtgggagt ttcagtggtg aatgcccttt cagaatggtg
     1141 cgaggtggag gtcagtcgaa atgggcaggt atattttcaa agatatgaaa agggtaacac
     1201 cgcaacccca atgaaggtca tcggtaagaa gaaaacttgt ggcaccaaga ccacctttaa
     1261 gcccgattcg gaatttttca aggatatgaa cttctcttac gaaattatct ccgcccatct
     1321 aaaggagctg gctttcttaa acaaagggct caaaatccaa atagaagata aaaggagcgg
     1381 gaaatcaaac agtttcgaat atagtggcgg aatagtctct tttgttgagc atcttaatga
     1441 gaacaagaac ttaattcact ccaagccgat ttactttcaa catcagaagg agggggtgga
     1501 gctggaggtg gctctgcaat ataatgatgg ctacgtggag aacatcctct cctatgtgaa
     1561 taacatcaag accattgaag ggggaaccca tctgatcgga ttcaagaccg cgttcactcg
     1621 aagcatcaat agctatgccc agaaaaataa tctcttgaaa aactccggct tcagcttgga
     1681 gggggaagat gtgagggaag ggttgaccgc ggtcatctcc ttgaagattc ctgaccccca
     1741 gtttgaggga cagaccaaaa ctaaattagg caacagtgaa gtcaagggga tcgtggagca
     1801 gataaccgga gagaagctaa gcgaatatct ggttgaattc ccttcagttg ccaaaaaaat
     1861 tacggagaaa tccatctccg cggctcgttc cagagaagca gccagaaaag ccagagacct
     1921 tacccgcaga aaaagtgctc tggaatcagg aagacttcca ggaaagctgg ctgactgttc
     1981 cttgaccgac ccggagcatt gcgagatata tattgtcgaa ggtgattcag ctggtggctc
     2041 tgccaagcag ggaagggaca ggagatttca ggcgattttg cccttaagag gtaaaatctt
     2101 aaatgtggaa aaagcgcctt tggataaagt tctttccaat gaggagataa gaacgctaat
     2161 caccgccatc ggcaccggaa ttggtggaga tgagtttgat atctccaaag caagatacaa
     2221 caaaatcata attatgacgg atgcggatgt tgacggctcg catatcagaa ctttgatttt
     2281 gactttcttc tacaggtata tgaaagactt aattgagcag ggcaggattt atattgctca
     2341 gccgcctttg tatagaatca gaaaaagcaa agagcagcat tatgtttatt cggatgatga
     2401 aaaagataga accatagccc gtttgggcgg tggagatgga gtggagattc aaagatacaa
     2461 aggtttgggg gagatgaacc cggaacagct ctggcaaacc actatggacc cggaaagaag
     2521 gacccttttg caggtaagtt tagaggatgc ggctgaggcg gagcggattt tctccattct
     2581 aatgggtgac caggtcgagc ccagaaggca gtttattgaa gagaatgcga aatacgtgag
     2641 aaatttggat gtataggtat ggttttgtca ttgcgaggag ttcatatgga cgacgtggca
     2701 atctcggagt tgctttagat tgcttcgcta tcgctcgcaa tgacagacaa aatcattttt
     2761 gtagttgtcc taatttcatc gggaggaaag aatctagatt tattgtcatt cccgcgaagg
     2821 cgggaatcca gatttttcaa aaacaaatgg atttctgcac gagttctgtg tcttgagatt
     2881 ggaatttatc ccgcttgaag cgggacagga atgacagata accaagaaaa caaaacgtga
     2941 aactcaaacc caaaataatt ttatttattt tcttagggct ggttttgatt ttggctttcc
     3001 ttcgtctttt gtccccggag gattgttgga ttaaagacaa acagggaaat tgggtaaagc
     3061 acggaaatcc gtcaggacct ccgccaaggg ggaagtaaag aatgtccaaa attattatct
     3121 ggctgaaaac ttatctgtat ttgatattgc ctgcaatagt tattttggcg attgttatag
     3181 tttcaatagt tttttgtcta tccgcttcga agattattca ggcttcagtg gcaggtggaa
     3241 ctttcctttt ggcaatagcg gcattcggca gtatagcaga aagcagaaag caatttagaa
     3301 taagcaaaag cccgattttt gatgtcatga tagggggcca aataattaac aatagtaatg
     3361 aaataaaact tcccctgtca tttgagaata taggctatgg tacggcattc agagtgtcta
     3421 ttgaattaaa acaaggtgct ttggcatgga atctcgaatt tcccttaaat gagtttcagg
     3481 aaataggtat aggttatgcg tggacagtcg gcaagaagaa gactatgacg ttcagtccta
     3541 gagagattgc acctgcacaa atagaaaaac cattagaact cgagattaag tgcgataaca
     3601 tttttaggag gagggttgtt cagaagttct cgggcaatat gaagcatggt ttcagaatag
     3661 aaaatataga gtttgcttaa taaatagctt ccgtgcttgt cgcgggtccc tgacccgaca
     3721 agaaaaagtg tcaggagtag ctcctgacac aacgacaaaa tagataatat ttcttaaaac
     3781 cataaaagga ttcccttatg ccttttgaat ctgaaaagat agttccggtt tttttggaag
     3841 aggagatgaa gtcctcttat atcgactatg caatgtcggt tattactgcc cgtgctttgc
     3901 cggatattag agacggactt aagccctcca atcgtcgtat ccttgtagcg atgcacgatt
     3961 tgaacttggc tccgaataaa gcccatcgta aatgtgccaa aatcgcgggc gatacttcag
     4021 gaaattacca tccgcatggc gagcaggtgg tttatccaac tttggtcagg atggctcaag
     4081 atttcaatat gcggtatttg ttagttgatg ggcagggtaa cttcggctca attgatggcg
     4141 actctcctgc agccatgaga tacactgaag cacggttagc tcctctggct atggagatgc
     4201 tggcggatat tgaaaaggag acagttaatt tcgttcccaa ctacgatgtc acaagagaag
     4261 agccaacagt gctccccagc aagttcccca atctgatttg caacggctca tccggaattg
     4321 cagttggaat ggcaaccaat attcctccgc ataatttagg tgaaattgtt gatgctatgg
     4381 tagctttgat ttccgattca gacgtagaag atgagaaact tttagaatat gttacaggtc
     4441 cggattttcc aaccggagga atcatttatg gcacagctgg aataggggaa gcgtacaaaa
     4501 ccggtagggg aaagattatt cttcgggcta aggcaaatat agaacggcag aaaagtggca
     4561 aggaaagcat tgtcatcact gaaataccat atcagataaa taaatccaac cttctggaaa
     4621 gaattgcgga gctggtgcaa aataaggaga ttgagggaat ctctgatttg agagatgaat
     4681 cagatagaga tggaatgaga gtcgtaattg agcttaaaag ggaagctcca gcagaaatcg
     4741 tcctaaacca gcttttcaag catacccaaa tgcagaccac cttcggaata attatgctgg
     4801 ctttggcaaa gggtgagccc aaggtattga acttaagaca gatgttagat ggtttcatcc
     4861 agcatcgaca cgaggtggtt acgcgcaggg cgaaatttga cctcaaaaaa gcggaggaga
     4921 gagcgcacat cttagagggt ttgaaaatcg ccttagataa tatcgatgcc ataataaaat
     4981 tgattagagc ctccaaagac gttgatactg cccgtcaggg gttgatgaag agtttcaaat
     5041 tatcccagat tcaagcccag gcgattctgg atatgcgttt gcaaagattg accggcctgg
     5101 aaagaaagaa aattgaagaa gaatacttag aattaatcaa ggagattgcc cgcttaaagg
     5161 gaatcttgga aagcaaagcc aagaggatgg caatcataaa agatgagctt ttggagttaa
     5221 agaaaaaata tgcggatgag agaagaaccg aaataaaaga ggcggaagag gagttcacca
     5281 ttgaggattt aattgcggaa gaagatatgg tcatcaccat cacccactca ggatatatca
     5341 aacgtttaag cgtgagcgcc tatcgtcgtc agggccgagg tggcaaggga gtaatcggca
     5401 tcgagaccaa ggagaccgat tttgtggagc atctttttat cgcctccacc cacgactata
     5461 tacttttctt tacccaaaaa gggagatgtt attggctcaa agttcacgaa atcccggtag
     5521 ggggaaagat ggcaaagggc agacccattg tgaatatgct ggagctttcc gaagaggaga
     5581 aggtggccgc atttgtcccg gtaaaagagt tcgatgataa gcattatgtt ataatggcta
     5641 ccaagaatgg cacaataaag aagaccgtgc tttccgctta tagcaatccc agaaagggcg
     5701 ggataaatgc cataggtatc gatgaagatg ataccttaat cgaagccaaa attactgatg
     5761 gcaattgcga tatcgtcatc gccaccaaag atggtcaagc cataagattt ccggaaaaca
     5821 aagtcaggca gatgggaaga acagcttacg gagtcagggg aatctctttg gaaaaggggg
     5881 atgaagtaat tggtatggtg gtgataaaaa gggataccac acttttgtcc gttacggaaa
     5941 acggttatgg taaaagaagc cagatttcag agtatcgggt gaccaatcgc ggtggcaagg
     6001 gagtaatcaa cattaagacc actgaaagaa atggcaaagt ggtaagcata aaagaggttc
     6061 aggacgagga tgagctgatg cttattactc aaaaagggat tgtcatccgc cagcccataa
     6121 agactataaa aaccattggc agaaacaccc agggggtaag attaatcaac gtaagccagg
     6181 gagatagagt ggtggatgtg gcaagagtag tgaaaagcga aggaaatggg aatggggaag
     6241 gaaacggggg agagggaaca gctgacgcgg tgaatggaca gtaaggaaaa tttatttgtt
     6301 tgttaattcg ttagtttgta ggggcgggcc cctgtgcccg cccctaatta atttccgagc
     6361 gggcgagaat acctcgcccc tacatttttc cattatattg gtccataacg acccatacca
     6421 aagaaggcga cccatatggg tcgcctgcgt tatttgtttg gtggctttga tttagatcgg
     6481 gaagagagtg ttctgaaatt cacttagttt actctgctgg gttggagaat aggtggggat
     6541 ggtttatttt tgagttaggg ccgtatattg atacactcca tcttggattg ttcccaaata
     6601 tagtttgtca acctttccat tacttaaaag caaaaagtcc cttacatcga gagtcaagcc
     6661 cgcatcttga ctgataggaa accacgaaca gcccgcatcc ctggttttat ataagatcaa
     6721 aggttgagga gtgggtactc ctcctgtcac atacaccact gacggatagt aaggattgat
     6781 ggctatggca aatagataag cggaaacttc ttccaggacg atattccaaa tagtgccgcc
     6841 atcgatggtt ttgatgactt tcccttccat gctcacataa actacgtcgg agtttgatgg
     6901 atcaatggca atacctagac atgcattgtc accgccaaca tcagggtagg ttgtttgcca
     6961 attctcacca cggtcagttg acttaacaag atatgctgca aaaaaaccag actccccgcc
     7021 tgcccaaaca gtgtttgggg aagcagaatc aactgttatg aaatgagtcg ccacaccccc
     7081 ataatgccat cctccccaca ccctgcgcca actcgagccg ccgtcagtgg attttgccac
     7141 tacacaagct cctgtggcaa agagcgtatc ggattgacca ggaaattttg ccagggccat
     7201 aacctgatgg gaccctcctc cgccaaatcc gttttgaaag gcctgccagt ctgttccccc
     7261 atctgttgtt cggaaaagag atagtgtatc actacccacc tcggtgatta ccacgctggc
     7321 aagaattgta tcggggtgaa caacaagcaa tgccacagca tgcttgccct ggagcccgat
     7381 cggagtccat atagtatcag gtgaggtgat atctttcctg tagactccat catccgtgcc
     7441 tgcataaaga acgttgccct tgagccttaa cttgtgtatc cacctcccgc ctaagccaag
     7501 gcatttccac tcctgttgtt ctacgggtga gtgaactctt tcagaacagc tcaccaagaa
     7561 aataagaaca ataagcaaaa ggactttgta tttcatgttt cgatcacact ttactttcat
     7621 tctcgatagt caaaagagaa ccgcacccaa tttcaattgg tggcactgca attatagata
     7681 ggaagtcttg ttttgtcaac ttgagattgg gataaggaat gcagaaacct gcatcggtaa
     7741 ttgtctgggg tgagtttgaa tgtaagtagc tctaagtaga ataagaactt attaagttgc
     7801 agtactttac gttccctcta ttagaatggc gaattgaatc aaaagccctg gccaggtttg
     7861 acttgtttgg tggttttgat ttggatgggc aaagggatat tttagggatt acgtaggtgg
     7921 tataggaatt tatgtgtttg cgcgtagggg caattcatga attgccccta cattttgcaa
     7981 tgacaaatag aaccgtagac gacattccgg aatgtcgtct acgctattgt aggggtcgga
     8041 ttcatccgac ccgaaatgtt attataaaaa cgggctcgat aaatcgagcc cctacattta
     8101 ttgttttata tagggcaacc ctttggggtt gcttggtttt tcttattata tagataagtt
     8161 gttttttata tctttccaag aaatttcttc tagctttgtt tgcaaaaatg ttagtttgtc
     8221 atcgtttatc caattatccc atttgtttga aatcgcaccc cttgctgcat ttttgagatt
     8281 tgggttagga tcgattacgg tgattttatt cttttgtgaa gaatatagcc actgaataat
     8341 ctgtgtgttt attcctttgt caccaaaacg atagcctatt ataacaagcc tttcggcatc
     8401 gtgtagggat ttatagaaat aagtgaaaag actagtgtaa attcctcgta aatattctaa
     8461 cattttattg aatgtcccta ttaaaagcaa cggtctccca tctaccttgt caatgtgtga
     8521 gtatgttata gaattcaatg gaataccgat tgattcttca taccaacccc cgccagcagg
     8581 tctgagtcgg aaccaatcca ccgagccatg aagtttgaat aaatgtatct tacaggcaga
     8641 ggtttcaaaa agcttgttgt cccaatatct aacaccattt atcggttcgc caaaaccatc
     8701 attaaatgga atattatttt ggcataagca ttgttctaaa acagtatcgt gattcagtgt
     8761 aaatatgtct atgtgagata aattgttatc tctcgatata tcctttacca aatttaaata
     8821 atcaaggcga tttggttttt tggaaagcat attccacgct atatctgcta tataatgtgt
     8881 tgtctcgtca gcgagttcat gaaggcgcca gttttttcta gtttcacctt ctttaccttg
     8941 gagaagagat tcaacattag cccggatttt atcgataaat ggttgggcta ccggattatc
     9001 aagttcctca agttcgctgt catgaatttg gcttactaca taataaatgt cctcataatt
     9061 cgtttttcgt tcagggttat aattataata ccgaccaatt tcgggcataa gtatttctat
     9121 aaagttcttt actcttggaa catattcgtc tggaatgcca acgtgggcat ataaaggctg
     9181 accaaagtaa tagttaccat ccgtatggcg cattatgtta ttagcagata gaacttggtc
     9241 ggtaatatct ttggtcgatg gcattcctgc tggaattgaa actcctgaac caaacaagaa
     9301 gaccaatttt gatgataaag gaggtatcac tctacccctt cctgctcccc ggcttataaa
     9361 ttggctcgcc ggatttgcac aagggacatt cttccggttt ataggttttg gcggagacgg
     9421 tgacaagtgg ctttaaatcg taatccattt tgactgttcc tccgcttcgg tccaaaaata
     9481 ctccgattcc gacgatatac gccttgtatt tcttaaccaa actaataact tcaaaaatgg
     9541 atttgccggt agttaagata tcatccacaa tcaaaaccct ttgcccttca ttcaaagaaa
     9601 accctctttt gaaaactctt tccccttctt ctctttccgc aaagatggct ttaactccta
     9661 ggtttttcgc cacctcatag gcgacaatta ctccgcccac agttggtcca accaccaccc
     9721 cgattttctt atcctcaaac ttgtctgcca atttcttgca caacatctcc acatagtgag
     9781 gatattgcag gacttgaaac ttctcgaaat attcgtcgct gtgcagtcct gagcttaaaa
     9841 ggaaatggcc ctttaaaatt gcgcctgatt ttgtaaagat ttccaaaact tcgttttgct
     9901 cggacataaa atctcctcgg aattatattt ttaaattggt ctatattgtt cgcgtagggg
     9961 cgaagtttcc ttcgactttg ctcaggacgg tgagcctgtc gaaccgtatc ttcgcccgag
    10021 gggcaacggg tggagacaag ctccaccgct acgcgcattg tttttgtaat tgccctgatt
    10081 tatcaggttg ctacaatttc aaaactttaa gccgtaaact caaaactgct tttagattgc
    10141 ttcgccctat gggctcgcaa tgacagtatc acacaaacca tagtgtcatt gcgaggagcg
    10201 gagcgacgaa gcaatctcat tttcatgtag ggatagagca ctgttctgtc cctaccgcat
    10261 taacctctcc ccagccctct ccttatttaa ggagagggaa caactaattt tcatttttct
    10321 cttgcctttt tgaatttccc ttttaacttt gatacgaggg aggatagcat ttgaaaaacc
    10381 tttaacagtt agctgatgaa ggttgtcccc ggttgttgcg cgtacgcggt gaccttctct
    10441 cgattttatc ggggggaata gcgaaagcaa aacacgactg gcgactataa aaaaccttta
    10501 gcctactagt gtggaagagt tctttcaaat tgcatatcct ccccttattt attatatatt
    10561 ctcccttata ttctggaaaa caaaatagaa tcccatctcc tccaagcttt cctggtgagg
    10621 agaagttatt tttttcaaat cgagcaccct ttctttttta aaagatagtt tttccagatg
    10681 ggcagcccat ttggcaaaag ccataatcac ctctccactc cacgtgcctt ctttgccctt
    10741 aatcaccgag atattagcat tgttgtccac gaagagattt gccataaaaa gatatgaagt
    10801 gactaacttc tccttggctt gtcgcaagtt ctgtgtggtc ctataaggac cgagcttgtc
    10861 cgacacaaag cgcgacaaaa ggttcattcc ttctattagg agatttacct tcttttgttc
    10921 caaaggcggg aaattcagtt tccccacctc ttcggcaccc agtatctttt ccaggcgcaa
    10981 taaacttgcc cctaaaacca gattcaaaag ggaaaagttt aaatccggac gagcgagaat
    11041 taaaatcaaa gcagagggtc ggcttaaaat tatggcactg ccattagcgt aaaataattt
    11101 gacctcaccc tgggatttca tatcctggcc gagcttaatt atcttggagc aggtggattt
    11161 gatggcgctg gtggcgaaag aggcaggcag aagctctgaa gacctcaaag tcttcttgtc
    11221 ccagataagc acaccaatta caccctgaac tgcttttatt tcctctaaga ctttttgcat
    11281 aggttcactc gcctctgcca gaaagtttcg ctcgcctctc ggcgaagccg agggcctttc
    11341 atattcggga agccccgtga tttatcatgg ggaggcttca cttcaaggca ttttctattt
    11401 tgtgtatgac cgcttcaatc tctttttcct tattcagatg cacagcaaag tagttgtccc
    11461 ttctttgaaa gaggattacc ttgaagtcat ctgtctgtat aatgcttttg ttgaaattgc
    11521 caaatttgga cagctgtgat aaaaagttgg ctaactgaat ggcagagatt acattctcgg
    11581 taatcttttt ggatacgtcg ctctcccttt cttgatacag gacttttcct tcctggtcaa
    11641 aggcaaaagt gttttcaatc ccttccatct tggagagttg ttcccagaat ttgggcaatg
    11701 gttttggcat atgatcggcc aagatctcct ctgccttatt tctgaccacc gatctgacct
    11761 gcttatgctg ccttcggatt aaactctcaa gtttcttctt atcctcttcc cggcgcaagc
    11821 caccttccca ggcctcctca actttatgaa tgacctctcc ttcacaggac acggttgtgg
    11881 ttatacgagg ttgtggcctt aaggcaaatt cagtttgcag atagagcttt ttttctttcc
    11941 aggatagata agaggaaatt ccagctggtt ggatagagct catttttcac cttgcttgta
    12001 gaaaggataa gctaattgaa cttgcaaaaa gggatatacc ttaagaggca tatccttttc
    12061 ggattttata ctgtcttgta acataaacag aggtttaatt taaaaatcaa agccggggtg
    12121 ccccacagca aagctgtggg ttaccagcat catagactgt attagcactt ggagtagccg
    12181 caatgtctgc agacattaca tccctccttg tgctctaatc tgctgccaca gtcggggcaa
    12241 agatcaactc ccaaatccgg gtcggagatg tggttcttgc ctgagccaaa atgcttgttc
    12301 agaactttgg ctatggcatc cgggatagac ataatcaagc cgccattctg gaacacaggt
    12361 gaggcaccgc cgattccgat taattgctcg attatcctat gcatactcac tccgcttctt
    12421 agggccaaag agataagtcg gcacacagct tcagtatccg ccatagtgga ataaccggat
    12481 ttgccgatct gggcaaagac ctcaaaaggt ttcccctcca ggatattcac tgtaacgtag
    12541 agattgccat aaccggtttt tatcttgtca gttattccgg ttaaacagtc cggtctttcc
    12601 tgaaccttaa ccttctcctt tgactctttg cctttcttgg tggagaggac ctggtcttct
    12661 cttgagccat cgcgataaat agttactccc ttacatccca attcaaaagc taacttatag
    12721 gcgagttcaa catcttctaa tttggcttgg ttaggcaagt tgatggtttt ggaaacagcg
    12781 ttatccgtat gcttttgaaa tgcggcttgc atacgaatat gccattccgg gctaatctgg
    12841 tgagaggtaa caaatacatt cttggtctcc tctggtattt cactaaaccc cttaagggag
    12901 ttggtgttag ctatcttctc catcagctcc ggagaataaa aacctttagt ttgggcttct
    12961 tgcttgaaat agggattagc ctcaactaat tttgtattat ccatcactgt tctgatatag
    13021 gatactgcga aaagtggctc gattccagaa gagcagccag caatgatgcc gatggtgcca
    13081 gtgggagcaa tggtggttac tgcggcattt cgtataggca tcccttcttg cctgtagatg
    13141 ctttttcccc aattagggaa atctcccctg actctggata aatccgacga agctttatga
    13201 gcttcatcct ggacaaaaaa cataacctct tcagctaaat ttactgcctg ggaggagtca
    13261 taagggattc taagctgaat caacatatct gcccaaccca ttactcctaa ccctatcttc
    13321 cgattggcca aagtgttttt ctctatctgg gacaggggga acttgttggc gtctatgaca
    13381 ttgtctaaga aatgaactgc cgcgcgcaca gttttggcta atttgtccca atctatttca
    13441 tatctaaaaa ggttggcgtg ttcagaatca accactgcct taagcatcaa agacaaattg
    13501 actgaaccca gattacagct ttcataaggc aaaagtggct gctcaccgca ggggttggta
    13561 gcttcaatct ctcctacatt aggagttggg ttatctcgat ttatcctatc gataaaaact
    13621 atacccggtt caccgcttct ccaggcatgc tccacaatct ggttaaatac ctctttggct
    13681 cttaatctta ctggcttggt gtccctgaaa tattcctttt ttgtgcgggg gtcaattaaa
    13741 ggataatact catccctgtc cactgcccgc ataaaatcat cagttactgc tacggagatg
    13801 ttaaagttgt tgagttcgga gaggtcgtct ttagaggaga tgaactccgg tatgtccgga
    13861 tggtcaattc tcaagacccc catattcgcc ccacgtctgg tcccaccttg tttcaccgcc
    13921 tcggtggcgg agttgatcac tttcataaaa gagatgggtc cacttgccac tccgctggtg
    13981 gaagctacca cggaattttt gggtcttaaa cgtgaaaaag agaatccggt tccaccaccg
    14041 cttttatgaa ttaaggcagt atctttgact gcggtgaaga tggactccat tgaatcttca
    14101 atcggcaaaa caaagcaggc ggaaagctgt cccaggggcc ttccagcgtt cattagagtt
    14161 ggagagttgg gcataaactc cagattggtc attatctcat agaagtttct ggctatctcc
    14221 tctgtttcct ccgggctggc tccgtacttc tcatccggac cagcgataaa gcgagccacc
    14281 ctccagaaca tctcctccgg agtctcgatt atctttccct catcgtcctt ggcaagatac
    14341 cttttctcca agaccttgag agcattctcc gtcaacttca attcattttg tatctttttc
    14401 tccatctatt ttccccttag tcccccgaaa gcaattttgt ggcaattttc tttatttatg
    14461 ttagatttcg ttaattggct atttcaaata gcgtttaata attttttatg aagcgaccat
    14521 tcagacaata tgtcattccg cacttgatgt tgaaaaccca gtagttaggg cggaatccag
    14581 cttattgctg ttttcttttt tggattcctg cctgcctagc ggctcgcctc acggcgaagc
    14641 cgtgggcaag gccgctggct ttcgcaggaa tgacaaccaa aaacctcacc tttgtaggtc
    14701 gggttgcgtg tcattcaacg agctacccga cattcttgat ttatttgtcg ggcacccttc
    14761 aggagcccga cctactttta acccctcctg acctcccctt atcaggggag gaaaatggtt
    14821 tgtgttctcc tgatagtcaa aggaggaacg ctttgcgtcc ccctgataag ggggacaaca
    14881 gggggttatg ttgtcaaaca tcctttggat ttatcaaaat tctaacttaa tctatcttcg
    14941 ggcgaagata aacttcgccc ctacgcggaa accttttatt gtaggggtcg catttatccc
    15001 gataaatcgg gacaagttac cacccgcggg ctcgatgtcg tcaagatgct gcgcaaatcg
    15061 agcccctaca gttccttcct aaccgccact ctcttttttt catccacctc aaaatcccaa
    15121 aaaacaatcg gcgccttctt tttcataatc ttcaacatct ccagacaaat ttctcttatc
    15181 tcccaatgcg cccgctcttg acagcgaact tgaaaaatat gtcttagctc tcgaaagttt
    15241 gcagttatca caatttccga agttgtggca ttgggaagta tgtatctggc gtcctcttta
    15301 ggtatatcgg ctttcaccat ctgggagtat aattcataag atttcttagc aaaatttgta
    15361 aactcttttt tgaatttcgt cttttcaatt gtgggtggaa ttacaaattc aaactctttg
    15421 gtcttgccgt attccacata tctctggctc tcctgcgaag gggaaagcaa tctgtgtctt
    15481 actagctcat gagtgaaaac ccttgaacca ccttttatcc tgaaagttgc ggaggcatgc
    15541 tccaaaaccg aatgatgccc tttgcgtatg acttttttta tcaactcgcg agtggaggat
    15601 ggaggattgt agcgatgaaa gctcaaatag caggtgcggc aagccctctc aatcaaatct
    15661 tcagccttgg gagttatgga gataagctct actttcatat tcttcttagt ctttttaggc
    15721 gacacttctc cggccggaga cgatttctcc gaaaagcata atttagattt tagatttata
    15781 ataactcggg aagagtctat tgttcaagtt ttatattttt cttccgcacc taaattagtt
    15841 tcaaaaattt tcaagtcaag ggaaaattta ataaattttc aatatgttgg atagcaatca
    15901 tttaaaaacg ccaatatgtt gtggttacat ctctgttcga gaagccagct gaattccctt
    15961 cactaagatc ggtactagaa ttatctgtaa gattattccc ggtagcccag ttggaatagc
    16021 aaccagtata aattctttaa tcccataagg aagattaata aatcgtccca aaataagaag
    16081 agcaaaagca aaacccaggc gtccaacaat cattgccaag agcagcccaa caattagagg
    16141 aattcttaag gttctcataa gtatgccgct ggcaaaacca tataaaacca actccaaagt
    16201 catcaaagga actgcatagg ctggtggcat tccagtcaga agaaaactgg cggtagggca
    16261 gaccaagcca actattattc cgcttattgg tccaagcagg atgccggcca agagaaccgg
    16321 gaggtgcata ggcaaaagag ttcttccagc taagggatta atgaaatgaa aggcaattgg
    16381 cagagccaca cccagggcga taaaaagccc ggtcaagctt atttccctat ttaaaaattt
    16441 catctctttt ctccataagc cagatagaaa gatggtttat caattacctt tgtgtttttg
    16501 aaaccggctt ttgtcatcat ccttctcata tttttttcag ttggcaagat atcatttgat
    16561 actactttac tcgacctttt atgaaaggca ttaatctcat tacggctagc cagatgtgaa
    16621 atgaaaacct tccccctggg cttaagaacc ctttgcatct cttttaaaga tttcaatttc
    16681 ttatccagat gggggaaagt ggcaaagcag atgatgtagt cgaacgaatc cccggccagg
    16741 ggcaaatcat tagtgtcagc gttaatataa aaaacattct ttgctatttt ttttcgcttt
    16801 gccttcttaa tcatctccca gcagaaatcc agggcaaata cccttccatt ttctccaacg
    16861 ttttttgaaa gataaggcaa aagcacaccg gtgccacaac caatatccaa taccttagcc
    16921 cctttcttga ttttgaatat cgaaaccaat tccttaagtt tcatagggtc gcctaagtgg
    16981 ttttttcggt cccattcttt tgcgtgggaa tcaaagaatt tacgtttatg attcatgatt
    17041 gttttttgta ggggttcaaa attttgaacc cctacgctgg ttacttctct ttctgttttc
    17101 gcaatctatt gcaggcgaca ttcgcgaatg tcgcctactt gttttgtagt tgcccaattc
    17161 attgggcgca ttttaaaaat cgctcgatcc ttcgacaagc tcaggatggt gacagttcga
    17221 cttcgctcac tgtccctgag caaggtcgaa gggagtctgc cgaaccataa atcgagcaac
    17281 tacaattttc ttgccagcat cactctcgga attcctgaca aatctttttt gatttcaatc
    17341 aatttaagaa atttactatt aaagacaagc tcttttactt ttctttcttg tccaaacccg
    17401 atttccagag ccaaaatgcc atctttgttt aagaagccag gggcttgttc gactattttc
    17461 tttatatatt ctaacccatc ttttcctgcc aacaacgcct cttttggctc gaaattcttt
    17521 atctcatctg gaagtttggt gtattccttt tctgaaacat aagggggatt agatactatg
    17581 gcatcgaatt tggtttttat tggtttgaat aaatctccgc ataaaaattt tatcctatcc
    17641 tcaacccggt tcaatttagc attaagctta gccactttca aagcattggg ggaaatgtca
    17701 gttgcaaaga tgaacgcatc cttcaaattc aaagccaatc ctgtagcgat atttccgcag
    17761 ccagttccca ggtcaattat tttcgaattc tcctttgttc tcaaaaggga aattacctcg
    17821 cttactaaaa tttctgtttc cggacggggg attaagacat tgggatttac tttgaatttg
    17881 tatccgaaaa agtaagcctc tccgagagta tattgcaagg gcttgccgga aagtctttgt
    17941 tttaaaagct tctcaaaaag tttatgctct gctttagtta aaaccctttt tgggtcaaga
    18001 tgaagcttta ttctttcaca cttcaaaaca gaagataaaa taagctccgc ctcaagctgg
    18061 gctgtttgcg ctttcttttt cttgagcttc gcttttgcct cttgcagaac ttcaagacaa
    18121 actttagaca taaacttcgg gcagtctgaa gtgattgaag tcatctaatc cattttgaat
    18181 tgaaccagag cactctacga aaagattaga agtggaacat cctttaaccg gagagccgat
    18241 aagccaatga gaggagcaat ttagtggttt aattttggcc cttttgtctg ccagagaacc
    18301 cagggcttgt ctaggaataa ggcagattcc caaagaggaa agagaaatgt attttatgaa
    18361 atctctacgt ttcattggtt gggcaaaaag ttgaagtttt caatcttcat ctgagggaca
    18421 aatagtaaga ccccatattg tgggcaaagt tttcttttct tggtcatcat ctgcacattg
    18481 gaaaaggctt ctaggatgct ttgcccgatt ctcatattct tcacacccga tttaatcttc
    18541 ccgttctcaa tcaaaaaagt tccatctctg gttgttccag taaccatagt ttttgagggg
    18601 ttcatataat ttagatacca aaagtgagta accagtatcc ccttttcagt tgaggcgatc
    18661 agctcctctt cagtagaatc tccttcattc attactaaat tctggggata gggaccgaag
    18721 ttgtttcccg gtggcaaggc atggccagtt gactctagcc ccgcgaggtt ggcgtaatat
    18781 gaatcataca ccacgccttt acccacccca ttttcaatca aaactaccct ctttttgtcc
    18841 accccttcgt aatcaaaagg gattccggga ttttccggag aataggggtc ctcaacaata
    18901 gtaatcttat ccccggttag tttttcccca attttacctg ccataaaact tcttttttca
    18961 ataaaaccct tagctccaaa acccaaaaaa gacaaaaaca aaaggagatt agcaaccgca
    19021 gcttcttcca gaataaccgt atatttaccc gcagaaagtt caattgggtt tcttgaaagc
    19081 aatgccttct ggattgcagt ctgccccaca ttctctacgg tcaagttttt tgcgttccag
    19141 ttaaaatcct gtgcccagcc gcagttttga tctttatcgt ttacagttaa agagatatca
    19201 gttgaagctc gcttgaaata tccttttatg cccaaggagt tgaccacagc cagagcctct
    19261 tttttatctt gaaaggcgcc agcggagaaa agattatctg ccttgcattt tttgacaatt
    19321 ccttcaattc ttagggcttt gtctcgagga tcaacggaga aggtctgctc gtgaagaaga
    19381 cggttctcta ttctttggcc gggtttgggc aaagagacga aatttgggtc ttccttctgg
    19441 aactgagcta tctttatggc ttggtttatg catttttcaa tctctttttg ctccaaacga
    19501 ttggttgagg aaatgccaag tttttttccc aataccactc gcgcaaagat attatggtcg
    19561 gactgggcaa tgttttgatg aatgatattc tccgcaaaac gtgagagatt caaatcatca
    19621 gccatcaaaa ccaattcagt ctggtctgcg gaagaggata caagtgagtc ttcaaaaaga
    19681 tctaaaatct ttttttggct tgtcattctc ccacactcac tttcctaaac cgaaccggcg
    19741 aagccccctg gcttacggtc ataatttggc cgggctggcc cttgccgcaa tttggagttc
    19801 cccaaatttt ccaatagttc tcattggcaa tagcatcgca gctattccag aaccggattg
    19861 tattcccgga atagctcggg ttttttatca tttctcccaa tttgccactt tttatctccc
    19921 agccgatttc acatcccatt ttgaaattat ccctggtgtc atcaatactc cagctggcag
    19981 gagtggacat aaaaatcccg tcatcaattt ccgctatcat ctgctctagg gttttgttcc
    20041 cgggcaaaag gttagtattg gtcatcctga caataggtag attctgccag ccctgtgctc
    20101 gcatacaccc gttgctctct ttggcgattt tggcggcata ttcgcgtgaa ccaagataac
    20161 ctaccaagag gccgcctctt atgagatagc ttttatttgc cttgactcct tcatcgtcgt
    20221 atccgaaagt tcccaggccg ccgggataag tggaatcgtt gatcacattc actatctccg
    20281 aagcatactt caagttgttt aatttatctg gtgtggcaaa gctcgttccg gagaaattcc
    20341 tttctgagcc gaatactcga tctaattcca aaggatgacc tatggattca tgaatctgca
    20401 aagagagttg cgagccatcc aagattaaat ccgttattcc ctggggacaa tccttagcag
    20461 aaagcaaagc aactgcctct tgggcaattt ttgaagcgtt ctcctgcagt cccagttcct
    20521 caatcagctc ataccctttg gtctggtact gtccagagtt ttgcggatag gtgcgggtct
    20581 gcatattttt atgtccgcct tgcatggcag ttgcctctat gcccgctccg ctttgatata
    20641 tagtttgttc aagaaaggaa cctatggagc tggagaatat tttgtttgtg cggctgaagt
    20701 ccataaagga gttggttata ttggctccct ggaccttctg cataagctgg tctaaaaaca
    20761 gaagatactc tattttctct ttcaacgaaa cttcaaaagg gttctttctt atgggagtaa
    20821 gataggtatc aactattttc tctaaagggg ataagacaac atcctctttg gccagcaagc
    20881 ttgcagcttt tgccatttca attgctaatt ttgcagtatt ttttatgttc ttagcagtta
    20941 aatccggatt ggaagcaaat ccccaggagc cattagcagt gactcttatc ccaaatccga
    21001 gagaagttga gcaattgatc ggctccgggg tgccattttt tacgctcaca gtctcgtttt
    21061 ctgtttgctc gattcggata tcagcgtaac ttgcgccaaa ggatttggaa cagtccaatg
    21121 caatttgaca cgcttttttg aaatccatca taatcttatg ctatgtggca ccagatataa
    21181 aaagatggtt aaaatcactc caatcagggt aaagatagcc aggggttttg gtttcaataa
    21241 atcaagcttt ccgtctttct taaagatagt ccccttcatt aaaaacaaca taatcagcca
    21301 gtaaagtaaa atgatcaaag ttttgttatc cgtgatatcc caaccaattg gtaccccttc
    21361 ccaataacct ccaaaagtat agtgagttac ccaagcccct ataatgattc cgcctatgaa
    21421 ggtgagtgcg gcgttgaaga gtgtagccca agcgagtcga ttcagggcgt ccttattcaa
//
LOCUS       DASWLN010000116         2961 bp    DNA     linear   ENV 20-FEB-2024
DEFINITION  MAG TPA_asm: Candidatus Zixiibacteriota bacterium isolate
            SMAG_U2949 k141_1067378, whole genome shotgun sequence.
ACCESSION   DASWLN010000116 DASWLN010000000
VERSION     DASWLN010000116.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37960594
            Sequence Read Archive: SRR10376091
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Candidatus Zixiibacteriota bacterium (soil metagenome)
  ORGANISM  Candidatus Zixiibacteriota bacterium
            Bacteria.
REFERENCE   1  (bases 1 to 2961)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 2961)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (26-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/12/2024 08:28:26
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 1,440
            CDSs (total)                      :: 1,416
            Genes (coding)                    :: 1,412
            CDSs (with protein)               :: 1,412
            Genes (RNA)                       :: 24
            rRNAs                             :: 1 (16S)
            partial rRNAs                     :: 1 (16S)
            tRNAs                             :: 21
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 4
            CDSs (without protein)            :: 4
            Pseudo Genes (ambiguous residues) :: 0 of 4
            Pseudo Genes (frameshifted)       :: 0 of 4
            Pseudo Genes (incomplete)         :: 3 of 4
            Pseudo Genes (internal stop)      :: 1 of 4
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2961
                     /organism="Candidatus Zixiibacteriota bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_1067378"
                     /isolate="SMAG_U2949"
                     /isolation_source="soil"
                     /db_xref="taxon:2053527"
                     /environmental_sample
                     /country="USA: Oregon"
                     /lat_lon="42.99 N 123.63 W"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            372..1094
                     /locus_tag="VGB16_03910"
     CDS             372..1094
                     /locus_tag="VGB16_03910"
                     /inference="COORDINATES: protein motif:HMM:NF013382.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008168 - methyltransferase activity
                     [Evidence IEA];
                     GO_function: GO:0008757 - S-adenosylmethionine-dependent
                     methyltransferase activity [Evidence IEA];
                     GO_process: GO:0032259 - methylation [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="class I SAM-dependent methyltransferase"
                     /protein_id="HEX9916861.1"
                     /translation="MNDVHVMVSQKLSSKEWKWDQSIFSDEKMKERMEEYRNYLNVET
                     RNAQEIWRTEMNNYIDNFRGIVGDLATGLGGMFERLLESKAQFFPIATDVDPNVLAWT
                     SKKMKEKYEKEFVSVATDAKHFAFKDNTFDYLTSCAGLNNIPDTIVALSELHRVLKDN
                     GKIVAMQLFVEKNSNSYEMARKFNAERSLVEQFFIDDLTQVGFKKVRANIVSSAIWAR
                     NPMDGLPVAGDSQYYAIVEAEK"
     gene            1069..2700
                     /locus_tag="VGB16_03915"
     CDS             1069..2700
                     /locus_tag="VGB16_03915"
                     /EC_number="4.2.1.33"
                     /inference="COORDINATES: protein motif:HMM:TIGR02086.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="aconitase/3-isopropylmalate dehydratase large
                     subunit family protein"
                     /protein_id="HEX9916862.1"
                     /translation="MLLLRQKNKKMKTPKKKELLRLQRQYKTDKRIGEALGGIPEHLV
                     AYWRRKKGVGEYAFPKYSREKIVDLWERYADDTKAGKELGVSRAAFYKWRRKYNIREK
                     PKFLKLEQLELGLPLALLKRRSIGLQTIAQKILARKVGRSEVKVGEIVNVEPNLAMSH
                     DNAGLVIKQFKSLGVDKVWNADRIVIPLDHRAPAESEKTADAHHSIRVFVKEQGIEKF
                     YDIREGVCHQIIIEKGLVLPGELAVGTDSHTTSYGCIGALSTGIGATEMAVVWATGKI
                     WLRVPESIKMTLEGELQTGVYAKDVILYIIGKLTVEGANYKAVEFYGAGVEDMSISER
                     FTLCNLSMEMGAKFAVVPFDRVAKRYLNSVTQKSFQPVFADKDAIYENEYRFDLYRIE
                     PQVACPHNVDNVSPISKIKGIKVDQVVLGSCTNGRLDDLEIAAKILKKRKVHPDVRML
                     VIPASKDIYLKAMARGLLKTFIDAGCVILNPGCGPCLGAHQGILGKGERCIATTNRNF
                     KGRMGSVESEVYLSSPATATASAIRGEIADPREFL"
     gene            2697..>2961
                     /locus_tag="VGB16_03920"
     CDS             2697..>2961
                     /locus_tag="VGB16_03920"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_013413286.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="3-isopropylmalate dehydratase"
                     /protein_id="HEX9916863.1"
                     /translation="MIDKIKGRVWKFGDNVDTDVIYPGKYLPIIDAEEMAKHALEGMD
                     KDFPKRIKKGDIIVAGRNFGCGSSREQAATCLKFAGISCVIAKS"
BASE COUNT          920 a          542 c          764 g          735 t
ORIGIN      
        1 gtgtcattgc gaggagggcg cagcccgacg aagcaatctc ataactatca gataaacaaa
       61 gagattgcca cgctccctcc cttcgacttg gctcagggac ggtgagcaaa gtcgaaccgt
      121 acggtcgctc gcaatgacaa agagacgaaa gagcgcgttt ttaaacaacc tcaaagggta
      181 tctgtcgggt cagcctgccc tatttctcct cgctcgaccc taacggccag ggggggggac
      241 ccgacaggca cggaaaaact gataaaaaac atagaatgat tgctgtgcat gagatattgc
      301 agtgcccact ttgtagaagc gacgtcaata aggatttaga gtgcttacga tgtggtgaga
      361 ggtatgctct aatgaatgat gttcacgtta tggtaagtca gaaattgtct agcaaagaat
      421 ggaagtggga ccaaagcatc ttctcagatg agaaaatgaa agaaagaatg gaagaatata
      481 gaaactattt gaatgtggaa acaaggaatg cccaagagat ttggcggaca gagatgaaca
      541 attatattga caattttcgg ggaattgttg gcgatttggc aaccggtttg ggtggcatgt
      601 ttgaaagact acttgaatca aaagcgcaat tcttcccaat agctactgac gttgacccaa
      661 atgttctcgc ttggacaagc aagaaaatga aagagaaata tgaaaaggag ttcgtttctg
      721 tagctacaga tgcaaaacac tttgctttca aggacaacac atttgattat ttaacatctt
      781 gcgctggtct gaataatatt ccggatacaa ttgtcgcttt atctgagctt cacagggttt
      841 taaaagataa tggcaaaata gtggccatgc agctttttgt tgaaaagaat agcaattcat
      901 atgaaatggc gaggaaattc aatgcagaaa ggtctcttgt tgaacaattc ttcattgacg
      961 atttgaccca ggtcggtttc aagaaggtac gagcaaacat agtgtcttct gcaatttggg
     1021 cgagaaaccc aatggatgga ctcccggtcg ctggtgattc gcagtattat gctattgttg
     1081 aggcagaaaa ataagaaaat gaaaactccc aagaaaaaag aactgctacg gcttcaaaga
     1141 caatacaaga ccgacaaaag aatcggtgag gcactgggtg gaatacccga gcatttggtt
     1201 gcctactgga gaaggaagaa gggagtaggg gaatatgctt tccccaagta cagtcgcgag
     1261 aagattgtgg atttatggga acgttatgct gatgatacca aagcaggcaa ggaactcgga
     1321 gtttccagag ccgcctttta caaatggaga agaaaataca atattcgcga aaagcccaag
     1381 ttcttaaaat tggagcagtt ggaattggga cttcctcttg ccctcttaaa aagaagaagt
     1441 attggtttac agaccattgc ccagaagata ttagctcgaa aagtcggtcg gtccgaagtc
     1501 aaagtcggcg agatcgtaaa tgtcgagcca aatttggcaa tgagccacga taatgccggc
     1561 ttggtgataa agcagtttaa aagcttagga gttgataaag tctggaacgc ggatagaatc
     1621 gtaattcctt tagatcacag agccccagcg gaaagcgaga agaccgcaga tgcccatcat
     1681 tccataaggg tttttgtcaa agagcagggg atagaaaagt tttatgatat tcgggaaggg
     1741 gtttgtcacc aaatcataat cgagaaaggg ttagtgcttc caggtgagtt agcagttgga
     1801 accgattcgc atactacttc ctatggatgc ataggtgccc tctccaccgg cattggtgca
     1861 actgagatgg cggttgtctg ggcaactgga aagatttggc tgagagttcc ggaatcaata
     1921 aaaatgactt tggaagggga gcttcagacc ggagtctatg ctaaggatgt gattttatat
     1981 ataattggca aattaactgt ggagggagca aattataaag cagtggaatt ctacggtgcg
     2041 ggagtagaag atatgtcaat ctccgagcga ttcaccctat gtaatctctc tatggagatg
     2101 ggagccaaat ttgcagttgt gccttttgat agagtagcta aaagatattt aaatagcgta
     2161 acccaaaaga gcttccagcc ggtatttgca gataaagacg caatttatga aaatgaatat
     2221 cgcttcgacc tttaccgcat cgagccgcaa gttgcctgcc cgcataatgt ggacaatgtc
     2281 agtccgataa gtaaaatcaa agggataaaa gttgaccagg ttgttctggg ctcctgcacc
     2341 aacggtcgtt tggatgattt ggagattgcc gccaagatat tgaagaagag aaaagttcat
     2401 ccggatgtaa ggatgctggt catacctgct tcaaaggata tttatcttaa agctatggct
     2461 cgcggattat taaaaacatt tatcgatgcc ggttgcgtta tactcaatcc tgggtgtggg
     2521 ccctgcctgg gagcacatca aggtatcttg ggcaaagggg agaggtgtat cgccacaacc
     2581 aatcgcaatt tcaaaggcag gatgggttca gttgaatccg aagtctattt atccagccca
     2641 gccactgcca ctgcctcagc tatcagggga gagatcgctg acccgaggga gtttttatga
     2701 ttgataaaat caaaggaagg gtttggaaat ttggagataa cgttgatacg gacgttatat
     2761 atccgggaaa atatcttccc ataatcgatg cggaggagat ggcaaagcat gctttggaag
     2821 ggatggataa ggattttccc aagaggataa agaagggtga tataattgta gcgggaagga
     2881 attttggatg cggcagttcc agagagcaag cagcaacttg ccttaaattc gctggcataa
     2941 gttgtgtcat tgccaaatcc t
//
LOCUS       DASWLN010000117         6517 bp    DNA     linear   ENV 20-FEB-2024
DEFINITION  MAG TPA_asm: Candidatus Zixiibacteriota bacterium isolate
            SMAG_U2949 k141_482361, whole genome shotgun sequence.
ACCESSION   DASWLN010000117 DASWLN010000000
VERSION     DASWLN010000117.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37960594
            Sequence Read Archive: SRR10376091
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Candidatus Zixiibacteriota bacterium (soil metagenome)
  ORGANISM  Candidatus Zixiibacteriota bacterium
            Bacteria.
REFERENCE   1  (bases 1 to 6517)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 6517)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (26-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/12/2024 08:28:26
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 1,440
            CDSs (total)                      :: 1,416
            Genes (coding)                    :: 1,412
            CDSs (with protein)               :: 1,412
            Genes (RNA)                       :: 24
            rRNAs                             :: 1 (16S)
            partial rRNAs                     :: 1 (16S)
            tRNAs                             :: 21
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 4
            CDSs (without protein)            :: 4
            Pseudo Genes (ambiguous residues) :: 0 of 4
            Pseudo Genes (frameshifted)       :: 0 of 4
            Pseudo Genes (incomplete)         :: 3 of 4
            Pseudo Genes (internal stop)      :: 1 of 4
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..6517
                     /organism="Candidatus Zixiibacteriota bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_482361"
                     /isolate="SMAG_U2949"
                     /isolation_source="soil"
                     /db_xref="taxon:2053527"
                     /environmental_sample
                     /country="USA: Oregon"
                     /lat_lon="42.99 N 123.63 W"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            201..2144
                     /locus_tag="VGB16_03925"
     CDS             201..2144
                     /locus_tag="VGB16_03925"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEX9916864.1"
                     /translation="MSKLRITVTAGLVGLAFLVGLMLLSCDAGRKGKERQNRPPTVKF
                     SNVPPDSAAFSFNPEVSWYALDIDGYVIKYQYAVIIGDSLHVVGKLWNGGGTLTAADS
                     ACSVLVRIPPEAWVDSLRHIDSTIVPDNIDKFISAVDTTVSIVKVQLFARENPNDTIT
                     QHLFVRAIDNDNAVSNVIHRMFSRNNHKPKASISYIPFRSGTDVKSVYCLPETTQTWK
                     GIKLTWDGSDSDDYKGTQPDFVFKWELWGPFADSFAVINNKTEYDQLRSSSDSVFLVS
                     SSFNETDSNSLVEDKSLLLTGLVNSPHLVRNIDTVQYPDLGYGWYLFIAWTMDDAFVF
                     SDPFTSSNPDGHLWFKIVHPQFTYQSDPSHSSNPVRKILVYDYGNYGRGDTGGEKDSV
                     NVRLFYQRAVGALMDSLCNTLTFRKHKYQDLAYFSNDTLSRYDLVIYLNEGKPQDVIG
                     VLQYKEYLKVGGKVWAIGNGPETFGVASASKGLNFFEDVTSSLVPYADYAMTYFGLLG
                     VYDAGWSTSGADRNEEFIGANPYGSALDLPVLKTDTIKVRDQINWSTGAPDSTGKLAH
                     TSYTYLGKATRIYTFVSAFPATSVLHFRPCGSIYEGPLGPDGHPIYKTAHLSFGLHFI
                     DEGVGGATRDSLFSEIVRWFWED"
     gene            complement(2212..4548)
                     /locus_tag="VGB16_03930"
     CDS             complement(2212..4548)
                     /locus_tag="VGB16_03930"
                     /inference="COORDINATES: protein motif:HMM:NF024629.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="tetratricopeptide repeat protein"
                     /protein_id="HEX9916865.1"
                     /translation="MNKEAGPQQKKEFLIFVFIFIFSFALRLIYLLQIKDNPYFASPT
                     MDPLYHDIWAQDIANGNWVGNKAFFRAPFYAYFLALIYKVFGHNYFIPRLIQHIIGSF
                     SCVLVYILAKKIFNRKIAILSAILASTYWIFIYFEGELLLDSLLVFFDLLLIILLLDA
                     SQNPNLKTHLKTHLKTQNSGNSRWKRWITCGLVLGLSAITRPNILIFIPVVWLWTILL
                     FVKSKKFKVVFGHIVFFTLGTMLVILPVTLRNYVVGKDFVLIASQGGVNFYIGNNPDA
                     DGMSATFFHQGDWQSSDFEYIAQAAMDKELKPSQISDFYYKKGLEFIFDQPASAFRLF
                     IKKIYMFWNSFEVSNNQNVYFFKRYSTLIQILPIGFWLVGPLSLVGMGLSFGFKKNTL
                     LPMLFLFSYMLTVISFFITDRYRLPVLPILIIFSSYAIFWLIEKIQRKDFSRIALFAL
                     IVIPLFWLVNSNLYHLNKKDFYQSYFSLGNTYLKQGDTRKARDMYEQALIQNPLAPKV
                     HLNLGMIYFKERDWGRAEEELLKELKVNPKEEKVYNNLSVIYRLKRDYDKAIVAAQKA
                     LEIKPYFKEACINLSLAYKEKGGLTKARETLYQGLEIVPGFTQADFLLAYLYQQENAS
                     DSALNYYQKVIEETSVKKDVLYNLENLFLEEPYASPEKTKAYAYFNMGLIWARRGQLD
                     GAEELFLEAIKLKTNFASAYSNLGNIYEIKGESGKAIENFRRAIEYDPKNPIYYYNLG
                     VSYQRIGEMTQAIQMFETALSLDPGFGPAKEKLSKLPN"
     gene            5201..>6517
                     /locus_tag="VGB16_03935"
     CDS             5201..>6517
                     /locus_tag="VGB16_03935"
                     /inference="COORDINATES: protein motif:HMM:NF012311.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008236 - serine-type peptidase activity
                     [Evidence IEA];
                     GO_process: GO:0006508 - proteolysis [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="S8 family serine peptidase"
                     /protein_id="HEX9916866.1"
                     /translation="MHEFCPSTLLRTVSLSNRVLRLVKRSLFFLIISILVCGCQLWAG
                     EINPDLHFKLRNLRPSEYHSCLVVLKERAKTEELNLELGQKRANRITRHQVILNSLRT
                     KASQTQVNLIGYLKDRIKENFVKDFKSFWIANIIQVTATRDEIEKIALMPEVEKIYDD
                     VQIELIQPISCEDNSSSESSNKDNFDAIGVRKVWNMGFTGKGRLVCNFDSGVEGTHPA
                     LSSKWRGNNGGTSSSSWFDPYGSSSPIDSRGHGTHTMGIMVGSTPGDTLGVAFGAQWI
                     SAGVVDRGVGVSQTISDILSAFQWAVDPDGDIQTAEDVPDVINNSWGVPLGVLPSCDQ
                     VFWEAIDNVERAGIVTIFSAGNEGPNSFTIRNPADRISSPYNSLAVGAVNAHADSLPV
                     ATFSSRGPSGCDGQSIKPELSAPGVEVRSCYKDGQYKVLSGTSMAAP"
BASE COUNT         1919 a         1286 c         1505 g         1807 t
ORIGIN      
        1 gccaatgata cgggaggctt ctgtgcaggt tcaggcctga agatagacct atccggcctc
       61 aagttgggct ttgactacgc ttaccatgat tttggacatt tagatcaaat ccatagactc
      121 tcagccagcg tgaagtttta aaggagagga agaaaaatca cattaatctc atatatgaat
      181 ctcaaaggag gttttagtcc atgtctaagc tcaggataac agtaacagct ggattggtag
      241 gattagcttt tctggtaggg ttaatgctac tcagttgtga tgcaggcaga aagggaaaag
      301 agcgacaaaa caggccacca acggtaaagt tttccaatgt tcctcctgat tcggctgcct
      361 tctcctttaa tccggaagta agctggtatg ccttagatat agatggttat gtcataaaat
      421 accagtatgc agtgataatt ggagattcac ttcatgtagt tggcaagttg tggaacggtg
      481 ggggaacgtt aactgcagcg gacagcgctt gcagtgtttt agttagaatt cctcctgagg
      541 cttgggttga ctctttacga catatagaca gtacaatagt gccagataat atagataaat
      601 ttatctcggc tgtggatacc acggttagca ttgttaaagt tcagcttttt gccagagaaa
      661 acccaaatga taccataact cagcatcttt tcgtcagggc aatagacaat gataatgcag
      721 tctccaatgt aatccatcga atgtttagcc gcaataatca caagcccaag gcaagcatca
      781 gttatattcc attccgcagc ggtactgacg taaaaagcgt atattgtctg cccgagacta
      841 cccagacctg gaagggaata aagttgacct gggatggttc agattcagat gattataaag
      901 gaacacagcc cgacttcgta ttcaaatggg agctctgggg accttttgct gatagtttcg
      961 cagttatcaa caataaaact gaatatgatc aactgcgtag tagtagcgac tcagtttttc
     1021 tggtgtcttc ttccttcaat gagactgaca gtaactcttt ggtcgaagat aaaagcttgc
     1081 ttttaaccgg actggttaac tctcctcatc tagttcgcaa tatagatacc gttcagtatc
     1141 cggacttagg atatggctgg tatcttttca tagcctggac tatggatgac gcttttgttt
     1201 tctccgaccc ttttacctcg agcaatccag atggccatct ctggttcaag atagttcatc
     1261 cacagttcac ctaccagtca gacccgtcac atagctcaaa tccggtgagg aagatattgg
     1321 tttatgatta cgggaattat ggcagaggtg atacgggtgg tgagaaggat tcggttaacg
     1381 tgaggctttt ctaccaaagg gcagttggag ctctgatgga ttcgctgtgc aacactctga
     1441 cttttagaaa gcacaagtac caagacctgg catattttag caacgatacg ttgtcaagat
     1501 atgacttggt aatctacctt aatgagggta aaccccaaga tgtaataggg gttctgcagt
     1561 acaaggaata cctgaaagtg gggggcaagg tgtgggcgat agggaacggt cccgagactt
     1621 ttggcgtggc ttcagcatcg aaaggcctga acttttttga agatgttact tcaagcttgg
     1681 tgccttatgc cgattacgcc atgacgtatt ttggcttact cggcgtatac gatgctggct
     1741 ggtctacatc tggagctgac cgcaatgaag agttcatcgg agcaaaccca tatggtagtg
     1801 ctctggacct gccggtgtta aaaacggata cgatcaaagt aagggatcaa ataaactggt
     1861 cgaccggcgc accggattca acaggaaaac ttgcgcatac ctcttacaca tacttgggga
     1921 aagccacaag gatatatact tttgtctcag cttttccagc aacttcggtt ttgcatttca
     1981 ggccttgtgg gtccatttac gagggtccgc ttggtccgga tgggcacccc atttacaaaa
     2041 cagctcatct aagtttcggg ttgcatttta tagatgaggg tgtggggggc gcaacgagag
     2101 atagcctgtt cagcgagata gtcagatggt tctgggagga ttagaatctg attcatagat
     2161 aataaagggg tcggcttaga gccgacccct ttattgttcg taggaaaatc cttagtttgg
     2221 caacttgctt agcttctcct ttgctggacc gaatcccgga tccaggctca atgccgtttc
     2281 aaacatctga atggcttggg tcatctctcc aattctctgg tatgatactc ctaggttata
     2341 ataataaatc gggtttttgg ggtcgtattc gattgctctt ctgaaatttt cgattgcttt
     2401 accggattcc cccttaatct catatatatt tcccagattg gaataagccg aggcaaaatt
     2461 agtttttagc ttaattgctt caaggaacag ctcttcagcc ccatctaatt gtcctcttct
     2521 tgcccaaatc aatcccatat tgaaataggc gtaagccttg gtcttttccg gggaggcgta
     2581 aggctcttcc aaaaacagat tctccagatt gtagagaaca tctttcttaa ccgaagtttc
     2641 ctctattacc ttctggtaat aatttaaggc ggaatcagaa gcattctcct gctggtaaag
     2701 ataggctaaa agaaaatcag cttgagtaaa tccgggcaca atttccagcc cttgatataa
     2761 ggtctctctt gcttttgtca gtcccccttt ttccttataa gctaaagata ggtttataca
     2821 agcttcttta aagtagggct tgatttccaa agccttctga gctgcgacta tggctttatc
     2881 gtagtctctt ttgagtcgat agataactga aagattatta tacacttttt cttctttggg
     2941 gtttactttt agttccttca aaagctcttc ttctgccctg ccccaatctc tctccttaaa
     3001 gtagatcatc cccagattca gatgaacctt gggagccagt ggattttgga tcagagcttg
     3061 ttcatacata tcccttgctt tccttgtatc tccctgtttg agatatgtgt ttcccaagct
     3121 gaaatacgat tgataaaaat cctttttgtt caggtgataa aggttggagt taaccagcca
     3181 gaaaagggga ataacgatta gagcgaatag ggcaattctg gaaaaatcct tacgttggat
     3241 tttttcgatt agccagaaaa tagcataaga ggagaagatt atcagtatag gaagaacagg
     3301 cagtctgtat cggtcagtta tgaaaaaact tatcacagta agcatatagc tgaacaagaa
     3361 aagcatgggc agcaaagtgt tctttttgaa accaaaagaa agccccatac ccacaagcga
     3421 cagaggacct accaaccaaa aacctatggg cagaatctga attaaagtgg aatacctttt
     3481 gaaaaaatag acattctgat tgttacttac ttcgaaggag ttccagaaca tatagatttt
     3541 cttaatgaat agcctgaagg cagaagctgg ctggtcaaat ataaactcca gtcctttttt
     3601 gtaatagaaa tcggatattt gcgaaggctt cagctcctta tccatagcag cttgggcgat
     3661 gtattcaaaa tcacttgatt gccagtcacc ttgatggaaa aaggtagcac tcatcccgtc
     3721 cgcatccgga ttattaccta tataaaaatt gactccaccc tgtgaagcga tcaaaacaaa
     3781 atcctttccc acaacataat ttctgagagt aaccggcaag ataacaagca tcgttcctag
     3841 agtgaaaaag acgatgtgcc cgaaaacaac cttaaatttt ttacttttca cgaatagcaa
     3901 aatcgtccat agccagacta ctggaatgaa aatcagaatg tttggtcggg tgatggcgga
     3961 taagccgaga actaaaccac aggttatcca tcttttccag cgcgaattcc ccgaattctg
     4021 ggtcttgaga tgggtcttga gatgtgtctt gagattggga ttctgagaag catcgagcag
     4081 gagtataatt aaaagcaaat caaagaaaac taaaagagaa tccaacagaa gctcgccttc
     4141 aaagtatatg aaaatccagt aagtcgaagc caaaatggcg gagagaatag ctatttttct
     4201 attgaatatt tttttggcca gaatgtaaac tagaacacag gagaaggagc cgattatgtg
     4261 ctgaatcagt cgagggataa aatagttgtg cccgaagacc ttgtatatca aagccaaaaa
     4321 gtaagcatag aaaggtgctc gaaagaaagc cttatttcca acccaatttc cattggctat
     4381 atcctgtgcc cagatatcgt gatagagtgg gtccatagtg ggagaggcaa agtaaggatt
     4441 atcctttatc tggagaagat agatgagcct cagagcaaaa ctaaaaatga agatgaagac
     4501 aaatatgaga aactcttttt tttgttgggg accggcttct ttgttcacag gttcaatata
     4561 tttataggtt taaaccttgt caaacacaaa aaagacagca ctatccccaa aaacaagaca
     4621 tagaaagagg gaagtgtttg tccgaattct ggatcttgag acccgaagtc tgggtcttga
     4681 gatgagataa ggagaattta aagaatagga aagcaggaat acaatttcct tatctttagc
     4741 aaagaaatac tcttctctat cctctccctt gaagggagag gataaaaggt gagggtgagg
     4801 agtattacag ctgtgaggct gtgggggggt atttcttttc ccccttactc catctctccc
     4861 ctaaggggag agagaaaccg gaggaaatag ccattgagac attctgatat gtcttgaaaa
     4921 aaagtgggga tgctcctgca aaacagacta tgtgaaactg gtttcatata tatcaaagat
     4981 ttttatgcaa ttcgttgcac agaaaagagt caggtttttc tgaggggcca agtcccgtga
     5041 aatgtagata aaatttgctc acccgtaaga ttatgttaca ccataactta gggttgacta
     5101 ccaactcagt aagaatgtag cattacggtt gataagcata gcctaacttg ggcacttggt
     5161 ttgctttaag aggaggtgaa attccaagga ggcataaatt ttgcacgaat tctgtccttc
     5221 gaccttgctc aggacggtga gcttgtcgaa ccgtgtcttg agattagtta aaagaagctt
     5281 atttttctta ataatatcaa ttctggtttg cggctgccag ctttgggcag gtgaaataaa
     5341 tcctgacttg catttcaagt taagaaatct tcgcccaagc gaatatcatt cctgcctggt
     5401 agtattgaaa gaaagagcaa aaacagagga attgaatctc gagttaggtc aaaagagagc
     5461 aaatcgcata accagacatc aggtcatatt aaattcgcta cgaaccaaag ccagtcaaac
     5521 ccaggtgaat ctgattggtt acttgaaaga taggataaag gaaaacttcg taaaagattt
     5581 taagagtttc tggattgcaa atatcattca agtaacagca accagagacg agatagagaa
     5641 aattgctctt atgccagagg ttgaaaagat atatgacgat gtccaaatag aattaatcca
     5701 gccgataagt tgcgaggata attcttcttc cgaaagcagc aacaaagata acttcgatgc
     5761 tattggcgta agaaaagtgt ggaatatggg tttcaccgga aaagggagat tggtctgtaa
     5821 tttcgacagc ggggtggaag gaacacatcc agccttgagc tccaagtgga gggggaataa
     5881 tggcgggaca tcaagttcat cctggtttga cccttatggc tcttcttcac cgattgattc
     5941 tcgcggacat ggcacccaca ctatgggaat aatggttggt tcgactccag gtgataccct
     6001 gggggtggct ttcggtgccc aatggatttc cgcaggagtg gtggatagag gagtgggtgt
     6061 atcgcaaacc ataagtgata tcctatccgc ttttcaatgg gcagttgacc cggatggaga
     6121 tatccaaacc gcagaagacg taccagatgt aatcaataat tcctggggag ttcctctggg
     6181 ggttttgccg agctgtgacc aggttttctg ggaggcgata gataacgtgg agcgggctgg
     6241 aatcgtgacc atcttttcag ctgggaatga gggacccaat tcttttacca tcaggaaccc
     6301 ggcagacaga ataagttcac cttataatag tctggctgtg ggagcagtta atgcacatgc
     6361 tgatagctta ccagttgcca ctttcagcag tcgcggtcct tcaggatgtg atggtcaaag
     6421 cataaaacct gaactttccg ctccgggagt ggaggttcgt tcctgttata aggacggaca
     6481 atataaggta ctaagcggta cctctatggc agcccct
//
LOCUS       DASWLN010000118         2948 bp    DNA     linear   ENV 20-FEB-2024
DEFINITION  MAG TPA_asm: Candidatus Zixiibacteriota bacterium isolate
            SMAG_U2949 k141_990379, whole genome shotgun sequence.
ACCESSION   DASWLN010000118 DASWLN010000000
VERSION     DASWLN010000118.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37960594
            Sequence Read Archive: SRR10376091
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Candidatus Zixiibacteriota bacterium (soil metagenome)
  ORGANISM  Candidatus Zixiibacteriota bacterium
            Bacteria.
REFERENCE   1  (bases 1 to 2948)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 2948)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (26-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/12/2024 08:28:26
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 1,440
            CDSs (total)                      :: 1,416
            Genes (coding)                    :: 1,412
            CDSs (with protein)               :: 1,412
            Genes (RNA)                       :: 24
            rRNAs                             :: 1 (16S)
            partial rRNAs                     :: 1 (16S)
            tRNAs                             :: 21
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 4
            CDSs (without protein)            :: 4
            Pseudo Genes (ambiguous residues) :: 0 of 4
            Pseudo Genes (frameshifted)       :: 0 of 4
            Pseudo Genes (incomplete)         :: 3 of 4
            Pseudo Genes (internal stop)      :: 1 of 4
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2948
                     /organism="Candidatus Zixiibacteriota bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_990379"
                     /isolate="SMAG_U2949"
                     /isolation_source="soil"
                     /db_xref="taxon:2053527"
                     /environmental_sample
                     /country="USA: Oregon"
                     /lat_lon="42.99 N 123.63 W"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            complement(187..786)
                     /locus_tag="VGB16_03940"
     CDS             complement(187..786)
                     /locus_tag="VGB16_03940"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003350590.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="transglycosylase SLT domain-containing protein"
                     /protein_id="HEX9916867.1"
                     /translation="MSRSAVILIIILLIIAFVLLPYGVKSEQLTGLVTNSLYTDHKAH
                     QVGDLLTVLIYESAQGSNVSESNNKRDNKFEATGTEGAGVLKFIPLKAVIYHESGGNP
                     QAVSPRGAKGLMQLADSTAQEIRVKDIFDPRENILAGTKYLKSLIQRFGGDLKLALAA
                     YNAGPSIVDKIRAVPPFKETISFVNKVLEFVQRQTQLKS"
     gene            complement(1888..>2948)
                     /locus_tag="VGB16_03945"
     CDS             complement(1888..>2948)
                     /locus_tag="VGB16_03945"
                     /inference="COORDINATES: protein motif:HMM:NF018449.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=3
                     /transl_table=11
                     /product="SBBP repeat-containing protein"
                     /protein_id="HEX9916868.1"
                     /translation="STADNGTNRDYATVKYDSRGKQLWVKRYNGPENKLDEPSAIAID
                     RFGNAYVTGKSMNKGIDRDYATIKYYPNGDTAWIRRHQGPTLDEASAIAVDGFGNVYV
                     TGESYDARTNWDYLTIKYDSSGNELWTRRYNGTGNRSDSPSAIAVDSSGNIYVTGTSQ
                     GPLKKHEEEYIDKGDYLTIKYDSNGKQLWVKRYNGPEDSYDGACAMAVDNFGNVYVTG
                     ESEGGWESLTDYLTIKYSSSGKQLWIRKFGGPRWDRASDIAIDDSGNAYVTGEHGISE
                     ARQYIATIKYDRNGKQLWKEIYNYPGKRYIMPSAITVDGSGNIYVTGGVEESIEDRLH
                     HKEVLFDYITIKYFKAKR"
BASE COUNT          768 a          691 c          551 g          938 t
ORIGIN      
        1 ccaatttttc cgcctcgagt atggccttct cctgctcacc cacagagtcc tgcagggact
       61 ggatgttgtt ttcagccaaa agctgctgct ctttggtcaa aagggtcatg aagttctcca
      121 agcagacaac ttccccattt atgacctcta tcaatctgtt taccttgcgt tgcaatttat
      181 cctccatcat gatttcagct gagtttgtct ttggacaaat tccagcacct tattgacgaa
      241 actaattgtc tccttgaaag gaggaactgc tctgattttg tcgactatgc ttggcccggc
      301 attataggct gccaaagcta atttcaagtc accaccgaag cgctggatta ggctcttcaa
      361 gtattttgtc ccagctaaga tattctctct ggggtcaaaa atgtccttca cccttatctc
      421 ctgtgcagtc gaatcagcca actgcattaa tcctttagct cctctgggag agactgcttg
      481 aggattacca ccggattcgt gatatattac cgccttgagg gggataaatt tcagtacccc
      541 tgctccttca gtcccggtgg cttcaaattt gttatccctc ttattgttac tctccgagac
      601 attgcttcct tgtgcgctct catagattaa tacagtaagc aggtcaccca cctgatgagc
      661 tttatggtca gtataaagtg aattagtgac caagccggtc aactgttcgg atttcactcc
      721 atagggaagc aaaacgaagg caattatcaa aaggatgatg attaagatta cggctgaacg
      781 agacatttct cctccttttt atcatttcta atcacctatt tttgccaaaa tggaaacagc
      841 atggtttgct ccggattttg aattcagcaa cttctatgcc aatagagact taatttcaga
      901 ggagaattaa ggggaatcta ttgaaaatgg gacgatgagg ttaagttatt ggtatataat
      961 ggcttaaaac tacaacttgc tttattcttt ttccgaattc ttaactatcc tttagaacca
     1021 attttatatc ctgattttgc ctacccaaaa aacgaacaat tttccttatt ggaattgaac
     1081 ttttttcctt atcaattttc attcatacag tatgaactta tctggacttt cagcctgttt
     1141 cccgcatttc ttgttcttga ggctgtctaa aaactatgaa aatgtcgttt atgtcattct
     1201 gaacccgttc gcttcgctca gggcaggctc cgctgttgtc attctgaatg gagcgttagc
     1261 ggagtgaaga atctgggtgg aatgggaggc aagcgagatt cttcgtcccg aaagctttcg
     1321 ggactcagaa tgacattcct agacagtctc taaaggagag ggtgaaggta tgttccgcgt
     1381 ggttattctt tttcgtaggt gtggaaattt ccttcgacct tgctcaagat ggtgaagacg
     1441 ttcggctgag cggttcgacc gtcctgagcg gctcaccgtc gaaggactca gtcgaagccc
     1501 ttgttgaacc atatcccaca ccgtctatga atgaacaagg ggtaggtaat ggctgcgcca
     1561 ttacctttgg ggaacggtgc aaccgttccc tgcccgctgt acgtacaaac caaaaggatc
     1621 tatccgtttt atcgtaggcg acattcgcga atgtcgccta caactgagaa taaacaaagg
     1681 acagacacag aggtctgtcc ttaccaaaac aaagctataa cctgccttat tattcctcct
     1741 cagataagta atccagccgg gcaagttaat aatctaacgt agaaactgta agaatagcaa
     1801 aagtcgccaa tgaatcgcaa attactaaaa agctctggag cttttctcca gagcctaaat
     1861 tctactcttt tggtttatgt caaaaaacta tctcttagct ttaaaatatt ttatggtaat
     1921 gtaatcaaaa agcacttctt tgtggtgtaa cctatcttca atactttctt ccactcctcc
     1981 tgttacatag atattaccag aaccatctac agttatggca gagggcatga tatatctctt
     2041 ccccggatag ttgtatatct ctttccagag ctgttttcca tttcgatcat actttatagt
     2101 agctatatat tgtctcgctt cactgatacc gtgctcgcca gtcacatacg cattgccaga
     2161 atcatctatg gctatgtcag aagctctatc ccatctcggt ccaccaaact ttcttatcca
     2221 aagttgtttt cctgaggaac tatactttat agtaagataa tctgttaaag actcccaccc
     2281 accttcactt tctcctgtta catacacatt gccaaaatta tccacagcca tggcacatgc
     2341 tccatcgtag ctatcctctg gtccgttata tctttttacc caaagctgtt tcccatttga
     2401 gtcatatttt atggttagat aatcgccttt gtcgatatat tcttcctcat gttttttgag
     2461 agggccttgg cttgttcccg ttacataaat attaccagaa ctatctaccg ctatggcaga
     2521 aggtgagtca gaccgattcc cagtcccatt atatcttctt gtccaaagtt cattacccga
     2581 agagtcatat tttatggtaa gataatccca atttgtccga gcatcgtagc tttctccagt
     2641 aacataaaca ttaccaaaac catccactgc tatagcagaa gcttcgtcga gagtaggacc
     2701 ctgatgtctt cttatccaag cggtatctcc atttgggtaa tactttatcg tagcatagtc
     2761 gcgatctatc cctttattca tactttttcc tgtcacatag gcattaccga atctatctat
     2821 tgctatggca gaaggttcgt ctaatttgtt ttccggtcca ttatacctct taacccaaag
     2881 ctgtttccct cttgaatcat actttacagt agcgtaatca cgattcgttc cgttgtcggc
     2941 ggtactta
//
LOCUS       DASWLN010000119         3724 bp    DNA     linear   ENV 20-FEB-2024
DEFINITION  MAG TPA_asm: Candidatus Zixiibacteriota bacterium isolate
            SMAG_U2949 k141_611176, whole genome shotgun sequence.
ACCESSION   DASWLN010000119 DASWLN010000000
VERSION     DASWLN010000119.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37960594
            Sequence Read Archive: SRR10376091
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Candidatus Zixiibacteriota bacterium (soil metagenome)
  ORGANISM  Candidatus Zixiibacteriota bacterium
            Bacteria.
REFERENCE   1  (bases 1 to 3724)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 3724)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (26-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/12/2024 08:28:26
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 1,440
            CDSs (total)                      :: 1,416
            Genes (coding)                    :: 1,412
            CDSs (with protein)               :: 1,412
            Genes (RNA)                       :: 24
            rRNAs                             :: 1 (16S)
            partial rRNAs                     :: 1 (16S)
            tRNAs                             :: 21
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 4
            CDSs (without protein)            :: 4
            Pseudo Genes (ambiguous residues) :: 0 of 4
            Pseudo Genes (frameshifted)       :: 0 of 4
            Pseudo Genes (incomplete)         :: 3 of 4
            Pseudo Genes (internal stop)      :: 1 of 4
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..3724
                     /organism="Candidatus Zixiibacteriota bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_611176"
                     /isolate="SMAG_U2949"
                     /isolation_source="soil"
                     /db_xref="taxon:2053527"
                     /environmental_sample
                     /country="USA: Oregon"
                     /lat_lon="42.99 N 123.63 W"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            <1..2199
                     /locus_tag="VGB16_03950"
     CDS             <1..2199
                     /locus_tag="VGB16_03950"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEX9916869.1"
                     /translation="PLADVGKKQVEVPKHIASKGPEAIREYLEAKQPKDEPSPSETPE
                     EEPNVPIPKDETTAAETEEKPQAPSLKEKSVDERVQEQKQAIIDTKKASREHYELEDK
                     ENDRTYKISKITEKEVRGKTVHKFYYEKDRYLSGANIDAILRGNINEEEKKAAKAAKE
                     AGKAEKNAARKAADTKRTEQKERYEAGKTRLGEGLEESVENQREFLRAGHDIGYELRR
                     GKEALTFEKYDPEVSNPFVFRDPNGNRQYLHENDVDRLIKRRFEKENKEIVAELKPES
                     RAKEITGEGLETAGKGAKALIETAEKNPVGRFLMGRAKAYLEHINPKDKEQPYYWTGF
                     AFGFGSNIALSLFAPVLPMLTKPLRSAIFSGGVFAISRGVNLFLTRNLEKIGNIIGAD
                     NNFVNDWLKTLNKARQLEAEGKIEDSIELRNQQSKDSFLKAGYKERSDYWAWTQDIGR
                     AAMIARIEQEVAKYTGFKQKLNHISAGFAAGNLGATGFLGLENLFSEAFKKMPHLGLF
                     GGGDEAQIGAPEGAGPEVPHHAPTPGAPEHTPTPGVPEHTPTPTPGEIPEGPGVEPTP
                     GVEPGEVPTEPGEVPIEPGGEPIPVPIPPEAPPMAIEDIITNPEYSSAFEIPDGATPG
                     QVFLNNGFEDATLWEANEANAHLFGAHVEANYDMLEEMSRNVAASGIPVEDFPASAEI
                     PGLIEAASAGDAEAMHRLTEALHYWPAGDDFNLLTPEGVQVVNKALAARI"
     gene            2206..2526
                     /locus_tag="VGB16_03955"
     CDS             2206..2526
                     /locus_tag="VGB16_03955"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEX9916870.1"
                     /translation="MADLEKVINILKENKVEDKVIATFIEGLNNLLAQKIQVELISVL
                     DNEKDIARLNELPQDQMTEELAKVYKAKAGKDIAIVSQEILDGFVTGFLTQYHKQKLE
                     EKKS"
     gene            complement(2624..>3724)
                     /locus_tag="VGB16_03960"
     CDS             complement(2624..>3724)
                     /locus_tag="VGB16_03960"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEX9916871.1"
                     /translation="RFEQKKAEQEKKDQSQIEVVLEKIRGFLTRSKKEAWETMGERDE
                     LATVVKRLEGKLAPDQTGFTLEVFVGSPRTEVSQGPSEKVVVMTDNMEQAKGIHATLG
                     KEQQVIVASPHQLPFKESALERIVDPHKLTHVGEKSRESLGTPLQSLENLYAEIKAFI
                     LEKKGEVGYLAARPTYVTEGTHEVWKAKLGESLRERASVMAQNGKLVFSVGVIPPQWE
                     TESGYMAHTTQVGEVFLKPDEIKDLVAKAGLKIDTAYAGMGPRSQMIHTENAVKALIM
                     STTGWGFERFADEVLIPIAHSAVNMLGTGKRESFEGLAPEKIREKMKLEKVDLDHFEE
                     ARTSGLASARTPEGKPLPPDRVIIVASKETGD"
BASE COUNT         1123 a          875 c          807 g          919 t
ORIGIN      
        1 cctctcgcgg atgttggaaa aaaacaagta gaagtcccaa agcatattgc tagtaaaggc
       61 ccagaagcga taagggagta tttagaagcg aaacagccga aagatgagcc ctctccctcc
      121 gaaactcctg aagaggaacc aaacgtccca atacccaagg atgagaccac agctgcagaa
      181 actgaagaaa aaccacaagc accctccctc aaagaaaaat cggttgatga gcgtgtccaa
      241 gagcaaaagc aagccatcat cgacacaaag aaagcttcca gagagcatta cgagcttgag
      301 gacaaggaga acgacagaac ctacaaaata tccaagataa ccgaaaaaga agtccgtggt
      361 aaaacagtac acaagtttta ctatgagaag gaccgctatc ttagtggcgc caatatagac
      421 gcaattctcc gcggtaatat aaatgaggaa gaaaagaaag ctgcgaaggc agcaaaagag
      481 gcgggaaaag ccgaaaagaa tgccgccaga aaagcggccg ataccaaaag aaccgagcaa
      541 aaagaaaggt acgaagcggg aaaaacccga cttggagaag gtctcgaaga atccgttgaa
      601 aatcagcggg agtttctccg ggctggtcat gatattggat atgaacttcg acgcggaaag
      661 gaagccttaa catttgaaaa gtatgatccc gaggtaagta atccctttgt atttagggac
      721 ccaaatggta accggcaata tcttcatgag aacgacgtcg atagattaat taaacgaagg
      781 tttgaaaaag aaaataaaga gattgtcgca gaactcaaac cagagtcgag ggctaaagag
      841 attactggag aggggcttga aactgctggc aaaggtgcca aagctcttat cgaaactgcc
      901 gagaaaaacc cagtcggtag atttctcatg gggcgtgcta aagcatattt ggaacacata
      961 aaccccaagg acaaagaaca gccttattat tggactggat tcgcgtttgg ctttgggtcc
     1021 aatatagctc taagcttatt cgcaccggtc cttccaatgc ttacaaagcc acttcggtct
     1081 gctatattct caggcggtgt ttttgcaatt tctagaggag taaatctgtt cttaacgaga
     1141 aacttagaaa aaattggaaa tattattgga gctgataata attttgtaaa cgactggtta
     1201 aaaacactaa acaaagctcg tcagcttgaa gcggaaggca aaattgaaga tagcattgaa
     1261 ttacgtaacc aacaatccaa agattctttt ttgaaagctg ggtacaaaga gagaagtgac
     1321 tattgggctt ggacccagga tattggaaga gctgctatga ttgcgcgaat tgaacaagaa
     1381 gtagccaaat acactggatt taaacagaaa ctaaaccata tttcagcagg ctttgcagca
     1441 ggtaacctag gtgctactgg tttccttggt cttgaaaatt tgttttcaga agcttttaaa
     1501 aaaatgcctc atctcgggct ttttggtgga ggtgatgaag cccagatagg ggctcctgaa
     1561 ggtgctgggc ctgaggtacc tcatcacgcc cccactccag gagcacctga acatacaccc
     1621 acccccggcg ttccagaaca cacgcctacc ccaacaccag gagaaattcc tgaaggacct
     1681 ggggtagaac caactcccgg tgttgaacct ggagaagtac ctactgaacc aggcgaggtc
     1741 cctattgaac cagggggcga accaattcca gtgccaatcc ctcctgaggc accaccaatg
     1801 gccatagaag atataataac taatcctgag tacagcagcg cctttgaaat acccgacggc
     1861 gccacgcccg gtcaagtttt tcttaataac gggtttgaag acgcgactct ttgggaagca
     1921 aacgaagcaa atgcgcatct ttttggtgcc cacgtagaag ccaactatga tatgcttgag
     1981 gagatgagca gaaatgttgc cgcttctgga atcccggtcg aagattttcc tgctagtgcg
     2041 gaaataccag gccttatcga agccgcctct gctggagacg ccgaagccat gcacagattg
     2101 acagaagcat tacattattg gccagccggc gacgacttca atctcctaac accagaagga
     2161 gtccaagttg taaataaagc cctagcagca aggatttaat ttaatatggc agatcttgag
     2221 aaagttataa acatattaaa agaaaacaaa gtagaagata aagtaattgc tacttttatt
     2281 gaaggcctta ataatctcct ggctcaaaaa atccaagtag agctaatttc agttttagac
     2341 aacgaaaaag atattgcgag gttaaacgaa cttccgcaag accagatgac tgaagaacta
     2401 gctaaggttt acaaagcaaa agctggaaaa gatatcgcca ttgttagcca agaaattctg
     2461 gacggcttcg tcaccggctt ccttacccag taccacaagc aaaaactgga agaaaaaaag
     2521 tcttgacctc tcacaataga tcgctccgta attcccccga taatcggagt cagtaatccc
     2581 gcgctctgcg accacttcgt gggctagaca aggagactcc atgtcaatcc cccgtttctt
     2641 tactcgctac gatgattact ctgtctggcg gcagtggttt tccttctgga gtacgggcgc
     2701 tggcaaggcc tgaggtcctt gcttcttcga agtgatcaag atccactttt tctagtttca
     2761 ttttttcgcg gattttctca ggggccaaac cctcgaatga ctcccttttt ccagtcccca
     2821 gcatgtttac cgctgaatgt gctatcggaa tcagaacttc atcagcaaat ctttcaaatc
     2881 cccaaccagt agtggacata ataagagctt taactgcatt ttctgtatgt atcatctggc
     2941 tacgaggacc cataccagcg taagccgtat caattttcaa accagctttg gccaccagat
     3001 cttttatttc gtcaggtttg aggaaaacct cgcccacctg tgtagtatga gccatataac
     3061 cagattctgt ttcccactgc ggtggaatta ctccgactga aaaaactagc ttcccatttt
     3121 gggccatcac actagctctc tctctgaggc tttcaccaag tttcgctttc catacctcat
     3181 gagtcccttc agtgacgtaa gtcggacgag ccgccagata accaacctca ccttttttct
     3241 caagtataaa cgccttaatt tctgcgtaaa gattttctag cgattgcagc ggtgtgccca
     3301 aactttctct actcttttca cccacatgag tgagcttgtg tgggtcgaca attcgttcaa
     3361 gagcactttc tttaaatggt agctggtgag gcgacgcgac aattacttgt tgttctttcc
     3421 caagtgtcgc gtgaatacct ttggcttgct ccatgttatc ggtcataaca accacttttt
     3481 cactcgggcc ttgactaact tcggttctcg ggctgccaac aaaaacttca agtgtaaatc
     3541 cggtttgatc tggcgcaagc ttcccttcaa gcctctttac cacggtagct agttcatccc
     3601 tttcccccat cgtttcccac gcttctttct tactccttgt caaaaatccg cgaatctttt
     3661 ctaaaactac ttctatttga ctttgatctt ttttctcttg ctctgccttt ttctgctcga
     3721 atct
//
LOCUS       DASWLN010000120         4322 bp    DNA     linear   ENV 20-FEB-2024
DEFINITION  MAG TPA_asm: Candidatus Zixiibacteriota bacterium isolate
            SMAG_U2949 k141_1069838, whole genome shotgun sequence.
ACCESSION   DASWLN010000120 DASWLN010000000
VERSION     DASWLN010000120.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37960594
            Sequence Read Archive: SRR10376091
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Candidatus Zixiibacteriota bacterium (soil metagenome)
  ORGANISM  Candidatus Zixiibacteriota bacterium
            Bacteria.
REFERENCE   1  (bases 1 to 4322)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 4322)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (26-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/12/2024 08:28:26
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 1,440
            CDSs (total)                      :: 1,416
            Genes (coding)                    :: 1,412
            CDSs (with protein)               :: 1,412
            Genes (RNA)                       :: 24
            rRNAs                             :: 1 (16S)
            partial rRNAs                     :: 1 (16S)
            tRNAs                             :: 21
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 4
            CDSs (without protein)            :: 4
            Pseudo Genes (ambiguous residues) :: 0 of 4
            Pseudo Genes (frameshifted)       :: 0 of 4
            Pseudo Genes (incomplete)         :: 3 of 4
            Pseudo Genes (internal stop)      :: 1 of 4
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..4322
                     /organism="Candidatus Zixiibacteriota bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_1069838"
                     /isolate="SMAG_U2949"
                     /isolation_source="soil"
                     /db_xref="taxon:2053527"
                     /environmental_sample
                     /country="USA: Oregon"
                     /lat_lon="42.99 N 123.63 W"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            <1..379
                     /locus_tag="VGB16_03965"
     CDS             <1..379
                     /locus_tag="VGB16_03965"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=2
                     /transl_table=11
                     /product="Tol-Pal system beta propeller repeat protein
                     TolB"
                     /protein_id="HEX9916872.1"
                     /translation="SSDRAGQPQVYIMSAEGGDVRRLTYEDNYNDSPAWSPRGDKIAY
                     VTRLEGEFQICTIDITGENLIRLTSQGSSENPHWSPDGLHIVFSSNRSGKHQIYTMNW
                     DGSEQKPITGQGNNYNPDWSTGF"
     gene            419..952
                     /gene="pal"
                     /locus_tag="VGB16_03970"
     CDS             419..952
                     /gene="pal"
                     /locus_tag="VGB16_03970"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011937147.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0009279 - cell outer membrane [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="peptidoglycan-associated lipoprotein Pal"
                     /protein_id="HEX9916873.1"
                     /translation="MKKLLLVVVILSLVFLVAGCAGKKTLKPAVEPEISKAPEEKPIE
                     VPTPPEPTKQPEVVSQPKVEKEVIALEMIHFEFDKYRLLPETKTILENNAEMLKAHPK
                     VKVIIEGHCDERGTIEYNLALGEKRALSAKNYLVDLGISADRISTISYGKERPLDPRS
                     NEEAWAKNRRDEFKIVE"
     gene            1095..1757
                     /gene="ybgF"
                     /locus_tag="VGB16_03975"
     CDS             1095..1757
                     /gene="ybgF"
                     /locus_tag="VGB16_03975"
                     /inference="COORDINATES: protein motif:HMM:TIGR02795.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005515 - protein binding [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="tol-pal system protein YbgF"
                     /protein_id="HEX9916874.1"
                     /translation="MRLKQQADYLEQSNYRMEKTIAQLDSLAREQLKQTKSLKADINN
                     SFEEINQRMDILENKIGETRKGSYKTTSENETAAPKDAEGKEVKPDTSKLQINLNPEK
                     LYKSAYLDLTKGNYDLAIMGFSDYLKYFPEADLAPNAQYWIGECYYAKEDFERAVVEF
                     LKVIEKYPQSDKIPSTLYKLGLSYSELKETKTAKAYFDKLIKTYPQSQEAKLAKERQK
                     KP"
     gene            complement(2086..3405)
                     /locus_tag="VGB16_03980"
     CDS             complement(2086..3405)
                     /locus_tag="VGB16_03980"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_010941411.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0022857 - transmembrane transporter
                     activity [Evidence IEA];
                     GO_process: GO:0055085 - transmembrane transport [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="MFS transporter"
                     /protein_id="HEX9916875.1"
                     /translation="MPDNPTGLKFIARALYSRNYRLFFAGQGVSLIGTWMQRIATGWL
                     VYRLTGSAFLLGMVGFAGQIPIFLLSPIAGVLADRWDRRLLIIVTQTLSMLQAFILTF
                     LILTHTIAIWHIIFLSIFLGIVNSFDTPVRQAFVVEMIEKKEDLGNAIALNSSIFNGA
                     RLLGPSIAGILIAAVGEGICFLLNGISYIAVIFALVAMRIKSQKKPIRQTHVLHGLKE
                     GFAYSFGFAPIRSILLLLGLVSLVGMPYAILMPVFAKDVLHGGPSILGFLMAATGVGA
                     LVGALYLASRRSPKGLEKIIALSTTIFGVGLIVFSSSRQIWLSLPLMSLTGFGMMAQM
                     ASSNTILQTITDDDKRGRVMSFYTMSFLGTAPFGSLLAGGLASKIGTPQTLLLGGVCC
                     ILGAFLFTRQLSTLRETIAQAFTRKDGVLEVRRGIQGATELTTPPKD"
     gene            complement(3650..4222)
                     /locus_tag="VGB16_03985"
     CDS             complement(3650..4222)
                     /locus_tag="VGB16_03985"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEX9916876.1"
                     /translation="MIKPKTLLLLLLIVAICMQIRCYSIDYSRAEAYIKRSTTTKNTG
                     NLLFRVEADREEYHLGTPIYLVFSLENADTVPFFVNKRFAVDAFNSPASVREVYLEVV
                     SPAKRGLLFRYLIEKGYVVRENFVCLCPGEKIYSSKFLLNDDYEIDEVGKYKIVGVYE
                     NYWGPEFGFRNAWMGKVESEPVEFEVLPIE"
BASE COUNT         1341 a          939 c          967 g         1075 t
ORIGIN      
        1 ttcctcagac cgcgcaggac aacctcaagt ctatattatg agcgctgaag ggggagatgt
       61 gagacgctta acttatgagg ataattacaa tgattcgccg gcttggtctc cgcgtgggga
      121 taaaattgcc tatgtgactc gactagaggg cgaatttcaa atatgcacca tagatatcac
      181 cggagagaat ttgatccgct tgacttcaca aggaagcagt gagaatccac actggtcgcc
      241 ggatggttta cacatagtct ttagttcaaa tcgtagcgga aaacatcaaa tctatactat
      301 gaattgggat ggctcggaac agaaaccgat aactggtcag gggaataact ataatccaga
      361 ttggtcaacc ggattctaat ataaatcact aatctctaaa ttccaaagga ggtgaaaaat
      421 gaagaaatta cttttagtgg tagtaattct ttcccttgtt ttcttggtgg caggatgtgc
      481 tggaaagaag accctcaaac cagcagtgga gccggagata agcaaagctc cagaggagaa
      541 accgattgag gtgccaaccc caccagagcc aactaagcag ccggaggttg tttctcagcc
      601 gaaggttgaa aaggaagtta tagctctgga gatgattcat tttgaattcg acaagtatcg
      661 cttgcttcca gaaactaaga ctattctgga aaacaatgct gagatgctga aagctcaccc
      721 caaggtcaaa gttataattg aagggcattg cgacgagcgg ggaacaattg aatacaactt
      781 agctttgggt gagaaaagag ccctttcagc caaaaactac ttagtcgatt tgggaatcag
      841 tgcagatagg atttccacta taagttatgg caaggaaaga ccactcgacc ctcgttccaa
      901 cgaggaagca tgggcgaaaa acagaagaga tgagttcaag atagtggaat agtcgaaaca
      961 atcgggacac ctgcccaaaa gcgggtgtcc cttttacaag aaaggcaggg agcttgagca
     1021 agaagacaat aattctcctc tgcgcattaa tatttttact ggtgtttgga ggatgtgcca
     1081 gccgccaaga gattatgaga ttaaaacagc aagcggacta tctggagcaa agcaattaca
     1141 ggatggaaaa aaccattgcc cagctggaca gcttggcaag ggaacagttg aaacagacga
     1201 aaagtctgaa agctgacatc aacaactcct ttgaagagat taatcagagg atggatatcc
     1261 tggagaataa gataggggag acgaggaagg gctcatataa aactacgagc gaaaatgaga
     1321 ctgctgctcc aaaggatgcc gaggggaagg aagtcaagcc cgatacctct aagctccaga
     1381 taaatctaaa tccagaaaaa ctttacaagt ccgcttatct tgatttgacc aaagggaatt
     1441 acgatttagc tattatggga ttttccgatt atctgaagta ttttcctgag gcggatttgg
     1501 ctcccaatgc tcaatactgg atcggggaat gttattatgc caaggaggat ttcgaacggg
     1561 cagtggtgga atttctcaag gtcatagaga aatatcctca aagtgacaaa atccccagca
     1621 cactttacaa gttgggctta tcctattcgg agcttaagga gaccaagacc gcaaaggcat
     1681 actttgacaa gctgattaag acatatcccc aatcgcaaga agctaagctg gcaaaagaga
     1741 gacagaagaa accttaaaga taatatataa ctatagttgt cccgatttat cggggcggta
     1801 ttatagaacc tcacccattc cctctccttg gaaaggagag ggttaggaga ggttattttt
     1861 atatctatgt ggggactctg ttggttcggg aattggaatt cccgagccca tcttctgttt
     1921 cccacataaa cgcagaaaat aaatacgcgt aggggtggag tttatctcca cccagaactc
     1981 ttcgggcgaa gacaagcttc gcccctacgc ggaactaaaa aatgcgggcg acccgccggg
     2041 tcgcccgcat ttcatattct cacgaaatgc cagttgacct attgcctaat ctttgggtgg
     2101 tgtagttagt tccgttgctc cctgaatccc ccttctcact tccaaaaccc catcttttct
     2161 tgtaaatgct tgagcaatcg tttcccttaa agtcgaaagt tgccgggtga acaaaaatgc
     2221 acctaagatg cagcaaactc cacctaatag tagggtctgt ggagtgccta ttttgctggc
     2281 taatccccca gccaacagac taccgaaagg ggctgtcccc aaaaaggaca tagtgtaaaa
     2341 gctcattacc cgaccgcgtt tgtcatcatc cgtaatggtc tgtaaaattg tattgctgga
     2401 tgccatctgc gccatcatcc cgaaaccggt caaagacatc aatggcaaag aaagccatat
     2461 ttgacgagac gaagagaaaa cgataagtcc gacaccaaag atggtagtgg aaagcgcaat
     2521 aatcttttcc aatcctttag ggcttctacg tgaggcaagg taaagagcgc ccaccagtgc
     2581 gcccacgccg gtagctgcca taagaaaacc gagaatgctg ggaccaccat gcagaacgtc
     2641 tttagcaaaa accggcatta atatggcgta aggcatcccc accagactga ccaatcccaa
     2701 aaggagcaaa atggagcgaa taggggcgaa gccgaaggag taagcgaatc cttcttttaa
     2761 cccatgtaga acatgggttt gtcggattgg ctttttttgg gacttgattc tcattgctac
     2821 cagagcgaat atgacagcta tataactgat gccattaagc agaaaacaaa tcccttcacc
     2881 cacagcagcg atgagtatgc cggctatgga aggacccaat aagcgagcgc cattgaatat
     2941 tgaggaattt agggcaatag catttcctaa atcctctttc ttctcgatca tctccacaac
     3001 aaacgcctga cgcaccggtg tgtcaaaaga gttcacaata cctaagaaaa tgctcaaaaa
     3061 tatgatatgc caaatggcga tggtgtgggt taaaatgagg aatgttagaa tgaatgcctg
     3121 tagcatagat agggtttggg taacaattat gagaagacgg cgatcccacc tgtctgctaa
     3181 cacgccggct atgggggata agagaaagat gggaatttga cctgcaaatc ccaccattcc
     3241 caataaaaac gcagaaccag tcaagcgata aaccagccag cctgtggcta tcctctgcat
     3301 ccaggtgcca attaaggaaa cgccctgccc ggcaaaaaac aggcgatagt ttctggaata
     3361 caatgcgcga gcaatgaatt ttaaaccagt gggattgtca ggcatgagtc aataaatttt
     3421 tcctcattaa caagttgtgt caggagctat tcctgacaca accttcgcca agtctaagga
     3481 cttggaagga gtaaataatt agtctaataa ttggacagct acaaaaaagc tcatattaaa
     3541 atgagattgc ttcgtcgctt cgcttctcgc aatgacatta tgatggtgtc attgcgagcg
     3601 accgtaggga gcgtggcaat ctctttgttc gtctgtgtta ttatatttat cattctattg
     3661 gtaacacctc gaactcaact ggctcacttt ctactttccc catccaagcg tttctaaagc
     3721 caaattcagg accccagtaa ttttcataga ctcccacaat cttatatttt cctacttcgt
     3781 cgatctcata gtcgtcattt aagagaaatt tggaagagta gattttttct ccggggcaga
     3841 gacagacaaa attctccctt accacgtatc ccttttctat aaggtatcga aataacagac
     3901 ctctcttagc aggtgataca acttcaagat aaacttccct tacggaagcg ggcgaattaa
     3961 aagcatcaac tgcaaacctc ttattaacga agaaaggtac ggtatctgca ttctcgaggg
     4021 agaaaaccaa gtaaataggt gtcccaagat gatattcttc tcggtctgcc tctacacgaa
     4081 ataagaggtt acctgtattc ttagtcgttg tgctcctctt gatatatgct tctgcccgtg
     4141 aataatctat tgaataacac cgaatctgca tacaaatagc aactattaac aaaagcaata
     4201 acagggtttt aggttttatc atttcttaag aatcctctta aatctcagct tccctcaact
     4261 gttttgctgc tgtttcaggg gtgatttcgt tgatataata tccgcttccc cactcgaatc
     4321 ca
//
LOCUS       DASWLN010000121         6619 bp    DNA     linear   ENV 20-FEB-2024
DEFINITION  MAG TPA_asm: Candidatus Zixiibacteriota bacterium isolate
            SMAG_U2949 k141_1070330, whole genome shotgun sequence.
ACCESSION   DASWLN010000121 DASWLN010000000
VERSION     DASWLN010000121.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37960594
            Sequence Read Archive: SRR10376091
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Candidatus Zixiibacteriota bacterium (soil metagenome)
  ORGANISM  Candidatus Zixiibacteriota bacterium
            Bacteria.
REFERENCE   1  (bases 1 to 6619)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 6619)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (26-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/12/2024 08:28:26
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 1,440
            CDSs (total)                      :: 1,416
            Genes (coding)                    :: 1,412
            CDSs (with protein)               :: 1,412
            Genes (RNA)                       :: 24
            rRNAs                             :: 1 (16S)
            partial rRNAs                     :: 1 (16S)
            tRNAs                             :: 21
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 4
            CDSs (without protein)            :: 4
            Pseudo Genes (ambiguous residues) :: 0 of 4
            Pseudo Genes (frameshifted)       :: 0 of 4
            Pseudo Genes (incomplete)         :: 3 of 4
            Pseudo Genes (internal stop)      :: 1 of 4
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..6619
                     /organism="Candidatus Zixiibacteriota bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_1070330"
                     /isolate="SMAG_U2949"
                     /isolation_source="soil"
                     /db_xref="taxon:2053527"
                     /environmental_sample
                     /country="USA: Oregon"
                     /lat_lon="42.99 N 123.63 W"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            complement(<1..630)
                     /locus_tag="VGB16_03990"
     CDS             complement(<1..630)
                     /locus_tag="VGB16_03990"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="polymer-forming cytoskeletal protein"
                     /protein_id="HEX9916877.1"
                     /translation="MIKTKSIWAFLFIILIFFFSGTFVSRSQSASIKEKAAEVKVEDG
                     EVYIKTVEGKEVKISEEGSISVGEESSQTQESTESQSEEGKKTIDQIVRIGGDVVVEE
                     DEIVREDVVTIGGSITVKGKVDGDVTAVGGIIRVTSTGIIEGDATGVGGGVEREPGGV
                     INGDATDISFPLIGCGALPRSHLLGQQPFHPAFAIRGLMLFARIIKIMIF"
     gene            complement(640..1710)
                     /locus_tag="VGB16_03995"
     CDS             complement(640..1710)
                     /locus_tag="VGB16_03995"
                     /inference="COORDINATES: protein motif:HMM:NF024743.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF4097 family beta strand repeat-containing
                     protein"
                     /protein_id="HEX9916878.1"
                     /translation="MKVVSSILTLLLCISFLSTVYARAEEFSFELEKVFEVGKTENFI
                     KLSNSSGEVTIESHNQDKIIVHATKVIRAKDQKEANQIAERIVIDINKTDSTITIETE
                     YPKIRPKGFWSNFLRFDWGDRAWVDYHILVPAKTKLDIHSASADVNVRGIENQVDVWV
                     ASGDVLLENIIGDVNANSASGNLEVYGAQGRINLKGASSDIKIRRTEGSMNLTTASGD
                     IYGEKLEGNLFISQASGDLELYEINGDITAKGASGDKRIEQKQGSLNLNSSSGDIEAK
                     TDILPNKEYLIESASGDVKLYIPLQSKADIELRTASGNIDCKIPMELRIVTSKKIEGK
                     ANQGGSQITIETTSGDIFLFSQ"
     gene            complement(1719..2285)
                     /locus_tag="VGB16_04000"
     CDS             complement(1719..2285)
                     /locus_tag="VGB16_04000"
                     /inference="COORDINATES: protein motif:HMM:TIGR02937.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="sigma-70 family RNA polymerase sigma factor"
                     /protein_id="HEX9916879.1"
                     /translation="MLDDDQILVERALTGDQKAFRLLLKKHQSAIYHVIFKLVHNQEE
                     AWDLVQETFMKAFRSLSSYKKEYRLSTWLYRIAANCSIDYLRKRKIEALSLDQPLETK
                     EGSVCLELPDFTYNPESDFESRQKGISINQAIESLPRKYKEVIIYRHKDYKSYEEISR
                     ILGIPVGTVKARIFRARELLKKRLKSLR"
     gene            2657..2797
                     /locus_tag="VGB16_04005"
     CDS             2657..2797
                     /locus_tag="VGB16_04005"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEX9916880.1"
                     /translation="MAKKKKEEYELPEKIYWHIYSLQNLNAEKFVRVVDKILRKEYIL
                     IK"
     gene            2884..3795
                     /locus_tag="VGB16_04010"
     CDS             2884..3795
                     /locus_tag="VGB16_04010"
                     /EC_number="3.4.-.-"
                     /inference="COORDINATES: protein motif:HMM:NF013700.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0046872 - metal ion binding [Evidence
                     IEA];
                     GO_process: GO:0006508 - proteolysis [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="M23 family metallopeptidase"
                     /protein_id="HEX9916881.1"
                     /translation="MKKRFSFIIIPHDKGKVFETKISLPLICILISLLAFLLIINSYF
                     VIDSLNKTYQNNKLTELEKENRYLEKKLEGFNSLVSQLKGEVKSLIQNEKKIRLVFGL
                     PEIDDEVREVGIGGPTGFDYRLTSPTLKKVSLTEEELDKLLRQSKFERESFEEIYSQL
                     LDKKMVLDHTPSITPVIGYFSRGFGMKKDPFTGMMQFHRGIDLAADRDTPIYAPANGS
                     IIYIGHESGMGKVIRIAHGYGYETVYAHLRSTKVARGQMVKRGDLIATVGNTGYSTGP
                     HLHYEVHYQGQAVDPLNYVLCSSDFLD"
     gene            3858..4298
                     /locus_tag="VGB16_04015"
     CDS             3858..4298
                     /locus_tag="VGB16_04015"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_017752868.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF523 domain-containing protein"
                     /protein_id="HEX9916882.1"
                     /translation="MKPKYDYIVSACLAGLNTRFDGTSCKDRKVSELVKRGRAIPVCP
                     ELLGGLSVPRFPAQIVEGEGRDVLNGRSRVTNQEGEDVTEFFTKGAYRFLEIAKLFNI
                     RKAVMKDESPSCGCGRVRCSRRTIQGDGVTTSLLRSNGLDVICL"
     gene            4354..5001
                     /locus_tag="VGB16_04020"
     CDS             4354..5001
                     /locus_tag="VGB16_04020"
                     /inference="COORDINATES: protein motif:HMM:NF019265.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003677 - DNA binding [Evidence IEA];
                     GO_function: GO:0003700 - DNA-binding transcription factor
                     activity [Evidence IEA];
                     GO_function: GO:0016987 - sigma factor activity [Evidence
                     IEA];
                     GO_process: GO:0006352 - DNA-templated transcription
                     initiation [Evidence IEA];
                     GO_process: GO:0006355 - regulation of DNA-templated
                     transcription [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="sigma-70 family RNA polymerase sigma factor"
                     /protein_id="HEX9916883.1"
                     /translation="MSTLDDNAKRDIILEKSVDSDKALLEEERGLIEGAKRGDQLSFK
                     KLMDKYKSQVGGLAYRMVGSYEDARDISQNVFIKAYQNLGSFDTKRKFSTWLYRIAMN
                     ASIDYLRKYRKHKTDHLDDLAGQLSDKKDGPEKVFNNTLVKLAVRDSLESLNSKQKSV
                     FVLRDLEGLDINEIAQITQMPQATVRWYLHRARAKLKGELIKHHPLVLKRMGVRL"
     gene            4998..5468
                     /locus_tag="VGB16_04025"
     CDS             4998..5468
                     /locus_tag="VGB16_04025"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEX9916884.1"
                     /translation="MKCGKAKKLINGYAELKETNKNSLDSHVAGCPSCQRGLELHKAS
                     INMFKQALSFEDKGMSWEGFVEQAPLEAQHLSWLSSIREKMKELLRLIATPVLGPVPA
                     YVFSLFLILVAGLGAYSSLSREKILVLENVIVLDREYLSSVDDGEKTVYLVNRK"
     gene            5465..6514
                     /locus_tag="VGB16_04030"
     CDS             5465..6514
                     /locus_tag="VGB16_04030"
                     /inference="COORDINATES: protein motif:HMM:NF024578.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005515 - protein binding [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="PDZ domain-containing protein"
                     /protein_id="HEX9916885.1"
                     /translation="MRKFNLIRLHLRYLLGVGAIFFLPFLAGCQKNGLEALERDVIKL
                     RQKVGQYVVCLVANNVKNKSVKLGSGIIVDENHILVTENFLGDIGKMRIMLQDGRIIE
                     DSQIVNACCDFETNLSLLEVKGVKLRPVERIFKGKPESGTLGVALFNSQYSKGLCAIL
                     GSVNPSWIGGDDIYDEELLIFNPLSGPLYSGTPVFNTKGELLGLVEGRLEEKDGMVLL
                     VPANTCQKVSKVLRENGRIERGWIGIASDKACKNEKLARIMEKSAKGILITEVVEKSP
                     AQTCGLKPGDIIMRCNGEEVNCKTEFRKKVSSQTIGSSLVLGVVRQGKEMDFKVKVEP
                     RPEVPAKRRCPNRPI"
BASE COUNT         1935 a         1324 c         1455 g         1905 t
ORIGIN      
        1 aaaaatcata atttttatta tccgagcgaa aagcatcaat cccctgatgg caaaagcagg
       61 atgaaagggc tgctgcccaa gaaggtggga acgggggaga gccccgcagc ctattaacgg
      121 gaaactaatg tctgtagcat ctccattaat tactccccca ggctctcttt caactcctcc
      181 acccacgccc gtcgcatcgc cctctataat tccagtcgag gttacgcgaa ttattcctcc
      241 aaccgctgtc acatcaccgt ccacttttcc ttttacggtt atacttcccc caatggtaac
      301 cacatcctct ctcactattt cgtcttcctc caccaccacg tcacccccta ttctaacgat
      361 ctggtcgata gtttttttgc cttcttcgga ctgactttcc gtggactcct gggtttgact
      421 tgattcttct cccactgaga tagagccttc ttcggatatc ttaacttcct taccttcaac
      481 agtcttgata taaacttcac catcttccac tttcacttca gccgccttct ccttgattga
      541 ggcggattgg cttcggctaa caaacgtccc gctaaaaaag aaaatcaaga tgataaatag
      601 aaaagcccaa attgattttg tttttatcat tttctcctcc tattggctaa aaaggaaaat
      661 atcgccactg gtggtttcaa ttgttatttg agacccaccc tggtttgctt ttccctctat
      721 tttcttagag gtgacaatac gcaattccat aggaatttta caatctatat tgccacttgc
      781 tgttctcaat tcgatatcag ctttggattg tagggggata tataatttca catccccgct
      841 ggcactctct ataagatatt ccttattcgg caggatgtca gttttggctt caatatctcc
      901 actgctgcta tttaagttaa ggctaccttg tttctgttca attcttttat ctccggaagc
      961 gcccttagca gttatatccc cgtttatctc gtataattcc aaatctccgc tagcttgcga
     1021 gataaataga ttcccctcga gtttctcacc atagatatcc ccgctggcgg tagttaaatt
     1081 catacttcct tcagttcttc ttatcttgat atctgagctg gcccccttaa ggttaatcct
     1141 cccttgagca ccatagacct ccagattgcc actggcggag ttagcattta catctcctat
     1201 tatattttcc aatagaacat ctcctgaagc tacccagaca tctacctggt tttctattcc
     1261 tcttacgttc acatctgctg atgccgaatg tatgtctaat ttggtcttag ctggaacaag
     1321 gatatgataa tccacccaag ccctgtctcc ccaatcaaat cttaagaaat tgctccaaaa
     1381 accttttggt ctaatcttgg gatattcggt ctcaatggtt atggttgaat cggtcttgtt
     1441 aatatcaatt actatccttt cagcgatctg gtttgcttcc ttttgatcct tagctctgat
     1501 tactttcgtg gcgtggacga tgattttgtc ctggttgtgc gactcaatag tgacctcacc
     1561 cgaactattg cttaatttga taaagttctc tgttttccca acttcaaata ctttttccaa
     1621 ctcgaagctg aactcctcag cccgagcata aacggttgaa agaaaggata tacacaagag
     1681 caatgtcaaa atggaactga caactttcat acttcctcct atctcaatga tttcaatctt
     1741 ttcttcaaca actcccttgc cctgaaaatg cgagccttaa cagttcccac cggtattccc
     1801 agaatcctgg atatctcctc atatgatttg taatccttat gccggtagat tatcacctct
     1861 ttatactttc taggaagcga ttctatggct tgattgatgg agattccttt ttgtctggat
     1921 tcaaaatcag attcaggatt ataggtgaaa tccggcagtt ccagacaaac gcttccctct
     1981 ttagtttcca aaggctggtc cagggagagt gcttcgattt ttctttttct caaatagtcg
     2041 atggagcagt tagccgcaat cctatacagc caggtgctaa gacgatattc cttcttatag
     2101 cttgacagag agcgaaaagc tttcatgaag gtctcttgaa ccagatccca agcctcctct
     2161 tgattatgca ccagcttgaa tatcacatga tagatagcgg attggtgttt tttcaaaagg
     2221 agacggaaag ctttctggtc tccggttaaa gcccgctcca ctagaatctg gtcgtcatcc
     2281 aacactttcc ctgccttttt gggatacgtc aaaataaggt ggttgtttca aaaatatcat
     2341 aatccaatgg aaaacacaaa caaaaaaaat tggattcgac gcatataggg taaagcccac
     2401 attctattgt tttcagtcaa tattattatc cgaagactta aaggataaag gttggcttta
     2461 atagtttaat cagattaaca acaataggtt aggttatttc cttaccttgg gtgatttagg
     2521 cattgaggtt gctacaaaga aagtggaatt aggttcagaa ttcttaaact tatgccgata
     2581 gttgtaataa ggcaggtttc gacttccaac cccccaacaa aacttattac agctcgacag
     2641 acaaggggga atggatatgg caaagaagaa aaaagaggag tacgaactgc cagagaaaat
     2701 ttactggcac atctacagct tgcagaatct taacgcagag aagttcgttc gggtggttga
     2761 caaaatcttg agaaaagaat acatcttaat caaatgagga aaaaactgct tgacacgcaa
     2821 atgctaattc catatagtta gataatatct cagtgagcaa tcctttcaag gtagggcccc
     2881 cctatgaaaa agagattctc ctttataata attccgcacg acaagggcaa ggtcttcgaa
     2941 actaaaatct ctctcccttt gatatgcata ctgatttccc ttttagcctt cttgctcatc
     3001 atcaactcgt atttcgttat agactctttg aataagactt accagaataa caaattgacg
     3061 gaattggaaa aagaaaatcg ctatctggag aagaaactcg aaggtttcaa ctcgctggtt
     3121 tcccaattga aaggtgaggt gaagagttta atccagaatg aaaagaagat tcggttggtc
     3181 tttgggctac cggaaataga tgatgaggtg agggaagtag gtataggtgg tcccacgggt
     3241 tttgattaca gactcaccag cccgactttg aaaaaggtta gtttgactga ggaggaactg
     3301 gataagctcc tgagacaatc caagttcgaa agggagagtt ttgaagagat atattcgcaa
     3361 cttttggata agaagatggt cttagaccac accccttcaa ttacgccagt gattggttat
     3421 ttcagtcgcg gattcgggat gaaaaaagac ccctttactg gtatgatgca gtttcatcga
     3481 ggtatagact tggcagcaga ccgggatact ccgatttatg ccccggctaa tgggagtatt
     3541 atctatattg gtcatgaatc ggggatgggc aaagtgataa ggatagcaca cggttatggt
     3601 tacgagacag tttatgctca cctacgaagt acaaaggttg cccgggggca aatggtgaaa
     3661 aggggtgacc ttattgccac agtaggtaat accggttatt caactggccc acacctgcat
     3721 tatgaagtgc attatcaggg gcaagcagtt gacccgctaa actatgtcct ctgcagttca
     3781 gactttttgg attgaaactg tatacttgat tagtcctcca gtcctcgagc ttctcgagga
     3841 tttttttatg acttgatatg aagccaaaat atgattatat tgtctccgcc tgcttggccg
     3901 gcttgaatac aagattcgat ggcaccagct gcaaggacag aaaagtatct gaactggtga
     3961 aaagaggtag agcaatacca gtctgccctg aactcctggg cgggttatct gttcctcgtt
     4021 tccctgcgca aatagttgag ggtgagggaa gagatgttct aaatggcaga agcagggtga
     4081 caaaccaaga aggagaggac gtaactgaat tcttcaccaa aggggcttat cggtttttag
     4141 aaatagctaa gttgtttaat ataagaaaag cggtaatgaa ggatgagagc ccttcctgtg
     4201 gttgcggccg agttcgttgc tcaaggagaa ctattcaagg agatggagta acaacttcgc
     4261 ttttaagaag caatggcttg gatgtgatct gtctatgatt aaattctcaa atgacttaga
     4321 gtaacaatcg tataaaaaag agagggctgg cggatgtcca ctttggacga caatgccaaa
     4381 agagatatca tcttagaaaa atcagttgat tccgacaagg ctcttttgga agaagaaaga
     4441 gggttaattg agggagctaa aagaggtgac cagctttctt tcaagaagct aatggataag
     4501 tacaagtcgc aggtgggagg tctggcttat agaatggtgg gaagttatga agatgcccgc
     4561 gacataagtc aaaacgtttt tattaaggct tatcagaatt taggaagctt tgataccaaa
     4621 cggaagtttt ccacttggct ttatcgtatt gccatgaatg ccagcataga ttacttaaga
     4681 aaataccgaa agcacaagac ggaccactta gatgacttgg ccggacaact aagcgacaag
     4741 aaagatggac cggaaaaggt tttcaacaac accttagtaa aattggcagt gcgggatagc
     4801 ctggagagtt taaactctaa acagaaatcg gtatttgtct tgcgcgattt agagggttta
     4861 gacataaatg agatagccca gataactcag atgccccaag ctacagttcg ctggtatctg
     4921 catagggcta gggctaagtt aaaaggagaa ttaatcaaac atcatccctt agtcttgaag
     4981 aggatgggag ttagattatg aagtgcggga aagccaaaaa actaatcaat ggctatgcgg
     5041 aactaaagga aactaataag aacagtctgg attcgcatgt tgcaggttgt ccttcttgtc
     5101 agagagggtt ggagctgcac aaagcttcaa tcaatatgtt caaacaggca ctttcttttg
     5161 aagataaggg gatgtcttgg gaaggatttg ttgagcaggc ccctttggaa gcacagcatc
     5221 tctcttggct tagttcaatt agagaaaaaa tgaaggagct tttgcgactt atagcaacac
     5281 ctgttttggg tccggtgcca gcttatgtct tttccctgtt tctaatcctt gtggctggct
     5341 taggggctta ctcatctcta tccagagaga aaatcttagt tttggagaat gtgatagtat
     5401 tggatagaga atatctttcc tccgtggacg atggggagaa aaccgtttac ctggtgaatc
     5461 gaaaatgaga aaatttaact taatcagact tcatcttagg tatctattag gagtaggtgc
     5521 aatttttttc cttccttttt tggcaggttg tcaaaaaaat ggcttggaag ctttggaaag
     5581 agatgtaatc aaactcaggc agaaggttgg tcagtatgtg gtttgtttag tagctaataa
     5641 tgtaaagaat aaatctgtta agttaggcag tgggataata gtggatgaaa atcatatttt
     5701 ggtcacggaa aactttctgg gagacatagg caagatgaga ataatgctac aggatggaag
     5761 aatcattgaa gatagccaga tagttaatgc ctgttgtgat tttgagacca atctcagtct
     5821 cttggaagtg aaaggtgtga agcttaggcc cgtggagaga atatttaaag ggaaaccaga
     5881 gagtgggact ttgggtgtgg ctttgtttaa tagtcaatac tccaagggtt tgtgtgcaat
     5941 cttaggttca gttaatcctt cctggatagg cggggacgat atttacgatg aagagctctt
     6001 gatctttaat ccactctccg gtccacttta tagcggaaca ccagtattca ataccaaagg
     6061 cgaacttctg ggattggtcg aaggtagatt ggaagagaag gacggaatgg ttctgcttgt
     6121 gccagccaat acctgccaga aggtgagtaa ggtcttgagg gagaatggaa ggatagagcg
     6181 cggctggatc ggaatagctt cagacaaggc ttgcaagaac gaaaaattag cgaggataat
     6241 ggaaaagtcc gccaagggta ttcttatcac tgaagtggta gaaaaaagtc cagcccaaac
     6301 ttgtggttta aagccgggag acattattat gagatgtaat ggggaggagg ttaactgcaa
     6361 gaccgagttt aggaaaaagg tatcaagcca gaccatcggc tccagtctgg tcttaggcgt
     6421 agtccgtcaa ggaaaagaaa tggatttcaa agtaaaagtt gaacctcgac ccgaagttcc
     6481 cgccaaaaga agatgtccca atcgtccaat ctaatggaac agagctcaat cctcatttgt
     6541 ataataaaga taaatcgtta attatagcaa acgcattaaa tgtcgttaca tattagagga
     6601 atatgcaatc tattttaaa
//
LOCUS       DASWLN010000122        11973 bp    DNA     linear   ENV 20-FEB-2024
DEFINITION  MAG TPA_asm: Candidatus Zixiibacteriota bacterium isolate
            SMAG_U2949 k141_1191377, whole genome shotgun sequence.
ACCESSION   DASWLN010000122 DASWLN010000000
VERSION     DASWLN010000122.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37960594
            Sequence Read Archive: SRR10376091
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Candidatus Zixiibacteriota bacterium (soil metagenome)
  ORGANISM  Candidatus Zixiibacteriota bacterium
            Bacteria.
REFERENCE   1  (bases 1 to 11973)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 11973)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (26-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/12/2024 08:28:26
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 1,440
            CDSs (total)                      :: 1,416
            Genes (coding)                    :: 1,412
            CDSs (with protein)               :: 1,412
            Genes (RNA)                       :: 24
            rRNAs                             :: 1 (16S)
            partial rRNAs                     :: 1 (16S)
            tRNAs                             :: 21
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 4
            CDSs (without protein)            :: 4
            Pseudo Genes (ambiguous residues) :: 0 of 4
            Pseudo Genes (frameshifted)       :: 0 of 4
            Pseudo Genes (incomplete)         :: 3 of 4
            Pseudo Genes (internal stop)      :: 1 of 4
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..11973
                     /organism="Candidatus Zixiibacteriota bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_1191377"
                     /isolate="SMAG_U2949"
                     /isolation_source="soil"
                     /db_xref="taxon:2053527"
                     /environmental_sample
                     /country="USA: Oregon"
                     /lat_lon="42.99 N 123.63 W"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            <1..402
                     /locus_tag="VGB16_04035"
     CDS             <1..402
                     /locus_tag="VGB16_04035"
                     /EC_number="1.1.1.205"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_018206300.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003824 - catalytic activity [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="IMP dehydrogenase"
                     /protein_id="HEX9916886.1"
                     /translation="HAVMIGGLFAGLEESPGDRIVFEGRSYKIHRGMGSLEAMRSGGK
                     DRYFQEHEAELKKLVPEGVEGRVPYKGSLSDAVYQLIGGVRAGMGLCGARNAEELRKK
                     GEFIQITYAGLRESHPHSVIITKEAPNYWVS"
     gene            498..877
                     /gene="rnpB"
                     /locus_tag="VGB16_04040"
     ncRNA           498..877
                     /ncRNA_class="RNase_P_RNA"
                     /gene="rnpB"
                     /locus_tag="VGB16_04040"
                     /product="RNase P RNA component class A"
                     /inference="COORDINATES: nucleotide
                     motif:Rfam:14.4:RF00010"
                     /inference="COORDINATES: profile:INFERNAL:1.1.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: cmsearch."
                     /db_xref="RFAM:RF00010"
     gene            941..1336
                     /locus_tag="VGB16_04045"
     CDS             941..1336
                     /locus_tag="VGB16_04045"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002714404.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003677 - DNA binding [Evidence IEA];
                     GO_function: GO:0004519 - endonuclease activity [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="endonuclease domain-containing protein"
                     /protein_id="HEX9916887.1"
                     /translation="MKRNNIGRCRALRKNQTDAERKLWSILRNRQLGGAKFRRQCSVD
                     RFILDFYCPECKVGIEADGGQHYKKCSRKQDELRTKELNKLGIKIMRFTDREILVNTD
                     GVYEILKTAIDKKRAGPPHLYPLPKGERR"
     gene            1535..1999
                     /locus_tag="VGB16_04050"
     CDS             1535..1999
                     /locus_tag="VGB16_04050"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEX9916888.1"
                     /translation="MKLCVLLLIVLMFVLSGCSIWYDFMSVVGDHGYRVCAFIRGVVV
                     DSSTHKPLKDVSVKIYRYKYLEYQDITDSAGMYGHNKVELLFSSTSKEISELRKKPPI
                     RKKIELRMVFEKTGYRKHELLVPVEFVCCSLHPPQEVTISEVKIEDVYLVKE"
     gene            2064..3773
                     /gene="recJ"
                     /locus_tag="VGB16_04055"
     CDS             2064..3773
                     /gene="recJ"
                     /locus_tag="VGB16_04055"
                     /inference="COORDINATES: protein motif:HMM:TIGR00644.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008297 - single-stranded DNA
                     exodeoxyribonuclease activity [Evidence IEA];
                     GO_process: GO:0006310 - DNA recombination [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="single-stranded-DNA-specific exonuclease RecJ"
                     /protein_id="HEX9916889.1"
                     /translation="MSLKWEFAPKPEEKLVAEISSQLDLNPIVTRILVSRNIDTSAKI
                     KRFLNSSLNDLCDPYLLGQTDIAVDRIIRALRENEKMMIFGDYDVDGITATALMFLVL
                     NRLGGEVSYYLPNRLLEGYGLSEDGILEAEQRGVRLIITVDCGVTAYEEINFARQKGI
                     DCIITDHHEPGDKHLDAVAVINPKQKGEDGLAGELSGVGVAFKLAQALYQKLEQDQTE
                     LEDHLDLVALGTAADIVPLTGENRILTKFGIKQITKTTKPGLKSLIFVSGIMGKEINT
                     GQVMFLLAPRINAVGRLGDAEKAIKLLTTKDERLAAELARFLDAENKKRKDIDEGTLG
                     QALEMVEKEVNLDEDRAIVLSSNNWHPGVIGIVASRIVERYHRPTVMISIDKDEGKGS
                     ARSIASFHLYDALKECDDLLLKYGGHKYAAGLSILPEKIPQFKERLKEVAHRKLKADD
                     LIPGLYVDAEIELEQIDDNLSDLLEKFSPFGPQNNRPVFVTRNLEVMGQPYVVGNNHL
                     KLKVRKGGVVFDVIGFGFGDWIKPLSMTKIGVDLAYVVEKVEWNGESKTQLRIKDIKF
                     SGS"
     gene            3775..4719
                     /gene="meaB"
                     /locus_tag="VGB16_04060"
     CDS             3775..4719
                     /gene="meaB"
                     /locus_tag="VGB16_04060"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011026419.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016772 - transferase activity,
                     transferring phosphorus-containing groups [Evidence IEA];
                     GO_process: GO:0015819 - lysine transport [Evidence IEA];
                     GO_process: GO:0015822 - ornithine transport [Evidence
                     IEA];
                     GO_process: GO:1903826 - L-arginine transmembrane
                     transport [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="methylmalonyl Co-A mutase-associated GTPase
                     MeaB"
                     /protein_id="HEX9916890.1"
                     /translation="MNVLKRFYRGEKTALAKLISYVENQESDYEKILAQLYPQANKAY
                     HIGITGPPGAGKSTLVDKLTAILLKDNKRIGIIAVDPTSPFTGGALLGDRIRMQDLTN
                     KEGVFIRSMATRGSFGGLATSTKEVSLILDAFGKDYIIIETVGVGQVELDIVNACDTT
                     IVVLVPESGDSIQAMKAGLMEIADIFVVNKSDREGSERIISELNMILDIRRKKGEWEY
                     PVVPTKAVNNEGIGQLLERIESHRRFITGNSIFEKNRKKQIKNELAKILENKLRKLLE
                     KDILKDFNLDQMAEEIYQGKDNPYDIGEKILKQVDFSR"
     gene            4737..5405
                     /locus_tag="VGB16_04065"
     CDS             4737..5405
                     /locus_tag="VGB16_04065"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEX9916891.1"
                     /translation="MLSGIEGLLLLCWDWNWANFVVNLLVGVGTIGVVLLVLYQEFWR
                     TPKLEVEFDQNKEGFCRYASVNWVIKHIPNRLVYCEIDQSVLTYWVRIKVINIGKTVA
                     KNVVGRVNEMFCIKDNKVTFCQPFDPCILHWVGQKGDDLNLIDVNKKEYYYLDILFIE
                     KDDPCVYINTDLNQTRGIKYNWEAGDYLLKIALYSENAKPVSKTLRVVWNGKWNEIEV
                     SLSR"
     gene            5638..7308
                     /locus_tag="VGB16_04070"
     CDS             5638..7308
                     /locus_tag="VGB16_04070"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_018085043.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="methylmalonyl-CoA mutase family protein"
                     /protein_id="HEX9916892.1"
                     /translation="MYQESFKQKIRKKKEEWDGKFKKCRNREEVKFISISGEEPNPLY
                     TPLDISEIDFERDISFPGEYPYTRGVRTNMYRGRLWTMRQFSGMGTPIQTNERYHFLL
                     ERGQTGLSVAFDLPTLMGYDSDHPRSLGEVGKCGVAVDSLKDMEMIFNGISLDKISTS
                     MTINAPANILLAMYIVVGEKQKIPTEKLTGTLQNDILKEYIAQKEWIFPPEPSMRLIT
                     DNLEFCTNHMPKWNTISISGYHIREAGATAVQELAFTLADGFAYVEAGIKVGLDVDDF
                     APRLSFFFNSHLDFFEEIAKLRAARRIWARHMKERYKAKKEESTLLRFHTQTAGCSLT
                     AQQPENNIVRTAFQGLAGVLGGTQSLHTNSMDETLALPSEKAVQIALRTQQVIAYETG
                     VANTIDPLAGSYYIESLTNKMEEEAEKYFAEIEKRGGVLKGIEDGYFQREIAEAAYRY
                     QKEIEQKDRIIVGINDFTIAGEKVEIPLTVIDEKVQNEQINAVKEVKRNRDSDKVKRS
                     LEELRKVAKSGENTMPATIECVRCYTTEGEIVDVLREIFGEYQEPPLI"
     gene            7440..8135
                     /locus_tag="VGB16_04075"
     CDS             7440..8135
                     /locus_tag="VGB16_04075"
                     /inference="COORDINATES: protein motif:HMM:NF037887.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="Cthe_2314 family HEPN domain-containing protein"
                     /protein_id="HEX9916893.1"
                     /translation="MEYQEYQNRVKIRERIDKELPRLYFNLDNRISDPFNEEEINRSN
                     SAAWLQDIWLTYSYARWSLLEAFSYLELEYEVAFLYYSDNAVFRLHALPEKVVQMINC
                     YYGLEINIEDVTFHKFFRYCRGILKKLNPDLFKVLDDLKKNTQFQNLIKKYRHLKIHR
                     MEPYLKEMDNYRFLMKKVDSKRIDLIGDTGNFEWTIPKLREDLFKSYETIINFVTDTL
                     RLLFGEEVSVKET"
     gene            8152..8571
                     /locus_tag="VGB16_04080"
     CDS             8152..8571
                     /locus_tag="VGB16_04080"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_013178196.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="cobalamin B12-binding domain-containing protein"
                     /protein_id="HEX9916894.1"
                     /translation="MERKFRILLAKPGLDGHDRGVKVIASALRDAGMEVIYTGLRQTA
                     EVIVEAAIEEDVDAIGMSILSGAHMTLFPAVLKLLKEKGAEDILVFGGGIIPREDIEK
                     LEKLGVGKLFTPGATTIEAIEYLRKEIPKRKKKEKIF"
     gene            8596..9738
                     /locus_tag="VGB16_04085"
     CDS             8596..9738
                     /locus_tag="VGB16_04085"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_008442135.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016627 - oxidoreductase activity, acting
                     on the CH-CH group of donors [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="acyl-CoA dehydrogenase family protein"
                     /protein_id="HEX9916895.1"
                     /translation="MTYNIVESQQKVRDMAREFGKKEIAPIAKDLDRKPSEFPHELFK
                     KMAKAGFMAFPMKKEYGGLGKPKIDYATLIEELAWFDASAAVIMAVSGLAAYPIEAFA
                     SEEQKKKYLPKMISGEWIGAFALTEPDAGSDAANQKTTARVEGDYFIINGEKIFITSG
                     NVADIIILIAKIRDGEERDKVSAIIVEAKTPGITSEVLKWKMGIRASTTARLKFENVR
                     VPKTNLLGEAGKGFKMSLKTLDSARVGIAAQAVGIAQRVFDDSINYAKTREQFGAPIG
                     KLQAIQWMIADMSTRLEAARLLTYKAALMEDKGESFSREAAQAKLFASEAAHFCVDRA
                     MQIHGGYGFIGEFSEIERFYRDQRITEIYEGTSEVQRLVIAANYLR"
     gene            10202..11044
                     /locus_tag="VGB16_04090"
     CDS             10202..11044
                     /locus_tag="VGB16_04090"
                     /inference="COORDINATES: protein motif:HMM:NF012955.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="MBL fold metallo-hydrolase"
                     /protein_id="HEX9916896.1"
                     /translation="MDLGNLQIYSIVENSFKVDGGAMFGVVPKILWQKYISADKDNFI
                     TLDINLLLVKSKDKNILIDTGIGDALTKRQKEMFGIEKPSNLDGSLRDMSLSPEDIDI
                     VILSHLHADHSGGVIKLDKDGKKVPHFPNAKHYAQKREWEDAINPDERTSATYLTENP
                     MLLKENNLVELVDGEKEIIKGMKISSSHGHTPGHQIVFIDGGKEKIIYAADIIPTTAH
                     LRAAYVAGVDLYPRETMQAKKEIIKKCISESWMLALDHDVNTKLCRLRQEDGRVVFEK
                     VEFE"
     gene            11069..>11973
                     /locus_tag="VGB16_04095"
     CDS             11069..>11973
                     /locus_tag="VGB16_04095"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_014560295.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="carboxyl transferase domain-containing protein"
                     /protein_id="HEX9916897.1"
                     /translation="MSQEKNIEKLKKMREQAKLGGGQKRIEAQHKKDKLTARERIDLL
                     LDENSFEEFDMFVTHRCRDFGLEKESYLGDGVVTGYGTIDGRLTFVFSQDFTVFGGSL
                     SEAHAEKICKIMDMAMKVGAPVIGLNDSGGARIQEGVVSLGGYANIFLKNTLASGVIP
                     QISVILGPCAGGAVYSPAITDFIFMVKGISYMFVTGPNVVKTVTHEEVSYDELGGAAV
                     HSTKSGVAHFACENEAETLSLVRKLFSYVPLNNMEDPPEMECKDPFDRQDEELDLIVP
                     ENPNKPYDMKEIIKKVVDDGDFLEV"
BASE COUNT         3784 a         2094 c         3032 g         3063 t
ORIGIN      
        1 catgcggtaa tgattggtgg gcttttcgcc ggactagagg aaagtccggg agatagaatt
       61 gtcttcgagg ggaggagtta taaaattcat cggggtatgg gttctctgga agctatgaga
      121 agtggaggaa aagaccgtta ctttcaggaa catgaggctg aactcaaaaa gcttgttcca
      181 gagggagtag aggggcgagt gccatacaag ggaagtcttt ctgacgcggt gtatcagctg
      241 attggagggg tcagggctgg catgggattg tgtggagcaa gaaacgccga agagttgaga
      301 aagaaaggcg aattcattca gataacttat gccggtttga gggaaagtca tccgcacagc
      361 gtgattatta ccaaggaagc gcccaactat tgggtaagtt agaagacttg ttctttttca
      421 taaattattt cagatgcgat taacgcacaa aagccagagt ggcaattgca ttcgaataaa
      481 tttcgaatta gatttgccaa agcagacaag gcgatcgccc ccgcctttgg cgggggagga
      541 aagtccgagc tcctcaaggc aaagcgcctc gtaatgcgag ggttccgatt ttttagaatc
      601 gggaacggaa agtgccacag agaatagact atcccgcctg tggcgggata caggtgaaac
      661 ggcggtgtaa aagaccaccg ctccgataga gatatcggag gcacggaaaa ccccgcttgg
      721 agcaaggcca aatagggaag gagaagcagc ctgcttcgct attacttccg ggtaggccgc
      781 tggagttcga aggcgacttc ggacctagat tgatgatcgc cagtctcctg cctttggcgg
      841 gaggacacag aactcggctt attgtctgct ttggcatttt tgataaaggc cttcgcaaaa
      901 ctccctctcc ccttggggag agggtagggg tgagggggca atgaagcgga ataatatcgg
      961 acgatgcagg gcccttcgga agaatcaaac tgacgccgaa aggaagcttt ggtcaatatt
     1021 gagaaatcgt caacttggag gggcaaaatt taggaggcag tgttctgtag atagattcat
     1081 attagatttc tattgccctg aatgtaaagt tggaattgaa gctgatggcg gacaacacta
     1141 caaaaagtgt agtagaaagc aggatgaact cagaacgaaa gagttgaata aactgggtat
     1201 caagataatg agattcactg atcgggaaat attggttaat actgatggag tctacgagat
     1261 actcaagaca gccatagata aaaagagagc cggtccccct cacctttatc ctctccccaa
     1321 aggggagagg aggtaaggga attaaaaaag ttttcccact gtgtaaagta taaaaatggg
     1381 gtaaaactta aggaaatgga cacatgagaa cccacataaa tagacggttt gccggtggag
     1441 gaggcacaaa ataggagtat ctgcaagacc gcatgaataa aggtgtttca tcgttttgtg
     1501 caaggtgctt atttagtata aaaggacaca agaagtgaag ttatgcgttc tcttattgat
     1561 agtgctgatg tttgttttgt ccggatgcag tatctggtat gattttatgt ccgttgttgg
     1621 agatcatggt tatcgagttt gcgcttttat tagaggtgtg gtagtagata gctcaacaca
     1681 taaaccttta aaagatgttt ctgtcaaaat atatcgctat aaatatcttg agtaccagga
     1741 catcactgac agcgcaggaa tgtatggaca taataaagtt gaactattgt ttagttcaac
     1801 ttcaaaggag atatctgaac tgagaaagaa gcctcctata agaaaaaaga tagaactaag
     1861 aatggtcttc gaaaaaacag gctacagaaa acatgagctt ttagttccag ttgagtttgt
     1921 gtgttgctct cttcatcctc ctcaagaagt tactatatcc gaagtcaaaa ttgaggatgt
     1981 gtatttggtg aaggaatagc ttgaatggag aggttcagta tatggggatt attattcaag
     2041 caagtaatta aggagaaagc aatatgagtt taaaatggga attcgctcca aagccagagg
     2101 aaaaattggt agcagaaata agctcccagc ttgatttgaa tcccatagta acaaggatac
     2161 tggttagccg aaatatagat acatctgcca agatcaaaag atttttgaat tccagcctga
     2221 atgatttgtg cgacccatat cttctgggcc aaacggatat tgcggtggat aggataatcc
     2281 gcgctttgcg tgaaaatgag aagatgatga tattcggtga ttacgatgtg gatggtatta
     2341 ctgctacggc tttgatgttt ttggttttga atagattagg cggagaggtt tcgtattatc
     2401 ttcccaatcg ccttttggaa ggatacggtt tgtccgaaga tgggattctg gaggcagaac
     2461 agcgaggggt tagattaatc atcactgttg actgtggtgt caccgcttat gaagagataa
     2521 atttcgccag gcagaaaggt atagattgca tcatcactga ccatcatgag ccgggagaca
     2581 aacatctgga tgcagtggcg gtcataaatc cgaaacaaaa aggagaggat ggtctggcag
     2641 gcgagctttc tggagtggga gtggctttca agctggctca agccctgtac caaaagttgg
     2701 agcaagacca aacggagttg gaagaccatc tggatttagt ggctctggga actgcggcag
     2761 acattgttcc tttgacaggt gaaaatagga tactgacgaa gttcggaatc aagcagataa
     2821 ccaagaccac caagccggga ctgaaatcgt tgatctttgt ttccggtatt atgggaaagg
     2881 agataaacac cggtcaggta atgtttctct tggctccgag gataaatgcg gtgggaaggt
     2941 taggtgatgc ggaaaaggca atcaagcttt tgactactaa agacgaaaga ttagccgcag
     3001 agctggctcg gtttttggat gcggaaaaca aaaagagaaa agatatagat gaaggcactt
     3061 tagggcaggc attggagatg gttgaaaagg aggtcaatct ggatgaagat agggctattg
     3121 tcctttcttc taacaactgg catccgggag tgataggaat tgtggcttcg cgcatagtgg
     3181 agcggtatca tcgtccgacc gtaatgattt ccatagacaa agatgaaggg aaaggttcag
     3241 ccagaagcat tgcctctttt catctttatg atgctctaaa ggaatgcgat gatttacttc
     3301 taaaatatgg aggacacaaa tacgctgccg ggctttccat actacctgag aagataccgc
     3361 aattcaaaga gagattgaaa gaggtggctc atagaaagtt gaaagcagac gatttgattc
     3421 ccggtcttta tgtggatgct gaaattgaat tggagcagat agatgacaac ttatccgatt
     3481 tgttggaaaa gttttcaccc tttggtccgc agaataacag gccggtcttt gtcactcgaa
     3541 atctggaagt aatgggtcag ccctacgttg taggaaacaa ccatctcaag ctcaaagtga
     3601 gaaaaggcgg agtcgttttc gacgttatcg gttttggatt cggggattgg attaagcccc
     3661 tcagcatgac caaaattggt gtggatttag cttatgtggt agagaaagtg gaatggaacg
     3721 gagaaagtaa gacccagtta agaatcaaag atattaagtt ttctgggagt taggatgaat
     3781 gttctgaaga ggttttatcg aggcgaaaaa accgcgttag ccaagctgat atcctatgtg
     3841 gaaaatcagg aatcggacta tgaaaagata ttggctcaac tctatcctca agccaacaaa
     3901 gcctatcata ttggcataac cggtccccct ggagcaggta agagcacttt ggtagataaa
     3961 ctcactgcca tcctgcttaa agacaataag agaatcggaa taatcgcagt tgaccccacc
     4021 agtcctttta ccggcggtgc cctgttaggc gatagaatca ggatgcagga tttgactaat
     4081 aaagaagggg tcttcattcg cagtatggca actcgcggtt cttttggtgg tttagccaca
     4141 tccaccaagg aggtctccct gattttggat gcttttggca aagactatat catcatcgag
     4201 actgtaggcg taggtcaagt tgaattagat atagtgaatg cctgcgatac caccattgtg
     4261 gttttggtgc ccgaatcagg agatagcatt caagctatga aagccggctt gatggagata
     4321 gcagacatct tcgtggtgaa taaaagtgat agggaaggtt cggaaagaat tatctccgag
     4381 ctgaatatga tattagatat tcgtcgcaag aagggggaat gggaatatcc ggttgtgccg
     4441 actaaagcgg taaataatga agggatagga cagcttttag agagaataga atcccatagg
     4501 agatttatta ccggaaattc aatattcgag aagaatagaa aaaaacagat aaaaaacgag
     4561 ttggcaaaaa tcctggagaa taagctgaga aagcttctgg aaaaagatat cctgaaagat
     4621 ttcaatttgg accagatggc ggaggagatc tatcagggga aagacaatcc ttatgatata
     4681 ggcgaaaaga ttctaaagca ggtagatttt tcaaggtaaa ttggagattt gataaaatgt
     4741 tgtcaggaat agaaggtttg ttgctcttgt gttgggattg gaattgggca aattttgttg
     4801 tcaatctatt ggttggtgta gggactatag gagttgtgtt attagtcctt tatcaggaat
     4861 tctggagaac accaaagctg gaagtcgaat ttgatcaaaa taaggaagga ttctgtagat
     4921 atgcatcagt aaattgggta attaaacata tcccaaacag attagtatat tgcgagatag
     4981 accaatctgt attgacatat tgggttagaa ttaaagttat caatattggt aaaacagttg
     5041 ctaaaaatgt tgttggaaga gttaacgaaa tgttttgcat aaaagataat aaggtaactt
     5101 tttgtcagcc atttgatccc tgtatcttgc attgggttgg acagaaagga gacgacctca
     5161 atctgatcga cgttaataaa aaggaatatt actatttgga tattctgttt atagagaaag
     5221 acgacccttg tgtctacatt aatacagact tgaatcaaac gagaggtata aagtataatt
     5281 gggaagcagg agactatctg ctgaagatag ctttgtacag cgagaatgcc aaaccagtat
     5341 ctaaaacact tcgtgttgtc tggaatggca agtggaatga gatagaagtt tctttatcaa
     5401 gataaacacg catagggaag gagtttatgc agtttggcag ccttggattt tctttcttgt
     5461 cattcctgcg caggcaggaa tccagttttt aagattggat tcccgctttc gcgggaatga
     5521 cagcccgaca ggtgcgggtg aagataaacc ctacccctac gcgaaactca aaatgggaca
     5581 aggtgtccaa cctacgaaaa gttcgtccta actaaagtct ataacggagt gaggaaaatg
     5641 tatcaagaat cgttcaaaca aaaaattaga aagaaaaaag aagagtggga tgggaagttc
     5701 aaaaaatgca ggaaccgtga agaggtaaaa ttcatctcca tttccggaga agagccgaac
     5761 ccgctttata caccattgga tatctcagaa atcgattttg aaagagacat ttcctttccc
     5821 ggagagtatc cttacactcg cggcgtaagg acaaatatgt atcgaggtcg cttgtggact
     5881 atgcggcagt tctccggaat gggaacacca atacagacca atgaacgcta tcatttctta
     5941 ttagaaagag gacagaccgg actatccgta gcttttgatt tgccaacctt gatgggttac
     6001 gattctgacc atcctcgctc tctgggtgaa gtgggaaagt gtggggtggc ggttgattct
     6061 ttaaaagata tggagatgat tttcaatggc atctccttag ataaaatctc cacttcgatg
     6121 accatcaacg cacccgccaa tatacttttg gcaatgtaca tcgtagttgg cgaaaaacag
     6181 aaaatcccga cagagaaatt gaccggcact ttacaaaatg atattctcaa agaatatatt
     6241 gcccaaaagg agtggatttt cccacccgaa ccatcaatga gattgattac cgataacctg
     6301 gaattctgca ccaatcatat gcccaaatgg aacaccatct ccatttcggg ataccatatt
     6361 cgcgaagccg gagcaactgc agttcaagaa ctggctttca ctctggcaga tggctttgcc
     6421 tatgttgaag caggaataaa agtcggtttg gatgtagatg attttgctcc gaggctatcc
     6481 ttctttttca attcacattt ggatttcttt gaagagatag ccaaacttag ggcagccaga
     6541 aggatttggg caagacatat gaaggaacga tataaagcca aaaaagagga gtcaactctt
     6601 ttgagatttc atactcagac cgcaggttgc agcttgacag cccaacagcc ggaaaacaat
     6661 atcgtccgca cagcgtttca gggtctggct ggcgttttag gcggaactca atccctacac
     6721 actaactcta tggatgagac cctggcattg ccatctgaaa aagcagttca aatagctttg
     6781 cgtactcagc aagtgattgc ttacgagacc ggcgtggcta ataccattga ccctttggca
     6841 ggctcctatt atattgaatc tttgaccaat aagatggaag aagaagccga gaaatacttt
     6901 gctgaaatag agaaaagagg cggggtattg aaaggtattg aggatggata ttttcaaagg
     6961 gagatagcgg aggcggctta ccgctatcaa aaggaaatag aacagaaaga cagaattatt
     7021 gtgggaatta atgattttac tattgccgga gagaaagtgg aaatcccctt gacggtgatt
     7081 gatgagaagg ttcagaacga gcagattaat gcagttaaag aagtaaaaag aaatagggac
     7141 agcgataaag ttaaacgttc cttggaggaa ttgagaaaag tcgctaagtc aggtgagaat
     7201 acaatgcctg ctacaattga gtgcgttcgg tgttatacta cggaagggga gatagtggat
     7261 gtgctaagag agatttttgg agaatatcaa gagccacctt tgatttgata aattcatatt
     7321 taggtatttg ctgtcattgc gaggattccc gtagggagac gaagcaatct gtttagattg
     7381 cttcgctgcc gctcgcaatg acaaacaagt catttcgcct acggagtgaa aaactaatta
     7441 tggaatatca ggagtatcaa aacagagtta aaatcagaga aaggattgat aaagaattac
     7501 ctcgactcta ttttaaccta gataatcgca taagtgatcc atttaatgaa gaggaaatta
     7561 accgttccaa ttcagcagct tggcttcaag atatttggtt gacatattcc tatgctagat
     7621 ggagtctgct agaagctttt tcgtatctag aattggagta tgaagtagca tttctttatt
     7681 acagcgataa tgcggtcttt agactgcatg cacttccaga aaaagttgtt caaatgatca
     7741 actgctatta tggtttggaa attaacatag aagatgttac ttttcataag ttcttccgtt
     7801 attgtcgagg aatactcaag aagctcaatc ctgacttgtt taaggttctg gatgatttga
     7861 agaaaaatac ccagtttcaa aacctaatta agaagtatag acacctaaag attcatcgta
     7921 tggagcccta tttaaaagaa atggacaatt atagattttt aatgaaaaag gttgattcta
     7981 agagaattga tttaatcgga gataccggga attttgagtg gactattcct aaactcagag
     8041 aagatttgtt caaatcatat gaaacaataa tcaattttgt aacagatacg cttcgtttat
     8101 tgtttggaga agaggtttcg gttaaggaaa cataactcaa agaggtaacc catggaacgg
     8161 aaattcagaa tccttttagc caagcccggt ttagatgggc atgacagagg agtcaaggtc
     8221 atcgcttcag ccctgaggga tgctggtatg gaagtgatat acacagggtt acggcaaaca
     8281 gctgaagtaa tagttgaagc ggcaatcgag gaggatgtgg atgctattgg gatgagcatt
     8341 ctctccggag cccatatgac tctcttcccg gcagtgctta aactcttaaa agaaaaaggt
     8401 gccgaagata tccttgtttt tggcggaggg ataatcccca gagaggatat tgagaaattg
     8461 gagaagttgg gagtgggtaa actctttact cctggcgcca cgactattga agcaattgag
     8521 tatttaagaa aagaaattcc aaaaagaaag aaaaaagaaa agatttttta actattaaac
     8581 ctaaaggagg cgagcgtgac ttacaacata gtggagagtc agcaaaaagt tcgggatatg
     8641 gcaagggagt ttggcaaaaa ggagattgct cccattgcca aggatttgga ccggaaacca
     8701 tcggagtttc cacacgagct tttcaagaag atggcaaaag ctggctttat ggcatttcct
     8761 atgaaaaagg aatatggtgg cttgggaaag cccaagatag attatgccac attaatcgaa
     8821 gaattggctt ggtttgatgc ttcagccgca gtgataatgg cagtgagcgg cttagccgcc
     8881 tatcctattg aagcttttgc cagcgaagag cagaagaaaa aatatttgcc caagatgata
     8941 tccggtgaat ggattggagc tttcgccttg actgagccag atgccggctc cgatgctgcc
     9001 aatcaaaaaa ccactgccag agtggaaggg gattatttta tcatcaacgg tgaaaaaata
     9061 ttcatcacca gcggaaacgt agcagacatt ataatcttga ttgctaaaat acgagacggc
     9121 gaagaaagag acaaagtatc agccattata gtggaagcca aaactccggg cataacttca
     9181 gaggttttga aatggaagat gggtatccgg gcttcaacca cagccagact gaagtttgaa
     9241 aacgttcgcg ttcccaaaac caatctttta ggagaggcgg gaaaaggctt caagatgtct
     9301 ttgaagactc tggatagcgc ccgtgtcgga attgccgctc aggcagtagg aattgcccaa
     9361 agggtatttg atgattccat aaactatgcc aagaccagag agcagttcgg tgctccgatc
     9421 ggtaagcttc aggcaattca atggatgatt gctgatatga gcacaagatt agaagcggca
     9481 cggcttttga cttacaaagc ggcattgatg gaagacaaag gggaaagctt ctccagagaa
     9541 gcggctcagg caaagctttt tgcttcggag gcagctcact tctgtgttga tagggcaatg
     9601 cagattcatg gaggatatgg tttcatcggt gagttctcag aaatagagag attttatcgc
     9661 gaccagagaa taaccgaaat ttacgaagga acatccgagg ttcaaaggtt agttatagcg
     9721 gctaattatt tgagataaaa cacggacggg cttaaaagtc cgcctctaca tttactgttt
     9781 ttgtcattgc gagaaatgag aaacgaatgg cgaagcaatc tattgtagga tgaaaagttg
     9841 agattgcttc gcaataacga ataagcaaat cacaattcgc aggtctattg tttgtggggg
     9901 aattgaattt cccacacaaa agatgagttc agggattcca attcccgaac taactaaaac
     9961 agaacttttt caattgtcat tcctgcgaaa gccagcggcc ttgcccacgg cttcgccgtg
    10021 aggcgagccg ctaggcaggc aggaatccag aagagaaaaa caagtgtgga ttcctgatta
    10081 aaccctcggg aatgacaaaa cataaatcaa aattcgtagg tcgtgcatcc ctggccgtgg
    10141 gttaagcggg gagaaatggg caggcctgcc gaccttgcga ggcaagttag attttaaaaa
    10201 tatggattta ggaaatctgc aaatctattc tatcgttgaa aatagtttca aggtggatgg
    10261 cggagcgatg tttggcgtgg tgccaaaaat cctgtggcaa aaatatattt ccgcggataa
    10321 ggacaatttc ataactttgg acatcaatct tctgctcgtt aaatcgaaag ataaaaatat
    10381 cctaatcgat accggaattg gcgatgccct gaccaaaaga caaaaagaga tgttcggaat
    10441 tgaaaaacca tccaatttag atggaagttt gagggatatg agtctatctc cagaggatat
    10501 agatattgtt atcctgagcc atctgcatgc tgaccattca ggtggggtaa taaagctgga
    10561 taaagatggc aagaaagttc cccattttcc aaatgctaaa cactatgccc aaaaaagaga
    10621 atgggaggat gccataaacc cggacgagag aacatctgcc acctatttga ccgaaaatcc
    10681 gatgttattg aaagagaata accttgtgga attggtggat ggagaaaaag aaataattaa
    10741 gggaatgaaa atctcaagct ctcatgggca cacccccgga catcaaattg ttttcattga
    10801 tggtggcaaa gaaaaaatca tctatgccgc agatataatt ccaaccaccg ctcacctccg
    10861 ggcggcttac gttgcaggag ttgaccttta tccgagggaa acaatgcaag caaaaaaaga
    10921 gattattaaa aaatgcataa gtgaaagttg gatgctggcc ttagatcatg atgttaatac
    10981 taaactctgt cgcttgaggc aagaggatgg aagagtggtc tttgagaaag tagagttcga
    11041 ataagaaaaa ctttggcgga aggattttat gtcgcaagaa aagaatatcg aaaaactgaa
    11101 gaagatgcgc gagcaagcca agctgggagg tgggcagaag aggattgaag ctcagcataa
    11161 gaaagataag ctcaccgcca gggaaagaat cgatttactt ttggatgaaa atagcttcga
    11221 ggaattcgat atgttcgtca ctcaccgctg tcgagatttt ggtttggaaa aggaaagtta
    11281 tttgggggat ggggtggtga ccggatacgg aaccatcgac ggacgattaa cttttgtctt
    11341 ttcccaagat ttcaccgttt tcggcggctc gctttccgaa gcccatgcag agaaaatttg
    11401 caagattatg gatatggcaa tgaaggttgg agcaccggtg attggcttaa acgattcagg
    11461 gggtgcgaga attcaggagg gcgtggtgag cttgggcggg tatgcgaaca ttttcctaaa
    11521 gaacacgctt gcttccggag tcattcctca aatttcagtt atccttggac cttgtgctgg
    11581 tggagcagtc tattctccag ccataaccga tttcatcttt atggtcaaag ggataagtta
    11641 tatgttcgtc accggtccga acgtagtcaa aaccgtaact cacgaggagg tgagctacga
    11701 tgagttaggc ggggcagcag ttcacagtac caaaagcgga gtggcgcatt ttgcctgtga
    11761 gaatgaggct gaaactttat ccttagtcag aaaactcttt agctacgtcc cgttaaacaa
    11821 tatggaagat ccaccggaaa tggagtgcaa ggacccgttt gacaggcaag acgaagagct
    11881 ggacctcatc gtaccagaga atccaaacaa gccctatgat atgaaggaga taataaagaa
    11941 agttgtggat gacggagatt tcttggaggt aca
//
LOCUS       DASWLN010000123         4228 bp    DNA     linear   ENV 20-FEB-2024
DEFINITION  MAG TPA_asm: Candidatus Zixiibacteriota bacterium isolate
            SMAG_U2949 k141_486551, whole genome shotgun sequence.
ACCESSION   DASWLN010000123 DASWLN010000000
VERSION     DASWLN010000123.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37960594
            Sequence Read Archive: SRR10376091
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Candidatus Zixiibacteriota bacterium (soil metagenome)
  ORGANISM  Candidatus Zixiibacteriota bacterium
            Bacteria.
REFERENCE   1  (bases 1 to 4228)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 4228)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (26-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/12/2024 08:28:26
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 1,440
            CDSs (total)                      :: 1,416
            Genes (coding)                    :: 1,412
            CDSs (with protein)               :: 1,412
            Genes (RNA)                       :: 24
            rRNAs                             :: 1 (16S)
            partial rRNAs                     :: 1 (16S)
            tRNAs                             :: 21
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 4
            CDSs (without protein)            :: 4
            Pseudo Genes (ambiguous residues) :: 0 of 4
            Pseudo Genes (frameshifted)       :: 0 of 4
            Pseudo Genes (incomplete)         :: 3 of 4
            Pseudo Genes (internal stop)      :: 1 of 4
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..4228
                     /organism="Candidatus Zixiibacteriota bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_486551"
                     /isolate="SMAG_U2949"
                     /isolation_source="soil"
                     /db_xref="taxon:2053527"
                     /environmental_sample
                     /country="USA: Oregon"
                     /lat_lon="42.99 N 123.63 W"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            <1..648
                     /locus_tag="VGB16_04100"
     CDS             <1..648
                     /locus_tag="VGB16_04100"
                     /EC_number="1.3.1.14"
                     /inference="COORDINATES: protein motif:HMM:NF005574.0"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004152 - dihydroorotate dehydrogenase
                     activity [Evidence IEA];
                     GO_process: GO:0006221 - pyrimidine nucleotide
                     biosynthetic process [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="dihydroorotate dehydrogenase"
                     /protein_id="HEX9916898.1"
                     /translation="KTKLIVNVAGSTVEEYIEVVEKLNQSRGIDMLEINISCPNVKEG
                     GMIFGCDAGIAYDCVKKVKKKSRFPIMVKLSPNVKDITEIGLAVQDAGADAISLINTL
                     VGMAIALEKRKPILSNVTGGLSGPAIKPVALAMVYKVAQKVKIPVIGIGGILDCRDAL
                     EFILAGASLVQIGTASFVDPQSSIKIIEGIEKYCRENNISSSRSLVGKLDTSKKE"
     gene            652..1488
                     /gene="pyrF"
                     /locus_tag="VGB16_04105"
     CDS             652..1488
                     /gene="pyrF"
                     /locus_tag="VGB16_04105"
                     /EC_number="4.1.1.23"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_007905285.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004590 - orotidine-5'-phosphate
                     decarboxylase activity [Evidence IEA];
                     GO_process: GO:0009220 - pyrimidine ribonucleotide
                     biosynthetic process [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="orotidine-5'-phosphate decarboxylase"
                     /protein_id="HEX9916899.1"
                     /translation="MKFTEKLLNISSKNNSLVCVGLDADLNKVPKLLLKEKDVIFEFN
                     KKIIDATSDLVCAYKPNMAFYELYGNKGWESLQKTCQYIPDEIPIIIDAKRGDIGNTA
                     RMYAKAFFEELKADAITLSPYMGQDAISPFLEYKDKCALVLCLTSNIGAEDFQLLEMR
                     DEPLYIKVAKKVVSWNKNKNCGLVVGATQAEQLRGIREIAPDMPILIPGIGAQGGDLE
                     MAVKFGTDKNGNLAIVNSSRSILYASSGEDFAQKAKEETEKLREEINLHREKMMSDSE
                     PV"
     gene            1688..3226
                     /locus_tag="VGB16_04110"
     CDS             1688..3226
                     /locus_tag="VGB16_04110"
                     /inference="COORDINATES: protein motif:HMM:TIGR00196.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0052855 - ADP-dependent NAD(P)H-hydrate
                     dehydratase activity [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="NAD(P)H-hydrate dehydratase"
                     /protein_id="HEX9916900.1"
                     /translation="MKLVTAREMREIDKETIEGVGIAGLELMEKAGRETAEVAREMLI
                     NPKGKKVAIFCGRGNNGGDGFVIGRYLSGWKAKVNFYLLSKISEVKGDAKLNLNKAKK
                     LKLPIKEITKKQHLPKRIEADLIVDAIFGTGFESEISGLAGDMVDLMNSSNIPVLSVD
                     IPSGVNAATGNLSKICIQAEKTVTMGLPKIGQFVFPGKKYCGEVRMADIGIPDSVIKN
                     KKIDLNLITAKEIKALLPKRKPYAYKGDCGRVVLIAGSTGMTGAACLAAGSSLRAGAG
                     MAILGIPESLNSIFEIKLTEVMTKPLPDVRKRGALALRGLGEIKQLLKWGNCVALGPG
                     LGQHFETIELIKRLIQQIELPTVVDADGLNSIAKDISILKNCKAPFILTPHIGELSRL
                     NKVPIEEIAKDRIKYAKEFAREYNCVLVFKGAPTIIAEPKGQVYINPTGNAGMATAGS
                     GDVLTGIIVGFLAQGLSTLDSAICGVYIHGLAGDLACKEKGQMGMIAGDIMRQIPEAI
                     LKIN"
     gene            3505..>4228
                     /locus_tag="VGB16_04115"
     CDS             3505..>4228
                     /locus_tag="VGB16_04115"
                     /inference="COORDINATES: protein motif:HMM:TIGR01891.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004180 - carboxypeptidase activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="amidohydrolase"
                     /protein_id="HEX9916901.1"
                     /translation="MNKQNYIQIKEISEEIFPYLVKLRRRFHQYPETAFEEFKTSQMI
                     AKELRKIGLRVKTGVAKTGVVGLLESGKIGKTVALRADMDALPITELTNLPYASKNKG
                     KMHACGHDAHITCLIGAAMILNKLKDNFKGSVKFIFQPSEEVSPGGAIQMIKEGVLLN
                     PKVNAIFALHCDSNIPVGKVGVKNGIMMAYADDFDIIITGKSGHGARPHDGVDAIVIA
                     SQIINSLQTIVSRRIDPLRSAVV"
BASE COUNT         1378 a          735 c         1034 g         1081 t
ORIGIN      
        1 aagaccaaac taatagtcaa cgttgcggga agcacagttg aggaatatat agaggtcgtg
       61 gaaaaactaa accaatcccg ggggattgat atgttagaaa taaatatctc ctgccctaat
      121 gtaaaagaag gtgggatgat atttgggtgc gatgccggga tagcttacga ctgcgtcaaa
      181 aaagtaaaga agaaaagcag atttcccata atggtcaagc tttcccctaa tgtcaaggac
      241 ataactgaaa ttggtctggc agtccaagat gcgggtgcgg atgccatttc attaattaac
      301 accttagttg gaatggcgat tgccttggaa aaaagaaaac caattctttc aaatgttacc
      361 ggtgggcttt ccggtccagc cataaagccg gtggctttgg cgatggttta taaagtggca
      421 cagaaggtca aaatcccagt cattggtata ggcgggatat tagattgcag ggatgcttta
      481 gagtttatct tagcaggtgc aagtctggtc caaattggaa ccgcaagttt tgttgacccg
      541 caaagttcaa tcaagataat cgaaggaata gaaaaatatt gcagagagaa taacataagc
      601 tcaagtagga gtctggtggg gaaattagat acaagcaaaa aggagtgaat tttgaaattc
      661 acagaaaagc ttcttaacat ctcttcaaaa aataattcct tagtgtgcgt tgggttagat
      721 gctgacttaa acaaagttcc taagttactg ctaaaagaga aagatgtaat cttcgaattc
      781 aacaaaaaga taatcgatgc cacttccgat ttggtttgtg cttataagcc gaatatggct
      841 ttttacgaac tgtatggaaa caaagggtgg gagagcttac agaaaacttg ccagtatatt
      901 cccgatgaga ttcccatcat aatcgacgcc aaaagagggg atatcggaaa cactgccagg
      961 atgtacgcta aggcgttttt cgaggagtta aaagctgatg ccataaccct gagtccctat
     1021 atggggcagg atgcgatttc gccgtttttg gaatataagg ataaatgcgc ccttgttctc
     1081 tgtctaacct ccaatatcgg cgcggaggat ttccagcttt tggaaatgcg agacgagccc
     1141 ctgtacataa aagttgccaa gaaagttgtt tcgtggaata aaaataagaa ttgtgggttg
     1201 gtagttgggg caactcaagc tgagcaactt agaggcataa gggagatagc gccggatatg
     1261 cccatactga ttccgggcat aggggcacag ggaggggatt tggaaatggc agtgaagttc
     1321 ggaacagata aaaatggaaa tttagctata gtcaattctt cccgctcgat tctctatgct
     1381 tcaagtggag aagatttcgc ccaaaaggcg aaagaagaga cggagaaact aagagaagag
     1441 attaatcttc atagagaaaa aatgatgtcg gatagtgaac ctgtgtagcg gtcgggcttg
     1501 gtgcggtctg taaataaatc cccctttatc cccctttgcc aaagggggat ttatggaaag
     1561 agttttcatc ttcccctctt ttacaaagag gggcaagggg agatttgaat tacagatatt
     1621 ttttatggat gttatacctg gctaggggca ggcaagcaat tacaaattct taaggttaag
     1681 gatgtttatg aagttagtta ctgccagaga gatgagagaa atcgataaag agaccatcga
     1741 aggggtgggg attgctggct tggagctgat ggaaaaggca ggcagggaaa cagcagaagt
     1801 tgctagagag atgctaatca atcccaaagg gaaaaaagtt gccatcttct gtggcagggg
     1861 aaacaatggt ggggatggat ttgtaattgg aagatactta tccggctgga aagcaaaggt
     1921 aaatttctat cttctatcca agatatccga agtcaaaggt gatgctaaac ttaacctaaa
     1981 caaagccaaa aaattgaaat tgcccataaa agagattaca aaaaaacagc atcttcctaa
     2041 aagaatcgaa gcggatttaa ttgtggatgc gattttcgga accggatttg aaagtgagat
     2101 ttccggtttg gctggggata tggttgattt gatgaattcc tccaatattc cagtgctctc
     2161 agttgatatc ccttcgggag tcaatgccgc cacgggcaac ctttccaaaa tttgcatcca
     2221 ggcagaaaag acagtaacta tggggcttcc caaaattggg cagtttgtct ttccgggtaa
     2281 aaaatattgc ggagaggtta gaatggctga tattggcatt ccggattcgg ttataaaaaa
     2341 caaaaaaata gatttgaacc ttatcactgc aaaagagata aaagctctac ttccgaaacg
     2401 taagccgtat gcttacaaag gggattgtgg tagagtagtc ctaattgccg gctcaactgg
     2461 catgactggt gcggcttgcc tggcagccgg atcatctttg agagctggtg ctggaatggc
     2521 aattttggga atccccgaaa gcttaaattc catctttgag ataaaattaa ctgaagttat
     2581 gaccaagccc ttgccagacg tgagaaaaag aggggccttg gctttaaggg gtttaggcga
     2641 aataaaacag cttctaaaat ggggaaattg cgttgcttta ggtcccggct taggacagca
     2701 ttttgaaaca attgaattga ttaagagact aattcagcaa atcgagcttc ctacagtagt
     2761 agatgcagat ggtttaaatt caattgccaa ggacatatcc attctaaaaa actgtaaagc
     2821 cccttttatc ttaacccctc atattggaga gctctcgcgc ttgaataaag ttcccataga
     2881 ggaaattgcc aaagacagga taaaatatgc caaggagttt gccagagagt ataattgcgt
     2941 tttggttttc aagggagctc cgactataat tgctgaaccc aagggtcagg tttatataaa
     3001 ccccactgga aatgctggaa tggcaacagc tggaagtggt gatgttttaa ctggaataat
     3061 agtcggtttt ttagctcaag gtttgtcaac tctggattct gcaatttgtg gtgtttatat
     3121 tcacggtttg gcaggggatt tggcttgtaa ggaaaaaggg cagatgggga tgattgctgg
     3181 ggatataatg cgacaaattc cagaggcgat tttgaaaata aattgaaaat taaaaaagaa
     3241 gaatgaaaaa ttaaaaaatc gatgtcggat gacgaatagc ggatatcgaa tagaaaaaaa
     3301 tgtaggggcg aatttttaaa tccgcccgag atgagattgc ttcgtctccc gcaatcgggt
     3361 aagtaatggc tgctgccatt actttcgggg aacagtgcaa ccgttcccta cccgctataa
     3421 aaaacgttca aaggagaaat agaatatgaa ggcgacccat atgggtcgcc ttcaaacgag
     3481 aacattttcg ggaataaata tcaaatgaac aaacaaaatt atattcaaat caaagaaatc
     3541 tccgaagaga tttttcccta tttggtaaag ctccgcagaa gattccatca atatccggag
     3601 accgcatttg aagaattcaa aacctcgcag atgattgcca aagaattaag aaaaatcggc
     3661 ttaagagtaa aaacaggagt tgctaaaact ggagtagtcg gacttttgga atcaggcaaa
     3721 attggaaaaa ccgtggctct gcgtgcggat atggatgctt tgcccatcac agagctgacc
     3781 aatttaccct atgcctctaa aaacaaaggc aaaatgcatg cttgcgggca cgatgcccat
     3841 atcacctgtc ttatcggtgc ggcaatgatt ttaaataaat taaaagataa ttttaaagga
     3901 agtgtcaaat tcattttcca gccgagtgaa gaggtttccc cgggcggggc aatacagatg
     3961 ataaaagaag gggtactttt aaatccaaaa gtcaacgcaa tttttgcttt acattgcgat
     4021 tcgaatatac cagtgggaaa agttggagtc aaaaacggga taatgatggc ttatgcagat
     4081 gatttcgaca taatcataac aggcaaaagt gggcatggag caaggccaca cgatggggtg
     4141 gatgccatag tgatagccag ccaaatcata aattcattac aaaccatagt cagccgaagg
     4201 atagacccct tgaggtctgc agtggtaa
//
LOCUS       DASWLN010000124        13614 bp    DNA     linear   ENV 20-FEB-2024
DEFINITION  MAG TPA_asm: Candidatus Zixiibacteriota bacterium isolate
            SMAG_U2949 k141_184736, whole genome shotgun sequence.
ACCESSION   DASWLN010000124 DASWLN010000000
VERSION     DASWLN010000124.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37960594
            Sequence Read Archive: SRR10376091
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Candidatus Zixiibacteriota bacterium (soil metagenome)
  ORGANISM  Candidatus Zixiibacteriota bacterium
            Bacteria.
REFERENCE   1  (bases 1 to 13614)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 13614)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (26-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/12/2024 08:28:26
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 1,440
            CDSs (total)                      :: 1,416
            Genes (coding)                    :: 1,412
            CDSs (with protein)               :: 1,412
            Genes (RNA)                       :: 24
            rRNAs                             :: 1 (16S)
            partial rRNAs                     :: 1 (16S)
            tRNAs                             :: 21
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 4
            CDSs (without protein)            :: 4
            Pseudo Genes (ambiguous residues) :: 0 of 4
            Pseudo Genes (frameshifted)       :: 0 of 4
            Pseudo Genes (incomplete)         :: 3 of 4
            Pseudo Genes (internal stop)      :: 1 of 4
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..13614
                     /organism="Candidatus Zixiibacteriota bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_184736"
                     /isolate="SMAG_U2949"
                     /isolation_source="soil"
                     /db_xref="taxon:2053527"
                     /environmental_sample
                     /country="USA: Oregon"
                     /lat_lon="42.99 N 123.63 W"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            complement(<1..195)
                     /locus_tag="VGB16_04120"
     CDS             complement(<1..195)
                     /locus_tag="VGB16_04120"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEX9916902.1"
                     /translation="MLRPSALHFVLSLSVEGRLRLEEMVNLSFDFAQDSEDVRLSGST
                     VPSGSPSKDSVEALVELLNHI"
     gene            299..769
                     /locus_tag="VGB16_04125"
     CDS             299..769
                     /locus_tag="VGB16_04125"
                     /inference="COORDINATES: protein motif:HMM:NF012549.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="NADH-quinone oxidoreductase subunit C"
                     /protein_id="HEX9916903.1"
                     /translation="MTIEEITQILNNKFPEKLKIIEVTSHQPAFFIEKEILLEICKFA
                     KQSDQLKIDFVNAIFAVDRTDSLEIVYVLFSLEKRHRLCLKAKLPRKDAEIDSVTQVW
                     EAANWFERETSELFGIKFKGHPDPRPLLLAEDWSEGYPMLRDWEGKDFIKLPPK"
     gene            773..1879
                     /locus_tag="VGB16_04130"
     CDS             773..1879
                     /locus_tag="VGB16_04130"
                     /EC_number="1.6.5.9"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_018085613.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="NADH-quinone oxidoreductase subunit D"
                     /protein_id="HEX9916904.1"
                     /translation="MPEEIHAEEFMVNMGPQHPSTHGVLRLMLTMDGEVVLDAIPHVG
                     YLHRALEKIAENRTFLQYIPYTDRLDYVSAMFGNYAFVYPIEKLANVVVPERAEYIRV
                     IMMELNRIASHLIWLGTFSLDLGAITPFLYCFREREQILDLFEMVCGQRMTFNYMRVG
                     GVSKDLPDEFVPMARKFVDYFKPRVDEYEEILTHNPIFLGRTKGIGLLDKETAVEHSV
                     SGPSLRGCGINWDLRKNEPYSIYDRFTFEIPLGKASDVWDRYEVRIQEMRQANLIVEQ
                     ALKQLPQGEFKTKIPAVFKPPAGEVYTRIEAPRGEMGFYIITDGTTKPYRVKIRTGSF
                     CNLTVLPKLCKGWKIADLVAIFGSLDVVLPEVDR"
     gene            1977..3005
                     /gene="nuoH"
                     /locus_tag="VGB16_04135"
     CDS             1977..3005
                     /gene="nuoH"
                     /locus_tag="VGB16_04135"
                     /EC_number="1.6.5.9"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_009171693.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0016020 - membrane [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="NADH-quinone oxidoreductase subunit NuoH"
                     /protein_id="HEX9916905.1"
                     /translation="MLELDKLIEAIYFWLEKIFVDLGIPKFFLDLLSMLLVALVIIIF
                     AALSVMFLVWWERKISGHFQSRFGPMRVGWHGWLQTIVDAIKLIMKEDIVPTSADKVV
                     FYIAPIILFISALLAYIVIPFGKGLVVRDLNIGILYILAITTFTVIGILSAGWSSNNK
                     YSLLGGFRSAAQIISYEVPLTLSVLGVVLLAGSMSLVTIVKSQNGIVHWFIWKQPLGF
                     LIYLTAAIAEVNRVPFDIPEAEQEIVAGYNVEYSGMKFAMFFFAEYMNTFLICAIATT
                     LFLGGWNGPILPSYVWFFIKTFFLIFCLMWLRWTYPRVRVDQLMDFAWKFLLPLAFLN
                     LIVTALLA"
     gene            3012..3455
                     /locus_tag="VGB16_04140"
     CDS             3012..3455
                     /locus_tag="VGB16_04140"
                     /EC_number="7.1.1.-"
                     /inference="COORDINATES: protein motif:HMM:TIGR01971.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0016020 - membrane [Evidence IEA];
                     GO_function: GO:0016651 - oxidoreductase activity, acting
                     on NAD(P)H [Evidence IEA];
                     GO_function: GO:0051539 - 4 iron, 4 sulfur cluster binding
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="NADH-quinone oxidoreductase subunit I"
                     /protein_id="HEX9916906.1"
                     /translation="MSILKKIIDGIKNLILGLGVTVKYLLKPAVTLQYPTERWTMPER
                     SRGMVSLLTDKEAKRLRCTGCSLCVRICPNNAIQMVPKKDEQGKRMPDTFVVDVGMCI
                     YCGLCQEVCPFNAIRLVPKYEFSVYDKKSLSFDKEKLAEIGKDYE"
     gene            3463..3960
                     /locus_tag="VGB16_04145"
     CDS             3463..3960
                     /locus_tag="VGB16_04145"
                     /EC_number="1.6.5.9"
                     /inference="COORDINATES: protein motif:HMM:NF012709.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008137 - NADH dehydrogenase (ubiquinone)
                     activity [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="NADH-quinone oxidoreductase subunit J"
                     /protein_id="HEX9916907.1"
                     /translation="MVSGTQIAAFYILAGIIICSSVMVVTMKNIFHSALFLVLCFFSV
                     AGIYILLEAEFVAAVQVLIYVGAITVLMIFAIMLTSQIYSPSIKQSNEQVIPALLIVV
                     ALLIVTLVSLAKTTWRINTALPTEQSTVTIGKSLLTVYVLPFEVVSVVLLVALVGAIV
                     IAKKE"
     gene            3964..4272
                     /gene="nuoK"
                     /locus_tag="VGB16_04150"
     CDS             3964..4272
                     /gene="nuoK"
                     /locus_tag="VGB16_04150"
                     /EC_number="1.6.5.9"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_007937465.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016651 - oxidoreductase activity, acting
                     on NAD(P)H [Evidence IEA];
                     GO_process: GO:0042773 - ATP synthesis coupled electron
                     transport [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="NADH-quinone oxidoreductase subunit NuoK"
                     /protein_id="HEX9916908.1"
                     /translation="MLGLIHYLTLAAILFCVGIFGVMTRRNAIGVLMSLELMFNAVNI
                     NFIAFSKFITPTILTGQIFSIFVITIAAAEAAVGLAIVILIYRNYKGINVDKINFMKW
                     "
     gene            4439..6361
                     /gene="nuoL"
                     /locus_tag="VGB16_04155"
     CDS             4439..6361
                     /gene="nuoL"
                     /locus_tag="VGB16_04155"
                     /EC_number="7.1.1.2"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011392500.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008137 - NADH dehydrogenase (ubiquinone)
                     activity [Evidence IEA];
                     GO_process: GO:0042773 - ATP synthesis coupled electron
                     transport [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="NADH-quinone oxidoreductase subunit L"
                     /protein_id="HEX9916909.1"
                     /translation="MLAYSWIIPFLPLLAFASIVLVTNPDKKLSSFLSIGAIVTSFLL
                     SIVVLIEVIASPVSKEFSISWLNIAGFKVDFGMLIDPLTSVMLMVVTIVSMLVQIYSV
                     GYMAGDPRFSRYFAFLSLFTFSMLGLVLSNNFFSIFIFWELVGLTSYLLIGFWFEKKT
                     ASDAGKKAFITTRTGDLGFIIGILILTTAAGTFNFQSIFGKIEAGTIPAGLVTLSAIF
                     IFAGAVGKSAQFPLHVWLPDAMEGPTPVSALIHAATMVAAGVYLVARCMGVYAASESA
                     GLVVAIIGVLTAFMAASIGLVQNDIKRVLAYSTISQLGYMIMALGLGGLTAGTFHLMT
                     HAFFKALLFLGAGSVIHAIHTNNIWEMGGLYKKMKITAITFIIASLSISGIFPLSGFW
                     SKDEILAVASEKHPLFLIIAALVAGMTAFYMFRLCFVTFFGKPKDQEKFHHAHESPKS
                     MTIPLCILAFLSIFAGWVGIPWLPGGYSFFVFYHHPHHAEPNYILMVVSTLVALSGIG
                     LAYLVYYKGTFSPDRLRERFKPIHKVLYNKYYVDEFYNMTIVNPILKLSDILFKFDLG
                     VIDWLVNGVGNFTVFLSNVKEWFDSHIVDGAVNGVGYVIKGIGSATRKIQTGQLQNYA
                     FIIFFAIVIILLIKLG"
     gene            6537..8006
                     /locus_tag="VGB16_04160"
     CDS             6537..8006
                     /locus_tag="VGB16_04160"
                     /EC_number="1.6.5.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_014857030.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016655 - oxidoreductase activity, acting
                     on NAD(P)H, quinone or similar compound as acceptor
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="NADH-quinone oxidoreductase subunit M"
                     /protein_id="HEX9916910.1"
                     /translation="MNFPILTYMTFIPLLGMIVILFINKEKIQLVRWTAALFSFVPLL
                     LSFILLSRFNSSTPEMQFMEKLEWIPSLGVSYIMGADGISVPMLFLTALLSFVSVIAS
                     FNIDKRVKEYFAFFLLLETGMMGVFSALDFFLFYIFWEVMLVPMYFLIGIWGGPRREY
                     AAIKFFLYTLFGSLFMLVGILALYFTVEPHTLNMMELIKKAPDYARGFQLLSFVAFYI
                     AFAIKVPVFPFHTWLPDAHVEAPTAVSVILAGILLKMGIYGLLRISLPMFPYGAIYFA
                     LPLAILGLVNIVYGALVSMAQKDLKKLVAYSSVSHMGYCILGISALTVTGLSGCVFQM
                     FSHGLITGALFLLVGVLYDRAHTRDIDAFGGLWVKLPVYSALMILFCMGSLGLPGLSG
                     FVSEFMVFVGSFPIFKIITALAVVGVVITAAYFLRMIQRMFLGEYNTKWEGLTEISGR
                     EIFTVIPLAILMIMVGVYPAVLANMINAAMENIVHLVLG"
     gene            8019..9482
                     /locus_tag="VGB16_04165"
     CDS             8019..9482
                     /locus_tag="VGB16_04165"
                     /EC_number="7.1.1.-"
                     /inference="COORDINATES: protein motif:HMM:TIGR01770.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0016020 - membrane [Evidence IEA];
                     GO_function: GO:0008137 - NADH dehydrogenase (ubiquinone)
                     activity [Evidence IEA];
                     GO_function: GO:0016655 - oxidoreductase activity, acting
                     on NAD(P)H, quinone or similar compound as acceptor
                     [Evidence IEA];
                     GO_function: GO:0048038 - quinone binding [Evidence IEA];
                     GO_function: GO:0050136 - NADH dehydrogenase (quinone)
                     activity [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="NADH-quinone oxidoreductase subunit N"
                     /protein_id="HEX9916911.1"
                     /translation="MKVDLLMLSPQIFLFLWAILIFVFDFFWNKEKKHNLGYVSLLGI
                     GITLVLVFLTTPGSLFGDMFLSDNFSRFFNLIFLGSAFLAVLSSIDYLKNVKYNRGEY
                     FSLILLSTVGMMLLSSANEFLFLYVSLELTTIPLFILTAYLKDNYKSSEAGLKYLILG
                     AISSAMLLYGISLIYGLTGTTSLNLIPSTLTALFLRTDVFDPALALALIFLTAGLGFK
                     LALVPFHMWAPDVYEGAPTPIAAYLSVASKAAGLVAFIRIFFHQYFLLLQIRTVDWIT
                     LVAVLAAFSMILGNIVALLQTNIKRMLAYSSIAQVGYILIGIVAASARGIASVGFYVL
                     AYLFANLGAFIVAIAFSNSTSSDNIPDYAGLSKRSPSLAIFMSIFMLSLVGIPPLAGF
                     VGKYYLFSAAIERGYIWLVVIAILTSVISLYYYAGVIREMYFRASPEQSKLKISPSLK
                     IAIIISILGVLICGIYPQPFIQLAQQAAAIFQYPTIR"
     gene            9529..10725
                     /locus_tag="VGB16_04170"
     CDS             9529..10725
                     /locus_tag="VGB16_04170"
                     /inference="COORDINATES: protein motif:HMM:NF013832.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEX9916912.1"
                     /translation="MLSKFKLILISFSAITGKAISAIWTFPSLIFSAFVIAWAAETAQ
                     FLVSQGLALALLAWLQTLPEFAVEAAIAWEAGKDIAKIHLITANFTGSLRLLVGLGWP
                     MIYFSRAFLGKKHLSAKRFSPIQLEDEHSVEVMGLLLPLIYFVIIYLKGTLGLFDSVI
                     LTSIYLAYLYILKKIPPQNEEKLQDACYVARKVMSFRPAGRYAAVFGLFLFGGVILYF
                     TAEPFLHSMLGLAVVFGISDFVFVQWVAPFLSEFPEKLSAFNWARTGKKAPMALMNMV
                     SSNINQWTVLVAMIPIVYSLSAGGISTVHFDQHQRLEILLTIAQSTLGFLLLANMKFH
                     WYEALTLFVLWFSQFIYPSIREEITVIYFVWIALELILGLVQKRQFVAFEKFGTLIKK
                     YVLRKK"
     gene            10960..12261
                     /locus_tag="VGB16_04175"
     CDS             10960..12261
                     /locus_tag="VGB16_04175"
                     /inference="COORDINATES: protein motif:HMM:NF013741.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0016020 - membrane [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="hemolysin family protein"
                     /protein_id="HEX9916913.1"
                     /translation="MLSSWIEIILILVLIVANGFFVSAEIALISLRRSKVRQLIDQGR
                     KNAKLIKKLQDEPYRFLATIQIGITLIGTLAAVVGGAELVDLIRPLIQKIPLSITQKG
                     SEPVAIGVVVILIAYFSLVLGELVPKYFALSYSEKIATKVAKPIYLLSRLSFLIVKLL
                     SASSRLVTHLLIGKTLIERSFIGEEEIKYIISEGKEKGVFEPTEEELIHRVFEFTDTY
                     VRTAMTPRNEIAALDIDTPQEKVIQTILEKGYSRIPVYKNNLDNVVGIIYVKDVINIL
                     QNNQLIILNDIIREPYFVPDSRKITQLLKEFQAKKIHIAIVQDKFGGTAGLITLEDIL
                     EEIVGEIRDEYDQEAELIQMLSDGSAIISVRTSLEDFNKKFETKLPQDKFGSVGDYLT
                     HHLGRIPALDEEIEIGNLKFIIFEKSTHRLSKIRVTKLPKK"
     gene            12271..12879
                     /locus_tag="VGB16_04180"
     CDS             12271..12879
                     /locus_tag="VGB16_04180"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_020168668.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_process: GO:0055085 - transmembrane transport [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="DedA family protein"
                     /protein_id="HEX9916914.1"
                     /translation="MIETILAPIAQWIIHLISYAGYWGIILAMTIESACIPLPSEIIM
                     PFSGYLVTTGRFNLWMVSLAGALGCLVGSTVAYWVGVWGGRPFLSRYGKYILISHKDL
                     DTADRWFSKYGDWAIFFSRLLPIIRTFISLPAGIARMNFTRFAIYTFVGSLPWCFALA
                     YIGKILGENWDKIKIYFQRADLLIAILFLVAIALFIYRHIKK"
     gene            12876..>13614
                     /locus_tag="VGB16_04185"
     CDS             12876..>13614
                     /locus_tag="VGB16_04185"
                     /EC_number="1.-.-.-"
                     /inference="COORDINATES: protein motif:HMM:NF013434.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016491 - oxidoreductase activity
                     [Evidence IEA];
                     GO_function: GO:0071949 - FAD binding [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="FAD-dependent oxidoreductase"
                     /protein_id="HEX9916915.1"
                     /translation="MTVPYWLDQSKQNPPIEADIAIIGGGIMGASLLYWLSKHKDIKV
                     ILLEKSRLASAASGRNAGFLIAGLAEHYNRSREHLGKAKSKQIWQLSLDNHRLLREEI
                     IERNKLECEYQKCGSYLLACSEVESKEIKETVELLNQDGFEVELIDERKINQLLKSQR
                     FHNACFSPKDGALNPVRLIEGIRAIIKSDTNIFEEHEVIDLEMVENSKVKIRTNHRTF
                     LAQMVVLATNAYSRSLFGVFKDLVIPTR"
BASE COUNT         3526 a         2750 c         3124 g         4214 t
ORIGIN      
        1 gatatggttc aacagttcga cgagggcttc gactgagtcc ttcgacggtg agccgctcgg
       61 gacggtcgaa ccgctcagcc gaacgtcttc actgtcctga gcaaagtcga aggacaagtt
      121 caccatctct tcgagcctga gccttccttc gacactgagg ctcaggacga agtgcagagc
      181 cgaaggcctg agcaaggtcg aaggaaattc ccacacctac gatgcgttcg gtttgtagca
      241 cagggaattt atccctgtgc ggggagccag attaaacaat gagatatgac ataatcttat
      301 gaccatagag gaaatcactc aaatactgaa taacaagttt ccggaaaagc taaaaatcat
      361 tgaggtaact agtcaccaac cggctttttt cattgagaaa gaaattctct tggagatatg
      421 taaattcgct aagcaatctg accaattgaa aatagatttc gtcaatgcca tctttgcggt
      481 agacaggact gacagcttgg aaatagtcta tgtcttattc tccttggaga agagacacag
      541 attatgccta aaagcgaaac tcccacgaaa ggatgcggaa atagattcag ttacccaagt
      601 ttgggaagcg gctaattggt ttgaaaggga gacatctgag cttttcggaa taaagtttaa
      661 gggacatcct gatcccaggc cactgctttt agcagaagat tggagtgaag gttatcctat
      721 gttaagggat tgggaaggga aagatttcat aaaacttcca cctaagtaga ttatgccgga
      781 agagatacac gcagaagagt ttatggtcaa tatgggtccc cagcacccca gcacacacgg
      841 ggttctgagg ttaatgttga ctatggatgg cgaggtggtg ttagatgcta tcccgcatgt
      901 cggctatctc catcgtgctc tggaaaagat agctgaaaac cgcacctttc tgcaatacat
      961 cccctatacc gacagattgg attatgtttc cgctatgttc gggaattacg cttttgttta
     1021 tccaattgag aaattagcca atgtagtcgt cccggaaagg gcagagtata ttcgggttat
     1081 catgatggag ttaaaccgca tcgcctcaca cctcatctgg ctgggaactt tctctttgga
     1141 tttgggagcg attactccgt ttctgtattg ttttcgcgag agggaacaaa tcttggattt
     1201 atttgaaatg gtctgtggtc aaaggatgac tttcaattat atgcgagtcg gtggcgtatc
     1261 caaagacctg ccggacgaat tcgttcctat ggctagaaaa tttgtagatt atttcaagcc
     1321 cagagtggat gagtatgagg agattttgac tcacaacccg atctttttgg gaagaaccaa
     1381 aggtattggg ttattggaca aggaaactgc ggtggagcat tcggtttctg gaccttcttt
     1441 aaggggatgt ggaataaact gggatttgag aaaaaatgag ccttactcaa tttatgaccg
     1501 gtttactttc gagatacctc tgggaaaagc gtctgatgtt tgggatagat atgaggttag
     1561 aatccaggag atgagacaag ctaatcttat agttgaacaa gccctaaagc agcttcctca
     1621 aggcgaattc aagacaaaaa ttccggcagt tttcaaaccc ccggctggag aggtttacac
     1681 tcgaattgag gctccccgag gggagatggg attttatata ataactgatg gaacgaccaa
     1741 gccgtatcgc gtcaaaatca gaaccggctc gttctgcaac ctgacggtct tgcccaagct
     1801 ttgcaagggc tggaaaatag cagacctggt ggctattttt ggaagtctgg atgtggtctt
     1861 gcctgaggtg gatagatgaa aagttaggca attacctaac ttttcatcct gagcccgcct
     1921 ttggcgggag aaggatctca agaataaata tggaaattaa gaggatcgga tagacgatgt
     1981 tagaattgga caaactgatt gaagccatct atttttggtt agagaagatt tttgttgatt
     2041 taggaattcc taagttcttc ttagacctgc tcagcatgct cctggtggcg cttgtgatta
     2101 ttatctttgc cgcactttca gtgatgtttc tggtctggtg ggaaagaaag atttcaggac
     2161 actttcagtc ccgctttgga cctatgcggg tgggctggca tggctggcta cagaccatag
     2221 ttgatgccat aaaactgatt atgaaagaag acatcgtccc cacaagtgcc gataaggttg
     2281 tcttttatat cgctccgata atacttttca tctccgctct tctggcttat atcgtcatcc
     2341 cttttggcaa aggtttagtg gttagagact taaatatcgg gatactgtac atcttagcca
     2401 tcaccacctt caccgtaatc ggaattttaa gcgctggctg gagctcgaac aacaaatatt
     2461 ctctcttggg tggtttccgc tcagccgctc agataataag ttatgaggtg cctttaacct
     2521 tatcagtttt aggggtagtg cttttggctg gctctatgag tctggtaact atcgtgaaaa
     2581 gccaaaacgg gattgtgcac tggtttatct ggaaacaacc attgggattt ttaatctatc
     2641 ttactgcggc aatcgcggag gtgaacaggg ttccctttga catacctgag gcggagcagg
     2701 aaatagtggc tggctacaac gtggagtaca gcggaatgaa gtttgcgatg ttcttctttg
     2761 cggaatatat gaacaccttt ctaatatgtg ctattgccac caccttattt ttgggaggat
     2821 ggaacggtcc gattctccca tcgtacgttt ggttttttat taagacattt ttcttgattt
     2881 tttgtctgat gtggcttaga tggacttatc cgcgcgtaag ggttgaccag ttaatggatt
     2941 tcgcctggaa gttcttgctt cctttggcgt ttttaaatct tatcgtcacc gcacttttag
     3001 cttaaggcat tatgtctata ctgaagaaaa taattgatgg tatcaaaaac ttaatcttgg
     3061 gattaggcgt aactgtaaaa tacttgctca agccggctgt aactttacag tatcccactg
     3121 agagatggac tatgcctgaa aggtcgcgag gcatggtgtc cttattgact gacaaggagg
     3181 caaagagatt aagatgcact gggtgcagtc tgtgtgtgag aatatgtcct aacaatgcta
     3241 ttcaaatggt tcctaagaag gatgagcaag ggaaaagaat gccggatacg tttgtggttg
     3301 acgttggcat gtgtatttat tgtggcttgt gccaggaggt ctgtccattt aacgccataa
     3361 ggcttgttcc caaatacgaa ttctcagttt atgataaaaa aagtctatcc tttgataagg
     3421 agaagttagc ggaaatcgga aaggattacg agtaggtagc ttatggtaag cggaactcaa
     3481 attgcggcat tttacatttt ggcgggaata atcatctgtt caagcgtaat ggtggtaact
     3541 atgaagaaca tattccattc agccctattt ctggttttat gcttcttcag tgtagctgga
     3601 atatatatcc ttttagaagc ggagttcgtt gccgcagttc aggttttaat ctatgtcggg
     3661 gcaatcaccg tgcttatgat ttttgccata atgcttacct ctcaaattta tagccccagc
     3721 ataaaacaaa gcaatgaaca ggtcattcct gccttattga ttgtagttgc acttttaatt
     3781 gtaaccttag taagtttagc caaaaccacc tggagaatca acacagcctt acctacggaa
     3841 cagtcaactg tgactattgg aaaatcgctt ttgacagttt atgttttgcc ctttgaggta
     3901 gtttcagtgg tgcttttggt ggctttggta ggagcaatcg ttatcgctaa aaaggagtaa
     3961 ggtttgctgg gtttaattca ctacttaact ttagctgcaa tccttttctg tgttggcata
     4021 tttggagtga tgacccgaag gaatgccatt ggggtgttga tgtccttgga gcttatgttc
     4081 aatgcggtga atataaactt tatcgctttc tccaagttta taacgccaac catcttaacc
     4141 ggacagatat tttccatctt tgtgataacc attgctgcgg ctgaggcggc tgtgggattg
     4201 gctattgtca ttctgattta ccgcaactat aagggcataa atgtggataa gataaacttt
     4261 atgaagtggt agggacaatt cattaataca gttaaagacg tcattctgag gcgctagccg
     4321 aagaatctcg agtttataat atgttggaat tgttagattc ttcacttcgc tcagaatgac
     4381 ataaaaggac attcttatgg tttctgacag gctcaataga tatttatagg aataagaaat
     4441 gttagcttat agttggatca taccattttt gcctttgctt gcctttgcca gtatagtgct
     4501 ggtaaccaat cctgacaaga aactcagctc ctttttatcc attggagcga tagtcacctc
     4561 tttcttgttg tcgattgtag tgttaataga ggtgatagcc agtccagttt ctaaagagtt
     4621 ttccatcagt tggcttaata tagctggctt caaggttgat ttcggaatgt tgattgaccc
     4681 cttgacttca gtgatgctaa tggtggtaac tatcgtctct atgctggtgc agatatattc
     4741 agttggatat atggcaggtg accctcgctt ttccagatat tttgccttct tgtctctgtt
     4801 cactttttca atgttagggt tagttttatc caacaatttc ttctccatat tcatcttctg
     4861 ggaattggtg gggttgacct cgtatctttt gattggattc tggtttgaga agaaaaccgc
     4921 ctcggatgcg ggcaaaaaag cattcatcac cacccgcact ggagatttgg ggtttatcat
     4981 tggaatcctg attctgacca ctgcggctgg gacctttaat ttccagtcta tcttcggaaa
     5041 aatcgaggct gggaccatcc ctgctggttt ggtgacatta tcagctattt ttatctttgc
     5101 cggagcggtc ggaaaatcag cccaattccc tttacatgtc tggctgccgg atgccatgga
     5161 aggtcccaca ccggtcagtg ctcttatcca tgcggcaact atggtagcag ctggagttta
     5221 tttagtggct cgctgtatgg gcgtctatgc tgcttcagaa tctgctggtt tggtagtagc
     5281 aatcatagga gtcttgactg cctttatggc ggcttcaatc ggattagtgc agaacgacat
     5341 aaaaagagtt ttggcttatt ctaccatcag ccaattggga tatatgataa tggctctggg
     5401 tttgggcggc ctcaccgctg gaaccttcca tttgatgacc catgcttttt ttaaggccct
     5461 tttatttttg ggtgcgggaa gcgtaattca cgctatacac accaataaca tctgggaaat
     5521 gggcgggctt tacaagaaga tgaaaatcac tgccataacc tttatcatcg cctctttatc
     5581 tataagcggc atatttcctc tttccggttt ttggagcaaa gacgagatac ttgctgtggc
     5641 aagcgaaaaa catccgctgt ttttgataat tgcggctctg gtagcaggaa tgaccgcatt
     5701 ttatatgttc cgactttgtt ttgtgacctt ctttgggaaa ccaaaagacc aggagaaatt
     5761 ccatcatgct cacgagtctc ccaaatctat gaccatacct ttatgcattt tagcattctt
     5821 atctatcttc gctggatggg tgggcattcc ctggcttcct ggcggatatt ctttctttgt
     5881 tttctatcac catccacatc atgctgaacc gaactacatt ttgatggtag tctcaaccct
     5941 cgtcgctttg tccggcatag gattggcata tttagtttat tacaaaggga ctttctcacc
     6001 agatagactc agggagagat ttaaacccat acataaagtt ttgtataaca aatattatgt
     6061 ggacgaattt tataatatga caattgtgaa tccgattttg aaactatcgg atatactttt
     6121 taaattcgat ttaggcgtaa tagactggct ggtaaacgga gttggcaatt tcaccgtttt
     6181 tctctcaaat gtgaaggagt ggttcgatag ccatatagtc gatggtgcgg tgaatggagt
     6241 gggttatgtt atcaaaggga ttgggtcagc tacccgaaaa attcagaccg gacaacttca
     6301 gaattacgcc tttattatat tctttgcaat tgtgataata cttttgataa agttgggtta
     6361 actaaagaaa aaaattggta tccctgcctt gccctcaagg cgggatatgc ttgaccgacc
     6421 catcttgcct gcccggccag ggagggcaag atgggtttac cggtttgtaa ttgtgataat
     6481 acttttgatt aagttaggtt aatcttgaaa attaaatcag gaggaaatag agactgatga
     6541 actttccaat tttgacttat atgactttta tccctctttt ggggatgata gtgatactat
     6601 ttattaacaa ggaaaaaatc cagcttgtgc gctggaccgc agccctgttc tcttttgtgc
     6661 ctttgcttct ctcgtttatt cttttatccc gattcaatag ctccacccct gagatgcagt
     6721 ttatggaaaa actggagtgg attccctcct taggagtaag ctacatcatg ggagcagatg
     6781 gaatctcggt gccgatgctc ttcctaactg cacttttatc attcgtttca gtaatagcgt
     6841 ccttcaatat cgacaagcga gtcaaagagt attttgcttt cttcttgctt ctggaaaccg
     6901 ggatgatggg agtcttctca gctttagatt tcttcctgtt ttatatcttc tgggaagtga
     6961 tgctggttcc aatgtatttc ttaatcggca tctggggagg tcccagaagg gagtatgcag
     7021 ccatcaagtt cttcttatac acccttttcg gaagcttatt catgctggtt ggaattctgg
     7081 ctctatattt tacggtcgag ccgcataccc tgaatatgat ggagctgatt aagaaagctc
     7141 cggattacgc cagaggattc cagcttctat cttttgtagc tttctatata gcatttgcca
     7201 ttaaggtgcc ggtgtttccg tttcacactt ggttgccgga tgcccatgtt gaagcaccca
     7261 ccgcggtttc agttatcctg gcaggaatct tactgaaaat gggaatctac ggtcttttga
     7321 gaataagcct gcctatgttc ccctacggag caatctattt tgctctgcct ttagccattt
     7381 tgggactggt caatatcgtc tatggtgctc tggttagtat ggctcaaaag gacttaaaga
     7441 aattagtggc atattcttcg gttagccata tgggatattg catcttaggc atatctgccc
     7501 taactgttac tggtctatcc ggatgtgtgt ttcaaatgtt ttcgcatggc ttaataaccg
     7561 gagcgctctt ccttctggtg ggagtgttat atgatagagc gcatacccgt gacatcgatg
     7621 ccttcggcgg cttgtgggtc aaacttccgg tctattcagc ccttatgatc ttattctgta
     7681 tgggctcctt agggcttccg ggcttatccg gcttcgtgag tgaatttatg gtattcgttg
     7741 ggtcttttcc aattttcaag ataataaccg ccctggcggt tgttggcgtg gttataactg
     7801 cggcatattt cttgaggatg attcagagga tgtttttggg agagtataat acaaaatggg
     7861 aagggctaac tgaaatcagt ggtcgggaga tcttcacggt tatcccactt gctattttga
     7921 tgataatggt tggggtttat ccagctgttt tggctaatat gataaatgct gctatggaga
     7981 acatagttca tctggtctta ggataaatgg aggattcttt gaaagttgat ttgcttatgt
     8041 tatctcctca gatattcttg ttcctgtggg caattctgat tttcgttttc gacttcttct
     8101 ggaacaagga gaagaaacat aatctgggat acgtctccct tttggggata ggcataactc
     8161 ttgttctggt tttccttacc acaccaggaa gtctgttcgg agatatgttc ctgtctgata
     8221 attttagccg tttcttcaat ctcatattct tgggctccgc ttttttggct gttctatctt
     8281 ctattgatta tctcaagaat gtcaaataca atcgaggaga atatttctcg ctaatccttc
     8341 tctccacagt ggggatgatg cttttgtcct cagccaacga atttctgttt ttgtatgtct
     8401 ctttggagct aacaactatc cctcttttta tcctgacggc ttatctcaaa gataactaca
     8461 aatcgtcaga ggctggattg aaatacctga tcttaggcgc catttcctcc gctatgctcc
     8521 tttatggaat aagcttgatt tatggattga ctggcactac ctcgctcaat cttattcctt
     8581 ccaccctgac agccctcttt ctcaggactg atgttttcga cccggcttta gctctggctc
     8641 taatattctt gactgccggt ctcggcttca agttagctct ggtgcctttt catatgtggg
     8701 cgccggatgt ttatgagggt gcaccaactc caattgctgc ttatctatct gtcgcctcca
     8761 aagccgctgg cttggttgct ttcattcgaa tattcttcca tcaatatttc ctgctcttac
     8821 agataaggac ggtggactgg attactctgg ttgcagttct ggcagccttc agcatgattc
     8881 tgggaaatat cgttgcgctt ctacagacca atataaaaag gatgttagct tattccagta
     8941 tcgctcaggt gggatatatc ttaattggaa tagtggctgc ctcagccaga gggatagcct
     9001 cggtaggatt ttatgtcttg gcttatctgt ttgccaatct cggagctttc attgtggcta
     9061 tcgccttcag caattccact tccagcgaca atatccccga ctacgccggg ctatctaagc
     9121 gctctccgtc attagctatc ttcatgtcga ttttcatgct ctctttggtc gggattccac
     9181 ctttagcagg ctttgttgga aaatattatc ttttctctgc ggcaattgaa agagggtata
     9241 tctggttagt ggtcatcgcc atcttgacca gcgtgatatc cctgtattat tatgccgggg
     9301 tgataagaga aatgtatttt cgtgcctctc ctgagcaaag caaactaaag ataagtccga
     9361 gtctgaaaat cgccataatc atctccatcc tgggtgtctt aatctgcgga atctatcctc
     9421 aacctttcat ccaattagct caacaggcag cagccatatt ccaatatccc acaattaggt
     9481 gatttgaggt tgacatttca cccaaaaaaa ctaacttagg gtagaaatat gttatccaaa
     9541 ttcaaactga ttctcatctc tttcagtgcc attaccggca aggcgatttc tgctatctgg
     9601 acctttccct ctctgatttt ctctgccttc gttattgctt gggcggccga gactgctcaa
     9661 tttctagttt cacagggatt agctttagct cttctggctt ggcttcagac cctgcccgaa
     9721 ttcgcagttg aggcggctat tgcctgggaa gcgggcaaag acattgccaa aatacattta
     9781 attactgcta atttcactgg ctccttaagg cttctggttg gtttgggttg gcccatgata
     9841 tatttttccc gcgctttcct tggaaaaaaa catctctcag ctaagagatt ttctcccata
     9901 caattggagg atgagcattc agtcgaagtg atgggattgc tcttgccttt aatctatttc
     9961 gtgataatct atctcaaggg gactttaggt cttttcgatt cagtgatatt aacctctatc
    10021 tatcttgcct atctatatat cttaaaaaag attcctcctc agaatgaaga gaaactccag
    10081 gacgcttgct acgtcgcaag gaaagtgatg tcattcagac cagcaggtag atatgctgca
    10141 gttttcggtc tatttctgtt tggaggggta atcctctact tcacagctga accattcttg
    10201 catagcatgc ttggtttagc cgtcgtcttt ggaatctcgg attttgtttt tgtgcagtgg
    10261 gtggcaccgt tcttatcgga atttcctgaa aaattaagcg cattcaattg ggctcgaacc
    10321 ggcaaaaagg cacctatggc tttgatgaat atggtctcct ccaatatcaa ccaatggact
    10381 gtgctggtgg cgatgatacc tattgtctat agtttaagcg ccggtggaat ctcaaccgtc
    10441 cattttgacc agcatcagcg tctggaaata cttttgacta tcgctcaatc gaccttgggg
    10501 tttttgcttt tggctaatat gaaattccat tggtatgaag ccctgaccct tttcgtgtta
    10561 tggttctcgc aattcattta tccgagcata agagaagaga taaccgttat atatttcgtg
    10621 tggatagccc ttgaactgat tttaggactg gtccagaaaa ggcaatttgt tgcttttgaa
    10681 aagtttggaa cgcttatcaa gaaatatgtc ttgcggaaaa aatgaccttt cccgcccaaa
    10741 gagcggggca ttcaaaacac ctcctccccc ttgaggggga ggactaagtt gaaaatctcg
    10801 ccaaaggcgg agagagggtg agaatcatct tcgctatccc cctcacccct tcgcttcgct
    10861 ttagggcagg ctctaaccct ctccccttgg gggagaggga acctatagaa atgtcattta
    10921 tccccctacg ggggtattct gacacatgtt tcataaaata tgcttagctc ttggatagag
    10981 ataattctga ttttggttct gattgtggct aacggcttct ttgtctcggc ggaaatagcc
    11041 ctaatctctc tacgtcgcag caaggtcaga cagcttatcg atcagggacg taagaatgct
    11101 aagctaataa aaaaactaca agatgaacct taccgctttc ttgccactat ccagatcgga
    11161 ataaccctaa tagggacttt agcagcggtg gttggtggag ccgaactcgt ggatttgatt
    11221 agacccttaa ttcaaaaaat ccccctatca attacccaga aaggtagcga acctgtggcg
    11281 attggagtcg tggttatctt aatcgcctat ttttctttgg ttctaggtga attggtgccc
    11341 aaatatttcg ccctaagtta ttccgaaaaa atagccacca aggtggccaa accaatttat
    11401 ctgctttccc gtctatcttt tttgatagtg aaactgctta gtgcctccag tcggctggtc
    11461 acccacctgc tgattggaaa gactctaatt gagagatcct ttatcggaga ggaagagata
    11521 aaatacatca tcagcgaagg gaaagaaaaa ggtgtcttcg agccgaccga agaggagctg
    11581 attcaccgag tttttgaatt caccgataca tacgtgcgca ccgctatgac ccctcgcaat
    11641 gagattgcgg cattagatat cgatacgccc caagagaagg tcatccaaac aatattggaa
    11701 aagggatatt cccggattcc tgtctataag aacaaccttg ataatgtagt gggtataatc
    11761 tatgttaaag atgtaatcaa tatcctgcag aataaccagc tgataatctt gaacgatatt
    11821 attcgagaac cgtattttgt tcctgattcc agaaaaatca ctcagcttct aaaggaattc
    11881 caagccaaga aaattcacat agctatcgtt caggacaaat tcggaggcac agcaggactg
    11941 ataactctgg aggatatatt agaggagata gttggagaaa taagggatga gtatgatcaa
    12001 gaagctgagc tgattcagat gttgtctgat ggctcagcca tcatttccgt cagaacaagt
    12061 ctggaggatt ttaataagaa atttgaaacg aaactgccgc aggataagtt cggttccgtc
    12121 ggggactatt tgactcatca cttgggaaga attccagctc tggatgagga gatagagata
    12181 gggaatctaa aatttatcat cttcgaaaag agcacccacc gtctaagcaa gataagagtc
    12241 actaaactgc caaaaaaata gaggatattt ttgatagaaa ctatactggc tccaattgcc
    12301 cagtggataa tacacttaat ctcttatgct gggtattggg gcattattct ggctatgacc
    12361 atagagagtg cttgcattcc cctgcccagc gagataatta tgcccttttc cgggtatctg
    12421 gtcacaactg gaagatttaa tttatggatg gtctccttgg caggggcttt gggatgtctg
    12481 gttggttcaa ctgtggctta ttgggtgggc gtatggggag gcagaccatt tctgtcaaga
    12541 tacggcaagt atattttgat ttcccataag gatttggaca cagctgatag atggttttct
    12601 aaatatggag attgggccat atttttctcc cgtcttttgc cgataatacg aaccttcatc
    12661 tctcttccgg ctggtattgc caggatgaac ttcacccggt ttgcgattta cactttcgta
    12721 ggttcgctcc cctggtgttt tgccttagct tatattggaa aaatcctggg tgaaaattgg
    12781 gataagataa aaatatattt tcaaagagcg gacctcctaa tagctatatt gtttttggta
    12841 gccatcgcac tcttcattta caggcatata aaaaaatgac cgttccttat tggcttgacc
    12901 aaagcaagca aaatccacca attgaggcgg atatcgccat aattggtggt ggtataatgg
    12961 gagcctcttt gctctactgg ctgagcaagc ataaagatat taaggttatt ttgctggaga
    13021 agagtcgtct ggcttcagcc gcttccggca ggaatgctgg atttctgatt gctggcttgg
    13081 cggagcatta caatcgctcc agagagcatc tgggaaaagc taagtcaaag caaatctggc
    13141 aattgagctt ggataatcac cggcttctaa gggaagagat aatcgagaga aacaaactgg
    13201 aatgtgaata ccaaaagtgc ggctcttatc ttctggcttg ttcggaggta gagtcaaagg
    13261 aaataaaaga aacagttgag ttattgaatc aagatggatt tgaagttgaa ctgatagatg
    13321 agagaaagat aaatcaactt ctgaaaagcc agagattcca caacgcctgc tttagcccca
    13381 aggacggagc tttaaatcca gttagattga ttgaaggaat cagggctatc atcaaatccg
    13441 atactaacat ttttgaggag cacgaggtaa tagatttgga gatggtggag aattcaaagg
    13501 taaagataag aaccaaccac agaactttcc tggcgcaaat ggtggtcttg gctactaatg
    13561 cttattccag aagtttgttt ggagtattta aagacttggt tataccaact cgcg
//
LOCUS       DASWLN010000125         1526 bp    DNA     linear   ENV 20-FEB-2024
DEFINITION  MAG TPA_asm: Candidatus Zixiibacteriota bacterium isolate
            SMAG_U2949 k141_1073157, whole genome shotgun sequence.
ACCESSION   DASWLN010000125 DASWLN010000000
VERSION     DASWLN010000125.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37960594
            Sequence Read Archive: SRR10376091
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Candidatus Zixiibacteriota bacterium (soil metagenome)
  ORGANISM  Candidatus Zixiibacteriota bacterium
            Bacteria.
REFERENCE   1  (bases 1 to 1526)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 1526)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (26-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/12/2024 08:28:26
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 1,440
            CDSs (total)                      :: 1,416
            Genes (coding)                    :: 1,412
            CDSs (with protein)               :: 1,412
            Genes (RNA)                       :: 24
            rRNAs                             :: 1 (16S)
            partial rRNAs                     :: 1 (16S)
            tRNAs                             :: 21
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 4
            CDSs (without protein)            :: 4
            Pseudo Genes (ambiguous residues) :: 0 of 4
            Pseudo Genes (frameshifted)       :: 0 of 4
            Pseudo Genes (incomplete)         :: 3 of 4
            Pseudo Genes (internal stop)      :: 1 of 4
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..1526
                     /organism="Candidatus Zixiibacteriota bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_1073157"
                     /isolate="SMAG_U2949"
                     /isolation_source="soil"
                     /db_xref="taxon:2053527"
                     /environmental_sample
                     /country="USA: Oregon"
                     /lat_lon="42.99 N 123.63 W"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            complement(<1..93)
                     /locus_tag="VGB16_04190"
     CDS             complement(<1..93)
                     /locus_tag="VGB16_04190"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_010869808.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="roadblock/LC7 domain-containing protein"
                     /protein_id="HEX9916916.1"
                     /translation="MYKVLEELNKTTGITGSMIVGSDGIVIAADL"
     gene            complement(86..976)
                     /locus_tag="VGB16_04195"
     CDS             complement(86..976)
                     /locus_tag="VGB16_04195"
                     /inference="COORDINATES: protein motif:HMM:NF015232.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="roadblock/LC7 domain-containing protein"
                     /protein_id="HEX9916917.1"
                     /translation="MGIVILFSLIIVLVPLIVYGLSPEKFFFSILTGRVYALFLFEFM
                     WYSILLLIMLRLASAFIVFVEVLACMVYRLCLGVAFGLLIMVGGGVGLQMALDKGIWA
                     YTPVVLIHIISTPFVLKGFFNQMVLWDMRKKKILRQISEQDIPVAKPPESRDAAVEPL
                     SEAREMDWTSPLRYVKEYSGVEGVFLVDNEGLIVARATGKQIDDERLAPFMLGLEEAN
                     NQALRKLNEKKLNRVELYTSSRWINLTRIFNLILVTVANRYTDDLLNVRIIQAAEMIR
                     KYMRKRYTQEVLTEAEDKNV"
     gene            complement(1002..>1526)
                     /locus_tag="VGB16_04200"
     CDS             complement(1002..>1526)
                     /locus_tag="VGB16_04200"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEX9916918.1"
                     /translation="GYGTKAKTILGKLRVADPSSQVVKDLLVDLGEKVSEEKRKAMEK
                     IEDPIVKTFEGEVDLNEALDYLKTFPKVFGAAIVGLDGLILEERFRIDLEKDVLGAIS
                     ANLFDVVQSGLAEINLRGLEQVVVESGNWQLWTVRLEGKLLIICCSEEVNVGSLRMKV
                     RELLDHLTLKPKES"
BASE COUNT          418 a          406 c          257 g          445 t
ORIGIN      
        1 caagtcggcc gcgataacta tcccatctga gccaactatc atgctgcctg ttatccccgt
       61 agttttgttc aactcctcta agaccttata catttttatc ctccgcctct gttagtacct
      121 cttgagtata tcttttcctc atatattttc ttatcatctc cgccgcctga atgatacgca
      181 cattcagtaa gtcatcggtg taacgattgg ctacagttac caggatgaga ttgaatatcc
      241 gagtcaagtt tatccagcga ctcgaagtat agagttcaac tcggttgagc ttcttttcat
      301 ttaattttct caaggcctgg ttatttgctt cctccaatcc gagcatgaat ggagccagcc
      361 tttcatcatc tatctgtttg cctgttgccc tggcaactat caaaccctca ttatccacca
      421 agaatacacc ttctaccccg ctatactcct ttacgtaacg gagaggagat gtccagtcca
      481 tttccctggc ctcactcaaa ggttcaactg cggcatctct gctttctggt ggcttcgcca
      541 cagggatatc ttgttcggaa atttggcgaa gtatcttttt cttgcgcata tcccacagca
      601 ccatttgatt gaaaaaccct ttcaggacaa acggggtgga gattatatga atgagcacaa
      661 ctggagtata agcccatatt cctttgtcga gtgccatctg cagaccgacc cctcctccga
      721 ccataattag caaaccgaat gccactccca aacataagcg ataaaccata cacgctaaaa
      781 cctcaacaaa gactatgaag gcagaagcta aacgtaacat aatcaagagc aggatgctgt
      841 accacatgaa ctcaaacagg aaaagcgcat atactcttcc cgttaagatg gagaagaaaa
      901 acttctccgg agatagtcca taaactatca aagggactaa gacgatgatc aaagaaaaaa
      961 gaattactat tcccatttct tttctttggt ctggcctcaa tttaactttc ctttggtttc
     1021 aaggttaagt ggtctaacag ctctcttact ttcattctaa gggaaccaac attgacttcc
     1081 tctgaacaac atatgattag caacttgcct tccagacgaa ctgtccacaa ttgccaattt
     1141 cctgattcaa ccacgacctg ctctaatcct ctaagattta tttcagccaa tccggactgc
     1201 accacatcaa aaagattagc gcttattgcc cccaacacat ccttttctaa gtcgatacga
     1261 aatctctcct ccaagatcag accatccaat cccactatgg cagcgccaaa gactttagga
     1321 aaagttttga gataatctag ggcctcattc agatcaactt ccccttcaaa tgttttgact
     1381 atcgggtcct cgattttctc cattgctttg cgtttttcct cggatacctt ctcacccaaa
     1441 tctaccagta aatcttttac cacttgactg ctaggatctg ccacgcgtaa cttacctagg
     1501 atggtcttgg ctttggtgcc atatcc
//
LOCUS       DASWLN010000126         8373 bp    DNA     linear   ENV 20-FEB-2024
DEFINITION  MAG TPA_asm: Candidatus Zixiibacteriota bacterium isolate
            SMAG_U2949 k141_98057, whole genome shotgun sequence.
ACCESSION   DASWLN010000126 DASWLN010000000
VERSION     DASWLN010000126.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37960594
            Sequence Read Archive: SRR10376091
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Candidatus Zixiibacteriota bacterium (soil metagenome)
  ORGANISM  Candidatus Zixiibacteriota bacterium
            Bacteria.
REFERENCE   1  (bases 1 to 8373)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 8373)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (26-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/12/2024 08:28:26
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 1,440
            CDSs (total)                      :: 1,416
            Genes (coding)                    :: 1,412
            CDSs (with protein)               :: 1,412
            Genes (RNA)                       :: 24
            rRNAs                             :: 1 (16S)
            partial rRNAs                     :: 1 (16S)
            tRNAs                             :: 21
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 4
            CDSs (without protein)            :: 4
            Pseudo Genes (ambiguous residues) :: 0 of 4
            Pseudo Genes (frameshifted)       :: 0 of 4
            Pseudo Genes (incomplete)         :: 3 of 4
            Pseudo Genes (internal stop)      :: 1 of 4
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..8373
                     /organism="Candidatus Zixiibacteriota bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_98057"
                     /isolate="SMAG_U2949"
                     /isolation_source="soil"
                     /db_xref="taxon:2053527"
                     /environmental_sample
                     /country="USA: Oregon"
                     /lat_lon="42.99 N 123.63 W"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            169..2514
                     /locus_tag="VGB16_04205"
     CDS             169..2514
                     /locus_tag="VGB16_04205"
                     /inference="COORDINATES: protein motif:HMM:TIGR01069.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0030983 - mismatched DNA binding [Evidence
                     IEA];
                     GO_process: GO:0006259 - DNA metabolic process [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="endonuclease MutS2"
                     /protein_id="HEX9916919.1"
                     /translation="MDEHTFKVLEFDKVRDLVSTFCLSSLGKEMCQEMLPLVDEKLIQ
                     EKLKAASELKEVLLFEERFPLNSVKDIRPYLNKLQSSGTFLEPKELLDLAETLIISKS
                     VFSFLEPKKRKYQTLWDILSEMKPEDELIGVIEKVVDSSAQIKDTASKELRQIRQRKS
                     STRNKILDKLRSLLDSKRSKTSKQEDIITIREGRYVIPMETEEFSRSKGIVHDRSSSG
                     ATFFVEPFSVVELNNELRELDLEEKREIEKILLGISNLIRQDLEEIKQIVETLQLIDF
                     IHGKARFSIEFKANAPRLNTKGFVNLIKARHPLLEKKIREMIVPLDLELGEDFNTLII
                     TGPNTGGKTVALKTIGLLTLMAQAGMHIPVEDTSQIAIFDKIYADIGDEQSIEMSIST
                     FSSHISRVIKAVSDSDTNTLVLLDELGAGTDPKEGVALAEAVLINLSARNAKTVITTH
                     YGILKLLPQNHPQMRNASLEFDRETFKPTYNFRPGLPGSSYAIEVAQRLGMPKDVLDK
                     ASELVGTQERDLSLLLESLEKDLKRVNQDRRTLEEQKKISDELLNLYQDKLRKIELKE
                     KELERKSLEEARRLVDQTKIELERLVAQIRSTQADKELVKKTHQFIDKTEKEIKDKLK
                     ISKDKKTEEQNIEKGDVVWIDSLKVQGEVISKQGDQVRVRAANFTYTVGREEISKVET
                     AKEKPVQSGSISSSEAQGASLEVDLRGLYGDEAVEVVDKCLDRAYVSNLNTLSIIHGK
                     GTGALRKRINLFLRRHPRVDSFRLGNWNEGGSGVTVVKLKE"
     gene            2750..4180
                     /locus_tag="VGB16_04210"
     CDS             2750..4180
                     /locus_tag="VGB16_04210"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_013886454.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="aldehyde dehydrogenase family protein"
                     /protein_id="HEX9916920.1"
                     /translation="MVKNYRMLIGGKWVDSDKKIEVTNKYNHQVVATVALANKELYEE
                     TVKSAQKGLELMSSMPAYQRSKILQTTSELLETNKKELTELIAVEAGKAWKHSLMEVE
                     RGIGTFKFASEEAKNIHSQTVPMDAIPGGEKRIGFILRFPVGMIGAISPFNYPLNLVA
                     HKVAPAIAAGNSVILKPASLTPLTSLKLGEIMMEAGLPDGALNVIIGSGSTVGEWLIQ
                     DERLSMITFTGSPPVGRRIKEKSGLKKVTLELGSNSAVIIDQSADLNSAVHRCVFGSF
                     SYAGQVCISVQRMYVHKKVISEFTQRFLDMTRKLKIGDPLKEDTDVGPMITEEDAVRT
                     EEWVNEAVKNGAKILIGGKREKNFYYPTVLTNVKPEMKVMRLEAFAPIVSIIEFEDFS
                     DAVRMVDDSIYGLQAGIFTAEIEKAFEAINKIKVGGVIINDVPTYRADQMPYGGVKES
                     GIGREGLKYAIEEMTDMKMVVFNLGK"
     gene            4197..4745
                     /locus_tag="VGB16_04215"
     CDS             4197..4745
                     /locus_tag="VGB16_04215"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEX9916921.1"
                     /translation="MNLLYVLYGVLAAGLGYVIKGVIDYFVEKRKMDLRIKETFAKNA
                     VETLQETQEWLYKINSAVDGYLEKPSEESRKEARDNCLKLFKEYKGEWYFKKSIYMEK
                     NVRKALDLFQDFAILKIFDIQENPLTGTYWDSLHKMIMVLQEALDKVAKEYNLLHDLD
                     LGDLVKKIDDERKKIIEEVQGK"
     gene            5168..6673
                     /gene="hutH"
                     /locus_tag="VGB16_04220"
     CDS             5168..6673
                     /gene="hutH"
                     /locus_tag="VGB16_04220"
                     /EC_number="4.3.1.3"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_018198145.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004397 - histidine ammonia-lyase activity
                     [Evidence IEA];
                     GO_process: GO:0006548 - histidine catabolic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="histidine ammonia-lyase"
                     /protein_id="HEX9916922.1"
                     /translation="MKTIFIDGEKLTLEQVEEVALKGTQVKISPSAKKKIAKCRRYVE
                     QVLESGKRVYGINTGFGKLCQMMIDKEDIGKLQENLIKGHAIGFGPVFSEAEVRAMML
                     LRANVLAKGYSGARLELVQRLVDMLNKKVHPVIPEKGSVGASGDLAPLAFIGLVLIGQ
                     GWAFYKDRVMDGRLALQKAGIKPIKLLAKEGLALVNGTQVMTAIAILNLLKAERLANL
                     SDVSGAMSLDAILGSPVPFDLDLQNTRRHKGQREVAIHLNRLMKNSQIREFHKACPKI
                     QDPYSFRCMPQVHGAVRDTLKYVRQVLEIEANSATDNPLVFANEDKVISGGNFHGEPV
                     ALAMDFLGIAMSELGNIAERRVFAMLDPSISGLPSFLTRRPGLNSGFMIAQTAAAALV
                     SENKILSHPASVDSIPTSINQEDHVSMGTISARKAREIINNVEDILSVEFLVAGQGID
                     LRKPLKPSPILQKVHNAIRRMVPELGDDRVMMGDLVKTKELVRMNFGMKEL"
     gene            7103..7294
                     /locus_tag="VGB16_04225"
     CDS             7103..7294
                     /locus_tag="VGB16_04225"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEX9916923.1"
                     /translation="MNRSASKLEKIVSYCIMVILLGLGGLILAGVFSVQTKFRFIIFL
                     IIVSYIAVRLVVMNRGSKS"
     gene            7418..>8373
                     /locus_tag="VGB16_04230"
     CDS             7418..>8373
                     /locus_tag="VGB16_04230"
                     /inference="COORDINATES: protein motif:HMM:NF024824.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="tetratricopeptide repeat protein"
                     /protein_id="HEX9916924.1"
                     /translation="MKPITSRKNFISKTILLLTLLVIAFSLALAQGLLDSVKEAYNKG
                     DYNKAIELSKQYLQDKPNDLDAHFYLGNAYHRKGDLDQALAEYKLILERKSKDPDVLY
                     ELGYAYYHKKTYDQAQEMFDAGLKTGKNRAEFFNALGLTQVAKGLLNEADFSFRSAIS
                     IDNKIAAYHKNLADANFKKQVYSIAAIEYKKATELDSTMAECHFSLGQCYFKQMDFNL
                     AAEEFKKAVLLDSTYYEAYANLGNLYMLAQRLGEAVISYEEYLKHNKKNGSVYFNLGK
                     AYYLLRQLDKALENLSRAIELNPKAIDAHYYLGQTYQDKKMN"
BASE COUNT         2704 a         1442 c         2052 g         2175 t
ORIGIN      
        1 caaaagcagt ccagcgagta tccttttagg gaggaaaggt attcgcggac tagcgaaagg
       61 actcagagaa taatccaaca gatggaagaa tattttggac cataatgcca ttatctgaag
      121 tcaaagtctt atcttgacca ctctcttctt caaatttcgc cccttcatat ggatgagcat
      181 accttcaagg tattagaatt cgacaaagta agggatttag tgtccacttt ctgcctatct
      241 tcgttaggca aggaaatgtg tcaggaaatg ttgccactag tggatgagaa gctcatccag
      301 gagaagctga aagccgcgag tgagctcaag gaagttttgt tatttgaaga aagattcccc
      361 ctaaacagcg taaaggatat aaggccttat ctgaataagc tccagtccag tggaactttt
      421 ctggaaccga aagagcttct ggacttagcc gaaactttaa tcatctccaa gtcagttttt
      481 tcctttctgg aacccaagaa aagaaaatac cagacattgt gggatattct ctccgagatg
      541 aagccagaag atgagctgat tggtgtcata gaaaaggtag ttgattcttc agctcagatt
      601 aaggatacag catcaaaaga acttcgccag atcaggcaac gtaaaagctc cactcgaaat
      661 aaaattctag ataaattgcg ttccctgctc gattccaaac gcagtaaaac ttccaaacag
      721 gaagatatca taaccattcg cgaaggccgg tatgtgattc ccatggagac ggaggaattc
      781 agtcgcagta agggaatcgt gcatgaccgt tcgagcagtg gtgcaacttt ctttgtggag
      841 ccctttagcg tggttgagct aaataatgaa ttaagagagt tggatctgga agagaagagg
      901 gagatagaaa agatacttct ggggatttca aatctcatac gtcaggattt agaagagata
      961 aaacaaatag tcgagacact gcagttaata gattttattc atgggaaggc gcgtttttca
     1021 atcgaattca aagccaatgc tcccagactg aatacaaagg gttttgtaaa tctgattaag
     1081 gcgcgtcacc ctttgctgga gaagaagatc agagagatga tagtcccgct tgacttagaa
     1141 ttgggtgagg atttcaacac tttgattatc actggtccca atacgggtgg caagaccgtt
     1201 gctttgaaaa caattggact tcttacttta atggctcagg caggtatgca tataccggtt
     1261 gaggatacct ctcagatagc tatattcgat aaaatatacg cagatattgg agatgaacaa
     1321 tccatcgaga tgtccatttc aaccttctcc tcgcacataa gtcgggtaat caaagcggtc
     1381 agcgattcgg atactaacac tttagtcttg ctggatgagc ttggagctgg gaccgatcct
     1441 aaggaggggg ttgctttagc agaggcagtt ttgattaatc tctccgcaag aaatgccaag
     1501 acagtcataa ccactcacta cggtatactc aagctcctgc cccagaatca tccacagatg
     1561 cgcaacgcct cacttgaatt tgatagagag actttcaagc cgacttataa ttttcgcccg
     1621 ggtttgcccg gctcaagtta tgccattgag gttgcacaga gattgggcat gcccaaagat
     1681 gtcttggata aagccagtga gctagtggga acccaggaaa gagatttgag cttgctccta
     1741 gagagtctgg agaaggattt gaaaagagtc aaccaggata gaagaacttt agaggagcag
     1801 aaaaaaatat cagatgagct cttgaatctg tatcaggaca aacttagaaa aatagaatta
     1861 aaggagaaag aattagaaag aaaatcctta gaagaagcca gaaggttagt tgaccaaacc
     1921 aaaatcgagc tggaaaggtt ggtagcccag ataagaagca cgcaggcaga taaagaattg
     1981 gttaagaaaa cccaccagtt cattgataag acagaaaagg aaataaaaga caagctgaag
     2041 atttcaaaag acaaaaagac agaagagcaa aacatagaaa agggagatgt agtctggatt
     2101 gattctctca aagtccaagg agaggttata tccaagcaag gggatcaggt aagagtccgt
     2161 gctgcgaatt tcacctacac tgtgggtcga gaagaaataa gtaaggtgga aacggcaaag
     2221 gaaaaaccag ttcaatctgg aagtatcagt agttcggaag cacaaggggc atctttggaa
     2281 gttgatttaa gaggcctgta tggagatgag gcagtggagg tagttgacaa atgcctggat
     2341 agggcatatg tttcaaatct aaacacgctt tcaatcatac acggcaaagg aacaggagcc
     2401 ttgcgtaaaa gaattaatct ttttttaagg aggcaccccc gcgtggatag tttcaggctg
     2461 ggcaattgga acgagggtgg aagtggagtg accgtagtga agttgaagga gtgaggattt
     2521 agtttataaa taatgcaggc gacccatttg ggtcgcctgc aaaatgattg tgtgtcattc
     2581 ccgtgaagac cagcggcctt gcccacggct tcgccgtgag gcgagccgct aggcaggcgg
     2641 gaatccatat tttgattttt ctatcgcaaa ttgaatggat tcctgctgga gtttacccct
     2701 gcggaagcag gggcagaaat gacaagcaga ctaaagaggg agaagaaaaa tggttaagaa
     2761 ttacaggatg ctaattggtg gaaaatgggt ggacagtgat aaaaagatag aagtaaccaa
     2821 taaatataat catcaagtag tggcaactgt tgcgttagca aataaagagc tttatgaaga
     2881 aacagttaag tccgcacaaa agggcttaga gttaatgtca agtatgcctg cttaccaaag
     2941 gtcaaaaata ttacaaacta catctgagct tttagaaaca aataaaaagg aattgaccga
     3001 gcttattgcg gttgaagcgg gcaaagcatg gaaacattca ttgatggaag tggaaagagg
     3061 gattggtact ttcaagttcg cttcggaaga agccaaaaat attcatagcc aaactgtgcc
     3121 tatggatgcc atacccggag gagaaaagag aatcggtttt atcctgcgtt tcccggtagg
     3181 aatgatcgga gccatttcac cctttaacta tcctctgaat ttagttgccc ataaagttgc
     3241 accagccatt gctgcaggaa attcagtgat tttaaagcca gcttccttaa ctcctcttac
     3301 ctccctcaaa ttaggtgaga taatgatgga agcagggttg ccagatggtg cattgaacgt
     3361 aattatcggc tcaggcagta cggttggaga atggttaata caagatgaac gtttatccat
     3421 gataaccttt actggcagtc ctccagtggg aagaaggata aaggaaaaat cgggcttgaa
     3481 aaaagtaact ttggaattag ggtcgaattc agccgtcata attgaccaat cagctgactt
     3541 aaattcagca gtacaccgtt gcgtttttgg cagtttttcc tatgccgggc aggtctgtat
     3601 ttctgtgcag agaatgtatg ttcacaaaaa agttatttcc gaattcaccc aaagattttt
     3661 ggatatgacc aggaagttga aaataggaga cccgctaaaa gaggatacag atgtcggtcc
     3721 aatgataact gaagaagatg ctgtaagaac tgaagaatgg gtcaacgagg cagtgaaaaa
     3781 cggtgctaag atactcatcg gcggaaagag agaaaagaat ttctattatc ctacggtttt
     3841 aacaaatgtc aagccggaaa tgaaagttat gcgtttagaa gcattcgctc ccatagtttc
     3901 aataattgaa tttgaagatt tctcggatgc ggtgcgtatg gtggacgatt caatttatgg
     3961 gttgcaagca gggattttca ctgcggaaat tgaaaaagca tttgaagcca taaataaaat
     4021 caaagtcggc ggagtgataa taaatgatgt tcccacctat cgtgcagatc agatgcctta
     4081 tggtggggtc aaagagagtg gcattggaag ggaagggttg aaatacgcaa ttgaggaaat
     4141 gacggatatg aaaatggtgg tgttcaattt ggggaagtaa attaaaaggg aggtatatga
     4201 atttgttgta tgttctctat ggcgttttag ctgctggttt gggatatgta ataaaaggag
     4261 ttatagatta ttttgtggag aagaggaaaa tggatcttag gattaaagag acctttgcca
     4321 aaaatgctgt ggagacgctt caggagacgc aggaatggtt gtataaaata aattcagctg
     4381 tggacggata tctggaaaaa ccgagcgagg aatcgagaaa agaggcaagg gacaattgtc
     4441 ttaaactctt taaagagtat aagggcgaat ggtactttaa gaaatctatt tatatggaga
     4501 aaaatgttcg taaggcctta gatttatttc aggattttgc aatactaaaa atatttgata
     4561 ttcaggagaa tcctcttact gggacatact gggattctct tcataagatg ataatggtct
     4621 tacaagaagc gttagataag gttgcaaagg aatataatct tctacatgat ttggatcttg
     4681 gcgatttggt caagaagata gatgatgaac ggaaaaagat tattgaggaa gtgcaaggca
     4741 agtaaattgc ataactttct gtgtctgtca ggtcccccct gaaggtgacc gagaactcca
     4801 attgtgtcat tctgaacgga gcgaagcgga gtgaagaatc ttagttttat gctggttggg
     4861 aagcgagatt cttcgccctt acgggctcag aatgacaatt ctcgatcggc ctgccctgac
     4921 ctgacagggg attccgccaa gtcagcctgt gcccgtttct ccccgcttga cccaatggcc
     4981 aggggggact tggcggggca cggaatttga tttcaggtgt cattcctgcg caagcaggaa
     5041 tccattttgt attttagggt gagaaatctg gattcccgcc ttcgcgggaa tgacaatagc
     5101 gtggaaaagg aggagcctcg taatagagtc tggtaggaga agtgaatttt aaaatgtgga
     5161 gaagaaaatg aaaacaattt ttatcgacgg tgaaaaattg acgctggagc aggtggagga
     5221 agttgcacta aaagggacgc aggttaaaat atctccttct gccaagaaga aaatagctaa
     5281 atgcagaaga tatgtggaac aggttttgga aagcgggaaa agagtttatg gcatcaatac
     5341 cggttttggc aaattatgcc agatgatgat agataaagag gacatcggga agttacagga
     5401 gaatctaatc aaaggccatg ccataggatt tggacctgtg ttttccgaag cggaagttag
     5461 agcaatgatg cttctacggg caaatgtatt agccaaaggt tattccgggg caagattgga
     5521 attggtgcag agattggttg acatgctcaa taagaaagtt catcccgtaa ttccagaaaa
     5581 aggctcagtt ggagcatccg gggatttggc acctctggct tttatcggtt tggtattgat
     5641 tgggcaaggt tgggcatttt acaaagatag agttatggat ggtcgtttag ctttgcagaa
     5701 ggcaggtata aagccaatta aattgttagc caaggaagga ttggctttag tgaacggaac
     5761 ccaggttatg actgcaatcg ctattctgaa tctgctcaaa gctgaaagat tagccaactt
     5821 gtcagatgtt tcaggggcga tgagtttaga tgcgattttg ggaagcccag tcccttttga
     5881 tttggattta caaaacacaa ggcgacataa aggacaaagg gaagttgcaa ttcatctaaa
     5941 tagattgatg aaaaatagcc aaatcaggga gtttcataaa gcttgtccca aaattcaaga
     6001 cccctacagt ttcagatgta tgccccaagt tcacggagca gtgagagata ccctaaaata
     6061 tgttcgtcag gtgctggaaa tagaagccaa ttcagccaca gacaatccgc ttgtttttgc
     6121 caatgaagac aaagtcatat ccggtgggaa ttttcatggt gagccagtag ctttagccat
     6181 ggatttttta ggaatagcga tgtccgaatt gggaaatatt gccgagcgga gagtttttgc
     6241 tatgttggac ccatccataa gtggcctgcc cagctttctg accaggaggc cgggactgaa
     6301 ttccggcttt atgatagccc agaccgcagc tgcagcattg gtttccgaaa ataaaatctt
     6361 atcccaccct gccagcgttg attccatccc aacctcaatc aatcaggaag accatgtaag
     6421 catgggaaca atctccgcac gcaaggcgag agaaataata aacaacgttg aggatatatt
     6481 atccgtcgaa tttcttgtag ctggccaagg aatagatctt agaaaaccac tcaagcccag
     6541 cccaattctg caaaaagtac ataatgcaat tagaagaatg gttccagaat tgggtgatga
     6601 tagagttatg atgggggatt tagtgaagac aaaggagttg gtgcggatga attttgggat
     6661 gaaggaatta tagatattag aagttacgtg tcattgcgag gagtgccgat gaaatcggaa
     6721 cgacgaagca atcttgtagt aaaatcgaga ttgcttcgcc ctaggggctc gcaatgacaa
     6781 ttggaatatc agaaaaacag taatataact gcaaataagt cgacaaaata ctcgtagttt
     6841 ctattgagaa gtcgcaaatt tttgcgaacc gcgtaagttg ttaagtatga actgtttttg
     6901 ggcataaacc cacagatttt gtgctttagg tcgcaatctt ttgcggttat acgggtaagt
     6961 gaaagatagg gtagaatagc aagtcactaa gttattgtct aataaggtat tataactatc
     7021 aaaaaaaggg ctaaaaggtt gatttcggca cagaatttgt tcacctaatt atatggagtc
     7081 tcaatatcaa ggaagggaaa agttgaatcg ttccgcatcc aaattggaaa aaatagtcag
     7141 ctattgcatt atggtaatct tgttgggttt gggtgggttg attttggcgg gtgttttttc
     7201 ggttcaaacg aagtttcggt tcattatttt tctgataata gtttcctaca ttgcagtaag
     7261 gttagtagtg atgaatagag gaagcaaaag ttagtatttg ataaaaaaag cttgacagag
     7321 ttgttaaatt tggtatttta aaaattcata taatggaaac tttaaaattt gtaacaaatt
     7381 ttaaagtttc catttcagga aattcaaggt ggttcggatg aaacctatca caagtcgaaa
     7441 aaacttcatt tctaagacta ttcttcttct aacgttatta gtgatagcct tcagcttagc
     7501 ccttgcgcag ggtttgttag attcagtaaa agaagcatat aataagggcg actacaacaa
     7561 agccatcgaa ctttcaaaac aatacctgca ggacaaaccg aacgatttgg atgcacattt
     7621 ctatcttggg aatgcctacc atcgaaaagg ggatttggac caagccttag ctgaatacaa
     7681 attgattctg gagcgaaagt caaaagaccc agatgtgctg tatgaattgg gatatgcata
     7741 ttaccacaag aaaacgtatg accaagccca ggagatgttc gatgccggac ttaagaccgg
     7801 caagaacaga gctgaattct ttaatgcttt gggtctgact caagtggcaa aggggttatt
     7861 gaacgaggca gactttagtt tccgctcagc tatatccatt gacaacaaga tcgcggcata
     7921 tcataagaat ttagcagatg ccaacttcaa gaagcaggtc tatagcatag ctgcaataga
     7981 atacaagaaa gccactgagt tagattcaac catggcagaa tgccatttct ctttagggca
     8041 atgctatttt aagcagatgg atttcaatct ggcagcagag gaattcaaga aagcagttct
     8101 tttagattca acctattacg aagcttatgc taacctgggt aacctctata tgctcgccca
     8161 gaggttggga gaagcggtaa tcagctacga ggaatatcta aagcataata agaaaaacgg
     8221 ttcagtctat ttcaatctgg gcaaggctta ctatctgtta aggcaattgg acaaggcatt
     8281 agagaactta agcagggcga ttgaacttaa tccgaaagca attgatgctc attactactt
     8341 aggacagacc tatcaagaca agaaaatgaa taa
//
LOCUS       DASWLN010000127         2973 bp    DNA     linear   ENV 20-FEB-2024
DEFINITION  MAG TPA_asm: Candidatus Zixiibacteriota bacterium isolate
            SMAG_U2949 k141_1193280, whole genome shotgun sequence.
ACCESSION   DASWLN010000127 DASWLN010000000
VERSION     DASWLN010000127.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37960594
            Sequence Read Archive: SRR10376091
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Candidatus Zixiibacteriota bacterium (soil metagenome)
  ORGANISM  Candidatus Zixiibacteriota bacterium
            Bacteria.
REFERENCE   1  (bases 1 to 2973)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 2973)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (26-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/12/2024 08:28:26
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 1,440
            CDSs (total)                      :: 1,416
            Genes (coding)                    :: 1,412
            CDSs (with protein)               :: 1,412
            Genes (RNA)                       :: 24
            rRNAs                             :: 1 (16S)
            partial rRNAs                     :: 1 (16S)
            tRNAs                             :: 21
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 4
            CDSs (without protein)            :: 4
            Pseudo Genes (ambiguous residues) :: 0 of 4
            Pseudo Genes (frameshifted)       :: 0 of 4
            Pseudo Genes (incomplete)         :: 3 of 4
            Pseudo Genes (internal stop)      :: 1 of 4
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2973
                     /organism="Candidatus Zixiibacteriota bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_1193280"
                     /isolate="SMAG_U2949"
                     /isolation_source="soil"
                     /db_xref="taxon:2053527"
                     /environmental_sample
                     /country="USA: Oregon"
                     /lat_lon="42.99 N 123.63 W"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            complement(<1..833)
                     /gene="gpmI"
                     /locus_tag="VGB16_04235"
     CDS             complement(<1..833)
                     /gene="gpmI"
                     /locus_tag="VGB16_04235"
                     /EC_number="5.4.2.12"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_013277320.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0046537 -
                     2,3-bisphosphoglycerate-independent phosphoglycerate
                     mutase activity [Evidence IEA];
                     GO_process: GO:0006096 - glycolytic process [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="2,3-bisphosphoglycerate-independent
                     phosphoglycerate mutase"
                     /protein_id="HEX9916925.1"
                     /translation="MLLLLIMDGYGIREEKNGNAIALAKKPNLDNIFSNFPYTTLSAS
                     GLDVGLPKGLMGNSEVGHLNIGAGRIVYQEITRIDKAIEDGSFFKNQAFLEAIDIAKK
                     NNSKLHLLGLVSDGCVHSSPEHLYALLRLMKTNDLKKVYIQAFLDGRDTSPTNGVKYI
                     KELQDKLDEYKVGKIATISGRYYAMDRDKRWERIERAYRAMVFGEGVKNDDPILAIKE
                     SYAKKVTDEFVEPTIIDPSGIIQKGDVAIFSNFRADRARQLSWALTDSDFDEFPRPDN
                     LT"
     gene            complement(1165..2250)
                     /gene="purM"
                     /locus_tag="VGB16_04240"
     CDS             complement(1165..2250)
                     /gene="purM"
                     /locus_tag="VGB16_04240"
                     /EC_number="6.3.3.1"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_012302714.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004641 -
                     phosphoribosylformylglycinamidine cyclo-ligase activity
                     [Evidence IEA];
                     GO_process: GO:0006189 - 'de novo' IMP biosynthetic
                     process [Evidence IEA];
                     GO_process: GO:0009152 - purine ribonucleotide
                     biosynthetic process [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="phosphoribosylformylglycinamidine cyclo-ligase"
                     /protein_id="HEX9916926.1"
                     /translation="MKRKLTYKASGVDIRAGDLAVKKIKKLARSTFTAGVLTDIGLFA
                     GFFSLGKYKMKKPTLVSSIDGVGTKLKVAFMMNKHNTVGEDLVSHCVNDILVHGAKPL
                     FFLDYIACGKLSLGVVNEIVKGLATGCRKNGCALIGGETAEMQDFYKKGEYDLAGCIV
                     GLVEKNRIINGRNIKPTDQIIGLGSNGLHTNGYSLARKVIFGLKRMEATDYVPELKDT
                     IGNQLLKTHRCYAKPIHRILEKYEIKGMAHITGGGIEGNLVRILPQNCKALIDTKNWK
                     VPELFKFIQKWGKVEKSEMYKVFNMGIGYVLVVSKRDTDKILSELASIKEKGYLIGEM
                     VRGKKEVELTNYNAIPTKQRLHNIIKR"
     gene            complement(2263..2928)
                     /locus_tag="VGB16_04245"
     CDS             complement(2263..2928)
                     /locus_tag="VGB16_04245"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_005810750.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="HD domain-containing protein"
                     /protein_id="HEX9916927.1"
                     /translation="MKIVTLEDVKKNPKVELLIKKTDQVLEVIGYTEHGQRHASLAAN
                     IAYNIMTHLGRPEREAHLASIAAYLHDIGNIINRDYHAQTGAMIAYQTLSEMNMCEED
                     IMEIVSAIGNHDEKDGQPVSGICAAVILADKSDVHRSRVRTLDMIGQDIHDRVNYAAK
                     SSFLRVDAEKKVISLEITIDTSISRVMEYFEIFLSRMMVCRKAAEFLKMKFELEINGN
                     RLL"
BASE COUNT          769 a          746 c          542 g          916 t
ORIGIN      
        1 gtcagattat caggtctagg aaactcgtcg aaatcactat cagtcaaagc ccaagacaat
       61 tgtctggctc tatccgccct gaaattggag aatatagcaa catcaccttt ctggataatt
      121 ccactggggt ctatgattgt cggctcaaca aattcatctg tcaccttctt ggcataggat
      181 tcctttatag ccagaattgg gtcgtcattc tttacgcctt cgccaaaaac catggctcga
      241 taggccctct ctatcctctc ccacctctta tcccggtcca tagcgtaata tcttccagag
      301 atggtggcta ttttccctac tttatactcg tccagcttgt cttgtaactc cttgatatat
      361 tttaccccgt tggtaggcga ggtatccctt ccatccagga aagcttggat ataaactttt
      421 ttgaggtcat tagttttcat cagtcttaac agggcataaa gatgctccgg agaactatgc
      481 acgcagccat ccgaaaccaa acctaaaaga tgaagcttgg aattgttttt cttggctata
      541 tcaattgctt caagaaaagc ttggttctta aagaatgaac catcctcgat agctttatct
      601 attcgggtta tctcctgata gactatcctg cctgcaccga tattcaaatg tccaacctca
      661 ctattaccca tcaatccctt gggcaggccg acatccaatc ctgaagcgct tagtgtggta
      721 taaggaaagt tcgagaatat attgtctaaa ttcggtttct tagccaaggc tatggcattt
      781 ccattctttt cttctcttat cccatagccg tccattatca gaagcagaag catatcattc
      841 ctttagtttg tcaactaata caaacgcatt aatttacgtt caataaatct cccctcgccc
      901 tcgcctcgat tcggcccgcc tcggcgagcc atcggcgaga cgggcgaagc gggcctcttt
      961 ttcaaagagg gggaaaagag gtctccccct ttgaaaaagg gggatttgat ggttcgatat
     1021 actcaccatc ctgagcaaag tcgaagggag ggggatttta aaatagattg catattgctc
     1081 taatatgtaa caacatttaa tgcgtttgct ataattagac ccctggccca tcttgcctgc
     1141 ccggccaggg agggcaagat gggtttaccg tttgattata ttatgcaacc tttgtttagt
     1201 aggtatagca ttatagttcg tcaattcaac ttccttcttg cctctaacca tctcaccaat
     1261 taaataacct ttctctttta tgcttgcaag ttcgcttagt attttatccg tatctctctt
     1321 tgagaccacc aaaacatatc ctattcccat attgaagact ttatacatct cactcttttc
     1381 aacttttccc cacttctgaa taaacttgaa gagttctggc actttccaat tcttggtgtc
     1441 aattagagcc ttacagtttt ggggcaatat cctcaccaga tttccttcta ttccgccgcc
     1501 ggttatatgc gccatacctt ttatttcata cttctccaaa atcctatgta tgggcttagc
     1561 ataacatcta tgagttttca agagctgatt accaatcgtg tcctttaatt ccggaacata
     1621 gtcagttgct tccattctct tcaaaccaaa aatcactttg cgagccaaag agtagccatt
     1681 ggtatgaaga ccattagacc ccagacctat gatttggtcg gtcggcttga tgtttcttcc
     1741 atttatgatt ctattttttt caaccaaacc cactatacat ccagccaggt catactcacc
     1801 ctttttgtaa aagtcctgca tctctgcggt ttctccacca ataagagcgc atccgttttt
     1861 cctgcaaccc gtggctaaac ccttaactat ctcgtttact actcccagag agagcttccc
     1921 gcaagcgatg taatccaaaa agaaaagtgg tttagccccg tgcaccagaa tgtcgttcac
     1981 acaatgggat accaaatcct ctcccactgt attgtgtttg ttcatcataa aagccacttt
     2041 gagtttggtt cccacaccat cgatgctgga aaccaaggtt ggctttttca ttttgtattt
     2101 acccaaagag aaaaaaccgg caaaaagccc tatatccgtc aaaacaccag cagtgaaagt
     2161 agagcgcgcc agtttcttta tttttttgac agctaaatct ccggctcgga tatcaacgcc
     2221 ggaagccttg taagttaatt ttctcttcat ctattgaagg ggttaaagca ggcgatttcc
     2281 attaatttca agttcgaatt tcatcttcaa aaactcagct gctttgcggc agaccatcat
     2341 gcgtgacaag aagatctcaa aatattccat cacccgagaa atagaggtgt caatggttat
     2401 ctccagggat atcaccttct tttccgcatc cactctcaga aaggagcttt tggctgcata
     2461 atttaccctg tcgtgaatat cctggccaat catatctaaa gtccttaccc gagagcggtg
     2521 cacatccgat ttgtccgcca agatgaccgc ggcacagata ccgcttaccg gctggccatc
     2581 cttctcgtca tgattcccga tagctgagac tatctccatt atatcctctt cgcacatatt
     2641 catctcggat aaggtctgat aagctatcat cgccccggtc tgagcatgat aatcccggtt
     2701 tatgatgttc ccgatatcgt gtaaataggc ggctatggaa gctaaatgcg cctccctttc
     2761 cggacggccc agatgggtca taatgttata ggcgatattg gcggctaaac tggcgtgtct
     2821 ttggccatgc tcagtatacc cgataacttc caatacctgg tccgtcttct tgataagaag
     2881 ctctaccttg gggttcttct ttacatcctc taaggtaact atcttcatct cttttccttg
     2941 agtttaaatt ttaaacctaa caatataact ggc
//
LOCUS       DASWLN010000128         5490 bp    DNA     linear   ENV 20-FEB-2024
DEFINITION  MAG TPA_asm: Candidatus Zixiibacteriota bacterium isolate
            SMAG_U2949 k141_615555, whole genome shotgun sequence.
ACCESSION   DASWLN010000128 DASWLN010000000
VERSION     DASWLN010000128.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37960594
            Sequence Read Archive: SRR10376091
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Candidatus Zixiibacteriota bacterium (soil metagenome)
  ORGANISM  Candidatus Zixiibacteriota bacterium
            Bacteria.
REFERENCE   1  (bases 1 to 5490)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 5490)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (26-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/12/2024 08:28:26
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 1,440
            CDSs (total)                      :: 1,416
            Genes (coding)                    :: 1,412
            CDSs (with protein)               :: 1,412
            Genes (RNA)                       :: 24
            rRNAs                             :: 1 (16S)
            partial rRNAs                     :: 1 (16S)
            tRNAs                             :: 21
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 4
            CDSs (without protein)            :: 4
            Pseudo Genes (ambiguous residues) :: 0 of 4
            Pseudo Genes (frameshifted)       :: 0 of 4
            Pseudo Genes (incomplete)         :: 3 of 4
            Pseudo Genes (internal stop)      :: 1 of 4
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..5490
                     /organism="Candidatus Zixiibacteriota bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_615555"
                     /isolate="SMAG_U2949"
                     /isolation_source="soil"
                     /db_xref="taxon:2053527"
                     /environmental_sample
                     /country="USA: Oregon"
                     /lat_lon="42.99 N 123.63 W"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            <1..1724
                     /gene="lon"
                     /locus_tag="VGB16_04250"
     CDS             <1..1724
                     /gene="lon"
                     /locus_tag="VGB16_04250"
                     /EC_number="3.4.21.53"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011937627.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004252 - serine-type endopeptidase
                     activity [Evidence IEA];
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_function: GO:0016887 - ATP hydrolysis activity
                     [Evidence IEA];
                     GO_process: GO:0006508 - proteolysis [Evidence IEA]"
                     /codon_start=3
                     /transl_table=11
                     /product="endopeptidase La"
                     /protein_id="HEX9916928.1"
                     /translation="NREVEVLELSRKIQSQAATEMGKMQREFVLREQLKAIKKELGEI
                     DEKTAEIDELSKKIEQAKMPELALDEAKKELDRLSKMNPAAAEYTVSRTYLDWLVSLP
                     WSVSTTDRLDLKQARKVLDEDHYNLEKVKERILEYLAVRKLKTNLKGPILCFVGPPGV
                     GKTSLGMSIARALGRKFNRISLGGMRDEAEIRGHRRTYIGALPGRIIQGIKRCGSNNP
                     IFMLDEIDKIGQDFRGDPSAALLEVLDPEQNFSFSDHYLDVPFDLSKVMFITTANILD
                     PIPPVLRDRTEVIELPGYTDLEKMMIAKQFLIPKELNEHGLSKAYIRFQDSAIKRIIG
                     DYTKEAGLRNLDREIATICRKVAKHVASGNKKKVTVTPDKLQKLLGPIKFFREKTLKR
                     SKVGVVPGLAWTQVGGEVLFVEATKMPGRKSLTLTGNLGEVMKESVQTALSYVRSRSD
                     RLGLPKNFYDRLDIHVHVPSGAIPKDGPSAGVTMAAALASLLTNQKVKPKLAMTGEIT
                     LRGDVLPIGGLKEKTLAAYRAGIETVILPQDNRKDMVEIPKEIKEKVKFVFAATMDEI
                     LEKALEK"
     gene            1767..3209
                     /locus_tag="VGB16_04255"
     CDS             1767..3209
                     /locus_tag="VGB16_04255"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_012421020.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="tryptophanase"
                     /protein_id="HEX9916929.1"
                     /translation="MLVRLSNGKEIPMEMHKVRIVQKTYLVPIDQRLKAIEEGGYNTF
                     SLRTKDIFMDSLTDSGTNAMSDNQLAAMMVSDDAYAGSESFYRLASSIKDVLGFDYVL
                     PVHQGRAAEHLIAKVFVKAGDVIPMNYHFTTTKAHFELAGGTVLEIYRDLALQTESVE
                     PFKGNLDLQKFKDVIKKYGRDKISFVRMEATANLLGGQPFSMENLREVKKIASENGIF
                     LVYDGSLISENAYFIKKREKGYENKTIGEIIHEMMSYVDLFYLSGRKSTCVRGGFIAT
                     NHKKYFEMIKPWLPVYEGFLTYGGMSTKEIEAMAVGLREMTDMNVAGSAAEFVKYFAE
                     RLIETKIPVVTPAGGLACHLDAKKFLPNMPQSQYPAGALAAAIYITSGVRTMERGTIS
                     MDRDKDGNEVYSDLELARIAIPRRVYTMSQIEYAVDRLNWLYKHRDLIGGLKFIEEPP
                     VLRFFFGKLKALDNWGAKLAEVFKKEIGTL"
     gene            3995..>5490
                     /gene="amrB"
                     /locus_tag="VGB16_04260"
     CDS             3995..>5490
                     /gene="amrB"
                     /locus_tag="VGB16_04260"
                     /inference="COORDINATES: protein motif:HMM:TIGR04336.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="AmmeMemoRadiSam system protein B"
                     /protein_id="HEX9916930.1"
                     /translation="MKKILIIALVISLGVASNCGNSSSQTEDNKMTQEIRKPAVADAF
                     YPANPVALSQMIADFFKRVKKEEIGGEIVAIISPHAGYIYSGQVAAYGYKMLEGMKFE
                     TVVMIAPNHIFPFAGASVYNGAFYETPLGKIECDQAVAKKIASYHKSVYLSDKGHSFY
                     GGRGEHSLEVQLPFLQIVLGKFKLVAIIMGDQEYSTCEKLGDAVSKALKDKPALIVAS
                     SDLSHFHPYSEAVKLDQVVVGDVNSFDPQGLFDDVSKGKCEACGAGPIVATMLAAKKL
                     GADKAKVLKYANSGDVTGDKSSVVGYMSAVIYRSGEGPTQEEKEEKSKKVGVDMGLSQ
                     SDEEVLLNIARKTVEARVKGQRPPEFKVTSEILKENRGAFVTLEKNGQLRGCIGYIQA
                     IKPLYITIEEMAEEAALSDPRFAPVSEEELNDLHIEISVLTPLKKISDISQIEVGRDG
                     IYIKKGFNSGLLLPQVATEYGWDRETFLEQTCYKAGLPPNAWKEKTCE"
BASE COUNT         1591 a         1102 c         1465 g         1332 t
ORIGIN      
        1 ttaaccgtga agttgaagtg ttggagcttt cccgcaagat tcaatctcag gccgcaactg
       61 agatggggaa gatgcagagg gaattcgttt tgagggagca gctcaaagcc ataaagaagg
      121 agttaggaga aatagatgag aagaccgcag agatagatga gttgagcaag aagatagagc
      181 aggccaagat gccggagttg gctttagatg aggctaagaa ggagttagat agattatcca
      241 agatgaatcc agctgccgcg gaatatactg tgtcccgaac ttatctggat tggttggtta
      301 gtcttccttg gagcgtaagc acgactgacc gtttggacct gaagcaagcc agaaaagttc
      361 tggatgagga ccactacaac ctggaaaaag tcaaggaaag gatattggaa tatttagcag
      421 tgagaaaact aaaaaccaac ctcaagggtc caattctttg ctttgttggt cccccaggtg
      481 ttggtaagac ctccctgggt atgtccattg cccgtgccct gggaagaaaa ttcaatcgca
      541 tctctctggg tggaatgagg gatgaagcgg agataagggg acacaggaga acctatattg
      601 gggctctgcc cggaaggata attcagggaa tcaagagatg cggctccaac aacccgatct
      661 tcatgttgga tgagatagat aagatcggtc aggatttcag aggagacccg tctgccgccc
      721 ttttggaggt tctggaccca gagcagaact ttagcttctc cgaccattat ctggatgttc
      781 cttttgatct gtccaaagtg atgtttatca ccacggctaa cattctcgac ccaattccgc
      841 cggtactgcg tgaccggacg gaagtgatcg agctaccggg atatactgat ctggagaaaa
      901 tgatgattgc caaacagttt ctgataccga aagagctgaa tgagcatggc ttgagcaaag
      961 catacatcag atttcaggat agtgccataa agaggataat tggtgattat actaaagagg
     1021 caggtttgag gaatctggac agagagattg ccactatctg ccgtaaggtg gcaaagcatg
     1081 tcgcttccgg caataaaaag aaggtgacag tcaccccaga taaactgcaa aagcttcttg
     1141 gaccgattaa gtttttccgc gagaaaactc tcaagagatc gaaggtgggg gttgtccctg
     1201 gtttagcctg gacacaagta ggaggagagg ttcttttcgt cgaagccacc aagatgcctg
     1261 gcagaaaatc tctgacctta accggaaatt tgggcgaggt gatgaaggaa tcagtccaga
     1321 cagctctctc ttacgtccgt tcgcgaagtg acagattggg tttgcccaaa aatttctatg
     1381 atcgattgga cattcatgtt catgttccgt ctggcgccat ccccaaggat ggcccctcag
     1441 ctggggtaac tatggcagct gccttggctt ctcttttgac taaccaaaag gtcaagccca
     1501 aattggctat gaccggagag ataaccttgc gcggggatgt gcttcccatc ggaggtctaa
     1561 aggaaaagac tctagccgcc tatcgggcag gaatcgaaac agtgattttg ccccaagaca
     1621 atcgaaaaga tatggtggaa atccccaagg agataaaaga aaaggtaaaa ttcgttttcg
     1681 ccgcaactat ggatgaaatc ttggaaaaag ctttggaaaa gtaaaggcaa atggttttaa
     1741 aaccccaaac caataaggag gtttaaatgc ttgtcaggct tagcaacggg aaagagattc
     1801 ccatggagat gcacaaggtg aggatcgttc agaagaccta cctggtgccg attgatcaga
     1861 ggttgaaggc catagaagaa gggggataca ataccttctc gcttcgcacc aaagatattt
     1921 ttatggattc gctgaccgac agcgggacca acgctatgag cgataaccag ctggcagcta
     1981 tgatggtttc ggatgatgct tatgccggaa gcgagagctt ttatagatta gcttcatcta
     2041 tcaaggatgt tctgggcttt gactatgtgt tgcccgtgca tcaaggaaga gcagcggagc
     2101 acctaatagc caaggtcttt gtcaaagcgg gtgatgttat cccgatgaac tatcatttca
     2161 ccaccaccaa agcccacttt gaattggctg gtggcacggt tttagagatt tatcgggatt
     2221 tggctttgca gactgaaagc gttgagccat ttaagggaaa tctagacctt cagaaattta
     2281 aggatgtcat aaaaaagtat ggaagagata agatctcctt cgtgcgcatg gaagcgaccg
     2341 ccaatctctt gggaggacaa cccttttcca tggagaattt aagagaggtg aagaaaatcg
     2401 cctcggagaa tggcatcttt ttggtgtatg atggaagcct gatctcggag aatgcctatt
     2461 tcatcaagaa gagggaaaaa ggatacgaga acaagaccat tggtgagata atccacgaaa
     2521 tgatgagcta tgtggatctc ttctatcttt cgggcagaaa gagcacctgc gtcagaggcg
     2581 ggtttatcgc caccaaccac aagaagtatt ttgagatgat aaaaccctgg cttccggtgt
     2641 acgaagggtt cttgacctat ggcggaatgt caaccaaaga gatcgaagcc atggctgtgg
     2701 gcctccggga gatgaccgat atgaatgtgg cggggagtgc agcagagttt gtgaaatatt
     2761 ttgctgaaag attgattgaa accaaaatcc ccgtggttac cccagccggt ggattagcct
     2821 gccatctgga tgcgaagaaa ttcttgccca atatgcctca atctcaatat cccgccggag
     2881 cattggctgc ggcaatctac atcacctctg gagtcaggac catggagcgg ggcaccatct
     2941 ctatggatag agataaagac gggaatgaag tttattccga tctggagtta gctcgtatcg
     3001 ccattccccg aagagtctac actatgtccc agatagaata tgcagttgac cgtttgaatt
     3061 ggctttacaa acatcgcgac ctgattggtg gattgaaatt catcgaggag ccgccggtat
     3121 tgaggttctt ctttggaaag ttgaaagcgc tggataattg gggcgccaag ttagctgagg
     3181 tttttaagaa ggaaataggc actttgtaat aaaggtgatt aagacaagta gtctgatata
     3241 atatagaagt aggtcgggct gtcccgccaa ggtgggacag cccgacaatg aagctgtcat
     3301 tgaggctgtg ttctaatgtc attctgaggc gttagccgaa gaatctcgat tttctaactt
     3361 atctcaagat gcagaattcg cactgaaact attagattct tcccccgcca aggcggggtc
     3421 agaatgacaa ccgcgtgttt ttaagtgttg cgacatagtc tgattgcgag gaatgtagta
     3481 acgaagcagt ctaatttatt gtaggagcga cttaggattc tgtaggggag ccccttcctt
     3541 cgacttcgcc cttcggtctg agctcagggt cgaagactca ggacggtgag cttgtcgaac
     3601 cgcgtgggct cccgtaagag tctaagacta aagtcgatta gagcaactaa acaacgggag
     3661 gggacaagcc cctcccctac gaaaaactat aagaatcagg gtatttttct gttggttcgg
     3721 gaattgtcac taggatcccg cttggcggga gatttcccga accatatcac gtgcgggaat
     3781 tcgccggcat gggccttgat aacaattccc gcacaaacag agtgaagtgg ggagaaaata
     3841 gaaccccctt ttgtatcccc ccttgtcagg ggggaaaaga aaaacaagtc cccctgataa
     3901 gggggattta gggggttccc aggcacacaa acaggccttt tctctcttcg ctatttttca
     3961 aagaaagaaa gttaattcga tgctggaaat taaaatgaag aagatactga ttattgcact
     4021 tgtaatctcg ttgggcgtag cttcgaattg cggcaattcc tcatctcaaa cggaggataa
     4081 taagatgaca caggaaataa gaaaaccagc tgtggcagat gcattttatc cggctaatcc
     4141 agttgccctc tctcaaatga tagctgattt tttcaaaaga gtgaaaaagg aagaaattgg
     4201 aggtgaaatt gtagccataa tctctcccca tgccggatat atctattccg gacaggtggc
     4261 agcatacggt tacaaaatgt tggagggtat gaaatttgag accgtggtga tgatcgctcc
     4321 caatcatatc ttcccctttg ctggcgcctc agtttataat ggggcttttt atgaaacccc
     4381 tctggggaaa atagagtgtg accaggcagt tgccaaaaaa atagccagct accataaatc
     4441 agtttatctt tcagataagg gacattcttt ttacggagga agaggagagc attctctgga
     4501 agtgcaactg ccttttttgc agatagtttt gggcaaattt aagttggtgg ctatcataat
     4561 gggagaccag gaatactcaa cctgtgagaa gctgggagat gcggtatcca aagcactgaa
     4621 agataagccg gctttgattg ttgccagctc ggacttatcc cattttcatc cttatagcga
     4681 ggcagtgaaa ttggaccagg tggtcgtggg cgatgttaat tcgttcgacc cccaagggct
     4741 ttttgatgat gtttccaagg ggaaatgcga agcgtgtggg gcaggtccca tagtggcaac
     4801 catgcttgcg gcaaagaagc ttggagcaga caaggcaaag gttttgaaat atgccaattc
     4861 cggagatgtg acgggtgata aatcgagcgt ggtgggatat atgtcagctg taatctatag
     4921 aagcggagag ggtcccacac aagaggaaaa ggaagaaaaa tccaaaaaag taggggtaga
     4981 tatggggcta tcgcagagcg atgaagaggt gcttttaaat atcgcccgta agaccgtcga
     5041 agccagagtt aaaggtcaaa ggccgccgga gtttaaggtc acctcagaaa tcctaaaaga
     5101 gaacagagga gcttttgtca ccttggagaa aaatggacag ctgcgcggat gcataggtta
     5161 catccaagcc ataaagccac tttatatcac gattgaggaa atggcggagg aggcagcttt
     5221 aagtgacccg agatttgctc cggtatcaga ggaggagtta aatgatttgc acattgagat
     5281 atcagtacta accccgctca agaaaatctc cgatattagc cagattgaag ttggcaggga
     5341 cggtatttat atcaaaaagg ggttcaattc cggcttgctt ctcccgcagg tggcaacgga
     5401 atatggatgg gacagggaga catttttaga gcagacctgt tataaggcag gattgccgcc
     5461 caatgcctgg aaagagaaaa cctgcgagat
//
LOCUS       DASWLN010000129         9494 bp    DNA     linear   ENV 20-FEB-2024
DEFINITION  MAG TPA_asm: Candidatus Zixiibacteriota bacterium isolate
            SMAG_U2949 k141_359739, whole genome shotgun sequence.
ACCESSION   DASWLN010000129 DASWLN010000000
VERSION     DASWLN010000129.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37960594
            Sequence Read Archive: SRR10376091
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Candidatus Zixiibacteriota bacterium (soil metagenome)
  ORGANISM  Candidatus Zixiibacteriota bacterium
            Bacteria.
REFERENCE   1  (bases 1 to 9494)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 9494)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (26-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/12/2024 08:28:26
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 1,440
            CDSs (total)                      :: 1,416
            Genes (coding)                    :: 1,412
            CDSs (with protein)               :: 1,412
            Genes (RNA)                       :: 24
            rRNAs                             :: 1 (16S)
            partial rRNAs                     :: 1 (16S)
            tRNAs                             :: 21
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 4
            CDSs (without protein)            :: 4
            Pseudo Genes (ambiguous residues) :: 0 of 4
            Pseudo Genes (frameshifted)       :: 0 of 4
            Pseudo Genes (incomplete)         :: 3 of 4
            Pseudo Genes (internal stop)      :: 1 of 4
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..9494
                     /organism="Candidatus Zixiibacteriota bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_359739"
                     /isolate="SMAG_U2949"
                     /isolation_source="soil"
                     /db_xref="taxon:2053527"
                     /environmental_sample
                     /country="USA: Oregon"
                     /lat_lon="42.99 N 123.63 W"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            <1..226
                     /locus_tag="VGB16_04265"
     CDS             <1..226
                     /locus_tag="VGB16_04265"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=2
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEX9916931.1"
                     /translation="INLPSPSPRFGPPRRAIGETGEAGLFSKEGKKEVSPFEKGGFDG
                     SIWASTESFDGEPLKTVEPLSRMSSPSLRA"
     gene            519..3248
                     /gene="ppdK"
                     /locus_tag="VGB16_04270"
     CDS             519..3248
                     /gene="ppdK"
                     /locus_tag="VGB16_04270"
                     /EC_number="2.7.9.1"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_018205212.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0050242 - pyruvate, phosphate dikinase
                     activity [Evidence IEA];
                     GO_process: GO:0015976 - carbon utilization [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="pyruvate, phosphate dikinase"
                     /protein_id="HEX9916932.1"
                     /translation="MKKSPKRDSPKKKVVFGRSKKQFYFFGEGKADGNGKMKDVLGGK
                     GAGLAEMTRIGVPVPAGFTISTEVCSLFYKNRMKLPKQFDEEMKKHIKKIENCMGAKF
                     GDPKDPLLLSVRSGAKFSMPGMMDTILNLGLNEKTVLGLIKKTKNERFAYDSYRRFIQ
                     MFGNVVLGIDKKEFEQLIQRKKEKKDVKLDTSLLVEDLKEIIASFKKLIKSKSGEDFP
                     QDPVKQLQMARDAVFKSWNNPRAITYRRINSIPADLGTAVNIQAMVFGNMGENSCTGV
                     GFTRNPSTGDNKFYGEYLTNAQGEDVVAGIRTPKPIDQLAKEMPKAYQRLREITTRLE
                     KHYRDVQDFEFTVQEGKLYMLQTRTGKRTTYAAVKIAVDMVKEKLIDKKEALSRIEPE
                     QLNQLLHRRIDPSAPVNVIAKGLAASPGAASGKVIFTAEDAIKLHQAKEKVVLVRQET
                     NPDDIHGMEVAEGILTARGGMTSHAAVVARGMGKCCVAGCEEVRVNEQRKQFTVKGQI
                     IRERDIITLNGSTGEVILGEVPTIEPELSGEFGEFMGWADGIRRLGVRTNADLPRDAI
                     TAVKFGAEGIGLCRTEHMFFAEERLPIVQEMILALTKEDRQIALDKLLPFQKADFKGI
                     FEAMNGRPVTIRTLDPPLHEFLPKKEDILEEIKELKASPEQSEEIERKVKEKERVLDR
                     IEELAEFNPMMGLRGCRLGIVYPEITEMQARAIFEAACELTRGGKKVLPEVMIPLVGE
                     IKELENQKAVVVKVAEETMKKYKVKIKYLVGTMIEIPRATVTADKIAQVADFFSFGTN
                     DLTQMGFGFSRDDAGKFLKFYVEHGILPKDPFVSIDREGIGEFIRIGVANGRKTNPKL
                     KIGICGEHGGDPDSIHFCDEVGMDYVSCSPYRVPIARLAAAQAVLGKSEYTSK"
     gene            3445..4608
                     /locus_tag="VGB16_04275"
     CDS             3445..4608
                     /locus_tag="VGB16_04275"
                     /inference="COORDINATES: protein motif:HMM:NF012382.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0030170 - pyridoxal phosphate binding
                     [Evidence IEA];
                     GO_process: GO:0009058 - biosynthetic process [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="aminotransferase class I/II-fold pyridoxal
                     phosphate-dependent enzyme"
                     /protein_id="HEX9916933.1"
                     /translation="MKNRFPNRLMKLDDYIFSQIKETKRLTSDLEPIDLSLGNPDDPP
                     DREIIRTLIREARVLENHRHPLYRGIPELKEKIAEWFFKRFKVKLNPETEILILTGSK
                     QGLGLVGLTFLNKGDVALVPNPAYPVYRRGVLLAEGKPLDMPLLRENRWLPDLDKLSK
                     KISSKTKIIFINYPNNPTGAVADISFLKKSVDFAVKNDIIIISDMAYSEITFDNFKTH
                     SIMEIDGAKQRTLEFFSFSKSFNMTGWRLGFVVGNRDLINALQKIRVTLDSGVFHLVQ
                     KGGIAALSHPEKTKNVCQIYQRRRDILFNGLKDCGWEVEKPKGAIFIWAKPPWQCSSS
                     QSVSILKKKAGIITTPGTGLGSQGEEYIRFSLTAPDEKIKQVVKRLKELPIGR"
     gene            4641..5366
                     /locus_tag="VGB16_04280"
     CDS             4641..5366
                     /locus_tag="VGB16_04280"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEX9916934.1"
                     /translation="MKKMSVFLVVGLCLLLFTGCANKPVSPVSTYKFTIDGVLVKDMA
                     SGYDVAWFRVLKDSLAYDSAIITVDSDTVPNFIGHNYMNFDVVLKPESTYTVKVSCPN
                     YDFSLTKYIIIPDTFHISIIDPVVNRGGQQTTISWTASQYASGYFVIVSPPSPEAVGH
                     SALVNSSPETVDPPAFRTTSQDRVIYGTYLVYVVSYRESYLLYNYLFSIPDSLPTGNL
                     NGANGTIGAGVIAPEGFIVVTTL"
     gene            5363..6295
                     /gene="murQ"
                     /locus_tag="VGB16_04285"
     CDS             5363..6295
                     /gene="murQ"
                     /locus_tag="VGB16_04285"
                     /EC_number="4.2.1.126"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_014560164.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016835 - carbon-oxygen lyase activity
                     [Evidence IEA];
                     GO_process: GO:0006040 - amino sugar metabolic process
                     [Evidence IEA];
                     GO_process: GO:0009254 - peptidoglycan turnover [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="N-acetylmuramic acid 6-phosphate etherase"
                     /protein_id="HEX9916935.1"
                     /translation="MSRKVFNQIKDLVTEARNPDTKRIDSLSVLQILKLINHEDKKVA
                     SSVEEVLPQIAKGVDLIVNSLKNKGRLFYIGAGTSGRLGVLDAAECPPTFGTSPDMIK
                     GIIAGGKRSLILSREGAEDNKKAGRLDIRKNKVRKKDVVIGIAASKRTPYVLFALKEA
                     KKIGAKTIFIFCNPISSTNIKVDVAISPVLGPEVIMGSTRMKAGTAQKMILNMLSTAS
                     MIRLGKVYENMMVDLRATSQKLIERSKRIVMLATGVNYKKAEAYLNKADGHVKTALVM
                     ILAKADKTEAKERLKKTQGFVREAIQSKYVRRKR"
     gene            6276..6527
                     /locus_tag="VGB16_04290"
     CDS             6276..6527
                     /locus_tag="VGB16_04290"
                     /inference="COORDINATES: protein motif:HMM:NF020967.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF2007 domain-containing protein"
                     /protein_id="HEX9916936.1"
                     /translation="MSEEKDKEFIDYVPFRTFPSRLYAEMLKEALARQGIPSIIKGDD
                     IGIMLGSYSTTSLVEVTLWVRKEDKSQCEEIADFMLDHI"
     gene            6821..8644
                     /locus_tag="VGB16_04295"
     CDS             6821..8644
                     /locus_tag="VGB16_04295"
                     /inference="COORDINATES: protein motif:HMM:NF025030.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="HEAT repeat domain-containing protein"
                     /protein_id="HEX9916937.1"
                     /translation="MPEVEPQEKLEEETEEIDQDELSEVEKLIQFFLKTVKSMVLYPE
                     ENPIPKEFRRDLYDRFSQLLEKYDQLNLKVNETQLIYKGKMVYRDGGKDERLAHFLYR
                     DGIREITFFKGLEQRELFDFLDAMRTVSRTGEMEDDLVTILWEKDLINVSYVVIEDWG
                     EQGPGYQEDLSVTLVTQVIPQYKKGSTDFDFQEIYSSELASPGTVVNDETEQAISSWE
                     KHTTQGLAGVTFLQKSADLSRILANLDRFSAEEVGEINKILEMDRTYVPFDALLSVLY
                     ETLYWEDEQSAFTEILREVEGMLHTYLNKADFSCASRMVTLFRDLEQEFKGICSFKAN
                     SIKEALDRAGDKERIRYIINALNDRKDMELSAVEHYLTLLNSNVVSSLIEMLRHLRFF
                     PARKMVCEVMGYIGKDHVQMVGEGVYDPQWYVVRNIAIVLGKIGKLECVEFLKAAIVH
                     ADARVRREAVRSLTGIDSQEAGEVLLLSLNDSYNIIRSIGIRALSQRGDAAAVKPLMQ
                     ILQDKKFRERSSEERKLMLDALAHLGRDRSIPVLVELINKRSWFDRVRNNETRLFALR
                     ALGLVNTLASYQALDELGHKGDKLVRQSCSAILRKREHKSY"
     gene            8650..>9494
                     /locus_tag="VGB16_04300"
     CDS             8650..>9494
                     /locus_tag="VGB16_04300"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEX9916938.1"
                     /translation="METQTDKKPRKNPSRENPKVIDRILNEMGKSLVNQLYATFKTAQ
                     IYERNNLAFVKQSEALISITYSILEREGEIHLRSKSGYLFLNRSRLIFNFEGYVSSRF
                     IMDEFKRCQMESIFIENGVTKREMDEFISQFVHLDKDKEDALEALEDRLNQLEVHHVR
                     VERIRPDWIRPKTHQVAFSDMRKEAKHTFFKAISVAQEIMTSVKSGRAVNTVKAKRVI
                     QSLVDQIIQDESSFLELTAIKNYDEYTFAHSVNVCVLAIAFGLKLGLDRKKLSELGFS
                     ALFHD"
BASE COUNT         2937 a         1765 c         2374 g         2418 t
ORIGIN      
        1 aataaatctc ccctcgccct cgcctcgatt cggcccgcct cggcgagcca tcggcgagac
       61 gggcgaagcg ggcctctttt ccaaagaggg gaaaaaagag gtctccccct tcgaaaaagg
      121 gggatttgat ggttcgatat gggcttcgac tgagtccttc gacggtgagc cgctcaagac
      181 ggtcgaaccg ctcagccgaa tgtcctcacc atctcttcga gcctgagcct tccttcgaca
      241 ctgaggctca ggacgaagtg cagagccgaa ggcctgagca aagtcgaagg gaggggaatt
      301 ttaaaataga ttgcatattg ctctaatata taacaacgtt taatgcgttt gctatagttc
      361 agggttaact aactggtaaa ctcacccctc tgttttaaaa gcagaggggt gatctgtttt
      421 tgccctgctt tttcaaggaa tgagaatttg cttgcttttt cgaagagaat aaatataatt
      481 tagggaaagt tttttcgaaa atacctaagg agggatagat gaagaagtct cctaagagag
      541 attctcccaa gaagaaagtg gtttttggca ggagcaaaaa acagttctac ttttttggag
      601 agggcaaggc agacggaaat ggcaaaatga aggatgtact tggaggaaag ggagctggtt
      661 tggctgagat gaccaggatc ggagtgccag tcccagctgg atttaccata agcacagagg
      721 tttgtagtct cttctacaag aacaggatga agttgccaaa acaatttgac gaggagatga
      781 aaaagcacat taagaaaata gagaactgta tgggggcaaa attcggtgat cccaaggacc
      841 cgcttttatt gtcagtgcgg tctggtgcca aattttcaat gccggggatg atggatacta
      901 ttctcaactt aggtttgaat gagaaaactg ttttgggact aatcaagaag accaaaaatg
      961 aaaggttcgc ctacgatagc tatcgccgct ttattcagat gtttggaaat gtggttttag
     1021 gcatagataa aaaggagttc gagcagctaa tccaaaggaa aaaagagaag aaagatgtaa
     1081 aattggatac ctcacttcta gttgaagacc taaaggagat cattgccagc ttcaagaaat
     1141 taatcaagag caagagcggt gaggattttc ctcaggatcc tgtcaagcaa ttacagatgg
     1201 cacgagatgc ggtgtttaaa tcctggaata acccccgggc tataacttat cgccgcatta
     1261 atagtatccc tgcggatttg ggcactgcag tcaatattca ggcaatggtc ttcgggaata
     1321 tgggagaaaa cagctgtacc ggcgtgggat ttactcgtaa cccctcaact ggagataaca
     1381 aattctatgg agaatatcta accaatgcgc agggggaaga tgtggtggct ggcattagaa
     1441 ctcccaagcc gattgaccag ctggctaagg agatgccaaa agcctatcaa cggcttcgag
     1501 agataaccac cagattggaa aagcattaca gggatgtgca ggattttgag ttcacggtgc
     1561 aggaaggaaa actgtatatg ctccagaccc gaaccggaaa gagaaccacc tatgcggctg
     1621 ttaagatagc ggtggacatg gtgaaagaaa agttgattga taagaaggaa gctttaagcc
     1681 ggattgagcc agaacagttg aaccagctcc tgcatcgcag aattgatccg agtgccccgg
     1741 tcaacgtcat agccaagggg ttagccgctt cacccggagc agcttcaggt aaagtgattt
     1801 tcactgcgga agatgcgata aaactgcatc aagccaagga gaaggttgtt ttagttagac
     1861 aggagactaa cccggacgac attcacggca tggaagtagc tgaggggata ttaaccgcgc
     1921 gtggaggaat gaccagtcat gccgcagtgg ttgctcgggg tatgggaaaa tgctgtgtgg
     1981 ctgggtgtga ggaggttcga gtgaacgaac agaggaaaca gtttacagta aaaggtcaga
     2041 taattcgaga gagggatatc atcaccttaa atggctctac cggcgaagtg atattaggcg
     2101 aagtgcctac tattgagccg gagctttcag gtgaatttgg agagtttatg gggtgggcag
     2161 acggaatcag gaggctagga gtgagaacta atgccgacct gcccagagat gccattacag
     2221 cagtgaaatt tggggcagag ggtatcggtt tgtgccgaac agaacatatg ttcttcgcag
     2281 aagaaagact acctatagtg caagaaatga ttctggctct aaccaaggag gacaggcaaa
     2341 ttgccttgga taagcttctg ccttttcaga aagccgattt taaaggaatc ttcgaggcga
     2401 tgaatggtcg gccagtaacc atccgcaccc ttgatcctcc cttgcacgaa ttcttgccca
     2461 agaaagagga tattttagag gaaataaaag agttaaaagc aagcccagag cagagtgagg
     2521 aaatagagag aaaggttaaa gagaaagaaa gggtcttgga cagaatagaa gagctggcag
     2581 aattcaatcc aatgatgggc ctgcgtggtt gtcgtttggg tatagtctat ccagagataa
     2641 ccgagatgca agcccgggca atattcgaag cggcttgcga gttaaccaga ggagggaaaa
     2701 aggtcttgcc agaggtgatg atacctttgg tgggagaaat taaggaattg gagaatcaaa
     2761 aagcggtcgt ggtcaaagta gcggaagaga ctatgaagaa atataaggtc aagattaaat
     2821 atttagtggg gactatgatc gagatacctc gagccactgt cactgcggat aagatcgctc
     2881 aagtggcaga cttcttctct tttggaacaa acgacctcac tcaaatgggt tttggttttt
     2941 cacgagatga tgcgggtaaa ttcttgaagt tctatgtgga gcacgggatc ttgcccaaag
     3001 acccattcgt ctccatagac cgagagggga ttggagagtt catccggata ggtgtggcaa
     3061 acggaagaaa aactaatccg aaattgaaga ttggaatatg tggtgagcat ggaggagacc
     3121 ctgattccat acacttctgt gatgaggtag gaatggatta tgttagttgc tcgccctatc
     3181 gggttccgat tgctcgctta gccgcagccc aagcggtttt agggaaaagc gagtatacct
     3241 caaagtgagt agattagaat tttgactgca gagccgcttt caactgaagg cggctttatt
     3301 tttttgtttg tcattcctgt gctatgaacc ataacggttc atggaacagg aatgacagga
     3361 ggactaagca gtattctgct cttagtaatt tagtctgttt ttatattgac ttcaacattg
     3421 ttttggatgt aatttataac caatatgaag aatagatttc ccaatcgctt aatgaaattg
     3481 gatgactata ttttttcaca aataaaggaa accaaaagat taacttcaga tttagagcca
     3541 attgatttaa gcttgggtaa tccagatgac ccgccagaca gagaaatcat tcgaactttg
     3601 ataagggaag ctcgggtttt ggagaatcat cgccatccgc tttatcgagg tatcccggaa
     3661 ctaaaagaga aaattgcaga gtggtttttc aaaagattta aggtcaaatt aaatccggaa
     3721 actgaaattc tgattttaac tggctcaaaa caaggattgg gactggtggg tttaactttc
     3781 ctgaacaaag gggatgtcgc cttagtgcct aatccggctt atccggttta tcgaaggggt
     3841 gtccttttag cagagggaaa gccattagat atgccgcttt taagagagaa cagatggctg
     3901 ccggatttag ataaattaag caagaaaatt tcctcaaaaa ctaaaatcat ctttataaac
     3961 tatcccaata atccgactgg ggctgtggca gatataagct ttttgaaaaa gtcagtggat
     4021 tttgcagtta agaatgatat tataatcata agcgatatgg catactcgga gataactttc
     4081 gataatttca aaacacatag tatcatggaa attgatggag caaaacaaag aactctggaa
     4141 tttttttcct tttccaaaag cttcaatatg accggctgga gattaggatt tgtggtggga
     4201 aatagagatt tgattaatgc ccttcaaaag ataagagtta cattggattc aggggttttt
     4261 catcttgtac aaaagggagg gatagccgct ttgtctcatc ctgaaaagac aaagaacgtt
     4321 tgtcagattt atcaaagaag aagagatatt ctttttaatg ggttaaagga ttgtggctgg
     4381 gaagtggaga aaccgaaagg ggcaatattc atttgggcta aaccaccttg gcagtgtagt
     4441 tcaagccaat ctgtgtcgat attaaaaaag aaggcgggta taattactac ccccggtacg
     4501 gggctaggtt cacaaggaga agaatatata agattttccc taactgctcc ggatgagaaa
     4561 ataaaacagg tagtaaaaag attaaaagaa ttgccaatcg gtcgataaaa gcattacgac
     4621 ccgtagggag gtaagaaaag atgaaaaaaa tgtcagtttt cttagtggtg ggtttatgcc
     4681 tacttctgtt tacggggtgt gcgaacaagc cggtctcgcc agtgtccact tacaaattca
     4741 ccatagacgg ggttttggtg aaggatatgg caagcggtta tgatgtcgca tggtttaggg
     4801 tgctcaaaga ttcacttgct tatgatagtg ctataatcac tgtagatagt gatactgttc
     4861 ctaatttcat aggtcataac tacatgaact ttgatgttgt acttaagccc gaatcgactt
     4921 atactgtaaa ggtatcctgt cccaattatg atttttcact caccaagtac ataataatac
     4981 cggatacttt tcacataagc atcatagacc cagtagtaaa taggggaggg cagcaaacta
     5041 cgatttcttg gactgcttcc cagtatgctt caggatattt cgtgattgtt tcccccccaa
     5101 gcccagaagc agtagggcac agcgcattag ttaactcttc tccagaaacc gtcgacccac
     5161 ctgctttcag aaccaccagt caggacaggg taatctacgg tacgtacttg gtttatgtgg
     5221 tgtcctacag agaaagctac ctcttatata actatctatt cagcatcccc gatagtttgc
     5281 caactgggaa tttgaatggt gcaaatggca ccatcggagc aggggtgata gcacccgagg
     5341 gtttcatagt ggtgactaca ctttgagcag aaaagtcttc aatcagataa aggatttggt
     5401 taccgaagcc cgcaatcctg ataccaaaag gattgactca ctttctgttt tacaaatcct
     5461 aaagttaatc aaccacgaag ataaaaaagt agccagctca gttgaggagg ttcttcctca
     5521 gatagccaag ggagtggatt tgattgtcaa ctccctaaaa aacaaaggac ggctctttta
     5581 tatcggtgcg ggaacaagtg gcagattagg agttttagat gcggctgagt gcccacccac
     5641 tttcggcact tctcctgata tgattaaagg gattatcgct ggcggaaaaa gaagtttgat
     5701 tttatcgcgc gagggagcgg aggataacaa aaaagctggc agattagata taagaaagaa
     5761 taaagtaaga aaaaaggatg tggtgattgg aattgccgct tctaaaagaa ccccttatgt
     5821 cttgttcgcc ttgaaagagg caaaaaagat tggagcgaaa actattttca tcttctgcaa
     5881 ccccatctca tctaccaaca taaaggttga tgttgctatt tctcctgttt tggggcctga
     5941 ggttattatg ggctccaccc ggatgaaagc tggcactgcc cagaagatga tactcaatat
     6001 gctctccacc gcctcgatga taaggttagg caaggtttac gagaatatga tggtggattt
     6061 aagagccacc agccagaaac taattgaacg ttcaaagagg atagttatgt tagctacagg
     6121 ggtaaattat aagaaagctg aagcttatct taacaaagca gacggtcatg tcaagaccgc
     6181 tttggtgatg atactggcaa aggcggataa aacagaagcc aaagagagac tgaaaaaaac
     6241 ccaaggattt gtaagagaag ctatccaaag taaatatgtc agaagaaaaa gataaagagt
     6301 tcatagatta cgttcctttt cgcacttttc ccagtcggct ttatgctgag atgctaaaag
     6361 aggcgttagc ccgtcagggt attccctcaa ttatcaaagg ggacgatatc ggcataatgc
     6421 tgggaagcta ctccaccacc tctctggttg aagtaactct ctgggttaga aaagaggaca
     6481 aatcccagtg cgaagagata gctgatttta tgctagacca catctaatac ctcttcttta
     6541 aaatcagcag gtcaggagcg cttggctatt gacctaaaca attagcttta tccctgcgat
     6601 attttgcatc ttggaatgta ggggaagact ttggccttcc atttttggaa acctgaacgt
     6661 ttccgctaca taaaacttaa cctcacccta accctctcct tgaaaaggag aggggatttt
     6721 gttcatacgg gcaaaagtgt ctgcttggct aagacttaaa tcttccagtc tagatgtcga
     6781 taataaagaa tgaggtagtc tatttaaaac agggagattt atgcccgaag tcgagcccca
     6841 agaaaagctt gaagaagaga ccgaagaaat agaccaggat gagttaagtg aggttgagaa
     6901 actaatccag ttcttcctca agaccgtgaa atccatggtt ctctatcctg aagagaaccc
     6961 cattcccaag gaatttaggc gcgacctata tgacaggttc agccagcttc tggagaagta
     7021 cgaccaacta aacctgaaag tcaatgagac tcagcttatt tacaaaggaa agatggttta
     7081 tcgggatgga ggaaaagatg agagattggc tcatttctta tatagagatg ggataagaga
     7141 gataaccttt tttaaagggt tggagcaaag agagcttttc gactttctgg atgcgatgag
     7201 gactgtctca aggacaggcg aaatggaaga tgacttagtc accatcttgt gggagaaaga
     7261 cctgattaac gtaagctacg tggtgattga ggattgggga gaacaaggac cgggttatca
     7321 ggaagaccta tctgttactt tagtcactca ggttatacct cagtataaga aagggagcac
     7381 cgatttcgat tttcaagaaa tctactcttc agagttagct tcacccggga cagtggttaa
     7441 tgatgaaact gaacaggcta tctccagctg ggaaaagcac accacccagg gtctagccgg
     7501 ggtaactttt cttcaaaaat ccgcagatct atccagaatt ttggcaaacc tggaccggtt
     7561 ctcagctgag gaggtaggtg agatcaacaa gatcctggaa atggatagaa cctacgtgcc
     7621 ctttgatgcg ctcctctcgg tattgtatga gactttgtat tgggaagatg aacaatcggc
     7681 attcactgag atactaaggg aagtggaggg gatgctacac acctatttaa acaaggccga
     7741 tttctcgtgc gccagtcgaa tggtaacttt gttcagagac ttagagcagg agttcaaagg
     7801 gatttgtagc tttaaagcca acagcataaa ggaggctttg gatagagcag gggacaaaga
     7861 gaggataaga tacatcatca atgccctaaa tgaccgcaag gatatggagc tttccgcagt
     7921 agaacattat ctgactttgc ttaattccaa tgtggtttct tctctgatag agatgctgag
     7981 gcatttgaga ttcttccctg cccgcaagat ggtctgcgaa gtgatggggt atatcggaaa
     8041 agatcatgtg cagatggtgg gggagggggt gtatgacccg caatggtatg tggttagaaa
     8101 catagctata gttctaggaa agataggtaa gctagagtgc gtggagtttc tcaaagccgc
     8161 tatcgtccac gcagatgcca gagttcgtcg ggaagcagta aggagtttga ccggaataga
     8221 ttcccaggaa gcaggagagg tcttgcttct ctccctaaat gacagttata acataataag
     8281 gagtatagga attagggctt tatcccaacg cggagatgcg gccgcggtca aacccttaat
     8341 gcaaattctc caggacaaaa agttcaggga gcggtcatcc gaagaaagaa aattgatgtt
     8401 ggatgctttg gctcacctag gaagagatag atccataccg gtgctggttg aacttatcaa
     8461 caaaagaagc tggtttgatc gggtaaggaa caatgaaact cgcctttttg ccctcagggc
     8521 tttaggtctg gtcaatacct tagcatctta tcaagcttta gatgagcttg gccataaagg
     8581 ggataagttg gttcgtcaaa gctgttcagc tattttaagg aaaagagaac acaaaagcta
     8641 ttaaaatata tggaaacaca aactgacaaa aaacctagga aaaacccttc aagggaaaac
     8701 cccaaagtta tagatagaat tttgaatgaa atggggaaga gcctggtaaa tcaactctac
     8761 gccactttta agactgctca aatttatgaa cgaaacaacc tggcttttgt caagcagagc
     8821 gaagctttga tcagcatcac ttatagtatt ctagagaggg agggtgaaat acacctgaga
     8881 agcaagagcg ggtatctctt cttgaacaga agcagattaa tttttaattt tgagggatat
     8941 gtcagcagcc gtttcataat ggatgaattc aaacgttgtc agatggagag catcttcata
     9001 gagaacgggg tcactaaaag agagatggat gaattcatca gccagtttgt tcacctggac
     9061 aaagataaag aggatgccct ggaggcttta gaggacaggc tcaatcagct ggaagtgcac
     9121 catgttcggg tggagagaat aaggcctgac tggataaggc caaaaactca ccaggtagct
     9181 ttctctgata tgcgaaaaga ggctaagcac actttcttta aagctatctc cgtggcacag
     9241 gagattatga ccagtgtcaa atccggcagg gcggtcaata ccgtaaaagc caaaagagtt
     9301 attcaatctt tggttgacca gatcatccaa gacgaatcca gctttttgga gttaaccgct
     9361 attaaaaatt acgatgaata tacttttgcc cattcagtca atgtctgcgt tttagccatt
     9421 gcctttggtt tgaaattggg attggaccga aagaaactta gcgaattggg tttctccgct
     9481 ctgttccatg atat
//
LOCUS       DASWLN010000130         4503 bp    DNA     linear   ENV 20-FEB-2024
DEFINITION  MAG TPA_asm: Candidatus Zixiibacteriota bacterium isolate
            SMAG_U2949 k141_997357, whole genome shotgun sequence.
ACCESSION   DASWLN010000130 DASWLN010000000
VERSION     DASWLN010000130.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37960594
            Sequence Read Archive: SRR10376091
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Candidatus Zixiibacteriota bacterium (soil metagenome)
  ORGANISM  Candidatus Zixiibacteriota bacterium
            Bacteria.
REFERENCE   1  (bases 1 to 4503)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 4503)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (26-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/12/2024 08:28:26
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 1,440
            CDSs (total)                      :: 1,416
            Genes (coding)                    :: 1,412
            CDSs (with protein)               :: 1,412
            Genes (RNA)                       :: 24
            rRNAs                             :: 1 (16S)
            partial rRNAs                     :: 1 (16S)
            tRNAs                             :: 21
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 4
            CDSs (without protein)            :: 4
            Pseudo Genes (ambiguous residues) :: 0 of 4
            Pseudo Genes (frameshifted)       :: 0 of 4
            Pseudo Genes (incomplete)         :: 3 of 4
            Pseudo Genes (internal stop)      :: 1 of 4
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..4503
                     /organism="Candidatus Zixiibacteriota bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_997357"
                     /isolate="SMAG_U2949"
                     /isolation_source="soil"
                     /db_xref="taxon:2053527"
                     /environmental_sample
                     /country="USA: Oregon"
                     /lat_lon="42.99 N 123.63 W"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            complement(<1..770)
                     /locus_tag="VGB16_04305"
     CDS             complement(<1..770)
                     /locus_tag="VGB16_04305"
                     /inference="COORDINATES: protein motif:HMM:NF015983.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003824 - catalytic activity [Evidence
                     IEA];
                     GO_function: GO:0051539 - 4 iron, 4 sulfur cluster binding
                     [Evidence IEA];
                     GO_function: GO:1904047 - S-adenosyl-L-methionine binding
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="radical SAM protein"
                     /protein_id="HEX9916939.1"
                     /translation="MTSSKKIGILFKYAWSKFKRRPLFLNLEVTKRCNARCKLCDYWK
                     GPHEAELKDYAPVVQKINPLIISFTGGEPLVRKDIFNLIQSIKENGRLFYLTMVTNGA
                     LLNLEKAERLKKAGIDQLSVSLDFLDERHDQTRGIKGLFAHLSNLIPLLSHIGFDNVA
                     LNTVIMENNLDQITQIAEQAWGWGVKVSYSSYCEMKNNDRSLSVKSENLDKLKRIIAK
                     LIQYKSSLGNITNSTYYLERIPIYFETGKIDGCLAGQR"
     gene            complement(857..2269)
                     /locus_tag="VGB16_04310"
     CDS             complement(857..2269)
                     /locus_tag="VGB16_04310"
                     /inference="COORDINATES: protein motif:HMM:NF013799.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="alkaline phosphatase family protein"
                     /protein_id="HEX9916940.1"
                     /translation="MRQFKHCIIFLIDGARPDVMEKMMKRGNLPNIQRYLLEPGSYTK
                     AVTSFPSTTGPAYIPILTGCYPGTADVPGIRWFDKEESSRKRLSLRRQRSYMGVEAPL
                     FNSDLRKDKLTLFEIFQNSINIFNPLTRGINSTNNLTRFSKTLYYLFVHLTSTWQIVD
                     WATHRYLFRAIRKKPEFIFALFPAVDCLSHFTHPFSKTVLDAYLEVDKFIGKTVQHLK
                     RRGMLDQTLLILTSDHGLTSTHTHLDLVDFLDKRGYRALHHPRITRRQTKAAVMVSGN
                     SMAHVYLKNGQGWGKRIYFEELEKNHARLLSDLINLHEVALVACADEDGGVCVLRNGG
                     KARIREREGNIDYSVEGEDPFGYKGLPQRLNPTTSLEHTFDSDYPDALVQLIQIFKSR
                     RSGDIILSAQKGFDLRARFEWPEHKASHGSLHREHMLVPLFINTKIKTRKIRTVDIFP
                     SILELLGRENNNEIDGKSFV"
     gene            complement(2590..3822)
                     /locus_tag="VGB16_04315"
     CDS             complement(2590..3822)
                     /locus_tag="VGB16_04315"
                     /inference="COORDINATES: protein motif:HMM:NF013753.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="transposase"
                     /protein_id="HEX9916941.1"
                     /translation="MGKRDVKIKIVKPRIPKRIICPTCGMKQKFRKKKEHWKMVRDMD
                     VNVTVSLKVRMVYAKCLNPDCAVGSFALPIPGIEKRARATNRLKSEAVASLIDDNSTT
                     PRTARRLNRSFNTTGSKSSIDRWKHQEADKYQIKEIIQKLSFSGVLCVDEYKPSRAKF
                     YDLIDSDGQTGKILYVTPIEYLGRGLIKNHFEKLKSLGITPQVIVFDMRKVFPNAARK
                     AFPGVLIQHDYFHVMKTIHWHLRNAMAEFREKLKEKGFYTQDLWDCRWIILKNLENWT
                     SEDHRRMQNLLARYSGTVIEDILVIKEQIRDIFLKSQDKKEAYLKRDELCSNPILKQN
                     QHFDDIFKFLSTPYFEHMVTFLDHPHVPRSGNSENVIRVWRQMEKVRYGFKTAKGRLD
                     HLKLYQISRYLKGNFFKT"
     gene            4050..>4503
                     /locus_tag="VGB16_04320"
     CDS             4050..>4503
                     /locus_tag="VGB16_04320"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEX9916942.1"
                     /translation="MRYLSIIFCAVLVLFSLRQAQAEVPKLINYQGKLTTPKDTCEIR
                     VVSVNDTISPQSPCGFNVRQDILNKVIIDVVGTDSTDSFTVDVRALVDDGAHRIDYRN
                     NMTMPCTIMVSVDNWSPGTQNLFVHVENQETGQECVPSPLIIPLCVLLI"
BASE COUNT         1208 a         1003 c          903 g         1389 t
ORIGIN      
        1 caacgctggc ctgccaaaca cccatctatc ttcccggttt cgaaatagat gggaattctt
       61 tccagataat aagtggagtt ggttatattc cccaatgaag atttgtattg aatcagtttg
      121 gcaattatcc ttttgagctt atctaaattt tcagacttaa cagataagga acgatcatta
      181 tttttcatct cgcaataaga actgtaggac actttgaccc cccaacccca ggcctgctct
      241 gcgatctggg taatctgatc caggttattc tccattatca cggtattgag ggcaacgtta
      301 tcaaaaccaa tatgggaaag aagaggtatg agattcgaga ggtgggcaaa aagaccctta
      361 attcccctgg tttggtcatg cctttcatcc aaaaaatcca gagagacaga caattgatcg
      421 attccggctt ttttcagcct ttcagctttt tctaagttca aaagagctcc attggtaacc
      481 atggtcaaat aaaagagtct cccattttct tttatgctct ggattagatt aaaaatatct
      541 ttgcgcacca aaggctcacc gccggtgaag gaaattatta agggatttat cttttgaacc
      601 acaggggcat agtctttcag ttccgcttca tgcggaccct tccagtaatc gcaaagcttg
      661 catctggcat tgcaccgctt ggttacttca aggtttaaaa agagcggtct tcttttgaat
      721 ttgctccaag catacttgaa aagtatacct attttcttgg aagaggtcat ctttttctca
      781 tccgattaag aaataacgga tgacgaataa cgaatgacga ataaaagcat tcgtcataaa
      841 tcattcgtca tttttactat acaaagcttt ttccgtctat ttcattgttg ttttcccgtc
      901 ccaagagttc cagaatggag gggaagatgt caactgttct gatttttctt gttttgattt
      961 tggtatttat gaataaggga accaacatat gctcccggtg aagtgaacca tgagaggctt
     1021 tatgttcggg ccactcaaat cgagccctga gatcgaatcc cttttgagca gaaagaatta
     1081 tatcgccgga tcttctggat ttgaaaatct gaataagctg gactaaagca tcgggataat
     1141 cggagtcaaa ggtgtgctct aagctggttg tgggattaag tctttgggga agccctttat
     1201 aaccaaatgg atcttctcct tccacggaat aatcgatgtt tccctcccgc tcccggattc
     1261 tggctttccc tccattacgc aagacacaaa cacctccatc ttcatccgcg caggcgacca
     1321 aagccacttc atgcaaatta atcaaatctg aaagaagtct ggcatgattt ttttccagct
     1381 cctcgaaata gattctcttt ccccaacctt gcccgttttt caaataaaca tgagccatgg
     1441 aatttccaga gaccatcacc gccgccttgg tttgtctgcg ggtgattcgg ggatggtgta
     1501 aggctcggta gcctctttta tcaaggaaat cgaccaaatc caaatgcgtg tgagtggagg
     1561 tcaatccatg atcactggta aggatcaaca aggtttgatc aagcattcct cttcttttta
     1621 aatgctggac agtttttcct ataaacttat ccacctccag gtaggcgtcc aaaaccgttt
     1681 tggagaaagg gtgagtgaaa tgagagagac aatcaaccgc ggggaacagg gcaaaaataa
     1741 actcaggttt ctttcttatg gctctaaaca gatacctatg ggtggcccag tccacgatct
     1801 gccaggtcga ggtaaggtgg acgaagaggt aatacaaagt cttggagaat ctggtcaggt
     1861 tatttgtgga gtttatcccc cgggttaagg ggttaaagat gttgatggag ttttggaaaa
     1921 tctcaaaaag ggtaagttta tctttcctca ggtcggaatt gaataaggga gcttccaccc
     1981 ccatgtagct tctttgtctt ctaagactta atctctttcg actgctctcc tccttgtcaa
     2041 accatctgat cccgggcaca tcggcggttc cgggataaca tccggtcagg atgggaatgt
     2101 atgccggtcc cgtggtggag gggaaggacg taacggcttt agtatagcta cctggttcca
     2161 ataaatatct ctggatgttg ggtaaattcc cccttttcat catcttctcc atcacgtccg
     2221 gccgagcccc atcgatcaga aatattatgc agtgcttgaa ttgtctcatc gagtcctcag
     2281 aattcctgat aagcaacaga aatcacaccc acagtttatc tgtccggagc aatggaggat
     2341 atttttggaa agacatccgg aagttaagct ccatactttt tctatggatt ctgtagacct
     2401 gtaaacatcc atttcattca ctaaaagctt tttctcctcc caaatattaa ctcggagact
     2461 cttatcaaaa ttatggataa atttacggat ttgcttggca gtttgtcaag caaaaaatgg
     2521 gaagctttcc cacatttgtg gcaaatattt ttggtaacgt attttgtggt attaggttaa
     2581 atgaaatttt catgttttga agaagtttcc tttgagatat cggctgattt ggtatagttt
     2641 tagatgatct aatcttccct tagctgtttt gaatccatac ctaactttct ccatctgtct
     2701 ccacactcgg atcacattct cggagttacc gcttcgggga acatggggat gatctaagaa
     2761 ggtaaccata tgctcaaagt agggggtaga aagaaatttg aaaatatcat caaaatgttg
     2821 gttctgcttg aggatagggt ttgagcagag ttcatctctt tttaagtatg cttctttttt
     2881 gtcttgactc tttaagaaga tgtctcgaat ctgttccttg ataaccaaaa tgtcttctat
     2941 cactgttccg gaatagcgag ctaaaaggtt ctgcatcctt ctatggtcct cactggtcca
     3001 gttttctaga tttttaagga ttatccatcg acagtcccac aggtcttgcg tgtagaaccc
     3061 tttttctttt agtttttccc tgaattcagc catggcattc ctcaaatgcc agtgaatagt
     3121 cttcatgaca tgaaagtaat cgtgttggat caagactcct gggaaagctt ttctggctgc
     3181 attagggaag acttttctca tatcaaagac gatcacttga ggagttatcc ccagactctt
     3241 aagtttttca aagtgatttt tgatgagacc acgtcccaga tactcaatgg gtgtgacata
     3301 caatatcttt ccggtctgtc catcgctatc aatcaggtca tagaacttgg ctctgctggg
     3361 tttatattca tctacacaga ggactccaga aaaagaaagc ttctggataa tctctttgat
     3421 ctgatattta tccgcttctt gatgtttcca ccggtcaatg ctggatttac ttccggtggt
     3481 gttaaaagag cggttcagcc ttctggccgt ccttggggta gtggaattat catcaataag
     3541 gcttgccacc gcctctgatt ttaacctgtt tgtagctcta gccctctttt ctatcccggg
     3601 tatcggcaga gcaaaacttc ccacagcaca atctggattt aaacacttcg catataccat
     3661 ccggaccttc aatgataccg taacattcac atccatatcc ctaaccatct tccagtgctc
     3721 ttttttcttc ctgaattttt gtttcatccc acaagttgga cagattattc ttttgggaat
     3781 ccttggcttc actattttta tcttaacatc tctcttcccc atagttaggt tcctcctatt
     3841 tttggagaaa cctaactcaa cccttcccta aaaccttcct catcccccct caaaattcaa
     3901 aaacttgcca caaaaacggg aaagtctcgc aaatttccct tgacaagtca aaaagtttga
     3961 ttatgattta gatagaaatc cttttttgta tgaaagatat ataaaatgag atgcccttct
     4021 tttctctaac cccaaacccc ggaggtctta tgcgatacct atctatcatt ttttgtgccg
     4081 tgcttgttct cttctctctc cgccaggcac aagcagaagt ccccaaactc atcaactatc
     4141 aaggcaaatt gacaacaccg aaagacacct gtgagattcg agtggtaagc gttaacgaca
     4201 ctatctcccc gcaatctccc tgtggattca acgtaaggca agatatacta aataaggtga
     4261 taatcgatgt ggtgggaacc gactcgactg actcgttcac tgtcgatgtg cgagccttgg
     4321 tggatgatgg agctcatcgt atcgattata gaaacaacat gaccatgccc tgcaccataa
     4381 tggtctctgt ggacaattgg tccccaggaa cgcaaaatct ttttgtgcat gtggaaaacc
     4441 aggaaaccgg acaagagtgt gtcccctctc cactaataat tcctctgtgt gttctactga
     4501 ttt
//
LOCUS       DASWLN010000131        12588 bp    DNA     linear   ENV 20-FEB-2024
DEFINITION  MAG TPA_asm: Candidatus Zixiibacteriota bacterium isolate
            SMAG_U2949 k141_100520, whole genome shotgun sequence.
ACCESSION   DASWLN010000131 DASWLN010000000
VERSION     DASWLN010000131.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37960594
            Sequence Read Archive: SRR10376091
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Candidatus Zixiibacteriota bacterium (soil metagenome)
  ORGANISM  Candidatus Zixiibacteriota bacterium
            Bacteria.
REFERENCE   1  (bases 1 to 12588)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 12588)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (26-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/12/2024 08:28:26
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 1,440
            CDSs (total)                      :: 1,416
            Genes (coding)                    :: 1,412
            CDSs (with protein)               :: 1,412
            Genes (RNA)                       :: 24
            rRNAs                             :: 1 (16S)
            partial rRNAs                     :: 1 (16S)
            tRNAs                             :: 21
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 4
            CDSs (without protein)            :: 4
            Pseudo Genes (ambiguous residues) :: 0 of 4
            Pseudo Genes (frameshifted)       :: 0 of 4
            Pseudo Genes (incomplete)         :: 3 of 4
            Pseudo Genes (internal stop)      :: 1 of 4
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..12588
                     /organism="Candidatus Zixiibacteriota bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_100520"
                     /isolate="SMAG_U2949"
                     /isolation_source="soil"
                     /db_xref="taxon:2053527"
                     /environmental_sample
                     /country="USA: Oregon"
                     /lat_lon="42.99 N 123.63 W"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            225..2111
                     /gene="pepF"
                     /locus_tag="VGB16_04325"
     CDS             225..2111
                     /gene="pepF"
                     /locus_tag="VGB16_04325"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_019122393.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004222 - metalloendopeptidase activity
                     [Evidence IEA];
                     GO_process: GO:0006508 - proteolysis [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="oligoendopeptidase F"
                     /protein_id="HEX9916943.1"
                     /translation="MKRQNRAIWVLLAGIIMVLSISAVFAQTPAKGIPQRSDIPGKYK
                     WKLEDIYATSQLWEADYARLESSLSRFATFKGKLGKSPKILLNCLQLQDSLGIIMEQL
                     WVYANMRLDEDTRISFHQAMADRVSNLSTQFSQAVSFIEPDILALSKGKLEGFVKKNR
                     ELLVYQHYFDNLMRTKAHILPPEQEELLALAGKFAGTPRKTFSMLDYADIKYPSIKDE
                     NGAEVELTKERYSKFLESTDRRVRKDASNAYNSAYLNYINTLAANLSAELSKDIFFAK
                     ARKYNSSLEVTLDGSNIPASVYDNLVQTVDANLAPLHRYISLRKKVLELDTLYTFDLW
                     FPLVPKAKMDIPYDQAVGTIIEALSPLGEKYVNDLKMGFSSGWVDVYETQGKRSGAYS
                     LGVYTAHPYVLVNYNNTLENMFTIAHEMGHSLNAYYTYKRQPYIYSDSPTFVAEVASA
                     CNEAIMIDYLIKHTQDKDKKAYLLEYYIQQIIGTFYGQVFYSEFEKEIHDQMESGQGL
                     TAESMRKTYRDIYQKFYGPEVVIGPINDLGWARIEHFYKNFYVFQYSTGYAASIALSR
                     KILEGKKESVDGYLTFLEQGNSDYPIELLKRAGVDMTSPGPIQETVKLFDDLVSQLEK
                     LLLE"
     gene            2442..3854
                     /locus_tag="VGB16_04330"
     CDS             2442..3854
                     /locus_tag="VGB16_04330"
                     /inference="COORDINATES: protein motif:HMM:NF013961.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="transglutaminase domain-containing protein"
                     /protein_id="HEX9916944.1"
                     /translation="MMKKYIVCVLVSLIFCGVVFAQEKNPATQAEKLYEEANNAYTNK
                     EYAKAIPLYLEAMACPEASEWRATILYNVSCCYSLLGIADSAFEYLDQSIRAGNTDYR
                     WIKKDTDFSSLGKNHPKEFEEMILRARAARKNEILTKSPIAVVECSNYNGSTEITEYF
                     NDSLDSPEMDSLRNRYQLRRVVESGKTEFEKMKLLLDWVANRWKHNGDNMAIDRSALA
                     ILKAAEKGERFCCANYADVLVGCMKALGYPAHFVGLRRDDAAYYMGGGHGCIEVWSNQ
                     YQKWILLDGQNNAWWESGGLPLSAYECHQLLVSGKEDEMKFVGQHKQVDYDHIKPDWS
                     VHFYRVVCYWRGVGFELVSDTIAPDLFLQGSPVDYEITDDYTTVYPRLNQTKITLKHT
                     NLKAPSDTLDVVLTHTMPCFDKFMVRINGSDWKESKDTFRWVLDKGDNIIEAKAVNLA
                     GIEGRPSSIVLRNNIETSGE"
     gene            4103..4723
                     /locus_tag="VGB16_04335"
     CDS             4103..4723
                     /locus_tag="VGB16_04335"
                     /inference="COORDINATES: protein motif:HMM:NF013982.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF47 domain-containing protein"
                     /protein_id="HEX9916945.1"
                     /translation="MKLPRFIPREEKFFDLFKDSAKNVLVGAEALRDLVNNYQDIETK
                     VRRIKDIEHRGDAITHEIINKLNKTFVTPLDREDIHQIASKLDDVLDAIEGISSRLLI
                     FKIKQLTPACIKLVDIVYKGAVQIEKVISDLEHFDNLHPFCIEINRLENDADAISQNM
                     IAQVLDEESDWRLAIKWKEIYGRLETAADHCEDIANAIESIVVKNA"
     gene            4716..5720
                     /locus_tag="VGB16_04340"
     CDS             4716..5720
                     /locus_tag="VGB16_04340"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_012414226.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0016020 - membrane [Evidence IEA];
                     GO_process: GO:0006817 - phosphate ion transport [Evidence
                     IEA];
                     GO_process: GO:0055085 - transmembrane transport [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="inorganic phosphate transporter"
                     /protein_id="HEX9916946.1"
                     /translation="MLSLIFIIFIILIALAFDFTNGLHDAANSIATVVSTRVLSPRQA
                     VVWAAFFNFVAFLIFGTAVATTIGKGLINISVVTPWVIFAALIGAISWNIFTWYLGLP
                     TSSSHALIGGYAGAAIVKAGFGVIIASGWIKTLVFMVLAPVIGLVLGLVFIIITTWIV
                     HKKHPTLINKWSRRFQLFSAALYSLGHGGNDAQKTMGIITSLLVSAGLLKSFHVPLWV
                     VLSAHAAIAFGTLSGGWRIVKTMGQKITKLRPIDGFSAETASAISIFLATHLGVPVST
                     THVITGAISGVGGVKRLSAVRWGITLKIVWAWLLTIPGAALTAGVFYFLLETGIKIFS
                     "
     gene            complement(6197..6916)
                     /locus_tag="VGB16_04345"
     CDS             complement(6197..6916)
                     /locus_tag="VGB16_04345"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEX9916947.1"
                     /translation="MRSRKFLVSLCLLSLLVFVGCAYTTERTHPNFSERRKEIKSIGI
                     MPPEFHMFERTATIDEPKPELDAEADSNIVNALNEVLKEGGFVSVAVPMNDSALVYDQ
                     ELALDLTRQKQSFDKACDSAIVSKKKTVNLTVDPNVGVFADLAQTDYLLFVRGMGYQT
                     SGGAKAKDVAIGALSAVLFGTYAGSQWTGMVLEIGLVDANSAELLWYNRNTVDQSNYN
                     ARQPKSAKKLCKKILEKLVKK"
     gene            complement(6919..8178)
                     /locus_tag="VGB16_04350"
     CDS             complement(6919..8178)
                     /locus_tag="VGB16_04350"
                     /EC_number="3.4.24.-"
                     /inference="COORDINATES: protein motif:HMM:NF013594.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004222 - metalloendopeptidase activity
                     [Evidence IEA];
                     GO_process: GO:0006508 - proteolysis [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="M48 family metalloprotease"
                     /protein_id="HEX9916948.1"
                     /translation="MIYRRSYLLLLLLATLLIIGCASAGKEIVVSDLNSVSQLPKGDK
                     KLWDMSRYEENTLTKSGLIYTDPAVSKYLQIIGERMIPPEYKNSIFKFRFYVILDPEL
                     NAFALPNGAIYLHVGLLSKLEKPDQLAFILGHEMTHSLRRHASRQYAHIQNVSGFAQF
                     LSVATAVGFSQSGSNMTGFWNSLAQNGLALTAMAAVSGYSRSDEKEADLNALDLMEKA
                     GYDKIQAVRSLEILLEEYPDKSTTANFFYGNHPLTKQRLGYIEETIGYKGEELKPEID
                     STYEQAVSRLKLKNVELFVISSKYDKALKQASRIEVNYSGDPTFHYWCGEAYRLSGSK
                     PDTLQLAQDQYQKALDLDSSFALAHLGLAQVWEARKDKNEALTHYETYLIMDPQTKKK
                     RYIESKIKSLKQEPIPQTPETNKKQEG"
     gene            complement(8204..10378)
                     /locus_tag="VGB16_04355"
     CDS             complement(8204..10378)
                     /locus_tag="VGB16_04355"
                     /inference="COORDINATES: protein motif:HMM:NF021942.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="S46 family peptidase"
                     /protein_id="HEX9916949.1"
                     /translation="MIKRIFLGLLVLFILGLFFAICLADEGMWMLDSIDKLPWSKMRE
                     MGLKLSPKDVYNPKGDGIARAIVNVAGASGSFVSPEGLIVTNHHVAFSAIQRQSKPGQ
                     NYLKEGFHAKTKAEEIPAIGYNVYVTLSMDEITDKVLVAVDDRMDDLERYKAIEKRTK
                     QIIDEAEAAKDVKCEVVSVYGGMQYYLFTYFKIKDVRIVYVPPLSIGEYGGEIDNWMW
                     PRHTGDFSFLRAYVAGDGKSADYSPDNVPYRPEIYLKVSSAGIKEGDFAMMIGFPGWT
                     ARYSSSFSVEDMQNYYYFVDIGIREDLIKIMEQSSQEDSSVALRLSSKIKGIYNYLKK
                     NQGVLEGFEKSKVLEHKREEERNLAQYLKENPQLDQKYGDVLPSLEKLYEDHKTYRDK
                     DFILSWMISYCDFLGLASTINKWSIEKQKQDMEREPEYMDREIPQTRKNLENAQINLV
                     PSCDKKILAYFLKKAHELPPGQKIEVVEKLFENKKGSPQDQVISDFVSYLYANTKIGT
                     LQERMKMFDMTQEELEKLVDPFIDFARELEKEREVLKEKDKRFNGALSKLEPKLIQAY
                     AEWKKGLTYPDANGTIRFNYGTVQGYIPGDAVFYKWITILTGVMQKNTGKEPFEVPIQ
                     LEEIYQKKDFGKYFDKSIGDVPVNFLTTNDGTNGNSGSPIMNGKGELIGLTFDGNYES
                     MAADYEFEEEINRSINVDIRYVLFILDKVYHIENVLGELVIH"
     gene            complement(10580..11515)
                     /locus_tag="VGB16_04360"
     CDS             complement(10580..11515)
                     /locus_tag="VGB16_04360"
                     /inference="COORDINATES: protein motif:HMM:NF033709.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="PorV/PorQ family protein"
                     /protein_id="HEX9916950.1"
                     /translation="MRSKLNLLIVLAVLVLLAINASDGFAIGGNVGTRSAQFLKIGAG
                     ARAVGMGSAFVGLANDATAIYWNPAGLAYIDKTQFSFMHLWWFQDIAYEYFAHAQPTK
                     YGTIGWSLAYLHMDPLQGRDVEGEPTSDFRASDMAITLGYGNRIAKDIFIGGSIKYIN
                     EKIEDEMASTFAFDLGFLHRTQFKNLFLGGSFQNWGGTLKFAEEPDKLPSNAKLGLCY
                     TVSLARNPLNLAVDFVIPSDAKTGLHLGAEYIYNHMIMGRLGYRTETDLGFLSGISFG
                     VGFLYARTESYRIDYAFVPQGVLGNTHTISLTVTF"
     gene            complement(11523..12260)
                     /locus_tag="VGB16_04365"
     CDS             complement(11523..12260)
                     /locus_tag="VGB16_04365"
                     /inference="COORDINATES: protein motif:HMM:TIGR04183.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0005576 - extracellular region [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="T9SS type A sorting domain-containing protein"
                     /protein_id="HEX9916951.1"
                     /translation="MRFRIITLIIISLVCLIFPSLAFSQEDPDKKEIIITVSFAFNFI
                     DSIPPYLDSVAPPRNAKRVPPDTNIVFHVKDSGYGVDLSSIRLWIGGQEIIGFSSSIE
                     GSPSDYKVTYFPPTQFPWGGEILVRVSAHDIASPPNQLDTSYSFKIEPGPALTLANAS
                     IFPNPFKPYQGHTFVTFDGLTTQAKIEVFTIVGERVYTVDETDGDGKVVWNVVNSDGK
                     KLASGVYICRITNDKGEEKFFKLAVIR"
BASE COUNT         3519 a         2720 c         2774 g         3575 t
ORIGIN      
        1 gggtcagggg agattctaga gcggcacatc tttggagcga ttccacaagt cagatataag
       61 tgcaatcaca ctggtaatag agtataagtc aagaagggga ttggataccc caaaaatccc
      121 ttgcctgact ttagcaattc cactataatg gcagaagccc aaataaacaa aaacgcaatc
      181 agtttaacat catcaactca aaccctcaaa atatggagtt caaagtgaaa aggcaaaatc
      241 gagccatttg ggtattgtta gcaggaatta ttatggttct gtctatctca gctgtttttg
      301 ctcaaacccc tgccaaggga attcctcaaa gaagcgatat tccgggaaaa tacaaatgga
      361 aactggaaga tatttatgcc accagccaat tatgggaagc cgattatgca agattggaat
      421 cctcgctttc acggttcgct acttttaaag gtaagttagg taaatcgcct aagattctgt
      481 tgaactgcct gcaattgcag gatagtttgg gaatcattat ggaacaactc tgggtttatg
      541 ctaatatgcg tttggatgaa gacactcgaa tttcgttcca tcaggcaatg gctgaccgcg
      601 tttcgaactt aagcacccag ttcagtcaag ccgtgtcttt tattgagccg gacattttgg
      661 ctctttctaa aggaaagctt gaaggctttg ttaagaaaaa cagagaactt ttagtttatc
      721 agcattattt tgataaccta atgagaacca aagcccacat tttgcctccg gaacaggaag
      781 agcttttggc tttggctggc aagtttgccg gaactccccg aaagacgttt tctatgttag
      841 attatgcgga tataaaatat ccctctataa aagacgagaa tggagcggaa gtcgagctta
      901 ctaaggaacg atattcgaag ttcctcgaat caacagacag gagagtaaga aaagatgcaa
      961 gcaacgccta caactctgcc tatttaaact acatcaatac tctggcggcg aatttaagcg
     1021 ccgagctttc caaggatatt ttctttgcta aagccagaaa atacaattcc tctttggagg
     1081 tgacgctgga tggatctaat attcccgctt cggtttatga taatttggtc caaaccgtag
     1141 atgccaactt ggctccgctt caccgttata tttccttgcg caaaaaagtc ttagagctgg
     1201 atactttata tacctttgac ctctggttcc ctttggtccc caaagccaaa atggatattc
     1261 cctacgacca ggcagttgga accataatag aagcattgtc tcctttggga gaaaagtacg
     1321 ttaatgattt gaaaatggga tttagttccg gctgggtaga tgtttatgag acccagggaa
     1381 agagaagcgg ggcctactcc ttgggtgttt atactgccca tccttatgtg ctagtcaatt
     1441 acaacaacac cctggagaat atgttcacca tagctcatga aatgggtcac tctctaaacg
     1501 catattatac ttacaaaaga caaccctaca tttattctga cagcccgact tttgtggcgg
     1561 aagttgcctc cgcctgcaat gaagccatta tgattgatta tttgataaaa cacactcaag
     1621 ataaagacaa gaaagcttat cttttggaat attacattca gcaaatcata gggactttct
     1681 acggtcaggt gttttactcc gagtttgaaa aagagattca cgaccagatg gaaagcggac
     1741 aaggactgac cgccgagtca atgagaaaga cctaccggga tatttaccaa aagttctatg
     1801 gtccggaagt ggtgataggt ccaatcaacg atttgggctg ggcaaggatt gagcattttt
     1861 ataaaaactt ctatgttttt caatattcca ccggctatgc tgcttccata gctttatccc
     1921 gaaagatcct agaaggtaaa aaagaaagcg ttgatggcta cttaaccttc ttagagcaag
     1981 ggaattcaga ttatccgatt gagcttttga aaagagctgg agtggatatg acctctcctg
     2041 gaccgatcca ggaaactgtt aagcttttcg atgacttagt ttcacaatta gaaaaactat
     2101 tgctggaata gcagtaagtc aggaactcta aacttcttag cagagcaggc ctgacaataa
     2161 gacaacaatt tcagatcctc ttcattaacc cctcttaatc tccccttatc aggggagaaa
     2221 tgaccggtgt ccccctgaca agggggacaa tagggggttg gttggtagga ggtcagaagc
     2281 acagggcttc tgacctccat tgctattgct ggagtagatt ctccggacag actgtcgcaa
     2341 ttttctgcaa gagaatgaaa atctcgaaac agacagaagt gtaattccgt atactcaaac
     2401 tgagatgctg tagaaatcat aaaaatcaag gaaggtggct tatgatgaaa aagtatatcg
     2461 tgtgtgtgct cgtttctctt atcttttgtg gagttgtgtt tgcgcaggag aaaaatccgg
     2521 caacccaagc agaaaagcta tacgaagaag ccaataatgc ctacaccaac aaagaatacg
     2581 caaaggcaat tccgctctat ttggaggcta tggcttgtcc agaagcatca gagtggcgag
     2641 caactattct ctataatgta agctgttgtt attccttatt aggcattgcc gacagtgcat
     2701 tcgaatatct ggaccagagc ataagggctg gtaatacaga ttatcgttgg atcaaaaaag
     2761 atacggattt cagctctctg gggaaaaatc atccaaaaga attcgaggag atgatccttc
     2821 gggcgcgagc cgcaagaaag aatgagatac tgacaaaaag tccgatagca gtggtggagt
     2881 gcagtaatta taatgggtct acagagatta cagaatactt caatgacagt ctggatagtc
     2941 ctgaaatgga ttctcttcga aacagatatc agcttcgcag ggttgttgag agcggaaaga
     3001 ccgaatttga aaaaatgaag cttttgcttg actgggtagc taataggtgg aagcataatg
     3061 gggataatat ggcaatagat cggagtgctt tggctatcct taaagcggca gaaaaaggag
     3121 agcgattctg ctgtgccaat tacgccgatg ttcttgtcgg ctgcatgaaa gcccttggat
     3181 accctgcgca ttttgtagga ttaagaagag acgacgcggc ctactatatg ggtggggggc
     3241 acgggtgtat agaagtgtgg agcaaccagt accaaaaatg gatcctgctt gacggtcaga
     3301 ataacgcctg gtgggagtct ggcggtctgc cattgagcgc ttatgaatgc caccagctac
     3361 ttgtgagcgg taaagaggac gagatgaagt tcgtcggaca gcacaaacag gtcgactacg
     3421 atcacattaa accggattgg agtgtccact tttatcgggt agtatgttac tggcggggag
     3481 ttggtttcga acttgtctca gacaccattg caccagattt attcttacag ggaagtcccg
     3541 ttgattacga aataacggat gattacacaa ctgtctatcc ccgactgaac caaaccaaga
     3601 tcaccttgaa gcacactaat ctgaaagctc catcggacac tttggatgtt gtgcttaccc
     3661 acactatgcc ctgcttcgat aagttcatgg ttcgcattaa cggatcggac tggaaagagt
     3721 caaaagacac atttagatgg gtcttggaca aaggagataa tataattgaa gccaaagccg
     3781 tgaatttggc gggcatcgaa ggcaggccga gcagcattgt attgagaaac aacattgaaa
     3841 cttcagggga gtgacgccca atctttagag agaaaatagt agtagcttaa ggagcccagt
     3901 gctcctgaga aggtgctttg catctctcca gtcgttcaat caagggaggt gcaataacat
     3961 tcccttcaac accttatctt cttttattct tcatctaaat cttttccttg ccaaatttta
     4021 aatagcgatt atatttgggc aaagtctgat ttatcccagc gcaaaagtat gtaaagaatc
     4081 ataaaacgga ggaaatacgg caatgaagtt accgcgattc atacccagag aagaaaagtt
     4141 ttttgacctt tttaaggatt cggccaaaaa tgtgttagta ggggcagagg cattaaggga
     4201 tttagttaat aattatcagg atattgaaac caaggtgaga aggattaaag atatagagca
     4261 tcgcggggat gcgattaccc atgaaatcat aaataagctg aacaaaacct tcgtcacccc
     4321 tctggatagg gaagacatac atcagatagc ttcaaagttg gatgatgtct tagatgccat
     4381 agaggggatt tccagtcggc ttttgatttt caagattaaa caactaaccc cagcttgcat
     4441 taagttagtg gacatcgtct ataagggggc agtgcagata gaaaaggtaa tttcggattt
     4501 agagcatttc gacaacttac atcccttctg catagagatt aaccgtttgg agaacgatgc
     4561 ggatgcgatt tcccagaata tgatagctca ggttctggat gaggagtcag actggagatt
     4621 ggctatcaaa tggaaagaaa tttatggtcg cctggagact gcggctgacc attgtgaaga
     4681 tattgccaac gccatagaaa gcatagtcgt aaaaaatgct tagtttaatc ttcattatct
     4741 tcattattct gattgcctta gcttttgatt tcaccaatgg tctgcacgat gcggctaatt
     4801 ccatcgccac ggttgtttct acccgtgttt tatccccccg ccaagctgtg gtctgggcgg
     4861 cttttttcaa tttcgtggct tttttgattt ttggcacggc tgtggccacc acgattggca
     4921 aaggcttgat taacattagc gtggtaactc cgtgggtgat atttgctgct ctgattggcg
     4981 ctatcagctg gaatattttt acctggtatt taggcttgcc caccagctct tcgcacgctt
     5041 taattggcgg ctatgctgga gcggcgattg tcaaggctgg ctttggcgta attattgcca
     5101 gtggctggat taagactctg gtttttatgg ttttggcacc ggtaattggt ttggtcttag
     5161 gattggtttt catcatcatt acaacctgga tagtgcacaa gaaacacccc acccttatta
     5221 acaaatggtc ccgccgtttc caactttttt ccgcggcttt gtatagtctg ggccatggtg
     5281 gcaatgatgc acagaaaact atgggtatta ttaccagttt attggtttcc gctggtttgc
     5341 tcaaaagctt tcatgtacct ctttgggtcg tgctgagtgc tcacgctgcc attgcttttg
     5401 gtactctgtc tggcggctgg cgtattgtga agaccatggg tcagaaaatt accaagttga
     5461 gaccgattga tggttttagt gcggaaaccg ccagcgccat cagtattttc ttagccaccc
     5521 atttaggtgt accggtcagc accacacatg tcattaccgg cgctatctca ggcgtgggtg
     5581 gggtcaaacg gctctctgcg gtacgctggg gtattactct gaaaattgtc tgggcatggc
     5641 ttctgactat ccccggtgcc gctttgacag ctggtgtttt ctactttttg cttgaaacag
     5701 ggattaagat attttcgtaa gaaattaaat taagaaagcc ctcatccatt ttaatggacg
     5761 agggcttttt atttgagaac aaattccatt ctaaataaaa aaatcggatc tttcttaaat
     5821 attggggagt attttgtgta acctattgac aaaaggatag attttgtcat gctgaaccct
     5881 gaaagtgtca tgctgaactt ggttcagcat ctcttcaggg catgcttggt tcagcatctc
     5941 ttgagaccct gaaatcgcag accctgaatt tgtttcaggg catggttcag ggtgacaatc
     6001 accaagggag gggtgaagtc cgaagagtgc gggtggagat gcggttcgac cttgctcacc
     6061 gtcctgagca aagtcgaagg aaactccacc cctaggcgag tacaacatgt attaccccgt
     6121 ggaggacaac taaagaaagc cgtagccaaa cactatttcc acattaaata cgaaagaacg
     6181 aaaaaatcta agatgcttac ttcttgacca gcttttccag aattttttta cacagtttct
     6241 tcgcgctttt gggttggcgc gcattatagt tcgattggtc aacagtgttt cgattatacc
     6301 aaaggagctc ggcactgtta gcgtccacta atcctatttc cagaaccatc ccagtccact
     6361 gtgagccagc ataggttccg aacaataccg cggacaaagc gccaatagct acatctttgg
     6421 ctttggcgcc acctgaagtt tgatacccca ttcctctcac aaatagaaga taatcggttt
     6481 gtgccaaatc agcaaagacg ccgacattcg ggtcaaccgt aagattaacc gtttttttct
     6541 tgctcactat agcagaatcg caagccttat caaaagactg tttctgtcga gttaagtcca
     6601 aagctaactc ttggtcatat accaaggcag aatcgttcat cggcacggca acagaaacaa
     6661 atcccccttc cttcaaaact tcatttaagg cattgacaat attggagtcc gcttcagcat
     6721 ccagctccgg cttgggttcg tcaatagttg cagtcctctc gaacatatga aattcaggcg
     6781 gcataatccc tatgcttttg atctctttgc gcctctcaga aaagttggga tgagtgcgct
     6841 cggtggtata agcgcatcct acaaagacaa gaagagacaa aagacaaagg gagactaaaa
     6901 acttacgaga ccgcataatt acccctcctg cttcttattg gtttcgggtg tttgtggaat
     6961 aggttcttgt ttgagagatt tgattttaga ttcgatgtat ctttttttct tggtttgtgg
     7021 atccattatc aagtaagttt cgtaatgggt aagcgcttca tttttgtcct tcctggcttc
     7081 ccacacttga gctaatccca gatgtgccag agcaaaagag gaatccaaat ccaaagcctt
     7141 ctggtattgg tcttgtgcta attggagcgt atccggcttg gaaccggaaa gccggtaggc
     7201 ctctccgcac caataatgaa aagttgggtc accagaatag tttacttcaa ttcggctggc
     7261 ttgtttaagc gccttatcgt atttggagct tataacgaaa agctccacgt ttttcaattt
     7321 gaggcggctt accgcttgct cgtatgtgga atcaatttca ggcttaagct cttcaccctt
     7381 atatcctatg gtttcctcga tataacccaa tctctgtttg gtcagggggt gattaccata
     7441 gaagaaattg gcagtggtgg atttgtcagg atactcctca agcagtatct ccaacgaccg
     7501 aacagcctga attttatcat aacctgcttt ttccattaaa tccaatgcgt ttaaatccgc
     7561 ttctttctcg tcagagcggc tatacccgct caccgctgcc atagcagtaa gagccagtcc
     7621 attctgtgcc agggaattcc agaaaccagt catatttgat ccggattgag aaaatcccac
     7681 agccgtagct acgcttaaga attgagcaaa tccagaaacg ttctgaatat gtgcatattg
     7741 acgagaagcg tgacgacgaa gagagtgggt catctcatgg cccaggatga atgctaattg
     7801 gtcgggcttc tccagtttgg ataatagacc aacatgcaga taaatagcac cattgggtaa
     7861 ggcaaaagca ttgagttcag ggtcaaggat tacataaaag cgaaacttga aaatggaatt
     7921 cttatactcc gggggaatca tccgctcccc tatgatctga agatactttg atacggcagg
     7981 gtcggtataa atcagcccgc ttttggtaag cgtattttct tcatatctac tcatatccca
     8041 taacttttta tctcccttgg gaagttgcga aaccgagttc agatctgaaa caactatttc
     8101 cttgccagct gaggcacatc cgattataag cagggtagcc aagagaagaa gcaaaagata
     8161 cgaccttctg taaatcatga tacccccttg gttaggtgag gatttagtgg atgacgagtt
     8221 cccccaagac attctctata tgataaactt tgtccaatat aaacaagaca taacgtatgt
     8281 caacatttat cgaacgattt atttcttctt caaactcata gtccgcagcc atactttcat
     8341 agttgccatc aaaggttaaa cctattaact ctcctttgcc attcattatt ggactacctg
     8401 agttgccgtt agtgccatca ttggtggtga ggaagttaac cggcacatct ccaatggact
     8461 tgtcgaaata tttgccaaaa tctttcttct ggtagatttc ttccagttga attggcacct
     8521 caaatggctc ttttcctgta tttttctgca tgacgccagt caggatagtt atccacttgt
     8581 agaagactgc atcccctggt atgtatccct ggactgttcc ataattaaat cgtattgtcc
     8641 cattagcatc ggggtaagtt agtccttttt tccattcagc ataggcctgg attagtttcg
     8701 gctctaattt cgaaagagcg ccgttaaatc ttttgtcctt ttcttttaag acctcccttt
     8761 ctttctctaa ctctctggca aaatcaataa aggggtcgac gagtttttct aattcctcct
     8821 gggtcatatc gaacatcttc attcgttctt gaagtgttcc aattttggtg ttagcataaa
     8881 gataactaac aaaatcggaa atgacctggt cttgcggcga ccctttcttg ttctcaaaaa
     8941 gtttttccac tacctcgatt ttttgaccag gaggcaactc atgcgccttc ttgaggaaat
     9001 aagccaatat ctttttgtca caagagggaa ccagattgat ttgggcattc tccagattct
     9061 ttctggtctg gggaatctct ctgtccatat actccggctc gcgctccata tcttgttttt
     9121 gcttttctat gctccattta tttatggtgg aggcgagtcc aagaaaatca caatagctaa
     9181 tcatccagct taagatgaaa tccttatcgc gataggtctt gtggtcttca tatagcttct
     9241 ctaaagaagg cagaacatct ccatactttt ggtcaagctg aggattctct ttgagatact
     9301 gagcaagatt cctttcctct tctctcttat gctcaagcac tttggatttc tcaaaccctt
     9361 ccaatacccc ttggttcttt ttaaggtagt tgtaaatgcc tttgatcttg gaggagagtc
     9421 tcaaagcaac tgaggaatct tcctgagaac tttgttccat tatcttgatt aaatcttccc
     9481 tgattccaat atccacgaag taataataat tttgcatatc ttccacagag aaggaactgc
     9541 tatagcgtgc agtccagccg ggaaagccga tcatcatagc gaaatccccc tcctttatgc
     9601 cagcgctgga gactttcaag tagatttctg gacgataagg gacgttgtcc ggtgagtaat
     9661 cagcggattt tccatcacca gctacataag ctctcaaaaa cgagaagtcg ccagtatgtc
     9721 ggggccacat ccagttatct atttccccac cgtattctcc aatagaaaga ggcggcacat
     9781 aaactatgcg cacatccttg attttaaaat aggtgaaaag ataatattgc attcctccat
     9841 aaacacttac cacttcacat ttcacatcct tggcagcttc cgcttcatcg attatctgtt
     9901 tggtcctttt ttcaatggct ttgtatcttt ccaaatcatc catccggtca tcgactgcga
     9961 ccaggacctt atcggtaatt tcatccatag atagggttac atagacatta tacccgatgg
    10021 ccggaatctc ctcggcttta gttttcgcgt gaaatccttc tttcaggtag ttctgtccgg
    10081 gcttgctttg acgctgtatg gcactgaagg caacgtggtg attagttaca atcagtccct
    10141 ccggtgaaac aaaggagccg gatgctccag caacatttac gattgcccgt gcaatgccat
    10201 ccccttttgg attgtaaaca tctttagggc taagttttaa tcccatttct ctcattttgc
    10261 tccaggggag cttatctata gaatcaagca tccacattcc ctcatccgcc aagcaaatcg
    10321 caaaaaacaa cccaagaatg aaaagaacca atagacctaa aaatattctc ttaatcattt
    10381 tttctccttt ttgtattagc gggtagggaa cggttgcacc gttccctaaa tggtaatggc
    10441 gcagccatta cctacccaac tgtattggat taaaataata cccagatata aaatagcaag
    10501 agattaagga tttaggggaa ggtgcaggaa atgacaagga gaatagcgga actttatgga
    10561 aaattgcagg cgtaacttct tagaaagtaa ctgtcagaga aatggtgtgg gtgtttccca
    10621 aaacgccctg cggaacaaag gcgtaatcta tcctgtaact ctcagttcta gcatatagaa
    10681 aaccaacccc aaagctaata ccggacaaga agcccaaatc cgtttcggtc ctgtagccca
    10741 gtctacccat tatcatatga ttgtaaatat attctgcgcc caggtgtaaa ccggtcttgg
    10801 catcggacgg gatgacaaaa tccactgcaa ggttaagggg attgcgtgcg agagaaacag
    10861 tataacagag tccaagtttg gcattgctgg ggagcttgtc gggctcttcg gcaaatttga
    10921 gtgttcctcc ccagttctga aaagacccac ccagaaagag attcttgaac tgagtccggt
    10981 gcaaaaatcc gagatcaaag gcaaaggtgg aagccatttc gtcctcaatt ttctcattta
    11041 tatatttgat gcttcctccg atgaatatat cctttgctat ccgattgcca taaccgagcg
    11101 taattgccat atcgcttgcc ctgaaatccg aagttggctc tccttccacg tcccttccct
    11161 gaagcgggtc catatgcaaa taagccagac tccagccgat tgtcccgtat ttggtgggct
    11221 gagcatgggc gaaatactcg taagcgatat cttgaaacca ccacaggtgc ataaaggaga
    11281 attgggtttt atctatataa gccaagccag ctggattcca atagatggcc gtggcatcat
    11341 tagccagtcc gacaaaggcg ctgcccatac caacagcgcg cgcgcctgca cctatcttga
    11401 ggaattgagc gctgcgcgtg ccgacattgc cacctatggc aaaaccatcg gaggcattta
    11461 tcgctaataa aaccaagacg gctaaaacta ttaataaatt aagcttgctt ctcatttttc
    11521 ctctatctga ttacagccag tttgaagaat ttctcctcac ctttatcatt cgtgattcgg
    11581 catatgtaaa cgccactggc taacttctta ccatcactat taaccacatt ccatacaacc
    11641 tttccatctc cgtctgtctc gtccacagtg taaacccgct cccctacgat ggtgaatacc
    11701 tctattttgg cttgagttgt taagccgtcg aaggtcacaa aggtatgtcc ctggtaaggc
    11761 ttgaaaggat tggggaaaat ggaagcatta gctagggtga gcgcaggacc tggttcgatc
    11821 ttaaaggaat aactcgtatc caattggttc ggaggagagg cgatatcatg cgcgcttact
    11881 cttacaagaa tttcccctcc ccaagggaat tgggttggtg gaaaataggt aaccttataa
    11941 tcgctggggc tcccctcaat tgaagaggaa aaaccaatta tttcctgccc gcctatccag
    12001 agccgtatgg agctcaaatc cacgccatac ccactgtcct ttacatgaaa aacgatattc
    12061 gtatccggtg gaactcgttt agcatttctg ggtggagcaa ctgagtccaa gtatggtggg
    12121 atggaatcga tgaaattgaa agcaaagctg acggttatta ttatctcttt tttgtctggg
    12181 tcttcttgag aaaaggcaag agaaggaaaa atgagacaga caagagaaat gattattaaa
    12241 gttattatcc gaaatctcat cttttcctcg attttgccct atcattcaat ttgtcgtcaa
    12301 aatattgaag gtttttagta ttccttggtg ataaaactcg atatatattc tctgttagtg
    12361 caggaattgg atttcctgca cgagatagct tcgggaattc tcccgccaag cgggatcctg
    12421 gtgacaattc ccgaaccaac taagacgagt ggatgctgaa accagtaaat gctgaaacga
    12481 gttcagcatg acaactgggt ttggtcttac gggttctgtc atcccgaact tggttcggga
    12541 tctcggtttg ggttttaaag cccgccctgt gggcggactt ctttatta
//
LOCUS       DASWLN010000132         9687 bp    DNA     linear   ENV 20-FEB-2024
DEFINITION  MAG TPA_asm: Candidatus Zixiibacteriota bacterium isolate
            SMAG_U2949 k141_1076673, whole genome shotgun sequence.
ACCESSION   DASWLN010000132 DASWLN010000000
VERSION     DASWLN010000132.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37960594
            Sequence Read Archive: SRR10376091
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Candidatus Zixiibacteriota bacterium (soil metagenome)
  ORGANISM  Candidatus Zixiibacteriota bacterium
            Bacteria.
REFERENCE   1  (bases 1 to 9687)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 9687)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (26-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/12/2024 08:28:26
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 1,440
            CDSs (total)                      :: 1,416
            Genes (coding)                    :: 1,412
            CDSs (with protein)               :: 1,412
            Genes (RNA)                       :: 24
            rRNAs                             :: 1 (16S)
            partial rRNAs                     :: 1 (16S)
            tRNAs                             :: 21
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 4
            CDSs (without protein)            :: 4
            Pseudo Genes (ambiguous residues) :: 0 of 4
            Pseudo Genes (frameshifted)       :: 0 of 4
            Pseudo Genes (incomplete)         :: 3 of 4
            Pseudo Genes (internal stop)      :: 1 of 4
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..9687
                     /organism="Candidatus Zixiibacteriota bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_1076673"
                     /isolate="SMAG_U2949"
                     /isolation_source="soil"
                     /db_xref="taxon:2053527"
                     /environmental_sample
                     /country="USA: Oregon"
                     /lat_lon="42.99 N 123.63 W"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            complement(<1..1342)
                     /gene="fliF"
                     /locus_tag="VGB16_04370"
     CDS             complement(<1..1342)
                     /gene="fliF"
                     /locus_tag="VGB16_04370"
                     /inference="COORDINATES: protein motif:HMM:TIGR00206.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0009431 - bacterial-type flagellum basal
                     body, MS ring [Evidence IEA];
                     GO_function: GO:0005198 - structural molecule activity
                     [Evidence IEA];
                     GO_process: GO:0001539 - cilium or flagellum-dependent
                     cell motility [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="flagellar basal-body MS-ring/collar protein
                     FliF"
                     /protein_id="HEX9916952.1"
                     /translation="MPSGKKFTFVFTTIVVVAGLVLLVSWVRNVSYVPLYSGLDASEA
                     GVVVDKLIEGKVPYRLSNRGTTILVASSRVYDIRIKLAAEGLPRTGTIGYEIFDKSNL
                     GMTDFLQKVNYRRALEGELTKTICQLREVQAARVHIVIPERRLFEKDKIEATASVALK
                     LSPAHPLGKSQIQGIAYLVASSVEGLKPGNITIVDYEGNLLSSNNDGNSLASLSGSQM
                     ELRQNVESYLETKAQSLLDGVIGQGKSIVRVTAELNFEQAEKTLEEYDPEKLTIRSEE
                     RSTEGEKENVITNYEVNRTVEHIIGEVGNIKRLSVAVMVDGNYTTTTNEQTGATEKKY
                     IPRTQEELDRLASIVKSAVGFDDTRTDKLEIASVAFDRTKMEDEQKELDKIEKRNNYF
                     SIGEKVTIVIGVLLGLLVLHRIIKKASKSISEIAASAQPPLFKASGKMGVGEKSF"
     gene            complement(1411..1716)
                     /gene="fliE"
                     /locus_tag="VGB16_04375"
     CDS             complement(1411..1716)
                     /gene="fliE"
                     /locus_tag="VGB16_04375"
                     /inference="COORDINATES: protein motif:HMM:TIGR00205.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005198 - structural molecule activity
                     [Evidence IEA];
                     GO_process: GO:0001539 - cilium or flagellum-dependent
                     cell motility [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="flagellar hook-basal body complex protein FliE"
                     /protein_id="HEX9916953.1"
                     /translation="MRTGQISPEQIGIQLPSTKINQTKNTEEIESFGNLLQSFIQDVN
                     SLQSNADQMVQKFALGQVEDMHQVVVASEEANIAFQLLLEIRNKLVDAYHELMRMGA"
     gene            complement(1730..2053)
                     /locus_tag="VGB16_04380"
     CDS             complement(1730..2053)
                     /locus_tag="VGB16_04380"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEX9916954.1"
                     /translation="MKSDAVGIGAYLGSSKVKPMSFREIYPQKEESKIKETIGKEATS
                     DKLELSTAHTDLTGLLSLEEKEFLEKLFGYEESIRNLNSNLKSYTKQHDQNEKILGTK
                     IDIIA"
     gene            complement(2073..2195)
                     /locus_tag="VGB16_04385"
     CDS             complement(2073..2195)
                     /locus_tag="VGB16_04385"
                     /inference="COORDINATES: protein motif:HMM:NF018168.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="flagellar basal body rod C-terminal
                     domain-containing protein"
                     /protein_id="HEX9916955.1"
                     /translation="MPNINVINEIVDMMDASRTHEANLSAMKATEDMTNKALDM"
     gene            complement(2165..2566)
                     /locus_tag="VGB16_04390"
     CDS             complement(2165..2566)
                     /locus_tag="VGB16_04390"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_005008191.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_process: GO:0071973 - bacterial-type
                     flagellum-dependent cell motility [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="flagellar basal body protein"
                     /protein_id="HEX9916956.1"
                     /translation="MTGIFKSMQISASGLSAQRQKMNAVSSNIANIETSKTPEEGPYK
                     RKRVNMSETKEPQNFVDLIQEQTGQLVTTNPRHVSAGANSSFKKAELSEVKAKEEANP
                     DQPVKLVYDPSHPEQTRKDMWLCQILMSSMR"
     gene            complement(2690..3100)
                     /gene="flgB"
                     /locus_tag="VGB16_04395"
     CDS             complement(2690..3100)
                     /gene="flgB"
                     /locus_tag="VGB16_04395"
                     /inference="COORDINATES: protein motif:HMM:TIGR01396.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0009429 - bacterial-type flagellum basal
                     body, proximal rod [Evidence IEA];
                     GO_function: GO:0005198 - structural molecule activity
                     [Evidence IEA];
                     GO_process: GO:0001539 - cilium or flagellum-dependent
                     cell motility [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="flagellar basal body rod protein FlgB"
                     /protein_id="HEX9916957.1"
                     /translation="MEPVKDIITSRTNIPFLKKALNLSSFKQKLTSNNIVNASTPGYK
                     PKDIEFTKELSSFLDKTKTTGTRTNPAHIPLGQNRDKSPEVIATESTDPNNVNSVDID
                     KEMGNLAENELIYRFGTKFMSRNFQLLKLSIKGE"
     gene            complement(3266..4150)
                     /locus_tag="VGB16_04400"
     CDS             complement(3266..4150)
                     /locus_tag="VGB16_04400"
                     /inference="COORDINATES: protein motif:HMM:NF012894.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="flagellar motor protein MotB"
                     /protein_id="HEX9916958.1"
                     /translation="MARKKHEEEHENLERWLLTYADLITLLLAFFIIMYSMSKVDAKK
                     FGAIASAFQGALRGGTSTLKEDTLVTPNDDLGAGPLKVGDLKLLEAEVRRVADELRQK
                     TKISTEVGERGLVIHITESAIFQPGQGRLTPAAMKALDVLAKVIRHVPNDIRIEGHTD
                     NVPIHTTEFPSNWELSTTRATQVARYLIEKHDFSPAKISAMGYAEYRPIFPNDTPENR
                     AKNRRVDIIILSPKKLLDEPYSYQDVVTRKKLVKQVEAGALSEENKTDTLAVQESQSI
                     TEERVPTIVEKFSTYDRN"
     gene            complement(4159..4920)
                     /locus_tag="VGB16_04405"
     CDS             complement(4159..4920)
                     /locus_tag="VGB16_04405"
                     /inference="COORDINATES: protein motif:HMM:NF013760.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="MotA/TolQ/ExbB proton channel family protein"
                     /protein_id="HEX9916959.1"
                     /translation="MDIATVGGLMLGLGAIIIAYLAEGGSISAILKWRAMLLVIGGTI
                     GASTITTSFKTLMTVPKYLKIAFFGKSLDPSELIDRIVKMAERARRDGILELEGDLKT
                     ITNPFFKKAIQLIIDGTEVTVLREILETEMSFVSERHKKGILLFQKMGGLSPTLGILG
                     TILYLISTLANTEDASKMAGHIASALIATLWGIGLANLVYLPLGDKLKHRHEEEMVML
                     ELITEGALSIQSGDSPRVIRTKLLSFVNPSLRKGE"
     gene            5300..6139
                     /locus_tag="VGB16_04410"
     CDS             5300..6139
                     /locus_tag="VGB16_04410"
                     /inference="COORDINATES: protein motif:HMM:TIGR00180.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ParB/RepB/Spo0J family partition protein"
                     /protein_id="HEX9916960.1"
                     /translation="MVVLKDRIEEIEINRIAPLNERRHDKNRIKELAESIENYDLIIP
                     ITVIKEEDGTYSLCKGQGRIEAFKLLNENRIKAFVYNKNEVDRKTVIKWWLIENEVRE
                     KLSEYDKAYLMSLDYDLLGEEETAKKYKLRPTTVRQYKKTMDRITPKIREKVKDGNVS
                     FTLMKETLSAIKDSKSAEEVVDYISEEKLDFEEGRAAIAMAKKLIQENKAVSMRELKE
                     SFRKLSEQNKLLRPVTADLRTKYDFITEWLSILLKDDTFVSLLKEHNQRIPQNILDLN
                     VEV"
     gene            6141..7112
                     /locus_tag="VGB16_04415"
     CDS             6141..7112
                     /locus_tag="VGB16_04415"
                     /inference="COORDINATES: protein motif:HMM:NF014273.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ParB N-terminal domain-containing protein"
                     /protein_id="HEX9916961.1"
                     /translation="MRLHVNDIQLDPQLERRERVEGISKQNDEFLMKSIKNEGLTDHL
                     VVQKVKGEQNKWRLIKGYRRLRALVALDKLGGLPSSTSLSSLPVIEIKDDVNPATLRV
                     ESHRWEDFLPSEKAQRLEELQRKFDMSLKEIANMYGWSTPSVRNWLIINKALPEIKEA
                     IDKGKYPMSAGKVFGTMNEKGQRYLHSKFGDQDNVKRAELWHEWRKIAAKDHLFTLPK
                     KKRVKISQRLVETKPGSKHKHRLIISKEKREKEILNQNIALIQRQNLLFTRDRDDLEK
                     KVFEPLRIVEIWFREDRVREYVKKKYPEIYDGLKAYIGLILNIKIND"
     gene            7440..7754
                     /locus_tag="VGB16_04420"
     CDS             7440..7754
                     /locus_tag="VGB16_04420"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEX9916962.1"
                     /translation="MDTPERESEIKKEIKHYMDENGNHYPYWYVGISEDPENRLFKDH
                     GVKKNGQYAQWIYASAPDSETARRIEDYFVNSLATDGGTGGGDSKAKGVYAYRKYAHT
                     TP"
     gene            complement(7864..8064)
                     /locus_tag="VGB16_04425"
     CDS             complement(7864..8064)
                     /locus_tag="VGB16_04425"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEX9916963.1"
                     /translation="MLGRTSFGISVIPLQEEQRINDPREIPCKDLDFDCFALDGKIPF
                     DDYGKCYAYAPELGRCIFRQSE"
     gene            complement(8064..9419)
                     /locus_tag="VGB16_04430"
     CDS             complement(8064..9419)
                     /locus_tag="VGB16_04430"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_006340408.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_process: GO:0006355 - regulation of DNA-templated
                     transcription [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="sigma-54 dependent transcriptional regulator"
                     /protein_id="HEX9916964.1"
                     /translation="MEKRILVVDDDALMKEFLNETLTRKKYSVDLASTGEEALKMMKQ
                     KEYDVILSDIRMPQLGGMDVLKATKSFLPDAKVILMTAYATVENAVEAMKLGAYDYIM
                     KPFSADAIEVAVEKAFEHKRLVLENRILKSQIAHQYRFENIVGKSPQMQKVFEMVDVV
                     ANSRATVLITGESGTGKELIAKAVHYNSPRKDGPFIKINCAALPENLVESELFGHEKG
                     AFTGAIRQTRGRFELADGGTLLLDEISEISLGLQAKLLRVLQEREFERVGSGQSIQVD
                     VRIISTSNQNIPELIRKGKFREDLYYRLNVIPIHLAPLREKKEDIPALAERFLRIYNE
                     DNKRSIEGISEKVYELFMNYHWPGNVRELENYIERAIVMTKGKILTPSEFPRELALGQ
                     TKTTLDSIEVGCSIDEAEKKLILKTLDAQSDNRTKAAEILGISTRTLRNKLQEYGLKE
                     N"
     gene            complement(9429..>9687)
                     /locus_tag="VGB16_04435"
     CDS             complement(9429..>9687)
                     /locus_tag="VGB16_04435"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_012519961.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=2
                     /transl_table=11
                     /product="ATP-binding protein"
                     /protein_id="HEX9916965.1"
                     /translation="KSKDGVGISEEKIILEISDCGVGMNKETQAKLFTPFFTTREEGT
                     GLGLSTVKKIVEAHRGDIYIQSELGKGTKVRVILPKEITLV"
BASE COUNT         2744 a         2136 c         1941 g         2866 t
ORIGIN      
        1 tgaaactctt ttcccctacc cccatcttac ctgacgcttt aaataaaggt ggctgagctg
       61 aagccgctat ttccgatatg ctcttggatg ctttctttat tatccgatgc aggactagaa
      121 gacccagtaa aactcctata actatcgtta ccttttcgcc aatggagaaa taattattcc
      181 tcttctctat tttatctaac tccttctgct catcctccat tttggttcgg tcaaaagcga
      241 ctgaggctat ctccagctta tccgttctgg tatcgtcaaa gccaactgca cttttaacta
      301 tgctagctaa tcggtctaat tcttcctgtg tacgagggat gtatttcttc tccgttgctc
      361 cggtttgctc attggtggtg gtagtgtaat tcccatccac catcaccgcc acggacaaac
      421 gtttgatatt tccaacctct ccgatgatat gctcaactgt tcgattgact tcgtaattgg
      481 ttatgacatt ctctttttcc ccttcagtgc tcctctcctc gctgcgaatg gtaagctttt
      541 ccgggtcgta ttcctctagg gttttttcgg cctgctcgaa attaagctca gccgtaaccc
      601 tgacgatcga tttgccttga ccgataactc catccagaag ggattgtgct ttggtttcca
      661 gataagattc aacgttttgt ctcaactcca tttgacttcc cgaaagagac gccaaactgt
      721 ttccatcatt attggaggag agaaggtttc cttcgtaatc cacaatggtg atattgcccg
      781 gtttcaaccc ttcaacgctg gatgccacca gataggctat accttgaatc tggctcttgc
      841 ccaatggatg agcaggactg agcttcaaag ctactgaggc ggtggcttca atcttatcct
      901 tttcaaaaag ccgcctttca ggaatgacga tatgtactct tgccgcctgt acttctctca
      961 actgacagat ggtcttggtt aactcccctt ccagggctct acgataattg accttctgca
     1021 ggaagtcagt cattcccaaa ttgcttttat caaaaatctc atatcctatt gttcctgtac
     1081 gaggcaatcc ttcagctgcc agctttatcc ttatgtcata cactcggctg gaagccacca
     1141 ggatagttgt tcctctgttg gataatctat aaggcacttt tccctcaatt aacttatcta
     1201 ctactacacc agcctcactc gcatctaagc cggaatagag cggaacgtaa ctgacatttc
     1261 tcacccagga gacaagaagg accaatcccg ctacgaccac aattgtggtg aagacaaaag
     1321 taaacttttt gccggagggc aaacctctcc aaaaattaga gaattgcgta aagagtttcc
     1381 ttaaaaaatc catttttcac ctcgatttgt ttaagctccc atgcgcataa gctcgtggta
     1441 agcatctact aatttattcc ttatctccag aaggagctga aaagctatgt ttgcttcctc
     1501 tgaggctacc accacttgat gcatatcttc aacctggccc agggcaaact tctgcaccat
     1561 ctggtcggca ttgctctgca aactattgac gtcctgtatg aagctctgta gtaaattgcc
     1621 aaagctttca atctcctcgg tattcttggt ctgatttatc ttggtggagg gaagttgaat
     1681 tccaatctgt tcgggcgata tctgtccggt tctcatcgat taacctcctt caagcgatta
     1741 tgtctatctt agttcccagg attttctcgt tttggtcatg ttgtttagta taggatttaa
     1801 gattactgtt caaattcctt atgctctctt catatccaaa aagcttttcc aaaaactcct
     1861 tctcctctaa ggagagaagc cctgtcaagt ccgtgtgggc agtggataac tctaatttgt
     1921 cagaagtagc ctcttttcca atcgtctctt ttatttttga ttcttccttc tggggataaa
     1981 tttccctgaa agacattggt ttaacttttg agctcccaag ataagctcca attcctactg
     2041 catcgctctt catagttcat gctcctgtgg gtctacatat ctaaggcttt attagtcata
     2101 tcctcagtcg ccttcatagc agagaggttt gcttcatgag tcctggaggc atccatcata
     2161 tccactatct cattgatgac attaatattt ggcaaagcca catatccttt ctcgtctgct
     2221 ctgggtggga tgggtcgtag accaacttaa ctggctggtc aggattggct tcttcttttg
     2281 ctttgacttc ggaaagttct gctttcttga aagaactatt ggctccagcg gaaacatgtc
     2341 tcgggttggt agtaacaagt tgaccggttt gctcctgtat aagatccaca aaattctggg
     2401 gttccttggt ctctgacata tttactctct tgcgcttgta cggtccttcc tccggagtct
     2461 tagaggtctc gatgttggcg atatttgagg aaaccgcatt cattttttgc ctctgggcgg
     2521 aaagcccact ggctgaaatc tgcatacttt tgaaaattcc ggtcataaac cctcactttt
     2581 tgcatactca atcgggtaag taatggctgc gccattactt ctactgtctt ttatcttttc
     2641 gggaacggtg caaccgttcc ctacccgttt ctttatagta agctttgttt tattctccct
     2701 taatacttaa cttcaaaagt tggaaatttc ggctcatgaa tttagttcca aatctataaa
     2761 tcaattcgtt ctcagccaaa ttccccatct ccttatcaat atccacgcta ttgacgttgt
     2821 ttggatcagt tgattccgta gctattacct caggggattt gtctctgttc tgacccaatg
     2881 gaatgtgagc aggattagtg cgcgtgccgg tggtcttggt tttgtccagg aaagaactga
     2941 gctctttggt gaattctata tctttaggtt tgtacccggg agtagaagca ttgacgatat
     3001 tgttggaagt cagcttctgc ttaaaagaag aaaggtttaa agctttcttc aggaagggaa
     3061 tattggtacg gcttgttatt atatctttta ccggctccat cctgacctct atggagtttt
     3121 aaagttaaaa cagagttttc acctggcaaa atttttagca aatattgtgc caagagctcg
     3181 cttgagtatt tattggagat ttaacttatg gattggattg aagttgtctt gaaagtcaat
     3241 ctgcggtgat tgcatttgta tcggtctaat tccggtcata agtggaaaac ttttccacaa
     3301 ttgtaggcac tctttcctcg gttatgctct gtgattcttg caccgccaag gtatccgttt
     3361 tgttctcctc ggataatgcc ccggcctcta cctgtttaac caattttttc ctagtcacaa
     3421 catcttgata agaatagggc tcgtctaaca gcttttttgg agaaagaatg atgatgtcca
     3481 ctcttctgtt tttggctctg ttttctggag tgtcgtttgg aaaaataggt ctgtattcag
     3541 catatcccat agcagaaatc ttggcaggtg agaaatcatg cttttcaatt aagtatctcg
     3601 ccacctgagt agctctggtt gtggaaagct cccagttgga aggaaactct gttgtatgaa
     3661 tgggaacatt gtccgtgtgc ccctcgattc ggatatcgtt cggtacgtga cgaatgactt
     3721 tggctaaaac gtctaatgcc ttcatggccg caggagtcaa acgcccttgt cccggctgaa
     3781 aaatggcact ctccgtgata tgaatgacta atcctctttc ccctacctcc gtgcttatct
     3841 tggtcttttg tctcaattca tccgcaaccc tccgcacttc ggcctcaaga agtttcaaat
     3901 ccccaacctt aagcggtcct gctcctaagt catcattggg cgttactaag gtatcttctt
     3961 ttaacgtgct tgttccaccc cgtaacgcgc cctgaaaagc cgaggctatt gctccgaatt
     4021 ttttagcatc tactttggac atggaataca taatgatgaa gaaagccaga agtaaggtta
     4081 ttaaatcagc gtaagtcaaa agccagcgct ccagattttc atgctcttct tcatgctttt
     4141 tgcgagccat cttggaacct attcaccttt tctcaagctg ggattaacaa aagacaaaag
     4201 cttggtgcga atcactctcg ggctatcccc ggattgaata gacagagccc cttcggtaat
     4261 caattccagc atcaccattt cctcctcatg gcgatgtttg agtttgtcac ccagcggcag
     4321 atataccagg ttcgccaaac caattcccca taaggtggcg attaaagcac tggcaatatg
     4381 tcctgccatc ttactggcat cctcagtatt tgccagagtg ctgatcaagt agagaatcgt
     4441 accaaggatt ccaagagttg gagaaagtcc tcccatcttt tgaaataaca aaatcccctt
     4501 cttatgcctt tctgaaacga atgacatctc ggtctccaat atctctctca gcacagttac
     4561 ctcagttcca tcaataatca attgaatggc tttcttgaaa aacggattag tgatagtctt
     4621 caggtcacct tctaattcca atattccatc ccttctcgct ctctctgcca ttttgacgat
     4681 tctatctatc aactctgagg ggtcaagtga ttttccgaag aaggctatct taagatactt
     4741 gggcacagtc atcagggtct taaaggaagt agtaatggtg gaggcgccga tggttccacc
     4801 tataaccaaa agcatggctc gccacttcag gatggcggat atactaccac cttctgctaa
     4861 ataagctatt ataattgcac ccaacccaag catcaaccca cctacagtag ctatatccat
     4921 acttctttcc tcgtagtctt tagaagatag tatcccttta gcgtataggt atacctatcc
     4981 cttttgggtt tacacctatt catcgtaagt atcggcaagg ttcacttggt tctaaacttc
     5041 aaacttttga actaacttta ttttgttgca cagcgggacc cgacaggatg accgacacct
     5101 caatatgaac atcatgattc ataccgagga ataacatttt cggtttttgc ggagtctgag
     5161 tatgagaaga gagaaagaaa tacggtgtta atttttttag aaaaagtact tgacaaaggt
     5221 accacaatga tgtacactta agtcgtacac gaaattgaaa ataataacaa aaaggtaggt
     5281 accaaaaaag gaggttgaaa tggttgtctt gaaagatcgt atcgaagaaa tagaaataaa
     5341 cagaatagca ccattaaacg agaggagaca tgacaagaac aggattaagg aactagcaga
     5401 aagtattgaa aactatgacc taatcattcc tattacagtt atcaaagaag aagacggaac
     5461 ttatagcttg tgtaaaggtc agggtagaat agaggccttt aagcttctta atgaaaatag
     5521 aataaaggca tttgtctaca acaaaaacga agttgacaga aaaacagtaa ttaaatggtg
     5581 gctgatcgag aatgaggtta gagaaaaact ctcagaatat gataaagcat atcttatgtc
     5641 tttggattac gatctgctcg gcgaagaaga gacagcaaag aaatataaac ttagacctac
     5701 cactgtccgg caatacaaaa aaactatgga cagaatcact cccaaaatca gagaaaaagt
     5761 taaggatggc aacgtctcct ttactcttat gaaagagacc ctttctgcca ttaaggactc
     5821 taagtcagcg gaagaagtgg ttgattatat ttcagaagag aagcttgatt ttgaagaagg
     5881 tcgggcagca atagcgatgg cgaagaaact aattcaagaa aataaagctg tctcaatgag
     5941 ggaactcaag gagagtttta gaaaactgtc tgagcaaaat aagcttcttc gtccagtaac
     6001 ggctgattta agaacaaaat acgatttcat aacggaatgg ctgtcgatct tgctaaaaga
     6061 cgatactttc gtatctctat taaaagaaca taatcaaagg ataccacaaa atatattaga
     6121 tttaaacgtg gaggtgtaaa atgagactgc atgttaatga tattcaatta gatccacaat
     6181 tagaaagaag agaaagagtt gaaggaatct ccaagcagaa tgatgaattt ctgatgaagt
     6241 ctattaagaa tgaaggacta acagaccatc tcgtggtgca aaaagtcaaa ggagaacaaa
     6301 ataaatggcg actaattaag ggctatagac gactaagggc gcttgttgct ttggataaat
     6361 taggcggtct accttccagc actagcttaa gttctttgcc tgtaatcgaa attaaagacg
     6421 acgttaaccc ggccacccta cgcgttgaat ctcatagatg ggaggatttt cttccgagcg
     6481 aaaaagccca aaggcttgag gagttacaga gaaaattcga catgtcactt aaagaaatag
     6541 ctaatatgta tggttggagc actccctcag taagaaactg gctcatcata aacaaagctc
     6601 tccccgagat aaaggaagca atagacaaag ggaaatatcc aatgagtgca ggtaaagtct
     6661 ttggaacgat gaacgaaaag ggacagagat acttacatag taaatttggg gatcaagaca
     6721 acgtaaagag agcagagctt tggcacgaat ggagaaagat agctgcgaag gaccacttgt
     6781 ttacattgcc caagaaaaag agagtaaaga tttcgcaacg cctagttgaa acgaaacctg
     6841 gctccaagca taagcataga ttaattatca gcaaagagaa gagagaaaaa gagatattga
     6901 atcagaatat agctttaatt cagcgacaga atttgttatt tactcgagat agagatgatt
     6961 tagaaaaaaa ggtttttgaa cccctaagga ttgtggaaat ctggtttagg gaagatcggg
     7021 tcagagaata tgttaaaaag aaatacccag aaatctacga tggcctaaag gcatacatag
     7081 gtttaatatt gaatataaaa ataaacgatt aaaaagatag gtaccatatc aacgtacctt
     7141 cagttaaata aatctccaga tttctggaga ccaacatgaa aagccttgca ggaaccattc
     7201 ggacaccgga tgttgctata tcaaccgtaa gtattaatga aaccttcaaa ggaaagagtt
     7261 aaacagtgat agagatcaag agataaatcc ccgaccgtcg ctccggtcgg ggattaacga
     7321 atcgatcgag tccactaata aagactcaat caagcctaat ttgtaattaa ctagtgattc
     7381 gcgaattgtc aagaaaaaac tgtgaagata aataatgaaa ctcatggagg ttgattatta
     7441 tggatacacc tgaaagagaa tcagagatta agaaggaaat aaagcactac atggatgaaa
     7501 atggcaacca ctatccctat tggtatgtag gtatttcaga agatccagaa aatcggcttt
     7561 ttaaagacca cggggtaaag aaaaatggcc agtatgcaca gtggatttac gcttctgcac
     7621 cggattctga aactgccagg agaatcgaag attattttgt caatagttta gcaacggatg
     7681 gcggtactgg tggtggtgac agcaaagcaa aaggagtcta cgcatacaga aaatacgcac
     7741 atactacccc atgaacgact aaactaatat ttaaacaccc cacccgactt gggtggggtg
     7801 tttttttgtt tcgagtagag tgggagattt tttgttgaca aaccttctat ataggatgga
     7861 attttattca ctttgacgaa aaatgcatct ccctaattcc ggggcataag cgtagcactt
     7921 gccataatca tcaaaaggga tttttccatc taaggcaaaa caatcgaaat ctaaatcttt
     7981 gcagggaatt tcgcgggggt catttatcct ttgctcttcc tgaagaggta ttacagatat
     8041 tccaaaggag gttctgccta acactaattc tcctttaacc cgtattcctg caacttattt
     8101 ctcaaggtgc gggtggagat gcccaaaatc tcagccgcct tggtgcgatt gtcactctgg
     8161 gcatccaacg ttttcaaaat caatttcttc tccgcctcat cgatgctaca acccacttca
     8221 atggagtcga gagtagtttt ggtctgtcct aaagccaatt ccctggggaa ttcactcgga
     8281 gtgagaattt tccctttagt cataactatt gcccgttcga tataattctc caactcgcgc
     8341 acattcccgg gccaatgata attcatgaaa agttcatata ctttctcgga tattccttca
     8401 atcgaacgtt tgttatcttc attatatatt ctcaggaaac gttcagccaa agccgggata
     8461 tcctctttct tctctcttaa gggggcaaga tgaatgggga taacgttcaa acggtaatat
     8521 aggtcttccc tgaatttccc ctttcttatt aattcgggta tgttctggtt ggaagtggag
     8581 atgattctga cgtcaacctg aattgactga cccgaaccca ctctctcaaa ctccctttcc
     8641 tgcaaaaccc tcaaaagttt cgcctgaagc cccaaggaga tctcacttat ctcatctaga
     8701 agcagggtgc ctccatctgc cagctcaaat cttcccctgg tctgtcttat cgctccggtg
     8761 aaagcccctt tctcatgtcc gaacagttca ctttccacta gattttctgg caaggcagcg
     8821 cagttaattt tgataaaagg accatctttt ctggggctgt tataatgaac ggctttggcg
     8881 attagttctt tgccggttcc ggattctccg gtaatcagga ctgtggcgcg gctgttagct
     8941 accacatcca ccatttcaaa gaccttctgc atttgaggac tcttgccaac gatattctca
     9001 aagcgatact gatgggctat ctgggattta agaatcctat tctccagaac caatctctta
     9061 tgttcaaaag ctttctcaac agctacttca atcgcgtctg cggaaaacgg cttcataata
     9121 taatcgtaag cacccaattt catcgcctcc accgcgttct ccactgtggc ataggcggtc
     9181 ataagtataa ccttcgcatc tggaagaaag ctcttagttg ctttcaacac atccattccg
     9241 cccagctggg gcatacggat atcggaaagg ataacatcat attccttctg cttcatcatt
     9301 tttaaagctt cctctccggt ggaagccaga tcaacgctgt atttcttgcg ggtgagggtc
     9361 tcgtttagaa actctttcat taaagcatca tcatccacta ctaaaattcg cttctccata
     9421 gattcccctt aaaccagagt tatctctttg ggtaatatca ctctgacttt cgttcccttg
     9481 cccagctcac tttggatata tatgtcgccc cgatgggctt ctacaatctt ttttactgtg
     9541 gaaaggccta atcctgttcc ctcctccctg gtggtgaaaa acggggtaaa gagtttagct
     9601 tgtgtttcct tgttcatccc cacaccacaa tccgagatct ccaagatgat tttctcctcg
     9661 gaaattccaa ctccatcttt ggacttg
//
LOCUS       DASWLN010000133        10440 bp    DNA     linear   ENV 20-FEB-2024
DEFINITION  MAG TPA_asm: Candidatus Zixiibacteriota bacterium isolate
            SMAG_U2949 k141_1196082, whole genome shotgun sequence.
ACCESSION   DASWLN010000133 DASWLN010000000
VERSION     DASWLN010000133.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37960594
            Sequence Read Archive: SRR10376091
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Candidatus Zixiibacteriota bacterium (soil metagenome)
  ORGANISM  Candidatus Zixiibacteriota bacterium
            Bacteria.
REFERENCE   1  (bases 1 to 10440)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 10440)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (26-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/12/2024 08:28:26
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 1,440
            CDSs (total)                      :: 1,416
            Genes (coding)                    :: 1,412
            CDSs (with protein)               :: 1,412
            Genes (RNA)                       :: 24
            rRNAs                             :: 1 (16S)
            partial rRNAs                     :: 1 (16S)
            tRNAs                             :: 21
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 4
            CDSs (without protein)            :: 4
            Pseudo Genes (ambiguous residues) :: 0 of 4
            Pseudo Genes (frameshifted)       :: 0 of 4
            Pseudo Genes (incomplete)         :: 3 of 4
            Pseudo Genes (internal stop)      :: 1 of 4
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..10440
                     /organism="Candidatus Zixiibacteriota bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_1196082"
                     /isolate="SMAG_U2949"
                     /isolation_source="soil"
                     /db_xref="taxon:2053527"
                     /environmental_sample
                     /country="USA: Oregon"
                     /lat_lon="42.99 N 123.63 W"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            <1..1457
                     /locus_tag="VGB16_04440"
     CDS             <1..1457
                     /locus_tag="VGB16_04440"
                     /inference="COORDINATES: protein motif:HMM:NF017044.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0046872 - metal ion binding [Evidence
                     IEA]"
                     /codon_start=3
                     /transl_table=11
                     /product="pitrilysin family protein"
                     /protein_id="HEX9916966.1"
                     /translation="HFYGMLKAGTIVNAGYEFLENYIPNLEKVQPKEIQRVAKKYLYK
                     APYLATVNAPLSNEEKKEETKTTSSYRREVLPNGLTVIVKSNPDSKVLAMNVLGKNRS
                     IMEPEGKEGIVDFVNRMLLKGTKNRTAEELSNDLALVGAQITNVDNPYIPYDDRYTTK
                     SFTFFRFETIDEYADKGIDILADIIKNSNFPQDQIEKVKAEMMGILGMQGGSTYQTAR
                     DLFYAELFKGHPFSKTINGNRRSIGGITQEDLIQFYKKFYSPNNIIITAATSLPVDEM
                     MNKIKLYFGDMPKVDFAFPDIPLPSKPTSPSKVEKKMEKEQVYIYLGSLLPGIESQDM
                     PAIEVTNEILSSRLAQELREKQGLAYSVGSSTNFEKGFGWFVASIGTRPQNREIALNG
                     ILEEMKKIKRELPSSEELEKAKNSIWGSLLMGRLSRINQAHYMGVYEFEGLGYDYDEK
                     YIKQIREVTAEEIKQAAIKYLDTENYVLAVVGKI"
     gene            complement(1986..2471)
                     /gene="coaD"
                     /locus_tag="VGB16_04445"
     CDS             complement(1986..2471)
                     /gene="coaD"
                     /locus_tag="VGB16_04445"
                     /EC_number="2.7.7.3"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_014856135.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004595 - pantetheine-phosphate
                     adenylyltransferase activity [Evidence IEA];
                     GO_process: GO:0015937 - coenzyme A biosynthetic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="pantetheine-phosphate adenylyltransferase"
                     /protein_id="HEX9916967.1"
                     /translation="MRRAVYPGTFDPMTNGHLDLIKRALNIFDELTIAIAENPTKKPL
                     FSKKERLEMVKASVGNLRRVQVVPFSGLLADLTKELKAVAIIRGVRAVSDFDYEFQLA
                     LTNRKIAPKAETVFLMPSEKYSYLNSTLIKDIAKWNGDTSKFVPPLVAKRLREKFGYK
                     V"
     gene            complement(2487..3041)
                     /gene="rsmD"
                     /locus_tag="VGB16_04450"
     CDS             complement(2487..3041)
                     /gene="rsmD"
                     /locus_tag="VGB16_04450"
                     /EC_number="2.1.1.171"
                     /inference="COORDINATES: protein motif:HMM:NF015559.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0052913 - 16S rRNA
                     (guanine(966)-N(2))-methyltransferase activity [Evidence
                     IEA];
                     GO_process: GO:0031167 - rRNA methylation [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="16S rRNA (guanine(966)-N(2))-methyltransferase
                     RsmD"
                     /protein_id="HEX9916968.1"
                     /translation="MRIVGGKLKSRRLFVSSKANFRPTADRVKESIFNILKGELEGKR
                     VLDLFAGTGNLGIEAISRGAISITFVDFSAQSTKIIQRNLGDLECLSCAEILKLDYRK
                     ALQKLAREGRKFEIVFADPPYLLGYAQGVIDYLKETGILGVNALVIIEHHKKEELNLD
                     SSDLVELRRRKFGDTIVSILSKKR"
     gene            complement(3054..3464)
                     /locus_tag="VGB16_04455"
     CDS             complement(3054..3464)
                     /locus_tag="VGB16_04455"
                     /inference="COORDINATES: protein motif:HMM:NF018247.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="helix-hairpin-helix domain-containing protein"
                     /protein_id="HEX9916969.1"
                     /translation="MKWWDFNLAEMRGLLVLLVLLLVGSGIAIYKKYHANFPMEIIFR
                     ESKAERGQEKTTKTIQHTQSKIASPAPTEKVDLNTTSWAELDLLPYIGPTLSKKIIEY
                     REKNGKFKQVDDLLKVNGIGEKTLEKIRGYVEVK"
     gene            complement(3472..4362)
                     /locus_tag="VGB16_04460"
     CDS             complement(3472..4362)
                     /locus_tag="VGB16_04460"
                     /inference="COORDINATES: protein motif:HMM:NF016289.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="M28 family peptidase"
                     /protein_id="HEX9916970.1"
                     /translation="MLNTKRITLFLLTFFLCCSSVHLPSFDRDRAFQYLLEQCEFGPR
                     NPGSINHSKTKNYLLDKLSQGTNLVRMQNFVYHDSLNNSDLNLTNIIASFYPEETKRV
                     LLGAHWDTRPSADKELDSSLRDKPIPGANDGASGVAVLLEMANVLSKSKPKYGVDIVL
                     LDGEDMGKEGSDDFCLGSKYFAKNLGDYRPQFGIILDMIGDKNLNIYPEGYSVAYAPK
                     VVDLVWSRARKLNYQCFGSSVKHTLIDDHLPLISAGVPCIDVIDFDYSYWHTLSDTPD
                     KCSSESLEKIGKLLIEVLYH"
     gene            complement(4367..10189)
                     /gene="sprA"
                     /locus_tag="VGB16_04465"
     CDS             complement(4367..10189)
                     /gene="sprA"
                     /locus_tag="VGB16_04465"
                     /inference="COORDINATES: protein motif:HMM:TIGR04189.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="cell surface protein SprA"
                     /protein_id="HEX9916971.1"
                     /translation="MYSSEQKPVTAKMVLLVLSLAVLLFHIPTPVYAQGLDFNLNLSY
                     PETFYRSDLAQKKGLSMAFPTAPLLERKLQLQKEVKIDYDLGWVELSTKAGNYEIGQD
                     LVLDLPDYVSQVSASETQKLWLGEQGKTMEVSEQGGKKDLLGFEIPVKFPKAISKIVG
                     EGGPGLKVNGYKRISFAGRSQWQDGFTNTATSRQSKFPSLEMEQDSRFTITGTIGSKI
                     TVKVDQDSKRMTDLENNIQLRYNGEEDEVIKSIELGNTSWSIPGLAGYSGTIKGLFGA
                     KATAQVGGLDMTVVASQEKGSTEKAIFQAGAQVTPQFIRDYEFLRWTYFYLGHSKDLD
                     TSHTVNDFNKGDSIIDTSLVVFENTTGSGGTDLFGYACVNPRDTSEYPGEAYNRRFHL
                     VPDSTYRWGITQDSTNFWIKFNPRREEAAIVAIRYKKIPAGYPGSIIEVGHLATKIGN
                     DITDTLKLIKAEYPKPSFITWAYEWKNVYDLRGRDIDKEGFELRIYKGSGTDIEADKD
                     YDSSGAYITILGLDSLNLKGEPIPDRSVDIVDTKINFHEGLLIFPMKKPFAYEGLSVK
                     VPSIYDNSNNNDRMDASQYYIYTKSASRSTQYSLGRTSIIEGSEIVTLNGERLTRGVD
                     YNIIYEIGQITFTNDKVLDPTANLTIDYEYAPLFMLEKKTLFGLNTNYDMGNLKMGLS
                     ALYKSEKTGEDRPRVGQEPTRNFIWDGNLQFNSSPLVMTKLADALPLVETDAPSNLTF
                     SAEIAQSLPNPNIKNDAFIDDFEGSLEYTDLGIRRGTWTLSSMPTGRDLIQRGRMIWY
                     NPYDGYYVKDIWPAMETTAEDQKTHILVLKLDSSSPHIPADTSFVPNVNVWNGIMRAL
                     PKGSYDQSKTEFLEIWLNGDKGKIHIDLGQVNEDIDNNGVLDTEDSTRNGFKDGILQD
                     DEDKGLDHWWDDEERSKYDTSLSDPSGDNWSYDPNVDKNNYSQINGTQGNASDPDRGR
                     YPDTEDLNRNDVPDVSNNYFEFSIDLVSDAPVEVSGQNWKLYRIPLKGEGNYDTVGAP
                     GWSDIRFARVWVEDCDSIRIASFQLVGNRWKSSGTSPIDSLPVEGGGSFKIFVINTHE
                     NPEYSSPPGVAGEEDKNTGVRQKEQSLVLKYENLKPGYYGAAYRTLYQVEDYTNYKDI
                     KMYVHGPDIHAPSDTAGLEFFFRIGTDSVNYYEYHTNLYPGWDERNYVAINFEEVTRI
                     KSYMLEEPPDSEGYDITQEHYRIRGKPTLSQVDWFSMGVENDTLSAEVKSGEVWVDEL
                     RVADVRKNPGWAIRSSASFNFADLLSVSLSLNKTDSEFHDLRTNRGSGVNSTGTSFGT
                     SINLEKFLPPSWGLDLPFSYSWSKNLSVPRLKVGSDIVLPVEQRDQEKTESVNEAFSF
                     SPSFKKNTKNWITNWTLNRISTRMSYSKQKGRSPATPVSNSSNYQVTGGYDLAPPSKL
                     SFSPLTWSKKVFLLNKLSQTKFYYLPTALNFDGRVNRLKSYSLNDFGSPTSVYTRDFD
                     GGMNLAFSPFSNLPLGYTWRTNRDIRDGNQIKFSLNPSKMRLGIERTNRKSYTASYTP
                     RIFSFLDHRFSFNSNYNENCDPLQFTDGTRSVTNDNTKSASFTLRWQKLLEKVSLEDK
                     PKSTSADKKETKSGPLSWIRKRIGFLSKRINPLQGSVSRNKSFTRSGLLDRPSFRYQL
                     GFTDDPGVGMKPTTSGTINDALTTRDNYSLRSGIAILSTNLDISYSETITDTRNPNAH
                     TENRSKTFPELSFGWNNINKFKFLNKYVSSLGFRFGYSRKVGTTKDKLVDATKSKNTL
                     ISFNPLASFSLVWKNGIRTNLGIDKSQNKNEDLGFQGGNRTTTVDSDQTVILNNSYSF
                     RAPQGIKFPLLKKIKFESNLSLSLDISYRTHKQKKSTEDNPSNLIQHRTELNISPRAG
                     YNLSSQVQAGLSGTWMDSNDKITKIKSHTRQLGIWFEIRF"
     gene            complement(10259..>10440)
                     /locus_tag="VGB16_04470"
     CDS             complement(10259..>10440)
                     /locus_tag="VGB16_04470"
                     /inference="COORDINATES: protein motif:HMM:NF014872.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=3
                     /transl_table=11
                     /product="UDP-N-acetylenolpyruvoylglucosamine reductase"
                     /protein_id="HEX9916972.1"
                     /translation="VGAKNLCVGDAAIYDKHANIVVNLGKARAKDVKSLADILKRRVK
                     ERFKIKLEEEVVFLG"
BASE COUNT         2808 a         2332 c         2122 g         3178 t
ORIGIN      
        1 ttcattttta cggaatgctc aaagccggca caattgttaa tgccggatat gaatttctgg
       61 aaaactatat tcccaatttg gaaaaagttc agcccaagga gattcaaaga gttgccaaaa
      121 aatacctcta caaagctcca tatcttgcta cagtgaatgc tcctctttcg aatgaagaaa
      181 aaaaagaaga aaccaagaca accagctcat acagaagaga ggtattaccc aatggcttga
      241 ccgtaattgt gaaaagtaat cccgattcaa aagtgttagc catgaatgta ttggggaaaa
      301 atcgctctat tatggagcca gaaggaaaag aggggattgt tgattttgtc aatcgtatgc
      361 ttctgaaagg aaccaaaaat agaacggccg aagagttatc caacgatttg gctttagtgg
      421 gagcacagat aaccaatgtg gataatcctt atatacctta tgatgaccgt tacaccacca
      481 aaagctttac cttcttccgt tttgaaacca tagacgagta tgcggataag gggattgata
      541 tcttggctga tatcataaaa aactctaact ttccgcaaga ccagatagaa aaggtcaaag
      601 cggagatgat ggggattttg ggaatgcagg gcggtagcac ttatcagact gccagagacc
      661 tgttttatgc tgaacttttc aaagggcatc ctttctctaa gaccataaat ggaaacagga
      721 gaagcattgg tggaataaca caggaggatt taattcaatt ctataagaaa ttttattctc
      781 cgaataacat aattattact gctgctacaa gtttgcctgt agatgagatg atgaataaaa
      841 tcaaacttta tttcggagat atgccaaaag tcgatttcgc ctttccggat attcctcttc
      901 cgagcaagcc aacttcccct tccaaagttg agaagaaaat ggaaaaggag caagtttata
      961 tatatctggg ttctcttctg cctggaatag aaagccagga tatgccggca atagaggtta
     1021 ccaatgaaat cctttcatct cgattagccc aggagctcag ggaaaagcag ggtttggctt
     1081 attcagtggg ctcatccacc aattttgaga aaggctttgg ttggtttgtg gcttctattg
     1141 gaacccgacc gcaaaatcgc gaaatcgctc tgaatgggat tttagaggag atgaaaaaaa
     1201 ttaaaaggga gttgcccagt tcagaggagt tggaaaaagc caaaaattca atttggggaa
     1261 gccttcttat gggacgcctc tcccgcataa accaagccca ttatatggga gtatatgaat
     1321 tcgaggggtt gggttatgat tatgacgaga aatatatcaa gcaaataaga gaggttactg
     1381 cggaagagat taaacaagcg gcgataaaat atttggacac tgaaaattat gttttggctg
     1441 tggtggggaa gatctgaaga tatggatata ccttatgtaa gaggtgttta ttttcgtttg
     1501 tcattcctgt gctatgaaac gttatggttt atagcggcgc aaacaggaat ccattgattt
     1561 ttttgtgaat ccatatggag tgcattgacc atgtcaatgc tgcatccatt tggatgcact
     1621 ccaaaaaaga tgttttatat tcgtaggggc gggaattcat cccaccccga tttttcatag
     1681 gcgacccata tgggtcgcct tcatatctaa ttcattgtag ttgcccaatt tattgggcga
     1741 atcttgaatt tacgtagggg tagagttcat atctaccctt gcttttcggg cgaagtcaag
     1801 cttcgcccct acgcgaacat aaaataatcg tagctgacat tcccggaatg tccgctacgt
     1861 tattctgcat tattgtaggg gtcggattcc ctggccatgg aggtcgagcg ggaagagacg
     1921 ggcaggcatc cgacccgtcg ggcttgatga atccctggtc gggcgggcaa gccactacga
     1981 attctctaaa ctttataccc gaacttttct cttaacctct ttgccaccaa agggggaacg
     2041 aacttgctgg tatccccatt ccacttggca atatctttta ttaaagtgga gttgagatag
     2101 ctatacttct cactcggcat cagaaagact gtttcagctt tgggggctat ttttctgtta
     2161 gtcagagcca attgaaactc gtaatcgaaa tctgacactg ccctcactcc tctaattatc
     2221 gccaccgctt ttaactcttt ggttaggtcc gccaaaagcc cggaaaatgg cactacctgt
     2281 actcgacgta gatttccaac tgaagcctta accatctcca atctttcttt cttggagaaa
     2341 agtggcttct tggttggatt ttcagcaatg gcgatggtga gttcgtcaaa gatattcaga
     2401 gctcttttta tcaaatcgag atgtccattg gtcatagggt caaatgtgcc gggatagaca
     2461 gctcttctca tattttcctc cgagtgttat cttttcttgg ataatatgct aactatggta
     2521 tctccaaatt ttcttcgcct cagctcaacc aaatcagaag aatccaaatt cagctcctct
     2581 tttttatgat gctctattat caccaaagca ttaacgccta aaatacctgt ttctttcaga
     2641 taatctataa ctccttgagc ataacccaaa agataaggtg ggtcagcaaa gactatctca
     2701 aattttcttc cttctcgagc caatttttga agtgcttttc tataatccag tttcaatatc
     2761 tcggcacaag aaagacactc caaatctccc aaatttcttt ggatgatctt ggtgctttgg
     2821 gctgagaaat caacgaaagt aattgaaata gcacctctgg agatggcttc tatgcccaaa
     2881 tttccggttc cggcgaaaag gtccaggact ctttttcctt ccagctctcc tttgagtatg
     2941 ttgaaaatcg attccttcac cctatctgct gttggtctaa agttcgcctt ggaggaaacg
     3001 aataatctcc tgcttttcaa ttttccacca actattctca agaactccaa ctcctattta
     3061 acttcaacgt aacctcttat cttctctagg gtcttttccc caattccgtt gactttcaaa
     3121 agatcatcta cctgtttgaa ttttccattt ttctctcggt attcgataat cttcttgctt
     3181 aaggtcggac caatataagg caagagatca agttccgccc agctggttgt attcaaatcc
     3241 accttttccg tgggagcagg tgaggctatt ttagactgcg tatgttgaat ggtcttggtt
     3301 gttttttctt gtccccgttc tgccttagat tccctgaaga ttatctccat cggaaaattg
     3361 gcgtggtatt ttttatatat ggcaattccg ctgccaacca gcaaaagaac gagaagaact
     3421 aacaatcctc tcatttcagc caaattgaaa tcccaccatt tcatgttagc cttaatgata
     3481 tagaacctct attaatagtt tgccaatttt ctctaaactt tctgaactgc atttgtccgg
     3541 tgtgtcggaa agggtgtgcc agtaagaata gtcgaaatca ataacatcaa tacagggtac
     3601 tcctgcgctg atcaatggaa ggtgatcatc tataagtgta tgtttgactg agctaccaaa
     3661 acattggtag ttgagttttc tggctctgga ccagaccaaa tccactactt tcggtgcata
     3721 agcgacagaa tatccttctg gatagatatt caagttttta tcccctatca tatctaaaat
     3781 aatgccaaat tggggacgat aatctcccaa gttctttgca aaatacttcg agcccagaca
     3841 aaagtcatca cttccctctt ttcccatatc ctccccatca aggaggacaa tatcgactcc
     3901 gtacttgggc ttggatttgg atagaacgtt tgccatctcc aagagcactg ccacgcccga
     3961 agcaccatca ttcgcccccg gaattggctt atctcttaaa ctggagtcaa gttctttatc
     4021 cgcagaaggg cgggtgtccc aatgcgcgcc gagcaaaacc cttttggtct cctctggata
     4081 aaaggaggca atgatattgg tcaaattcaa atccgaattg ttcagggaat cgtgatagac
     4141 gaaattctgc attcttacta aattagtgcc ttggcttagc ttgtcaagca aatagttctt
     4201 ggttttcgaa tggttgatgg agcccggatt tcttggacca aattcacact gttccaataa
     4261 atattggaaa gccctatccc tgtcaaaaga cggaaggtgg actgacgaac agcatagaaa
     4321 aaaggtcaaa agaaaaaggg ttattctctt agtgtttagc ataagattag aaccttatct
     4381 caaaccaaat tcccagctgc cgggtatggg attttatctt ggttatcttg tcgttgctat
     4441 ccatccatgt cccggacaaa cccgcctgaa cttgcgaaga cagattatat cctgccctgg
     4501 ggcttatgtt aagctctgtc ctatgctgta tgaggttgga tggattatct tcggttgatt
     4561 tcttttgttt atgcgtccta taactaatat ccaaagacag gctcaggttg ctctcaaatt
     4621 ttatcttctt gagcaagggg aatttaattc cctgcggtgc tctgaaggaa tagctgttat
     4681 ttagtataac ggtttggtcg gagtcaactg tagttgtgcg gtttccaccc tgaaaaccta
     4741 aatcctcatt tttgttttgg cttttatcaa tccccaaatt ggtcctgatt ccgttcttcc
     4801 ataccaggga gaaggaggct aagggattga atgagattaa agtgtttttg ctttttgttg
     4861 cgtcaactaa cttatccttg gtggttccaa ctttcctgga atatccaaac ctgaatccca
     4921 gactgctaac gtatttattg aggaatttga acttattaat attgttccaa ccaaaagata
     4981 actccggaaa ggtcttactc ctgttttcag tatgggcgtt ggggttgcga gtatctgtaa
     5041 ttgtttcaga gtaacttata tcgaggttgg tactaaggat agctatgcca cttctcaagc
     5101 tgtaattatc tcttgtggtc agggcatcgt tgatcgttcc tgaggtggta ggtttcattc
     5161 ccacgccagg gtcatctgtg aaaccgagct gatatctaaa ggagggtcta tccaaaagac
     5221 cacttcgggt aaagctcttg tttctgctga ctgaaccctg taaggggttg attctcttgg
     5281 acaaaaaacc gatccttttt cttatccaac tcaatggacc gcttttagtt tctttcttat
     5341 cagctgatgt gcttttcggc ttgtcttcca aagacacctt ttccaagagc ttctgccatc
     5401 tgagagtgaa gctagctgat ttcgtgttgt catttgtaac cgacctggta ccatccgtaa
     5461 actgcagggg gtcacaattt tcgttgtagt tggagttgaa cgaaaaacga tggtccaaga
     5521 aggagaagat cctaggcgta tagctggcgg tgtaactttt tctgttggtt ctctcaattc
     5581 ccaatctcat cttgctggga ttgagggaaa attttatctg gttaccatct ctgatatccc
     5641 gattggtcct ccaggtatag ccaagaggta agttgctaaa gggagaaaaa gccaagttca
     5701 tcccgccatc gaagtctcta gtatataccg aagtggggct tccgaaatca tttaatgaat
     5761 acgatttcaa tctattcacc cttccatcga aattcaaagc ggtgggaaga taatagaatt
     5821 tcgtttggga caacttattc aacagaaaaa ccttcttgct ccaggttaaa gggctaaagg
     5881 aaagcttact tggcggagca aggtcgtacc ccccagtaac ctgataatta gacgaattgg
     5941 aaaccggagt agccggagac ctaccttttt gcttactata tgacatcctg gtggagatgc
     6001 gattcaaagt ccagttagta atccagtttt ttgtattctt cttaaaactg ggactaaaac
     6061 taaaagcctc gttaactgat tcggttttct cctggtctct ctgttcaact ggaagcacga
     6121 tatcagagcc tactttcagc ctcggaaccg atagattctt agaccagcta taggaaaagg
     6181 gcaagtcaag tccccaactc ggcggtagaa acttctctaa gtttatgctg gtgccgaaag
     6241 atgttccggt actattcacc cccgacccgc gatttgttct gaggtcatga aactcgctat
     6301 ctgttttgtt taaactgaga ctgacgctta ataagtcagc gaaattaaaa gacgcggaac
     6361 ttcttattgc ccatcccgga ttctttctaa catctgccac tcttaactca tctacccaaa
     6421 cctcacccga ttttacttct gcggataaag tatcgttctc cacacccata ctgaaccagt
     6481 cgacttgcga aagagtgggt tttcctctta tcctgtagtg ttcctgggtg atatcatacc
     6541 cttctgaatc tggaggctct tccagcatat aactctttat tctggttacc tcttcgaagt
     6601 tgatcgcaac gtaattgcgc tcatcccacc cgggataaag attagtatga tattcgtaat
     6661 aattgactga atcagttcct attctaaaga agaattcaag tccagcagta tcactcggag
     6721 catgaatatc tggaccatga acgtacatct ttatgtcttt ataattggtg taatcttcta
     6781 cttggtaaag agttcgatat gccgctccat aatatccggg ctttaagttc tcgtatttta
     6841 agaccaggga ttgttccttt tgtctcactc cggtgttctt atcctcctct ccggctaccc
     6901 ccggaggaga actatattct ggattctcgt gagtgtttat tacaaagatt ttaaagcttc
     6961 ctccgccctc aactggtaaa gagtcaattg gagaagtccc tgaactcttc catctattac
     7021 ctaccagctg gaatgaggct atccggattg aatcacaatc ctccacccag accctggcga
     7081 atctgatatc gctccagcca ggagccccta ctgtatcata attaccttct cccttaagag
     7141 gaattctgta gagtttccag ttttgtcctg aaacctcaac cggagcatca cttaccaaat
     7201 caatgctgaa ttcaaaataa ttattactca catctggaac atcattacgg tttaagtcct
     7261 cggtatcagg atatcttcct ctgtccggat cactggcatt tccctgagtg ccatttatct
     7321 gactgtaatt attcttgtca acgttcgggt cgtaggacca attgtcacca ctcggatcac
     7381 tcaaggacgt atcatatttt gacctctcct catcatccca ccaatggtcc agccctttat
     7441 cctcatcatc ttgcaggatt ccatctttga aaccatttct ggttgaatcc tcagtatcta
     7501 agacgccatt attatcaata tcttcattca cctgacctag gtctatatga atcttgcctt
     7561 tatcgccgtt cagccaaatt tccaaaaatt cggtcttgct ttggtcgtaa ctccctttgg
     7621 gtagtgctcg cattattcca ttccacacat tgacatttgg tacaaaagaa gtatcagctg
     7681 ggatatgcgg ggaggaacta tcgagtttca aaaccaagat atgggtcttt tggtcttcag
     7741 ccgtggtttc cattgcgggc cagatatcct tgacataata gccatcataa ggattatacc
     7801 aaatcattct tcctctttga atgaggtctc ttccagttgg catactggag agagtccatg
     7861 tccctctgcg aatccccaag tcagtatatt ccagggaacc ctcgaaatcg tctatgaacg
     7921 catcgttctt gatattggga ttgggtaagc tctgggcaat ttcagccgag aatgttaagt
     7981 ttgaaggggc atcagtttca actaaaggga gagcatcggc cagtttagtc attaccaaag
     8041 gagatgagtt gaattggaga ttgccatccc aaatgaaatt ccttgttggc tcctgcccaa
     8101 ctctgggtct gtcttcacct gttttctcgc ttttatacaa agccgaaagc cccattttca
     8161 aatttcccat atcgtagttg gtgtttagtc caaaaagagt ctttttctct aacataaaca
     8221 ggggcgcata ttcataatca atggttagat tagcagttgg atccaagacc ttgtcgttgg
     8281 tgaaagttat ctgccctatc tcatagatta tgttgtaatc aacgccgcga gtcagacgct
     8341 ctccattcag ggtaacgatt tccgagcctt cgattatgct ggtccgacct aaagagtact
     8401 gggtgctgcg gctggcgctt ttggtataaa tgtagtattg ggaagcatcc atcctatcgt
     8461 tgttgttact gttatcgtat atcgacggga cctttacgct tagaccctca taggcaaaag
     8521 gtttcttcat cggaaaaatc agtaaacctt catggaaatt aatttttgta tcgactatat
     8581 caactgacct atccgggatg ggttcacctt tcaggttcag actatccaaa cccagtatgg
     8641 taatgtatgc accgctggaa tcgtaatctt tatcagcttc tatatctgtc ccacttccct
     8701 tataaattct tagctcgaaa ccttccttat cgatatctct tcctcttaaa tcatacacgt
     8761 ttttccattc atacgcccag gtgataaatg agggcttggg atattcggct ttaatcaact
     8821 tcaatgtgtc ggtgatatca ttgcctatct tggttgcaag atgccctact tctattatcg
     8881 agccgggata gccggctggt atcttcttgt aacgaatagc gacaatggct gcttcctcgc
     8941 gacggggatt gaatttgatc cagaaattgg tactatcttg agttatgccc cagcggtaag
     9001 tggagtcagg aactaaatga aatcttctgt tataggcctc acctgggtat tcacttgtat
     9061 cccgggggtt cacgcaagca tacccaaaca gatcagtacc tccagaacct gtggtattct
     9121 cgaaaaccac caaagaggta tcaatgattg aatccccttt attgaaatca tttaccgtat
     9181 gactcgtatc caggtccttg gagtgaccta aatagaaata agtccagcgc agaaattcgt
     9241 aatcccggat aaactgcgga gttacctggg cacctgcctg gaagattgct ttttcagttg
     9301 agcctttttc ctggcttgcc accacagtca tatccagccc accaacctgt gctgtagcct
     9361 ttgctccaaa aagccctttt atggttccgc tataacctgc caaacccgga atcgaccagc
     9421 tggtattgcc taactcaatg cttttgataa cctcgtcctc ttccccgtta tatctaagct
     9481 ggatattgtt ctccaaatcg gtcatcctct tgctgtcctg gtcaaccttg actgtaatct
     9541 tactgccgat tgttccggtg atggtgaatc tggagtcctg ttccatttct aaagagggga
     9601 acttggattg tcgggaagta gcggtattgg tgaagccatc ctgccactgg ctacgaccag
     9661 caaagcttat tcgcttatat ccgttcactt tcaatcctgg accaccttca cctactatct
     9721 tggatatggc tttagggaac ttgaccggta tttcaaatcc cagaaggtct tttttgccac
     9781 cttgttcact gacctccatc gttttccctt gttcaccaag ccaaagtttc tgggtttctg
     9841 aagcgctaac ctgtgaaaca tagtctggca aatccaagac caaatcttga ccgatttcgt
     9901 aattacccgc tttggtggat aattccaccc agcccaaatc ataatcaatc ttaacttcct
     9961 tttggagttg cagttttctt tcgagaaggg gggcagtggg aaaagccata gaaagacctt
    10021 tcttttgggc taaatcggac cgataaaaag tctcggggta agatagattc agattgaagt
    10081 ctaaaccttg ggcgtaaaca ggtgtgggaa tgtgaaacaa caaaaccgcc aagctcaaaa
    10141 caaggagaac cattttggcg gtgactggct tctgctccga agaatacata actctacctc
    10201 ataaattaga aagcattaga tatattctat attaacctcc tttttgagaa gtttcaagtt
    10261 accccaaaaa gacgacctct tcctccaatt ttatcttgaa cctttccttg actcttcttt
    10321 ttaatatatc ggctaaagat ttcacatctt ttgctctggc tttgcctaaa ttaaccacta
    10381 tgttggcgtg cttatcataa atagcagcgt cgcctacaca taaattctta gcgccaacct
//
LOCUS       DASWLN010000134        12003 bp    DNA     linear   ENV 20-FEB-2024
DEFINITION  MAG TPA_asm: Candidatus Zixiibacteriota bacterium isolate
            SMAG_U2949 k141_744678, whole genome shotgun sequence.
ACCESSION   DASWLN010000134 DASWLN010000000
VERSION     DASWLN010000134.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37960594
            Sequence Read Archive: SRR10376091
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Candidatus Zixiibacteriota bacterium (soil metagenome)
  ORGANISM  Candidatus Zixiibacteriota bacterium
            Bacteria.
REFERENCE   1  (bases 1 to 12003)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 12003)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (26-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/12/2024 08:28:26
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 1,440
            CDSs (total)                      :: 1,416
            Genes (coding)                    :: 1,412
            CDSs (with protein)               :: 1,412
            Genes (RNA)                       :: 24
            rRNAs                             :: 1 (16S)
            partial rRNAs                     :: 1 (16S)
            tRNAs                             :: 21
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 4
            CDSs (without protein)            :: 4
            Pseudo Genes (ambiguous residues) :: 0 of 4
            Pseudo Genes (frameshifted)       :: 0 of 4
            Pseudo Genes (incomplete)         :: 3 of 4
            Pseudo Genes (internal stop)      :: 1 of 4
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..12003
                     /organism="Candidatus Zixiibacteriota bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_744678"
                     /isolate="SMAG_U2949"
                     /isolation_source="soil"
                     /db_xref="taxon:2053527"
                     /environmental_sample
                     /country="USA: Oregon"
                     /lat_lon="42.99 N 123.63 W"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            <1..261
                     /locus_tag="VGB16_04475"
     CDS             <1..261
                     /locus_tag="VGB16_04475"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEX9916973.1"
                     /translation="EAKGILKQIFVMETAYRQEYNVYHANKDTIGVVIPSNARYAYNI
                     GTADSTHFIASAGADLDDDATRDSMTIDTSGVIIQRSNDAAD"
     gene            361..1350
                     /locus_tag="VGB16_04480"
     CDS             361..1350
                     /locus_tag="VGB16_04480"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_017211321.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="SDR family NAD(P)-dependent oxidoreductase"
                     /protein_id="HEX9916974.1"
                     /translation="MLMKNQKVLVTGAGGFVGSHLVEDLVEVGAKVRAFVHYNSRNDW
                     GMLEQLDKRILREIEVVAGDIRDADTVRKIVNGYEVVYHLAALIGIPYSYHSPQDVVD
                     TNIGGTLNILLAALDSDVERVIHTSTSEVYGTAKYVPMDEKHPLNPQSPYAASKVGAD
                     QLALSFYRSFGLPVGIIRPFNIYGPRQSARAVIPAIIIQALKGNRIHIGSSYPTRDLT
                     FVKDSSSGFVSFAKSKKTIGEVVNLGSGYEISVEKIVDVIEKITNKKLKVVIDKKRIR
                     PKKSEVDRLFSNSKKAEKLFDWKPKTRLEDGLRETILWIEKNIGKYKEKIYNI"
     gene            1445..2149
                     /locus_tag="VGB16_04485"
     CDS             1445..2149
                     /locus_tag="VGB16_04485"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011499632.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016779 - nucleotidyltransferase activity
                     [Evidence IEA];
                     GO_process: GO:0009058 - biosynthetic process [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="sugar phosphate nucleotidyltransferase"
                     /protein_id="HEX9916975.1"
                     /translation="MKVIVLAGGQGRRLAPYTIVLPKPLMPVCDAPILEIIIRQLKSY
                     GLTDIVLAIGHLGNLIQTFFGNGKKFGVKITYNLEDKPLGTVGPLPLIDSLSKTFMVM
                     NGDLLTTLNYDNLIEYHHKNKAIATIAVREAEVNINYGVIKSKGNELCDYIEKPILEY
                     MVSMGIYVFEPEVLKYIPKGKKFDFPELMQLLLNEKKKVGLYKSKDFWLDIGRHEDYQ
                     KAIEEYDKIKEGLFKK"
     gene            2146..3291
                     /locus_tag="VGB16_04490"
     CDS             2146..3291
                     /locus_tag="VGB16_04490"
                     /EC_number="2.6.1.-"
                     /inference="COORDINATES: protein motif:HMM:NF013227.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008483 - transaminase activity [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="DegT/DnrJ/EryC1/StrS family aminotransferase"
                     /protein_id="HEX9916976.1"
                     /translation="MKWKVALSDIDLDKRETDAVKKVLKSKWLSMGDRVAEFEKGFAE
                     YLKVKYAIATNSGTAALHLALKCLNTKPGDEVLVPALTFVASVNAILYCQAKPVFVDI
                     TSLDDFNLSPEDLEKKITKKTKGVLVVHYAGFLADMDKITMIAKKHKLFIVEDSAHAI
                     GTGWRDKMAGAIGDVGCFSFFSNKNMITGEGGMLVTDSLRIAKKAKIMRSHGMTSLSW
                     DRYRGHASSYDVTELGYNYRTNEISASIGIEQLKKLSENNKKRKELTRLYIGNLKEVD
                     GLCIPFLSFPRESCYHLFPILLCPEINRAKFMQKLQSRGIQTSIHYPLVTEFSFYREL
                     FKDSLIDLPKSKYVSKHQVTLPLHPLLKPKNINYVCSQVKKAIKELA"
     gene            3291..4262
                     /locus_tag="VGB16_04495"
     CDS             3291..4262
                     /locus_tag="VGB16_04495"
                     /EC_number="2.3.1.-"
                     /inference="COORDINATES: protein motif:HMM:NF024871.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008080 - N-acetyltransferase activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="GNAT family N-acetyltransferase"
                     /protein_id="HEX9916977.1"
                     /translation="MNLILIENPGARWDEFISKYSHLVFHTSIWAQILKISYDAKLAY
                     YVLEDKGEWLLAIPGMLTGRFLKLWYSLISYGGFIGKREYISTFLEFITKDIRKYKID
                     RLEIIDPEVGQSSEISGFSGNESYRHILNLENKTEEDLWKNYKPNLRRTIGEALDAKL
                     SIEKIRDSKEVELFYNLYLESIRRQRGVVRYPLGLFRGIFSLIQQGKGDLFFAKHEGI
                     TTAGIVIIYSEDIAHYFHGGSLSQYLNLRPNDLLFHAAIKEAVRKGKKQFDFMGSAKN
                     LTGLIQFKEKWGTERKSLYALHQDMNRLKSWLHEIAAPYFRFRTGRY"
     gene            4292..5086
                     /locus_tag="VGB16_04500"
     CDS             4292..5086
                     /locus_tag="VGB16_04500"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011938695.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="prepilin peptidase"
                     /protein_id="HEX9916978.1"
                     /translation="MPIYLGILAFIFGLAFGSFINVCIYRIPRKEVLVLSRSHCPKCG
                     VNIRVFDNIPLLSFIWLGGKCRNCQAKISWRYPIVELLTALVFIFSYWHFGWGWEFIS
                     KTFFLILLIIIFFIDLGHKIIPDVISLPGIGLGLGFSLLVKSITFWQSMAGFLVGGVL
                     LYLIALLGDFLFKKESMGGGDIKMAAMLGAFLGWEKFLLSFFVAVLLGALVGIITIFC
                     SSKVRESRVIPFGPFLALGAFVSIFWSKELIQLYIQTQDHIQSLLP"
     gene            5121..5261
                     /locus_tag="VGB16_04505"
     CDS             5121..5261
                     /locus_tag="VGB16_04505"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEX9916979.1"
                     /translation="MDGDEGWVFWKQAKTDQMTTGFVSRFLSILGFKAPPCGGLLYFQ
                     QN"
     gene            5532..7451
                     /locus_tag="VGB16_04510"
     CDS             5532..7451
                     /locus_tag="VGB16_04510"
                     /inference="COORDINATES: protein motif:HMM:NF014567.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ATP-binding protein"
                     /protein_id="HEX9916980.1"
                     /translation="MDKRRIIRNLFSFSYESEIKLALLFFILFLVLLNFGTVHLLSRT
                     RDILENEHKLRLLSASISVKEALDNLSSQKIRDIKGLLSDLRVRLEVDNIFILGKDGE
                     VLYSSQELGQEDLTARFYGISPSKHTLLKNNQAVTSDFYERGNKTYTACYLPLKGNEI
                     LMVEAKAEPLKIIDNAFRLDVLMRLVGLLAVAVSTIFFLKAILKPYRMIKKEAKDAKL
                     PIPSKFEGADQDIDFVVDTFQKVIVELKEKEAKLQKLYQETSQKAKDLSRYNEYILGS
                     MNSGLIICDTGGLITHFNKEAQNMLDLSEEETLNKNYSVVLVEQGRLTELIKDALQNH
                     KVYLPKEMHIARKDEGRIWVSVNSSLIRDEDNKLMGVVLLINDLSDIKNLQREVATKD
                     KMAALGEMAGGLAHQLRNSVAAILGFGKLLKKMIPLDESNQEIIDGIIKESNVTEEML
                     RKFLSFAKPQDISLEKVDLAKIIEDVLNSLEDRIKQWKVKIYKRYEKTLEPIFGDKLL
                     LKQCFQNLVQNAIEAIPQGGTIQISIRREKWNKRKYSEGVRFMEIKITDNGEGIPLEN
                     LERIFNPFFTSKEKGTGLGLSLVRKIITMHNGRIEVESQIKRGSTFKIYLPVELKCLS
                     DNSRKSDVNVSVSQM"
     gene            7601..7780
                     /locus_tag="VGB16_04515"
     CDS             7601..7780
                     /locus_tag="VGB16_04515"
                     /inference="COORDINATES: protein motif:HMM:NF012267.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="4Fe-4S binding protein"
                     /protein_id="HEX9916981.1"
                     /translation="MKITDECTACGLCLPECPNSAISEGDIYVIDAKKCNECEGVEGG
                     SRCVAVCPVECIIKA"
     gene            7850..8275
                     /locus_tag="VGB16_04520"
     CDS             7850..8275
                     /locus_tag="VGB16_04520"
                     /inference="COORDINATES: protein motif:HMM:NF014058.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="archease"
                     /protein_id="HEX9916982.1"
                     /translation="MWKYRFLEDVVIADAAFIAQGRNLEELFANCALATFEVMVDTTK
                     LVACEKREVEIENEKIDELLVDWLSELIFLKDRDKIFFRDFKINISKSGKYKLKASAF
                     GEKIDTKKHKLKSDVKAVTYHLLEVTKKKNWKARVVLDV"
     gene            8609..10054
                     /locus_tag="VGB16_04525"
     CDS             8609..10054
                     /locus_tag="VGB16_04525"
                     /EC_number="6.5.1.8"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_013249050.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008452 - RNA ligase activity [Evidence
                     IEA];
                     GO_process: GO:0006396 - RNA processing [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="RtcB family protein"
                     /protein_id="HEX9916983.1"
                     /translation="MQMKKVEDGIWEIPTSEKEGMRVPARIIASDKLIKEIDERVLNQ
                     ITNVATLPGIQRYAFCMPDGHWGYGFPIGGVAAFSLEEGIISPGGIGFDINCGVRLIR
                     TNLSYEEVKPYVNKLMDEIFRLVPAGVGSSGFIRLNNKDFKEVMTEGANWCIRRGYGW
                     QEDVERTEENGCMEGANPDKVSDKALSRGVDQLGTLGSGNHYLELQVAQTEKIFHTQA
                     AEIFGIGIPNQVFVMVHCGSRGFGHQIATDYLKTFGTALSKYGIRVNDRELACAPFKS
                     PEGQAYYQAMACAANSAFANRQLITHRIREAFTMVFKKKPEDLGMYLVYDVAHNIAKM
                     EKHKINGKDETLIVHRKGATRSFGPDRPEVPEIYRKVGQPVIAGGSMETSSYVLVGTN
                     KATELAFGSTLHGAGRTMSRTEARRKVRGTDLRKSMEEKGISVRAVSMSGLAEEAGIA
                     YKDISEVVDAVDRIGISKKVVELKPIGSIKG"
     gene            10055..10639
                     /locus_tag="VGB16_04530"
     CDS             10055..10639
                     /locus_tag="VGB16_04530"
                     /EC_number="2.1.1.-"
                     /inference="COORDINATES: protein motif:HMM:NF013742.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008757 - S-adenosylmethionine-dependent
                     methyltransferase activity [Evidence IEA];
                     GO_function: GO:1904047 - S-adenosyl-L-methionine binding
                     [Evidence IEA];
                     GO_process: GO:0032259 - methylation [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="O-methyltransferase"
                     /protein_id="HEX9916984.1"
                     /translation="MRKDIERFLKRLEKKNEKEKKAGLPIQKRMRSIPPEAGLFLYLL
                     VKISKAQRILEIGTSQGYSTIWLAQAAEETGGKVTTLEINPTSAAKAEKHLKQVKLDK
                     FVKIKVGDAERILANMREKFDFVFIDAEKKDYLTYFSLVCSKINRNGLIVADNVISHA
                     EVLADYRNSVQRRPNFQSLIVPIGSGEMLSYKTK"
     gene            complement(11109..11768)
                     /locus_tag="VGB16_04535"
     CDS             complement(11109..11768)
                     /locus_tag="VGB16_04535"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_007418773.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003677 - DNA binding [Evidence IEA];
                     GO_process: GO:0000160 - phosphorelay signal transduction
                     system [Evidence IEA];
                     GO_process: GO:0006355 - regulation of DNA-templated
                     transcription [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="response regulator transcription factor"
                     /protein_id="HEX9916985.1"
                     /translation="MKRIRVLIADDHKIVRQGIRKLLEAEPDLEVVGEALDGREVVKK
                     ASQLKPDLVLIDIAMPNLNGLEATRQIKKALPKVKVLILTMHKNEEYVLQSFQAGASG
                     YLLKDAAVEDLVFAIRSVHRLDSFLSPAVSKTVIGAYLSKNKSKKKLSAFEVLTDRER
                     EILQLIAEGHTNQQVADTLFISVKTVEAHRSNIMRKLDIHDITELVKYAIKKGIIDLN
                     T"
     gene            complement(11737..>12003)
                     /locus_tag="VGB16_04540"
     CDS             complement(11737..>12003)
                     /locus_tag="VGB16_04540"
                     /inference="COORDINATES: protein motif:HMM:NF014567.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ATP-binding protein"
                     /protein_id="HEX9916986.1"
                     /translation="KAKDVWVGLSRKESNVHMTIRDNGIGFDVGKLYAEDYAKKGFGL
                     MSMRERVQLLNGSFNIRSKKNYGTKLIITISLAQDEKNKSSHSR"
BASE COUNT         3690 a         2131 c         2901 g         3281 t
ORIGIN      
        1 gaggcaaaag gaatcttgaa gcaaatcttc gttatggaga ccgcttatcg tcaagagtat
       61 aatgtgtatc atgctaacaa ggacacgata ggtgtggtga taccatctaa cgccaggtat
      121 gcgtataaca tcgggacagc cgatagtaca cactttatag cttcggcagg tgccgatctg
      181 gatgacgacg ctaccagaga tagcatgact atagatactt caggagtaat tatccaacgg
      241 tcaaatgatg cggctgatta attattgact tgaatttgtt atctaaagga gaaaggggat
      301 cttaaaaagg tccccttttt tgttgttgtt ttgtttccgt caattatata tatttataat
      361 atgcttatga aaaatcaaaa agtattggtt acgggtgcgg gtgggtttgt gggaagccat
      421 ttagttgagg atttggtgga ggttggagcc aaagtgaggg cttttgtgca ttacaattcc
      481 cgcaacgatt gggggatgtt ggaacaatta gataaaagaa ttctaaggga gatagaggtc
      541 gtagcaggtg atataagaga tgccgacaca gtcagaaaga tagtcaatgg atacgaagta
      601 gtttatcatt tagctgcttt gattggaata ccctattcct atcacagccc tcaagacgta
      661 gtagatacta acataggtgg aaccttgaat attctattgg cggctttgga ttcagatgtg
      721 gaaagggtga tccacacctc gaccagtgaa gtttacggca cagccaagta tgttccaatg
      781 gatgagaaac atcccctgaa ccctcaatcc ccgtatgcag ccagcaaagt cggagctgac
      841 cagttagctt tgagttttta tcgctctttc ggtttgccag ttgggataat ccgtcctttt
      901 aatatctatg gccccaggca atcagccaga gcggtcatcc ccgctataat tatccaagcc
      961 ctcaaaggaa acagaatcca catagggtca agttatccca ccagagattt gacttttgtt
     1021 aaagattcgt cctcgggctt tgtgtctttc gctaaatcca aaaagacaat cggagaggtt
     1081 gtaaatttgg gctcagggta tgaaatatcc gtagagaaga tagtagatgt aattgagaaa
     1141 atcacaaaca aaaagctgaa agtagtgatt gataagaaaa gaataagacc caagaaaagt
     1201 gaagtggata ggttgttttc taattccaag aaagctgaaa agctttttga ttggaagccg
     1261 aaaaccaggc tcgaggacgg tttaagagaa actattttgt ggatagagaa gaacataggc
     1321 aaatacaaag agaagattta taatatttga gttggggttg aataaagctg gagtgtcagg
     1381 cttaagcctg acttcataag atctgcctaa aggcagatac tccaaaagta gggaagaatt
     1441 caatatgaaa gtaattgttt tagcaggtgg acaggggagg agattagccc cttatacgat
     1501 tgtgttaccc aagccgctta tgccggtgtg cgatgccccg attttggaga taatcatccg
     1561 gcaattaaag agctatggtc tgactgatat agttttagcc attggccatt tgggaaattt
     1621 gattcagaca ttcttcggaa atggtaagaa gtttggggtt aaaattactt acaatcttga
     1681 agataaaccc ttaggcacag tgggcccgct tcctttgata gatagtttga gtaagacctt
     1741 tatggtgatg aacggtgacc tattgaccac tttaaattac gacaacttga ttgaatatca
     1801 tcacaaaaac aaagccattg ccaccatagc agttcgagag gcagaagtga atatcaatta
     1861 tggagtaata aaatcaaaag gaaatgagtt atgcgattac attgaaaagc caattttgga
     1921 atatatggtc agcatgggaa tttacgtttt tgagccggaa gtattaaaat acattcctaa
     1981 aggaaaaaag ttcgattttc ctgagctgat gcaacttctc cttaatgaaa aaaagaaagt
     2041 gggcttgtac aaatccaaag atttttggct ggacataggg aggcatgagg attatcaaaa
     2101 agcgattgaa gagtatgaca aaataaaaga ggggttgttc aagaaatgaa atggaaagta
     2161 gcactctcag atatagattt ggataaaaga gagacggatg cggttaaaaa agtattgaaa
     2221 agcaaatggc taagcatggg ggatagggta gctgaattcg aaaagggatt tgcagaatat
     2281 ctgaaagtaa aatatgcaat cgccaccaat agtggaacag ccgcactgca tctggctttg
     2341 aaatgcctaa atacaaagcc gggagacgag gttttggtcc cggctttaac ttttgtggca
     2401 tcggtgaatg ccattttgta ttgtcaagcc aagccggttt tcgttgacat aaccagctta
     2461 gatgatttca acctctctcc tgaagattta gaaaaaaaga taactaaaaa gaccaagggg
     2521 gttttggtag tgcattatgc aggttttctg gcagatatgg ataagataac tatgatagcc
     2581 aaaaagcata aactgtttat agtggaagat tccgcccatg cgattggaac tggatggaga
     2641 gacaaaatgg ctggggcaat aggagatgtc ggatgtttca gctttttctc caacaagaac
     2701 atgattaccg gggaaggcgg aatgttagtt accgatagct tgagaatagc caagaaagct
     2761 aaaattatgc gttctcacgg aatgaccagc ttaagttggg ataggtatcg tggccatgcc
     2821 agttcttatg atgtgacgga gctgggttac aattacagaa ctaacgagat aagcgcaagc
     2881 ataggaatcg agcagttgaa aaagctcagc gaaaacaata agaaaagaaa agagctaacc
     2941 cgactgtata ttggaaatct aaaggaagtg gacggcttat gtattccctt tttgagtttt
     3001 cctcgcgagt cttgttatca cctctttcct atccttcttt gccctgagat caacagagct
     3061 aagttcatgc agaagcttca gtcgagaggg attcaaacca gcattcatta tcctttagtt
     3121 actgagtttt ctttctatag agagttattc aaagacagtt taatagatct acccaaaagc
     3181 aagtatgtaa gcaaacacca agtcacctta cctcttcatc ccttactgaa accaaagaat
     3241 ataaattacg tttgctccca ggtaaagaaa gccataaaag agctggcctg atgaacttga
     3301 tcctgataga aaatcctggc gcaagatggg atgaatttat ttccaaatat tcgcatctcg
     3361 tctttcacac ttcaatttgg gcccaaatct taaaaattag ctacgatgca aaattagctt
     3421 actatgtttt agaggataaa ggggaatggc ttttagctat tcccgggatg ctgactggta
     3481 gattcttgaa attgtggtat tcgcttatat cttacggcgg gtttattggc aaaagggaat
     3541 atatctccac gtttttggag tttatcacaa aggacataag aaagtacaaa atcgaccgat
     3601 tagagataat agaccccgag gtgggtcaat ccagtgaaat ttccggattt tcgggcaatg
     3661 agtcatatcg gcatattttg aacttggaga acaaaaccga agaagaccta tggaaaaact
     3721 acaaacccaa tttgaggagg accatcgggg aggctttgga tgcgaagtta tccatagaga
     3781 aaataagaga ctccaaagag gtggagcttt tttacaatct ttatttagag tcaatccgca
     3841 gacaacgggg agtggtgcgt tatccattgg ggcttttcag aggtatattt tctctaatac
     3901 agcaagggaa aggagatctg ttttttgcca aacatgaagg tataaccacg gctggaatag
     3961 tcataatcta ttctgaggat atagcccact attttcatgg cggatctttg agtcaatatt
     4021 tgaatctcag accaaatgac ttactttttc atgctgctat taaagaagcg gtgcgtaaag
     4081 gaaagaaaca atttgatttt atgggctcag ctaaaaactt gactggtttg attcagttca
     4141 aggaaaaatg gggcactgag aggaagagtt tatacgcact gcaccaggat atgaacagat
     4201 tgaaatcttg gttgcacgag atagccgcac catatttcag atttcgcaca ggcagatatt
     4261 gaagttattg tagaacgagg aattaagaga tatgcccatt tatttaggaa ttcttgcttt
     4321 tatctttggt ttggcttttg gtagttttat aaacgtctgc atttatcgta tcccgaggaa
     4381 agaagttctg gttctctcta ggtcccattg cccgaaatgt ggagtgaata taagagtatt
     4441 tgataatatt cccctgttga gttttatatg gcttggcggc aaatgtcgaa attgtcaggc
     4501 gaagatctcc tggcgatatc cgatagttga gctacttacc gcactggttt tcatattcag
     4561 ctattggcat tttggatggg gatgggagtt catatccaaa acattttttt tgattctgtt
     4621 aatcataatc ttttttatcg acttggggca taagataatt ccggatgtca tctccctgcc
     4681 gggaataggt ttagggttgg gtttttctct gttggtaaaa tcgattacat tttggcaatc
     4741 tatggctgga ttcctggttg gcggagtgct tctgtatttg atagctctcc tgggagattt
     4801 tcttttcaag aaggaaagca tgggaggagg agatataaaa atggcggcta tgttgggagc
     4861 ttttttaggt tgggagaagt tccttctttc cttttttgtg gcagtgcttt taggcgcttt
     4921 ggtaggcatt atcaccatct tttgttcctc caaagtgcgt gagagcagag tgatcccctt
     4981 tggtccgttt ttagcactgg gcgccttcgt atctatcttt tggagcaaag agcttattca
     5041 actttatatc caaactcaag accatattca gtctcttctt ccttagttta ttaagatcgc
     5101 acctgaaaac cctcgtcccc atggacggcg atgaaggatg ggtcttctgg aagcaggcaa
     5161 aaacagacca gatgacaaca gggtttgttt cccgcttttt gtcaattttg ggttttaaag
     5221 ccccgccctg tggtgggctt ctttacttcc agcagaatta atcccttata caaaattgcc
     5281 gataaagata ataatgaagc ccgcatcagg gcttgtatga gtcattactc gcccctgcca
     5341 gaaatggcga ggtgagcttc ggtcgcctct cggcgaacct cgcttaaggc gggcgaagtt
     5401 tcgctcgcct ctcggcgctc gcctctcggc gaagccgagg gaagccgagg gaagccgagg
     5461 gccttttgtc ccgccacagg cgggatttat atataggaag ccccgtgatt tatcacgggg
     5521 aggcttcact tatggataaa agaagaatca tcagaaacct gttctccttc agttacgagt
     5581 cagagatcaa attagcccta ctcttcttta tactcttttt ggtattgctt aatttcggaa
     5641 cggtgcatct gctttcacga accagagaca ttcttgaaaa tgagcacaaa ctcagattgc
     5701 tttcagcttc aatctctgtc aaggaagccc tggataatct ctcatcccaa aaaataagag
     5761 acataaaagg tttgctttcg gatttgagag tcagactcga agtggacaac atcttcatat
     5821 tgggaaaaga tggagaggtt ctttactcat ctcaggagtt aggccaggag gatttaaccg
     5881 cgaggttcta tggaattagc ccttccaagc acacactatt gaaaaacaat caagcggtaa
     5941 cttcggattt ttatgagcgc ggaaataaaa cctacacagc ctgttatcta cccctgaagg
     6001 ggaatgagat actgatggtg gaagcaaagg ctgaaccact gaagattata gataacgctt
     6061 tcaggttgga cgtgttgatg agacttgtcg gacttttggc tgtggcggta agcactatat
     6121 tctttttgaa agccatcttg aaaccttacc gcatgattaa gaaggaagcc aaagatgcca
     6181 aattacccat accctcaaag tttgagggag ctgaccagga catagatttt gtagttgata
     6241 cttttcagaa ggtgatcgtt gagttgaagg agaaggaagc gaaactgcaa aaactgtatc
     6301 aggaaacaag ccagaaggca aaagacctga gccgctacaa tgaatatata ctggggagta
     6361 tgaacagtgg actcatcata tgtgacacgg gaggacttat tactcatttc aataaagaag
     6421 cacaaaacat gttggacttg tccgaagagg agacgttaaa taagaattac tctgtggttt
     6481 tagtagaaca aggccgcttg actgaattga tcaaggatgc actacagaat cataaagtct
     6541 atctccctaa agaaatgcat atagccagaa aagatgaggg aaggatatgg gtgagcgtaa
     6601 acagttccct gattagagac gaagacaaca aactgatggg cgtggtgctg ctgattaacg
     6661 atttgagtga tataaagaac ctgcagcgag aagttgcgac caaagacaag atggcagctt
     6721 tgggggaaat ggcaggaggt ttggctcatc aattgagaaa ttccgtggca gctatcttag
     6781 gatttggtaa actccttaaa aagatgatac cgttggatga gtccaatcaa gagataattg
     6841 atggtattat aaaggagtcg aatgtaactg aagagatgtt aaggaaattt ctgagcttcg
     6901 ccaaaccgca agatattagc ttggagaaag tagatttagc aaaaatcata gaggatgtgt
     6961 taaacagctt ggaagataga atcaagcaat ggaaagtgaa gatatataaa agatatgaga
     7021 aaacattaga gccgattttt ggagataagc ttcttttgaa acaatgtttt caaaacttag
     7081 ttcaaaacgc catagaagcc ataccccaag gaggaactat tcaaatcagc attcgacgag
     7141 aaaaatggaa taagaggaag tattccgaag gggtgagatt tatggagata aaaataactg
     7201 acaatggaga ggggataccg ctggagaatt tggaaaggat atttaacccg ttcttcacct
     7261 ccaaggaaaa ggggactggt ttaggtttaa gcttggttag aaaaataata actatgcata
     7321 atggtcgcat tgaagtggaa agtcagataa aaaggggaag tacctttaag atttatttgc
     7381 ccgtggagct gaagtgtctc tcggataatt ctcgcaaaag tgatgtgaat gtaagtgttt
     7441 ctcaaatgtg aaagagttga gttttctggg gtttttggaa ttttcttcaa aaagtgattg
     7501 acaagcccat ccagaattaa atatattatg attcaaattc aatcagttca gtatggattt
     7561 tgggattaac ctttatgctt ggaggtagag tgacatggcg atgaagatca ccgatgagtg
     7621 cactgcttgt ggtttgtgct tgccagaatg tccaaattct gcgattagcg aaggggacat
     7681 atatgtaatt gacgcaaaga aatgcaatga gtgcgaaggt gtagagggtg gttctcgttg
     7741 tgtggcagtt tgtccagtgg agtgtataat caaggcttaa gaattttgtt ggtctaagat
     7801 aaatgcgccg cagaggagat ttctggtggc gcattttttg tgagagaaca tgtggaaata
     7861 cagattctta gaggatgtgg ttattgcgga tgcggctttt atagcccaag gaaggaactt
     7921 ggaggagctt tttgctaatt gcgctctggc aactttcgag gtgatggttg ataccactaa
     7981 attggtggct tgcgagaaaa gggaagtaga aattgaaaat gagaaaattg atgagctatt
     8041 ggttgattgg ttatccgaat tgatttttct taaagacaga gataagatat tttttaggga
     8101 ttttaagata aatatcagca agagtggtaa atacaaatta aaggcttctg ctttcggtga
     8161 gaagattgat acgaaaaagc acaaactgaa gtcagacgtc aaggcggtaa cttaccatct
     8221 tttggaagtg accaagaaga agaattggaa agcgagagta gtattggatg tatgaagcct
     8281 gacggacaca gaagaatggg tcggataaat ttcaaccagt agagcgcaaa attttgcgtc
     8341 tctataaatc tctgttagtg caggaattgg atttcctgca ggagatatgg ttcgggaatt
     8401 ccccggaacg ggtcttgacg acaattctcg aaccaactaa aaaaacagac cagatgctga
     8461 aacgagagat gctgaaacga gttcccttcg actttgctca gggacggtga gcgtagtcga
     8521 accgtcagta tgacaagagg gtttggtttt acggtttttg tcatcccgaa cctgtttcgg
     8581 gatctgagcc tgtataaggg gtgattctat gcagatgaaa aaggtggaag acggaatttg
     8641 ggagattccc accagcgaaa aggaaggaat gagagtccca gcacgaataa tcgcttcgga
     8701 caaactcatc aaggagattg atgaaagggt cctgaatcaa ataaccaatg tggcaacttt
     8761 accaggaatc caaaggtatg ccttctgcat gccggatgga cactggggat atggatttcc
     8821 gattggaggt gtagcagctt tttctttgga ggaaggtata atatctccag gaggaatagg
     8881 atttgacatc aattgcggag tcaggttaat tagaacaaat ctgagttacg aagaggtcaa
     8941 gccctatgtg aataagctta tggatgagat ctttagatta gttccagctg gagttgggtc
     9001 gagtggtttt attcgcctga acaataaaga tttcaaagag gttatgaccg agggggccaa
     9061 ttggtgcata agaagaggat atggttggca ggaggatgtg gaaagaacag aagaaaatgg
     9121 ttgcatggaa ggtgcaaatc cagataaagt cagcgataaa gctctctcgc ggggagttga
     9181 ccagttgggg actttgggtt caggcaatca ctatttggaa ttgcaagtag cccaaacgga
     9241 gaagatattt catactcagg cagctgaaat tttcggaata ggaataccca atcaggtttt
     9301 tgtgatggtg cattgtggct ccagaggttt tggtcatcag atcgcaacgg attacttaaa
     9361 aacctttggg acggctttgt ccaagtatgg catccgggtg aatgacagag aactggcttg
     9421 tgcgcctttc aaatcgcccg aagggcaagc gtattatcag gctatggcat gtgcggcaaa
     9481 ttcagctttc gccaataggc aattaatcac tcatcgtatc cgtgaagcct tcactatggt
     9541 cttcaaaaag aagccagagg acttaggaat gtatttggtc tatgatgttg cccacaacat
     9601 cgccaagatg gaaaaacaca agataaatgg aaaagatgaa actttgatag ttcaccgtaa
     9661 aggggcaacg cgttcgtttg gtccggatag gcccgaggtt ccagagatat accgcaaagt
     9721 gggtcagcca gtaatagctg gcggttcgat ggaaaccagc tcttatgttt tagtcgggac
     9781 caacaaagcg acggagcttg ctttcggctc aaccttgcac ggcgctggca gaacaatgtc
     9841 cagaacagag gcaagaagga aagtgcgggg gactgatttg agaaagagta tggaagaaaa
     9901 aggaatatca gtcagggctg tatctatgtc aggtttggct gaggaggcag gaattgccta
     9961 taaagatata tccgaggtcg tggatgctgt tgacagaata gggatatcca aaaaagtggt
    10021 ggagctaaaa ccaataggaa gcataaaggg ttaaatgcgc aaagacatag aaagatttct
    10081 gaagagatta gaaaagaaga atgaaaagga aaagaaagcc ggacttccaa tccagaaaag
    10141 aatgcgttct attccacctg aagccggcct ttttctttat ctattagtta agatatcaaa
    10201 agcccaaagg attttagaga ttggaacctc tcaaggttat tccactatct ggctggcgca
    10261 agccgcagaa gagacaggag gtaaagttac aactttggag ataaatccaa caagtgccgc
    10321 aaaagccgaa aaacatctaa aacaagtaaa gctggataag ttcgtcaaaa taaaggttgg
    10381 agatgccgag aggatattag ctaacatgag ggaaaagttc gattttgttt ttatcgatgc
    10441 agagaagaaa gattacctga cttattttag cttagtctgt tcgaagataa atcgaaacgg
    10501 attgattgtt gcagataatg taatatccca cgcagaagtc ttagccgact acaggaattc
    10561 ggttcaaaga cgccccaatt ttcaaagcct gattgttccc atcggaagcg gtgaaatgct
    10621 gagttataag acgaagtagg cttaactctt aaatatattc ttggatgaac taaataggcg
    10681 acccgtacgg gtcgcctatt tagttataag tgaagaaacc cgaccacagg gcggtgcttt
    10741 aaaagccaaa ccgagatcct aaaaccaagt tcgggatgac aagaatcgta aaaccaaacc
    10801 ctgttgtcat gctgacggtt cgactacgct caccgtccct gagcaaagtc gaagggaact
    10861 cgtttcagca tctctggtct gacgccagaa caggcttgtt taagcatctg gtctgttttt
    10921 cctgcataaa aagagggtgt taacctaatc tgggtagagg ggggctagga actacccaaa
    10981 tcaccaggta caccctccta aaactgacct aaatatatct ataatgcctc ttttgacaat
    11041 cagtagaaga ccctaattgt ttgtcaggtg aaatacctat atggtctttt ggggaaaatg
    11101 gagagatgtt aagtatttaa gtcgatgatc ccttttttaa tggcatattt taccaactca
    11161 gtgatatcat ggatgtccaa ttttcgcata atattagagc gatgggcttc gacggttttc
    11221 acgctgatga acagcgtatc ggctacttgt tgattagtat gcccctcagc tatcagttgc
    11281 aggatttctc tttcgcggtc agtcagtacc tcaaaggcag atagtttctt tttgctcttg
    11341 ttcttggaaa ggtaagctcc aataacagtc ttggagacag ctggactcaa gaaagaatcc
    11401 agtctatgaa ccgagcggat agcaaaaacc aaatcctcca ctgccgcatc cttgagcaaa
    11461 taacctgaag cgccagcttg gaaggactgt aacacgtatt cttcattttt gtgcatagtt
    11521 agaatcaata ccttgacttt aggcagggct ttcttgattt gccgagttgc ctccaagcca
    11581 ttgaggttag gcatagcgat atctattaag accagatcag gtttaagttg agacgccttc
    11641 ttgacaacct ctcggccatc cagcgcttca ccaaccactt ccaaatccgg ctccgcctcc
    11701 aagagctttc taatcccctg gcgcactatc ttgtggtcat ctgctatgag aactcttatt
    11761 cttttcatct tgagctaaag aaattgtaat gatcagtttg gttccatagt tttttttaga
    11821 tcttatgttg aaactcccat taagaagctg aactctttcc cgcatactca taaggccaaa
    11881 gcctttcttg gcgtagtcct cagcataaag ttttccgaca tcaaatccaa taccattatc
    11941 tctgatggtc atatggacgt tggattcctt gcgggacaaa ccaacccaga cgtctttggc
    12001 ttt
//
LOCUS       DASWLN010000135         2473 bp    DNA     linear   ENV 20-FEB-2024
DEFINITION  MAG TPA_asm: Candidatus Zixiibacteriota bacterium isolate
            SMAG_U2949 k141_618571, whole genome shotgun sequence.
ACCESSION   DASWLN010000135 DASWLN010000000
VERSION     DASWLN010000135.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37960594
            Sequence Read Archive: SRR10376091
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Candidatus Zixiibacteriota bacterium (soil metagenome)
  ORGANISM  Candidatus Zixiibacteriota bacterium
            Bacteria.
REFERENCE   1  (bases 1 to 2473)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 2473)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (26-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/12/2024 08:28:26
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 1,440
            CDSs (total)                      :: 1,416
            Genes (coding)                    :: 1,412
            CDSs (with protein)               :: 1,412
            Genes (RNA)                       :: 24
            rRNAs                             :: 1 (16S)
            partial rRNAs                     :: 1 (16S)
            tRNAs                             :: 21
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 4
            CDSs (without protein)            :: 4
            Pseudo Genes (ambiguous residues) :: 0 of 4
            Pseudo Genes (frameshifted)       :: 0 of 4
            Pseudo Genes (incomplete)         :: 3 of 4
            Pseudo Genes (internal stop)      :: 1 of 4
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2473
                     /organism="Candidatus Zixiibacteriota bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_618571"
                     /isolate="SMAG_U2949"
                     /isolation_source="soil"
                     /db_xref="taxon:2053527"
                     /environmental_sample
                     /country="USA: Oregon"
                     /lat_lon="42.99 N 123.63 W"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            <1..100
                     /locus_tag="VGB16_04545"
     CDS             <1..100
                     /locus_tag="VGB16_04545"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003517965.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=2
                     /transl_table=11
                     /product="flagellar basal body rod C-terminal
                     domain-containing protein"
                     /protein_id="HEX9916987.1"
                     /translation="TSMIIAQRGFQANARVITTSDEMLTDLVSLER"
     gene            152..382
                     /locus_tag="VGB16_04550"
     CDS             152..382
                     /locus_tag="VGB16_04550"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_018212576.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="flagellar FlbD family protein"
                     /protein_id="HEX9916988.1"
                     /translation="MIKVTRINGTDLVVNADLIEFVEATPDTIISLITGKKIVVKDTI
                     NEVIEKVASFRRFSSPQSTLIAQMKETIKEAT"
     gene            433..1110
                     /locus_tag="VGB16_04555"
     CDS             433..1110
                     /locus_tag="VGB16_04555"
                     /inference="COORDINATES: protein motif:HMM:NF015693.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0009425 - bacterial-type flagellum basal
                     body [Evidence IEA];
                     GO_process: GO:0006935 - chemotaxis [Evidence IEA];
                     GO_process: GO:0071973 - bacterial-type
                     flagellum-dependent cell motility [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="flagellar basal body-associated FliL family
                     protein"
                     /protein_id="HEX9916989.1"
                     /translation="MLEVEKAAKKEMPEGKAEKLKEKAQKGEEGKPEKGKLEEKLAEE
                     PESKPEGQSKKGGLKLKLSIPPIVKNIVIFFAAFVVLVMAAYLLTSKVLKPFMKAQIQ
                     KSATQKTKAVAKRKAQKVQTPNEVKEVFLVSDMIINPAETNGTRFLNTTIGLGVGDPL
                     TKTVLEERAPQVRDALISILTTKTIPKLVHPDGKKILRTEIMEKVNKIIAPNRILEVY
                     FVDFVLQ"
     gene            1129..2115
                     /gene="fliM"
                     /locus_tag="VGB16_04560"
     CDS             1129..2115
                     /gene="fliM"
                     /locus_tag="VGB16_04560"
                     /inference="COORDINATES: protein motif:HMM:TIGR01397.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0009288 - bacterial-type flagellum
                     [Evidence IEA];
                     GO_process: GO:0001539 - cilium or flagellum-dependent
                     cell motility [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="flagellar motor switch protein FliM"
                     /protein_id="HEX9916990.1"
                     /translation="MKTLSQDEIDVLLSAVSKGDVAPSKKKEDKKGKKSAQVYDFKHP
                     DRISKDQMRTMENMHDNLSDQLSSIYSTLQRTVVDVDLISVDQITYSEYISSLRIPSC
                     SYTISMEPLERAAIIDISPSVAFSFVDRTFGGSGRPLGVERELTGIEKSVIDKVVHRT
                     LKELKKCWEHIINLDMKLMGFESNPQFIQIVAPSETVLVISLQIKILDFSGLITICYP
                     YLTLESILPQLSGQNWVDATKKRNTEVDREINMKNIQQVAAPLVAILGRTDISIHDLT
                     RLSKGDVIRLDNSINDEIEILIGDRSKYRGKPGLCRKNRAVMITSIASQDNL"
     gene            2132..>2473
                     /locus_tag="VGB16_04565"
     CDS             2132..>2473
                     /locus_tag="VGB16_04565"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEX9916991.1"
                     /translation="MADEVKVERERVPPSEEGLAREKSKTVEPEIREQVKNGDEMVEE
                     PDEPSESDTPKESYEPPLEPKQVKTAEFQQLAKGEKLGEKQNIQMILDVTLPISIELG
                     HTAMSIGEILEL"
BASE COUNT          807 a          485 c          607 g          574 t
ORIGIN      
        1 caccagcatg attatagccc agaggggatt ccaagccaat gcgcgtgtga taaccacatc
       61 agatgagatg ttaactgatt tggtgagctt ggagagataa aacaaaataa aggtggtggc
      121 ttaatgccac cccttgaaag gacttttaga aatgattaag gtaaccagaa ttaacggcac
      181 ggatttagtg gttaacgctg acctgataga gtttgtggaa gccaccccgg atactataat
      241 aagcttgatc accgggaaaa agattgtagt gaaggataca attaatgagg tgatagaaaa
      301 agtggcaagt ttcaggaggt tttcttcacc tcaatccacg ctcatcgctc agatgaaaga
      361 gactattaaa gaggccactt aagcacgaat tctgtgtctt gagaaaagtt tccttgaaag
      421 gggatattgt ttatgctaga agtggagaag gccgcaaaaa aagagatgcc cgaagggaaa
      481 gcagaaaaac taaaggaaaa agcacaaaag ggcgaagaag gaaaaccgga gaaggggaag
      541 ttagaagaaa agctggctga ggagccggag agcaagcccg aaggccaatc caagaagggc
      601 ggacttaagc tcaaattgag catacctcct atagttaaaa acatagtcat cttttttgcc
      661 gcttttgtgg ttttggtgat ggcggcttat ctcttgacct caaaagtctt aaaacctttt
      721 atgaaagccc agatacaaaa gagcgctacg cagaaaacca aggcggttgc caagagaaaa
      781 gcccagaaag ttcaaactcc gaacgaagtc aaagaggtct tcttggtcag tgatatgata
      841 ataaatccag ctgaaaccaa tggcacccgc ttcttaaata ccaccatcgg cttaggggta
      901 ggagatccat taaccaaaac tgttctggag gaaagggctc cgcaagtccg ggatgcttta
      961 atctccatct tgaccaccaa gactataccg aaattggttc atcctgatgg aaagaaaatc
     1021 ttgcgtacag agataatgga aaaagtaaat aaaatcattg ctccaaatcg tattttagag
     1081 gtctattttg tcgattttgt gctacaataa ttcagggaga aataacctat gaagacctta
     1141 tctcaggatg agatagacgt cttgctttcc gcggtctcca aaggggatgt tgcaccgagc
     1201 aagaaaaagg aagacaagaa aggcaagaaa tcagcacagg tttatgactt caagcatcca
     1261 gaccgtatct ccaaagacca gatgcgcacc atggagaata tgcatgataa cttgtcagac
     1321 cagcttagct ccatttattc aactctgcag cgcacagtgg tggatgtgga tctgatctca
     1381 gttgaccaga taacttactc cgaatatatc agttccctgc gcatccccag ttgctcttac
     1441 actataagta tggaaccttt agagagagcg gcaatcattg acataagccc atccgtcgcc
     1501 ttctcctttg tggacaggac attcggaggg agtggtcgac ctctgggagt ggagcgggag
     1561 ttgaccggta tcgaaaaaag cgtcatcgat aaagtagtgc accggacact caaggagttg
     1621 aagaaatgct gggaacatat catcaattta gatatgaaat tgatggggtt tgaaagcaac
     1681 cctcaattca ttcagatagt ggctcccagc gaaacggtct tagttatctc cctgcaaata
     1741 aagatattgg atttcagcgg tttgattacc atctgctatc cttatctgac tctggagtcg
     1801 attttgcctc aattaagcgg ccagaactgg gtggacgcca ccaagaagag aaacaccgag
     1861 gtggatagag aaatcaatat gaaaaacatt cagcaggttg ctgctccttt ggtggccatc
     1921 ttaggtcgga cagacatttc catccacgac ttaacccggt tgagcaaggg agatgtgatt
     1981 cgcctggaca attctataaa tgatgagata gagatcttaa ttggcgatag aagcaaatac
     2041 cggggcaagc ccggtttgtg caggaaaaac agagcagtta tgattacttc tatagcttcg
     2101 caagataact tgtaaaggag aggataagat tatggcagac gaagtcaagg ttgagcgaga
     2161 gagagttcct ccttcggagg aaggtttagc tcgagaaaaa tctaaaacag tggagcctga
     2221 aatccgagag caagtcaaaa atggagatga gatggtggag gagccagatg aaccttctga
     2281 aagtgatact ccaaaagaat cttacgagcc accgcttgaa ccgaaacagg tgaaaacagc
     2341 tgaatttcaa caactggcca agggagagaa actgggggag aaacagaata tccagatgat
     2401 tttggatgta actcttccca tttccatcga attgggccac accgctatga gcattggaga
     2461 aatcttggag ctt
//
LOCUS       DASWLN010000136        10046 bp    DNA     linear   ENV 20-FEB-2024
DEFINITION  MAG TPA_asm: Candidatus Zixiibacteriota bacterium isolate
            SMAG_U2949 k141_102744, whole genome shotgun sequence.
ACCESSION   DASWLN010000136 DASWLN010000000
VERSION     DASWLN010000136.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37960594
            Sequence Read Archive: SRR10376091
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Candidatus Zixiibacteriota bacterium (soil metagenome)
  ORGANISM  Candidatus Zixiibacteriota bacterium
            Bacteria.
REFERENCE   1  (bases 1 to 10046)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 10046)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (26-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/12/2024 08:28:26
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 1,440
            CDSs (total)                      :: 1,416
            Genes (coding)                    :: 1,412
            CDSs (with protein)               :: 1,412
            Genes (RNA)                       :: 24
            rRNAs                             :: 1 (16S)
            partial rRNAs                     :: 1 (16S)
            tRNAs                             :: 21
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 4
            CDSs (without protein)            :: 4
            Pseudo Genes (ambiguous residues) :: 0 of 4
            Pseudo Genes (frameshifted)       :: 0 of 4
            Pseudo Genes (incomplete)         :: 3 of 4
            Pseudo Genes (internal stop)      :: 1 of 4
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..10046
                     /organism="Candidatus Zixiibacteriota bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_102744"
                     /isolate="SMAG_U2949"
                     /isolation_source="soil"
                     /db_xref="taxon:2053527"
                     /environmental_sample
                     /country="USA: Oregon"
                     /lat_lon="42.99 N 123.63 W"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            <1..572
                     /locus_tag="VGB16_04570"
     CDS             <1..572
                     /locus_tag="VGB16_04570"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=3
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEX9916992.1"
                     /translation="YAFAGLSIGGTLGYGLGAVKGGLTLGESMSLYSGIAGSGAAIKG
                     IEERSSFGDFWGDYIAGRAIGALYGATGYGYMKLPAGIKWFIRADLMMHTAYQGTLFK
                     KHHGYAGIVLSLLGSYWDFAAVNGGILESDDYLQHVIIQTYVHPNALSMGEEADNIYP
                     SPLHSVTMWIDSHYASDLPGWLQDLLGYK"
     gene            593..1384
                     /locus_tag="VGB16_04575"
     CDS             593..1384
                     /locus_tag="VGB16_04575"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEX9916993.1"
                     /translation="MKKKGLYFLVLFIYFLLNVCIGRGSIFASSDGGLLIGGFGAYVF
                     TDLDLLDYKAKDYRSAKNSATWDMHLQVAYTIKHITPQLDFRLIDETTDGHPGIFSSA
                     IEYPDQELRRAYIRFAPITTSLRFEQRVMRNLEIHYLPGISYLHLETFLKVYDKIAGK
                     DVVILRSSRDFLGPTLSLEPRFSQPISEGENSKKSLFLSSRYAVTFAKEIFHTIYLKL
                     GMQLDPVSENEVKRPRLHTVYVYFNYDINDTFEGYDVGIGWGGLF"
     gene            1427..3352
                     /locus_tag="VGB16_04580"
     CDS             1427..3352
                     /locus_tag="VGB16_04580"
                     /inference="COORDINATES: protein motif:HMM:NF012485.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0019867 - outer membrane [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="secretin and TonB N-terminal domain-containing
                     protein"
                     /protein_id="HEX9916994.1"
                     /translation="MNKFCCLVVSFILFSCIFVQAQEYLQPSQGIKLPDTSSVSSDTT
                     QKSVSESVLADTTIIVPLLDFKEVEIRDILNSLAKAYKLNLWLDPSIVGKTTVYFEKV
                     PLNNILEFIIKENNLEYERTGDIIKLRAKKVIIAPIPQKIEYKDNLLSVDLKGMELDE
                     VVRGLVKETKKNIVVEKGVSGKITGLLERIDFDKGLNALMSTNGFSVNKAENIYYIDK
                     LEQTKGEKVRGGYSVTYNSGMLSLDVTNADLGQLLNEISNHANLDFFVYGDIQGRVSA
                     QCKDLEVEDALRYLFKGTDYTFRKEKEIYFIGNKNMEGVTQTEFIPLKHLVADGVLEL
                     IPKELSSKATLKVVKEQNGIIAIGPYGTISDIKDFIEKIDHPPAQILIEALVVDYSKT
                     DYREFGITANNYGLSSPENLKENYYPDVDVYTHGEEINKNIDKYSSQWKVNNIGHLSA
                     DFFVKLHALEQEGKANIRSRPQIATLNGHKAKIDVGTTQYYLLKTETTYAGNQPTTAT
                     QISQRFETIEASMSLSITPWVTSSQEIIVEIHPEFNTPQGSFDPNVPPTINHRILDST
                     VRLRDGETIVLGGLVQSLENQTIKKFPILGNIPIIGRIFQNRTTTKTNAELVIYLTPH
                     IYYGGEGAVDVTGYEQK"
     gene            3339..4862
                     /locus_tag="VGB16_04585"
     CDS             3339..4862
                     /locus_tag="VGB16_04585"
                     /inference="COORDINATES: protein motif:HMM:NF016992.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="PilN domain-containing protein"
                     /protein_id="HEX9916995.1"
                     /translation="MNKNREESKVDLLSKFKDNKFFYFLRYNMGSMYLDALRGVETSY
                     ALEIDSDNLRLVRANKKDDKVLGIKTDAEPIENLSQSISALFSRHHYSGEEVFISVSG
                     IDFLNKIVEVDLEKNQTVEDWIIKNKDKVFPPTGDEEIEFDYVILEDNERKLLYVSIV
                     RQNRLEQLRQILKENYLNVSSISSGSLGLYHFLKRNEEVARLDKLVLVLLKDKSAEIT
                     FLRKGVPVFTALAGLTEQKEELVSNIRNLMQLYCHSDERTENWKFLILGEGNLASSYE
                     HAFEKAGLSLLEQSWLQKHLDERSVPSTHVIPFSLVDKSGGSISKATTNLLFSHNRLD
                     GYTKSIARVFGYSLKAFLLLLIVLGIFNIGFALFDSLNKDKVKGFESKLETLNDLKQE
                     NQILEEKWGKLSQMLLNRSGESRLLYLISKYRPEGVWLRSISSESISSNQRTEIAGLA
                     VSQAGVSGFFRNLEESNLFEDVKLLYLDRVETTELTKLPAKYKEALFKFKIQFQSKS"
     gene            4865..5419
                     /gene="pilO"
                     /locus_tag="VGB16_04590"
     CDS             4865..5419
                     /gene="pilO"
                     /locus_tag="VGB16_04590"
                     /inference="COORDINATES: protein motif:HMM:NF016253.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_process: GO:0043107 - type IV pilus-dependent motility
                     [Evidence IEA];
                     GO_process: GO:0043683 - type IV pilus assembly [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="type 4a pilus biogenesis protein PilO"
                     /protein_id="HEX9916996.1"
                     /translation="MQSNRLIDFLLNYRRMLILVSGIVVVLVLFLVVDLPLAKLFFEK
                     IHRTYQMSKQYSSLETLDKGIQQEKDLNSEIMKTVSHLQSRKITRNRLAPAIELFNQS
                     AVDSKVSLISVNSSSAIEEENYVKVPFEIELEGEYHHIANFINKIENSSHPLRIESLD
                     ISTRDYISYSLDASLKATLYLVRE"
     gene            5496..6029
                     /locus_tag="VGB16_04595"
     CDS             5496..6029
                     /locus_tag="VGB16_04595"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEX9916997.1"
                     /translation="MDGNAIKKGIFVIGIILLVIIWTRNLGLFSFSPKPYKEAGIEQD
                     AKEKATEGRESKEDKGKVSFSINQMKLTSDFVYQEDYKDPFEAPFFYEKEKRDSLALK
                     ALKAKKDQLPPPKLVLVGIAWDPERPVATIKNELGQTLVVMQKDKIGDVLVDKITREE
                     VYYTFKGRRYKLLISGR"
     gene            6041..7414
                     /locus_tag="VGB16_04600"
     CDS             6041..7414
                     /locus_tag="VGB16_04600"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEX9916998.1"
                     /translation="MMNNEKGSVLITILAVFLIISLLFGSVILYGSWHKFQAQKGLLD
                     LKASYLAEAGVNRAIWCLSGNQDRDIWWRPENYHQDIDKADSYDFTIYNWGSYIAIDS
                     QGKSGGTTKKVKAILGQKPDETYKYAINLGDPAYPLVVTGNSRIIGDVRVGPAGVMPG
                     RFEGEEFIGKKLVDGNILITKDLRLPAYSNKPLEEFAQVIENQKRKPDLTIDRTLVID
                     DKVELNYFKDKCVQVNGDLVFGSSGQTVFKGPGFVWCEGRIEITRNTKLEGGLILFAK
                     KGIILSDDCQVRDCLLYSPSEIVMQDNCRFGSQAISDIGITVKGHATTSYPALLLVKG
                     RKEEKSIEGSISIESDNPVEGILVFCGEVPDTNSKLANNGIVHIKSNADFYGIMYSSG
                     YVTIEGEAFGCINTHRFYLYKSPTTYINWLKDALIDRSKLNKEMILPIGLDNSSGLEI
                     VKYMCLN"
     gene            7417..7776
                     /locus_tag="VGB16_04605"
     CDS             7417..7776
                     /locus_tag="VGB16_04605"
                     /inference="COORDINATES: protein motif:HMM:TIGR02532.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="prepilin-type N-terminal cleavage/methylation
                     domain-containing protein"
                     /protein_id="HEX9916999.1"
                     /translation="MKRISRLNGYTLLEVLISLAIFVSILLPLVTLMATVLNKYSNSD
                     LIVATNLGREEMEKTLNNRTFVDEKNMVKINRISWNISKRIQEDNGLVSITIEVSRRS
                     DDKVLASLYTEKYLGQK"
     gene            7724..8305
                     /locus_tag="VGB16_04610"
     CDS             7724..8305
                     /locus_tag="VGB16_04610"
                     /inference="COORDINATES: protein motif:HMM:NF019575.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="prepilin-type N-terminal cleavage/methylation
                     domain-containing protein"
                     /protein_id="HEX9917000.1"
                     /translation="MIKFWHRFILKNISVRSERGITLVEVLVACALSGIMITFVLYSN
                     LFVNKLIVDWQQKAELEDVGLLCINSLAKDIREADSLVYVSKDRIELISDQGKKTSYY
                     VDGNVLKRNEDELNHGRIALGDIELVCFGRPADAHGSPKDQESLDKNLNLRLDPDELG
                     EVTGIAIKLKLEGYNKSLNLESFVRLNRAYSIY"
     gene            8321..8752
                     /locus_tag="VGB16_04615"
     CDS             8321..8752
                     /locus_tag="VGB16_04615"
                     /inference="COORDINATES: protein motif:HMM:NF019575.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="prepilin-type N-terminal cleavage/methylation
                     domain-containing protein"
                     /protein_id="HEX9917001.1"
                     /translation="MFSKKEYQGGFTLLELMIVVVIIGILAGLAIPQYMSAGTKAKQT
                     EAQQMLKHIFVMEKSYKLNNDSYWIPPEGIAADKEHLNAFVDLGVELMPQVRYAYTIT
                     GDETSFVATAIATSLDDDATIDKWQIDRSGELKVIIDDSKR"
     gene            8952..>10046
                     /locus_tag="VGB16_04620"
     CDS             8952..>10046
                     /locus_tag="VGB16_04620"
                     /inference="COORDINATES: protein motif:HMM:NF014269.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="LON peptidase substrate-binding
                     domain-containing protein"
                     /protein_id="HEX9917002.1"
                     /translation="MAEQENRKTVEKNPDIPAVLPVLPLMTTIIFPLGVTAVQLGFER
                     NTRLIKDNSQQGAVIALVLAKYPTQDKLSVEMISRRGVAAKILNIKEFPKNSIQVTFE
                     GLARINLDEVIQAEPYFRAKISLVVEKEGKTGEVEPLIKKSMDLLEKLIEHDTSYPQE
                     LTHVFNMNVGDASRFADMVASTIHFNLSTKQEILECVDVKERLEKLIQFLQKELEGLR
                     IAEEIDAKVKTTIDKETGTRYLREQLKEIQRELGMVDPVEKEVDELKEKILKTSLPLD
                     VEKQTLLEAERLKLISPASAEYGRIRSYVDWILSLTWKSCGTKDIDIERVEAILNQEF
                     FGQKKVKEKVMEYISILKLKKEFKGAGLCFV"
BASE COUNT         3234 a         1815 c         2321 g         2676 t
ORIGIN      
        1 aatatgcttt tgccggactc agtatcggag gaacactggg ctatggttta ggggcagtca
       61 agggtggtct gaccttgggg gaaagtatgt ccttgtatag cggaattgct ggctcaggtg
      121 cagccataaa aggaatcgag gagcgctcat ctttcggcga cttctgggga gattacatag
      181 caggaagagc aattggtgcc ctatatggag caacaggata cggttatatg aaactaccag
      241 caggaattaa gtggtttatt agagccgatc taatgatgca tacagcatac caaggaactc
      301 tctttaaaaa acaccacggc tacgctggca tagtcttgtc tcttttggga agttactggg
      361 atttcgcagc tgttaacggg ggaatcttgg aatcggatga ttatctccag catgttataa
      421 ttcaaactta tgttcatcct aatgcgttga gtatgggtga agaagcggat aatatttacc
      481 cttcgcccct gcatagtgtt acaatgtgga tagatagcca ctatgcaagt gacctcccag
      541 gatggttgca ggatttgctg ggatacaaat aaaatagtgg aggtatatag tgatgaaaaa
      601 aaagggactc tattttttag tgttgtttat ctattttcta ttgaatgtat gcatcggcag
      661 gggatcaatc tttgctagta gtgatggcgg attgttaata ggtgggtttg gggcgtatgt
      721 ttttacagat ttggacctat tagattacaa agccaaagat tataggagcg ctaaaaatag
      781 cgccacttgg gacatgcatc ttcaggtagc gtatactatc aaacatatta ctccccagct
      841 tgactttagg ctaattgatg aaactacaga cggacacccg ggtatatttt catctgcgat
      901 agaatatcct gatcaagagc taagaagagc atatatacgc ttcgctccaa taactacatc
      961 cctcaggttt gaacaaaggg taatgcgtaa tctggagatc cattacctac ccggcataag
     1021 ttacttgcat ttggagactt ttttgaaagt atatgacaag atagcgggta aggatgtagt
     1081 aatactccgt agttcacgtg attttcttgg accaactctt agcttagagc ctcggttttc
     1141 tcagccaatt tctgaaggtg agaattctaa gaagagcttg tttctttctt caagatatgc
     1201 ggttactttt gctaaagaga tcttccatac aatatatttg aaacttggga tgcaattaga
     1261 tccagttagt gagaatgaag tgaagagacc taggcttcat acagtatatg tctattttaa
     1321 ctacgacatc aacgacacat ttgaaggata tgatgttggt ataggttggg gtggactctt
     1381 ttaggtggtt taggaaaatt cgaaacacat aattagtgag aaggatatga ataagttttg
     1441 ttgtttggta gttagtttta tcttattttc ctgcatcttt gtccaagccc aagagtacct
     1501 acaaccatct caaggaatca aactgcctga cacctcgtcc gtctcctcag atacgacaca
     1561 aaagtctgtt tctgaatcag tcttagccga caccaccatc atcgttccgc ttctggattt
     1621 caaagaggtt gagataagag acatcttaaa ttccttagcc aaagcatata aactgaactt
     1681 atggcttgac ccatcaattg ttggcaaaac cactgtctat tttgagaaag ttcccctaaa
     1741 caatatactt gagttcatca tcaaggagaa taatctggaa tatgagagaa ctggagacat
     1801 aatcaaacta agagcaaaaa aggtcattat agctcccatt ccccagaaga ttgagtacaa
     1861 agataacctc ctctcggttg accttaaagg tatggagctg gatgaagtgg taagagggct
     1921 cgtcaaggag actaagaaaa acatagtcgt agaaaaaggg gtgtctggca aaatcactgg
     1981 cctattggaa agaatagact ttgataaagg cctaaatgct ttaatgagta ccaatggttt
     2041 ctcagtcaac aaggcagaaa acatctacta tatcgataag cttgaacaga caaaaggtga
     2101 gaaagtaaga ggcggttatt ctgtaactta caattctggt atgttgtcct tagatgtaac
     2161 caatgcagac ctgggccagc tcttaaacga gatatcaaat cacgctaatc tggacttctt
     2221 cgtttacgga gacattcagg gtagggtttc agctcagtgc aaggacttgg aagtggaaga
     2281 tgccctgaga tatcttttca aaggtacgga ctatacgttc aggaaagaga aagagattta
     2341 cttcattgga aacaagaaca tggagggggt aacccagact gagtttattc cattgaaaca
     2401 tctggtagcg gacggggtcc tagaacttat tcccaaggag ttatcttcaa aagcaacttt
     2461 gaaggttgta aaagagcaga atggaattat agcaatagga ccatacggga caataagtga
     2521 cataaaagat ttcattgaga aaatagatca cccacctgcc cagatattaa ttgaagcatt
     2581 agtggttgat tactccaaaa cagattacag ggagttcggc ataactgcaa ataactatgg
     2641 tttaagcagc ccagagaacc taaaagaaaa ttactatcct gatgtggacg tttatactca
     2701 cggggaagaa ataaacaaaa atattgacaa atattcttca caatggaagg taaacaatat
     2761 cggacatctc tctgcagatt tcttcgtaaa gctgcatgcc ctggagcagg aaggaaaggc
     2821 taacatccgc tccagacccc agatagctac cttaaatgga cataaagcca agatagacgt
     2881 cggcacaacc caatattatc tcttaaaaac agagaccacc tatgcaggca accagcccac
     2941 caccgcaacc cagataagcc agagatttga aaccattgag gcgtctatga gcttgtccat
     3001 aaccccctgg gttacctcct cacaagagat aatagtagag atacacccag aattcaatac
     3061 ccctcagggt agctttgacc cgaacgtgcc gccgaccata aatcacagga tattggattc
     3121 aaccgtaaga ttaagggacg gagagaccat agttttagga gggttagtcc agtccttaga
     3181 aaatcagact ataaagaagt tccccatctt gggcaatata cccataatcg gtcgaatctt
     3241 ccaaaatagg accaccacca agactaatgc agagctggta atctatttga ccccgcatat
     3301 atattacggt ggagaaggag cggttgacgt aacaggatat gaacaaaaat agagaagaaa
     3361 gcaaggtaga cttattatct aagttcaaag ataataagtt tttttatttt ctaagatata
     3421 atatgggttc aatgtatctg gatgccctta gaggagtcga aacaagttat gctctggaga
     3481 tagactcaga taatctgaga ttagttaggg caaacaagaa agatgataaa gtcttaggaa
     3541 taaaaactga tgctgaacca atagaaaacc tgtcccaatc gatctccgcc ctcttctcaa
     3601 gacaccacta tagtggagag gaagtcttta taagcgtctc cggcattgac tttctgaata
     3661 agatagtcga agtggaccta gaaaagaacc agacagttga ggactggata ataaaaaata
     3721 aggataaggt ttttcctccg actggagatg aggagatcga gtttgattat gttattttag
     3781 aagataatga gagaaaactc ctctacgttt caattgtcag gcagaatagg ttagaacagc
     3841 ttcggcaaat cctcaaagaa aattacctca atgtctccag cataagcagc ggcagcttgg
     3901 gcttatatca ttttctaaaa cgaaacgaag aagtagccag actcgataag ttagtattgg
     3961 tcctattgaa agataaatca gctgagatta ctttcctgcg taagggggtt ccagttttca
     4021 ctgcattggc tggtttgaca gagcaaaaag aggagcttgt atccaatatt agaaatctta
     4081 tgcaattata ttgccatagc gatgaaagaa cggaaaactg gaagtttctg atattaggtg
     4141 aaggaaacct ggcttcatca tatgagcatg cctttgagaa agctgggctg agtcttttag
     4201 agcagagttg gctacaaaaa cacttggacg aacggtctgt tccctcaacc catgttattc
     4261 ctttttcact tgtagacaaa tcaggtggct ctatatccaa agccaccaca aatcttctat
     4321 tttcacacaa caggcttgat ggctacacaa aatcaattgc cagggttttt ggctatagcc
     4381 ttaaagcgtt cttgctcttg cttattgttc ttggaatttt caacatagga tttgccttgt
     4441 tcgatagttt gaacaaagac aaagtcaaag ggtttgaatc caagctggag accttaaatg
     4501 acttgaaaca agaaaaccag atcctggagg aaaagtgggg aaaacttagc cagatgcttc
     4561 tgaatagatc cggagaatcc agacttcttt atctgattag caaatatcgg ccagaggggg
     4621 tttggttaag gagtatatct tctgaaagca tctcctccaa tcaaaggact gagatagcgg
     4681 gattggcagt ttctcaggca ggagtaagcg gtttctttag gaatctagag gagtcaaatc
     4741 tatttgagga tgtcaagcta ttgtatttgg acagggtgga gactactgag ctgactaaac
     4801 tacccgcaaa gtataaagag gcgctgttta agtttaagat tcaatttcaa tctaagtctt
     4861 aattatgcag agcaatagac tcatagattt tctattgaat tacagaagga tgcttatcct
     4921 tgttagtggc atagttgtgg tattggtctt attcttagtc gtggatttac ctctggccaa
     4981 acttttcttt gagaagattc acagaaccta tcagatgagc aaacaataca gttcgcttga
     5041 aacgttggac aaggggattc aacaggagaa ggatctgaat agtgagatca tgaaaactgt
     5101 ttcccatctt caatccagga aaatcaccag gaatagattg gctccggcaa ttgagctttt
     5161 caatcaatca gctgtggata gcaaggtatc tttgatttca gtaaattcct cctcggcaat
     5221 tgaagaagag aactatgtta aagttccctt tgagatagaa cttgagggag aatatcacca
     5281 tatagcaaat ttcataaaca agattgaaaa cagctctcat cctttgagaa ttgagtcatt
     5341 agatatatca acaagagatt acatctctta cagcctcgat gcgagcctga aggcaactct
     5401 ttatcttgtc agggaataag ttatggaggg aagcacagcc aagaagggca aagcgtggct
     5461 gggagccccg cctacgggaa tgagggaata gggttatgga tggaaacgca atcaagaaag
     5521 gcatattcgt catagggata atccttctgg taatcatatg gacgcgtaac ctgggtttgt
     5581 tctctttcag cccaaagcca tataaagagg cgggaataga acaagatgcg aaggaaaagg
     5641 caacagaagg cagggaatca aaagaggaca aaggcaaagt aagcttctca atcaaccaga
     5701 tgaaattgac ctctgatttc gtttatcagg aggactacaa agatcccttc gaagcacctt
     5761 tcttttatga aaaagagaaa agggatagcc ttgctttgaa agctctcaag gcaaagaaag
     5821 accagcttcc tccgcctaaa ttggttctgg ttggcatagc ctgggatccc gaaagacccg
     5881 tggcaacaat taaaaacgag ttgggtcaaa cgctcgtggt tatgcagaaa gataagatag
     5941 gagacgttct ggttgacaag attaccagag aggaagttta ctatactttt aagggaagac
     6001 ggtacaagct gctgatatct ggaagataat taagataaca atgatgaata acgaaaaagg
     6061 ttcagtcctg atcactatcc tggcagtatt tcttattatc tctctgcttt ttggctcagt
     6121 catactttat ggttcctggc acaagtttca ggcgcagaaa ggcctcttgg acttaaaagc
     6181 ctcttatttg gctgaggctg gagtaaatag ggccatctgg tgtctttccg gaaatcaaga
     6241 tagagatatc tggtggagac cggaaaatta tcatcaggat atagataaag ctgactccta
     6301 tgatttcaca atatataact ggggaagcta cattgccata gactctcaag gaaaatcagg
     6361 aggaactaca aagaaagtaa aagccatcct tggccaaaaa ccagatgaga cttacaaata
     6421 tgcaatcaat ttaggggacc ctgcttatcc tctggttgtc actggaaata gcagaataat
     6481 aggagacgtc agagttgggc cagccggcgt tatgcctggg agatttgaag gagaagaatt
     6541 tattggcaaa aaattagttg atggaaatat tttgatcaca aaggacctga gattaccagc
     6601 ttactcaaac aaaccattgg aggagtttgc acaagtaata gaaaatcaaa agagaaaacc
     6661 agatctgaca attgacagga ctctggttat agatgataaa gttgaactga attactttaa
     6721 agataagtgc gtgcaggtca atggtgattt ggtttttggc tcttcgggtc agacagtttt
     6781 caaaggcccg ggatttgtat ggtgtgaagg cagaatagag ataaccagaa acacaaagct
     6841 cgaaggcggg ttgatcttat ttgccaagaa gggaattatc ctctcagatg attgtcaggt
     6901 cagagactgc ctgctttatt ctccatcaga gatagttatg caggataatt gcaggtttgg
     6961 gtcgcaagcc atctcagata taggtataac tgttaaaggt catgccacca catcctatcc
     7021 tgcgctgctt ttggtcaaag ggagaaagga agagaaaagc atagagggaa gcatatccat
     7081 tgaaagcgat aacccagttg aggggatatt ggttttctgc ggagaagtgc cagataccaa
     7141 ttccaaatta gcgaataacg gcatcgtcca tataaagtca aatgctgatt tttatggaat
     7201 tatgtattct tctggttatg tgacgattga aggggaggcc tttggttgta ttaatacgca
     7261 taggttctac ttgtacaaga gcccgaccac ttatatcaac tggctcaaag atgcgttgat
     7321 agataggtcc aagttaaata aggagatgat cttacccatt ggtttggata actcatccgg
     7381 gcttgagatt gtaaaataca tgtgcttgaa ttaaacatga aaaggatttc aagattaaat
     7441 gggtatacgt tacttgaggt tttgatatcc ttggccatat tcgtatcgat acttttgcct
     7501 ttggtaaccc tgatggcaac ggtcttgaac aagtattcta attcggatct aatcgttgct
     7561 accaacttgg gcagagaaga aatggaaaaa actctcaaca atagaacatt cgtagatgaa
     7621 aagaatatgg taaaaataaa taggatatcc tggaacatat caaagagaat ccaggaggat
     7681 aacgggcttg tgtcaattac catagaggtt tcgaggagaa gtgatgataa agttctggca
     7741 tcgctttata ctgaaaaata tctcggtcag aagtgagcgg ggcataacct tagttgaggt
     7801 tctggtagcc tgcgctctat ctggtataat gatcactttt gtgctctact ccaatctttt
     7861 cgtcaataag ttaattgtag attggcagca aaaagcagag ctcgaggatg ttggcttgct
     7921 ttgtattaac tctttggcta aggatatccg ggaggctgat tctttggtgt atgtttcaaa
     7981 agacaggata gagctgatca gtgaccaggg gaagaagaca tcctattacg tggatggaaa
     8041 cgtcttaaaa agaaatgagg acgaattaaa ccatggccga attgctttag gcgacattga
     8101 actggtctgt tttggtagac cagctgatgc tcacggttcg cccaaagacc aggaatcgct
     8161 cgacaagaac ttgaatctta ggctcgaccc ggatgagcta ggtgaggtta cgggcatcgc
     8221 aataaaatta aagctggaag gatacaacaa aagcttaaac ctggagagtt tcgtcagatt
     8281 aaatagggct tattcaatat attgacaaag gagaaggagc atgttctcga aaaaagagta
     8341 tcagggtggt tttaccctgc ttgaacttat gatcgtggtg gtgataatcg ggattctggc
     8401 aggactggct attcctcagt atatgagcgc aggaacaaaa gcaaaacaga cagaagccca
     8461 gcagatgctc aaacatatct ttgtgatgga aaaatcctat aagctcaaca atgactccta
     8521 ttggatacct cctgaaggaa tagctgctga taaagaacat ctaaatgctt ttgtggatct
     8581 gggggtggag ttgatgcccc aggtgagata tgcttacacc ataaccggag acgaaacctc
     8641 cttcgttgcc acagccattg ccaccagctt ggatgatgat gctacgattg acaaatggca
     8701 gatagaccga agcggagagc taaaggtaat tattgatgat tctaaaaggt aatgggtcta
     8761 attcagtgta aaattgtaat ttctcgcctt ggagatttat cagcttcttc aagtaatatg
     8821 agtcttcact tctcgaacac agtcacaaat atatcttgac aattacccag atcgggctta
     8881 ataatcgaag ataaaccaaa gagttaatct gacgatataa taattgggcc caaagttatc
     8941 ggagaatcga aatggctgag caggaaaata gaaaaacagt cgaaaagaac ccggatatac
     9001 cagctgtact tccagttcta cctcttatga ccaccatcat tttcccttta ggggtgactg
     9061 cagtccagtt aggttttgag agaaacactc gcttgattaa agataactcc cagcaaggtg
     9121 ccgtgattgc tttagttttg gctaagtatc ctacccagga taagctcagc gtggagatga
     9181 tatcgcgtcg cggagtagcc gcaaagatct tgaatattaa ggaatttccc aaaaattcca
     9241 tccaggtgac atttgaaggt ttagctcgca ttaacctgga tgaggtaatt caagccgaac
     9301 cttacttcag agcaaaaatt agtctcgttg tggaaaagga agggaagaca ggtgaagttg
     9361 aacctctgat taagaaaagc atggatttgc tggagaagct gatagagcat gacactagtt
     9421 atccacaaga gcttacccat gtgttcaata tgaatgtagg agacgccagc agatttgcag
     9481 atatggtagc ttccaccatt cactttaacc tcagcactaa gcaggagata ttagaatgtg
     9541 tggatgtcaa ggaaagattg gagaaactga tccagtttct acaaaaagag ctagagggat
     9601 tgaggattgc ggaagagata gatgcaaaag tcaagaccac catagataag gaaaccggaa
     9661 cccgctatct gcgggagcaa ttaaaagaga ttcaaaggga attgggcatg gtggatccgg
     9721 tggagaagga agtagatgag cttaaagaga aaattctcaa aacctctttg cctttagatg
     9781 ttgaaaaaca gactctcctg gaggcggaaa gacttaaact catatctcca gcttcagctg
     9841 aatatggcag gataaggagt tatgttgatt ggatcttatc acttacctgg aagagttgcg
     9901 gaacaaaaga tatcgacatt gagcgggttg aggcgattct aaatcaggaa ttctttggcc
     9961 agaagaaagt aaaagaaaag gtgatggaat atatctcaat ccttaagttg aagaaagaat
    10021 tcaagggtgc aggattgtgt ttcgtg
//
LOCUS       DASWLN010000137         2621 bp    DNA     linear   ENV 20-FEB-2024
DEFINITION  MAG TPA_asm: Candidatus Zixiibacteriota bacterium isolate
            SMAG_U2949 k141_1000455, whole genome shotgun sequence.
ACCESSION   DASWLN010000137 DASWLN010000000
VERSION     DASWLN010000137.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37960594
            Sequence Read Archive: SRR10376091
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Candidatus Zixiibacteriota bacterium (soil metagenome)
  ORGANISM  Candidatus Zixiibacteriota bacterium
            Bacteria.
REFERENCE   1  (bases 1 to 2621)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 2621)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (26-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/12/2024 08:28:26
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 1,440
            CDSs (total)                      :: 1,416
            Genes (coding)                    :: 1,412
            CDSs (with protein)               :: 1,412
            Genes (RNA)                       :: 24
            rRNAs                             :: 1 (16S)
            partial rRNAs                     :: 1 (16S)
            tRNAs                             :: 21
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 4
            CDSs (without protein)            :: 4
            Pseudo Genes (ambiguous residues) :: 0 of 4
            Pseudo Genes (frameshifted)       :: 0 of 4
            Pseudo Genes (incomplete)         :: 3 of 4
            Pseudo Genes (internal stop)      :: 1 of 4
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2621
                     /organism="Candidatus Zixiibacteriota bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_1000455"
                     /isolate="SMAG_U2949"
                     /isolation_source="soil"
                     /db_xref="taxon:2053527"
                     /environmental_sample
                     /country="USA: Oregon"
                     /lat_lon="42.99 N 123.63 W"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            complement(27..1235)
                     /locus_tag="VGB16_04625"
     CDS             complement(27..1235)
                     /locus_tag="VGB16_04625"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_006440159.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="pyridoxal phosphate-dependent aminotransferase"
                     /protein_id="HEX9917003.1"
                     /translation="MNKRLSISSRADMMPYSPIRKLVPLAYQAKDKGIKVCHLNIGQP
                     DIPTPPQFMRAVRSYKQKVLAYGDSQGDREYLKALAEYYKRRSIEVKPQDIVVTTGGS
                     EAIIFAMLCVADTDEELIVFEPFYPNYNGFARMADVKLVPITLRAENGFHLPSRSEIE
                     KKITKKTRAILVCSPNNPTGTVYTRKEMEMLAHICKKNNLFLLSDEVYREFIYEGKHT
                     SILHLKGMEKRGIIIDSISKRFSACGARIGCLVSKNPEVINSAWRMGQARLCVPSIEQ
                     FAAKNALTINDSYFKKMTKEYKRRRDAAYQELMRIPGTVCVKPGGAFYIMAKFPITDI
                     EHFAKWMLTDFSLDGVTTMIAPGPGFYCTPNRGKDEARIAYVLNVKDLRKAMKILASG
                     IKVYNSIGKK"
     gene            complement(1363..1800)
                     /locus_tag="VGB16_04630"
     CDS             complement(1363..1800)
                     /locus_tag="VGB16_04630"
                     /inference="COORDINATES: protein motif:HMM:NF012301.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_process: GO:0000160 - phosphorelay signal transduction
                     system [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="response regulator"
                     /protein_id="HEX9917004.1"
                     /translation="MELKDASLDKVGTITTGKKTRVAKILVIDDEPEITDIVEAFLKN
                     AGYEVRVENSSVMGIEKAKSFKPDLILLDIMMPRMDGYQVCNELKKNSETKNTPIIFL
                     TGKDASHDEGKSFKVGGDMFIKKPFSCQRLLEIVKIVLISISK"
     gene            complement(1804..2592)
                     /locus_tag="VGB16_04635"
     CDS             complement(1804..2592)
                     /locus_tag="VGB16_04635"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_015424699.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_process: GO:0006807 - nitrogen compound metabolic
                     process [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="nitrilase-related carbon-nitrogen hydrolase"
                     /protein_id="HEX9917005.1"
                     /translation="MRVGFIQLQPVFGQKKVNLEKAIKLIQQVEADLLVLPELFNSGY
                     LFLEKEEVQNLAERIPQGETTSILREIAKERKVSLVFGIAEEHKGKFFNSSVLLEPGG
                     RVHIYRKLHLFFEEKLFFSQGNRKLEVIELEGAKVGMMVCFDWIFPEVARSLTLKGVD
                     IICHPANLILPHCQEAMRTRCIENRVFAITANRTGRESRGGKELFFTGKSQILNPLGE
                     VLTQASRDKEEVRVVEIDPQMARDKNVTLYNNLFGDRRPKFYKR"
BASE COUNT          707 a          625 c          478 g          811 t
ORIGIN      
        1 tccaatgtaa tagtcttttt tcgaaatcat tttttcccta tgctgttata gactttaatt
       61 ccagaagcca aaatcttcat tgcttttctt aaatccttca cattcaaaac ataggcgatt
      121 cgggcttcat cttttcctcg gttgggagtg caataaaatc cgggaccggg agcgatcata
      181 gtggttactc catccaaaga gaaatcagtt agcatccact tcgcaaaatg ctcgatatcc
      241 gttataggga acttagccat aatgtaaaaa gcaccgccgg gctttacaca gaccgtgccc
      301 ggaatcctca tcaattcctg atatgccgca tcccttcttc tcttatactc tttggtcatc
      361 ttcttgaaat atgaatcatt aattgttaaa gcatttttag cggcgaactg ttcgattgag
      421 ggaacgcaca aacgagcttg ccccattctc caggcagagt taatcacctc ggggttttta
      481 ctcaccagac atccgattcg agcaccacag gcgctgaacc tcttggagat gctgtcaata
      541 ataatacctc ttttctccat ccctttcaaa tgcaaaatgc tggtatgttt tccttcatag
      601 ataaactcac gatacacttc atctgagagg agaaagagat tatttttctt gcagatgtgc
      661 gccagcatct ccatctcttt tcgggtataa acggttccag tcggattatt aggtgagcaa
      721 accagaatag cacgagtttt cttagttatc tttttttcta tttcacttct ggatggaagg
      781 tgaaaaccat tttctgcccg taaggtgatg ggcacgagtt taacatccgc cattcgggca
      841 aaaccgttat aattgggata aaatggctca aagacaataa gttcctcatc agtatcagct
      901 acacacaaca tcgcaaagat tatggcttca cttcctccag tggtaacaac gatgtcctgg
      961 ggtttaactt ctatactcct tcttttgtag tattccgcta aagctttcag atactcccta
     1021 tccccttgag aatcgccata agctaagacc ttctgcttgt aacttcttac cgcccgcata
     1081 aactgcggag gagttggtat atccggttgc ccgatattca aatggcaaac tttaattcct
     1141 ttatctttag cttgataagc aagcgggacc aattttctga taggagagta aggcatcata
     1201 tcagcgcgtg aggaaataga aagtctctta ttcataagaa gtctccttaa ttaaatttct
     1261 caaatttagc aaaacaaaag gggattgtca aatgaaatag aatgagataa aatatagtgc
     1321 gtccgaggaa atgtatgtgg aataaaatag ggaaagcagg gtttacttgc ttatactaat
     1381 caacacaatc ttaactatct ccaggagcct ttgacagctg aacggttttt tgatgaacat
     1441 atccccgccc accttgaagc tttttccctc atcgtggctg gcatcttttc cagtcaagaa
     1501 gatgatcggg gtatttttag tttcggagtt tttctttagt tcattgcaga cttggtatcc
     1561 atccatcctc ggcatcataa tatccaaaag gatcaaatcc ggcttaaagc ttttggcttt
     1621 ctcaattccc atcacgctgg agttctcaac tctaacctca taccccgcat ttttcaaaaa
     1681 agcctcgacg atgtcagtta tctccggctc atcatctata accagtatct tggcaaccct
     1741 tgtcttctta ccggtggtga tggtgccgac tttgtctaag ctcgcatctt ttagttccat
     1801 atatcacctt ttataaaatt ttggtcttct gtccccaaag agattgttat ataaagtgac
     1861 atttttatcc ctcgccatct gggggtctat ctctaccact cttacctctt ctttatctcg
     1921 gctagcttgg gtcaaaactt cacctaaagg attaagaatt tgactttttc cggtgaaaaa
     1981 aagctctttg ccccctcggc tttccctgcc tgttctgttg gcagtgatgg cgaaaaccct
     2041 gttttcgatg cagcgcgtcc tcatcgcctc ctgacagtgg ggtaatatca ggttagccgg
     2101 atgacagatt atatccacac ctttcaaagt caagctcctc gccacctctg gaaaaatcca
     2161 gtcaaaacag accatcatcc ccaccttagc cccttccagt tcaattacct caagctttct
     2221 attcccctga gagaagaaaa gtttttcctc gaaaaaaaga tgaagcttgc ggtaaatatg
     2281 aactctccct ccgggttcaa gcaacaccga ggagttgaag aacttacctt tgtgctcttc
     2341 tgcaatgcca aagaccagac ttacttttct ttcttttgca atttccctta agatagaggt
     2401 ggtttcgcct tgtggtatcc tttcggctaa gttttgcact tcctctttct ccagaaagag
     2461 ataaccagag ttgaaaagtt caggcaaaac caaaaggtca gcctcaacct gttgtataag
     2521 cttgatggct ttttccaagt tgactttctt ttgcccaaac acaggttgaa gttgaataaa
     2581 cccgaccctc aaagctcctc ctgttaattc attcaactaa t
//
LOCUS       DASWLN010000138         2703 bp    DNA     linear   ENV 20-FEB-2024
DEFINITION  MAG TPA_asm: Candidatus Zixiibacteriota bacterium isolate
            SMAG_U2949 k141_1079248, whole genome shotgun sequence.
ACCESSION   DASWLN010000138 DASWLN010000000
VERSION     DASWLN010000138.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37960594
            Sequence Read Archive: SRR10376091
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Candidatus Zixiibacteriota bacterium (soil metagenome)
  ORGANISM  Candidatus Zixiibacteriota bacterium
            Bacteria.
REFERENCE   1  (bases 1 to 2703)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 2703)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (26-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/12/2024 08:28:26
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 1,440
            CDSs (total)                      :: 1,416
            Genes (coding)                    :: 1,412
            CDSs (with protein)               :: 1,412
            Genes (RNA)                       :: 24
            rRNAs                             :: 1 (16S)
            partial rRNAs                     :: 1 (16S)
            tRNAs                             :: 21
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 4
            CDSs (without protein)            :: 4
            Pseudo Genes (ambiguous residues) :: 0 of 4
            Pseudo Genes (frameshifted)       :: 0 of 4
            Pseudo Genes (incomplete)         :: 3 of 4
            Pseudo Genes (internal stop)      :: 1 of 4
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2703
                     /organism="Candidatus Zixiibacteriota bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_1079248"
                     /isolate="SMAG_U2949"
                     /isolation_source="soil"
                     /db_xref="taxon:2053527"
                     /environmental_sample
                     /country="USA: Oregon"
                     /lat_lon="42.99 N 123.63 W"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            764..1933
                     /locus_tag="VGB16_04640"
     CDS             764..1933
                     /locus_tag="VGB16_04640"
                     /inference="COORDINATES: protein motif:HMM:NF025690.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF4388 domain-containing protein"
                     /protein_id="HEX9917006.1"
                     /translation="MSFTGNLSTVSFADLLQLISTSSKSGRLNVTKGNQQKNVYFDKG
                     GIVFASALNSDSTLLGNLLLRKGRISKPNLEKAISIHRVSGKKLGKVLVEMRLLNEED
                     ITEWLRLQIEEIVYSLFGWDSGEFVFYDGEAPPKDQSTTQLNTMNVIMEGTRRIDEWI
                     EIQKLLPDGDTILKLVAHPNLANDSVKFTPEEYRTFLLINGERTLPEIVELSPYGEFI
                     TSKAIYKFIVSGLVEKGKKRAPKKSEKEEDETLLALLSTIFSTCFSLIDQELTDKVGQ
                     GKDKILEKSLGSEKIYHPLLGEMLSSPAEVDMENFNKVASKLPEKLRFCHIFNSLNSL
                     LTSYLETCSLILGKNITRKLISELKKGIGPLISENKELDKKYGIEEDLFRRLRMI"
     gene            1954..2331
                     /locus_tag="VGB16_04645"
     CDS             1954..2331
                     /locus_tag="VGB16_04645"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEX9917007.1"
                     /translation="MDKVKKITLLVISLLLLETGYANADFRELIDQPTPMIVLSVLLL
                     LLTVAGFWFSSRIRAFLRGGELIVGWFLILVSFLFLFGSQVLELGRYLDVFEVSLTVV
                     YVAKLFWLMILILGIYFIQKVMS"
     gene            2419..>2703
                     /locus_tag="VGB16_04650"
     CDS             2419..>2703
                     /locus_tag="VGB16_04650"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEX9917008.1"
                     /translation="MINHQDLDDRISKCQRILTDNPKSQIFAALADAYRRKGGLDKAF
                     QVCAKGLEIHPNYGPAHVVMAKIYMYQGKYDLAEREVNIAIQADGATRATE"
BASE COUNT          816 a          490 c          654 g          743 t
ORIGIN      
        1 ggcgagggga gatttattga acgtaaatta atgagtttgt attagattta ttcaaaagcg
       61 atattttccc cttgaactgt agagctcctt gcttggttta tgctcttggt taattgcatt
      121 gtgcagggac gaatccaagg aattcattcg caggaccaca ttaaataagt ttttaacctc
      181 acctaacctt cttcttatcg agaccatgtt atagacctac ttctcaagat aatcacacga
      241 tattttgtag cggtcggatt tatccgacgc cgtattttgt gtgcgataaa tcgcaccgct
      301 acatagccac tctaaaggag aggataaagg tgaggatata tcaggaatta tataaagaac
      361 cccgcccaaa tggtagggca ttaaagccca aatcgagatc ccgaaacaag ttcgggatga
      421 caagaaaaga aacaagttca agatgacaga aaccgtaaaa ccaaaacccg gttgtcatac
      481 tgaactcgtt tcagcatcta gtctgttttt ctttgcttcc atagatatgc acctttcatc
      541 gccgtccacg aagacgaggt tttcaggtgc gatcttgata agttagccat cctgtatggg
      601 aagtttccca cacttttatg ttgccttgtg ggttaagatt ttgtttattt ctcccgataa
      661 acaaatagaa tagaggagat tatatgtggg caaaaagctc gcatacattt ctccttaagt
      721 tagaggataa atacaagttc agaaaaacag cccagggaga cagatgtctt ttactggaaa
      781 cctaagtact gtatcctttg cagacctgct tcagctgatt agcacctcta gcaagagtgg
      841 acgactcaat gtcactaagg ggaaccagca gaagaacgtc tattttgata aggggggcat
      901 agtgttcgcc agtgccctaa atagtgacag cactcttctg ggcaatcttc ttttaagaaa
      961 agggagaata tccaagccca atttggagaa ggcaataagc atccaccgag tatctggcaa
     1021 gaagttgggc aaagtattgg tggagatgag gctcctcaat gaggaggata taacagaatg
     1081 gttaagactt caaatagagg agatcgtcta tagtctattc ggatgggatt caggagagtt
     1141 cgttttttat gacggggagg caccgccaaa ggaccagagt accactcagc ttaacaccat
     1201 gaatgtaatc atggagggaa ccagaagaat agatgaatgg atagaaatcc aaaaattact
     1261 tccggacgga gacaccattc tgaagttagt tgctcacccc aatttggcaa acgactctgt
     1321 caaattcact ccggaggaat ataggacttt cttgttgata aacggcgaga ggactctacc
     1381 cgaaatagta gagctaagcc cttatggcga attcatcact tccaaggcga tttacaaatt
     1441 cattgtatct ggtttagtgg aaaagggaaa gaagagggca cccaagaagt cggaaaagga
     1501 agaggatgaa acactcttag cccttttatc cacaatattc tctacctgtt tttccctgat
     1561 tgaccaggag ctgacagata aagttgggca aggcaaggac aagattttag agaagagtct
     1621 gggtagcgag aagatatatc atcccctgct gggcgagatg ctctcttcac ccgcggaagt
     1681 agatatggag aacttcaaca aggtagcttc taagctcccg gaaaaattga gattctgtca
     1741 tatcttcaat agtttgaatt cgcttctgac aagttatctg gaaacctgtt ctctgatttt
     1801 gggaaaaaac ataacccgca agctaatctc cgagctgaaa aagggaatag ggccgctcat
     1861 ttctgaaaat aaggaattgg ataagaaata tggtatagag gaagatttgt tccgcagatt
     1921 gagaatgata tagctcaaaa aagaggattt gatatggaca aagtaaaaaa gataacgttg
     1981 ttagttataa gcttgctgtt gttggagaca ggttatgcga atgccgactt ccgggagttg
     2041 attgaccagc caactccaat gattgtgctt agtgtattat tattgcttct gaccgtggct
     2101 ggattttggt tctcttcgcg catcagggct ttcttgagag gaggagaact gattgtggga
     2161 tggtttctga ttctggtttc atttttattc ttatttggtt cgcaagtttt ggaattagga
     2221 agataccttg acgtctttga ggtgagcctc acagtggtat atgtggcgaa gttgttttgg
     2281 ttaatgatat tgattctggg gatatatttc atacagaagg tgatgagtta gcccaaatat
     2341 ggggagaaaa ggaacttaat acgtaaaact gcttgtcatg gaagacattg agattatgtt
     2401 gctcaaaaga ggtattatat gattaaccat caagacctgg atgataggat aagcaagtgc
     2461 cagaggattt tgacagataa ccctaaatcg cagatcttcg ccgctctggc ggatgcctat
     2521 cgtcggaaag gtgggttaga taaggccttt caggtctgtg ctaagggatt ggagattcat
     2581 ccgaattatg gcccagccca tgtggtgatg gctaagatct atatgtatca ggggaagtat
     2641 gatttagcgg aaagagaggt taatattgcc attcaggcgg atggggcaac gcgcgccacg
     2701 gag
//
LOCUS       DASWLN010000139        10879 bp    DNA     linear   ENV 20-FEB-2024
DEFINITION  MAG TPA_asm: Candidatus Zixiibacteriota bacterium isolate
            SMAG_U2949 k141_1002125, whole genome shotgun sequence.
ACCESSION   DASWLN010000139 DASWLN010000000
VERSION     DASWLN010000139.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37960594
            Sequence Read Archive: SRR10376091
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Candidatus Zixiibacteriota bacterium (soil metagenome)
  ORGANISM  Candidatus Zixiibacteriota bacterium
            Bacteria.
REFERENCE   1  (bases 1 to 10879)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 10879)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (26-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/12/2024 08:28:26
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 1,440
            CDSs (total)                      :: 1,416
            Genes (coding)                    :: 1,412
            CDSs (with protein)               :: 1,412
            Genes (RNA)                       :: 24
            rRNAs                             :: 1 (16S)
            partial rRNAs                     :: 1 (16S)
            tRNAs                             :: 21
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 4
            CDSs (without protein)            :: 4
            Pseudo Genes (ambiguous residues) :: 0 of 4
            Pseudo Genes (frameshifted)       :: 0 of 4
            Pseudo Genes (incomplete)         :: 3 of 4
            Pseudo Genes (internal stop)      :: 1 of 4
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..10879
                     /organism="Candidatus Zixiibacteriota bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_1002125"
                     /isolate="SMAG_U2949"
                     /isolation_source="soil"
                     /db_xref="taxon:2053527"
                     /environmental_sample
                     /country="USA: Oregon"
                     /lat_lon="42.99 N 123.63 W"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            complement(536..1585)
                     /locus_tag="VGB16_04655"
     CDS             complement(536..1585)
                     /locus_tag="VGB16_04655"
                     /inference="COORDINATES: protein motif:HMM:NF020123.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016209 - antioxidant activity [Evidence
                     IEA];
                     GO_function: GO:0016491 - oxidoreductase activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="redoxin domain-containing protein"
                     /protein_id="HEX9917009.1"
                     /translation="MKIRNSFLLALIAIFLVLNVYLFLRHGSMTKPIPYKPQAELPGI
                     KNEEVAPAFVLKDLEGISHSTDELKGKITLLIFREIKDEKSFLEAYYYKLLLGKYRDK
                     GFVVWIISTAKDSLAVKDVAKVYSPILVLKDKDGEITKKYCNKEIEGFRAFIIDRDGI
                     VKFRDYNLPNILVRMTVDRFVLNEEKLLGEDEFVKGQNLPSLEYYDVKDNSIKKLADF
                     VGKPILLTLFSANCPTCREHKRLLLMQSAYEEYGPKGLRVVLVYGKDNPLSIIRDYVQ
                     KAKLSYDVGVRKVEPEESDSYYKNYDLEVDPKSIIVTSDGKVIFIEDLKDTQENISKA
                     IDELFKDNKLRKKNK"
     gene            complement(1748..3481)
                     /locus_tag="VGB16_04660"
     CDS             complement(1748..3481)
                     /locus_tag="VGB16_04660"
                     /inference="COORDINATES: protein motif:HMM:NF015052.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="1,4-alpha-glucan branching protein
                     domain-containing protein"
                     /protein_id="HEX9917010.1"
                     /translation="MAIGSFAFVLHSHLPYVIAHGKWPHGMDWLNEASAETYIPLLNI
                     LNELVEEGYSPKLTIGITPVLLEQLADPTFKEELSDYLEQKIKAAEDDVVEFQRYKKE
                     NLLKLALKWTEYYSKINDDFENKYKRDLIAAFKTLQDSDHIEIITSAATHGYLPLLSE
                     DTSVQAQIKLGAQVYEKHFGKESRGIWLPECAYRPRYNWTSPVETSIGKPNHLRKGVD
                     EFLSENGIDYFIVDSALLRGGKAIGVYVDRFEALKQLWGRFEKEYQPREEEFDKTPRE
                     VYLVNSGGEGKKPVAIFTRDPDTGLQVWSGEWGYPGDGNYLDFHKKHFPGGHRYWKIT
                     SAKSDLADKMEYYPPDADRMVPENATHFKGLVKRLLVEYYQKTKQKGIVCAPYDSELF
                     GHWWFEGTKFLKLVLENIADDPEIELTTCSKFLDESKPSKVISIPEGSWGEGGYHYIW
                     LNKWTEWTWKHIYEDELWMQTLAEKFKETGDENLNKILKQLARELLLMEASDWQFLIS
                     TWSARDYAELRLSEHHNNFVRLASMAEKYARGEKLDEGEWTFLGDCEKRDKVFPELKL
                     RWWANVEYPAK"
     gene            complement(3562..4320)
                     /locus_tag="VGB16_04665"
     CDS             complement(3562..4320)
                     /locus_tag="VGB16_04665"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEX9917011.1"
                     /translation="MMTKILETILNKIDLSDIIIAILTIAGWIIAFIIMKNEVKKTHE
                     KNLKLQDKLLARQVEYEAYKELQRTIRPFNESLIDFYTYLAILRSDIELTHKTTSPFV
                     CPSCDWETVHTKLLELMELSGSKLRNFSFTYDFNQIVFPELRETTLKIGDAYLSFADN
                     ESDFFAFYPINCSPDNKIPLKEKQKEFNDKINSLTKKLIGIMAGLEDLSIELQNKVLG
                     TVLDRSINKEELIKISINTLKRSGEDELRPYANQ"
     gene            complement(4408..4845)
                     /locus_tag="VGB16_04670"
     CDS             complement(4408..4845)
                     /locus_tag="VGB16_04670"
                     /inference="COORDINATES: protein motif:HMM:NF013921.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003677 - DNA binding [Evidence IEA];
                     GO_function: GO:0004803 - transposase activity [Evidence
                     IEA];
                     GO_process: GO:0006313 - DNA transposition [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="transposase"
                     /protein_id="HEX9917012.1"
                     /translation="MTKLPKRRSSLRLKGFDYSSEGCYFVTINCRENRKCFAKNGIRD
                     IIKNNIEKLNGAFSLTIEGCAIMENHLHLMITIKNRNNTNLSKVIQSFKSLIAKEVRE
                     RVGIYDKIWQRGFYDHIIRNEKDYREKMEYITNNPLKFELGRR"
     gene            complement(4947..5240)
                     /locus_tag="VGB16_04675"
     CDS             complement(4947..5240)
                     /locus_tag="VGB16_04675"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_006929692.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF3467 domain-containing protein"
                     /protein_id="HEX9917013.1"
                     /translation="MEQKKKLNIELGEKESEGIYSNLVLIVHSPSEIIFDFARIMPGV
                     PKTKLYARIIMTPQHAKMFLKNLEESIKNFENEFGKINLPDKEDMQIGFQKKE"
     gene            5430..6440
                     /gene="rfaE1"
                     /locus_tag="VGB16_04680"
     CDS             5430..6440
                     /gene="rfaE1"
                     /locus_tag="VGB16_04680"
                     /inference="COORDINATES: protein motif:HMM:TIGR02198.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016773 - phosphotransferase activity,
                     alcohol group as acceptor [Evidence IEA];
                     GO_process: GO:0009244 - lipopolysaccharide core region
                     biosynthetic process [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="D-glycero-beta-D-manno-heptose-7-phosphate
                     kinase"
                     /protein_id="HEX9917014.1"
                     /translation="MRKIKLARLEKIFSNLGGHKILVVGDVMLDEYLWGNVSRISPEA
                     PVPVVEVTRETIKLGGAANVALNIETLGAEPILVGLVGKDKNGEKLRSLLKSLRISDE
                     GILVDKNRPTTSKTRIIAHHQQVVRADRENIEEMNSETCKRILRFANDKMKEIDAIII
                     SDYGKGMITLNLLNELIFLARKDRKFIAVDPNKETHFINYKKVSIITPNLDEASFAFG
                     KRIKDEKSLEEVGWGLLERLEVESILVTRGEKGMSLFQRDKTLTHFPTVAKKVYDVTG
                     AGDTVISAFSVAYCAGADLKESALISNHAAGIVVGEIGTGQVDKKTLLEDLKDFVNGN
                     ED"
     gene            6442..6921
                     /gene="rfaE2"
                     /locus_tag="VGB16_04685"
     CDS             6442..6921
                     /gene="rfaE2"
                     /locus_tag="VGB16_04685"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_012963286.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016779 - nucleotidyltransferase activity
                     [Evidence IEA];
                     GO_process: GO:0009244 - lipopolysaccharide core region
                     biosynthetic process [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="D-glycero-beta-D-manno-heptose 1-phosphate
                     adenylyltransferase"
                     /protein_id="HEX9917015.1"
                     /translation="MGQVVKLNRLIRIREKARKARKRIVFTNGCFDILHRGHIDFLKK
                     AKRLGDILIVGLNTDSSVKKIKGKQRPIISQNDRAEILANLLPVDYVCLFHEETPRRL
                     ISALMPDVLVKGRDYKKRGIVGKDIVELSGGKVVRIKLVQGKSTKHIINTVLNRYKR"
     gene            6991..8064
                     /locus_tag="VGB16_04690"
     CDS             6991..8064
                     /locus_tag="VGB16_04690"
                     /inference="COORDINATES: protein motif:HMM:NF012766.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008233 - peptidase activity [Evidence
                     IEA];
                     GO_process: GO:0006508 - proteolysis [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="Xaa-Pro peptidase family protein"
                     /protein_id="HEX9917016.1"
                     /translation="MKNRIRRIVSLLEKENLDALLVNFQPNVRYLSGYDGTNGLVVLA
                     SDKRLFITDFRYQEQAQKEVKDLKLHFAQRELIYDLAKFKFLQKRNQRIGFEANHTSY
                     STVQKLKEIIPNALLVPTENLVESLLIIKDKGEVAKIEKAAQITDKTFSHILDLVKPG
                     VREKDLAAEIEHSFKRMGGDGAAFSTIVASGYRSSMPHGRASEKKLKVGEFVTFDMGA
                     IYQGYVSDMTRTLVLGKATLKQRKIYSLVLSAQTKAISRAKAGISGMELDKVAREVIA
                     KAGYGKYFGHGLGHGLGLEVHSGPMVNARSKTILKPGMVITIEPGIYIPGWGGVRIED
                     DVLITARGCKVLNQSPKNLIEIR"
     gene            8072..8548
                     /gene="accB"
                     /locus_tag="VGB16_04695"
     CDS             8072..8548
                     /gene="accB"
                     /locus_tag="VGB16_04695"
                     /EC_number="6.4.1.2"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_006930212.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0009317 - acetyl-CoA carboxylase complex
                     [Evidence IEA];
                     GO_function: GO:0003989 - acetyl-CoA carboxylase activity
                     [Evidence IEA];
                     GO_process: GO:0006633 - fatty acid biosynthetic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="acetyl-CoA carboxylase biotin carboxyl carrier
                     protein"
                     /protein_id="HEX9917017.1"
                     /translation="MNERKIRKLIKLVEESNIGELEVSSWGRMVRIRKKVAEAENSAT
                     AAIPTITVSPPVNNPAPQVAPEPTAETKKEEIPANLVPIKSPMVGTFYRAPAPGAKLY
                     TDVGQIITVGQVVCIIEAMKLMNEIESEISGKVVKILVENAKPVEFGQTLFLIEPA"
     gene            8635..9759
                     /locus_tag="VGB16_04700"
     CDS             8635..9759
                     /locus_tag="VGB16_04700"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_012471190.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_process: GO:0043107 - type IV pilus-dependent motility
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="type IV pilus twitching motility protein PilT"
                     /protein_id="HEX9917018.1"
                     /translation="MAMSLKKMLAEVVNAKASDLHLRVGLRPTLRIDGVLKPIDPDPL
                     TSEDVSKIMQQMLNEQQLERYHQQSELDIALSVSKLGRFRINFYRQRGTSGIAIRAVN
                     TVIPSFEELNLPPVLKNLADSRRGLIIVTGTTGSGKSTTLAAMINYINNVRTDNVLTI
                     EDPIEYVYRDKKSIISQREIGGDTESFATALRHAFRQDPDVILIGEIRDKETMSIALT
                     AADTGHLVLTTLHTLNAVETISRIISFFPPHQHLQIRLLLASTLKSIVCQRLLPRVDG
                     PGRVPAVEILVSTGAVREYLVDPMKNSLIPELIESGNIQYGMQTFDQSIMQLYRNGII
                     SYKEALAQTTNPDDFDLRVRGIVSTSDKGWTEFEGKDSKK"
     gene            9763..>10879
                     /gene="accC"
                     /locus_tag="VGB16_04705"
     CDS             9763..>10879
                     /gene="accC"
                     /locus_tag="VGB16_04705"
                     /EC_number="6.3.4.14"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002605464.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0009317 - acetyl-CoA carboxylase complex
                     [Evidence IEA];
                     GO_function: GO:0003989 - acetyl-CoA carboxylase activity
                     [Evidence IEA];
                     GO_function: GO:0004075 - biotin carboxylase activity
                     [Evidence IEA];
                     GO_process: GO:0006633 - fatty acid biosynthetic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="acetyl-CoA carboxylase biotin carboxylase
                     subunit"
                     /protein_id="HEX9917019.1"
                     /translation="MYKKILIANRGEIALRIIRACKELGIRTVAVYSEADRESLHVRF
                     ADEDVCIGPSSSAQSYLDPKRIISAAEVTNSDAIHPGYGFLAENAGFAEICESCGVKF
                     IGPSSLMIKMMGDKALAKKSMKDAGVPVIPGSEGVVQSWEEGLEIAKEIGFPVMVKAA
                     AGGGGKGMRIAKDEEELKRNFSLAQVEAKAAFNNQEVYIEKVILNTRHIEFQLLGDSF
                     GNVVHLGERDCSLQRRHQKLLEESPSVAVDEDLRQRMGEAAVKGAKNVGYESAGTIEF
                     LLNKDKDFYFMEMNTRIQVEHPVTEEVTDVDLIKEQIKISYGEKLPFTQDDIKVKGHA
                     MECRINAEDPEKDFMPSPGLIKSFHVPGGHGIRVDTHA"
BASE COUNT         3198 a         2162 c         2368 g         3151 t
ORIGIN      
        1 ccattcgaag ctatttaaga gccggatatg tcgcccaaca attatctagt ccgaatgtac
       61 agcaaatcca gacatactgc ccgcatctaa aaccagggca atgataccat tccttccagc
      121 aataagtacc gtcccttgca tcgactggtg cagttgcaaa aatctgagca acaaaagtta
      181 cggctaaaac tacgaccaga agaataagaa tcttcttctt catttacctc acccccattc
      241 tttttatttt aagtgaccat ttgtctgaac ttaataaata agcaaaattc ttgccgaatt
      301 aattaagggt agggttttta atgtatgacg cagtccgttt attgacagcc acttaagact
      361 gctcattaat tgcacatttt cgactgaagg attttttagg tataggtatg caacaaattg
      421 cggcccatgt ggtcgatttt gcggttttat ctaactaaca tgttgttatc acacaactta
      481 gacgattcgc caaaattggc gggtaaaaac agtcttttgc ggacttttta agtcctcatt
      541 tattcttttt cctcaattta ttatctttaa acagttcatc tatagcctta gaaatattct
      601 cttgagtgtc ttttaaatcc tcgataaata ttacttttcc atcagaggta acgattatgc
      661 ttttagggtc aacttccaaa tcataatttt tgtaataact atccgattct tccggctcga
      721 cttttctaac accaacgtcg taagacagct tagctttttg aacgtaatct cggattatac
      781 ttagcggatt atcttttcca tagacaagaa caactcttaa tcccttaggg ccgtattcct
      841 cataagctga ctgcatcaaa agcaatcgtt tgtgttccct gcaagtaggg cagttggccg
      901 aaaacagagt cagaagtatt ggctttccaa caaaatccgc aagctttttg atactattat
      961 ccttaacatc ataatattcc agagaaggta gattctgccc cttaacaaat tcatcttcac
     1021 ccaaaagctt ctcttcgttt aagacaaatc tgtccacggt cattctcaca agtatattgg
     1081 gcagattgta atccctgaac ttcactattc catctctatc gattatgaat gcccgaaagc
     1141 cctcgatttc cttgttacaa tactttttgg taatctcgcc atctttatct tttaacacta
     1201 aaatcggaga ataaacttta gccacatctt tgacagcaag actatctttg gcagtggaga
     1261 taatccacac cacaaaacct ttatctctgt atttccccaa aaggagctta taatagtaag
     1321 cttccaaaaa agatttttca tcctttatct cccggaatat caagagagtt atttttccct
     1381 ttaactcatc tgtgctgtgt gaaatcccct cgaggtcctt gagaacaaaa gcaggggcaa
     1441 cctcctcgtt ttttattccg ggtaattcag cttgtggctt atagggtatt ggctttgtca
     1501 tgcttccatg acgaaggaaa agatagacat tcaaaacgag gaatatggca attagcgcta
     1561 acaaaaatga atttctaatt ttcattctac gctccgctgg tttaaaattt gctcatagac
     1621 tggatgaaaa ttcaacacaa ataatactca gtatattaat agtatcgcca gatagtaaga
     1681 ttaatttagg aaacacgaga ttagaataga tacagaccat tgcttcatct gtgtctatca
     1741 tagtgggcta tttagcggga tattccacat tagcccacca ccttagtttc agctccggga
     1801 aaaccttatc cctcttttcg cagtccccta aaaatgtcca ttccccctca tctaatttct
     1861 cgcccctagc atatttttct gccattgagg caagtctaac aaaattgttg tgatgctcgg
     1921 atagtctcaa ttcagcataa tctctggcgc tccaggtgga aatcaaaaac tgccaatccg
     1981 atgcttccat aagcaatagt tcccgggcaa gctgtttcag gattttattt aagttctcat
     2041 cgcccgtctc tttgaacttt tcagccaagg tttgcatcca gagctcatcc tcatagatat
     2101 gtttccaggt ccattcggtc cacttgttaa gccagatata atgatatccc ccttctcccc
     2161 aggagccttc ggggatggaa ataactttgc tcggcttaga ttcgtccaaa aacttgctgc
     2221 aggtagtaag ctcgatttcc ggatcgtctg caatattctc cagaaccaac tttagaaatt
     2281 ttgtcccttc aaaccaccaa tgtccaaata actcggagtc ataaggggcg cagacaatcc
     2341 ccttttgttt agttttctga taatattcaa ccaacaatct tttaaccaat cccttgaaat
     2401 gggtggcatt ctccggtacc attctatccg catccggtgg ataatattcc atcttatccg
     2461 ccaaatcgct tttggcgctg gtgattttcc aataacgatg tccacccgga aagtgcttct
     2521 tgtgaaagtc aagatagttt ccatctccgg gatatcccca ttcaccgctc cacacttgta
     2581 aacctgtgtc cgggtcgcgg gtgaatatcg ccactggctt tttcccctca ccgccggagt
     2641 tgaccagata aacttctctt ggggtcttgt cgaattcttc ctctctgggc tgatactcct
     2701 tttcgaatcg tccccagagc tgttttagcg cttcaaacct gtcaacataa actccgatcg
     2761 ctttcccgcc tcttagcaac gcagaatcga cgataaaata atctatccca ttttcactta
     2821 aaaactcatc cactcctttg cgcagatgat ttggctttcc aattgaagtt tcaaccggag
     2881 aagtccagtt gtatcttggt ctataagcgc attccggcaa ccagattccc ctcgattctt
     2941 tgccaaagtg tttctcgtaa acctgcgctc ctaattttat ctgagcctgc acgctggtat
     3001 cctcagataa taaaggcaaa tacccatgtg tggctgcaga ggtgatgatt tcaatatggt
     3061 cactatcctg taatgttttg aaagccgcaa tcaggtctct cttatattta ttctcgaaat
     3121 catcgttaat cttgctgtaa tactctgtcc acttcaaagc taatttcaaa aggttttcct
     3181 tcttatacct ttggaattcc actacgtcat cttcagctgc ctttatcttt tgttccaaat
     3241 aatctgataa ttcctctttg aaagttgggt cagccagctg ttccaacaat accggggtta
     3301 tcccgattgt gagtttagga gaatatccct cctccaccag ttcattcaat atattcaaaa
     3361 ggggaatata agtctcagcc gaggcttcat tcagccagtc cataccatgt ggccacttac
     3421 cgtgagcgat aacgtaaggc aaatgagaat gcaatacaaa agcgaaagag ccaatagcca
     3481 ttttggttcc ttttttataa aattgttata gcaatataat ttattatcgg gtggagacaa
     3541 gctccacccc tacgcggtta ttcattggtt cgcgtagggg cgaagttcat cttcgcccga
     3601 cctcttaagg gtatttatac ttattttaat aagttcttcc ttatttatac ttctgtcgag
     3661 aacagttccg agcactttgt tttgcagttc tatactcaaa tcttccaaac ccgccattat
     3721 gccaatcaac ttcttcgtta gagaattgat tttatcatta aactcttttt gtttttcttt
     3781 taggggaatt ttgttatctg gtgaacaatt tatcggatag aatgcaaaga aatcggactc
     3841 attgtccgca aaagacaagt aagcatctcc tatttttaag gtagtttctc ttaattcggg
     3901 aaaaacaatc tgattgaaat cataggtaaa agaaaaattg cgtagtttgg atccacttaa
     3961 ctccattagt tctaataact tggtgtgaac tgtttcccaa tcgcaagacg ggcatacaaa
     4021 aggcgaagtt gttttatgcg ttaattctat atcgctgcgc aaaattgcta gataagtgta
     4081 gaaatcaatc aatgattcat taaaaggtct gatagtgcgc tgtagttcct tgtaagcttc
     4141 gtattccact tgtcttgcta gtagcttatc ttggagcttt agattttttt catgtgtttt
     4201 tttgacctca tttttcataa taataaaagc gataatccag cctgctattg taagaattgc
     4261 gattataata tctgataaat caattttgtt taaaatggtt tctaagattt tcgtcatcac
     4321 acaattcaat ttattgggtg gagacaagct ccacccctac gcgaccattt tcttgtagaa
     4381 acaaatcagc tcgcgtaggg gcgaagttca tcttcgcccg agctcgaatt ttaaagggtt
     4441 gttcgttata tactccatct tttctctata atctttttcg tttctgataa tatggtcata
     4501 aaatccccgt tgccaaatct tgtcataaat tcctactctt tctctaactt ctttagcaat
     4561 tagagattta aaagattgga ttacttttga taagtttgta ttatttctat ttttaattgt
     4621 tatcattaga tggagatggt tttccattat agcacatccc tcaatagtta gagaaaaagc
     4681 accattcaat ttttcgatat tattcttaat aatatctcta attccatttt tggcaaagca
     4741 ttttctgttt tccctgcaat ttatggtcac aaaataacaa ccttcggagg aataatcaaa
     4801 accttttaag cgtagagagc ttcttcgttt tggtaatttt gtcatacatt ttcgggcgga
     4861 cgtaaagtcc gcccctatgc ggttttgtta ggcggggaca agctctgccc cgacgccctg
     4921 caccatatag tgcagggtca cgcattttat tcttttttct gaaaccctat ctgcatatcc
     4981 tctttgtcgg gaaggttaat cttcccgaat tcgttttcaa aattcttgat gctttcctcc
     5041 agattcttta aaaacatctt ggcgtgctgg ggagtcatta tgattcgggc atagagcttg
     5101 gtttttggca ctccgggcat tattcgggcg aagtcgaata taatctctga aggcgagtgg
     5161 acaatcaaga ccaaatttga atatatccct tcggattcct tttcacctaa ttcaatgttt
     5221 aactttttct tttgctccac ttttttctcc ttgaagacag tttaaaagtt tacaagttta
     5281 ccctgcaccg tacagtgcag agtttaagct ttaaaaattc agaaaattgt aattatccta
     5341 acagataagg tgaaaatcat ccttgcgttt tgactgcatt ttaatatatt agtgaataat
     5401 gtcaaggtaa ttttagaaag ataaacacta tgcgtaaaat caaacttgct cgcttagaaa
     5461 agattttttc aaatctgggc gggcataaga tattagtggt tggtgatgtg atgctggatg
     5521 aatatctgtg ggggaatgtc tcccgcatct caccagaggc accggttccg gtggtggagg
     5581 tcacaagaga gacaatcaaa ctcggcggtg cggccaatgt tgccttaaat atcgaaacct
     5641 taggagctga gccgattttg gtagggctgg tggggaagga caaaaatggt gagaagctga
     5701 ggagtttact taagagttta agaatatcag atgaaggaat actcgtggac aagaatcgtc
     5761 ccactacctc caagaccaga ataatcgccc atcatcagca agttgtgcgt gcggacagag
     5821 aaaatataga ggagatgaac agcgagactt gcaagaggat tcttaggttt gcgaatgata
     5881 agatgaagga gatagatgcc ataatcatct cggattatgg taaggggatg attaccttga
     5941 atctattgaa tgagttgatt tttttagcaa ggaaagatag aaagtttatc gcagttgatc
     6001 ccaacaaaga gacacatttc ataaattata agaaagtgtc cataattact cccaatcttg
     6061 atgaagccag ctttgcattc gggaaaagaa tcaaagatga gaaaagcttg gaagaggttg
     6121 gctggggact tctggagcgc ctggaggtgg aatcgatact tgtcacccga ggggagaaag
     6181 gcatgtcttt atttcaaaga gataaaactt taacccattt tccaaccgta gccaagaaag
     6241 tatatgatgt aaccggagca ggagatacgg tcatctccgc tttcagcgta gcctattgtg
     6301 ctggtgcgga tttgaaggag tcagctctga tttcaaatca tgcggctgga atcgtagtgg
     6361 gagagatcgg aacaggccaa gtggataaga agaccctgtt ggaggatttg aaagattttg
     6421 tgaatggaaa tgaggactaa aatgggtcaa gttgtgaagc tgaatagatt gattagaatt
     6481 agggaaaaag ccagaaaagc ccgaaagagg attgttttta ctaatggctg tttcgatatc
     6541 ttgcatcgcg ggcatataga tttcttaaaa aaggcaaaaa gattagggga tattttgatt
     6601 gttggcttaa ataccgattc gtcggtcaaa aagataaagg gaaaacagag gccgataatt
     6661 agccaaaatg atcgagcgga aattttagct aaccttttgc cggtggatta cgtttgccta
     6721 tttcatgaag agacgccgag gaggcttatc tctgctttga tgccggatgt attagttaaa
     6781 ggaagggatt ataagaagag agggatagtt ggaaaagata tagttgaatt gagcggggga
     6841 aaagtagtca gaataaaatt ggtacagggg aaatccacta agcatataat caatacagtt
     6901 ctcaatagat ataagagata gaattcagat aacacaacat ttttgcttga caccttgaag
     6961 taaaggtgtt attgtttttt attgggtaaa atgaaaaacc gcataagaag aatagtttcc
     7021 cttttagaaa aagaaaacct ggatgccctt ttggtcaatt tccagccaaa tgtgagatac
     7081 ctaagcgggt atgatggcac aaatggtttg gtcgtgctcg cttctgataa gagactcttc
     7141 attaccgact ttcgttatca ggagcaagcc caaaaagagg tcaaagattt aaagctgcat
     7201 tttgcccaga gggagctgat ttatgattta gctaaattca aattcctgca aaaaaggaat
     7261 caaaggatag gatttgaagc caatcatact agctacagca cagttcagaa gctaaaagag
     7321 attattccta atgcgctctt ggtgccaact gaaaatctgg tggaatccct tttgataata
     7381 aaggataaag gcgaagtggc caaaatagag aaggcagccc agataactga caaaactttt
     7441 tctcatattc ttgatctggt caagccggga gtaagagaaa aggatttggc agcggagata
     7501 gagcatagtt ttaagaggat gggaggggat ggagctgctt tttccaccat tgttgcttca
     7561 ggatataggt ccagtatgcc gcacggtagg gcatcggaaa agaaactgaa agtcggggaa
     7621 tttgtcacct ttgatatggg ggcaatctat caaggatatg tctcggatat gactcgcacc
     7681 ttggttttgg gaaaggcaac acttaagcag aggaagatat acagtttagt actgtcggca
     7741 cagacaaaag ccatatccag agccaaagca ggaataagtg gaatggaatt agataaggtt
     7801 gcccgcgagg tcatcgccaa ggcaggatat ggtaagtatt ttgggcatgg tctggggcac
     7861 gggttgggtc tggaggttca ttctggacca atggtcaatg ctcgaagcaa gactatcttg
     7921 aagcccggca tggtcatcac catcgagccg ggaatatata ttccagggtg gggtggagta
     7981 aggattgagg atgatgtttt aatcactgca cggggatgca aggtactaaa ccaatcccct
     8041 aaaaatctca tagaaatcag gtgaggtaaa aatgaacgag agaaaaatca gaaagttgat
     8101 aaaattagta gaggagagca atatcggcga gcttgaggtg tcgagctggg gaaggatggt
     8161 gaggattaga aagaaagtag cggaagcgga aaattcagct accgccgcta ttcccaccat
     8221 aactgtgagc ccaccagtca acaatccggc tccgcaagtt gctcctgagc ccacagcgga
     8281 aacaaaaaag gaggagattc cggctaatct ggtgccgatt aaatctccca tggtagggac
     8341 attctaccgt gcacccgcac ctggggcaaa actatacaca gatgtaggtc aaatcatcac
     8401 tgttggccag gtggtctgca ttatcgaagc aatgaaattg atgaatgaga tagagtcaga
     8461 gatttcgggg aaggtagtaa agattcttgt agagaatgcc aaaccggttg aattcggtca
     8521 aaccttgttt ttaattgagc cggcttaaga ataatttcta aacttagctc caaaaaagac
     8581 gaaataaagt aatagataga gtttcaaggc cttgatttcc aaagaggtta agctatggcg
     8641 atgagcttaa aaaagatgct tgccgaggtg gttaacgcca aagcctcgga tttacatctg
     8701 agagttggct taaggcccac tttgcgcata gatggcgtgc tcaaaccaat tgaccctgat
     8761 cccttaacct ctgaagatgt aagcaagatt atgcagcaga tgctgaatga gcaacaattg
     8821 gaacgttacc atcagcagtc tgaactggat atcgccttga gcgtgagcaa attaggacgt
     8881 ttccgcataa acttttatag acagcgagga actagtggca ttgccattcg cgcagttaat
     8941 accgtaatcc ccagttttga ggaattgaat ctcccaccag ttttaaagaa tctggctgat
     9001 agccgcaggg gtcttatcat tgttaccgga actaccggaa gcgggaaatc gaccaccttg
     9061 gcggcaatga taaattatat taacaatgtc cgcactgata acgtcttaac catagaagac
     9121 cccattgaat atgtctatcg ggacaagaaa agcatcatca gtcaaaggga gattggaggg
     9181 gatactgagt catttgccac tgctttgcgg catgccttca gacaggaccc ggatgtcatc
     9241 ctgattggtg aaataagaga taaggagact atgtccatag ccctgactgc agcggatacc
     9301 gggcatttgg tcctaactac cctgcatacc ctaaatgcgg ttgagaccat aagtcgaatc
     9361 atctcttttt tcccgcccca tcagcatttg cagataagat tacttctcgc cagcaccctg
     9421 aaatcaatag tctgccagag gcttttacct cgagtggatg ggccaggaag ggtgccggca
     9481 gttgaaatat tggtttctac tggtgcggtg agggaatatc tggttgaccc gatgaagaat
     9541 tcccttatcc cggagcttat cgaaagtgga aatatccaat atggaatgca gaccttcgac
     9601 cagtcaatta tgcagcttta ccgcaatgga atcatatcct acaaagaagc cttagcccaa
     9661 accacaaatc cggatgattt cgatttacgg gttaggggta tcgttagtac ctccgataaa
     9721 gggtggacgg agtttgaggg gaaagatagc aagaagtaat ttatgtacaa aaaaatattg
     9781 attgccaatc gaggggaaat tgccctgagg ataatccgtg cgtgcaaaga gctgggtatt
     9841 cgaacagtag cagtctattc cgaggcagat agggaatcgc tccatgttcg gttcgcagat
     9901 gaggatgtct gcatcggtcc cagcagttca gctcagagtt accttgaccc caaaaggata
     9961 ataagtgcgg cagaggtgac aaattccgat gccattcatc cgggatacgg attcttggcg
    10021 gagaatgcgg gttttgcaga gatctgtgaa tcttgcggag taaagtttat tggaccttcc
    10081 tccttaatga taaaaatgat gggagacaag gcattagcca agaaaagtat gaaagatgcc
    10141 ggggttccgg taatcccggg gagtgaaggg gtggtgcaat cctgggaaga aggtttggag
    10201 attgccaaag agataggttt tccggtgatg gttaaggcgg ctgcaggtgg tggtgggaaa
    10261 gggatgagga ttgcaaagga cgaagaggaa ttaaaaagaa acttcagttt agctcaggtg
    10321 gaggccaaag cggcttttaa taatcaagaa gtctatattg agaaagtgat tctaaatacc
    10381 cggcacattg agtttcagct tttgggtgat tctttcggca atgtggttca tctgggagaa
    10441 agggactgtt ctcttcagag gagacatcag aagcttttgg aggaatcacc ttcagtcgca
    10501 gtagatgaag atttaaggca acggatgggt gaggctgcgg tcaaaggagc gaaaaatgtt
    10561 ggatatgaaa gtgctggcac catagagttt ctgttaaata aggacaagga tttttatttt
    10621 atggagatga acacccgaat tcaggtggag catccggtaa ctgaagaggt tacagatgta
    10681 gatttgataa aggagcagat aaagatttcc tacggggaga agttgccttt tacccaggat
    10741 gatataaaag tgaaaggaca cgctatggaa tgccgcatca atgcggaaga cccggaaaaa
    10801 gatttcatgc ccagccccgg cttgatcaaa agttttcacg taccaggcgg gcatggtatc
    10861 agagtggata cgcatgctt
//
LOCUS       DASWLN010000140        14364 bp    DNA     linear   ENV 20-FEB-2024
DEFINITION  MAG TPA_asm: Candidatus Zixiibacteriota bacterium isolate
            SMAG_U2949 k141_1004146, whole genome shotgun sequence.
ACCESSION   DASWLN010000140 DASWLN010000000
VERSION     DASWLN010000140.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37960594
            Sequence Read Archive: SRR10376091
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Candidatus Zixiibacteriota bacterium (soil metagenome)
  ORGANISM  Candidatus Zixiibacteriota bacterium
            Bacteria.
REFERENCE   1  (bases 1 to 14364)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 14364)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (26-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/12/2024 08:28:26
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 1,440
            CDSs (total)                      :: 1,416
            Genes (coding)                    :: 1,412
            CDSs (with protein)               :: 1,412
            Genes (RNA)                       :: 24
            rRNAs                             :: 1 (16S)
            partial rRNAs                     :: 1 (16S)
            tRNAs                             :: 21
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 4
            CDSs (without protein)            :: 4
            Pseudo Genes (ambiguous residues) :: 0 of 4
            Pseudo Genes (frameshifted)       :: 0 of 4
            Pseudo Genes (incomplete)         :: 3 of 4
            Pseudo Genes (internal stop)      :: 1 of 4
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..14364
                     /organism="Candidatus Zixiibacteriota bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_1004146"
                     /isolate="SMAG_U2949"
                     /isolation_source="soil"
                     /db_xref="taxon:2053527"
                     /environmental_sample
                     /country="USA: Oregon"
                     /lat_lon="42.99 N 123.63 W"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            <1..746
                     /locus_tag="VGB16_04710"
     CDS             <1..746
                     /locus_tag="VGB16_04710"
                     /EC_number="6.3.2.6"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_013580779.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004639 -
                     phosphoribosylaminoimidazolesuccinocarboxamide synthase
                     activity [Evidence IEA];
                     GO_process: GO:0006164 - purine nucleotide biosynthetic
                     process [Evidence IEA]"
                     /codon_start=3
                     /transl_table=11
                     /product="phosphoribosylaminoimidazolesuccinocarboxamide
                     synthase"
                     /protein_id="HEX9917020.1"
                     /translation="LTQMSLFWFDFVEDIIPNHLITSEVHKFPKELQKSRDQLEGRSM
                     LVKKAQRMDVECVARGYISGSLWKEYKEIMWSAEEGKDVKIHGIPFPYGLKESEKLPN
                     PIFTPATKEKTGHDENISFKKTEEILGRELAGFLKEKSIAIYKKARDYAEERGIIIAD
                     TKFEFGILNDKIILIDEILSPDSSRFWSKSNYKVGKPQDSFDKQYVRDYLESINWNKK
                     PPAPELPEVIIENTLKKYQQAYQALISKK"
     gene            755..2341
                     /gene="purH"
                     /locus_tag="VGB16_04715"
     CDS             755..2341
                     /gene="purH"
                     /locus_tag="VGB16_04715"
                     /EC_number="2.1.2.3"
                     /EC_number="3.5.4.10"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_012470802.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003824 - catalytic activity [Evidence
                     IEA];
                     GO_function: GO:0003937 - IMP cyclohydrolase activity
                     [Evidence IEA];
                     GO_function: GO:0004643 -
                     phosphoribosylaminoimidazolecarboxamide formyltransferase
                     activity [Evidence IEA];
                     GO_process: GO:0006164 - purine nucleotide biosynthetic
                     process [Evidence IEA];
                     GO_process: GO:0006189 - 'de novo' IMP biosynthetic
                     process [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="bifunctional
                     phosphoribosylaminoimidazolecarboxamide
                     formyltransferase/IMP cyclohydrolase"
                     /protein_id="HEX9917021.1"
                     /translation="MENLEQEGLVKISRALISVSDKTGLKELASVLTDFGVEIISTGG
                     TERELKKSGIKTVSLSSFTGFPEILDGRVKTLHPKIFGGILAKREDEEHKKTVIQQEL
                     KLIDLVVVNLYPFEKTISQPNVKIEEAIEQVDIGGPSLLRAAAKNYKDVAVLTSPHRY
                     EEIIKELKEKDGCLSLVTRESLALEVFQHTSSYDNVIAGYFNREVVKSEDIFPANLIS
                     SYRKVHSLRYGENPHQKAAYYRGEGFPGPSVITSEILSGKEISFNNLVDLDAALIMIE
                     DFTRPFAVILKHTNPCGAACADNLADAYSDALACDPISAFGCIIGLNKIVDMETAKKI
                     DETFFVECVVAPGYKEEALELLKKKKNRRFLACPQLLKDRKGEPNQIKIIKGGALVQT
                     PDNLETTARDLKVVTEVSPTPEQIKSLLFGWKVVKHVKSNAIILAQGERTVGIGAGQM
                     SRVDSSIIAVRKAGERAKGSVLASDAFFPMRDGVDAAAEAGVVAIIQPGGSKGDEEVI
                     KAANEHKIAMVFTGVRHFKH"
     gene            2610..3833
                     /gene="coaBC"
                     /locus_tag="VGB16_04720"
     CDS             2610..3833
                     /gene="coaBC"
                     /locus_tag="VGB16_04720"
                     /EC_number="4.1.1.36"
                     /EC_number="6.3.2.5"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_007286542.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004632 - phosphopantothenate--cysteine
                     ligase activity [Evidence IEA];
                     GO_function: GO:0004633 - phosphopantothenoylcysteine
                     decarboxylase activity [Evidence IEA];
                     GO_process: GO:0015937 - coenzyme A biosynthetic process
                     [Evidence IEA];
                     GO_process: GO:0015939 - pantothenate metabolic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="bifunctional phosphopantothenoylcysteine
                     decarboxylase/phosphopantothenate--cysteine ligase CoaBC"
                     /protein_id="HEX9917022.1"
                     /translation="MLKDKNIILGITGGIAAYKSAEICRRLKKLEANVVVVMTKAGAK
                     FITPLTMETLSENEVVTELFPENKVVKTRHIDLATWADLVLIAPATANIIGKIASGIA
                     DDILSTVVMSTLSPVLLCPSMNENMYLNPIVQKNIQKLKDLGYKFIEPGIGDLACGTV
                     GKGRLADLDIILREVADILVKKKDLEGKKILITAGPTQEPVDKVRFLSNRSSGKMGYA
                     LAQEAKARGAKTILISGPTGLSKPAGMRFHQVKTAGEMYCQVKLYLKEADILIMAAAV
                     SDFAPKIVQSQKMKREGKEISLELEPTIDILKEVGKNKKGKLLVGFALETENEVENAK
                     GKLKEKDLDLIVVNNPYVSGAGFEVDTNVVTLIDKKGKIEKLPLMPKKIVAEKILDKV
                     VSLLKKPSKKKNERK"
     gene            3820..4530
                     /locus_tag="VGB16_04725"
     CDS             3820..4530
                     /locus_tag="VGB16_04725"
                     /EC_number="3.2.2.27"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_020402968.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004844 - uracil DNA N-glycosylase
                     activity [Evidence IEA];
                     GO_function: GO:0046872 - metal ion binding [Evidence
                     IEA];
                     GO_function: GO:0051539 - 4 iron, 4 sulfur cluster binding
                     [Evidence IEA];
                     GO_process: GO:0006281 - DNA repair [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="uracil-DNA glycosylase"
                     /protein_id="HEX9917023.1"
                     /translation="MNENKKKFLDLVDLTEKYVKQQIDLGFDELHKERKPETKSEVEP
                     ETQQITLKEFHEKIKNCTECHLHKARTNFVFGTGKDDADIMFIGEAPGREEDLKGEPF
                     VGEAGKLLNKILAAVNFKREEVYIGNVLKCRPPENREPLPEEIETCEPYLLKQIAIIK
                     PKIICALGRISAQALLKTKLPLNKLRGRFHDYHGRKLLVTYHPAALLRYPQYKKDTWE
                     DIQLLRKEYDKMISPQDL"
     gene            4543..5916
                     /gene="dnaB"
                     /locus_tag="VGB16_04730"
     CDS             4543..5916
                     /gene="dnaB"
                     /locus_tag="VGB16_04730"
                     /EC_number="3.6.4.12"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_015328187.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003677 - DNA binding [Evidence IEA];
                     GO_function: GO:0003678 - DNA helicase activity [Evidence
                     IEA];
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_process: GO:0006260 - DNA replication [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="replicative DNA helicase"
                     /protein_id="HEX9917024.1"
                     /translation="MNQKETSGERVPPQAIEVEQAVLGTILLDREAIGRAIEIIDEEC
                     FYREAHSKIYSAIVSLYDRNEAVDIVTATEELTKRGQLEEVGGRIYLINLTESVATAA
                     NIEYHARIILEKATLRRLISASSKIVSQCYDTTQEVDELLDKAEQEVFSISEGRIKEG
                     FVPIGEILPRTFEAIDEYAKRGGIVTGIATGFTELDVLTGGLQNSDLVVIAGRPSMGK
                     TSLALSIAEHVAVDEKVPVAIFSLEMTKSQLAQRMLCSRARFSTHKMRTGRISDHEYG
                     NLAIAVGPLSEAKIFLDDTPSLGILQMRAKARRLKAKENIGLVIVDYLQLMQGPRAIE
                     SRQQEISLISRSLKGLAKELDVPVVALSQLSRAVEIRGGERRPQLADLRESGAIEQDA
                     DLVMFVYRPEMYNINQFRDGRSTEGIAEIIVSKHRNGPTGEILLTFIKDYARFENPEL
                     VHTETPS"
     gene            6013..6723
                     /locus_tag="VGB16_04735"
     CDS             6013..6723
                     /locus_tag="VGB16_04735"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_006929602.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0042626 - ATPase-coupled transmembrane
                     transporter activity [Evidence IEA];
                     GO_function: GO:0140359 - ABC-type transporter activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="ABC transporter permease"
                     /protein_id="HEX9917025.1"
                     /translation="MAILIKNLLINLPSALTHVSLVTEQMLIMGVNSLPLVVFTSIFT
                     GGVSAVQAAYQFSDYIPMRYLGTAVGKAVVIELGPVLTALVVVGRVCASITAELGTMK
                     VTEQIDAMETLALDPVKYLVVPRFVAGFVMLPVLVLFSDFVAILGGLAVSVFFLGISS
                     YTFLNGLKMFFHLSDVFGGLLKAFVFGSIIAIVGCYQGFRTEGGAEGVGRSTTRAVVL
                     ASVLVLISDYIIASLLFG"
     gene            6716..7486
                     /locus_tag="VGB16_04740"
     CDS             6716..7486
                     /locus_tag="VGB16_04740"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_013257036.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_function: GO:0016887 - ATP hydrolysis activity
                     [Evidence IEA];
                     GO_function: GO:0042626 - ATPase-coupled transmembrane
                     transporter activity [Evidence IEA];
                     GO_function: GO:0140359 - ABC-type transporter activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="ABC transporter ATP-binding protein"
                     /protein_id="HEX9917026.1"
                     /translation="MDKKRKAMIEINDLYKGFHNKPVLEGVNLAIQKGETVVIIGRSG
                     CGKSVLLKHIIGLLKPDKGQLLIDGEDITAYPKDKLNQLRQRFGMLFQGAALFDSMTV
                     AENVGLGLSEHTEMTEGQIREIAAEKLKMVGLVGVEDLKPAELSGGMKKRVGLARAIA
                     MNPEFILYDEPTTGLDPIMADAINDLVINLRNTLKITSIAVTHDMASAYKIADRIAML
                     YKGKIIFAGTPEETKNSDNPVVQQFIQGRSQGPIPTLE"
     gene            7488..8888
                     /gene="radA"
                     /locus_tag="VGB16_04745"
     CDS             7488..8888
                     /gene="radA"
                     /locus_tag="VGB16_04745"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_012545683.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_process: GO:0006281 - DNA repair [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="DNA repair protein RadA"
                     /protein_id="HEX9917027.1"
                     /translation="MAISDKIKTIYICQVCGYKSPRWLGKCPECGTWDSLIEEKITPT
                     KRWEEPGGQLEMPIPVTKITKDKEERIITGAREFDRVMGGGIVNGSVTLVGGDPGIGK
                     STLLLQISESVALKNKKVLYVSGEESAKQVKIRAERLGVLSDNLEVLAENNLHYILAV
                     LSQRKFDVVIIDSIQTSYTQELESAPGSVSQIRECAYRFINYAKTSGSSFFLIGHVTK
                     EGAIAGPKVLEHMVDTVLYFEGEKSHTYRLLRSVKNRFGSTNELGIFQMMAKGLVEVE
                     NPSRAFLSERRKDSPGSVVICSIEGTRPLLLELQALVTPSTFGMPQRVCSGVDYKRVS
                     LLLAVLEKMGGISLSKYDVFVNLAGGVRIEEPALDLGIITAIASSYRDMPIDPEAVVI
                     GEVGLSGEIRTVDQIERRIKEAEKLGFKRCILSQGNLKEIENKFSIQVIGTREINETF
                     RKLFIQKARRSETEDK"
     gene            8869..9168
                     /locus_tag="VGB16_04750"
     CDS             8869..9168
                     /locus_tag="VGB16_04750"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEX9917028.1"
                     /translation="MRLKISKALRGGEYLVSFKTLGFSPEEEEKAGRFGSPEIDFSLD
                     GLGTRKLEGLDISVKCESPEKAEEFILQTKDKIKERLTQLKDMVDTFSGEEELSL"
     gene            9171..9740
                     /locus_tag="VGB16_04755"
     CDS             9171..9740
                     /locus_tag="VGB16_04755"
                     /EC_number="1.5.1.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003023148.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0010181 - FMN binding [Evidence IEA];
                     GO_function: GO:0016646 - oxidoreductase activity, acting
                     on the CH-NH group of donors, NAD or NADP as acceptor
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="flavin reductase family protein"
                     /protein_id="HEX9917029.1"
                     /translation="MAKKQLPPQALLIPLPVVMVSCQSKGGPNIITLAWAGVVCSEPP
                     MVGISIRPSRYSFGIIRDSKEFVLNVVNEDLIRQADFCGSHSGRDVDKFKELNLTPIK
                     GEKVKSPLIKESPINLECQVKDIIELGTHHLFLGEIVATHIDERYLDSNGNPDIAKIR
                     PAVYCTKAHQYWGGLSKVLGTYGYTAKKR"
     gene            9812..10969
                     /gene="tgt"
                     /locus_tag="VGB16_04760"
     CDS             9812..10969
                     /gene="tgt"
                     /locus_tag="VGB16_04760"
                     /EC_number="2.4.2.29"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_020252234.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0005737 - cytoplasm [Evidence IEA];
                     GO_function: GO:0008479 - tRNA-guanosine(34) queuine
                     transglycosylase activity [Evidence IEA];
                     GO_process: GO:0006400 - tRNA modification [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="tRNA guanosine(34) transglycosylase Tgt"
                     /protein_id="HEX9917030.1"
                     /translation="MHSFDFKLEAKDRASKARAGIINTPHGQINTPVFMPVGTQASVK
                     TLCSEELDNLGAEIILGNSFHLYLRPGIEVISQAKGLHQFMSWHKSILTDSGGYQVFS
                     LNSLNKVSEDGVAFKSYLDGSIHNFTPEKIIEIQMALGSDIIMPLDVCAPYPCSGEYA
                     KQSSDLTLKWAKRSKDRLVSFREDKTLPALFGIIQGSTYSDLRLESVKKTVELDFDGY
                     AIGGLSVGEPKNSMWEIVEVVSNHLPSDKPHYLMGVGTPLDLVEAISRGMDMFDCVLP
                     TRNARNGTVFTKKGKIILKNAKYQKDFSPLDETCQCLTCKNYSRAYLRHLFMSEEISG
                     LRLATIHSLYFYINLMKEMREAIVLGNFEEWRKGFFASYSENESVVKNGLE"
     gene            11530..11853
                     /gene="yajC"
                     /locus_tag="VGB16_04765"
     CDS             11530..11853
                     /gene="yajC"
                     /locus_tag="VGB16_04765"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_014098891.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0005886 - plasma membrane [Evidence IEA];
                     GO_function: GO:0015450 - protein-transporting ATPase
                     activity [Evidence IEA];
                     GO_process: GO:0009306 - protein secretion [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="preprotein translocase subunit YajC"
                     /protein_id="HEX9917031.1"
                     /translation="MFETAYAMSSGGGGTPGKGLEFTILWLVLIFGIFYFLIIRPQQK
                     RQKEQQKMISQLQKGDRVVTNSGMFGTIVGINEKENVVVLKIGEDVKVEFLRSSIAGK
                     VAKGS"
     gene            11856..12371
                     /gene="def"
                     /locus_tag="VGB16_04770"
     CDS             11856..12371
                     /gene="def"
                     /locus_tag="VGB16_04770"
                     /EC_number="3.5.1.88"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_008290908.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0042586 - peptide deformylase activity
                     [Evidence IEA];
                     GO_process: GO:0036211 - protein modification process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="peptide deformylase"
                     /protein_id="HEX9917032.1"
                     /translation="MARLPIRLYGDPTLRKRAEKIEEISQEIKNLANDMLETCKEVKA
                     IGLAGNQVGVAKMIFVVDRTSVDLNEKPLVVINPEIVEVEGEDLGEEGCMSVPGAFEQ
                     VKRPHKVKIKGLDLEGKEVTIEGEGLLARVLMHEIDHLNGLLFIDHLSSIRRQLLSKR
                     LREISREGSSE"
     gene            12376..13308
                     /gene="fmt"
                     /locus_tag="VGB16_04775"
     CDS             12376..13308
                     /gene="fmt"
                     /locus_tag="VGB16_04775"
                     /EC_number="2.1.2.9"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_014561004.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0005737 - cytoplasm [Evidence IEA];
                     GO_function: GO:0004479 - methionyl-tRNA formyltransferase
                     activity [Evidence IEA];
                     GO_process: GO:0006431 - methionyl-tRNA aminoacylation
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="methionyl-tRNA formyltransferase"
                     /protein_id="HEX9917033.1"
                     /translation="MHIVFMGTPEFAIPSLEKLLHSRHKVIAVVTGSDKPKGRGQLVC
                     ATPVKEYVSKYKIPALCPTDLKADDFFKRLSSLEPELGVVVAFRILPQSIFDLPSKGT
                     INLHASLLPKYRGAAPINWALIKGENKTGLTTFFLQEKVDTGNIILQKEVDILSNETA
                     GELSHRLAELGASLLLETIDLIENGKFELKRQDDSQVSLAPKITKEMCKIDWSKSALQ
                     IKNLIRGLSPVPAAYSIFKGKILKVYRAEILNEKSPQAKSGEIIISNRMKSLIVKAKD
                     KALELMEIQLEGKKKMKGEEFIKGCRFQACEKLD"
     gene            13324..14079
                     /locus_tag="VGB16_04780"
     CDS             13324..14079
                     /locus_tag="VGB16_04780"
                     /inference="COORDINATES: protein motif:HMM:NF014078.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF116 domain-containing protein"
                     /protein_id="HEX9917034.1"
                     /translation="MGLGKSLFLRLSILSLMVLFILTGSLWFFISPRIYEINPALALI
                     VKLILVLFLLAITGGLFLIVLSSITEKDFLFPHNEKQITLKILFPINLFLGQLFGISK
                     DRIRESFIKVNNSLVKATRKRIRNDRVLILLSQCLQNYDCPNRVTVDWRNCKRCVKCV
                     LSQIIELGEKNNASVAIATGGTLARKVIVDTNPTAIVAVACQRDLTSGIQDVYPIPVY
                     GVLNQRPYGPCVNTQVDFEELKKALQFISDGRS"
     gene            14080..>14364
                     /locus_tag="VGB16_04785"
     CDS             14080..>14364
                     /locus_tag="VGB16_04785"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_008410684.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003676 - nucleic acid binding [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="S1 RNA-binding domain-containing protein"
                     /protein_id="HEX9917035.1"
                     /translation="MQKDIVINVSEYETRLAILEDLKLVELLMERPEAERMVGDIYKG
                     AVKDVLPGMQAAFVDIGIGKNAFLHVSDVGLVEESGTSRKERKIEDVIKQG"
BASE COUNT         4451 a         2602 c         3628 g         3683 t
ORIGIN      
        1 tattaaccca gatgtccctt ttctggtttg atttcgtaga agatataata cccaatcacc
       61 ttatcacctc tgaagttcac aagtttccaa aagaattgca gaaatctaga gaccagttgg
      121 aaggtaggtc gatgctggtt aagaaagccc agaggatgga cgtggaatgc gtggctagag
      181 gatatatttc tgggtctttg tggaaagagt ataaagaaat aatgtggagc gcagaagagg
      241 gaaaagatgt gaagatacac ggaatcccct tcccttatgg attaaaagaa tccgagaaac
      301 tgcccaatcc gatatttact ccagccacca aagagaaaac cgggcacgat gaaaatataa
      361 gtttcaagaa gaccgaggag attttgggga gggagctggc tggctttctg aaagagaaga
      421 gtattgcgat ttacaagaaa gcaagagatt atgcagaaga aagaggtatc ataatcgctg
      481 ataccaaatt cgaatttggc attctgaatg ataaaatcat cttaattgat gagatcctgt
      541 cccctgactc ctcgcgcttc tggtcaaaaa gtaattacaa agttggcaag ccacaagata
      601 gctttgataa gcaatatgtc agggattatc tggaatccat caattggaac aagaagccac
      661 cggcacctga actgccagag gtgataatag aaaatacctt aaagaaatat caacaggcct
      721 accaggctct aatatctaaa aaataggaga ggaaatggag aatttggaac aggaaggatt
      781 agttaaaata agccgagccc taatcagcgt ttcagataaa accggattga aagagttagc
      841 cagtgttttg acagatttcg gagtggaaat catttcaacc ggaggaactg aaagagagct
      901 aaaaaaatca ggaataaaga cagtcagtct atcctctttc accggttttc cagagatttt
      961 ggatggcaga gtcaagacgt tgcatcctaa aattttcggc ggcatattag ccaagagaga
     1021 agatgaagaa cacaagaaga cagtcatcca acaggagtta aagctaattg atttagtagt
     1081 ggttaacctt tatccttttg agaagaccat ctcccaaccc aatgtgaaaa tagaagaagc
     1141 gattgagcag gtcgatattg gtggtcccag tttgcttcgg gcagctgcca aaaactacaa
     1201 agatgtggca gtactaacca gtccgcatag atacgaagag attatcaaag agctaaaaga
     1261 gaaggatggt tgcttgagtc tcgtaactcg tgaaagtttg gctttggagg tttttcagca
     1321 tacctcttct tacgacaacg tgattgctgg ttactttaat cgagaggttg ttaaaagcga
     1381 agatatattt ccagcaaatt taatctcaag ttatagaaaa gttcactctc taagatatgg
     1441 ggaaaacccg caccaaaagg cggcttacta cagaggtgag ggcttccctg gtccgagtgt
     1501 gataacttcg gagattttgt cgggcaagga aatctccttc aataacttag ttgatctgga
     1561 cgcagctttg ataatgatag aggatttcac tagacccttt gcagtgattc tcaaacatac
     1621 caatccctgc ggtgcggctt gtgcagacaa cttggcagat gcctatagtg acgctctggc
     1681 gtgcgacccg atttcagctt tcggctgcat tatcggttta aataaaatag ttgatatgga
     1741 aacagctaaa aagatcgatg agactttttt tgtggaatgt gttgttgctc cgggatataa
     1801 agaggaagct ctagagttgc ttaagaaaaa gaaaaataga cgctttctgg cttgtcctca
     1861 attattgaaa gatagaaagg gagagccaaa tcaaatcaaa ataataaagg gtggggcttt
     1921 ggttcagact ccggataatc tggaaacaac cgcaagggat ctaaaggtag ttacagaagt
     1981 ttcgccgacc cctgaacaga taaaatccct tcttttcggc tggaaggtgg ttaagcatgt
     2041 gaaatcgaat gccataattt tagctcaagg ggaaaggaca gtgggaatcg gtgcgggtca
     2101 gatgagcagg gttgattcct ccataatcgc tgtgaggaaa gcaggggaaa gagcaaaggg
     2161 ttcggtttta gcttcagatg cattcttccc tatgagggat ggagtagatg cggctgcaga
     2221 ggcaggtgtt gttgccataa ttcagcccgg tgggtcaaaa ggggatgaag aagtaattaa
     2281 agcggcaaat gagcataaga tagcgatggt ctttaccggg gtaagacatt ttaagcattg
     2341 agaattcttg tcgtgacccc ttggaaggct gtccaaaaaa tgtcattctg agtcccctat
     2401 ggggacgaag aatctcaaat ttgcagcaca ttgagtttat tagattcttc acttcgttca
     2461 gaatgacaag ctcggaagtc ctttgaccca acttatagtt ataaggactg aaaagtgtga
     2521 aataaatcgc ccaataaatt gggcaactac aaaaaacaaa aagagaattc gacttgattt
     2581 tgctttaaat tttgctaaat tgaatttata tgcttaaaga taaaaatatc attctaggta
     2641 tcaccggcgg cattgcggct tataaatctg ctgagatttg tcgccgcttg aaaaaactgg
     2701 aggccaatgt ggttgtggtg atgaccaaag cgggcgcaaa gttcatcacc cctttgacta
     2761 tggagaccct ctcggagaac gaagtcgtaa ccgagctttt tccggagaat aaggttgtca
     2821 aaacgagaca catagaccta gccacttggg ctgacttggt tctaatcgca ccagcaactg
     2881 ccaacataat tggcaagata gcatctggaa ttgcggatga tattctctcc actgtggtta
     2941 tgtctaccct ttcaccggtt ttgctctgtc cttcaatgaa cgagaatatg tatctgaatc
     3001 caatcgttca gaaaaacata cagaaactaa aggatttggg gtacaagttt attgaaccgg
     3061 gaatcgggga tttagcttgt ggaacagtcg gaaaaggaag gttggcggat ttagatatca
     3121 ttctacgtga agtagcggat attctggtta agaagaaaga tttggaggga aagaaaatcc
     3181 taatcactgc cggtccaact caagagccag ttgataaagt cagatttctc tccaatcgct
     3241 ccagtggcaa gatgggctat gctttagccc aggaggcaaa agcaagagga gcaaaaacta
     3301 ttttgattag tggcccaact ggtttgtcta aacctgccgg catgagattt catcaggtca
     3361 aaaccgctgg cgagatgtat tgccaagtca aactctattt gaaggaagcg gatattttga
     3421 ttatggcggc tgcagtatca gatttcgccc cgaaaatcgt tcaatctcaa aaaatgaaaa
     3481 gagaagggaa agagatatct cttgagcttg agccgacaat tgatattctc aaggaagtag
     3541 ggaaaaacaa gaagggcaag cttctggttg gttttgcgct ggaaactgaa aatgaggtgg
     3601 agaacgcaaa agggaaacta aaagagaagg atttggattt aattgttgtc aataatccct
     3661 atgtttccgg ggcaggattt gaagttgata ccaatgtggt aaccttgata gacaaaaagg
     3721 gaaaaataga aaagttgcct ttaatgccca agaaaattgt agctgaaaag atattggata
     3781 aagtcgtaag cttattgaaa aagccaagta aaaagaaaaa tgaacgaaaa taagaaaaag
     3841 tttctagatt tggttgacct cacagaaaaa tatgtgaaac aacagataga tttgggcttt
     3901 gatgagcttc ataaagagag aaagccggaa acaaaatcag aagtagaacc agaaacgcag
     3961 cagataaccc tcaaagaatt tcatgaaaag ataaagaact gcaccgaatg tcatcttcac
     4021 aaggccagaa ccaatttcgt cttcggaaca ggtaaagatg atgcagatat tatgttcatc
     4081 ggagaagctc cgggcagaga agaggacttg aaaggagaac cgttcgtggg agaggcgggg
     4141 aagctgttga ataagatttt agcagcggtg aatttcaagc gggaagaggt atatattgga
     4201 aatgttctca aatgccgtcc tccggaaaat agagagcccc ttccagagga gatagaaact
     4261 tgcgagccgt atcttctaaa acagatagcc ataatcaagc cgaagataat ctgtgctctg
     4321 ggacgaatct ccgctcaagc attacttaaa accaaacttc ctttgaataa actgaggggc
     4381 agatttcacg attatcacgg aaggaagctt ttggtaacct atcatccagc tgcgcttttg
     4441 aggtaccccc aatacaaaaa agatacctgg gaagacatac aattgttgag aaaagaatac
     4501 gacaaaatga tttctccgca ggacctatag gagaggtaag atatgaatca aaaagaaaca
     4561 tctggcgaga gagtgccgcc gcaggcaata gaagttgagc aggcggtctt gggaacaata
     4621 cttctggacc gggaggcaat tggcagagcc atagagatta tcgacgaaga gtgcttttat
     4681 cgtgaggctc acagcaaaat ctattcagcc atcgtttccc tgtatgacag aaacgaggca
     4741 gtggatatcg ttacagcaac agaagaattg actaaaagag gtcaattgga ggaagtgggc
     4801 gggaggattt atctcatcaa cctgacggaa agtgtagcta ctgccgccaa catcgaatac
     4861 catgccagga taatcttgga gaaagctacc cttcgcaggt tgattagtgc cagcagtaag
     4921 atagtttcac aatgttacga cactacccaa gaagtggatg aactattgga taaagcagag
     4981 caggaggtct tcagcatctc cgaaggaagg ataaaggaag ggtttgtccc tataggtgag
     5041 atacttcctc gcacctttga agctattgat gaatacgcca aaaggggtgg tattgtaacc
     5101 ggaatagcta ccggcttcac cgagttggat gtcttaactg gcgggttgca gaactcggac
     5161 ctggtagtaa ttgctggaag gccctccatg gggaaaacct ctctggcact atccatagct
     5221 gaacatgtgg cagtggatga aaaggtgccg gttgccatat tcagtttgga gatgaccaaa
     5281 agtcaattgg ctcaaaggat gttatgtagt cgagcccgtt tcagcactca taaaatgaga
     5341 acaggtcgaa tcagcgacca cgagtatggc aatttggcta tcgctgtagg acctctttct
     5401 gaagctaaaa tatttctgga tgatactccc agcttgggaa ttctgcaaat gagagcaaaa
     5461 gccagaagat taaaggctaa ggaaaacatc ggtctggtga tagtggacta tctccaattg
     5521 atgcaaggtc ccagggcgat agaatcaagg caacaggaga tatcgctaat ctctcgttcc
     5581 ctcaaaggat tggctaaaga gctggatgta cctgtagttg cgctttctca gctttcacgc
     5641 gcagtagaga ttagaggagg agagagacgt ccgcaattag ccgacttaag ggaatccgga
     5701 gcaattgagc aggatgcaga cctggtgatg ttcgtctatc ggccggagat gtacaatata
     5761 aaccaattca gagatggcag gtccaccgaa gggatagcag agataatagt tagcaaacat
     5821 cgaaatggtc ccacaggtga aatcttgctc actttcataa aggattatgc gcgctttgaa
     5881 aatcctgagt tagtgcatac tgaaactcca tcttaatccg aggagaaaaa gatcagattc
     5941 atttcccaga gtgagaatcc atttcgcctg attggcaaaa aagctctcac tttttttgaa
     6001 gatgccggca gaatggctat tctaataaag aacctcttga ttaaccttcc gtcggctctc
     6061 acccatgtga gtttagtgac tgagcagatg ttgatcatgg gtgtgaattc gcttcccctg
     6121 gtagtgttca cctcaatctt caccggtgga gtgtctgcag tccaggctgc ttatcaattc
     6181 tccgactata ttcccatgcg atatttggga actgcagtgg gcaaggcagt agtcatagag
     6241 ttaggaccag tcttaaccgc cttggtagtg gtgggtagag tgtgtgcctc tataacagct
     6301 gagttgggaa cgatgaaagt gactgagcag atcgatgcta tggaaacttt ggctttggac
     6361 ccggtgaagt atctggtggt gccacgtttt gtagcgggct ttgtcatgct gccggtcttg
     6421 gtcctatttt cggatttcgt tgctattttg ggtggtctgg cggtctcggt ctttttcttg
     6481 ggaatctcct cttatacttt cctgaatggc ttaaagatgt tcttccattt aagcgatgtt
     6541 ttcggcggac tgcttaaggc attcgttttc ggcagcatca ttgccatcgt aggttgttat
     6601 cagggattta gaactgaagg aggagctgaa ggcgttggcc gctcaactac ccgtgccgtg
     6661 gttctggcat cagttctggt tttgatttca gactacatta tcgcttcgct attgtttgga
     6721 taagaaaaga aaagctatga ttgagataaa cgatttatac aagggctttc ataacaagcc
     6781 ggtattagaa ggagttaatt tagctatcca gaaaggggag acggttgtca ttattggaag
     6841 gagtggttgc ggaaagagtg ttcttctgaa gcatatcatt ggtctcttga agccggataa
     6901 agggcagctt ctcatagacg gggaggacat caccgcttat ccaaaagata aactgaatca
     6961 actccgacag aggtttggca tgctcttcca aggagcagct ctttttgact ctatgactgt
     7021 ggcggagaat gtgggattgg gtttaagcga gcatactgaa atgacagagg ggcagataag
     7081 agagatagct gcagagaagc tgaaaatggt cggtttagtg ggagtggagg acttgaaacc
     7141 agctgagctt tccggaggaa tgaaaaaaag ggtgggattg gctcgcgcta ttgctatgaa
     7201 tccggaattc atcttatatg acgaacctac aaccgggctg gaccccataa tggcggatgc
     7261 gataaacgac ttggtaatca atttacgtaa caccctcaag atcacctcaa ttgcagttac
     7321 gcatgatatg gcttcagctt ataaaatcgc tgacaggatt gctatgctct ataaaggcaa
     7381 gataatattc gctggcacac cagaagagac caaaaactct gacaatccgg tagttcaaca
     7441 attcattcag ggtcgctctc agggaccgat acccactctg gagtaaaatg gctatttcgg
     7501 acaaaataaa aaccatctac atttgtcaag tttgtggcta caagtcccca agatggttag
     7561 ggaaatgccc tgagtgtggg acctgggata gtttgatcga ggagaaaatc actccgacta
     7621 agagatggga agagcccggg ggacaacttg agatgccgat tcctgtaact aagatcacca
     7681 aagacaaaga agagagaatc atcacaggtg caagagaatt tgatagagtg atgggtgggg
     7741 ggattgtgaa tggctcagtt accctggtgg gtggagaccc ggggatagga aaatccacct
     7801 tacttctgca aatcagcgaa agcgttgctc tcaaaaacaa aaaagtcctt tatgtaagtg
     7861 gtgaagaatc tgccaagcaa gttaaaatca gagcagagag gttgggggta ttatccgaca
     7921 atttggaagt tttggcagag aataatctgc attacatctt agctgtgctg agccagagga
     7981 aatttgatgt agtgataatt gactcaattc aaacctccta tactcaggaa ctggagagcg
     8041 caccgggtag tgtcagccaa atcagagaat gcgcttaccg gtttatcaat tacgctaaaa
     8101 ccagtgggag ttcatttttc ttgatcggtc atgtgactaa agaaggagcg atcgctggac
     8161 caaaagtgtt agagcatatg gttgatacgg ttctgtattt tgaaggcgag aaaagtcata
     8221 cctatcgcct tttaaggtca gttaagaatc gatttggctc aaccaatgag ctgggcattt
     8281 ttcagatgat ggcaaaagga ctggtcgaag ttgagaatcc atccagagct ttcctgtccg
     8341 aaagaagaaa ggattctccc ggttcggtag tgatatgcag tatagagggg acaagacctc
     8401 tgcttttgga attgcaggca ttagttactc cctccacctt cgggatgccc cagagggttt
     8461 gttccggggt tgattataaa agggtatctt tacttttggc ggtgttagaa aagatgggcg
     8521 gaatcagtct ttccaagtat gatgttttcg tcaatttggc cggtggagtg agaatcgaag
     8581 aaccggctct ggacttgggg ataataactg cgatcgcttc cagctaccgc gatatgccca
     8641 tagacccgga ggcagtggtg ataggagaag tgggattaag cggagaaata aggacggtag
     8701 atcagataga aaggaggatt aaggaagcgg aaaaactcgg tttcaaaaga tgtattttat
     8761 ctcaagggaa tttgaaggaa atagaaaata aattctccat tcaggtaatc ggaacaagag
     8821 agataaacga aacgttcaga aaactcttta tacagaaagc gaggagaagt gagactgaag
     8881 ataagtaagg cgcttcgcgg cggagaatat ttggtgagtt ttaaaactct gggtttcagc
     8941 ccggaggaag aggaaaaggc aggaaggttt ggctcaccgg agatcgattt cagcttggac
     9001 gggctgggca cgagaaaatt ggagggttta gacatctcgg tgaaatgtga aagtccagag
     9061 aaagcagagg aattcatcct tcaaaccaaa gataagataa aagaaagatt aactcaactt
     9121 aaagatatgg tggatacttt cagtggggag gaggagttaa gtttataaaa atggcaaaaa
     9181 aacaattgcc ccctcaagcc cttctgatac ctttgcctgt ggttatggtc agctgtcagt
     9241 ctaagggagg accgaacatc ataaccctgg cttgggctgg agtggtttgc tctgagccac
     9301 ctatggtggg gataagcata agacccagca gatattcttt tggtatcata agagattcaa
     9361 aagagtttgt attaaatgta gtaaatgagg atttaatcag acaggcagat ttctgtggca
     9421 gccattctgg tagagatgtg gataaattta aagagctgaa cctaacccca atcaaagggg
     9481 aaaaggtcaa atcccctcta atcaaggagt ctcccattaa cttggaatgt caggttaaag
     9541 atataatcga attgggcact catcaccttt tcctgggtga gatagtggcg acacatatag
     9601 atgagaggta cttggattcg aatggtaatc cggatattgc taaaatcagg ccggcagtct
     9661 attgcactaa agcgcatcag tactggggtg ggctatcaaa agtattggga acttacggat
     9721 ataccgccaa aaagagatag aaaatgcaaa aggggttaat gctttaatcc gtttgggtta
     9781 aacgttaccc cctttaattt taactataca aatgcatagc ttcgatttca aattagaggc
     9841 aaaagatagg gcatccaaag cccgggctgg aatcataaat actcctcatg gtcagattaa
     9901 tactccagtg tttatgccgg ttggaactca agcttcggtc aaaactttat gctctgagga
     9961 attggacaac ttaggtgcag agataatctt gggtaatagc tttcatctct atttacgccc
    10021 aggaattgaa gtcatttcac aggcaaaggg attgcaccaa tttatgagct ggcacaaaag
    10081 tatcctgact gacagcggag ggtatcaagt ctttagtcta aattcactta ataaagtctc
    10141 agaagatggg gtcgcattta aatcttatct ggacggctca atccataact tcactccgga
    10201 aaaaataatt gaaatacaga tggctttggg gtcagatata attatgcctc tggatgtgtg
    10261 cgccccttat ccttgctctg gggaatatgc caaacagtca agcgacctaa ccctgaaatg
    10321 ggctaagagg tcgaaagata gactggttag tttcagagag gataaaacct taccggctct
    10381 gttcggcata atccagggaa gcacttattc tgacttgagg ttggagtcag tcaagaaaac
    10441 agtggaattg gattttgatg gttatgccat cgggggatta tccgtgggtg agccaaagaa
    10501 cagtatgtgg gagatagtgg aagtggtatc aaatcacctt ccttcggata aaccgcatta
    10561 cttgatgggg gttgggacgc ctttagacct ggttgaagca atctcacgag ggatggatat
    10621 gtttgactgc gtgcttccca cgcgcaatgc cagaaatgga acagttttta caaagaaagg
    10681 gaaaatcatt ttgaaaaatg ccaaatatca gaaggatttt tctcccttag acgagacctg
    10741 tcaatgtcta acctgtaaaa attatagtcg tgcttatttg aggcacttgt ttatgagtga
    10801 agagataagt gggttacgtt tagccacaat tcattcgctt tatttttaca taaatttgat
    10861 gaaagaaatg agagaggcaa tagttttggg taattttgaa gaatggagaa agggcttttt
    10921 tgcgagttat tcagaaaatg agagtgtggt gaaaaatggg ttggagtgag attttctaag
    10981 gttggcttgg gaattgtcac cgggatcccg tttggcggga gatttaccga acccaatgat
    11041 gagcgagaat tccccgacac gggtgttgac gacaattccc gctcaaacga ggatcctccc
    11101 tggccattgg gtcgagcggg gtgtaaaccg ttctggttca caccggggag aaacgggcag
    11161 gctgacccga caggggagtt tgtagcggtc gcatttatgc gacagttcgt ttgataaatc
    11221 cctggccggg caggtaaacc gcagaagcct tggaccaccg caaaggttat cgttatgtat
    11281 ggaaacctaa aggtttccgc tacaagggtt ttaatgtagg gatagaacac tgttctgtcc
    11341 ctactgcatt aacctctccc tagccccctc ctttttgagg aaagggaaca taattaattt
    11401 ctatttttgt atttatttgg acttgatgaa acagatgaaa aaggttatat tagagggcag
    11461 gttcgtcgat tggaaaaaga gatttgaaat ccaatacgga gaggaaaaca atctttaatc
    11521 ggaggcatta tgttcgaaac cgcgtatgct atgagtagtg gtggtggtgg aacaccgggc
    11581 aagggattgg aattcaccat cctttggctt gttctgattt ttgggatctt ctattttcta
    11641 atcattcgtc cccaacagaa aagacaaaag gagcagcaaa agatgataag ccagctccaa
    11701 aaaggggata gggtggtaac taatagtggt atgtttggca caattgtagg gataaacgaa
    11761 aaggagaacg ttgtagtttt gaaaataggt gaagatgtaa aagtggagtt cctgagaagc
    11821 tcaattgctg gaaaagttgc gaaaggaagc tgattatggc aaggttgcca ataagactat
    11881 atggtgaccc gactttgaga aagagagcgg aaaagataga ggagattagc caagagataa
    11941 aaaacttagc caacgatatg ctggagacct gtaaagaggt caaggcaatt ggcctggctg
    12001 gaaatcaggt tggcgtggcg aagatgattt tcgtggtgga caggactagc gtagatttga
    12061 acgagaagcc cttggtggtg ataaatccgg aaatcgtgga ggtagagggt gaagacttgg
    12121 gcgaagaagg atgtatgtct gtgccgggtg cttttgaaca ggtgaaaaga ccacacaagg
    12181 tcaaaatcaa aggattggat ctggaaggaa aagaagtaac aatcgaggga gaagggttat
    12241 tagccagggt cttgatgcac gagatagacc atctaaacgg acttcttttc attgaccact
    12301 tgagctcgat cagaaggcaa cttttgagta aaagattaag ggaaatatcc agagagggtt
    12361 catcggaata gttatatgca catagttttt atgggaacac cggagttcgc cattcccagc
    12421 ttagaaaagc ttctgcacag cagacacaag gtaatagcag tcgtgaccgg ctcagataag
    12481 cccaaaggaa gaggccaatt ggtctgcgca actccggtaa aagagtacgt ctcgaaatac
    12541 aagattccag cactatgtcc aactgatttg aaagcagatg attttttcaa aagattatca
    12601 tctctcgagc ctgagctggg tgtggtggtg gcttttcgca tactccccca gagcattttc
    12661 gatcttcctt cgaaagggac aatcaatctt cacgcctcac ttcttcccaa atacaggggc
    12721 gcggcaccta taaactgggc tttgatcaag ggtgaaaaca aaaccggctt aactactttt
    12781 ttccttcaag agaaagttga taccggcaac atcattctgc agaaggaagt agatattcta
    12841 tccaatgaaa ccgcaggtga gctttcccat agattagcag agctgggagc tagtcttctt
    12901 ttggagacga tagatttaat cgaaaatgga aaattcgaac tcaaaagaca ggacgactct
    12961 caagtgagcc ttgctccaaa gataaccaag gagatgtgca agatagattg gtcaaagagt
    13021 gccctccaga taaaaaactt gataagagga ttgtcccctg ttcccgcagc atacagtata
    13081 ttcaagggaa aaatcttaaa agtatatcga gctgaaatct taaatgaaaa gtctcctcag
    13141 gcaaaaagtg gagaaataat aatatctaat cgtatgaagt ctcttattgt gaaagccaaa
    13201 gataaagctt tggagctaat ggaaatccag ttggaaggaa aaaagaagat gaagggggag
    13261 gagtttatta aggggtgtcg ttttcaagct tgtgagaaat tagattaacc caaaaggagg
    13321 agtttggggt tgggaaaatc actttttctg agattatcca tcttaagtct gatggtcttg
    13381 ttcatactga ccggctcttt atggtttttc atttcccccc ggatttacga gataaatcct
    13441 gccttagccc taatagttaa gcttatcctg gttttgtttt tgttagccat aactggtgga
    13501 ttgtttttaa tcgtcctctc ctcgatcacg gaaaaggatt ttctctttcc acataacgag
    13561 aagcagataa ccttgaagat tctttttccc ataaatcttt ttctaggaca gctttttggc
    13621 atatctaagg atagaattag agaatctttc attaaagtga acaactcctt ggttaaagcc
    13681 actcgcaaga ggataagaaa cgatagagtc ctgatactat tgtcccagtg cttacagaat
    13741 tacgattgtc ctaatcgggt tacagttgac tggcgaaact gcaagagatg cgtgaaatgc
    13801 gttttaagcc agataattga attaggagaa aagaataacg cctcggtggc gattgccact
    13861 ggtggcaccc tggctcgtaa ggtgatagtt gacaccaatc ccaccgccat tgtggcggtg
    13921 gcttgccaaa gagatttaac cagtgggatt caggatgttt atcctatacc ggtttatgga
    13981 gttttaaatc aaagacctta tgggccttgt gttaatactc aagttgactt cgaagaacta
    14041 aaaaaagctt tgcagttcat atcagatggg aggagttagt tgcagaaaga tatcgtcata
    14101 aacgtttcag aatatgaaac ccgcctggcc attttagagg atttgaaatt agtggagctt
    14161 ttgatggaga ggcctgaggc ggagcgcatg gtgggggata tttacaaggg agcggtcaaa
    14221 gacgtcttac ccggaatgca agccgcattc gtagatatag gcataggtaa gaatgctttc
    14281 cttcacgttt cagatgttgg tctagtagaa gaaagtggaa caagtcgaaa ggaaagaaaa
    14341 atagaagatg taatcaagca ggga
//
LOCUS       DASWLN010000141         1814 bp    DNA     linear   ENV 20-FEB-2024
DEFINITION  MAG TPA_asm: Candidatus Zixiibacteriota bacterium isolate
            SMAG_U2949 k141_108825, whole genome shotgun sequence.
ACCESSION   DASWLN010000141 DASWLN010000000
VERSION     DASWLN010000141.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37960594
            Sequence Read Archive: SRR10376091
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Candidatus Zixiibacteriota bacterium (soil metagenome)
  ORGANISM  Candidatus Zixiibacteriota bacterium
            Bacteria.
REFERENCE   1  (bases 1 to 1814)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 1814)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (26-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/12/2024 08:28:26
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 1,440
            CDSs (total)                      :: 1,416
            Genes (coding)                    :: 1,412
            CDSs (with protein)               :: 1,412
            Genes (RNA)                       :: 24
            rRNAs                             :: 1 (16S)
            partial rRNAs                     :: 1 (16S)
            tRNAs                             :: 21
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 4
            CDSs (without protein)            :: 4
            Pseudo Genes (ambiguous residues) :: 0 of 4
            Pseudo Genes (frameshifted)       :: 0 of 4
            Pseudo Genes (incomplete)         :: 3 of 4
            Pseudo Genes (internal stop)      :: 1 of 4
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..1814
                     /organism="Candidatus Zixiibacteriota bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_108825"
                     /isolate="SMAG_U2949"
                     /isolation_source="soil"
                     /db_xref="taxon:2053527"
                     /environmental_sample
                     /country="USA: Oregon"
                     /lat_lon="42.99 N 123.63 W"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            complement(<1..344)
                     /locus_tag="VGB16_04790"
     CDS             complement(<1..344)
                     /locus_tag="VGB16_04790"
                     /inference="COORDINATES: protein motif:HMM:NF024724.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="HD domain-containing protein"
                     /protein_id="HEX9917036.1"
                     /translation="MNLAEFIIKVESFNANIDIPLIRKAYEFSDRIHRGQLRQSGVPY
                     VEHCLEVAFILAEQHLDSTTIAAGLIHDVVEDTEATLAQLKTEFGEEIAELVDGVTKI
                     SELEFRSQEEEQA"
     gene            complement(341..1414)
                     /locus_tag="VGB16_04795"
     CDS             complement(341..1414)
                     /locus_tag="VGB16_04795"
                     /EC_number="2.4.-.-"
                     /inference="COORDINATES: protein motif:HMM:NF013257.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016757 - glycosyltransferase activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="glycosyltransferase family 9 protein"
                     /protein_id="HEX9917037.1"
                     /translation="MKRITLKGDEKILIVRTDGMGDVILSIPTLELIKSNYPDCHLAM
                     LVSPYTVPLLENNPYLDEVLTDDKNDKHKGIRGFLVLAGLLRDRKFDIAVALHPSWRV
                     AILLILARIKYRVGTGYRFYSFFFNLKLFQHRKDIQKHELEYNLDMLGPLSINPEKIL
                     PKVYLSTQEKEKGNVLLEKLGIKSDNLLVVVHAGSRNSSLNFPPEKFAQVADELVEKE
                     GVNVVLTGSIGESSLVKSAKNRMKQNSINLVGKLGLRELAILLKRADLMIANSTGPMH
                     LAVAIGTPVVAIFCPIFVASPKRWGPYGENNQVVLPPVPICQKCKPKRCRYYNCMDKV
                     DTEEVYLKAKSLLNWKKKLEEKG"
     gene            complement(1411..>1814)
                     /locus_tag="VGB16_04800"
     CDS             complement(1411..>1814)
                     /locus_tag="VGB16_04800"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=3
                     /transl_table=11
                     /product="glycosyltransferase family 2 protein"
                     /protein_id="HEX9917038.1"
                     /translation="WIKHSGWYPDYVLRLFKRDKGSFTQNRVHERVIVDGTGGYLKNE
                     LLHYTDPNLHHYLIKLNQYTTDAALEMHQQGKEASLSDMVFRPMGIFLKMYLLRVGFL
                     DGMEGLILAFCSAFHVFFKYAKLWHLNKSKP"
BASE COUNT          501 a          440 c          316 g          557 t
ORIGIN      
        1 gcttgttctt cttcctggct tctgaattcc agctcgctta tcttggttac tccatctact
       61 aactccgcaa tctcctcccc aaactccgtt ttcagttgag ctaaagttgc ttcagtatct
      121 tcgactacgt catgaatcaa accagccgca atggtagttg aatctagatg ttgctctgcc
      181 aagatgaacg ccacttccag acaatgctcc acataaggga caccggactg acgaagctgc
      241 ccccggtgaa tcctgtctga gaattcgtat gctttacgaa tcaaggggat atcgatattg
      301 gcgttgaatg attcaacttt tatgatgaat tcagctaagt tcatcctttc tcctctaatt
      361 tctttttcca gttcaacaat gactttgctt taagatagac ctcctcagta tccactttgt
      421 ccatacagtt ataatatcgg caccttttgg gcttgcattt ctgacagata gggactggtg
      481 gcagaacaac ctggttattt tctccataag gtccccaacg tttagggctg gcaacaaata
      541 tcggacagaa aatcgccacc accggagtac caattgcaac tgccagatgc atcgggccgg
      601 tgctgttggc gatcatcaaa tccgcccttt tcaaaagtat agctaactcc cttaagccaa
      661 gtttaccaac caagttaatt gaattttgtt tcatcctgtt ttttgcggat ttgaccaaac
      721 tggattcgcc tatgctcccg gttaagacta cattcacccc ctccttctca actaactcat
      781 ctgcaacttg agcaaacttc tccggaggaa aatttaagga tgaattcctg cttcccgcat
      841 gaaccaccac caacaggttg tctgatttaa tgcctaactt ttccaaaaga acgttgccct
      901 tctctttttc ctgagttgag agatagactt tgggcaggat tttttctggg tttatgctca
      961 aaggtccaag catatccagg ttatattcca attcatgttt ttgaatatct tttcggtgtt
     1021 gaaacagttt caaattgaaa aagaaactgt aaaaacgata tccggtgcca actcgatatt
     1081 ttattcttgc caaaatcagt aagatagcca ctctccagct cggatgaagc gcaactgcaa
     1141 tatcgaattt tctatcacgc aaaagcccgg ctagtaccaa aaaacccctt atccctttat
     1201 gcttatcatt tttatcatct gttagcacct catccaaata aggattattt tccaaaagtg
     1261 gtacagtgta aggggagacc agcatagcca gatgacaatc aggataattg ctttttatca
     1321 attccaaagt gggaatagag aggataacat cccccatccc atctgtgcgg acaatcaata
     1381 tcttctcgtc gccttttaaa gttattctct tcatggctta cttttgttta gatgccacaa
     1441 cttggcatat ttgaagaaaa cgtgaaaagc tgaacaaaaa gctaaaatca aaccttccat
     1501 tccgtccaag aaacccactc ttaggagata catcttaagg aatatcccca ttggccgaaa
     1561 gaccatgtca gataaacttg cttccttccc ttgctggtgc atttccaaag ccgcatcagt
     1621 ggtatattgg tttaatttta ttagatagtg atgtaaattc gggtcggtat aatgtaaaag
     1681 ctcatttttc agatatcccc ctgtcccatc tactattact ctctcatgca ctctattctg
     1741 agtgaacgac cctttatccc ttttaaagag tctcagaaca taatccggat accagccact
     1801 atgctttatc catt
//
LOCUS       DASWLN010000142         1937 bp    DNA     linear   ENV 20-FEB-2024
DEFINITION  MAG TPA_asm: Candidatus Zixiibacteriota bacterium isolate
            SMAG_U2949 k141_820424, whole genome shotgun sequence.
ACCESSION   DASWLN010000142 DASWLN010000000
VERSION     DASWLN010000142.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37960594
            Sequence Read Archive: SRR10376091
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Candidatus Zixiibacteriota bacterium (soil metagenome)
  ORGANISM  Candidatus Zixiibacteriota bacterium
            Bacteria.
REFERENCE   1  (bases 1 to 1937)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 1937)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (26-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/12/2024 08:28:26
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 1,440
            CDSs (total)                      :: 1,416
            Genes (coding)                    :: 1,412
            CDSs (with protein)               :: 1,412
            Genes (RNA)                       :: 24
            rRNAs                             :: 1 (16S)
            partial rRNAs                     :: 1 (16S)
            tRNAs                             :: 21
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 4
            CDSs (without protein)            :: 4
            Pseudo Genes (ambiguous residues) :: 0 of 4
            Pseudo Genes (frameshifted)       :: 0 of 4
            Pseudo Genes (incomplete)         :: 3 of 4
            Pseudo Genes (internal stop)      :: 1 of 4
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..1937
                     /organism="Candidatus Zixiibacteriota bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_820424"
                     /isolate="SMAG_U2949"
                     /isolation_source="soil"
                     /db_xref="taxon:2053527"
                     /environmental_sample
                     /country="USA: Oregon"
                     /lat_lon="42.99 N 123.63 W"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            complement(175..1539)
                     /locus_tag="VGB16_04805"
     CDS             complement(175..1539)
                     /locus_tag="VGB16_04805"
                     /inference="COORDINATES: protein motif:HMM:NF016232.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005524 - ATP binding [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="ATP-binding protein"
                     /protein_id="HEX9917039.1"
                     /translation="MVQIKAYSQKELAFLFFGPYDLRLTVKRVRVEGLRQFNAVKDIV
                     DENKGFYGFAELRGAYIKAPDGWRNALTKVVLLKKDAKKEPDEEVNYGGFILIKKTIP
                     LDVFLKTAEKLFSESILMLDKFSCQLAPPSISTWDYPSNDFYDWAGKLFEISGGKLSI
                     PERPPLVDYSNPPFQDAYHAISKWLDLFPFHRTSDARLGSVLVFLPEYRARIKSIRYA
                     DKKLSLKAEFNIPKLTDVRCKAVIRTSSGQEIQSSLDFKTDETEFPLESPPDETYVYL
                     VTSDNRILDTYEETPYRHSGRVRLIANEVGLADIEVLILAGENEMVEFKERVEEDKTK
                     KDLIRTAVAFANTKGGKIFIGISDNAEIIGVNREIEKDGESKLKEKFDSILRDNVRKP
                     LKYSMEVRKVSDKSLLIIHVQEGEFKPYATRDNEIFIRVGGTDRRPDPDTELPALFPK
                     YKSK"
BASE COUNT          550 a          429 c          390 g          568 t
ORIGIN      
        1 aacaactgga gaaaccacta gagtatcata cactttatat ttatatataa cagcctcttt
       61 tgaaatctta atatttcatt tttatccctt gttactttcc ttatcctaaa ctgtttcaat
      121 gaatgtagga agatcccaaa gtagaatgat ttgttaatag tgtagacctg acacctattt
      181 acttttgtat tttgggaaga gggctggaag ttcagtatcc gggtctggcc ttctgtcagt
      241 ccctccaacc ctgatgaaaa tttcgttatc ccttgtggca taaggcttaa actctccctc
      301 ctgaacatgg ataattaata gagacttgtc acttacctta cgaacctcca tagaatactt
      361 gagaggcttc ctcacgttat cccttagtat agagtcaaat ttctccttaa gcttggattc
      421 cccatctttt tctatttcac ggttgacacc gatgatttct gcgttatcgc taattccaat
      481 aaagatttta cccccttttg tgtttgcgaa tgcgacagct gtccttatga ggtccttctt
      541 cgtcttatcc tcttcaaccc tttccttgaa ctcaaccatt tcattctccc ccgcaagaat
      601 cagaacttct atatcagcta agccaacttc atttgctata agcctaactc tcccgctatg
      661 cctataagga gtttcttcat aagtatcaag aatcctgttg tccgaagtca caaggtaaac
      721 gtatgtctca tcaggagggg actctaatgg aaactctgtt tcatctgttt taaagtccag
      781 agaggactga atctcttggc cagaggatgt tcttataact gctttgcaac ggacatcagt
      841 caatttagga atattaaact cggcttttaa agagagcttc ttgtcagcat atctaatgct
      901 ttttatcctt gccctatatt ccggcaggaa caccagcacg gagccgaggc gcgcatcaga
      961 agttcggtgg aaagggaaga gatccaacca ttttgaaatg gcgtgatagg catcttggaa
     1021 aggagggttt gaatagtcca ccagtggtgg gcgttccggt atgcttagct tccccccgct
     1081 tatctcaaaa agtttgcctg cccagtcata gaaatcgttg ctgggataat cccaagtcga
     1141 tattgaaggc ggggctaact ggcaggagaa tttatcaagc attaggatac tttcggaaaa
     1201 gagcttttca gcagtcttta gaaaaacatc aaggggaatg gttttcttaa tcagaatgaa
     1261 ccctccgtaa ttaacttcct cgtcgggctc tttcttcgca tcctttttaa gcaagacaac
     1321 tttggtgagg gcgttcctcc acccgtcagg agcctttatg taagcaccac ggagctcagc
     1381 gaagccgtaa aatcccttat tctcgtctac tatatctttg acagcgttga attgtcttaa
     1441 gccttccacc ctcaccctct ttaccgtaag gcgtaagtcg taaggcccaa aaaacaaaaa
     1501 agccaattct ttttgagaat aggctttaat ttgaaccaat catttggata ccccaccgga
     1561 ttttgcccca ctatatcaga aacgcaacgg gattacaata attaagcatt ttttgtgcca
     1621 cgagaaaagc agagcgcata gagcatagcg cccgcctacg ccaaaagctt cggcaaggta
     1681 aacatagcgc aaacctatga aataacacat aaaatttagt tcattctcgt ttcaaggaaa
     1741 caattctctc taatcaattg aattggggtg gtcagaaatt ggccaaaaat gtcctaaacc
     1801 ttgtgagttt gtgaaacgga caaggaacat cttagagtgc ggagtgtgga ttgtggaata
     1861 tagaaagcaa aataaggaac taggaaatta aaggattagg gatctgggga gacgcgggga
     1921 cacggtgaca tggggac
//
LOCUS       DASWLN010000143        13931 bp    DNA     linear   ENV 20-FEB-2024
DEFINITION  MAG TPA_asm: Candidatus Zixiibacteriota bacterium isolate
            SMAG_U2949 k141_369853, whole genome shotgun sequence.
ACCESSION   DASWLN010000143 DASWLN010000000
VERSION     DASWLN010000143.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37960594
            Sequence Read Archive: SRR10376091
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Candidatus Zixiibacteriota bacterium (soil metagenome)
  ORGANISM  Candidatus Zixiibacteriota bacterium
            Bacteria.
REFERENCE   1  (bases 1 to 13931)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 13931)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (26-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/12/2024 08:28:26
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 1,440
            CDSs (total)                      :: 1,416
            Genes (coding)                    :: 1,412
            CDSs (with protein)               :: 1,412
            Genes (RNA)                       :: 24
            rRNAs                             :: 1 (16S)
            partial rRNAs                     :: 1 (16S)
            tRNAs                             :: 21
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 4
            CDSs (without protein)            :: 4
            Pseudo Genes (ambiguous residues) :: 0 of 4
            Pseudo Genes (frameshifted)       :: 0 of 4
            Pseudo Genes (incomplete)         :: 3 of 4
            Pseudo Genes (internal stop)      :: 1 of 4
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..13931
                     /organism="Candidatus Zixiibacteriota bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_369853"
                     /isolate="SMAG_U2949"
                     /isolation_source="soil"
                     /db_xref="taxon:2053527"
                     /environmental_sample
                     /country="USA: Oregon"
                     /lat_lon="42.99 N 123.63 W"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            complement(8..1213)
                     /gene="tuf"
                     /locus_tag="VGB16_04810"
     CDS             complement(8..1213)
                     /gene="tuf"
                     /locus_tag="VGB16_04810"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_012096692.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003746 - translation elongation factor
                     activity [Evidence IEA];
                     GO_process: GO:0006414 - translational elongation
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="elongation factor Tu"
                     /protein_id="HEX9917040.1"
                     /translation="MSKKRFERTKPHLNIGTIGHVDHGKTTLTAAITMVLHRKNMADV
                     RTFDSIDNAPEERERGITIAVAHVEYETEKRHYAHIDCPGHADYIKNMITGAAQMDGA
                     VLVVSAADGPMPQTREHILLARQVGVPYIIVFLNKVDMVDDPELLDLVELEVRDLLKK
                     YQFPGDEIPMIRGSALEAMTQAAKSEVKDDPSFKCIYELLAAMDNYIPQPKRETDKPF
                     LLPVEDVFSITGRGTVGTGRVERGAIKVGDEVERVGIRETKKTVVTGVEMFRKLLDQA
                     QAGDNVGLLLRGVERGELERGMVIAAPGSITPHTKFKSEVYVLTKEEGGRHTPFFNGY
                     RPQFYFRTTDVTGVVKLPEGVEMVMPGDNANFEVDLITPIAMEKELRFAIREGGRTVG
                     AGVITEVIE"
     gene            complement(1321..1572)
                     /locus_tag="VGB16_04815"
     CDS             complement(1321..1572)
                     /locus_tag="VGB16_04815"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_014856869.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004518 - nuclease activity [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="GIY-YIG nuclease family protein"
                     /protein_id="HEX9917041.1"
                     /translation="MKYYVYVLKSKIVDRYYVGLTKDLERRLTEHNNRKKHWTSRFQP
                     WEIIYCEEFNARAEAMKKERGLKSKDGIVEKLKIIKGIE"
     gene            complement(1584..1656)
                     /locus_tag="VGB16_04820"
     tRNA            complement(1584..1656)
                     /locus_tag="VGB16_04820"
                     /product="tRNA-Thr"
                     /inference="COORDINATES: profile:tRNAscan-SE:2.0.12"
                     /note="Derived by automated computational analysis using
                     gene prediction method: tRNAscan-SE."
                     /anticodon=(pos:complement(1621..1623),aa:Thr,seq:ggt)
     gene            complement(1868..2470)
                     /gene="rdgB"
                     /locus_tag="VGB16_04825"
     CDS             complement(1868..2470)
                     /gene="rdgB"
                     /locus_tag="VGB16_04825"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_015312213.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016462 - pyrophosphatase activity
                     [Evidence IEA];
                     GO_process: GO:0006281 - DNA repair [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="RdgB/HAM1 family non-canonical purine NTP
                     pyrophosphatase"
                     /protein_id="HEX9917042.1"
                     /translation="MTRIVLATRNKNKIKEIKEILGDLKVELLSLEELPGLPEVKETG
                     TSLKENAILKARSVFDFTGLPSLADDSGIEVSALDGAPGVDSARFAGENCTYTDNNRK
                     LLSLLEGVPWKKRRATFVCVAALVLNNKEIFIDEGKVFGYVADKECGDQGFGYDPVFY
                     FPPEKKTFGQMSASQKNRISHRAKAFLRTKKVIRQRLLGS"
     gene            complement(2470..3273)
                     /gene="murI"
                     /locus_tag="VGB16_04830"
     CDS             complement(2470..3273)
                     /gene="murI"
                     /locus_tag="VGB16_04830"
                     /EC_number="5.1.1.3"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_013291081.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008881 - glutamate racemase activity
                     [Evidence IEA];
                     GO_process: GO:0009252 - peptidoglycan biosynthetic
                     process [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="glutamate racemase"
                     /protein_id="HEX9917043.1"
                     /translation="MKIKIGIFDSGIGGLTVAARIFELLPQVDVVYFGDTARYPYGPR
                     SSKVVQKLSYQNTRFLLSKKVDFIVVACNTASAFALDHLKRKFNTPMLGVIEPGARTA
                     VRCTKNKRVGVIGTVGTIASKSYSKAIRKLDKNIRVFTRACPLLVALAEEGYINKKAT
                     TMIVQEYLKPLVRKRIDTLILGCTHYPLLKGVIARVMGNKVKLIDSAEEIAFELKRRL
                     KAKRVTARSNERGAHAFFVSDVPEKFVQVGERFLGRKIRRVQRIEIDRY"
     gene            complement(3270..4757)
                     /locus_tag="VGB16_04835"
     CDS             complement(3270..4757)
                     /locus_tag="VGB16_04835"
                     /EC_number="3.5.1.28"
                     /inference="COORDINATES: protein motif:HMM:NF013670.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008745 - N-acetylmuramoyl-L-alanine
                     amidase activity [Evidence IEA];
                     GO_process: GO:0009253 - peptidoglycan catabolic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="N-acetylmuramoyl-L-alanine amidase"
                     /protein_id="HEX9917044.1"
                     /translation="MNRKLSFLLFFSIISVCIISYALSSVDMEIGGKSLRIDSYEKFN
                     LTYVSITELNEVLKGEVSWNQLTKKGTWQFNGHELILSPFSPYIVVDTTVYNLVREIE
                     FKKGTIYVPLETFKPILGRLVSQEISWGKKERKLSFNPSKMNILDIRASQKTNGILIE
                     IFLSEPLEYHLFLGDSRWVNVNFYEGKLDTAFFSGRTIPKCITEIKAYQFDNAAQLSL
                     KMAKGFDKHLEQMQSNPYCLQVSLEDTAKTAVAIQSSLQKENLEDNRIDVVVIDPGHG
                     GEDLGAVGPSGLVEKDVTLDIAKKLSRYLREEKGLKVMLTRREDVFVALEERSRIANR
                     AGGDIFISIHANSAPRKTATGFETFFLAQSKNDEARAVATLENASLQFEKPKEARKEI
                     SDLDFILLDMVQNEFLKESSELASIIQQELDRKSKRENRGVDQAEFVVLNKTYMPAIL
                     VEVAFISNKEEEKLLKKDSVRDEIAKSLYQSIKRFKQKYETKGKK"
     gene            complement(4754..5224)
                     /gene="smpB"
                     /locus_tag="VGB16_04840"
     CDS             complement(4754..5224)
                     /gene="smpB"
                     /locus_tag="VGB16_04840"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_012301409.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003723 - RNA binding [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="SsrA-binding protein SmpB"
                     /protein_id="HEX9917045.1"
                     /translation="MKEEEENTKVVCTNRKAHYLYEVLESLEAGLVLVGTEVKSLRSG
                     KANLKDSFAIFKNGELFVLNMHVSPYEQGNRYNLNPTRPRKLLLHKKEMRKLFGKVAE
                     RGLTLIPLKLYFKGKVAKIELALAKGKKVYDRKRDIKERDMQREMQRAFREKNR"
     gene            5807..5889
                     /locus_tag="VGB16_04845"
     tRNA            5807..5889
                     /locus_tag="VGB16_04845"
                     /product="tRNA-Leu"
                     /inference="COORDINATES: profile:tRNAscan-SE:2.0.12"
                     /note="Derived by automated computational analysis using
                     gene prediction method: tRNAscan-SE."
                     /anticodon=(pos:5841..5843,aa:Leu,seq:cag)
     gene            5927..6832
                     /locus_tag="VGB16_04850"
     CDS             5927..6832
                     /locus_tag="VGB16_04850"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEX9917046.1"
                     /translation="MNIRGVIVSFLKTIFEFIVRILKFLLAKVLLPIWRFITKNAPQV
                     QALIAVLALGLLAYNITLSIKNRNDSRKFGQQMKGYAEDIRASLAETNKGLKILPSSV
                     ESLDSAVIKVTSTVDRISINQDKFAIATKTLEQNVTLFGDKLAEVIEANKEQLKIWSD
                     SRKALEEELHKVPELEIFSKPISYNQKDSTYSMSVWIRNKGKRAARGFSVFLSIDPKI
                     NARAYNEGVWVVEERVVEHKEWIECNHMEREKPVWPDSNLSINVAALRFKLGDSNMKQ
                     FTVYWQVGHDLATNSGKFKIDIPTN"
     gene            7059..11270
                     /locus_tag="VGB16_04855"
     CDS             7059..11270
                     /locus_tag="VGB16_04855"
                     /inference="COORDINATES: protein motif:HMM:TIGR00241.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008047 - enzyme activator activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="acyl-CoA dehydratase activase"
                     /protein_id="HEX9917047.1"
                     /translation="MKGNIFLGIDVGSIAVKVVLISPNKEILKTFYVRSQGQPLLVVK
                     NAFLEIFKEYDRENILALATTGSGGKTISRILDGYFVNEVIAQVKGTGYLLPKVRTII
                     EIGGEDSKFILLHYDEEAKTSILDDFAMNSICAAGTGSFLDQQANRLKISIEDEFGQL
                     ALRSKNPPRMAGRCSVFAKTDMIHLQQVATPNYDIVAGLCFAMARNFKATVAQGKKIT
                     KPVSFQGGVAANLGMVRAFKEVLELKDDELIVSFYYAHIGAIGAVLSIMEQENIPQPN
                     LDMQLLDEYLTSTTSTFKSQSKLAFDFPENKYYSPAKNHLSQTDKIIKGFLGIDVGSL
                     STNLAVLDESKNVLVSCYLMTEGRPIDAIKKGLGIISQEIGNGVEIAAVGTTGSGRYL
                     TGDFVGADIVQNEISAQATAAVNIDENVDTIFEIGGQDSKYISLDKGIVVDFEMNKAC
                     AAGTGSFLQEQAEKLNIRVEDFGDIALTSFAPAACGERCTVFMESDITAHQQGGRKKE
                     DLTAGLCYAIVHNYLNKVVGKRKIGNNIFFQGGVAWNKGVVSAFETVLGKRITVPPHH
                     DVTGAIGAAILAMQNHRGGRSKFKGFELSERKYSVLTFGCNDCPNNCEIKEVKVENEK
                     PLYYGSRCEKYELKKSQTRKDKPLDLFEERNKILFSIHKGFAKPSRNRRERIGIPRTL
                     FFYELFPLWATFFENLGFEVVLSDKTNKTIVHQGIEKVLAETCFPIKVAHGHILNLLE
                     KNVDFIFLPSLISMPKKNDIFPESYACPYVQALPYMIKATLPVAEKLLSPSISLSLSE
                     ELVLRELCKLRRKLNIKTKDIKIALQRSFSCQEEFYRKIRELGSEALNKLDKRGLVVV
                     SRTYNSCDAYINLELSKKLNELGAVAIPIDLIRDVSDEAPIPNMYWKYGQDILATTKF
                     IRENPLLYPIYLTNFGCGPDSFITHFFRKHLKNKPYLQLELDEHSADAGIITRCEAFL
                     DSTRNRKYSMEKTYPQRRVVSKTGLQNRILYIPYMADHAFVLKSALSVWGIEAQVMPP
                     SNEETLEQGRKFTSGKECYPCILTTGDIVKFVQGKEFDSKRSAFLMPKADGPCRFGQY
                     HNLHRMVLDELGLQEVPIISPTSKNSYAEFGNLDGDFRKLSWAGIVACDLLLKMARHF
                     RAYEIVRGETDKVYNNLLKKLCDTIEKRGDLTLFLKKAKAEFGKIEIYRNIQKPIIGI
                     VGEIYIRSNPFSNNFLEGKIEELGGEVWLAPFGEWISYTTYMYGYHSLNRKDYVEYLK
                     ACAKETIQKRIEHSLCQAAKDGFGGMEDAPVDRLIRLAQPYLDRSFGGEAILSIGKSI
                     DYIRQSLCGIINTMPFTCMPGNVTCALSRKVQEDFGNFPWLNMAYDGLEDNSQLTRLE
                     AFMHRALEYNRSEQKRGGKSESKVQ"
     gene            11251..12879
                     /locus_tag="VGB16_04860"
     CDS             11251..12879
                     /locus_tag="VGB16_04860"
                     /inference="COORDINATES: protein motif:HMM:NF017745.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF885 domain-containing protein"
                     /protein_id="HEX9917048.1"
                     /translation="MNQKFNNLVDAILEFRWMTSPLEATLMGIHKYDERLDQVDRKTR
                     QNYTQKLESYVEKLDEFKESDLSKDEQLDWELLSNSLRAEIRIEQGFNRYERDASLCP
                     EITLWGCYILMMRDFAPIEKRMKSVLARMEQIPRLLDQAKENLKKGKNIPPIWTQLGI
                     EMATNGKQFFLILLPEFAKKVPQLKDALLSANQKVVTALDDYVAFLKEQILPKSNGDF
                     KLGRELFDFMLKTHHQLSYNADELLEIGHKLIQQTQEELQKLSASIDPKKSWVEIIAE
                     LKKSHPPKNKLLDFYTAEMNRAKDFVKNKDLVTIPPGENLSVMETPPFQRNVIPYGAY
                     VSPAPFEEKQEGFFWVTPVNENLPPEQQEEQMEGHNTFGAVLTALHEAYPGHHLQLVH
                     SNRVNSKVRRQFGTSLFAEGWALYCEEMMYEQGFYTDPRTRLHQLKDQLWRACRVVID
                     TSLHTGKMNFDQAVDMLIDVAKLEKTNAIAEVKRYTQTPTQPMTYCLGKMEILLLREE
                     VKKKGPLNLKDFHNQLLSFGTIPVRMVRKRMLEN"
     gene            13115..13255
                     /locus_tag="VGB16_04865"
     CDS             13115..13255
                     /locus_tag="VGB16_04865"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_012034180.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF5679 domain-containing protein"
                     /protein_id="HEX9917049.1"
                     /translation="MQAYCLKCKSKKEMKDEEQVTLKNGRKAIKGKCPACGTGMFKIL
                     GK"
BASE COUNT         4018 a         3044 c         2930 g         3939 t
ORIGIN      
        1 aagctttcta ctctatcacc tcagttatca ctcctgcgcc tacagtcctg ccaccttcac
       61 gaatagcaaa ccttagttcc ttttccatgg ctatgggggt aattaagtcc acctcgaagt
      121 tggcgttatc tccaggcata accatttcca ccccttcagg caatttaacc acacccgtaa
      181 catccgtggt gcggaagtag aactgaggac ggtatccgtt gaagaagggg gtatgtctgc
      241 caccctcttc tttggtgaga acatacactt cgcttttgaa cttggtatgg ggggtgatgc
      301 tgcctggggc ggctatgacc attcctcttt ctaattcacc tcgctctacc cctcttaaga
      361 gtaagccaac attatctccg gcttgggctt ggtctaagag cttgcggaac atctccactc
      421 cagtgaccac cgtctttttg gtctccctta tgcccaccct ctccacctca tctcccacct
      481 taatggctcc acgttcaacc ctgccagtgc ccacagttcc tcttccagtt atgctgaaaa
      541 catcctccac tggcaagagg aagggcttat ctgtttccct tttgggctgg gggatgtagt
      601 tgtccatggc agccagtagc tcgtagatgc acttgaagga ggggtcatcc ttaacttccg
      661 acttggctgc ttgggtcata gcttctaagg cgcttcccct tatcatgggt atctcgtctc
      721 cggggaactg gtatttcttt aagaggtctc ggacttcaag ttcaactaag tccaagagct
      781 cagggtcatc caccatatcc actttattca agaagacgat gatgtaaggc actcccacct
      841 gccgagccag aaggatatgc tctctggtct ggggcatggg tccatctgcg gctgagacca
      901 ctaaaaccgc tccatccatc tgagctgctc cagtgatcat attcttgatg taatctgcat
      961 ggcccggaca atctatatgg gcgtagtgtc ttttttcagt ctcatactcc acatgggcaa
     1021 ccgctatggt tattccccgc tccctctcct ccggagcgtt atcgatgcta tcgaaggtgc
     1081 ggacgtctgc catattctta cgatgcaaca ccatggttat agctgcagtc aacgtggtct
     1141 taccatgatc cacatgtccg atggtaccaa tgttaagatg gggcttggtt cgctcaaaac
     1201 gcttcttgga cataaaacct ccctttacaa attatctttt ttatcttcat acagcccagg
     1261 accaggattg gactggtggc cttccgtatg gatgctctac caatcaagtt gcgagggcaa
     1321 ttactcaatc ccttttatta ttttcaattt ctccactata ccatccttac tcttcaaccc
     1381 tctttccttt ttcatggctt cagcccgagc gttgaattct tcacagtaaa ttatctccca
     1441 tggctgaaat ctgcttgtcc aatgcttctt tcgattgtta tgttctgtca atcttctctc
     1501 aaggtctttc gttaaaccaa cgtaataacg atctactatt ttacttttga gaacataaac
     1561 gtaatatttc acctttcttc taaagcccag gaccaggatt gaactggtgg cctcatcctt
     1621 accaaggatg tgctctacca actgagctac ctgggcaaac tcccaatcgt taattcatct
     1681 cataaatctg aactggtgac cttccatatg gatgctctac caactgagct acctaggcaa
     1741 atagggatat aattctcaat ttaaggaaac aacaaaccat taatataaat ctatcaaaat
     1801 aattgtcaag cgaaaattta tgaaaattgc aacttttttg gacgaatata tccagcgacg
     1861 tatcaattca acttcctaat aatctttgtc ttatgacttt cttcgtccgc agaaaagcct
     1921 tggccctatg gctgattcga ttcttctggc ttgcgctcat ttgaccgaaa gttttctttt
     1981 ccggaggaaa gtaaaaaacg ggatcatagc caaacccctg atccccacat tccttatcgg
     2041 caacataccc aaaaactttt ccctcatcta tgaaaatctc tttattgttt agaaccaatg
     2101 ccgcaacgca aacgaaagtc gccctccttt tcttccaagg cacgccttcc agcaaagaca
     2161 ataacttgcg gttgttatct gtataagtgc aattctcacc tgcgaagcga gctgaatcga
     2221 ctccgggtgc gccatccaga gcgcttactt ctatacctga atcatctgcc aacgagggta
     2281 aaccggtgaa atcgaacacg gaccttgctt tgaggattgc attctctttt aagctcgttc
     2341 cggtctcttt gacttcgggc aaaccaggca actcctccaa acttaagagc tcaactttca
     2401 aatcccccag aatctctttt atctctttaa tcttattctt gtttcttgtg gcaaggacta
     2461 ttcttgtcat caatacctgt cgatttctat tctttggact cgtcttatct tcctgccgag
     2521 gaatctctca cccacttgca cgaacttttc cggcacatct gaaacgaaga aagcgtgagc
     2581 tcccctctca tttgacctag ctgtaaccct ttttgccttg aggcgtctct ttaactcaaa
     2641 agctatctct tcagccgaat cgatcagttt gaccttatta cccataaccc gggcaatgac
     2701 tcccttaagt aaaggataat gggtgcagcc caagatcaag gtatcaattc tttttctcac
     2761 aagaggcttg agatattcct gaacaatcat agttgtggct ttcttgttta tatacccctc
     2821 ctctgccaaa gctaccaaca acgggcaagc gcgagtgaag actcttatat ttttgtctaa
     2881 cttcctgatg gctttggaat aggatttcga agctatggtg ccaactgtgc cgattactcc
     2941 taccctctta ttttttgtac acctgacagc tgttcttgct ccgggttcaa tgactcccag
     3001 cataggggta ttgaattttc ttttcaaatg gtccaatgca aaagcagaag cagtattgca
     3061 agccactacg atgaaatcaa ctttctttga taaaagaaac cgagtgtttt gataagaaag
     3121 cttttgcact accttagacg agcgtggccc ataagggtat ctggctgtat ccccaaaata
     3181 aaccacatcc acctgcggga gaagctcgaa aatccgagcc gcaacagtta accctccaat
     3241 tcctgaatcg aatataccta tcttgatttt catttttttc ctttggtctc atatttctgt
     3301 ttgaatcttt ttatactttg atacaaagat ttagcgatct cgtcgcgaac agagtctttt
     3361 ttcaacagct tctcctcctc tttattgctt atgaaggcta cttccaccaa aatcgccggc
     3421 atataggtct tgtttaagac tacaaattcc gcttgatcca ctccacgatt ttccctcttg
     3481 ctttttctat ccagctcttg ttgaatgatg gaagccaatt cgcttgattc tttcaagaat
     3541 tcattctgca ccatatccag tagtatgaag tccaaatcgc ttatctcctt ccgtgcctct
     3601 ttgggcttct caaattgcag agaggcgttc tccagagttg ccactgcccg cgcctcatca
     3661 ttttttgact gagctaagaa aaaggtctca aatccagtgg cagtttttct gggagcggaa
     3721 ttggcgtgaa tacttatgaa aatatccccc cctgcccgat tggcaattct cgacctctcc
     3781 tccagagcaa cgaagacgtc ttctcgtcga gttagcatca cttttaaacc cttctcttcc
     3841 ctcagatacc ttgataattt cttagcaatg tccagagtta catctttttc aactaagccc
     3901 gatggtccaa ccgctcccaa atcctcacca ccgtgtcccg ggtctatcac caccacgtca
     3961 atccggttgt cttcgagatt ttctttttgc aacgacgact gtatggcaac agctgtcttg
     4021 gctgtatctt ccagggatac ttgcagacaa tagggattgg attgcatctg ctccagatgc
     4081 ttatcaaaac cctttgccat ctttaaggag agctgggctg cattatcgaa ctggtatgct
     4141 tttatctcgg ttatacattt gggtattgtt cttccggaaa aaaatgcggt gtccaatttt
     4201 ccttcataaa aattgacatt cacccatctg gagtcaccca ggaaaagatg atattccaag
     4261 ggctcagaca ggaatatttc gattagaatt ccgtttgttt tctggctagc tcttatatcc
     4321 aaaatattca tcttgcttgg gttgaaggac aatttccttt cctttttgcc ccagcttatc
     4381 tcctgcgaaa ccaacctgcc cagaatcggt ttaaaagtct caagcggaac atagattgtg
     4441 ccttttttga actcaatttc gcgcaccaga ttataaactg tggtatcgac cactatgtag
     4501 ggcgagaatg gcgagaggat taattcatgt ccattaaact gccaggttcc ctttttggtc
     4561 aattgattcc aggaaacttc cccttttaaa acctcattta gctcggtgat ggaaacatat
     4621 gttaagttga acttctcgta tgaatctata cgaagagact ttccccctat ctccatatct
     4681 actgaggata gggcatagga gataatacag accgagatga ttgaaaagaa aagcagaaaa
     4741 gacaactttc gattcatcgg tttttctctc tgaaagctct ttgcatttcc ctttgcatat
     4801 ctctttcttt gatatctctc tttctgtcgt ataccttttt tcctttagct aaagccaatt
     4861 caattttagc caccttccct ttaaagtaaa gcttcaaggg aatcaaagtc agacctctct
     4921 ccgctacttt gccgaaaagc tttctcatct cttttttatg caaaagcaat tttcttggac
     4981 gagttgggtt cagattatac ctattcccct gctcataagg gcttacatgc atatttaaaa
     5041 cgaaaagctc cccattttta aatatggcaa aactgtcttt taaattcgcc ttgccagaac
     5101 gtaaggattt cacttccgtg ccaaccaaaa ccaaacctgc ctcgagactt tctaaaacct
     5161 cataaagata atgtgctttt ctgttggtgc aaaccacttt tgtattttct tcttcctctt
     5221 tcatacgggg gaatatatca aactggggct tggcttgcaa ggaaattctg ttgttttgcc
     5281 aagaacatga atcagggcag gcacaggggc ctgcccctac aaaaaccatg ttgttattgc
     5341 gagataaaac tgcacagttc atattgagga gtcctgatgg taatcagatt accgaaaagg
     5401 tcgaacgggg aaaggtttta tggagtgtca agcttcagct tgacctgaaa tcttttgaag
     5461 gtgaacactc caaagtctcc tttgttggtt cgggaattgg aattttccgg ctttagcgga
     5521 atcctggcga cccgaaccat aaaattgtgc aggaaatccc cggcacgggt cttgacgaca
     5581 attcctgcac aaacggagaa atcatctttt tatatcctct ccctcgaagg gagaggatag
     5641 aggtgagggt gaaattttga aattgactta cttcgagcaa agaattatat tccgtttgtc
     5701 gataaaagtg aagcttcctc gtgacaaatc acggagcttc ccaaatataa aaagcgaaac
     5761 ttcgccgaag ctcgcctcgt catttctggt ggaggcgagt aaaaatgccg aagtggtgga
     5821 attggcagac acgctgtgtt cagggcgcag tgtccaaaag acgtgcgggt tcaaatcccg
     5881 ccttcggcat aagatttgtt tagcccccta ggagttaaat cgtacaatga atataagagg
     5941 ggttattgtt tcttttctaa aaacaatatt cgaatttata gtacgcatcc taaaatttct
     6001 tttagcaaaa gtgcttcttc caatttggag attcattaca aaaaatgccc ctcaggttca
     6061 agcattgatt gctgttttag ctttagggct actcgcatat aacattactc tttctattaa
     6121 gaaccggaat gatagtcgca aattcggaca acagatgaaa ggttatgctg aggacatcag
     6181 agcatcactc gcagaaacta acaaaggatt aaaaatttta ccttcaagtg tcgagagttt
     6241 ggattctgct gttatcaaag tgacttccac tgtcgacaga atatcaatta accaagataa
     6301 gtttgcaata gccacgaaaa cattagaaca gaatgttacc ctctttggtg acaagcttgc
     6361 ggaagttata gaagcaaaca aagaacaatt gaaaatatgg agcgatagca gaaaggcgtt
     6421 agaggaagaa ttgcacaaag tccctgaatt agaaatattt agcaaaccta tttcttacaa
     6481 ccaaaaagat tctacttact ccatgagtgt gtggatcagg aataagggaa aaagagcagc
     6541 acgtggattt tcggtattct tatctataga tccgaagata aacgcacgcg cctataacga
     6601 aggcgtttgg gttgtggaag aacgagtagt agaacacaag gaatggattg aatgcaacca
     6661 tatggagaga gaaaaaccag tttggccaga cagtaactta agcatcaatg tggcagcttt
     6721 gagattcaag ttaggggact cgaatatgaa acaatttact gtgtattggc aggttggaca
     6781 tgaccttgcc accaattccg gcaaatttaa aatagatatc cctacaaact aagtctgcaa
     6841 tttcgtaaga taggtgagct ctgcaattca aatgaaattc tttccatccc ctctcccttt
     6901 atgggagagg gttagggtga gggtgacgta ggggcaattc atgaattgcc cctacaaata
     6961 gaataacaaa aatgtaatgc ggtctagttg ggtcgcctta catttttccg ccataaattc
     7021 ctttacaaac cccccttatc tccgtataat aaatatctat gaaaggaaat atatttttag
     7081 gcatcgatgt tggctcaatt gcggtcaagg ttgttttaat ctctccaaac aaagagatac
     7141 ttaagacctt ctacgttcgc tcccagggac aacctctctt ggtcgtcaag aatgcctttt
     7201 tggaaatatt caaagaatat gatagggaaa atatcttagc tcttgccaca accggctccg
     7261 gcgggaaaac catttccagg attttggatg gatatttcgt caacgaagtt attgcccagg
     7321 tcaaaggcac aggatatttg cttcccaagg ttagaaccat aatcgaaatc ggaggggagg
     7381 actcaaaatt tatcctcttg cattatgatg aagaagctaa aacctccatt ttggacgact
     7441 ttgcaatgaa tagcatctgt gcggctggaa ccggctcatt tttagaccag caggcaaata
     7501 gattaaaaat atcaatcgag gatgaatttg gccaattagc tttgaggtca aaaaacccac
     7561 cccgtatggc tggaagatgt agtgtctttg caaaaacgga tatgatacac ctgcaacagg
     7621 tcgccactcc gaattacgat attgtggctg gtttatgctt tgctatggca aggaacttca
     7681 aagcaacagt tgcccagggc aagaaaatta caaagccggt ttcctttcag ggtggcgttg
     7741 cagccaattt agggatggtc agggctttca aagaagttct tgaattgaaa gatgatgaac
     7801 tgattgtgtc tttttattat gctcatatcg gagcaatcgg tgctgttctc tcaattatgg
     7861 agcaagagaa tattccccag ccgaacctcg atatgcagct tctggatgag tatcttactt
     7921 caactacatc caccttcaag tctcaatcta aacttgcttt tgatttcccg gaaaataaat
     7981 attactcgcc agctaaaaat catctaagcc agactgataa gataataaag ggttttctgg
     8041 ggatagatgt cggctccctt tcaaccaatt tggcagttct ggatgaaagc aaaaatgtgc
     8101 tggtaagttg ttatctaatg actgaaggaa ggccgattga cgctataaaa aaggggctgg
     8161 ggataatcag tcaagagata gggaatggag ttgaaattgc cgctgtgggg acaactggct
     8221 cgggcagata cctgaccggt gattttgtgg gagcggatat tgttcagaat gagataagtg
     8281 ctcaagccac tgccgcggtg aatatagatg aaaatgttga taccatcttt gagataggtg
     8341 gccaggattc caaatatatt agtctcgaca agggaatagt agtcgatttt gaaatgaaca
     8401 aggcttgcgc tgccgggacc ggctcatttc tgcaggagca ggcagagaaa ctgaatatca
     8461 gagtagagga ttttggagat atagccctca cttcctttgc tccggcagct tgcggtgaac
     8521 gttgcactgt tttcatggaa tcggatataa ccgctcacca acaaggcggc agaaaaaaag
     8581 aagacctgac agcagggctt tgttacgcca ttgtgcataa ctatcttaat aaggtggtgg
     8641 gaaaaaggaa aattggaaac aacatattct tccagggcgg agttgcctgg aacaaaggtg
     8701 tggtcagcgc cttcgaaact gttttgggga aacggataac tgtccctccg caccacgatg
     8761 taactggtgc tatcggtgcg gctattttgg caatgcaaaa ccaccgaggg ggaagaagca
     8821 aatttaaggg gttcgaatta tctgaacgga aatattcggt tttgactttc ggttgcaatg
     8881 attgtccgaa taattgcgag ataaaagaag tcaaggtgga aaacgaaaaa cctctatact
     8941 acggcagccg ctgtgaaaag tatgaattga aaaagagcca gacaagaaaa gataagccgc
     9001 ttgacctgtt tgaagaaaga aacaaaattc tttttagcat acataagggc tttgccaagc
     9061 catccaggaa tagaagagaa agaattggaa ttcctcgaac ccttttcttt tatgaacttt
     9121 tccctctctg ggcaaccttt ttcgagaatt tgggatttga agttgtcttg tctgacaaga
     9181 ccaacaagac catcgtacat caaggaatag aaaaggtcct ggctgaaacc tgcttcccta
     9241 taaaagtagc tcatggtcat attttgaacc tcttagagaa aaatgttgat ttcatctttc
     9301 tgcccagctt gataagcatg cccaagaaga atgatatttt tccggagagt tatgcttgtc
     9361 cctatgttca agctctacct tatatgatta aagcgacttt gcctgttgca gagaaactcc
     9421 tctccccttc catctctttg agcttaagcg aagagcttgt gctaagagaa ttgtgtaagt
     9481 tgagaagaaa actaaacatt aaaacaaaag atattaaaat cgctcttcaa agaagtttct
     9541 cctgtcaaga agagttttac agaaagataa gagagcttgg cagcgaggcc ctgaacaaac
     9601 tggacaagag ggggttagtg gttgtgagcc gaacgtacaa cagctgtgat gcttacatca
     9661 acttagaact atccaagaaa ctgaatgaac tgggggcagt ggcaataccg atagatttaa
     9721 ttagagacgt atcggatgaa gcgccgatcc cgaatatgta ttggaagtac gggcaagata
     9781 tcttggcaac aaccaaattc atccgagaaa atccccttct ttatccgatt tatctaacca
     9841 attttggctg tggacctgat tccttcataa ctcatttctt caggaaacac ttgaagaata
     9901 aaccctatct tcaattagaa ttggatgagc attcagctga tgctggaata ataacccgct
     9961 gtgaggcctt cctggatagc acaagaaatc gcaaatatag catggaaaag acataccctc
    10021 aacggagagt ggtttcgaag acgggcttgc aaaatagaat cttgtatata ccttatatgg
    10081 cagatcatgc ttttgttttg aaatctgctc tttcggtctg gggaatagaa gcacaagtta
    10141 tgccaccgtc aaacgaagaa actctggagc aaggaagaaa gttcacctct ggaaaagaat
    10201 gttatccctg tattttgact acgggtgaca tcgtcaaatt cgttcaagga aaagagtttg
    10261 attcaaaaag gtccgctttc cttatgccta aagcagatgg tccttgtcgt tttgggcagt
    10321 atcacaatct acatcgcatg gtcttggatg agctgggact gcaagaagtg cctataattt
    10381 ctcccacctc aaaaaactct tatgctgagt ttggaaatct ggatggagat ttcagaaaac
    10441 tgagctgggc tggaattgtg gcttgtgacc ttcttttgaa aatggctcga cacttcaggg
    10501 cttacgagat agtcagaggg gaaacggaca aggtctataa taatctttta aagaagctat
    10561 gcgacaccat tgagaaaaga ggagacttga ctctatttct caagaaagca aaagccgagt
    10621 ttgggaagat agaaatttac cggaatatcc aaaaaccgat tatcggaatc gtcggggaaa
    10681 tttacatccg ttccaatccc ttctcgaata acttcctgga ggggaaaatc gaggagctgg
    10741 gtggtgaggt ctggctcgct ccgttcggag agtggatatc ttatactacc tatatgtatg
    10801 gataccatag tctgaatcgt aaagactatg ttgaatatct taaagcttgt gccaaagaga
    10861 caatacagaa aaggatcgag catagcctct gccaggcagc caaagatgga tttggaggta
    10921 tggaagatgc gcctgtggac agactgataa ggctggctca accatatctg gacaggtctt
    10981 ttggaggcga agcgatcctt tccattggaa aatcaataga ctatataagg cagagtttat
    11041 gtggaatcat aaacaccatg ccttttacct gcatgcctgg aaatgtaact tgcgccttat
    11101 ccaggaaagt gcaagaagac ttcggcaatt ttccttggtt aaatatggct tacgatggtt
    11161 tggaggataa cagtcagtta acccgtttgg aggcatttat gcatcgagct ctcgaatata
    11221 atcgttcgga acagaaaaga ggaggtaaga gtgaatcaaa agttcaataa cctggtggac
    11281 gcaattttag agtttcgctg gatgaccagc ccgcttgaag ccacccttat gggtatccat
    11341 aaatacgatg agaggcttga ccaggtggat aggaagacca gacagaacta tactcaaaag
    11401 ctggagtctt atgtagaaaa gttagatgag ttcaaagaaa gtgacctgtc caaagatgag
    11461 cagctggatt gggagctcct gagtaattca ctgagagctg aaataaggat cgaacaagga
    11521 ttcaatcgct acgaaagaga tgcttccttg tgcccggaaa taactctgtg gggatgttat
    11581 atcttaatga tgagagattt tgccccaata gaaaagagaa tgaaatcagt cttagccaga
    11641 atggagcaga ttcccagact attggaccag gctaaggaga acttgaaaaa gggtaagaat
    11701 attcccccaa tctggactca actcggaata gaaatggcga ctaatggcaa acaatttttc
    11761 ttgattcttc tgcctgaatt cgcaaaaaag gttccccaat tgaaggatgc tcttctttca
    11821 gccaaccaga aagtggttac tgctttggat gattatgttg cattcctaaa agagcaaatc
    11881 cttcccaaat caaatggaga tttcaaattg ggcagagagt tgtttgattt tatgttaaag
    11941 acacatcatc aactttctta taatgcagat gaactattgg aaatcgggca taagctcatc
    12001 caacaaaccc aggaggaact tcagaagtta tctgccagca ttgacccgaa aaagagctgg
    12061 gttgagataa tcgccgaact taagaaatct catcctccta agaataagct tttggatttc
    12121 tataccgctg agatgaatcg ggctaaggat tttgtcaaaa acaaagattt ggtaacaatt
    12181 cctcccggag aaaatttgag tgttatggaa accccgccat tccagagaaa tgtgattcct
    12241 tatggagcct atgtttcccc tgccccgttt gaggaaaaac aggaaggttt cttctgggtt
    12301 acgcccgtaa acgaaaacct tcctccggaa caacaggaag agcaaatgga aggacacaac
    12361 acctttggtg cggttctaac cgccttacat gaggcttatc cgggccatca cttgcaattg
    12421 gtgcattcca atcgagtgaa ttccaaggtc aggcggcaat tcggcacctc cctttttgct
    12481 gaaggatggg cgctttactg tgaggagatg atgtatgaac aggggttcta tactgaccct
    12541 cgtacccgct tgcatcagct caaagaccaa ctctggcgcg cctgtcgagt ggtaattgat
    12601 actagtctgc ataccggaaa aatgaatttt gaccaagcag ttgatatgct aattgatgtg
    12661 gctaaattag aaaagaccaa tgccatagct gaagtcaaaa gatatactca aacccccacc
    12721 caacctatga cctattgctt aggtaaaatg gagatattgc tacttagaga agaggtcaag
    12781 aaaaagggac cgttgaatct aaaagatttt cataaccagc tgttaagctt cggcaccatt
    12841 ccagtaagga tggtgagaaa gaggatgctg gagaattaat ttagtttgga ctggttgaca
    12901 taaatgtacc ccacctaaac agaagttggg ttgcttagaa cagagacagg cgggtggccc
    12961 acagctctgc tgtggggcac ctgacaaaat aactaaaccc ctggcccgtc tctgaagcat
    13021 gcaaggatag cgtgttaagg agtgcttgtg agcattgttt atcaaaggtt catttataat
    13081 ttaaaaacaa caaaatggca gaaaaggagt taacatgcaa gcatactgtt tgaaatgtaa
    13141 atcaaagaaa gaaatgaaag atgaagaaca agtaacttta aaaaacggca ggaaagcaat
    13201 aaaaggcaaa tgtcctgcct gtggcacggg catgtttaag atattgggga aatagcttgt
    13261 aagggcaatt catgtggaac atctcgctaa aagcgggaaa ttgcccctac tatgttttgt
    13321 catccccgag ggtttaatcg gggatccata ctcgcttttt ttattctgga tttcccgtat
    13381 ccagtacggg acaagccctg ctgcagttta tcccgcagaa atgcgggaca ggaatgacac
    13441 aaaaactcac aaattgcttt aatcccgatc ttacaggtgg gaagttcccg cggggttaat
    13501 attgattttc tttcctctcg cttcccaatc cccatacaaa cctcttaaat tctcctgatg
    13561 ttacttctta atggagtacc aaaaccatta caaaagtgca tttccttcct atttagatga
    13621 ctccttatta aacccgcgcc ctttctatca tataggcacg gataagattg cttccaccca
    13681 aaaaatacct tatatataat cccttagttc tcacgaataa accaattcca ctaatctcct
    13741 ctttcacaaa ctaatctcct ctcccctttc tgctgaatta aaggaatagg tgaggtacta
    13801 agtaagatat tgacatcgga gagtttgggc tataaattta ttattgcttc acataagctt
    13861 gtaatgatac aactcaggcg aagttttgcc tggagtatta tctggaagcg aatccagaaa
    13921 gtgggtttat g
//
LOCUS       DASWLN010000144         4028 bp    DNA     linear   ENV 20-FEB-2024
DEFINITION  MAG TPA_asm: Candidatus Zixiibacteriota bacterium isolate
            SMAG_U2949 k141_753110, whole genome shotgun sequence.
ACCESSION   DASWLN010000144 DASWLN010000000
VERSION     DASWLN010000144.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37960594
            Sequence Read Archive: SRR10376091
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Candidatus Zixiibacteriota bacterium (soil metagenome)
  ORGANISM  Candidatus Zixiibacteriota bacterium
            Bacteria.
REFERENCE   1  (bases 1 to 4028)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 4028)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (26-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/12/2024 08:28:26
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 1,440
            CDSs (total)                      :: 1,416
            Genes (coding)                    :: 1,412
            CDSs (with protein)               :: 1,412
            Genes (RNA)                       :: 24
            rRNAs                             :: 1 (16S)
            partial rRNAs                     :: 1 (16S)
            tRNAs                             :: 21
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 4
            CDSs (without protein)            :: 4
            Pseudo Genes (ambiguous residues) :: 0 of 4
            Pseudo Genes (frameshifted)       :: 0 of 4
            Pseudo Genes (incomplete)         :: 3 of 4
            Pseudo Genes (internal stop)      :: 1 of 4
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..4028
                     /organism="Candidatus Zixiibacteriota bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_753110"
                     /isolate="SMAG_U2949"
                     /isolation_source="soil"
                     /db_xref="taxon:2053527"
                     /environmental_sample
                     /country="USA: Oregon"
                     /lat_lon="42.99 N 123.63 W"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            <1..1404
                     /locus_tag="VGB16_04870"
     CDS             <1..1404
                     /locus_tag="VGB16_04870"
                     /inference="COORDINATES: protein motif:HMM:NF013069.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0016020 - membrane [Evidence IEA];
                     GO_function: GO:0022857 - transmembrane transporter
                     activity [Evidence IEA];
                     GO_process: GO:0055085 - transmembrane transport [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="efflux RND transporter permease subunit"
                     /protein_id="HEX9917050.1"
                     /translation="GTDLEHTSQVLAQIEKIVRDNVPEVETFFLRAGVEGKGFGAMTV
                     LFSDITGPHSALIRVELVSAKQRKRSVFEIVELLRSKTDHIPDADIKYNPSGFFSRIG
                     AFGATAPVLVEVRGYDLATSSALSQKVAGIVKSIPGSRDVKISREEGLPEYEIQIDRD
                     KASALGLNLSQIASTIQTQVGGKVASLYLDPKLGKEFEISVRLREADRRTLLDLDQIF
                     IGTPSGKQVPLSNLAKVTKTKGPVKIDRKNQERFVSISCQVSDRPAGDVARDVESRIK
                     KEISIPEGFSINVAGSYQEQKDSFKVLLFALLLAVALVYMVMAAQFESLLDPFIVMFS
                     VPLGIIGVIWGLFFTGKTLSTLSFLGIIMMAGIVVSNAILLVDYTNVLRKRGLELEEA
                     VIKAGRTRLRPILMTSLATVMALIPMALGIGEGAELWSPMGVTVISGLLVSMFLTLVL
                     IPTLYAMVNKKAISQGA"
     gene            1787..>4028
                     /locus_tag="VGB16_04875"
     CDS             1787..>4028
                     /locus_tag="VGB16_04875"
                     /inference="COORDINATES: protein motif:HMM:NF012298.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004672 - protein kinase activity
                     [Evidence IEA];
                     GO_process: GO:0006468 - protein phosphorylation [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="protein kinase"
                     /protein_id="HEX9917051.1"
                     /translation="MIGKTISHYKILEKLGEGGMGVVYKAQDTKLDRIVALKFLPKHL
                     LCDSEAKARFIHEAQAASALNHPNITTIHEIDEIEGECFISMEYIEGRDIKELVEKKG
                     LSIEEILKIAIQVVEGLSRAHQKDIVHRDIKSDNIMVTKDGLVKIMDFGLAKLKGATK
                     LTTTGSTLGTAAYMSPEQAQGIEVDQRSDIFSFGVVLYEMITAQLPFKGEHEAAIIYS
                     IVNETPEPLARYKANVPEGLQRIVDKALKKDRNIRYQSAADMTADLKGLQKETTGVSV
                     VKPKKKILSFIVPASVVFILVLLFLILKPFKFEISPEKKAAAAENTLAIMYFDNLVDP
                     ADPTRLGEIITNLLITDLTESRYLRVVSSQRLYDILKLLGKEGIKRVDRAIATQVAEK
                     ANAKWMLLGSVLQVKPEIILTAQLVEVKTGNAIASQRISGQAGENIFPLVDRLTVEIK
                     KDLSLPSMAQKEPDPLVADVTTHSPEAYRYYLEGVDYYNKIYFKEAEQSFRKALEYDS
                     TFAMAYYQLAKLDWNKEFIAKAVKYSKNAGYKEKCYIKAYYEAAYVSGGSSKYIKELQ
                     KIVERYPEEKEAFFRLGLFYDHSRLFEEAVYYLNKAIEIDPLYKLAYNELAYAHSGMG
                     DFEKSIWAINKYISLAPNEPNPYDTRADLYAYNGKLDQAIESYKKALEIKPDFYGSLE
                     KLGHMYLFKREYPKAESCYRVFSVSDDKDERAMGRTCLSLIPLYQGEFEDALKILDDG
                     LTADRME"
BASE COUNT         1230 a          732 c          962 g         1104 t
ORIGIN      
        1 gggactgact tagaacatac aagtcaggtc ttagctcaaa tagaaaagat agtaagagat
       61 aatgtgccgg aggttgaaac tttctttctg agagctggag ttgagggaaa gggatttgga
      121 gctatgaccg ttcttttcag tgacataacc ggtccacatt cggctctcat aagagttgaa
      181 ctggtaagcg ccaaacagcg caagaggtca gtgtttgaaa tcgtggagct cttacgctct
      241 aagactgacc atattccgga tgcagacata aaatacaatc ccagcggctt cttcagtcgg
      301 attggagctt ttggagcaac ggctccggta ttggtggagg tgaggggata tgacttagct
      361 acttcatctg ctttaagcca gaaggtcgcc gggatagtaa agagtattcc gggaagcagg
      421 gatgtcaaga tcagccgaga agagggattg ccggaatacg aaatccagat agatagagac
      481 aaagcttcgg ctttgggctt aaacctctca cagatagcga gcacgattca gacgcaagtc
      541 ggcggtaagg tggcgtcgct ttacctcgat cccaaattag gaaaggaatt cgagatatcg
      601 gttcgcttga gggaagctga tagaaggacc ctgttggatt tagatcagat ctttattggt
      661 actccttcag gaaaacaagt gccactttcg aacttggcta aggtgactaa gaccaaaggc
      721 ccggtgaaga tcgacaggaa aaaccaggag cggtttgttt ccatcagttg tcaggtatct
      781 gataggccag caggggacgt agcccgcgat gtggaaagca ggataaagaa agaaataagc
      841 attccggagg gattcagcat aaacgtggcc ggcagctatc aagaacaaaa agattcgttc
      901 aaagtccttt tatttgcttt gcttttggca gtggcgctgg tgtacatggt gatggccgcg
      961 cagtttgaat ctcttctgga tccttttatt gttatgttct ccgtgccttt gggaataatt
     1021 ggtgtgattt ggggattatt ttttactggc aaaacattga gtactctttc tttcttggga
     1081 attataatga tggcagggat tgtagtttca aatgccatac tcttagttga ctataccaac
     1141 gttttgagga aaagaggttt agaattagaa gaggcggtta tcaaagctgg cagaacacga
     1201 cttcgcccta ttttaatgac ctccttggcc acggttatgg cgttgatacc tatggctttg
     1261 ggaattggtg agggtgcaga gctgtggtct ccaatgggag taacggtgat aagtggactt
     1321 ttggtgtcga tgtttctgac cctggtcttg attcctactt tatatgccat ggtcaacaag
     1381 aaagccataa gccagggcgc ttaacaggtt gaataatcat tgaagttttg attaaagaga
     1441 attgcgttag tattttccct ttgggttggg cagttgccta actcattttt tgtatatccg
     1501 gaaaaaagcg ccactttttt ctcgtcactt tttatagata tagattcaga tataacgact
     1561 gcaagaatct cacagtattg atcaattttt caattgacaa ttcactattt ttaactaaaa
     1621 tggtgattga agctttcccg ggggtcaaaa tgctcggaaa gaccatctcc caccttttga
     1681 ggaatcgatt aaaggaaaga cttagagatt atgccggtag ccgcccattt gggcgcaaaa
     1741 atcttcgcag acataaagtc tgcggctaca gaatagaaga aaaaatatga taggcaaaac
     1801 gatttcacat tataagattc ttgagaagtt aggcgagggt ggtatgggcg tggtctataa
     1861 agcccaggat accaagctag ataggattgt ggctttaaag tttctaccca agcatctttt
     1921 gtgcgattct gaagccaaag ctcgatttat tcacgaggcg caagcagcct cagcccttaa
     1981 tcatccgaat atcaccacaa tccatgagat agacgaaatc gagggtgaat gtttcatttc
     2041 tatggaatat attgagggca gggatataaa agaattggtc gaaaaaaagg gtctatctat
     2101 agaagaaatc ttgaaaattg ctatccaagt agtagagggg ttatccagag ctcaccagaa
     2161 ggatatagtt caccgagata ttaagtctga caatataatg gtcaccaagg atggtttggt
     2221 gaagataatg gatttcggct tggccaagct taaaggtgcc accaagctga ccacaactgg
     2281 ctcgaccttg gggactgcgg cttatatgtc cccagagcaa gctcaaggaa tagaagtaga
     2341 ccagcgttca gatattttct cttttggtgt ggttttatat gagatgatta ccgcccagct
     2401 tccctttaag ggagagcatg aagccgccat aatctattct attgtaaatg agacccctga
     2461 accactggcc agatataaag ccaatgtccc agaaggctta cagaggatag ttgataaagc
     2521 cttaaagaaa gaccgcaata tcaggtatca aagtgcggct gatatgacag cagatttgaa
     2581 gggattgcag aaggagacca cgggtgttag tgtggtgaag cctaaaaaga aaattctgtc
     2641 ctttatagtt ccagcttcag tagttttcat cctggtttta cttttcttaa ttcttaaacc
     2701 cttcaaattt gaaatcagtc cagaaaaaaa ggctgctgca gcagagaata ccttagcaat
     2761 aatgtatttt gacaatttag ttgacccagc agatcctact agattaggag agattatcac
     2821 caatttatta attactgacc tgaccgagtc acggtatctc agggtagtat ccagccagcg
     2881 gttatatgat atcctcaagt tattgggtaa agaagggata aaaagggttg accgggcaat
     2941 agccactcag gtagcagaga aagccaatgc taaatggatg cttttgggta gtgttttaca
     3001 ggttaagccg gagataatac tgacagctca attagtagaa gttaaaactg gcaatgccat
     3061 cgcttcccag aggatcagcg gacaggcagg tgagaatatt ttccccttag ttgatagact
     3121 cacagtagaa atcaaaaaag acctatctct accttcaatg gcacagaaag aaccagaccc
     3181 tttggttgcg gatgttacta cccattcacc tgaagcatat cgttactatc tggagggagt
     3241 tgattactat aataagattt atttcaaaga agctgagcaa agtttcagga aagctttgga
     3301 gtatgattct acttttgcaa tggcatatta tcagttagct aaacttgatt ggaataaaga
     3361 attcatagct aaagcagtaa aatactctaa aaatgccggt tacaaggaaa aatgttacat
     3421 aaaagcatat tacgaggcag catacgtttc ggggggttca tctaaataca ttaaggaact
     3481 acagaagata gtcgaacgct atccagaaga gaaagaagca ttttttagat tagggctctt
     3541 ttatgatcat tcgcgactgt ttgaggaggc tgtctactat ttgaacaaag ccattgagat
     3601 agaccctctt tataaattag catataatga gcttgcctat gcccacagcg gaatggggga
     3661 ttttgagaaa tctatctggg caataaacaa atacatatcc ctggcaccga atgaacctaa
     3721 tccctatgat acaagagctg atctttatgc ctataatgga aaattggacc aagcaattga
     3781 atcttacaaa aaagccttag agataaaacc agatttctat gggtccttag aaaaactggg
     3841 gcatatgtat ctttttaaaa gagaatatcc aaaagctgag agttgctaca gggtcttttc
     3901 tgtaagtgat gacaaagatg aaagagcaat gggaaggact tgtttatctc taattccctt
     3961 ataccagggt gagtttgagg atgcgctcaa gattttagat gacggtctta ctgcagatag
     4021 gatggagc
//
LOCUS       DASWLN010000145         8045 bp    DNA     linear   ENV 20-FEB-2024
DEFINITION  MAG TPA_asm: Candidatus Zixiibacteriota bacterium isolate
            SMAG_U2949 k141_627917, whole genome shotgun sequence.
ACCESSION   DASWLN010000145 DASWLN010000000
VERSION     DASWLN010000145.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37960594
            Sequence Read Archive: SRR10376091
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Candidatus Zixiibacteriota bacterium (soil metagenome)
  ORGANISM  Candidatus Zixiibacteriota bacterium
            Bacteria.
REFERENCE   1  (bases 1 to 8045)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 8045)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (26-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/12/2024 08:28:26
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 1,440
            CDSs (total)                      :: 1,416
            Genes (coding)                    :: 1,412
            CDSs (with protein)               :: 1,412
            Genes (RNA)                       :: 24
            rRNAs                             :: 1 (16S)
            partial rRNAs                     :: 1 (16S)
            tRNAs                             :: 21
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 4
            CDSs (without protein)            :: 4
            Pseudo Genes (ambiguous residues) :: 0 of 4
            Pseudo Genes (frameshifted)       :: 0 of 4
            Pseudo Genes (incomplete)         :: 3 of 4
            Pseudo Genes (internal stop)      :: 1 of 4
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..8045
                     /organism="Candidatus Zixiibacteriota bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_627917"
                     /isolate="SMAG_U2949"
                     /isolation_source="soil"
                     /db_xref="taxon:2053527"
                     /environmental_sample
                     /country="USA: Oregon"
                     /lat_lon="42.99 N 123.63 W"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            complement(377..820)
                     /locus_tag="VGB16_04880"
     CDS             complement(377..820)
                     /locus_tag="VGB16_04880"
                     /EC_number="1.20.4.4"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_014163905.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0030612 - arsenate reductase (thioredoxin)
                     activity [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="arsenate reductase ArsC"
                     /protein_id="HEX9917052.1"
                     /translation="MGRKWKVLFLYTANSSRSQMAQGFLRSLAGNKYDAQSAGISPSI
                     LNPLAVRVMQEIGIDISKQNSKSVEVCLKEKFDYIITVCENARETCPNFPGGANKSYW
                     SFEDPANSSGDEDEKMKGFRKVRDQIAEKIRGFIKIAPEPPSRGR"
     gene            952..2127
                     /locus_tag="VGB16_04885"
     CDS             952..2127
                     /locus_tag="VGB16_04885"
                     /inference="COORDINATES: protein motif:HMM:NF018613.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016758 - hexosyltransferase activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="glycosyltransferase"
                     /protein_id="HEX9917053.1"
                     /translation="MPRKIVLAYVSAGAGHKSACMALQKAYLQLHPEDQVQVVDILKY
                     APWPFKTIYAEGYLLVANLHPTLWGWIYNKQVDIYKGKKEGIFSTWLNQFITKRFQKF
                     ITNISPDVVISAHFLSSWAAAEARKKNALNFVLGTVVTDYALHSAWLVKEQDLFFVST
                     EEIQWQLIKHKDYLGLDKKEIIVSGIPIDPVYAQKKDKNKLREKRNLQPDLFTILILG
                     GMAGSTKINDIISSILRIHLPFQLMMVSGANFCISAAHQKEMKKRGIPCVCFGYIDFM
                     DEVMQMADLALTKAGGLVCTECMSKGLPVVIFRPYPGQEERNTDYFLEQGAAMKVNQL
                     DSLDLKIIQLMENPEKLDKLSENALHIVNPSAALDIVKRIDERVQSKRAAAVNLSSL"
     gene            complement(2108..3538)
                     /locus_tag="VGB16_04890"
     CDS             complement(2108..3538)
                     /locus_tag="VGB16_04890"
                     /inference="COORDINATES: protein motif:HMM:TIGR04514.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="GWxTD domain-containing protein"
                     /protein_id="HEX9917054.1"
                     /translation="MLNRMTISLLAIGLLLCSCVSISIDTAYRKAWDRALPEHRIQYL
                     ELKPVMTEDDADSFLQMTDETQREEWLRKFWKAKDPTPTTERNEFKEEHQRRVAYARQ
                     YFGSHFRGNRLWDDRGEVYIKYGEPDERVTQVEGVYGVGKEEQEARKQEGEVWRYYKY
                     DLTLQFQDNKLLGFYELTPHVEVQSKDVYGETYEVQDEVDFKSTVATKIDIPREIYHH
                     DYEGDVLDYALDIVRFRNTGDNYEVDVNLGLPIEQLGKKDANYVSFVKSIVVFDNNYK
                     EVKRDSSIVNVRVGQAVNSLVIDQNRFVLNSGEYKVAVEVRDLVSSRIGIYKKDILLP
                     GYVHPEQREISQIVMATDVRKATQDDSLLMQRNGLVISPLPSRIYFADQKIYFYYEIY
                     DLKLDKSGKAYYIIQADLINTKTRKRTTLYKTNPVECTKSNTYEIDEIDALELKPGDY
                     ILTIKVKDVLADKEKNTVTAFRVMKD"
     gene            complement(3842..6223)
                     /locus_tag="VGB16_04895"
     CDS             complement(3842..6223)
                     /locus_tag="VGB16_04895"
                     /inference="COORDINATES: protein motif:HMM:NF012385.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_function: GO:0008134 - transcription factor binding
                     [Evidence IEA];
                     GO_process: GO:0006355 - regulation of DNA-templated
                     transcription [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="sigma 54-interacting transcriptional regulator"
                     /protein_id="HEX9917055.1"
                     /translation="MKKLSDPEKEKPLYELERLDLLLFEKKFKLALAELRELENQKVF
                     IEANSQSGHFYTIFSATLNNLGNHQEALEKGLKAFDILKHTNEIEKIVRIQTILGDTY
                     LRLGDLKNAQAEYRDSFAFYRSQEPCKKSLHLYNKLAHVCFVEGKFGEAIEYLRDGIE
                     CAKSLNDRNVLFQLGGNLGLVYVFLGKFELAEELRLLFKHPSMQQNEAFQIDWFLGYA
                     YVFMMRRKLADAEDYLDKSYDLILRHGATREKAIYHEYSGQLTFITGDLLSAERHYQE
                     ALKIGEEIAPEGDIISQTCRLLAELRFAQGNYDEASAFCQRSLGVCQKIKERFEEGIV
                     YRILGQVYASKGEFELAKENFEKGISILKEIGAKYELGKVYLEAGKSDCYSYFERLKL
                     LGQAEDALRDTDSKYLLAKNYLALADLLYENEQYEKGISFAKEAEKIFGESNEKEDLS
                     LAIRIRKKLESKAGQESIADKKSNLIFSNIVTQNRELLDIIKRMEQVLNTDLTILLEG
                     ETGTGKDLLAKTIHYNGNRKDKRYIALNCANLPETLFESELFGYKKGAFTGANLDKKG
                     LLEEVDGGTLLLNEIGEIPLSVQAKLLNVIEEKEFTRLGDTKPIKVNVRFIAATNQDL
                     KKAVEQRRFREDLYYRLSVIKVELPPLRERKEDVLPLCHYFLEKHCNGNCDKIETLSR
                     KIAESLFFYDWPGNVRELENEILKFSALMGTKQGSDLDRFIQQMRMEKLGPHQNGSIA
                     ERKVEMEIEEIGKALKVCNNIKASAAKMLDMPESTLRRKIKVYNISEVSEKKP"
     gene            complement(6764..>8045)
                     /locus_tag="VGB16_04900"
     CDS             complement(6764..>8045)
                     /locus_tag="VGB16_04900"
                     /inference="COORDINATES: protein motif:HMM:NF012235.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_process: GO:0015833 - peptide transport [Evidence IEA]"
                     /codon_start=2
                     /transl_table=11
                     /product="energy-coupling factor transporter ATPase"
                     /protein_id="HEX9917056.1"
                     /translation="KLSGGEKQRVALASVLAMQPKYLVLDEATSLLDSKDRKDFLALI
                     ERLSSQGITLIHITQFPEEANLAKRLVVLSEGKIVMDGSPLGIFQRVRELENIGLDAP
                     FNYRMSNLLARKGWLDQGRVYSIEEIADKIAQKIPNPDAEEKTKDETETIIEVENLSF
                     SYAQATPDHKKVLAGVSFKVDKGDLVGLIGPSGSGKSTLVQHLNRLLKPSGGKVIYKG
                     KDINSKNVNLKDIRQKVALTFQFPELQLFAETVFDDIAFGPRNLGLGEEEVRKRVKGS
                     MGILGLDFESFALRSPFSLSEGEKRRVAIAGVLSLNPEVLILDEPTVGLDPQNTRNLK
                     SIIKNLNQNGMTIILVSHNLNLIAEIAQKVILLDQGRIINYCSKQEFFEDLDRLGRLD
                     LEIPPTVYLISKLKEKGVSIIENSYDLEKLVQDA"
BASE COUNT         2208 a         1872 c         1413 g         2552 t
ORIGIN      
        1 ttcttattca atctcaatcg ttgtcatctg tttgacctaa aaagcgggga aaacactaag
       61 ggctgatcct attttctatc ctagccctac ccagttgtaa agaacccaaa attcaaaacc
      121 aaaccgaatc aaaccaaatc gagatcccga aacaagttcg ggatgacaaa aaccgtaaaa
      181 ccagatcagg ctgtcatgct gacggttcga ctacgctcac cgtccctgag caaagtcgaa
      241 gggaactcgt ttcagcatct ctggcctgtc gccaggacag tcttgtttca gcatctgctt
      301 gtttcagcat ctctggcctg acgccggaat aggctttagt tagtttgttc ttttctgcct
      361 ccaaaagata cacctttcat cgcccacggg aggggggttc aggtgcgatc ttgataaatc
      421 ctcttatctt ctcagctatc tggtctctaa cttttctaaa acctttcatt ttctcgtctt
      481 catcaccaga agagttggct gggtcttcaa agctccaata acttttgtta gctccgcccg
      541 gaaagtttgg acaagtttcc ctggcattct cacagacggt tattatgtag tcgaacttct
      601 ctttcaaaca aacttctacc gatttggagt tttgttttga gatatcaatc cctatctcct
      661 gcattaccct caccgccaat ggattaagga tggaggggga aataccggcg ctttgcgcat
      721 catatttatt gcctgccaaa cttctcaaga aaccttgagc catctgactt cgagatgaat
      781 tcgcggtgta gaggaaaagc actttccact ttcttcccaa agttttctcc ttttctgccg
      841 atttttaaaa ctaagagctt ctttgaaaaa tagcaaagat aaaataaagt ttagttgacg
      901 actgcaagaa tatattttat atttatggaa tcttaagggg aattgcaacc tatgccaaga
      961 aagatagttt tagcttatgt ctcagctgga gcaggacaca agagcgcctg catggctttg
     1021 caaaaggcat atctccagct tcatcccgaa gatcaagttc aggtagttga tatattgaag
     1081 tacgccccct ggccttttaa aacaatctac gctgagggat atcttttagt agctaacctt
     1141 caccctaccc tctggggatg gatctataac aaacaggtgg atatttataa aggcaaaaag
     1201 gagggcatat tctctacctg gctaaatcaa tttatcacca agagattcca aaaattcata
     1261 actaacattt ctccggatgt tgttatctcc gcgcatttct tatccagctg ggcagcagct
     1321 gaggcaagaa agaaaaacgc actcaacttc gtattgggca ctgtcgtaac tgactatgct
     1381 ctgcactccg cctggctggt gaaagaacaa gacctgttct ttgtctccac agaggaaatt
     1441 caatggcaac taattaagca taaggattat ttgggccttg ataaaaagga gattattgtc
     1501 tccggcatac ccatagaccc ggtttacgcc caaaagaaag ataaaaataa actgcgagaa
     1561 aaacgcaatc tgcaacccga cctttttacc attttgattc tgggtggaat ggctggctca
     1621 accaagataa acgacataat ctcttccatt ttgcgaattc atcttccttt tcagttgatg
     1681 atggtatctg gagctaactt ttgtattagt gccgcacacc aaaaagagat gaaaaaaaga
     1741 ggtattccct gtgtctgctt tggatatatt gattttatgg atgaggtgat gcagatggct
     1801 gatctggctt tgactaaagc cggcggactt gtttgcactg aatgtatgag taaaggtttg
     1861 ccggtagtca tcttcaggcc ttatcccggc caggaggaaa ggaataccga ctatttcctg
     1921 gagcagggtg cggctatgaa agttaatcag ttagatagct tggatttgaa gataattcag
     1981 cttatggaaa atccggaaaa gttggataag ttaagtgaga atgccttgca cattgtcaac
     2041 ccttctgctg ccctagatat tgtgaagcga attgatgaaa gagtacagtc aaaaagagcg
     2101 gcggcagtta atctttcatc actctaaatg cagttactgt gtttttctct ttatctgcca
     2161 acacgtcttt aaccttgatg gttaagatat aatccccggg tttaagctcc aacgcatcta
     2221 tctcgtctat ctcgtaagtg ttggatttcg tgcattcaac cggattggtc ttgtatagag
     2281 tagttctctt tcttgtcttg gtatttatta agtcggcttg tatgatgtaa taggcttttc
     2341 cgctcttatc caatttcaaa tcgtaaatct cataatagaa ataaatcttc tggtcagcaa
     2401 aatatatcct cgagggcaac gggcttatca ccaaaccatt acgttgcatt aacaaactat
     2461 catcttgtgt tgcctttcta acgtccgtgg ccatcactat ctgactgatt tctctttgtt
     2521 cgggatgaac ataaccgggc aataggatgt cctttttgta gattccaatt cggctggaaa
     2581 ctaaatccct tacctctact gcaactttat actctcctga attcagtaca aacctgtttt
     2641 ggtctatcac caatgaattg acagcttgac caactctcac attcacgatg gagctatctc
     2701 tctttacctc tttgtaatta ttgtcaaaaa caactatgct cttaacgaat gaaacataat
     2761 ttgcatcttt tttgcctaat tgctctatgg gcagaccaag gtttacatca acttcataat
     2821 tatcccccgt atttctgaat ctgactatgt ctaaagcata atccaaaacg tcgccttcat
     2881 aatcgtgatg gtaaatctcg cgaggaatgt caatcttggt ggctaccgtt gatttgaaat
     2941 caacttcatc ttgcacctca taggtttctc cataaacgtc ctttgattgc acttccacat
     3001 gaggagttag ctcataaaaa cccaaaagct tattatcctg aaactgcaat gtcaaatcat
     3061 acttataata tctccaaact tcaccttcct gttttcttgc ttcttgttct tcctttccaa
     3121 ccccatacac tccttccact tgtgtaaccc tctcatccgg ctcaccatat ttgatatata
     3181 cttctcctct gtcatcccaa aggcgattgc ctctgaaatg cgagccaaag tattgtctgg
     3241 cataagccac ccttctttga tgttcctctt tgaactcgtt tctctcggtg gtgggggtcg
     3301 ggtctttggc tttccagaat tttcttagcc attcctctct ttgtgtttca tctgtcatct
     3361 gtaaaaacga atctgcatcg tcttcggtca tcaccggttt tagttcgaga tattgtatcc
     3421 gatgttcagg caatgctcta tcccaagcct tgcgatatgc agtatctatg cttatgctaa
     3481 cgcaactgca gagcaataaa cctattgcga gcaacgaaat tgtcattcta tttaacacat
     3541 aacccccttt ctctaaatta acttctacga attacccctc ttatattgtt acaaattctg
     3601 tgccaataat ctgtcatcta gctcagattg tttgtaatca attgcacaat atgatgatga
     3661 agaaacttca actacgctgg ttttctgctc agaaaacacg agaatcgcaa gaagttgcga
     3721 ggttgttatt gaggatactc gcaagtcttt atcccaaaat aaaaaattac ccgcaagaaa
     3781 ttgcgggtta tcctccaaga atggaggctt aaactcaagc tggaatgatt ttggagtaga
     3841 cttatggttt cttttctgaa acttccgaaa tattatatac ctttatcttc cgcctcaagg
     3901 tcgattctgg catatccagc atcttagccg cactggcttt gatattatta caaaccttga
     3961 gcgctttccc tatctcctca atttccattt ccacttttct ttccgctatt gagccattct
     4021 ggtgaggtcc taatttctcc atcctcatct gctggatgaa cctatctaaa tcgctccctt
     4081 gctttgttcc cataagggca gagaatttca ggatttcatt ctctaattca cgaacgtttc
     4141 ctggccaatc atagaaaaac aaactctctg ctatctttcg gctcaatgtc tctattttat
     4201 cgcaattgcc attacaatgt ttctccaaga aataatggca taaaggcaaa acatcttctt
     4261 ttctttctct cagtggaggt aattccacct ttattacact caggcgatag tacagatctt
     4321 cccggaagcg cctttgttcc accgctttct tcaaatcctg gttggtggca gcaataaacc
     4381 taacattcac ttttatgggt ttagtgtctc ccaagcgggt gaattctttt tcttcgatta
     4441 cgttgagcag cttagcttga acggacaatg gaatctcccc gatttcattt aaaagcagtg
     4501 tgccgccatc cacctcttcc aaaagcccct tcttatccaa gttggcaccg gtgaaagcgc
     4561 ccttcttgta tccaaaaagt tcgctttcaa agagggtttc tggtaagtta gcacagttta
     4621 aggcaatgta ccttttgtct ttcctattgc cattataatg aatggtttta gctaaaaggt
     4681 cttttcctgt cccggtttca ccttctaaca gtatggttaa atcggtattc aaaacctgct
     4741 ccattctttt gattatatcc aaaagctccc gattttgggt aactatgttc gagaagatca
     4801 aatttgattt cttgtcagct atactttctt gaccagcttt actctcaagt ttctttctga
     4861 ttctaattgc caggctcaaa tcctctttct cgttcgattc cccaaatatc ttctctgctt
     4921 ctttggcaaa ggagattccc ttttcatatt gctcattttc atatagtagg tcagccaggg
     4981 ctaaataatt cttagctaaa agatatttcg aatcagtatc tcttaaggca tcctcagcct
     5041 gacccaacag tttcagtctt tcaaagtagc tataacaatc tgattttccg gcctcaagat
     5101 aaactttacc caactcgtac ttggcaccta tttctttgag aatagaaatt cccttctcaa
     5161 agttttcttt ggccaattca aattctcctt tgctggcata aacctgaccc aagattctat
     5221 aaactatccc ctcttcaaat ctctctttga tcttttggca aacacctaaa gaacgctgac
     5281 agaatgctga agcttcatcg taatttcctt gagcaaatcg caattcagcc aaaagccgac
     5341 aggtctggct tatgatatca ccttcaggag caatctcctc tcctattttc aaggcttcct
     5401 gataatgtct ttccgctgaa agaagatcgc cggtaataaa agtcagctgc cctgaatatt
     5461 cgtgataaat agctttctct ctcgttgcac cgtgccttaa tatcaagtca tagcttttat
     5521 ccaagtaatc ctcagcatca gcgagttttc ttctcatcat aaaaacgtaa gcgtaaccaa
     5581 gaaaccaatc aatctggaaa gcctcatttt gctgcatact gggatgcttg aaaagaagac
     5641 gaagttcttc tgctaattcg aattttccca agaaaacgta aactaaacct aagttgccac
     5701 ccaactggaa taacacattc ctatcattca aactctttgc gcattctatg ccgtctctca
     5761 gatactcaat cgcttcccca aatttcccct cgacaaaaca tacatgggct aatttattgt
     5821 ataaatgaag cgatttctta caaggctctt ggctacgata gaaggcaaag gagtccctgt
     5881 attctgcttg ggcatttttt aggtctccca accgaaggta ggtatcgccc aagattgtct
     5941 gaattctaac tattttttct atttcgtttg tatgtttcaa gatatcaaaa gccttcaagc
     6001 ccttttctaa ggcctcttga tgattaccca gattattgag agttgcggag aagatagtat
     6061 agaagtgacc ggattgggaa ttcgcttcaa taaaaacctt ctgattctcc aactctctga
     6121 gttcagctaa agccagcttg aatttcttct caaaaagaag caaatccagc cgctccaact
     6181 catatagcgg tttttccttt tcaggatcag atagtttttt catcctatct tccgtttacc
     6241 cttttgattt ctaacattag gaaatccagc cacacaatta ctctgttatt gttgccatcg
     6301 tttctccaac tattcccgtc aatagggaga cttgctctgc taagaggctc acccctattt
     6361 tcaatagaag gacgagtatg tggaatcccc acggccttat cttctatcca cgggcttgcc
     6421 ttaggccaag gacgtgctga gctgtttggg ataaaaacga agccggcaaa aaagatacaa
     6481 agcaacagcg tccaaatgat ttttcgaatc atttttgaat cgacctcctt aaaaagaata
     6541 agattatccc ctttaattag ggcatttgat tttcaattaa atagaactta aatggtaaga
     6601 aacaaaccta agcgagcctc cccctctttc atccctatcc cccagctgga tgaagtcagt
     6661 aaatccccac cttttcaata taacgagtag tcaaaatctg tcaagttctt tttggagaaa
     6721 caagaaaaca aaattgcaga acgttcttta ctctggcggt ttttcaggca tcttgcacca
     6781 acttttctaa atcataggaa ttctcgatta tactgacccc tttttcctta agctttgaga
     6841 ttaaatagac cgttggggga atctccaagt cgagcctacc caatctatct aaatcctcaa
     6901 agaattcctg tttggagcaa tagttaatta ttctgccctg atccaaaagt atcacctttt
     6961 gtgctatttc ggctattaga ttcaggttgt gagaaacaag gattatggtc attccgtttt
     7021 gatttagatt cttgattatt gatttcaaat ttctggtatt ttgtgggtcc aaaccaactg
     7081 tcggctcatc caaaattaag acttcaggat tcaaagagag aactcctgct atggcaactc
     7141 ttcttttttc tccctcgctt agggaaaaag gtgaacgaag ggcgaaactc tcaaaatcca
     7201 atcctaaaat acccattgag cctttcactc tttttctcac ttcttcttcg cccaacccca
     7261 aatttctcgg accgaaagcg atatcatcga aaaccgtttc cgcaaaaagc tgaagctccg
     7321 ggaattgaaa agttaaagca actttctggc gaatatcttt cagattaacg ttttttgagt
     7381 ttatgtcttt acctttataa attactttac ctcccgatgg cttcaaaagc ctatttagat
     7441 gttgaaccag agtgctctta cctgagccac tcggaccgat taaaccaacc aaatcaccct
     7501 tgtcgacttt aaagcttact ccagctagaa ctttcttatg gtcgggagtt gcctgtgcat
     7561 aagaaaagga aaggttttca acttcgatta tggtttcagt ctcgtctttt gttttctctt
     7621 ccgcatccgg atttggaatc ttctgagcta ttttatctgc aatctcttcg atcgaataca
     7681 ctcttccctg gtctaaccag cccttgcgcg ctaaaagatt tgacattctg taattaaagg
     7741 gggcatctaa acctatattc tctaactctc ttaccctttg aaagatgcct aaaggagagc
     7801 catccataac tatcttccct tcagataaaa ccactaacct ttttgctaaa ttcgcctctt
     7861 cgggaaattg agtgatatgg atcaaggtta tgccttgaga tgataatctt tctatcagag
     7921 ccaaaaaatc ttttctgtct tttgaatcca gaagcgaagt cgcttcatcc agaaccagat
     7981 atttcggctg cattgccaga accgaagcta aagctaccct ctgcttttct ccaccggaaa
     8041 gctta
//
LOCUS       DASWLN010000146         3070 bp    DNA     linear   ENV 20-FEB-2024
DEFINITION  MAG TPA_asm: Candidatus Zixiibacteriota bacterium isolate
            SMAG_U2949 k141_754054, whole genome shotgun sequence.
ACCESSION   DASWLN010000146 DASWLN010000000
VERSION     DASWLN010000146.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37960594
            Sequence Read Archive: SRR10376091
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Candidatus Zixiibacteriota bacterium (soil metagenome)
  ORGANISM  Candidatus Zixiibacteriota bacterium
            Bacteria.
REFERENCE   1  (bases 1 to 3070)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 3070)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (26-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/12/2024 08:28:26
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 1,440
            CDSs (total)                      :: 1,416
            Genes (coding)                    :: 1,412
            CDSs (with protein)               :: 1,412
            Genes (RNA)                       :: 24
            rRNAs                             :: 1 (16S)
            partial rRNAs                     :: 1 (16S)
            tRNAs                             :: 21
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 4
            CDSs (without protein)            :: 4
            Pseudo Genes (ambiguous residues) :: 0 of 4
            Pseudo Genes (frameshifted)       :: 0 of 4
            Pseudo Genes (incomplete)         :: 3 of 4
            Pseudo Genes (internal stop)      :: 1 of 4
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..3070
                     /organism="Candidatus Zixiibacteriota bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_754054"
                     /isolate="SMAG_U2949"
                     /isolation_source="soil"
                     /db_xref="taxon:2053527"
                     /environmental_sample
                     /country="USA: Oregon"
                     /lat_lon="42.99 N 123.63 W"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            complement(446..976)
                     /locus_tag="VGB16_04905"
     CDS             complement(446..976)
                     /locus_tag="VGB16_04905"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_012415641.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="2-oxoacid:acceptor oxidoreductase family
                     protein"
                     /protein_id="HEX9917057.1"
                     /translation="MYQGVIMSGFGGQGIISAGVILAYAGMIDGKHVTFFPAYGAEMR
                     GGTANCSVIVSDQEIAAPVVSTPESLIVMNEPSLARFETTLKPKGLLLINSSLVSGKP
                     KRGDIDVHYIPANGVAEEIGSIRCANMVMLGALAKRTGAVKIESIMKCLPKVFTKAKK
                     EILDMNIEAMRRGAQS"
     gene            complement(1007..1786)
                     /locus_tag="VGB16_04910"
     CDS             complement(1007..1786)
                     /locus_tag="VGB16_04910"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_012448118.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003824 - catalytic activity [Evidence
                     IEA];
                     GO_function: GO:0030976 - thiamine pyrophosphate binding
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="thiamine pyrophosphate-dependent enzyme"
                     /protein_id="HEX9917058.1"
                     /translation="MEKKVFSRNEGLTDIKMRYCPGCGHGIVHRMIGDVLVELGIRKR
                     TIGIAPVGCSVFADEYFNCDMIQPAHGRGPAVATGMKRVKPDCIVFSYQGDGDLASIG
                     MAETIHSAARGENITIIFINNAIFGMTGGQMAPTTLIGQKSTTSPAGRDVKLAGYPIR
                     VCELLSALNGPAYLVRTSLHNSANVVKTKKAIKRAFQIQMEGKGFSLVEILSQCPTNW
                     GMTTLKSIEYIAENMVPYYPLGEFRTPEDKKAKAEAETLKG"
     gene            complement(1802..2857)
                     /gene="vorB"
                     /locus_tag="VGB16_04915"
     CDS             complement(1802..2857)
                     /gene="vorB"
                     /locus_tag="VGB16_04915"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_012635623.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="3-methyl-2-oxobutanoate dehydrogenase subunit
                     VorB"
                     /protein_id="HEX9917059.1"
                     /translation="MSERILMKGNEALAEGAVRAGCRFFAGYPITPQNEVPEYMSWRM
                     AQVGGTFIQAESEVAAINMLFGAAACGARVMTSSSSPGISLKQEGISYTASAELPIFY
                     ANVTRGGPGLGNIAPSQGDYYQATRGGGHGDYRVIVLAPNSVNNMGNFPKMCYDLAFK
                     YRIPSMVLADGVLGQMMEPLEFDFEPIDPKKLPEGEWALGNAEGRERKVVRSYDLKPG
                     GMEKHNWKLDKKYKEIEEKEVLYQTFSVDDAEILIVAFGTCSRIAKSALSLGRSQKLK
                     IGLLRPITLWPFPTKILNQLASKIKKILVVEMNLGQMLDDVKIAVEGKAEIGFHGRPA
                     GGIPTPEEILEKVKEMV"
     gene            complement(2860..3066)
                     /locus_tag="VGB16_04920"
     CDS             complement(2860..3066)
                     /locus_tag="VGB16_04920"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003542506.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="4Fe-4S dicluster domain-containing protein"
                     /protein_id="HEX9917060.1"
                     /translation="MTRCKGCELCRVACPHNVIEMSKKFSPTGYYPAIMVRPEKCTGC
                     ALCAMVCPDVSIEVYKEETVSKEK"
BASE COUNT          761 a          815 c          578 g          916 t
ORIGIN      
        1 tttcgtaaag gtccggaaac cccaaagcta ctttcgtctt gccggggtgc tcctttctaa
       61 tcacatttaa ttcattcccg acgtagcgtc ctggcttgac gacaaaggga agaaattgct
      121 tttcaatctt ttctgctaac ggaaccatta ttgtttagta aaaataataa ttagtatgtt
      181 taaaagttaa acactttctt gccttttgtc tcctctcccc tgcggggaga ggattaaggt
      241 gagggggctt cttaagagag tttgctcccc ctcaccctat ccctctcccc aaaggggaga
      301 gggaatttcc ttctttaatt agggaaaagt ataattttgc caagctctat attataatta
      361 ttatcttgag aaatgtggtc aagactaaaa agtcgccaaa ctttcggcga ctttcaatta
      421 aaagtatatt tcgctttctg tcaagctaag attgggctcc tcttctcatg gcttcaatat
      481 tcatatccag aatctctttc ttggctttgg tgaatacctt gggcaggcac ttcataatgc
      541 tttcgatttt gaccgcacca gtcctttttg ccaatgcacc cagcatcacc atattggcac
      601 atcttatgct tccaatctcc tcggctacgc cgttagcggg tatataatgc acgtcgatgt
      661 cgcctctttt aggtttgcct gaaactaagg agctatttat caaaagcaat cccttgggct
      721 tgagtgttgt ctcgaatcga gccaatgacg gctcgttcat cactattaag ctttcgggcg
      781 tggacaccac tggagcggct atttcctggt cggagacgat gactgagcaa ttggcggttc
      841 cacctctcat ctctgcccca taagcaggaa aaaaagtaac atgtttgcca tctatcattc
      901 cagcgtaagc taagattacc ccagccgaga ttatcccctg tcctccgaaa cctgacatta
      961 tcacaccttg atacattgtc cctccagata tcgacaaatt aagtttttaa ccttttagag
     1021 tttccgcttc cgccttggct ttcttatcct ccggagtcct gaattctcct aagggataat
     1081 aaggaaccat attctcggca atgtactcaa tcgacttaag cgtcgtcata ccccaattgg
     1141 tcggacattg agatagaatc tcaaccaagg aaaaaccctt tccttccatc tgaatctgaa
     1201 aagctctttt tatcgccttc ttggtcttga ccacatttgc ggagttatgc aaggatgttc
     1261 taactaaata agctggacca ttcaaagcgg acaaaagctc acacactctg attggatacc
     1321 cagccagctt cacatctctt cccgccgggc tggtagtgga tttctgtcct atcaaagtgg
     1381 tgggagccat ctgaccaccg gtcattccaa aaatggcgtt gttgatgaaa attatggtta
     1441 tgttttcgcc tcgagccgcg ctatgaattg tctccgccat gccaattgaa gccaagtccc
     1501 catctccctg atagctgaaa acgatgcaat ccggcttaac tctcttcatt ccggttgcca
     1561 ctgctggtcc cctgccatgg gctggctgaa tcatatcgca gttgaaatac tcatccgcga
     1621 aaactgagca ccctactgga gctattccga tggttctctt tcgtattccc agctcaacta
     1681 atacatctcc aatcattcta tgaactatgc catgaccgca tcccggacag tagcgcattt
     1741 tgatatcagt taaaccttca tttctactaa ataccttctt ttccattttt cctcctgggg
     1801 ctcaaaccat ctctttaacc ttctcgagaa tctcttccgg agttggtatc ccgccagctg
     1861 gacggccatg aaagccgatt tcagcctttc cctctactgc tatcttaaca tcatctaaca
     1921 tctgccctaa gttcatctcc accactaata tcttctttat cttagaagcc agttgattca
     1981 atattttggt tgggaagggc cataaagtta taggtctgag aagacctatt ttgagtttct
     2041 gcgagcgacc taaactcagg gctgatttgg caatgcggga acaggtgcca aaggcaacta
     2101 tcaaaatctc cgcatcatca actgagaagg tttggtataa aacctctttt tcttctatct
     2161 ctttgtattt cttgtccaat ttccagttat gcttttccat tcccccgggc ttcaaatcgt
     2221 atgaccttac tacctttctt tccctgcctt ccgcatttcc caaagcccat tccccttccg
     2281 gcaacttctt ggggtcaatt ggctcgaaat caaattccaa cggctccatc atctgaccca
     2341 atactccgtc tgccaaaacc atactcggaa tccgatattt aaatgccaaa tcataacaca
     2401 ttttagggaa attccccata ttattgaccg aattcggagc taagactata actctataat
     2461 caccgtgccc accccctctg gttgcctgat aataatcacc ttgagaaggc gcaatgtttc
     2521 ccaatcccgg acctcctcga gttacgttgg cataaaaaat tggaagttca gcgctggcag
     2581 tgtatgagat tccttcctgc ttgaggctta ttcccggact agaggaggaa gtcatgactc
     2641 ttgctccaca ggcagctgcg ccaaagagca tatttatggc ggccacttcg ctttccgcct
     2701 gaatgaaagt tcctcccact tgagccattc tccaggacat atactctggg acttcgttct
     2761 ggggagttat gggatagccg gcaaaaaaac gacatcctgc cctgactgct ccctcggcca
     2821 atgcttcgtt acctttcatt aagattctct cactcatctt catttctcct ttgaaaccgt
     2881 ctcttcttta tagacctcaa ttgacacatc cggacaaacc atagcacaca aggcacaacc
     2941 ggtgcatttt tccggtctta ccatgatcgc aggataatat ccggtggggc tgaacttctt
     3001 ggacatctcg ataacgttat gcgggcatgc caccctgcac agttcgcacc ctttgcagcg
     3061 tgttatatca
//
LOCUS       DASWLN010000147         3662 bp    DNA     linear   ENV 20-FEB-2024
DEFINITION  MAG TPA_asm: Candidatus Zixiibacteriota bacterium isolate
            SMAG_U2949 k141_629365, whole genome shotgun sequence.
ACCESSION   DASWLN010000147 DASWLN010000000
VERSION     DASWLN010000147.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37960594
            Sequence Read Archive: SRR10376091
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Candidatus Zixiibacteriota bacterium (soil metagenome)
  ORGANISM  Candidatus Zixiibacteriota bacterium
            Bacteria.
REFERENCE   1  (bases 1 to 3662)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 3662)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (26-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/12/2024 08:28:26
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 1,440
            CDSs (total)                      :: 1,416
            Genes (coding)                    :: 1,412
            CDSs (with protein)               :: 1,412
            Genes (RNA)                       :: 24
            rRNAs                             :: 1 (16S)
            partial rRNAs                     :: 1 (16S)
            tRNAs                             :: 21
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 4
            CDSs (without protein)            :: 4
            Pseudo Genes (ambiguous residues) :: 0 of 4
            Pseudo Genes (frameshifted)       :: 0 of 4
            Pseudo Genes (incomplete)         :: 3 of 4
            Pseudo Genes (internal stop)      :: 1 of 4
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..3662
                     /organism="Candidatus Zixiibacteriota bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_629365"
                     /isolate="SMAG_U2949"
                     /isolation_source="soil"
                     /db_xref="taxon:2053527"
                     /environmental_sample
                     /country="USA: Oregon"
                     /lat_lon="42.99 N 123.63 W"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            13..1377
                     /gene="pheT"
                     /locus_tag="VGB16_04925"
     CDS             13..1377
                     /gene="pheT"
                     /locus_tag="VGB16_04925"
                     /EC_number="6.1.1.20"
                     /inference="COORDINATES: protein motif:HMM:TIGR00472.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0009328 - phenylalanine-tRNA ligase
                     complex [Evidence IEA];
                     GO_function: GO:0004826 - phenylalanine-tRNA ligase
                     activity [Evidence IEA];
                     GO_process: GO:0006432 - phenylalanyl-tRNA aminoacylation
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="phenylalanine--tRNA ligase subunit beta"
                     /protein_id="HEX9917061.1"
                     /translation="MIRRGRMKLGISTESSYRFERGADPNITELAIDRAAQLMSQLAG
                     GEVLQGRVDNYAKPISPLKITLRPKRANQLLDISLSSQEMNGILESLELKTQDRGEIL
                     EVEIPTFRPDLTRGIDLIEEIARVYGYDKIGLSSRSGGSLLNQISTEEKIQKRVKNLL
                     IGRGFFEIITSNLVDPKLLEKINPQEKPLTIRNPLSEELSVLATSLACGMLAVIAHNK
                     NHKQKDLKLFELGKVILPKAKEKLPEERHDLCLALSGRRKPMQWGENYKEEVDFFDLK
                     GIIEEILQDMNVSNLKFVAHTREPFERECSLTLSIDNEVIGFCGQVARAILDDFGIKD
                     KVFLAELNFDLLSKWSCFEKSFTPLLRFPSSRRDLAIIVDQEILSGDIEESIRAGGKD
                     LVEELVLFDLYQGKQVPKGKKSLAYSISYRSSQKTLTDEEVEKAHQNIVSRLKKQFGA
                     ELRK"
     gene            1393..1677
                     /locus_tag="VGB16_04930"
     CDS             1393..1677
                     /locus_tag="VGB16_04930"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEX9917062.1"
                     /translation="MQLETFDKLEKKINLVLKAMDRLKEENKQLSSSYSELRVGIGGM
                     KKTSGELEEENRKLKEKLSRIEQTRTQREMRIKAKLEKLVERLGALEGLS"
     gene            1939..2238
                     /locus_tag="VGB16_04935"
     CDS             1939..2238
                     /locus_tag="VGB16_04935"
                     /inference="COORDINATES: protein motif:HMM:NF017016.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_process: GO:0000917 - division septum assembly
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="cell division protein ZapA"
                     /protein_id="HEX9917063.1"
                     /translation="MENKKQSVTVNIFGEDYPIRSDVDNEYTRKIATYLDRKMKEVAE
                     GLPSRTYSKVAVLAAMNIADELFKEKIDKENKLSQVEEKAQTLLKWLDDSLREEA"
     gene            2349..>3662
                     /gene="rny"
                     /locus_tag="VGB16_04940"
     CDS             2349..>3662
                     /gene="rny"
                     /locus_tag="VGB16_04940"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_008909074.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004521 - RNA endonuclease activity
                     [Evidence IEA];
                     GO_process: GO:0006402 - mRNA catabolic process [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="ribonuclease Y"
                     /protein_id="HEX9917064.1"
                     /translation="MIPVWMIISGGVLVAVLAFILGWFVSRRMGESKLAKLEELAGKI
                     IQEAEKEAEIKKKEAVLEAKDEWYKAKVNFEREIQNKKMELEKLEKRFQEREVGLDRK
                     VDILNKKERDLQNKDKTIFAREKAIRLRDAELDRLVAEQNKQLERVAQMTAEEAKKIL
                     MENLEAQAQREAAQLIKDIKEKAEQTAEKEAKNIIISAIQRCAADHAMETTVSVVNLP
                     NDEMKGRIIGREGRNIRSFETATGIDVIVDDTPEAVILSGYDPIRREIARMALEKLIA
                     DGRIHPARIEEVVEKSEKEMEVIIRETGEQACFDVGIHGVHPELVKLLGKLRYRTSYG
                     QNVLQHSEEVAFLSGLMAAELEMDSNLAKRCGLLHDIGKAVDRETEGTHSEIGYNIAQ
                     KYGEHPVALNAIASHHEDVVQESAYSVLVQAADAISGARPGARRET"
BASE COUNT         1191 a          634 c          969 g          868 t
ORIGIN      
        1 tttgacccga gggtgataag gaggggccga atgaagctcg gaatttcaac tgaatcctcc
       61 taccgcttcg aaagaggtgc tgacccgaat attaccgagc ttgctattga ccgcgcagcg
      121 caactaatga gccaattagc cggaggagaa gtcctgcaag gcagagtgga taattatgct
      181 aagccgatct ctcccctgaa aatcacgctg aggcctaaaa gggcaaacca gcttttggac
      241 atctcccttt cttctcaaga gatgaatggt atacttgaaa gcctggaatt gaaaactcaa
      301 gataggggtg agattctcga agtggagata ccgactttta gacctgacct gacaagggga
      361 atcgacctga ttgaggaaat tgccagagtc tatggctacg ataagatagg actatcatcc
      421 agaagcggtg gctctttatt gaatcagatt tcaactgagg aaaagataca gaaaagggtt
      481 aagaatttat taattggaag agggtttttt gaaataatca ccagcaactt agttgaccct
      541 aagcttctag aaaaaataaa tccccaggaa aagcccttga caatacgaaa tcctctaagt
      601 gaagagcttt ctgttcttgc cacttctctg gcttgcggca tgttggcagt tattgcccat
      661 aacaagaatc ataaacaaaa agacctgaaa ttgtttgaat tgggcaaagt catccttcct
      721 aaagcaaaag aaaaactgcc ggaggaaagg catgacctat gtttagctct ttctggaaga
      781 cgtaagccga tgcaatgggg agagaactat aaagaggaag tagatttctt tgaccttaaa
      841 gggataatag aggaaatact ccaagatatg aacgtgtcta atttgaagtt tgttgctcac
      901 accagagagc cctttgaaag agaatgctcc ttaacccttt caatcgataa cgaagtgatt
      961 ggattctgcg gacaggtggc gagggcgatt ctggatgatt ttggtattaa agataaggtt
     1021 tttttggcgg aattgaattt tgaccttttg agcaaatggt cgtgttttga aaagagtttc
     1081 acacctttat tgagatttcc cagttccaga cgggacctgg ccataatagt tgaccaggaa
     1141 atactctctg gagatattga agaaagcata agagcaggcg gtaaggattt agtggaggag
     1201 ctggtgttgt ttgaccttta ccaaggcaag caagttccga agggcaagaa gagcttggct
     1261 tattccatct cctaccggtc ttcacaaaag actttaacgg atgaagaagt ggagaaagcg
     1321 catcaaaata tagttagtcg attgaaaaag caatttggag cggaattgcg caaataggaa
     1381 aaggaaaagc gtatgcaatt ggaaactttc gacaaactgg aaaaaaagat aaatttagtt
     1441 ttaaaagcaa tggaccgctt aaaagaggag aataagcagc tttcttcctc ttacagcgaa
     1501 ttgagggttg gaataggagg gatgaagaaa acaagcggcg agcttgaaga ggaaaaccga
     1561 aaattgaagg agaagctctc gagaattgag caaaccagga cccagaggga aatgaggata
     1621 aaagccaagc tggaaaagct ggtggagagg cttggtgcat tggaaggatt gagttagttg
     1681 agaggttgaa aaagttgaac agggaaaggt ctttctggag tgtcccgctt tagcggggcc
     1741 taaaaacctt ttgtccacag gatcccgctc tacgggacaa acaaagaacc ctaatgcggt
     1801 tcacctaaag gtgaacactc caaattaccc agtggtctct aattaatgct tgacaatgtg
     1861 tttttctaat cgtatttttt aattaaataa aagggtgata ggtaaggttt taaccatttc
     1921 gttttcaaat cagatattat ggaaaataag aagcaatctg tgacggtaaa tatttttggt
     1981 gaagattatc cgatcagaag cgatgttgac aatgaataca caaggaaaat tgcaacttac
     2041 cttgatcgga agatgaagga agtggcggaa ggactgccaa gccggactta tagtaaagtg
     2101 gctgtcttgg ctgccatgaa tatcgcagat gagctcttta aagagaagat agataaagag
     2161 aataaacttt ctcaagttga agagaaggcc caaactctct tgaaatggtt agatgacagt
     2221 ttaagggaag aagcttaact tatctatccc cacttgcagt ttagcagatt caattagatt
     2281 tatcagagtc taattgaaga cgacactatg gtgagtgggg ttttttgttt tagcataagg
     2341 aggaagttat gattccggtt tggatgataa tctcaggtgg tgtactggta gctgtgctgg
     2401 ctttcatttt gggttggttt gttagccgta gaatggggga gagcaaatta gccaagttgg
     2461 aggagttagc cggcaagata atacaggaag cggaaaagga agctgaaatc aagaaaaaag
     2521 aggcggtctt agaagccaag gatgaatggt acaaagccaa agtgaacttt gagcgcgaaa
     2581 tccagaacaa gaagatggaa ctggaaaagc ttgagaagag attccaggaa agggaagtgg
     2641 gcttagaccg caaggtagat attttaaaca agaaagaaag agatttacag aataaggata
     2701 aaactatatt tgccagagaa aaagctattc ggttgcgtga tgccgagctg gaccgacttg
     2761 tggctgagca gaataaacag ctggagaggg ttgctcagat gaccgcagag gaggcgaaga
     2821 aaattctgat ggagaacctg gaagcacagg cacaaaggga agcggctcag ctaatcaagg
     2881 acataaaaga gaaggcggag caaacagcag agaaagaagc aaagaatatc atcatctcgg
     2941 ctattcaacg ttgcgcggct gaccatgcta tggagactac tgtgtcagtg gtcaatctgc
     3001 cgaatgacga gatgaaaggg agaataatcg gcagggaagg cagaaatatt aggtcttttg
     3061 agaccgccac ggggattgat gttatcgtag acgacacccc agaggcagtt atcctttccg
     3121 gttacgaccc cataagaaga gaaatagccc gtatggctct cgagaagttg attgccgatg
     3181 gcaggattca cccagccaga attgaagagg tggttgagaa atccgagaag gagatggaag
     3241 tgataatccg cgaaaccgga gagcaggctt gctttgatgt gggtatacat ggagtccatc
     3301 cggaactagt aaaacttttg ggcaagctgc gatatagaac cagctatgga cagaatgtac
     3361 tccagcacag tgaagaggtg gcattcttat ccgggctgat ggcagccgaa ctggagatgg
     3421 actcgaattt agccaagagg tgcggcttgt tacatgacat cggcaaggcg gttgaccgag
     3481 aaactgaagg gactcattct gaaataggtt ataacatagc tcaaaaatac ggagagcatc
     3541 cggtagcatt aaacgctatt gcctcacatc atgaagatgt ggtgcaggag tcagcttatt
     3601 cagtcttggt tcaggcggcg gatgcgattt ccggagccag acccggtgcc agaagagaaa
     3661 cc
//
LOCUS       DASWLN010000148         3603 bp    DNA     linear   ENV 20-FEB-2024
DEFINITION  MAG TPA_asm: Candidatus Zixiibacteriota bacterium isolate
            SMAG_U2949 k141_373199, whole genome shotgun sequence.
ACCESSION   DASWLN010000148 DASWLN010000000
VERSION     DASWLN010000148.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37960594
            Sequence Read Archive: SRR10376091
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Candidatus Zixiibacteriota bacterium (soil metagenome)
  ORGANISM  Candidatus Zixiibacteriota bacterium
            Bacteria.
REFERENCE   1  (bases 1 to 3603)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 3603)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (26-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/12/2024 08:28:26
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 1,440
            CDSs (total)                      :: 1,416
            Genes (coding)                    :: 1,412
            CDSs (with protein)               :: 1,412
            Genes (RNA)                       :: 24
            rRNAs                             :: 1 (16S)
            partial rRNAs                     :: 1 (16S)
            tRNAs                             :: 21
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 4
            CDSs (without protein)            :: 4
            Pseudo Genes (ambiguous residues) :: 0 of 4
            Pseudo Genes (frameshifted)       :: 0 of 4
            Pseudo Genes (incomplete)         :: 3 of 4
            Pseudo Genes (internal stop)      :: 1 of 4
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..3603
                     /organism="Candidatus Zixiibacteriota bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_373199"
                     /isolate="SMAG_U2949"
                     /isolation_source="soil"
                     /db_xref="taxon:2053527"
                     /environmental_sample
                     /country="USA: Oregon"
                     /lat_lon="42.99 N 123.63 W"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            complement(478..3360)
                     /locus_tag="VGB16_04945"
     CDS             complement(478..3360)
                     /locus_tag="VGB16_04945"
                     /inference="COORDINATES: protein motif:HMM:NF012385.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_function: GO:0008134 - transcription factor binding
                     [Evidence IEA];
                     GO_process: GO:0006355 - regulation of DNA-templated
                     transcription [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="sigma 54-interacting transcriptional regulator"
                     /protein_id="HEX9917065.1"
                     /translation="MKREKLTERLKYIERLIEKGDYALAEKNIDILQGKDISDLSLSQ
                     QGELHYLSALLFSKLARYQEGLEQANIAFELLKDTCENEKIAKVNLLAGKLYTNLGDL
                     KHAELELRDALSAYRRIGDQENRIEVYNRLARICFTKGEYDRAIGYLDEALELSLSLN
                     NSRFTAMLFGNLGRIYLRLGEFNQAQERLLSSSDIYEELKDYENMGRRYLSLGYLYFS
                     QRKFKQAEEFYQKAKTIIDQKNLTYELANFHEYFAEYCLEIGNYTKAQKHIQEAIDLG
                     QKIAPDSSIMTQSYRVLAQILLAQKKYDQALEACDKSLGVPLEERLEQGIVHRIVGQI
                     YAAQNKRDLSKESFVKSIAILDEIKAKFELAKTYLEAGKSAVFDYSQSLEYLQKSKLL
                     FGQVDDQYNLALTDINLAKLRFDKGDCDQAITSLNNAVAILEKMDEKSLLSQAEKIRS
                     EIENQISQESLSANNEYRIFRKYLPENEYKGIEDVKIDSSLEILAKKIKADRGFVTSV
                     DANREMSIASTFNLDPTQATQVVSCLKNLNSGYSYLKSPAIYTKADSHPKLFSLFPEG
                     ENKISSLISIPLKSIEGEITGFLYLDRKSNGKGNPPFSSQEFNFAVAFGDLLAIKLTH
                     LEKKKLEEDNINLRRQLEEKCCFSNIITTDQKMLNALKKTEQLKDSNLPILIEGETGT
                     GKDLLAKAIHYHSNRKDKKFVAINCAAFPDTLLESELFGHKKGAYTGAVEDKKGLLEE
                     ADGGTLYIDEVADMPLTTQVKLLRALEEKEIIRLGETVPRKVDIRVISATNKKIEEEM
                     AAGRFRKDLYYRLNTVNVKLIPLRERKGDIPLLTEHFIKIYSKDPSEKELSRIIPTMI
                     ELFMGHDWPGNVRELESEVRKLLALRDESGRICSDLLSDRFCLPEKTNLKELSLYQRV
                     GIWEKQYILKALLENNWIKKATASVLNIPESSLRFKMKQYNIVKPQ"
     gene            complement(3376..>3603)
                     /locus_tag="VGB16_04950"
     CDS             complement(3376..>3603)
                     /locus_tag="VGB16_04950"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEX9917066.1"
                     /translation="AGYKRARGLPSDDPRTNVVDPWEGTEKENNGPTTSGIKSAIGQN
                     NSCSLIFIDKVGNIPVVFKVEVKNHLSKGKK"
BASE COUNT          924 a          785 c          723 g         1171 t
ORIGIN      
        1 atactcaggc cttcggctct gaggctcagg ctcgaagaga tggtaagtgg ttcgacttcc
       61 ttcgacttcg ctcaggacaa atcgctcacc accctgagga attcgaaggg caaggtcgaa
      121 ccataaaggc gtgggaatct aaaaagatag gagccttgtc cgtttgccct ctattatacc
      181 gagacaaggc tccagggggt ttagtgtgga agaaagggaa aaaccgctaa ttagtttcat
      241 atcaaaatct atgccaagtt aattcccctt ttttcttttt tgaaaagttt tttaacttat
      301 tttgaaacaa taagttagaa gaatacataa gagagctaat aaatcgagtt ttattccaaa
      361 aacgcaacaa gttgcgaata gaaaagccaa aaatttcttg taatatgatt aaaccattgt
      421 tttgtaaggg attaaactaa cgcaagattt ggcggtaaat agataggagt tttgctgtta
      481 ttgcggtttg actatgttat actgtttcat cttgaaacgc aaagatgatt ccgggatatt
      541 taaaactgag gcagttgctt tcttaatcca attgttttca agcaaagcct tcaagatata
      601 ttgcttctcc cagatgccca ccctctggta cagagaaagc tcttttagat ttgttttctc
      661 cggcaggcag aatctatcag agagaagatc cgagcatatc cttccacttt catctcttag
      721 agcaagcaac ttcctgacct cactttccag ttctcttacg tttccgggcc aatcgtggcc
      781 cataaatagc tcaatcattg tgggaataat ccgggaaagc tccttttcgg aggggtcctt
      841 agaataaatc tttatgaaat gttcagtcaa aaggggtata tccccttttc tttccctcag
      901 gggaatgagt ttcacattca ccgtattgag gcggtaatag aggtctttgc ggaaacggcc
      961 cgctgccatt tcctcttcaa ttttcttgtt ggtggcggag atgacccgga tatcaacttt
     1021 tctgggaaca gtctcgccca aacggattat ctctttctcc tctaaagcac gcagaagttt
     1081 aacctgagta gtaagaggca tatccgccac ctcatctata taaagtgtgc ctccgtctgc
     1141 ctcttctaag agccccttct tgtcttcaac cgcgccagta taagcacctt tcttatggcc
     1201 aaaaagctca ctttccaaaa gggtatccgg aaaggcggca cagttgattg ccacaaattt
     1261 cttgtcttta cgattgctgt gatagtgaat ggctttagcc aaaaggtctt tgccggtgcc
     1321 ggtttcaccc tcaattagta taggtaaatt ggaatcctta agctgctctg ttttcttcaa
     1381 ggcatttaac atcttctggt cagtggttat gatgttagaa aagcagcatt tctcttccaa
     1441 ttgtcttctt aggtttatat tatcttcttc caattttttc ttttccagat gagtcaactt
     1501 gattgccaat aaatcaccaa aagcaactgc gaaattgaat tcctgcgaac tgaagggcgg
     1561 attgcctttg ccgttagatt ttctatccaa atacagaaat ccggttatct ccccttcgat
     1621 gctctttaag ggtatggaga ttaagctgga aattttattc tctccctccg ggaaaagact
     1681 aaataacttg gggtgggagt ccgccttagt atatatggcc gggctcttaa gataggaata
     1741 gccgctattt aagttcttaa ggcaggatac cacttgagtg gcttgtgttg gatccaagtt
     1801 gaaggttgag gcgatggaca tttcccggtt ggcgtcaact gaagtgacaa accctctatc
     1861 tgcctttatc tttttggcaa gtatttccaa ggagctatct attttaacat cttcaattcc
     1921 tttatattcg ttttccggca gatactttct gaagatgcga tattcattat tagctgatag
     1981 actctcctgg ctgatttggt tttctatttc gcttcttatt ttttctgcct gagatagtag
     2041 gctcttttca tccatctttt ccagaattgc taccgcattg ttgagggagg ttatggcttg
     2101 gtcgcaatct cccttgtcga atctcagctt agccagatta atatcagtta atgcaagatt
     2161 gtattggtca tccacctgcc caaagaggag cttggacttc tggagatatt caaggctctg
     2221 gctataatcg aagaccgctg attttcctgc ctcgaggtag gttttggcta attcgaattt
     2281 ggccttaatt tcatctaata tggctataga cttaacgaaa ctctcttttg agaggtctcg
     2341 cttgttttga gctgcataaa tctgaccgac tattctatgt actatcccct gctccagcct
     2401 ttcctcaaga ggaactccaa gcgatttatc gcaggcttcc agagcttggt cgtatttctt
     2461 ttgagctaag aggatttgtg ccagcaccct gtagctctgg gtcattatgc tggagtccgg
     2521 ggctattttt tggcctaaat cgattgcttc ttgaatatgt ttctgggctt ttgtatagtt
     2581 gccaatttcc agacaatatt cagcgaaata ttcatggaag ttggccaact cgtaggtgag
     2641 atttttttgg tctattatag ttttggcttt ttggtaaaac tcttctgctt gcttgaactt
     2701 tctttgggag aagtatagat agcccaagga gagatatctt cgtcccatgt tttcgtagtc
     2761 ctttagctct tcatatatat ccgaagacga caagagcctt tcctgggctt ggttgaattc
     2821 gcctaaacgc agatagatcc tacctaaatt accaaagagc attgcagtaa acctagaatt
     2881 attcaagctc aaagaaagct ctaaggcttc atcgagatac cctatggctc tgtcgtattc
     2941 tcccttggtg aaacatatac gggctaaccg attatacact tcgattctat tttcctggtc
     3001 tccaattcta cgataagcag atagggcatc tcgcaattca agctccgcat gctttagatc
     3061 tcccaaattg gtgtaaagtt tgccagcaag aaggttaact ttagctatct tctcattttc
     3121 acaagtatcc ttgagcaatt caaaggcaat attagcttgc tctaatccct cttggtagcg
     3181 agccagctta gagaaaagca aagcgctcaa gtaatgcagt tcaccttgct gggataagct
     3241 caaatcggat atatccttgc cctgcaagat atctatgttt ttctcagcta aagcataatc
     3301 tcctttttct atcagtcttt ctatatactt tagcctctct gtaagttttt cccttttcat
     3361 tcccttatct accttttatt tttttccctt agagagatgg tttttcactt ccaccttgaa
     3421 aaccacaggg atattgccga ccttatcaat gaaaatcaag ctgcaagagt tattctgacc
     3481 aatggcactc ttgataccgc tagtggtagg accattgttc tccttttctg ttccttccca
     3541 aggatccacg acatttgttc gagggtcatc agaaggaagc cctcgagcgc gcttataacc
     3601 agc
//
LOCUS       DASWLN010000149         4367 bp    DNA     linear   ENV 20-FEB-2024
DEFINITION  MAG TPA_asm: Candidatus Zixiibacteriota bacterium isolate
            SMAG_U2949 k141_824095, whole genome shotgun sequence.
ACCESSION   DASWLN010000149 DASWLN010000000
VERSION     DASWLN010000149.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37960594
            Sequence Read Archive: SRR10376091
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Candidatus Zixiibacteriota bacterium (soil metagenome)
  ORGANISM  Candidatus Zixiibacteriota bacterium
            Bacteria.
REFERENCE   1  (bases 1 to 4367)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 4367)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (26-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/12/2024 08:28:26
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 1,440
            CDSs (total)                      :: 1,416
            Genes (coding)                    :: 1,412
            CDSs (with protein)               :: 1,412
            Genes (RNA)                       :: 24
            rRNAs                             :: 1 (16S)
            partial rRNAs                     :: 1 (16S)
            tRNAs                             :: 21
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 4
            CDSs (without protein)            :: 4
            Pseudo Genes (ambiguous residues) :: 0 of 4
            Pseudo Genes (frameshifted)       :: 0 of 4
            Pseudo Genes (incomplete)         :: 3 of 4
            Pseudo Genes (internal stop)      :: 1 of 4
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..4367
                     /organism="Candidatus Zixiibacteriota bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_824095"
                     /isolate="SMAG_U2949"
                     /isolation_source="soil"
                     /db_xref="taxon:2053527"
                     /environmental_sample
                     /country="USA: Oregon"
                     /lat_lon="42.99 N 123.63 W"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            <1..628
                     /gene="fliG"
                     /locus_tag="VGB16_04955"
     CDS             <1..628
                     /gene="fliG"
                     /locus_tag="VGB16_04955"
                     /inference="COORDINATES: protein motif:HMM:TIGR00207.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0009433 - bacterial-type flagellum basal
                     body, C ring [Evidence IEA];
                     GO_function: GO:0005198 - structural molecule activity
                     [Evidence IEA];
                     GO_process: GO:0001539 - cilium or flagellum-dependent
                     cell motility [Evidence IEA];
                     GO_process: GO:0006935 - chemotaxis [Evidence IEA]"
                     /codon_start=2
                     /transl_table=11
                     /product="flagellar motor switch protein FliG"
                     /protein_id="HEX9917067.1"
                     /translation="QTISLVLTQLPAQQAAAVLSELSPELQAEVALRIATMEKISPEV
                     VSEIETVLESQLETTETKGLSTLGGTKTVAEILNLVDLSAEKDILQALETENPELAAE
                     IKNLMFVFEDIILLDDRAIQRVLKEIETRELTIALKATSEEVRKKILSNVSERVAAMI
                     KEEVEFMGPMRLTDVEASQQRIVEAIRRLEEEGQIVVAGRGGKEEIVV"
     gene            621..1271
                     /locus_tag="VGB16_04960"
     CDS             621..1271
                     /locus_tag="VGB16_04960"
                     /inference="COORDINATES: protein motif:HMM:NF014196.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="FliH/SctL family protein"
                     /protein_id="HEX9917068.1"
                     /translation="MSKIIRPNVLLKEKVPIGERDSEDEYLETGGASPDSTRPATLAQ
                     KIQREREKAYRSGFEDGKQMGLEEGREQGEKIAKEFFSLLEDIKIQRANIFKEAEQAV
                     VKLALALASKIICGAVETKPEVVLDVTRNAIHHLVDKNKVVLRVNSKDYELVKAHHND
                     LLASIDGIKSLEIEEDIRVKLGGCLIETDSGNVDARLENQMQVLKEALVGKAQGSL"
     gene            1401..2804
                     /gene="fliI"
                     /locus_tag="VGB16_04965"
     CDS             1401..2804
                     /gene="fliI"
                     /locus_tag="VGB16_04965"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_006694583.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0009288 - bacterial-type flagellum
                     [Evidence IEA];
                     GO_function: GO:0016887 - ATP hydrolysis activity
                     [Evidence IEA];
                     GO_process: GO:0001539 - cilium or flagellum-dependent
                     cell motility [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="flagellar protein export ATPase FliI"
                     /protein_id="HEX9917069.1"
                     /translation="MQVDYQKYSDILERTDPVKRDGKVCQVIGLVIESTGPAVSVGEM
                     CYIESQDKNLKIKSEVVGFRDKKVMLMPLGNMDGVSPGSTVIASGEPFFVSVGHELLG
                     RVIDGLGEPIDSKEQIITKHRKPIYNSPPSPLARRRIKEPLYTGIKAIDSLVTFGKGQ
                     RAGIFSGSGVGKSIVLGMIARESAADVNVIGLVGERGREVREFIEKDLGEDGLKRSVV
                     VAVTSDKPALIRLRGALIATTIAEYFRDLGMDVMLMMDSLTRVAMAQREIGLAIGEPP
                     TTKGYTPSVFALLPKLLERAGASDVGSITGLYTVLVEGDDLSEPVSDAARAILDGHIV
                     LSRKLAALNHYPAVDVLGSVSRVMIDVISDEHRKLADKAKEVLANYRESEDLINIGAY
                     VKGSNPKVDYAISKIEALNNFLKQGIGDKADFKNNIETLKEIFKDEGIQVSAAEGDGV
                     PPAPRKREAARLSSSKK"
     gene            2743..3162
                     /gene="fliJ"
                     /locus_tag="VGB16_04970"
     CDS             2743..3162
                     /gene="fliJ"
                     /locus_tag="VGB16_04970"
                     /inference="COORDINATES: protein motif:HMM:TIGR02473.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0009288 - bacterial-type flagellum
                     [Evidence IEA];
                     GO_component: GO:0016020 - membrane [Evidence IEA];
                     GO_function: GO:0003774 - cytoskeletal motor activity
                     [Evidence IEA];
                     GO_process: GO:0006935 - chemotaxis [Evidence IEA];
                     GO_process: GO:0071973 - bacterial-type
                     flagellum-dependent cell motility [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="flagellar export protein FliJ"
                     /protein_id="HEX9917070.1"
                     /translation="MEFRQHLEKEKQRDLAQAKNKLQQEEKKLELLNHTKDECQKETR
                     QKKKKDKINLKELSLYQIYLTKLHHQIKGQRIIVVRTEEEVEGRREELLEKSREKKVL
                     ENLRKGRLFSYKEEANRIEQKEIDELARDICLRKKKR"
     gene            3374..4033
                     /locus_tag="VGB16_04975"
     CDS             3374..4033
                     /locus_tag="VGB16_04975"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEX9917071.1"
                     /translation="MVEEKIEKEKTRTGMKKETKPVAPPKKSKLVLFVGAGLVVFIVF
                     LVFFSFSLGVFDKERLKGELGLTEDTTASITQAQSEDSAAETYVDNSESTAQPEEDTA
                     AVASEDSVKSWIDEQKAKIQEDRTVVEVQRRELELLKIEVGNLLKQKKAFEDERIGKL
                     AKIYDGMKAQEVAAMMNNLDDDTVVAVLLKMSTRNAAKVLGLLNAERAARISNKLMAL
                     T"
BASE COUNT         1367 a          746 c         1207 g         1047 t
ORIGIN      
        1 tcagaccatt tccttagttt tgacccagct tccggctcaa caagcagctg cagtgctttc
       61 tgaactatcg cctgagcttc aagctgaggt ggctttacgc attgccacta tggagaagat
      121 atcgccggag gttgtgagtg aaatagaaac cgtgctggaa tcacagttag agaccacgga
      181 aaccaaggga ttatccactt taggcggcac taagaccgtt gccgaaatac taaacctggt
      241 ggacctttca gcagagaagg atatcctgca agcgttggaa actgaaaatc cggagttggc
      301 cgcagaaata aaaaatctga tgttcgtgtt cgaggatata atccttctgg atgatagggc
      361 gattcagaga gtgctaaaag agatagagac cagagaattg actatcgcct tgaaggccac
      421 ttccgaagag gtgagaaaga aaatattatc taacgtctca gagagggtgg cagcgatgat
      481 taaagaagag gtggagttta tgggacctat gcgtctgact gacgtggaag cgtctcaaca
      541 gaggattgtg gaagctataa gacgcctgga agaagaaggg cagattgtcg ttgctggaag
      601 aggaggcaag gaagaaattg ttgtctaaga tcattcgacc taatgtgctg cttaaggaaa
      661 aagtgccaat tggagagaga gattcggagg atgaatatct cgaaacaggg ggagcttctc
      721 cagatagcac caggcctgcc accttagccc aaaaaattca aagagaaagg gagaaagcat
      781 atcgctccgg ctttgaggat gggaagcaga tgggattgga agagggcaga gagcaagggg
      841 agaagatagc caaggaattc ttctctctgt tagaagatat taagattcag agagcaaata
      901 tcttcaagga ggcggagcaa gcggtggtca agcttgccct ggctttagct tccaagataa
      961 tatgcggtgc ggtggaaaca aaaccagaag tggtcttgga cgtaaccaga aacgcaatcc
     1021 atcatttagt ggataagaat aaggtagtcc ttcgagtaaa ttctaaagat tatgaactgg
     1081 tcaaagcaca tcacaacgat cttttggcga gcatagatgg cataaaaagt ctggagattg
     1141 aagaagatat cagggtgaag ctgggtggat gcttgattga gaccgattca ggaaatgtgg
     1201 atgcccgcct ggaaaatcag atgcaagtcc tgaaggaagc tttggtgggc aaagctcaag
     1261 gaagtttgtg aattgaaatc tcgaagcttc acaaaaagat ataatgattc tcagttttta
     1321 gagtctctat cccgataaat cgggacgatt tttgaatttg gctgacaaaa aagatattta
     1381 tacccaaaca gaagattaag atgcaggtag attatcagaa atattcggat attttggaga
     1441 ggaccgaccc ggtgaaaagg gatgggaagg tctgtcaggt gattggcttg gtcattgaat
     1501 caactggacc agcagtttca gtgggcgaga tgtgttatat cgaaagtcag gataaaaatc
     1561 taaagataaa atcggaagtg gttggattta gagataagaa agttatgctt atgcctctgg
     1621 gaaatatgga tggggttagt ccgggaagca cagtcattgc ttcgggcgag ccgttttttg
     1681 tcagcgtcgg gcatgagctt ttggggaggg ttatagatgg tctgggtgaa cccatagata
     1741 gcaaggagca gataatcact aaacatagaa aaccgattta caattccccg cctagtccat
     1801 tagcccgaag aagaataaag gagccgcttt ataccggcat taaggcaatc gatagcctgg
     1861 tgacctttgg caagggacaa agggcgggca tattttcggg aagtggtgtg gggaaaagca
     1921 tagttttggg aatgatcgct cgtgagtcgg ccgcggatgt gaatgtcatc ggtctggtag
     1981 gggaacgggg aagagaggtg agggagttca tagaaaagga tttgggcgaa gatggtttga
     2041 aaaggtcggt agtggttgct gtgacctcag ataagccagc tttgattaga ttacgaggag
     2101 cgttgattgc aactaccata gctgaatatt tccgcgattt aggaatggat gtgatgctga
     2161 tgatggattc gctgacgcgt gtggctatgg cccaaaggga aatcggctta gccattggag
     2221 agcccccgac caccaaaggt tatacccctt cggtttttgc tttgttgcct aaacttttag
     2281 agagggctgg agcctcggat gtgggaagta tcaccgggct ttatacggtc ttagttgaag
     2341 gagacgattt aagtgagccg gtgtcagatg cggcacgggc tattctagat ggacatatcg
     2401 tcttgtctcg gaaattggcg gcattaaatc attatcccgc agtggatgtc ttaggatcag
     2461 tgagcagagt gatgatagat gtcatctcgg atgaacatcg caaattagct gataaagcca
     2521 aggaggtctt agccaattat cgcgagtctg aagacctgat aaatattgga gcatatgtca
     2581 aaggttcaaa tcctaaagtg gattatgcca tttccaagat tgaggccttg aacaattttc
     2641 ttaagcaggg gataggggat aaggcggatt tcaagaataa tattgagaca ttgaaggaga
     2701 tattcaaaga tgaaggtatt caagtttcgg ctgcagaagg tgatggagtt ccgccagcac
     2761 ctagaaaaag agaagcagcg agacttagct caagcaaaaa ataaactcca acaggaagag
     2821 aaaaaactcg agcttctcaa tcacaccaaa gatgagtgcc agaaggagac caggcagaag
     2881 aagaagaagg ataaaatcaa tcttaaggag ctttcccttt atcagatata tctgactaag
     2941 ttgcaccatc aaatcaaggg acagaggata atcgtggtca ggactgaaga ggaggtcgag
     3001 gggagaaggg aagagcttct ggaaaagtca agagagaaga aggtgctgga gaatttaaga
     3061 aaaggaagac tgttttccta taaagaagaa gcaaatcgga ttgagcagaa ggaaatagac
     3121 gagcttgccc gcgatatatg cctcaggaag aagaaaaggt aactttccaa ggaaaaattt
     3181 accgctttgg tttggaaata ttttctaaag attccaaccg acttacaatt tcagataatc
     3241 atcttaccag agtaagttcc tctaattaaa gggattatcc atacttaatc agtccggcag
     3301 gtaagttggc acagctgttg cttacacgaa atttagggtt tccatgttct tgtccttatt
     3361 aggaggttaa aaagtggtag aggaaaagat agaaaaggaa aaaaccagaa caggaatgaa
     3421 aaaggaaact aagccagtgg caccacccaa gaagagtaag ctcgtgcttt tcgtgggtgc
     3481 ggggttggtt gtctttatag tatttctggt tttcttttca ttttctttgg gcgtgttcga
     3541 caaggaaaga ctaaaaggag aattgggact gacagaggac acaactgctt ccattactca
     3601 ggcgcaatca gaagatagcg cagcagaaac ttatgttgac aatagcgagt caactgcaca
     3661 gccagaagaa gataccgctg cagtagctag cgaggattcg gtcaaaagct ggatagacga
     3721 gcagaaagca aagattcagg aggaccgtac tgtagtggag gtgcagcgtc gagagttgga
     3781 gcttctgaaa atcgaggttg gtaatttgct taaacagaag aaagcttttg aggatgagag
     3841 gataggtaaa ctggctaaaa tatatgacgg gatgaaagct caggaggtgg cggcaatgat
     3901 gaataatctg gatgacgata ctgtggtagc agtattgtta aagatgagca ccagaaatgc
     3961 tgccaaggtc ttggggcttt tgaatgcgga gagagcggcc cggatcagta acaaattaat
     4021 ggctctaaca tgatggaagg aactatgttc agcaacttct tggtaccgaa ctcagttagt
     4081 cttttcccgt ttgctcctcg taaacttgaa gggggaatta ctcaaaaggg cccccaaggg
     4141 gaaagttctg aatttaagca gttgattgaa tgcttaagga ggtccccaga actgggacag
     4201 aatcagaacc tattgggttc tttagggttc ccccctttca agtcaaacga gtttccattg
     4261 accgaacaac tttcggaaat gctccttggt agtcagctgg atgcggtatc cgtagagcaa
     4321 tcaactgaaa aggcagttta tgtaaatgaa aaagggttgt gccatct
//
LOCUS       DASWLN010000150         7311 bp    DNA     linear   ENV 20-FEB-2024
DEFINITION  MAG TPA_asm: Candidatus Zixiibacteriota bacterium isolate
            SMAG_U2949 k141_1207665, whole genome shotgun sequence.
ACCESSION   DASWLN010000150 DASWLN010000000
VERSION     DASWLN010000150.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37960594
            Sequence Read Archive: SRR10376091
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Candidatus Zixiibacteriota bacterium (soil metagenome)
  ORGANISM  Candidatus Zixiibacteriota bacterium
            Bacteria.
REFERENCE   1  (bases 1 to 7311)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 7311)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (26-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/12/2024 08:28:26
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 1,440
            CDSs (total)                      :: 1,416
            Genes (coding)                    :: 1,412
            CDSs (with protein)               :: 1,412
            Genes (RNA)                       :: 24
            rRNAs                             :: 1 (16S)
            partial rRNAs                     :: 1 (16S)
            tRNAs                             :: 21
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 4
            CDSs (without protein)            :: 4
            Pseudo Genes (ambiguous residues) :: 0 of 4
            Pseudo Genes (frameshifted)       :: 0 of 4
            Pseudo Genes (incomplete)         :: 3 of 4
            Pseudo Genes (internal stop)      :: 1 of 4
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..7311
                     /organism="Candidatus Zixiibacteriota bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_1207665"
                     /isolate="SMAG_U2949"
                     /isolation_source="soil"
                     /db_xref="taxon:2053527"
                     /environmental_sample
                     /country="USA: Oregon"
                     /lat_lon="42.99 N 123.63 W"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            complement(<1..115)
                     /locus_tag="VGB16_04980"
     CDS             complement(<1..115)
                     /locus_tag="VGB16_04980"
                     /EC_number="1.1.1.267"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011142249.1"
                     /note="catalyzes the NADP-dependent rearrangement and
                     reduction of 1-deoxy-D-xylulose-5-phosphate (DXP) to
                     2-C-methyl-D-erythritol 4-phosphate; Derived by automated
                     computational analysis using gene prediction method:
                     Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="1-deoxy-D-xylulose-5-phosphate reductoisomerase"
                     /protein_id="HEX9917072.1"
                     /translation="MKQIALLGSTGSIGTSTLEVIRRFPKEFEVFGISAHRN"
     gene            complement(716..1345)
                     /locus_tag="VGB16_04985"
     CDS             complement(716..1345)
                     /locus_tag="VGB16_04985"
                     /EC_number="5.2.1.8"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_019879741.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003755 - peptidyl-prolyl cis-trans
                     isomerase activity [Evidence IEA];
                     GO_function: GO:0016018 - cyclosporin A binding [Evidence
                     IEA];
                     GO_process: GO:0000413 - protein peptidyl-prolyl
                     isomerization [Evidence IEA];
                     GO_process: GO:0006457 - protein folding [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="peptidylprolyl isomerase"
                     /protein_id="HEX9917073.1"
                     /translation="MKKVLVLFLVLCFAFVSCGKKEVKKEEAVKVALDKANMADTIQK
                     IPDFVSEKNPMVVIETDFGNMEMEIFLKETPKTAQNFLKLVSAGFYDSLIFHRVIPDF
                     VIQGGDPLGDGTGGPGYYINDEIGMKHKLGSVGMATSGPNTAGSQFYICLKDLPQLDK
                     NYTVFGKIISGMEVAQKIADVETTVDPNSGEKSIPVQKVYMKKVYIKQT"
     gene            complement(1620..2300)
                     /locus_tag="VGB16_04990"
     CDS             complement(1620..2300)
                     /locus_tag="VGB16_04990"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEX9917074.1"
                     /translation="MPKGEHSKSLGMAMHYNEEDSRLVNGSVAHESFNPNNATIDFCY
                     RKGMGRDLDIGVRWGSGGLGIDVKRSMINSQKPWIPRLALDLDFLVNNGIFIGQDFEG
                     GVAVSCITDRKVRRNLGLINSLSLKYYMMNFPPNTQLSEEGKSMVRDMTGSVSNVVEK
                     SIILELPVGLQFFGKNENRSFLVGVIPQVYLYRKTKEITIETDYFLDILEYSHKYDVQ
                     FILQYSFF"
     gene            complement(2430..2843)
                     /gene="atpC"
                     /locus_tag="VGB16_04995"
     CDS             complement(2430..2843)
                     /gene="atpC"
                     /locus_tag="VGB16_04995"
                     /EC_number="7.1.2.2"
                     /inference="COORDINATES: protein motif:HMM:TIGR01216.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0000275 - mitochondrial
                     proton-transporting ATP synthase complex, catalytic sector
                     F(1) [Evidence IEA];
                     GO_component: GO:0045261 - proton-transporting ATP
                     synthase complex, catalytic core F(1) [Evidence IEA];
                     GO_component: GO:0045262 - plasma membrane
                     proton-transporting ATP synthase complex, catalytic core
                     F(1) [Evidence IEA];
                     GO_function: GO:0046933 - proton-transporting ATP synthase
                     activity, rotational mechanism [Evidence IEA];
                     GO_process: GO:0015986 - proton motive force-driven ATP
                     synthesis [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="ATP synthase F1 subunit epsilon"
                     /protein_id="HEX9917075.1"
                     /translation="MFDLSVVTPEKIFYEDKINSLVIPATLGYLGVLTNHAPLLTGLV
                     PGRITIKDQQGKRRILAVSGGFTEVLKNRVTVLADAVEFPDKIDLERAQKALERSKQR
                     LKSREKGIDILRATAAYRRAKNRIDIYKALSSASE"
     gene            complement(2846..4258)
                     /gene="atpD"
                     /locus_tag="VGB16_05000"
     CDS             complement(2846..4258)
                     /gene="atpD"
                     /locus_tag="VGB16_05000"
                     /EC_number="7.1.2.2"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_007958875.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0000275 - mitochondrial
                     proton-transporting ATP synthase complex, catalytic sector
                     F(1) [Evidence IEA];
                     GO_component: GO:0045261 - proton-transporting ATP
                     synthase complex, catalytic core F(1) [Evidence IEA];
                     GO_component: GO:0045262 - plasma membrane
                     proton-transporting ATP synthase complex, catalytic core
                     F(1) [Evidence IEA];
                     GO_function: GO:0046933 - proton-transporting ATP synthase
                     activity, rotational mechanism [Evidence IEA];
                     GO_process: GO:0015986 - proton motive force-driven ATP
                     synthesis [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="F0F1 ATP synthase subunit beta"
                     /protein_id="HEX9917076.1"
                     /translation="MNYGKVKQVIGPTVDIEFDTDKLPNILNAVKIEDKERGINLTVE
                     VALHVGDNLVRCISLASTDGLVRGMSALDTGGPISVPVGPQTLGRVFNLLGNPIDELG
                     PLKGEVKRYPIHRPPPSFEDQETKTSLFETGIKVIDLLEPFPRGGKIGLFGGAGVGKT
                     VMVIELIRNIATEHGGYSVFSGVGERTREGNDLWLQMRDSGVINKTVLVFGQMNEPPG
                     ARLRVGLSGVTMAEYFRDEEHQDVLLFIDNIFRFVQAGSEVSALLGRMPSAVGYQPTL
                     GTEMGALQERITSTKAGSITSVQAIYVPADDLTDPAPATTFSHLDAITVLSRQIVELG
                     IYPAVDPLGSTSRIMDPKVLGEEHYQTARAVQKILQRYKDLQDIIAILGTEELSEEDK
                     IIVARARKMQRFLSQPFFVAESFTGTKGKYVKLEDTIKGFKQIVEGKLDHLPEQAFYM
                     VGGIEEVFQKAERLKKGATR"
     gene            complement(4271..5125)
                     /gene="atpG"
                     /locus_tag="VGB16_05005"
     CDS             complement(4271..5125)
                     /gene="atpG"
                     /locus_tag="VGB16_05005"
                     /EC_number="7.1.2.2"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_013176426.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0000275 - mitochondrial
                     proton-transporting ATP synthase complex, catalytic sector
                     F(1) [Evidence IEA];
                     GO_component: GO:0045261 - proton-transporting ATP
                     synthase complex, catalytic core F(1) [Evidence IEA];
                     GO_component: GO:0045262 - plasma membrane
                     proton-transporting ATP synthase complex, catalytic core
                     F(1) [Evidence IEA];
                     GO_function: GO:0046933 - proton-transporting ATP synthase
                     activity, rotational mechanism [Evidence IEA];
                     GO_process: GO:0015986 - proton motive force-driven ATP
                     synthesis [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="ATP synthase F1 subunit gamma"
                     /protein_id="HEX9917077.1"
                     /translation="METLGDIRKRIRSVESTHEITKAMEMVAAVKLRRAQSKAESSRP
                     YALKMQEMLVNLSSASEEITHPLFEKREAKIVALVVITSDRGLCGSYNSNIIRAAQGF
                     IKNYTPQNVMIINVGKKGYDFYRKRGFEIKLKYLSLGGNLVTSNIKSISEDLTKLFLF
                     GEADEIHILYTKFISAMSYRVNLQKFLNIESKDKDEKEMVDYIFEPEPEEIFTELLPR
                     YCSTVIQMALAEAFASEHGSRMIAMGGATKNAEEMIEHLTLQRNKARQASITKELLEV
                     VSGAEALR"
     gene            complement(5127..6650)
                     /gene="atpA"
                     /locus_tag="VGB16_05010"
     CDS             complement(5127..6650)
                     /gene="atpA"
                     /locus_tag="VGB16_05010"
                     /EC_number="7.1.2.2"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011393857.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0000275 - mitochondrial
                     proton-transporting ATP synthase complex, catalytic sector
                     F(1) [Evidence IEA];
                     GO_component: GO:0045261 - proton-transporting ATP
                     synthase complex, catalytic core F(1) [Evidence IEA];
                     GO_component: GO:0045262 - plasma membrane
                     proton-transporting ATP synthase complex, catalytic core
                     F(1) [Evidence IEA];
                     GO_function: GO:0046933 - proton-transporting ATP synthase
                     activity, rotational mechanism [Evidence IEA];
                     GO_process: GO:0015986 - proton motive force-driven ATP
                     synthesis [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="F0F1 ATP synthase subunit alpha"
                     /protein_id="HEX9917078.1"
                     /translation="MSLKPEEVSLIIQKEIEKYQTKLQMESVGVVLQVGDGIARIWGL
                     EDAMMSELIKFPNDITGMILNLEEDNVGVVIFGSDTNIKEGDAVKRTGKIASVPVGEV
                     MLGRVVNALGEPIDDKGPIVTKKFRPIEGGAPNVVQRQPVKEPVQTGLKAIDSMIPIG
                     RGQRELVIGDRQTGKTALVLDTIVNQKNSDIYCIYVAIGQKASTVAQVVKTLEDYGAM
                     EYTTVVVAGATDPAPLQYIAPYAGCAMGEEFRDNKKHVLVIYDDLSKHAKAYRQLALL
                     LRRPPGREAYPGDIFYLHSRLLERAAKLSDELGGGSLTAIPIIETQAGDITSYISTNV
                     ISITDGQIFLESDLFYAGIRPAINVGISVSRVGGSAQTKSMKKVAGMLRLDLAQYREM
                     AAFAQFGTELDEATRAQITRGERTVEILKQEQYVPMLLEKQVMIIYTAVNGYLDDLKV
                     EVIRKFEEEFYPFMDKEYPDVVHAIAQTKDLDETTQKKLDEAIKKFKGQFVAQLKEK"
     gene            complement(6665..7213)
                     /gene="atpH"
                     /locus_tag="VGB16_05015"
     CDS             complement(6665..7213)
                     /gene="atpH"
                     /locus_tag="VGB16_05015"
                     /EC_number="7.1.2.2"
                     /inference="COORDINATES: protein motif:HMM:NF012439.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0000275 - mitochondrial
                     proton-transporting ATP synthase complex, catalytic sector
                     F(1) [Evidence IEA];
                     GO_component: GO:0045261 - proton-transporting ATP
                     synthase complex, catalytic core F(1) [Evidence IEA];
                     GO_component: GO:0045262 - plasma membrane
                     proton-transporting ATP synthase complex, catalytic core
                     F(1) [Evidence IEA];
                     GO_function: GO:0046933 - proton-transporting ATP synthase
                     activity, rotational mechanism [Evidence IEA];
                     GO_process: GO:0015986 - proton motive force-driven ATP
                     synthesis [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="ATP synthase F1 subunit delta"
                     /protein_id="HEX9917079.1"
                     /translation="MINPEIAKRYALGLFLAALKKDALERVLGEQKTVAALLREHTDF
                     KSFLEVPQITKKEKEEFLRRAFSGKLDAVFYHFLLVLLNRHRFEYLLPIADEYEKLVK
                     EHKGIIQVMVTTARPLSKDIAKKLQDRLEKKTGKEIEMLLKVNPAIIGGMALILENRI
                     IDHSISYYLRQLKEEMSGLRVS"
BASE COUNT         1902 a         1859 c         1312 g         2238 t
ORIGIN      
        1 tattgcggtg agctgatatt ccgaacacct caaactcttt cggaaaacgt cttatcactt
       61 ccagggtgct tgtcccgatt gagccggtcg aacctaataa tgctatttgt ttcatattaa
      121 tttgttcctt gaaaaataag acaagttgaa atataaatcc gatgccaaac gtaagcaagc
      181 aatttattaa aattacacga aatcgtaggc gacattccgg cctgcccggc cagggaatgt
      241 cgcctacaaa atagcgaggc agggtgcctc gcctacgaat ttgtgtttgt caggtccccc
      301 tgaagctaac cgagaattgt cattctgagc ccgtaagggc gaagaatctc gcttcccaac
      361 cttcataaaa ccaagattct tcactccgcg gggtttagcc tgaggcaacc cagacccttc
      421 gcttcgctca gggtgacaag tggtgaaggg ctccgttcag acctgtcctg aagcgaagtc
      481 gaaggaatga cacaattgga gttctcggtc acctttccct gtcttgacaa aaaaatctgc
      541 caagtcacat cctgcccgtt cttccctgct cgacccaacg gccagggggg acttggcagg
      601 tacggaaaaa atcaagacgg gatgaattcc tatccccgca ataacaaatt agtagggaca
      661 gaacatcgtt ctgtccctac actttaacaa atctctaatt ttatgtcaac cgctgctatg
      721 tctgcttgat atagaccttc ttcatataaa ctttctgaac cggtatgctc ttttcaccag
      781 aattaggatc aaccgtggtt tccacatcgg ctatcttttg cgccacttcc atcccgctta
      841 tgatttttcc aaacacggta taattcttgt ctagttgagg caggtctttc agacaaatat
      901 aaaactggct gccagctgtg tttggacccg aggtagccat cccaaccgaa ccaagtttat
      961 gtttcattcc aatttcatca ttgatataat aacctggtcc accagttcca tcacccaaag
     1021 gatctccacc ctgtatcaca aaatctggta ttacacgatg aaaaatcaga ctgtcataga
     1081 aaccggcact aactaatttc aggaaattct gagcagtttt cggtgtctct tttaagaaaa
     1141 tctccatttc catatttccg aaatcggtct cgattaccac catcgggttc ttctcactta
     1201 caaaatcagg gatcttctga atagtgtcag ccatattggc cttatctaaa gcgactttca
     1261 ctgcctcctc cttttttacc tcttttttgc cacaagaaac aaaggcaaaa caaaggacaa
     1321 gaaataacac taaaaccttc ttcataaaac ctcccttaaa tgaaggttaa tagattccca
     1381 gtttttacag gcatagttct tgttacttgt aggggcaatt cataaattgc tacaattata
     1441 ttctgtcatt cccgaaggtt taatcgggaa tccattctta cttttctctt ctggatttcc
     1501 cgtatccagt acgggacaag gtctgctgga gtttatcccg cagaaatgcg ggacaggaat
     1561 gacacaaaac ggaaaactcc tttcatcccc gccttaagag tcggggagtg cctgctggat
     1621 taaaaaaagc tatactgaag aataaattgc acatcgtatt tatgagaata ttctaaaatg
     1681 tcaagaaagt agtcagtctc tatagttatc tcctttgtct ttctatacaa ataaacttgc
     1741 ggtataacgc ctacaaggaa agatctgttt tcatttttac cgaaaaactg caaaccgacc
     1801 ggtaattcta aaattataga tttttcaacg acattcgaca cggagccggt catatctcta
     1861 accatactct taccctcttc agataattga gtgttcggag ggaaattcat catgtagtat
     1921 tttaaagaaa gggagttaat gagccctaaa tttctacgaa ctttccggtc tgtaatgcac
     1981 gaaacggcta cgccaccctc aaaatcttgc ccgataaaaa taccattgtt taccaaaaaa
     2041 tccaaatcaa gagctaatcg tggaatccac ggcttttgcg agtttatcat gctgcgcttc
     2101 acatctattc ccaatccacc actaccccac ctaacgccaa tatctaaatc gcgacccatt
     2161 ccctttctat agcaaaaatc tattgttgca ttgttagggt taaacgactc atgcgcaacg
     2221 gaaccattca ctaaacgact atcttcctca ttataatgca tcgccattcc caaagatttg
     2281 gaatgttctc cttttggcaa gacgcctgct gtctgtatat aagtcgttct agcgcaacta
     2341 aagagaagca ctatcaatag aagtagaaag aatttgcaaa gccgagagcg attatcttgc
     2401 attgccttca ttagttacat caattgtcgt tattctgaag ctgaagataa tgccttataa
     2461 atgtctatcc tatttttggc tcttctgtaa gctgccgtcg ctcgaagaat gtcaattcct
     2521 ttttctcttg atttcaatct ttgcttggat ctttccagag ctttttgggc tctctcgagg
     2581 tcaatcttat cagggaattc aactgcatct gccaaaacgg tcactcgatt tttcaagact
     2641 tcggtgaagc cgccgcttac tgccagaatt cgcctcttgc cctgctggtc tttgatagtt
     2701 atcctgccgg ggaccaggcc ggttaaaagt ggagcatgat tggtcagcac tcccagatat
     2761 cctaaagttg cgggaataac aagcgaattt atcttatcct cgtaaaatat tttctctgga
     2821 gtaacgacac ttaaatcaaa catacctatc ttgttgcacc tttctttaat ctttcagctt
     2881 tctggaacac ttcttctatt ccacccacca tatagaatgc ctgctcagga agatggtcta
     2941 atttaccttc cactatttgt ttgaatcctt tgatggtatc ttccaatttg acatactttc
     3001 ccttagtccc ggtgaaactt tctgccacga agaagggctg ggaaagaaac ctttgcatct
     3061 ttctggctcg ggcaactatt atcttatctt cctcagaaag ctcttccgtt cccaaaatgg
     3121 caattatgtc ctgcaggtct ttgtaccttt gcagaatctt ttgcactgcc ctggcagtct
     3181 ggtaatgctc ctcccctagg acctttgggt ccataatgcg cgaagttgaa cccaatgggt
     3241 caactgccgg ataaattccc aattcaacta tttgacgaga aagcacagtt attgcatcta
     3301 aatgggaaaa ggtcgtagca ggagcaggat cagttaaatc atctgccgga acatagatgg
     3361 cttgaaccga ggttatggag ccggccttgg ttgaagttat cctttcctgt aaagctccca
     3421 tttcggtgcc taaagttggt tgatagccaa ctgccgaggg cattctgccc aatagagcgg
     3481 agacttctga accagcttgc acaaatctaa aaatgttatc gataaacaaa agaacatctt
     3541 gatgctcttc atccctaaag tactccgcca tagtcacacc agataagcct actctcaatc
     3601 ttgctcctgg tggctcattc atctgtccga aaaccagaac cgtcttgttt atcacgccag
     3661 aatccctcat ctgtaaccat aggtcatttc cttctctggt cctctccccc acaccggaaa
     3721 aaaccgaata ccctccatgc tcggtggcaa tgtttcttat tagctctatc accataaccg
     3781 ttttccccac tcctgcacca ccaaacagcc ctatcttacc tcctctggga aaaggctcca
     3841 gaaggtcaat aacttttatt ccggtttcaa aaagcgaggt ctttgtttct tgatcctcaa
     3901 agctgggcgg gggtctatga atggggtatc tttttacttc tcccttaagc gggccaagct
     3961 catcaatcgg atttcccaga agattgaata ctcttcccaa ggtttgtgga ccaaccggaa
     4021 cacttatcgg tcctccggta tccaaagccg acatccccct gaccaaaccg tcagttgaag
     4081 ccagtgatat gcacctcacc aggttgtccc caacgtgcaa ggcaacttcg actgtcagat
     4141 ttattcccct ctccttatct tcaattttta ctgcattcaa tatgttggga agtttatccg
     4201 tatcgaattc gatgtcaact gtgggtccaa tgacctgttt tacttttcca taattcatcc
     4261 ttgtatctcc ttatctcaaa gcttcggctc cgctaactac ctctaataac tctttagtta
     4321 tagatgcctg tctggctttg tttctttgta aggtgagatg ttcaatcatc tcctcggcat
     4381 tcttggttgc tcctcccata gctatcatac gtgaaccatg ctccgaggca aaagcctcag
     4441 ccaaagccat ttgaatcacg gttgaacaat acctcggcaa tagctcggtg aatatctcct
     4501 ccggctccgg ctcgaagatg tagtcaacca tttctttctc atctttgtct ttgctttcta
     4561 tgttcagaaa tttctgcaaa ttcacccgat aggacatagc ggaaatgaac ttggtatata
     4621 ggatatgtat ctcgtctgcc tctccgaata aaaacagttt agttaaatcc tcagatatgc
     4681 tcttgatgtt gcttgtaacc aaatttcctc ccaaactcaa atatttcagc ttgatttcaa
     4741 accctctctt cctgtaaaaa tcgtatcctt tcttacccac atttattatc atcacgtttt
     4801 gaggggtgta gtttttgata aatccctggg ccgctcgaat tatgttgctg ttataagagc
     4861 cgcacaatcc cctatccgaa gtaattacca ccaacgcaac tatctttgcc tccctttttt
     4921 caaacagagg gtgtgttatc tcttcgctgg cagatgataa atttaccagc atctcctgca
     4981 ttttaagggc ataggggcga gaggattcag ctttgctttg tgccctcctc aacttcaccg
     5041 ccgccaccat ctccatagct ttggtgatct cgtgtgtgct ttcaacgctt ctgattctct
     5101 ttcttatatc acctaaggtt tccattctat ttctctttca attgggctac aaattgtcct
     5161 ttaaacttct ttatagcttc atccaatttc ttctgggtgg tttcgtccaa atctttagtc
     5221 tgggcaatgg catgcaccac atccggatac tccttatcca taaaaggata aaactcctct
     5281 tcgaattttc ttatgacctc taccttcaaa tcgtccagat acccattcac tgcggtatag
     5341 attatcatca cctgtttttc cagaagcatg ggaacgtatt gttcctgctt tagaatttca
     5401 actgttcttt ccccacgggt tatctgtgcc cgagtggctt catccagctc cgttccgaat
     5461 tgggcgaaag cagccatttc acgatattgc gccaaatcca acctgagcat acctgccact
     5521 tttttcatac ttttggtttg ggcacttcct cccacgcgcg aaactgaaat tccgacattt
     5581 atggctggcc tgatacctgc atagaaaagg tctgattcca agaatatctg accatcggtg
     5641 atggagataa cgttagttga aatgtaagat gtgatatctc ctgcttgagt ttcgataatc
     5701 ggtatggcgg tcaatgagcc gcctcccaac tcgtcactta atttagccgc tctttccaaa
     5761 agtcttgaat gcagatagaa aatgtctccg ggataagcct ctcttcccgg aggtcttctt
     5821 aaaagtaaag ctaactgccg ataagccttg gcatgcttag agaggtcatc gtaaattacc
     5881 aaaacatgtt ttttgttgtc tctgaattcc tcacccatag cacaaccggc ataaggagca
     5941 atgtattgca atggtgctgg gtcagttgca cctgcaacca ccacggtggt gtattccatt
     6001 gctccgtaat cctctaaagt cttaaccacc tgtgccacag ttgaagcttt ctgacctata
     6061 gccacataaa tacaatagat atctgaattt ttttgattaa ctatggtatc caacaccaag
     6121 gccgtcttac cggtctgcct gtcaccaatt accaattccc tttgaccgcg accaattgga
     6181 atcatagaat caattgcttt taagccggtt tgcactggtt ccttgaccgg ctgtctttgg
     6241 accacgtttg gtgctccacc ttctattggc ctgaacttct tggtaacaat tggacctttg
     6301 tcgtctattg gttcacctaa agcattaacc actcgaccca acattacctc acccactgga
     6361 actgatgcta tcttgccggt cctcttaacc gcatctcctt cctttatatt tgtatccgag
     6421 ccgaatatca caactccaac attgtcttcc tctaaattca aaatcatccc ggtgatatca
     6481 ttgggaaact taatcagctc actcatcatc gcatcttcca atccccagat gcgtgcaata
     6541 ccgtccccca cctgcagcac cacccccaca ctctccatct gtaatttggt ctggtacttc
     6601 tcaatttcct tctggatgat taggctaact tcctctggtt tcaaagacat acatttctcc
     6661 ttatctaaga caccctaagc cctgacatct cctccttgag ttgcctcaag taatagctga
     6721 tgctatggtc aattattcga ttttccagta taagggccat accaccgata atggctggat
     6781 tgactttcaa gagcatttcg atttccttgc cggtcttttt ctccagtcta tcctgcagct
     6841 tcttggctat atcttttgag agaggcctgg cagtggtaac cattacctgt attattccct
     6901 tatgctcctt gactaatttt tcgtattcat ccgcaatagg gagcagatac tcaaagcgat
     6961 gccgattcaa aagcacaagc agaaaatggt aaaaaactgc atctaatttg ccggaaaagg
     7021 ccctccttag aaattcctct ttttcttttt ttgtaatttg aggaacttct aagaagcttt
     7081 tgaagtcagt atgctctcta agtaaagccg ctactgtttt ttgctctccc aacacccgct
     7141 ccagtgcgtc cttcttcaaa gcagccaaga atagtccgag agcatacctt tttgctatct
     7201 ctgggtttat cactctaagt tctccatctg attgatgaaa tcggaaatca gcttcttatc
     7261 tttcttttca tccattttct ctttcattat cttctccgcc gcacccaggc t
//
LOCUS       DASWLN010000151         3767 bp    DNA     linear   ENV 20-FEB-2024
DEFINITION  MAG TPA_asm: Candidatus Zixiibacteriota bacterium isolate
            SMAG_U2949 k141_757070, whole genome shotgun sequence.
ACCESSION   DASWLN010000151 DASWLN010000000
VERSION     DASWLN010000151.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37960594
            Sequence Read Archive: SRR10376091
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Candidatus Zixiibacteriota bacterium (soil metagenome)
  ORGANISM  Candidatus Zixiibacteriota bacterium
            Bacteria.
REFERENCE   1  (bases 1 to 3767)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 3767)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (26-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/12/2024 08:28:26
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 1,440
            CDSs (total)                      :: 1,416
            Genes (coding)                    :: 1,412
            CDSs (with protein)               :: 1,412
            Genes (RNA)                       :: 24
            rRNAs                             :: 1 (16S)
            partial rRNAs                     :: 1 (16S)
            tRNAs                             :: 21
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 4
            CDSs (without protein)            :: 4
            Pseudo Genes (ambiguous residues) :: 0 of 4
            Pseudo Genes (frameshifted)       :: 0 of 4
            Pseudo Genes (incomplete)         :: 3 of 4
            Pseudo Genes (internal stop)      :: 1 of 4
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..3767
                     /organism="Candidatus Zixiibacteriota bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_757070"
                     /isolate="SMAG_U2949"
                     /isolation_source="soil"
                     /db_xref="taxon:2053527"
                     /environmental_sample
                     /country="USA: Oregon"
                     /lat_lon="42.99 N 123.63 W"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            <1..679
                     /locus_tag="VGB16_05020"
     CDS             <1..679
                     /locus_tag="VGB16_05020"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_017213797.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=2
                     /transl_table=11
                     /product="amidophosphoribosyltransferase"
                     /protein_id="HEX9917080.1"
                     /translation="QAFCIFEFIYFARPDSMIFGENVDKIRRRLGRQLAREKPVDADM
                     VMSVPDSSNTATLGYSEQSGIKYELGLIRNHYIGRTFIHPEQKIRDLDALIKYNPVKG
                     VLRGKKVIIVDDSIVRGTTSRKLVKMIRHAGAKEIHFRVSSPPIISPCFYGIDMPTKK
                     ELIASSKSVEQIRKYLAVESLGYLSLEGMLSIQSLPKANFCTACFSGKYPTKVEMNNT
                     KFVLEKK"
     gene            1425..2783
                     /locus_tag="VGB16_05025"
     CDS             1425..2783
                     /locus_tag="VGB16_05025"
                     /inference="COORDINATES: protein motif:HMM:NF025885.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_process: GO:0006355 - regulation of DNA-templated
                     transcription [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="sigma-54 dependent transcriptional regulator"
                     /protein_id="HEX9917081.1"
                     /translation="MNPARIMVVDDEESMCKFMQIMLEKEGYQVTATQSSNYALEEIE
                     KDSYDLIIADLMMPEMSGIELLSRVKSIHPDLSFIVMTAFASVDTAIEALKNGAFDYV
                     TKPFKVDEVKIAIKKSLEHKRISKENVSLKKELRKEFSFANFIGDDPQIIKMKEMAKR
                     IAESESTILIQGESGTGKELVAKAIHYHSRRSDKPFVTINCAAIPESLLESELFGHVK
                     GAFTGAIRDKDGLLKVADEGTFFLDEIAITSPAIQMKLLRVLEEKEVTPVGGTKPINV
                     DVRLIAATNADLEQDVKLGNFRPDLFYRLNVIPITIPPLRERKDDIKLLAFYFIKKYC
                     AIRGVEEKTISERALEKLIACRWPGNVRELENTMERAVLLAQRKEIDLEDLPEKIREG
                     VPVDFVTETKPSSPTLEEIEKAYINWVLQEADWHKAKAAKMLGIDSSTLYRKIEKYKL
                     KV"
     gene            2965..3429
                     /locus_tag="VGB16_05030"
     CDS             2965..3429
                     /locus_tag="VGB16_05030"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_006965159.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="prepilin-type N-terminal cleavage/methylation
                     domain-containing protein"
                     /protein_id="HEX9917082.1"
                     /translation="MHILKTHLKTQKSWNSCKNKKGMTLIELMIVVVIIGVLAAIAIP
                     RFMNVSTKAKQSEAKGILKQIYINEETYLQSTQEVEYWIPSESASASNQKAFATIWID
                     VMPTARYTYAITGDSTFFVATATANLDEDSTVDEWQMDTSGVLNCISNDAQH"
     gene            3656..>3767
                     /locus_tag="VGB16_05035"
     CDS             3656..>3767
                     /locus_tag="VGB16_05035"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_014399068.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="prepilin-type N-terminal cleavage/methylation
                     domain-containing protein"
                     /protein_id="HEX9917083.1"
                     /translation="MLSKFHRNSKGFTLVELMIVVVIIGILAALAIPRFMR"
BASE COUNT         1189 a          699 c          906 g          973 t
ORIGIN      
        1 gcaagccttc tgcatattcg aatttattta ctttgcgcgt ccggacagta tgatttttgg
       61 agagaatgta gataagataa gaaggagatt gggcagacag ttagccaggg aaaaaccggt
      121 ggatgcggat atggttatgt cagttcccga ttcaagcaac accgccacct tgggttactc
      181 ggagcaatcc ggaataaagt acgaattggg gctgattcgc aatcactata ttggcaggac
      241 tttcattcat ccggagcaga aaataagaga tttagatgcg cttatcaaat ataatccggt
      301 caagggagtg ttaaggggaa agaaagtgat tatcgtggac gactcgattg tgcgaggcac
      361 caccagccgg aaactggtta agatgataag gcatgctggc gccaaggaga tacatttcag
      421 ggtcagttcc cctccgatca taagcccctg cttctatggt atagatatgc ccaccaagaa
      481 ggaattgata gcttcttcca aatcggtgga acagataaga aaatacttag ccgtagaaag
      541 tctgggatat ctttctttgg aggggatgct ctccatacaa tccttgccca aagctaactt
      601 ctgtaccgct tgcttctccg gaaaataccc caccaaagtc gaaatgaaca acaccaagtt
      661 tgttttagag aaaaaataag gatcatccta ctttggagtt ttcgatttgt agggacagaa
      721 cattgttctg tccctattta tttaggcctt accttatatt ctctccttaa cactaggaag
      781 aggaaactaa cttgttcccc atcctgatga aagagggata gggagaggtt tgttctctca
      841 aagctcaaaa ctatttgttg gacaggtcta aaggtctgtc tctaatttta aatcttaaac
      901 tcaaaactac tttattgtag cggagaccta aagatctcca tctgattctt atggaaagct
      961 aaagttttcc gctacaaacc caatagattc ccgctggtat gaaccaaaac ggttcatacc
     1021 gctggagttt atcctgcaaa atgtggggtg ggaatgacag ggtgaggatt tgtcattcct
     1081 gcgcaggcag gaatccacgc ttttgatata aacagattcg tgtttctata gaaatgacaa
     1141 ataagaaagc agtagtttat taagatcgca cctgaaaacc ccgtcctgtg gacacggcga
     1201 tgaaaggtgc gtctgctgga agcaaaagaa acagaccaga tactgaaacg agttcagcat
     1261 gacaacaggg tttggttccc ggtttttgtc aattttgggt tttaaagccg tacccacaat
     1321 gtgaggttct gtatctgttt ttttattctc tgaagttaga gaaaccttca gccgatatag
     1381 attatagagt attagacaaa actaatcttc caaaggcaag agatatgaat cctgcgcgta
     1441 ttatggtagt ggatgatgaa gagagcatgt gcaagttcat gcagatcatg ctggagaagg
     1501 aaggatacca ggtaactgcc acccaaagca gcaattacgc tctggaagag atagaaaagg
     1561 acagctacga tttgatcata gcggatttga tgatgccgga aatgagcggc atagagcttc
     1621 tgtcccgagt gaagtccatt catccggatc tcagtttcat cgtgatgacc gcctttgcct
     1681 cagttgacac cgcaatagaa gccttgaaaa atggagcttt cgattacgtg accaagcctt
     1741 tcaaggtgga tgaggtaaaa atcgcaatca agaagtccct ggaacataaa aggatatcca
     1801 aagagaacgt ctccttgaaa aaggagcttc gcaaggaatt cagcttcgcc aatttcattg
     1861 gagacgaccc acaaatcata aagatgaagg aaatggctaa gagaattgct gaatccgaga
     1921 gtactatatt gattcaagga gagagtggga caggaaaaga gctggtggcg aaagctatcc
     1981 attatcacag ccgcaggtcg gacaaacctt ttgtcactat aaattgcgca gccatacccg
     2041 agagtctatt ggaaagtgaa cttttcgggc atgtcaaagg ggcgttcacc ggggcaatca
     2101 gggataaaga tggattgttg aaggttgcag acgaaggaac ttttttcttg gatgagatag
     2161 caattacctc accagctatt caaatgaagt tactgcgggt gctggaggag aaagaggtaa
     2221 ctccggtagg cggcacaaaa ccaataaatg tcgatgtccg cttgattgcc gccactaacg
     2281 ctgacttaga gcaggatgtc aagctcggga atttcaggcc agacctgttt tatcgtctaa
     2341 atgtgattcc catcaccatc ccgcctctac gggagaggaa agatgacatt aagcttttag
     2401 ctttttattt cataaagaaa tattgtgcta tcaggggagt tgaggaaaag acaatttcag
     2461 aaagagcgtt ggagaaattg atagcttgtc gttggccggg aaatgtgcgt gaactagaga
     2521 acaccatgga gagagcagtg ctcctggctc aaagaaaaga gatcgatctg gaggatttgc
     2581 cggaaaagat cagagaagga gttccggtcg atttcgttac ggaaaccaag ccatcctctc
     2641 caactttaga ggaaatagaa aaagcatata tcaactgggt attgcaggag gcggattggc
     2701 ataaagccaa agccgccaaa atgctgggaa ttgacagctc aactctgtac aggaagatag
     2761 aaaaatataa gcttaaggtg tgatttagag ctttctaaag ggtttagaga atttagcaat
     2821 atgcaagatg ttaggcaaaa cgcaagcata ttggcgaatc gggaattatg gcagcgttgt
     2881 tatttgatta gatggtaaca agttacacga ctggtttttg ctagataggt tggcacaaaa
     2941 gttgccctaa taaattttaa gcatatgcat attctcaaga cacatctcaa gacacagaag
     3001 tcgtggaatt cgtgcaaaaa caaaaaggga atgaccctca tagagctaat gatagtggtt
     3061 gtgattattg gggttttggc agctatagct attcccagat ttatgaatgt atcgactaaa
     3121 gcaaaacaat cagaagccaa ggggatattg aagcagatat atataaatga ggaaacctat
     3181 ttgcagtcaa cccaggaagt cgaatactgg attcccagtg aaagcgcaag cgcaagcaat
     3241 caaaaagctt ttgcgaccat ctggattgat gtaatgccaa ctgcaagata cacctatgct
     3301 attactggtg attcgacttt tttcgttgcc actgctactg ctaatctgga tgaggattcc
     3361 actgttgacg aatggcaaat ggacacttcc ggggtcttga attgcataag taacgatgct
     3421 cagcattaac agattgcgtt ttgcaaagaa caaggcaaat tccactatat tatgagcgga
     3481 aagtgatgta tgaaagatta tcacctggtg atgtatcaat tgaaccacat acgattagaa
     3541 gatatcattc aatgttggca tattgtttgc ttagctgata tagtgcgact atagactata
     3601 atatagacag ttgggtaaaa agccgaaaac ttaaaagttg agggaggtga aaaaaatgtt
     3661 gagcaaattc cacaggaata gcaaaggttt caccctggta gagctgatga tcgtggtagt
     3721 tatcatagga atcttggccg cattggcaat tccgagattt atgagag
//
LOCUS       DASWLN010000152        12567 bp    DNA     linear   ENV 20-FEB-2024
DEFINITION  MAG TPA_asm: Candidatus Zixiibacteriota bacterium isolate
            SMAG_U2949 k141_202464, whole genome shotgun sequence.
ACCESSION   DASWLN010000152 DASWLN010000000
VERSION     DASWLN010000152.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37960594
            Sequence Read Archive: SRR10376091
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Candidatus Zixiibacteriota bacterium (soil metagenome)
  ORGANISM  Candidatus Zixiibacteriota bacterium
            Bacteria.
REFERENCE   1  (bases 1 to 12567)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 12567)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (26-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/12/2024 08:28:26
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 1,440
            CDSs (total)                      :: 1,416
            Genes (coding)                    :: 1,412
            CDSs (with protein)               :: 1,412
            Genes (RNA)                       :: 24
            rRNAs                             :: 1 (16S)
            partial rRNAs                     :: 1 (16S)
            tRNAs                             :: 21
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 4
            CDSs (without protein)            :: 4
            Pseudo Genes (ambiguous residues) :: 0 of 4
            Pseudo Genes (frameshifted)       :: 0 of 4
            Pseudo Genes (incomplete)         :: 3 of 4
            Pseudo Genes (internal stop)      :: 1 of 4
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..12567
                     /organism="Candidatus Zixiibacteriota bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_202464"
                     /isolate="SMAG_U2949"
                     /isolation_source="soil"
                     /db_xref="taxon:2053527"
                     /environmental_sample
                     /country="USA: Oregon"
                     /lat_lon="42.99 N 123.63 W"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            <1..621
                     /locus_tag="VGB16_05040"
     CDS             <1..621
                     /locus_tag="VGB16_05040"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEX9917084.1"
                     /translation="LPETQPMSYAALKHLFKKNIKVITLTTVPLGALSVMEGVVDSVA
                     GEYHKKYGVDYVNLGYKYGSDAVMLGMGKKISDIFPQDNYDNPLEDLPLMQKVKNYRN
                     VDFIFIVSDNATVDYWVSLVNARFGTPMGAGVTAVMAPKCYSYLQTKQLVGLLGGMKG
                     AAEYEKLSKNYGRAMVGMDPQSIIHLLIIFFIVIGNIGYFVTKKSK"
     gene            624..1229
                     /locus_tag="VGB16_05045"
     CDS             624..1229
                     /locus_tag="VGB16_05045"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_020250985.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEX9917085.1"
                     /translation="MNWEFFGTSLAALLTLAIYSFLYKDNPFYKVAEHVFAGISAGYY
                     IGLIWHSVIQQQLISPLIIHGKYLLIIPGILGILMFSRFFPKIAWVSRISLAFVIGTT
                     AGIYLVMQFHGLIIPQVRSTFLSFSAFSSIVMVVGVISTLIYFYFSKEHKGALGLVAQ
                     VGIWFIMIAFGASFGYTVMARVSLLIGRAQFLIIDWLKIIR"
     gene            1658..2629
                     /locus_tag="VGB16_05050"
     CDS             1658..2629
                     /locus_tag="VGB16_05050"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_012501103.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016779 - nucleotidyltransferase activity
                     [Evidence IEA];
                     GO_process: GO:0009058 - biosynthetic process [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="sugar phosphate nucleotidyltransferase"
                     /protein_id="HEX9917086.1"
                     /translation="MKVIIPVAGIGARLRPHTHTDPKALLHVAGKPILGHILDRVKHL
                     KTSQVIFVIGFMGEDIKRYVRMNYKFKCSFVEQEELKGLGYAIYLACRKIKKDEPLLI
                     ILGDTIVESDLSRVVKSKYDALGVRKVEDPRRFGIVELKSGWAKKVVEKPDKPTSNLA
                     LVGIYYIKSVSLFKNCLEQVIRKGIKTKREYQLTDALELMIKKGVKFSVFNVKGWYDC
                     GKPETLLETNRHLLSKAKTRPILKGAILIPPVYVSGFARVCNSVIGPYVSIADDAFIK
                     NSVIRNSIIGKKAIVNGCLLESSLVGTGAEVNGVFNRFNVGAWSEIG"
     gene            2958..3902
                     /locus_tag="VGB16_05055"
     CDS             2958..3902
                     /locus_tag="VGB16_05055"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEX9917087.1"
                     /translation="MESPSSKPLSFSDYMRGVWVNKDRELVITDKFLIDFGIDANGGI
                     WAKLSLLEYKNSSLTIDTRGVVYAHGKDKVMKVCLKDIFGKSDSLVFNTNKEMESFIR
                     KEMFNMKRIDNKEIVLNSSSEKMIATKDGSFEIFKNDSLLDSLFRIEHFDISEDYMPT
                     KKLSESTIKDCLVNWTLRSEVIKNSKGNNVGLEINTREHMYIFYVLNLDTVYCRAARY
                     ATSNKGVVFDQNIRASLIGGEWSVFSAKDNTIAAEKKSVDESLFNAEACAFTKSGIYW
                     SIRSATDDLIELNGCGGETYKWERPIKENSSDIVEWIK"
     gene            3935..5098
                     /gene="metK"
                     /locus_tag="VGB16_05060"
     CDS             3935..5098
                     /gene="metK"
                     /locus_tag="VGB16_05060"
                     /EC_number="2.5.1.6"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_020619511.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004478 - methionine adenosyltransferase
                     activity [Evidence IEA];
                     GO_process: GO:0006556 - S-adenosylmethionine biosynthetic
                     process [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="methionine adenosyltransferase"
                     /protein_id="HEX9917088.1"
                     /translation="MSKGNFFFTSESVTEGHPDKVCDQISDAVLDDLIKQDPKCRVAC
                     EAFITVGLVIVGGEITTTGYSDIGKLVRNLIKDIGYTHSKYGFNYETCAVLNAIGSQS
                     PDIAQGVDVGGAGDQGLMVGYASRETPELMPMPIMLAHKLCMRLAEVRRKKILKYLGP
                     DGKSQVTVQYVDGKPQRVDAVVVSSQHTEEILDQTGKKITEEARQEMIDNVVKPVIGE
                     KLLDSKTKYFVNPTGKFLIGGPQSDTGMTGRKIIVDTYGAMAPHGGGAFSGKDPTKVD
                     RSATYMARYIAKNIVASGLADKCTVQLAYAIGLAEPISIMINTHDTGKVPNKRIVELI
                     KKHFELTPQGIIRRLDLIRPIYRRTAAYGHFGRENADFTWEKIDKAGELKKDA"
     gene            5154..5819
                     /locus_tag="VGB16_05065"
     CDS             5154..5819
                     /locus_tag="VGB16_05065"
                     /inference="COORDINATES: protein motif:HMM:NF013921.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003677 - DNA binding [Evidence IEA];
                     GO_function: GO:0004803 - transposase activity [Evidence
                     IEA];
                     GO_process: GO:0006313 - DNA transposition [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="transposase"
                     /protein_id="HEX9917089.1"
                     /translation="MLHRNSQKRFYVENSVYFITTKTYDSFPYFDEEILCNLLIDSLK
                     YSQKKKSFKIFGYKINPDHIHLLIQSNNEHNYSKIMHNVKRNFSHNANIVLGVHEVDC
                     EIKGDDIASKGECGVKGDDYGINKGDDNYRRLCGHGYANYKKLWNYDILVKYKNRLDD
                     KFHNNHPYPKFRWQKSFHYHIIVDARDVLNHVKYIKNQYIKHHLNGNAHCFINDELIQ
                     EVV"
     gene            5928..7184
                     /gene="ahcY"
                     /locus_tag="VGB16_05070"
     CDS             5928..7184
                     /gene="ahcY"
                     /locus_tag="VGB16_05070"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_013537999.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004013 - adenosylhomocysteinase activity
                     [Evidence IEA];
                     GO_process: GO:0006730 - one-carbon metabolic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="adenosylhomocysteinase"
                     /protein_id="HEX9917090.1"
                     /translation="MRYDIKDIGLASKGNLRIEWSERNMPVLRLIRERFAKEKPLSKI
                     RIAACLHVTTETANLVRTLVAGGAEVVLCGSNPLSTQDDVAACLVKDYGIPVFAIKGE
                     SNKIYYQHIQSALEKNPHITMDDGADLVSTLHTKKSKLAKEIIGGTEETTTGVVRLRS
                     MEKNNVLKYPIIAVNDSKTKYFFDNRYGTGQSTIDGILRATNVLLAGAKVVVAGYGWC
                     GRGFAMRAKGMGADVIVTEIDPLKAIEAIMDGYRVMSMSVASRIGDVFCTLTGDINVI
                     RPEHFKMMKDNAVVCNSGHFNVEIDIPGLEKMATKKRRIREFVDEYTLKNGKRVNLLA
                     EGRLVNLSAAEGHPACVMDMSFANQALSAEYLVKYGKNLKKKVYIVPEKIDSQIAALK
                     LKSMGVRIDRLTPEQKKYLASWEMGT"
     gene            complement(7487..8578)
                     /gene="lptG"
                     /locus_tag="VGB16_05075"
     CDS             complement(7487..8578)
                     /gene="lptG"
                     /locus_tag="VGB16_05075"
                     /EC_number="7.5.2.5"
                     /inference="COORDINATES: protein motif:HMM:TIGR04408.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0005886 - plasma membrane [Evidence IEA];
                     GO_function: GO:0015221 - lipopolysaccharide transmembrane
                     transporter activity [Evidence IEA];
                     GO_process: GO:0015920 - lipopolysaccharide transport
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="LPS export ABC transporter permease LptG"
                     /protein_id="HEX9917091.1"
                     /translation="MSILDKYILRQFRNALLFLFLAVWTVFLLVDLINQLDKFMDQHA
                     SLFSVIKYYLFYSPYIIVLTLPIVVLLATLLSVGQLSRKNEITAMKSVGISLYRILSP
                     ILAMGLVISLVIMVVGVVVLPYTDEKKLEVKRVEIEKGAPYTGGNLYNLLVQDQSGAI
                     FSLKRYNSQKKFGTDVLVQKFRDGHLLERIDAQKMSWTETGWLLERGHYRIFSDSLDL
                     PHLEEYKTFDRLLRLDFKIKPEAFAKREKMTEEMSFKELADYINAKKETKQEVPKELT
                     DLYFKISYPFISLIVVLIAAPLAANPRRSGLAISFALGLFISFAYYILLQIFRSLGYS
                     QKLTPLLAVWLANIIFLIVGMVILMKAEK"
     gene            complement(8585..9946)
                     /locus_tag="VGB16_05080"
     CDS             complement(8585..9946)
                     /locus_tag="VGB16_05080"
                     /inference="COORDINATES: protein motif:HMM:NF015684.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0042626 - ATPase-coupled transmembrane
                     transporter activity [Evidence IEA];
                     GO_function: GO:0140359 - ABC-type transporter activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="LptF/LptG family permease"
                     /protein_id="HEX9917092.1"
                     /translation="MKILERYIVREHLGPFFFALAVIMFVLVMDFILKVVNLIIGRGL
                     NAYVILQVFVLNLAWMIALAVPMAVLVATLMAFGRLSEDNEITALKASGVSIYRTIAP
                     ALGLGLVLMIGLILFNNEVLPDANQRARLLQSDITQKRPTWSLKENVFMDEIPGYHIL
                     VNHIDPHSSKIKGITIYDQKDRKLPRTIIAKRGELEFAPDGNTLILHLEDGEVHESDE
                     EEPGLYRRMAFEKQTIYFTDVGSQLVRRSSEYRTDREMSSKMMLEQARRIEQSIKRKE
                     DKSRELVSGLVDPIFPPSLYVTDYPIGSGLDTATIINQLLNKNGQALTELSNERARIM
                     ANRKEKNRYLVEIHKKYSIPVACVVFVLIGAPLGIISRKGGIGAAAGLSLGFFVLYWA
                     FLVGGEELADRLVISPFWAMWSANILTGFFGIYILVSSTREAFYIPWEKLRKIIPHGW
                     RKS"
     gene            10469..11437
                     /locus_tag="VGB16_05085"
     CDS             10469..11437
                     /locus_tag="VGB16_05085"
                     /inference="COORDINATES: protein motif:HMM:NF014069.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="HD domain-containing protein"
                     /protein_id="HEX9917093.1"
                     /translation="MKKLFVKDLKPGIEVVDLFVLRKKELKESDSKKRLQLELGDKSG
                     RIDAIAWDEPVQFYELAQEGDVVKIEGWVGTYQGKPQIKLKTLTKLRTGDFKLEDLLP
                     SSLEDLDALLEKFKQKILSIQNQYLKSLFADLTGDVEFMNRLRVAPAGKLWHHSCLGG
                     LLEHTLRVTSLCELAAQNYDLIDRDLLVSGALLHDIGKITEYSVSGFIEYTDEGRLIG
                     HVVSGDKLVASRLEKIDGFPEELALRLRHLVLSHQGKLECASPVVPKTIEAIVLHYAD
                     ELDAKADAFTRVINKSKAAGKKWSEWVGLIDRYIYIGENVLEDTEE"
     gene            11437..12465
                     /locus_tag="VGB16_05090"
     CDS             11437..12465
                     /locus_tag="VGB16_05090"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_010251440.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0005737 - cytoplasm [Evidence IEA];
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_process: GO:0000902 - cell morphogenesis [Evidence
                     IEA];
                     GO_process: GO:0008360 - regulation of cell shape
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="rod shape-determining protein"
                     /protein_id="HEX9917094.1"
                     /translation="MSLIDLFSNDIGIDLGTANTLVFIRGAGIVLNEPSVVAIEVSTK
                     KVLAIGRVAKEMIGRTPGEIVAIRPLKDGVIADFEITERMLADFIRRVVKHRYLMKPR
                     IVISVPSGITEVEKRAVRDSAENAGAREVFLIQEPMAAAIGVGLPVEQPSGSMVIDVG
                     GGTSEIAVIALHGIVTNTSIRIAGDEMDEAIVFYLKKNYNLLIGDRTAEEIKIKIGTA
                     YPQDEEESIEIRGRDLVAGIPKTMKISSVQVREALSEPVSAIVDAVKLTLEQTPPELS
                     ADILDRGIILTGGGALLKGLDQRLREETSLPVNVADDPLTCVARGTGKVLEDMSRYAK
                     VLMRGRKE"
BASE COUNT         3797 a         2396 c         2989 g         3385 t
ORIGIN      
        1 ctgcccgaga cccagccaat gtcctatgcg gccttaaagc atcttttcaa aaagaatata
       61 aaagtcataa ccttaaccac agtcccctta ggggcgcttt ccgttatgga aggcgtggta
      121 gatagtgtgg ctggtgaata ccataagaaa tatggcgtgg attatgttaa tttaggatat
      181 aaatatggtt ctgatgcggt tatgttaggt atggggaaaa agatttcgga tatatttcct
      241 caagacaatt atgataatcc cttggaagac cttcccctga tgcaaaaggt gaaaaattac
      301 aggaatgttg atttcatatt catagtttca gataacgcca cagttgacta ttgggtctcc
      361 ttggtgaatg cccgttttgg aactcctatg ggagcaggag taactgcggt tatggcaccc
      421 aaatgctatt cctatctcca gaccaagcag ttagttgggc ttttaggtgg aatgaagggc
      481 gcagcggagt atgagaaatt gagtaaaaat tatggtaggg cgatggtggg aatggaccca
      541 caatcgataa ttcacctttt gattatattt ttcatcgtaa tcggaaatat aggatatttc
      601 gtaaccaaaa agagcaaata ataatgaatt gggaattttt cggaacaagt ttagccgcgc
      661 ttcttacctt ggctatctat agctttttat acaaggacaa tccattctat aaggtggctg
      721 aacacgtctt tgccggaata tcagcaggat attatatcgg cttaatctgg cacagcgtca
      781 ttcaacagca actcatctcg cctttaatta tccatggtaa atatcttctg attattccgg
      841 ggatattagg aattttgatg ttttcgcgct tctttcccaa gatcgcctgg gtttccagaa
      901 tatctttggc tttcgtgatt ggaactaccg cggggatata tctggtgatg caatttcatg
      961 gtttaatcat tccccaagtc agaagcacct tcctatcctt tagtgctttc tccagtatag
     1021 taatggtggt gggagtaatt tccactttaa tatattttta tttctccaaa gagcataaag
     1081 gggcgctcgg tctggtagct caagtgggaa tatggttcat aatgatcgcc tttggagcat
     1141 cctttggata tacggttatg gcgcgagtat cgcttctcat tggcagagca caatttttga
     1201 taatagattg gctgaaaata ataaggtgaa atcgtataga ggatagtaga tcggtcgggc
     1261 aaggtgcccg acctacgaaa agtgaacaac ttttttgttt tgtcaggacc tcccttcgac
     1321 tctgctcagg ccttcggctc tgcacttcgt cctgcccttc ggtcccgaga tctcagggtc
     1381 gagggaaacc tcagtgtcga aggaaggctc aggctcgaag agatggtgag gacattcggc
     1441 tgagcggttc gaccgtcttg agcggctcac cgtcgaagga ctcagtcgaa gcccatgtcg
     1501 aaccagagtc ctgacagcca gattgtgcgt caggaatggc tcctaacgca acagtacatt
     1561 cccgtaggta tggaaccata tagtttacat cgtaggcagg gctcccagcc ctgcaaaaag
     1621 actttttcaa caatctcaaa ataaatgagg ggagagcatg aaagtaataa ttccggtagc
     1681 aggcataggg gcaaggctta ggccacatac ccacactgac cccaaggcgc tattgcatgt
     1741 ggcaggtaag ccgattttgg gacatatcct ggatagagta aaacatctga aaaccagtca
     1801 agtcatcttc gtcatcggtt tcatggggga ggatataaaa agatatgtaa ggatgaatta
     1861 caagttcaaa tgcagtttcg tggagcagga agagctcaag ggattggggt atgctatcta
     1921 tttggcttgt cgtaagataa aaaaggatga gccgttgcta ataattctgg gcgataccat
     1981 tgtggaaagc gacctgtccc gggtagtgaa aagtaaatat gatgccttgg gagtgagaaa
     2041 agttgaggac ccgagacgat ttggcattgt ggagttgaag agtggttggg ctaagaaagt
     2101 agttgagaag ccggacaaac caaccagcaa tttagccctg gtgggaatct actatatcaa
     2161 gtcagtttcc ttgttcaaga attgtttgga gcaggtcata agaaaaggca ttaagaccaa
     2221 aagagaatat cagttgaccg acgctttgga gttgatgata aagaaaggtg ttaaattttc
     2281 agtcttcaat gtcaagggat ggtacgattg cggcaagccg gaaacgcttt tggaaaccaa
     2341 tcgccatctt ttgtccaaag ccaaaaccag gcccattcta aaaggggcaa tcctgattcc
     2401 gccggtctat gtgtcgggct tcgctcgtgt gtgtaattcc gtaatcggtc cctacgtttc
     2461 tatagcagat gatgctttta ttaaaaattc agtcatccgt aattccatca tcggcaaaaa
     2521 agccatagtc aatggatgtc ttttggagtc atctctggtt ggaaccgggg cagaggtgaa
     2581 tggcgttttt aatcggttca atgttggtgc ttggtcagag ataggataaa ataaatttaa
     2641 aatttaaaat gacaaattaa aagataaaaa tggaatataa caattctaga ggaagaacgt
     2701 gagcatgggc aatttctttg tttgaggtat gattaaacaa tttttgttgt cattcctgcg
     2761 aaggcaggaa tccagaaaac aataaaaatg aataatgaca aaaaaactgg attccgcatc
     2821 aagtgcggaa tgacagacta ttcgtgagtc atcttcgttc ctgttaagtc ctattacttg
     2881 ggaggagaaa gtgtcagtag taactcctga cacaagaaaa gatagctgta gattactatt
     2941 tgtacttgga ggataagatg gaatcccctt cttctaagcc gcttagcttc tctgactata
     3001 tgcggggtgt ttgggtaaat aaagacagag aattagttat cacggataag ttcttaatcg
     3061 atttcggaat agatgcaaat ggcggaattt gggctaagct ttccctctta gaatacaaaa
     3121 atagcagttt gactatagat acccgaggcg tagtatacgc acacgggaaa gacaaagtta
     3181 tgaaggtatg cttaaaggat atctttggca aaagcgattc actggtgttt aatactaaca
     3241 aagagatgga gagtttcata agaaaagaaa tgtttaatat gaagcggata gacaacaagg
     3301 agattgttct caatagttct tcggaaaaga tgatagcgac caaagatgga tcgttcgaga
     3361 tattcaaaaa tgactcattg ttagattctc tatttcgaat agagcatttt gatatatcag
     3421 aagattatat gcctactaag aaactatccg aatccactat caaagattgt ctggtgaatt
     3481 ggacattgag gagtgaagtg ataaaaaata gtaaagggaa caatgtcggc ttggagataa
     3541 acaccagaga gcatatgtat atcttctatg ttcttaatct ggacaccgtt tattgtaggg
     3601 cagctcgata tgcgacctca aataaagggg tagtttttga tcaaaatatt cgcgcaagcc
     3661 taattggagg tgagtggagt gttttttctg caaaagacaa tacaattgcg gcagagaaga
     3721 aaagtgtgga tgaaagcttg ttcaatgccg aagcttgtgc gtttactaag tcgggaattt
     3781 actggtcaat aagaagtgca acggatgatc ttatcgagct gaatgggtgt ggtggggaaa
     3841 catacaaatg ggaaagaccc ataaaagaaa attcatcaga tattgttgaa tggataaaat
     3901 aactaatgag tctaaacaaa aacaaggaaa ggatatgagc aaaggtaatt tctttttcac
     3961 ttcagaatca gtaaccgaag gacatccgga taaggtatgc gaccagatat cagatgcggt
     4021 cttggatgac ctgataaaac aagaccccaa atgtcgggtg gcttgcgagg ctttcatcac
     4081 cgtgggtttg gttattgtcg gcggtgaaat caccaccaca ggttattccg acattggtaa
     4141 attagttaga aatctaatta aagatattgg ctatactcat tccaagtacg gcttcaatta
     4201 cgaaacctgt gcggttttaa atgctatcgg ctcacagtct ccagatattg cccaaggggt
     4261 tgatgttgga ggagctggcg accaagggtt aatggttgga tatgcctcta gggaaactcc
     4321 ggagcttatg cctatgccca taatgttagc ccacaaactt tgcatgagac tggcggaagt
     4381 cagaagaaag aagatactga aatatttggg accagatgga aaatcgcagg ttacagtcca
     4441 gtatgttgac ggaaaaccac aaagggtgga tgcggtggtg gtctcctcac aacacactga
     4501 agagatttta gaccagaccg gcaagaaaat aacagaagaa gcaagacagg agatgatcga
     4561 caatgttgtc aagccggtga ttggagagaa gcttctggat agcaagacca agtatttcgt
     4621 taaccctact ggaaaatttc tgattggtgg gccacagtcc gacaccggga tgaccggcag
     4681 gaagattata gtggatactt acggagcaat ggctccgcat ggcggagggg ctttttcagg
     4741 caaagacccg accaaagttg accgttcagc tacatatatg gcacgttata ttgccaaaaa
     4801 tattgtggct tctgggttgg cggataaatg caccgtgcag ttagcttatg ccataggtct
     4861 ggctgaaccg atttcaatta tgataaatac acacgatacc ggaaaagtgc cgaacaaaag
     4921 aatagttgag ctaatcaaga agcatttcga gcttactccc caggggataa tcaggaggct
     4981 ggatttaata aggcccatct accgccgaac tgcagcttat gggcattttg gaagagagaa
     5041 cgccgatttc acctgggaga agatagataa agcaggggaa ttgaaaaagg atgcgtgaaa
     5101 tataaaggcg atgataatta tcatcgcctt tatgaacgga aagacatata aaaatgcttc
     5161 acagaaattc gcaaaaaaga ttttatgtcg aaaatagcgt atattttatt actacgaaaa
     5221 catacgatag ttttccgtat tttgacgaag aaatattgtg caatttattg attgatagcc
     5281 ttaagtattc gcagaaaaag aaatcattta agatatttgg atataaaatc aatcctgacc
     5341 atatacattt attaatacaa tcgaataacg agcataatta ttcgaaaatc atgcataacg
     5401 tgaaaagaaa tttctcacat aacgcgaata tcgttttggg agttcatgag gttgattgtg
     5461 aaattaaagg cgacgatatt gcatcaaaag gcgaatgcgg tgtcaagggc gatgattacg
     5521 gcataaataa aggcgacgat aattatcgtc gcctttgtgg gcatggatat gcaaattaca
     5581 aaaaattatg gaattatgat attttagtga aatataaaaa tagattggat gacaaatttc
     5641 acaataatca cccataccca aaatttaggt ggcaaaaatc gtttcattat cacattatcg
     5701 tagatgcgag agatgtttta aatcatgtta aatacatcaa aaaccaatac ataaaacacc
     5761 atttaaacgg caatgcgcat tgctttatta atgatgaatt aattcaagag gttgtgtaat
     5821 caaatagtca tgttaattaa atgataaagg cgatgataat tatcatcgcc tttatgaaaa
     5881 gcaaacttgg ataacaacca cacacgacac aaacaaggag aagaatcatg agatatgata
     5941 tcaaagacat aggcttggcc agtaaaggaa acttgcgaat tgaatggtcc gagaggaata
     6001 tgccggtctt aaggttgatt cgagaaagat ttgccaaaga gaagccatta tctaaaatta
     6061 gaattgcggc ttgcctgcat gttaccactg agaccgcaaa tctggtgcgc acactggtag
     6121 ctgggggagc agaagtggtc ctgtgtggct ccaatccact ttcaactcag gatgatgttg
     6181 ccgcctgtct ggtcaaagat tacggcattc cggtatttgc catcaaaggc gaatcaaata
     6241 aaatttatta tcagcatatc cagagcgctt tggagaaaaa tcctcatata actatggatg
     6301 atggagcaga tttagtctca accttgcaca ctaagaaatc taaacttgcc aaagaaatca
     6361 ttggaggaac ggaagaaaca accaccggtg tggttagact gcgaagcatg gagaaaaaca
     6421 atgtcttaaa atatcccata attgcggtga atgattccaa gaccaaatac tttttcgata
     6481 accgctacgg aaccggtcaa tccacaattg acgggatctt gcgcgccacc aatgtgcttt
     6541 tagcaggtgc gaaagtggtg gtagcaggtt acggctggtg cggaagggga tttgcaatga
     6601 gggctaaggg aatgggagcg gatgtgattg tgaccgaaat tgacccttta aaggcaattg
     6661 aagcaattat ggatgggtat cgggttatgt ctatgtcagt tgcctccaga attggtgatg
     6721 ttttctgcac cttgactggt gatattaatg ttattcggcc ggaacatttc aagatgatga
     6781 aagataatgc agtagtttgc aattcgggac atttcaacgt ggaaatagac attcccggct
     6841 tggaaaagat ggcaactaaa aagagaagaa tcagggaatt tgtggatgaa tataccttaa
     6901 agaatggtaa aagagtgaat ctattggctg aaggaaggtt ggtcaacctt tcagctgcag
     6961 aggggcatcc agcctgcgtg atggatatga gctttgccaa tcaggcgctt tccgctgagt
     7021 atctggtgaa atacggcaag aatctgaaga aaaaagttta catagtacca gaaaagatag
     7081 attcccaaat cgccgctttg aaattgaaat ctatgggagt aagaattgat agattaaccc
     7141 cggagcaaaa aaagtatctg gcttcgtggg agatgggaac gtaaagacag tttaagagtt
     7201 taccctgcac catacggtgc agagtttaag agtttaggag tttatgatat tatgtaggga
     7261 caggtcgcga cctgtcccta ccctaattaa ttcttgtttt gttgtcattc ctgtgtaaac
     7321 aggaatccat ttggttttgt tcaaaaatgt agattcccgc tttcgcggga atgacaagat
     7381 atgaggaata tttttttgga gtgcgtccag ccgatggcgg gatattgcat tgacacggcc
     7441 aatacattcc aaggtaggcg atattcgcga atgtcgccta cgagaatcac ttctccgctt
     7501 tcatcaaaat caccatcccc acaataagaa agatgatgtt cgccagccag acggcaagaa
     7561 gtggagtgag tttttgactg tatcctaagg agcggaatat ctgcaagagg atgtaatagg
     7621 caaaactgat aaaaagaccc aatgcgaagc ttatagccaa cccactgcgg cggggattgg
     7681 cagctaaagg tgctgcaata aggactacaa tgagacttat gaaaggatag gatatcttga
     7741 aataaaggtc agtcagctct tttggaactt cctgcttggt ctccttcttg gcattgatgt
     7801 aatcagccaa ttctttaaag ctcatctcct cagtcatttt ctccctttta gcgaaagcct
     7861 ctggttttat cttaaaatcc agtcgcaaga ggcggtcaaa agtcttgtat tcctccaaat
     7921 gaggcaaatc caatgaatca gaaaagatcc gatagtgacc tctttccaga agccagccag
     7981 tttcggtcca actcatcttt tgtgcatcta tccgctccag aagatgtccg tctctgaatt
     8041 tctgcaccaa gacgtcggtt ccaaatttct tttggctgtt ataacgtttt aaactgaaaa
     8101 tcgcaccgct ttggtcttgc accaaaagat tgtaaagatt tccccctgta tagggagctc
     8161 ccttttctat ctccaccctt tttacttcca gctttttctc gtcagtataa ggtaagacca
     8221 ccactcccac caccataatc accaagctga taactaaacc catggctaaa atgggtgaga
     8281 gtatccgata aaggcttatg cccacagatt tcatggcagt tatctcattc ttgcgggaaa
     8341 gctgtcccac ggagagaaga gtggctagta acacaacaat gggtagagtt aagactatta
     8401 tgtaagggga ataaaaaagg taatacttga tcaccgagaa gagggaagcg tgttgatcca
     8461 taaatttgtc caattggtta atcagatcaa ccaggaggaa gaccgtccac acagctaaaa
     8521 ataagaaaag tagagcattc ctgaactgcc ttaggatata tttatctaaa atcgacattt
     8581 cccctcaact ttttctccat ccgtggggta ttatttttct caatttctcc caggggatat
     8641 agaaagcctc tcgggttgag ctgaccagga tgtaaattcc aaagaagccg gttaagatgt
     8701 tagctgacca catagcccag aaaggagaaa tgaccaacct atcagctaac tcctcacctc
     8761 ctaccagaaa agcccagtag agcacaaaaa atcccaggct cagtcctgcc gccgctccga
     8821 tccctccttt acgcgatatt attcccagag gagcgccaat caaaacaaaa actacacagg
     8881 caaccgggat ggaatatttc ttatgtatct cgactaaata tctgtttttc tcttttcggt
     8941 tagccataat tcttgctctt tcgttgctca gctcagttaa ggcctggcca tttttattaa
     9001 gaagctgatt tattatcgtg gctgtgtcca gaccgctacc tatcggatag tcagtcacat
     9061 agaggctggg cggaaaaatt ggatccacca agccagagac gagctctctt gatttgtctt
     9121 ctttacgttt aatgctttgt tcaattcttc tggcttgctc cagcatcatc ttggagctca
     9181 tctccctatc ggtcctgtat tctgagcttc ttcgcaccag ctggctgccc acgtcagtga
     9241 agtagatggt ctgtttttcg aaagccatcc ggcgataaag gcccggttcc tcctcatccg
     9301 actcatgcac ctctccatct tccagatgca gaataagggt attgccatcc ggggcgaact
     9361 caagttctcc ccttttagca attatggtgc gaggaagttt ccgatctttt tggtcataga
     9421 tggttatccc ctttatcttg gaggaatgag ggtcgatatg gttaaccaaa atatgataac
     9481 ccggaatttc atccataaat acattttcct ttaaactcca ggtgggcctt ttttgggtga
     9541 tgtccgactg caaaagtcgg gctctttgat tagcatccgg cagcacttcg ttattaaaga
     9601 gtataagtcc tatcattaaa accaaaccca aacccaaagc cggggcaatt gtcctgtata
     9661 tgctgactcc actcgccttt aaagcggtaa tctcattatc ctcggaaagc cttccaaaag
     9721 ccatgagggt agccaccaga acagccatgg gcacagccaa agctatcatc caggctaaat
     9781 tcagaacgaa aacctgcagg ataacataag cgttcaatcc tcggccgatg atgaggttga
     9841 ccaccttgag gatgaaatcc attacgagca caaacattat caccgccaag gcgaaaaaga
     9901 agggacccag gtgttctctt acgatgtaac gttcaagaat cttcatatta acaaaatata
     9961 agaaagccct cgggttttaa caaggttttt tcgagaactt ttcccaagaa tccgccttag
    10021 cttcttctga tctcgtgtaa atcagtgaaa taagtagact gaaatctcaa gaaggactct
    10081 gtttttgatt ttcaagtgag agtggtttgt ttaaagatta tttcctatcc cttcaaggga
    10141 gaggattaag ttgaaaatcc cgccagaggc ggagtgaggg tgaaagtcct tttctcgtat
    10201 ggtagtgtcg tagatgggga ataatgagag gcgctacggt tataaggctt aaactttttc
    10261 cccctcaccc caaccctctc ccctgtgggg agagggagaa ctaacaagga tgctatttat
    10321 ccctctggta tgaaccgtaa tgcttcatac ctctggggaa aatcctatcc atgctttcat
    10381 aggtcgggaa acccacggtt ttttttctgc ttgacatcct ttgagatttt acattataat
    10441 taaaagctaa gttgcatctc tttatcttat gaaaaaacta ttcgtcaaag atctaaagcc
    10501 gggcatagaa gttgttgatc tctttgtcct gcgcaaaaag gaacttaaag aaagcgattc
    10561 caagaaacgc ctccagctcg aactgggaga taaatctggc aggatagatg caattgcctg
    10621 ggatgagccg gtgcaattct atgagttggc ccaagaaggt gatgtggtta aaatagaggg
    10681 gtgggttgga acgtatcaag gaaaaccaca gataaaactg aaaactttaa ctaagctccg
    10741 aaccggtgat ttcaaattag aggatctgct tccctccagt ttggaggatt tggatgctct
    10801 tttggagaag ttcaagcaaa agattctatc cattcagaat caatatctca aatccctttt
    10861 tgccgatttg accggtgatg tcgaattcat gaataggtta agagtggctc cagctgggaa
    10921 gctgtggcat cactcctgtt tgggaggatt attggaacac actttgagag ttacctccct
    10981 ctgtgaattg gctgcgcaga attacgattt gatagacagg gatttgttgg ttagtggagc
    11041 tttactgcac gatatcggca agataacgga atattcggtt agtggattca tcgaatatac
    11101 ggacgaggga aggcttattg ggcatgtcgt ttccggggat aagttagtgg cttcccgcct
    11161 tgaaaagata gatggttttc cggaggagtt ggctttgagg ttgaggcatc tggtgttgtc
    11221 ccatcaaggc aaattggaat gcgcctcccc ggttgtcccc aaaaccatag aggcgatagt
    11281 gctgcattat gcggacgagt tagatgccaa ggcagatgcc ttcacgcggg tgataaacaa
    11341 atctaaggcg gctggcaaaa aatggagcga atgggtgggc ttaatcgacc gatatattta
    11401 tataggagaa aatgttttgg aagatacgga ggaataatgt ccttaatcga cctgttttcg
    11461 aatgatattg ggattgattt gggaacagct aatactctgg ttttcatcag aggcgcgggg
    11521 atagtactga atgagccatc cgtggtggca attgaggtct ccaccaagaa ggtcttagcc
    11581 ataggtcgag ttgccaagga gatgataggc aggactccgg gagagatagt ggctattcgc
    11641 cctttaaagg atggagtcat cgccgatttc gagataaccg agaggatgtt agctgatttc
    11701 attcgaaggg tagtaaaaca taggtatctg atgaagccgc gcatcgtcat ctccgttcct
    11761 tctggcataa ccgaagtgga gaaaagggca gttagagatt cagctgagaa cgcgggtgcc
    11821 agggaggtgt ttttgattca ggagccgatg gcggcagcca ttggagttgg tttgccggtt
    11881 gagcagcctt ccggcagcat ggtcatcgat gtgggtggtg gtacttcaga gatagcagtt
    11941 atcgctttgc acgggattgt gaccaatacc tccattcgca ttgctggaga tgagatggat
    12001 gaagccatcg tgttttattt aaaaaagaac tacaatcttc tgataggcga taggaccgct
    12061 gaagagataa aaataaaaat aggcacagcc tatccccagg acgaagaaga gagcatagag
    12121 ataaggggtc gggacttggt ggcgggaatt cccaagacta tgaagataag ctcagttcag
    12181 gtgagggagg cgctatccga gccggtgagc gccatcgtgg atgcggtcaa actcacactg
    12241 gaacaaactc cacctgagct ttccgccgat attttggatc gagggataat cttgacaggt
    12301 ggcggggctt tacttaaagg acttgaccag agattaagag aggaaaccag cttgccggtg
    12361 aatgtggcgg atgacccctt aacctgtgtt gctcgcggca ccggtaaggt tttagaggat
    12421 atgtcgcgtt atgccaaggt cttaatgagg ggaagaaaag aataaagtca ctcaaatttt
    12481 cgcaacttac attcgtatga tttgtataaa cgtataaatc ctaaacctta aatccaaaat
    12541 gaggaggtga aaattgaaga agcttaa
//
LOCUS       DASWLN010000153         2975 bp    DNA     linear   ENV 20-FEB-2024
DEFINITION  MAG TPA_asm: Candidatus Zixiibacteriota bacterium isolate
            SMAG_U2949 k141_1014054, whole genome shotgun sequence.
ACCESSION   DASWLN010000153 DASWLN010000000
VERSION     DASWLN010000153.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37960594
            Sequence Read Archive: SRR10376091
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Candidatus Zixiibacteriota bacterium (soil metagenome)
  ORGANISM  Candidatus Zixiibacteriota bacterium
            Bacteria.
REFERENCE   1  (bases 1 to 2975)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 2975)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (26-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/12/2024 08:28:26
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 1,440
            CDSs (total)                      :: 1,416
            Genes (coding)                    :: 1,412
            CDSs (with protein)               :: 1,412
            Genes (RNA)                       :: 24
            rRNAs                             :: 1 (16S)
            partial rRNAs                     :: 1 (16S)
            tRNAs                             :: 21
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 4
            CDSs (without protein)            :: 4
            Pseudo Genes (ambiguous residues) :: 0 of 4
            Pseudo Genes (frameshifted)       :: 0 of 4
            Pseudo Genes (incomplete)         :: 3 of 4
            Pseudo Genes (internal stop)      :: 1 of 4
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2975
                     /organism="Candidatus Zixiibacteriota bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_1014054"
                     /isolate="SMAG_U2949"
                     /isolation_source="soil"
                     /db_xref="taxon:2053527"
                     /environmental_sample
                     /country="USA: Oregon"
                     /lat_lon="42.99 N 123.63 W"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            <1..1530
                     /locus_tag="VGB16_05095"
     CDS             <1..1530
                     /locus_tag="VGB16_05095"
                     /inference="COORDINATES: protein motif:HMM:NF012869.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_function: GO:0042626 - ATPase-coupled transmembrane
                     transporter activity [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="ABC transporter ATP-binding protein"
                     /protein_id="HEX9917095.1"
                     /translation="DYIHSYLMAFVQQSVTRDLRAELYSHYQKLSLDYFQRTRTGQLM
                     SRITNDVLVLNDAIDITFARLIRDPLLVVIFLGFILIISWKLTLLAMVVLPLSLFAVT
                     RIGRYLKRYSTRTQERMADINSILEETVSGIRIVKGFAKSKFEIGKFKKALQRHFSAI
                     LKMTRVRLLSNPINEFLATVVGVIILWYGGRQVLIGELLKPDDFVLYIFAMFSLIQPI
                     KSLSQVYGKIQEGMAATQRIFEVLDTKPKVTSSPHGIKKVDFKDSLVFEHVHFEYNPG
                     DVVLKDIDLVIKAGEIVAFVGPSGAGKSTLLDLVPRFYDPTGGQIKLDGIDIKDIDVE
                     HLRGLLGIVTQETILFYDTIFNNVAYGMENVPENKVISAAKIANAHDFIINLREGYQT
                     YIGTRGFRLSGGERQRLAIARAVLRDPKILIFDEATSSLDTESEILVQEAIDRLMQDR
                     TTLVIAHRLSTVQHADKIVVLDKGEIVQQGRHSELVEKEGLYGKLYNLQFNYDDYNQK
                     S"
     gene            1475..2602
                     /locus_tag="VGB16_05100"
     CDS             1475..2602
                     /locus_tag="VGB16_05100"
                     /inference="COORDINATES: protein motif:HMM:NF013257.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="glycosyltransferase family 9 protein"
                     /protein_id="HEX9917096.1"
                     /translation="MANSIICNLTMTTITKKVEVASRKIFFGLIRWLLEREHKVSKEI
                     KPESIKKVLFLRYDKMGDMIISLPVFKALKEKYANMHIAVMCSPRNKVVLKGNKNVDE
                     IFVYRKRLFTDIKTVVEMRKRRFDCLVDLVFWESVTSAILTAVVRGSGISIGVGKGTF
                     KEFYDHIIPTERESKEHIIPKTMQVLEIFGIDPKSCDLYPHLSISEDDELQAKIFLEK
                     IKGSPIIGLNISAGQPNRLWSLEKFAKLVHLLKNENQKSSFVIIYSVKDKNRALQLQS
                     RLNSQAVLIPFESSFQRVAAIIQNLDLLITPDTSLVPVAWSSGVSMVGLYSADLENFH
                     FWRPLTPNSRTVMSSSYYDIFDIEPEEVFKAANSLLTERNK"
     gene            2658..>2975
                     /locus_tag="VGB16_05105"
     CDS             2658..>2975
                     /locus_tag="VGB16_05105"
                     /EC_number="2.4.-.-"
                     /inference="COORDINATES: protein motif:HMM:NF012745.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016757 - glycosyltransferase activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="glycosyltransferase family 2 protein"
                     /protein_id="HEX9917097.1"
                     /translation="MSREKITLSVIVIALDEEKNIERCLESLTWADELILVDSGSTDK
                     TKELASKYTDKMFDFKWDGFGKSKEYAKSKAKGVWVLSIDCDEVVSDKLKDEILTKIN
                     YPEG"
BASE COUNT          882 a          566 c          713 g          814 t
ORIGIN      
        1 gattacatac atagctacct gatggctttt gtgcagcaga gtgtcacccg cgatttgagg
       61 gcggagctgt attctcatta ccaaaagctt tctcttgact actttcagcg aacccgcacc
      121 gggcaactaa tgtccagaat aactaatgac gttttggtct taaacgacgc catagacatt
      181 acctttgccc gtttaattcg ggacccgctt ctggtagtaa tcttccttgg tttcatcttg
      241 attatcagct ggaagctaac acttttggct atggtggttc tgccgcttag tcttttcgcc
      301 gtaacccgta ttggaagata cctcaagaga tacagtacac gcactcagga gagaatggcg
      361 gacataaact ccattttgga ggagacggtt tccggaataa gaattgtcaa aggttttgcc
      421 aaaagcaagt ttgaaatcgg caaattcaaa aaagccctgc aaagacattt ttccgcaatc
      481 ttaaagatga cccgagtgag acttctttcc aatccgatca atgagttttt agcgacagtt
      541 gtaggggtca taatcctttg gtatggtgga aggcaggtct tgattggtga actcttgaag
      601 ccggatgatt tcgtcctcta tatcttcgct atgttctctt tgattcagcc cataaaaagt
      661 ttaagtcagg tttatggcaa aatacaggag gggatggcgg caacgcagag aatctttgag
      721 gttttggata ccaagcccaa agtgacaagc agtcctcatg ggattaagaa agttgacttt
      781 aaggacagtc tggtttttga acatgttcat tttgaatata atcccggaga tgttgttctg
      841 aaggatattg atctggtaat aaaagcagga gagattgtgg cttttgtggg tccctcgggt
      901 gcgggcaaat caactttatt ggatttggtg ccgcgctttt acgaccccac cggtggtcaa
      961 ataaagttgg atgggataga tataaaggat attgatgtgg aacacctcag gggactttta
     1021 ggcattgtga cgcaggagac aatccttttt tacgatacca tatttaataa cgttgcttat
     1081 ggtatggaga atgttccgga aaataaagtc atctccgcag ccaagatagc caatgcccac
     1141 gacttcatta taaacctgcg ggaagggtat cagacttaca tcggcaccag aggatttcgg
     1201 ctctccggcg gagaaaggca gagattggca attgcgcggg cagtcttaag agacccaaag
     1261 atattgattt tcgatgaagc cacatcctct ttggacaccg aatctgagat tctggtgcag
     1321 gaggcgatcg atcgcttaat gcaggatcgg accaccctgg ttatagctca tcgcctgtcc
     1381 acagtccagc atgcggataa gatcgttgtt ttagacaagg gggaaatcgt ccagcaaggc
     1441 agacattcag aacttgtgga aaaagagggg ctttatggca aactctataa tctgcaattt
     1501 aactatgacg actataacca aaaaagttga ggtagcaagt cgcaagatat tctttggact
     1561 tatccgttgg ctattggagc gggaacacaa ggtctccaaa gagattaaac ccgaaagcat
     1621 aaagaaggtc ttgtttttgc ggtatgataa gatgggagat atgattataa gcctgccggt
     1681 tttcaaagcc ctgaaagaga aatacgctaa catgcatata gcagtgatgt gcagtccaag
     1741 aaataaagtt gtcttgaaag gtaacaaaaa cgtagatgag atttttgtct atcgcaaaag
     1801 attatttact gacatcaaaa ccgtggtgga gatgaggaaa aggagattcg attgtttggt
     1861 cgatctggtt ttttgggaat cagtaacttc agctatcttg accgcagtgg ttaggggaag
     1921 cggcatctca atcggggttg gaaagggaac tttcaaggag ttctacgacc acattattcc
     1981 cactgaacgc gaaagcaagg agcatataat tccgaaaaca atgcaggttt tggagatctt
     2041 cgggatcgac ccaaaaagtt gtgatctata tccacatctg tcgatatcgg aagatgatga
     2101 gctacaagca aagatattcc ttgagaaaat aaaggggagt cccataatcg gtcttaatat
     2161 ttcagctgga cagccgaaca ggttgtggtc tttagaaaag ttcgccaagc tagtccatct
     2221 tttgaaaaac gaaaaccaaa aaagcagttt cgttatcatt tattcggtca aggataagaa
     2281 ccgagcactt cagcttcaat ctagattgaa tagccaggca gtgcttattc cgtttgaatc
     2341 gagctttcag cgggtagcag ccatcataca aaatttggat ctcttaatca ctccagacac
     2401 ttcgcttgtt cctgttgcct ggtcatctgg cgtttcaatg gtgggactct acagcgctga
     2461 tctggaaaac tttcatttct ggaggccttt gactcccaac agccgtacgg ttatgtccag
     2521 ctcctactac gatatctttg atatcgagcc cgaagaggta tttaaggcgg caaattcgct
     2581 tttaactgaa agaaacaagt aagcgattat taatttatcc tccggttgtt tcgtttatac
     2641 cataaaacta acagactatg agtagagaaa aaataacttt gagcgtaatc gttattgccc
     2701 tggatgaaga gaagaatatc gaaagatgct tggagagctt aacctgggca gatgagctaa
     2761 tcttagtcga tagcggaagt acagataaaa ctaaggagtt agcatcgaaa tacacggata
     2821 agatgtttga tttcaagtgg gacgggtttg gcaaatctaa ggaatacgct aaaagcaaag
     2881 ccaaaggtgt atgggtttta tccattgatt gcgatgaggt ggtttcggat aagctgaaag
     2941 atgaaatact aactaagata aattatccag agggc
//
LOCUS       DASWLN010000154         4825 bp    DNA     linear   ENV 20-FEB-2024
DEFINITION  MAG TPA_asm: Candidatus Zixiibacteriota bacterium isolate
            SMAG_U2949 k141_203652, whole genome shotgun sequence.
ACCESSION   DASWLN010000154 DASWLN010000000
VERSION     DASWLN010000154.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37960594
            Sequence Read Archive: SRR10376091
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Candidatus Zixiibacteriota bacterium (soil metagenome)
  ORGANISM  Candidatus Zixiibacteriota bacterium
            Bacteria.
REFERENCE   1  (bases 1 to 4825)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 4825)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (26-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/12/2024 08:28:26
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 1,440
            CDSs (total)                      :: 1,416
            Genes (coding)                    :: 1,412
            CDSs (with protein)               :: 1,412
            Genes (RNA)                       :: 24
            rRNAs                             :: 1 (16S)
            partial rRNAs                     :: 1 (16S)
            tRNAs                             :: 21
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 4
            CDSs (without protein)            :: 4
            Pseudo Genes (ambiguous residues) :: 0 of 4
            Pseudo Genes (frameshifted)       :: 0 of 4
            Pseudo Genes (incomplete)         :: 3 of 4
            Pseudo Genes (internal stop)      :: 1 of 4
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..4825
                     /organism="Candidatus Zixiibacteriota bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_203652"
                     /isolate="SMAG_U2949"
                     /isolation_source="soil"
                     /db_xref="taxon:2053527"
                     /environmental_sample
                     /country="USA: Oregon"
                     /lat_lon="42.99 N 123.63 W"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            <1..499
                     /locus_tag="VGB16_05110"
     CDS             <1..499
                     /locus_tag="VGB16_05110"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_014100182.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=2
                     /transl_table=11
                     /product="type II secretion system F family protein"
                     /protein_id="HEX9917098.1"
                     /translation="YFSRTPKGRLFFDKIKLRIPLIGDLIRKSAISRFSRTLGTLITS
                     GVPILDALDITARTSGNQVIHDAIKKSVLSIAEGETITQPLRDSGVFPPMVVQMIGVG
                     EKTGGLDEMLTKIGDFYDEEVDAAVSSLTSIIEPVIIVFMGVVIGGILIAMYLPMFEI
                     VTKIA"
     gene            562..2304
                     /locus_tag="VGB16_05115"
     CDS             562..2304
                     /locus_tag="VGB16_05115"
                     /inference="COORDINATES: protein motif:HMM:NF014567.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ATP-binding protein"
                     /protein_id="HEX9917099.1"
                     /translation="MEKNHIYSEEKTKQMFWFLAIRLAIFLILLVLVVFLLGAPEHLL
                     FPFIIYSLCTLFCLSIILFERTVPSEGLFKSVAGIQVILEALIEGGIIYATREINSPF
                     AILFVLTIVSSSLLYELVGSLLIATMVSLTYTVTLLVTAGVEFSFLLDPKNLIALYQG
                     VTDAFYYRIFLYICTFYLVAFISGYLTQKLKAKGKELISASEKLSQIRTDTDDILQHM
                     RSGLVTIDSAGKIIYFNRTAEDILGFLSAEIKGRDYLEVFVESVPEFADKLNQILKSR
                     RAELRSEIWITSRNGKKIPLGISTSILGDKDTGGRGVIAVFQDISEAKKLEERVRIAD
                     RLAAVGELSAGIAHEIRNPLASISGSVEVLKDELALEGENGKLMELIVKETARLNHIL
                     TEFLQYAKIKQTPLKKVEMGHLIDDILEVVKKHPSYVPGINLEKEMDEGPIYILGDEN
                     QIKQLLLNLIVNALEAIEEEKGEVIIANRSLDQIEGYYFEGEEDLDNLGWVPLAVIDR
                     GKGMTQEQLEKVFQPFYSTKKNGTGLGLAIVQRLVNNLNGRIEFKSQLGIGSVFVVYF
                     QKYETHKEELQVTF"
     gene            2633..2875
                     /locus_tag="VGB16_05120"
     CDS             2633..2875
                     /locus_tag="VGB16_05120"
                     /inference="COORDINATES: protein motif:HMM:NF021073.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="AtpZ/AtpI family protein"
                     /protein_id="HEX9917100.1"
                     /translation="MSKEAPDKYWWVKQASAFTTIPVILLVGPLVGYLIGSFLDRRLG
                     TQPYLMLVLVVLGFAASGKEIYRMIKKFSPKDLDGN"
     gene            2865..3260
                     /locus_tag="VGB16_05125"
     CDS             2865..3260
                     /locus_tag="VGB16_05125"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEX9917101.1"
                     /translation="MGIEFIHRTIRTSIVLACAIFLSVWLYYDFKFGAGILVGALWGC
                     LNLFAIALVVKVAITSQIAKKKIILSVLFFKFPFIYGIGYIILKLDFFPILSLLAGFT
                     SIFLVMFMKALGQMLMSVRRENQLGSVTK"
     gene            3274..4101
                     /gene="atpB"
                     /locus_tag="VGB16_05130"
     CDS             3274..4101
                     /gene="atpB"
                     /locus_tag="VGB16_05130"
                     /EC_number="7.1.2.2"
                     /inference="COORDINATES: protein motif:HMM:TIGR01131.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0000276 - mitochondrial
                     proton-transporting ATP synthase complex, coupling factor
                     F(o) [Evidence IEA];
                     GO_component: GO:0045263 - proton-transporting ATP
                     synthase complex, coupling factor F(o) [Evidence IEA];
                     GO_component: GO:0045264 - plasma membrane
                     proton-transporting ATP synthase complex, coupling factor
                     F(o) [Evidence IEA];
                     GO_function: GO:0046933 - proton-transporting ATP synthase
                     activity, rotational mechanism [Evidence IEA];
                     GO_process: GO:0015986 - proton motive force-driven ATP
                     synthesis [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="F0F1 ATP synthase subunit A"
                     /protein_id="HEX9917102.1"
                     /translation="MFSFALISKTAEAVRQVSCQTETSNTPELPNIVQIVSSLLHNSG
                     IAHFLEYYQNLFFSIIVIIFLGILFAKASGKAKLVPGKLQNFLEIPIEWLHDLVIGVL
                     GKRGEKYLPFVGTIFIYILGMNLLGLVPTMKSPTSSLNTTLALALTVFIYVQYTGIRN
                     LGLLGYLDHLAGEPRGAVGFAMIPLMLPLHIMGELVRPVSLSLRLFGNILGEDSLIGA
                     LASLGVVALSFLHFPYVGIPFQLPFVILAILFSTIQALVFTLLSTIYISLMLPHEEH"
     gene            4130..4381
                     /gene="atpE"
                     /locus_tag="VGB16_05135"
     CDS             4130..4381
                     /gene="atpE"
                     /locus_tag="VGB16_05135"
                     /EC_number="7.1.2.2"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011176219.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0000276 - mitochondrial
                     proton-transporting ATP synthase complex, coupling factor
                     F(o) [Evidence IEA];
                     GO_component: GO:0045263 - proton-transporting ATP
                     synthase complex, coupling factor F(o) [Evidence IEA];
                     GO_component: GO:0045264 - plasma membrane
                     proton-transporting ATP synthase complex, coupling factor
                     F(o) [Evidence IEA];
                     GO_function: GO:0046933 - proton-transporting ATP synthase
                     activity, rotational mechanism [Evidence IEA];
                     GO_process: GO:0015986 - proton motive force-driven ATP
                     synthesis [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="ATP synthase F0 subunit C"
                     /protein_id="HEX9917103.1"
                     /translation="MNLYLYALTLPFGAIAVGIAAVGSAYGLGRAVSAAMEAIGRQPE
                     AAGKIQLAMIIGAAFIEALTIYALLAVILLMTKFPAIKI"
     gene            4399..>4825
                     /gene="atpF"
                     /locus_tag="VGB16_05140"
     CDS             4399..>4825
                     /gene="atpF"
                     /locus_tag="VGB16_05140"
                     /EC_number="7.1.2.2"
                     /inference="COORDINATES: protein motif:HMM:NF012645.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0000276 - mitochondrial
                     proton-transporting ATP synthase complex, coupling factor
                     F(o) [Evidence IEA];
                     GO_component: GO:0045263 - proton-transporting ATP
                     synthase complex, coupling factor F(o) [Evidence IEA];
                     GO_component: GO:0045264 - plasma membrane
                     proton-transporting ATP synthase complex, coupling factor
                     F(o) [Evidence IEA];
                     GO_function: GO:0046933 - proton-transporting ATP synthase
                     activity, rotational mechanism [Evidence IEA];
                     GO_process: GO:0015986 - proton motive force-driven ATP
                     synthesis [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="F0F1 ATP synthase subunit B"
                     /protein_id="HEX9917104.1"
                     /translation="MEEIVGALGIKWGELFAQVVAFLVVLWILKKWAWKPILKILEDR
                     RQKIQSEFDGISQKKKEVETLVSDYQAKLENIDSLSRAKIQEAASEGQKLANEIRENA
                     RKDAKELVDRSKEEIQRDIEKAKVQLRDDLVKISLGAAEK"
BASE COUNT         1339 a          910 c         1161 g         1415 t
ORIGIN      
        1 gtacttttcc aggaccccca agggccgatt attctttgat aagataaagt taagaattcc
       61 cttgattggg gacctcatta gaaaaagtgc tatctcgcgc ttttccagaa ccctggggac
      121 attaatcacc tctggcgttc ccattctgga tgcgctggat ataactgcac gcacctcggg
      181 caatcaagtc attcacgatg ccataaagaa atcggttctt tcgatcgccg aaggagagac
      241 tatcactcag ccgctgaggg attccggtgt atttccgccc atggtggttc aaatgatagg
      301 agtgggagag aagacgggtg ggctggatga gatgctgaca aagatcggag acttttatga
      361 cgaggaagtg gatgctgcag tatcatcctt aacttctatt attgagccgg tcatcatagt
      421 ttttatggga gtagtgatag gtggaattct gattgcaatg tatctgccta tgtttgagat
      481 agtaactaag atagcttaag gatcattcac cgaaaggcaa gctaaccgct gtcccaaaag
      541 gcagtggtta gctttttcgc tatggaaaag aatcacatat acagcgaaga aaagaccaag
      601 cagatgtttt ggttcttggc aatcagactt gccatctttc tcatcctttt ggtcctagtg
      661 gtgtttttgc taggcgcacc cgaacatctt ttgtttccat tcatcatcta cagtttatgt
      721 accctctttt gtctttccat catactattc gagaggacgg ttccttcaga gggcttattc
      781 aagtcggttg caggtataca ggttatcttg gaggcgctca tcgaaggagg gataatttat
      841 gctaccaggg agataaatag tccttttgcg attctttttg ttctgaccat tgtctcatcc
      901 agcttattgt atgagctggt ggggagcctt ttaatagcta ctatggtcag tttgacttac
      961 acggtaactc ttctggttac ggctggagtc gagttttcct tcctactcga ccccaaaaat
     1021 ctgatagctc tttaccaagg agtcacagac gccttctatt acaggatttt tctttacatc
     1081 tgcactttct atctggtggc ttttatcagc gggtatttaa cccagaaact gaaggccaag
     1141 ggaaaagaac ttatctccgc ttccgagaag cttagtcaaa taaggaccga cacagacgac
     1201 attttacagc atatgcgtag cggtctggtg actatcgact cggctggcaa aattatatat
     1261 tttaacagga ccgcagaaga tatactgggc tttttatctg cagagataaa aggcagagac
     1321 tacctggagg tatttgtaga gagcgtaccg gaatttgcag acaagttaaa ccagatcttg
     1381 aaatctcgtc gagctgaatt gcgcagtgag atctggatta cttctcgcaa cggcaagaaa
     1441 atacctctgg gcataagcac ctcgattctg ggtgataaag ataccggagg aaggggagtc
     1501 atcgctgttt ttcaagacat atctgaagcc aagaagctgg aagaaagagt acgcatcgca
     1561 gatagattag cggcagtggg agagctttcc gccgggatag cacatgagat tagaaatccg
     1621 ttagcttcaa tctccggctc agttgaagtg ctgaaagacg aattagcttt ggagggggag
     1681 aatggcaagc tcatggaatt gatagtcaag gaaactgcca gactgaatca tattttaact
     1741 gagtttttgc agtatgccaa aataaaacag actcccttga aaaaagttga gatgggtcat
     1801 ctaatagatg atatcctgga ggtggtgaaa aagcacccat cctatgttcc cgggattaat
     1861 ttggagaagg agatggatga ggggccgatt tacattttgg gtgatgagaa tcagataaaa
     1921 cagctcctct tgaatttgat agttaatgct ttggaggcga tagaggaaga aaagggagaa
     1981 gtcataattg ctaatcgcag tctggaccag atagaaggtt attacttcga gggggaagaa
     2041 gatttagata acttggggtg ggttcctttg gctgtaatcg acagagggaa gggtatgact
     2101 caggaacaac tggaaaaggt attccagcca ttttactcca ccaagaaaaa cggcaccggt
     2161 ctggggctgg ctattgttca gcgtttggtc aacaacttaa acggaagaat agaatttaaa
     2221 agccagttgg gaatagggtc tgtgtttgtg gtttacttcc agaaatatga gacccataaa
     2281 gaagaattgc aggttacctt ctaatctaag gttcaaactc tcgctcaaaa aaagagttcc
     2341 tcctaacatg cagatcggct cagccaaaaa agaccaccga aaaagtggtc tttgtagaat
     2401 ttttggagtg tcccgctttt agcgggacca aaatgaatta tcttctgtgg tgagtttatc
     2461 gaaccattgt tttttaatag gttaaaagta aagctaaagc tttacactcc aatggcgctc
     2521 tttagaaaat tgactttttc agaccaacaa aaataacttg acaacccaag ttcgtggcgt
     2581 ataataataa gttatctctc aaattatatt aactcagata attcctattc aattgtccaa
     2641 agaagcaccc gataaatatt ggtgggtaaa gcaggcgagc gcattcacaa ccatacccgt
     2701 gattttgctg gttggtccat tggtcgggta tctcataggt agttttctag ataggagatt
     2761 aggtactcag ccgtatttaa tgttagttct tgtggtcttg ggttttgcgg cgagcggtaa
     2821 ggagatttat cgcatgatca aaaagtttag cccaaaggat ttagatggga attgagttta
     2881 tccacaggac gattcgcact tcaatagttc tagcttgcgc cattttctta tcagtttggt
     2941 tgtattatga tttcaagttt ggagccggga tactggtagg tgctctctgg ggatgtttga
     3001 atctctttgc cattgccctg gtagtcaagg tggcgataac ttcccagata gctaagaaga
     3061 aaattatcct ctcggttctc ttcttcaaat ttcctttcat ttatggcatt gggtacatta
     3121 ttttgaaact cgatttcttt ccaatcttga gtctacttgc gggtttcact tcgattttct
     3181 tagttatgtt tatgaaagct ttgggacaga tgctgatgtc agtcagacga gaaaaccagt
     3241 tagggagtgt gacaaagtaa ggggaaatta aatatgttct catttgctct aatctcaaag
     3301 acagcggaag cagtaagaca ggtgagttgc cagacagaaa ccagcaacac tccagagtta
     3361 cctaacattg ttcaaatcgt atcttcgctg ttacataata gcggaatcgc tcacttttta
     3421 gaatactacc agaatttgtt tttttcaatt attgtaatca tattcttggg aatcttattc
     3481 gccaaggcgt ctggcaaggc taagctcgtt ccaggcaaat tacagaactt cctggaaata
     3541 ccgattgagt ggctccatga tctggttatt ggcgtcttag gtaaaagagg agaaaaatat
     3601 ctgccatttg tgggaacgat attcatttac atattaggta tgaacttatt gggcttggtg
     3661 ccaacaatga aatccccgac ctcaagttta aacactacct tagctctggc tttgacggta
     3721 ttcatatacg ttcaatacac cggcataaga aacctgggac ttttgggtta tctggaccat
     3781 ttagctggtg agccgagagg ggcagttggt tttgctatga tacctttgat gttgccgctt
     3841 cacattatgg gggagcttgt caggccggtt agcttatcct tgcgtttgtt tggaaacatc
     3901 ctgggcgaag attctctcat cggcgccttg gcttccttag gcgtagtagc tttgagtttc
     3961 ttgcattttc cctatgtggg cataccgttt cagttgcctt ttgtgatatt ggcgattctt
     4021 tttagtacta ttcaagcatt agtttttaca ttactttcaa ccatttatat atctttgatg
     4081 ttaccacatg aagaacattg aagtaacctt aaaataacag gaggggtttg tgaaccttta
     4141 tctttatgct ttgacgctac cttttggggc gatcgcggtt ggcatagccg cagttggttc
     4201 agcttacggt ttaggtcggg cagtatcagc cgcgatggaa gccatcggaa gacagccaga
     4261 ggcggctggg aagatacagc ttgctatgat cattggcgca gcattcattg aggctttgac
     4321 catttatgct cttttggctg tgattctttt aatgaccaag ttcccagcaa ttaagattta
     4381 acctttagag agattgatat ggaagagatc gtaggcgctt tgggtattaa gtggggtgaa
     4441 ctttttgccc aagttgtggc ttttttggtc gttttgtgga ttttgaagaa gtgggcttgg
     4501 aagcctatct taaaaatttt agaggatagg cgtcaaaaaa tacaatctga attcgatggc
     4561 atctcccaga agaaaaaaga ggtggaaacc ctggtctcgg attaccaagc caagctggag
     4621 aatatcgatt ccttaagccg agccaaaatc caggaggcag caagtgaggg tcagaaatta
     4681 gctaacgaga taagggaaaa tgcgcgcaaa gatgccaagg agcttgttga tcgctcgaaa
     4741 gaggaaatac aaagagacat cgagaaagcc aaggtccaac tgcgagacga tttagttaaa
     4801 atcagcctgg gtgcggcgga gaaga
//
LOCUS       DASWLN010000155         1970 bp    DNA     linear   ENV 20-FEB-2024
DEFINITION  MAG TPA_asm: Candidatus Zixiibacteriota bacterium isolate
            SMAG_U2949 k141_203770, whole genome shotgun sequence.
ACCESSION   DASWLN010000155 DASWLN010000000
VERSION     DASWLN010000155.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37960594
            Sequence Read Archive: SRR10376091
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Candidatus Zixiibacteriota bacterium (soil metagenome)
  ORGANISM  Candidatus Zixiibacteriota bacterium
            Bacteria.
REFERENCE   1  (bases 1 to 1970)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 1970)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (26-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/12/2024 08:28:26
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 1,440
            CDSs (total)                      :: 1,416
            Genes (coding)                    :: 1,412
            CDSs (with protein)               :: 1,412
            Genes (RNA)                       :: 24
            rRNAs                             :: 1 (16S)
            partial rRNAs                     :: 1 (16S)
            tRNAs                             :: 21
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 4
            CDSs (without protein)            :: 4
            Pseudo Genes (ambiguous residues) :: 0 of 4
            Pseudo Genes (frameshifted)       :: 0 of 4
            Pseudo Genes (incomplete)         :: 3 of 4
            Pseudo Genes (internal stop)      :: 1 of 4
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..1970
                     /organism="Candidatus Zixiibacteriota bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_203770"
                     /isolate="SMAG_U2949"
                     /isolation_source="soil"
                     /db_xref="taxon:2053527"
                     /environmental_sample
                     /country="USA: Oregon"
                     /lat_lon="42.99 N 123.63 W"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            <1..1949
                     /gene="dnaE"
                     /locus_tag="VGB16_05145"
     CDS             <1..1949
                     /gene="dnaE"
                     /locus_tag="VGB16_05145"
                     /EC_number="2.7.7.7"
                     /inference="COORDINATES: protein motif:HMM:TIGR00594.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0009360 - DNA polymerase III complex
                     [Evidence IEA];
                     GO_function: GO:0003887 - DNA-directed DNA polymerase
                     activity [Evidence IEA];
                     GO_process: GO:0006260 - DNA replication [Evidence IEA]"
                     /codon_start=3
                     /transl_table=11
                     /product="DNA polymerase III subunit alpha"
                     /protein_id="HEX9917105.1"
                     /translation="KTLEGLSRHASTHAAGVVIAPGDLTEFVPLYLSNNDEVTTQYDM
                     KGIEEIGLLKIDFLGLRTLTVIDDTLSMVREQKGVNIDMDSIPLDDKKVFELFSKGET
                     IGVFQFESSGMRDYLKKLKPESIEDLTAMNALYRPGPLDSHMIDEYIDRKWGRKKIKY
                     EHAMLEPILRETYGVIVYQEQVLKVASDLAGYSLGKADILRKAMGKKQSEMMAEQREE
                     FIKGAKNNNIDARTADRIFDFMATFARYGFNKSHSVGYAHIAYQTAYFKAHYPVEFMA
                     ALLSSEKDDSTRIVTLMDECRRMGITVLPPDVNESLCAFSMVGDKIRFGLGAVKNVGE
                     SAVMAIIKAREKYGRFNTIFEFCERVDSRALNKRTIESLIQAGASDSLKGHRAQLMGC
                     ADMAITYGQAVQQDKMRGQTSLFGGSIDATALSQPKLLDIPKWPTSEVLSREKEMLGF
                     YVSGHPLAKYDEELKLFTTKDSETLQSTRDGEEVVIGGVITNVKFNLDKKGKRMAFAT
                     VEDFGGMTEVVIFSDCLEKNKDTVRTESIVVVKGRASTKESERAKIVAFEVVNLENAY
                     RNDKLILHIILNTEQSDQNFILGLKEMLSSQPGGAGVVLHLSSNGGEVKVKLKNLSIG
                     LSKELLENLKKKVGEKKVYLDKKA"
BASE COUNT          608 a          352 c          514 g          496 t
ORIGIN      
        1 ctaaaaccct ggaaggttta tctcggcatg cctccacgca tgcggcagga gtggtgattg
       61 ctcctgggga tttgaccgaa ttcgtccctt tgtatctgag caacaacgac gaagtaacca
      121 cacagtatga tatgaaagga attgaagaga tcgggctttt gaaaatagat tttttgggtt
      181 tgagaacttt gacggtaatt gatgatactc tttcgatggt aagggaacaa aaaggagtga
      241 atatagatat ggattcaatt cctttagacg acaagaaggt tttcgagctt ttctcaaaag
      301 gggagaccat tggcgtgttt cagtttgaaa gctcgggaat gagagattat ctgaaaaagt
      361 tgaaaccgga gagcatagag gatttgacag cgatgaatgc tctctaccgt cccggaccct
      421 tggatagtca tatgatcgac gaatatatcg accgcaagtg gggtcgaaag aaaataaaat
      481 acgaacatgc gatgcttgag ccaatcttaa gggagacata tggagttatc gtttatcagg
      541 agcaggtttt aaaggtcgcc agtgatttgg ccggttacag tctgggcaag gcagatatcc
      601 taagaaaagc gatggggaaa aaacaaagcg aaatgatggc agagcagaga gaggaattca
      661 tcaaaggagc caagaacaac aatatcgatg cccgaaccgc tgacaggatt ttcgatttca
      721 tggcaacttt tgcccgttat ggattcaaca aatcgcacag cgtaggttac gctcatatag
      781 cttatcagac agcttatttt aaagcacatt atccagttga attcatggct gccttattgt
      841 cctctgaaaa ggatgattcc acgcgcatcg ttactctaat ggatgagtgt cggcgtatgg
      901 gaatcactgt tctccccccg gatgtgaatg agagcttatg cgctttcagt atggttggag
      961 ataagattcg ctttggattg ggtgcagtca aaaatgtagg tgaatcagcg gtaatggcta
     1021 taatcaaagc cagagagaaa tatggcaggt ttaacaccat atttgaattc tgcgaaagag
     1081 tggactcaag agctttaaac aagagaacca tagagagtct aattcaggcg ggagcttctg
     1141 actccctgaa gggtcatagg gcacaactta tgggttgtgc ggatatggct ataacctatg
     1201 gacaagctgt acagcaggat aaaatgcgtg gccagacatc gctttttggc ggaagcatag
     1261 atgctactgc tctatctcaa cctaaactgc tggacatacc caagtggccc accagcgaag
     1321 ttctttcacg agaaaaagag atgttaggtt tctatgtttc cggtcatccc ttggccaagt
     1381 atgatgagga attgaagctt ttcaccacta aagacagtga gactcttcaa tccacacgag
     1441 atggggagga ggtagtaatt ggtggggtta tcaccaatgt gaaattcaac ctggataaga
     1501 aagggaaacg catggctttt gccacggtgg aggattttgg gggtatgacc gaggtggtaa
     1561 ttttttccga ctgtttggaa aaaaacaagg acacagttag gaccgagtcg atagttgtag
     1621 taaaagggag agcttccacc aaggagagcg aaagagccaa aatagtggct ttcgaggtcg
     1681 tcaatttaga gaatgcctac cgcaatgata aattgatctt gcacataatc ctgaacacag
     1741 aacaaagcga ccagaatttc attttggggc tgaaggagat gctttcttcc cagccgggcg
     1801 gagcaggggt ggtcttacat ttgagttcaa atggcggtga agttaaggtg aaattgaaga
     1861 atctctcgat aggactttcc aaggaactct tagaaaatct gaagaaaaaa gtgggagaga
     1921 aaaaggtcta tttggataaa aaagcttgag gatgtaattc tatagcaaac
//
LOCUS       DASWLN010000156         3207 bp    DNA     linear   ENV 20-FEB-2024
DEFINITION  MAG TPA_asm: Candidatus Zixiibacteriota bacterium isolate
            SMAG_U2949 k141_829873, whole genome shotgun sequence.
ACCESSION   DASWLN010000156 DASWLN010000000
VERSION     DASWLN010000156.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37960594
            Sequence Read Archive: SRR10376091
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Candidatus Zixiibacteriota bacterium (soil metagenome)
  ORGANISM  Candidatus Zixiibacteriota bacterium
            Bacteria.
REFERENCE   1  (bases 1 to 3207)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 3207)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (26-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/12/2024 08:28:26
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 1,440
            CDSs (total)                      :: 1,416
            Genes (coding)                    :: 1,412
            CDSs (with protein)               :: 1,412
            Genes (RNA)                       :: 24
            rRNAs                             :: 1 (16S)
            partial rRNAs                     :: 1 (16S)
            tRNAs                             :: 21
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 4
            CDSs (without protein)            :: 4
            Pseudo Genes (ambiguous residues) :: 0 of 4
            Pseudo Genes (frameshifted)       :: 0 of 4
            Pseudo Genes (incomplete)         :: 3 of 4
            Pseudo Genes (internal stop)      :: 1 of 4
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..3207
                     /organism="Candidatus Zixiibacteriota bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_829873"
                     /isolate="SMAG_U2949"
                     /isolation_source="soil"
                     /db_xref="taxon:2053527"
                     /environmental_sample
                     /country="USA: Oregon"
                     /lat_lon="42.99 N 123.63 W"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            <1..862
                     /locus_tag="VGB16_05150"
     CDS             <1..862
                     /locus_tag="VGB16_05150"
                     /inference="COORDINATES: protein motif:HMM:NF024753.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=2
                     /transl_table=11
                     /product="PQQ-binding-like beta-propeller repeat protein"
                     /protein_id="HEX9917106.1"
                     /translation="HLVDAQSGQKIGQYSFSSSITSTACVEGEMLYFGTEIDDNRILA
                     TSLKDGKTIWEKKTNDLCSSPLVWNGKIFFGTSSGYVSALNSLSGEVIWQFKTEGKIK
                     SSPMICDSVLYIGSLDNWFYALDAESGDLKWKYKSGAGVFSSAVAYDTLVYFGCADGY
                     LYALSRNTGDLVWKFKTGAAIYSSPTAKDGFVYFGSNDYCMYALNALSGELIWKFETG
                     GLVHSSPSVIGDKILFGSFDHNCYMLNRNNGELLWKYKTESMISSSPAYYQGKIYIAS
                     QDNYLYCFGK"
     gene            859..2976
                     /locus_tag="VGB16_05155"
     CDS             859..2976
                     /locus_tag="VGB16_05155"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+.
                     GO_function: GO:0005515 - protein binding [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="HEAT repeat domain-containing protein"
                     /protein_id="HEX9917107.1"
                     /translation="MISTTTHIDTTLTPDQLKTDLRQLGLGIVREMYLASQKLLAYSI
                     SHPAREKAVNSAFLHLRNWLQFKFYYQIETDGRALWTEKIRLEDNYFVRSFVSLLRKH
                     HLEEVKFYDWITPVELSYFIDSLSHFQKDPLSRLLQHNITSIEINPLIQRSSRFVAEP
                     RGDGYTVESLAQEELKGNPSTIIKIALDRFDQGEVVTNFRKEVLCSVAGKVFAGLSPS
                     LTCQLIEQELKANGSLAKFEKLIQLANEHPQRELLLTELDRSLRRAKLPSQDYATYFE
                     TYCSYPPGKSNEYLLKDQIIIESEDDFLSFVSDLLKAGEYSKLRLILDGLIEQLTSKS
                     ITTRSKSLSLVKSATPMLMTDQTLVSYLNGSLIEVWDEQKQTFEILDALCFVAETSLI
                     HQLYSDFRLILDFLKGKTKEEASPYKQEVFRKALVRLTGTEVVNKLIGDMNKSDGGEL
                     FEIGECLKCINTKEAAERLSRMINHPHPARRVMSLGILAEMENGGKTFVSQLLADEEI
                     ASELEKERIGPKNRVRIFEIIEVLSRAKKKEGIELLRKLCQFRDAEVRLKIASGLKGS
                     YNPEALILLRGLAVDLEVQVRLEAIRSLKNKVSSRDLEWLKAIYFEEDKNLEELICVI
                     ANLKFEDGDRFLFQALTQDKRLRKHPHCQIRILEVLSKRGRIVSKEQTRKLKYEWERK
                     SLFPFKKLILRRRVNQLILELYE"
BASE COUNT          981 a          580 c          737 g          909 t
ORIGIN      
        1 ccatttggtt gatgctcaga gtggacaaaa aataggccaa tatagctttt ccagtagcat
       61 tacttccacg gcttgcgtgg aaggagaaat gttatatttc gggaccgaga tagatgacaa
      121 tagaattctg gcgaccagtc ttaaagatgg aaaaactatc tgggaaaaga aaacaaatga
      181 cctttgttct tctcctttag tctggaatgg aaagatattt ttcggcacaa gcagtggcta
      241 cgtttctgca ctgaacagtt tatccggtga ggtgatctgg cagttcaaga ccgagggtaa
      301 gataaaatcc tcacccatga tttgtgatag cgttctctat attgggtcat tggacaattg
      361 gttttacgct ttagatgctg aaagcggaga tttgaaatgg aaatataagt ccggagcagg
      421 ggttttttcc tccgccgtgg cttatgacac cctggtttat tttggctgcg cagatgggta
      481 tctttatgct ttgagtcgca ataccggaga tttagtctgg aagttcaaga ccggagcagc
      541 gatttattca tctcctacag ctaaggatgg gtttgtttat ttcggctcta atgattattg
      601 tatgtatgct ttgaacgctt tgtcaggtga gcttatatgg aaattcgaaa cagggggact
      661 agttcattcc tctccatcgg tcattggaga caaaatcctg tttggctctt ttgaccataa
      721 ctgttatatg ctgaatagaa ataatggaga gctgttgtgg aaatataaga cagagagtat
      781 gatttcttcc tcacctgctt attatcaagg gaaaatatat attgcttcgc aagataacta
      841 tctatattgt tttggaaaat gatttcaact acaacccata ttgataccac tctaactccg
      901 gaccagctta agaccgacct gagacagctg ggcctgggta tcgtccgaga aatgtatctg
      961 gcttcccaga agctcttggc ttattccatt tctcatcctg ctagggaaaa agcggtcaat
     1021 tcagcttttc tccatctaag gaattggctg cagttcaaat tctattacca gattgagacg
     1081 gacggaaggg ctctatggac ggaaaagata aggttggaag ataactattt cgtcaggagt
     1141 ttcgtctccc tcttacggaa gcatcatctt gaagaggtta aattctacga ttggataaca
     1201 cccgttgagc tatcctattt tattgatagc ttgagtcatt ttcagaaaga tccactctca
     1261 cgtctacttc aacataacat aacttcaatt gagataaatc ccctgattca aagaagctcc
     1321 cgttttgtgg ctgaaccacg aggggatggg tatacggtgg aaagtctagc ccaagaggag
     1381 cttaagggaa acccatctac gataattaag atagctctcg atagatttga ccaaggagaa
     1441 gtggtgacaa acttccgcaa ggaggtttta tgttcagtcg caggaaaggt ttttgctggc
     1501 ctttctccga gtttaacatg tcaattgatt gagcaggagc tcaaagcaaa cggttcttta
     1561 gccaaatttg aaaagctcat acaacttgct aatgaacatc ctcaaaggga attgttactc
     1621 actgagctag acagatctct caggagagcg aaattaccat cacaggatta tgcaacctat
     1681 tttgaaacat attgctctta ccctccggga aaatcgaatg aatatttatt gaaagaccaa
     1741 ataatcatag aatcggaaga tgattttctg tcatttgttt ctgacctatt gaaggcagga
     1801 gagtatagca aactcagatt gatactggac ggtttgattg aacaactaac cagcaagagc
     1861 atcaccacca gaagtaaatc cttgagttta gtcaaatccg ccacaccaat gctcatgact
     1921 gaccagacgt tggtcagtta tctgaatggt agtttaattg aagtttggga tgaacaaaaa
     1981 cagaccttcg agatattgga tgccctctgt tttgtggcag aaacttcgct tatccatcaa
     2041 ctctattcag acttcagact gatattggat ttcctgaaag gaaaaaccaa agaggaagct
     2101 tcaccctata aacaagaggt tttccgaaaa gctctggtca ggttgacagg cactgaagtt
     2161 gtcaacaagc tgataggcga tatgaataag tccgatggag gtgagctgtt cgaaattgga
     2221 gagtgtttga aatgcatcaa caccaaggaa gcggcagaaa gattgagtcg aatgataaat
     2281 catcctcatc ctgccagaag ggtgatgtcg cttgggattt tggctgagat ggaaaacgga
     2341 gggaaaactt tcgtttcgca actcttggct gacgaggaaa tcgcaagtga gctcgagaag
     2401 gaaaggattg gtcctaaaaa tagggtgaga atttttgaaa taattgaggt tttgagcaga
     2461 gccaagaaaa aggaggggat tgaactgcta agaaagctgt gccagttcag agacgcggaa
     2521 gtgaggttaa aaattgcttc cgggcttaaa ggttcttata accccgaagc cctaatttta
     2581 ctgcgtggtc tggcagttga cctggaagtg caggtgagat tggaagctat tcgttcgttg
     2641 aagaataaag tttcatctcg tgacctggag tggctcaagg ctatttattt tgaggaggat
     2701 aaaaatctcg aagagttgat ttgtgttata gctaatctca agtttgaaga tggggataga
     2761 tttttgtttc aagccttgac gcaagataag agactacgaa aacatccaca ttgccagatt
     2821 cgcatattgg aagttttgtc caaaagaggc aggatagtgt caaaggagca aacccggaag
     2881 ctcaaatacg aatgggagag aaaatcgctt tttcctttca agaagcttat cttgagaaga
     2941 agagttaacc agctcatcct tgagttgtat gaatagactt aggcaagact cgagtctata
     3001 ttttccttga ctctttctgc gatttgaatt accattataa tagttaaaat caaacgtaac
     3061 caaaataata tacacattat aatagcatat taagatgtcc gctgtgtcaa acaataattc
     3121 aaagaatgcg ataggaaaga atgtaaggaa atttaggcaa gaaaaaggca tttctcaaga
     3181 tcggctttca aaacttgctg atttgtc
//
LOCUS       DASWLN010000157         6988 bp    DNA     linear   ENV 20-FEB-2024
DEFINITION  MAG TPA_asm: Candidatus Zixiibacteriota bacterium isolate
            SMAG_U2949 k141_205023, whole genome shotgun sequence.
ACCESSION   DASWLN010000157 DASWLN010000000
VERSION     DASWLN010000157.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37960594
            Sequence Read Archive: SRR10376091
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Candidatus Zixiibacteriota bacterium (soil metagenome)
  ORGANISM  Candidatus Zixiibacteriota bacterium
            Bacteria.
REFERENCE   1  (bases 1 to 6988)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 6988)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (26-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/12/2024 08:28:26
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 1,440
            CDSs (total)                      :: 1,416
            Genes (coding)                    :: 1,412
            CDSs (with protein)               :: 1,412
            Genes (RNA)                       :: 24
            rRNAs                             :: 1 (16S)
            partial rRNAs                     :: 1 (16S)
            tRNAs                             :: 21
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 4
            CDSs (without protein)            :: 4
            Pseudo Genes (ambiguous residues) :: 0 of 4
            Pseudo Genes (frameshifted)       :: 0 of 4
            Pseudo Genes (incomplete)         :: 3 of 4
            Pseudo Genes (internal stop)      :: 1 of 4
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..6988
                     /organism="Candidatus Zixiibacteriota bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_205023"
                     /isolate="SMAG_U2949"
                     /isolation_source="soil"
                     /db_xref="taxon:2053527"
                     /environmental_sample
                     /country="USA: Oregon"
                     /lat_lon="42.99 N 123.63 W"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            <1..332
                     /locus_tag="VGB16_05160"
     CDS             <1..332
                     /locus_tag="VGB16_05160"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=3
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEX9917108.1"
                     /translation="LIHELWENYWRQRKNNRLGEYDAHERAITEAENIVNNSVRLSRW
                     PYERPVISTTTRYGTVYSMKAYRRLIYEQGGKLYDVYINLCIPEASNIISVDVIERSS
                     STTQTEK"
     gene            348..803
                     /locus_tag="VGB16_05165"
     CDS             348..803
                     /locus_tag="VGB16_05165"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEX9917109.1"
                     /translation="MGRVLIFFSIFILLLGCSKYYRHGDRYSHTILIEGIFEASLGCV
                     EGYLTTPQNIVVTMNKGSDYLCEVKESISFLENVTGIQTSMTYGITEEKLSEENSSFL
                     AKDLEKWKRWYQDNKEDIFLSKDRKKLMYRKEDVKRIVDLSATRCWYPF"
     gene            919..1548
                     /locus_tag="VGB16_05170"
     CDS             919..1548
                     /locus_tag="VGB16_05170"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEX9917110.1"
                     /translation="MIGNHINKPLLLSIGITLVLFSFLSSRADELKVQDQGQLKSSEE
                     AKLPDTSSVPPDTIQEPSPEPILADTTIIVPLLDFKDVEIRDILNSLAKAYKLNLWLD
                     PSIAGKTTVYFEKVPLNDILRFIIRQNNLEYERTGDIIKIRAKQVIVPAYAQRIEYKD
                     NLLSVDLKGMELEEAVREVSVMTPLQKTIQNMSDSFRWFSAIRPGFDSP"
     gene            1800..3242
                     /locus_tag="VGB16_05175"
     CDS             1800..3242
                     /locus_tag="VGB16_05175"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_020252482.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008270 - zinc ion binding [Evidence IEA];
                     GO_process: GO:0006508 - proteolysis [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="TldD/PmbA family protein"
                     /protein_id="HEX9917111.1"
                     /translation="MEELIKQTIEYLKKKGVDYADLRQIKKEQEDIKVKNKNVETISR
                     SEDQGFGIRVIANGAWGFASSSVINEEEMKRIAEKAVEIAKASAITKKEDVKLSEVEP
                     YKDKFSSKFKTDPMSVSLDKKIELLVKCSEILMKDKRIKVAETGMNSVRTRKFFASTE
                     GAFIEQNIVECGAGMTAMAADGKDVQKRSYPNSFGGDYAMRGYEFIESLKLVDNAERV
                     RVEAIALLGAESCPDTITDIIIAGNQMALQVHESCGHPTELDRVLGTEISLAGGSFMT
                     LDKLNKLKYGSDKVSIVADATLEGGLGSFGYDDEGVKAQKSYLIKNGLFVGYQTSRET
                     AVVLNQTSNGTMRAEGWNRIPLIRMTNINLEPGEWKLEDLIADTKKGLFLDINKSWSI
                     DDKRLNFQFGLEIAWEIKNGKLGKIYKNPVYTGITPQFWNSCDAVCNKNHWHVWGVPN
                     CGKGEPMQVMHVAHGTAPARFKNVKVGVSK"
     gene            3239..4588
                     /locus_tag="VGB16_05180"
     CDS             3239..4588
                     /locus_tag="VGB16_05180"
                     /inference="COORDINATES: protein motif:HMM:NF039907.3"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008270 - zinc ion binding [Evidence IEA];
                     GO_process: GO:0006508 - proteolysis [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="TldD/PmbA family protein"
                     /protein_id="HEX9917112.1"
                     /translation="MKGKDKILSTLDKMVKQSKADHTEAVYIGGESGLTRYANSTIHQ
                     NVSELNSKVYFRTALGKKIGVASTNSLDENNLKKALASALEIAKNQKDNPYFDSLPPK
                     ADYRKIDTYIEETAKFSPLERAERVQKIFEMARPYKLDVAGSFTTGEVEIGVANSRGV
                     VCYQGLTSSAINIIAMSDTSSGYSDALSRDVDEIDVDDLAEAAIKKCLDSRNPKEVEP
                     GEYTVILEPVAVGCLMEWLGYIAFGSKPFLEGTSFIAGNVGKKMMGENITIYDDGNDP
                     SGIAFPFDFEGVPKRKVTFVEKGIAKEVVFNTIDANQGKTKSTGHSLTPDSSEGGFAL
                     NVFIQGGNSSLEKMIQSTEKGILITRFHYINGYLDTRVALMTGMTRDGTFWIENGKVK
                     HGIKNLRFTESMTKAFSNVVALSKERKIVNVWWGDVGCTVTPAMLIKNFKFTGKTEF"
     gene            5057..6871
                     /locus_tag="VGB16_05185"
     CDS             5057..6871
                     /locus_tag="VGB16_05185"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEX9917113.1"
                     /translation="MNSNRITLLILFLALLTLFFSASFPFEAKADQAVILRMEDLDKD
                     EFRSLDFSLSKDTKIDIVAVGAAQRSSQDMYAYAWIINDDNHQLVWEMKKSSTYRYER
                     SKRLREYEGDIQLPPGNYRAYYYVGRIFSTGDYQMQVNSWDEFWDFIRDVIDGTKLEL
                     GEDLGEDLKDLKLVLTTDDKGYVPSLKMHLKTQNSGNSHKEKAKEKPYLVKLNKLGDF
                     RYEKVGFVLDKDMDLDIYALGEYSYTDDRMVDYAWIIDARTRKKVWEMDRANTARAGG
                     GRKNRVFDSTIHLPAGEYILYFVTDDSHSFEGWNVNPPYNPQDWGIAISAKGQELARY
                     FHRKDEVALEKPIIQIVRVGDDQVRNAYFSLKKPMSLYIYALGEASYSDSQMVDYAWI
                     EDANTKEMVWEMTLDNTEYAGGARKNRKFDGIVKLNKGDYKVYYITDDSHSFDDWNSS
                     PPYDPQNWGITISGYGKDFDVSKVEIYDKEKKKPNVLVELVGLGDDQRVSQTFELYQP
                     TKLHIYAIGEGDRDEMYDYGWIKDASSGKTVWQMTYQNTSWAGGAKKNRVYNDNIILD
                     KGSYRCDFTTDDSHSFNNWNAAQPRDPYHWGITVTKLE"
BASE COUNT         2194 a         1326 c         1706 g         1762 t
ORIGIN      
        1 ccttaattca cgagctttgg gaaaactact ggaggcaaag aaaaaataat aggttaggcg
       61 agtacgacgc tcacgaacgt gctataacag aagctgagaa tattgtaaat aatagcgtaa
      121 ggttgtcgcg gtggccatat gagagaccag taataagtac taccacgaga tacggcactg
      181 tctatagcat gaaggcctac cgtcgactga tatatgagca aggtggtaaa ctttatgatg
      241 tatacataaa cctttgcatc ccagaggcta gcaatatcat tagcgttgac gtaattgaaa
      301 ggagttcttc aacaacccaa accgaaaaat aaaaaggaga tagaactatg ggtagagtat
      361 taatcttctt ctcgattttt atattgcttc tggggtgttc taagtattat agacacggag
      421 accgctattc gcataccata ttgattgagg gaatcttcga ggcgagcttg gggtgtgtag
      481 agggttatct gacaactcca caaaacattg tagtcactat gaataaagga agtgactatt
      541 tatgtgaagt gaaagaatca atatcgtttc tggaaaatgt aactggtatt caaacaagca
      601 tgacttatgg gataacagaa gaaaagctct cagaagaaaa ttcaagtttt ctggcaaaag
      661 acttagaaaa atggaaaagg tggtatcagg ataataagga agacatattt cttagcaagg
      721 atagaaaaaa attaatgtat aggaaagagg atgtcaaacg aatagtggat ctgtctgcaa
      781 ctaggtgttg gtatcctttt tgaaaagact catgaaccga gtgatgtgcg tgtactctga
      841 aaagaacccc ttaatttcta cccttaaggc gttttgagaa ttggcagaaa gagataattg
      901 ttgaacagag actgagaaat gatcgggaat cacataaata agccccttct cttgagcata
      961 ggcattacgc ttgttctctt ctccttcctt tcttcaagag cagatgagct taaagtgcag
     1021 gaccaaggac agctgaaatc atcagaagaa gccaaactac ctgacacctc gtctgtcccc
     1081 ccagacacca tacaagaacc atctcccgaa ccaattttag ctgataccac tatcattgtt
     1141 cccttgttag atttcaagga tgttgagatc agggatatct taaactcctt agccaaagca
     1201 tataagctaa acctttggct tgacccgtca atcgctggca agaccacagt ctattttgag
     1261 aaagtccctt tgaatgatat actcagattc attatccgtc agaacaactt agagtatgag
     1321 agaaccggag acataatcaa gataagagca aaacaggtca ttgtgcctgc atatgcccaa
     1381 agaatcgagt acaaagataa cctcctctca gttgacctta aaggcatgga gctggaggaa
     1441 gcggtaagag aggtttcggt aatgactcca ctccagaaaa ctatacaaaa tatgtcggat
     1501 tcgttcaggt ggttctcagc catcagaccg gggttcgact ccccgtagcg ctagtaagat
     1561 agttttgccc tgtaccgttc tggtacaggg ttgagttgcg agttgtgagt tttgagaccc
     1621 accccccacg cgtcaaatta gttttagtct gccaccagtg cttcaattcg gggttcaagt
     1681 cactgcacgc gggtcgtctt ctcactggta aagcagtagc cgaaaatttg cttgacattt
     1741 atttgttttg ggtctaaatt aaaaacctta tactgaaaac aataatcaag gaggagcttt
     1801 tggaagagct gattaaacag accatagagt atttgaaaaa gaagggagtg gattacgctg
     1861 atctcaggca aatcaagaag gagcaagagg atattaaggt taagaacaaa aacgttgaaa
     1921 ctatttcccg aagcgaagac caagggttcg gaattcgggt gatagccaac ggtgcctggg
     1981 gatttgcctc ttcctcggtg ataaacgaag aggagatgaa aagaatagct gagaaagcag
     2041 tggagatcgc caaagccagc gccataacca aaaaggagga cgtaaagcta tcagaagtag
     2101 agccgtataa ggacaaattc tcctccaagt tcaagactga cccgatgtct gtttccttgg
     2161 acaagaagat tgagcttctg gtcaaatgca gtgagatact gatgaaggac aagaggatta
     2221 aggtagcaga aaccggaatg aacagcgttc gcacccgtaa gttcttcgct tcgaccgaag
     2281 gagctttcat agagcagaac atagtggaat gcggtgctgg catgaccgcc atggcagccg
     2341 atggaaaaga tgtgcaaaag cgttcttatc caaattcatt tgggggcgac tatgctatgc
     2401 gcggatacga atttattgaa agcctgaagc tggtggataa tgcggaaagg gtgagagtgg
     2461 aagcgattgc ccttctggga gcggagagtt gtcctgatac aatcactgat attatcatag
     2521 ctggaaatca gatggcgctt caggtgcatg agtcgtgtgg ccatccgact gaattagatc
     2581 gggttctagg aacagagata agtctggctg ggggaagctt catgacttta gataagttaa
     2641 ataaattgaa atacgggtcg gacaaggtaa gcatcgtggc agatgctaca cttgaagggg
     2701 gtttggggag tttcgggtat gacgacgaag gcgtaaaagc ccaaaagtca tatctgataa
     2761 agaatggact ttttgtagga taccaaacct cccgcgagac agctgtggtt ttaaatcaaa
     2821 ccagcaacgg aacgatgaga gcagaaggat ggaatcgcat ccccttgatt cgtatgacca
     2881 acatcaacct tgaaccggga gaatggaaat tggaggacct catcgctgat accaagaagg
     2941 ggttgttttt ggacataaac aaaagctgga gcattgatga taagcgcttg aatttccagt
     3001 ttggtttaga gatagcctgg gagataaaaa acggcaaatt gggcaaaata tataaaaacc
     3061 cggtttatac cggaataact ccccaattct ggaattcctg cgatgcggtt tgcaataaga
     3121 atcactggca tgtatgggga gtgcccaact gcggaaaggg agagccgatg caggtcatgc
     3181 atgtagctca cggaacagca ccagctaggt tcaaaaacgt taaagtggga gtgtcaaaat
     3241 gaagggaaaa gataagatat tatccacctt agataagatg gtgaagcagt ctaaagcaga
     3301 ccatactgaa gcggtttata tcggaggcga gagtgggcta actcgctatg ccaattcgac
     3361 cattcatcag aatgtttcgg aactcaacag caaggtttat ttcagaacgg ctctgggcaa
     3421 aaaaatcgga gtcgcttcaa caaactctct tgacgagaat aacctgaaaa aagctttggc
     3481 gagcgctttg gagattgcca agaatcaaaa ggataatccc tattttgatt cgcttccacc
     3541 taaggcagat tacaggaaaa tagataccta tatagaagaa acagccaaat tctctccttt
     3601 ggaaagagct gaaagagttc agaagatttt cgagatggca agaccgtata aacttgatgt
     3661 tgctggctcc tttaccactg gagaggtaga aataggggta gccaactcca gaggggtagt
     3721 ttgttatcaa ggtttaacct catccgccat taacatcatc gctatgtcag acaccagctc
     3781 cggatacagt gatgccctgt cgcgtgacgt tgacgagata gatgtggatg acttggctga
     3841 agcagctatc aagaaatgtc tggatagcag aaacccaaaa gaagttgagc ctggggagta
     3901 taccgtaatc ttagagccgg ttgcagttgg ctgtctgatg gagtggttag gatatatcgc
     3961 ttttggctca aagccctttt tggaaggaac aagcttcatt gctgggaatg tgggcaaaaa
     4021 gatgatgggg gagaatatca ctatctacga tgacgggaat gacccttcag gtattgcctt
     4081 tcctttcgat ttcgaaggtg tgcccaaaag aaaggttacc tttgtagaaa aaggaattgc
     4141 taaagaagtg gtctttaata ccatcgatgc caatcaaggc aaaaccaagt caactggaca
     4201 ttccttaact ccggattcaa gtgaaggtgg tttcgcttta aacgttttca ttcaaggtgg
     4261 caattcgtct ctggagaaga tgatacagtc aacggagaag ggaatcctga taacgcgctt
     4321 tcattacatc aacggttatt tagataccag ggttgccctg atgaccggaa tgacccggga
     4381 tggaacgttc tggatagaaa atggaaaggt aaagcacggg attaagaatt tacgtttcac
     4441 cgaaagcatg accaaggcat tttccaatgt ggttgcactt tccaaagaga gaaagatagt
     4501 aaatgtctgg tggggggatg tgggttgcac tgtgacgcca gctatgctga ttaagaattt
     4561 caaattcacc ggaaaaaccg agttttaggt tgagaagata gtttagatta tcagcttttg
     4621 tgtaggggcg ggtctttaga cccgccctgt ttttcgggcg gacataaatg tccgccccta
     4681 cgctataaag tcgcaagcga cccaaatggg tcgccttcaa aatacccgcc tgtcattccg
     4741 cacttgatgc ggaatccagt tttgattaaa aaaaccaatg gattcccgct ttcgcgggaa
     4801 tgacaaacaa aaaacatcct cgccccctcc ctctcctttt taaggagagg tttgagaaag
     4861 acaggcgaga ttggtgaata tcagctatat tattttggta ggcacagaaa aaagttcgat
     4921 gaatcgagca acgtaaaatc ttctttctca tatttgtagg gacggaagcc catcccaccc
     4981 tccaattttt tctgaaacca tcctatccag ttgacgtata taaaatgggt aaatctgcct
     5041 ttaacaagga ggaaaaatga actcaaacag aataacctta ctcattttgt ttcttgctct
     5101 gctcaccctc tttttctctg ccagttttcc atttgaagcc aaagcagacc aggctgttat
     5161 actgcgtatg gaagatctgg acaaagatga gttcaggtcc ctagatttca gtctaagcaa
     5221 agatactaaa attgatatag tggcagttgg tgcagctcaa cgctccagtc aggatatgta
     5281 tgcttacgcc tggataataa atgacgataa tcaccagtta gtctgggaga tgaagaaaag
     5341 ctcaacttac cgctacgaga gaagtaaaag attgagagaa tatgaaggag acatccagct
     5401 tccacctgga aattatcgcg cttattatta tgtgggtcgc atcttttcca ccggagatta
     5461 tcaaatgcag gtcaatagct gggatgagtt ttgggacttc ataagagatg tcatcgacgg
     5521 caccaagctt gagctaggcg aagatttagg agaagattta aaggatttga aattagttct
     5581 cactacggat gacaaaggat atgtccccag tctcaagatg catctcaaga cccagaattc
     5641 ggggaattcg cacaaagaga aagccaagga gaaaccatat ctcgttaaac taaacaaact
     5701 cggcgatttt cggtatgaaa aagttggttt tgtcctggat aaagatatgg acttggatat
     5761 ctatgcttta ggagagtatt cctatactga cgatagaatg gttgattacg cttggataat
     5821 cgatgcccgg actagaaaga aagtttggga aatggaccga gctaataccg ccagagctgg
     5881 cggaggcaga aagaatagag tcttcgatag tacaatccac cttccggccg gggaatatat
     5941 tctttatttc gtgacagacg attctcactc ctttgaagga tggaatgtta atccgcccta
     6001 caatccccag gactggggta tagctatttc agctaagggt caggaactgg cgagatactt
     6061 tcacagaaaa gacgaagttg ctctggagaa accgataatt cagattgttc gggttggcga
     6121 cgaccaggtg aggaatgctt atttctccct gaaaaagcca atgagcttat atatttatgc
     6181 tttaggagag gcaagttatt ctgacagcca gatggtggat tacgcctgga tcgaagatgc
     6241 taacaccaaa gagatggttt gggagatgac tctggacaac accgaatatg ccggaggggc
     6301 aagaaagaat cggaaattcg atggcattgt taagttaaac aaaggggatt ataaagtcta
     6361 ctatataacc gatgattccc attcttttga cgattggaat tcttctccgc catacgatcc
     6421 gcaaaactgg ggaatcacca taagcggata cgggaaggat tttgatgtca gtaaagtcga
     6481 gatatatgac aaggaaaaga aaaagccaaa tgtcttagtg gaattggttg gcttgggaga
     6541 tgaccaaagg gtcagccaga cttttgagtt gtaccagccc acaaagcttc atatttatgc
     6601 tatcggtgag ggtgacaggg acgagatgta tgactacggc tggattaagg acgcaagctc
     6661 aggcaagacc gtttggcaga tgacttacca gaatacaagc tgggcgggcg gagcgaagaa
     6721 gaacagagtc tacaatgata atatcatttt ggataaggga agttatagat gtgatttcac
     6781 taccgacgat tcacattctt tcaataattg gaacgcagcc caaccgagag atccttatca
     6841 ttgggggatt actgtgacta agttagagtg agcgtaagtc tgctaagtaa gtctttaatg
     6901 tatagtctga gaatctgaaa acaaaaatgc cccattcttc ttggggcatt tttgacaact
     6961 ttattcccga atcccgtgat gaggataa
//
LOCUS       DASWLN010000158         1937 bp    DNA     linear   ENV 20-FEB-2024
DEFINITION  MAG TPA_asm: Candidatus Zixiibacteriota bacterium isolate
            SMAG_U2949 k141_507737, whole genome shotgun sequence.
ACCESSION   DASWLN010000158 DASWLN010000000
VERSION     DASWLN010000158.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37960594
            Sequence Read Archive: SRR10376091
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Candidatus Zixiibacteriota bacterium (soil metagenome)
  ORGANISM  Candidatus Zixiibacteriota bacterium
            Bacteria.
REFERENCE   1  (bases 1 to 1937)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 1937)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (26-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/12/2024 08:28:26
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 1,440
            CDSs (total)                      :: 1,416
            Genes (coding)                    :: 1,412
            CDSs (with protein)               :: 1,412
            Genes (RNA)                       :: 24
            rRNAs                             :: 1 (16S)
            partial rRNAs                     :: 1 (16S)
            tRNAs                             :: 21
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 4
            CDSs (without protein)            :: 4
            Pseudo Genes (ambiguous residues) :: 0 of 4
            Pseudo Genes (frameshifted)       :: 0 of 4
            Pseudo Genes (incomplete)         :: 3 of 4
            Pseudo Genes (internal stop)      :: 1 of 4
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..1937
                     /organism="Candidatus Zixiibacteriota bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_507737"
                     /isolate="SMAG_U2949"
                     /isolation_source="soil"
                     /db_xref="taxon:2053527"
                     /environmental_sample
                     /country="USA: Oregon"
                     /lat_lon="42.99 N 123.63 W"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            <1..931
                     /locus_tag="VGB16_05190"
     CDS             <1..931
                     /locus_tag="VGB16_05190"
                     /inference="COORDINATES: protein motif:HMM:TIGR04183.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0031240 - external side of cell outer
                     membrane [Evidence IEA]"
                     /codon_start=2
                     /transl_table=11
                     /product="T9SS type A sorting domain-containing protein"
                     /protein_id="HEX9917114.1"
                     /translation="QVAIIHSEELLDNCPLGHSYNNTYSVLDWYLKAGGKVIIEGRRN
                     LTDENGKCGYIGPGTECLRDSSELQSFIRQNLHINQVFIPLWDSRNEEFIGAFSQIPE
                     YPELQTDSIRVEGSIYPPFLDGIPLGGKLPLIGWFMPDDSAEVIYTFNSAYDTSQQEG
                     KPVALRYLGDTYKLVYFDFPLYFIKEDQATQLLHQALADLNQYIGVEDDDKKMNLPSQ
                     FALAQNYPNPFNSQTAIEYSLPRASEVKIAIYNVIGQRVKRLVNDEEQPAGHKRVIWD
                     GKNDRAEIVSSGIYFYRIETEGFVQSKKMLLLK"
     gene            1269..>1937
                     /locus_tag="VGB16_05195"
     CDS             1269..>1937
                     /locus_tag="VGB16_05195"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011928343.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0000166 - nucleotide binding [Evidence
                     IEA];
                     GO_function: GO:0004812 - aminoacyl-tRNA ligase activity
                     [Evidence IEA];
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_process: GO:0043039 - tRNA aminoacylation [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="amino acid--tRNA ligase-related protein"
                     /protein_id="HEX9917115.1"
                     /translation="MEELNELMQQRREKLRKIIDKKINPYPYRFERTHLSKQILDDFE
                     NLEGKETKIAGRIISFRSHGKSTFFHILDGEGKIQVYVKEDEVGRENYEFLDLLDLGD
                     FVGIEGKPFKTKTGEITVKAKSLVLLSKSLHTLPEKWHGLQDKELRYRQRYVDLFVNP
                     QVKEVFIKRTMLITALRRYLDGLGFIEVETPILQPIYGGALARPFKTHHHSLDMDLYL
                     RIADE"
BASE COUNT          610 a          344 c          433 g          550 t
ORIGIN      
        1 ccaggttgct attatacatt ctgaggaatt acttgataat tgtcctttag gccatagtta
       61 caacaatact tactcggttt tagattggta tttaaaagca gggggcaagg ttattataga
      121 aggaaggaga aacctaacag atgaaaatgg gaaatgtggt tatatcggcc ctgggacaga
      181 gtgtctgaga gattcttctg aacttcagag ttttattcgg cagaatctgc acatcaatca
      241 agtctttatt ccattgtggg atagtagaaa cgaggagttc attggggctt tcagtcaaat
      301 acccgaatat ccggaattac agactgatag cattagggtt gaagggtcaa tctaccctcc
      361 ttttctcgat ggtattccct tgggtggcaa actccctctc attggctggt ttatgccgga
      421 tgattcagct gaggttattt atacctttaa ctcagcttat gatacttccc aacaagaagg
      481 aaagccagtg gctttaagat atttgggaga tacttacaag ctggtctatt ttgactttcc
      541 tttatacttc atcaaagaag accaagctac gcaacttctg catcaagcct tagcagattt
      601 gaatcaatat attggagtag aagatgacga caaaaagatg aaccttccat cccaattcgc
      661 cttagcacaa aactatccta accccttcaa ttcccagaca gcgattgaat actctctgcc
      721 cagagcaagt gaagtgaaga tagctatcta caatgttatt ggacaaagag taaaaaggtt
      781 ggtgaatgat gaagagcagc ctgctggtca taagagggta atctgggatg gaaagaacga
      841 cagagcagag attgtctcaa gtggaatcta cttctacagg atagaaacgg aagggtttgt
      901 tcagtcgaag aagatgctgc ttttgaaata ggtcaggtca caacagtccg gtttgataaa
      961 gagccacctg attttgggtg gctctttact tatcttagtc tttatcacca agatgagcaa
     1021 gacaactgtg aggacgacca gacaaacaag acaagaggta aactctttgg gattactaca
     1081 acttaagaga tagaaatctt tcgagtcgtg ctgggatcta tagcctcaat attccttccc
     1141 ttctccaatt cagggcaact tctgatctac tgactctgat tagctttctt aaagttcgct
     1201 tgacatgaac ttgggtttta cttatattca aaaaaatggg gaattgctaa tttcaggaga
     1261 gtaaaaaagt ggaagagcta aatgaattga tgcaacaacg ccgggaaaag ctaagaaaga
     1321 taatagataa gaagataaat ccttatcctt atagattcga gcgcacccac ctttccaagc
     1381 agattttgga tgattttgag aatctggaag gaaaagaaac gaaaatagct ggcagaatca
     1441 tttctttccg ttctcatggc aaaagcacat ttttccatat tttggatgga gagggaaaaa
     1501 ttcaagttta tgttaaagag gatgaggttg gaagggaaaa ttacgagttt ttagatctgt
     1561 tggatctggg tgatttcgtg ggaatagaag gtaagccctt taaaaccaaa accggtgaaa
     1621 taaccgttaa agcaaagagt ttggtattgt tatccaagtc ccttcatacc cttccagaaa
     1681 aatggcatgg cttgcaggat aaggaattaa gatatagaca aagatacgtt gaccttttcg
     1741 tcaacccgca agtcaaagag gtcttcatta aaaggactat gctaatcact gcgttgagaa
     1801 gatatttgga tggactggga tttattgaag ttgaaactcc tatcttgcag ccgatttacg
     1861 gtggtgcttt agcccgacca tttaagaccc atcaccatag tctggatatg gatttgtatt
     1921 tgagaattgc ggatgag
//
LOCUS       DASWLN010000159         2206 bp    DNA     linear   ENV 20-FEB-2024
DEFINITION  MAG TPA_asm: Candidatus Zixiibacteriota bacterium isolate
            SMAG_U2949 k141_1095714, whole genome shotgun sequence.
ACCESSION   DASWLN010000159 DASWLN010000000
VERSION     DASWLN010000159.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37960594
            Sequence Read Archive: SRR10376091
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Candidatus Zixiibacteriota bacterium (soil metagenome)
  ORGANISM  Candidatus Zixiibacteriota bacterium
            Bacteria.
REFERENCE   1  (bases 1 to 2206)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 2206)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (26-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/12/2024 08:28:26
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 1,440
            CDSs (total)                      :: 1,416
            Genes (coding)                    :: 1,412
            CDSs (with protein)               :: 1,412
            Genes (RNA)                       :: 24
            rRNAs                             :: 1 (16S)
            partial rRNAs                     :: 1 (16S)
            tRNAs                             :: 21
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 4
            CDSs (without protein)            :: 4
            Pseudo Genes (ambiguous residues) :: 0 of 4
            Pseudo Genes (frameshifted)       :: 0 of 4
            Pseudo Genes (incomplete)         :: 3 of 4
            Pseudo Genes (internal stop)      :: 1 of 4
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2206
                     /organism="Candidatus Zixiibacteriota bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_1095714"
                     /isolate="SMAG_U2949"
                     /isolation_source="soil"
                     /db_xref="taxon:2053527"
                     /environmental_sample
                     /country="USA: Oregon"
                     /lat_lon="42.99 N 123.63 W"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            complement(<1..1172)
                     /locus_tag="VGB16_05200"
     CDS             complement(<1..1172)
                     /locus_tag="VGB16_05200"
                     /inference="COORDINATES: protein motif:HMM:NF024240.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="FAD-dependent oxidoreductase"
                     /protein_id="HEX9917116.1"
                     /translation="MEKGSIRTIPEPNKEVKVFRECDVVVVGGGPGGIGAAIAAARNG
                     ADTVLIERYGYVGGMGTGGLVTIIPCLSDFSGQMRIAGLNQEWIERLKNREAVTYPPP
                     EIWGTDDKGHVSYWNERAFFFVRENRVVYAAFIDAEISKCVLADMLNEAKVKTCFHSW
                     GTMPIMNGDKAVGVIFESKSGRQAVLAKVVIDSTGDGDLLPFTKAKFDVDIDPNIRIA
                     NLSLCFWVDGVDYVKVADFKNQDPERWVDLINNLRKQGGSGFFRSNLKNQENILWFHP
                     RYACSSQIDVEELTRVEFLGRQKMLLMHDYFKKNVPGFENSFIVLSAPQLGTRASRRV
                     HGDYMLTAKDLASDQIFDDTIATFPDLDRGEESIKHPLTYIPYRSLLPKGVENMLVA"
     gene            complement(1244..>2206)
                     /locus_tag="VGB16_05205"
     CDS             complement(1244..>2206)
                     /locus_tag="VGB16_05205"
                     /inference="COORDINATES: protein motif:HMM:NF012706.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0055052 - ATP-binding cassette (ABC)
                     transporter complex, substrate-binding subunit-containing
                     [Evidence IEA];
                     GO_function: GO:0042626 - ATPase-coupled transmembrane
                     transporter activity [Evidence IEA];
                     GO_function: GO:0140359 - ABC-type transporter activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="ABC transporter substrate-binding protein"
                     /protein_id="HEX9917117.1"
                     /translation="GPFMFDSWQRDNYLRVKKNPNYWQKGQPYLDGIEFRNVSDVTIS
                     IMSFKAKETNCVLTIDPIDAVNLKKEGYQIVVPPMHLIHSLCPDGANPNSPFANLKVR
                     QALEYAIDKKGMAEGIGMGYFEPVTQFAAKSDGWYDPNIPAREYNVQKAKQLLAEAGY
                     PNGVKTKLVSDVRVRSDTLVAVQTYLKEAGFDCTLDKADAARSMELTVKGWEGILMPG
                     FPTAVNTAALTARFYTPNYPSMYRPAGWNQKWEAMLSQIDDKERIKQLKELVRIMFDE
                     VMIIPYQSDSSRAVLDGTVVGFAETYQINNNADYWEPGIVYLTK"
BASE COUNT          596 a          517 c          433 g          660 t
ORIGIN      
        1 gcaaccagca tgttttccac accctttgga agtaaagaac gatacggaat ataggtcaac
       61 ggatgtttga tagattcctc tcctctatcc aggtccggaa atgtggcaat ggtatcatcg
      121 aaaatttgat cggaggctaa gtccttagca gtgagcatgt aatcaccgtg aactcttcga
      181 gaagctctgg tacccaattg tggagcactt aatacaataa agctattttc aaagcccggg
      241 acatttttct taaagtaatc atgcattaat aacatttttt gacggcctag gaattcgaca
      301 cgggtgagtt cctcaacatc tatctggctg gaacaagcat aacgtggatg gaaccatagg
      361 atattttctt gattcttcaa attacttctg aagaaacccg atccaccttg tttcctaaga
      421 ttgtttatta aatcgaccca tcgttcggga tcttgatttt tgaaatctgc cactttcaca
      481 taatcaacgc catccaccca aaaacataag gacaaattgg cgattcttat attaggatca
      541 atatcaacat caaatttagc tttggtaaaa ggcagtaaat ctccgtcacc ggtggaatca
      601 atgactactt tggctaaaac agcttgccgg cctgatttgc tctcaaaaat tacgcccact
      661 gccttgtctc cattcataat gggcatcgtg ccccaggaat gaaaacaagt tttgactttg
      721 gcctcattta gcatgtccgc cagaacacat tttgaaattt cagcgtcaat gaaggctgca
      781 tagaccaccc tattttcacg cacaaagaag aatgcccttt cattccaata actaacatgt
      841 cccttgtcat cagtgcccca aatctctggg ggtggatagg ttacggcttc tctatttttc
      901 agtctttcaa tccattcttg attgagaccg gcgattcgca tttgacctga aaaatcagac
      961 aaacaaggga ttattgtgac aagaccacca gtacccattc ctcccacata tccgtatctt
     1021 tcgataagaa cagtgtctgc accgtttcgg gctgcggcta tggctgcacc gataccacca
     1081 gggccacccc ccactacgac aacatcgcat tcccggaata ccttaacttc tttattgggt
     1141 tcaggaattg ttctgattga tcctttctcc atgctgattt ctccttgtaa atcgaaaaaa
     1201 atcacagcca ctatcgacct gggttagttt tagggtatct ggattattta gtcaggtaaa
     1261 ctattcctgg ttcccaataa tcggcattat tattaatttg atatgtttcg gcaaaaccta
     1321 ctacagtccc atccaggaca gcacgggaag aatcactctg gtaggggatt atcatgacct
     1381 catcaaacat tattctaact aattctttta actgttttat acgttcctta tcatctattt
     1441 gggataacat ggcttcccat ttttgattcc atccggcggg gcgatacatg ctgggatagt
     1501 taggagtgta gaagcgggcc gttaatgctg ctgtgtttac tgcggtgggg aaacccggca
     1561 taaggatgcc ttcccagcct tttacagtta attccataga tctggcagcg tctgctttat
     1621 ccagggtgca atcaaatcca gcttctttca gataagtctg tactgctacc aatgtatccg
     1681 agcgtactct gacatctgag accaatttgg ttttgacgcc gttaggatag ccagcctctg
     1741 ccagcaactg cttggctttt tggacattat attctcgagc cggtatattg gggtcatacc
     1801 aaccgtcaga ttttgcagca aattgagtca ccggctcaaa gtaacccatt ccgatgcctt
     1861 ctgccatacc ttttttatcg atggcgtatt ccagtgcttg acggaccttg agattggcaa
     1921 agggagaatt cggattggcc ccatccggac ataagctgtg aattaaatgc attgggggaa
     1981 ctacaatctg atagccttct ttctttaagt taacggcatc tatcggatca atcgtcaaca
     2041 cgcaattggt ttctttagcc ttaaaagaca tgatgctgat agttacatcc gaaacattac
     2101 ggaactctat cccatccaga taaggctggc ctttttgcca gtaatttgga tttttcttta
     2161 ctcttagata gttatctctc tgccagctat caaacatgaa aggacc
//
LOCUS       DASWLN010000160        16985 bp    DNA     linear   ENV 20-FEB-2024
DEFINITION  MAG TPA_asm: Candidatus Zixiibacteriota bacterium isolate
            SMAG_U2949 k141_207496, whole genome shotgun sequence.
ACCESSION   DASWLN010000160 DASWLN010000000
VERSION     DASWLN010000160.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37960594
            Sequence Read Archive: SRR10376091
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Candidatus Zixiibacteriota bacterium (soil metagenome)
  ORGANISM  Candidatus Zixiibacteriota bacterium
            Bacteria.
REFERENCE   1  (bases 1 to 16985)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 16985)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (26-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/12/2024 08:28:26
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 1,440
            CDSs (total)                      :: 1,416
            Genes (coding)                    :: 1,412
            CDSs (with protein)               :: 1,412
            Genes (RNA)                       :: 24
            rRNAs                             :: 1 (16S)
            partial rRNAs                     :: 1 (16S)
            tRNAs                             :: 21
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 4
            CDSs (without protein)            :: 4
            Pseudo Genes (ambiguous residues) :: 0 of 4
            Pseudo Genes (frameshifted)       :: 0 of 4
            Pseudo Genes (incomplete)         :: 3 of 4
            Pseudo Genes (internal stop)      :: 1 of 4
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..16985
                     /organism="Candidatus Zixiibacteriota bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_207496"
                     /isolate="SMAG_U2949"
                     /isolation_source="soil"
                     /db_xref="taxon:2053527"
                     /environmental_sample
                     /country="USA: Oregon"
                     /lat_lon="42.99 N 123.63 W"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            369..884
                     /gene="nuoE"
                     /locus_tag="VGB16_05210"
     CDS             369..884
                     /gene="nuoE"
                     /locus_tag="VGB16_05210"
                     /EC_number="1.6.5.9"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_013720430.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016491 - oxidoreductase activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="NADH-quinone oxidoreductase subunit NuoE"
                     /protein_id="HEX9917118.1"
                     /translation="MDANLTKVEAIVDRYKVEDSDLTKVEAIVDRYKGEGGALIAILQ
                     DVQAEYNYLPPDALKGIAEKLGIPLILVYGIATFYKAFSLKPRGKHLVTVCTGTACHV
                     RGAKRILAEVQKKLNVKRGETTEDKMFTLETVNCLGCCAIGPVMVVDGEYNGQTTTAK
                     ANSILEKYKSR"
     gene            1029..4172
                     /gene="nuoF"
                     /locus_tag="VGB16_05215"
     CDS             1029..4172
                     /gene="nuoF"
                     /locus_tag="VGB16_05215"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_017872372.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008137 - NADH dehydrogenase (ubiquinone)
                     activity [Evidence IEA];
                     GO_function: GO:0010181 - FMN binding [Evidence IEA];
                     GO_function: GO:0051287 - NAD binding [Evidence IEA];
                     GO_function: GO:0051539 - 4 iron, 4 sulfur cluster binding
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="NADH-quinone oxidoreductase subunit NuoF"
                     /protein_id="HEX9917119.1"
                     /translation="MRKLKNIKDLDELKEGLSKQKPTDKPCIIISSGTCGQARGSAKV
                     VEAFEQKIRSNGFKDKVAIRVTGCHGFCEVEPTVLVYPEGILYQKVKPEDADQIISET
                     IINHKVIDRLLYSDPVTQQKFIHEEEFPFYRKQNRLVMGNNRFVDPTKIEDYLAVGGY
                     SGLRKILSGMTSEQVIFEVKKSGLRGRGGGGFPTGLKWELCKKAKGQIKYIICNADEG
                     DPGAYMDRSLLEGNPHSVMEGMLIGAFAIGAKEGYIYVRNEYPLAVKNVGIAINQAKE
                     YGLLGEDILDSGFDFDLKIVRGAGAFVCGEETALIASIEGRLGEPKQRPPYPVQKGLW
                     GKPTNINNVETWANVPLIIDRGAEWYSKIGTEKSKGTKIFSLVGKINNTGLVEVPMGI
                     TLREIIEDIGGGIPGGRKFKAVQTGGPSGGCMPAKLLDLPVDYEKLTGVGSMMGSGGM
                     VVMDEDTCMVDVAKYFLNFLKDESCGKCFTCREGIIRMLEIVTDITDGKGTMADIHLL
                     EELAKAVKDSTMCGLGQTSPNPVLSTLKYFRDEYEAHVKYKRCPAVVCKKIISSPCQH
                     TCPINQQGCVYIALIARGEFAKAIDIIRKDNPLPSVCARVCHHPCESKCRAGESGEPI
                     SIRALKRFAIDYGLANGIKPKNKFQKTREEKVAIIGSGPAGLTCGFYLAQKGYEVTIY
                     EEAPVAGGMLALGIPEHRLPRKILNADIEAIKTAGVIIKTNQTLGKDFSLDELFKKGY
                     KAVFIATGAHKSWKLGIPGEEGEGVLESMKFLVAVNLGKEVKIGKRVGIIGGGNAAVD
                     SARVANRFSGCERVTIIYRRTKTEMPAFKERGGKKEVEDALEEGIDIQLLTAPVKVLR
                     ENGKLVGIECIRMKMGEPDESGRRRPVPIPGSEFKIELDTLILAIGEQPDISFLTENS
                     GVGISKSGTIVVDPETLTTNKEGVFAGGDVVSGANTVVEAMAAGKVASESIDKYLRGE
                     SLTRVYEVTKPSILVEALELTEQELAELKRPEMPALSVKQRHKSFQEVELGFDEKMAI
                     KEAKRCLRCELESEEIE"
     gene            4203..4880
                     /locus_tag="VGB16_05220"
     CDS             4203..4880
                     /locus_tag="VGB16_05220"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_020259852.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="2Fe-2S iron-sulfur cluster-binding protein"
                     /protein_id="HEX9917120.1"
                     /translation="MLSLKLNGVEVQAEEGWTLLETARFYGLPIPTLCYHEGLSPYGA
                     CRLCVVEIGEGPTSKLVSSCTYPAEEGLVVRTHSKRVEQARKMVVELLLATCPDSRTI
                     QDLAASMEVQRTRFKVRHDDCILCGLCVRMCTEQMQGKAIGFVNRGELRKITTPFDIR
                     SEDCRLCGGCMYICPACQLRCQGPDAPGVVCGSCLNVSPVCLEEHEDALCYLDPCCQC
                     LKEEVKK"
     gene            4953..6536
                     /locus_tag="VGB16_05225"
     CDS             4953..6536
                     /locus_tag="VGB16_05225"
                     /EC_number="1.4.-.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_020259853.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0015930 - glutamate synthase activity
                     [Evidence IEA];
                     GO_function: GO:0016638 - oxidoreductase activity, acting
                     on the CH-NH2 group of donors [Evidence IEA];
                     GO_process: GO:0006537 - glutamate biosynthetic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="FMN-binding glutamate synthase family protein"
                     /protein_id="HEX9917121.1"
                     /translation="MSLTRLNATAATLTKNRTEESVSHFSGMCVTCVDGCIGMCEIGK
                     SAYRGAEVIYPQPFGIITAASEKNYPVDLSHFTIMGTAVGAKGIAEDSNVAIFPNANL
                     ERKIGNDPKIKLRLPFIIPGLGSTNIAKNNWEGLAIGAALAGIPLTIGENVCGMDVES
                     VIENGKVKHAPDLAWRVKLYKDWQQDGYGAIVLQANIEDTALGVLEYGLTKLGVDVVE
                     LKWGQGAKDIGGEVKISSLQKAQLLKKRGYVVLPDPLDPANIDAFEKGSFKEFERHSR
                     VGMVTEEGFMKRVKELRDAGAKHIFLKTGAYRPADLARAIKFSSKARLDLLTVDGAGG
                     GTGMSPWRMMNEWGIPSVELHSLLYQYANKLAKKGEYVPDIAIAGGFTFEDQIFKGLA
                     LGAPYFKLIGMARGPLAAAMVGKTIGKRINEGMVPVFIERFGNTPEEIFVSAPELKRK
                     LGEATFRKVPTGALGLYTYMQRLAQGLRQLMSGNRKFALEYITRDDIAALTKEASEIS
                     GISYVMDADKEEAEKILKS"
     gene            6887..7405
                     /locus_tag="VGB16_05230"
     CDS             6887..7405
                     /locus_tag="VGB16_05230"
                     /inference="COORDINATES: protein motif:HMM:NF014657.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF177 domain-containing protein"
                     /protein_id="HEX9917122.1"
                     /translation="MRLELSKLKQEKVNFQFTESAEELDLRADGVEFGSPLKVKVEVS
                     PSGESFLAWAKIETRVKLECVRCLLIYSYPLQAEFNLLIEKQRPGLLLDPGNEDTVVI
                     NPQDKFIDISDRIRQAIALSLPLKSLCSPDCKGLCDICGADLNTSPCSCTIEEYDSRW
                     DKLKQALKKERD"
     gene            7407..7589
                     /gene="rpmF"
                     /locus_tag="VGB16_05235"
     CDS             7407..7589
                     /gene="rpmF"
                     /locus_tag="VGB16_05235"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_018211004.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0000315 - organellar large ribosomal
                     subunit [Evidence IEA];
                     GO_component: GO:0022625 - cytosolic large ribosomal
                     subunit [Evidence IEA];
                     GO_function: GO:0003735 - structural constituent of
                     ribosome [Evidence IEA];
                     GO_process: GO:0006412 - translation [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="50S ribosomal protein L32"
                     /protein_id="HEX9917123.1"
                     /translation="MPLPKRRHSKTRGAKRRTHWKLGSPNVIECPHCHQPKLSHRVCP
                     HCGYYKEKEIIIPGKS"
     gene            7599..8639
                     /gene="plsX"
                     /locus_tag="VGB16_05240"
     CDS             7599..8639
                     /gene="plsX"
                     /locus_tag="VGB16_05240"
                     /EC_number="2.3.1.274"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_013120927.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0043811 - phosphate:acyl-[acyl carrier
                     protein] acyltransferase activity [Evidence IEA];
                     GO_process: GO:0008610 - lipid biosynthetic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="phosphate acyltransferase PlsX"
                     /protein_id="HEX9917124.1"
                     /translation="MADKVRIGLDAMGGDKAPASIVEGALLASRDRKADYQVVLVGDK
                     GKIQRELERLGVSVPPFEVVHASEVVSMDEPAAEAARKKKDSSLAVGMGMQKEGKLDG
                     FVSGGNTGAVMATAVLTLGHLEGVDRPAIASYFPSEKDAVVVLDVGANADCKARNLYE
                     FGVMGTIYTNYILKRENPRLGLLSIGEESTKGNELTLVSYRLLSESKLNFIGNVEGHD
                     VLKGTCDVVVCDGFVGNIMLKFAESIESFLYFLIKEELRRSILFRTGAFLLKYPLRSL
                     RKSLNYAEYGGAPLLGVDGVCIICHGKSSPKAIKNAVKLASKMVNEKVNEHIKEELRN
                     SYQLEGKNGKSL"
     gene            8623..9612
                     /locus_tag="VGB16_05245"
     CDS             8623..9612
                     /locus_tag="VGB16_05245"
                     /EC_number="2.3.1.180"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_012995333.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004315 - 3-oxoacyl-[acyl-carrier-protein]
                     synthase activity [Evidence IEA];
                     GO_function: GO:0016746 - acyltransferase activity
                     [Evidence IEA];
                     GO_function: GO:0033818 -
                     beta-ketoacyl-acyl-carrier-protein synthase III activity
                     [Evidence IEA];
                     GO_process: GO:0006633 - fatty acid biosynthetic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="beta-ketoacyl-ACP synthase III"
                     /protein_id="HEX9917125.1"
                     /translation="MANRYNARIVGTGMAVPKRVLTNAELEKMMDTSDEWITARSGIK
                     ERRIADENTAASDLALEASLKAMEEAKLKPEDLDLIIVGTVTPDFTLPSTACILQDRL
                     GNKTAGVMDVVAACSGFIYGLSVAKAFVNIGQFKNVLVIGVEILSKFTNWDDRNTCVL
                     FGDGAGAAIVQASEEEGGIISTYISGDGSLANLLHAPIGGTRTRLTKENFDLKQHNII
                     MAGSEVFKSAVRAMGDSAVKVIEQSGLKPEDIDLLIPHQANIRIIEATARRLKMPMDK
                     VYVNIDKYGNTSSASVPIALDEARRKGKIKKGDKTVLVAFGGGFTWGAALIQW"
     gene            9618..10559
                     /gene="fabD"
                     /locus_tag="VGB16_05250"
     CDS             9618..10559
                     /gene="fabD"
                     /locus_tag="VGB16_05250"
                     /EC_number="2.3.1.39"
                     /inference="COORDINATES: protein motif:HMM:NF012901.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004314 - [acyl-carrier-protein]
                     S-malonyltransferase activity [Evidence IEA];
                     GO_process: GO:0006633 - fatty acid biosynthetic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="ACP S-malonyltransferase"
                     /protein_id="HEX9917126.1"
                     /translation="MKNFAGIFPGQGSQYVGMARDLYDAYLHIKEFYKKADDVLGYGL
                     SRVCFEGPEKTLTETKYTQPAIFVHSLALWNLMSERKKIRPSFVAGHSLGEYSALVVA
                     GVFSFEDGLFAVKQRASLMQEACDRNKGTMAAIIGLSREDVLEICKEASSKGIIQPAN
                     FNSEEQIAISGEMEAVEFGVELAQRKGAKRAMLLQVGGAFHSPLMNSAREGMSEVLSK
                     VEINPARIPVVSNVTARVMTQPQEIKDALVEQITKPVLWYDSMQYVYQQGVRDFVEIG
                     PGKVLQGIIKRSFNDASNFGVDKSVDLDKFLELLEIA"
     gene            10708..11448
                     /gene="fabG"
                     /locus_tag="VGB16_05255"
     CDS             10708..11448
                     /gene="fabG"
                     /locus_tag="VGB16_05255"
                     /EC_number="1.1.1.100"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_013150368.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004316 - 3-oxoacyl-[acyl-carrier-protein]
                     reductase (NADPH) activity [Evidence IEA];
                     GO_process: GO:0006633 - fatty acid biosynthetic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="3-oxoacyl-[acyl-carrier-protein] reductase"
                     /protein_id="HEX9917127.1"
                     /translation="MNLKDKTAIVTGGAQGIGKAICERLAGEGTKIAVFDIDLDSATS
                     LAQSIKDRGGLAQALKIDVSSFQEVEQGVNKVIEQFSTIDILVNNAGITKDNLFIKMF
                     EEEWDRVIQINLKGAFNLCKSVARIMLKQKSGKIINISSVIGIMGNVGQANYSASKAG
                     LLGLTKSLAKELASRGITVNAVAPGYIQTQMTESLSQAARDAFLNSIPLRRAGQPEEV
                     ANLVAFLASEEANYITGQVFQIDGGLLM"
     gene            11490..11732
                     /gene="acpP"
                     /locus_tag="VGB16_05260"
     CDS             11490..11732
                     /gene="acpP"
                     /locus_tag="VGB16_05260"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_012462771.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_process: GO:0006633 - fatty acid biosynthetic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="acyl carrier protein"
                     /protein_id="HEX9917128.1"
                     /translation="MSVEQRVKEIIVEQLGVDPEQVTNEASFVDDLGADSLDTVELVM
                     ALEEEFGLEIPDEEAEKITTVGEAIEYIEKNAVEGN"
     gene            11777..13018
                     /gene="fabF"
                     /locus_tag="VGB16_05265"
     CDS             11777..13018
                     /gene="fabF"
                     /locus_tag="VGB16_05265"
                     /EC_number="2.3.1.179"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003542975.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004315 - 3-oxoacyl-[acyl-carrier-protein]
                     synthase activity [Evidence IEA];
                     GO_process: GO:0006633 - fatty acid biosynthetic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="beta-ketoacyl-ACP synthase II"
                     /protein_id="HEX9917129.1"
                     /translation="MKNRVVVTGMGAITPVGNSVDVFWEALKNGVSGVDKVSRFDVAN
                     YPTKIAAEIKGFDTSAQIDKKEARRMDLYEQYAIVASQQVVDDAGLDLNSMDKSRAGV
                     VIGSGIGGIETFEKQCSTMISSGPGRVSPFFIPMMIIDMCAGLVSIRFGFTGPNYATV
                     SACASSAHAVADAFRIIQRGEAEVMITGGSEAAITPLSFAGFCSARAMSTRNDEPEKA
                     SRPFDKQRDGFVMGEGAGMLILESLEHAQKRGAKIYAEIAGVGMSADAYHITAPSPGG
                     EGAVRSMQAAIQDAGVSPQEVGYINSHGTATDLGDISETVAIKKVFGEHAKKIAINST
                     KSMTGHLLGAAGAVELIVGIKSMSEGVIHPTINLEFPDPECDLDYVPNLKREKEINVF
                     LSNSFGFGGHNVTLVVRKFVP"
     gene            13075..13845
                     /gene="rnc"
                     /locus_tag="VGB16_05270"
     CDS             13075..13845
                     /gene="rnc"
                     /locus_tag="VGB16_05270"
                     /EC_number="3.1.26.3"
                     /inference="COORDINATES: protein motif:HMM:TIGR02191.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004525 - ribonuclease III activity
                     [Evidence IEA];
                     GO_process: GO:0006396 - RNA processing [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="ribonuclease III"
                     /protein_id="HEX9917130.1"
                     /translation="MSPFRYIKKIFSKSSQLGKLEKMTGYHFNNLNLLNQALIHRSII
                     KDKSTQVLQPNERLEFLGDAVLGLVVSRFLYEKFPEKREGDLTKLKAVLVSESTLRRI
                     AKDINLGQFLQLSEEENKAGGRERSSILADAYEALIGATFLDGGLAPARKMIRNQLLK
                     KYWELTTDEEHFNYKGELLEYLQSFGWGMPRYEIVEEKGPDHQKKFTIDVFAQGKRMG
                     RGTGTSKKEAEQKAAKKTLENINKGELLNRQVNGLSPD"
     gene            14073..14195
                     /locus_tag="VGB16_05275"
                     /pseudo
     CDS             14073..14195
                     /locus_tag="VGB16_05275"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_013780941.1"
                     /note="incomplete; partial in the middle of a contig;
                     missing N-terminus; Derived by automated computational
                     analysis using gene prediction method: Protein Homology.
                     GO_function: GO:0016627 - oxidoreductase activity, acting
                     on the CH-CH group of donors [Evidence IEA]"
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="acyl-CoA dehydrogenase family protein"
     gene            14721..15485
                     /locus_tag="VGB16_05280"
     CDS             14721..15485
                     /locus_tag="VGB16_05280"
                     /inference="COORDINATES: protein motif:HMM:NF015084.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="biotin/lipoate A/B protein ligase family
                     protein"
                     /protein_id="HEX9917131.1"
                     /translation="MATDETLTYLCQEENCIPTLRLYTWSPPAVSLGYFQKIESAVNM
                     DECIKLGIDVVRRPTGGRAVLHQDELTYSVCGSGENFPELGTTVSQTYERISLALLET
                     LRILGIKGEWVRSLPRQKKEKISEPCFVSSSKYEITVDNKKLIGSAQRRFGKAFLQHG
                     SIPLKRNNKSLAHFLPQRKSVDAMDKLLTTRSTTIEELLNREVKIESIIEAIKSGFKG
                     YFEIEFMEENLTDNELDRIEKLISEKYYTNQTNPAK"
     gene            15964..>16985
                     /locus_tag="VGB16_05285"
     CDS             15964..>16985
                     /locus_tag="VGB16_05285"
                     /inference="COORDINATES: protein motif:HMM:NF019335.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0015267 - channel activity [Evidence IEA];
                     GO_process: GO:0015891 - siderophore transport [Evidence
                     IEA];
                     GO_process: GO:0055085 - transmembrane transport [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="TonB-dependent receptor"
                     /protein_id="HEX9917132.1"
                     /translation="MRCYKNANIVAIVFLGIFSLIFLSVSSYAGVSGKISGKVIDKNS
                     KMPLPGASIQIGGTSMGNYSGPDGSYFILNVAPGTYDVTAKLIGYRIVEVKGVKVATD
                     KTTEQDFELTVSAVELKEVVEVIGRAEVIEKDVTTNVRTVSSDQIQNMAVKEVADILK
                     TQVGFVTKNMELHIRGGRAGEVLYIVDGVETKDPLGGLGIVRGGMEVSADNIEEISVL
                     KGGFDAEYGNVQSAVINLVTKEGSIQKTTGHVEFLTDDFGDKRLNKYSFNSDRIEMSL
                     SGPEPILSRFLVPLTEKFSREKLAYRLGLDAYKTDTYYDINKYSTPSAERKFVSYNIL
                     GIDVPD"
BASE COUNT         5042 a         3155 c         4499 g         4289 t
ORIGIN      
        1 acctgcaagc ttcctgaatc tgaaggcggc gtttggcatt gtgaggaata tcaataaaac
       61 ataggcgtat aatataagtt aaagcaacga aagagaagta gaaaggagca actgaacatt
      121 cgtcatgtct tggctaaaac catcaacgat atagtagtca attttccggt caggatggat
      181 gagggacaaa ttgagatatt tactggatat tgagttcagc acaatcatat actcgggcct
      241 ttctaagacg gaactcgttt tcatcatgcc atcaacaagg acaatttatg ttatattcgt
      301 tcaaaatttc acaaggtctt ccgatcttgc gcatatcaat tcacaacttt tctaaggaga
      361 gataaattat ggatgcaaat ttgaccaaag tggaggccat cgttgacaga tataaggtag
      421 aggattcaga tttgaccaaa gtggaagcta tcgttgacag atataagggt gaaggcggcg
      481 ctttgattgc tatattgcag gatgttcagg cggagtataa ttatcttccc ccagatgctt
      541 tgaaagggat agctgaaaaa ttgggcattc ccctgatatt ggtctatggg attgccacgt
      601 tttataaagc tttcagcctt aagcccagag gaaagcatct ggttacggtc tgcacgggaa
      661 ctgcctgtca cgtaagaggg gcgaaaagga tattagcaga agtccaaaag aaattgaatg
      721 taaaaagggg agagaccact gaagataaaa tgtttaccct ggagacggtg aattgtctgg
      781 gttgttgtgc cattggacca gtgatggtgg tggatggcga atacaacggt cagaccacca
      841 cagctaaagc gaattctatt ctggaaaaat ataaatcccg ataaagtatc ccgaaggaaa
      901 gtgtaacgca attcaggtaa atgcagaaag gtctcatttt tcacagtgga cgcgactgat
      961 accatttaag atgcaaagga aattaacatt tggataagga aaccaaacag atgcaaaagg
     1021 tttaaaagat gagaaaacta aaaaacataa aagatttaga cgaattaaaa gaagggttga
     1081 gtaaacaaaa acctacggat aaaccgtgta ttattatttc atccggtacc tgcggccaag
     1141 cccgaggctc agccaaggtg gtggaagcgt ttgagcagaa gatccgatca aacggcttca
     1201 aagacaaggt ggctatacgg gtgactggct gtcatggatt ctgcgaggtg gagcccacgg
     1261 ttctggttta tcctgaaggg attctatatc aaaaagtgaa gccggaagat gccgaccaga
     1321 taatctccga aacgataatc aaccacaaag taatagatcg gctgctatat tcagatccgg
     1381 tgacccagca gaagttcatc catgaagaag agttcccatt ttaccgtaag cagaatcggt
     1441 tagtaatggg aaacaatagg tttgttgatc ctactaaaat tgaggattac ttggctgtgg
     1501 gtggctattc tggtcttcgt aagattttgt ctggtatgac atcagagcag gtgatttttg
     1561 aggtgaaaaa atctggtttg aggggaaggg ggggaggagg ttttcccact ggactcaaat
     1621 gggaattgtg caaaaaagcc aaaggtcaga taaaatacat aatatgcaat gcggatgaag
     1681 gagaccccgg ggcctatatg gatcgaagcc ttttggaggg gaacccgcat tctgttatgg
     1741 aagggatgtt gattggagct tttgccatcg gagccaaaga agggtatatc tacgtcagaa
     1801 atgaataccc attggcggtt aagaatgtgg gcatagccat aaatcaagca aaagaatatg
     1861 ggcttttagg tgaggatata ttagactcag gttttgattt tgatctgaag attgtaagag
     1921 gcgcgggcgc ttttgtgtgt ggggaagaaa ccgcccttat tgcttctatc gaggggagat
     1981 taggtgagcc taaacagaga cctccttatc cggtgcaaaa aggattgtgg ggaaaaccga
     2041 ccaacataaa caacgtagaa acctgggcaa atgtccccct gattattgac cgcggtgcag
     2101 agtggtattc caagatcggc acggaaaaga gcaaaggtac caagatattt tctctggtgg
     2161 gtaagataaa taatactggg ttggtcgaag ttccaatggg cattaccctt agggagatta
     2221 ttgaagatat agggggtgga atccccgggg gcagaaaatt caaggcggtt caaactggcg
     2281 gtccctccgg tggctgtatg cctgccaagc ttttggatct gccagtggac tatgaaaagc
     2341 tcaccggggt aggttccatg atgggctctg gtgggatggt ggtaatggac gaagatacct
     2401 gcatggtgga tgtagccaaa tatttcctca acttcctgaa agatgaatca tgtggcaaat
     2461 gtttcacctg tcgggagggg atcataagaa tgctagagat cgtgacggat ataactgatg
     2521 ggaaaggcac gatggcagat attcaccttc tagaagaact agcaaaggcg gttaaggata
     2581 gcactatgtg tggtctggga caaacctcac ctaacccggt cttaagcact ttgaaatact
     2641 tccgagatga atacgaagcc catgtcaaat acaagagatg tccggcagtg gtttgtaaga
     2701 aaatcatctc atctccctgc caacacactt gtcccatcaa ccaacaggga tgcgtatata
     2761 tagctttaat agcccgaggc gaatttgcaa aggcaataga catcatcaga aaggataatc
     2821 ctctgccttc ggtctgtgcc agggtgtgtc atcatccctg tgaatccaag tgccgagcag
     2881 gagaatccgg cgagcccatc tccatccgcg ctctgaaaag atttgccatc gattatggtc
     2941 tggccaacgg gataaaaccg aagaataagt tccaaaaaac cagagaagag aaagttgcca
     3001 taataggctc tggaccggct ggtttgacct gtgggtttta tttggctcaa aagggatatg
     3061 aggttacgat ttatgaggaa gcaccggtag caggaggtat gttggcgttg ggtattccag
     3121 agcaccggct ccccaggaaa attctcaacg ccgacatcga agccattaaa actgcgggtg
     3181 taatcataaa aaccaatcaa acattgggca aggatttctc cttggatgag cttttcaaga
     3241 aagggtataa ggcggtattc atcgccaccg gtgctcataa gagttggaaa ttaggaattc
     3301 cgggggaaga aggggaaggg gttttggaat caatgaaatt cctggttgca gtcaatttgg
     3361 ggaaagaggt caaaatcgga aaaagagtag ggatcatcgg tggtggaaat gctgcggtcg
     3421 attctgcgag agtggcgaat cggttttccg gctgtgagag agtaaccatc atctatcgac
     3481 gaaccaaaac cgagatgccc gctttcaaag aaagaggagg taaaaaagag gttgaggatg
     3541 ctttagaaga ggggatagac atccaattat tgaccgcccc ggtgaaagtt ttaagagaga
     3601 acggtaaatt ggttggcatt gaatgcatcc gtatgaagat gggagaaccg gatgaaagcg
     3661 ggcgcagaag acccgttccc attcccggat ctgagtttaa aatcgaattg gatactttga
     3721 ttttggccat tggggaacaa ccggacattt cctttctgac cgaaaactcc ggtgtgggta
     3781 tttccaagag tggaaccatc gtggttgatc cggaaacctt aaccactaat aaagaagggg
     3841 tattcgcggg aggggatgtg gtatctggtg ccaatacggt ggtggaggct atggcagcag
     3901 gcaaagtcgc atctgaatcg attgataagt atttgagagg agaaagcttg accagggtat
     3961 atgaggtgac gaaaccatca atcttggttg aagctttgga attgaccgaa caggagttgg
     4021 ctgagttgaa acgaccagaa atgcccgctc tatctgtgaa gcaaagacac aaaagctttc
     4081 aagaggttga attaggtttt gacgaaaaga tggcaataaa agaagccaag cgttgcttgc
     4141 gatgtgagtt agaatcagaa gaaattgagt agctgcaact cttaagaata aaaggaaaag
     4201 atatgttgag cttaaaactg aatggagtag aagttcaggc agaggaggga tggaccttac
     4261 ttgaaactgc ccggttctat gggcttccta taccaaccct gtgctatcat gaagggttgt
     4321 ccccttatgg agcttgtcga ctttgtgtgg tggagatagg agaaggtcca acctctaagt
     4381 tagtttcatc ctgcacctat ccggcagagg aggggttagt agtcagaacc cattccaaaa
     4441 gggtggaaca agctcgaaaa atggtggtgg agctactatt ggctacctgt ccggattcac
     4501 gaaccatcca ggatttagcg gcttcgatgg aagttcaaag gacccgattt aaagttaggc
     4561 atgatgattg cattttgtgt gggttgtgtg taaggatgtg caccgagcag atgcaaggga
     4621 aagccatagg ctttgtcaat cgtggtgagt tgcgcaagat aaccacacct tttgacattc
     4681 gttccgagga ttgtcgtctg tgtggaggat gcatgtatat ttgtccagct tgtcaacttc
     4741 gttgtcaggg acctgatgct ccgggagtgg tttgtggctc gtgtttgaat gtttccccgg
     4801 tctgtctaga agaacacgaa gatgccttgt gttacttaga cccctgctgt caatgcttaa
     4861 aggaagaggt taagaaataa ttcgatgagt gtttcattaa tgatatgtca attcgcaaac
     4921 aaataaatat caaataaaag gagtgaaagg ttatgtcgct aaccagactt aatgccactg
     4981 ccgccacatt gactaagaac cgaactgagg aatccgtcag tcatttcagc gggatgtgcg
     5041 tcacctgcgt ggatggctgt atcggaatgt gtgaaatcgg taaatccgcc taccgcggag
     5101 cagaggtgat ctatcctcag ccgtttggca tcatcactgc tgcttccgaa aagaactatc
     5161 cggtggatct atctcatttt acaattatgg gaactgccgt gggagctaag ggaatagcgg
     5221 aagattccaa tgtggctatt ttccccaatg cgaatctgga aagaaaaatc ggaaacgatc
     5281 ccaagattaa gcttagactc ccctttatta ttcctgggct ggggtcaacc aacatcgcca
     5341 agaataactg ggagggtttg gccatcggtg ccgctctggc tggaattcct ttaaccattg
     5401 gagaaaatgt ctgtgggatg gacgtggaat cagtgataga aaacggaaaa gtgaaacatg
     5461 ctccagatct cgcctggaga gtgaagctgt acaaggattg gcaacaagat ggctatggtg
     5521 ctatcgtgct tcaagccaac attgaggata ccgccctagg ggttttggaa tatgggctca
     5581 ctaagttggg tgtggatgta gtggagctca aatgggggca aggagccaaa gacatcggtg
     5641 gagaggtgaa aatcagttcc cttcagaaag cacagctttt gaaaaagaga ggatatgtgg
     5701 ttttgcccga ccctctggat ccagccaata ttgatgcttt cgaaaaagga agtttcaagg
     5761 agtttgagag gcattcgcga gttggaatgg ttactgagga aggcttcatg aagagagtga
     5821 aagagttgag agacgctgga gccaagcaca ttttccttaa gaccggagct taccgaccag
     5881 cagatttagc cagggcaatc aagttctcct ccaaagccag acttgatctt ctgaccgtgg
     5941 atggtgctgg tggtggaacc gggatgagtc cttggaggat gatgaatgaa tggggaatcc
     6001 cttcagtaga actccattct cttttgtatc aatatgcgaa taagttggcc aagaagggtg
     6061 aatatgtccc ggacatcgct atcgcgggtg gattcacctt tgaagatcaa atatttaagg
     6121 ggttagcctt aggtgctcca tatttcaagc tgattggtat ggcacgagga cccctcgccg
     6181 ccgccatggt gggaaaaacc attggtaaaa gaatcaacga aggaatggtg ccggtattta
     6241 ttgagagatt tggcaatacg ccagaggaaa tttttgtctc tgctccggaa ttaaaacgga
     6301 aattaggcga agccaccttc aggaaagtcc caactggcgc attgggttta tatacctata
     6361 tgcagagatt agcccaagga ttgagacagc ttatgtctgg caatcgaaag ttcgctttgg
     6421 aatatatcac cagagacgac attgcggctt tgactaagga agcttcagag atcagcggaa
     6481 tatcttatgt gatggatgcg gacaaggagg aagcagaaaa gatattaaag agctgaggct
     6541 tctagttcaa aagccgaata gaacgctgat tcactcaaca tacttaatac ttttagaagc
     6601 tttcgggaga agggaactct cgaaagcttt ttgttggtat cggtatttcg cgatgcggat
     6661 gtagtatgga ataactcaat tcctttgtcc tggtatttgg agaaagagta gaggacttta
     6721 cccataatct ttagatttta tcctcatttt gacagggttg aagatttctc taatctccaa
     6781 cccttttgtt ttttttagca tgatctttgt aaaatgatac tcaagaaagg tgatatgctt
     6841 attttccctt gacaattcta aagggagtgc ttaatatctt aggtttatga gacttgaact
     6901 tagcaagctg aagcaggaaa aggttaattt tcagttcacg gagagcgccg aagagctgga
     6961 tttaagggca gacggagtgg agtttggttc tccgctcaag gtcaaagtgg aggtttcacc
     7021 cagcggggag agttttctgg cctgggctaa aattgagacc agggttaaac tggagtgtgt
     7081 ccgttgcctt ttaatttact catatcctct ccaggctgaa ttcaatctac ttatagagaa
     7141 acaaagaccc ggacttttgc ttgatccggg gaatgaggat acagtggtaa taaatccaca
     7201 ggacaagttt attgacataa gcgataggat aagacaagcc atagccttga gcttgccatt
     7261 gaagtctcta tgtagtccgg actgcaaagg gctttgtgac atttgcgggg cggaccttaa
     7321 cacttctcca tgcagctgca ccatagagga gtatgacagc cgctgggata aattgaaaca
     7381 agctttgaaa aaggaaaggg actgatatgc cgttacctaa aagaagacat tcgaagacgc
     7441 gtggtgccaa gaggagaact cactggaagc tcggctctcc taatgtgata gagtgccctc
     7501 attgtcatca acccaaattg tcacacaggg tttgtcctca ttgtggctat tataaagaga
     7561 aagagataat cattccagga aagagttgat tcggagaaat ggcagataag gtgagaatag
     7621 gtttagatgc gatgggcggg gataaggcac cggcgagtat agttgaagga gcgctcttag
     7681 ccagtcgcga caggaaagct gattaccaag tggtcttggt gggagataag gggaaaatcc
     7741 agagagagct ggaaagactg ggcgtctctg ttcctccatt tgaggtagta cacgcttccg
     7801 aggtggtcag catggatgag ccggcagctg aagcggcgcg taagaaaaaa gactcttctc
     7861 tggcagtggg tatggggatg caaaaggagg gtaaactaga tggattcgtg tcaggtggaa
     7921 ataccggtgc agttatggct acagcagtcc taactctggg acatctggaa ggggttgacc
     7981 gtccagccat cgcctcatat tttccttcgg agaaagatgc ggtggtggtt ttagatgtgg
     8041 gagctaatgc cgactgcaag gctcgtaatt tgtatgaatt tggagttatg ggaacaattt
     8101 acaccaacta tatcttaaaa agggagaatc cgagattagg gcttctctcc atcggagagg
     8161 aaagcaccaa gggaaacgaa ttgactttag tgtcttatag gttgctcagc gagagcaagc
     8221 tgaattttat aggaaatgtg gaaggacatg atgtactcaa aggcacttgc gacgtagtgg
     8281 tgtgtgatgg tttcgtagga aatattatgc ttaaattcgc tgaaagcata gaaagtttcc
     8341 tttatttctt gataaaagag gaactgcgta gaagcatact tttcaggacc ggcgcctttc
     8401 ttttgaaata tcccctccgc agtctgagaa aatctttgaa ctatgctgaa tatggggggg
     8461 ctccgctttt gggagtggat ggcgtatgta tcatctgtca tggcaagtca tcccccaaag
     8521 ccattaaaaa cgcagtcaaa ttggcctcca agatggtaaa tgaaaaagta aacgaacaca
     8581 tcaaggagga attaagaaac agttatcagc tggaaggaaa aaatggcaaa tcgttataat
     8641 gcaaggatag ttggaaccgg catggctgtc cccaaaagag ttctaaccaa tgccgagtta
     8701 gagaagatga tggatacctc ggatgaatgg ataaccgcac gcagcggaat aaaggaaaga
     8761 aggatagcag atgaaaacac tgccgcttcg gatttggctt tggaggcatc cttgaaagca
     8821 atggaagagg cgaagcttaa acccgaagat cttgacctga taatagttgg aacagtcact
     8881 ccggatttta cgcttccttc cactgcctgt attcttcagg ataggttagg caacaaaacc
     8941 gcaggggtaa tggatgtggt tgcagcttgc agtggattca tctatggctt atccgtggct
     9001 aaggctttcg ttaacatagg acaatttaag aacgttttag ttataggagt ggagatattg
     9061 tccaaattta ccaactggga tgacaggaac acctgtgttc tttttgggga cggggctggt
     9121 gcagccattg tccaggcatc cgaggaagag ggaggaatta tctccaccta tatttccgga
     9181 gatggttcat tggcaaacct tctgcatgct cccattggag gaacgcgcac tcgactaacc
     9241 aaggagaatt tcgacttaaa acagcataac atcataatgg caggaagtga ggtcttcaag
     9301 tccgcagtcc gggctatggg cgattctgct gtaaaggtga ttgaacagtc cggcttaaag
     9361 ccagaggata ttgaccttct tattccacac caagccaaca tccgaatcat cgaagccaca
     9421 gctagaagac tgaagatgcc tatggataaa gtatatgtca acatagataa gtatggcaac
     9481 acttcatccg cctctgtgcc aatagcgtta gacgaagcca ggagaaaagg caaaatcaag
     9541 aagggggata aaacagtatt ggttgctttt ggtggtgggt tcacttgggg tgcggcttta
     9601 attcagtggt gaaacttgtg aaaaattttg caggcatttt tccaggacag ggctcgcaat
     9661 atgtaggaat ggcaagggat ttatatgatg cctatctgca cataaaggaa ttctacaaga
     9721 aggcagacga tgttttagga tatggcttgt ccagagtttg ttttgaaggt ccagaaaaga
     9781 ctttaacaga aaccaaatat actcagccag ctatcttcgt gcacagtctt gccttgtgga
     9841 atctaatgtc cgaaagaaaa aagataaggc ccagtttcgt cgcaggtcac agcttggggg
     9901 aatattctgc tttggtggtg gcgggtgttt tctcatttga agatggtctt tttgcagtca
     9961 aacagagggc ttctttgatg caggaagcgt gcgacaggaa caaggggaca atggcggcta
    10021 taattgggct ttcacgggag gatgttttag agatttgtaa ggaagcttca tccaagggaa
    10081 taattcagcc agccaatttt aattctgaag agcagattgc catttctgga gaaatggaag
    10141 ctgtcgaatt cggagtggaa ttggctcaaa ggaaaggtgc caaaagagcg atgcttcttc
    10201 aggtgggagg tgcctttcac tctcccctga tgaattcagc cagagagggt atgtccgaag
    10261 ttttgagcaa ggtcgagata aaccccgctc gaattccagt ggtgtccaat gttactgccc
    10321 gggtgatgac ccagccgcag gagattaagg atgctttggt agagcagata accaagccgg
    10381 tgctgtggta tgattccatg caatacgtct atcaacaagg agtgagagat tttgtggaaa
    10441 ttggcccggg aaaagtcctg caggggataa taaaaaggtc ctttaacgac gcttcgaact
    10501 ttggtgttga taaatcagtt gatttagata aattcttaga attattggaa atcgcataat
    10561 tcagtgacca agcagttcat tttccttctc ccttgaggga gaaggttagg atgagggtgg
    10621 gatagagttt acaccccctc acctttgtcc tctcccccaa ggggagagga agaaaaatta
    10681 tcaatctaat ttacgaaact caatagtatg aatctaaaag acaaaaccgc aatcgtaacc
    10741 ggtggagctc aaggcatagg caaagccata tgtgaaaggt tggctgggga ggggacgaaa
    10801 atagcggtct tcgatatcga cctggattca gctacgtcct tagctcaaag tataaaagac
    10861 agaggtggct tagcccaggc tttaaagatc gatgtttcca gtttccagga agttgaacag
    10921 ggggttaata aggtgattga acagttttcc acaattgata ttttggtcaa caatgcaggc
    10981 atcaccaagg acaatctgtt tattaaaatg ttcgaggagg aatgggatag ggttattcag
    11041 ataaacctga aaggagcttt taatctttgc aaaagcgttg ctcgaattat gctgaaacag
    11101 aagtcgggca agataataaa tatttcttca gtaataggga taatgggaaa tgtcggtcag
    11161 gcgaattact ccgcatctaa agcaggttta ttgggcttaa ctaaatctct ggctaaggag
    11221 ctagcctcgc gcggaataac tgtgaatgcg gttgccccgg gatatatcca gactcagatg
    11281 accgagagtc tatcgcaagc ggctagggat gcatttttaa actcgattcc cttaagaaga
    11341 gcaggtcagc cagaagaggt agctaatctg gttgcctttt tggcttcgga agaagcgaat
    11401 tacatcaccg gtcaggtgtt ccagatagat ggtggattat tgatgtagag aacaaaacaa
    11461 tcttcaacca aaaaggagga gtgcatttta tgtccgttga acagagggtt aaggagatca
    11521 tagtcgaaca gttgggagtt gacccggaac aggtcaccaa cgaggcatca tttgttgatg
    11581 acttgggtgc tgattccctg gatactgtgg aattggtaat ggctttggaa gaggagtttg
    11641 gcttggagat accagatgaa gaggcggaga agataaccac tgtgggagaa gctatagagt
    11701 atatagaaaa gaatgcagta gaggggaatt agggtagttt ctttagatcg ataattctgt
    11761 tgtcttgagg tagcagatga aaaacagagt tgtggttacg ggaatgggtg ccatcacgcc
    11821 ggtggggaat agcgtggatg tattctggga agcgctgaag aatggtgttt ccggtgtgga
    11881 taaggtaagc cgatttgatg tggctaacta tcccaccaag atcgcagccg agattaaggg
    11941 gtttgatacc tctgcccaaa ttgataagaa ggaagcgcga cgaatggatc tatatgagca
    12001 gtacgctatt gtagccagcc agcaggtagt ggacgacgct gggttagatt taaactcgat
    12061 ggataaaagt cgagctgggg tagtgattgg ttccggcata ggagggatag agacctttga
    12121 gaaacagtgc agtacaatga taagctccgg cccagggaga gtaagtccct tcttcattcc
    12181 tatgatgata attgatatgt gcgctggttt ggtctctata cgtttcggat ttaccgggcc
    12241 aaactatgcc acggtttcag cctgtgcctc cagcgctcac gctgtggcgg atgcttttag
    12301 aattatccag cggggggaag cggaggtaat gataaccggg ggaagtgaag ccgctattac
    12361 tccactctct tttgcgggtt tctgctctgc cagagcgatg tcaactcgca atgacgagcc
    12421 cgagaaagcc tctcggccgt tcgataagca aagagatggt tttgtgatgg gtgagggtgc
    12481 gggcatgctt attttggaaa gcttggagca tgctcaaaaa agaggtgcca aaatttatgc
    12541 ggagattgca ggagtgggaa tgagtgcaga tgcttatcac ataactgcac cttcgcccgg
    12601 aggtgaaggt gcagttagat ctatgcaagc tgctattcaa gatgccggcg tttctccgca
    12661 agaggtgggt tacattaatt cgcatggcac tgccacagat ctgggagata taagcgagac
    12721 tgtggcaata aagaaagtct ttggagaaca cgccaaaaag atcgccataa attccaccaa
    12781 gtcaatgacc gggcaccttc tgggagcagc tggtgcagtg gagctgattg tcggaataaa
    12841 atcgatgtca gagggggtca ttcatcccac aataaacctg gagtttccgg acccggagtg
    12901 cgacttggat tacgtgccca atctcaaaag ggaaaaggaa ataaacgttt tcttgagtaa
    12961 ctcctttgga ttcggcgggc acaacgtcac cttggtggtt agaaaatttg taccatagat
    13021 aacttttttt agaaagtcgg gaagcgtatt tattcttccc gacttttttc taatatgagc
    13081 cctttcaggt atatcaagaa gatattctcc aagagctccc aattggggaa gctggagaag
    13141 atgaccggat atcacttcaa caacttaaat ctccttaacc aagccttaat tcaccgctcc
    13201 ataataaagg acaaatccac tcaagtccta caaccgaatg aaaggctgga gtttttggga
    13261 gatgcagtat tgggattggt agtgagccgt tttttatatg agaagtttcc ggaaaaaaga
    13321 gagggggatt tgactaagct caaggcggtt ctggtgagcg aatcaacctt gcgccgcatc
    13381 gccaaggaca taaatctggg tcagttcctt caattgagcg aagaagaaaa caaagccgga
    13441 ggcagagaga gaagttccat actggcagat gcgtacgagg cgttgatcgg agcaactttt
    13501 ttggatggtg gactggctcc ggctagaaaa atgataagaa accaactctt aaagaaatac
    13561 tgggagttga ccacagatga agaacatttc aattataaag gtgagctttt ggagtatttg
    13621 cagtctttcg gttggggtat gcccagatac gagatagtgg aggagaaggg accggaccat
    13681 caaaagaaat tcaccataga tgtctttgcc cagggaaaga ggatgggtag gggaaccggc
    13741 accagcaaga aggaggcgga acaaaaagca gccaagaaga ctttggagaa tataaataag
    13801 ggagaacttt taaataggca agtgaatgga ttatctcctg actgaagcac aaaaaaaact
    13861 aaaacaacaa attagaaaaa taaccctaaa cccagatttt gaattagcaa aatctgcctc
    13921 ccgagctcct cttcttgcat tctgcaagaa taagagagga gccgggacta aatcctcgtc
    13981 ccgccagggt gggacagagg gggcaagaat gccgaagtta tcccaaaatt tgctcgcact
    14041 tttttgtatt gcattgcaat agagcaacca ttccggttga aaagatgatg tgtgatgcca
    14101 agatcgctca aatctacaaa gggacaaatg agattcaaag ggggatcatt gctttgaatt
    14161 tgataaaaga gatggcagaa aaaaagaaga agtaagaggc aaatggcgat tagtaggaga
    14221 atgggctgtt cgctgagatg tgatttgtgc aaggtccctg aaaagctgtc taggtaagga
    14281 gaagcacgaa ttctgtgtcc tgagattgga gtgtctgcct tccttcggca agctcaggac
    14341 ggtgagcaaa gtcgaacccg ttaggcagac ccccggttcg gctaaagccg aacactccag
    14401 agcacgggct tgatgaatcg aacccctacg cgatcagtta ttgtcgggtc agcctgcccg
    14461 tttctccccg ctcgacccaa cggccagggg ggacccgaca gacacaataa cctgcacagg
    14521 gataaattcc ctgtgctacg aatataacat atcgtaggtg tgggaatttc cttcgacctt
    14581 gctcaggcct tcggctctga ggctcaggct cgaagagatg gtgaacttgt tgaaccatat
    14641 cccacaccgt gtatgtttct ttaatgaaaa cttctttttg gcgcttccta tacactccgc
    14701 aaaaaaatgc ttttttcaat atggcaacag atgaaaccct gacttatctt tgccaagagg
    14761 aaaattgcat ccccacactt agactctata cctggtctcc tccagcagtc tctctggggt
    14821 attttcaaaa gatagaatca gctgtaaata tggatgaatg tataaagtta ggcatagacg
    14881 tagtcagaag gccaactggc ggaagagcgg ttttgcatca ggacgagttg acttacagtg
    14941 tttgtggctc aggcgagaat ttccccgaat tgggaacaac cgttagccag acctacgaaa
    15001 ggatcagtct agcattactg gagacgttaa gaatcctcgg aataaaagga gaatgggtaa
    15061 gatcacttcc cagacaaaag aaggaaaaaa tatccgagcc ctgttttgtc agttcctcca
    15121 agtatgaaat aaccgtggac aacaagaaac tgataggaag cgcacagaga agatttggta
    15181 aggcttttct gcagcacggg tcaattcccc taaagagaaa taataagagc ttggctcact
    15241 ttctgccaca gagaaaaagc gtggatgcaa tggataagtt gctaacaact cgctcaacca
    15301 ccattgagga gcttttgaat cgagaagtaa agattgagag cataatagaa gcaattaagt
    15361 ccggcttcaa agggtatttt gaaattgaat tcatggaaga gaacttgaca gataatgaac
    15421 tcgatagaat tgagaagtta ataagcgaaa aatactacac taatcaaact aatcccgcaa
    15481 aataatgcat ggaaggtcgc agaaaattgc ggatgggtaa tattctgttt aataaagagt
    15541 tatagcaaca atcccggatt taactggtta ttcgtcgcag aggattgcgt ttttgcttac
    15601 ttggtatatg tgagcaactc tgcagtgcaa atttaagttg ctgtttggca atgaattgaa
    15661 gaaattacaa aaatagaggg cggggctgaa atttggcaca gattttgttg agattttaat
    15721 tcggtgtttt ctttcttctt atggtttgga agcgttcttg aaaaacctgc ctgcgtttta
    15781 atactctaat actattgacg caggcaggga gaacaaaaat tgtttgataa ttttacttgt
    15841 gtaacattaa gaaaactctt gacatctcaa tggttagtta gttaattaac atttactttg
    15901 aacgcaaaaa gtaaaagcaa attaactttc accttttacc aaatgccaag gaggtaagtt
    15961 gccatgaggt gctacaaaaa cgccaatatt gtggcaattg tttttttggg gattttttct
    16021 cttatattcc tgagtgtttc cagctacgcc ggtgtctccg gaaaaatcag cggcaaagta
    16081 attgacaaaa acagcaagat gcctctgccc ggagcttcta ttcagatagg gggaaccagt
    16141 atggggaact actccgggcc agatggttcc tacttcatac tcaacgtcgc tcccggaacc
    16201 tacgatgtca cggcaaagct cattggatat aggatagtgg aagtaaaggg cgttaaggtt
    16261 gccactgaca aaaccactga acaggatttt gagcttaccg tctccgcggt ggagttaaaa
    16321 gaagtggttg aggtaatagg tagggcggag gtaattgaaa aggacgtcac caccaacgtg
    16381 agaactgtat cctctgacca gattcagaat atggcggtca aagaggtagc ggatattctc
    16441 aaaacgcagg tcggattcgt aaccaagaac atggagcttc atattcgcgg tggtcgtgcc
    16501 ggggaagtcc tttacatcgt ggacggagtt gagaccaaag atcctttagg tggattggga
    16561 atagtgcggg ggggtatgga ggtttcagct gacaacattg aagagatttc ggttttgaaa
    16621 ggcggattcg atgctgaata tggcaacgtg cagtctgcgg tgatcaactt ggtaaccaaa
    16681 gagggaagta tccagaagac cactggtcac gttgagtttc tgacggatga ttttggggat
    16741 aagagattaa ataagtattc gttcaattcg gaccgcatag aaatgagtct gagcggtccc
    16801 gagccgattc tttcgcgctt cctcgttccc ctaactgaga aattctctcg tgagaagtta
    16861 gcttatagat taggattgga tgcttataaa acggacactt attacgatat taataaatat
    16921 tctactccga gtgccgaaag gaagtttgta tcttataata tcttggggat tgatgtgccg
    16981 gatag
//
LOCUS       DASWLN010000161         8477 bp    DNA     linear   ENV 20-FEB-2024
DEFINITION  MAG TPA_asm: Candidatus Zixiibacteriota bacterium isolate
            SMAG_U2949 k141_208615, whole genome shotgun sequence.
ACCESSION   DASWLN010000161 DASWLN010000000
VERSION     DASWLN010000161.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37960594
            Sequence Read Archive: SRR10376091
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Candidatus Zixiibacteriota bacterium (soil metagenome)
  ORGANISM  Candidatus Zixiibacteriota bacterium
            Bacteria.
REFERENCE   1  (bases 1 to 8477)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 8477)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (26-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/12/2024 08:28:26
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 1,440
            CDSs (total)                      :: 1,416
            Genes (coding)                    :: 1,412
            CDSs (with protein)               :: 1,412
            Genes (RNA)                       :: 24
            rRNAs                             :: 1 (16S)
            partial rRNAs                     :: 1 (16S)
            tRNAs                             :: 21
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 4
            CDSs (without protein)            :: 4
            Pseudo Genes (ambiguous residues) :: 0 of 4
            Pseudo Genes (frameshifted)       :: 0 of 4
            Pseudo Genes (incomplete)         :: 3 of 4
            Pseudo Genes (internal stop)      :: 1 of 4
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..8477
                     /organism="Candidatus Zixiibacteriota bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_208615"
                     /isolate="SMAG_U2949"
                     /isolation_source="soil"
                     /db_xref="taxon:2053527"
                     /environmental_sample
                     /country="USA: Oregon"
                     /lat_lon="42.99 N 123.63 W"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            complement(<1..810)
                     /locus_tag="VGB16_05290"
     CDS             complement(<1..810)
                     /locus_tag="VGB16_05290"
                     /inference="COORDINATES: protein motif:HMM:NF024867.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016887 - ATP hydrolysis activity
                     [Evidence IEA];
                     GO_process: GO:0006302 - double-strand break repair
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="AAA family ATPase"
                     /protein_id="HEX9917133.1"
                     /translation="MLKALRIENFALVDRLEIEFGSGLNILTGATGAGKSIIVGAINL
                     VLGERASPEMVRTGTAVASIEASFKTNTDNPIFNMLKALDIATSSNTLSIRREISSKG
                     ISRCFINDRLVTLANLKSVGDKLGDLHGQHQHQALLDTNKHIDYLDSFGNLKEEVDTI
                     GETFKSLRIRSEELRRLKEQERADGERKELYCFQSNEISRANLVLDEEGKLLQQKKVL
                     ENAEELFGLVSSVSAGLYEEDDSAAEKLSFYLDELKKATELDSNLKEELENL"
     gene            complement(838..1704)
                     /locus_tag="VGB16_05295"
     CDS             complement(838..1704)
                     /locus_tag="VGB16_05295"
                     /inference="COORDINATES: protein motif:HMM:NF039482.3"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003951 - NAD+ kinase activity [Evidence
                     IEA];
                     GO_process: GO:0006741 - NADP biosynthetic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="NAD(+)/NADH kinase"
                     /protein_id="HEX9917134.1"
                     /translation="MKSLGIIANVNKESMPEVVQKIIQWCSKTKIDFFLSEEVASLLG
                     HKGKSFAREELWEHSEIVVSLGGDGTMLAAARAVGEHQTPILGINVGSLGFLTEITAN
                     HLTTTLDLIKAGDFYIEKRMVLAGEVEGVDSKSLFALNDLVVDKGGVARLLQFHLYDN
                     EELVCSYSADGLIISTPTGSTAYSLASGGPVINPTMNAIIVTPICPHTLAIRSIIFSE
                     NEKLKVVVEREQHAPTLTADGQVAVTLKPGAVIKIGKAEHTVNLIKFKGKSFYDILRT
                     KLHWGVKPKGDQ"
     gene            complement(1708..3612)
                     /gene="dxs"
                     /locus_tag="VGB16_05300"
     CDS             complement(1708..3612)
                     /gene="dxs"
                     /locus_tag="VGB16_05300"
                     /EC_number="2.2.1.7"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_014856973.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0005737 - cytoplasm [Evidence IEA];
                     GO_function: GO:0008661 - 1-deoxy-D-xylulose-5-phosphate
                     synthase activity [Evidence IEA];
                     GO_process: GO:0008615 - pyridoxine biosynthetic process
                     [Evidence IEA];
                     GO_process: GO:0009228 - thiamine biosynthetic process
                     [Evidence IEA];
                     GO_process: GO:0009240 - isopentenyl diphosphate
                     biosynthetic process [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="1-deoxy-D-xylulose-5-phosphate synthase"
                     /protein_id="HEX9917135.1"
                     /translation="MAKLLDSINSPQDLKKIPLEDLPKLAAEIREEIITVVSKNGGHL
                     ASNLGAIELSIALHYVLDSPRDKIVWDVGHQCYTHKLITGRREKFSTLRKYKGISGFP
                     KKKESEHDAYEVGHASTSISAALGMACARDYLNQNFHIAVVVGDGALTGGLSFEGMNN
                     SGALSKNMLVVLNDNTMSISKNVGALSKYLTNILTDEKYNKIKAEVWDLVGKFKRRDK
                     IRTMVAKVEEHVKGFLVPGIIFEKLGFRYFGPIDGHDIQQLVRILEHIKNLSGPVLLH
                     VLTQKGKGYKYAEEDAPRFHGIGVFDKVTGNSDTQPGQLSYTDVFGQAIVELAEKDEK
                     IVAITAAMSLGTGLVDFAKKFPNRFYDVGIAEQHGTTFACGLATLGMKPVFAVYSTFL
                     QRAFDQVLHDTALQNLPVIFAVDRAGLVGEDGPTHHGAFDLSYLRQIPNLTIMAPKDG
                     NELRGMLNFAVSWNKGPVVIRYPRGTMPDEKIEQGFKEIKMASWEVLRSGEDILVLAV
                     GSMVYPSLEAAQICAENGIDVCVVNARFVKPLDENLLSSFLRKFNRIITVEENALAGG
                     FGSAILEYIEAQGIRKKISVKRMGIPDSFIQHGSRKTLLEELHLDRDSIVQLIKQTFT
                     FRKKKVIAET"
     gene            complement(3641..4501)
                     /locus_tag="VGB16_05305"
     CDS             complement(3641..4501)
                     /locus_tag="VGB16_05305"
                     /EC_number="2.5.1.10"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_012529688.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004337 - geranyltranstransferase activity
                     [Evidence IEA];
                     GO_process: GO:0008299 - isoprenoid biosynthetic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="farnesyl diphosphate synthase"
                     /protein_id="HEX9917136.1"
                     /translation="MKPEDYLRNKKRIIDNALDRYLPSSHKPPRKLHQAMRYSVFAGG
                     KRIRPILAMAAYELVGGKGNSILPVACALELIHTYSLIHDDLPCMDDDDLRRGKPTCH
                     RVFGYGMAVLAGDALHALAFELLAKSDNPKIVVEVANSIGTVGMVGGQAADLEAEGKK
                     VNLKEVNFIHIHKTAALLRACIRVGAILGKANSKQLKALTSYGNKFGLAFQITDDILD
                     LEGEERKLGKRVGVDKDKAKATYPKVIGVERSKQEAKRLVQSAKKDLKSFKKTGILKQ
                     LADFLISRTN"
     gene            complement(4502..4738)
                     /gene="xseB"
                     /locus_tag="VGB16_05310"
     CDS             complement(4502..4738)
                     /gene="xseB"
                     /locus_tag="VGB16_05310"
                     /EC_number="3.1.11.6"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011736267.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0009318 - exodeoxyribonuclease VII
                     complex [Evidence IEA];
                     GO_function: GO:0008855 - exodeoxyribonuclease VII
                     activity [Evidence IEA];
                     GO_process: GO:0006308 - DNA catabolic process [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="exodeoxyribonuclease VII small subunit"
                     /protein_id="HEX9917137.1"
                     /translation="MPKKMNFEKGLSRLEEIVEQLESGDLALDQSLKIFQEGIGLFRF
                     CSQKLNDAQSKVEKLVELSDGKFKLEPFDFKEEE"
     gene            complement(4742..5944)
                     /gene="xseA"
                     /locus_tag="VGB16_05315"
     CDS             complement(4742..5944)
                     /gene="xseA"
                     /locus_tag="VGB16_05315"
                     /EC_number="3.1.11.6"
                     /inference="COORDINATES: protein motif:HMM:TIGR00237.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0009318 - exodeoxyribonuclease VII
                     complex [Evidence IEA];
                     GO_function: GO:0008855 - exodeoxyribonuclease VII
                     activity [Evidence IEA];
                     GO_process: GO:0006308 - DNA catabolic process [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="exodeoxyribonuclease VII large subunit"
                     /protein_id="HEX9917138.1"
                     /translation="MPDKIYTVSEITKQIKELLEGELPTIWIEGEISNFVLHSSGHRY
                     FSLKDEEAQIRCVLWRGKGEGLDFEPQSGMKVLALGKISVYEKRGEYQLYVEILRPLG
                     IGKLELELQRLKERLQKEGLFDEEHKKAIPKFPETIGIITSPTGAAIRDIIKIIQRRA
                     PYIKLILNPVRVQSEGAEDEIARAVAQFNQYGKADLIIVGRGGGSLEDLWAFNEEIVA
                     RAIFKSKLPIISAVGHEIDFTISDLVADLRAATPSVAAEMVVRDKKELERELNSLAGR
                     IVNYQNSILGYNKNRLESIQRSYGFRKPLDLLNQKMQRTDEVWRAFLLVMKNYFELKK
                     KGLSSFFSHLNALSPLAILERGYSITRKVPGLSVVKDAARLKIDDEVEVKFHLGHIRA
                     EVKKINEE"
     gene            complement(5946..6854)
                     /locus_tag="VGB16_05320"
     CDS             complement(5946..6854)
                     /locus_tag="VGB16_05320"
                     /inference="COORDINATES: protein motif:HMM:NF025910.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="tetratricopeptide repeat protein"
                     /protein_id="HEX9917139.1"
                     /translation="MEKVKKDAYEKAIQGANSSVSWAGRVIAAISLFAIALGVFGYLQ
                     GRALIKQMEGELERAKEIKDEVQKKKEEVEGLCEEIKQMRSKAAEIVRDAEKITEEAK
                     KTKTPLDFSEEAKAIISEKDAEKTIQRYEKLTDKIFGEDAKLVYNQLAFSYYRLQRYE
                     EEISILRKTIQINPKDAIAYYNWGVALGELGKHEEVIEKCKKAIELKPDLTEAYYNWG
                     VALIKLGKYEEAIEKCKKAIEIKPDYAEAHYNLAYAFAELKDKNQMLKYLKDAINLDN
                     KYREKSKTDTDFKTVWNDPDFKKLVE"
     gene            complement(7647..>8477)
                     /locus_tag="VGB16_05325"
     CDS             complement(7647..>8477)
                     /locus_tag="VGB16_05325"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_020740068.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="FG-GAP-like repeat-containing protein"
                     /protein_id="HEX9917140.1"
                     /translation="PSTEYTVGGNNPHLIFAADLDGDGDLDLATANYGSHNVSVLLNN
                     GNATFSPSTEYPVGGNNPRSIFAADLDGDGDLDLATANEGGRTVSVLLNINIDIAVIN
                     IVPSKEIVVQGDSVSIDVTVQNQGSVRETFDVTTFYDDIPIQTKTVTELAPSQETTIS
                     FNWNTTGIVLGNYTISAYAPLLPGEIDTDDNTYRDGMVSIIYDCGDYSCGDCNGDCKI
                     TVSDVVYLINYLFKGGYVPVPEKCLGDANGDGKVTVSDVVYLINYLFKGGPAPAACCQ
                     "
BASE COUNT         2236 a         2064 c         1534 g         2643 t
ORIGIN      
        1 aagattctcc aattcttctt ttaagtttga atccaattct gttgccttct taagctcatc
       61 caaataaaaa gacaacttct ccgcagcaga gtcatcctcc tcgtaaagcc cggcgctgac
      121 agaagaaacg agtccaaata gttcctctgc attttccaaa accttttttt gctgaagcag
      181 cttgccttct tcatccaata ccaggtttgc tctgcttatc tcattcgact gaaagcagta
      241 tagttccttt ctttcgccgt cagctctttc ttgctccttt aatctcctta gctcttcgct
      301 cctgattctc agcgatttaa aggtctcacc gatagtatcc acttcttctt tcaaattccc
      361 gaagctatcc agataatcga tatgtttgtt tgtgtccaag agcgcctgat gctgatgttg
      421 accatgtaaa tcacctaact tatcgccgac tgatttgaga ttggcaagcg taaccaacct
      481 atcgtttata aaacagcgac ttatcccctt ggatgatatc tcgcgccgga tgcttaaagt
      541 gttacttgag gtagcaatat ctaacgcctt taacatatta aagataggat tatcagtatt
      601 agtcttaaaa gaggcttcta tgctcgcaac tgcagttccg gttctcacca tctccggact
      661 cgccctttct cctaaaacca gattgattgc tccgacgatg atggactttc ccgcaccggt
      721 tgctccagtc aggatattaa gccctgaacc aaactcaatc tccaacctgt caaccagagc
      781 aaaattctct atgcgcaagg cttttagcat aagcttatgg aaggaaaaat ctgtagctta
      841 ctggtcccct ttaggtttga ctccccaatg gagttttgtt cttaaaatgt cataaaatga
      901 cttaccctta aacttaatca gattaacagt gtgctcggct tttcctattt tgattactgc
      961 tccgggtttg agggtaacag ccacctgacc atctgcagtc agagtcggtg catgctgctc
     1021 cctttcgacc accactttca gcttttcgtt ttcggaaaaa atgatcgagc ggatggctaa
     1081 ggtatgagga cagatgggcg taacgataat ggcgttcatc gttggattta tcaccggtcc
     1141 tccagaagcc aaggaatagg cggtcgaacc tgtcggtgta gagattatca atccatcagc
     1201 tgaataggaa cagaccagtt cctcattatc atagaggtga aattgcaaaa gtctggcaac
     1261 tccacccttg tccactacca aatcgttcaa ggcaaaaaga gatttagaat caaccccttc
     1321 cacctcacct gccagaacca tccttttttc tatataaaaa tcaccagcct tgattaaatc
     1381 caatgttgtg gtcaggtggt tagctgtaat ctcagttaaa aaacccaggc ttcccacgtt
     1441 tatccccaaa atcggggttt gatgctctcc tactgctcga gctgcggcaa gcatagtccc
     1501 atctccaccc aaagatacaa ctatctcact atgctcccag agctcttccc tggcaaagga
     1561 tttacctttg tgaccgagta gcgatgccac ctcttctgat aagaagaaat cgattttagt
     1621 tttggaacac cattgtataa ttttctgcac aacttccggc atagattctt tattgacgtt
     1681 agctattatt ccaagacttt tcatttttta agtctccgct atgacttttt tctttctaaa
     1741 ggtgaatgtc tgtttaatca attggactat gctatctcta tccagatgca gttcctccaa
     1801 aagggttttc ctggaaccat gctggatgaa actgtccgga atccccattc ttttaaccga
     1861 gatctttttc ctgatccctt gagcttcaat gtattccaaa atagcgcttc caaaaccacc
     1921 agccagagcg ttctcctcaa ctgtgatgat tctattgaac ttccgcagaa aggaggaaag
     1981 cagattttcg tccagaggct taacgaaacg cgcattcacc acacatacgt caattccatt
     2041 ttcggcacat atttgtgccg cttccaaact gggatagacc attgagccaa cagccagtac
     2101 taaaatatcc tcccctgacc tcaatacctc ccagcttgcc atctttatct ctttaaagcc
     2161 ttgctcaatt ttttcatctg gcatggtacc acgtgggtaa cgaatgacca ccggaccttt
     2221 gttccaacta actgcaaagt ttagcatacc cctcagttcg tttccatctt taggtgccat
     2281 aatggtgaga tttggaatct gtctgagata acttaaatca aatgctccat gatgagtggg
     2341 accatcctct cccaccagcc cggctctatc cacagcaaat atgaccggca ggttctgcaa
     2401 agctgtatcg tgcaagacct gatcgaaagc cctttggaga aaagtggaat ataccgcaaa
     2461 gaccggcttc attccgaggg tagctaaacc acaggcaaag gtagtgccgt gttgctctgc
     2521 aatccccaca tcgtaaaatc tgttagggaa ctttttggcg aaatcgacca agccagtgcc
     2581 caaagacatc gccgcagtga tagctactat cttttcatcc ttttccgcca actccactat
     2641 ggcttgtcca aagacgtcgg tgtaacttaa ttgccctggc tgggtatctg agtttccggt
     2701 cactttatcg aacaccccaa tcccgtgaaa acgaggggca tcttcttcag catatttgta
     2761 acctttaccc ttttgggtca ggacgtgaag cagtactggt ccacttaagt ttttgatatg
     2821 ctccagaatt ctcactaatt gttgaatatc gtgtccatca attggcccaa aatagcgaaa
     2881 acctaatttc tcaaagatta tccccggaac caggaacccc ttaacatgct cctctacttt
     2941 ggctaccata gtgcgaattt tatctcttct cttgaacttg ccaactaaat cccagacctc
     3001 tgcttttatc ttattgtact tttcatcggt caggatgttg gtcagatatt tggatagggc
     3061 gccaacgttt ttagagatgg acatggtatt gtcgttaagc accaccagca tgtttttact
     3121 taaagcgcca ctgttattca ttccttcaaa ggagagccca ccagtcaagg cgccatctcc
     3181 aaccacgact gctatgtgaa aattctgatt caaatagtca cgagcacaag ccattcctaa
     3241 agcagctgaa atggaggtgc tggcgtgtcc aacctcataa gcatcatgct cactctcttt
     3301 tttcttagga aatccgctta tccctttgta ttttcttaag gtactgaact tttcccttct
     3361 cccggtaata agcttatgcg tataacattg gtgaccgaca tcccagacaa ttttgtccct
     3421 gggtgaatcc agaacatagt gcaaagctat ggacaactca attgccccca agttggaagc
     3481 caaatgccca ccattctttg agacaacagt tattatctcc tcccttatct ctgccgccaa
     3541 cttgggcaag tcctcaagag gtatcttctt caaatcctgg ggtgaattta tgctgtctaa
     3601 taactttgcc atatggttcg acatactcac cattaacgga ttaatttgtt cggctgatta
     3661 gaaaatccgc caattgttta agtattcctg tttttttaaa actctttaaa tccttttttg
     3721 ctgactggac taatcttttt gcctcttgct ttgacctttc gactcctatg actttaggat
     3781 aagtagcttt ggctttgtct ttatcaaccc caactcgctt gcctaacttt ctctcttcac
     3841 cttccaaatc cagaatgtca tctgtgattt ggaaagccag tccgaattta ttgccataag
     3901 aggttaaggc tttaagttgt ttggaattgg ctttacctaa tatcgctccg accctgatgc
     3961 aagccctcaa aagtgcggcg gttttatgga tatgaatgaa attgacttct ttaagattaa
     4021 ctttctttcc ttctgcctct aaatcagccg cttgcccacc caccatgcca actgttccaa
     4081 tgctatttgc tacttcgacg acgattttag gattatcact tttcgctaaa agctcaaaag
     4141 ccagggcgtg caacgcatct ccagccaaaa ccgccatccc gtatccaaaa accctgtggc
     4201 aggttggctt tcctcttctc aagtcatcat catccataca aggcaggtcg tcgtgaatca
     4261 gagaataagt gtgtatcagt tcaagggcgc aggcaactgg cagaattgaa tttcctttgc
     4321 cccctaccaa ctcataagct gccatcgcca gaattggcct tatcctttta cctccggcaa
     4381 atacactata tcgcatagct tgatgcaact tacggggtgg tttgtggctg gatggaagat
     4441 atctatctaa agcgttatct attattcttt ttttatttcg caggtaatct tctggcttca
     4501 tctattcttc ttccttgaaa tcgaacggct ccaatttgaa ctttccatct gatagctcaa
     4561 ccaatttttc aaccttactt tgagcatcat tgagtttttg tgagcagaac ctgaaaagcc
     4621 ctatcccttc ctgaaagatc ttgagcgact ggtccaaggc taagtctccg ctttccagtt
     4681 gctccactat ctcttctaag cggctcaacc ctttttcaaa attcatcttc ttgggcaatt
     4741 ttcactcctc gtttatcttt ttgacttctg ccctgatatg accgagatga aatttgactt
     4801 cgacttcatc atctattttc aagcgggcag catctttgac cactgacagt ccaggaactt
     4861 ttcgggttat ggaatatccc ctttccagaa ttgccaaagg agatagggca ttcaggtgac
     4921 taaaaaaaga agataaccct tttttcttca attcaaaata atttttcatg accaacaaga
     4981 atgctcgcca cacctcgtcc gttctttgca ttttttgatt caacaggtca agtggcttcc
     5041 tgaacccata gctccgctgg atactttcaa gtctattttt attatatccc aaaatcgagt
     5101 tttgataatt gactatacga ccggctaaag agtttagttc cctttcaagc tcctttttgt
     5161 ctcgcaccac catctctgcg gcaacagatg gagtagctgc ccttaaatct gcaactaaat
     5221 cggaaatggt gaaatctatt tcgtgtccca ctgctgaaat tatgggtagt tttgacttga
     5281 agatggctcg ggctacgatt tcttcattga aagcccagag gtcttctaaa gaaccccctc
     5341 ccctgccaac tattatcaaa tcagcttttc catattgatt gaactgggca acggctcggg
     5401 caatttcatc ttcagcaccc tcactctgca ctctgaccgg atttaaaatc aactttatat
     5461 aaggggctcg acgctggatt atttttatta tatctcttat ggctgcccca gtaggggagg
     5521 taattatgcc tatggtttca ggaaatttcg gaattgcctt tttgtgctct tcgtcaaaca
     5581 gcccttcttt ttgaagtctc tctttcaatc tttggagttc taattccagc tttccaatgc
     5641 ccaatggtct taaaatctca acataaagct ggtattcgcc tcttttctca taaacggaga
     5701 tcttgcccaa cgccaaaacc ttcattccac tttgtggctc gaaatcaagc ccctcgcctt
     5761 ttcctcgcca taaaacgcat ctaatctgtg cttcctcatc ttttagagaa aaatatctat
     5821 gtccggaaga atgtagaacg aagttggaga tttctccctc aatccagata gttggcagtt
     5881 ccccttccag aagctctttt atctgtttgg ttatttcgga gacagtatag attttatcgg
     5941 gcatgttatt caactaattt cttgaaatca gggtcattcc aaacagtttt gaaatctgta
     6001 tcggttttgg atttttctct atatttatta tccagattaa tcgcatcttt caagtatttt
     6061 aacatctgat ttttatcttt caattcagca aatgcatagg ctaaattata atgggcctca
     6121 gcataatctg gcttaatctc tattgctttc ttgcatttct caatagcttc ttcatatttt
     6181 cctaacttaa ttaatgcaac accccagttg taataggctt cagttaaatc cggtttaagc
     6241 tctatagcct tcttgcattt ctcaataact tcttcatgct ttcctaactc acccaatgca
     6301 acgccccagt tgtaataggc aatagcatct tttggattga tttgaattgt tttcctaagt
     6361 atagatattt cttcttcata tctttgaagg cgatagtagg aaaatgccag ttgattgtaa
     6421 accagttttg catcttctcc aaatatctta tccgtgagct tctcatatct ttgtattgtt
     6481 ttttccgcat ccttttctga aattatggct ttggcttcct ctgaaaaatc cagtggggtt
     6541 tttgtctttt ttgcctcttc cgttatcttt tcagcgtccc taactatttc agctgccttg
     6601 gacctcattt gcttgatttc ttcgcacaat ccctccacct cttccttctt cttctgaacc
     6661 tcatctttta tttcctttgc cctctccaac tcaccttcca tttgttttat aagtgcccta
     6721 ccttgaaggt atccaaaaac acctaacgca attgcaaata aggagatagc ggctattact
     6781 ctaccagccc agcttacact actatttgcc ccctgtatcg ctttttcata agcatccttt
     6841 ttaaccttct ccaattcagc ctttaaggta tcaatctgcc cttgatactg cacagattga
     6901 gcaggagctt tgactgaatc aggagatttg gattgggcaa gagttgaaaa attacagagt
     6961 ataaagaaaa taacagaaaa cagaattagt gtcttgattt tcattcgatt ccccctttaa
     7021 ggttacgaaa aatataagct tgtaacaatt ctaagtcaaa tagtatcttt tgaggttaaa
     7081 agggacgaga ttgcttcgcc ccgattttat cgggactcgc aatgacatct tttcttcctt
     7141 ttgtcattgc gagaagtccc gccttcaggc gggacgacga agcaatccct tttgtcttcg
     7201 gcacaaatcc atttgtgtag cggaaagagt ggattcccgc tgtagtttat cccgcaaaat
     7261 gcggggcggg aatgacagta gaaatgtagg cgacattggc caatgtcgcc tacgactttt
     7321 cgttggttgg ggaattggaa ttttacgctt ttagcggaat cccggtgacc taaacctaaa
     7381 accctgcggg tcaccaggat tccgccagag gcggaaaaat ccccggcacg agtcttgacg
     7441 acaatttccg cataaacaaa aaaccggcga cggaagtcgc tgacctaccc aattccgctg
     7501 cataaaaatg tagggacagg tcgcgacctg tccctacaaa agaaatacag ataaatatta
     7561 caattatttg acttatttct ttaaattgct ttcaaggata actgcaacta aaatagaatg
     7621 tctcctaatg aaaaatatct tcttaactac tggcaacaag ctgctggcgc agggccgcct
     7681 ttaaagagat aattaatcag ataaactacg tctgagacgg ttactttccc atctccattg
     7741 gcatccccaa gacacttttc cggaaccgga acataaccac ctttaaacag gtagttaatc
     7801 aaataaacta cgtccgagac agttatcttg cagtcaccat tacagtcacc gcagctatag
     7861 tcaccacagt cataaataat tgacaccatc ccgtctctat aggtgttatc atctgtgtct
     7921 atctcccctg gaagtagagg agcataggca cttatagtgt aattacctaa aacaatgcct
     7981 gtagtattcc aattaaaact tatggtggtt tcttgactcg gagctaactc agtaacagtc
     8041 tttgtctgaa ttggaatatc atcgtaaaag gtggttacat caaaagtctc tctaacgctg
     8101 ccttgattct ggacagttac gtcaatagat acagaatcgc cttggactac tatttcttta
     8161 gaaggcacaa tatttatgac tgctatgtct atattaatgt ttaataaaac cgagacagtg
     8221 cggccacctt catttgcagt ggcaaggtca aggtcaccat cgccgtccaa atcagcagca
     8281 aagatagaac gggggttatt accaccaact ggatattctg tggaaggact aaaagtagcg
     8341 tttccattat ttaggaggac agagacattg tggcttccat aatttgcagt ggcaaggtca
     8401 aggtcaccat cgccatctaa atccgcagca aagattaagt ggggattatt accgccaact
     8461 gtgtattctg tagaagg
//
LOCUS       DASWLN010000162        27651 bp    DNA     linear   ENV 20-FEB-2024
DEFINITION  MAG TPA_asm: Candidatus Zixiibacteriota bacterium isolate
            SMAG_U2949 k141_1216084, whole genome shotgun sequence.
ACCESSION   DASWLN010000162 DASWLN010000000
VERSION     DASWLN010000162.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37960594
            Sequence Read Archive: SRR10376091
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Candidatus Zixiibacteriota bacterium (soil metagenome)
  ORGANISM  Candidatus Zixiibacteriota bacterium
            Bacteria.
REFERENCE   1  (bases 1 to 27651)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 27651)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (26-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/12/2024 08:28:26
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 1,440
            CDSs (total)                      :: 1,416
            Genes (coding)                    :: 1,412
            CDSs (with protein)               :: 1,412
            Genes (RNA)                       :: 24
            rRNAs                             :: 1 (16S)
            partial rRNAs                     :: 1 (16S)
            tRNAs                             :: 21
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 4
            CDSs (without protein)            :: 4
            Pseudo Genes (ambiguous residues) :: 0 of 4
            Pseudo Genes (frameshifted)       :: 0 of 4
            Pseudo Genes (incomplete)         :: 3 of 4
            Pseudo Genes (internal stop)      :: 1 of 4
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..27651
                     /organism="Candidatus Zixiibacteriota bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_1216084"
                     /isolate="SMAG_U2949"
                     /isolation_source="soil"
                     /db_xref="taxon:2053527"
                     /environmental_sample
                     /country="USA: Oregon"
                     /lat_lon="42.99 N 123.63 W"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            <1..612
                     /locus_tag="VGB16_05330"
     CDS             <1..612
                     /locus_tag="VGB16_05330"
                     /EC_number="2.4.2.1"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_014856772.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004731 - purine-nucleoside phosphorylase
                     activity [Evidence IEA];
                     GO_process: GO:0009116 - nucleoside metabolic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="purine-nucleoside phosphorylase"
                     /protein_id="HEX9917141.1"
                     /translation="EKKVVAMQGRFHYYEGYNMQQITFPVRVMKALGANVLVVSNACG
                     GLNPLFKAGDLFLITDHTNLQGANPLIGVNDETLGPRFPDMYACYDREFIKLAEETAL
                     ELKIKLQKGVYVGVSGPNLETAAEYRFLRMIGADVVGMSTVPEVIVARHQSMRVLGLS
                     IITDMGLPDALEPTNLDKIIATANRSEPILRNLMSRVIEKMKL"
     gene            632..1549
                     /locus_tag="VGB16_05335"
     CDS             632..1549
                     /locus_tag="VGB16_05335"
                     /inference="COORDINATES: protein motif:HMM:TIGR01297.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008324 - monoatomic cation transmembrane
                     transporter activity [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="cation diffusion facilitator family transporter"
                     /protein_id="HEX9917142.1"
                     /translation="MSEHTHSHNQQLASNRKALIVALSITLIMTVVEVVGGILSNSLA
                     LLGDAGHMLTDSLALGLSLLAVQMCCKPPDSKRTYGYYRTEILVALLNGLTLALISFY
                     IFYQAYNRFTNPPQVRAPLMLVVATIGLIANLLGILVLRRGSQENLNVKGAFLHILGD
                     TLSSVGVIVGGIIIYFTNFYLADPIIAVLIGLIILKGAMGLLFQSTDILLEAVPKHLN
                     LEFIKSELEKMEGIKGVHDIHIWTITSGIYALSGHLFIDDQMISKTKQILENVNQLLK
                     EKFRISHTTLQIECTECNQLSVCSLNERI"
     gene            1572..4736
                     /gene="ileS"
                     /locus_tag="VGB16_05340"
     CDS             1572..4736
                     /gene="ileS"
                     /locus_tag="VGB16_05340"
                     /EC_number="6.1.1.5"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_013777794.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0005737 - cytoplasm [Evidence IEA];
                     GO_function: GO:0004822 - isoleucine-tRNA ligase activity
                     [Evidence IEA];
                     GO_process: GO:0006428 - isoleucyl-tRNA aminoacylation
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="isoleucine--tRNA ligase"
                     /protein_id="HEX9917143.1"
                     /translation="MLFKETKEDFNYPQREEEILSFWKKNGIFQKSIQIRKDAPLYVF
                     YEGPPTANGLPGIHHVIARIIKDLVCRYKTMQGFRVDRKAGWDTHGLPVEIEVEKELK
                     LDSKAKIEEYGIDKFNAKCRESVFRYKKDWDKLTERIAYWLDLDDAYMTCSNEYIESV
                     WYILSQLFEKGLIYQGHKIIPYCPRCETPLSSHEVAQGYDEISDPSVFVKMELEDKPN
                     TYFLVWTTTPWTLISNIALAVNSQLDYVKVEHKGENLIMARNLLHQVLGEDYRILEEM
                     KGKDLEKIRYKPLYNFIPVKKDEGYFVVLGDFVSLEDGTGIVHIAPAFGADDYEVGKK
                     YSLKLLQPVDTSGKFTSEIKNWANMFIKDADPKIIQDLKNRRLLYKEEKYVHNYPFCW
                     RCGSPLIYYARKSWYIQTTAFKDKMIANNQKINWYPPDIGQGRFGEWLENNVDWALSR
                     ERFWGTPLNLWICEKCKEVKSISSLEELRRLGQNVPEELDLHKPMIDEIALVCEKCQG
                     QMKRTPEVIDCWFDSGSMPYAQWHYPFENQENFKAHFPADFISEAVDQTRGWFYSLLA
                     ISTSLFDQLAYKNVIVIEFIQDKFGVKMSKSKGNAIDPWEVLNIQGADTLRWYLLYSS
                     HPWLPTKIDKEAITEVQRKFFSTLRNSYSFFALYANIDKFDPRKHRGELKFVSEIDYW
                     LLSRLNTLVKNTTNSLDNYDITGAARLIQDFVIDDLSNWYVRRNRRRFWKSGEDEDKL
                     SAYSTLYNTLVTVSQLIAPYIPFLAEELYQELVAKIDKDAPSSVHLTFFPKPKDNFIN
                     IQLEEKMGERKRIVSLGRAARNKAKLKIRQPLADMWLPSQLETIFSETSSPGALGTLM
                     EELNVKNIHFTDKRIPTELVVRPNFSVLGPKFGEKAKTVADRLKNLTITEIEKLKKEG
                     SLELKLEEEIIKVELDQVTIEEKDPEGFATESDDSYQVALSTKLDRELENEGLARELV
                     NKIQNMRKSAGFEVMDQIKIEIKATSRVEEAVTAFEDYIKKETLAKSISFSLDSTDTF
                     KEWNINGEKTQISVNRIKTIGGNK"
     gene            4733..5119
                     /locus_tag="VGB16_05345"
     CDS             4733..5119
                     /locus_tag="VGB16_05345"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_006929685.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008270 - zinc ion binding [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="TraR/DksA C4-type zinc finger protein"
                     /protein_id="HEX9917144.1"
                     /translation="MKKKELEKYEKLLLAKRAELLKELGLLKKAAMDTTIKDSTGDLS
                     SYSYHMADQGTDAMEREKAFLFASKSGRLLYHIDEALRRIKDKTYSKCQRCGKQISQA
                     RLEAVPHARFCIACKEKEEREKTEEI"
     gene            5129..5635
                     /gene="lspA"
                     /locus_tag="VGB16_05350"
     CDS             5129..5635
                     /gene="lspA"
                     /locus_tag="VGB16_05350"
                     /EC_number="3.4.23.36"
                     /inference="COORDINATES: protein motif:HMM:TIGR00077.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0005886 - plasma membrane [Evidence IEA];
                     GO_function: GO:0004190 - aspartic-type endopeptidase
                     activity [Evidence IEA];
                     GO_process: GO:0006508 - proteolysis [Evidence IEA];
                     GO_process: GO:0009306 - protein secretion [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="signal peptidase II"
                     /protein_id="HEX9917145.1"
                     /translation="MIFLLLSLLVALLDQLSKGYITSHFHLGQSISLLGDFLKITYIQ
                     NPGGVFGTRIGSGNFYTFISLVAILIVLLVYFRMRNQEATFKIALGLILGGAIGNLID
                     RFRFGEVVDFLDVDFFNINLPPFKFLFLKFNGFYLDRWPVFNLADSAVTVGLVILIYH
                     FSFQGAKN"
     gene            5642..6628
                     /locus_tag="VGB16_05355"
     CDS             5642..6628
                     /locus_tag="VGB16_05355"
                     /EC_number="5.4.99.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_009610096.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003723 - RNA binding [Evidence IEA];
                     GO_function: GO:0009982 - pseudouridine synthase activity
                     [Evidence IEA];
                     GO_process: GO:0001522 - pseudouridine synthesis [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="RluA family pseudouridine synthase"
                     /protein_id="HEX9917146.1"
                     /translation="MNVKTLEIKSSLGDEGKRLDVFLVEKKLGLSRSRIQDLISQQEV
                     LVNNKSVKPHYKIKGNETIFISLPLPVESEIKPENIPLDILYEDKDLLVVNKPAGMVT
                     HPAAGNYSGTLVNALLFHCKDLSGINGVLRPGIVHRLDKDTSGLLVVAKNDFTHNLLA
                     EQLKERKLFRQYWALTCGNMPTWEGKIEVPIGRSTKDRKKIVANPPRSKESITLYQVL
                     ERFEISDLLCLKLLTGRTHQIRVHLSYLGKPVLGDETYGGRKRWTNQLGESQKDFAYR
                     ILELLPRQALHAKKIGFVHPRTKEYIEIESDLPLDMSRALKLLRGEKNALSH"
     gene            6612..7397
                     /locus_tag="VGB16_05360"
     CDS             6612..7397
                     /locus_tag="VGB16_05360"
                     /EC_number="2.7.1.33"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003515466.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="type III pantothenate kinase"
                     /protein_id="HEX9917147.1"
                     /translation="MLLAIDIGNTHTVIGIFVGNRLKNDFRFYSRHTLTADECIMLVS
                     QLFSQEKIQKENIQGVIICSVVPNLTPIFQEASRKYLKVKPIIVGPDLDLNIKIKYDL
                     PQQVGADRIANAVAAYHLYKGPTIVVDLGTATTFDAISGDGEYLGGAIAPGIETSSAN
                     LFHRAAQLYKVGLDKPQKAIGTNTTESLKSGIVFGAIGQIDEITRRIKKELRGKVFVV
                     ATGGLAKLVAGESKTVQKINPTLTLQGLRIIYNMVTAKKKKRG"
     gene            8351..8638
                     /gene="groES"
                     /locus_tag="VGB16_05365"
     CDS             8351..8638
                     /gene="groES"
                     /locus_tag="VGB16_05365"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002770236.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_function: GO:0016887 - ATP hydrolysis activity
                     [Evidence IEA];
                     GO_process: GO:0006457 - protein folding [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="co-chaperone GroES"
                     /protein_id="HEX9917148.1"
                     /translation="MKVKPLADRVLVKPLETEETKKGGIIIPDTAKEKPQEGEIMEVG
                     PGRITEDGKKIPMEVKKGDKILYGKYSGTEVTIDNVDYLIMRESDILAIVG"
     gene            8683..10314
                     /gene="groL"
                     /locus_tag="VGB16_05370"
     CDS             8683..10314
                     /gene="groL"
                     /locus_tag="VGB16_05370"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_020258109.1"
                     /note="60 kDa chaperone family; promotes refolding of
                     misfolded polypeptides especially under stressful
                     conditions; forms two stacked rings of heptamers to form a
                     barrel-shaped 14mer; ends can be capped by GroES;
                     misfolded proteins enter the barrel where they are
                     refolded when GroES binds; Derived by automated
                     computational analysis using gene prediction method:
                     Protein Homology.
                     GO_function: GO:0005515 - protein binding [Evidence IEA];
                     GO_function: GO:0016887 - ATP hydrolysis activity
                     [Evidence IEA];
                     GO_process: GO:0006457 - protein folding [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="chaperonin GroEL"
                     /protein_id="HEX9917149.1"
                     /translation="MAKIIEFSAEAREKLKKGVDKLANAVKVTLGPKGRNVVLDRKFG
                     SPTVTKDGVTVAKEIELEDNFENMGAQMVKEVASKTSEVAGDGTTTATVLAQAIYREG
                     LRNVTAGANPMALKRGIEKAVEKVVEELKKISKPLPGKTEIAQVGTISANNDKEIGNL
                     IADAMDKVGKDGVITVEEAKTTKTELEVVEGMQFDRGYLSPYFITNPDSMEAMLEDPL
                     ILIHDKKISVMKDLLPILEKTVQMGRPMLIISEDVEGEALATLVVNKLRGTLKVCAVK
                     APGFGDRRKAMLEDIAVLTGGKVISEELGLKLEKTLENDLGKAKRVTIDKDNTTIVEG
                     GGKTEDIKGRVNQIKREIEETTSDYDKEKLQERLAKLAGGVAVINVGATTETEMKEKK
                     ARVEDALHATRAAVEEGIIPGGGVAYLRAIPALEKLKLEGDEAIGASIVKKALEEPIR
                     LIVENAGVEGSIVVNRVKSEKGSFGFNAETEKYEDLITAGVIDPTKVARIALENAASI
                     ASLLITTEAIIADKPEEKKSPMMPPGGGYGGGDMY"
     gene            complement(10756..11997)
                     /locus_tag="VGB16_05375"
     CDS             complement(10756..11997)
                     /locus_tag="VGB16_05375"
                     /inference="COORDINATES: protein motif:HMM:NF012958.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="alpha/beta hydrolase-fold protein"
                     /protein_id="HEX9917150.1"
                     /translation="MKNWHKLSALIFLLVTLFAYIGCSDRKNPADPVYPKGTVYFQAV
                     DSSTLGRNTALSDNLITNPYGGNAFVYTPPGYDGADASTEYPILYFLHGFMDTTLGYI
                     PTTPDFPYPYGLAQTADRLIATGQIQPMIIAMMNCNSYLGGSIPAGSFYVNSPSPTSD
                     PNQSLNGKFENFYVDDFMYRVEKSFYVHQDKSYRAIAGHSMGGYGAFRIAMDYPDIFS
                     IVGSMSGPLSFGDWDLNSAIDHIFSVNGVFDSTAYRDSLNEAYQGPDEITRMFFAMGA
                     AFSPHSTTNPDSTSYFKVTPQGTTTTYGVDLPFDSTQNIIASVWNRWIDSNDVQTIYG
                     RGTAVLDSMAIYIDCGDADEFEFNQQSSDFYQGLTSGQKAHSFFEEYSGGGYLSADNS
                     TYLYYRIEKLLKFVSENFPQP"
     gene            complement(12022..13404)
                     /locus_tag="VGB16_05380"
     CDS             complement(12022..13404)
                     /locus_tag="VGB16_05380"
                     /inference="COORDINATES: protein motif:HMM:NF015316.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="outer membrane protein transport protein"
                     /protein_id="HEX9917151.1"
                     /translation="MASKHLNSVSFVVLALILIGSTAWGAGFAVNGIGTKATSLGGGF
                     RGLADDWSACFWNPAGLGFLESSELNTSLSASLFLPKYNPDVLYGDLYEVGFKNGAYR
                     YPENRFYFLPNFSGFYKLPKQNIVVGMGFYTSYELESHYWSVFEPLPGYDNLVSYPKD
                     DYGPNIMIVDFHPTLAKAFSEKLSLGLGLSFNWADFKLTKVTLAQTGYDAPLQNIPID
                     SKLKMDGWGVGFNLGMLYKISPKMQMGVSITSPVNIKVEGDMNYTAYLPNHIEVDTSF
                     TGGTLASIPKAKATLNLPASAGVGFAYFSSDKLTFTFDGSWTGWSRWDELEVNLTGKD
                     PFGETATSPTLAYNWNDVFKIALGCEYMYKDKLAIRGGYSFESSLIPNSTLDIIVPDV
                     GPKHGFSLGLGYEFENNIELGLGQQLVLSPSKTIDKIEDANNDGVYDNLPGEYGNMSY
                     STHLSLTYRF"
     gene            13694..14488
                     /gene="mazG"
                     /locus_tag="VGB16_05385"
     CDS             13694..14488
                     /gene="mazG"
                     /locus_tag="VGB16_05385"
                     /EC_number="3.6.1.9"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011916752.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="nucleoside triphosphate pyrophosphohydrolase"
                     /protein_id="HEX9917152.1"
                     /translation="MEKRRGHQEYFKELVSLMAMLRGKNGCPWDRSQTHNTLLPYLIE
                     ETYEVVEAVQSKDYERLKEELGDLLLQIVFHSQIAKEKNRFDVYEVARQINLKLRQRH
                     PHIFENKKKISKQEVIQNWEHIKLSQKKDKGVLSGLPRQLPGLLRAYRLQEKVGRLNF
                     DWKNAADVLPKLKEELGEFEKAFKTKNKRKMEEEMGDILFSWVNLCRHLNINPELALG
                     KTVDKFIRRFNYIEKALAKKRIGFDQADLPFLDSLWEKAKKRKNRK"
     gene            complement(14784..16268)
                     /locus_tag="VGB16_05390"
     CDS             complement(14784..16268)
                     /locus_tag="VGB16_05390"
                     /inference="COORDINATES: protein motif:HMM:NF024895.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="OmpA family protein"
                     /protein_id="HEX9917153.1"
                     /translation="MKRLTLLLVALLVVWMAVSANAIDLTGKFGLSVHPAMFKPILAD
                     HSDAWAVGPEGSVILKYGFTPNVAVGVMGTYSFTKEADLTGKTDGAGFTFSTDPNGYK
                     FNAMMVEAVAFYHFMPEEKFNPYVFGGAGIAFWNWKDKDGNAVLWPDTSELDSFEVKD
                     QELTAVFGVGLDYYVTENIALNIGGKAHYLTHVLTSLKNEKDPEEVGLDLPKLIPEAY
                     VGATFYFGKPKDSDKDAVPDKVDQCPDTPLGAMVDENGCPLDADNDGVYDGLDKCPNT
                     PKGAKIDVNGCPIDSDGDGVFDGIDQCASTPKGVKVDAKGCPMDSDGDGVADYLDKCP
                     NTPKGAKIDVNGCPIDSDADGAFDGIDQCPNTPAGVAVDEKGCPKVAAIEKITLHIKY
                     ASGSANLDAPAMTALDDLAQRLSFYKDVKIEVLGYTDATGTLAANMRISKLRADGVKN
                     YLVKKEVDAARIEAKGMGPENPIASNDTPEGKQENRRIEIVPVK"
     gene            complement(16448..17695)
                     /locus_tag="VGB16_05395"
     CDS             complement(16448..17695)
                     /locus_tag="VGB16_05395"
                     /inference="COORDINATES: protein motif:HMM:TIGR02669.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="SpoIID/LytB domain-containing protein"
                     /protein_id="HEX9917154.1"
                     /translation="MRRHINITLSFLLALFILNCAQLPAVRQGVFYSQVKQPMVAVKL
                     LEGREISVSPANNFAIRCFRNGIQTANYFSTADIEVRVDSSFIIWGQKNQPPMESNLS
                     KVIFEPKSRENWLSLNGKRYRGALEIVLDQNKNLLVVLNIVHLEDYLKGVVPAEMGKR
                     KKGEIEALKAQAIAARTYALSKMGQYDGVYDLKATIYDQVYADMDGEESLVNQAVDQT
                     EGKILTYQDKLIQAYYYANCGGRTESLEEVWSGMGEPYLLSVEDGDFCTWAGNYRWEE
                     EWSRKELEERLFEFLSEKEGITEEDFGRLLDLKILDRAPSGRVKVLLVSTDKEDFIIE
                     KDKIRWALKRTKDVILPSTLFDLEITRGIDGSLIKAKAVGRGSGHGIGMCQTGAIGMA
                     NRGLSYSEILAHYYPKTKVLKYY"
     gene            complement(17692..18867)
                     /locus_tag="VGB16_05400"
     CDS             complement(17692..18867)
                     /locus_tag="VGB16_05400"
                     /EC_number="2.7.1.170"
                     /inference="COORDINATES: protein motif:HMM:NF007139.0"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="anhydro-N-acetylmuramic acid kinase"
                     /protein_id="HEX9917155.1"
                     /translation="MDKLFSLLKKKELLVTGLISGTSADGIDVCLVRIKEVKDDLKIK
                     LLGFKTYEYADSIREKIFELYKDKNHNLDDLVRLNMLLGKSFARAVIDLCKKIGIAKN
                     RLDLIGSHGQTIRHLPKVANFCGKKIRGTLQIGEPAVIAALTGSVTIGDFRQADMAVG
                     GEGAPLCPLAHFHLFKHRDFARAVLNIGGIANITLIPKKGKMKDIFAFDTGPGNVLMD
                     TLVNKLYSKEYDKNGEIALKGKVNPDLLRKLKRNSYFKKKPPKSTGREDFEENLRYIL
                     GTKRIPKQDVIATVTELTCWSIFDAYKRWVRPKTKIDQLVVCGGGSHNRYITKRLSML
                     FHPIRVLSSEKLGYNPDQIEAICFAILAYLTMKNKKGNLREVTGAKKEVRLGKICLP"
     gene            complement(18860..19849)
                     /locus_tag="VGB16_05405"
     CDS             complement(18860..19849)
                     /locus_tag="VGB16_05405"
                     /inference="COORDINATES: protein motif:HMM:NF013126.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004553 - hydrolase activity, hydrolyzing
                     O-glycosyl compounds [Evidence IEA];
                     GO_process: GO:0005975 - carbohydrate metabolic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="glycoside hydrolase family 3 N-terminal
                     domain-containing protein"
                     /protein_id="HEX9917156.1"
                     /translation="MTPFTDNWAQVFVLGYKGLYPSDEFVDLVKQYKIGGVIIFSENI
                     EDSRQLREVILDLQSISPIPLFVMIDQEGGKTNRITKDFPTFPSNSFFGQRKDEKGVE
                     QAYSITARELRNLGINANLAPVVDVLTNPQNELLKERSFGNDAKLVARFTKVAVQAIR
                     SQGVFSCAKHFPGLGNAALDPHKELSIDQNEKTKFEQINFIPFKSAIKTGVEMIMTTH
                     ILCEKLEPTFPATLSETICRDILKKELGFDGLVITDDMGMGGITRNWDINFACKKAFL
                     AGHDLILVAKRWEKQKEILESFEKGIKDGETKKGKVKSSVDRILKLKKRLKNG"
     gene            complement(19861..21429)
                     /locus_tag="VGB16_05410"
     CDS             complement(19861..21429)
                     /locus_tag="VGB16_05410"
                     /EC_number="2.7.7.65"
                     /inference="COORDINATES: protein motif:HMM:TIGR00254.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0009975 - cyclase activity [Evidence IEA];
                     GO_process: GO:0009966 - regulation of signal transduction
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="diguanylate cyclase"
                     /protein_id="HEX9917157.1"
                     /translation="MISQDKGVLLERLSSFNNMFALVRSVLFLGFSIWLSFSYRGSEY
                     NVGMILAFVFLLHLLVFSIISRKTKKHGKLLLFSSIADILFITFLVYFTGGVKSNFYL
                     LYYLSISFTAYYLGLYEGLALSLGSTILYLIIYQEKGSGNIFIGDLAIRILFLWMFAF
                     AVGAISKYLKLSRAKLFRALDTLNERTSELEKSQVQIETIYETSGTLGAIHDLDQVID
                     ETLNIVKEVLGYQMCSVLLLDSRRNVLLLKAKVELGNKIKYSPPEEVPLTGIAGTVAR
                     LGKPERVFDIRTDPRYIYGLYNARSELAVPMISQGKVVGVLNAESKRVGAFLEQDEKI
                     FSILASSAGMAIENAMLHQEMEELTIIDELTGIYNYRYFVQKLEDELRRARRYEQILC
                     LIMIDIDCFKRYNDSYGHLFGNSILRGLVQVVKGCIRDVDIFARYGGEEFVVILPQTD
                     KYDAANIGERIRSQVESTQFEGEEKGLKVNLTVSVGIASYPENGTSSEILIQKVDLAM
                     YIAKGRGKNLVCTI"
     gene            complement(22349..22864)
                     /locus_tag="VGB16_05415"
     CDS             complement(22349..22864)
                     /locus_tag="VGB16_05415"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEX9917158.1"
                     /translation="MNESAQEPFSCKTYPWHVIILRWMVLSIIFALGIYIIYQLNQIL
                     AWLYILYSIFGLTLVLPLSRCVYCYYHNRWCNTGWGKLAAYLFNKGDEEKYVSKYPYA
                     ILLYPLWFFPLLLGIILLIRYRNLASLVLLLVYVSLLVIEKLILRFAACSKCQQKGFC
                     PALPFRGNKVF"
     gene            22954..24087
                     /locus_tag="VGB16_05420"
     CDS             22954..24087
                     /locus_tag="VGB16_05420"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="polymer-forming cytoskeletal protein"
                     /protein_id="HEX9917159.1"
                     /translation="MSKKIFSIVIFALFLLTACFKFSLATQFRAGDNLIFAPDKKIDD
                     DLMAAGGKIKFNGVLSGDLLVAASSVAQNGTIQGSFMAAAQQIDISGPIDRSSRTFSQ
                     YVNISSSVNQNVMAFCQRCDLKSTGYVGKDFHAFCSELNVDGKIKGNLKGYNQTVTIS
                     GTIEGDVSLVAERIVLMPTAKINGNLNYKSPNEADIQKGASVLGKTQWKKTEPKKKGT
                     APGRFILNRFLSFLGVLIPGLILIPIFKEQTQRIKTNLKNSPFKSLAIGFIFLICMLV
                     IFIMFFMLSFVIIGIPLAVLSFMVILSMLFLCKIAVGVALGDWLLSFLSSNGNVNTIL
                     ALLVGLIILTLLTSIPYFIGTLFYLIILFSGTGAIIYVKRKAS"
     gene            complement(24519..27512)
                     /gene="secA"
                     /locus_tag="VGB16_05425"
     CDS             complement(24519..27512)
                     /gene="secA"
                     /locus_tag="VGB16_05425"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_006926757.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_process: GO:0006605 - protein targeting [Evidence IEA];
                     GO_process: GO:0006886 - intracellular protein transport
                     [Evidence IEA];
                     GO_process: GO:0017038 - protein import [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="preprotein translocase subunit SecA"
                     /protein_id="HEX9917160.1"
                     /translation="MFGNILTKIFGSKHQRDIKKIMPIVALMNQHYEEYQKLSDDQLK
                     AKTIEFKERLAKEETLDDILPEAFAVVKDVCRRLLGKRWEVCGIEIEWDMVPYDVQLV
                     GAVVLHKGKIAEMATGEGKTLVATMPIYLNALTGKGVHLVTVNDYLAKRDSQWMGEIY
                     KFLGLTIGCIQNDMDSQKRKEQYDCDITYGTNNEFGFDYLRDNMAIRVEDRVQRGFNY
                     AITDEVDNVLIDEARTPLIISGPVSFSTTQRYSEMKPLVDKLVKKQAVLVNQFTSEAE
                     KSLGEGKTYEAGVKLLAVQRGSPKNKKFMKLLKETGMKKLITTVEMDYLRDKKINEID
                     EMLYYAIDERENSINMTEMGRSLLDPSDQALFVIPDIAEGLHKIDSDATLSEKVKQEK
                     KEELYKLYAERSDKNHSITQLLKAYALFEKDVEYVVQDGKVMIVDEFTGRLLPGRRYS
                     DGLHQAIEAKEEVRVEGETQTFATVTLQNFFRLYGKLAGMTGTAETEAAELWEIYKLD
                     VVVIPTNESVRRIDYDDVIYRTRREKYNAIIDEIEACHKQGMPVLVGTISVEVSETLS
                     RMLKRKGIPHSVLNAKYHQKEAEIIVQAGRSGAVTIATNMAGRGTDIKLGESVVKSKK
                     CRLITPEREDGCPHLEELDCYENVPCGLRIIGTERHEARRIDRQLRGRSGRQGDAGSS
                     RFFLSLEDDLMRLFGSDRIATVMDRLGVGEGEVIQHPMVTKAIQRAQRRVEVQNFAIR
                     KHLLEYDDVMNQQREVIYDRRLAALEGQDFKEEIVDLIGQVVDSKVEQYAPLNTYPEN
                     WNLTGLRSELLKTFLVDLRFEEKDIPNLTQDALKEKIRQIAMEVYTNKEKTIGSQLMR
                     ELERYAMLRVIDDKWKEHLYEMDQLKTGIGLRAYGQKDPLIEYKKEAFAMFSQMIETI
                     DQEVVELIFKLQIADQSVKSRKEEREEIKTIHQSVSGMDYALAQPEGEGATKVKTVRR
                     EKAKVGRNDPCPCGSGKKYKKCCGA"
BASE COUNT         7990 a         6098 c         5763 g         7800 t
ORIGIN      
        1 gaaaagaagg tcgtagctat gcagggtcgc tttcattact acgaaggata taatatgcag
       61 caaatcactt ttccggtcag ggtgatgaag gcattgggcg ccaacgtgct cgtggtttcc
      121 aatgcttgtg gcgggctcaa tccactcttt aaggcagggg atttgtttct cataaccgac
      181 catacgaacc ttcaaggagc caaccccttg ataggtgtaa atgacgaaac cttggggccc
      241 agattcccgg atatgtatgc ctgctatgac cgtgaattta tcaagttggc tgaagagact
      301 gctctggaat tgaagataaa gctgcaaaaa ggggtttatg tgggtgtgag cggtcccaat
      361 ctggagactg cagcggaata taggtttttg cgtatgatcg gtgcggatgt ggtgggaatg
      421 tcaactgtgc ccgaagtaat tgtagcaaga catcaatcta tgcgggtcct ggggttgtcc
      481 atcatcactg atatgggctt gccagacgcc ttagaaccaa ccaatctgga taaaatcatt
      541 gccacggcca ataggtctga gccgatctta aggaatctga tgagtcgggt catagaaaag
      601 atgaaactgt aaaaacttta ggaatatata aatgtccgaa catactcata gccataatca
      661 gcaactggca tccaaccgaa aagcgcttat agttgccctt tcaatcaccc tgattatgac
      721 agtggttgag gtggtgggag ggatattaag caacagtctt gccctcttag gtgatgccgg
      781 acatatgttg accgatagcc tggcgttggg tttgagtctt ctggccgttc aaatgtgctg
      841 taagccgcct gatagcaaaa gaacttatgg atactaccgc acggagatac tggttgccct
      901 gttaaatggt ttaaccttgg ctttaatctc tttttatatt ttctatcagg cctataatcg
      961 ctttaccaat ccacctcaag taagagctcc gcttatgtta gtggtggcaa ctatcggtct
     1021 tattgctaac cttctgggaa ttttagtgct aagaagagga agccaggaga acctaaatgt
     1081 gaaaggagca tttctgcaca ttttaggcga tacgctttct tcggttggag taattgttgg
     1141 cggaattatt atctatttca ccaatttcta tttagctgac ccaattatcg cggtgctgat
     1201 cggcctgatt atattgaaag gtgctatggg attacttttc caatcgacag atatcctttt
     1261 ggaggcagtt cccaagcatc tgaatttaga gtttataaaa tccgaacttg aaaagatgga
     1321 agggataaaa ggagtgcacg acattcacat ttggaccatc acctcaggaa tctatgctct
     1381 ttccgggcat ctgtttatag atgatcagat gatcagcaaa accaaacaga tccttgaaaa
     1441 tgtaaatcag cttttaaagg aaaagttcag gatatcgcat accactttac agatagaatg
     1501 tacggagtgc aatcaattgt ccgtatgtag tctgaatgaa cgaatttaga tgatgtccta
     1561 aggagaacaa attgctgttc aaagagacta aagaggattt caattatcct caaagggaag
     1621 aggagatact ttccttctgg aagaaaaatg gcatctttca aaagagcatc cagatacgga
     1681 aagatgctcc gttatacgta ttttacgaag gtcctcccac agccaatggc ttgcctggaa
     1741 ttcatcacgt catcgcgcga attatcaagg atttggtctg ccgctataaa actatgcagg
     1801 gctttcgagt ggatagaaaa gcgggctggg acacacacgg cttgccagtc gaaatagagg
     1861 tggagaaaga gctgaagttg gatagtaaag ccaaaatcga ggagtatggt attgacaagt
     1921 tcaacgccaa atgccgagaa agcgttttca gatacaaaaa agattgggac aagttaacag
     1981 agagaattgc ctactggctg gatttggacg acgcctatat gacttgctcc aatgaatata
     2041 tcgaatcggt gtggtatata ctttctcagc tctttgaaaa aggattaatc tatcaaggac
     2101 acaaaatcat cccttactgc cctcgctgcg aaactccgct ttccagccac gaagtggcac
     2161 aggggtatga tgagatctcc gatccttctg tttttgtgaa gatggaactg gaggataagc
     2221 ccaataccta ttttctggtg tggacaacta ctccctggac cttaatctcc aatatcgcct
     2281 tagcagttaa ttcccaatta gactacgtca aggtggaaca caagggtgaa aatctcataa
     2341 tggcgcgaaa cttactccat caggtcttgg gtgaggatta caggatattg gaggagatga
     2401 aggggaagga cttagaaaaa atcagataca aacctttata taattttatc ccggtaaaaa
     2461 aagatgaggg gtattttgtg gttttgggag attttgttag tttggaggat gggactggaa
     2521 tcgtgcatat cgcccctgct tttggagcgg acgattacga agtcgggaaa aaatactctt
     2581 taaaacttct gcaaccggtt gataccagtg ggaagttcac ctctgaaatc aagaattggg
     2641 caaatatgtt cattaaagac gcagacccga agatcatcca ggacctaaaa aacaggagac
     2701 tgctttacaa ggaagagaaa tatgtgcaca actatccttt ttgttggaga tgtggttcac
     2761 ctttgattta ctacgcgcga aaatcgtggt acatccaaac caccgcattc aaggacaaaa
     2821 tgatcgccaa caaccaaaag ataaactggt atcctccgga tataggccag ggtcgcttcg
     2881 gggagtggct ggaaaacaat gtggactggg cactttcccg tgaaaggttc tgggggactc
     2941 ctcttaactt gtggatatgt gaaaaatgca aagaagtaaa atcaatctcc agcctagagg
     3001 agttaagaag attagggcaa aatgttccag aagagctgga cctgcataag ccaatgatag
     3061 atgaaatagc tttggtatgt gagaaatgtc agggtcaaat gaaaaggact ccagaggtga
     3121 ttgactgttg gtttgactcg ggttcaatgc cttatgccca atggcattac ccctttgaaa
     3181 accaggagaa tttcaaagcg catttcccgg cggatttcat aagtgaggcg gttgaccaga
     3241 ctcgtggttg gttttatagt cttttagcta tctccacatc tcttttcgac cagctggctt
     3301 acaaaaacgt catcgtgatt gaattcatac aggataagtt tggagtgaag atgagcaaat
     3361 ccaaaggaaa tgctattgac ccctgggagg ttctaaatat tcagggagcg gataccctaa
     3421 gatggtatct gctctacagt agccaccctt ggttgcccac caaaatagat aaagaggcta
     3481 tcactgaagt ccagagaaaa ttcttcagca ccttgaggaa cagttattcc ttttttgcgc
     3541 tctatgccaa tatcgataaa ttcgatccca gaaaacaccg gggggagcta aagtttgttt
     3601 cggaaataga ctattggttg ctctcgcggt taaatacctt agtgaagaat acaaccaatt
     3661 ccttggataa ttatgacatc accggcgctg ccaggcttat tcaagatttt gtgatagatg
     3721 atttgagcaa ctggtatgtc aggagaaatc gcagacgttt ttggaaatca ggggaggatg
     3781 aggataagct ctcagcatat agtacacttt ataatacctt ggtgactgta tctcaactta
     3841 tagctcctta tattccattt ctggcagagg agttgtatca agaattagtg gctaaaatag
     3901 acaaagatgc tccatccagc gtgcatttaa ctttctttcc caagccaaaa gataatttta
     3961 ttaatataca gttagaggaa aaaatggggg agcgtaaaag aatcgtttcc ttgggtcgtg
     4021 ccgcccgtaa caaagccaag ctaaagataa gacaaccatt ggctgatatg tggttacctt
     4081 ctcagctaga aacgatattc agcgaaactt catcacccgg tgccctcggt acgttaatgg
     4141 aagaattgaa tgtgaaaaat atacatttta ctgataagag aattccgact gaactcgtag
     4201 ttagaccgaa tttctcggtt ttgggaccaa agtttggaga aaaagcaaaa actgtggcgg
     4261 ataggttaaa gaacttgaca attactgaaa ttgagaagtt aaaaaaagaa ggcagtttag
     4321 agcttaaact tgaagaagaa attatcaagg ttgagctaga ccaagtgacc atagaagaaa
     4381 aagacccgga aggatttgcc actgagtcgg atgatagcta tcaagtggct ttatccacta
     4441 agttagacag ggagttggaa aatgaggggc ttgcccgtga gctggttaat aagatacaaa
     4501 atatgcgtaa atcagctgga tttgaggtga tggaccagat caaaatagag ataaaagcaa
     4561 cttctcgggt cgaggaagct gtcactgcat ttgaggatta tatcaagaag gaaaccttgg
     4621 ctaagagcat aagtttttct ttggattcca ccgatacgtt taaagaatgg aatataaatg
     4681 gtgaaaaaac gcaaatctca gtgaatagga taaaaacgat aggaggtaat aagtgaagaa
     4741 aaaagagctg gaaaaatatg agaaactgct tttagctaag cgggctgagc ttctcaagga
     4801 gttgggctta ctcaagaaag cggccatgga taccaccatt aaagattcca ccggggacct
     4861 ctcttcctat tcttatcata tggccgatca gggaacggat gccatggaaa gagagaaagc
     4921 atttctcttc gcttccaaga gcggcaggct tctttatcac attgatgaag ccctgagaag
     4981 aattaaggat aagacctaca gcaagtgtca gcgctgtgga aaacagatca gccaagctcg
     5041 cttagaggca gtccctcatg ctcgtttctg tatcgcctgc aaggaaaagg aagagcgcga
     5101 gaagactgag gagatctaac taaacaagat gattttcctt ctcttgagct tattggtagc
     5161 tctcttggac cagctctcca agggatatat tacctcccat ttccacttag gacaatccat
     5221 ctcccttttg ggggatttcc tcaaaataac ttatatccaa aacccgggtg gagtttttgg
     5281 caccagaatc ggcagcggga acttctatac tttcatctct ctggtcgcta tcctaatcgt
     5341 ccttctggtg tatttcagga tgagaaatca agaagcgact ttcaagatcg ctctcggctt
     5401 aattctgggt ggagccatag ggaacctgat cgaccgcttc cgttttggtg aggtggtcga
     5461 ttttttagat gttgatttct ttaacataaa cctaccccct tttaaattcc tatttttgaa
     5521 attcaacggt ttctatttag accgttggcc agttttcaac ctggctgata gtgcagttac
     5581 agttggcctt gttattttaa tctatcattt ttcctttcag ggggcaaaaa actaagtgtc
     5641 gatgaacgtt aagaccttag agataaagag ttcactcgga gacgagggaa aaagattaga
     5701 tgttttcttg gttgaaaaaa agcttgggct ttcccgctcc cgaatacagg atttgatttc
     5761 ccagcaggaa gttctggtaa acaataaatc tgttaaaccc cattacaaga ttaaaggaaa
     5821 tgaaaccatt ttcatttccc ttccactccc tgttgaatcg gagattaagc cggagaatat
     5881 ccctttggat attttatatg aagataaaga ccttttagtg gtgaataagc cggctggcat
     5941 ggtaactcac ccagcagctg gcaactactc cgggactctg gtcaatgcac tgctatttca
     6001 ttgtaaggac ctttccggaa taaatggagt cttaagaccg ggaattgtcc accgtttgga
     6061 taaagacact tcaggcttgt tggttgtggc taaaaacgat tttacgcata accttctggc
     6121 agaacagctc aaggagagaa agttatttcg tcaatattgg gctttgactt gtggcaatat
     6181 gccaacctgg gaagggaaaa tagaagtgcc tattggaagg tcaactaaag acagaaaaaa
     6241 gatagtggct aacccaccca gaagcaagga gtccattacc ctttatcagg ttctggaaag
     6301 atttgaaata tccgaccttc tgtgtttgaa gcttttgacc ggaaggactc atcagataag
     6361 ggtgcaccta tcttatttgg gaaaaccggt cttaggagat gaaacctacg gaggtaggaa
     6421 gagatggacc aaccagcttg gagaatccca gaaggacttt gcttacagga ttctggaact
     6481 attgcctcgt caggcattgc atgccaaaaa aattggattt gtgcatcctc gcacaaagga
     6541 gtatattgaa atagaatctg acctgccatt ggatatgagc cgagctctga aacttttaag
     6601 aggagagaaa aatgctctta gccattgata ttggaaacac tcatacagtg attggaatct
     6661 tcgtgggaaa ccgtttgaaa aacgattttc gcttctattc ccggcatacc ttgaccgcag
     6721 acgagtgtat tatgcttgtc agccaactct tttctcaaga aaaaattcaa aaagagaata
     6781 tccaaggggt tatcatctgc tcggttgttc ctaatttgac acccattttt caggaagcaa
     6841 gcagaaaata tttgaaggtc aaaccaataa tagttggacc tgatttggat ctgaatataa
     6901 agataaagta tgacctaccc cagcaggttg gagcagatag aatcgccaat gcggttgcgg
     6961 cttatcatct atataaagga ccaactatcg tggttgattt gggaaccgca actacctttg
     7021 acgccatctc tggggatgga gaatatttag ggggtgctat cgctccagga attgaaacct
     7081 cctcagccaa tctttttcat cgggcagctc agctatacaa agtgggcttg gacaaacccc
     7141 aaaaagctat cggaacaaat actacggaga gcttgaagtc aggaatcgtt tttggggcca
     7201 tagggcaaat agatgagata accagaagga taaagaagga attaagggga aaggtttttg
     7261 tggtggcaac tggtggtttg gcaaagctgg tggcaggcga atcaaaaact gttcaaaaaa
     7321 tcaacccaac tctgaccttg cagggtttaa gaataatata caatatggtt acagctaaaa
     7381 agaaaaagag gggataaaaa tggcaaaata agaagtcgag caattaataa tgggaaacaa
     7441 acgaactact ccatattaga gacacgaggt gtcaagtctc tgaatatgaa taaattagga
     7501 atagtatgtc attccgcact tgatgttgaa aacccagtag ttagggcgga atccagtagg
     7561 gttttttgta ggggcctgat ttgcacagca tcttgacgac atcaagcccg tcgggtcgta
     7621 acttgtcccg atttgtcggg ataaatgcga cccctacaat gtcttctgct ttttgtaggg
     7681 gctcgattta tcgagcccgt tcttacgtgg agtacaaagc ttcagcttta tgaagctttt
     7741 ggccccacca aggcgagatt ttcaataaat gcgcccctac aaaacctggg tttctttgtt
     7801 gttttatttt ttaattctct tgattcctgc acgaattccc cgaattcccc gaattctgtc
     7861 cttcgacctt gctcatgacg gtgaggacgt tcggctgagc ggttcgaccg tcctgagtgg
     7921 ctcaccgtcg aaggactcag tcgaagccct tgtcgaaccg tgtcttgaga ctggagttta
     7981 ttccgcgcaa gatgcgggac agggatgaca gcaaaaattg taggggcgta aggcaataca
     8041 cccctacgga ctaacttcaa aaactgcaat agcttatctc aagacacgga attcgtgtac
     8101 atattcctcc tttcatccat agatgattca actattccta aaaaatatta aatttcttac
     8161 tttttttact tgctttaaat caactcaaag tatataatat attttcttta gcactcaaac
     8221 agaccgagtg ctaatctcga gacacagaat tcgtgtaatc gtagatgatt taagattaat
     8281 ccttcggcta tgcttaggat ggtgagttta tcgaaccata aaaaactaaa caaaataagg
     8341 aggtcggtat atgaaggtga agcccttagc ggacagagta ctagttaagc ctttggagac
     8401 tgaggaaact aaaaagggag gaataataat ccctgatact gccaaggaaa agccccagga
     8461 aggtgagata atggaagttg gaccaggcag aataactgaa gatggaaaga aaataccaat
     8521 ggaggtgaag aagggggata agatattgta tggcaaatac tccggaacag aggtaactat
     8581 tgataatgta gactatctga taatgcgcga aagtgatatt ttggcaatag tagggtaaaa
     8641 agaaaaatgt aatcctaagg aaacaagagg aggtttaaga gtatggcgaa gataatagag
     8701 ttcagcgcag aagccaggga aaagctaaaa aagggtgtgg ataagttagc taatgcggtt
     8761 aaggtaactt tgggaccaaa aggtcgaaat gtggttttag atagaaagtt tggttctccc
     8821 acagtaacca aggatggtgt taccgtagct aaagagatag agctggaaga taattttgaa
     8881 aatatgggcg cccagatggt gaaggaggtt gcttccaaga cttcagaagt ggctggggac
     8941 ggaaccacca ctgccacagt cttggctcaa gccatttatc gcgaaggctt gaggaatgtt
     9001 accgctggcg ccaaccctat ggctctgaaa agaggaattg agaaggcagt ggaaaaagtg
     9061 gtagaggagc taaagaagat atccaagcca ctccctggaa agacagaaat tgcccaagtg
     9121 ggtaccatat ctgctaacaa cgataaagag atcggcaatc tgattgcgga tgctatggac
     9181 aaggtgggaa aagatggtgt gataactgta gaggaagcca aaaccaccaa gaccgagctg
     9241 gaggtagttg aagggatgca atttgacaga ggataccttt ccccctattt cataaccaac
     9301 ccggacagca tggaagcgat gctggaagat ccattgattt taattcatga taaaaagatc
     9361 tcggtgatga aagacctgct tcctatctta gagaaaacag ttcaaatggg aaggccaatg
     9421 cttatcatct ctgaggatgt ggaaggtgaa gcacttgcaa ctctggtagt aaataagctc
     9481 agaggaactt tgaaggtctg tgcggttaaa gctcccggtt ttggagatag aagaaaagcg
     9541 atgttagaag atattgcggt cttgaccggc ggcaaggtta tctctgaaga gttgggtcta
     9601 aagttagaaa agactttaga gaacgattta ggcaaagcca agcgggtaac gattgacaag
     9661 gataacacca ccatagttga aggtggcgga aagaccgaag acatcaaagg tagagttaat
     9721 cagataaaaa gggaaataga agaaaccacg tctgactacg acaaggagaa gctccaagag
     9781 agattagcca agttggcagg tggagtggca gtgataaatg tcggtgctac cactgagact
     9841 gaaatgaagg agaaaaaagc cagagtggaa gatgccttac acgccactcg tgccgcagtc
     9901 gaggaaggga taatccccgg tgggggggtt gcctatttga gggcaatccc agctttagag
     9961 aagttaaaat tagaagggga cgaagctatc ggggcatcta ttgttaagaa agctctggaa
    10021 gagccaatca ggttgattgt agaaaatgct ggagtggaag gctcaattgt ggtgaataga
    10081 gtaaagagtg aaaaaggttc ctttggattt aacgctgaga ccgagaagta tgaggacctg
    10141 atcaccgccg gagtcatcga ccccactaag gtggcacgca tagccctgga gaatgcagct
    10201 tccatcgcct cccttttaat caccactgaa gccatcatag ctgacaagcc cgaagaaaag
    10261 aagtcgccaa tgatgcctcc aggtggtggc tatggcggag gagatatgta ttgatgtatg
    10321 aaagctctag attatcatgg taagaagtag ggacagaaca gtgttctgtc cctccgttgt
    10381 ttggtatgtt cgtcattccc acccccgtta tttatgggga taaactccag ctggtatgaa
    10441 ccgcaatggt tcataccagc cgaaatcttt cccgctgtca ttcccgtccc gccaaaggcg
    10501 ggaggaatcc agcttttcta aaaccaaatg gatttctgtc tcaagatgtc gaagacccag
    10561 tccttagggc agaattcgca ttttcacagg aatgacaaaa cttcgtgggc ggaggcacct
    10621 cgccttgcac acgatggaca agcgaccccc aggcctgaaa tttcattata aactcactta
    10681 atgcgtttgt attgataata aaaaaacccc gctttttagg cggggttttt attgggtaaa
    10741 tttttctaaa cttccttatg gctgtggaaa attctccgag acgaacttca gaagcttttc
    10801 tatgcgataa taaagataag tcgaattatc cgcagacaaa tatccaccgc ccgaatactc
    10861 ttcgaagaag gaatgtgcct tttgaccgct ggttagtcct tggtagaagt cactgctctg
    10921 ctgattgaac tcaaactcat ctgcgtctcc acagtcaata taaatagcca tactgtcaag
    10981 cactgccgtc cctctaccat aaatagtctg gacgtcatta gagtctatcc atctattcca
    11041 aacagaggct ataatgtttt gggttgaatc aaaaggtaaa tccacaccat acgtagtagt
    11101 agtaccctga ggagttactt tgaagtagga agtactatca ggattggtcg tgctatgtgg
    11161 actaaaagct gctcccattg caaagaacat cctggtaatc tcatctgggc cttggtaagc
    11221 ttcatttaga gaatcacgat aggcagtgga gtcgaaaaca ccatttacac taaatatgtg
    11281 gtcaatcgca ctattaaggt cccaatcccc aaaagataaa ggaccactca ttgaaccgac
    11341 tatactgaaa atgtcagggt aatccatcgc gattctaaaa gccccatacc cacccataga
    11401 atggccggca atggcacgat aagatttatc ctgatgaaca tagaaacttt tctccacccg
    11461 atacataaaa tcatccacat aaaagttctc gaattttccg tttagacttt gattaggatc
    11521 agaggtgggg ctgggtgagt taacatagaa gctaccagct ggaatggaac cgcccaaata
    11581 actattgcag ttcatcatag cgattatcat aggttgaatc tgacctgtgg ctattagtct
    11641 atctgcggtt tgagctaagc cataagggta gggaaagtca ggggtagtag gaatatagcc
    11701 caaagtagta tccataaagc catgtaggaa ataaagtatt ggatactcag tcgaggcatc
    11761 cgcaccatca tatccaggtg gggtatatac aaatgcattc cctccataag gatttgttat
    11821 taaattatca ctcaaagcgg tattgcgccc caaggttgaa gagtcgacag cttgaaaata
    11881 tacagtccct ttaggataga ctggatctgc tggattcttt ctatcactgc acccaatata
    11941 agcgaaaagg gtaaccaaca agaaaatcag ggcagataac ttatgccaat tcttcatttt
    12001 gctctactcc tcccttgtga gttagaaacg gtaagttaaa gaaaggtgcg tactgtaaga
    12061 catattgccg tattcacctg gcaaattgtc atatactccg tcgttgtttg catcttcaat
    12121 tttatctata gtcttgctgg gggaaagaac gagttgctga ccaagcccca gttcgatatt
    12181 gttctcaaat tcgtaaccta acccgagact aaacccatgc ttgggaccca catccgggac
    12241 tatgatatcc agagtggaat tgggaataag agaagactca aaagagtacc caccacgaat
    12301 agcgagtttg tccttgtaca tgtattcgca tcctaatgct atcttaaaaa catcattcca
    12361 attataagct aatgtgggac tggtggcagt ctcgccaaaa ggatcctttc cggtcagatt
    12421 cacttctaac tcatcccacc gcgaccagcc agtccaactt ccatcaaaag taaaggtcag
    12481 tttatctgag gaaaaatagg caaatcccac acctgcactg gcaggcaggt taagtgttgc
    12541 ctttgccttt ggtattgaag ctaaggttcc tccggtaaag ctggtatcaa cctcaatatg
    12601 attgggaaga taggcagtat aattcatatc tccttccact ttgatgttga ccgggctggt
    12661 gattgataca cccatctgca tcttaggaga tattttatat agcataccca aattaaaccc
    12721 aactccccag ccatccatct ttagcttaga atcgatcggg atattctgca gtggagcgtc
    12781 atatcctgtc tgcgccaatg taaccttagt taatttgaag tctgcccagt taaagctgag
    12841 ccccagacct aaggaaagct tttcggagaa cgctttggcc aaagtcggat gaaaatccac
    12901 aatcattata tttggcccgt aatcatcctt gggataacta accaggttgt catatcccgg
    12961 cagaggctca aaaacactcc aatagtgaga ttccaattca tagctagtat aaaagcccat
    13021 acctacaact atattctgct tcggtaactt gtaaaacccg ctgaaatttg gtaagaaata
    13081 gaacctattc tccggataac gataagcccc attcttgaaa ccgacctcat acaagtcacc
    13141 atacaagaca tccgggttat atttcggcag aaaaagactg gctgaaagag aagtatttaa
    13201 ctcggagctt tccagaaacc ccaagccagc tggattccag aagcacgcgc tccagtcatc
    13261 cgctaatccc ctgaaaccgc cacccaagct ggtcgccttg gttccgattc cattaactgc
    13321 aaaaccagca ccccaggctg ttgacccgat caatatcaag gccaagacaa caaatgaaac
    13381 tgaatttaaa tgtttactcg ccataccctc tccattagtt aaaagttctc gaagaatttt
    13441 ttggtgtctt ttcggagttt agttgattat gaaacataga atcaaaacaa cagatagtag
    13501 gaaccccctt ctctccatta aaaagatagg accagggatt tttcctcaaa aaaacaatta
    13561 gactaaactt actatccccg ctttggtttg tcaatacttt ttttgaaatt tatagtcgaa
    13621 tatatcgcaa gattctgcgc ttatttgaag attgaagttg acacccaaac tctactttaa
    13681 tatcattcat aaaatggaaa agcgccgagg ccaccaggag tatttcaaag agttagttag
    13741 tcttatggct atgctcaggg gaaagaatgg gtgcccctgg gataggtccc agacccataa
    13801 taccttgctt ccttatctca tagaggagac ctacgaggta gtggaagcgg tccaatctaa
    13861 ggactacgaa aggctaaaag aggagctggg agacttgcta ttgcagattg tttttcactc
    13921 tcaaatcgct aaggagaaaa atagatttga tgtttatgaa gtggcaagac agataaatct
    13981 caagcttaga cagagacatc ctcatatttt tgaaaacaag aagaaaatct ccaagcaaga
    14041 ggtgatacaa aactgggagc atataaaact atcccagaag aaggacaaag gagtgttgag
    14101 tggtctgccc aggcaactgc cagggctttt aagagcttat cgcctgcagg agaaagtagg
    14161 aaggttgaat ttcgactgga aaaatgcagc tgatgttctt cctaagctta aggaagagtt
    14221 gggggaattt gaaaaagctt ttaagactaa gaacaaaaga aagatggaag aggaaatggg
    14281 ggatatacta ttcagctggg ttaatctttg tcgccatttg aacatcaatc cggaattggc
    14341 tttaggtaag actgtcgaca aattcatacg caggtttaac tatatcgaga aggcgttggc
    14401 aaaaaagaga attgggtttg atcaagctga cttgcccttc ctggacagtt tatgggaaaa
    14461 agccaagaaa agaaagaata gaaaataaag aaccccccta cggggccttt aaaacccaaa
    14521 attcaaaacc aaatcgagat cccgaaacaa gcctgtcctg gcgccaggcc agggttcggg
    14581 atgacaagaa ccatgaaacc aaaccctgtt gtcatgctga actcgtttca gcatctgctc
    14641 gtttcagcat ctctggtctg tttttttctg cttccaaaag atgcaccttt gatcgccctc
    14701 tacgaaaacg ggggttttca ggtgcaatct tgataaaaaa acccccccgc ttatgaagca
    14761 ggagggtttt tcacttattt agactactta actggaacta tctcgattct tctgttctct
    14821 tgttttcctt ccggggtatc gttggaagca atcggattct ccggacccat tcccttggct
    14881 tcaattcgag cagcatctac ttcttttttc accagataat tcttgactcc gtctgctctc
    14941 agcttggaaa tcctcatatt ggcagccaga gttccagtgg catcagtgta gcccaaaact
    15001 tctattttta catctttata gaagctcaag cgctgagcca gatcatccag ggcggtcatg
    15061 gctggtgcat ctaagttggc actgccagaa gcgtatttga tatgtaaagt tatcttctca
    15121 atggctgcaa ccttggggca acctttttcg tcaactgcta cgccggctgg cgtgttgggg
    15181 cattggtcaa tgccatcaaa tgctccatca gcatctgaat cgatggggca tccgttcaca
    15241 tctatcttag ctcccttagg agtgttgggg cacttatcca gataatccgc aacaccgtca
    15301 ccatcgctat ccataggaca acccttagca tctaccttaa cccctttagg ggtgctggcg
    15361 cattggtcaa tgccatcaaa tactccatct ccatccgaat cgatggggca tccgttcaca
    15421 tctatcttag ctcctttagg agtgttgggg cacttatcca agccatcgta aacaccgtca
    15481 ttgtccgcat ctaaaggaca accattttca tcaaccattg cgcccagtgg tgtgtccggg
    15541 cactgatcta ctttatccgg caccgcatct ttgtcgctat ctttgggctt gccaaaatag
    15601 aaggtagcac caacataagc ttctggaatc aatttcggca gatccaaacc cacttcttct
    15661 ggatcctttt cattcttcaa gctggttaaa acatgagtta agtaatgtgc cttgccgccg
    15721 atgttcaaag ctatgttctc ggtcacataa taatccaaac ccacgccaaa aacggccgtt
    15781 agctcctgat ccttgacttc aaatgaatct agttcacttg tgtccggcca caaaacagca
    15841 tttccatcct tatccttcca attccagaaa gctattcctg caccaccaaa aacgtaagga
    15901 ttaaacttct cctcaggcat aaaatgataa aaagcaactg cctctaccat cattgcatta
    15961 aacttataac cgttggggtc agtcgagaaa gtaaatccag caccatctgt cttgccagtt
    16021 aaatccgctt ctttagtaaa agagtaggta cccatcactc ccactgcaac atttggagta
    16081 aacccatact tcaaaattac actcccctca ggtccaactg cccaagcatc tgaatggtca
    16141 gccaggatgg gtttgaacat agctggatgg acgctcaagc cgaatttacc cgttaaatct
    16201 atggcattgg ctgacactgc catccacacc acaagcaatg ctactaaaag tagcgttaac
    16261 ctcttcactt aaaacctcct tactttaaaa gttaattttc acaaaaaact aatatcaatt
    16321 tctgctaatt aaatcacccc cttacagaat tacaagttta aaattggctt tgggttaaaa
    16381 gccacaattt tgttttaaat aaagcacaac tgttattttt gtcaagcact ttttaaaaaa
    16441 actgggctta ataatatttg aggactttcg ttttggggta gtaatgagct aggatttcag
    16501 aataagataa gcctctattt gccattccga tggcaccggt ttggcacatc ccaattccat
    16561 gaccacttcc cctgccaact gccttggctt ttatcaaaga accatctatt cccctcgtga
    16621 tctctaaatc gaagagggtg ctgggcagga taacatcttt tgttctctta agagcccatc
    16681 tgattttatc tttttctatt ataaaatctt ctttatctgt gcttaccaaa agaaccttta
    16741 ccctgcctga aggagctcta tccaatatct ttaaatccaa aagtcttcca aaatcctcct
    16801 cggtgatacc ctccttttca gatagaaatt cgaataatct ctcttccagc tcttttctgc
    16861 tccattcctc ttcccatctg taattgcccg cccaggtaca gaaatcccca tcctccactg
    16921 acaaaagata gggctcaccc attcctgacc aaacctcttc taggctctct gttcttccac
    16981 cacagttagc atagtaatag gcctgaatca gcttatcttg ataggtcaaa atctttccct
    17041 cggtctggtc caccgcttga tttactaggc tttcttctcc atccatatca gcatacacct
    17101 ggtcataaat ggtagctttc aaatcataaa ccccatcata ctgacccatt tttgacaaag
    17161 cataggtgcg ggcagcgatg gcttgtgctt ttagtgcttc tatttcacct tttttccttt
    17221 tccccatctc agccgggacc acacccttta gataatcctc caaatgaact atattcagaa
    17281 ctacaaggag gtttttgttt tgatccaaga cgatctccaa agctcctcga tatctcttgc
    17341 cgtttaaaga gagccaattc tctctacttt taggttcaaa tatgaccttg ctcagattcg
    17401 actccatagg aggttgattt ttttgtcccc agattatgaa agatgaatca actctcacct
    17461 caatatcggc tgtagagaaa tagttggctg tttgtattcc atttctaaaa catcttatgg
    17521 cgaagttgtt agctggagat actgaaattt ctcgtccttc aagcaattta actgccacca
    17581 ttggttgctt cacctgggag tagaatactc cctgcctcac tgctggaagt tgggcgcagt
    17641 taagaataaa gagtgctaat aaaaacgaaa gggtgatgtt tatatgtctt ctcaaggcaa
    17701 acatattttc cccaatctaa cttccttttt agctccggtc acttccctta agtttccttt
    17761 tttgtttttc atagtcaagt aagctaaaat ggcaaaacag atagcttcta tctggtcagg
    17821 attataaccc agcttttcag atgaaagtac tctaatcgga tgaaacaaca ttgatagcct
    17881 ttttgttata tatcggttgt gactcccgcc accgcataca accaattggt caattttggt
    17941 ttttggtctg acccatctct tataagcatc aaagattgac cagcaagtca actcagtgac
    18001 cgtggcgatg acatcctgtt tgggaatcct cttcgtcccg agaatatatc ttaaattctc
    18061 ttcgaaatct tcccttccag ttgattttgg cggtttcttt ttgaagtagc tgttcctttt
    18121 caattttctc aaaaggtctg gatttacttt ccccttgagt gcgatttccc catttttatc
    18181 atattctttg ctgtatagtt tgttgactaa ggtatccatt aatacattcc caggtccggt
    18241 atcaaaagca aatatatctt tcatcttacc cttcttggga ataagagtga tattggctat
    18301 ccccccaata ttcaaaactg ctcgagcaaa atccctatgc ttgaaaagat ggaaatgagc
    18361 taaaggacaa agaggagctc cctcacctcc gactgccata tcagcctgtc tgaagtcccc
    18421 aatagttaca ctcccagtca gagcggcgat aactgccggc tccccaattt gcaaagtccc
    18481 tcttattttt ttcccacaaa aatttgccac tttgggcaag tgccttatgg tctggccatg
    18541 tgagcctatc aggtctaatc tattcttagc aattcctatt tttttgcata aatcaattac
    18601 cgctcgagca aaactctttc ccaagagcat attcaatctt accaggtcat ccaaattgtg
    18661 atttttatct ttgtaaagct cgaatatctt ctctctgatt gagtctgcat attcataggt
    18721 tttaaatccc aagagtttaa tctttaaatc atccttaact tcttttattc ttactaagca
    18781 aacatctatt ccatcagctg aagtgccaga aattagccca gttaccagca attctttctt
    18841 cttaagcaag gaaaataact tatccatttt tcagtctctt tttgagcttc aagattctat
    18901 caactgaaga tttcactttt cccttttttg tttccccgtc ttttatcccc ttttcaaaac
    18961 tctccagaat ctctttttgc ttttcccacc ttttcgccac aaggattaaa tcatgccctg
    19021 ctaaaaaagc ttttttgcag gcaaaattga tatcccagtt cctggttatt ccgcccatgc
    19081 ccatatcatc tgtgattacc aacccatcaa aaccaagctc tttttttaaa atatctcgac
    19141 atatggtttc agataaagtg gctggaaaag tcggctctaa tttttcacac aagatgtggg
    19201 tagtcattat catctctacc cctgttttta tggcggactt gaaaggaata aaatttattt
    19261 gctcaaattt tgttttctca ttttggtcaa tggataattc cttgtgcggg tcaagagcag
    19321 catttcccaa accgggaaaa tgcttggcgc aggagaaaac cccctgcgat cttatggctt
    19381 ggaccgcaac tttagtaaac cttgctacta atttagcatc atttccgaaa gacctctcct
    19441 tcaaaagctc attttgggga ttagttagaa catccactac aggcgccaga ttagcattta
    19501 tccctaaatt cctcaattct ctggctgtta tggaataagc ctgttcaact cccttttcat
    19561 cttttctttg accaaagaag ctattgctcg gaaaggtcgg aaaatctttg gttattctat
    19621 tcgtctttcc tccctcctgg tctatcatca caaaaagagg gataggagaa attgactgta
    19681 aatctaaaat gacctccctt aattgtcttg aatcctcaat attctccgaa aagattatca
    19741 ctccgccgat tttgtattgt ttgaccaaat cgacgaactc gtctgaagga tataatccct
    19801 tgtatcccaa tacaaaaacc tgtgcccaat tatctgtgaa aggtgtcact cttggattat
    19861 ttagatggtg caaaccaagt tcttacctct acctttagca atatacatag ctagatctac
    19921 tttttgaatc aaaatctcgg agctcgttcc attctccgga taagaggcta tccccacgct
    19981 cactgtcaag ttaaccttaa ggcccttctc ttcgccctca aattgcgtgc tttcaacctg
    20041 cgagcggatt cgctccccga tattggcagc atcgtatttg tcggtttgag gcagaatgac
    20101 cacaaactcc tcccctccgt agcgcgcgaa gatatctaca tctcgaatgc accccttgac
    20161 cacctgcacc aagccgcgca ggatggagtt accgaacagg tgtccatagc tgtcattgta
    20221 tcttttgaag caatcaatat ctatcataat caggcacaaa atctgctcat aacgtcttgc
    20281 gcgcctgagt tcatcctcca gcttttgcac aaaatatcga taattgtaaa ttccagtcaa
    20341 ttcgtcgatg atagtcaatt cttccatctc ttgatggagc atggcatttt caattgccat
    20401 cccggctgaa ctggctaaga tggagaatat cttctcgtcc tgctccagaa aagctcccac
    20461 ccttttggat tcagcattca gcactcccac cactttaccc tgcgatatca ttggaactgc
    20521 aagttcggaa cgtgcattgt ataatccgta aatgtaacgc gggtcagtcc tgatgtcaaa
    20581 cactctttcc ggctttccca gacgcgccac cgtaccggcg attcccgtta gaggaacctc
    20641 ctccggagga gaatatttta tcttatttcc taactccacc ttggctttca aaagcaaaac
    20701 atttctgcgg gaatctaaaa gcaagactga acacatctga tagcccaaaa cctccttgac
    20761 gatgttcagg gtctcatcta tcacctggtc caaatcatga atcgctccca aggttccaga
    20821 ggtttcataa atggtctcga tttgaacttg agatttttct aattccgagg ttctctcgtt
    20881 taaagtatcc aatgcccgaa aaagtttcgc tctggaaagc ttaagatact tggagatggc
    20941 acccactgca aaggcgaaca tccacaagaa cagtattcgt atagccaaat ctccaataaa
    21001 tatatttcct gatccttttt cttgatagat aattagatag agtatagtag aacctaagga
    21061 tagggctaat ccctcgtaga gtcccaagta ataagccgta aaagagatag agagataata
    21121 caagagatag aaattgctct ttactcctcc agtgaagtaa accaaaaaag ttataaaaag
    21181 aatatccgcg atggaggaga aaagcaacaa tttaccatgc tttttggtct tgcgagaaat
    21241 tatcgagaat accagaaggt gtaacagaaa tacaaatgcc aagatcatac ccacattgta
    21301 ctccgagccc ctataggaga aggaaagcca gatagagaat cctaagaaga gcaccgaacg
    21361 cacaagtgca aacatattat tgaagctgga gagccgctcc aatagaacgc ctttgtcctg
    21421 tgatatcata gtttgctgct caaaaggtat aattttccct ttgggtgtct ttagcttaag
    21481 tcacacccct actattctta tatcggcaga aaagacaacg ctttaacagg acattccaag
    21541 ccgaaagtat ttcaaaaaat ctaaaaaatt cgactcaaat ttatcgctca gttttatcac
    21601 ttatcttgaa tggttttttt cttaacttaa tagcttacca aaagataaat catgctttat
    21661 acagtgtctt gtcaacaaaa tattactgca ttttttgtca ttctgacccc gcctttgtgg
    21721 gggaagaatc tcacttttat acgggaatcg agacccttca cggagcctgc cctgcaccag
    21781 aacggttcag ggcctaccct aaagtgaaac gaatgggttc agggtgacaa tattgtcaca
    21841 ccattaatta gttgacaaag caatatcgca tagggatgaa ttccctatgc tacgagatat
    21901 ctgctcgtag gtgtgggaat ttatcccaca ccgcgctatc acagtttctg tcaggaccta
    21961 ctcttgacag ttgtaggcca tccatttggg tcgcctacat ttttgcgcta cgaatttcct
    22021 ttacaaactc ctgttggttc gggaattgga attttaccct acggggaatc ccggtgaacc
    22081 gaaccgtgaa gccatgcggt aaatccccgt cacgggtctt gacgacaatc cccgcataaa
    22141 cagatcactt cgtcgtccca atggctcctc gctatgaacc atatatggtt catagctcgt
    22201 aatgacaaaa cgtatcctgg ttgccattgc cagtcccgat gaaatcgggg cgtggcaatc
    22261 tctattgttt ccgtgtttta gaattcggct ctcattccta tcttcacgag gcggggagtt
    22321 ccggctagat taaggaaaag actcaactct aaaatacttt attccccctg aaaggtagag
    22381 ccggacaaaa acctttctgc tggcactttg aacaagccgc aaatctcaag attagtttct
    22441 caataaccag aagcgaaaca taaaccaaaa gaagcaccaa gctggcgagg tttctgtatc
    22501 tgatcagaag aattattccc agaagaagcg gaaaaaacca taaagggtaa agcagaatgg
    22561 cataaggata cttgcttaca tatttctctt cgtccccctt attgaacaga tatgcggcaa
    22621 gttttcccca accggtatta caccatctgt tatgataata gcagtaaacg caacgcgaca
    22681 agggaagaac cagggttaaa ccgaaaatgc tgtaaaggat atagagccaa gctaaaattt
    22741 gattcagctg atagatgata tatattccta aggcgaaaat gattgacaaa accatccaac
    22801 gaagtatgat tacatgccaa gggtaagttt tacaggaaaa tggctcttga gcagattcgt
    22861 tcatgtgtct ttctcctttg agaatttatg aaaccaaaaa gaatatctta cgtaatttaa
    22921 ccaggataat aatatcatct aaaaggagtc attatgtcca aaaaaatctt ctctattgtt
    22981 atatttgcct tgtttcttct gacagcttgt ttcaaattta gtttggctac ccaattcagg
    23041 gctggcgata acctcatttt tgcgcctgat aagaagattg atgacgacct catggctgcc
    23101 ggcgggaaga tcaaattcaa tggagttctc tccggagacc tgttagtcgc tgccagctca
    23161 gtggctcaaa atggaaccat tcaaggcagc ttcatggctg cggcacaaca aatcgacatc
    23221 tcgggtccaa ttgatagaag ctccaggact ttttctcaat acgtaaacat aagctcatcg
    23281 gtcaatcaaa acgttatggc tttttgccag cgctgtgacc tgaaatcaac tggatatgta
    23341 ggcaaggatt tccacgcctt ctgcagtgag ctaaacgtcg atggcaaaat taaaggaaat
    23401 ttgaaaggct acaaccagac tgtcaccatc tccggtacga tcgagggaga cgtaagcctc
    23461 gtggccgaga gaatcgttct tatgcccact gcgaaaatca atggaaatct caactataaa
    23521 agcccaaatg aagctgacat ccaaaaggga gcaagcgtcc tcggtaaaac ccaatggaag
    23581 aaaacggaac cgaaaaagaa aggaactgct cctggaagat tcattcttaa cagattcttg
    23641 agctttctgg gggttctcat tcccgggtta atcctaattc cgattttcaa agagcagact
    23701 caacgcatca aaactaacct taaaaattcc ccttttaaga gtttggcaat aggctttatc
    23761 ttcttaatct gcatgctggt gatcttcatt atgttcttta tgctctcttt tgtcatcatc
    23821 ggcatacctt tagcggtgtt atcttttatg gtcatcctat ccatgctttt cctctgcaag
    23881 atagcagtag gagtagcttt gggggattgg ctgttatctt tcttgagcag taatgggaac
    23941 gtgaatacca tccttgctct actggttggg cttattatcc tgactctttt aactagtatc
    24001 ccttacttta ttggcacact attttatctt attattctgt tttccggaac gggcgccata
    24061 atctatgtga aaagaaaggc aagttagatt ttggcccttc agtatttaat gtggaagttg
    24121 ttgttctatt atgcccttct tttggagtgt tcggtctcaa gatcttgaga cttcagccga
    24181 accaagggtc tgcctaacgg ttcgactttg cccttcgaat tcctcagggt ggtgagcgaa
    24241 gtcgaaccac tcaccgtcct gagcttgccg aaggaaggca gatctcaaga tgcatctcaa
    24301 gacccagtct caagatgcag aattcgcaaa ttcggggaat tcgcacactc cagatacact
    24361 gaacaatgtc ttgctccgtc attcccgcct ctgcgttcgt aggggtaaac tccaggggta
    24421 ggtaatggct gcgccattac catttcgggg caagtgcccc tacccgctcg ggcgatagat
    24481 atgctttgcc ccaaattatt aagaatgatc ctaagttttt aagctccaca acatttttta
    24541 tatttctttc cactgccaca aggacagggg tcgtttcttc caacttttgc cttctcccgg
    24601 cgaacagtct tgactttagt tgccccttct ccttccggtt gagccagcgc ataatccatt
    24661 ccacttacag attgatgaat tgtttttatt tcttctcttt cttcttttct ggatttaact
    24721 gattggtctg caatttgcaa cttaaatatc agctcaacta cttcttggtc gattgtctct
    24781 atcatctggc tgaacatggc aaaagcttct ttcttgtatt ctattagtgg gtccttttga
    24841 ccataagccc tcaagcctat gccggttttg agctggtcca tctcatatag atgttctttc
    24901 catttatcat ctatcaccct gagcatagca tatctttcca attcacgcat aagttgagac
    24961 cctattgtct tctccttatt ggtgtagact tccatagcta tctgccttat tttttctttt
    25021 agagcatcct gggttaagtt gggaatatcc ttttcttcga atcttaaatc caccaggaag
    25081 gtctttaaaa gttcgcttct caatccagtt agattccagt tctccggata ggtattcaag
    25141 ggggcgtatt gctccacttt ggaatctacc acttggccaa ttaaatcaac tatctcctcc
    25201 ttgaagtctt gaccctccag ggcagccagt cttctgtcat aaatcacttc cctttgctga
    25261 ttcatcacgt cgtcatattc caacagatgc ttgcgaatag cgaagttctg tacctccact
    25321 ctcctctggg ctctctgtat ggctttggtt accattggat gctgtataac ttcaccctcc
    25381 ccaactccaa gcctatccat tactgttgca atccggtctg aaccaaaaag ccgcatcagg
    25441 tcatcttcca gagataaaaa gaagcgggat gaaccggcat ccccctgcct tccgctcctt
    25501 ccccttagct gtctatctat tcttctggct tcgtgtctct ccgttccaat tatcctcagg
    25561 ccacacggaa cgttttcata acaatccaat tcctctaaat gaggacagcc atcttctcgc
    25621 tccggggtaa taaggcggca cttcttgctt ttcaccacac tttcgcccag cttgatgtca
    25681 gttcccctgc ctgccatatt ggtggcaatg gttaccgcgc ccgatcttcc tgcctgaaca
    25741 atgatctccg cttccttttg atgatatttg gcattaagca ccgaatgggg gattcctttt
    25801 cttttgagca ttcgcgataa tgtttccgag acttcaactg aaatagtgcc tactagaact
    25861 ggcattcctt gcttgtggca tgcctctatc tcatctatga tggcattgta tttttcccga
    25921 cgggtgcgat agatcacgtc atcgtaatcg attcttctga ctgactcatt cgtgggaata
    25981 accactacat ccaatttata gatttcccat aactcagccg cttcggtctc agctgttccg
    26041 gtcattcctg ccagtttacc atataagcgg aagaagtttt gcaaagtcac tgtggcgaaa
    26101 gtctgagttt caccctcaac tctcacttcc tccttagctt caatagcttg atgcagtccg
    26161 tcgctatagc gtcttcccgg aaggagccgg ccagtgaact catccacaat catcactttt
    26221 ccatcctgca ccacatattc cacatctttt tcaaacaagg catatgcctt gagaagctgg
    26281 gttatgctgt ggttcttgtc gctgcgttca gcatacagtt tgtagagctc ttcctttttc
    26341 tcctgcttga ctttttccga cagggtagca tctgaatcta tcttgtgcag tccctcggcg
    26401 atatccggaa taacaaacag ggcttggtcg ctggggtcta aaagagacct tcccatctcg
    26461 gtcatattga tgctgttttc cctttcgtca atggcataat acagcatctc atctatctca
    26521 ttgattttct tatctcttaa gtagtccatc tctacggtgg taatcaattt cttcattccg
    26581 gtctctttta acaacttcat aaatttctta tttttgggag atcccctctg aaccgccaag
    26641 agttttactc cagcttcgta agtttttcct tcccctaatg acttctccgc ctcggaggtg
    26701 aactgattaa ccaaaaccgc ctgcttttta accaatttat caactaatgg cttcatctcg
    26761 ctatatcttt gggtagtgga gaagctcacc ggaccggaaa taatcaaggg agttcgagct
    26821 tcatcaatca ggacgttgtc cacctcatcg gttatggcat aattgaaccc tctttgcacc
    26881 cggtcctcaa cccgtattgc catattatct ctcaagtagt cgaatccgaa ttcgttattg
    26941 gtgccgtagg tgatatcaca atcgtattgc tcttttcttt tttgggaatc catatcattt
    27001 tgaatacacc caattgttaa acctaagaac ttataaatct cacccatcca ttggctatcc
    27061 ctttttgcca gataatcgtt gactgtaact aagtgcaccc cttttccggt gagagcattg
    27121 agatagatcg gcatagtggc aaccagggtt tttccttctc cagttgccat ctccgcaatc
    27181 ttccctttat gcaaaaccac tgctccaact aactgcacat cataaggcac catatcccat
    27241 tcgatttcaa ttccgcacac ttcccatctt ttccccaaaa gcctgcggca gacatccttt
    27301 acaaccgcaa aagcttcagg caggatatca tccaaagtct cttctttggc tagcctctct
    27361 ttgaattcaa tagtcttggc tttgagctgg tcgtctgaca gtttctgata ttcctcgtaa
    27421 tgttggttca tcagagcaac gatgggcatt atctttttga tgtcgcgctg gtgtttggag
    27481 ccaaagattt tggttaaaat attcccgaac ataattctcc ttaaacttta tcgtaaaata
    27541 atacgaaatg tgcttactat aagcaaggaa aaactaggcg gaagtatccc caattttttg
    27601 aaaatgtgtc aggatgtttc ccttaagaca ataagacatc ttcattagtt t
//
LOCUS       DASWLN010000163         5340 bp    DNA     linear   ENV 20-FEB-2024
DEFINITION  MAG TPA_asm: Candidatus Zixiibacteriota bacterium isolate
            SMAG_U2949 k141_1216524, whole genome shotgun sequence.
ACCESSION   DASWLN010000163 DASWLN010000000
VERSION     DASWLN010000163.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37960594
            Sequence Read Archive: SRR10376091
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Candidatus Zixiibacteriota bacterium (soil metagenome)
  ORGANISM  Candidatus Zixiibacteriota bacterium
            Bacteria.
REFERENCE   1  (bases 1 to 5340)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 5340)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (26-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/12/2024 08:28:26
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 1,440
            CDSs (total)                      :: 1,416
            Genes (coding)                    :: 1,412
            CDSs (with protein)               :: 1,412
            Genes (RNA)                       :: 24
            rRNAs                             :: 1 (16S)
            partial rRNAs                     :: 1 (16S)
            tRNAs                             :: 21
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 4
            CDSs (without protein)            :: 4
            Pseudo Genes (ambiguous residues) :: 0 of 4
            Pseudo Genes (frameshifted)       :: 0 of 4
            Pseudo Genes (incomplete)         :: 3 of 4
            Pseudo Genes (internal stop)      :: 1 of 4
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..5340
                     /organism="Candidatus Zixiibacteriota bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_1216524"
                     /isolate="SMAG_U2949"
                     /isolation_source="soil"
                     /db_xref="taxon:2053527"
                     /environmental_sample
                     /country="USA: Oregon"
                     /lat_lon="42.99 N 123.63 W"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            <1..586
                     /locus_tag="VGB16_05430"
     CDS             <1..586
                     /locus_tag="VGB16_05430"
                     /inference="COORDINATES: protein motif:HMM:TIGR04183.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0031240 - external side of cell outer
                     membrane [Evidence IEA]"
                     /codon_start=2
                     /transl_table=11
                     /product="T9SS type A sorting domain-containing protein"
                     /protein_id="HEX9917161.1"
                     /translation="ARIHGSLTGLSLPIPSGYYVNAETDSWPNGYHSINAEVNGTTGA
                     FDLYVCDGNWTFYPPSIPNYSSPSSQNLTISEIPDTLRTIVFSDYSGVDDNQGSGALP
                     KKFALEQNHPNPFNLETTIEYALPKEGQVKIAIYNVLGQKIRTLLDRREPVGCRRIMW
                     DGKNDRGEIVSSGIYFYRIETEGFIQAKKMLLLK"
     gene            748..1758
                     /locus_tag="VGB16_05435"
     CDS             748..1758
                     /locus_tag="VGB16_05435"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_014857055.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016491 - oxidoreductase activity
                     [Evidence IEA];
                     GO_function: GO:0016651 - oxidoreductase activity, acting
                     on NAD(P)H [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="Gfo/Idh/MocA family oxidoreductase"
                     /protein_id="HEX9917162.1"
                     /translation="MNRIKTGVIGTGHLGRFHAKVYSQLDGAELVGVYDIDQDKAKKL
                     AIELGTNCFSELDQLLKKIEAASLVVPTSSHFEVGKEILESDVHLLIEKPIAQTVQQA
                     EALITLAKEKNLTLQVGHIERFNPAFLAIENLKLNPKFIESHRLALFNPRGTDVAVVL
                     DLMIHDIDLILSLVKSELRDIQAASISVITESEDIANARLTFENGTVANITASRISAN
                     NMRKIRFFQKNSYISLDLLRKEAEIYRLVDLKDYSLKEEESSTIIGKIPTEQIGKTIL
                     YEKPKIEDRDMLTLEISSFLEAVRNRKKPKVTGEDGKRALEVALKIIEKSSQHRKGMK
                     EI"
     gene            1755..2894
                     /gene="lpxB"
                     /locus_tag="VGB16_05440"
     CDS             1755..2894
                     /gene="lpxB"
                     /locus_tag="VGB16_05440"
                     /EC_number="2.4.1.182"
                     /inference="COORDINATES: protein motif:HMM:TIGR00215.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008915 - lipid-A-disaccharide synthase
                     activity [Evidence IEA];
                     GO_process: GO:0009245 - lipid A biosynthetic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="lipid-A-disaccharide synthase"
                     /protein_id="HEX9917163.1"
                     /translation="MNSSKEILIIAGEASGDLYGSSLVAELKKLNSEIDFFGMGGNQM
                     KEQGVRLSFHIDQLGFMGIAEVLKNLRFIRQVGKKMVSLLDQRKPDLVILIDYPGFNL
                     RFAKQIKKRRITLVYYISPQIWAWGGKRINKIKKYVDKMIVILPFEKEIYQKAKVDVE
                     FVGHPLLDIVKPTLSQEDFKRKFDIRKNQLLVGLLPGSRWQEVEKILPPMLRAVEISK
                     RKLKNITCAIGLAPTIPSQGLEDLIGPFESKPKIVENLTYDLMKYSDLLLVTSGTATL
                     ESAILETPLIILYKTHFLTWLIGKNLVKLPNIGLVNILAGKRIVPEYLQKDVQPEKIA
                     SQMEEILENREKHKKIKEDLKMVKQKLGEVGASKKTAQIINQMLS"
     gene            2896..3555
                     /locus_tag="VGB16_05445"
     CDS             2896..3555
                     /locus_tag="VGB16_05445"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_020250696.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="lysophospholipid acyltransferase family protein"
                     /protein_id="HEX9917164.1"
                     /translation="MKRFLDKLTLFLIPIIGAFLILLLGKTWRIKWVGRENILPARKT
                     NQKVIYAFWHGRMLVLSFSHRWQKVCVLISQHRDGEFISRIINLLGFASVRGSTTRGG
                     TKAIFQMANKNLEGYNVAVTPDGPKGPKYIAQPGAIYIAQRSGTPIIPVSNSAKKRWT
                     LKSWDEFIIPKPFSEVVIILGDPMHVDAQASLEEIEEKREELEEKLVALTSQANNYFV
                     K"
     gene            3552..4829
                     /locus_tag="VGB16_05450"
     CDS             3552..4829
                     /locus_tag="VGB16_05450"
                     /EC_number="2.4.99.12"
                     /EC_number="2.4.99.13"
                     /inference="COORDINATES: protein motif:HMM:NF016311.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016740 - transferase activity [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="3-deoxy-D-manno-octulosonic acid transferase"
                     /protein_id="HEX9917165.1"
                     /translation="MIYFIYNVFFTLIWLLLLPYLLIRGLWGKHGVVERLGFLPRTWR
                     ETFKKNQVIWIHAASVGEVKMLPAIIPRLKDEFPQTIIVVSVMTRTGKKEAQKNLKDV
                     QFIFHPPLDLLWSTRKAARAINPSLFILTETELWPNLIREVKKRDSKIALINGRMSNK
                     SFPNYKRFKFFFSKVLFFVDAFCMRSQLDAKRIISLGAPPHKVNVVGNIKFDSIFFQE
                     ENVDKKQVDAFSQIVSRKKIIVAGSTHPTEEEMILRTFKKLIFSHSDLILIIAPRHLN
                     HLNELEKDLTQFGLNSIRRSELNQNLDPQCEVIILDTLGELNQFYSLAKIAFVGGSLV
                     PKGGHNLLEPARFSVPVLFGPYVDNFQEMAVLLRTSGGGIQVKDETELYLKMDELIID
                     ESKRKILGQKAKEAISQQASAAERTAQILSRVW"
     gene            4786..>5340
                     /gene="lpxK"
                     /locus_tag="VGB16_05455"
     CDS             4786..>5340
                     /gene="lpxK"
                     /locus_tag="VGB16_05455"
                     /EC_number="2.7.1.130"
                     /inference="COORDINATES: protein motif:HMM:NF014645.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0009029 - tetraacyldisaccharide 4'-kinase
                     activity [Evidence IEA];
                     GO_process: GO:0009245 - lipid A biosynthetic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="tetraacyldisaccharide 4'-kinase"
                     /protein_id="HEX9917166.1"
                     /translation="MPQRGPLKFYPGYGSLQIFLLILSQFYYLIIKIRDFFYTTGIFK
                     RYKLNAKVISVGNITWGGTGKTSFVSFLAKNLVENNYKVAILIRGYKRKEKKMITIVP
                     GIKDLDWQKVGDEAYLLASQLDSAPIIVNKNRVESGKEAIDKYSSDFLVLDDGFQHQK
                     LMRDLDIVMIDASDPFGNGKLIPAG"
BASE COUNT         1689 a         1015 c         1190 g         1446 t
ORIGIN      
        1 tgctcgtatc catggcagtc tgaccggtct ctctttacct attccttcag gttattatgt
       61 caatgcggaa acagattcct ggcctaatgg ctatcatagt attaatgcag aagttaatgg
      121 aactaccggc gcttttgatc tgtacgtctg cgatgggaac tggacttttt atccaccttc
      181 cattcctaat tatagctcgc catcttcaca gaatcttact atctcagaga ttcccgatac
      241 cttaagaacc attgtttttt ctgattattc tggggtcgat gataaccaag gatctggagc
      301 tctgcccaaa aagtttgcct tagaacaaaa tcatcctaat ccctttaact tagagacaac
      361 catagaatat gccttgccta aagaaggtca ggtgaagata gccatctata atgtccttgg
      421 acagaagatc agaaccttac ttgaccgaag agaaccagtt ggttgcagaa gaataatgtg
      481 ggatggaaag aacgacagag gagaaattgt ctcaagtgga atctatttct acaggataga
      541 aacagagggg tttattcaag cgaagaagat gttgttgctg aagtaagtta aaggtcaagt
      601 tctaaactta ctggaccgcc aagggaaact cttggcggtc ttttgtattg agccaaatct
      661 ttgtttgact gtttcttaag tttctattat atttccttaa aagatgacca ctgctctatt
      721 ggtggtcttt gttttagaaa tcaaaatatg aacagaataa aaacaggggt aattggaaca
      781 ggccatctgg gcagattcca tgccaaggta tattctcagc ttgacggagc tgaactggta
      841 ggagtgtatg atatagacca agataaagcc aaaaagctgg caatcgagct tggcactaac
      901 tgtttttcag aattagacca gcttctaaaa aaaatagagg cagccagctt ggtggtgccg
      961 acctcatccc actttgaagt gggcaaagaa attttagaaa gtgacgttca tcttctaatt
     1021 gaaaagccca ttgctcaaac tgtccagcag gcggaagcac tcataacttt agccaaagag
     1081 aaaaacctaa ctctacaagt agggcacatt gaaaggttta atcctgcttt cttggcaatt
     1141 gagaacttga agttaaaccc caagtttatc gaatcccacc gcttagcttt atttaacccc
     1201 cggggaacag atgtggcagt ggttctggat ttgatgattc acgatatcga tttaatcctg
     1261 agtttagtca aaagcgaact tagagatatc caggctgcca gcatctcagt cataactgaa
     1321 agtgaagata tagccaatgc ccgtttaacc tttgaaaatg gaactgtggc caatatcacc
     1381 gccagccgca tctccgccaa taatatgcgc aaaataagat tcttccagaa gaacagttat
     1441 atctccctgg atttgctacg taaggaagct gagatttacc gtctggttga cctgaaagat
     1501 tactccttaa aagaggaaga atccagcacc attatcggaa agataccaac tgaacaaatt
     1561 ggcaaaacca tactttacga aaagccaaaa attgaagatc gggatatgct aactttggaa
     1621 atctcttctt ttttggaggc ggtaagaaat aggaaaaaac ctaaggttac aggtgaggat
     1681 gggaaaaggg ctttggaagt tgctctgaag ataattgaaa aatcaagcca gcacaggaaa
     1741 gggatgaagg agatttgaac tctagcaaag agatacttat tatagcaggc gaagcctccg
     1801 gggatctata tggctcaagc ctggtggcag aattaaaaaa gctcaactct gaaattgatt
     1861 tttttgggat gggtggtaat caaatgaaag aacagggggt taggctatcc tttcatattg
     1921 accagctcgg ctttatggga attgcagagg tactaaagaa cttaaggttc atccgacagg
     1981 tgggaaaaaa gatggtttcc ctcttagacc agcgaaaacc tgatttggta attttgatag
     2041 attatcccgg attcaatttg agatttgcca aacaaatcaa gaaaagaagg ataaccctgg
     2101 tttattatat cagcccacag atctgggctt ggggcggaaa gagaataaat aagataaaaa
     2161 agtatgttga taagatgata gttatccttc cctttgaaaa ggagatatac cagaaagcaa
     2221 aggtggatgt ggaattcgtg ggtcatcccc ttctggatat agttaaaccc actttaagcc
     2281 aggaggattt caaaaggaaa ttcgatattc gaaaaaacca attgctggtt gggcttcttc
     2341 ctggctcgcg ctggcaggag gtggaaaaga ttctcccccc aatgctccga gcggtcgaaa
     2401 tatccaagcg aaagctaaag aacataacct gcgctatcgg cttagccccc actattccca
     2461 gccaaggctt agaggatttg attggtcctt tcgagtccaa gccaaagatt gttgaaaact
     2521 taacttacga tttaatgaaa tattcggatc tgcttttggt aacctctgga actgcgactc
     2581 tggagtctgc tatcttggaa acgccgctta ttattctgta taagactcat ttcttaactt
     2641 ggttaattgg caagaatctg gttaaattgc ccaatatcgg cttggttaat atcctggcag
     2701 gaaaaagaat tgtgccggaa tatctacaaa aggatgtcca gcccgaaaag atagcttccc
     2761 agatggagga gattttggag aacagggaaa aacataagaa aatcaaagaa gacttaaaga
     2821 tggttaagca gaaattggga gaggtgggtg cttctaagaa aactgcccag attataaatc
     2881 aaatgctttc ttaatatgaa aagatttttg gacaaattga ctctgttctt gattccgata
     2941 atcggagctt ttctcattct ccttctgggc aaaacctgga gaataaaatg ggttgggagg
     3001 gaaaatattc ttcccgcaag gaaaacaaat caaaaggtga tatacgcttt ttggcacgga
     3061 aggatgctgg tgctttcttt ctctcatcgc tggcagaagg tctgtgtctt aatcagccag
     3121 caccgggatg gggaattcat ttccagaatc ataaatttgc ttggatttgc ttcagttcgg
     3181 ggttcaacca cccggggtgg aaccaaagcc atatttcaga tggcaaacaa gaacttggag
     3241 ggatacaatg tagcggtgac tccggatggt cccaagggac caaaatatat agcacaaccc
     3301 ggagcaatct atattgccca aagaagcggc acacccataa tacctgtctc caattcagcc
     3361 aaaaaaagat ggactctcaa aagctgggat gaatttatca tacccaagcc attctccgaa
     3421 gtggttataa ttctaggtga tccgatgcat gtggatgccc aagcctcctt agaagagata
     3481 gaagagaaaa gagaagaact ggaagaaaaa ttggttgcct taacctcgca ggcgaataac
     3541 tattttgtaa aatgatttac ttcatatata atgtcttttt caccttaatt tggcttttgc
     3601 ttcttcctta tcttttgata aggggcctat ggggaaagca tggggtggtt gaaaggttag
     3661 ggtttctgcc cagaacatgg agggaaacct ttaagaaaaa tcaagtgatt tggattcacg
     3721 ccgcctcagt gggtgaagtt aagatgcttc cggctataat accgagactg aaagatgaat
     3781 tccctcaaac tataatcgta gtatcggtta tgactcgcac tggaaaaaag gaggcacaga
     3841 agaacctgaa agacgtgcaa tttatttttc atccgccttt ggatttgctt tggtcaacca
     3901 gaaaagcggc aagggcgatc aatcccagtt tgtttattct gactgaaacc gagctttggc
     3961 ctaatctgat aagagaagtt aagaaacgtg attcaaaaat tgccttaatc aacggcagga
     4021 tgtccaataa atctttcccc aattacaagc gatttaagtt tttcttctct aaagtccttt
     4081 tctttgtgga tgccttctgt atgcgcagcc agctcgatgc taaaagaata atctctttag
     4141 gagcccctcc tcataaggtg aacgtggtgg gaaatatcaa attcgattca atctttttcc
     4201 aggaagaaaa tgtggacaaa aagcaagtgg atgctttctc ccagatagtc tcgagaaaaa
     4261 agatcattgt ggctggatct acccatccga ctgaagagga gatgatttta agaactttca
     4321 aaaaacttat tttctcccac tcggatttga tcttgataat tgccccccgc catctcaacc
     4381 atctaaatga actggaaaaa gatctgaccc aatttggatt gaactccata agaagaagtg
     4441 aattgaacca gaaccttgat ccgcaatgtg aagtcataat cctggatact ttgggtgagc
     4501 tgaaccaatt ctatagcttg gcaaagatag cttttgttgg aggaagcctg gtgcccaaag
     4561 gcgggcacaa tcttctggag ccggcaagat tttcagttcc ggtgttattt gggccatatg
     4621 tggataattt tcaagagatg gcggtcttgc ttagaacctc tggaggagga atccaggtca
     4681 aggatgagac cgagctttat ctgaaaatgg atgaactcat aatagatgag agcaagagaa
     4741 aaatcctagg tcaaaaagcc aaagaggcta tcagtcaaca agcaagtgcc gcagagagga
     4801 ccgctcaaat tctatccagg gtatggtagc ttgcagatat ttctcttaat attatcccaa
     4861 ttctattatc ttataatcaa gattagagat ttcttttaca caactgggat atttaagaga
     4921 tataaactga acgctaaggt tataagtgtg gggaacataa cctggggcgg aaccggaaaa
     4981 acaagcttcg tatcttttct ggctaagaat ctagttgaaa acaattacaa agtcgcaatc
     5041 ctcatccggg gctataaaag aaaagaaaaa aagatgataa ctatagttcc cggcataaag
     5101 gatttggatt ggcaaaaagt tggggatgag gcatatcttt tagcctcaca actcgactcc
     5161 gcgcctataa ttgtgaacaa gaatagggtc gaatctggca aagaagcaat agataaatac
     5221 tcttctgact ttctggtttt agatgatggg tttcaacatc aaaagctgat gcgggattta
     5281 gatatagtta tgatagatgc ttctgacccg tttggaaatg ggaaactcat tccagctgga
//
LOCUS       DASWLN010000164         2891 bp    DNA     linear   ENV 20-FEB-2024
DEFINITION  MAG TPA_asm: Candidatus Zixiibacteriota bacterium isolate
            SMAG_U2949 k141_126565, whole genome shotgun sequence.
ACCESSION   DASWLN010000164 DASWLN010000000
VERSION     DASWLN010000164.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37960594
            Sequence Read Archive: SRR10376091
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Candidatus Zixiibacteriota bacterium (soil metagenome)
  ORGANISM  Candidatus Zixiibacteriota bacterium
            Bacteria.
REFERENCE   1  (bases 1 to 2891)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 2891)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (26-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/12/2024 08:28:26
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 1,440
            CDSs (total)                      :: 1,416
            Genes (coding)                    :: 1,412
            CDSs (with protein)               :: 1,412
            Genes (RNA)                       :: 24
            rRNAs                             :: 1 (16S)
            partial rRNAs                     :: 1 (16S)
            tRNAs                             :: 21
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 4
            CDSs (without protein)            :: 4
            Pseudo Genes (ambiguous residues) :: 0 of 4
            Pseudo Genes (frameshifted)       :: 0 of 4
            Pseudo Genes (incomplete)         :: 3 of 4
            Pseudo Genes (internal stop)      :: 1 of 4
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2891
                     /organism="Candidatus Zixiibacteriota bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_126565"
                     /isolate="SMAG_U2949"
                     /isolation_source="soil"
                     /db_xref="taxon:2053527"
                     /environmental_sample
                     /country="USA: Oregon"
                     /lat_lon="42.99 N 123.63 W"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            <1..399
                     /locus_tag="VGB16_05460"
     CDS             <1..399
                     /locus_tag="VGB16_05460"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEX9917167.1"
                     /translation="EDKWGFTGKERDQDQRTIKAKIKCFSTYALGQDTIPPSIRIIRP
                     KEKQKLKVKRPEIVIKIKDDFSGIEDDRDIEAYLDGEWLIPEYDIDNNILRTKPHFDL
                     GYGEHTLTVQVKDRMGNTSSAKSRFEVISQ"
     gene            396..1064
                     /locus_tag="VGB16_05465"
     CDS             396..1064
                     /locus_tag="VGB16_05465"
                     /EC_number="3.4.21.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_018206556.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004252 - serine-type endopeptidase
                     activity [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="rhomboid family intramembrane serine protease"
                     /protein_id="HEX9917168.1"
                     /translation="MIPLKDDIPSYRPPIVTIGLIVANCVIFFLQLSSGSQGFEMLIS
                     KYGAIPYEITHLGTVNSHPAFPIGLTLFSSMFMHGGFFHLVGNMLYLWIFGDNVEDRL
                     GHIKFFIFYVTSGLIASFSYIITAPGSNVPMIGASGAISGVLGAYLLKFPRARISTLI
                     FFGFFVRMVKIPAFLVLGFWFILQLLSGLPSLGAEASGGVAWFAHIGGFLGGMLLLKL
                     FGGL"
     gene            1211..1519
                     /locus_tag="VGB16_05470"
     CDS             1211..1519
                     /locus_tag="VGB16_05470"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEX9917169.1"
                     /translation="MKYKTEAKKKARCAKCGKRLKKGGLKYNIRVEVASDFDGYLEDY
                     SKKPLDYLENRLKILEEDLKDRKELELEEDVYIERSFLVCGECRDRFVKILREFEGEE
                     "
     gene            1516..>2891
                     /gene="dnaE"
                     /locus_tag="VGB16_05475"
     CDS             1516..>2891
                     /gene="dnaE"
                     /locus_tag="VGB16_05475"
                     /EC_number="2.7.7.7"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_015326106.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0009360 - DNA polymerase III complex
                     [Evidence IEA];
                     GO_function: GO:0003887 - DNA-directed DNA polymerase
                     activity [Evidence IEA];
                     GO_process: GO:0006260 - DNA replication [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="DNA polymerase III subunit alpha"
                     /protein_id="HEX9917170.1"
                     /translation="MKHSNFVHLHIHTHYSLLDGACRIDDLVNSAREYKMPALAITDH
                     GNMFGIVEFYKKMVSAGIKPIIGCEVYVAPVSRHKKEPVRGFPDAGYHLILLVKDSVG
                     YKNLIKLATIGYLEGFYHKPRVDEEVLRKYSQGLVALSSCLKGEIACLILAEKYDEVK
                     KAANKYVEIFGENNFYFELQDHGLDEEKKVARELVKLSKEMLIPLVATNDCHYLKQED
                     YLAHDALLCIQTGRKVTDEDRMRFVSDQLYFKSPQEMEKVFQEVPQALTNTVKIAEEC
                     NLGLEFGKCHLPHFPLPQGYSDLDSFLTFLARKGLAERYQKVTPGMEERLDYELNVIK
                     QMGYAGYFLIVKDFIDYARSQKIPVGPGRGSAAGSLVSYSLGITNIDPLKYGLLFERF
                     LNPERVTLPDIDIDFSDRGRDDIIKYVINKYGKKNVAQIITFGSMAARAVVRDVGRVL
                     DIAYSEVDR"
BASE COUNT          839 a          544 c          714 g          794 t
ORIGIN      
        1 gaagacaaat gggggtttac gggaaaggag cgggaccaag accaaaggac aataaaggcg
       61 aaaataaaat gcttttcaac ttatgctcta ggccaggata ccatacctcc gtccataaga
      121 atcatccgtc ccaaagaaaa acagaagtta aaagttaagc gacctgaaat cgtaattaaa
      181 ataaaggacg acttctccgg aatcgaagac gacagagata tagaggccta cttagatgga
      241 gaatggctta ttccagaata tgacatagac aacaatatct tacgcaccaa accgcacttc
      301 gacttaggtt atggagagca cactttgact gtgcaagtta aagatagaat ggggaatacc
      361 agctcggcta aaagcagatt tgaggtaatt agtcaatgat acctcttaag gatgatatac
      421 cttcatatcg tcctccgatt gtgactattg gcttgatagt ggcaaactgc gttattttct
      481 ttcttcaact ctcttctgga agccaaggtt tcgagatgct gatttccaag tatggagcta
      541 ttccctatga gatcacccat cttggaacag tcaattcaca tccagctttt ccaatcggtt
      601 tgactctatt ttcttcaatg ttcatgcatg gcggtttttt tcatcttgtc ggtaatatgc
      661 tttacctttg gatatttgga gacaatgttg aggataggct gggtcacata aaatttttca
      721 tcttttacgt cacctctgga cttattgcca gtttttctta tattataact gcacccggct
      781 cgaacgttcc gatgataggt gcgtctggtg ccatctccgg agttctgggg gcttaccttt
      841 tgaagtttcc gagggcgcgc atatcgactc tgatcttttt tggtttcttt gttcgtatgg
      901 ttaagatacc cgcttttctg gttttaggtt tttggttcat cttacagctt ctgagtggct
      961 taccatcctt gggggcggag gcaagcggtg gagtggcctg gtttgcgcat atcggcggtt
     1021 ttctgggtgg aatgttactc ttgaaactat ttggaggtct ctgaaaaact gtatcggggt
     1081 agtcgggtct caagatgcag aattcgcaca aggtgcccga cctacgaaga atcaataact
     1141 tgcaaccacg tagacagggc tcccagccct gcaaaacgag ttttttagag ctctcattta
     1201 agggataaag atgaaataca aaacagaagc aaaaaagaaa gcccgctgcg ctaaatgcgg
     1261 aaagagattg aaaaagggtg gcttgaaata caacataaga gttgaagtgg cttcggattt
     1321 tgatggatac ttggaagatt actccaaaaa gcccctggat tatctggaga atagactaaa
     1381 gatactggaa gaggatttga aggatagaaa ggaattggaa ttggaagagg acgtttatat
     1441 tgagaggtcc ttcctggtgt gtggggagtg tcgggatcgg tttgtgaaaa tcttaaggga
     1501 gtttgaaggg gaagaatgaa acactccaat ttcgttcatt tacatattca tacccattat
     1561 agcttattgg acggtgcttg tagaatagat gacctggtca actcggcaag ggaatataag
     1621 atgcctgccc tggcaatcac tgaccacgga aatatgtttg gcatcgtgga gttttacaaa
     1681 aagatggtat ccgccgggat aaagccgata ataggttgtg aagtttatgt tgcccctgtc
     1741 agtcggcaca agaaggagcc ggttagaggg tttccggatg cagggtatca tctgatactt
     1801 ttggttaaag attcggttgg ttacaaaaat ctgattaagc tcgctaccat tggatactta
     1861 gagggtttct accacaagcc cagggtagat gaagaggtct tgaggaaata tagccaagga
     1921 ctggttgccc tttccagctg tctcaaagga gagattgcat gtttgattct ggctgagaaa
     1981 tacgatgaag tcaagaaagc agccaataag tatgtggaga tatttggtga aaacaatttc
     2041 tatttcgaac tgcaggacca cggcttggat gaagagaaga aagtcgccag agagctagtg
     2101 aaactatcta aagagatgtt gattcccttg gtcgctacga atgactgcca ttatctcaaa
     2161 caagaggatt atctggcaca tgatgctctg ctgtgcatcc aaactggcag aaaggtgacg
     2221 gatgaggata ggatgaggtt cgtctcagac caactttact tcaaatctcc gcaggagatg
     2281 gaaaaagtct tccaggaagt tccccaagcc ctaaccaaca ccgtaaaaat agccgaggag
     2341 tgcaatttag gtttggagtt cggcaaatgc catctaccgc attttcctct gcctcaagga
     2401 tattctgact tggattcttt tttgactttc ttggctagaa aggggttagc cgaaagatac
     2461 caaaaggtta cgccgggaat ggaagaaaga ctggattacg agctgaatgt gataaaacaa
     2521 atgggttatg ccgggtactt tttgattgtc aaggacttca tagactacgc caggtcacag
     2581 aaaataccag tcggtccggg cagaggctct gcagctggaa gtttggtttc ctactctttg
     2641 ggcataacca atattgaccc cttgaaatat ggacttctct ttgagagatt tctgaatccg
     2701 gaaagagtca cgttgccaga tattgacatt gatttttccg atagaggtag agacgatatc
     2761 ataaaatacg tcataaacaa atatggaaag aaaaatgttg cccagataat aacattcgga
     2821 agcatggctg cccgcgcagt ggtcagggat gttggcagag tattggatat cgcctattcg
     2881 gaagttgacc g
//
LOCUS       DASWLN010000165         3923 bp    DNA     linear   ENV 20-FEB-2024
DEFINITION  MAG TPA_asm: Candidatus Zixiibacteriota bacterium isolate
            SMAG_U2949 k141_844885, whole genome shotgun sequence.
ACCESSION   DASWLN010000165 DASWLN010000000
VERSION     DASWLN010000165.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37960594
            Sequence Read Archive: SRR10376091
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Candidatus Zixiibacteriota bacterium (soil metagenome)
  ORGANISM  Candidatus Zixiibacteriota bacterium
            Bacteria.
REFERENCE   1  (bases 1 to 3923)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 3923)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (26-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/12/2024 08:28:26
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 1,440
            CDSs (total)                      :: 1,416
            Genes (coding)                    :: 1,412
            CDSs (with protein)               :: 1,412
            Genes (RNA)                       :: 24
            rRNAs                             :: 1 (16S)
            partial rRNAs                     :: 1 (16S)
            tRNAs                             :: 21
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 4
            CDSs (without protein)            :: 4
            Pseudo Genes (ambiguous residues) :: 0 of 4
            Pseudo Genes (frameshifted)       :: 0 of 4
            Pseudo Genes (incomplete)         :: 3 of 4
            Pseudo Genes (internal stop)      :: 1 of 4
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..3923
                     /organism="Candidatus Zixiibacteriota bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_844885"
                     /isolate="SMAG_U2949"
                     /isolation_source="soil"
                     /db_xref="taxon:2053527"
                     /environmental_sample
                     /country="USA: Oregon"
                     /lat_lon="42.99 N 123.63 W"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            <1..226
                     /locus_tag="VGB16_05480"
     CDS             <1..226
                     /locus_tag="VGB16_05480"
                     /inference="COORDINATES: protein motif:HMM:NF026064.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=2
                     /transl_table=11
                     /product="ribosome biogenesis GTPase Der"
                     /protein_id="HEX9917171.1"
                     /translation="IGKEIKRRPPAAVGGKYIKIYYLTQTDIQPPTFVFFCNYPESLE
                     KAYLKFLSNCIREHFGFVGCSLRIKVRKRE"
     gene            227..877
                     /gene="plsY"
                     /locus_tag="VGB16_05485"
     CDS             227..877
                     /gene="plsY"
                     /locus_tag="VGB16_05485"
                     /EC_number="2.3.1.15"
                     /inference="COORDINATES: protein motif:HMM:TIGR00023.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0005886 - plasma membrane [Evidence IEA];
                     GO_function: GO:0008374 - O-acyltransferase activity
                     [Evidence IEA];
                     GO_process: GO:0006654 - phosphatidic acid biosynthetic
                     process [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="glycerol-3-phosphate 1-O-acyltransferase PlsY"
                     /protein_id="HEX9917172.1"
                     /translation="MPIGLLRFLAVIIFRYFLGSIPFGVIISRLFKGIDVREYGSKNI
                     GFTNVFRVVGILPAVIVLILDMGKGVLSILLVSQIVSSQVPLSLTLVKIGAGIFVILG
                     HIFPIFANFRGGKGVATAAGVLLALVPSEFILILVIFILIVSFTRYISLGSLISSLFL
                     PLILTFEKCYLKKAIPIELIIISLILCFLIFYTHRQNIKRLLRGTENKFGKKDKRI"
     gene            879..1886
                     /locus_tag="VGB16_05490"
     CDS             879..1886
                     /locus_tag="VGB16_05490"
                     /EC_number="1.1.1.94"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_013278019.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0009331 - glycerol-3-phosphate
                     dehydrogenase complex [Evidence IEA];
                     GO_function: GO:0047952 - glycerol-3-phosphate
                     dehydrogenase [NAD(P)+] activity [Evidence IEA];
                     GO_function: GO:0051287 - NAD binding [Evidence IEA];
                     GO_process: GO:0046168 - glycerol-3-phosphate catabolic
                     process [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="NAD(P)H-dependent glycerol-3-phosphate
                     dehydrogenase"
                     /protein_id="HEX9917173.1"
                     /translation="MSRVSILGAGGWGIALSVLLNHNQHQVSLWEFDKDDFQMLSKKR
                     ERKDKLPKVKIPQSVKITNELKEALSNQEYICLALPSHTVRGVAKKIAPLLYGNPIVI
                     NLAKGIENKTLCRMSEVLVGELPKKIRPNITTLSGPSHAEEVSRKVPTTVVVAGFNDK
                     TTEKVQKLFMNEFFRVYTHHDLIGVELGGSLKNIIAIASGICDGLGLGDNTKGALISR
                     GLAEMIRLGEKLGADAKTFAGLSGVGDLITTCFSKYSRNRYVGEEIGKGRKLKDVLSK
                     MSMVAEGVKTTRSAYRLSRKHKVEMPITHKVYQVLFEDKPPKLGLMELMTRQPKSEIW
                     N"
     gene            1992..2348
                     /locus_tag="VGB16_05495"
     CDS             1992..2348
                     /locus_tag="VGB16_05495"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_014857172.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003677 - DNA binding [Evidence IEA];
                     GO_function: GO:0003700 - DNA-binding transcription factor
                     activity [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="MerR family transcriptional regulator"
                     /protein_id="HEX9917174.1"
                     /translation="MAKFALKPGEKIYYSISEVSRMAGVKDFVLRFWEKEFPSLKPKK
                     NRAGNRSYQQKDIDMVNRIKHLLYEEGHTIEGARNRLKETKKDIKVKPDMKTAQLKNL
                     LFEVRKELKDMLKFFA"
     gene            2384..2458
                     /locus_tag="VGB16_05500"
     tRNA            2384..2458
                     /locus_tag="VGB16_05500"
                     /product="tRNA-Pro"
                     /inference="COORDINATES: profile:tRNAscan-SE:2.0.12"
                     /note="Derived by automated computational analysis using
                     gene prediction method: tRNAscan-SE."
                     /anticodon=(pos:2419..2421,aa:Pro,seq:ggg)
     gene            2808..3479
                     /locus_tag="VGB16_05505"
     CDS             2808..3479
                     /locus_tag="VGB16_05505"
                     /inference="COORDINATES: protein motif:HMM:NF012745.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016757 - glycosyltransferase activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="glycosyltransferase family 2 protein"
                     /protein_id="HEX9917175.1"
                     /translation="MTKTAIIIPAYNASTTLEKLISQLTNYVPPKDIIVIDDGSMDDT
                     YKIAKDKGAVIIQHEKNKGKGVALKTGFEYCLNHNYSGIITMDADLQHDPKFVQDFLK
                     MAKSSNYGIIIGTRQMNLKIMPLDRYLTNKICSIIISILSGKTIHDSQSGYRWISKEV
                     LEKVNLSSRRYDLESEILIKAGRGNFEIGEVEISTIYEKSKSFINPFVDTGRFIKVLI
                     KSIFW"
     gene            3597..>3923
                     /locus_tag="VGB16_05510"
     CDS             3597..>3923
                     /locus_tag="VGB16_05510"
                     /inference="COORDINATES: protein motif:HMM:NF014077.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016787 - hydrolase activity [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="5'/3'-nucleotidase SurE"
                     /protein_id="HEX9917176.1"
                     /translation="MRKKPIILLTNDDGIKAKGIVALESEMSRLGEVWVVAPDKEQSA
                     TSHSITLTCPLRTRKLGSRVFSIDGTPTDAVMVAVYALMKKRPSLIISGINHGSNLGD
                     DVTYSGT"
BASE COUNT         1222 a          758 c          889 g         1054 t
ORIGIN      
        1 aatcggaaag gagataaaga gaaggccacc ggctgcagtt ggaggaaaat acataaaaat
       61 atattatctt acccagactg atattcagcc accaacattt gttttcttct gtaattatcc
      121 ggagtcatta gaaaaggcgt atttgaaatt tctttccaat tgcattcgag aacattttgg
      181 atttgtggga tgttctttga ggattaaagt cagaaagaga gaataaatgc caattggctt
      241 gttaagattt ctcgctgtaa tcattttccg ctactttcta ggttccattc cttttggagt
      301 aatcatctcc agacttttca aggggatcga tgttcgcgaa tatggctcga aaaacatagg
      361 attcaccaat gttttccggg tagtaggaat cttgccagca gttattgtct tgattttgga
      421 tatggggaaa ggagtgctta gtattcttct ggtaagtcaa atagttagta gccaagttcc
      481 tttaagccta actttagtta aaattggggc ggggatattt gtaatcttag gccatatatt
      541 tcccatcttc gcgaacttcc gaggaggcaa gggtgtagca acagcagctg gtgttctttt
      601 ggcacttgtc ccttcggagt tcatacttat cctcgtcatc ttcatcttga tagtaagttt
      661 cacccgctat atttctctcg gctctttgat ttcatctttg tttttaccct tgattctaac
      721 tttcgaaaaa tgttacttga aaaaagcgat acctattgag ttaattataa tttctttaat
      781 attgtgcttc ttgattttct atacccaccg gcagaacata aaaaggctcc ttcgcggaac
      841 ggaaaacaaa ttcggcaaga aagacaaaag gatttgatat gtccagagtt tcgattttag
      901 gtgcaggagg ttggggcata gccctctcgg ttttactaaa tcacaaccaa caccaagtca
      961 gtttatggga attcgacaaa gatgatttcc agatgttgtc caaaaaacgg gaacgtaaag
     1021 ataaactccc caaggtgaaa attccccagt cagtcaagat aacaaatgag ctgaaagaag
     1081 cattaagcaa tcaggaatat atctgccttg ctttgccctc tcacacagta agaggggttg
     1141 cgaaaaaaat cgctccgctc ttgtacggaa acccgattgt gataaaccta gccaagggga
     1201 tagaaaacaa aaccctttgc aggatgtcag aggttttggt tggtgaattg cccaagaaaa
     1261 tccgtccgaa tatcactacc ctttccggac cgagccatgc ggaagaagta agccgaaaag
     1321 ttcctacaac tgtggtggta gccggattta atgataaaac aacagagaag gtgcagaagc
     1381 tttttatgaa cgagttcttt cgggtttata ctcatcacga tttaattggt gtggaattgg
     1441 gtggctcctt gaaaaatata attgccatcg cctccggcat ttgtgatggt ctgggtctgg
     1501 gagataacac caaaggggct ttaatttcac ggggcttagc agaaatgatt cgcttgggag
     1561 agaaattggg agcagatgcc aagacctttg ccgggctttc cggagtggga gatttaatta
     1621 ccacttgctt cagcaaatac tccagaaatc gctatgtggg agaagagata ggaaaaggaa
     1681 ggaagttgaa agatgtatta tccaagatga gtatggtggc agaaggagtg aaaaccactc
     1741 gctctgcgta ccggctttca cggaaacata aagtggaaat gcccataacc cacaaggtct
     1801 atcaggttct ttttgaggat aagcctccaa agttgggttt aatggagctg atgacccgac
     1861 aaccaaaaag tgaaatttgg aattgaccct gaaaaataaa attttggggt tgacataaaa
     1921 aatgtattag ttattttata gtgttagttt aagtgggggc ttctaacttc cctttattca
     1981 atcaaataag tatggcaaaa tttgccttaa agcccgggga gaagatttac tattcaatta
     2041 gcgaagtcag ccgaatggcc ggggtcaagg attttgttct gcgcttctgg gaaaaggaat
     2101 tcccctcact caagcccaag aagaatcggg cgggaaatcg ctcctatcag caaaaagata
     2161 tcgatatggt gaatcgcatc aagcatcttc tgtatgagga aggtcatacc atcgagggag
     2221 cgagaaacag actcaaagag accaaaaagg acatcaaagt caaacctgat atgaaaacag
     2281 ctcagcttaa gaatttgctc tttgaggtga ggaaagaatt aaaagatatg ctcaagtttt
     2341 tcgcttgatt tccggctgac tttgatttta tttgaacatt cctcggggcg tagcgcagcc
     2401 tggttagcgc actcgcttgg ggtgcgagtg gtcggccgtt caaatcggct cgccccgatt
     2461 agaaatttca gggcagagga aatctgctct tttttgtggt agaggatttg attgtcgtag
     2521 ggacaggtcg cgacctgtcc ctacaaaaaa tacagaatcg taggcgaccc atctgggtcg
     2581 cctgcaaaaa agcagggcgg gataaacatg ttctgtccct acaaaataag aatatgagat
     2641 tgcttcgtcg cttacgcttc tcgcaatgac gttataatga tgtcattaca aaaatgatag
     2701 atagattctt ggagcataaa gcttcagctt tatattatca aacctgaagg tttacactcc
     2761 aaaataggcg acattggcca atgtcgcctg aggctcggaa ttttgctatg accaaaacag
     2821 ccataatcat ccccgcttac aacgcctcta ccactttgga aaaattgatt tcgcagctta
     2881 caaattatgt acccccaaaa gatataatag taatcgatga cgggtcaatg gatgatactt
     2941 acaaaatcgc aaaagacaaa ggagctgtca ttattcaaca cgaaaagaac aaaggaaaag
     3001 gagtagcttt aaaaacaggg tttgaatatt gtctaaatca caattactcc ggcataatca
     3061 caatggatgc ggatttgcag cacgacccta agtttgtgca ggatttcctg aaaatggcaa
     3121 aaagttcaaa ctatggcatc ataattggca cacgccagat gaatctaaag attatgccac
     3181 ttgatagata cttgactaat aagatatgtt ctattataat atcaattctt tcgggcaaga
     3241 ccattcatga tagccaatcc ggttatcgct ggatttcaaa ggaagtttta gagaaagtaa
     3301 acttgagttc cagaagatat gacctggaat ctgaaatctt aattaaggcg ggtcggggga
     3361 attttgaaat cggtgaagtt gaaatatcga cgatatacga aaaaagcaaa agctttatta
     3421 acccattcgt agataccggc agattcataa aagttctaat aaaaagcata ttctggtgaa
     3481 gcctcccgta acaaatcaca gggcttcaca aatataaagg gccctcggct tccctcggct
     3541 tcgccgagag gcgagcgccg agaggcgagc gaggctttct ggcagaggcg agtaaaatga
     3601 gaaaaaaacc aatcatactt ttaactaacg atgacggaat taaagcaaag ggaatagttg
     3661 ccttagaaag cgagatgtcc agactcggag aggtgtgggt agttgctccg gacaaggagc
     3721 aaagcgccac cagccattcc ataaccctaa cctgtccctt gagaacaaga aaacttgggt
     3781 caagagtttt ctccatagac ggcacaccaa cagatgcagt gatggtagcg gtttatgccc
     3841 ttatgaagaa aagaccgagc ttgattatct ccggcataaa tcacggctca aatttgggag
     3901 acgatgtaac ctattccgga acc
//
LOCUS       DASWLN010000166         2115 bp    DNA     linear   ENV 20-FEB-2024
DEFINITION  MAG TPA_asm: Candidatus Zixiibacteriota bacterium isolate
            SMAG_U2949 k141_220856, whole genome shotgun sequence.
ACCESSION   DASWLN010000166 DASWLN010000000
VERSION     DASWLN010000166.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37960594
            Sequence Read Archive: SRR10376091
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Candidatus Zixiibacteriota bacterium (soil metagenome)
  ORGANISM  Candidatus Zixiibacteriota bacterium
            Bacteria.
REFERENCE   1  (bases 1 to 2115)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 2115)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (26-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/12/2024 08:28:26
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 1,440
            CDSs (total)                      :: 1,416
            Genes (coding)                    :: 1,412
            CDSs (with protein)               :: 1,412
            Genes (RNA)                       :: 24
            rRNAs                             :: 1 (16S)
            partial rRNAs                     :: 1 (16S)
            tRNAs                             :: 21
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 4
            CDSs (without protein)            :: 4
            Pseudo Genes (ambiguous residues) :: 0 of 4
            Pseudo Genes (frameshifted)       :: 0 of 4
            Pseudo Genes (incomplete)         :: 3 of 4
            Pseudo Genes (internal stop)      :: 1 of 4
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2115
                     /organism="Candidatus Zixiibacteriota bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_220856"
                     /isolate="SMAG_U2949"
                     /isolation_source="soil"
                     /db_xref="taxon:2053527"
                     /environmental_sample
                     /country="USA: Oregon"
                     /lat_lon="42.99 N 123.63 W"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            complement(<1..206)
                     /locus_tag="VGB16_05515"
     CDS             complement(<1..206)
                     /locus_tag="VGB16_05515"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEX9917177.1"
                     /translation="MNWGNLCLVLSLIGASFSALFFFLEARRNREISSFGKKAYYFFV
                     FFTTLATGYLFFLFLTHRFEFEYI"
     gene            279..455
                     /locus_tag="VGB16_05520"
     CDS             279..455
                     /locus_tag="VGB16_05520"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEX9917178.1"
                     /translation="MPDLSSHFRVAIVLILHILASWLIVKTGSWLENKVLTKKSEFYI
                     IDLITKDTIATNRK"
     gene            467..556
                     /locus_tag="VGB16_05525"
                     /pseudo
     CDS             467..556
                     /locus_tag="VGB16_05525"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_014258485.1"
                     /note="catalyzes the transfer of electrons from NADH to
                     ubiquinone; incomplete; partial in the middle of a contig;
                     missing N-terminus; Derived by automated computational
                     analysis using gene prediction method: Protein Homology."
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="NAD(P)H:quinone oxidoreductase"
     gene            616..1173
                     /locus_tag="VGB16_05530"
     CDS             616..1173
                     /locus_tag="VGB16_05530"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_012174188.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hemerythrin domain-containing protein"
                     /protein_id="HEX9917179.1"
                     /translation="MQNNKNFNMMPIAPLMIEHRLIEKMIKLMEKELHKINQGNEANP
                     VFIEKAVDFFGTYADRCHHGKEEDILFRELAKKKLSSEHRKMMDELINEHIFGRKIVG
                     RLANAKDKYTNTDYDTTKEIVECLKTLVGFYPSHIKKEDKQFFIPCMEYFSQEEKDEM
                     LDECWEFDRNIIHEKYQNIVKQLGA"
     gene            1215..>2115
                     /locus_tag="VGB16_05535"
     CDS             1215..>2115
                     /locus_tag="VGB16_05535"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_018963673.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016903 - oxidoreductase activity, acting
                     on the aldehyde or oxo group of donors [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="2-oxoacid:acceptor oxidoreductase family
                     protein"
                     /protein_id="HEX9917180.1"
                     /translation="MSKDVFTFLVGGKAGEGVKKAGSVAAQLFADMGRQVFQMDDYQS
                     LIKGGHNFSIVSTAKRKITSHYAKADLIVALDKRSYDLHKTDLTDNGIMVYNSDSVEQ
                     VGGVGVPLTIEANKYPNPNLRIGVGAVASLAAAVGIDKDDLKKLIENEYPRDIENNIA
                     YAIAIYDMLYPKIGDKFQLEKGDKKRPIVTGNEAIGLGATAGGLDIFFAYPMTPSSTL
                     LHFLAAHDKELGVTVVHPENEIGVINMALGSIFSGARAMVGSSGGGFALMEEAFSLAG
                     MLEAPLLCYLGSRPGPSTGVPTYS"
BASE COUNT          712 a          379 c          470 g          554 t
ORIGIN      
        1 tatatatatt caaattcaaa gcggtgggtt aagaaaagaa aaaacagata gcccgtggca
       61 agcgttgtaa aaaagacgaa aaagtagtag gccttttttc cgaaagagga gatttccctg
      121 ttccttctgg cttccaaaaa gaaaaacaaa gctgaaaatg aagccccaat cagcgaaaga
      181 accagacaaa gatttcccca attcatttca agctctcatt ttgatttgca aataatagat
      241 gtaaaacaaa acttgtttga aaaccaatat cattcagctt gccagacttg tcaagccatt
      301 tcagagtagc aatcgtatta atcttacata tcttagcatc ttggctgatt gtcaagacag
      361 ggagttggtt ggaaaataaa gtcttgacca agaagagtga gttttatatc atagatttga
      421 taactaaaga tactatagct acaaaccgga aatgatatag attagaggag agcgtatgcc
      481 gagtgaaaat gagcttgccg ccacacgctt tcaaggaaaa catgtggcta tgattgcctc
      541 caaacttgca aattaatcat atccttttgg gcaaattcat tcttaggtta tagtttaatt
      601 tttggcgagg gaaaaatgca aaataataaa aactttaata tgatgccgat tgctcctttg
      661 atgatagagc acagacttat tgagaaaatg atcaaattaa tggaaaagga attgcataag
      721 ataaatcaag gaaacgaagc caatcccgtt ttcattgaaa aagcagtcga tttctttgga
      781 acctatgctg atcgctgcca tcatggcaaa gaagaggata ttctcttcag agaacttgcc
      841 aagaaaaaac tttcctcaga acacagaaaa atgatggatg agcttatcaa tgaacatatc
      901 tttggaagaa agattgtggg cagattggca aatgcaaaag ataaatatac aaataccgat
      961 tacgatacca caaaagaaat agtcgaatgc ctaaagaccc tagttggatt ttatccttcg
     1021 catattaaaa aagaagataa acagtttttc attccttgta tggaatactt tagtcaggaa
     1081 gaaaaggacg aaatgcttga tgagtgctgg gaattcgata ggaatattat tcacgaaaag
     1141 taccagaata tagtaaaaca gcttggggct taaaactact ttggcttata aataacaaat
     1201 caggagaaac tcaaatgagc aaagatgttt tcacttttct tgtcgggggc aaggctggcg
     1261 aaggcgtaaa aaaagcaggc tcggtcgcgg cccagttatt tgcggatatg ggtcgtcagg
     1321 tttttcaaat ggacgattac cagagcttaa tcaaaggagg ccataacttc tccattgtca
     1381 gcacagccaa aagaaaaatc accagccact atgccaaagc ggatttgata gtggcgctgg
     1441 ataagaggag ttatgatttg cacaaaacgg atttgactga taatggcatt atggtttaca
     1501 actcggatag cgtggaacaa gtcggaggtg tgggcgtgcc attgacgatt gaggcaaaca
     1561 aatatcccaa tcctaactta aggattggag ttggggcagt agcatcgctt gccgctgctg
     1621 ttggaattga taaagatgat ttgaaaaagc ttattgaaaa cgaatatccc cgggacatcg
     1681 aaaataacat tgcttatgcc atagccatat acgatatgct atatccgaaa ataggagaca
     1741 aattccaact ggaaaaaggc gacaaaaaaa gaccgattgt gaccggcaat gaggcaatag
     1801 gattgggagc cacagccggc gggttagaca tcttttttgc ctatcccatg acgccttcct
     1861 ccactctttt gcatttcctt gcggctcacg ataaagagct tggagtaacg gtggtgcatc
     1921 ctgaaaatga aattggggtt ataaatatgg cattaggttc aattttttcc ggagcaaggg
     1981 caatggttgg cagtagtggc gggggttttg ctctgatgga agaagccttc tctctggcgg
     2041 gaatgttgga agcgcctctt ttatgctatt tagggtccag acccggacca tctaccggcg
     2101 tgcccactta ctcct
//
LOCUS       DASWLN010000167         4568 bp    DNA     linear   ENV 20-FEB-2024
DEFINITION  MAG TPA_asm: Candidatus Zixiibacteriota bacterium isolate
            SMAG_U2949 k141_1228565, whole genome shotgun sequence.
ACCESSION   DASWLN010000167 DASWLN010000000
VERSION     DASWLN010000167.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37960594
            Sequence Read Archive: SRR10376091
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Candidatus Zixiibacteriota bacterium (soil metagenome)
  ORGANISM  Candidatus Zixiibacteriota bacterium
            Bacteria.
REFERENCE   1  (bases 1 to 4568)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 4568)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (26-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/12/2024 08:28:26
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 1,440
            CDSs (total)                      :: 1,416
            Genes (coding)                    :: 1,412
            CDSs (with protein)               :: 1,412
            Genes (RNA)                       :: 24
            rRNAs                             :: 1 (16S)
            partial rRNAs                     :: 1 (16S)
            tRNAs                             :: 21
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 4
            CDSs (without protein)            :: 4
            Pseudo Genes (ambiguous residues) :: 0 of 4
            Pseudo Genes (frameshifted)       :: 0 of 4
            Pseudo Genes (incomplete)         :: 3 of 4
            Pseudo Genes (internal stop)      :: 1 of 4
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..4568
                     /organism="Candidatus Zixiibacteriota bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_1228565"
                     /isolate="SMAG_U2949"
                     /isolation_source="soil"
                     /db_xref="taxon:2053527"
                     /environmental_sample
                     /country="USA: Oregon"
                     /lat_lon="42.99 N 123.63 W"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            <1..1479
                     /gene="ptsP"
                     /locus_tag="VGB16_05540"
     CDS             <1..1479
                     /gene="ptsP"
                     /locus_tag="VGB16_05540"
                     /EC_number="2.7.3.9"
                     /inference="COORDINATES: protein motif:HMM:TIGR01417.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008965 - phosphoenolpyruvate-protein
                     phosphotransferase activity [Evidence IEA];
                     GO_process: GO:0006808 - regulation of nitrogen
                     utilization [Evidence IEA];
                     GO_process: GO:0009401 - phosphoenolpyruvate-dependent
                     sugar phosphotransferase system [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="phosphoenolpyruvate--protein phosphotransferase"
                     /protein_id="HEX9917181.1"
                     /translation="NVVSKIEETRRNAEYVYRQVIQKMIQELSASKDEYIRERILDIN
                     EVSHRLLYNLLGIKRKTMETVDRQVILIAKTLSPGDVVQMRREFVLGFATDTGGGTSH
                     VAILAKSLGIPAVVGLKDCFTRVENGVQVVLDGNRGEIMLHPDQNTIRRYAEEKENLL
                     TQSKRFLESKSLPAETLDGRRIELSANIELASDVEHALEYGAEGVGLFRTEYLYLTRS
                     DLPTEEEQYKAYLKVAQKMAPKPAILRTFDLGGDKFSKGLGDSYEANPFLGWRAIRAC
                     LDLPELFKVQLRAMLRASSLGNLKIMFPMISSLKELLRAKEILEEVKLELRSQGEPID
                     EKLQVGIMVEIPSAALLADSLAKECDFFSIGTNDLIQYTLAVDRGNEKISYLYQDFHP
                     SVLKLIKEVIEAGHRNKIWVGLCGEMAGEPKAIPLLVGMGINELSTSPIAIPQVKSII
                     RSIKFKEAKKLVDKVLTLSQTEEIEKVLEDYCRQVSLQCENK"
     gene            1493..2554
                     /locus_tag="VGB16_05545"
     CDS             1493..2554
                     /locus_tag="VGB16_05545"
                     /EC_number="5.3.1.9"
                     /inference="COORDINATES: protein motif:HMM:NF006426.0"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="bifunctional phosphoglucose/phosphomannose
                     isomerase"
                     /protein_id="HEX9917182.1"
                     /translation="MLDDSNALRKSDPSGMYNLIYNFPSQFKNASTLGKSLLLPDWRA
                     RDIKNIVLVGMGGSAIGGDLVRSYLAGELKVPFLINRSYTLPNLVGPASLVFLSSYSG
                     NTEETLSAYDQAKERKAKIVCISSGGKLLEQAVQDGFFSIKIPEGYPPRAALGYSFVL
                     PLMVLAFLKLVPDKEKEMEETIKSLTNGVNVYKMERKTSENPAKNLALRLHKKLPIIY
                     AAENHLDAVATRWKGQVCENAKMLAFSNLFPEFNHNELVGWKVIDGYKDKLVVIVLKD
                     RDDHIRIKRRMEIVKGIIEKMGVEVIEKESAGEGLLSRIFSLIQLGDFTSFYLAILNQ
                     IDPFPVDIINFLKDELAKE"
     gene            2566..3726
                     /locus_tag="VGB16_05550"
     CDS             2566..3726
                     /locus_tag="VGB16_05550"
                     /inference="COORDINATES: protein motif:HMM:NF042826.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005515 - protein binding [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="fibronectin type III domain-containing protein"
                     /protein_id="HEX9917183.1"
                     /translation="MNTFSKKALFLFSFFLFVFSFSRADELRPPSDLKAFDTPDDGGG
                     GVTFSWKLSPHDREPLGDIDGYEIYRKAKEDSTYRQIDFLPDNQTTYNDYDLKDKTQY
                     SYAIRARKGEEFSGYIYSNYVSPKPNWFRFGRTNVLLMLLVFSLAVLLFIRSAKRGKP
                     LFVRKINGLDAIEEGVGRATEMGKSVLFVPGIGDVDDIQTIAGLSILSRVAHITAEYE
                     TRLICPVLYPLPFAAAQEMVKSAYIDEGKAEDFKPDTVRYVAGEQFAYVAAVSGIMFR
                     EKPAANLFMGAFYAESLLLAESGQSTGAIQVAGTANPEQLPFFIAACDYTLMGEELFA
                     ASAYLSKEPLMLGSLKGQDWMKIILIAIVIVGVVLETLHLGGGFFTKLFVEH"
     gene            3728..4348
                     /locus_tag="VGB16_05555"
     CDS             3728..4348
                     /locus_tag="VGB16_05555"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEX9917184.1"
                     /translation="MRTGLPVIIAFLAGMTMVVAFFIPNKSLEGFSQGFLVWMTIVGG
                     FTLLLGIVNILRNNLKIISQRGEGWGYKVILVLMLLAMGVSSFMWGTSRDTPYDWLFQ
                     NIQKPMQATMFSVLAFFVASAAYRAFRARSKEATLLLLTSVVVMLGRIPLGQFIWGKL
                     PLAANWIMGYPSLAVQRGIIIGAALGAASMSLRIILGIERAYMGKG"
     gene            4387..>4568
                     /locus_tag="VGB16_05560"
     CDS             4387..>4568
                     /locus_tag="VGB16_05560"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEX9917185.1"
                     /translation="MKTENSTNIFFKLTNLDRRYIYLLIGIAVFIPLIFPLRLPLGVT
                     PESEALFDKVESLKEGS"
BASE COUNT         1284 a          877 c         1161 g         1246 t
ORIGIN      
        1 aatgtggtaa gtaagattga ggagaccaga agaaatgctg agtatgttta tagacaagtt
       61 attcaaaaga tgattcagga gctttccgcc tccaaggatg aatatatccg cgagaggatt
      121 ttggatatca atgaggtaag ccatcggctt ctgtataatc tcctggggat aaaacggaaa
      181 accatggaga ctgttgaccg ccaggtgatt ctaattgcca agaccctttc tcctggggat
      241 gtggtgcaga tgagaaggga attcgtcttg gggtttgcta ctgatactgg aggagggacc
      301 tcgcatgttg ccatcctggc aaaatcctta ggcattccgg cagtagtcgg gctgaaggat
      361 tgcttcactc gagtggagaa tggtgtacag gtggtgttgg atgggaatag gggggaaata
      421 atgttgcatc cagaccagaa taccatccgg cgttatgcag aggaaaagga aaatctcctt
      481 actcaaagca agaggttttt agaatcgaaa tccctaccag cggagacctt ggatggcaga
      541 aggatagagc tttctgccaa tatcgagctt gcttcggatg tggaacatgc gttggaatat
      601 ggagcggaag gggttgggct gtttcgcacc gaatacctct acttgactcg ctctgacctt
      661 cccactgagg aggagcagta caaagcatat ctaaaagtag cacaaaagat ggcacccaag
      721 ccagctatcc tgagaacttt tgatttggga ggagataagt tctcgaaggg tttgggcgat
      781 tcctacgaag ccaacccatt tttaggctgg agggcaataa gagcttgcct ggatttgccc
      841 gaattgttta aggttcaact gagagctatg ctaagggcta gctctttggg caatctcaag
      901 attatgtttc ccatgatctc tagcttgaag gaacttttga gggcaaagga gatcttggaa
      961 gaagtgaaac tcgagcttag aagccagggt gagccgatcg atgagaaact acaagtgggg
     1021 ataatggtgg agataccatc cgccgccctc ttagctgatt ctttagctaa agaatgcgat
     1081 ttcttcagca tcgggacaaa tgacctgatt caatataccc tggccgtgga ccggggaaac
     1141 gagaagatat cttatcttta tcaggatttt cacccttcag tattaaaatt gattaaagag
     1201 gtaatagagg caggtcatcg gaacaagatt tgggtggggt tatgcggcga gatggcaggt
     1261 gagcccaaag ccataccgct tctggtaggg atgggtatca atgaactttc cactagtccc
     1321 atagccatac cccaagtgaa gagcattata cgttcgataa agttcaaaga agccaagaaa
     1381 ctagttgata aggtattgac cctttcgcaa accgaggaga tagaaaaagt attggaggat
     1441 tattgtcgtc aagtttcact tcaatgcgag aataaataat caggaggaaa tcttgttgga
     1501 tgattcaaac gcattaagaa aatctgatcc atctggaatg tataatttga tttataattt
     1561 tccctctcag ttcaagaatg cctcaacttt gggcaaaagc ttgctcttgc cggactggag
     1621 agccagagac attaagaata ttgttttggt agggatgggt ggctctgcca ttggtgggga
     1681 tttggtccgt tcctatttag ccggtgagct taaagtcccc ttcttgataa atcgcagcta
     1741 cactttaccg aatcttgtgg gacctgccag cttggttttt ctttccagtt actccggcaa
     1801 tactgaagag actctttccg cctatgacca agccaaggaa agaaaggcca aaatcgtctg
     1861 catctcctca ggtggaaaac ttttggagca ggcagtccag gatggattct tctccatcaa
     1921 gattcccgaa ggttacccgc ccagagcggc tttaggatat tcttttgttt tgcccttgat
     1981 ggttttagct tttttgaagt tagtgccgga caaagaaaag gaaatggagg agaccattaa
     2041 gtcgcttacc aatggagtta acgtctacaa gatggaaaga aaaacttcgg aaaatccagc
     2101 caagaattta gctttgagat tgcacaaaaa actccccata atctatgcag cggagaatca
     2161 tctggatgca gttgccacca gatggaaagg gcaggtctgc gagaatgcca agatgctggc
     2221 cttctctaat ctttttccag aattcaatca caatgagttg gttggatgga aggtcattga
     2281 cgggtacaaa gacaagttag tggtaatagt gttaaaagac cgagatgacc acatacgcat
     2341 aaaaagaaga atggagatag taaaggggat aatagagaag atgggagtgg aggtgattga
     2401 aaaggaaagt gcaggcgagg gactgctctc ccgtattttt tctttaatcc aattgggaga
     2461 tttcaccagt ttctatctgg caattttaaa tcagattgac ccatttccag tggacataat
     2521 taattttctc aaagatgaat tagctaaaga atagcaagga ataagttgaa cactttttca
     2581 aaaaaggcac tctttctatt ttccttcttc ctttttgttt tttcctttag ccgggcagat
     2641 gagcttagac ccccaagcga tttaaaagca ttcgatactc cagatgatgg gggaggtggg
     2701 gtaactttta gctggaagct ctccccgcat gacagagagc ccttggggga tattgatgga
     2761 tatgaaattt acagaaaagc caaggaagat tccacctacc gacaaataga tttcttacca
     2821 gataatcaga ccacctataa cgattacgac ctgaaagaca aaactcagta ctcctatgcc
     2881 atccgggcaa gaaaaggtga agagttttca ggttacatct attccaacta cgtttctccc
     2941 aaaccaaatt ggtttcgttt tggaaggact aatgtgcttt tgatgctttt ggttttctct
     3001 ttggctgtgc ttcttttcat tcgcagcgcc aaaagaggaa agccgctttt cgtgcgcaag
     3061 ataaatggtc tggatgcgat tgaagagggt gtgggacggg caacagagat gggcaagtca
     3121 gttctctttg tgccggggat tggagatgtg gatgatattc agaccattgc cggcttgtcc
     3181 attttaagcc gggtagccca tatcacagct gaatacgaga ccaggctcat ctgcccagtt
     3241 ctctatcctt taccttttgc cgccgcacag gagatggtga aaagcgccta catagacgaa
     3301 ggaaaagcag aggatttcaa gccggacaca gtgagatatg ttgccggaga gcaatttgct
     3361 tacgtcgcgg cagtaagtgg aattatgttc agagaaaaac ccgccgccaa tctgtttatg
     3421 ggcgcatttt atgctgaatc gcttctcttg gcggagagcg gacaatccac cggagccatt
     3481 caggtggcgg gaaccgccaa tccggaacaa cttccctttt tcattgcagc ttgcgactac
     3541 actttaatgg gagaggagct ctttgcagcc agcgcctatc tttccaagga gcccttgatg
     3601 ttgggcagtt tgaaaggtca ggattggatg aagataattt tgatagccat agtaatcgta
     3661 ggtgtggttt tggaaacatt gcatttgggc ggagggtttt tcaccaaact atttgtggag
     3721 cattaaaatg cgaaccggac ttccagtgat aatcgctttc ttggctggta tgacaatggt
     3781 ggtagctttt ttcattccta ataagagttt ggagggtttc agtcaaggat ttttggtgtg
     3841 gatgacaatt gtgggtggat tcactttgct tttaggcata gttaatatct taagaaacaa
     3901 tctgaaaatc ataagccaaa gaggagaagg gtgggggtac aaggttatcc ttgttctcat
     3961 gcttctggct atgggggttt ccagttttat gtgggggaca tccagggata caccctacga
     4021 ttggctattt cagaatattc aaaagccgat gcaggcgact atgttttcgg tcttggcatt
     4081 tttcgttgcc tccgcagctt atcgcgcttt tcgcgccagg agcaaggagg caactctttt
     4141 actcctgacc tctgtggtgg tgatgttggg aaggatacct ttgggccaat tcatctgggg
     4201 gaaactacct ttggctgcca attggataat gggctatccc agcttagcgg tacaaagagg
     4261 gataatcatc ggtgcggctt tgggggcagc ctctatgtct ttgagaataa ttctgggcat
     4321 agaaagagca tatatgggaa aaggatagct aatcaagaat taaaaaagaa aaatgaaaaa
     4381 ttaaaaatga agacagaaaa ttccacaaat atctttttca agctgacgaa ccttgacaga
     4441 agatacattt atctattgat cgggattgcg gtatttatac ctttgatttt ccctttgcgc
     4501 ctgcccttgg gagtaactcc tgaatcggaa gctctttttg ataaggtgga aagcttaaag
     4561 gaaggttc
//
LOCUS       DASWLN010000168       138818 bp    DNA     linear   ENV 20-FEB-2024
DEFINITION  MAG TPA_asm: Candidatus Zixiibacteriota bacterium isolate
            SMAG_U2949 k141_222584, whole genome shotgun sequence.
ACCESSION   DASWLN010000168 DASWLN010000000
VERSION     DASWLN010000168.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37960594
            Sequence Read Archive: SRR10376091
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Candidatus Zixiibacteriota bacterium (soil metagenome)
  ORGANISM  Candidatus Zixiibacteriota bacterium
            Bacteria.
REFERENCE   1  (bases 1 to 138818)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 138818)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (26-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/12/2024 08:28:26
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 1,440
            CDSs (total)                      :: 1,416
            Genes (coding)                    :: 1,412
            CDSs (with protein)               :: 1,412
            Genes (RNA)                       :: 24
            rRNAs                             :: 1 (16S)
            partial rRNAs                     :: 1 (16S)
            tRNAs                             :: 21
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 4
            CDSs (without protein)            :: 4
            Pseudo Genes (ambiguous residues) :: 0 of 4
            Pseudo Genes (frameshifted)       :: 0 of 4
            Pseudo Genes (incomplete)         :: 3 of 4
            Pseudo Genes (internal stop)      :: 1 of 4
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..138818
                     /organism="Candidatus Zixiibacteriota bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_222584"
                     /isolate="SMAG_U2949"
                     /isolation_source="soil"
                     /db_xref="taxon:2053527"
                     /environmental_sample
                     /country="USA: Oregon"
                     /lat_lon="42.99 N 123.63 W"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            complement(170..1282)
                     /gene="dnaN"
                     /locus_tag="VGB16_05565"
     CDS             complement(170..1282)
                     /gene="dnaN"
                     /locus_tag="VGB16_05565"
                     /EC_number="2.7.7.7"
                     /inference="COORDINATES: protein motif:HMM:TIGR00663.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0009360 - DNA polymerase III complex
                     [Evidence IEA];
                     GO_function: GO:0003887 - DNA-directed DNA polymerase
                     activity [Evidence IEA];
                     GO_process: GO:0006260 - DNA replication [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="DNA polymerase III subunit beta"
                     /protein_id="HEX9917186.1"
                     /translation="MKFSILKSKLFDSIQKTLNAVPGKTPLPILSNFLLEFEETKLKI
                     SATDLDVSITTSIPIKSTKKGSLVIPARTFTDIIREAPETEIEINITDERLEMRIDKG
                     SYKISGSSSEEYPKIPSIQVAKEINIPGETLVRMIKKVGFAASMDETRPALSGILWQT
                     SGEVMCMAATDGHRLAKVALQNTKLKGLHKDIIIPPKALNLLVKLYQGDEELGVIFGE
                     NNIIFNLGDVVLSSRLIEGPYPDYEQVIPKDNDKKLIVNKELLASLVRRVSVLSNTLT
                     HQIKFSLKKDSLELSATNAEVGGEAIETLPCNYKNEIMEIGYNANYILDILKQVDGDE
                     VVFELATPVSAGMVHSASKEKGEEYLCLIMPLRLNE"
     gene            complement(1499..2845)
                     /gene="dnaA"
                     /locus_tag="VGB16_05570"
     CDS             complement(1499..2845)
                     /gene="dnaA"
                     /locus_tag="VGB16_05570"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_014096603.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003677 - DNA binding [Evidence IEA];
                     GO_function: GO:0003688 - DNA replication origin binding
                     [Evidence IEA];
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_process: GO:0006270 - DNA replication initiation
                     [Evidence IEA];
                     GO_process: GO:0006275 - regulation of DNA replication
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="chromosomal replication initiator protein DnaA"
                     /protein_id="HEX9917187.1"
                     /translation="MKNGPQIWEDCLGFLQHKVKKQSFFTWLRPTKGLDFSDDELRIE
                     VPNRFVAEWIEEHYPSLIREAIKSVTQKELNPIFCIRTGAQRSYEPSQNNKASLDQKT
                     RYHPAISPQLNSRYTFDTFVVGESNRFAHAAAMAVAEAPGKTKFNPLYIYGGVGLGKT
                     HLAQAIGHLALEGYKNIKVLYVTSETFTNDFINSISTSTMQEFVNRYRSVDVLLLDDI
                     QFFAGKESTQVQFFHTFNALYQSGKQIVLTSDRSPKEIKGLEERLLSRFQWGLVTDIQ
                     PPDLETRIAILNKKIESDSGSVPPEVVTFIADKITSNIRELEGSLIRLLAYASLNSKE
                     ITVDLAQEVLKDSIKNGVKLITIEQIQKKVAQNYNIPEDVLCSKKKTQEVVTARQVAM
                     YLARNLTNSSLKTIGLKFGGRDHSTVIHSCTQITENLKTNPEFKRKIDELISSLYV"
     gene            3325..3471
                     /gene="rpmH"
                     /locus_tag="VGB16_05575"
     CDS             3325..3471
                     /gene="rpmH"
                     /locus_tag="VGB16_05575"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_014855813.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0000315 - organellar large ribosomal
                     subunit [Evidence IEA];
                     GO_component: GO:0022625 - cytosolic large ribosomal
                     subunit [Evidence IEA];
                     GO_function: GO:0003735 - structural constituent of
                     ribosome [Evidence IEA];
                     GO_process: GO:0006412 - translation [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="50S ribosomal protein L34"
                     /protein_id="HEX9917188.1"
                     /translation="MKRTFQPSRIKRLRDHGFLKRMSTKGGRKVLQRRRGKKRKRLTV
                     KPGR"
     gene            3468..3803
                     /gene="rnpA"
                     /locus_tag="VGB16_05580"
     CDS             3468..3803
                     /gene="rnpA"
                     /locus_tag="VGB16_05580"
                     /EC_number="3.1.26.5"
                     /inference="COORDINATES: protein motif:HMM:TIGR00188.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004526 - ribonuclease P activity
                     [Evidence IEA];
                     GO_process: GO:0006396 - RNA processing [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="ribonuclease P protein component"
                     /protein_id="HEX9917189.1"
                     /translation="MKESLPKVIKKKEEIEETIQQGEKRKGKYLLGFVRPYSQENFSF
                     CILINRKIKKAVERNRIKRLLREIVRKNKEIFRTKGRIALLYISRSAETSYSELENDL
                     KGMFYENDR"
     gene            3787..4017
                     /gene="yidD"
                     /locus_tag="VGB16_05585"
     CDS             3787..4017
                     /gene="yidD"
                     /locus_tag="VGB16_05585"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_006555257.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="membrane protein insertion efficiency factor
                     YidD"
                     /protein_id="HEX9917190.1"
                     /translation="MRMIDKFLQAVLIVIIRFYRYSLSYFFPSSCRFEPSCSEYAISA
                     LRSYGVVKGSWLTIKRLSRCHPFHPGGYDPIK"
     gene            4028..5680
                     /gene="yidC"
                     /locus_tag="VGB16_05590"
     CDS             4028..5680
                     /gene="yidC"
                     /locus_tag="VGB16_05590"
                     /inference="COORDINATES: protein motif:HMM:TIGR03593.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003674 - molecular_function [Evidence
                     IEA];
                     GO_process: GO:0051205 - protein insertion into membrane
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="membrane protein insertase YidC"
                     /protein_id="HEX9917191.1"
                     /translation="MEKKNLLALVLISLLTMGYFIYTGSITPKEPSRPYSAKGGIEID
                     TSSVGTQKSPELKTERGKFAVQDTTTPEKVVKVETDLYRAHFSSRGATLKSFLLKRYK
                     YSENGNQIELIPQDSPGSLGILFSDSTLFSFQVDLDSLILNDVTQRGTITFNGHAKDG
                     TTITKKYTFYNNKYHFNLELEISEDGQAESRRDYALAWSSGLASTEKDLNEDLRYFEA
                     YSMMGQQLRSSKKFKKESGTEMDVLEESNSGQTDWVATKTKYFLASIVPLSKKGSGFS
                     VYGKRWFSGQEGQRLEHKQIAVFLEMPFARDSIAIDSFMVYLGPLDYNKLRSYKIGLE
                     NTVNLGWKIIKPFSIAILWIFVNLHKLIPNYGLVIIIFTILMKIVLHPLTHKSTKSMS
                     KMQELQPRITELKEKHKDDTQRMNQEMMKLYKEQGVNPLGGCLPLLLQMPLFYGLFVI
                     FQSTIELRGAEFAWWLKDLSQKDPYFILPLIMSVAMFWQQKITIRDPKQKMMVYLMPI
                     LFFFLFKGFPAGLTLYWTLFNILSLAEQYYLKAKHASEAVSA"
     gene            5655..7070
                     /gene="mnmE"
                     /locus_tag="VGB16_05595"
     CDS             5655..7070
                     /gene="mnmE"
                     /locus_tag="VGB16_05595"
                     /EC_number="3.6.-.-"
                     /inference="COORDINATES: protein motif:HMM:NF003661.0"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003924 - GTPase activity [Evidence IEA];
                     GO_function: GO:0005515 - protein binding [Evidence IEA];
                     GO_function: GO:0005525 - GTP binding [Evidence IEA];
                     GO_process: GO:0006400 - tRNA modification [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="tRNA uridine-5-carboxymethylaminomethyl(34)
                     synthesis GTPase MnmE"
                     /protein_id="HEX9917192.1"
                     /translation="MPAKQFPHEKKNFKAGSDTIAAISTPPGEGGIGIVRLSGNDSFK
                     IADKLFAGKTLPSQMKSHTVCFGKLMDATSSELLDEVLVTVLRSPNSYTCEDMVEISC
                     HGGGLVLSKILEQTLRCGARLANPGEFTLRAFLNGRMDLAQAEAVVDIIRAKTDSSLK
                     VALQNLEGKLSKKLNHCRERLIDILAEIELGVDFIEEDVEELDRKKITKKITELEIDI
                     KNLLDSYVEGRILREGLNVVIVGSPNVGKSSLLNAFLGKDRAIVTPIPGTTRDTIEEF
                     VSIKGIPVRLVDTAGFHISKNKIELEGIRRAKVEIDKADLVVWVVDLSKKLSENERKL
                     EELISKYKYVVALNKIDIASSRTVKKMLAEFKKRDVMRISALQSIGIKNLRDFIISSF
                     IGKTKSENNLIISNLRHKQLLEKSLENLKRAKESLEKGMSPEFVALDLRMALDSIGEI
                     VGKVVTDDILNRIFSKFCVGK"
     gene            7194..9080
                     /gene="mnmG"
                     /locus_tag="VGB16_05600"
     CDS             7194..9080
                     /gene="mnmG"
                     /locus_tag="VGB16_05600"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003353427.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0050660 - flavin adenine dinucleotide
                     binding [Evidence IEA];
                     GO_process: GO:0002098 - tRNA wobble uridine modification
                     [Evidence IEA];
                     GO_process: GO:0008033 - tRNA processing [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="tRNA uridine-5-carboxymethylaminomethyl(34)
                     synthesis enzyme MnmG"
                     /protein_id="HEX9917193.1"
                     /translation="MIIYKEEYEVIVVGAGHAGCEAALATSRMGHKTLLLTMNKEAIA
                     QMSCNPAVGGQAKGHLVREIDALGGEIGFNTDKAAVQLKVLNKRKGPAVWSTRAQCDR
                     TAYRVAMRQVLESQPNLWVKQGNIIEILADKNKAKGVLTETGTAYLGKILILCVGTFL
                     NGLIHIGLTKFEAGRMGEFPARGITENLIKLGFEAGRLKTGTPPRLDGKTIDFEKTEI
                     QWGDENPTPFSLRTNKLSFKDLPCFLTYTNSDTHKFILKNLDRSPLYSGIIKGIGPRY
                     CPSIEDKVVRFKEKPRHQLFLEPEGYNTNEYYLNGFATSLPEDVQLSALRTVAGLEKV
                     EMTRPGYAIEYDFFPPIQLKNSLETKLIENLFFAGQLNGTSGYEEAAAQGIVAGINAG
                     LKLEEEEAFVLDRSQAYIGVLIDDLITKGTSEPYRMFTSRAEYRLLLREDNADQRLTE
                     YGHKFDLIDKEIYSRVKIKMKNIEEERGRLKRTYTKLSFFNLESDNNKRISLADALKI
                     PGITYENLAKVDKASQSISPHVALQVELETKYEGYIKRQEEEVAKFRRLENMVIPQDF
                     DYQGLSGFKKEALEKLNKIKPVSVGQASRISGVSPGDIAVLLVHLKRHKEGKVSRETK
                     TFLI"
     gene            9296..9799
                     /locus_tag="VGB16_05605"
     CDS             9296..9799
                     /locus_tag="VGB16_05605"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEX9917194.1"
                     /translation="MNEKFYYSKRFLRGMLVLGIGSMFICITTVVLVSIKDLPFISTE
                     NLLYSLQFICFAIVAAGSFRILFKVKSKPAITITDNKIVFGPYIVKRFFGDGRKLELN
                     FGQIISAVVIDNWNIFVRPLFGKRKDIEVKFKIDDDKVRSFHIYLSVVDNPERLIEIL
                     KQHIKFV"
     gene            9973..10641
                     /gene="rsmG"
                     /locus_tag="VGB16_05610"
     CDS             9973..10641
                     /gene="rsmG"
                     /locus_tag="VGB16_05610"
                     /EC_number="2.1.1.170"
                     /inference="COORDINATES: protein motif:HMM:TIGR00138.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0005575 - cellular_component [Evidence
                     IEA];
                     GO_function: GO:0008168 - methyltransferase activity
                     [Evidence IEA];
                     GO_process: GO:0031167 - rRNA methylation [Evidence IEA];
                     GO_process: GO:0046118 - 7-methylguanosine biosynthetic
                     process [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="16S rRNA (guanine(527)-N(7))-methyltransferase
                     RsmG"
                     /protein_id="HEX9917195.1"
                     /translation="MKLETLKVVSSEWGAPLSSEQIAKLSIFVEHLLAWSRRFNLISK
                     KDSNIEAITKHILDSLTLLRFISIPRNAKIIDIGSGAGFPGIPLKIAREDLDFSLVES
                     THKKFLFLKDIAQKLELSNFCIFNQRAEELSGCDEFKNKYDFATAKALTDLTGTVKIC
                     FPFLKKGGALIAYKGEKLEQELKELKQRVREEAFEVTKRETIFISKINLKRNLVAIEK
                     RLDF"
     gene            10686..11447
                     /locus_tag="VGB16_05615"
     CDS             10686..11447
                     /locus_tag="VGB16_05615"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_013121824.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_function: GO:0016887 - ATP hydrolysis activity
                     [Evidence IEA];
                     GO_process: GO:0007059 - chromosome segregation [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="AAA family ATPase"
                     /protein_id="HEX9917196.1"
                     /translation="MGKIIAITNQKGGVGKTTTAVNLASCLAVAEKRTLLVDIDPQAN
                     STSGIGLDKNKLDKTIYEVLINETAIEDVIRKTEIPFLEVAPSHLRLVGAEIELVNMF
                     AREKKLASALEPIKNKYDFIIVDCPPSLGLLTLNTLTAADSVLIPIQCEYYALEGLGQ
                     LLNTIKLVQRHLNPSLKIEGVLLTMYDSRLNLSKQVAEEVRKHFSDKVYKTVISRNVR
                     LSEAPSFGKPIILYDILSTGAENYINLTKEILDHE"
     gene            11440..12291
                     /locus_tag="VGB16_05620"
     CDS             11440..12291
                     /locus_tag="VGB16_05620"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_004893254.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003677 - DNA binding [Evidence IEA];
                     GO_process: GO:0000910 - cytokinesis [Evidence IEA];
                     GO_process: GO:0007059 - chromosome segregation [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="ParB/RepB/Spo0J family partition protein"
                     /protein_id="HEX9917197.1"
                     /translation="MSKTVLGKGIEALIPGLPEDKFEGKGNLIQLEIKKVKPNPYQPR
                     GAFDQTKLEELVDSIRQRGLVQPLVVRRKEDGFELVVGERRLLAAQKAGLNFVPALLL
                     DKLTKQEMMEMALVENIQREDLNPIDQAKGYSRLITECGVSQKELSKRIGKDRSSVSN
                     ILRLLNLPDKIQRYLSEGKLSEGHARAILAIPDKKSQISLAERIVRQGLSVRRAEELV
                     YKRKEARKGTKKYKTSKYSDLEDRLREYLKTAVKISQKQGKGRIEIEFYSEEDLGRIL
                     ELFNLKI"
     gene            12293..13069
                     /locus_tag="VGB16_05625"
     CDS             12293..13069
                     /locus_tag="VGB16_05625"
                     /EC_number="3.4.-.-"
                     /inference="COORDINATES: protein motif:HMM:NF013700.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0046872 - metal ion binding [Evidence
                     IEA];
                     GO_process: GO:0006508 - proteolysis [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="M23 family metallopeptidase"
                     /protein_id="HEX9917198.1"
                     /translation="MPRRYVNFIIVPEDKSEGIKLRLSTLAVKFLLGIILVVVVVMAI
                     MSIFYVKALSIAMLNNELKKENQRLREYNSKVVQMEKELEDYRSFFSRVANLAGVDQD
                     LIAKNGERVAQVLGNTLDSTVDYSIGPQEVDTQVADIPSGLPLGGWISRDFSTEHKAI
                     DIAAKEGTEVKAAADGVVKFVGWDETFGNLIILKHKGDIETYYGHNQKILVKPNQKVK
                     KGETISLSGNTGKSTAPHLHYEIKKDDKSVNPWNYMGKKR"
     gene            13078..13434
                     /locus_tag="VGB16_05630"
     CDS             13078..13434
                     /locus_tag="VGB16_05630"
                     /inference="COORDINATES: protein motif:HMM:NF016408.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="polymer-forming cytoskeletal protein"
                     /protein_id="HEX9917199.1"
                     /translation="MQKRKEKMEGGLNTFVGKGSTINGELEVAGGVRIDGTVKGKVTA
                     SETVIVGAEGIIEADIDTKITVVGGRVSGVITALDKVELQSKSMIIGDITTKNLVVEQ
                     GAIFHGKCNMKEGMTE"
     gene            13727..14584
                     /locus_tag="VGB16_05635"
     CDS             13727..14584
                     /locus_tag="VGB16_05635"
                     /EC_number="3.1.21.2"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_015739710.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003677 - DNA binding [Evidence IEA];
                     GO_function: GO:0008270 - zinc ion binding [Evidence IEA];
                     GO_function: GO:0016888 - endodeoxyribonuclease activity,
                     producing 5'-phosphomonoesters [Evidence IEA];
                     GO_process: GO:0006281 - DNA repair [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="deoxyribonuclease IV"
                     /protein_id="HEX9917200.1"
                     /translation="MSLLGAHMSISGGVFNSLLEGEQVGCNTVQIFTKSSNQWQAKTL
                     SANEIDRFYKEQERTKINPVVAHNSYLINLGSPDRGLLEKSRESMLIELGRCGRLSIP
                     YLVIHPGSHLGDGEKKGIKRIADSINWLLGKTAKDEVKIGLETTAGQGSNIGYRFEQL
                     AEIMGLIEDKKRVGICYDTCHTFAAGYDIRNKESYEKTWKEFDRILGTKNLLAIHMND
                     SKKGLGSKIDRHEHIGKGEIGLAGFKLIMNDQRWENVPKILETPKKEDKLKLDKMNLA
                     TLRRLIVKK"
     gene            14939..16831
                     /locus_tag="VGB16_05640"
     CDS             14939..16831
                     /locus_tag="VGB16_05640"
                     /inference="COORDINATES: protein motif:HMM:NF025910.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="tetratricopeptide repeat protein"
                     /protein_id="HEX9917201.1"
                     /translation="MKRIWFLLILVLFFWWRGSYSMVLLDIKVEEGKKGKVESFSLID
                     QGTVELYDSLETSAFLGNFTLSAQAATFDSVNLTLDLSLFTLGPDIQSYFKEYLLKTG
                     EETVLPELRVKDDILFRVKITPHIKAEETNRCNLNTKDAETWYSDPSTHFYYHYILET
                     LPDYHWNTNKGVLEDFYHRIWDRFGFWDPDKIDYFLVPCELKEIYWDKRYGQGLDPTK
                     RKIYVIYNQSTRSVDGPGVGMFLFYRYWGYAPAMVVEGATGNIGLAHYQAQKLKKRGM
                     LFSLKELEISKDYKSKPKEIAFYQASSFIKYLVDTYGLGRFKKFYEEVTDLTFEQALF
                     KVYGKKLPQLEKEWLAYLDTFKEYEANLVYFVTLKTQYRHYAEAIDLLNDLLEMSENK
                     AGYLSDLAGIHYTLGDYEKAKEYYLKLLDLKPENPLFICLLGNTYNLLGDYGQAESLY
                     VKAIGLDTSYITPLLKLGELYLAEGEYQKGKELFDKGLSKGPGTEEAALLFLGSAECK
                     KNLGQPEEAQEDLEKGLYQCQVMLNSFPERPAPYYYSGKVYLAMGFPDTAAYYLETAA
                     TLEDIPYRRGKILLDLAGAYSAVGDLELAQKAYQEILDINCGAREKEQAEKLLAQPKE
                     RKEGSK"
     gene            16828..17415
                     /locus_tag="VGB16_05645"
     CDS             16828..17415
                     /locus_tag="VGB16_05645"
                     /inference="COORDINATES: protein motif:HMM:NF014573.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0009055 - electron transfer activity
                     [Evidence IEA];
                     GO_function: GO:0010181 - FMN binding [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="flavodoxin family protein"
                     /protein_id="HEX9917202.1"
                     /translation="MRVLAISGSPVNGGNVEVLLEEISMGAESRGAKFSLIHLNDLEI
                     LPCQACGKSLEPNFCFYADGMGLVYEKLLACDILVLGSPIYFDSLSAQTKLFIDRCNC
                     IRPIVETPKGDFIFKNRFKKQRKGVAVLVGGKRGRFEGALTVLRGFFKWGNFKLVDSL
                     IYKHDDWEVGGVKNNKEVMKRAFELGEGLAQGKAK"
     gene            complement(17832..18629)
                     /locus_tag="VGB16_05650"
     CDS             complement(17832..18629)
                     /locus_tag="VGB16_05650"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEX9917203.1"
                     /translation="MMDEEDYFEEKRFHQNIQKSIDLSKEKIKANPKDAWAPLFLGNS
                     YGYLAVYEGKRGKLWSALKDGLRAKSNLKKAIDLDSTLSDAYLGLGSYYYWSSIVTKN
                     FQWLPFLGDKRKEGIEFLKIAATKSIFSKEAATNALIWVYINEGWYPLAVMHSKDMCE
                     KYPQGKLFLWPLASAQYQSGDWEGAYASYSQLLVKIEKSQPKNYYNLIECRTKMANAL
                     YNLGKMEECQKECEKILSYPLDEKLRERQKNNLELVKNLKKKCEQKM"
     gene            complement(18835..19851)
                     /locus_tag="VGB16_05655"
     CDS             complement(18835..19851)
                     /locus_tag="VGB16_05655"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEX9917204.1"
                     /translation="MKSKILAILLFLFLYPFTLYAEDRDSIIFAGIDYIHQDKFDEGI
                     AEFKKLIQLNPDDAGGYMYVAVSFVSIIDDYRNQSYRGEFEKYINLAIQKGEERMKRG
                     NYTAEDLLYLGGSYGYRGIYRSFNGDWWGAFWDGGKAKKILEKGVETDSAYYDIYFGL
                     GAYNFYRSIKSKMLWWLPFFGDKRKLGIEQTKLAIQKGKFTRDEAKYGLLRIYVENQQ
                     YDSALALGPDLKKIKPDDPFLLWFLGQTQIKKGMYKDAQETYQALLKTFGASKYSTPR
                     GMVETKYWIAYLYQVMGDKANCLEQIDQILKYQTQGKSDDHIKEFIQKAQDIKKNIQQ
                     HNQP"
     gene            complement(19856..20833)
                     /gene="ilvC"
                     /locus_tag="VGB16_05660"
     CDS             complement(19856..20833)
                     /gene="ilvC"
                     /locus_tag="VGB16_05660"
                     /EC_number="1.1.1.86"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011343453.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ketol-acid reductoisomerase"
                     /protein_id="HEX9917205.1"
                     /translation="MKIYKDKNANLKYLKDKTIGILGYGSQGRAQAANLKDSGCKVII
                     GLRAKSKNNKLAQKDGFEVLSIEKAVQESDIISFLIPDHTHPEVFSKIQKYLPLGKTL
                     LFAHGFSIHFKLIHPPKNVDVILVAPHSPGKILRENYKNKTGVTGFVGVYQDYTGQAK
                     KLVLAYAKGIGLTKAGVLWTTFRDEAIGDIFGEQAVLCGGLTELLKKGFEVLVQAGLP
                     EGNAYLECVHQLDFIVNLIKSYGISGMYNRISKTAEYGSYLSGKRVIDDKVKQNMRKV
                     LAEIKNGAFARKWIREYKAGMKNYVKIKKQVSSHPLEKTGESLRRKFTG"
     gene            complement(20945..21232)
                     /locus_tag="VGB16_05665"
     CDS             complement(20945..21232)
                     /locus_tag="VGB16_05665"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_013908346.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004518 - nuclease activity [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="GIY-YIG nuclease family protein"
                     /protein_id="HEX9917206.1"
                     /translation="MTKEYYVYIIANQNNRVIYTGVTNDLKKRIYEHKKKLIEGFTKK
                     YNITKLVYYEVSNDIKYAITREKQIKGGSRTKKVAMITKMNPEWEDLYDNL"
     gene            complement(21459..22829)
                     /locus_tag="VGB16_05670"
     CDS             complement(21459..22829)
                     /locus_tag="VGB16_05670"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_013275485.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_process: GO:0006260 - DNA replication [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="replication-associated recombination protein A"
                     /protein_id="HEX9917207.1"
                     /translation="MNKEKSDLFEEEKLKAKIPKGPLADRMRPQNLDELVGQEHILGK
                     DSPLRRAILEDKIQSIILWGPPGSGKTTLAHVIAKTADAHFIPFSAVTSGIKEIKDVM
                     NEATKQRNFYGKKTILFVDEIHRFNKAQQDAFLPHVEKGNITLIGATTENPSFEVISP
                     LLSRCKVYTLNSLTLEQVELISKRALEDNQRGLGNFKVKLSTDALEYIVQTSNGDARV
                     ALSALEFAVLTTKPDKKDQTRKITLEIAQEVMQKKILLYDKAGEEHYNIISALHKSLR
                     GSDVDASLYWLGRMLAGGEDPLYIARRLVRFASEDIGNADPQALQITIAAKEAYHFLG
                     TPEGELALAQAVVYLATAPKSNAIYRAFGLVQEDIQKHQALPVPLHIRNAPTDLMKKL
                     GYGKDYKYPHSFPDHFVNQDYLPENLKNRKYYSPSEFGFEKEIKKRMEWWERKRRGLD
                     KGEDDG"
     gene            23365..23904
                     /locus_tag="VGB16_05675"
     CDS             23365..23904
                     /locus_tag="VGB16_05675"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEX9917208.1"
                     /translation="MEEKFYYTRQFVNSQLIAAILFLVTAILWNVAYSLEGWSVWWRI
                     FGLLYTLLCLHYLTISIIIKNKPSVIITDEKIVARSPFFLKHKISFEQIIPTAIIGEK
                     IKLKYKKPNNKEGSFSIAHLRKIDNCERLVDILKQRKNLYRKVGTGRDLFLQLNQRNY
                     YRICHRQGFFGKTQGAALF"
     gene            complement(23827..24945)
                     /gene="hemW"
                     /locus_tag="VGB16_05680"
     CDS             complement(23827..24945)
                     /gene="hemW"
                     /locus_tag="VGB16_05680"
                     /inference="COORDINATES: protein motif:HMM:TIGR00539.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0051989 - coproporphyrinogen dehydrogenase
                     activity [Evidence IEA];
                     GO_process: GO:0006779 - porphyrin-containing compound
                     biosynthetic process [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="radical SAM family heme chaperone HemW"
                     /protein_id="HEX9917209.1"
                     /translation="MSLSIYLHIPFCLSKCPYCDFASIPLDRNLVEKYVSALLYEIKS
                     FSSLKIDTIYLGGGTPTVLKLEQLDKIMQVIKRDCTLTPNCEITIEANPNTLDENRIR
                     GLRNMGFNRVSLGVQSFNDKELKNLSRIHSADQAIHSFNLLKAGGFENIGLDLIFGIP
                     EQTLRSWKSSLKKAVGLSPQHISTYSLTIEKETKFYQMKKEGKIKLLTDEKVRQMYLW
                     AIDYLTSKGYKQYEISNFSLPGFESKHNLNYWNHKEYLGFGASAHSFYKDKRWGNLRG
                     VRKYIELMKKKGKAIQFEEKPSKEQLITEAIFLGLRKTEGIDLRQFQKDFKVKLEDIR
                     KDKINHLTGLGFLSKQGSSLRLTKKALPVADSVIVSLI"
     gene            complement(25307..25981)
                     /gene="lepB"
                     /locus_tag="VGB16_05685"
     CDS             complement(25307..25981)
                     /gene="lepB"
                     /locus_tag="VGB16_05685"
                     /EC_number="3.4.21.89"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_013909924.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004252 - serine-type endopeptidase
                     activity [Evidence IEA];
                     GO_process: GO:0006465 - signal peptide processing
                     [Evidence IEA];
                     GO_process: GO:0009306 - protein secretion [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="signal peptidase I"
                     /protein_id="HEX9917210.1"
                     /translation="MVQLASKKRNIASPRRRLFVILLLIAIVIFLLGNFVVQIYRVPT
                     GSMEDTLLVGDWVVVNKLAYRFRAPQPEDIVVFRYPLNLTKTLIKRCAATEGQTVEIV
                     GKVVSVDGRMFRDSEKVKFTDKYIIPALYSTRDNFGPIQVPIDELFVLGDNRDKSEDS
                     RTWGFVEKGNLKGKVDLVIFSWAPDPNAPKLRSPYIFPLIEIFFYNLYHIPDRVRWSR
                     IGSRVR"
     gene            complement(26047..27846)
                     /gene="lepA"
                     /locus_tag="VGB16_05690"
     CDS             complement(26047..27846)
                     /gene="lepA"
                     /locus_tag="VGB16_05690"
                     /EC_number="3.6.5.n1"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_017950602.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003924 - GTPase activity [Evidence IEA];
                     GO_function: GO:0005525 - GTP binding [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="translation elongation factor 4"
                     /protein_id="HEX9917211.1"
                     /translation="MKSSNVRNFSIIAHIDHGKSTLADRLLEKTGTVSPHQMREQVLD
                     DMDLEREKGITIKSHAIRMEYKAKDGEIYILNLIDTPGHVDFTYEVSRSLACCEGALL
                     LVDASQGVEAQTISNLYLALENNLTIIPVINKIDLPSAQIETVKKNLIELLGVNVEDI
                     ILTSAKLGKGIDRVLEEIVRRVPPPEGESSSPLRGMIFDSIFDSYRGAIPYIRVKEGT
                     VSKGTKIKFFSHPGEYEVEEIGYLKMKFVPQQKLSAGDVGYIMANLKNVADTKVGDTI
                     TDAANPAIEPLPGFRDIKPMVFSGIYPSLAENFEELKDSIAKLKLNDASLSFEPEKSN
                     ALGFGFRCGFLGMLHMEIIQERLQREYGLSIINTTPNVEYKVTRTDGKVILVDNPAAL
                     PPSNQMEKIEEPYVELVIISPSEYIGNIMKLATERRGIYITTEYADKNRVRLHYHIPL
                     SEIIFDFYDRLKTISRGYASMDYKLLEFRPSKLVKMDILINGTPVDALSIIVHQEKAY
                     YLGKQLCTKLRQLIPRQLFEVIIQGAIGSRIISRERISPIRKNVTAKCYGGDITRKRK
                     LLEKQKEGKKRMRQIGTVEIPQEAFLALLKIER"
     gene            28532..29092
                     /gene="pth"
                     /locus_tag="VGB16_05695"
     CDS             28532..29092
                     /gene="pth"
                     /locus_tag="VGB16_05695"
                     /EC_number="3.1.1.29"
                     /inference="COORDINATES: protein motif:HMM:NF013368.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004045 - aminoacyl-tRNA hydrolase
                     activity [Evidence IEA];
                     GO_process: GO:0006412 - translation [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="aminoacyl-tRNA hydrolase"
                     /protein_id="HEX9917212.1"
                     /translation="MIKLLLGLGNPGRKYSSTRHNLGYRLVELLAKRSKRKFKYGKGS
                     YIFTEVKIKRKKVILVKPQTFMNQSGIAAKDCLDDFSLSPEELLVLCDDMNLPLGKLR
                     IRPKGADGGHNGLKSIISLLNTRDFARLRMGIGLPSPEIRAEDYVLQDFRSIEKDEVA
                     KILKDASSAVETVIFQGITESMNRFN"
     gene            29165..29503
                     /gene="rpsF"
                     /locus_tag="VGB16_05700"
     CDS             29165..29503
                     /gene="rpsF"
                     /locus_tag="VGB16_05700"
                     /inference="COORDINATES: protein motif:HMM:TIGR00166.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0000314 - organellar small ribosomal
                     subunit [Evidence IEA];
                     GO_component: GO:0022627 - cytosolic small ribosomal
                     subunit [Evidence IEA];
                     GO_function: GO:0003735 - structural constituent of
                     ribosome [Evidence IEA];
                     GO_process: GO:0006412 - translation [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="30S ribosomal protein S6"
                     /protein_id="HEX9917213.1"
                     /translation="MRKYETVFVLDSSLDKATTDQEVKRVEDLIASREGKVIKVDRWG
                     IRRLAYPIAKKQQGYYTLIIFEGGSDLLGELERSYKLNESCLRWITVVCEAEEEPAEK
                     SPSKEEKDQV"
     gene            29543..29773
                     /gene="rpsR"
                     /locus_tag="VGB16_05705"
     CDS             29543..29773
                     /gene="rpsR"
                     /locus_tag="VGB16_05705"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_013276970.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0000314 - organellar small ribosomal
                     subunit [Evidence IEA];
                     GO_component: GO:0022627 - cytosolic small ribosomal
                     subunit [Evidence IEA];
                     GO_function: GO:0003735 - structural constituent of
                     ribosome [Evidence IEA];
                     GO_process: GO:0006412 - translation [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="30S ribosomal protein S18"
                     /protein_id="HEX9917214.1"
                     /translation="MEELIRKKGVCRFCEGRAPRIDYKDEKLLRRFITERGRILPRRI
                     TGNCALHQRRLSIAIKRSRHLALLPFTSEVYR"
     gene            29777..30727
                     /locus_tag="VGB16_05710"
     CDS             29777..30727
                     /locus_tag="VGB16_05710"
                     /inference="COORDINATES: protein motif:HMM:NF021506.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF2232 domain-containing protein"
                     /protein_id="HEX9917215.1"
                     /translation="MERDTNSSLKNKVWIWASLCLLLTLGYAFSSGIWVVSYFLGIAL
                     ALCLTIFFWRSKRYGLIGAVFLAALFYALRGGNSVVFLSKCLLPALILSLGLKHKRNP
                     SLIIFLAIIPHLVILGLTVAHYGDIVFIFRDQLGELAQRIAGVFEIFGINAIVLQTKI
                     FSIFDTLAQLIFGFELLSSWVEVFLIYLLIQLIGDKLGWDTIKLTRFGLWHSQERFGW
                     VALLSLVLFLVGGKLFEIVSKNAALVFGFWYTVVGFSISEYFFGKSKVNMWIKILFYV
                     FVLVTQILSFVLLSLLGFFDSWLDFRKLGKKSLSNKGVNI"
     gene            30724..31176
                     /gene="rplI"
                     /locus_tag="VGB16_05715"
     CDS             30724..31176
                     /gene="rplI"
                     /locus_tag="VGB16_05715"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_014559466.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0000311 - plastid large ribosomal subunit
                     [Evidence IEA];
                     GO_component: GO:0022625 - cytosolic large ribosomal
                     subunit [Evidence IEA];
                     GO_function: GO:0003735 - structural constituent of
                     ribosome [Evidence IEA];
                     GO_process: GO:0006412 - translation [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="50S ribosomal protein L9"
                     /protein_id="HEX9917216.1"
                     /translation="MKVILREDIPSLGKCGEVIEVKPGYARNYLIPKNLVIMATKGSL
                     KAINEVSKQKELRENKKKKESLKLKGAIEKISCTAEVNVGEEDKVFGSVTSQDIVSLL
                     KEKGFDIDKKKLELENPIKVLGVYTLPIKLHPEVVANLKLWVVKKEGR"
     gene            31178..31666
                     /locus_tag="VGB16_05720"
     CDS             31178..31666
                     /locus_tag="VGB16_05720"
                     /inference="COORDINATES: protein motif:HMM:NF014618.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="bifunctional nuclease family protein"
                     /protein_id="HEX9917217.1"
                     /translation="MAMVEVKVNGLALDIVTNSPVVILSDIESDRLLPIWIGHYEAWA
                     IAMELSGLASKRPLTHDLIKSAIELMGGKVEKVEVTQLVNQTFYAKVHIQKNGSLMEL
                     DARPSDSIALALKTQAPVFVDEKLFEAKREEMEGKEMGDMESLKERLKRINPEDFGKF
                     SL"
     gene            31915..33762
                     /locus_tag="VGB16_05725"
     CDS             31915..33762
                     /locus_tag="VGB16_05725"
                     /inference="COORDINATES: protein motif:HMM:NF016251.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="penicillin-binding protein activator"
                     /protein_id="HEX9917218.1"
                     /translation="MLKKPFIFLLILAILITAYSIHALAQAPENEFKQALSLYSDGNY
                     KEARVRFEKLISTYPADEHLSIFKFMLSKCDYYLGDYILAQSGFRSFVTEFPQSLYLG
                     DAHFMLGNIDYLEGNKFEAAVEYLKAYDYAENEELKGLAYNSLIPLLKVGLSLSEHDR
                     LVRQSRGRKLADEVLFQLAQRELERKNFAEAKDFLEQYIENYPEGSHLKEAEELLKPV
                     ASPRGEYVSKPTQDIYPRLPSASPRGERPETSKIGVLVPLSGVYQTYGENLLKGIKLA
                     VAKYYPQRIELTVKDTQENPIVTAHKTKELIEENGVIAIIGPLKSDCAASAAAVANCL
                     SVPLVCPAVSEEGITTLGKYIFQLSTSTEKMGRAMAHYATTSMGLTEFVILAPDDSYG
                     RNTVSGFEEEVKHLGGKIAACQFYSRESTDFGNELRAIRDILFKEKEKREGGIDTTKY
                     VDIQGKPLPLDAIPVQVDGFFLPGYSEDVILIAPQIAFTKIETHLLGSEGWKNKRIIE
                     LSGDYVQKAVFVSDSYEDENDSSDFVSSYEKTYAEKPDKVAVLGYDSIKFLGLALEGK
                     SWTSQEIGDCLSKVKDFDGASGKFLLNPQHENESFSIFTIEGKKFKKLE"
     gene            33771..33989
                     /locus_tag="VGB16_05730"
     CDS             33771..33989
                     /locus_tag="VGB16_05730"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEX9917219.1"
                     /translation="MDQQERKVALKVFIDGKERDVTFDELTLSNNLALESLLRVLARK
                     KVVDSKELMEEMERVRKERMPDLPLDKK"
     gene            33992..34429
                     /locus_tag="VGB16_05735"
     CDS             33992..34429
                     /locus_tag="VGB16_05735"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEX9917220.1"
                     /translation="MSEQVMLNWKVHPFKRNKKVSIGVILLLFLVWGIVYLATGSLLF
                     LAIAVVMLLGSLSGFFLPTEYEFYTNKIKVKYFLASLEKEWKNYRSFYIDKNGVLLSP
                     FKEPSRLENFRGVYLRLDRNSEIKDKVIDFIKSKMDNSVDGSR"
     gene            34491..34922
                     /locus_tag="VGB16_05740"
     CDS             34491..34922
                     /locus_tag="VGB16_05740"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEX9917221.1"
                     /translation="MIFRQKFSLPDFKETDSNLKTQNSGNLTPEQEELLRKVAQKVVR
                     WKMSVPAILFLESVKPLNYLGSQMMVFFEPFVQTVFTWKDYDQYRIIMEERENVEKLL
                     QKIEELDAIEKEKEKEGKKKIKEKRKNLPWGKRIWSYISGR"
     gene            35169..35807
                     /locus_tag="VGB16_05745"
     CDS             35169..35807
                     /locus_tag="VGB16_05745"
                     /inference="COORDINATES: protein motif:HMM:NF014068.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DJ-1/PfpI family protein"
                     /protein_id="HEX9917222.1"
                     /translation="MKRILIALLVLVLCFSLTLAQEEKPLSGKKVVMIIAPKDFRDEE
                     LLEPMTILLGKGAEVAVASSSLDTSVGMLGAKMKPEILVSNIKAKDWDAIVLVGGQGA
                     REYWNDKVLHTILKDAVKEDKIIGAICIAPVTLANTGILKGKKATVYEGVADKLKSKG
                     ASYTGSDVQEDGKLITANGPKAAGRFAEVLVEALGKGQKVCPKTGLKTCPLH"
     gene            36159..37985
                     /locus_tag="VGB16_05750"
     CDS             36159..37985
                     /locus_tag="VGB16_05750"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002647622.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="glutamate mutase L"
                     /protein_id="HEX9917223.1"
                     /translation="MKDLKPENVNVILATDCGSTTTKAILIEKKGSGYRLIVRGEAPT
                     TVEAPFEDVTMGVLNAVGEVEELTGRKLLDENGRIISPAKGNIGCDIYISTSSAGGGL
                     QMMVAGVVRTMTAESAERAALGAGAIVMDVISSNDKRLPHEQIQRIRHLRPDMILLSG
                     GIDGGTTTHVVELAELISAADPKPRLGSGYKLPVIYAGNVNAREAVKETLSGKTDLDI
                     VDNIRPVLERENLLPAREKIHDLFMEHVMAQAPGYKKLMTWTDAPIMPTPGAVGLIIK
                     IIADLEKIEAVGVDIGGATTDIFSVFQGVFNRTVSANLGMSYSVSNVFAETGLPNVMR
                     WVPFHMDERDLRNRVKNKMIRPTTIPQMMEELIFEQAIAKEALRLAFIQHKSFATVLK
                     GIQQQRTIADAFDQTASGQTLVNMMSLDILVGSGGVLSHAPRRQQAALMLIDSFLPEG
                     ITRLAVDSIFMMPQLGVLSTIHPKAAKDVFDKDCLIHLGTCIAPVGGGKEGKEILKYK
                     FKLPSGKEEEGVLKYGEMKRILLDVGEKAEASLEPYRGFDIGKGKGAKVETKISGGVA
                     GIILDGRGRPFSLPTDDKIRVNKLKEWMLALDIYPKEALESL"
     gene            38375..39502
                     /locus_tag="VGB16_05755"
     CDS             38375..39502
                     /locus_tag="VGB16_05755"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_020384107.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEX9917224.1"
                     /translation="MGHAYTPGLKVTERVLLIKNRILPLKGEVLVKVGDKVHPDTVVA
                     RTLLPGNVEPVNVANKLGIPAEDVEFHMLKKEGDKVEEKETIAESRSFFGLFRSDVHS
                     PIVGKIESVSHITGQVLIRGNPIPVEIKAYLEGEVVEVFEKEGVAVSTWGSFIQGIFG
                     IGGETHGQIKVLTKDNKEVLTDKLIDKSCKGKVIIGGSIVTSNAIHQAISVGACGIVV
                     GGLDDKDLREFLGYDLGVAITGSEDLGITLVITEGFGQINMAEKTFELFKSKEGKEAC
                     INGATQIRAGVIRPEVVIPYEGDVHKKEEKENLGNVGLTTGYPVRVIRQPYFGELGKV
                     VALPPQLQKLESESKARVLEVQFKDGRKVVVPRANVEMLEE"
     gene            39865..40644
                     /locus_tag="VGB16_05760"
     CDS             39865..40644
                     /locus_tag="VGB16_05760"
                     /EC_number="2.4.2.59"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_013644139.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003824 - catalytic activity [Evidence
                     IEA];
                     GO_process: GO:0009228 - thiamine biosynthetic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="sulfide-dependent adenosine diphosphate thiazole
                     synthase"
                     /protein_id="HEX9917225.1"
                     /translation="MKLDDIKISRAIIESYKDKLVRALDVDVAIVGAGPAGMVCGYYL
                     ACAGKDVVIFERKLSVGGGMWGGGAMFNQIVIQGKAKKIMDEFDIHTELYEKGYYIAD
                     SIEAVSTICSKTAKAKVKIFNLLSAEDVLVKDKRVCGLVLNWTAVEMAGLHVDPITMR
                     ARFVVDATGHAAEIVHIIERKSGMKLKTATGKIQGEQSMWAEAGEDAILGNSKEVASG
                     LYVCGMCANAVFGAPRMGPVFGGMLLSGEKVARELIGKLCQ"
     gene            41072..41545
                     /locus_tag="VGB16_05765"
     CDS             41072..41545
                     /locus_tag="VGB16_05765"
                     /inference="COORDINATES: protein motif:HMM:NF013499.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="OB-fold nucleic acid binding domain-containing
                     protein"
                     /protein_id="HEX9917226.1"
                     /translation="MTDSNHFNLKNCLIGLAALTVAILIILYAVKVWLVDKAPSSHSS
                     DSGSITLGSSSFEKRSSKAITWQEADKHYGENVTIEGRIAGTYNSGKVCFLNFDTNYK
                     NSFTAVIFASDFHLFPANPESYYDGKKVRIKGRIKEYSGRPEIILNNLTQIEVIK"
     gene            41762..43015
                     /locus_tag="VGB16_05770"
     CDS             41762..43015
                     /locus_tag="VGB16_05770"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEX9917227.1"
                     /translation="MGRKLCATLLALTLSLIPAAGRSSDFVLSSFVEAGSWSTTQDCE
                     EDLDETFSYQYYHLRFEEKLSTKSKYSLSSFLYHKNYTSLDSLDNLSRTLNFLWSYDA
                     VKSGAKTFSLDLALGYRGKRYEDNKAFDYDKVYILPSAVFVLKDNLKLDFSFGVENFN
                     YLSGKNKDEFKLLGAIGCERFFEKKQLKLGLGYKIENSSKSRQGRKKLKQDVSIGAVL
                     KPKASLLSELALKMTFGQRDTKDDEDQDQDYDYCYRSFLIRTKHKLKKKVTFGFECGY
                     FKKTYVSALLGYHSYHIANNWDYQILDNEKHKVTLGLWLEHKSVDYIGGQRSDYRKET
                     IKTKVNFQTKKEWKLGAGLEENFYIYQTPAKDKNRTYFVFSLEKAFFSRRFLLQADFK
                     TRFTDYKYQNDQEQKAVRLGIEYRF"
     gene            43363..44136
                     /locus_tag="VGB16_05775"
     CDS             43363..44136
                     /locus_tag="VGB16_05775"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEX9917228.1"
                     /translation="MKEKVIAKKTLSIKEIDLITRLEFYGKEIYTKGEIVSFCGGSKP
                     AAYLIKKLLEKNRLRKIIKNVYLFVPMKAPGGLWAGNEYLIAKALARGARYYIGYSAV
                     FNSYGFTDQVAQTMHVINDRYSMRKTVFGVRYKLIKVLPNRLYGLETRKIDNEDVVFP
                     KKERAMIDVFEFYNVRKAYSILTDQISELNTPLLINYLTQYPVLGVRRRVGYFLEKLR
                     INRDLLSKIKVGEKGYSPLYDIGSNQGKINNRWRIVING"
     gene            44129..44992
                     /locus_tag="VGB16_05780"
     CDS             44129..44992
                     /locus_tag="VGB16_05780"
                     /inference="COORDINATES: protein motif:HMM:NF020419.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="nucleotidyl transferase AbiEii/AbiGii toxin
                     family protein"
                     /protein_id="HEX9917229.1"
                     /translation="MDRDKLARIIPVIAQEYNFRLEIIEKDFYLTLILNSVESHLSDK
                     IVFKGGTLLNKIHLNYHRLSEDIDFTYLSPDGLDTRSKRSHALAPIREKMPELLKLLD
                     LKSEKPEGQGFNNSTQYVFNISYPSLISGKDENIQLEISLRQTPIDKPVHNVIAHFYK
                     DPFTGEDLIPKNKILSLSLNEAVAEKLKASITRKDAAIRDYYDLWYISETKFDFEDKK
                     FLYIFKKKLESEKYRANYSYNFGLDQKKVELLHKQIETELIPVIHVGESFDLDKVFRR
                     FNRILQKIWRA"
     gene            45327..45773
                     /locus_tag="VGB16_05785"
     CDS             45327..45773
                     /locus_tag="VGB16_05785"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEX9917230.1"
                     /translation="MMKMTKIRDKLDLIRGEIISSTARVEFILGYKLRKYFFPKANNK
                     ATILFWNVINTPFLNFDNKISLYESIPYFKKLKGHSEIKKSLRFIQKLRNKMAHWELD
                     EKESDLKNIVMFTLVGKYRKITINDSSIEDYRKHVNFLLNKFGYSR"
     gene            45900..48716
                     /locus_tag="VGB16_05790"
     CDS             45900..48716
                     /locus_tag="VGB16_05790"
                     /inference="COORDINATES: protein motif:HMM:NF014516.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016887 - ATP hydrolysis activity
                     [Evidence IEA];
                     GO_process: GO:0006302 - double-strand break repair
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="AAA family ATPase"
                     /protein_id="HEX9917231.1"
                     /translation="MDKGAHFYRCDFQVHTPRDLNWAGPEAVTPEERKTYAEELILVC
                     RSKGLDAIAITDHHDFAFFPYIKQAAKDELDDNGQRVSAHKRIVVFPGIEVTLTAPNC
                     QALLILDADFPENLLPSVLTALAITPAPAEHSKHADVQRIPQNVVSDLTDLYQKLNSH
                     QQLKNRFIVLPNVSETGCGTMLRSGFGNFYKSMPCVGGYTDGPVSQFGTGNLSIVRGE
                     NREYGFKAIGIFQTSDNRKRNHEDLGKYTTWVKWSEPTAEALRQACLAKESRISQEVP
                     LLPPVFVTSIDVSNSKFMGQIYLEFSQQYNAIIGGRGTGKSTILEYLRWGLCDQVSEI
                     VQDEGEIPGYQEKRRKLIEKTLLPFDSTVQISFVKNNVPHILRRKSGTNEMSLKIGSG
                     EFQPCGEEDVRNLLAIQAYSQKQLSSVGVSIEELKRLIYSPIRQTLNEFDLKFEKLRS
                     DIRSCYELWQQKKLIASEIEKGELELKSLTEQVEGLRQGLKGISDQDKATIASHKQYE
                     IVEQHVDGWHGELATAREALETIKRELAEYPTTVPDDVKLPDTELIVVTDIQKTLKDI
                     FDEINATVDSLGDRLNPKGEVLTKLNDLIDSWKGALNQHKRQYESAKQKSTSQESTLK
                     QIQIIEDRVKEIRRSLTERKQAMLKTGDPDTKFNVLKEEWFNAHKEKADLFQAQSEKL
                     NELSDDNLKATLGRGKGTVNLENVLKEIISGSNVRKEKIESLCQRIKEATDPIQQWNQ
                     ILAEFEAIAHFDPTKTPAATLPATPILSSIGFSANDLRKIAEKINSENWIDLFLVQLD
                     DLPLFEYRTREGEYIEFTDASAGQQATSLMRVLLNQEGPPLIIDQPEDDLDNRMVSDI
                     ATLIWKSKKTRQLIFTSHNANIVVNGDAELVVCCGYKVTGDQSKGEIKNQGAIDIEEI
                     REEIIEVMEGGEAAFKLRKEKFGF"
     gene            48767..49081
                     /locus_tag="VGB16_05795"
                     /pseudo
     CDS             48767..49081
                     /locus_tag="VGB16_05795"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_010245751.1"
                     /note="incomplete; partial in the middle of a contig;
                     missing C-terminus; Derived by automated computational
                     analysis using gene prediction method: Protein Homology."
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="type I restriction-modification system subunit M
                     N-terminal domain-containing protein"
     gene            49270..50178
                     /locus_tag="VGB16_05800"
     CDS             49270..50178
                     /locus_tag="VGB16_05800"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_017742739.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DNA adenine methylase"
                     /protein_id="HEX9917232.1"
                     /translation="MIIAERQVKPFLKWAGGKGQLIEQVVSYLPSSLKEGRIKKYFEP
                     FLGGGALFFWLAKQYEFRSSYLYEINPAVCLCYQVIKKNVRKLVKELQQLELEYFSST
                     ENRKEQIYYDRREEFNAFLHGKASNGVVHRTALLIFLNKTCYNGLYRVNSQGEFNVPF
                     GRYKKPTICDEDNLHAVSELLQKAEIVCGDFALCLDYADDKSFVYFDPPYRPISSTSS
                     FTSYSKGVFDDAEQKRLRKVYGSLDKKGACIMLSNSDPKNMNPEDNFFDELYNRYRIV
                     RLNATRLINCNANQRGVITEILVMNY"
     gene            50191..50961
                     /locus_tag="VGB16_05805"
     CDS             50191..50961
                     /locus_tag="VGB16_05805"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_009174391.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEX9917233.1"
                     /translation="MANSKSWKKIVDDYEILGHDFDKSPFVLSANQIKRACQEFKETG
                     EKEVRILCKQDNREDRPDVFIENGLFLLPVRNGLYNIIKGEGYVDIPEIKKETAIYSS
                     MLDFKLETTEVGNSEMQHLDYAYASSLIRTFMDDSSLVLTIRGRKYTPEFEFSIGKHQ
                     IKVASVQTEVDAGYEGKNQVVLIEAKNFSATNIIIRQLYYPFRQWQARTKKKVVTLFF
                     DKNPGDNSYSIWHFEFTDSQNYNSIRLVKSGKYRIIEK"
     gene            50979..51767
                     /locus_tag="VGB16_05810"
     CDS             50979..51767
                     /locus_tag="VGB16_05810"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_004104685.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003677 - DNA binding [Evidence IEA];
                     GO_function: GO:0008170 - N-methyltransferase activity
                     [Evidence IEA];
                     GO_process: GO:0006306 - DNA methylation [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="DNA methyltransferase"
                     /protein_id="HEX9917234.1"
                     /translation="MDKKNTDERLEPQTTTVWSFPNRGKWLSHNAKYRGNWAPQIPNN
                     LIQLYSKEGDIVLDPMVGSGTTMIEAKSLGRKGLAFDIHPEVVKLAQDACMFSSEQKH
                     FEPAIEQGDARNLKSLKDHSVDFIATHPPYLNIIKYGRKDVDGDLSAISSLKKFCDEI
                     EKVAGECYRILKPGKFCAILIGDTRRKRHYVPLAFSVMQRFLKPGFVLKEDVIKIQHN
                     CATTRYWNAQIKDFLLIMHEHLFVFRKPDVDEDKFLYKDSIYNV"
     gene            51898..52698
                     /gene="tcmP"
                     /locus_tag="VGB16_05815"
     CDS             51898..52698
                     /gene="tcmP"
                     /locus_tag="VGB16_05815"
                     /inference="COORDINATES: protein motif:HMM:TIGR04474.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="three-Cys-motif partner protein TcmP"
                     /protein_id="HEX9917235.1"
                     /translation="MKEVTSEIDGLVVRSSGIWIKQKYYYLKRYLDIFSKGMKNRWKG
                     NLTYIDLFAGPGRCLIESIKEEEDGSPLIALQYDFKKYVFVEESKELIEALKTRCKNS
                     PKFSRIEFLEGDCNNVVDEIIPQVDPAGLNLIFIDPTGIDIHYKTIERIVKNNRSDLL
                     INVQLGMYITRNFLAYKKKGDNSKLGLFLGGNVDWDKLKNPQDAVKLYKERIKGLGYQ
                     TVEFKDITIKNTLNAPMYFLLFASKNPKGLHFWKEITKKDHSGQFELF"
     gene            52749..53531
                     /locus_tag="VGB16_05820"
     CDS             52749..53531
                     /locus_tag="VGB16_05820"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_009052218.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003824 - catalytic activity [Evidence
                     IEA];
                     GO_function: GO:0051539 - 4 iron, 4 sulfur cluster binding
                     [Evidence IEA];
                     GO_function: GO:1904047 - S-adenosyl-L-methionine binding
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="radical SAM protein"
                     /protein_id="HEX9917236.1"
                     /translation="MEKIYRKSLLYKSEVEYADFCLNHVEGCSHGCKYPCYAYMMKKR
                     CGIVKSYDDWCKPKIVGNALDLLDKEIPKYKSKIKYVHLCFSTDPFMYNQEEVSDLSL
                     KIIDKLNLNGIRCTVLTKGVFPEEVGHKTVFSKNNEYGITLVSLDENFRKKFEPNSAP
                     FRNRIRSLEDIHKQGFKTWVSMEPYPTPNLIKQDLLEILEAVSFVDQIIFGRLNYSVK
                     SSEFGHAKEFYNSMASSVIKFCRKHKIKYHIKDGTQKLSGGQ"
     gene            53698..54405
                     /locus_tag="VGB16_05825"
     CDS             53698..54405
                     /locus_tag="VGB16_05825"
                     /inference="COORDINATES: protein motif:HMM:NF012780.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="CBS domain-containing protein"
                     /protein_id="HEX9917237.1"
                     /translation="MTRADKFLSLFNLIEKTLHKISPREPGEGFKEMINRLSKSNKII
                     CQYKYDLRDYADLRNAIVHQFTGEAIAEPHEDVIANLQTIHDILVRPPTAIEISSKPV
                     YCCEAKDKIVDVVKTMTAKVYTHVPVYEDDRFVGVFSESSIARWLGENAEKDGFLLNE
                     TRVGELKQFFEKDNDPFNSYKFVSKDIDVFTIMDYFLSFVNENKRLGAIFVTNSGRSS
                     EGILGIITSWDLPRIDK"
     gene            54707..55990
                     /locus_tag="VGB16_05830"
     CDS             54707..55990
                     /locus_tag="VGB16_05830"
                     /inference="COORDINATES: protein motif:HMM:NF015451.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="aminoacetone oxidase family FAD-binding enzyme"
                     /protein_id="HEX9917238.1"
                     /translation="MKVNTKPDKLWDVVVIGGGPAGMMAAGRAAELGRSVLLVEKNAI
                     LGKKLLLTGGGRCNLSNNKPELRTMLAKYKGVDQFLFSAFSQFGVEDTLEFFKRQGMA
                     TKEEAEGRIFPVSNKAQTVLDVLVSYMKEGGVELRTNSVVTRLWVDASNTATQHIRIR
                     LKDKTELLAQSCVVASGGTARPATGSTGDGFAWLKKIGHTIIQDDVALVPVALKDAWA
                     KKLSGVTLIDIKLTTFQNKEKQEVGKGELLFTHFGISGPTVLDMSKEIGELLKYGEVT
                     IMLDIFPKLDHGALRDKLQTLLNSQSNKKLKNTMNKLVPLALVPAILRLTGVDGQLPS
                     HSLRREHRIKLVSVMKAIPLNVKGLLGADKAIVCSGGVALEEVDFRTMQSRLVPNLYI
                     VGDLLNIDRPSGGYSLQLCWTTGFVAGSNTERSRG"
     gene            56349..56942
                     /locus_tag="VGB16_05835"
     CDS             56349..56942
                     /locus_tag="VGB16_05835"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEX9917239.1"
                     /translation="MIIRCTARFLKEMGLTKADLTSNTSEESELGEWYTNLFFVERKK
                     CVIFTNARTLFTFIWFDVDRSEIRNLGKLFRIGFGKALLDEDFDGALIQHLVNECRDI
                     RFAKTQDRSVLGVMVDHVKNTKWMVIRDGGLEKSDFSEIIKQLNRTPLLTKDFTYSIE
                     ELGKVLGVAVDTKLNFNPSNTFLSSSAPEIRDNKEQA"
     gene            57159..58208
                     /locus_tag="VGB16_05840"
     CDS             57159..58208
                     /locus_tag="VGB16_05840"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_006421716.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="Fic family protein"
                     /protein_id="HEX9917240.1"
                     /translation="MPFNPKFTITAKINKALVEIERVRGFLDALKLKDDWFIDMQQKA
                     LILESHHSTHIEGTALSLQQAKSILEGKKANGVRADDKRELTNYKKAMDLVSRYLGKD
                     DPITESAISGLHKVTVKSVRGGQADPGNYRKIQNYVVNSRTRKVIYTPPGPLTVPLLM
                     REFVDWLNKTEEVSPILVAGIAQFQFVHIHPFVDGNGRMARLLSTLILYKTGYDFKRL
                     FTISEYYDKDRPSYYKAIQSVREGNMDMTGWLEYFVCGLRSQMEEIQAKGQQLIKQDV
                     KLQKIKKLGIGLRQEKAVKHLIIKGTITVNEYQEIASCVRRTAQRDLEDLVDKGIIQA
                     VAKSATDPTKYYVLL"
     gene            58694..59002
                     /locus_tag="VGB16_05845"
     CDS             58694..59002
                     /locus_tag="VGB16_05845"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEX9917241.1"
                     /translation="MELALKIIGMFLGVLARTYVPYLRKLKEGKETKFKKCYTLSTVA
                     SLVLGLIITLLVFPQFDIAKAGVGLEASIKLFCLAFGFGFGWNSIVSETIKWTTGGKN
                     "
     gene            59225..60385
                     /locus_tag="VGB16_05850"
     CDS             59225..60385
                     /locus_tag="VGB16_05850"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_020254131.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005515 - protein binding [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="fibronectin type III domain-containing protein"
                     /protein_id="HEX9917242.1"
                     /translation="MVLSKKMNFLFILIIFASSCSLVWADQPPIPPTEVKGSDTKNDA
                     GGSITLSWKRSPDDGAGKNNIVSYKVLRSSQEEGFYILISDVTAGAEQFLDNNAKDGI
                     KYFYKIKAISNEGFSLSLPSPAIVSKAQWFDTTKIYILAMSVILFSAILYFISKAKSG
                     KELFIRKIAGLDAVDEAVGRATEMGRKIIYIPGINDLDDIQTIAGITILGRVAKLVAE
                     YEAKLEVPVSRSMVMVSCRETVKEAYINAGRPDAYSDDMVHYLTDDQFAYAAACDGIF
                     VREKPAAIFLMGTFFAESLVLAETGNSVGAIQIAGTARPAQLPFFVAACDYTLIGEEL
                     FAASAYLSKDPKLLGSLRGQDVGKVLFLLGILVGTILISFNILDISFIFNVR"
     gene            60404..61057
                     /locus_tag="VGB16_05855"
     CDS             60404..61057
                     /locus_tag="VGB16_05855"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEX9917243.1"
                     /translation="MRREVPIAITAVVGIIFLMSYFTPHFPLNVLHNSATDIFMIIAA
                     FSILLGVLNLVRVNLEKISKKKRDWPFSIVLILGLLVMTIVGLGFSKGRNFMELGTNF
                     RYLYDYVYNPLSATMFSILAFFIASASYRAFRGRNLEATLLLVAAFVVMLGRVPIGYY
                     LSSWLPTNYHLGDAASWIMNVPQKAGQRAIMIGIALGVVSTSIRIILGLERTYLGGE"
     gene            61079..61930
                     /locus_tag="VGB16_05860"
     CDS             61079..61930
                     /locus_tag="VGB16_05860"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEX9917244.1"
                     /translation="MNFFEKLLVIDRRVIFLFVAVAIAVPLFFKVNLKVDITPEVRKV
                     YDIMENLPEGSKVLVAFDYDPGSMPELQPMAESFIRHCIRKKLKMIIMGLWPQGFIQA
                     NQALEKVLKEEEFKDVKLEEGVDWVNLGYAAGNEVVIERMGSDISAVFAADYRGMKLG
                     NLPLMKGVKNFSNIDFIYNLSAGYPGTQEWVQFAVDKFGAKLAAGNTAVQAPLIYPFY
                     PKQLLGVLGGMKGAAEYEVLMGIPATGVKYMFSQSVAHGVVCFFVIIGNLAFFLTRGK
                     RKSGGQK"
     gene            61927..62595
                     /locus_tag="VGB16_05865"
     CDS             61927..62595
                     /locus_tag="VGB16_05865"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEX9917245.1"
                     /translation="MSEAIGLWIGALLTLGIFSFLYKDNPLYKFCEALFVGISAGYFV
                     VISYWQIIVAKMVDVLRVGFDYWIVLRYFDFQSWLPLLGGVFGILMLLRLFPKIGWIS
                     RWSLALVVGTTAGLYFTVYLVSNGMMQVKSTIIPLYVAGDMSTTIGNIVVVLGTVCGL
                     IYFFFSKEHKGVFGGVAKIGIWFIMITFGASFGYTVMSRMSLLIGRMDYLGISFGEFL
                     KLLK"
     gene            62710..63897
                     /locus_tag="VGB16_05870"
     CDS             62710..63897
                     /locus_tag="VGB16_05870"
                     /inference="COORDINATES: protein motif:HMM:NF042826.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005515 - protein binding [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="fibronectin type III domain-containing protein"
                     /protein_id="HEX9917246.1"
                     /translation="MKKRRSYPLILFLLLGFLLIWVIAFAQEESPLPPSQVKVKDTPN
                     DNGHSLTITWKSSPDSAKILGYEILRSDSPDGEFKVVGQALATDTLYKDKGDKEKESP
                     NYFPSHKNFYYKVRAKTDGLSSESEISLPAYAFQQWYHAGKTPVLVAVLIFSTLTLIF
                     ISKAKKGKELYVRPLAGIGAVDEAIGRATEMGRPILFILGLGTAADIATIAGFTILSR
                     VAKRTAEYKTRIIVPCNDPIVMAVAQETVKAAYMDAGRPEAYEEKNIFFLTSMQFAYV
                     AGVNGIMLRERTATNFYMGLFYAESLILAETGSIAGSIQISGTDQMSQLPFFVAATDY
                     TLIGEELYAASAYLGREPLLLGALKAQDWAKGILLICLILGTIFTSLHLDFFANLFKF
                     SLE"
     gene            63901..64551
                     /locus_tag="VGB16_05875"
     CDS             63901..64551
                     /locus_tag="VGB16_05875"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEX9917247.1"
                     /translation="MRRQLPILVVFISGIFMAIQYFVPSEPAEFVQEYAMDWVIVIGV
                     FAMGLGLWSLTKVNWIKVQRQTPGWGYNLVTILGMVFMLVVGLIWDPGLGFFPGEKSQ
                     VSGTWFMNFFWYIYLPIQTVMFSLLAFYIASAAYRAFRARTILASILLVTAIVIMLRL
                     IPLGAVSGINQWLANWILIVPNMAAKRAIWMGVGLGMVAMALKIVLGIERSYMGRD"
     gene            64555..65412
                     /locus_tag="VGB16_05880"
     CDS             64555..65412
                     /locus_tag="VGB16_05880"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEX9917248.1"
                     /translation="MGFYEKLMSLDRRWVYLCVAIAVVLPFLLEEFGVPIFIPPYITP
                     EVKSVYNFVEKLREDDVLFLAIDYDPMAKAELHPVARAVLKQCFKKNVKVIISALSQN
                     GPGMAEQLISQTAKEYNKVDGVDYVYLGYKPFPALVIMGMSQNFKLFFPKDYYGTPLD
                     SLPMMAKVRNIKDTKGVIDVAAGSVADMWINEGAGRYNYPLALAVTGVMAADYYPFLN
                     SGQLFGLVSGMKGAAEYEYLMEKGGYMKKEEFDASRGMPIQMFAHIVVILFIIIGNVG
                     YFMSRRKKS"
     gene            65427..66158
                     /locus_tag="VGB16_05885"
     CDS             65427..66158
                     /locus_tag="VGB16_05885"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEX9917249.1"
                     /translation="MSLVDFMWMSIGALLTLFTFSYLYKDNPFYKFAEHLVVGVSAGY
                     FAVLLYYSSFMPKVIDTVFRPIEWKYFILAFKQIKPEYLLAALKGSQWWYLVPGILGV
                     MMWTRFSRKLSWISRFSLAFYMGIGIGASIPLTLQNYVLKHLTSTIQPVNFTSLTGIW
                     RLLIVILVFCTLVYFFFSREHKGAFGASAKVGIWVLMIAFGAGFGLTVMGRVALLAER
                     VIFLRDYWVELKYFLYMLFKYGMLL"
     gene            66177..66980
                     /locus_tag="VGB16_05890"
     CDS             66177..66980
                     /locus_tag="VGB16_05890"
                     /inference="COORDINATES: protein motif:HMM:NF042826.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF6754 domain-containing protein"
                     /protein_id="HEX9917250.1"
                     /translation="MISKIIFYLSFWFDFNRVNVLVTLLVFFSFLIYFIGRAKKGKSL
                     FIRRIAGLDAIEEAVGRATEMGRPVLYIPGIEDMDNIQTVASMIILGNVAKMAARYET
                     TLIVPICRPFVVPVAEESVQQGYMAAGRPDAYDKNSVRYISDEQFAYTAGVNGIMLRD
                     KPAANLLFGSFFAESLIMAETGFSTGAIQVAGTANIHQLPFFVVACDYTIIGEELYAV
                     SAYLSKEPKLLGTLKGSDLAKLIIMIILILGAILATANVEWFRNLFFVQ"
     gene            67348..67968
                     /locus_tag="VGB16_05895"
     CDS             67348..67968
                     /locus_tag="VGB16_05895"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEX9917251.1"
                     /translation="MRREVPLVICFLFGTLFIADYFISNPGFDSLAHTINQWAIIVMI
                     FAYVLGVANIARINSGVIKRKEKDWAYKLVLLFGLLFMAILGIGWGVTEGTVFYWVYM
                     NAYYPMQATMFALLAFFIASAAFRAFRIRSWEAGLLAITAIAVMIGRVPVGEAVWKNF
                     PNFTEWIMDAPQLAAKRAIMIGAALGAISTGLKVLTGLERTYLGGD"
     gene            68317..69162
                     /locus_tag="VGB16_05900"
     CDS             68317..69162
                     /locus_tag="VGB16_05900"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEX9917252.1"
                     /translation="MKEKLIKIFEALEKMDRRIIFIFIGLAVILPFFIPFGFEFSVSP
                     PVQKFYDSIGSIPPGSKVLLSADYDPASMPELYPMHLAALKQLFSRDLKVICTQLWPT
                     GSPLAEMAFQEIAVGEFDKKYGVDYVNLGFKEGREVVMVSMGESIPKTFPTDYHGTKV
                     EDLPIMQGVKNFNDVAMIVNISAGVPGTKEWVLQVQSRYHIKLASGTTAVSAPEFYPY
                     LQSNQLAGLLGGMKGAAEYEKLVGRIGLGRRGMDSQSVSHIVIFIFILIGNIAYFSMG
                     GRKKK"
     gene            69159..69854
                     /locus_tag="VGB16_05905"
     CDS             69159..69854
                     /locus_tag="VGB16_05905"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_020250985.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEX9917253.1"
                     /translation="MIHTEIIGTWLGAALTLFIFSFLYKDNPFYKFAEHLFVGVSAGY
                     GVAMEFHNVFVPNLWKPLWGGNLWFLVPFAFGILLFTRFFSKIAWLSRWSIALIIGIF
                     AGIYIVGYAQGDLVQQIHANLLPLWQGNLFSFIRNFLSAFYNWLTFRGSVLIWLDYTK
                     NFLLVVGVLVSLVYFFFSKEHKGALGVGAKVGVWFLMVSFGASFGYTVMSRMSLLIGR
                     VYFLLGNWLGLIR"
     gene            complement(69913..72057)
                     /locus_tag="VGB16_05910"
     CDS             complement(69913..72057)
                     /locus_tag="VGB16_05910"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEX9917254.1"
                     /translation="MKRNSFLKATVLFLMFLASVALLFSCAKDKSPTAGSGAAQTIVT
                     NLSASPADSTLVGGSISIYATVTTGGQIDTGEIVNFSVSPSYVGTLSATVDTSEEHGL
                     VVTSFHGNNTGTAYVYASTSDTTASIRVRVISSGVTPGEGGLTIEVDPTVLPSNGDTT
                     SEVTITVRDANGLPVTDGTIVRLAAGEKFKDVDGDGYYNKDIDQLIYDTNNDGVWNSI
                     GNIPMTAATTSGVATATYTTGFRAGMVYIKATAGSPDDPVTDDATILLIPTTGDSIAS
                     IALEPASPTIQVKGTGGIEFVQMTATCYDENGNQVVEGWPVVFRILNGPDGGEGINGS
                     TSACTVSTNSSGIAQVTVNSGTKSGTINLIAYFGNVISNSTRITVCAGPPKFISVGVR
                     PCNIRGFDIVNAEAEVGAVVVDSFGNYVPNGTSVYFYTDEGMITPNDETEEGVASATY
                     RSAPTSGDTGIAWIRVETAGGTIKDSVILIVSGAPYYVDFLSYPSSGIPATSKASYTI
                     FAGVQDINRNPVLAGYKVDFEATYGTIGDSKTDSSCIPGDYAFASSKYTSINLDVDYA
                     WSTPDNGIGANEWVWAGCGGAVGTVTFPLTTGSSYKDETSNYSRVIADNEIDFNSLGS
                     FTVRITDVFKNPLGGHRINVINISNGTISGSDIDITDSYGLANFIFTTPVYDSTVSRK
                     AVNITVADTSDARGGIILSGRINLIQPTPDST"
     gene            72213..72467
                     /locus_tag="VGB16_05915"
     CDS             72213..72467
                     /locus_tag="VGB16_05915"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEX9917255.1"
                     /translation="MKKIVSVFLVLLLSVGSSFARGDTLDFRVKTDAGKSKQNYPAIA
                     ASGQGRTVAVWQDYRSGNNYDIYVQLFDSSGIKIEGKVRL"
     gene            72969..74774
                     /locus_tag="VGB16_05920"
     CDS             72969..74774
                     /locus_tag="VGB16_05920"
                     /inference="COORDINATES: protein motif:HMM:NF045524.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0005576 - extracellular region [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="T9SS type A sorting domain-containing protein"
                     /protein_id="HEX9917256.1"
                     /translation="MKRIIFWLFLSFFIFSVSFAVEFKVSKEYEETLIRQVEELYTNQ
                     EPIYPLPKCGTPIMAEISLLKEKLSSEALELLKFYLARPSLQRSYNTPGGHFKIHYDI
                     TGTNTVYHPSEDTNPANGIPDYVDRCAEIFDYVWAKEIDTMGYKAPPSDGSMGGDSRY
                     DIYIINLGYGYLGGTSPESPRTPSWSYTSYIELTNDFSGYGYASQYDLMSVTAGHEFF
                     HAIQFGYDVYEGYDPYPNWKPYWMEMSSTWMEDQTFDNVNDYPNYLRFFYRYPWVSLK
                     AFSNDFGADSVRAYHPYASCVFAIYLSEKFGVGVMKDIWEECGRVPDANTLPAIDSAL
                     APFGADFDSAFKEFLVWNLFTGTRANTVNYYSEGNLFPEINILNEQKHSTYPVNVTSV
                     DSMPENLASNYVRFTPLSGPGGLDMTFNGADNAEWVVPVVGYKSGGGNSIGAFSLNAQ
                     GEGNFDFYNWDDYDYVYMTPGVLTRDSSKYNYAYADTFDINLGVDEDQLPDRMEYILP
                     QNYPNPFNSSTNIPFTVYGSQFTVDSRIHTTLKIYNILGQEVRTLVDEPKRPGNYEIT
                     WDGKDNENRDLPSGVYFYKIETEGFVQSKKMLLLK"
     gene            complement(74776..75132)
                     /locus_tag="VGB16_05925"
     CDS             complement(74776..75132)
                     /locus_tag="VGB16_05925"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_007219979.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_process: GO:0000160 - phosphorelay signal transduction
                     system [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="response regulator"
                     /protein_id="HEX9917257.1"
                     /translation="MPKILIVEDEPNLLELYTKEMRDEGYEVLTAMDGEVALDLVKKQ
                     LPDLVILDIKIRKMDGLKVLHEIKQFNKDIPVILNSAYETFKSDFSSWVADAYLVKTS
                     NLDQLKLNTKKLLQLN"
     gene            complement(75142..76779)
                     /locus_tag="VGB16_05930"
     CDS             complement(75142..76779)
                     /locus_tag="VGB16_05930"
                     /inference="COORDINATES: protein motif:HMM:NF014567.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ATP-binding protein"
                     /protein_id="HEX9917258.1"
                     /translation="MEESKHQFHCPTPYSSNTTCWLRLAIQQADLSELPLDKNLKNCL
                     DCQTFNQLSLRAKGRRQADQTSNYFTKFLFSQLDSYNDQLKSTDGSLRKRLEELNVLK
                     KVSDALLKSDDLDKSLLLILSGATAGQAFGFNRAFIFLINEKRNVLEGKMALGPQDNN
                     EAEKIWEELKSKNITFDQIIHNILKGEGKPENELIKKTKKIALSLDSQDNILAWALSD
                     KKSLNLKSFPPNQVGNNKLAQILSPCGIVVVPILSEGKPLGVLVADNLITAEPILDED
                     LFALETFANQAGSEIENMLLHRELRLKYQELEHINQLLRENQSYLLKHERLADIGKLA
                     TTAAHEIKTPLIAIGGYARRVLKNYTSGKLDLRDLEIIIKEVERLEEITSQILDYSRE
                     SKLSIQRNDLNRIVQEALEVLESKFKYDNILLQTVLSDDLKETDLDSHRIKQVLFNLI
                     ENAIDAMFGGGTLTIRTKKKDNYAVLEIEDTGSGINQNEMKKLFIPFYSTKPTGSGLG
                     LAVSKKIVDDHAGFIEVQSKPNEGTRFTIHLPLNQKS"
     gene            76891..77751
                     /gene="nadC"
                     /locus_tag="VGB16_05935"
     CDS             76891..77751
                     /gene="nadC"
                     /locus_tag="VGB16_05935"
                     /EC_number="2.4.2.19"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_012583119.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004514 - nicotinate-nucleotide
                     diphosphorylase (carboxylating) activity [Evidence IEA];
                     GO_process: GO:0019363 - pyridine nucleotide biosynthetic
                     process [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="carboxylating nicotinate-nucleotide
                     diphosphorylase"
                     /protein_id="HEX9917259.1"
                     /translation="MNWQDRKFKALIERALAEDVGTGDITSEAVIDKSLMNCAKIKVK
                     QSGVLAGIFLAEQVFKTVDRKIKFKLYKKDGSQIKKGETIALVCGKTRSILQAERVAL
                     NFLQKLCGIATLTNEFVKKVKKTKVKILDTRKTTPTLRLLEKYAVRMGGGKNHRFGLH
                     DMILIKDNHIRACGSVKQAVLRAKSFIAQSKPKRKIKIEVEVRNIRELEEAIKSGVDR
                     VMLDNMHTGDIRKCVKLVRSSKKKIEIEVSGNVDLNNVIKIARTGVDFISIGALTHSA
                     LALDLNLVLC"
     gene            77934..78920
                     /locus_tag="VGB16_05940"
     CDS             77934..78920
                     /locus_tag="VGB16_05940"
                     /EC_number="6.3.4.15"
                     /inference="COORDINATES: protein motif:HMM:TIGR00121.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004077 - biotin-[acetyl-CoA-carboxylase]
                     ligase activity [Evidence IEA];
                     GO_process: GO:0036211 - protein modification process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="biotin--[acetyl-CoA-carboxylase] ligase"
                     /protein_id="HEX9917260.1"
                     /translation="MVDLLSNNAEKVLKVLKRKKGRFLATRKIAASAGLSSFNVYQSI
                     YHLQSWEYQIESQKGKGYRLVKPTDLLLPFEIKDNLHTKFLGQKIHSYQTLKSTNLLA
                     YKLAEAGSPEGAIVISEKQTAGKGRMGREWFSPSKVGLWMSLVLRPDIPPSKAAGLSL
                     CAGLALAQTIQNLFGLKAKIKWPNDCLINGKKVAGILLELSAEIDQIRFVIMGVGINI
                     NQKRSDFPSGLQDKATSIRIELGQRVRRLDTLKSFLEKFEKIYLVFRKKGLGTYRQDI
                     KKYSSVLNKEIRIKSGDEVITGKAYDIDDNGSLVIKTKDGTRAIITGEVNLL"
     gene            79213..80550
                     /locus_tag="VGB16_05945"
     CDS             79213..80550
                     /locus_tag="VGB16_05945"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_007909030.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="phosphomannomutase/phosphoglucomutase"
                     /protein_id="HEX9917261.1"
                     /translation="MDESIFKSYDIRGIYPQQLNEDIAYKLGRALVEYLRPQTVAVGR
                     DVRLSSESLACATIKGITEEGAEVIDIGLCSTDGLYFAVGNFKYDGGVMITASHNPPE
                     YNGMKVCKKDAVPLSGQEGLDKIKELVLGNSFPTVKKTGKITRRDIDQDYTSHTLSFV
                     DKGELKPFKIVIDAGNGIAGKIVPQVFRELPCKVIPLFFDLDGTFPNHLPNPIVPENT
                     VSLRDEILKQKADLGAIFDGDADRMFLMDEKGKLLGGDMVTALVAKNLLKKEKGATIL
                     YNLVCSKTVPEIIQKSGGKGIRTRVGHALIKPLMKKYNAIFGGEHSGHFYFRNNWFAD
                     SGLIALLVCLELISEENKPLSTLLASIDHYFRSGEINSQVADIPGKLKEVEKRYAGSG
                     KIDRLDGLTIEFDTWWFSLRASNTEPLLRLNIEATSEELLGQKKKEMLELIRS"
     gene            80701..81471
                     /locus_tag="VGB16_05950"
     CDS             80701..81471
                     /locus_tag="VGB16_05950"
                     /inference="COORDINATES: protein motif:HMM:NF012301.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_process: GO:0000160 - phosphorelay signal transduction
                     system [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="response regulator"
                     /protein_id="HEX9917262.1"
                     /translation="MNKPIRVLVVDDDAKIRELLLDTLSALGYEALGARDGEEALSLL
                     EDEKIDLVVTDIKMPKLSGVALLQEIKEKNPELPVVLITGYNSIYPMEEMINQGASGF
                     ISKPFRIGRIEELIVKVLNAKPEKGGAKGSPTKKILVVDDDEALRSVLEETLVSFGYK
                     VTTASDGQEALEKLKEESYDLVVTDIKMPRMNGNELLKEIKQASPGIPVVIITAYPAA
                     YSQQWIMEEGADGYLTKPFHIDRIDKLIRELTLERTPS"
     gene            81919..82686
                     /gene="kdsB"
                     /locus_tag="VGB16_05955"
     CDS             81919..82686
                     /gene="kdsB"
                     /locus_tag="VGB16_05955"
                     /EC_number="2.7.7.38"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_009164298.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008690 - 3-deoxy-manno-octulosonate
                     cytidylyltransferase activity [Evidence IEA];
                     GO_process: GO:0009244 - lipopolysaccharide core region
                     biosynthetic process [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="3-deoxy-manno-octulosonate cytidylyltransferase"
                     /protein_id="HEX9917263.1"
                     /translation="MKKIKVAGIIPARFASARMPGKPLRSVWGEPLIQRVYQQARKSA
                     FIDKLLVATDDHRIMDAVFGFGGEAVLTSAKHKSGTDRAGEAADKLNCDLVVNIQCDQ
                     PFLNPKMLDQLISYMLKNKKIYMATLAQRINQKKDLSDPNVVKVVMDRNGYALYFSRS
                     LLPYSKAENRTYYKHAGIYGFRKDFLAKYAKLAQTPLEKSEKLEQLRVLENGYKIKVL
                     ISKYNSLSIDTPLQLKEINHKSSTSSRDESALKRRRN"
     gene            82683..84314
                     /locus_tag="VGB16_05960"
     CDS             82683..84314
                     /locus_tag="VGB16_05960"
                     /EC_number="6.3.4.2"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_013433610.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003883 - CTP synthase activity [Evidence
                     IEA];
                     GO_process: GO:0006221 - pyrimidine nucleotide
                     biosynthetic process [Evidence IEA];
                     GO_process: GO:0006241 - CTP biosynthetic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="CTP synthase"
                     /protein_id="HEX9917264.1"
                     /translation="MKDKKTKYIFITGGVVSSLGKGIASASIGYLLKQRGLKVNLLKL
                     DPYLNVDPGTMNPFQHGEVFVLDDGAETDLDLGHYERFIDESLTKDNNVTSGQIYDSV
                     IAHERRGDYLGGTVQVIPHITNEIKSRIRKVGEMNGKADVVMAEIGGTVGDIEGLPFL
                     ESIRQMGLEDGRHNTLYIHLTLVPFIKSAGELKTKPTQHSVKELREIGIQPDILLCRT
                     SLPLTKGIKEKIGLFCNVPSEAVIQAIDLDCIYEVPISFHQEGLDELVIKHLDLECKE
                     LDLFDWKKMLEKIKNPKDKVNIGICGKYTNLKDAYKSIIEAFVHAGVENNAEVELKWI
                     ESDNIIKEGKEKYLEGLDGLLVPGGFGDRGIEGKIEAIKYVREKGIPFFGICLGMQCA
                     TIEFARNVCNLKDTHSSEFDSQTKNPVIHLMPEQEKIANKGGTMRLGAYPCVLEKNSL
                     AHKIYGEDQISERHRHRYELNNDYREKLTSAGLKLSGLSPDKRLVEIIELPAHPWFLG
                     VQFHPELKSRPTRAHPIFRKFVGAAVKYKKLKNKN"
     gene            84328..85161
                     /gene="kdsA"
                     /locus_tag="VGB16_05965"
     CDS             84328..85161
                     /gene="kdsA"
                     /locus_tag="VGB16_05965"
                     /EC_number="2.5.1.55"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_018248153.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008676 - 3-deoxy-8-phosphooctulonate
                     synthase activity [Evidence IEA];
                     GO_process: GO:0009058 - biosynthetic process [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="3-deoxy-8-phosphooctulonate synthase"
                     /protein_id="HEX9917265.1"
                     /translation="MPEIALRQYNLKIGKNNPLVLIAGPCVIESEKLVLKTAEEIKRI
                     TEKHKIPFIFKSSYSKANRLDISSFAGPGLDKGLEILKKVKDKVGVPILTDIHQPDEA
                     VPVSQIADIIQIPAFLCRQTDLILAAAKTKKPVNIKKGQFMAPEDMKQIADKVVSTGN
                     KNILITERGTTFGYHNLVVDMRSLVILRNLGYPVIFDATHSLQLPGQEGKSSGGQPEF
                     ILPMARAAVACGIDALFVETHPDVEKALCDGKNMLPLEKLENLIVQVWEIDKLIKQFE
                     K"
     gene            85151..85672
                     /locus_tag="VGB16_05970"
     CDS             85151..85672
                     /locus_tag="VGB16_05970"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_013451553.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="HAD-IIIA family hydrolase"
                     /protein_id="HEX9917266.1"
                     /translation="MKNKTLFSKFKNIKLIVSDVDGVLTDNTIYIDDNGTESKKFSIA
                     DGVGAYLAKKFGLEIALISARKSKATEIRAKQLKIKYVFLGREDKLRIYEKIKKDLKL
                     RDSQIAYLGDDIVDIPVLKKAGIPICVKDASTLIKKYADYITKAEGGRGALREVVEMV
                     LLSKNKNLTEIFK"
     gene            85669..86628
                     /locus_tag="VGB16_05975"
     CDS             85669..86628
                     /locus_tag="VGB16_05975"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_013451554.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016853 - isomerase activity [Evidence
                     IEA];
                     GO_process: GO:0005975 - carbohydrate metabolic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="KpsF/GutQ family sugar-phosphate isomerase"
                     /protein_id="HEX9917267.1"
                     /translation="MSLKKAREVIRKEAKAIASLEGKLTKEFSRAVDLILNCRGRVIV
                     TGMGKSGIICKKIAATLTSTGISAIFLHPAEGIHGDLGLVQKSDIVIAITKSGETDEL
                     YQLIPLFKRLGVPIITLTGNTKSPVAEKSDVVIDVSVDEEACPNNLIPTSSTTAALVM
                     GDALAMALLDRRGFSLDDFALLHPGGNLGKRLLLKVSDIMHTGNKVPIVNQGANMKEV
                     VLEMTSKRFGATTVVNSKKELVGIFTDGDLRRLIEKSENIFKFKAKEVINKNPKTIHA
                     EELAAKALNQMEHYKITCLIIIDKKRKPIGIVHMHDLLEAGVV"
     gene            86853..87770
                     /locus_tag="VGB16_05980"
     CDS             86853..87770
                     /locus_tag="VGB16_05980"
                     /inference="COORDINATES: protein motif:HMM:NF015251.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="lysophospholipid acyltransferase family protein"
                     /protein_id="HEX9917268.1"
                     /translation="MKTRIIKGIKNWLIYQFILLGIALFNHLPRKTALKCGSFLGKMC
                     YFLIVKARRQTIENLKIAFKEKNEGEVKRLSLEVFENLGKNAVDAIRLKNASWNEVSR
                     IVEVEGIEHFDRAYKLGKGLVALTGHIGNFELIATYFALAGYKVSVIGRELYDKRLDR
                     LLVENREKAGIQNIPTTAQAREVIKALKSGRALGILIDQDSFRVRGIFVDFFGRPAKT
                     PIGPILLTQRTGSPIVPLAIVRKEDDNYKMIIREEIKTETPEDKEKGLMELTQKGTKF
                     LEEIIREYPDQWVWMHNRWASQPKDETFD"
     gene            87763..88308
                     /gene="lptC"
                     /locus_tag="VGB16_05985"
     CDS             87763..88308
                     /gene="lptC"
                     /locus_tag="VGB16_05985"
                     /inference="COORDINATES: protein motif:HMM:TIGR04409.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0005886 - plasma membrane [Evidence IEA];
                     GO_function: GO:0015221 - lipopolysaccharide transmembrane
                     transporter activity [Evidence IEA];
                     GO_process: GO:0015920 - lipopolysaccharide transport
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="LPS export ABC transporter periplasmic protein
                     LptC"
                     /protein_id="HEX9917269.1"
                     /translation="MIKKRIFLFLVFSLTFFFCCSQEEEKKSPTLADVPDQVVEKTQM
                     VFTVDGVRSTVIGADYVVKYERKDLTLAKKVHVDFFDKEGKHTSIMDADSGIIKEKRQ
                     ELEALGNVVVISDNGVKLETESLRWDPRKQKVITDDFVKVTKEGDVIYGYGLEADQKL
                     ENFQIKKRVKGTIKKVPEGEL"
     gene            88298..89041
                     /gene="lptB"
                     /locus_tag="VGB16_05990"
     CDS             88298..89041
                     /gene="lptB"
                     /locus_tag="VGB16_05990"
                     /EC_number="7.5.2.5"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_005967566.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0009898 - cytoplasmic side of plasma
                     membrane [Evidence IEA];
                     GO_component: GO:0043190 - ATP-binding cassette (ABC)
                     transporter complex [Evidence IEA];
                     GO_function: GO:0015221 - lipopolysaccharide transmembrane
                     transporter activity [Evidence IEA];
                     GO_function: GO:0042626 - ATPase-coupled transmembrane
                     transporter activity [Evidence IEA];
                     GO_process: GO:0015920 - lipopolysaccharide transport
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="LPS export ABC transporter ATP-binding protein"
                     /protein_id="HEX9917270.1"
                     /translation="MNYSLDKDALRAENLVKVYHKRKVVSGVSIEVRPREIVGLLGPN
                     GAGKTTTFYMIIGFVRPNSGKVLIGEEELTSLPMYKRAQKGIGYLSQEPSVFRKLTVE
                     ENIMAILEMQKISKKERKEKLKSLLGELGIAHLAKSKAYTLSGGERRRVEITRALVTQ
                     PKFMLLDEPFAGIDPIAVEDIQKIIFELKQKGLGILVTDHNVRETLSVTDRAYIMCDG
                     EILKSGTSEFLASDPEARKIYLGERFKLN"
     gene            89102..90526
                     /gene="rpoN"
                     /locus_tag="VGB16_05995"
     CDS             89102..90526
                     /gene="rpoN"
                     /locus_tag="VGB16_05995"
                     /inference="COORDINATES: protein motif:HMM:TIGR02395.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016987 - sigma factor activity [Evidence
                     IEA];
                     GO_process: GO:0006352 - DNA-templated transcription
                     initiation [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="RNA polymerase factor sigma-54"
                     /protein_id="HEX9917271.1"
                     /translation="MKLELQMRLQQTLAPQLIQSLKMLQMPILRLEQILRQELAINPL
                     LEEMDCDEEEEAPSTDEEEVDPNLNKIDWDEYLGDDHEFRVKPQREESEDRLERVSVL
                     EKTLYDHLIEQLSFSKLTPEEYEIGEYIIGNIDDSGYLSCSLEEIVDSLKGDPALTEK
                     MLGIIQTFDPLGVGARNLRESLLIQLRERGLEGSLAYRIVDEYLSDLDKKSHSQLAKN
                     LGVSFEEVQEAMDLIRTLSPKPTMGRFSPGATPVIPDLIVERVGDGYAIFHNDRNVPR
                     LRINPIYKELIRKQSTSSPEEKKYVKEKLEQARWFLNAINQRRSTMIRVMETVVEEQK
                     DFFEHGMSHLRPLTMQAVAEKLGLNVATISRVANDKYVQTPQGVFQLRYFFNTGVQKQ
                     DGEELSRLNVKQMLEEIIKKEDPLQPLSDQEIYTRLKEKGLNIARRTVTKYREELKIL
                     PARFRKRMVKEKLENPSSLELEEQ"
     gene            90613..91569
                     /gene="glpX"
                     /locus_tag="VGB16_06000"
     CDS             90613..91569
                     /gene="glpX"
                     /locus_tag="VGB16_06000"
                     /EC_number="3.1.3.11"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011342095.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0042132 - fructose 1,6-bisphosphate
                     1-phosphatase activity [Evidence IEA];
                     GO_process: GO:0006094 - gluconeogenesis [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="class II fructose-bisphosphatase"
                     /protein_id="HEX9917272.1"
                     /translation="MERNLALEFVRVTEAAALECARWMGKGDSNSADEAAVRAMRKMF
                     NSVDIDGTVVIGEGERDKAPMLYIGEKVGTGKPPKVDIALDPLECTNSVACGRWNALS
                     VIAVTESGNFLHAPDTYMEKIAVGPEAKEAIDLNLSPEENLKRVAEVLDYHIEDITVA
                     ILDRPRHEDLVKRVRKAGARIQLISDGDVSAAIATALPDTGVDVLMGIGGAPEGVIAA
                     AALRCVGGGMQARLVPRNEEEERRAKKMGVKDINKIFREDELAGGKNIMFAATGVTDG
                     DMLKGVRFFGGGAYTHSIVMRSKSGTIRFIETTHFFDRKPKY"
     gene            91587..91958
                     /gene="raiA"
                     /locus_tag="VGB16_06005"
     CDS             91587..91958
                     /gene="raiA"
                     /locus_tag="VGB16_06005"
                     /inference="COORDINATES: protein motif:HMM:TIGR00741.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0005840 - ribosome [Evidence IEA];
                     GO_component: GO:0022626 - cytosolic ribosome [Evidence
                     IEA];
                     GO_function: GO:0045182 - translation regulator activity
                     [Evidence IEA];
                     GO_process: GO:0009386 - translational attenuation
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="ribosome-associated translation inhibitor RaiA"
                     /protein_id="HEX9917273.1"
                     /translation="METRITARHFDLTPELKEFCEKQISRLERYFDHIIDCHLILDLE
                     KSRMTAELKLKVHGTALNSKESSFDMHISVEKVLSKIEVQLKKYKAKLKDKKPRKTKA
                     IKQELNAKALACELEEERPSF"
     gene            91972..92940
                     /gene="hprK"
                     /locus_tag="VGB16_06010"
     CDS             91972..92940
                     /gene="hprK"
                     /locus_tag="VGB16_06010"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_020251187.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004674 - protein serine/threonine kinase
                     activity [Evidence IEA];
                     GO_function: GO:0016791 - phosphatase activity [Evidence
                     IEA];
                     GO_process: GO:0009401 - phosphoenolpyruvate-dependent
                     sugar phosphotransferase system [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="HPr(Ser) kinase/phosphatase"
                     /protein_id="HEX9917274.1"
                     /translation="METITVEKMYQERKEHLELALLTNSDGLSKRIRNNEIHRPGLAL
                     AGFIDRFAHNRTQVLGETEMAYIQSQDEKNKRQTLESFFRFDMPCVVITKGINPPVEL
                     IQIANQNVTSVFQTRLSTTEFITRLSVYLDNLFAPHITIHGTLVDIYGVGLLYTGKSG
                     IGKSECALDLVERGHRLVADDVVKVIKKAPHVIVGTGSELLGHHMEVRGIGIIDLEKL
                     FGIRAIRVQKRIEVEVRLVLWEDEEEYERLGIEDKFTTVLGVEMPVVTIPVSPGKNIT
                     VISEVIAMNHMLKVYGQDIAKAFSRRLSDEIYRKKMSNEYLESDTE"
     gene            92946..93326
                     /locus_tag="VGB16_06015"
     CDS             92946..93326
                     /locus_tag="VGB16_06015"
                     /inference="COORDINATES: protein motif:HMM:NF015567.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:1902495 - transmembrane transporter
                     complex [Evidence IEA];
                     GO_function: GO:0016301 - kinase activity [Evidence IEA];
                     GO_process: GO:0008643 - carbohydrate transport [Evidence
                     IEA];
                     GO_process: GO:0009401 - phosphoenolpyruvate-dependent
                     sugar phosphotransferase system [Evidence IEA];
                     GO_process: GO:0016310 - phosphorylation [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="PTS sugar transporter subunit IIA"
                     /protein_id="HEX9917275.1"
                     /translation="MFDCIILTHGNLATSLKDTVEQMMGKQVGMMIISNQGKDPEGLL
                     SKLEELVSKSRSKELFLFVDLYGGSCWQVAKKFSSKRGDVVLITGVNLPMLVQFLSKR
                     EKLDKFELLEKVVESGKGGIVTEF"
     gene            93333..93815
                     /locus_tag="VGB16_06020"
     CDS             93333..93815
                     /locus_tag="VGB16_06020"
                     /EC_number="2.7.1.-"
                     /inference="COORDINATES: protein motif:HMM:NF015769.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008982 -
                     protein-N(PI)-phosphohistidine-sugar phosphotransferase
                     activity [Evidence IEA];
                     GO_process: GO:0008643 - carbohydrate transport [Evidence
                     IEA];
                     GO_process: GO:0009401 - phosphoenolpyruvate-dependent
                     sugar phosphotransferase system [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="PTS sugar transporter subunit IIB"
                     /protein_id="HEX9917276.1"
                     /translation="MIFFRLDERLIHGQVVTGWANALKLDRIILANDQAARNPWEREL
                     YLACLPPEIEGSILTVEEATKYLSNGEFKNRKMILIVNSPADILKMKEGGIQITELNI
                     GGMYQKQGRIQILPYLHLSLEEIADLKKLFSQNVKCICLDVPLGEKRNLKELFGKLNL
                     "
     gene            93830..94729
                     /locus_tag="VGB16_06025"
     CDS             93830..94729
                     /locus_tag="VGB16_06025"
                     /inference="COORDINATES: protein motif:HMM:NF014523.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="MlaD family protein"
                     /protein_id="HEX9917277.1"
                     /translation="MASDRSVELRVGIVVMAAIILLILGIIWVKQYRFNVVRYEYSVI
                     FPNVGALEKGDPVTISGVKKGEVEQINLYEGDVLVAFNLTQDVVLKEDAKFSVMNIGL
                     MGERYIEIFTGHSDKLLDLSKPVRGYYDTGIPEIMGMMGEAVTDIRNLVSHLEGAIGT
                     KWSQESIIEITRNLRKVSNDLNQMMEKNKDKFNRSVDDLSYASSELKNLVKDNKGKLQ
                     SAVDNFANASNRLDSVTTSLQEVSTSLKSFTQKIEKGEGTMGKLVQDSSLYLDLRKTT
                     QDLDSLILDIKANPKKYIKLKVF"
     gene            94733..96880
                     /locus_tag="VGB16_06030"
     CDS             94733..96880
                     /locus_tag="VGB16_06030"
                     /inference="COORDINATES: protein motif:HMM:NF020660.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003824 - catalytic activity [Evidence
                     IEA];
                     GO_function: GO:0030246 - carbohydrate binding [Evidence
                     IEA];
                     GO_process: GO:0005975 - carbohydrate metabolic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="alpha-amylase/4-alpha-glucanotransferase
                     domain-containing protein"
                     /protein_id="HEX9917278.1"
                     /translation="MKKIKFSFAIHDHQPIGNFEFIFRNAHNRCYLPFLELLEKYPFF
                     RISLHFSGILLEWIKNKEPKTLDLIRRLVQRGQVEMVGGAFYEPILSVIPDEDKLGQI
                     RKLSKFIRDEFKCDVCGMWLAERVWEPHLPKPIAEAGIKYTVLDDTHFRYSGLKDEHL
                     LGYYITEEQGHKISLFPGSKTLRYAVPFKEPEEIINYLKSIADEDGKNLIVYADDGEK
                     FGLWPGTYKHCYEDRWIEKFFKEIEKNLDWIEMIHLKEALDEIPPLGRIYLPVASYEE
                     MMQWALPAEVFEEYEDLEKILREKQLFEKYQVFLRGGYWRNFLSKYPESNQIHKKMLW
                     VREKLNTIKPSSQAQRKKLDQAQDFLWKGECNCPYWHGVFGGLYLPHLRSAIYQNLIQ
                     AERVLDDLIHNKGDWIDYKVFDFDKDGSDEIIIESPKLNLYFHPHWGGALYELDYKPK
                     PFNLLDILSRRREGYHRKLLELKKNINKDNQVESIHDIFVAKEEGLEKFLNYDWYRHG
                     SLIDHFLGENAKLEDFSKSKYGEQGDFVNQPFVPKIEKQKSKIILSLKRDGFVWVGKN
                     RIPISLEKKIKISSKTSELNIVYSLTNNSNQEIQLWFGVEFNFGMLSGEDTKRYYYIL
                     GQNPENSKLNSVGQSADTGEFGIKDEHLGLNINLKVDKSAELWRFPIQTISLSESGFE
                     KNYQSSVLFPNWKINLAPKETWEVSIIQEISNL"
     gene            complement(96875..97330)
                     /locus_tag="VGB16_06035"
     CDS             complement(96875..97330)
                     /locus_tag="VGB16_06035"
                     /inference="COORDINATES: protein motif:HMM:NF014037.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="Mut7-C RNAse domain-containing protein"
                     /protein_id="HEX9917279.1"
                     /translation="MKFICDDNLGKLAKLLRTLGYDTLFEKDLDDYKLIRQSLEQNRV
                     ILTRDTKLARFKIAQNQLLITSDNPLEQLKQVIEYFELKPKKEGFFSRCLLCNNILEE
                     VEKDKAKDKIPPYVFKTQKDFVICNKCDKVFWKGTHIDKIRAKLRRVGL"
     gene            97746..98537
                     /locus_tag="VGB16_06040"
     CDS             97746..98537
                     /locus_tag="VGB16_06040"
                     /EC_number="3.1.3.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_012646908.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008934 - inositol monophosphate
                     1-phosphatase activity [Evidence IEA];
                     GO_function: GO:0046872 - metal ion binding [Evidence
                     IEA];
                     GO_process: GO:0006020 - inositol metabolic process
                     [Evidence IEA];
                     GO_process: GO:0046855 - obsolete inositol phosphate
                     dephosphorylation [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="inositol monophosphatase family protein"
                     /protein_id="HEX9917280.1"
                     /translation="MKKDKAERLKFRQTAVKAALEAGEILLENRGKRNKIGYKGEVNL
                     VTEVDFLSEKAILRIIRKNFPDHSILTEETIGQRRDSPYCWIVDPLDGTTNYAHGFPV
                     YCISIALQKDKEVILGAVYNPLLKELFLAEKGKGAFLNGKRIFVSKTKKLSQSLLATG
                     FPYDIRTSNENNLDYFNKFAVKAQAIRRAGSAALDLCYLAMGRFDGFWELKLSPWDTA
                     AGILIITEAGGKTSDFSGKKFGIFSKKLVASNNWIHREILEVINS"
     gene            99105..99740
                     /locus_tag="VGB16_06045"
     CDS             99105..99740
                     /locus_tag="VGB16_06045"
                     /inference="COORDINATES: protein motif:HMM:NF012804.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="class II aldolase/adducin family protein"
                     /protein_id="HEX9917281.1"
                     /translation="MKREDSIRADIVAVCQRIYQNGFVAANDGNVSVKLSDSRILVTP
                     TGRSKGSLTREELLIIDSKGKMLKGNLKPTSELPLILYAYQKRKDIKATVHAHPPYST
                     SLGLAGIGLPKDVLPEVFLTLGEVPLAKYGTPSTAELANSVSDYIKDHDAILLKNHGV
                     LTLGKSVEEAYYKLERVEHFAHILWLAIGLGRVDRLSKKDMNKLIKLKKPR"
     gene            99752..101947
                     /locus_tag="VGB16_06050"
     CDS             99752..101947
                     /locus_tag="VGB16_06050"
                     /inference="COORDINATES: protein motif:HMM:NF027787.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF5050 domain-containing protein"
                     /protein_id="HEX9917282.1"
                     /translation="MKTLLKCLFVVLIASVVGFLFGGCSKKTTSPTTPGEITVTGKVD
                     LPSGASIEPESLVVLSPVNSDAIGSDSSFSANLPPNTAIGVMCITDKNDDPLLLGYIF
                     TSGFNPTFPKVKSAYKTNIISNSGEAVTINSQTTALSLIMLSPLLIGSSPEQRNEFAQ
                     RAVDHQDFPSLVSDIDNMIVNNPENYLTDSTKSPQIFQKAASISLDVLRSFEQESLMK
                     AQKTLRANPSIEDAAGTKVNFINPYYIFYGTGIYDYSTSDEIDVFVQSPRKLFWGTIP
                     YLGPDGKTEYDLGNGDFNIQIYKGFHWFPLNLSDPKCIATWYNTGQGLLNILDLIVCI
                     PGVTNSISTIGIIVQNASDFKGAVELGAALREGDTFEAILGFLRFISSNTYQILVSVL
                     QSSSTPENISKYLNVTKNVAGNVTIALRALNAATKIPFFLDIAYLAPPELEYDVSQVD
                     GNLGFINRPLGKIAFYSTRDGGYDKIYVMNADGTQQTRLTDNTSSNQHPSWSPDGDKI
                     AFVSHRDGYSEIYVMNADGTQQTRLTNSPSGDYWGNFHPSWSPDGQKIAFMSDRDGNW
                     KIYVMNADGSGQMNLTNNTVWEMYPCWSPNGEKIAFTRAGQMLGIYVMNADGTQQTRL
                     TDNPYFFNDQYPSWSPDGQKIAFTSTRDGYSEIYIMNADGSEQTNLTNNDSGDGSPCW
                     GPDGEKIAFESYRDGNWEIYVMNADGSEQTRLTFNSITGHYDGCPSWTR"
     gene            102680..103837
                     /locus_tag="VGB16_06055"
     CDS             102680..103837
                     /locus_tag="VGB16_06055"
                     /inference="COORDINATES: protein motif:HMM:NF012382.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0030170 - pyridoxal phosphate binding
                     [Evidence IEA];
                     GO_process: GO:0009058 - biosynthetic process [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="aminotransferase class I/II-fold pyridoxal
                     phosphate-dependent enzyme"
                     /protein_id="HEX9917283.1"
                     /translation="MIKRIIIDKSDRLQRIAPFPLLELERAKRRLKRINGELIDLGEF
                     SGGLKSMPGLSLDKIEHLDSSFVSNLKKKIALWFENKYGVKLNPEREVFPFVGRKRII
                     TKLTLVFLNEGEVGLVPNPGNPIYRTAIILADGKPEEFPLLERNDYLPNLSYVLNKNK
                     RAKLIFLNYPNDPTSSLADLNFFGEAVEIASQNNILLVNDATYSQIYYDEHSPCSLMQ
                     IKGAKKVGVEIYLFRAPLGEVGFLVGNREIISGLVQLDKALDDKDYLMLRQLDSILDD
                     YPGNINSYNQELAQRKDILYDEFLGLGWKARKPRSTPFFWIKVPPPHTSMGFSRLLLR
                     KAGVVVFPGSSFGEEGEGYIRIALNVPVDGIKQACRRIKEHSHLWQKRYSP"
     gene            104084..104449
                     /gene="folB"
                     /locus_tag="VGB16_06060"
     CDS             104084..104449
                     /gene="folB"
                     /locus_tag="VGB16_06060"
                     /EC_number="4.1.2.25"
                     /inference="COORDINATES: protein motif:HMM:TIGR00526.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004150 - dihydroneopterin aldolase
                     activity [Evidence IEA];
                     GO_process: GO:0006760 - folic acid-containing compound
                     metabolic process [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="dihydroneopterin aldolase"
                     /protein_id="HEX9917284.1"
                     /translation="MGIIRLHNMTFYGFHGVSPAEKETGRRFEADVELHLDLDKAAQS
                     DKLKDTVNYTEVYNTVQQLIMENKFSLLETIGVRLAEKILEKFGVEEVLVRVRKKIPP
                     IPGNLDDIEVEVRRERKKE"
     gene            104486..104989
                     /gene="folK"
                     /locus_tag="VGB16_06065"
     CDS             104486..104989
                     /gene="folK"
                     /locus_tag="VGB16_06065"
                     /EC_number="2.7.6.3"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_012310812.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003848 -
                     2-amino-4-hydroxy-6-hydroxymethyldihydropteridine
                     diphosphokinase activity [Evidence IEA];
                     GO_process: GO:0009396 - folic acid-containing compound
                     biosynthetic process [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="2-amino-4-hydroxy-6-
                     hydroxymethyldihydropteridine diphosphokinase"
                     /protein_id="HEX9917285.1"
                     /translation="MEIVYIGVGSNLGDKKSNIQKAIDSIKEKRILREIKASSLYLTE
                     PVGKKGQPDFLNLVIKGKTELTPFELLDSLLEIEKNLGRKRKEKWGPREIDLDILFYD
                     DMVVNQENLVIPHPEIPNRRFVLVSLMELSPDLKHPLLNKNIKQLLKDTKDHSKVEPY
                     GGIYAGE"
     gene            104976..105614
                     /locus_tag="VGB16_06070"
     CDS             104976..105614
                     /locus_tag="VGB16_06070"
                     /EC_number="2.7.1.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011422731.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_function: GO:0019136 - deoxynucleoside kinase activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="deoxynucleoside kinase"
                     /protein_id="HEX9917286.1"
                     /translation="MQENSYIAVEGAIGVGKTSLAKLLTEKTSARLVLEQVEDNPFLD
                     DFYKDRARYAFPAQIFFILTRFRQQQEMHTTDLFYKRVICDYIFAKDRIFASINLASR
                     ELHLYESILSLLEKDIAKPDLVIYLQASTETLMKRIRRRDREYERGMDYQYLQELNEA
                     YNNFFFHYTQTPLLVVNTDRIDFVKNPKHLDDLVEKIKQPHSGTKYYVPFGE"
     gene            105624..106430
                     /gene="panB"
                     /locus_tag="VGB16_06075"
     CDS             105624..106430
                     /gene="panB"
                     /locus_tag="VGB16_06075"
                     /EC_number="2.1.2.11"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_020403375.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003864 - 3-methyl-2-oxobutanoate
                     hydroxymethyltransferase activity [Evidence IEA];
                     GO_process: GO:0015940 - pantothenate biosynthetic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="3-methyl-2-oxobutanoate
                     hydroxymethyltransferase"
                     /protein_id="HEX9917287.1"
                     /translation="MTKQDKVTVKSFIQMKKEGEKIAVLTAYDFFTAKILDEIGMDSI
                     LVGDSANMVFYGAKTTLSITMEQMIYHTKAVSSATKRALVIGDMPFLSYQTSVSEAVH
                     NAGLFLKEGGAEAVKIEGGLEMIDTIKKIIDAGIPVMGHIGLVPQSIHRFGGYHTQGK
                     DQKTRKYLLESAKSLEQTGCFSMVLEAIPIELAKEITQSISIPTIGIGAGVSCDGQVL
                     VINDLLGLFDEFKPKFVRRYAHLSDEIRKACGEYLKDVKAGGFPSTEESY"
     gene            106502..106879
                     /locus_tag="VGB16_06080"
     CDS             106502..106879
                     /locus_tag="VGB16_06080"
                     /inference="COORDINATES: protein motif:HMM:TIGR02436.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="four helix bundle protein"
                     /protein_id="HEX9917288.1"
                     /translation="MKNCNSKFKDDIKKRTYFYALEVMRSLEQLPKNDFSADIVAKQL
                     LRSATSIGANIIEAQAAGSKRDFTNYLRYSLKSANESKFWIALLRDSGKLNRELSNSL
                     LEEAKELANILGKSILTLKNKNL"
     gene            106937..107782
                     /gene="panC"
                     /locus_tag="VGB16_06085"
     CDS             106937..107782
                     /gene="panC"
                     /locus_tag="VGB16_06085"
                     /EC_number="6.3.2.1"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003517257.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004592 - pantoate-beta-alanine ligase
                     activity [Evidence IEA];
                     GO_process: GO:0015940 - pantothenate biosynthetic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="pantoate--beta-alanine ligase"
                     /protein_id="HEX9917289.1"
                     /translation="MKIITSPNRMQEICNKMQRDGKVVGFVPTMGYLHKGHLSLVRVA
                     KKKSDVLVVSIFVNPAQFGPKEDFKRYPRDFARDGDLLKEEGCDYVFAPKTKNMYPES
                     YSTFIEVNGLSSKLEGASRPGHFKGVTTIVAKLFHIVQPDIAVFGQKDAQQVVIINKM
                     VKDLNFKTKIVIAPTVREKDGLAQSSRNKYLSPKQRKDATVLYESLALAKGLIRNGEK
                     DAKTIVNKMRELINKEKGAKIDYIAIANTENLNPIEKIKGEVLISLAVQFGKTRLIDN
                     IKMKI"
     gene            107793..108143
                     /gene="panD"
                     /locus_tag="VGB16_06090"
     CDS             107793..108143
                     /gene="panD"
                     /locus_tag="VGB16_06090"
                     /EC_number="4.1.1.11"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003000684.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004068 - aspartate 1-decarboxylase
                     activity [Evidence IEA];
                     GO_process: GO:0015940 - pantothenate biosynthetic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="aspartate 1-decarboxylase"
                     /protein_id="HEX9917290.1"
                     /translation="MLITICKSKIHRVKVTEADLDYEGSITIDVEILKKADILPYEKV
                     QVVNNNNGARFETYVIPGKKGSGVICLNGAAARLGEVGDILIIISYCELEKEEAAKYR
                     PKIVLVDKKNKPIK"
     gene            108145..108885
                     /locus_tag="VGB16_06095"
     CDS             108145..108885
                     /locus_tag="VGB16_06095"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_020252558.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016779 - nucleotidyltransferase activity
                     [Evidence IEA];
                     GO_process: GO:0009058 - biosynthetic process [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="sugar phosphate nucleotidyltransferase"
                     /protein_id="HEX9917291.1"
                     /translation="MKKLAVIILAAGKGKRMKSDLPKVLHKIDSKPLIDYVISTAREI
                     NPQRIIVVIGHKYQMVKEYLRDPDLEFVIQKEQLGTGHAVMQTEKIFGDFDGTIVVLC
                     GDVPFLKAETIKELIQEHHKARASATVLTTILDDPKGYGRIKRDGNNLVEKIVEEIDT
                     TSQEKKLKEINSGIFCFESKLLFETLHQVDTNNKQREYYLTDVLKLMRDKKLPVAAVV
                     CPCASEVSGVNSIEQLKQMEKASNFRVK"
     gene            108945..109379
                     /locus_tag="VGB16_06100"
     CDS             108945..109379
                     /locus_tag="VGB16_06100"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEX9917292.1"
                     /translation="MKKAIILFVVLGFLATGTASLWAQSVEQSKVSLTGQTKFSSYSL
                     LDPSKIDLHHSYSFAYFSGSQGSFGLGIYTAVLDYRISSPLSLRLGLSYLHQPLGRLS
                     NKTNLNIKQSILPSFELRYEPNEKTFIFLGFSTSNPYFFQTE"
     gene            109471..109962
                     /locus_tag="VGB16_06105"
     CDS             109471..109962
                     /locus_tag="VGB16_06105"
                     /inference="COORDINATES: protein motif:HMM:NF024791.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="LytR C-terminal domain-containing protein"
                     /protein_id="HEX9917293.1"
                     /translation="MPKRSESAHFYKRREKKKQSREVKDWIIILLTLILVVYALSLAS
                     HLTLNKAQGSGTEEPTYLRVQILNGCKESGLAEKVRDQLISKRWEKFAFDVVDVGNFG
                     DSIISQTLILDRKGQDKRVFEVANILGIKKENVLLKRLEDNYLDIDLSIILGADYSRI
                     FKN"
     gene            110009..110362
                     /gene="rsfS"
                     /locus_tag="VGB16_06110"
     CDS             110009..110362
                     /gene="rsfS"
                     /locus_tag="VGB16_06110"
                     /inference="COORDINATES: protein motif:HMM:TIGR00090.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0043022 - ribosome binding [Evidence IEA];
                     GO_process: GO:0017148 - negative regulation of
                     translation [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="ribosome silencing factor"
                     /protein_id="HEX9917294.1"
                     /translation="MAGGLALEKKGLDIRVLDLRKLTSVSDFFVICTASVEVHAKAIA
                     DWIVENLERKGFKVWHNEGYQACRWILLDYVDVVVHIFLKEVREFYGLEKLWGDAPAI
                     EIYDKPSSKKTNKKT"
     gene            110515..111762
                     /gene="rho"
                     /locus_tag="VGB16_06115"
     CDS             110515..111762
                     /gene="rho"
                     /locus_tag="VGB16_06115"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011524029.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004386 - helicase activity [Evidence
                     IEA];
                     GO_function: GO:0008186 - ATP-dependent activity, acting
                     on RNA [Evidence IEA];
                     GO_process: GO:0006353 - DNA-templated transcription
                     termination [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="transcription termination factor Rho"
                     /protein_id="HEX9917295.1"
                     /translation="MDIVELKSKTIAELTGLAEELEIPGVSGFRKQELIFKILEAKTE
                     KNGLIFAEGVLEILPEGYGFLRSPDYNYLPGPDDIYVSPSQIKRFDLRKGDTVSGQVR
                     PPKDSEKYFALLKIEAVNFENPEVAKHKILFDNLTPLYPEKKIKLEISPNEVSMRILD
                     LLAPIGKGQRALIVSPPKAGKTILLQKIANSVTTNHPEIKLIVLLIDERPEEVTDMKR
                     SVKGEVISSTFDEPADRHVQVADMVIEKAKRLVEHQQDVVILLDSITRLARAHNAVVP
                     HSGKILSGGVDANALQRPKRFFGAARNIEEGGSLTIVATALIETGSRMDDVIFEEFKG
                     TGNMEMVLDRRLSDRRIFPAIDINRSGTRKEELLFSEEELNKVWILRRFLNEMNAVEA
                     MEFLLDRVKKTKNNKQFLDSMKE"
     gene            111865..112155
                     /locus_tag="VGB16_06120"
     CDS             111865..112155
                     /locus_tag="VGB16_06120"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEX9917296.1"
                     /translation="MQATLANVACIFAFCSCPIYWANFLQCIRAGVEFTSTPIVQAKT
                     RFGRLTISSSLSLRAEGLSLCFDFAQHHEFIEWSKDPALCFAGDIPEYRLHF"
     gene            112222..113208
                     /gene="pdxA"
                     /locus_tag="VGB16_06125"
     CDS             112222..113208
                     /gene="pdxA"
                     /locus_tag="VGB16_06125"
                     /EC_number="1.1.1.262"
                     /inference="COORDINATES: protein motif:HMM:TIGR00557.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0050570 - 4-hydroxythreonine-4-phosphate
                     dehydrogenase activity [Evidence IEA];
                     GO_process: GO:0008615 - pyridoxine biosynthetic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="4-hydroxythreonine-4-phosphate dehydrogenase
                     PdxA"
                     /protein_id="HEX9917297.1"
                     /translation="MRKPKIGITMGDPAGIGPEVLVKALKSAEINKNCIPVIFGSYEI
                     ILQAAQKYAKNLKITKLESLDQIEESKINLFDCTSINPKQIKTGKINYASAKMAIDSV
                     LACAKACRDRKIDAIVTAPLSKRGLRLAGYDFPGHTELLASLTKSKNYAMMFVGDKLK
                     VILATTHLPLSEVSRHLSIGLILKKIKLADFSLKSIFKKKKPRIGVCALNPHAGENGI
                     FGKEERQIIVPAIDLARKKKIEVLGPYPADSIFSPEISGKFDCVLAMYHDQGLIPLKL
                     SGIGNSVNLTLGLPFVRTSPDHGTALDIAGKGIANPNGMIFAIKLAIVLRRI"
     gene            113741..114391
                     /locus_tag="VGB16_06130"
     CDS             113741..114391
                     /locus_tag="VGB16_06130"
                     /inference="COORDINATES: protein motif:HMM:NF012235.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005524 - ATP binding [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="ATP-binding cassette domain-containing protein"
                     /protein_id="HEX9917298.1"
                     /translation="MLKVTGLSKIYDKDIAVLEDITFEVKRGEFVVILGPSGAGKTTI
                     FRHIWLEELPTKGEIEFEKLKSSSIRKSQIPFWRKRMGIVFQDLKLLNDRDVFENVAL
                     PLRIKGKREKEITKKTIDILNQLGLLVHRSSYPFELSAGEKQRLALARAMVAEPLIIL
                     ADEPTVHLDAESASGIISLLRDANLGGTTILMATNQRGLLGSFAHRVIGLEKGRIV"
     gene            114388..115236
                     /locus_tag="VGB16_06135"
     CDS             114388..115236
                     /locus_tag="VGB16_06135"
                     /inference="COORDINATES: protein motif:HMM:NF014717.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ABC transporter permease"
                     /protein_id="HEX9917299.1"
                     /translation="MSYRYSFREMGLSFLRQPITFLGSVITLALSLLVFNTFLLVTLS
                     LKNVTEEFKSKMGIEVYLRDDLKKEEISAFYNKLMGMPEIEEVTFKSKDEALKEMKKF
                     FKPELLEGLKTNPLPASFLIKLKKPYQGFADMEKVSSKIKEFGGVEDVEYGGEWVKKI
                     DQAIFVFLLIDIFFGLLIAILVMMIIAGNVRNMINSRVEAISTIKLLGGNRAFISRPF
                     FLQGLIEGGLAGIISLGLLYLVYRIFVSNLFKVEFLPLDLALSIVLSAILLGGVGSLL
                     ALSKHI"
     gene            115625..116785
                     /locus_tag="VGB16_06140"
     CDS             115625..116785
                     /locus_tag="VGB16_06140"
                     /inference="COORDINATES: protein motif:HMM:NF013700.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="peptidoglycan DD-metalloendopeptidase family
                     protein"
                     /protein_id="HEX9917300.1"
                     /translation="MKSKRLFVFLFLLLLFSFPQAQAQKGEAGAKKQQEELNNIRKEL
                     EYKRKKLKELKNKEGAALTELQNLQEELELMEQLIKKLDTRLGNVKIELKRENQELDS
                     IRVDLEKRRKLLAGRIRGIYKFWRFYQYEAILGSSSVADLLGRLKYLQLIAKQDNELI
                     DGFLKDKEKLEKSKKNSEAKKNELYGLKKEKEDEEKSRTLEKQKKEKVLNKIKSEKKA
                     YLQAVKELEGSARKIEAFLTKYEEERKKEESEQDLFKLLKGRLDWPVKGEVISNFGEQ
                     VHPVFKTVTFNQGIDIQANPGTEVKAVEEGKVIYSSYLRGRGEFLILQHEYGYYTIYA
                     DLGDVLVDEGEEVIKGQTIARVGELSLFSSFGLHFELRKGKKQLDPLEWLKE"
     gene            116782..117876
                     /gene="holB"
                     /locus_tag="VGB16_06145"
     CDS             116782..117876
                     /gene="holB"
                     /locus_tag="VGB16_06145"
                     /EC_number="2.7.7.7"
                     /inference="COORDINATES: protein motif:HMM:TIGR00678.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0009360 - DNA polymerase III complex
                     [Evidence IEA];
                     GO_function: GO:0003887 - DNA-directed DNA polymerase
                     activity [Evidence IEA];
                     GO_process: GO:0006260 - DNA replication [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="DNA polymerase III subunit delta'"
                     /protein_id="HEX9917301.1"
                     /translation="MTFSKVIGQDLVKQVLNRALERDKLAGSYLFFGPEGVGKWATAL
                     EVAKRLNCQKGNLGPCGNCASCKKITRLSHADITLLFPLPAVKKEEEKGKFREEFKNE
                     KIEEPYSIMCFDKPSNIAVEEIREMQRELSLKPYEAKRRVVIVYQAEKMAVAGGNSLL
                     KSLEEPPLDAVLILTSSEPSKLLPTILSRCQKIRFSPLADALIEDYLITNYKLSSQQA
                     RFCSKLSRGSLGKALSLMDSERSEIREDGVNLLRCALKEELVSSVELVGGLLRKWDRD
                     SILEMFDFLFTFLRDIYLYLEEEGDDDLLNLDLKEEIIKLAGILKEGELVEEGMGLIN
                     RTKEDCIKRNANLELSLLALVLSWRSYIRR"
     gene            117879..118727
                     /gene="ricT"
                     /locus_tag="VGB16_06150"
     CDS             117879..118727
                     /gene="ricT"
                     /locus_tag="VGB16_06150"
                     /inference="COORDINATES: protein motif:HMM:NF041131.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="regulatory iron-sulfur-containing complex
                     subunit RicT"
                     /protein_id="HEX9917302.1"
                     /translation="MSEVYLVQFKGSRKGYYYNPNYLNLKLEDYVIVQVERGEDMGRV
                     VKKSSEELILNEEKKPSNILHKATPDDIERLKANREKEEEALKECERMIGARNLEMKL
                     VDAEFQFDCNKITFYFTADKRIDFRELVKELASVYKTRIELRQIGVRDEARWIGGYGC
                     CGLKVCCSTFIKGFEPISTQLAKEQNLSLNPAKISGNCGRLLCCLLYEKDFYHEAFSS
                     YPKVGSKYQTDKEEGIVDNVNPLGGYIVVRHQDETEEKVSLLDIRKGQKKKHLPRGTK
                     QKSEVG"
     gene            118983..120890
                     /gene="metG"
                     /locus_tag="VGB16_06155"
     CDS             118983..120890
                     /gene="metG"
                     /locus_tag="VGB16_06155"
                     /EC_number="6.1.1.10"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_013274877.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0000049 - tRNA binding [Evidence IEA];
                     GO_function: GO:0004825 - methionine-tRNA ligase activity
                     [Evidence IEA];
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_process: GO:0006431 - methionyl-tRNA aminoacylation
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="methionine--tRNA ligase"
                     /protein_id="HEX9917303.1"
                     /translation="MDKRYLITTPIYYVNDQPHIGHAYTTIAADVLARFHRINQEEVF
                     FLTGTDEHGSKVAETAKRAGKTPQELCDINHKFFVQAWKNLDIAYDYFVRTTDHRHEK
                     AVWALLEKMKNSKTPDKKEVIYPGQYEGLYCVGCEKFITEKDLQNGICPEHPNMKIER
                     LSEKNYFFRLSSYLGKLKDLIGEDKIKILPEERKKETLGLIKQKIDDFSISREKVAWG
                     VSLPFDPSQKAYVWVDALPNYISAIGYGDNLEEFKKWWKEAVVLHLIGKDILKFHAIY
                     WPAMLLSVGERPPDTIFAHGYFTVDNRKMGKSLGNVIDPNFLVTSFGSDAARYLLLTQ
                     FPFGQDGDVQLSRFNEKYNSDLANDLGNLVSRVLKMIQNYCDGKIPLASTYQGIDRKL
                     IDLATAIPSTVMENVNAINLNGAIEQIMNLVKEANRYVESQSPWNLAKSGQKERLGTV
                     LYVSSEVLRIISALFYPVLPQKITGLRALLGFPKNKLIPNIKEESKWGLLKAGTEIKE
                     FESLFPRILEEKAPKEEAGVEEINLEEFSKIDLRVANVLSAEKVSGADKLLKLEIKIG
                     DQKKQIVAGIAQYYSPQDLIGRKIVVVANLKPAKIRGIESNGMLLAAKDGESLVLVTT
                     DKDIKDGAKIN"
     gene            120895..121665
                     /locus_tag="VGB16_06160"
     CDS             120895..121665
                     /locus_tag="VGB16_06160"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_013780161.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016788 - hydrolase activity, acting on
                     ester bonds [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="TatD family hydrolase"
                     /protein_id="HEX9917304.1"
                     /translation="MIDTHAHLDSPQFDKDREKVIQEAKSCGVERILNVGVDQESSQK
                     SVDLAQRYPQIYATVGFHPHDAKKFNQSSLEELRRLAQHQKVVALGEMGLDFYRNLSP
                     AEIQKSVFISQLELAGELDMPVVVHIRNAYPQSLDILEQRKSVKGVLHCFEGDIQEAK
                     RGLDLGFYISFNGRITYHNPSLIDLVKKLPLDRILVETDSPYLPPHPHKGQRNQPAWV
                     ELVIREIAQIKGKLVFEELEKITTINACNLFGWKQDEE"
     gene            121655..122488
                     /gene="rsmA"
                     /locus_tag="VGB16_06165"
     CDS             121655..122488
                     /gene="rsmA"
                     /locus_tag="VGB16_06165"
                     /EC_number="2.1.1.182"
                     /inference="COORDINATES: protein motif:HMM:TIGR00755.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0000179 - rRNA
                     (adenine-N6,N6-)-dimethyltransferase activity [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="16S rRNA
                     (adenine(1518)-N(6)/adenine(1519)-N(6))-
                     dimethyltransferase RsmA"
                     /protein_id="HEX9917305.1"
                     /translation="MRSKTKENQTSKPRKSLGQNFLINKWAALSIVKSLNLSDSDIVL
                     EIGAGKGALTGYLLERCKKVVAVEIDKRWCEYLEDKFRGFENLIILNQDILKLDLKKY
                     AQGKKMKIVGNIPYHLTAPVISYLIENKNLISQALLTVQREVAKRICAKPGSGDWSLL
                     SIFTQLHACTKLLFVLEPNWFFPQPKVHSAVVELVFLNGLAVGVENIDYFFKIAKMIF
                     QQKRKMIINSLTSGLDLSKSEIKEKFEDIGIDPSLRPQELGLDKLAIISSVMIGSTNK
                     P"
     gene            122498..123220
                     /locus_tag="VGB16_06170"
     CDS             122498..123220
                     /locus_tag="VGB16_06170"
                     /EC_number="2.6.99.2"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_014396362.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0033856 - pyridoxine 5'-phosphate synthase
                     activity [Evidence IEA];
                     GO_process: GO:0008615 - pyridoxine biosynthetic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="pyridoxine 5'-phosphate synthase"
                     /protein_id="HEX9917306.1"
                     /translation="MPKLSVNVDHVATLREARKVDQPDPVVAAIMAELAGADGITVHL
                     RKDRRHINDRDLGILREVIKSSLNLEMAATEEMVERAIEIKPDMVTLVPEKKEELTTE
                     GGLDLQANFENLSQRVSKLQINEMFVSLFVNPDMESIKQAAKLRADFVEINTDAYSRA
                     KKLSQKIEELERIERIVGLAYKLGLRINAGHGLNYTNISDLVMIEHIEEFSIGHAIVA
                     RAVLVGFERATREMLDLVKKRG"
     gene            123277..124248
                     /locus_tag="VGB16_06175"
     CDS             123277..124248
                     /locus_tag="VGB16_06175"
                     /inference="COORDINATES: protein motif:HMM:NF012568.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_process: GO:0008299 - isoprenoid biosynthetic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="polyprenyl synthetase family protein"
                     /protein_id="HEX9917307.1"
                     /translation="MKLDKIFAPVKKQLVSFDKTLEEALSSDSSLIFEVVKHILDGKG
                     KRLRPSLVFLCWGMCGGKSKNATKVAVAIDLIHNATLLHDDVIDQSPTRRGKASVNCK
                     WNNLVSILMGDYLLSKAFKIMNQTESLELMNDMSRATENLTIGELRQVQEARNYQLNE
                     KTYLKMIEDKTASLFSASCMGGGITAGAKEEQRKHLSEYGRNLGITFQITDDVLDFIG
                     ESKKTGKGLGNDLKEGKVTLPLIYTLRQAEGATKRRIIRLLEKDFNYQDFVKILSLVR
                     EYGGIDYAKWRAEIFGQKALSHLAGFKNSPYKQSLMDMADFAVNRDR"
     gene            124559..125854
                     /gene="eno"
                     /locus_tag="VGB16_06180"
     CDS             124559..125854
                     /gene="eno"
                     /locus_tag="VGB16_06180"
                     /EC_number="4.2.1.11"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_013298465.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004634 - phosphopyruvate hydratase
                     activity [Evidence IEA];
                     GO_process: GO:0006096 - glycolytic process [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="phosphopyruvate hydratase"
                     /protein_id="HEX9917308.1"
                     /translation="MPQIVSCFAREILDSRGNPTVEVDVILEGGAWGRAAVPSGASTG
                     EHEALELRDGDKTRYLGKGVLKAVNNVNQIIAAELVKQGLDATQQVGIDKFLIALDGT
                     EDKSKLGANAILGVSLAVCKAAAYYLGLPLYQYLGGVNAKILPIPQMNIINGGKHADN
                     NVDLQEFMIMPIGASSFREALRMGAETFHNLKSVLKEKNYNTAVGDEGGFAPDLKSNE
                     EAIQVILKGIEKAGYKPGEDIYIALDPAASEFFDRNSKTYNLKAENKKFSSSEMIRYY
                     ESLIKKYPIISIEDGLAEDDWEGWVEFTKKLGDKIQLVGDDLYVTNIKRLAKGIELGA
                     TNSILIKLNQIGTLTETLDAIAMAKQAGFTAVVSHRSGETEDTTISDVVVGANCGQIK
                     SGSTSRTDRICKYNQLLRIEEELGDQAQFLGKKAFYNLK"
     gene            125851..126222
                     /locus_tag="VGB16_06185"
     CDS             125851..126222
                     /locus_tag="VGB16_06185"
                     /inference="COORDINATES: protein motif:HMM:NF016839.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_process: GO:0051301 - cell division [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="septum formation initiator family protein"
                     /protein_id="HEX9917309.1"
                     /translation="MKKGKLIAILDNRFFARVKKEATKKKGKLRAILFLMALLLLGYL
                     YMAGDYGFVRIFSLWKEKKSLVMEIKKIEAKSIDLKVEKQKLTSDHDYIERIARERYG
                     MVKKGEKLYKFVQKEPKDLSR"
     gene            126219..127007
                     /gene="recO"
                     /locus_tag="VGB16_06190"
     CDS             126219..127007
                     /gene="recO"
                     /locus_tag="VGB16_06190"
                     /inference="COORDINATES: protein motif:HMM:TIGR00613.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003677 - DNA binding [Evidence IEA];
                     GO_process: GO:0006281 - DNA repair [Evidence IEA];
                     GO_process: GO:0006310 - DNA recombination [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="DNA repair protein RecO"
                     /protein_id="HEX9917310.1"
                     /translation="MSICKASALVLKSINLRESSRIVTLYSKEFGKMKVVAKGMLLPQ
                     SRFGGSLENFSEINIVFYKRENTELYILSQVDLVNPFHKLCLDLDRFAIASTGMELLD
                     KLVSWEESSSKIFDLSLDFLSQIESTGKSNLKKILLGYVLKLVTILGYKPELEDCVAC
                     DKKIKDRFIFFNPERGGLVCPSCLIDDGFYIKFTRDSIEKASQLRRLSLKSAGKIKMS
                     ESQSDEVLCGILDFLDFHVGRGKDLKSMEFFKSINSGGISESEG"
     gene            127012..128298
                     /locus_tag="VGB16_06195"
     CDS             127012..128298
                     /locus_tag="VGB16_06195"
                     /EC_number="6.1.1.14"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_015710570.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0000166 - nucleotide binding [Evidence
                     IEA];
                     GO_function: GO:0004820 - glycine-tRNA ligase activity
                     [Evidence IEA];
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_process: GO:0006426 - glycyl-tRNA aminoacylation
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="glycine--tRNA ligase"
                     /protein_id="HEX9917311.1"
                     /translation="MEKIVSLCKRRGFVFQSSEIYGGINSCWDYGPLGVELKRNIKDY
                     WWRCMTQLRNDVVGIDASIIMHPRVWETSGHVAGFVDPLVDCRKCKQRFKADQLKENK
                     CPECGGELTSARQFNLMFKTHLGPVEDDASIAYLRPETAQGIYVNFLNVLGPSRQKIP
                     FGIAQIGKAFRNEISPGNFVFRSREFEQMEMQYFVHPKEDVKWLEYWKNERMNWYLGL
                     GVREENLKLHQHTKKELAFYAKDAFDIEYNFPFGWQEIEGIHNRTDFDLSRHSQATGK
                     DLSYFDDQTKEKYIPYIIETSAGVDRSLLVFLVDAYYEEEVKGEKRVALRLDPKIAPI
                     KAAILPLVKRDNMPEIAQDIYKKLKPHFNVFYDEGGAVGRRYRRQDEIGTPFCITIDS
                     QTLQDNTVTLRERDSMEQVRVKIDDLREELERRIWG"
     gene            128327..128737
                     /locus_tag="VGB16_06200"
     CDS             128327..128737
                     /locus_tag="VGB16_06200"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEX9917312.1"
                     /translation="MKICPRCGKELEDNAIVCKYCSLGFGFGILWAGLAIMVGVLGIL
                     GPFIASTLLRAGPGLIEIIALAVISVILIVAGILVLKKRKLGLYIVFVLIPIDIISSF
                     IALLTGGDFVWFLISFILGSLWLWYFYRRRYMFK"
     gene            128867..129568
                     /locus_tag="VGB16_06205"
     CDS             128867..129568
                     /locus_tag="VGB16_06205"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_017889084.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="SagB/ThcOx family dehydrogenase"
                     /protein_id="HEX9917313.1"
                     /translation="MKKKHSIIFLVVILAIFLFIFPVTNGQNEIPLPNPTLKGQKSFE
                     EVLSTRRSRRSFKEGAIALKELSQILWASQGITSKDGKRTAPSAGALYPLEIYVVVGE
                     VEGLEPGIYHYDAPNHKLIRVIDKDKRSELNLATGGGRRNIENTPVSIIITAIYGRTT
                     KKYDQRGIRYVHIEAGAVCQNIYLQVESLNLSTFVMGAFDDAEVKKVLALNQNGSVLG
                     GEVEPILIMPIGKRI"
     gene            129826..131223
                     /gene="tilS"
                     /locus_tag="VGB16_06210"
     CDS             129826..131223
                     /gene="tilS"
                     /locus_tag="VGB16_06210"
                     /EC_number="6.3.4.19"
                     /inference="COORDINATES: protein motif:HMM:TIGR02432.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016879 - ligase activity, forming
                     carbon-nitrogen bonds [Evidence IEA];
                     GO_process: GO:0006400 - tRNA modification [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="tRNA lysidine(34) synthetase TilS"
                     /protein_id="HEX9917314.1"
                     /translation="MEVKNSFVKKVEKTIQHYKMLQQGDKVLAAVSGGPDSVALLHTL
                     NLLKDIFKIKLIVGHINHQLRASESDKDEKFVKALSEKLNLKCFSKSIEVKKTAQKKK
                     ISIEDCARQMRYSALESIANKTKADKVALGHNLDDQAETILIRLIRGSGPLGLCGIPA
                     VRGKLIRPLIQTKREEIENFLKDNLWEWRVDWSNFNMDYLRNRVRLKLLSLIKKEFNP
                     NIVEVLARTAQLLSEEKDYLTNQIEKAFRDAVSSQDSEKIVLDLKKLIGYDIYLKTNV
                     IRLAFNRLSGDLDFPSFSIVQRATGLLEERKAGKRVELGKNIWVEVSSDALIVYKSRK
                     PAKAHWVKLPGKLEIDNLKLKSEVIQRPKLPKKIKSNSPWIAYLDWDKLSSPFGLRHR
                     KNKDWFIPLGLGGSKTLADFLIDLKVPRMERDEIFLLTSKNRIAWVIGQRIDDRFKVN
                     SNTKEVLKIEAFLEN"
     gene            131234..131758
                     /gene="hpt"
                     /locus_tag="VGB16_06215"
     CDS             131234..131758
                     /gene="hpt"
                     /locus_tag="VGB16_06215"
                     /EC_number="2.4.2.8"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_012547340.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_process: GO:0006166 - purine ribonucleoside salvage
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="hypoxanthine phosphoribosyltransferase"
                     /protein_id="HEX9917315.1"
                     /translation="MEKIKVKKIISKSQIQKRVKKLSQTISRDYIKRDLILVGILKGS
                     FVFLADLTRNLKIHHQVDFISVTSYGSSTRASGIVRILKDLSVNIQNRDVLIVEDIVD
                     SGLTLNYIIKNFKTRQPKSLKVITLLNKKKKREVSIPLKYIGFDIPDEFVVGYGLDLN
                     ERFRNLPFIAKIEK"
     gene            131771..133744
                     /gene="ftsH"
                     /locus_tag="VGB16_06220"
     CDS             131771..133744
                     /gene="ftsH"
                     /locus_tag="VGB16_06220"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_020706902.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004222 - metalloendopeptidase activity
                     [Evidence IEA];
                     GO_function: GO:0016887 - ATP hydrolysis activity
                     [Evidence IEA];
                     GO_process: GO:0006508 - proteolysis [Evidence IEA];
                     GO_process: GO:0051301 - cell division [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="ATP-dependent zinc metalloprotease FtsH"
                     /protein_id="HEX9917316.1"
                     /translation="MQKDDKKIRLDRKEDDRRSPRDQEKNQFQWKRSGRTLIFWIALL
                     VLSILLFQYYSRGGKDIIDISYSEFQQELLQSNIKSVLVIEKNVEGEFVKEISKSDRG
                     RTQSYPHFRTYLPVESQELISQMQQKGVQIEAQAASVNWSGMLLTSLPWILLLAFFWF
                     FLFRQMQGSSKGIFSFGKSRARLLTGDRPKVTFEDVAGADEAKQELQEIIEFLKEPGK
                     FQKLGGKIPKGALLLGAPGTGKTLLAKAVAGEAEVPFFSMSGSDFVEMFVGVGASRVR
                     DLFEQGKKNAPCIIFIDEIDAVGRHRGAGLGGGHDEREQTLNQLLVEMDGFESNEGVI
                     LLAATNRPDVLDPALLRPGRFDRQIIVDIPDVKGREGILKVHTKKIKLGEGVDLSILA
                     RGTPGLCGADLANMVNEAALLAARHNRDGVTIQDFEDARDKVMMGVERRSLVISEEEK
                     KTTAYHESGHALVAKLIPGSDPVHKMTIIPRGLALGLTHYLPVDERHTHSKEYLENKL
                     AQLLGGRVAEKLVFNQYTTGAGNDLERATELARKMVCEWGMSEKLGPLTFGKKEEEIF
                     LGREIAKHRDYSEETAKQIDAEVRKIVEEAEAKATNLLQGNQEKLHTLAKALVEKEIL
                     DGEQIDRILKGEKLEPGKSNNENENKKDKPKEN"
     gene            133710..134339
                     /gene="folE"
                     /locus_tag="VGB16_06225"
     CDS             133710..134339
                     /gene="folE"
                     /locus_tag="VGB16_06225"
                     /EC_number="3.5.4.16"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_012032569.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003934 - GTP cyclohydrolase I activity
                     [Evidence IEA];
                     GO_process: GO:0042559 - pteridine-containing compound
                     biosynthetic process [Evidence IEA];
                     GO_process: GO:0046654 - tetrahydrofolate biosynthetic
                     process [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="GTP cyclohydrolase I FolE"
                     /protein_id="HEX9917317.1"
                     /translation="MKTRKISRRKIEEGIRLALEGAGENPQREGLKDTPRRVADLYEE
                     IFAGLGIDPQKELKIYNTPNQDEMIIARDIPFYSMCEHHLLPFFGKVHIAYIPQKNKV
                     TGFSHLAQVVEIIAKRPQLQERLSSQIADVLMRGLKAKGVLVIIEAEQLCLTMRGLKK
                     QGVSTITSAIRGVMKKSNIRAEALGLIKSELPRPKSGHPKHLLPLEGED"
     gene            134581..135360
                     /gene="folP"
                     /locus_tag="VGB16_06230"
     CDS             134581..135360
                     /gene="folP"
                     /locus_tag="VGB16_06230"
                     /EC_number="2.5.1.15"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_012545277.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004156 - dihydropteroate synthase
                     activity [Evidence IEA];
                     GO_process: GO:0009396 - folic acid-containing compound
                     biosynthetic process [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="dihydropteroate synthase"
                     /protein_id="HEX9917318.1"
                     /translation="MGILNVTTDSFSDGGKFFKFEDAKRQALKMAEEGADIIDIGGES
                     TRPGSEAVPLEEELERVIPLIEDLVKSTSIPISIDTYKSEVAQRALDAGAEIINDISA
                     LRFDQNMADMAAGYDAGIVLMHIQGTPKNMQENPHYDNLIEEIKTYLKESIKLAEQAG
                     VKKDKIVIDPGIGFGKKVEHNLNILRNLKEFGELGKPIMVGTSRKSFIGKLLDLPVEQ
                     RLEGSLAGLAVSIMNGANLVRVHDVKESLRVVRLVDTIIRN"
     gene            135388..136152
                     /gene="cdaA"
                     /locus_tag="VGB16_06235"
     CDS             135388..136152
                     /gene="cdaA"
                     /locus_tag="VGB16_06235"
                     /EC_number="2.7.7.85"
                     /inference="COORDINATES: protein motif:HMM:TIGR00159.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004016 - adenylate cyclase activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="diadenylate cyclase CdaA"
                     /protein_id="HEX9917319.1"
                     /translation="MELFQVGFIKFGIVDLIDVAIVWFLFYRLLILMKGTRSVQIVIG
                     LFLVILVSFIAFWFQLEGLKWLISNIATVGIIVLVIVFQPELRGALAQIGHNKFIRMF
                     FRYEKEKTLDEVVRGAIKLAELRYGALIVLERDIGLKDFAETGKELGAQVSSELLVTL
                     FTPYTPLHDGAAIISGENIVAVACTLPLSQNPQYIKLYGMRHRAAVGITEESDAVVVA
                     VSEETGNISVAFGGRLQKNIDKSVLKDMLLGLMRES"
     gene            136160..137155
                     /gene="obgE"
                     /locus_tag="VGB16_06240"
     CDS             136160..137155
                     /gene="obgE"
                     /locus_tag="VGB16_06240"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_012545625.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003924 - GTPase activity [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="GTPase ObgE"
                     /protein_id="HEX9917320.1"
                     /translation="MFIDYVEILAQAGDGGNGCVSFRREKYVPKGGPDGGDGGDGGNV
                     IIKVNPNLSTLIDLRYRKVYKAQTGGNGEGGNRSGIRGKDIIINVPGGTIIKDLQENK
                     ILADLTSGDDSIIIAHGGKGGKGNSHFKSSTNRTPRKAEPGQKGERKKLSLELKLLAD
                     VGIVGPPNSGKSTLLAKVSQARPKIADYPFTTLSPNLGIVRISDSKSFVMADIPGLIE
                     GAHQGKGLGFDFLKHIQRTRILLYLIDITSPDISGEFVKIRKELKLFDKKLFDRPSVL
                     ALNKIDLLFPEQRKKIKMAKRGIPVFKISAANGEGVKKLANFLSLKLTLLVEGEN"
     gene            137152..138246
                     /gene="dprA"
                     /locus_tag="VGB16_06245"
     CDS             137152..138246
                     /gene="dprA"
                     /locus_tag="VGB16_06245"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_014559603.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_process: GO:0009294 - DNA-mediated transformation
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="DNA-processing protein DprA"
                     /protein_id="HEX9917321.1"
                     /translation="MSQDVLSYLALYSVPEVGTQRIISLVRKFGSAKEALGASLKELT
                     RVEDIGDKIAHNIKHQVNWKLAQEQFSLAEKKKFKILTFFDEEYPKSLKNIYDPPPLI
                     FFRGEIRSEDQKAIAVVGSRKASSYGRMVTESLVEELASRGITIVSGLARGIDSISHQ
                     AALKAGGRTLAVLGSGLDIIYPPENRKLADGIAENGAVISEFFLGTKPEATNFPQRNR
                     VISGLCLGIVIVEAGPKSGALLTARHALEQNREVFAVPGDIRSQGSKGTNELIKQGAK
                     LVSSVEDILVELNELTNASAKKERIERVEQDLPKEEKDIFKLLSIEPYHIDKISKELG
                     LRSSEALSFLLSLELKGLVKQLPGKVFARA"
     gene            138260..138526
                     /locus_tag="VGB16_06250"
     CDS             138260..138526
                     /locus_tag="VGB16_06250"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011026008.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_process: GO:0009401 - phosphoenolpyruvate-dependent
                     sugar phosphotransferase system [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="HPr family phosphocarrier protein"
                     /protein_id="HEX9917322.1"
                     /translation="MIERNVEIINKLGLHARPAAIFVQKASKFKSEITLEKEGQKVNG
                     KSIMGVMMLAAEKGSTLIIRAEGEDENEAVAALVEVVLSKFGEE"
     gene            138529..>138818
                     /locus_tag="VGB16_06255"
     CDS             138529..>138818
                     /locus_tag="VGB16_06255"
                     /inference="COORDINATES: protein motif:HMM:NF017346.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_process: GO:0009401 - phosphoenolpyruvate-dependent
                     sugar phosphotransferase system [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="phosphoenolpyruvate-utilizing N-terminal
                     domain-containing protein"
                     /protein_id="HEX9917323.1"
                     /translation="MEKQAQKNILLKGVSASPGIVIGETFLFDRKIVEAKEQELKEEE
                     IGKELLKFKKALAETKREMINLRDKVERRIDPDHAKIFDAQIMILEDQWINQ"
BASE COUNT        41919 a        25938 c        33225 g        37736 t
ORIGIN      
        1 cccgtttcgc ccgtctcgcc gatggctcgc cgaggcgggc cgaatcgagg cgagggcaag
       61 gggagattca ttcaacgtaa attaatgcgt ttgcattagt cgctgcgaaa gagaaaaatg
      121 caaatgtatt tgttgggttt aatcagataa gggccaaaat ggtctaaaat cattcattta
      181 aacgcagggg cattatcaag cagagatact cctctccctt ctcttttgaa gctgaatgga
      241 ccatgcctgc ggagactgga gtagccagct caaataccac ctcatcccca tccacctgtt
      301 ttaagatatc caggatgtag ttagcattat accctatctc cataatctca ttcttgtagt
      361 tacagggtaa ggtctcaatt gcttcccctc ctacttccgc attggtggcg gaaagctcca
      421 aactgtcttt tttcagggag aattttatct ggtgggttag ggtgttggat agaaccgaaa
      481 ccctcctcac caaactcgcg agtaattcct tattcacaat caacttctta tcattgtcct
      541 taggtataac ctgctcatag tccggatagg gaccttcaat caaacgggag gacaaaacaa
      601 catcccccag attgaaaatg atattatttt ccccgaatat cacccctaat tcctcgtcgc
      661 cctgatatag tttcaccaat aaatttaggg ctttgggtgg aattattatg tccttgtgca
      721 atccttttag cttggtattt tgcagtgcta ctttagctaa gcggtgccca tctgttgccg
      781 ccatacacat tacctctcca ctggtttgcc agagtatacc acttagggct ggcctggtct
      841 cgtccattga agccgcaaac ccaaccttct ttatcattcg aactaatgtc tctccgggta
      901 tatttatctc tttagccacc tggatgcttg ggatcttggg gtattcttcg ctggagctgc
      961 cagatatctt ataactccct ttatctatcc tcatctccag acgttcatct gtgatattaa
     1021 tctcaatttc tgtttccggg gcttctcgga ttatatctgt aaaggtgcga gcaggtataa
     1081 ccaggcttcc ttttttagtt gactttattg gaatggaggt ggttatggaa acatccaaat
     1141 ccgtggcgga gatttttagt tttgtctctt caaattctaa caaaaaatta gaaagtatgg
     1201 ggagtggtgt ttttccaggt actgcattaa gcgttttttg tatactgtca aataatttgc
     1261 tttttaggat tgagaatttc atttatcctc cctaagggtt tattattaga aagaagtatt
     1321 ataaatttta atactaatca ttgtgttgat tgtgtgaata taaccataaa ggtaaaaaga
     1381 cgcaacaagt taaattggat acccaatgtt aagaaggggt gggtttttgg ttgataaaga
     1441 ccaacctatg aacacaagac aaatatacac caattataaa cagacctgcc aacactattt
     1501 aaacatacaa agagcttatt agttcatcta tcttacgctt gaattcagga ttagttttta
     1561 ggttttcagt tatctgagta caagaatgaa taacggttga atggtcccga ccaccaaact
     1621 taagtccgat agttttcaaa gaggagttgg tcaggttacg agctaaatac atcgctactt
     1681 gccgggctgt caccacctct tgggttttct ttttagaaca taaaacatcc tcgggtatat
     1741 tatagttttg cgcgaccttt ttttgaatct gctcaatagt gattaacttc acaccattct
     1801 ttatggaatc ttttaaaacc tcctgggcta aatccaccgt gatctctttg ctatttaggg
     1861 aggcataagc caaaagccga ataagcgaac cctccaactc gcgaatattg gaagtgattt
     1921 tatcggctat gaaggtaaca acctctggtg ggaccgagcc actgtccgat tcaatcttct
     1981 tgttgagaat agcgattctg gtctccaagt caggtggctg gatatcagtt actaatcccc
     2041 actgaaatct cgaaaggagc ctctcctcca aacccttaat ctctttgggc gaccggtcag
     2101 aagttaaaac tatctgcttt ccgctctgat acagagcgtt aaaggtgtgg aagaattgga
     2161 cctgggtgct ttccttgccg gcgaaaaact ggatgtcatc caggaggaga acatcgaccg
     2221 aacgataacg attgacgaac tcctgcatag tgctggtgct aatggagttg atgaaatcgt
     2281 tagtgaaggt ctcgctggta acataaagta ccttaatatt cttgtatccc tccagggcaa
     2341 gatgcccaat agcttgggcc aagtgggttt ttcctaaccc tactcctcca taaatataga
     2401 gcgggttgaa ctttgtcttg cctggagctt cagccacagc catggcagct gcgtgggcaa
     2461 agcggttact ttcccccacc acgaaagtat caaaagtata acgagaatta agttggggtg
     2521 agatagccgg atgatagcgg gttttttggt caagtgaggc tttgttattc tggcttggct
     2581 cgtagcttct ctgggctccg gtgcgtatgc agaatatcgg attcaattcc ttttgggtaa
     2641 ccgacttaat tgcttctcta atcagagaag gataatgctc ctctatccac tccgccacaa
     2701 agcgattggg cacttctatt cttagctcgt catcggaaaa gtcaagccct ttggtgggcc
     2761 ttagccaggt gaagaagctt tgttttttta ctttatgctg aagaaaacct aagcaatctt
     2821 cccagatctg aggaccgttt ttcaaagata aagtatccac agttgttgat aaaaattaat
     2881 atgttgaagg tatttcagtt aagcggttta agtaagacta aaaagaaaga tttccacatt
     2941 ctcgcaactc caatccagta gaatcaatca tattcgacaa taggttgcaa gcgcaaaatt
     3001 ttctccacat cttattaaca cagttgaact tagctatcgt aacgacatta taacttttat
     3061 ggtttctttg tcaagagatt ttctaaaaaa actccaacaa aatttaaagt gataccctat
     3121 tttttgttag agaaacaaat ttcaagctca aagagagggg aagcagttca agttgaaaaa
     3181 tagtggctga attaaagagt gtctgcttta gcgttctgct ataagcaact agaaatcaaa
     3241 agaagtaaaa gaaggatatt ttgcttgact aaaagcatat tgaaattata ttgcaaaatc
     3301 aaccacagaa aagaggtatg cgaaatgaag agaacctttc aaccctcaag aattaagaga
     3361 ttaagagatc acggatttct taaaagaatg tcgaccaaag gaggaaggaa agtattgcaa
     3421 agaaggagag gcaagaaaag aaaaaggttg acggtcaagc cgggcagatg aaagagtctt
     3481 tacctaaggt aatcaagaag aaagaggaaa tcgaggagac tatccagcag ggagaaaaaa
     3541 gaaaagggaa gtatctgctc gggtttgtgc gcccttattc ccaagaaaat tttagcttct
     3601 gtatcttaat caatagaaag ataaaaaaag cagttgagcg aaatcggata aaaaggcttt
     3661 taagggagat agttcgcaag aataaggaaa ttttcagaac caaagggagg attgctctgc
     3721 tttacatttc cagatcagcc gagacgagct attctgaact ggagaatgac cttaagggaa
     3781 tgttttatga gaatgatcga taagtttttg caagcggtct taatagttat catacgattc
     3841 tatcgataca gtctaagtta tttctttcca agcagttgta gatttgaacc gagttgttcc
     3901 gaatatgcca tttctgcttt gagaagttat ggagtagtaa aagggtcttg gctaacgata
     3961 aaaagattat ccaggtgcca tccctttcat ccaggcggat atgaccccat aaaataggga
     4021 gagatttatg gaaaagaaaa accttttagc cttagttcta atcagcctac tcactatggg
     4081 ttatttcatc tatacgggct ccattacccc taaagagccg agccgtcctt attctgcgaa
     4141 aggggggatt gaaattgata cctcttcagt aggaacccag aaatcacctg agttaaaaac
     4201 ggagcgcggg aagtttgctg ttcaggatac aaccacaccg gaaaaagtag taaaggtgga
     4261 aaccgacctt tacagggcac atttctcaag taggggcgcg acgttgaaga gtttcttgct
     4321 taagcgatat aaatattcag agaatggaaa ccaaattgag cttattcctc aagatagtcc
     4381 gggaagtttg ggcatactgt tttcggattc gacactgttt tctttccaag ttgacttaga
     4441 tagtctaatt cttaatgacg taacacaaag gggcacaata actttcaatg gtcatgcaaa
     4501 agatggcaca acaataacaa aaaaatacac cttctataat aacaaatatc atttcaactt
     4561 agagctcgaa atctcagagg atggacaggc tgaatcgaga agagattatg ctttggcttg
     4621 gtcatccggg cttgcctcaa ccgagaaaga tctaaacgag gacctgcgct attttgaggc
     4681 ttattctatg atggggcagc aacttcgcag tagtaagaaa ttcaaaaagg aaagtggtac
     4741 agaaatggat gtgctggagg aatctaattc tggccagacc gactgggtag caaccaaaac
     4801 taaatatttc ttggcttcaa ttgttcccct atccaagaaa ggatcaggct tttcagttta
     4861 cgggaaaagg tggtttagcg gtcaagaggg tcaaagactt gagcacaaac agatagctgt
     4921 cttcttggag atgcccttcg ccagggatag tatagcaata gacagtttca tggtgtacct
     4981 tggtccactg gattacaata aattaaggag ctataaaata ggacttgaga acacggttaa
     5041 cttagggtgg aaaataatca agccgttttc cattgccatt ctttggattt tcgtgaactt
     5101 gcacaaatta attcctaatt atggcttggt gataatcata tttaccatct taatgaagat
     5161 tgtgctccat cccctaacgc ataagagcac aaaatcaatg agtaagatgc aagagctcca
     5221 gcccaggata acagaactaa aggagaagca caaggacgac actcaaagga tgaaccagga
     5281 gatgatgaag ctttataagg agcaaggagt taaccctctg ggcggatgcc tgcctttatt
     5341 gctccagatg cccttgtttt atggtctgtt cgtgatcttc caaagcacca ttgagctgag
     5401 aggagcagaa tttgcttggt ggctgaagga tttgtcgcaa aaggacccct actttatcct
     5461 tcctttaatt atgtcagtag ctatgttctg gcagcagaag ataaccataa gagatcccaa
     5521 gcaaaagatg atggtctatc ttatgcccat actattcttc ttccttttca aaggcttccc
     5581 cgcaggcttg accctttatt ggacactctt caatatcctc tctttggctg aacagtatta
     5641 ccttaaagcc aaacatgcca gcgaagcagt ttccgcatga aaaaaaaaat ttcaaagccg
     5701 gcagtgatac tattgccgcc atttccactc ctccaggaga aggcggaata ggcattgttc
     5761 ggctctccgg caacgactcc ttcaaaatcg cagacaagct cttcgcaggc aagaccctgc
     5821 ccagccagat gaaaagccat actgtctgtt ttggaaaact gatggatgcc acaagcagcg
     5881 aattgttaga tgaggtgctg gtgaccgtat tgcgttcccc taatagctac acttgtgagg
     5941 atatggtgga gataagttgt cacggaggcg gtttggttct atccaaaata ttagagcaaa
     6001 cgctcagatg cggagcaaga ttagccaatc ccggagagtt caccctgaga gcttttttaa
     6061 atggaagaat ggacttggct caggctgaag ccgtggttga tataatccgg gcaaagacag
     6121 attcaagttt gaaagttgcc ctgcagaatt tggaaggtaa gctctcaaaa aaactaaatc
     6181 attgccgtga aagactaatc gatatattgg cagagataga gttaggagtg gatttcattg
     6241 aggaagatgt tgaggaatta gatagaaaaa agataaccaa gaagataacc gaactggaaa
     6301 tagatataaa gaatctcttg gacagctatg ttgaaggccg cattttaaga gagggattga
     6361 atgttgttat tgtgggaagc cccaatgttg gcaaatcaag tcttttgaat gcgttcttag
     6421 ggaaagacag ggcaattgta actccgattc caggaacgac cagagacaca atagaagaat
     6481 tcgttagcat aaaagggatt ccagtaagac tggttgatac tgccgggttc catatctcta
     6541 agaacaaaat agaattagag gggataagaa gggcgaaagt tgagatagat aaggcagacc
     6601 ttgtggtttg ggttgttgat ttgtcaaaga aacttagtga aaatgagaga aagttagaag
     6661 aattaatttc caagtataag tatgtcgtgg cactaaataa gatcgatata gcttcaagtc
     6721 gaactgtaaa gaaaatgctg gcagaattca aaaaaagaga tgttatgaga atatcggcat
     6781 tgcaatccat tggtattaaa aaccttagag atttcattat ttcttccttt ataggcaaga
     6841 ctaaatcaga gaacaacctg attatctcca atttaagaca taagcaatta ttagaaaaga
     6901 gcttagagaa tttgaaaagg gcaaaagaaa gtcttgaaaa gggaatgagt cctgaattcg
     6961 ttgctcttga tctaagaatg gcgcttgact ctattggtga gatagttgga aaagtggtta
     7021 cagatgatat cttaaatcga atattctcca aattttgtgt aggtaagtga agcctccccg
     7081 tgataaatca cggagcttcc caaatatgaa aggccctcgg cttccctcgg cttcgccgag
     7141 aggcgagcga gactttctgg tagaggcgag taattataat ataagatgtt acaatgataa
     7201 tctataaaga agaatatgaa gtgattgtgg tcggtgctgg ccatgctggt tgtgaggctg
     7261 cgcttgccac atcccgtatg ggtcacaaaa ccctacttct gacaatgaat aaagaggcga
     7321 ttgctcaaat gtcttgcaat ccggcagttg gtggtcaggc aaaagggcat ttagtcagag
     7381 agatagatgc attaggtgga gagattgggt ttaatacaga taaggcagct gttcaactaa
     7441 aggttctaaa caagagaaag ggtcctgcag tttggtcaac cagggcacag tgcgatcgca
     7501 cagcatatcg tgttgctatg cggcaggtgc ttgaaagtca gccgaatctc tgggtaaaac
     7561 aaggaaatat aattgagata ttggctgaca agaacaaagc aaaaggagta ttgaccgaga
     7621 ccggaaccgc ctatttaggc aaaatcctta ttctctgtgt ggggacattt ttaaacggct
     7681 tgatccatat aggacttacg aaatttgaag ccggaaggat gggcgaattt ccagcaagag
     7741 ggataactga aaatctgatt aaactcggtt ttgaagcggg aagacttaaa accggtactc
     7801 caccaagact agatggaaag acaattgatt ttgaaaagac ggaaatccaa tggggagatg
     7861 aaaatccaac cccattttct ctgagaacta acaaattatc cttcaaggac ttaccctgtt
     7921 ttttgactta tacaaattcg gatacacaca aattcatatt gaaaaactta gatcgctcac
     7981 ctctttacag cgggattata aagggcatcg gaccacgcta ttgtccctca attgaagata
     8041 aagttgtaag attcaaagag aagccgagac atcagctttt tcttgaacca gaaggttata
     8101 acacaaatga atactatcta aatgggtttg ccacaagcct gcctgaagat gtccagcttt
     8161 ccgctttaag aacagtggca ggcttggaaa aagtggagat gaccaggccc ggatatgcga
     8221 ttgagtatga tttcttccct ccgattcaac tgaagaacag tttggaaaca aagttgattg
     8281 aaaacctttt ctttgccggg caactaaacg ggacttccgg atatgaggag gcagcagcac
     8341 agggcatagt ggcaggcata aatgccggtc tgaaattaga agaagaagag gcatttgtgt
     8401 tggaccgaag tcaggcttat attggtgtcc tgatagatga tttgataacc aaagggacaa
     8461 gcgagcctta ccgtatgttc acctcccggg ctgagtatcg actccttcta agagaggata
     8521 atgcggacca gaggttaaca gaatatgggc ataaattcga cttaatagat aaggaaatat
     8581 actctcgagt taagatcaag atgaagaata tagaggagga aagaggaagg ctcaaaagga
     8641 cttacactaa actctccttt tttaatcttg agtccgacaa taacaagaga atatctttag
     8701 ctgatgcctt aaagattccg ggtataactt acgagaattt ggctaaggtg gacaaggcca
     8761 gccagagcat ttctcctcat gtagctttgc aagtagagtt ggagactaaa tatgaggggt
     8821 atatcaaaag acaggaagaa gaggtggcga aattcagaag gcttgagaat atggttattc
     8881 cacaagattt tgattatcaa ggacttagtg gtttcaagaa agaggctttg gaaaagttaa
     8941 ataaaatcaa gccagtttca gttggtcagg cttcgcgcat atccggcgtc tcaccaggtg
     9001 atattgcagt tctcttggtc cacctgaagc gtcataaaga ggggaaagtt tcacgtgaaa
     9061 caaagacatt tttgatatag aaaatcgtag gcgacccata tgggtcgctt tctttggagt
     9121 gcatcctacg gatgccgcat cgtcacggac ggggcactcc atatataaaa aatggattcc
     9181 cgccttcgcc atgcacgaac ggtgcagggt tttcgctgga aggacataaa aatacgacat
     9241 aaaaattaag ccagcaattt aattgaccga taatttctat aacagggagg taattatgaa
     9301 tgagaaattc tattattcaa aaagattctt gcgggggatg ctagttcttg ggattggttc
     9361 gatgttcata tgtataacta cagtagtgct tgtgtccata aaggacctac ccttcatatc
     9421 aacggaaaat ctattatatt ccttgcaatt catatgcttt gccattgttg ctgctgggtc
     9481 ttttaggatc ctctttaagg tgaagagtaa gcctgcaatt actataacgg acaacaagat
     9541 agtctttggg ccatatattg ttaagagatt ttttggtgat ggtagaaagt tagaacttaa
     9601 cttcggacag attatttcag ctgttgtaat agacaattgg aatatttttg tccgccctct
     9661 ttttggtaaa aggaaagata ttgaggtgaa attcaaaata gacgacgata aagtgaggtc
     9721 gtttcatatc tatctcagtg tagtcgacaa cccagaaaga ctaattgaaa tcctcaagca
     9781 gcacataaaa tttgtgtgaa ttagcggtta cgtaggaaca gaacagtgtt ctgttcctac
     9841 aaaaagaaac catagcgatt ttttggagta tcccgccttt ggcgggacca agaaaaaata
     9901 aagctaaggc tttatagtcc aacttcttag gtagaacata aaagtcctac ggataaaagg
     9961 aaaaagtttc acgtgaaact tgaaacttta aaggttgtct cctcagagtg gggagcacct
    10021 ctttcttcag aacaaatagc taaactctct attttcgttg aacatctctt ggcttggagt
    10081 aggcggttta acctaatctc caaaaaagac tcgaacatag aggccatcac caaacatatc
    10141 ctcgactcat taaccctcct taggtttata tctatccctc ggaatgctaa aatcatagat
    10201 atcggctcag gggctggctt tccaggcatc cccctaaaaa ttgccagaga agatttagat
    10261 ttcagtttgg tggagtcaac tcacaagaaa ttcttattct tgaaggatat tgcccagaaa
    10321 cttgaattat ccaatttttg tatttttaac caaagagcag aggagttatc cggctgcgat
    10381 gaattcaaaa acaaatacga tttcgcaact gcgaaggcat tgaccgacct gactggaaca
    10441 gttaagattt gcttcccttt tcttaagaag ggaggagcct taatcgctta caaaggagag
    10501 aaattagaac aggagctaaa agagctaaaa caaagggtta gagaagaagc atttgaagtc
    10561 acaaaaagag agacgatttt catttccaag ataaatctga aaagaaacct ggtggctatt
    10621 gaaaaaagac ttgattttta aagggaagtt catttatctt gctagttatt ggataggagt
    10681 accgattggg gaaaataatt gcaatcacaa atcaaaaagg gggagtgggg aaaaccacca
    10741 ctgcggttaa tttagcttcc tgtttagcgg tggctgaaaa aagaacattg cttgtggata
    10801 tcgatcccca agccaacagc accagcggta ttggtttgga taagaacaag ttagataaga
    10861 ccatatacga agttttaatc aacgaaaccg cgattgaaga tgtaatccgt aaaaccgaaa
    10921 taccattttt ggaagttgcc ccctctcatt tgagactggt tggagctgaa attgaattgg
    10981 tgaatatgtt tgcacgggaa aaaaaacttg caagcgcact ggagccgata aagaataaat
    11041 acgacttcat tattgttgat tgtccccctt ctctgggcct tttgaccttg aacaccttga
    11101 ctgcggctga ttcagtttta atccccatcc agtgtgaata ttacgcctta gaaggattag
    11161 ggcaattgct taatactata aaacttgtcc aaagacatct caacccaagt ttgaaaatcg
    11221 agggagttct tttgactatg tatgacagtc ggcttaacct ttccaagcaa gtggcagaag
    11281 aggtaagaaa acatttttca gacaaagtgt acaagactgt aatcagtcgt aacgtcaggc
    11341 tttccgaagc tcccagcttt ggcaaaccga ttatccttta cgatatcctt tcaaccggag
    11401 cagaaaatta tataaactta accaaggaaa tattagatca tgagtaaaac agtattaggt
    11461 aaaggaattg aggctttgat tcctggtctt cccgaggata agtttgaggg aaaagggaat
    11521 ctgattcaac ttgaaatcaa gaaagtaaaa ccaaatccgt atcagccgcg aggcgccttc
    11581 gaccaaacaa agttggagga actggttgac tctatccggc agaggggttt ggttcagccc
    11641 ttagtggtca ggcgaaaaga ggacgggttt gaattggtag ttggagagag aagacttttg
    11701 gcagcccaga aggcaggatt gaatttcgtg ccggcgctcc tgttggataa actgaccaaa
    11761 caggaaatga tggagatggc tttggtggag aatatccaaa gagaggattt aaatccaatt
    11821 gaccaagcca aaggatacag ccgactcata accgaatgcg gggtttccca gaaagagctt
    11881 tccaaaagaa ttggcaagga ccgctcctct gtttctaaca tcttgcggct cttgaactta
    11941 cctgacaaaa tacagcgata tctgtcagaa ggcaagcttt ccgaggggca tgccagagcc
    12001 attttggcga ttccagataa gaagagccaa atatctttgg cagaaaggat cgtaagacag
    12061 ggtttatctg tgcgcagagc tgaagagctt gtttataaaa gaaaagaggc aagaaaggga
    12121 acaaaaaagt acaagacatc aaaatattct gatttggaag atagattaag ggaatacctt
    12181 aaaacagcgg ttaaaattag tcagaaacaa ggaaaaggta gaattgaaat agaattttac
    12241 tcagaagagg atttaggtcg aattcttgag ttattcaatt tgaagatttg atatgccaag
    12301 aaggtatgtt aattttatca tagttcctga agataaatca gaaggaataa agcttagatt
    12361 gtcaactctg gctgtgaagt tcctcttggg aattatcctg gtggtagtcg tggtaatggc
    12421 tattatgtcg atattttatg tcaaggcatt atctattgct atgttgaaca atgagttgaa
    12481 aaaagagaat caaaggctga gagaatataa ctccaaggtt gtgcaaatgg agaaggagct
    12541 cgaggactac aggtcatttt tctctagagt tgcgaattta gctggcgttg atcaagattt
    12601 gatagctaag aatggagaga gggtggctca agtgttggga aatacgttgg attccacagt
    12661 tgactactct atcggtccgc aggaggtgga cactcaagtt gcagatattc cgagcggctt
    12721 gcctttgggg ggatggatta gtagagattt tagcaccgag cataaggcta tagatattgc
    12781 agcaaaggag ggaaccgagg taaaagcagc tgcagatgga gtagtcaaat tcgttggctg
    12841 ggacgaaacc tttggcaacc tgattattct aaaacataaa ggcgatattg agacctatta
    12901 tggacataac cagaagattt tagtaaagcc caatcagaaa gtgaaaaagg gagagacgat
    12961 ctcgctatca ggaaacacag gcaaaagcac agccccgcac ttacattatg aaattaaaaa
    13021 ggatgataaa tcagttaatc cctggaacta tatggggaag aaacgctaga ggtcaaaatg
    13081 caaaaaagga aggaaaaaat ggagggaggg ttaaatacct ttgtgggcaa aggttcaact
    13141 atcaatggtg agttggaagt ggcgggtggt gtcaggatag acgggacagt taagggaaag
    13201 gtgacggcaa gcgagacagt tatagttgga gcagagggga ttattgaagc ggatattgat
    13261 actaagatta cggtggtggg tggaagggtg tccggagtaa ttactgctct ggataaagtg
    13321 gagctccagt ctaagtcaat gataatcggg gatatcacaa ctaagaatct ggtagttgag
    13381 cagggagcga tttttcacgg aaaatgcaat atgaaagaag ggatgacaga atagaggtat
    13441 aaaaggtagt gtttttgttg ataagtaacg agcctttgtg gaaatcagag taactatttg
    13501 atagatcttg tcttaaatga atgttactta gaagaaaaac aaagggttat ccacatagat
    13561 tctggtaagt caaggcagat ttgataatta ctcaatcttt gaaaatctta aaattaactg
    13621 aatacctcaa ctcgttacat attaaggact aagttgtggg aggggttgtc ttatcctccc
    13681 ttttgttttt caggtgaaat caaaacccag taataatagg tgaatcgtga gcctgttggg
    13741 tgcgcatatg tcaatctcag gtggggtttt caattcttta ttggaaggtg aacaagtagg
    13801 gtgcaatacc gttcagatat tcactaaaag tagcaatcag tggcaagcta agacattgag
    13861 cgcgaatgag atagatagat tttataaaga gcaagaaaga actaaaataa acccagttgt
    13921 cgcgcataat tcatatctta taaacttagg gtctccggac agaggtctat tagaaaaatc
    13981 aagagaatcg atgctaatcg aattgggacg ttgtgggaga ttgtccatcc cctatctggt
    14041 gatacatcct ggctcgcacc tgggagatgg tgagaaaaaa ggaataaaaa ggatcgctga
    14101 ttcaatcaat tggttgttgg gtaagactgc aaaagatgaa gtgaagatcg gattagagac
    14161 aactgccggc cagggctcta atataggtta tcgctttgag caattagctg agataatggg
    14221 cttaatcgaa gacaaaaaaa gagtgggaat ctgttatgat acttgccata cctttgcggc
    14281 tggatatgat ataaggaaca aggaaagtta tgaaaaaact tggaaagagt ttgatagaat
    14341 tctggggacg aaaaatctct tggctattca tatgaatgat tccaaaaaag ggttgggttc
    14401 aaaaatagat aggcatgaac atattggcaa aggggagatc ggcttggcag gttttaaact
    14461 tattatgaat gaccaaagat gggagaacgt ccccaaaatt ttggagacgc ccaaaaaaga
    14521 ggataaactc aaattagata agatgaatct ggcaactttg agaaggttga tagttaagaa
    14581 atagttttgg agtgcatccc gccgatggtg ggatgcatgc attgacacgg tcaatgcact
    14641 ccataaatat tagcgcgtag ggccgaagta aatctatcgc ccgaagagta ggagactatt
    14701 gaaaaagaat gttttgacgg ggctgaccca aaaggatctt ttgattcatt taaacgtctc
    14761 tcggctgtca ttgcgaggag tcccgatatc catcgtgacg acgtggcaat gctgaattaa
    14821 gttcaggatc gccacgctcc ccccgacaag tcggggtcgc tcgcgatgac aatggactta
    14881 tgggtcaacc ccactaccct ataaaaaatc taatacttaa gaacagaagg gataaaatat
    14941 gaagagaatt tggtttttgc ttatcttggt tttgtttttc tggtggagag gctcttattc
    15001 tatggttctt ttggatataa aggtggaaga gggtaagaag gggaaggtgg agagtttcag
    15061 cttgattgac cagggcacag tcgaactcta tgattcactt gaaacctcag cattcttggg
    15121 taacttcacc ctctcagccc aggccgcaac tttcgacagc gttaacttga cgttagatct
    15181 gtcccttttc actttgggtc cagatattca gagttacttc aaagaatatt tgctcaaaac
    15241 tggagaagaa actgtgctgc cagagcttag ggtaaaggat gatatcctct tcagggtaaa
    15301 gatcactcct catataaagg ctgaagagac aaatagatgc aatttgaata ccaaagatgc
    15361 ggagacctgg tattccgacc ccagcacgca tttctattat cactacattc tcgaaactct
    15421 gccggactac cactggaaca caaacaaagg ggtgttagag gatttttatc ataggatttg
    15481 ggataggttc ggtttttggg atcctgacaa gatcgattat tttttagtcc cctgcgagct
    15541 taaggagata tactgggata aaaggtatgg tcaggggctc gatcccacca aaaggaaaat
    15601 ctacgtgata tataatcaga gcacgcgcag cgtggatggg ccgggagtgg ggatgttctt
    15661 gttctataga tactgggggt atgcgccagc tatggtggtg gagggagcta cgggaaatat
    15721 cgggctggct cattatcaag cccaaaaact aaaaaaaaga ggtatgcttt ttagcttgaa
    15781 agaactggaa atcagtaaag attacaagtc caaacccaag gaaatcgctt tctatcaagc
    15841 cagctctttt ataaaatatt tagttgatac ttacggtttg gggaggttta agaaatttta
    15901 cgaagaggtt acggatttga cttttgaaca agccctattt aaggtttatg ggaaaaagtt
    15961 gcctcaactg gaaaaggaat ggttagcata ccttgatact ttcaaagagt atgaggcgaa
    16021 tttggtttac ttcgtcacgc ttaaaacgca ataccgacac tacgcagagg caattgacct
    16081 tttaaatgac ctcttggaga tgtcagaaaa caaggctggt tatttgagcg acctggctgg
    16141 catccattat accttaggag attacgagaa agcaaaagag tactacttga agttgttgga
    16201 tttgaagccg gaaaacccgc ttttcatctg tcttttgggg aacacttata atcttttggg
    16261 agattatggc caggcggaaa gcttgtatgt gaaagcaatc gggctggata ctagttacat
    16321 aaccccgctt ttgaagttag gcgaactata tctggcagaa ggggaatacc agaagggaaa
    16381 ggagcttttc gacaagggat tatccaaggg accgggcaca gaggaagcgg ctttgctttt
    16441 cttagggtca gcagagtgta agaagaattt gggccagccg gaagaggcac aggaagattt
    16501 ggaaaaaggg ctttaccagt gccaggttat gctgaatagt tttcctgaaa ggccagctcc
    16561 ttattattac agtggcaagg tttatctggc tatgggattt cctgataccg cagcatatta
    16621 cttggaaaca gcggccacac tggaggatat tccttataga cggggtaaaa ttcttttgga
    16681 cttggctggg gcttactctg cagttgggga tttggaatta gcacagaagg cttaccagga
    16741 gatattagac ataaactgtg gagcaaggga aaaagagcag gcggagaagc tcttggctca
    16801 acccaaagaa agaaaggagg gtagtaaatg agggtgttag caattagtgg tagtccggtg
    16861 aacggcggga acgtggaggt attgttggaa gagataagca tgggagcaga aagcagggga
    16921 gcgaaattca gcttaattca cttaaatgat ttagagattc taccctgtca ggcttgtggc
    16981 aaaagcctgg aaccaaactt ttgcttctat gcggatggga tgggattagt ttatgaaaag
    17041 ttattggctt gcgatatttt agtcttgggc tcgcccatct attttgattc tctttctgcc
    17101 cagactaagc ttttcatcga ccgctgcaac tgtataagac cgatagttga aacccctaaa
    17161 ggagatttca ttttcaaaaa tagattcaag aaacaaagaa agggagtagc agttctggtg
    17221 ggtggaaaaa ggggaaggtt cgaaggggct ttgactgtct tgagaggttt tttcaagtgg
    17281 gggaatttca agttggtgga tagtctgatt tataaacatg atgattggga ggtgggaggg
    17341 gtaaaaaaca ataaagaagt gatgaagagg gcttttgagt tgggggaggg gttggcacaa
    17401 ggcaaggcaa agtaaaagat tttaattttt ctatgtttaa ctgtcaggag tagctcctga
    17461 cagaacaata aaacctataa catttattga aggcgacatt cgcgaatgtc gccttcaaat
    17521 atgtaggggc gtattgcaat acgcccctat gcgctctaca tttcttgttc gcattttgtt
    17581 catgcgggaa ttggatttcc cgcatggctt caggaaataa tctcccctaa cccctctttt
    17641 tcaaagaggg gaacagaata atccctcccc ctttagcaaa gggggactaa tggttcgaca
    17701 tgctcaccat cctgagcgaa gtcgaaggag ggggatttga aaaagacaaa cacttgcgtg
    17761 cagtaaaaag atattaacct attggtctaa ttcccgtatg aatgataagt ctaagcgggc
    17821 ttcataaacc gctacatttt ttgttcgcac tttttcttta aattctttac caattccaaa
    17881 ttattttttt gcctttccct tagtttttca tccaagggat agcttaatat cttttcacac
    17941 tctttttggc attcctccat ttttcctaaa ttgtagaggg catttgccat ttttgtcctg
    18001 cactcgatta gattatagta attttttggc tggctttttt ctattttcac aagaagttgg
    18061 gagtaggagg cataagcccc ttcccaatca ccactttgat attgggcgga agccaaaggc
    18121 cataagaaaa gcttcccttg aggatacttc tcacacatat ccttggaatg cattacggct
    18181 aaaggatacc agccttcatt gatatagacc caaatcaaag cattagttgc cgcttctttg
    18241 gaaaagatgc tttttgtggc ggcaattttc aaaaactcta tcccctcttt tcttttatcc
    18301 cccaaaaatg gaagccactg aaaattcttg gtgacgatgc tactccaata atagtagcta
    18361 cccaagccca aataggcgtc agatagcgta gaatccaaat ctatcgcttt cttcaaatta
    18421 gatttagccc ttaagccatc tttcaaggca gaccacaact tgcccctttt cccctcataa
    18481 actgctaagt atccatacga gtttcctaag aaaagagggg cccaggcatc ttttgggttg
    18541 gctttgattt tctctttgct aaggtcaata ctcttttgga tattttgatg aaacctcttt
    18601 tcctcaaaat aatcttcttc atccatcatc ttaacctgca gaactgcagc taagaagaaa
    18661 tacccttccg gctcttgaga ataatctttt ataatttggc taaagcaact ttccgcttga
    18721 agaaaattgt caccaagcgt ggcatcaatt ccctgtaaaa tggattccct ggagacttgg
    18781 gcaaaagagt aattgatacc cagaaggaaa attaaacaaa aaagaaagaa ggtcttaagg
    18841 ttgattatgc tgctgaatgt ttttcttaat atcttgggct ttttgaatga actctttgat
    18901 atggtcgtcg gatttccctt gggtttgata tttcaaaatc tggtcaatct gctctaagca
    18961 gttagctttg tcccccatta cctgataaag ataggcaatc caatattttg tttccaccat
    19021 accacgagga gtggaatact tggaagcccc aaaggtctta agcaaagcct ggtaggtctc
    19081 ttgggcatct ttatacatcc cctttttgat ttgagtctga cccaagaacc acaaaaggaa
    19141 agggtcatcc ggctttatct tttttaagtc cgggccaagt gctaatgcag aatcatactg
    19201 ttgattctcc acatagattc tcaaaagccc atatttcgct tcatctcggg tgaacttgcc
    19261 cttttggata gccaatttgg tctgctcaat acccaacttt cgcttatccc caaaaaaggg
    19321 aagccaccac agcattttgg atttgatgct acgataaaaa ttgtaagctc ccaagccaaa
    19381 atagatatca taataggcgg aatcggtctc tacccccttt tccaaaatct ttttggcttt
    19441 gcccccatcc caaaaagctc cccaccaatc tccattaaaa gaccgataga tgccgcggta
    19501 tccgtaagag ccacccagat aaagcaggtc ctcggccgtg taattccccc tcttcatcct
    19561 ctcctcaccc ttttgtatag ctaaattaat atatttctca aactcccctc gataggattg
    19621 gtttcggtag tcatcaatga tagatacaaa agaaacggca acatacatat atccgccggc
    19681 atcatccgga ttaagttgta taagtttctt gaactctgct atcccctcat cgaatttgtc
    19741 ctgatgtata tagtctattc ccgcaaagat tatgctgtcc ctgtcctcgg catatagggt
    19801 aaaagggtag agaaatagaa ataaaagaat ggctaatatt tttgatttca tatctttaac
    19861 cagtaaattt tcgtctcaag ctttcccctg ttttctccaa aggatgagaa gagacctgtt
    19921 tttttatttt tacgtaattt ttcattccag ctttatattc cctaatccat tttctggcga
    19981 aagccccatt ttttatttcg gctaaaacct ttctcatatt ttgtttaact ttatcatcta
    20041 ttaccctttt ccctgacaaa taggagccgt attctgcggt cttgcttatg cgattataca
    20101 ttccactgat accataagat ttaatcaaat tcacaataaa atccaattga tgaacacatt
    20161 caagataggc atttccctcg ggtagtccag cctggaccaa gacctcaaat cctttcttca
    20221 aaagttcagt taatcctcca caaagaacgg cttgctcacc aaatatatcc ccaatggctt
    20281 catctctgaa agttgtccat aagacgcctg ctttggttaa gccaattcct ttggcataag
    20341 ccaaaacaag ctttttggct tgtccggtgt aatcctgata aaccccgaca aaacctgtca
    20401 cccctgtttt gtttttataa ttttccctta agatttttcc aggggaatgg ggggcgacca
    20461 atattacatc tacatttttg ggaggatgaa taagtttgaa gtgaatgcta aatccgtggg
    20521 caaataatag agttttgccg aggggcaggt atttttgtat tttcgaaaaa acttctggat
    20581 gagtatggtc gggaattaaa aaagagatga tgtcactttc ttgaaccgcc ttttcaatag
    20641 acaaaacttc aaaaccatct ttttgggcta atttgttgtt tttgcttttt gcccttaagc
    20701 cgataattac tttacatcca ctatccttca aattggctgc ctgtgccctt ccctgactcc
    20761 catagcccaa gatgccaatg gttttatctt tcagatattt taaatttgcg tttttgtctt
    20821 tgtagatttt caaatacttt ctccatccag ctttcttctt atcgctatca cttaatcctc
    20881 ttttaatctc acccgaagac cgtttgctgt cattgcgagt ccgtaggaca aagcaatctt
    20941 gatgttataa attgtcatat aagtcttccc attccggatt catcttggtt atcattgcga
    21001 ccttctttgt tctcgaacca cctttaatct gcttttctct ggtaatagca tacttgatat
    21061 cgttggaaac ctcataatat actaactttg taatattata cttctttgtg aaaccttcaa
    21121 tcaacttctt tttatgctca taaatccttt ttttgagatc gttagttacc cccgtataaa
    21181 ttactctatt gttctgattt gctattatgt aaacataata ttcttttgtc atattccata
    21241 gagattgctt cgttgttgaa gccgcctaaa aacgccctgc ttcatcatta tgtcattgcg
    21301 agcgaccgca gggagcgtgg tcccgccaac ggcgggactt cgtgatcggt atggactcct
    21361 cgcaatgaca gattgagagg tagtcgcctt tagcttcgta gatcaggtgc cctaaagtac
    21421 ctgacactct tccctacttt ttttaaatcc tctcttcact aaccatcatc ttccccctta
    21481 tctaatcctc ttctcttcct ttcccaccac tccatcctct ttttaatttc cttctcaaaa
    21541 ccaaactcag aaggggaata atattttctg ttcttaagat tctctggaag atagtcttga
    21601 tttacgaagt ggtcggggaa gctatgggga tatttgtaat cttttccata accgagcttt
    21661 ttcatcaagt cagttggcgc atttcttatg tgcaaaggaa ctggcagggc ttggtgtttt
    21721 tgaatgtcct cctgcacaag gccaaatgcc ctataaatgg cattgctttt gggtgcagtt
    21781 gctaaataca caactgcctg ggctaaagcc aattcccctt caggtgttcc caaaaaatgg
    21841 taagcctctt ttgccgcaat agttatctgt aatgcctgcg ggtcagcatt gccgatatcc
    21901 tctgaagcga aacggaccaa tctgcgagcg atatataaag ggtcctctcc gcctgctaac
    21961 atccttccca gccaatagag cgaagcatca acgtccgagc cccttaagct cttatgcaaa
    22021 gcggagatta tattgtaatg ctcctccccg gctttatcat aaagcaaaat ctttttctgc
    22081 atcacctctt gagcgatttc taaagttatt tttctggttt ggtctttttt atccggcttg
    22141 gtggtcagca ccgcaaattc caaagcagaa agtgccaccc gcgcatcacc atttgaggtt
    22201 tggacaatat attccaaagc atctgtggag agtttcacct tgaaattgcc caaccccctt
    22261 tggttatctt ctaatgccct cttcgaaatt agctcaacct gctctagtgt taaagaattc
    22321 agggtataaa ccttacagcg ggacaagagg ggagagatga cttcaaatga tggattttca
    22381 gttgtagcgc caatcaaggt gatatttccc ttttcaacat gcggcaaaaa cgcatcctgt
    22441 tgggctttat tgaacctgtg aatctcatcc acaaagagga tagttttttt accgtagaaa
    22501 ttcctttgtt ttgtggcttc attcatcacg tcttttattt cctttatgcc ggaagtcact
    22561 gcggagaagg gaatgaaatg agcatcggct gttttggcga taacatgggc taaggttgtt
    22621 tttccgcttc ccggtggtcc ccagaggata atggattgta tcttatcttc taagatcgcc
    22681 cttcttaagg gagagtcttt gcccaggata tgttcctgac caaccaactc atccaaattt
    22741 tgtggcctca tcctgtcagc caaaggcccc tttggtattt tggctttgag tttttcttct
    22801 tcaaaaaggt cagatttttc cttattcata ttgatcaaaa tactcacttt ggagagtatt
    22861 tacaagataa aaattcaacc tactaccata accaacaccc tagggtttct gtaggggttc
    22921 gcctgcccgg ccagggattt gcacagcatc ttgacgacat cgagtccacc ggtcgcacca
    22981 gagaacctag ccccctgttg tcccccttat cagggggatt gtgtcgcacc ctaaaggaca
    23041 tactcttgtc attctgactc cgccttggcg ggggaagaat ctaatggttt cgataagtta
    23101 gaaaatcgag attcttcgcc ccgataaatc gggactcaga atgacattat gacatggctt
    23161 caggggcaca ggaagcgttc ctcccctgat aaggggaggt taggaggggt ttatgcgacc
    23221 caaagagcct ttgtcattgc gagcgctagc gaagcaatct gaagtaatct cgggattgct
    23281 tcgtcgtcca tatggactcc tcgcaatgac agattgttaa gccagcaatt caattgccga
    23341 taatttttgt aaagggaggt aaatatggag gaaaaattct attatacaag acagtttgtt
    23401 aactcgcaat taattgccgc aattctattt ttagtaacgg caatcttgtg gaacgttgcg
    23461 tattctttgg aaggttggtc agtttggtgg agaatatttg gattgttata tactcttctc
    23521 tgtttacact atcttacgat atcaatcatt atcaaaaata agccctcggt tattataacc
    23581 gatgaaaaga tagttgccag atcgccattt tttctcaaac ataaaatcag ttttgagcag
    23641 attattccga ccgccattat tggtgaaaaa attaagctga aatacaagaa gccaaacaat
    23701 aaggaaggtt ctttttccat tgcccacctt cgtaagattg ataactgcga aagactggtt
    23761 gatatcctca agcagcgcaa aaatttgtat aggaaagtag ggacaggtcg cgacctgttc
    23821 ctacaactaa atcaacgaaa ctattaccga atctgccaca ggcagggctt ttttggtaag
    23881 acgcaaggag ctgccttgtt ttgataaaaa ccccagcccg gttaagtggt ttattttatc
    23941 ttttcttata tcttccaatt taactttgaa atctttctga aattgtctta ggtcgattcc
    24001 ttcagttttt ctcaatccca gaaaaatagc ttcagttatg agttgctctt tagatggctt
    24061 ttcttcaaac tgaattgctt tccccttctt tttcatcaac tctatatatt tcctgacccc
    24121 tcttaggttt ccccaacgtt tatctttgta aaatgaatga gctgacgccc caaaacccaa
    24181 atattcttta tgattccagt agttgagatt atgcttgctt tcaaagccgg gcaaagagaa
    24241 atttgatatt tcgtattgtt tatacccctt tgaagtcaaa taatcaattg cccaaagata
    24301 catctggcga actttctcgt ctgttagtag tttaattttc ccctcttttt tcatctggta
    24361 gaatttagtt tctttttcaa tggttaaact gtatgtggag atatgctgag gggaaagtcc
    24421 aactgctttt tttaaagaac ttttccagga cctaagagtc tgctcgggta tgccaaatat
    24481 caaatccaag ccgatattct caaatccgcc tgctttaagc aagttaaagc tatgaatggc
    24541 ttgatctgca ctatgaattc tgcttaagtt tttgagctcc ttatcattaa aactctgaac
    24601 tcctaagctt accctgttaa aacccatatt tctcaatccc cttatcctgt tttcatccaa
    24661 cgtattaggg ttggcttcaa tagttatctc gcaatttggg gttaaggtac agtccctctt
    24721 aataacttgc ataattttat ccaattgctc taatttcaga acagtagggg tgccgccacc
    24781 taaatagatg gtgtcaattt taagacttga aaaactctta atctcataca aaagcgctga
    24841 aacgtacttc tccactaaat ttcggtcaag ggggatggag gcaaaatcac aataagggca
    24901 ttttgaaaga cagaaaggta tgtgcagata tattgaaaga gacatcagaa atgcaaacta
    24961 tagcaaacgg attaaatgtt gttacatatt agagcaatat gcaatctatc ttaaaatccc
    25021 cctcccttcg actttgctca ggccttcggc tctgaggctc aggctcgaag agatggtgag
    25081 gacattcggc tgagcggttc gaccgtcctg agcggctcac cgtcgaagga ctcagtcgaa
    25141 gcccatatcg aaccatcaag tccccctttt tcaaagggga agtcctcttt ttcccctctt
    25201 tggaaaagag gcccgcttcg cccgtctcgc cgatggctcg ccgaggcggg ccgaatcgag
    25261 gcgagggcga ggggagattc attgaacgta aattaatgcg tttgtattat ctaacccggc
    25321 ttccaattct actccaacga accctgtcag ggatatgata cagattatag aagaagattt
    25381 caattaaggg gaaaatgtat gggcttctta actttggcgc attgggatcc ggagcccagg
    25441 agaatattac caaatctacc tttcctttga gatttccttt ttccacaaat ccccaggtcc
    25501 ttgagtcttc actcttatcg cggttatctc ccaggacaaa gagctcatcg attggcacct
    25561 gtattggtcc aaaattgtcg cgggtggagt aaagagcagg tattatgtat ttgtccgtga
    25621 acttaacctt ttcagagtcg cggaacatcc ttccatccac agatacaact ttgcctacaa
    25681 tctccacagt ctgtccctcg gttgccgcgc atcgttttat gagagtcttg gtgagattta
    25741 atggatatct aaagacgaca atatcttctg gttgcggagc tctgaatctg taagctaatt
    25801 tattgaccac cacccagtct ccgaccagaa gcgtatcctc cattgagccc gtaggaacgc
    25861 gatatatctg caccacaaag ttacccaaga ggaatatgac tattgcgatc aagagcaata
    25921 tgacaaaaag ccgccttctg ggagaggcga tgttcctttt tttgctggct aattgaacca
    25981 tttcccctct tttcccattc gttagtttta tcggaagtta tgttcaaaaa attaacaact
    26041 ggagatttat ctttctatct taagaagggc taagaaagct tcctgtggta tctccactgt
    26101 gccgatctgc ctcatcctct ttttcccctc tttttgcttt tccagaagtt ttcttttgcg
    26161 ggtgatatct ccaccatagc atttggcagt cacatttttc cttatggggc taatcctctc
    26221 tcgggagatt atgcgcgagc cgatagcacc ctggattatc acctcgaaaa gttgtcgtgg
    26281 aatgagctgt cttagtttgg tgcataattg tttgcccaga tagtaagcct tttcctgatg
    26341 gacaatgatt gagagagcat ccactggtgt gccatttatc aaaatatcca ttttaaccaa
    26401 cttagagggt ctaaattcca ataacttgta atccatagaa gcatatccac gtgaaatggt
    26461 tttcaatctg tcatagaaat cgaaaattat ctcggacagg ggtatgtgat aatgcaggcg
    26521 gacacggttt ttgtccgcat attcggtggt gatgtagatc cccctgcgct ccgtggccaa
    26581 tttcattatg ttaccgatgt attccgaagg agagattatc accaattcca catacggttc
    26641 ctctattttc tccatttggt tggagggagg aagagcggcg gggttgtcca ccaaaatgac
    26701 ttttccatcg gtgcgggtta ccttgtattc aacattggga gtggtgttta tgatggatag
    26761 accatattcc ctctgcagcc tttcctgaat gatctccata tgtagcattc ccagaaagcc
    26821 acagcggaat ccaaaaccta aagcgttcga tttctccggc tcaaaactca aggaggcatc
    26881 attcaatttc aatttggcga tggaatcttt cagctcctca aagttttccg ccaggctggg
    26941 atagattcca gaaaagacca taggctttat atccctgaag ccgggcaggg gctctatggc
    27001 tggattggcc gcatcagtta tggtgtcgcc gaccttagta tcagccacgt tttttagatt
    27061 tgccataata taacccacgt caccagctga gagtttctgt tgaggcacga acttcatttt
    27121 caaatatcca atctcctcta cctcatactc tccgggatga gagaaaaatt ttatcttggt
    27181 ccctttggag actgtgccct ctttgactcg aatatagggt attgctcctc tataggaatc
    27241 gaaaatggag tcaaatatca tacctcttaa aggagatgag ctttctccct cgggcggagg
    27301 gaccctcctc actatttcct ccaaaacccg gtctatccct ttgcccaatt tggcgctggt
    27361 gaggatgata tcctccacat taactcccaa aagctctatt aagtttttct ttacagtttc
    27421 tatttgggca ctgggaagat cgattttatt aataaccggt atgatcgtca ggttattttc
    27481 caaagctaaa tagaggttgg agatggtctg agcttccact ccctgagagg catccactaa
    27541 aagcaatgcc ccctcacagc aggctaggct gcgcgaaacc tcataagtga agtcaacatg
    27601 gcccggagtg tcaatcaggt tcaagatgta aatttcccca tcttttgctt tatattccat
    27661 tcttatagcg tgcgacttta tggtaatccc cttttccctt tccaggtcca tatcatccag
    27721 gacctgctca cgcatctgat gaggggagac agtcccggtt ttctccaaaa gcctatctgc
    27781 caaggttgat ttgccatggt caatatgggc gataatggag aagtttctta cgttggagct
    27841 tttcacgatt tttccttaag tgaaagatta taataaactt ttggccagaa gtcaactttt
    27901 aaaggtaaga gcttagatgt cttatagtat gggtgagctt ccggaatgga aaaaatgtca
    27961 ttgcgaggag tcccgagagc tttcgggacg acgaagtccc gcctttcgcg ggacttcgct
    28021 ccacacgaca gaatcaggac tcgcaatgac agaaaacgat ttgcagatta ttttatagaa
    28081 ggcgacccat atgggtcgtc ttctacttct aatacgatgc attcgtatcc ctgccttgcc
    28141 ctcaaggcag gagataggcc cacgttgagg gcaacgtggg tttacggttt tagagatgag
    28201 ggatcgcctc tgggaaataa cctcttcgta gtgcggggaa tttatccccg cacgggctgg
    28261 gacgaacctg tcccgtgccg gatggtgcgg gattcccagt tctacgagag aaattaaaaa
    28321 tgtaggggcg aggtgacctc gcccctacat taaaaatcgt cctgacttat cgggattact
    28381 acattaagat aggagtaggg acaggtcgcg acctgtccct acatataaat atcccccctc
    28441 tttttgcctt gacaaaggtt cagatttggt tatttttatg aatcaagccc agacttgggc
    28501 ttgattatta gtttattctt tttggcggct tatgattaag cttttattgg ggttgggaaa
    28561 tccaggaaga aaatatagtt caactcgaca caacttaggt tatcgtttgg ttgagctttt
    28621 agccaaaaga agcaagagaa agtttaagta cggtaaggga agttatattt tcactgaagt
    28681 caaaataaaa agaaaaaaag taattctggt caagccgcag acttttatga accagagcgg
    28741 catagcggct aaagattgct tggatgattt cagtttaagc ccggaagagc ttctggtgtt
    28801 atgcgatgac atgaatcttc ctttgggtaa attgagaatc cgaccaaaag gagcagatgg
    28861 cgggcataat ggcctgaaat cgataatttc tcttctgaat acacgggatt ttgcccgttt
    28921 gagaatgggg atagggcttc cctctcctga aattagagcg gaagattacg ttttgcagga
    28981 tttcaggtca attgaaaaag atgaagtagc aaagatacta aaggatgcca gctcggcagt
    29041 ggaaacagta atattccaag gtataaccga aagcatgaat cgatttaact gaaagaataa
    29101 tggaagttcc ctgctctgga ttttttccgg ggccaaaagt ttttggtccg aagggaggtg
    29161 aaacttgaga aagtacgaga cggttttcgt tttagattcc tctttagaca aagccaccac
    29221 agaccaggag gtgaaaagag ttgaggattt aattgccagc cgggagggga aggtcataaa
    29281 ggtggacaga tggggaatca gaaggctggc atatcccata gccaagaagc aacagggtta
    29341 ctacactttg atcatcttcg agggagggtc agatttactc ggtgaattgg aaagaagcta
    29401 taaactgaac gaatcctgtt tacgctggat aaccgtggta tgtgaagcgg aagaggagcc
    29461 agcggaaaag agcccatcca aagaagaaaa agaccaagtg tagtattttt gttttggggc
    29521 taacttataa ggaggaaaat aaatggaaga attaattcgt aaaaagggag tttgccgttt
    29581 ttgcgaagga agagcaccga gaatagacta caaggatgag aaattgctta gaagattcat
    29641 caccgaaaga ggaaggatac ttcctcgtcg cataaccggc aattgcgccc tgcatcagcg
    29701 ccggttatcc attgctatca agcgcagtcg acacttagcc ctgcttccct tcacttcgga
    29761 agtctatcgc taaacagtgg aaagagatac gaatagctct ttgaaaaata aagtgtggat
    29821 ctgggcatct ttgtgtttgc ttctgactct gggatatgcc ttctcatccg ggatatgggt
    29881 agtcagttat tttttgggta ttgcacttgc cctatgcctg actatctttt tttggagaag
    29941 caaaaggtac ggcctaattg gtgcagtctt tttggcagcc ctgttttatg ctctaagggg
    30001 aggaaattcc gttgtttttc tttctaaatg tttacttccc gcactgatat taagtttagg
    30061 cctaaagcat aagagaaacc cgagtttaat catctttctg gcaattatcc cccatttagt
    30121 gattttagga ctaacagttg cccactacgg ggatatagtt ttcattttca gggaccagtt
    30181 aggggagtta gcccaaagga tagccggagt cttcgagatt tttggtataa atgcgattgt
    30241 attacagaca aagatatttt ctatctttga tactctagct caacttattt ttggtttcga
    30301 gcttctttcg agctgggttg aggtattctt gatttatctt ttaatccagc ttattggaga
    30361 caagctggga tgggatacga ttaagctcac ccgctttggt ctttggcata gccaagagag
    30421 atttggttgg gtggctcttt tgagtttggt tctttttttg gtaggaggga agctgtttga
    30481 gatagtatcc aaaaatgccg ccttggtttt tggattttgg tatacggtcg taggcttttc
    30541 aataagcgag tacttctttg gaaaatcgaa agtgaatatg tggattaaga ttttgttcta
    30601 tgtttttgtt ttggtaactc aaattctttc ttttgtgctt ttgtccttgc tgggattttt
    30661 tgacagctgg ttagatttca gaaaattagg gaagaagtca ttatctaata aaggagtaaa
    30721 tatatgaagg ttatcttaag ggaagatata ccaagcctgg ggaaatgtgg agaggtgata
    30781 gaggttaaac ccggctatgc caggaattac ctcattccta agaatttagt cattatggct
    30841 acgaagggaa gcctaaaagc tataaatgag gtaagcaagc aaaaggagct gagggagaat
    30901 aagaagaaaa aggagagcct taagctcaag ggtgccattg aaaagatatc ctgcactgct
    30961 gaggtgaatg tgggcgagga agataaagtg tttggttcgg tcaccagcca ggatatagtt
    31021 tcactcttaa aggagaaggg ttttgacata gacaagaaaa aactagagtt ggaaaacccg
    31081 attaaagtcc tgggagttta tacacttcca attaaacttc atccagaagt agttgccaat
    31141 ttgaaacttt gggtagtcaa aaaagaaggg agataaaatg gccatggttg aggtcaaagt
    31201 gaacggatta gctctggata tagtaaccaa ctccccagtg gtgatactct ccgatattga
    31261 gtcagacagg cttctgccaa tctggatagg gcattatgaa gcctgggcaa ttgctatgga
    31321 gctttccggt cttgcttcca agcggcccct cactcatgat ttgattaaat ctgcaatcga
    31381 gctgatggga gggaaggtgg agaaagtgga agtgactcaa ttagttaatc aaaccttcta
    31441 tgctaaggtt catatccaga agaacggctc acttatggag ttggatgcca ggccttctga
    31501 ttctatagct ttagcactta agacccaggc accggttttt gtggacgaga agctgttcga
    31561 ggcaaaaagg gaagagatgg aaggtaaaga gatgggcgat atggaatctt taaaggaacg
    31621 gctcaaaagg attaatccgg aagatttcgg taagttctct ctttaaaccc aaattatgca
    31681 ttagaagcga agcgaactaa tgcataattt gggataaatc tcccctcgcc ctcgcctcga
    31741 ttcggcccgc ctcggcgagc catcggcgag acgggcgaag cgggcctctt ttccaaagag
    31801 gggaaaaaag aggtctcccc ctttgaaaaa gggggattaa gggggatttt agaatagatt
    31861 gcatattgct ttaatatgta acctttatct tctgtcaagg gcaaaaaacg ttatatgcta
    31921 aagaaaccat tcatattcct cttaattctt gcgattttga ttacggctta ttctattcac
    31981 gctttagctc aggcgccgga aaatgagttt aagcaagccc tgtcgcttta ttccgatggc
    32041 aattacaaag aagccagggt gaggttcgag aagttgattt ccacctatcc ggcagacgaa
    32101 catttatcga ttttcaagtt tatgctctcc aagtgcgatt actatctggg agattacatc
    32161 ctggctcaga gcggattcag aagttttgta accgagtttc cgcaaagcct atatttggga
    32221 gatgcccatt tcatgctggg gaatattgat tatctagagg ggaacaaatt tgaggcggca
    32281 gtggaatacc tcaaagcata cgattatgcg gagaatgaag agctcaaagg tttagcctat
    32341 aactcactta ttcctcttct gaaagtgggt ttgagcttaa gtgaacacga taggttggtt
    32401 cggcaaagca ggggaagaaa attggcggat gaggtattgt ttcaattggc tcaaagggag
    32461 cttgagcgta aaaactttgc tgaagccaag gactttttgg agcagtatat tgaaaattat
    32521 cctgaaggca gtcatcttaa agaagcagaa gagcttctga aacccgtggc ttcgccccga
    32581 ggcgagtatg tatctaagcc cactcaagat atatatccga gacttccctc ggcttcgccg
    32641 agaggcgagc gtcccgagac gagcaagata ggcgttttgg taccgctatc cggagtttat
    32701 cagacttacg gtgagaatct tctgaaaggg ataaagctgg ctgtggcgaa atactatccc
    32761 caaagaattg agctaacagt caaggacacc caggagaacc caattgtaac cgcccataag
    32821 accaaggagt taattgagga gaacggcgtc atagccataa ttggtccatt gaaaagtgat
    32881 tgtgccgcct ctgctgcggc tgtggctaat tgcctttctg ttccgctggt ctgtccagcc
    32941 gtatcagagg aggggataac cactttgggc aaatatattt ttcaattaag caccagcact
    33001 gagaaaatgg ggagagcaat ggcacactat gccacaacta gtatgggact gacggaattt
    33061 gtcattttgg cgccggatga ttcctatggg agaaacacag tgagtggttt tgaggaggag
    33121 gtgaaacact taggagggaa aattgccgct tgccagttct attctcgaga aagcacagat
    33181 tttggaaatg aattgagagc catccgggat attcttttca aggaaaagga aaaaagggag
    33241 ggcggaatag ataccaccaa atacgttgac atccaaggca agcctttacc tcttgatgcc
    33301 attccagttc aggtagacgg atttttcctg ccgggttaca gcgaggacgt tatcctcatc
    33361 gctccgcaaa tagccttcac caagatagaa acgcatcttt tggggagtga ggggtggaaa
    33421 aataaaagga tcatcgagct ttccggagat tatgtgcaga aggcagtttt cgtctcagat
    33481 tcctatgagg atgagaatga ctcttctgac tttgtttctt cctatgaaaa aacctatgct
    33541 gaaaaaccgg ataaggtagc tgttttaggt tatgacagta tcaagttctt gggcttggcc
    33601 ttggagggaa agagttggac ctctcaagaa ataggagact gtttaagcaa agtgaaggat
    33661 tttgacggag catcgggcaa gtttttactt aatcctcaac atgagaacga gagcttttcc
    33721 atatttacca tagagggaaa gaaattcaaa aaactagaat aggagagaaa ttggaccaac
    33781 aggaaagaaa agtggctttg aaggtcttca tagacggcaa ggaaagggat gttacctttg
    33841 atgaactgac tctgtccaat aatctggctt tggagtcttt gcttagggtt ttagcccgaa
    33901 agaaagtcgt tgattcgaag gagcttatgg aagagatgga aagggtgaga aaagaaagaa
    33961 tgccagatct ccctttggat aagaaataga tatgtctgaa caagttatgc ttaactggaa
    34021 agtccatcca tttaaaagaa acaaaaaagt ttcaatcgga gtgattttgc ttttattttt
    34081 ggtctggggc atagtgtatc ttgccacagg cagtttactt tttttggcaa ttgcagtcgt
    34141 tatgttgttg ggttcccttt cagggttctt cctgccgacc gaatatgagt tttatacgaa
    34201 taagataaaa gtgaaatatt ttttagccag cttggagaaa gagtggaaga attatcgcag
    34261 tttctatata gataagaatg gagtgctttt gagtcctttt aaagaaccat ccagattgga
    34321 gaacttcaga ggggtatatc tcagactgga cagaaactca gagataaaag ataaggttat
    34381 agattttatc aaatctaaga tggataattc tgttgacggt tcacggtaga tggttcatag
    34441 ttaaaccatt aattacggac tgtgaaccat gaaccatagg tgaattaaat atgatttttc
    34501 gtcagaaatt ctcgcttccc gactttaagg aaacagattc aaatctcaag acccaaaatt
    34561 cggggaatct gacaccagag caggaagagc ttttacgtaa ggtggcacag aaggttgtgc
    34621 gctggaagat gtcggtccca gccattcttt tcctggagtc agtcaagccc ctaaattacc
    34681 ttggaagtca gatgatggtc ttctttgaac cgtttgttca gaccgttttc acctggaaag
    34741 attatgacca atacagaatt attatggagg aaagggaaaa tgtcgaaaaa ttgttacaga
    34801 agatagagga gttggatgcc atagaaaaag aaaaagaaaa agaaggaaaa aagaaaataa
    34861 aggaaaaaag aaagaattta ccctggggca aaagaatttg gagttatata agcggaaggt
    34921 gatagattga aatataataa gttgtcattg cgaggagccc cgatggcaat cgggacgacg
    34981 aagcaatctt gaaattaaaa ccgagattgc ttcgcttccc ctacagggtc gctcgcaatg
    35041 acagaatata tgtgcagggg tgaagtttat ctattgcctg agctggtaga gacgcaaaat
    35101 tttgcgtctc tacaaaccac agggaattca tccctgtgcg gataatcatt tcgaaaatgg
    35161 aggtaaatat gaaaaggatt ttaattgctt tgttagtgtt agttttgtgc tttagtttga
    35221 ctttggccca agaagaaaaa ccactctccg gcaagaaggt tgtgatgata attgccccta
    35281 aagatttcag ggacgaggag ttattggagc caatgactat tctcttaggt aaaggagcag
    35341 aggttgctgt tgccagcagt tcccttgata cgagcgttgg gatgttgggt gcgaaaatga
    35401 agccggagat tttggtttca aatatcaaag ccaaagactg ggatgcaatt gttctggttg
    35461 gtggacaggg agccagagag tactggaatg ataaagtttt acataccatc ctgaaagatg
    35521 cagtaaaaga agataagata attggagcga tttgcatcgc tccggtgacc ttagccaaca
    35581 ctggcatatt gaaaggcaag aaagcaacgg tgtatgaagg tgtggcagac aagctaaaat
    35641 caaaaggggc aagttatacc ggctcggatg tacaagagga cggcaaacta ataactgcca
    35701 atggtcctaa ggcggctggg agatttgcgg aggtgttagt agaagcttta ggaaagggac
    35761 aaaaagtgtg tccaaaaaca ggtttaaaga cctgcccttt gcattaagac agtttaccct
    35821 gcaccattac ggtgcagggt ttagaagttt aaaaagtttg tagttgctcg tctgcccttg
    35881 gcagggcagg gatttatcga acaaattctt gtcggataaa tccgaccgct acaataaagg
    35941 agattgcttc gtcgtccttc gacaaaagct tcgggactcc tcgcaatgac agtatatggt
    36001 attgtcattg cgagcgtagc gaagcaatct atgcagttgc cgcgatattc atcgggacgg
    36061 ttttagataa aatcaaattc gtcccataaa tgtggcaact acattaagac attgaaaaca
    36121 aatataataa ctcgaatgga caaacaacaa ggagcaatat gaaggattta aaacctgaaa
    36181 atgtcaatgt aattttagcc acggattgtg gaagcaccac caccaaagcg atcttaatcg
    36241 aaaaaaaggg aagcggatat cgcctcatcg ttcgcggcga agctcccacc acggtggaag
    36301 caccttttga ggatgttact atgggggtct taaatgcagt gggagaggtg gaggaactga
    36361 ccgggagaaa gcttttggat gagaacggta gaatcatctc gcctgccaag ggaaatattg
    36421 gctgtgatat ttatatctcc acttccagcg cgggtggtgg tctgcagatg atggtggctg
    36481 gagttgttcg cactatgact gcagaatcag cggagagggc ggctctgggt gcaggtgcga
    36541 ttgtgatgga cgttatctcc tcaaatgata aaagacttcc tcatgagcag attcaaagaa
    36601 tcaggcactt aaggccagat atgattttgc tttccggtgg aatcgatggt ggaaccacca
    36661 cacacgtggt tgagctagca gagttgattt ctgcggctga ccccaaaccg agattgggaa
    36721 gcggatataa gcttccggta atctatgccg gaaatgttaa tgcgcgcgaa gcggttaaag
    36781 agactttgtc cggaaaaaca gatttggata ttgtggacaa tattcgtccg gttctggaaa
    36841 gagaaaatct tttgcctgcc agagagaaga ttcacgacct tttcatggag cacgttatgg
    36901 ctcaggcgcc ggggtacaaa aagcttatga cctggaccga tgcaccgata atgcccactc
    36961 ccggagcagt tggtttaatc atcaagatca ttgcggactt ggagaagata gaggctgtgg
    37021 gtgtggatat aggaggagcg acaactgaca tcttttcagt ttttcagggg gttttcaatc
    37081 ggaccgtcag cgccaatctg ggtatgagtt attcggtctc aaatgtattt gccgaaaccg
    37141 gacttcccaa tgttatgcgc tgggttcctt tccatatgga tgagcgggat ttaagaaaca
    37201 gggtaaaaaa taaaatgatt cgtccaacca ccattcccca gatgatggag gagttgattt
    37261 tcgagcaagc cattgccaaa gaagcgttaa gattggcttt cattcaacac aaatcgtttg
    37321 ccacggtctt gaaaggtata cagcaacaga ggaccattgc ggatgcattt gaccagactg
    37381 ccagtggtca aaccctggtg aatatgatga gtctggatat cttagttggc tcaggcggag
    37441 tgctctcgca cgcacctcgc agacaacaag ccgccttaat gctgattgat agtttcctgc
    37501 cagaggggat aacccgctta gcagtggata gcatatttat gatgccgcaa ttaggagtgc
    37561 tttccaccat tcatcccaaa gcagccaagg acgtgtttga taaagattgc ttgattcatt
    37621 taggaacttg catcgctcca gtgggcggag gaaaagaggg gaaggagatt ttaaaatata
    37681 agtttaagct acctagtgga aaagaggagg aaggggtttt gaaatacggg gaaatgaaaa
    37741 ggattctttt ggatgtagga gaaaaagcag aagcgagcct ggagccctat aggggttttg
    37801 acatcggcaa aggaaaggga gcaaaagtcg agaccaaaat aagtggagga gttgccggga
    37861 taattttgga tggcagagga agaccgttta gtttgcccac agatgacaaa atcagagtga
    37921 ataagctcaa agagtggatg ctggctctgg atatttatcc caaagaagcg ctggagagct
    37981 tgtaaattta catttaaagc cagcgggaac tccccacctg ttaaggtggg aataagagca
    38041 gtttgtgagt ttttgtgtca ttcctgtccc gcatttctgc gggataaact ccagtctcaa
    38101 gacgcagaat tcgcgcagag cccgtcccgt actggatacg ggaaatccag aaaaggaaag
    38161 caagtatgga tccccgatta aaccctcggg gatgacaaga cgtagtaagg gcaattcatg
    38221 aattgccctt acaaacagca agaaccacac ccgtccttcg acatgctcag gatggtgagc
    38281 aaggtcgaac cataaaggcg cgggagttga tatattgaaa ttgagattaa cgaattactc
    38341 atataatcat aataaataaa caaaagaggt ttttatgggt catgcatata cccccggttt
    38401 aaaagtaaca gaaagagtcc ttttaataaa aaacagaatc ttgcctttaa aaggggaagt
    38461 attagtgaag gttggggata aagttcatcc cgatacggtt gtagccagaa ctcttctgcc
    38521 gggaaacgtt gagccggtga acgtggctaa taaattgggc attccagcag aggatgtgga
    38581 gtttcatatg ctcaaaaagg agggagacaa ggtagaagaa aaagagacca ttgcagaaag
    38641 cagaagtttt tttggtcttt ttaggtctga tgtccattcc ccaattgtgg gaaaaatcga
    38701 gagcgtctcg catattaccg ggcaggtatt gattcgggga aatccaattc cggttgagat
    38761 aaaggcatat ctggaagggg aggtggtgga ggtctttgag aaagaagggg tggcggtcag
    38821 cacctggggc tctttcattc aggggatttt tggaataggc ggggaaacgc atggacagat
    38881 aaaagtgtta accaaggata acaaggaagt gctgactgac aagctgattg ataaaagctg
    38941 taaaggaaaa gtgattatag gcggaagtat agtgaccagc aatgccattc atcaggcgat
    39001 ttcagttggg gcttgtggaa ttgtggtagg tggattggac gacaaagact taagggagtt
    39061 tttggggtat gacttaggtg tagccataac cggctcagag gatttaggga taactctggt
    39121 cataaccgaa ggtttcggtc agataaatat ggcggagaag acctttgaac tctttaaatc
    39181 caaagaggga aaggaagctt gcattaatgg ggctacgcag attcgggccg gggttattcg
    39241 tccggaggta gttatcccct atgaaggaga tgtgcataag aaggaagaga aagagaattt
    39301 gggaaatgtc ggcttaacca cgggttatcc agtgagggtg attcgtcagc cctattttgg
    39361 ggaattagga aaggtggtag ctttgcctcc gcaactgcag aaattggagt cggaatcaaa
    39421 agctcgagtg ttggaagtcc agttcaagga tggcagaaag gtggtagtcc cacgggcgaa
    39481 cgtggagatg ctggaggagt agtaaagcag ttttgagttt tgagaaaatt ctttaggtgt
    39541 agaggcaagg aatcctcaac ccctcttgat ctccccttat caggggagca accagcagag
    39601 tccccctgac aagggggaca acagggggtt agtcaggagc tttggggttg atgttctgtc
    39661 aggagctact cctgacggat ataaaacaga aaagagaaat atcaggacta tggttcgaca
    39721 taacccaaaa gagagtcttg agtttcaaga gacgggggct aataaagtat tgtgcaggct
    39781 aaatccttta actaaatggg ggttgataag atatattgat ccctaggcca cggcataagg
    39841 atttttttgt tgaggaggta ataaatgaaa ttagatgata tcaagatctc tcgagccata
    39901 atcgaatcct ataaggataa actcgtccga gctttagatg tagatgttgc tatagttggc
    39961 gctggccctg cggggatggt atgcgggtac tatctagctt gtgccggtaa agatgttgtc
    40021 atattcgaaa ggaagctatc agtgggtggt ggtatgtggg gcggtggagc gatgtttaat
    40081 caaatagtga ttcaaggaaa agctaaaaag atcatggatg agtttgatat ccataccgaa
    40141 ctctatgaga agggttacta cattgcggac tcgattgaag cagtttcaac tatctgctcg
    40201 aagaccgcga aggcaaaagt taagatcttc aaccttctaa gcgcagagga tgtgttggta
    40261 aaggataagc gggtatgcgg tttggtcctt aactggaccg cggttgagat ggccggtttg
    40321 catgtagacc caatcaccat gcgtgcgcgt ttcgtagtgg atgctacggg ccatgcggct
    40381 gagatagtgc atatcataga aaggaagtcc ggtatgaaac tgaagacggc aacaggtaag
    40441 atccaagggg aacagtcgat gtgggcagag gcaggtgaag atgcgatcct tggaaattct
    40501 aaagaggtcg cctcaggttt atatgtctgc gggatgtgtg ctaacgcggt gtttggtgca
    40561 ccacgcatgg gtcccgtatt cggaggcatg ctcttatccg gagagaaggt tgcccgtgag
    40621 ctaattggga agttatgcca atgatggcgt aagaatagct cgaggatgcc tgcaaattga
    40681 ttcaggggaa ggaaaagtat tttgagattt ggtttgataa cacaaccatc tttagtaaag
    40741 caatttaaga gtttagaggt ttataggttt agagaattat gtggcgattc gatttgcaca
    40801 gtatcttgac gacatcaaat ccaaaaacct tgttcttgtc attgcgagga tcccgccaca
    40861 agcgggagac gaagcaatcc ctttttcgaa atcgagactg cttcgttccc tacgaggact
    40921 cgcaatgaca tataattact tggcaggcga cattcgcgaa tgtcgccttc attgggtcgc
    40981 attgaccatg tcaatattct atgaacaaga aatggacccc aaagctggac aggataagcc
    41041 tctgtctatt atctgacaaa aaggagggca cttgaccgat tcaaatcatt tcaatctcaa
    41101 aaattgtctt attgggcttg ccgccttaac agtggcaatt cttattatcc tatatgcggt
    41161 caaagtctgg ctggtggata aagccccatc cagccattct tcagatagcg gttcaataac
    41221 actaggaagc agctcttttg aaaaacgatc ctccaaagcg attacttggc aggaagctga
    41281 caagcattat ggggaaaatg taacaattga aggcaggatc gcaggaacat acaattcagg
    41341 aaaggtttgc tttcttaatt ttgacacaaa ttacaagaac tcatttaccg cagttatctt
    41401 cgcatcggat tttcaccttt ttcccgcaaa tcctgaaagt tactacgatg gtaaaaaagt
    41461 ccgcataaaa ggtcgcatca aagaatatag tggcaggccg gagattatcc taaacaacct
    41521 tactcagata gaggtgataa aataaaccca gatttcgcaa tagcaaaatc tgcccagagg
    41581 gccgctacat cccgcttcag cgggattagt cccgccttag gcgggaccag agggggtccc
    41641 tagacaaaat ttgcgttgca cctttttggt ataattggtt attatgtaag caccgactcc
    41701 tgaacaactt gaaaacattg ctcattttct aaggcaaatt gtgggataaa aagggggact
    41761 tttgggacga aaactctgcg cgactcttct tgcactaacc ttatccctaa tcccagccgc
    41821 aggccgctca agcgattttg tcctaagtag ttttgttgaa gccggaagct ggtccacaac
    41881 acaggactgc gaagaggatc tggatgaaac tttctcctat cagtattatc atctaagatt
    41941 tgaagagaaa ctatccacca agtcaaaata cagcctcagc tcctttttat accataaaaa
    42001 ttacacttcc ctcgattccc tggacaattt gtcccgcacg ctaaactttc tttggtccta
    42061 tgatgcggta aaatcaggcg ccaagacctt cagtttggat ctggcgctgg gatatcgggg
    42121 aaagcgctac gaggacaaca aagcctttga ctatgacaag gtgtacatcc ttccttcagc
    42181 cgtctttgtg ctaaaggaca atttaaaact agatttctcc tttggagtgg aaaacttcaa
    42241 ttacctgtca ggcaaaaaca aagatgagtt caagcttttg ggcgcaatcg ggtgtgagag
    42301 gtttttcgaa aagaaacagt tgaagttggg cctgggttac aaaatagaaa attcaagtaa
    42361 aagccggcaa ggccgcaaga aattaaaaca ggatgtctca ataggggctg tgctcaagcc
    42421 caaagcctca cttttgtctg aacttgcctt aaaaatgacc tttggccaaa gggacaccaa
    42481 agacgatgaa gaccaagacc aagattacga ttattgctac agatccttcc tcatccgaac
    42541 caaacacaag ctcaaaaaga aagtaacctt tggctttgag tgtggatact ttaagaaaac
    42601 ttatgtcagc gcccttttgg ggtaccattc ttaccacatt gccaacaatt gggactacca
    42661 aattctggat aacgaaaagc acaaagtaac cttggggcta tggcttgaac acaaaagcgt
    42721 ggattatatt ggcggccaaa gaagcgacta ccgaaaggag actatcaaaa ccaaagtcaa
    42781 cttccagacc aaaaaggaat ggaagcttgg agctggacta gaagagaatt tttacatata
    42841 ccaaacacca gccaaagaca aaaacaggac ttattttgta ttttcgctgg agaaagcatt
    42901 tttctcaagg cgttttcttt tgcaggctga ttttaaaaca agatttactg actacaaata
    42961 ccaaaacgac caggaacaga aggcagttag attgggaatt gaatataggt tttaaaggta
    43021 cggaacaggc atggagcgtt ccgtacaaaa acggcgacat tagaaattgt gcccccgtag
    43081 ggacaggtcg ggtggaacat cccgctagaa ggtatgatag ggagcagaca tgccgccgtg
    43141 gtaatgaacg ggcatggcgc cgtagggaca ggtcgagtgg aacatcccgc tagaagcgtg
    43201 gaacctgtcc ttacagagac gtcaatatca aacaatcccg gttattaccg gttcgtatga
    43261 atccgtgggt acaaaatccc cagagagaat atttattgac atacgaagct ggcaagagta
    43321 tattattttc agtttatcaa aaagcaccca acggggttaa atgtgaaaga aaaagtaata
    43381 gcaaaaaaga cgctttctat taaggaaata gacctcataa cgcgtttgga attctatggg
    43441 aaagaaatct ataccaaagg ggagattgtc tctttctgtg gtggtagcaa acccgcggca
    43501 tacttgataa agaagttgtt ggaaaaaaac agattgagga agattatcaa aaacgtctat
    43561 ctttttgttc caatgaaggc tccaggtggc ctctgggctg ggaatgagta tttaattgcc
    43621 aaagctttgg caagaggagc tcgctattac ataggatatt ccgcagtatt taattcctat
    43681 ggctttacag atcaagttgc gcaaactatg catgttatca atgatagata ttcaatgcgt
    43741 aaaacagttt ttggggtcag gtataagttg ataaaagttt tgcccaaccg gctttacggg
    43801 ttagagactc gcaaaataga caacgaggat gttgttttcc cgaaaaagga acgggcaatg
    43861 atagatgtgt ttgagtttta taatgtaagg aaagcataca gcatattaac cgaccaaata
    43921 tctgaactta atacgcccct tcttatcaac tatctgacgc aatatcctgt tctgggagtg
    43981 cgaagacgcg tgggctattt tctcgaaaaa ctgaggatca atagagacct tttgagtaaa
    44041 ataaaagttg gcgaaaaagg atactccccc ttatacgaca taggctcaaa tcaaggaaag
    44101 atcaataatc gatggagaat tgttatcaat ggatagggac aagctggcga gaataatacc
    44161 tgtcattgcg caagaatata acttcagatt ggaaatcatt gagaaagact tttatttgac
    44221 cctcattttg aatagtgttg agtcgcattt aagcgacaaa atcgtcttca agggcggaac
    44281 gctcttaaat aaaattcatc ttaattatca cagactgagc gaggatatcg attttacata
    44341 ccttagtccg gatggcttag acacccgctc gaaaagatca catgctttgg ccccgataag
    44401 agagaagatg cccgaactgt tgaagctttt ggacctgaaa agcgaaaaac ccgaagggca
    44461 aggttttaat aactccactc aatatgtatt caacatttcc tacccttctt tgatttccgg
    44521 aaaggacgaa aacatacagt tagaaatttc attaagacaa acgcctatag ataagcccgt
    44581 gcataatgtc atcgcacatt tttataagga tccttttacg ggtgaggatt taattcccaa
    44641 gaataagata ctctcgcttt ctttaaatga agcggttgcg gagaaactca aagcttcaat
    44701 aacaaggaaa gatgcagcta tccgcgatta ctatgactta tggtatatat cggagaccaa
    44761 attcgacttt gaagataaaa agttccttta catattcaag aagaagcttg aatccgagaa
    44821 atacagagcc aattattcat ataatttcgg cttggatcaa aaaaaggttg aattgcttca
    44881 taagcaaata gagacggaat taatacctgt tattcatgta ggtgaaagtt ttgatcttga
    44941 taaagttttc aggcggttta ataggatctt gcagaaaata tggcgtgcgt gatatccgta
    45001 aaaagatagt agggaacggt cgcgtggaac atcccgctaa aagcgtggaa cctgtcccta
    45061 caaaaacggt gacattagaa attgtgcccc cgtagggaca ggtcgcgacc tgtccttaac
    45121 agagacacca atatcacaag ttgtttggcc tcgagttggg agaattgagt tgacagagca
    45181 atgaatttgg agcatataaa tagggtaggt aagattggta agacagctta tccgcgagca
    45241 aagcaaattc gcgcaacgaa gataggacga tggtgggtta agcctgagga tttagaaagt
    45301 tttattaaaa gcaggagcaa cattcaatga tgaaaatgac aaaaattaga gacaaattag
    45361 atttaattag aggggaaatt atttcatcta cagcaagagt tgaatttatt ctgggctata
    45421 agttaagaaa atattttttc cccaaggcca ataataaagc aacgattctt ttctggaacg
    45481 ttattaatac tcctttcctt aattttgata ataaaattag tctttatgaa tcaatcccgt
    45541 attttaaaaa attaaaaggt cattcagaga ttaagaaatc tttaaggttt atccaaaaat
    45601 tgagaaataa aatggctcat tgggaactgg atgaaaagga aagcgacttg aagaatattg
    45661 ttatgtttac attggtcggg aaatatagaa aaataacaat taatgatagt tcaattgaag
    45721 attataggaa gcatgttaat tttcttctaa acaaatttgg ttatagtaga tgaatttatc
    45781 aggcttcggt acttggatgc attcattaag gcaaggacaa ataagtgaga aagatttatt
    45841 aattctgttg aatcacatgc attgggagag aatatagatg ctgacagatg gaacgttgta
    45901 tggataaagg tgcgcatttt tacaggtgtg attttcaagt tcacactccg cgtgatctaa
    45961 attgggctgg tcccgaggcc gttacacccg aagaaagaaa gacatatgcg gaagaactga
    46021 ttttggtttg tcgatccaaa ggtctcgatg caatcgcgat cacggatcat catgacttcg
    46081 ccttcttccc atacatcaaa caagcggcta aagatgagct tgacgacaac ggtcaacggg
    46141 tttctgcaca caaaaggatc gttgtctttc ctggcattga agttacttta actgctccga
    46201 attgccaagc gttacttatc ctcgacgcag acttcccgga gaatctcctt ccaagcgttt
    46261 taaccgcact ggccatcaca cctgcgccgg cagaacattc aaagcacgcg gatgtacagc
    46321 gaattcccca gaatgttgtt agcgatctga cggatttata tcaaaaactg aatagtcacc
    46381 agcaactgaa gaatcggttt atcgttttac ctaatgtgag cgaaaccgga tgcggcacaa
    46441 tgctacgatc cggatttggt aatttctata aatcaatgcc atgcgttggt ggctacacgg
    46501 acggcccagt gtcgcaattt ggtaccggga acttatcgat tgttcgagga gaaaatcgag
    46561 agtacggttt taaggcgatc ggaatctttc aaacatctga taatagaaaa cgcaatcacg
    46621 aagacctcgg caaatacacc acatgggtta agtggtctga gccaacagcg gaggccttaa
    46681 gacaggcgtg tttagcaaaa gaatcaagga tatcgcaaga agttcctttg ttaccaccgg
    46741 tattcgtgac gtcaattgat gtttctaaca gtaaattcat gggacaaatt tatctggaat
    46801 ttagccagca atacaacgcc ataatcggtg gtcgtggaac tggaaaatca acaatcttag
    46861 aatatcttag atggggtttg tgcgatcagg tttcagaaat tgtacaagat gagggagaaa
    46921 ttcctgggta tcaggaaaaa agaaggaagc ttatagaaaa gaccctgctt ccgtttgatt
    46981 ctacggttca gatttcattt gtcaaaaaca acgttcccca catccttaga agaaaatccg
    47041 gaacgaacga gatgtctcta aaaattggat cgggggaatt tcaaccttgt ggtgaggagg
    47101 atgttagaaa cttgctcgca atccaagcct acagccaaaa gcaattaagc agtgtcggtg
    47161 tcagcattga ggaattgaaa aggctgattt atagccccat caggcaaacg cttaacgagt
    47221 ttgatttaaa atttgagaaa ttgcgctccg atatcagaag ttgctatgaa ctatggcagc
    47281 aaaaaaaatt gatcgcatct gaaattgaga agggcgagct tgagcttaag tcattgactg
    47341 agcaagtgga aggtctccga caagggctaa aaggaatctc tgatcaagat aaggctacaa
    47401 ttgcctccca taagcaatat gaaattgttg agcaacatgt tgacggttgg cacggagaac
    47461 tggcgacggc aagagaggca ttagaaacca tcaaaagaga actcgccgag tatcccacga
    47521 ccgtacctga tgatgtcaaa ttgcctgaca ctgagctcat agttgttacg gatattcaga
    47581 agacactcaa agacattttt gatgagatta acgcgacagt tgattctttg ggtgacagat
    47641 taaatcctaa gggagaggtt ctgaccaagt taaacgattt gatagatagt tggaaaggtg
    47701 ctctcaatca acataagcga caatatgaat ccgcaaagca aaaatcaact tcacaggaat
    47761 cgaccttaaa gcaaatccag ataatcgaag atagggttaa agaaataaga agatctttaa
    47821 cggaaagaaa gcaagccatg ctaaaaacag gagatcccga cacaaaattt aacgtcctta
    47881 aagaagagtg gtttaacgcc cataaggaaa aggctgattt gtttcaagcc caaagtgaga
    47941 agttgaatga attatctgat gataatttaa aagcaactct aggcagagga aagggcacgg
    48001 taaacctaga aaacgtgcta aaggagataa tctcaggttc aaacgtgagg aaggagaaaa
    48061 tagaatcttt gtgccagaga attaaagagg cgacggatcc tattcaacaa tggaatcaaa
    48121 tcttggccga gtttgaagca attgctcact tcgatccaac caaaaccccc gccgcgactt
    48181 taccggcaac accgatttta agctctatcg gttttagtgc aaacgatcta cgcaaaatag
    48241 cagagaaaat aaatagcgaa aactggattg atctatttct tgttcagttg gacgatctgc
    48301 ctttgtttga gtatcgtact cgtgaaggtg agtatataga attcacagat gcttctgccg
    48361 gtcaacaggc aacgtctcta atgagggttt tattaaatca agaagggcct cctcttataa
    48421 ttgatcagcc ggaggatgac ttggataatc ggatggtttc ggatattgcg actttaattt
    48481 ggaaatccaa gaaaacgagg cagttaatat tcacaagcca caatgcaaac attgtggtta
    48541 atggcgatgc agagttggtt gtctgttgcg gatacaaggt tacgggtgat caatcaaagg
    48601 gtgaaattaa gaatcaggga gcaattgata ttgaggagat acgagaggaa attatagagg
    48661 ttatggaggg cggcgaggca gcatttaagc ttcgcaaaga aaagtttgga ttttgatagg
    48721 tgggaaaaat tattaaggct ttattcaatt aaacagtagg taaaaaatgg cgatgaagaa
    48781 atctgaactt tacagctctc tttggaaaag ctgcgatgaa ttaagaggcg ggatggacgc
    48841 ctcacagtat aaagactatg tgttggtttt gttgtttgtc aaatacatct ctgataaata
    48901 tgctggcgtg acttacgccc cgatcactat tcctcaaggt tcaggcttca aggatatggt
    48961 ggcactcaag gataaggcga ctattggcga cgacatcaac aaaagaatta tccaaaaaat
    49021 tgaagaggca aatccatcgc ttggaaatat tgccgttgat tttaacgatc cagcaaaatt
    49081 agacaggtgg cccacagctc tgctgtggga caattgcgga acggtctcgt ggaacatccc
    49141 gctagtggcg tggaacctgt ccttacagag atgttgacag agcaagcggg attgttctat
    49201 aatatagaac agttgtatga tatgagcgaa cttgatctat tataatttca agaattttgg
    49261 gtagggtcaa tgattattgc agaacgacaa gttaaacctt ttctcaaatg ggccggcggt
    49321 aaaggtcagc ttatcgaaca agttgtttca tacttaccct cttctctaaa ggaaggaaga
    49381 ataaagaaat actttgaacc ttttttgggc ggtggggctc ttttcttctg gttggccaaa
    49441 caatacgagt tcaggtcgtc ttacttgtac gaaattaatc ccgcagtatg tctttgttat
    49501 caagtcatca aaaagaatgt gagaaagctg gttaaggaat tacagcagtt ggaattagaa
    49561 tacttttcgt ctactgaaaa tagaaaagaa caaatttact acgatagacg agaagaattt
    49621 aatgcgtttt tacacgggaa ggcatccaat ggcgttgtcc atcgaactgc cctgttaata
    49681 tttcttaata aaacgtgtta taatgggctc tatcgcgtga attcacaagg cgaatttaat
    49741 gttcccttcg gacgatataa gaaacctacg atttgcgacg aagacaatct tcatgctgtt
    49801 agtgaattac tgcaaaaggc agagatcgtt tgtggggatt ttgcgctatg tttggactat
    49861 gcggacgaca aatcttttgt ttactttgat ccgccatatc gacccatatc ttcgacgtca
    49921 agctttacga gttattcaaa gggtgttttt gatgatgccg agcagaaacg cttaagaaag
    49981 gtttatggtt cgctcgataa gaaaggtgct tgcataatgc taagcaattc tgacccaaag
    50041 aacatgaacc ccgaagataa tttctttgat gaattgtata acagatatcg tattgtaagg
    50101 ttgaatgcaa caagacttat caattgcaac gccaaccaaa ggggcgttat caccgagata
    50161 ttagtgatga actattaaaa aggatgtatg atggcgaata gcaaatcttg gaagaaaatt
    50221 gttgacgact atgaaatctt agggcacgac tttgacaaat cgccgtttgt gctttctgca
    50281 aatcaaataa agagagcatg tcaggaattt aaagaaaccg gcgagaaaga ggttcgtatt
    50341 ttgtgcaaac aggataatcg tgaagaccga ccagacgtct tcattgaaaa tggtcttttc
    50401 ctgctacctg tcagaaatgg cctctacaat atcattaaag gagagggata tgttgatatt
    50461 ccggaaataa agaaagagac tgctatttat tcttcaatgc ttgattttaa attagagaca
    50521 acagaagttg ggaactctga aatgcaacat cttgattatg cttatgctag cagtctaata
    50581 cgcacattca tggacgattc gtcgcttgta ttgacaataa ggggaagaaa gtacacgccg
    50641 gaattcgaat tttctattgg aaagcatcag attaaagtag caagcgtaca aactgaagtt
    50701 gacgctggtt atgaggggaa aaatcaggtt gttttgattg aggcaaaaaa tttcagtgca
    50761 acaaacatta ttattcggca actttactat ccctttagac aatggcaggc gcgaacgaag
    50821 aaaaaagtag tcactttatt ttttgataaa aaccctggcg ataattctta ttcaatttgg
    50881 cattttgaat ttacagattc ccagaactat aacagcattc ggttagtaaa atctggtaaa
    50941 tatagaatca tagaaaaata aatgagtcat taagcgcaat ggataagaaa aatactgatg
    51001 aaagattgga acctcaaaca actaccgtct ggtcatttcc aaatcgtggt aagtggctgt
    51061 cgcataacgc caagtatcgc ggcaactggg caccacagat tcccaacaat ctaattcaac
    51121 tttattctaa ggaaggcgat atcgttttag atcctatggt gggaagtggg acgacaatga
    51181 ttgaggccaa gtctcttggt cgcaaaggtc ttgcttttga cattcacccc gaagttgtca
    51241 agttggcgca ggatgcttgc atgttttcgt cggaacagaa gcatttcgag ccagctattg
    51301 aacaagggga cgcgcggaat ttgaaatcac tgaaagatca tagcgtcgat ttcatcgcaa
    51361 cgcacccgcc ctatctgaat atcatcaaat acggaagaaa ggacgttgac ggtgacctct
    51421 ctgctataag cagcctcaag aaattctgcg atgaaattga aaaggttgct ggagaatgtt
    51481 atcgtattct aaaacccgga aaattctgtg ctattctgat tggtgatacg cgtcgaaagc
    51541 ggcattatgt tcctcttgcc ttttcggtca tgcaacgctt tctgaagcca ggatttgtat
    51601 tgaaagaaga cgttattaag attcaacaca actgtgcgac gaccagatac tggaatgcac
    51661 aaataaagga cttcttgctc attatgcacg aacatttatt tgtttttcgc aagccagacg
    51721 ttgatgagga caaatttctt tataaggata gcatctataa tgtttaggta aaggcaggtg
    51781 gcccacagcc ctgctgtgga agaatttcaa taagacccaa gaattgtttg tagttcacac
    51841 atagaattgg tataaatata gatagctcca agttccctct aagacaagga ggcttttatg
    51901 aaagaagtaa cctcagaaat cgacggattg gtggttcgtt ctagcgggat atggattaaa
    51961 caaaaatatt actacttgaa aagatacctg gatatttttt caaaaggaat gaagaataga
    52021 tggaaaggaa atttaactta tattgatcta tttgctggac ctgggcgttg tttaatagaa
    52081 tctattaagg aagaagaaga cggttctcct ttaatagcat tgcagtacga ttttaagaaa
    52141 tacgtttttg ttgaagaatc aaaagaacta attgaggctt tgaaaacaag atgtaaaaat
    52201 tcccccaagt ttagcaggat tgaattttta gaaggagatt gcaataatgt tgttgatgaa
    52261 attattcctc aggttgaccc agccggttta aaccttattt ttattgatcc taccggaatc
    52321 gatatccatt acaagaccat tgaaagaatt gttaaaaaca accgatctga tttgttaatt
    52381 aatgttcagc tcgggatgta tatcactaga aattttcttg cctataagaa aaagggtgat
    52441 aattcaaaac tgggattatt cttagggggt aacgttgatt gggataaatt aaagaatccc
    52501 caggatgcag ttaagttgta taaagagcgt atcaaggggc tgggttatca gacagtagaa
    52561 ttcaaagaca ttactattaa aaatacccta aatgccccga tgtacttttt attatttgct
    52621 tcaaagaatc caaaaggcct gcatttttgg aaagagataa ctaaaaaaga tcattctggg
    52681 cagttcgagt tgttttagca agaaaattat ctttcagtat aattcgtgga agatggtata
    52741 atataaacat ggaaaaaatt taccgtaagt ctcttttgta caaaagtgaa gtcgaatacg
    52801 cagatttttg tttgaatcat gtagaaggat gctcccatgg ttgcaaatat ccgtgctatg
    52861 cttatatgat gaaaaagagg tgcgggattg ttaaaagcta cgatgactgg tgcaaaccaa
    52921 aaatagtagg taatgcgtta gacctcttag acaaagaaat tcctaaatat aaaagcaaga
    52981 tcaaatatgt gcacctctgt ttttctactg atccatttat gtataatcaa gaagaagtga
    53041 gcgatttaag cctgaaaata atcgataagc ttaatttaaa cggtatccgt tgcactgttc
    53101 tgacaaaagg cgtttttcct gaagaagttg gtcataaaac tgtttttagc aaaaacaacg
    53161 aatatggcat tacacttgtt tcattggatg aaaatttcag aaagaaattt gagccaaatt
    53221 cagccccttt tcgcaataga attcgttcgc ttgaggatat acacaaacaa ggttttaaga
    53281 cctgggtgag catggaacca taccctactc ctaatttgat taagcaagat ttgcttgaaa
    53341 tattggaagc ggtgtccttt gtggaccaga ttatctttgg caggttgaat tacagtgtga
    53401 aatcctcgga gtttggacat gcaaaggaat tctataacag tatggcctcg tcagtgatta
    53461 agttttgcag gaagcacaag atcaaatacc atattaaaga tggaacccag aagctaagtg
    53521 gtggacaata gtctcttttt aaggcactac tcggcagaca aaacagggat tgcttcgcta
    53581 aagctcacaa tgacagctga atactgcaac ttagtacgcc accaaagttt tagcgagtga
    53641 agggaaaagg gaccttaccc gatgggtggg gtacacttgt tatcatggga gaaaaacatg
    53701 actagggcag ataaattttt gtctcttttt aatcttattg aaaaaaccct tcacaaaata
    53761 agtcctcgcg agcctggtga gggttttaag gaaatgatta atcgtctcag caagtctaac
    53821 aagattattt gccaatataa atatgattta agggactacg ccgaccttag aaatgcaata
    53881 gttcatcagt ttacagggga agccatcgcc gaaccgcacg aagatgttat tgccaatcta
    53941 caaacaatcc atgatatttt ggttagacca ccgacagcca ttgaaatttc ctccaaacct
    54001 gtttattgtt gcgaagcaaa ggataaaatt gttgatgtag taaaaacaat gacagccaaa
    54061 gtttacaccc atgtccccgt ttacgaagat gatagattcg ttggtgtttt ctccgaatcc
    54121 agtattgcaa ggtggttagg cgaaaatgcg gagaaagacg gatttctttt gaatgaaacg
    54181 agagttgggg aactaaaaca attctttgag aaagataatg accccttcaa ttcttacaaa
    54241 tttgtttcaa aggatattga cgtatttact attatggact atttcttatc ttttgttaat
    54301 gaaaataaac gtttaggagc tatttttgta acgaatagcg gcaggagcag tgaaggaata
    54361 ttaggaatta ttacatcatg ggatttacca agaattgata aatagaagag aaaaggtact
    54421 atattcatcg gggctcagta atacttacca ccccaccggc aggtggccca cagccctgct
    54481 gtgggagagt tgcggaacgg tctcgtggaa catcccgctg aaagcgtgga gccgttccct
    54541 acagagaagg ttgcgtgggt gattttaggg tagggaacag gcatgcctgt tccgtacaaa
    54601 acggccgcgt ggatgattgt gatgtaggga caggtcacgt ggatgatttt agcgtaggga
    54661 acagtcgcga ctgttcccta caaaaagatg aaggcgatgt ttgtgtatga aagtcaatac
    54721 aaaaccggat aaactatggg atgtggtggt gattggtggt gggccggcgg ggatgatggc
    54781 agcggggagg gcggctgagt tgggtcgctc ggttttgctc gtcgagaaaa acgcaattct
    54841 tggcaaaaag cttctcctta ccggcggcgg acggtgtaat ctttccaata ataaaccaga
    54901 acttcgcacg atgctggcaa aatacaaagg cgttgaccaa tttcttttct ccgctttttc
    54961 acagttcggc gtagaagata cgcttgagtt ttttaagcgg caagggatgg cgaccaagga
    55021 ggaagcggag gggaggattt tccctgtttc aaataaagcg caaacagtgt tggacgtact
    55081 ggtgagctat atgaaagagg gcggtgttga attacgaacc aactctgtag ttacaaggct
    55141 ttgggttgat gcttccaata ccgccacaca gcacatccgt atccgactta aggacaaaac
    55201 agagttgttg gcgcaatcct gcgttgttgc cagcggaggc acggcacgtc cggcaaccgg
    55261 ttcaactggg gatggatttg cgtggcttaa aaaaatcggc cacaccatca tccaggacga
    55321 cgttgcgctt gtgcccgtcg cgcttaaaga tgcgtgggca aagaaactga gcggagtaac
    55381 gcttatagat attaagctca ccacttttca aaacaaagag aaacaggaag ttgggaaagg
    55441 tgaactgctc tttacgcact ttggcatcag cgggccaaca gttttagata tgagtaaaga
    55501 aataggagaa cttttaaaat acggcgaagt taccatcatg ctagatatat ttccgaaact
    55561 tgatcacggc gcgctcaggg ataaactgca gacgcttttg aatagccaga gtaacaagaa
    55621 gttaaaaaat acgatgaata agctcgtccc tttagcgctt gtgccggcga tactccgact
    55681 gactggtgta gatggccagt tacccagtca tagcctgagg cgcgagcata ggataaaatt
    55741 ggttagtgtg atgaaggcca tcccgcttaa tgtcaagggt ttgcttggtg cggataaagc
    55801 gattgtctgt tctggtggcg tagcgctcga agaagtagat ttcagaacca tgcagtcgcg
    55861 cctggtacca aatttatata ttgtgggcga tttactgaac atcgatcgcc cctcaggagg
    55921 ctatagcctc cagctctgtt ggacaaccgg ctttgtggcg gggagcaata cggaacggtc
    55981 gcgtggatga ttgtgatgta cggacaggtc gcgacctgtc cctacaaaaa cggccgcgtg
    56041 gttgattgtg acgttaggaa cggtcgcgtg gatgattttg gcgtagggaa caggcatgcc
    56101 tgttccctac ttgagagcgt ggaaccgttc cctacagaaa caccaatatc aaaaaatgcc
    56161 aatcaacaat gcgaaataga aaaccaaacc gtttgaaggg gtatgattat tcgcaaagtg
    56221 gtgtgtattt tgtaaccgtg tgtgcacaaa accgcattga atggtttggt gaaatcgaaa
    56281 acggggaaat ggccttatgc ctggaaaaag aagaatctgg atgtatataa tcggttaggg
    56341 aaagaaaaat gataatccgt tgcacggcaa gatttttaaa agaaatggga ttaaccaagg
    56401 ccgatctcac cagcaacact tcagaagaat cagagttggg cgagtggtat actaatctgt
    56461 tttttgtcga acgtaagaaa tgtgtgattt ttactaatgc ccgaacttta tttacgttca
    56521 tatggtttga tgttgataga tcagagatca ggaatcttgg taagcttttc cgcattggtt
    56581 tcggcaaggc cctattggat gaagatttcg atggtgcgtt gatacagcat ctcgttaacg
    56641 agtgccgaga tatacgattt gccaaaacac aagacagaag cgtactcggc gttatggtgg
    56701 atcacgtcaa gaacacaaaa tggatggtca tacgcgatgg cggattagaa aaatccgatt
    56761 tctctgaaat aataaaacaa cttaatcgca caccgctttt aacgaaagac tttacttatt
    56821 ctattgagga gcttgggaag gttttgggag ttgctgtaga tacgaaactt aattttaatc
    56881 cgtctaatac gtttttgtca tcaagcgcgc ccgaaataag ggacaataag gaacaggctt
    56941 aagagacaat atggttgatt gtgacgtacg gaacggtctc gtggaacatc ccgctgaaag
    57001 cgtggaaccg ttccctacaa aaacggccgc gtggatgatt gtgacgtacg gaacggccgc
    57061 gtggatgatt gtgacatgtg gaacggtcgc gtggaacatc ccgctaacag cgtggaaccg
    57121 ttccctacga aaaccgaata tacctcgagg aggtttgagt gccatttaac ccgaaattca
    57181 ctattaccgc gaaaatcaac aaggcgctgg tagaaattga acgggtgcgg gggttcttag
    57241 atgcgttgaa gctcaaggat gactggttta ttgatatgca acaaaaagca ttaatcttag
    57301 agtcgcacca ttccacgcat attgaaggca cagccttaag cttgcagcaa gccaagtcca
    57361 tcctggaagg caaaaaggcc aatggtgtca gggctgacga taaaagggaa cttacaaatt
    57421 acaagaaggc catggatttg gtttcaagat atcttggcaa ggatgatcca atcaccgaaa
    57481 gcgctatctc tgggcttcac aaagttactg tgaaaagcgt ccgtggcgga caggcagacc
    57541 cgggcaatta ccggaaaatc cagaattatg tggtcaattc ccgcacccgg aaagtaattt
    57601 acaccccgcc gggtccgctt acggtcccgc ttctcatgcg cgagtttgta gattggctca
    57661 acaaaaccga ggaggtgtcg ccgattcttg ttgccggcat agcgcagttt cagtttgtgc
    57721 atattcatcc ctttgttgac ggcaatggcc ggatggcccg gcttctctca actttaatcc
    57781 tttacaaaac cggctatgac ttcaagcggt tatttaccat ctcggagtat tacgacaaag
    57841 accgtccaag ctactataag gccatccagt cagtgcgtga gggcaatatg gacatgaccg
    57901 gctggctgga gtacttcgtt tgtggcctgc gttcgcagat ggaagaaatc caagctaaag
    57961 gccagcagtt aattaaacaa gatgtcaaac ttcagaagat taaaaaactt gggattggcc
    58021 tgcgtcagga aaaggcggtc aagcatctaa ttattaaagg tacgattacg gttaatgaat
    58081 atcaggagat tgcctcatgt gtccgacgga cagcccagag agatttggaa gatttagttg
    58141 acaaaggcat catccaagcc gtggccaaga gcgctaccga tcccacaaag tattacgttt
    58201 tactgtgaca cgttactgtg acatactgtg acatgctact gtgacatacg gaacggtctc
    58261 gtggttgatt ttagcgtagg gaacaggcat gcctgttccc tacaaaaacg gacgcgggga
    58321 tgattgtgac atacggaatg gtcgcgtgga tgattgtgat gtagggacag gtcgcgtgga
    58381 tgattgtcgc gtagggaatg gtcgcgacca ttccctacaa aagataaaat cattatcgga
    58441 attggtcggt gcattcaaaa caacgtcatc aaagcatatt cgtcaattgt gtttgcctga
    58501 gtttggatag caacggtcat tctacgacca tgtaatacgt gatgagaaat cattatgtga
    58561 tgtctagatt tacatacaaa ataacccacc aggctgggat ttagatagaa ataatacgga
    58621 gaatttgttt atgtgattca ttttgtgctg tcactaaata taggatcata gtataatcta
    58681 taggagggcg aagatggagc tagcacttaa gattataggg atgtttttgg gggttttggc
    58741 ccggacttat gtcccttact tacgaaaact aaaagagggg aaggaaacca agttcaaaaa
    58801 atgttatact ctctcaacag tggcgtcgct tgttttgggt ttgatcataa ctttacttgt
    58861 ctttccgcaa tttgatattg ccaaagcggg cgttggcctg gaagcttcca tcaagttatt
    58921 ctgtcttgcc tttggtttcg gttttggatg gaatagcata gtttcggaga cgatcaaatg
    58981 gacaacagga ggcaaaaatt agatcgccac gaggaggttg tgctctcaaa tcctcgaaaa
    59041 ccgattggtg atagatttct cttgacttaa cttattgttt ctgtaaattg gagtaaataa
    59101 ttttctaaac cctaggatga tgaatgggag atagtcctgt ttcgattgta gtccttttgg
    59161 gaaggggcaa gaaataataa tcaaatcacc ttgagaatta aaggtgattt tttcttttta
    59221 aaatatggtg ctttcaaaga aaatgaactt tctgtttatc ctcataatat tcgcatcttc
    59281 ttgttcactt gtgtgggcag accagcctcc aatcccacca actgaagtta agggctctga
    59341 tacaaaaaac gatgccggtg gaagcataac tctcagctgg aagaggtcgc cagatgatgg
    59401 ggcggggaaa aacaatattg tctcttataa ggtattaagg tcaagccaag aagaaggatt
    59461 ctacatcttg ataagtgatg tgaccgctgg cgcagaacag tttctggata acaatgccaa
    59521 agatgggata aaatattttt acaagataaa agctataagc aatgaaggtt tttctttgtc
    59581 cttgcccagc ccagccatcg tgtccaaggc acagtggttc gatacaacaa agatctatat
    59641 actggcgatg tcggtaattc tcttttcggc tattctttat ttcatatcta aagctaaatc
    59701 gggtaaggag cttttcattc gcaagatcgc cggcttagac gcggtggatg aggcggtagg
    59761 tcgcgctacg gaaatggggc gcaagataat ttacattccc ggaatcaatg acctggatga
    59821 catccaaact atagcaggta taaccatttt gggtcgggtg gcgaaattag tggctgaata
    59881 tgaagctaag cttgaagttc cggttagccg ttctatggtt atggtatcct gtcgtgaaac
    59941 agttaaggaa gcatatatta acgctggtcg cccggatgcc tacagcgatg atatggtgca
    60001 ttacctgacg gatgaccagt ttgcttatgc ggcggcatgc gatggtattt tcgttagaga
    60061 aaaaccggcc gctatatttt tgatgggaac tttttttgct gaatctttgg tcttggctga
    60121 gaccggaaac tcggtgggtg caattcagat tgcaggaact gcccggcctg cacaattgcc
    60181 attctttgtg gcggcttgcg attacacttt aatcggagag gagctttttg ctgcttccgc
    60241 ctatctttcc aaagacccga agcttttggg gagcctgagg gggcaggatg tggggaaagt
    60301 gttgttcttg cttggaattt tggtagggac gatcctgatc tcttttaata ttttggatat
    60361 aagttttatc tttaatgtaa ggtgaattgg gagttagcct aaaatgcgca gagaggtacc
    60421 gatagccata acagcagtag taggcatcat ctttttgatg tcatacttca cgccccattt
    60481 ccccttaaat gtattgcaca actcggcaac tgatatattt atgataatcg ctgccttttc
    60541 catcctctta ggggttctaa atctggtccg ggtgaatctg gagaaaatat ccaaaaagaa
    60601 gagggattgg cccttcagca tagttttgat cttaggtctt ctggttatga ctatcgtggg
    60661 attgggtttc tcgaaaggca gaaatttcat ggagctgggg actaacttcc gctacctgta
    60721 cgattatgtg tataatcccc tatcagccac catgttctcc attctggctt ttttcattgc
    60781 ctctgcctct taccgtgctt ttcgtggacg taacttagag gcaaccctgc ttttggtggc
    60841 ggcttttgta gttatgctgg gaagagtccc aataggttat tatctttcct cctggctacc
    60901 aacaaattat catctgggag atgctgctag ctggattatg aacgttcctc aaaaggcggg
    60961 acaaagagca atcatgattg gcattgcttt aggtgtggtc tcaacttcga tacgtatcat
    61021 cctgggcttg gagcggacct atctgggtgg ggaatagaaa ctattttgga ggaaataagt
    61081 gaactttttt gagaagcttt tggtcattga ccgccgagtt atctttcttt tcgtggctgt
    61141 ggctattgcc gttcccctct ttttcaaagt caatctgaaa gtcgatatca ctcccgaagt
    61201 aaggaaagtt tacgatataa tggagaatct gccggagggg tctaaggtac tagtcgcttt
    61261 cgattacgat cccggctcca tgccggaact tcagcccatg gcggaatcct ttatcaggca
    61321 ctgcataaga aaaaaattga agatgattat tatgggtttg tggccccagg ggtttattca
    61381 ggcgaatcag gctttggaga aggtgctgaa agaagaagaa ttcaaggatg ttaagctaga
    61441 ggaaggtgtg gattgggtca acttgggtta tgctgcaggg aatgaggtgg tcattgagag
    61501 gatgggaagc gatatttcag ctgtttttgc tgcagattat agaggcatga aattaggcaa
    61561 tctgccttta atgaaaggcg tcaaaaactt cagcaatatc gatttcatat ataacctgtc
    61621 cgctggttat ccgggaacac aggagtgggt gcagtttgcg gttgataaat ttggcgctaa
    61681 attagcggct ggaaacactg cggtgcaagc gcccctgatc tacccttttt atcctaaaca
    61741 gcttttgggg gtgttgggtg gtatgaaagg ggcagcagaa tatgaagttc tgatgggaat
    61801 tcctgctacg ggggtaaaat acatgttctc acagtcagtt gcccatggag tggtctgttt
    61861 ctttgttata ataggtaatt tagctttctt tttaaccaga ggtaaaagaa aaagcggagg
    61921 tcaaaagtga gcgaagctat tggactatgg atcggcgcgc ttttgacttt ggggattttt
    61981 agttttctct ataaggacaa ccctctttat aaattttgcg aagccctttt tgtaggaatc
    62041 tctgcggggt attttgtcgt tatttcatat tggcagatca tagttgcaaa aatggtggat
    62101 gtgctcaggg tgggtttcga ttattggata gtcttgagat attttgattt tcagagctgg
    62161 cttccgctgt tgggaggcgt ttttggaatc ttgatgcttt tgcgtctgtt ccccaagatc
    62221 ggttggatat cgcgttggtc attggcttta gtggttggca ccacagccgg cttatatttc
    62281 accgtctact tagtgagcaa tggtatgatg caagtcaaga gcaccataat ccctttgtat
    62341 gtggctgggg atatgagcac aaccatcggc aatatcgtgg tggtcttagg caccgtctgt
    62401 gggctgattt actttttctt ctccaaagag cataaaggcg tttttggcgg agtggctaag
    62461 ataggaatct ggtttattat gattaccttt ggggcctctt ttggttatac tgttatgtcg
    62521 cgtatgtcgc tattgattgg aaggatggat tatttgggga tatcttttgg ggaatttttg
    62581 aagttgctta agtgaaatag aagagttaaa ttataaaaag caatattagt atccccgcct
    62641 tggcctaaaa gagcgggaaa ccttggccca tcttgagggc aagatgggtt tactgcttac
    62701 aaaaagcaaa tgaaaaagag aaggtcatat cctttaattc tattcttgct gttagggttt
    62761 cttttgattt gggttattgc ttttgcccaa gaagaatctc ccttacctcc aagccaggtc
    62821 aaggtcaaag acacacccaa tgacaatggt cattccttaa ccatcacctg gaagtcatca
    62881 ccggacagcg ccaagattct aggttatgag attttgaggt ctgattctcc ggatggtgaa
    62941 tttaaagtgg tgggacaggc attagccaca gataccttat ataaggacaa gggagataag
    63001 gaaaaagaaa gccccaacta tttccccagt cataagaatt tttattacaa agtaagagca
    63061 aaaacggatg gactgtcttc cgagtcagaa atctccttac cagcttatgc tttccagcaa
    63121 tggtatcacg ctgggaaaac cccggtgttg gtagcagtcc tcatcttcag caccttaacc
    63181 ctgatcttca tcagcaaagc caaaaaaggc aaggagcttt atgttagacc tctggctgga
    63241 attggggcag tggatgaggc gattggccgt gcgaccgaaa tgggaagacc tatacttttc
    63301 attttaggct taggaaccgc ggcggatatc gccacgattg ctggttttac catcttgagc
    63361 cgagtagcaa aaagaacagc ggaatataag actagaataa tagttccctg caatgacccc
    63421 attgtgatgg cagttgctca ggaaacagtg aaggcggctt acatggatgc cggcagaccg
    63481 gaggcttatg aagaaaagaa catattcttt ctcacctcca tgcagtttgc ttatgtggct
    63541 ggggtgaatg gaataatgtt aagagaaagg accgccacca acttctatat gggactgttc
    63601 tacgcggaat cgttaatctt ggcagagacc ggctcaattg ccggctcaat tcagatctcg
    63661 gggactgacc agatgtctca acttcctttc tttgtggcgg caaccgacta tactctaatc
    63721 ggcgaagagc tttatgcggc aagcgcctat ctgggtagag agccgttgct tttgggagcg
    63781 ctcaaagcgc aagattgggc caaggggata cttttgattt gtttaatact aggaactatc
    63841 ttcaccagtt tacatttaga tttctttgct aaccttttca aattcagttt ggaataagat
    63901 atgagaagac aacttcccat cttggtagtt ttcatatccg ggattttcat ggcgatacag
    63961 tattttgttc ccagtgagcc ggcagagttt gtgcaggaat acgctatgga ctgggtaata
    64021 gttattggag tatttgccat gggtttggga ttgtggagct tgactaaagt aaactggatc
    64081 aaggttcaaa gacaaacgcc aggttggggt tataatttag tcaccatttt gggaatggtt
    64141 tttatgttgg tggtgggact gatttgggat ccaggattag ggtttttccc gggagaaaag
    64201 tcgcaagtaa gtggaacctg gtttatgaac tttttctggt atatctatct tcccattcag
    64261 acggttatgt tttccctttt agctttttat attgcatcgg cggcttatcg cgctttcagg
    64321 gcaagaacca tcttagcaag tattttactt gttaccgcca tagtgattat gttgcgcctt
    64381 attccattgg gagctgtttc cggcataaac cagtggttgg ctaactggat tctgatagtc
    64441 cccaatatgg cggctaaaag agctatctgg atgggggttg gtttgggaat ggtggctatg
    64501 gccttaaaga tagttttggg tattgaacgt tcttatatgg gcagagatta atttatgggt
    64561 ttctatgaaa aattgatgag tctggaccgc agatgggtct atctatgtgt ggcgattgca
    64621 gtggttttgc cgtttctttt agaagaattc ggggttccga tatttatacc accctatatt
    64681 actccagaag tgaaaagcgt ttacaacttt gtagagaaac taagagaaga tgatgtactt
    64741 tttctggcta tcgattacga tccgatggcc aaggcggagt tgcaccctgt ggctagggca
    64801 gtattaaaac agtgtttcaa aaagaatgtc aaggttatca tttccgctct tagtcaaaat
    64861 ggtccgggaa tggcagagca gcttatatcc cagactgcta aagaatataa caaagttgat
    64921 ggggtagatt atgtttattt gggatacaaa ccctttccgg ccctggtgat tatgggtatg
    64981 agccagaatt ttaagttgtt cttccctaag gactattacg gcaccccgct tgattctttg
    65041 cctatgatgg caaaagtaag aaacatcaaa gacactaagg gagtgattga cgttgcagcg
    65101 ggaagtgtgg cggatatgtg gataaatgaa ggtgccggaa gatacaacta tccattagct
    65161 ttagcagtta ctggagttat ggctgcggac tattatcctt ttttgaattc aggtcaacta
    65221 tttggactgg tgagtggaat gaaaggcgcg gctgaatatg agtatttaat ggagaaaggt
    65281 gggtatatga aaaaagagga gtttgacgct tctcgcggta tgcccatcca gatgtttgcc
    65341 catattgtag tcatactctt cattatcatt ggaaatgtcg ggtattttat gtctagaaga
    65401 aaaaagtctt aaaggggaga taaggcgtga gcttagttga ttttatgtgg atgagcatag
    65461 gtgctctttt gacgcttttc acattctcct atctgtataa agataaccca ttctataaat
    65521 tcgccgagca tctggtagtg ggagtctcag ccggatattt tgcagtgctt ctttattata
    65581 gctcttttat gccaaaggtc atagatacgg tattcaggcc tattgaatgg aaatatttta
    65641 tcctggcttt caaacagata aagccagagt atctattggc agcactcaaa ggtagtcagt
    65701 ggtggtatct tgtcccgggg atattagggg ttatgatgtg gactcgtttc tccagaaaac
    65761 tttcctggat ttctcgcttc tcacttgcct tctacatggg cataggaatt ggtgcctcca
    65821 tacccttgac tttgcagaac tatgtgttga agcatttgac ttcgactata cagccggtca
    65881 atttcaccag cttaaccgga atatggcggc ttttgatagt gattcttgta ttttgtacct
    65941 tggtttattt ctttttctct cgcgagcaca aaggggcatt cggagcttcc gccaaagtcg
    66001 gaatatgggt tctaatgatt gcttttggag cgggatttgg tttaacggtt atgggcaggg
    66061 tggcgcttct ggcggagagg gttattttcc tgagggatta ttgggttgag ttgaaatatt
    66121 tcctttatat gcttttcaaa tacgggatgc tgctttaacc ccaacccctt ataaatttga
    66181 tctctaaaat tatcttctat ttatcattct ggtttgattt caatcgggtc aatgtcttag
    66241 ttacacttct ggtcttcttt tccttcctga tttattttat cggaagagcc aaaaaaggga
    66301 aatccctttt tattcgtcgc attgccggct tggatgctat cgaggaggca gttggccgcg
    66361 ccaccgagat gggaaggccc gtactttata tccccggaat agaagatatg gataacattc
    66421 aaaccgtagc cagcatgata atcttaggta acgttgccaa gatggcagcg cgctatgaga
    66481 ctaccttgat tgttcccatc tgtaggccgt ttgtggtgcc ggtggcagag gaatcagtgc
    66541 agcagggcta catggctgct ggccgtccgg atgcttacga taagaacagt gtgcgttata
    66601 tctcagatga gcagttcgcc tatactgccg gggtgaatgg gataatgcta cgggataaac
    66661 cagccgcaaa tctcttgttc gggtccttct tcgctgagtc attgattatg gctgaaaccg
    66721 gcttttccac cggagcgatt caggtagcag gcacagctaa tattcatcaa cttcctttct
    66781 ttgtggtagc ttgcgattac accataattg gagaggagct ttatgcggtc tcggcttatc
    66841 tttccaagga gccgaaactc ttgggaacac ttaaaggttc tgatttggca aagcttatta
    66901 taatgataat actcattttg ggcgcgattt tagccacagc taatgtcgag tggtttagaa
    66961 atctgttttt tgtgcagtaa ttttgtatgt agcagggttg gccaagaagt ctattgtcat
    67021 cgcgagtgac cagggggagc gtggcgatct cgatttcata atccatgatg ttataagaga
    67081 ttgccacgtc gtccgccgca ggccctcggc ttccctcggc ttcgccgaga ggcgagcgcc
    67141 gagaggcgag cgagactcct cgcaatgaca gccgaggaat gtttgagtga attaaaagac
    67201 ccttaagata ttgacaaaat gggtgtcatt ctgagtaaag cgaagaatct ctagtctttg
    67261 atttatgata aggcgagatg gattcgcttc gctcaccatg acaatccccc gcgcttttca
    67321 acatatccac tagaggaaaa ggattttatg cgacgcgaag ttccactggt aatatgcttc
    67381 ctttttggaa ctctttttat tgcggactac tttatctcca atcctggttt tgatagcctg
    67441 gctcatacca tcaatcagtg ggctataatt gtgatgattt ttgcctatgt tttgggagtg
    67501 gctaatatcg ctcgcataaa cagcggcgtg ataaaacgaa aggagaaaga ctgggcatat
    67561 aagttagtcc tgctgtttgg gcttttattt atggcaatct tgggaattgg ctggggagtg
    67621 actgagggaa cagtctttta ctgggtttat atgaacgcct attacccaat gcaggcaact
    67681 atgtttgcct tattggcttt cttcatcgct tctgcggctt tcagggcttt tcgaattaga
    67741 agctgggaag caggactgtt ggctataacc gcaatagcgg tgatgattgg cagggttccg
    67801 gttggcgagg ctgtttggaa aaactttccg aatttcactg aatggataat ggatgcgcca
    67861 caattggcgg ccaaaagggc gataatgata ggtgcggctt tgggtgctat ttcaaccggc
    67921 ctgaaagtcc taactgggct ggaaagaacg tatcttggcg gagactaggc agacgtctgg
    67981 ggaaggaggt tgtcatcatg gcactattcc acaggatctt gatagaggca gatcttaaga
    68041 tgtctcaaga cccattggtt agtgtagaat tggtacactc caactctttc cagttcgaat
    68101 ttggtcaaat ctcccctgac cctcgcctcg attcggcccg cctcggcgag ccatcggcga
    68161 gacgggcgaa gcgggcctct ttagtaaaga ggggaagaaa gtgtcccccc ctttgaaaaa
    68221 gggggaacaa gggggatttt gaaataattg cacaatgacc aacgtgtaac tcagaatgac
    68281 aaatcggcct aagattatag agagaacaat acagcaatga aagagaaact gataaagata
    68341 tttgaggcat tagagaagat ggaccggcgg ataatcttta tctttatcgg cttggcagtt
    68401 attctcccat ttttcattcc gttcggtttc gagttctcag tttctccgcc cgtgcaaaaa
    68461 ttctatgatt ccattgggtc aatccccccc ggctcaaagg ttctcctctc agctgattac
    68521 gaccccgctt ccatgcctga gctttatcct atgcatttgg cagctttgaa gcaacttttt
    68581 tccagagact taaaagtcat ctgcacacag ctctggccta ctggttcacc tttggcagag
    68641 atggcttttc aagagatcgc agttggggag ttcgacaaga aatacggagt ggactatgtc
    68701 aatctgggtt tcaaggaggg aagagaggtg gtgatggtct caatgggaga gagcattccc
    68761 aagacttttc ccacagatta tcacggaacc aaagtcgaag acctgcccat tatgcaaggg
    68821 gttaagaact tcaacgatgt ggcaatgata gtgaatatct cagccggtgt tcccggaaca
    68881 aaggaatggg tgttgcaagt gcagtctcgt tatcatataa agttggcttc tggtactact
    68941 gccgtttccg ctccggagtt ttatccttac cttcaatcta atcaattggc tgggctttta
    69001 ggagggatga agggtgcggc tgaatacgag aagctggttg gtcggattgg attagggaga
    69061 agggggatgg attcccaatc ggtctcgcac atcgtgatat tcatattcat cctcatcgga
    69121 aatattgcct atttttcaat ggggggaagg aagaagaaat gattcacaca gagataatcg
    69181 gaacttggtt aggtgcggct ctgactttgt ttatcttttc cttcctatat aaggataacc
    69241 ccttttacaa gttcgccgag catctatttg tgggagtctc agccgggtat ggggtggcta
    69301 tggagtttca caatgtcttc gttcccaatt tatggaagcc gctttggggt ggaaatctgt
    69361 ggtttttggt tccctttgct ttcgggattt tgctttttac ccgatttttt tcaaagattg
    69421 cctggctttc ccgttggtca atcgcattga ttattggtat attcgccgga atctatattg
    69481 tgggttacgc ccagggagat ctggtacagc aaatacatgc taatctactt cccctctggc
    69541 aggggaatct cttcagcttc atccgcaact ttttatccgc tttctataat tggttaactt
    69601 tcaggggaag tgtactgatt tggttagact acaccaagaa cttcctttta gtggtcggag
    69661 ttctcgtctc gttggtctac ttctttttct ccaaggagca taaaggagca ctgggagtgg
    69721 gcgccaaagt cggtgtttgg tttttgatgg tatcattcgg agcttctttc ggctatacgg
    69781 ttatgtcccg tatgtccctt ctaattggca gagtatattt ccttctgggt aattggctgg
    69841 ggttaatccg ctaagtaaaa acccccgcct tccggcgggg gttttttatt aatgttgtca
    69901 tctgtccatt gcctatgtcg agtcaggagt gggctgaata agatttattc ttccggataa
    69961 tatgatgcct cctcgcgcat cactagtatc cgctactgtt atattgaccg cctttctcga
    70021 gacagtgctg tcatagaccg gggtagtgaa aatgaagttg gctaatccat aagaatcagt
    70081 tatatcgatg tctgagccgc tgattgtgcc attggatata tttatcacgt tgattcgatg
    70141 accgcccaag ggattcttga agacatccgt tatccttaca gtaaaactac ctaagctatt
    70201 gaaatctatt tcgttatcag cgatgacacg agaatagttg ctcgtctcat ccttatagct
    70261 tgagccggtg gtaagtggga aagtaacagt acctacagcc cctccacatc ctgcccaaac
    70321 ccactcattt gcaccaattc cattgtccgg ggtggaccaa gcgtaatcaa cgtccaggtt
    70381 tatactagta tatttacttg atgcaaaggc ataatctcca ggtatgcagc tgctgtcagt
    70441 tttggaatcc cctatggttc cgtaggtcgc ttcaaaatct accttgtatc ctgctaacac
    70501 agggtttcta ttgatatcct ggacccccgc aaaaattgtg taggaggcct tggatgtggc
    70561 gggtatgccg gaagagggat agctaagaaa gtcaacgtag taaggcgctc cggaaactat
    70621 tagaatgacg ctatctttga tagtgcctcc agctgtttcc actcgaatcc aggcaattcc
    70681 agtatctcca ctagtaggag ctgaacgata ggtggctgaa gccactcctt cctcagtttc
    70741 atcatttggc gtaatcattc cctcgtccgt gtagaaatag actgaagttc cgtttggaac
    70801 atagtttcca aacgaatcga ccactactgc tccaacttca gcttcggcat tcacaatgtc
    70861 aaatccccta atgttgcaag gacgcactcc tacagagatg aattttggcg ggccagcaca
    70921 gacagttatg cgggttgaat ttgatatcac attgccaaaa taggcaatca agtttatcgt
    70981 tccagattta gttccactat taactgtaac ttgagctatg ccagaggagt tggtggatac
    71041 tgtgcaggcg ctagtgcttc cgtttatgcc ttcccctcca tccggtccat ttaggatcct
    71101 gaatacaacc ggccatcctt cgactacttg atttccgttc tcatcataac aggtggcggt
    71161 catctgcaca aactctattc caccagttcc cttgacttga atggttggac tggctggctc
    71221 tagagcgata ctggcaatac tatctccggt tgtgggaatt agaagaatcg tggcatcgtc
    71281 ggttaccggg tcatccggtg agccagcagt tgctttaata taaaccattc cagctctgaa
    71341 tccagtagta taagtcgcag ttgccactcc ggaagtggta gcggcggtca taggtatatt
    71401 tccaattgaa ttccagacac cgtcattgtt ggtatcgtaa atcagttggt caatgtcttt
    71461 gttatagtag ccatccccgt caacatcctt gaatttctct ccagccgcta atctaactat
    71521 ggttccatct gtaactggaa ggccattagc atcccgcaca gttatggtca cttcgctggt
    71581 tgtatcccca ttagatggaa gaactgttgg gtcaacttca attgttaagc caccttcgcc
    71641 gggagtgaca cctgaggaga taacgcgaac ccggatgctg gcagtagtat ctgaagttga
    71701 ggcgtaaaca taagctgtgc cagtattgtt gccatggaaa gaagtaacaa ccagaccatg
    71761 ctcttcgctg gtatcaacag tggctgaaag agttccaaca tagctgggtg agacagagaa
    71821 atttactatc tctccagtat cgatctgccc accagtggta actgttgcat aaatagagat
    71881 actgccaccg actagtgttg agtcagcagg actggcgctg agattggtca caattgtttg
    71941 agccgctccg ctccctgcag tcgggctctt gtctttggcg caactaaaaa gcaaagccac
    72001 ggaagccaag aacatcaaaa acaaaacagt tgcttttaga aaagaatttc ttttcataat
    72061 agctcccttt cttgatttag aagatttact gtttcctaaa ttattttatc ggcaattaaa
    72121 agatttaatt tagcccgcaa aaatttaaaa aactgtaaaa gtttggttgt attttgaggt
    72181 tttctctttt ttcttatagc acagggagtg ttatgaagaa aatcgtttca gtttttcttg
    72241 tgctcttgtt gtcagtcggc tcgtcttttg cccggggaga cacccttgat tttcgtgtga
    72301 agactgatgc gggcaagagc aaacaaaatt accctgcaat tgcggccagc ggtcagggga
    72361 gaacagttgc ggtttggcag gattatcgtt cgggaaataa ctacgacatt tatgttcagc
    72421 tttttgactc ctccggcata aaaatagagg ggaaagttcg tctttaataa ctttgggtca
    72481 ttcctaaata agttgcaggg atttatataa gagattgaat gataaatcgt tcgacaagct
    72541 cccttcgggt ctgagagaca gcaggggcgt atagcagccc atacccccta cccaaaacct
    72601 taatcagtag cgaagtgtcg ggtagatgaa agcgaagtgt cgggtcacaa gactgcccaa
    72661 tctcaagaca catctcaaga cccagaattc ggggaagtcg tggaattcgt gtctcaagat
    72721 gcaggattcg cacaatgatg aatgctacgg gtcctagatc tgcactttaa atacgaaaga
    72781 acgaaaagtt cagattctga ttgatcaaga gatgaatagg ggtgccgctc agtctcttgg
    72841 gatggaaagg atcataacag ccagccaata agcagtggaa tttacttctg cgagttaaaa
    72901 agtgagcgat tcaggtcgat aaagaagatg actcttgtga agtaaagaat aggctgatag
    72961 gaggatttgt gaaaagaata atcttctggc tatttttgtc tttctttatt ttttctgttt
    73021 cctttgcagt tgagttcaaa gttagcaaag aatatgaaga gaccctgatt cgacaggtag
    73081 aagaacttta tactaaccag gaaccaatct acccccttcc caaatgcggc actccaataa
    73141 tggctgagat ttctctgctt aaggagaaac taagctctga agcattggag ttgctcaaat
    73201 tttacttagc cagaccgagt ttgcagagaa gttataacac cccgggcggg catttcaaaa
    73261 tccattatga cattactgga acaaatacag tctatcaccc atccgaagac acaaatcctg
    73321 ctaatgggat tccggattat gttgacagat gtgcggagat ttttgattac gtctgggcta
    73381 aggaaataga tactatggga tataaagccc cgcccagtga cggctctatg ggtggggata
    73441 gtcgatacga tatctatata attaatttag gatatgggta tttaggcggc acctctcctg
    73501 aatcaccacg tactccttcc tggagttaca ccagttacat agagctgacc aacgactttt
    73561 ctggttatgg atatgcaagt cagtatgacc tgatgagcgt aactgctgga catgagttct
    73621 ttcatgccat tcaatttgga tatgatgtat atgaaggtta tgacccctat cccaattgga
    73681 agccctattg gatggagatg tcttcaacct ggatggaaga ccaaaccttc gataatgtga
    73741 acgactaccc taattacctg cggtttttct atcgttatcc ttgggtatcg ctgaaagcct
    73801 tttccaatga ttttggagcc gattcggtta gggcatatca tccttatgct tcgtgcgttt
    73861 tcgccattta tctctcggaa aaatttggag ttggcgtaat gaaagatatc tgggaagaat
    73921 gtggaagggt gccagacgcc aacactctcc ctgctattga tagtgcctta gctccctttg
    73981 gcgctgattt cgacagcgcc ttcaaggaat tcttagtttg gaatctcttt accggcacga
    74041 gggcaaacac ggttaactat tactcagagg ggaatttgtt tccggagatt aacatcttaa
    74101 atgagcaaaa acacagcact tatccggtaa atgtaacttc agttgattct atgccagaga
    74161 acttggcttc gaattatgtt agattcaccc cactttctgg tccaggggga ctggatatga
    74221 cctttaatgg agctgacaat gcagaatggg ttgtgccggt ggttgggtac aaatcaggtg
    74281 gtggaaattc tattggcgcc tttagtttga atgcccaagg ggaaggaaat tttgactttt
    74341 acaactggga tgattacgat tatgtttaca tgacacctgg ggtcctaacc agggattcga
    74401 gcaagtacaa ttacgcatac gctgatactt tcgatatcaa tttgggagta gatgaagacc
    74461 agcttccaga tagaatggaa tatatcttac cccaaaacta tcccaatccc ttcaactctt
    74521 ccaccaatat acccttcacc gtttatggtt cacagttcac agttgacagc cgcatccata
    74581 ccaccctcaa aatctataac attttaggcc aagaggtgag gactttagtg gatgaaccaa
    74641 aaagaccagg aaactacgag ataacctggg atggaaagga taatgaaaac agagatttac
    74701 caagtggagt atatttctac aagatagaaa cagaagggtt tgttcagtcg aagaagatgt
    74761 tgctcttgaa gtagattagt tcaattggag tagtttcttg gtattcagtt tgagctggtc
    74821 caagttggag gtcttgacca aataggcatc tgctacccag gaggagaaat cagacttgaa
    74881 ggtctcgtag gcggagttga gaataaccgg aatatcttta ttaaattgct ttatctcgtg
    74941 caagactttt aaaccatcca tttttcttat ctttatatcc agtataacca ggtcgggaag
    75001 ctgttttttg accaagtcca aggcaacttc gccatccata gcagttaaga cctcgtaacc
    75061 ctcatcccgc atctcctttg tgtaaagctc caggaggttg ggctcatctt ccactattag
    75121 tattttaggc ataacttcct tttaactttt ttggttcaag gggagatgta tggtaaatct
    75181 tgttccctcg ttgggcttgc tctggacttc aataaatccc gcatggtcat ccacgatttt
    75241 tttggaaact gccaagccca aaccggagcc agtgggcttg gtgctgtaga aaggtataaa
    75301 cagctttttc atctcgttct ggttgatgcc acttcccgta tcctctattt ctaatacggc
    75361 ataattatcc tttttcttgg tgcggatggt taaagtcccc cctccgaaca tagcgtctat
    75421 ggcgttctca atcaggttga aaagcacctg tttgatgcgg tgggaatcaa gatctgtctc
    75481 cttgaggtca tcggagagaa cagtctgcag taatatattg tcatatttga actttgattc
    75541 cagcacctcc aaggcttctt ggacaatacg attcaggtcg tttctctgaa tagagagctt
    75601 actttcacgg gagtaatcca gaatctgaga ggttatctcc tccaagcgtt ccacctcttt
    75661 gatgataatt tccagatcgc gaaggtcgag cttgccgctg gtgtagtttt tcaggacgcg
    75721 tcgcgcatat ccaccaatag cgataagcgg ggtcttgatc tcatgggcgg cagtggtggc
    75781 taatttaccg atatctgcca gacgttcatg tttcaataga taactctggt tttccctcaa
    75841 aagctggttg atatgctcca gttcctggta tttcaatctc agttcccggt gaagtagcat
    75901 attctcaatc tcagacccgg cttggttggc aaaagtctcc aaggcaaaca gatcctcatc
    75961 caatatcggc tcagcggtaa tgaggttgtc agccaccaga acgccgagcg gcttaccctc
    76021 agatagaatc ggcaccacga ctattccaca tgggcttaat atttgggcta acttattatt
    76081 acccacttga ttgggtggga aactctttag gttcaaggat ttcttatcgg agagggccca
    76141 agcgagaata ttgtcctgtg aatcgaggga taaagctatc ttttttgttt tcttgatcaa
    76201 ctcgttttca ggtttacctt cccctttgag gatattatgg ataatctggt cgaaagttat
    76261 gtttttactt tttaactctt cccaaatttt ctccgcctcg ttgttgtcct gtggccccag
    76321 tgccatcttc ccctctagga catttctctt ttcattaatc aggaagatga aggcgcgatt
    76381 gaatccaaaa gcttggccag ctgtggcacc gcttagaatc aagagaagcg atttatccaa
    76441 atcatctgat ttcaaaagag catccgaaac cttctttagg acgttcaact cctccagcct
    76501 tttccttaaa ctcccatccg tgctcttgag ctggtcatta taagaatcca gctgagaaaa
    76561 caggaatttc gtaaaataat tagaagtctg gtccgcctgc cttctgcctt tagctctcaa
    76621 agacagttga ttgaaggtct ggcaatccag gcagttcttg agattcttat ccaatgggag
    76681 ttctgatagg tcagcttgct ggatggctaa tctaagccag caggtggtat tggaactata
    76741 aggggtaggg cagtgaaatt ggtgttttga ttcttccaca tcgtcccccg gatgggtttg
    76801 gtcataatct attacatatt acgttaaaat agtgaaggtg tcaacacttt ttgtgttgca
    76861 aatttagact tcagatgtta atttcaaaac atgaattggc aggacagaaa atttaaagct
    76921 ttaatagaaa gagctctggc cgaggatgtg ggcactgggg atataacatc tgaggcagtt
    76981 attgataaaa gtttaatgaa ttgcgccaag ataaaggtaa agcaaagtgg ggttttggct
    77041 gggattttct tggcggaaca ggtctttaag actgttgacc gaaaaatcaa attcaaactt
    77101 tacaagaagg acggctcgca gataaaaaag ggagaaacca tagcgttggt ttgcggtaag
    77161 acgagaagca tactgcaagc ggaaagagta gctttgaact tcttgcagaa attatgcgga
    77221 attgccactt tgacaaatga gtttgtaaag aaagtaaaaa aaaccaaagt taagattctg
    77281 gataccagaa aaaccactcc aactttgagg cttttggaaa aatatgcagt caggatgggt
    77341 ggagggaaaa atcaccgctt tggccttcat gatatgatat taataaaaga caatcatatc
    77401 cgagcatgtg gcagcgttaa acaagcggtc ttgagggcaa aatcctttat agctcaatcc
    77461 aaacccaaaa ggaaaataaa aatagaggtg gaagtaagaa atataagaga attggaggag
    77521 gcaataaaat caggggtgga tagagtgatg ttggacaata tgcatactgg tgatatcaga
    77581 aaatgcgtta agctagtgcg ttcctccaag aaaaaaatag aaatagaagt ctcaggaaac
    77641 gtcgatttga acaatgtaat aaagatcgcc agaacaggag tcgatttcat ctccatcgga
    77701 gctttgaccc attccgcttt ggcactggat ttgaatctgg tcttatgcta atacaaatgg
    77761 attaatttac gttcaataaa tctcccctcg ccctcgcctc gattcggccc gcctcggcga
    77821 gccatcggcg agacgggcga agcgggtctc ttttccaaag aggggaaaaa agaggtctcc
    77881 ccctttgaaa aagggggatt atatgtaaca acatttgatg cgtttgccat aacatggttg
    77941 atttactctc caataatgct gaaaaagtcc tgaaggtatt aaagagaaag aaaggcaggt
    78001 ttttggccac ccggaagatc gccgcttctg ccggactttc cagttttaac gtctatcaat
    78061 cgatttacca cctacaaagc tgggaatacc aaatagaatc acaaaagggt aaaggttatc
    78121 gtttggtaaa acccactgac cttttacttc cttttgagat aaaggataat cttcatacca
    78181 agtttttagg tcaaaagatt cattcctatc aaaccctcaa atcaactaac ctgttggctt
    78241 ataagttggc ggaagcgggc tctcctgaag gggctattgt catctcagaa aagcagaccg
    78301 cgggaaaagg aaggatggga cgagaatggt tttctccatc caaagtgggt ttatggatga
    78361 gtctggtttt gaggccggac attccaccct ccaaagcagc agggctttcg ctttgtgctg
    78421 ggctggcttt ggctcaaacg attcaaaatt tgtttggctt gaaagcaaag ataaaatggc
    78481 ccaatgactg tctaattaat ggaaagaaag tagcgggaat ccttctggaa ctttccgctg
    78541 aaattgacca gattaggttt gtgataatgg gggtgggaat aaatatcaat caaaagagaa
    78601 gcgattttcc ttcgggctta caggataaag ccacatccat cagaattgag ttgggccaaa
    78661 gagtcaggcg tttagatact ttgaagtctt ttttggagaa gtttgaaaag atatatctgg
    78721 tattcagaaa gaaaggcttg ggaacttatc gccaggatat taaaaaatac tcttcggtct
    78781 taaacaagga aattaggatt aagtctggag acgaagtcat aaccgggaaa gcatatgaca
    78841 tagacgataa tgggtctctg gtaattaaga ctaaagatgg aactagggcc atcataaccg
    78901 gagaggtgaa cttactgtga tttgtgtctc aacacgcaga tctgcgtaca gacgcggtgt
    78961 gggatatggt tcaacagttc ggcgagctca ctgtcctgag caaagtcgaa ggacaagttc
    79021 accatcctga gcaaggtcga aggaaattcc cgtcagtaga gacgcaacat tttgcgtctc
    79081 tacaaatcac agggaatgca ttcctgtgcg ggccgcaaat ttatcgcgag gcgacccgta
    79141 gggtcgccta cacggttcga cttcgctcac cgtcctgagc aaagtcgaag gacataattc
    79201 gtggagaaaa aaatggatga aagcatattc aaatcttatg atataagagg aatttacccg
    79261 caacagttga atgaggatat agcttacaaa ctgggtcggg ctctggtgga gtacttaagg
    79321 cctcagactg tagctgtggg aagagatgtg cgtctctcct cggagagctt agcttgcgct
    79381 acaatcaaag gaataactga agagggggca gaggtaattg atatcggatt gtgtagcacg
    79441 gatgggctct attttgcagt tggaaatttc aaatacgatg gtggggtgat gatcaccgcc
    79501 tcgcacaatc ctccggaata taatggtatg aaggtctgta agaaagatgc ggttcccctg
    79561 agtgggcaag aaggattaga caaaataaag gagttggttc tagggaacag ttttcctaca
    79621 gttaagaaaa caggcaaaat aacaagacgc gatattgatc aagattacac ttcgcatacc
    79681 ttgtcctttg ttgataaggg ggaattaaag ccctttaaga tagtaatcga tgcaggcaat
    79741 gggatagctg gaaagatcgt tcctcaagtt ttcagggaat taccctgtaa ggtaattccc
    79801 ttattctttg atttagatgg gactttcccc aatcacttgc ccaatccgat cgtgccggag
    79861 aatacggttt ctttgagaga tgagatcttg aaacaaaaag ctgacttggg ggctatcttt
    79921 gatggagatg ctgacaggat gttcttaatg gatgaaaagg gaaaactctt gggtggagat
    79981 atggttactg ccctggtggc taagaatcta ttaaaaaaag aaaaaggggc taccattctt
    80041 tataatctgg tctgctccaa aactgtgcca gagataatcc agaaaagtgg aggaaaagga
    80101 attcgcacca gggttggcca tgctctgata aaacctctga tgaaaaagta caatgccatt
    80161 tttggaggag agcattcagg tcatttttat tttcgcaaca actggttcgc cgactcgggg
    80221 ttaattgccc ttttggtctg tttggaattg atatccgagg agaataaacc tctttccacc
    80281 ctattggcgt caatcgacca ctacttccgt tcaggcgaga taaatagtca agtagccgat
    80341 attccgggta agttaaaaga agttgaaaag agatatgctg gttcgggaaa aattgaccgt
    80401 ctggatggct taaccatcga gtttgacaca tggtggttta gcctgagagc ctccaatacc
    80461 gagccgctct tgagattgaa tatcgaagcc accagcgaag agctgttggg gcaaaagaaa
    80521 aaagagatgt tggagctgat aagaagttaa gccttaacag ataaccctgc ttgaagtagc
    80581 ccgacttgca ttttgggcta tttttttatt tttctcaagt aagttggaaa tctttcgata
    80641 aaaggaatag aggagaaacg taacctaata agccacgagt tgctaaagaa acggttgagc
    80701 atgaataagc ccattcgagt tttagtggta gatgatgatg ctaaaataag agaacttcta
    80761 ctggataccc tctctgcttt ggggtacgaa gctttggggg caagggatgg ggaggaggcg
    80821 ctaagtctgc ttgaagatga gaaaatagac ctggtagtaa cagatatcaa aatgcccaaa
    80881 ctcagcggag tggcattgct tcaagagatc aaggaaaaga atcctgagct tccggtggtg
    80941 ctgattaccg gttacaattc catctatcct atggaggaga tgataaacca gggggcaagt
    81001 gggttcatct ccaagccctt ccgcatcggg aggattgaag agttaatagt gaaagtcttg
    81061 aatgccaaac cagaaaaagg cggagcaaaa ggcagtccaa ccaagaagat cttggtggtg
    81121 gatgatgatg aggcgttacg tagcgttttg gaggagaccc tggtcagttt tggttacaag
    81181 gttacaactg cttcggatgg ccaggaggca ctggagaagc tgaaagagga aagctacgat
    81241 ttggtggtaa ctgatatcaa gatgccccga atgaacggaa atgaacttct gaaggaaatc
    81301 aaacaggctt ctcctggcat tccggtggtg atcataacag cttatccggc tgcctattct
    81361 cagcaatgga ttatggaaga gggggcagat ggttacctca ccaaaccttt tcacatcgac
    81421 agaatagata agttgattcg cgaattgacc ttagagagaa caccatccta agtaaatcca
    81481 tcctctattt ttttaacaag agtattccca ctttttttca agacacatca gaatgtctta
    81541 atggctattt cctttggttt ctctctcccc ttgggggaga gagaaagagc gagggggcta
    81601 gtcctctttt tttggtctct gaaaaagtgc agccgacagg tcgggcaagg tgcccggcct
    81661 acggaaaacc gataacttac gtttccgtag gtgtggacca tcctggttca caccgtaggc
    81721 ggggctccca gccccgctaa aggacttttt cagaggtctc tttttaatcc ttgacaatcc
    81781 agatttttgt tctaatttta gattacatta gatggaaatc aaagataaag gacttgaatc
    81841 cattcgaggc gacccagacg ggtcgccttc ctttttagtt gactttaagg cataagtttt
    81901 gtaatataaa aaattgagat gaaaaaaata aaagtggcag gaatcattcc agccaggttt
    81961 gcttccgcca ggatgccggg caagccctta aggtcggtgt ggggagaacc gctcattcaa
    82021 agagtctatc agcaggcaag aaagtccgca tttatcgata agcttctggt agctacagat
    82081 gaccatcgta taatggatgc ggtttttggt tttggagggg aagcggttct aacctctgct
    82141 aaacacaaaa gcggaacaga tagagcaggt gaggcggcag ataaactgaa ttgcgacctg
    82201 gtggtgaata tccaatgcga ccagccattt ttgaatccaa agatgctgga ccagcttatt
    82261 tcctatatgt tgaagaataa aaaaatctat atggctacct tagcccagag gataaatcaa
    82321 aaaaaggatt tatctgaccc taatgtggtg aaagtggtaa tggatagaaa tggatacgca
    82381 ctttatttct cgcgttctct cttaccatat tccaaagctg aaaaccggac ttactacaag
    82441 catgcaggca tatacggttt caggaaagat ttcttagcaa agtacgctaa actggcacag
    82501 acccctttgg aaaagtctga aaaactggag caattgaggg ttctggaaaa tggatacaaa
    82561 ataaaagttc taatatccaa atataattca ctttcgattg ataccccatt acagcttaaa
    82621 gagataaatc ataagtcctc gacttcgtcg agagacgagt ctgctttgaa aaggagaagg
    82681 aattgaaaga caaaaagaca aaatatatct ttattaccgg tggagtggtc tcctctttgg
    82741 gcaaaggtat cgcttccgcc tccatcgggt atctgttaaa gcaaaggggg ctgaaagtca
    82801 atctactcaa gctcgaccct tatcttaatg ttgacccggg cacaatgaac ccatttcaac
    82861 acggagaggt ttttgttctg gatgacgggg cggagactga tttggattta ggacattacg
    82921 agagattcat tgacgaaagt ttaaccaaag acaacaatgt tacctccggg caaatctacg
    82981 attcggtcat agcgcatgag agaagagggg attatctggg tggaacagtg caggtcattc
    83041 cccatatcac caacgagata aaaagccgca tccgcaaagt gggagaaatg aacggaaagg
    83101 cggacgtggt tatggcggag ataggtggga ctgtgggcga catagagggg ctgccttttt
    83161 tggaatcaat tagacagatg gggctagagg atggaagaca caatacctta tacattcatc
    83221 taaccttggt tcccttcatt aagtccgcag gtgaacttaa gaccaaacca acccagcaca
    83281 gcgtcaagga gctaagagaa atcggaatcc aaccggatat tttactttgt cgaacctcac
    83341 ttcccttaac caaagggata aaagagaaaa taggtttgtt ctgtaacgtc ccctctgaag
    83401 cggtaattca ggcaattgat ttggattgta tttacgaagt gcccatttcg tttcatcagg
    83461 aagggttgga tgaactggtg ataaagcatt tggatttaga atgcaaggag ctggacctat
    83521 tcgattggaa aaaaatgctg gagaagataa aaaacccaaa ggataaagtg aatatcggga
    83581 tatgtggcaa atatactaat ctaaaagatg cctataagag catcattgaa gcgttcgtgc
    83641 atgcaggagt ggagaataat gctgaagttg aattgaaatg gattgagtcg gataatataa
    83701 taaaagaggg gaaagaaaaa tacttagagg gcttagatgg acttttagtt ccaggtggtt
    83761 ttggagatag gggaattgag ggaaaaattg aagcaattaa gtatgtccgg gagaagggga
    83821 ttcctttctt tggaatctgc ctcggaatgc agtgtgctac cattgaattt gcccgcaatg
    83881 tatgcaactt aaaagatacc catagttccg aattcgattc ccaaaccaaa aatccggtta
    83941 tccatcttat gccagagcag gaaaagattg ccaataaggg tggaacaatg cggcttgggg
    84001 cttacccttg tgttttggaa aaaaatagct tagcccataa aatttatgga gaagatcaaa
    84061 tcagcgaaag acatcgccac cgctatgagc tcaataacga ttacagagaa aagctcacta
    84121 gtgcagggct taaactttct ggtttgtctc cggataaaag attggtggag ataattgagc
    84181 ttccagctca tccttggttt ttaggcgtgc agtttcatcc cgaactcaaa tcccgcccca
    84241 ccagggcaca tccgattttc aggaagtttg tgggggcggc agtgaaatat aaaaaattga
    84301 aaaataaaaa ttaaaaaaga aaagttgatg ccagaaatag cgcttcgaca atataatctc
    84361 aaaatcggca aaaataatcc attagtctta attgccgggc cgtgtgtgat tgagtcagaa
    84421 aagttggttt taaagactgc agaggaaatc aagaggataa cggaaaagca taaaattcct
    84481 ttcattttta aatcctccta ttctaaagcc aatcgcctgg atatttcatc ttttgccggt
    84541 ccgggattgg ataagggttt ggaaattctg aaaaaagtca aagataaagt cggagttccg
    84601 attctaactg atattcatca gccagacgaa gctgtgcctg tttcccaaat agcagatata
    84661 attcaaatcc cagcatttct ttgtcgtcaa actgatttaa ttttagccgc agctaaaacc
    84721 aaaaagccgg tgaatataaa aaagggacag tttatggcac cggaggatat gaagcagatt
    84781 gccgacaaag tcgtttcaac tggaaataaa aatatcctca tcaccgagag aggaacaact
    84841 ttcggttatc ataacttagt agtggatatg aggtctctgg taatcttacg caatttggga
    84901 tatccggtaa ttttcgacgc cacgcacagc ttgcaacttc ccggacaaga agggaaatct
    84961 tccggaggcc agccggagtt cattttacct atggcaagag cagcagtggc ttgtggcatt
    85021 gatgctttat ttgtagaaac tcatccggat gttgaaaagg cattatgcga tggcaaaaat
    85081 atgcttcctt tagaaaagtt agaaaatcta atagttcagg tttgggaaat agataaactc
    85141 ataaaacaat ttgaaaaata agactctttt ttctaaattc aagaatataa aactcatcgt
    85201 ctcggatgtg gatggggtct taacggataa taccatttat atcgatgata atggcacaga
    85261 atccaaaaag ttctccattg cagatggagt aggggcttat ttggcaaaaa aatttggact
    85321 ggaaatagcc ctaatctctg cgagaaaatc aaaggcaact gagattcggg caaaacaatt
    85381 gaagataaag tatgtttttt tgggtaggga agataagctc aggatttacg agaagattaa
    85441 aaaagatttg aaattaaggg atagccaaat cgcttacctt ggggatgata tagttgatat
    85501 tccggttcta aagaaagcag gaatccctat ctgcgttaag gatgcttcga ctcttataaa
    85561 aaagtatgca gattacatca ccaaagcaga gggtggaaga ggcgccttgc gcgaagtggt
    85621 agaaatggtc cttttaagca agaataaaaa tttgacggag attttcaaat gagtttaaaa
    85681 aaagccagag aggttatccg taaggaagcc aaagccatag cttccttaga gggaaaactg
    85741 actaaggagt tctctcgagc agtggatttg attttaaatt gccggggaag agtgattgta
    85801 accggaatgg gtaaatccgg cattatctgc aaaaagattg ccgcaacttt gacctccact
    85861 ggcatttcag ccatatttct tcacccggct gaagggattc atggcgattt aggattagtt
    85921 caaaaaagcg acattgtcat agccatcacc aaaagtggcg aaacagatga actttatcag
    85981 ttaattcctc tttttaagag attgggcgtg cccatcataa ctcttactgg aaatacaaaa
    86041 tcacctgtgg cagagaaaag cgatgtggtc attgacgtat ccgtggatga ggaggcttgc
    86101 cccaataatc taatccctac ctccagcacc actgccgcct tagtgatggg agatgccctg
    86161 gctatggcgc ttctggatag aagaggtttc agcctagacg attttgccct tcttcatcct
    86221 ggaggaaatt taggcaagag attgcttctg aaggtaagtg atattatgca caccgggaat
    86281 aaggtgccga ttgtaaatca aggggcaaat atgaaagagg ttgtcttgga gatgacctcc
    86341 aagaggttcg gcgccaccac agtggttaat agcaaaaaag aattggtagg gatattcacg
    86401 gatggcgacc ttagaaggtt gatagagaaa tcggagaaca tatttaaatt caaagccaag
    86461 gaagtgataa ataaaaatcc caagaccatc catgcggagg aattggctgc caaagcccta
    86521 aaccagatgg aacattacaa gatcacctgt ttaatcataa tagataagaa aagaaaaccg
    86581 attgggattg tgcatatgca cgaccttttg gaagcaggcg tggtataatt taatacaaac
    86641 gcattaagtt ctgttacatg gtaggcaata tgcaattatt tttcaaatcc cccttgttcc
    86701 ccctttttca aagggggaag ggatttttcc cctctttaga aaagaggggt taaatgaaaa
    86761 cttaaaaatt tgttgcaaat ttttaagttt tcattagggg agatttgacc aagctcgaat
    86821 aaatgtgttt gtattagttt aacgatgcag atgtgaaaac aaggataata aaagggatta
    86881 aaaattggct gatttatcag tttatccttt taggaattgc gcttttcaac catcttcccc
    86941 gaaaaacagc cttgaaatgc ggttcctttt tgggaaaaat gtgttatttt cttatagtca
    87001 aggcaagaag acaaaccata gagaatttga aaatagcttt taaggaaaaa aatgagggtg
    87061 aggtaaaaag attaagctta gaggtctttg aaaatttagg caagaatgca gtggatgcca
    87121 tccggctaaa aaatgcaagc tggaatgaag taagccggat agtcgaagtt gaaggcatag
    87181 agcacttcga tagagcttac aagttgggga aagggcttgt tgccctgaca ggccatattg
    87241 gaaatttcga gcttatagca acctattttg ccttagccgg gtataaagtc tcagtaatcg
    87301 gaagagagct ttacgataaa agattagata ggttgttggt ggagaataga gaaaaagcag
    87361 gaatacagaa tattcccaca actgcccaag ccagagaggt gataaaagcc ttaaagtccg
    87421 gcagggcttt aggaatccta attgaccagg attccttcag ggtaagagga atatttgtcg
    87481 atttctttgg cagaccggct aagactccga ttggaccaat tcttttaact caaagaaccg
    87541 gctcccccat tgtgcctctt gccatagtaa ggaaagagga tgataattat aagatgataa
    87601 ttcgagagga gataaaaaca gaaactccag aggataaaga gaaaggactg atggagttaa
    87661 cccagaaggg aactaagttc ttggaagaga tcataagaga atatcctgac caatgggtgt
    87721 ggatgcacaa tcgctgggca agccagccaa aggatgaaac atttgattaa gaaaaggatt
    87781 ttcctctttc ttgtattttc tttaactttc tttttctgtt gcagtcagga ggaagaaaag
    87841 aagtctccca ctctggcaga tgttccggat caagtggtgg agaaaacaca aatggttttc
    87901 acggtggatg gagtaagatc aactgtcatt ggtgcggatt acgtagtcaa atatgaaaga
    87961 aaggatttga ccttagccaa gaaagtgcat gttgattttt ttgataagga aggaaaacat
    88021 acctcaatta tggatgcgga cagtggcatc ataaaggaga aaaggcagga attggaggct
    88081 ctggggaatg tggtggtcat ctcggataat ggggtaaaac tggagactga aagcttaaga
    88141 tgggaccctc gaaaacaaaa ggtgataacc gacgattttg tcaaagtcac caaagaggga
    88201 gatgtgattt acggatatgg gttggaagcg gatcagaagc tggagaactt tcagataaaa
    88261 aagagggtta aaggaacaat caaaaaggtg ccggaaggtg aactatagtc tagacaaaga
    88321 tgctttaagg gcagagaatt tagtcaaggt ttatcataag cgaaaagtag tcagtggcgt
    88381 ctcaattgag gtcaggccaa gagagatagt tgggcttttg gggccaaacg gagctggcaa
    88441 gaccactacc ttctacatga taattgggtt tgtacgcccg aattcgggga aggtcttgat
    88501 tggagaggaa gagttaacct ccttgccgat gtataagcgg gcacaaaagg gaatcggcta
    88561 tctttcgcag gagccatctg tttttagaaa gttgaccgtg gaagagaaca taatggctat
    88621 tctggagatg cagaagatat ccaaaaagga aagaaaggaa aaactcaaat cccttttagg
    88681 ggaattaggc atcgcgcatt tggctaagag caaggcctac actctatccg gtggggaaag
    88741 acggagagtg gagataaccc gtgctttggt cacccagccc aagttcatgc ttttggatga
    88801 gccgtttgcg ggaatagatc cgatcgcagt agaggatata cagaaaatca tttttgagtt
    88861 aaagcaaaag ggattaggga tattggttac cgatcataat gtgagagaga ctctctccgt
    88921 tacggataga gcatatataa tgtgtgatgg tgagatcctg aagtccggga cctctgaatt
    88981 tctggcttcc gaccctgaag ccagaaagat ttatctggga gaaagattca agctaaacta
    89041 agatttaacg ctttccgata cctttaaata gaggagaaac ctttaattat cttataaaga
    89101 tatgaaactt gaactccaaa tgagacttca gcagactctt gctcctcagc tgatacaatc
    89161 cctgaagatg ctgcagatgc caatcctgcg tctggagcag atcttaagac aggagttagc
    89221 catcaaccct ctattggagg aaatggattg tgatgaagag gaggaggcgc cttcaacaga
    89281 tgaggaggaa gttgacccca acctaaacaa gattgattgg gatgaatacc tgggagatga
    89341 ccacgaattc agggtgaagc ctcagagaga agagtcggag gatagattag aaagggtctc
    89401 ggtcttggag aagaccctat atgaccactt aatagagcaa ctcagtttca gtaaactgac
    89461 tccggaggaa tatgagatag gggaatacat catcggaaat attgacgata gtggttatct
    89521 cagttgctct ctggaggaga tagtcgattc ccttaagggc gatcctgctc tcaccgaaaa
    89581 aatgttgggg ataatccaaa cattcgaccc gctgggagtg ggagcacgca atctgaggga
    89641 atcgcttttg attcaattaa gggaaagggg actggaggga agtttggctt accgtattgt
    89701 agatgaatac ttaagcgact tagataaaaa gagccattcg caattagcca aaaacttagg
    89761 ggttagtttt gaagaggttc aggaggcgat ggatctgatt cgcaccctct cccccaagcc
    89821 aactatggga aggttcagtc cgggagctac cccggttatt ccggacctga ttgtagagcg
    89881 ggtaggagat ggatatgcga tttttcacaa cgaccggaat gtcccacgct tgaggattaa
    89941 tcccatttac aaagagttga taagaaagca gagcaccagt tcacccgagg agaaaaaata
    90001 tgttaaggag aagctggagc aagccaggtg gtttctgaat gccataaatc aaagacgctc
    90061 cactatgatt agagtgatgg agacggtggt ggaggagcag aaggacttct ttgaacatgg
    90121 tatgtctcac cttaggcccc tgaccatgca agccgttgcg gagaagttgg ggttgaatgt
    90181 ggctaccata tcgcgcgtgg ctaatgataa gtatgtgcaa acccctcagg gcgttttcca
    90241 gctaagatat ttctttaata ccggcgtgca aaaacaggat ggcgaggaac tgtcccggct
    90301 taatgttaaa cagatgctgg aagagataat taaaaaggaa gacccccttc aacctttatc
    90361 cgatcaggag atttatactc gtctaaagga aaaagggctc aatatcgctc gtcgcaccgt
    90421 gaccaaatac cgagaagaac tgaagatact cccggcgaga tttcgtaaac gaatggtaaa
    90481 ggagaaacta gaaaacccct cgagtcttga gctagaagag caatgatgct caaatttacg
    90541 ttatttaggc cttatcccct ccaaccactg aaagtcagtt tgccataaag acgacatcac
    90601 aggaggtgat gtatggaaag aaatttggct ttggagttcg tgcgagttac tgaagcggct
    90661 gccttggaat gtgctcgctg gatggggaaa ggcgattcta attcagcaga tgaagcggca
    90721 gtcagagcta tgcgcaagat gttcaactcg gtggatattg atgggacggt agtcattggt
    90781 gagggggaaa gggataaggc tcccatgctc tatattgggg agaaggtggg aacgggaaaa
    90841 cctccgaaag tggatatcgc cttagaccct ctggaatgca ccaacagcgt tgcctgtgga
    90901 aggtggaatg ccctgtcggt cattgccgtt actgaaagtg gtaattttct tcatgcgccg
    90961 gatacttata tggagaaaat agcagttggg cccgaagcta aagaagcgat tgaccttaac
    91021 ttaagcccgg aggagaactt aaaaagggtt gccgaggttc tggactatca cattgaggat
    91081 atcaccgtgg cgattttgga tagaccccgg cacgaggatc tggttaaaag ggtgcggaaa
    91141 gccggcgcga gaattcagct gatttcagat ggggatgttt ccgcggccat cgccactgcc
    91201 ttaccggata ccggggtgga tgttctgatg ggaatcggtg gtgctccgga gggagtgata
    91261 gcagcagcag cactgagatg tgtgggcgga ggaatgcagg ctcgactggt cccacggaat
    91321 gaagaggagg aacgaagagc caagaagatg ggcgtaaaag acataaacaa gatttttcgg
    91381 gaagatgagt tagctggggg gaagaacatt atgttcgctg caactggagt tactgacggg
    91441 gatatgctca aaggggttag attctttgga gggggagctt atactcactc cattgttatg
    91501 cgctccaagt caggaaccat tcgtttcatc gagaccactc actttttcga ccgtaaaccc
    91561 aaatactagc cgaaaaggag ataaagatgg agaccagaat taccgctcga cacttcgatt
    91621 tgacccctga actgaaagag ttctgcgaga aacagatcag tcgactggag agatatttcg
    91681 accatatcat cgactgtcac cttatcttgg atttggagaa atcgagaatg acggccgagc
    91741 ttaaacttaa agtccatggc acggccttaa atagcaagga gagctccttt gatatgcaca
    91801 tttcagtgga gaaggtcctg agcaagatag aggttcagtt gaaaaaatac aaagccaagt
    91861 tgaaggataa gaagccgaga aagaccaaag cgattaagca ggagctgaac gccaaggccc
    91921 tggcctgtga gctcgaagaa gaaagaccat ctttctgagg gaggacttag cgtggaaacg
    91981 ataaccgtag agaagatgta ccaggagagg aaagagcatc tggagttggc tttgctcacc
    92041 aattcggatg gtctttcaaa gaggatccgc aacaacgaga ttcatcgtcc gggactggct
    92101 ctggctggct tcattgacag gtttgcacat aatcgcacgc aggttttggg tgaaaccgaa
    92161 atggcctata tccaaagcca ggatgagaaa aataaaaggc agaccctgga gagtttcttc
    92221 aggttcgata tgccttgtgt agtaatcacc aagggaatta atcctccagt agaattgatt
    92281 cagatagcca atcagaatgt gacttcggtc tttcaaaccc ggctctccac cacggaattc
    92341 atcacccgtt tgagcgtcta tctggataac ctattcgccc cgcacataac cattcacggg
    92401 actctggtgg atatctatgg agtgggtttg ctttacactg gaaaaagcgg catagggaaa
    92461 agcgagtgcg ccttggatct ggtggaaagg ggacatcgtt tagtggcgga cgatgtggtt
    92521 aaggtcatca agaaagctcc gcatgtcatc gttggcacag gaagtgagct tctgggacat
    92581 catatggagg tgagaggaat cgggatcata gacttagaga agctttttgg aatcagagcc
    92641 atacgcgttc agaaaagaat cgaggtggag gtgagattag tcctatggga agatgaagag
    92701 gaatacgaac gcttaggaat tgaggataaa tttaccactg tcttgggagt ggagatgcca
    92761 gtggtgacca ttccagtatc tccagggaaa aatatcaccg ttatctccga agttatcgct
    92821 atgaaccata tgctcaaagt ctatggtcag gacatagcca aagcattttc ccgcaggcta
    92881 agcgacgaga tataccgtaa gaagatgtcc aacgaatact tagagagcga cacggagtaa
    92941 aaaggatgtt cgactgcata atcctgacac atggaaactt ggcgacctcc ttgaaggata
    93001 cggtggaaca gatgatgggc aagcaagtgg ggatgatgat catatccaat caaggaaaag
    93061 accctgaagg attgctctcc aagttagagg agcttgtttc aaaaagcaga tccaaagaac
    93121 tcttcctctt tgttgatctt tatggtggca gttgttggca ggtggcaaag aaattcagct
    93181 cgaaaagagg ggatgtggtt ctgataaccg gggtgaacct gccgatgtta gtgcaattct
    93241 taagcaagag agagaagttg gacaaatttg aattgttgga gaaagtggtg gaaagcggta
    93301 agggtggaat cgtaaccgag ttttaacgcc cgatgatctt ctttagatta gatgaacgtt
    93361 tgatacatgg gcaggtggtg accgggtggg ctaacgcctt aaagctcgac aggataattt
    93421 tagctaatga ccaggcggca agaaatccct gggaaagaga gctatatctg gcttgccttc
    93481 ctccagaaat agagggttcg attctgaccg tggaggaagc cactaaatac ctatccaatg
    93541 gggaattcaa gaatagaaaa atgatattga tagtcaactc ccctgccgat attcttaaaa
    93601 tgaaagaggg gggaatccag attactgagt taaacatagg agggatgtat caaaagcagg
    93661 ggcggataca gattctccct tatctccatc tatctttaga ggagatagct gatttaaaga
    93721 agttgttcag tcagaatgtt aagtgcattt gtttggatgt gcctctagga gagaaaagaa
    93781 atctaaaaga gcttttcgga aaattaaacc tttaagaggg ttgttactaa tggcatccga
    93841 tagaagtgta gaattaagag tgggaatagt tgtgatggcg gcaatcatac ttttgatctt
    93901 gggaataatc tgggtaaaac aatatcgttt caacgtggtt aggtatgaat atagtgtcat
    93961 ctttcccaat gtgggtgctt tggaaaaggg agacccggta accatatccg gagtgaagaa
    94021 aggggaagtt gaacagataa atctttacga gggagacgtc ttggttgcct ttaacctgac
    94081 tcaagatgtg gtcttaaagg aagatgccaa atttagcgtg atgaacatcg ggcttatggg
    94141 tgagcgttac attgaaatct tcaccggtca tagcgataag ctattggatc tgtccaagcc
    94201 ggtgagggga tactatgaca ctggaattcc tgaaataatg gggatgatgg gtgaggcagt
    94261 aactgacatc aggaatctgg ttagtcatct ggaaggggca attgggacca aatggtcgca
    94321 ggaatccata attgaaatca ccagaaattt gcgtaaggtt tccaatgacc tgaatcagat
    94381 gatggaaaag aacaaagata aatttaatcg ctcagtagat gacctttcct atgcctcttc
    94441 tgaattgaaa aacctggtca aggataataa gggtaagctc caatccgcag tggacaactt
    94501 tgccaacgcc tcaaacagat tggatagcgt aaccaccagc ttgcaggagg tgtccacttc
    94561 cctcaagagt ttcactcaaa agatagaaaa aggcgaaggg actatgggga agttggttca
    94621 ggatagctcc ctttatctgg atttgagaaa gaccactcag gatttggata gcttgattct
    94681 ggatatcaaa gccaatccga aaaagtacat aaagctcaaa gtgttttaat gaatgaagaa
    94741 gataaaattc tcctttgcca tacacgatca ccagcccatc ggcaacttcg agttcatctt
    94801 tcgaaatgcc cacaataggt gttatcttcc ttttttagag cttttggaaa aatacccttt
    94861 ctttcgaata tcccttcatt tttcaggcat cttacttgaa tggatcaaga ataaagagcc
    94921 aaaaactttg gatttgataa gaaggttagt ccagagggga caggtggaga tggtgggagg
    94981 agctttttat gagccgatac tttcagttat cccggacgag gacaaacttg gccagataag
    95041 aaaactaagc aagttcatcc gggatgaatt caaatgcgat gtttgcggta tgtggttggc
    95101 ggagagggtt tgggagccgc atctgccaaa gcccatagcc gaggccggga taaaatacac
    95161 agttctggat gatacccatt tcaggtattc cggtttaaaa gatgaacacc ttttaggata
    95221 ttacataacc gaagagcagg gacataaaat atctctcttt ccgggaagta aaactcttcg
    95281 ctatgccgtt ccctttaaag agccagagga gataataaat tatctcaaaa gtatagcgga
    95341 tgaggatgga aaaaatttga tagtctatgc agatgacgga gagaagtttg ggctttggcc
    95401 tgggacttat aagcattgtt acgaagacag atggatagag aagttcttta aggagataga
    95461 aaagaattta gactggattg agatgattca tttaaaagaa gctttagatg agattccacc
    95521 tttgggtaga atttatcttc cggttgcctc ctatgaggag atgatgcaat gggctctgcc
    95581 agcagaggtt tttgaggaat atgaggattt ggaaaagatc ctaagggaga agcaactttt
    95641 tgaaaagtat caggttttcc tgcgaggcgg gtactggagg aacttcttat ccaaatatcc
    95701 cgaatccaac cagattcaca agaagatgct atgggtaaga gaaaaactta acacaattaa
    95761 gccttcctct caggctcaaa ggaaaaaact ggaccaagcc caggacttct tatggaaagg
    95821 ggagtgcaac tgcccatatt ggcatggtgt tttcggcgga ttatatcttc cccatttgcg
    95881 ctcagccatt tatcagaact tgatacaagc tgaaagagta ctggatgatt taattcataa
    95941 taaaggggat tggatagatt ataaagtctt tgactttgac aaggatggct cggatgaaat
    96001 cataattgag tcccccaaac tcaatctcta ttttcatccc cattggggag gggcgcttta
    96061 cgaattagat tacaaaccga aaccttttaa tctattggac atcttgagcc ggcgcaggga
    96121 agggtatcac aggaaacttt tagagcttaa aaagaatatc aataaagaca accaggtgga
    96181 aagcattcat gatatctttg tggcaaagga ggaaggttta gagaagtttt tgaattacga
    96241 ttggtaccgg cacggctctt taattgacca ttttttaggt gaaaatgcaa aactggagga
    96301 tttctccaaa tccaaatatg gagagcaggg agattttgta aatcagcctt ttgtgcctaa
    96361 aatcgaaaaa cagaaaagta aaataatctt gagtctaaaa agagatggat ttgtctgggt
    96421 gggaaaaaac aggataccga taagtctgga aaagaaaata aaaattagtt caaaaacatc
    96481 tgaactgaac atagtttata gtttgaccaa taactccaac caggaaatcc agctttggtt
    96541 tggagttgag tttaattttg gaatgctttc cggggaagat actaaaagat attattatat
    96601 attgggtcag aatccggaaa acagcaagtt gaatagcgta ggtcaatctg cggatactgg
    96661 ggaattcgga ataaaggatg aacatctggg tttgaacata aatttaaaag tcgataaatc
    96721 tgcagaattg tggcgttttc ctattcagac tatatccttg tcagagagcg gctttgagaa
    96781 gaattaccag tcttcggttt tattccccaa ttggaagata aacttagccc cgaaggaaac
    96841 ttgggaagtt agcatcattc aagaaatctc caacttataa tccaactctt cttaacttcg
    96901 ctcttatctt atctatatgc gttcccttcc agaaaacctt atcacatttg ttacagatga
    96961 cgaaatcttt ttgggttttg aaaacgtaag gaggaatttt atcttttgct ttgtcttttt
    97021 cgacttcttc taagatattg ttacacaaca agcaacgaga gaaaaaacct tcttttttcg
    97081 gttttaattc gaaatactca attacttgct ttagctgttc taagggatta tcgcttgtta
    97141 tcaaaagttg attttgggct atcttaaatc ttgccaattt agtgtcgcgg gttaagataa
    97201 ccctgttttg ttccaaggat tgcctgatta gtttatagtc atccagatct ttttcaaata
    97261 gcgtatcata ccctaaggtt ctcaagagct tggctaactt tcccagatta tcatcacaga
    97321 tgaatttcat ttcccctccc tttttatgat attgtctgat tttcgtttgt caacacctaa
    97381 gtttttcagg actatcccca cttttttcca agacattcca gaacgtgtta atagctatcc
    97441 ctcagtttct ctctcccctc aggggagaga gaaagagtga gggggaagaa aaagatgcca
    97501 caatagcttc acagccgtga tgctcctcac cctcaccttg atcctctccc tcgaagggag
    97561 aggataaaaa aggagtaatc cttcttttaa agacaagaat tgccttcctg ttctttccga
    97621 cctttaaatt ctccttgcct cacgaacacc ccccatctct ccataatact gtttttggcg
    97681 acaatcctgt taagttttgg ggtatttgct tgcgtcagag caagccctat cttatattgt
    97741 aggatatgaa aaaagataaa gctgaaaggc ttaaattcag acaaacagca gttaaggcgg
    97801 ccctagaagc cggagagatc ctgcttgaga accgaggtaa aagaaataaa atcgggtaca
    97861 agggagaggt gaatttagtt acagaggtgg atttcctgtc tgagaaagcc atactcagga
    97921 taataagaaa aaactttcct gaccattcca tattaactga ggaaaccatt ggtcaaagaa
    97981 gagattcgcc ttattgctgg atagttgacc ccttggacgg aacaaccaat tacgcacatg
    98041 gttttccagt atattgcatt tcaattgctt tacagaaaga caaggaggta atcttggggg
    98101 cggtttataa ccctcttttg aaagagttgt tcttagctga aaaagggaaa ggtgctttct
    98161 tgaacggcaa aaggatattc gtgtccaaaa ccaaaaaact ctcccaatct cttttggcaa
    98221 ccggatttcc ttacgacata aggaccagca atgaaaacaa tctggattat ttcaacaaat
    98281 ttgcggtcaa agctcaagct ataaggcggg ctggttccgc ggctttggat ttgtgttatc
    98341 tggctatggg aagattcgat ggtttttggg aattaaagct ctctccctgg gatacagccg
    98401 cagggatttt gatcataact gaagcaggtg gaaagacaag cgatttttca ggcaagaagt
    98461 ttggtatctt ctccaaaaag ttagttgctt caaacaattg gattcataga gaaatacttg
    98521 aggtcataaa ttcgtaattc attgtgaagc ttggacatac aatagtagaa ttaacttgac
    98581 aaaatcttta ttttttgtat agagttatta gttcaaacgc attaatttac gttcaataaa
    98641 tctcccctcg cccctctttt tcaaagaggg ggaaaaagag gtctccccct ttgaaaaagg
    98701 gggatttgag ggggatttta aaatagattg catattgctc taatgtgtaa caacatttga
    98761 tgcgtttgct atagtttaag cgcatcaaat gaagtatcat tgaggccgtg tagtaggacc
    98821 taagagtgcc cattttgtca ttctgagcga agcgaagaat ctaatagttt caataggtta
    98881 gaaaatcgag attcttcccc atatgggtca gaatgacatt gcgacacggt ctgattgcga
    98941 ggagtcccga caaaatcagg acgacgaagt cccgtcttta cgggacggca ctcccccgat
    99001 ctcaagatgc agaattcgca taaatcgggg tcgctcgccc taaaccagaa cggtttgggg
    99061 ctcgcaatga caatgcaaag tcatttagtg agtgtgaata taatatgaag agagaagata
    99121 gtataagagc cgatatagtt gcggtttgcc aaaggatata ccaaaatggg tttgttgcgg
    99181 caaatgatgg aaatgtcagc gtaaagttgt ctgatagtag aatattggtt acccctacag
    99241 gaagaagtaa aggctctctg acccgagaag agttattaat aattgattcc aagggaaaaa
    99301 tgctgaaggg gaacttgaag ccgacttcag agttgcccct tattctttat gcctatcaga
    99361 aaagaaagga tattaaggca actgtgcatg cccatccgcc ttatagcaca agcttgggtc
    99421 ttgcagggat tggattgccc aaagatgtct tgccagaggt attcttgact ttaggggaag
    99481 tccctctggc caaatacggc accccttcga ctgcagagtt ggctaattca gtttctgatt
    99541 acataaaaga ccatgatgca attttgttga aaaatcacgg agttttgacc ttaggcaaga
    99601 gcgtggaaga ggcttattat aaacttgaaa gagtggagca tttcgctcat attttgtggt
    99661 tggctattgg tttggggaga gtggatagac tttcgaagaa ggatatgaat aaattgataa
    99721 aactaaaaaa acctagataa aaggggtgct tatgaaaacg ctattgaagt gcttatttgt
    99781 agttcttatt gcttcagtag ttggtttctt gttcggtggt tgttcgaaga agacgacttc
    99841 ccctactact cccggagaaa tcacagtaac aggaaaagtt gacttacctt caggagcaag
    99901 tattgagccg gaaagtttgg ttgttctgtc cccggtcaat tcagacgcta ttggttctga
    99961 tagttcgttc agtgctaatc ttcctccgaa taccgcgata ggtgtaatgt gtattactga
   100021 caagaatgat gacccgcttc tcttaggata tatattcacg agcggtttta atccaacttt
   100081 ccccaaagtt aaatctgcat acaaaaccaa tattatatca aattcgggtg aagcagtaac
   100141 aatcaatagc cagaccacag ctctttcttt gattatgtta agtcctcttt taattggctc
   100201 cagcccagag cagagaaatg aatttgccca acgagcggtg gaccatcagg attttccctc
   100261 ccttgtatca gacattgaca atatgatagt taataacccg gaaaactacc taacagacag
   100321 caccaaaagt ccacagatat ttcaaaaagc ggctagcata agtttggacg ttctaagaag
   100381 ctttgaacaa gagagtttaa tgaaggcgca gaaaactctc agagccaatc catccataga
   100441 ggatgccgca gggacaaagg taaatttcat aaatccttat tatatcttct atggaacagg
   100501 tatatacgat tattctacga gtgacgagat tgatgttttt gtgcagagtc cgaggaagtt
   100561 attttgggga acaattcctt atttaggtcc tgatggcaag actgaatatg acctcggcaa
   100621 cggtgatttt aacatccaga tatataaggg ttttcattgg tttccactaa acctaagtga
   100681 tcccaagtgc atagctactt ggtataacac agggcaaggg ctattaaata tattagattt
   100741 aatcgtctgc ataccaggag tgacgaactc aatttccaca ataggtataa ttgtccaaaa
   100801 tgccagtgat tttaaaggcg cagtagagtt aggtgcagct ttaagggagg gagacacctt
   100861 cgaagccata ttaggttttt taaggtttat aagttccaac acataccaga tcttagttag
   100921 tgtattgcaa agctcttcga cacctgaaaa tatctccaaa tatcttaatg ttacaaagaa
   100981 cgtggcaggt aatgttacca ttgctttaag ggccttgaat gccgcaacta aaatcccttt
   101041 cttcttagat attgcctatc ttgcaccacc cgagcttgag tatgatgtat ctcaggttga
   101101 tggaaacttg gggttcatta acagaccgtt gggaaagata gctttctact caactagaga
   101161 cggaggatac gataaaatct atgttatgaa tgcagatgga acccaacaaa cgagattgac
   101221 agacaatact tctagtaatc aacacccttc ttggtctcct gatggagaca agatagcctt
   101281 tgtgtcacat agagatggat acagtgaaat ctatgttatg aatgcagatg gaacccaaca
   101341 aacgagattg acaaatagtc ctagtgggga ttattggggg aatttccatc cttcttggtc
   101401 tcctgatggt caaaagatag cttttatgtc agatagagat ggaaattgga aaatctatgt
   101461 tatgaatgca gatggaagtg gtcagatgaa tttgacaaat aatactgttt gggagatgta
   101521 tccctgctgg tccccaaacg gagaaaaaat agcctttaca agagccggac agatgttggg
   101581 gatttatgtt atgaatgcag atggaaccca acaaacgaga ttgacagaca acccttactt
   101641 ttttaatgat caatatcctt cttggtctcc tgatggtcaa aagatagctt ttacttcaac
   101701 tagagatgga tacagtgaaa tctatattat gaatgcagat ggaagcgagc agacaaactt
   101761 gacaaataat gatagtggtg atgggagtcc ctgttggggt cctgatggag aaaagatagc
   101821 ttttgagtca tacagagatg gaaattggga aatctatgtt atgaatgcag atggaagcga
   101881 acagacaaga ttgacattta atagtattac gggacactat gatgggtgcc cttcctggac
   101941 tcgttgagag tttgaattac tgatcttcgt ataagtaatg aaaaaatttt tcatcgacca
   102001 caatctcctc ttttttataa tcccccttaa tccccctttt ataaaggggg acaaaggggg
   102061 attaaaagag agaagaaaga cttttcattg cttaggcgta actcaataat acgtatgcga
   102121 tacgattttt tgaagtatca aaaacagtcc ttggcatagc agaagtggaa aaaaataata
   102181 aaaacgggct tgacccttcg acaggctcag gatggtgagc aaagtcgaac cataaattaa
   102241 gcccctacgg aaagacaatt ctaagttttg actttcgggg ttggagaatt tgtaggggtc
   102301 ggattccctg gccatggagg tcgagcggga agagacgggc aggcatccga cccgaaagat
   102361 acaaaatagc gggttcgatg tcgtcaagat gctgtgcaaa tccctggccg ggcaggcgca
   102421 cccgtgcaaa atctttagaa cagcaagatt gcttcgcttc cccgataaaa cggggtcgcc
   102481 cgcaatagca agataatttc ttgtttgtca ttcctgcgga agcaggaatc cagaagagaa
   102541 gaaacaggta tggatccccg attaaaccct cggggatgac agaacaagac aagcggagat
   102601 tgaactacaa aaggaccacg cctgtaaagg cgtgagtacc tatggaatga aagtaagttg
   102661 ataaaacagg cataatctta tgataaagcg aatcatcata gataaatccg acagattgca
   102721 gagaattgcg ccatttcccc tcctggaatt ggaaagagcc aaaagaagat taaagaggat
   102781 aaatggtgag ttgatagatt taggagagtt ctctggtggt ttgaaatcaa tgcccggctt
   102841 gtcgcttgat aaaatagaac atctggatag tagttttgtt tccaacctaa aaaagaagat
   102901 tgctctttgg tttgagaata aatatggggt caaattgaat ccggaaaggg aggtcttccc
   102961 gtttgtggga agaaagagaa tcattacaaa attgactttg gtttttttga atgaaggaga
   103021 agttggtttg gttcctaacc caggcaatcc gatttatcgg actgcaatca tcttggcaga
   103081 tgggaagcca gaggagtttc ctctcttgga aaggaatgat tatctgccca atctgagtta
   103141 tgtgttgaac aaaaacaaga gggcaaagct tattttctta aactatccca atgacccaac
   103201 ttcaagtctg gctgatttga atttttttgg ggaagcggtg gaaatagcca gccagaataa
   103261 tatcttactg gtaaacgacg ccacatatag ccagatatat tatgatgagc actcgccctg
   103321 cagtttgatg caaattaagg gagcgaagaa agtcggagtg gagatttatc tttttagagc
   103381 tcctttgggc gaagttgggt tcttagtcgg aaatagagag atcatatccg gactggtcca
   103441 gttggataag gcgctggatg ataaagatta ccttatgttg cgacaattgg attccatatt
   103501 ggatgactat cctgggaaca taaactcata taatcaggaa cttgcccaaa gaaaagatat
   103561 tctatatgat gagtttttgg gcttgggatg gaaggccaga aaacccagaa gcacaccttt
   103621 tttctggatt aaggttcctc caccacatac ctccatgggc ttttctcggc tacttttaag
   103681 aaaggcagga gtggtggtgt ttcccgggtc aagttttggc gaggaaggtg aagggtacat
   103741 cagaatcgca ctgaatgtcc cggttgatgg gatcaaacag gcctgtcgga ggataaaaga
   103801 acattctcat ctctggcaga agaggtactc accttaaaag ggattataat gggaattatt
   103861 agcttgcatt ttgtgcctgc caagtccccc ctggccgggc aggctgactt ggcagaagaa
   103921 tgctgtcagc aagaactttg ggattgcttc gccccctacg agggctcgca atgacaacac
   103981 tacactgtca ttgcgaggag tccagccaaa ggtgagacga cgaagcgatc tcgttttaat
   104041 cgtaggggat tcaagaattt gaacctttat aggaaagtgt gaaatgggaa tcatccgttt
   104101 gcacaatatg accttctatg gttttcatgg agtttctccg gctgagaagg aaaccggcag
   104161 aaggtttgag gcggatgtcg aattgcattt agatttggat aaggcagcac agagcgacaa
   104221 acttaaagat acagtcaact acactgaggt ttataataca gtccagcagt tgattatgga
   104281 gaacaagttt tcgcttttag aaaccatagg agtgaggtta gcggaaaaga tattagagaa
   104341 gtttggcgtg gaagaggtgc ttgttcgtgt cagaaaaaag attccaccaa ttcccggaaa
   104401 tttagatgat attgaagtgg aagtcagaag ggaaaggaaa aaggaataag gaaaaaagga
   104461 aaaagggaaa aagaaagaag aacatttgga gattgtatat attggagtcg gctcgaactt
   104521 gggggataag aaaagtaaca tccagaaagc aattgatagc ataaaagaaa aaaggatatt
   104581 aagggaaatt aaagcctctt ccctatattt gaccgagccg gttggaaaaa aagggcaacc
   104641 tgattttttg aacttagtta tcaagggtaa aaccgaactt accccctttg aattgctcga
   104701 ctctcttttg gaaatagaaa agaatttagg tagaaaaaga aaagaaaagt ggggacccag
   104761 ggagatcgat ttagacatcc tgttttatga tgatatggta gtgaaccaag aaaacctggt
   104821 cataccccat ccggaaatac cgaatcggag atttgtcttg gtttctttaa tggaactctc
   104881 accagatttg aagcatccgc ttttaaacaa gaacataaaa cagcttttaa aagacacaaa
   104941 agaccattcc aaagtagaac cttacggggg aatttatgca ggagaatagt tatattgcag
   105001 tggaaggggc tatcggcgta gggaagacca gtctggctaa gctcttgact gagaagacaa
   105061 gtgcccgtct ggtcttagag caggtggagg ataatccgtt cctggatgat ttttacaaag
   105121 atagagcaag gtatgctttc cccgcccaga tattttttat tttaactcgt ttcaggcaac
   105181 agcaggagat gcacaccacg gacctttttt acaagagggt gatttgcgac tacatctttg
   105241 ccaaggatag aatattcgcc tcaatcaatc ttgcctccag agagcttcat ctgtatgagt
   105301 caatcctttc ccttttggaa aaggacatag ccaaaccgga tttggtgatc tatcttcaag
   105361 cctcaactga aactttgatg aagagaatca gaaggagaga tagggaatat gaaagaggca
   105421 tggattatca gtatttacag gagttaaatg aagcttataa taatttcttt tttcactaca
   105481 cccagacccc gcttctggtg gtgaacacgg atagaataga ttttgtcaag aatcccaaac
   105541 atttagatga tttagtggag aagatcaagc aacctcattc aggcacaaaa tattatgtcc
   105601 cttttgggga gtagagaaaa tctatgacta agcaggataa agttacagtt aaaagcttca
   105661 tccagatgaa aaaagagggg gaaaagattg cggttttgac tgcttacgat ttcttcaccg
   105721 ccaagatcct ggatgaaatt ggaatggact caatcttggt tggcgacagc gccaatatgg
   105781 ttttttatgg cgctaaaaca actctttcca taacaatgga acagatgatc tatcatacca
   105841 aagcggtctc ctctgccact aagagggctt tggtcattgg cgatatgcca tttttatctt
   105901 atcaaacctc ggtttcggag gctgttcata atgccggact tttcctaaaa gaaggtgggg
   105961 cagaggcagt aaagattgaa ggtggattgg aaatgattga taccataaag aaaataattg
   106021 atgcaggaat accggtgatg gggcacatcg gtttagttcc tcaatccatt caccgttttg
   106081 ggggatacca tacccaggga aaagaccaga aaactagaaa atatcttttg gaaagtgcca
   106141 aaagcttaga acaaacaggg tgcttctcaa tggttttgga agccatccca attgagttag
   106201 ccaaagagat aacccaatcc attagcattc ccactatcgg cataggggct ggagtctcct
   106261 gtgatggtca ggttttggtg ataaatgacc ttttgggtct atttgatgaa tttaagccga
   106321 aattcgtcag gcgctatgcc cacctttcag acgagataag aaaagcttgc ggggaatatt
   106381 tgaaagatgt gaaagcaggg ggttttccgt ctacagaaga aagctactaa aaaaattaaa
   106441 aattaaaaaa taaaaatgaa aaattgaaaa gcaaaattca aaatacggaa caaagagaaa
   106501 aatgaaaaat tgcaattcaa aattcaaaga tgatataaag aaaagaacct atttttatgc
   106561 attggaggtt atgcggtcgt tagagcagtt gccgaaaaac gatttttctg ctgatattgt
   106621 tgctaaacag ttgctacgca gcgctacaag cataggtgca aatataattg aggcacaagc
   106681 ggctggttct aaaagagact ttaccaatta ccttcgctat tctcttaaat cggccaatga
   106741 aagcaagttc tggattgccc ttctgagaga ttccggtaag ttaaataggg aactctccaa
   106801 ttctttgctt gaggaagcta aagaattggc taatattttg ggcaaaagca tccttacttt
   106861 aaaaaacaaa aacctataat ttttattttt aaattgtaat tttaaatttg tcatttttca
   106921 ttttggattt attttgatga agataataac ctcgccaaat agaatgcagg aaatctgcaa
   106981 caagatgcaa agggatggca aagtcgtcgg ctttgtccct acgatggggt atctgcacaa
   107041 aggacatcta agtctagttc gtgttgctaa gaagaaaagc gacgttctag ttgtcagcat
   107101 ctttgtcaat cctgctcaat ttggaccgaa agaggatttc aaaagatatc ccagggattt
   107161 cgctcgggat ggagatttac ttaaagaaga gggatgcgac tacgtttttg ccccaaagac
   107221 aaaaaatatg tatcccgaaa gttattctac cttcattgaa gtcaatggat taagttcgaa
   107281 attagaaggg gcttctcggc ctggtcattt taaaggagtt accacgattg tagcaaaact
   107341 ttttcatata gtacagccag acattgcagt ttttggtcag aaagatgccc agcaagtggt
   107401 aattataaac aagatggtga aagacctgaa tttcaaaacc aagatagtca ttgccccaac
   107461 tgttcgagaa aaagatggac tggcacaaag ctcgagaaat aaatatctct ccccaaaaca
   107521 aagaaaagac gccacggttt tgtatgagtc tctggctttg gccaaaggtt taataagaaa
   107581 tggcgaaaaa gacgcaaaga ctatcgtaaa taaaatgagg gagctaataa acaaagaaaa
   107641 gggtgccaaa atagactata tcgctatagc caatacggaa aacctgaatc caatagaaaa
   107701 aatcaaaggt gaagttctga tatccttagc agtgcagttc ggcaagacca gactgattga
   107761 taatatcaaa atgaaaattt gaggagaaaa agatgttaat cactatctgc aaatcaaaaa
   107821 tccatcgagt caaagtcact gaagctgatt tagactatga gggtagtata actattgatg
   107881 ttgagatttt gaaaaaggcg gatattcttc cttacgagaa ggttcaggtg gttaacaaca
   107941 acaacggagc cagatttgag acctatgtta tccccggtaa gaagggttcg ggtgtcatct
   108001 gcctaaatgg tgcggctgcc cgcctgggcg aagttggaga tatcctgata atcatctcct
   108061 attgtgagct ggaaaaagag gaagctgcca aatacagacc aaaaattgtg ctggtggata
   108121 agaagaataa acccattaaa taagatgaaa aagttagctg taattatctt agccgcaggc
   108181 aaggggaaga gaatgaagtc ggatctgccc aaggttttgc acaaaataga tagcaagccc
   108241 ttgatagatt atgtcatttc aactgcaaga gaaataaatc cgcaaaggat aattgtggtt
   108301 attgggcata aatatcagat ggtgaaggag tatttaagag atcccgatct agaattcgtc
   108361 atacaaaagg agcagttagg cacaggccat gcggttatgc aaaccgaaaa aatctttggc
   108421 gattttgatg gtacaattgt ggtattatgt ggggatgtcc cttttttgaa ggcagagaca
   108481 attaaggagt taattcagga gcatcataaa gccagagcct ctgccactgt cctgaccaca
   108541 attttagatg accccaaagg atatggcaga ataaaaagag atgggaacaa tctggtggag
   108601 aaaattgtag aagaaataga taccacaagc caggagaaaa agctcaagga gataaacagc
   108661 gggatttttt gttttgaatc taagttgctg tttgagacat tgcatcaggt cgataccaac
   108721 aataaacaac gggaatatta tctaaccgat gttttaaagc taatgcggga taaaaagctg
   108781 ccagtagcag ctgtggtttg tccttgtgct tctgaggtta gtggagtcaa ctccatagag
   108841 caacttaaac aaatggaaaa agcatctaac tttcgtgtca agtaaacgta tcataaaaag
   108901 agttgacaaa taaggtattg ggaatgtata tttcaggtgt gcttatgaag aaagcgatta
   108961 tactattcgt cgttttagga ttcttggcca caggcacggc ttccctctgg gctcaaagcg
   109021 tagaacaatc gaaagtatcc ctgactggcc agacaaagtt ctccagctac agtctattgg
   109081 atccgagcaa aatagattta catcatagtt attcttttgc ctatttctca ggtagccagg
   109141 gaagtttcgg cttggggatt tatactgcag ttttggatta tcgcatctct tctcctttga
   109201 gtttgagatt gggcctttct tatctacatc aacccttagg acgattgagc aataagacta
   109261 accttaacat aaagcagtcg attttaccga gctttgaatt gcgatatgaa cccaatgaga
   109321 aaacttttat ctttttaggt ttctccactt caaatcctta tttttttcaa acagaatagc
   109381 ctcaagaatg gatatgttca tcttaaaaca ggtgttcaaa gaggagtgat tacaccctag
   109441 cccttttata tttcctgaaa aatcttcatt atgcccaaga gaagcgaatc agcacatttc
   109501 tacaagagaa gagaaaagaa gaaacaaagt cgcgaggtta aggactggat aataatcctc
   109561 ttgactctta ttctagtggt ctatgctctt tccctggcta gtcatttgac tttgaataaa
   109621 gcccagggct cgggaaccga agaaccaacc taccttagag tgcagatact gaatggttgt
   109681 aaagaaagcg gactggcaga aaaagtccgc gaccagttga tttccaaaag atgggagaag
   109741 ttcgctttcg atgttgtgga tgtgggtaat tttggagatt ccattatttc ccaaaccttg
   109801 atactggatc gtaaagggca ggataaaaga gtctttgagg tggcaaatat tttaggaata
   109861 aaaaaagaga atgtgctttt gaagagattg gaagacaact acctggatat agacctgtcc
   109921 ataatcttgg gggctgatta ttcccggata ttcaagaact gaattcaaaa aaggagatgc
   109981 aaaagtagat tccagaaagc tggctaagct ggcaggtggg ttagccttag agaaaaaggg
   110041 tctggatata agggttctgg atttaagaaa actcacctcg gtaagcgatt tctttgttat
   110101 ctgcactgcc agcgtggagg tgcacgctaa ggctattgct gattggatag tagagaatct
   110161 ggaaagaaag ggattcaagg tctggcacaa tgagggttat caggcttgcc gctggatact
   110221 tctggactat gtggatgtgg tggtgcatat cttcctgaag gaagtgcggg agttttacgg
   110281 cttggaaaag ctatgggggg atgctccggc catagaaatt tacgataaac catcttccaa
   110341 aaaaacaaat aagaagactt aagttgggca tctagcagtc gatatactaa gatagaacct
   110401 caaacaaaac tttcttttca attctatatt gttctgttgg aacttcaaat gaaaaactta
   110461 aggtataatc cgataagttg gataaatcat atttcataaa agaggtggag atttatggat
   110521 atcgtggaac tcaaaagcaa aaccattgca gagctgacag gtctggctga agagctggag
   110581 ataccgggtg taagcggatt ccggaagcag gagttaatct ttaagatctt ggaagccaag
   110641 acagagaaaa atgggcttat tttcgcggaa ggggtgttgg agatattacc tgaaggatac
   110701 ggattcttgc gctcaccgga ttataactat cttcccggtc cagacgatat ctatgtctcg
   110761 ccttctcaga taaagaggtt cgacctgcgc aagggggata cagtctccgg gcaggttagg
   110821 ccgcctaagg attcggaaaa atattttgcc ctgcttaaga ttgaggcggt gaattttgaa
   110881 aatccggaag ttgccaagca taagatactc ttcgacaacc tgactccgct ttatccggaa
   110941 aagaagataa agttagagat tagcccgaat gaagtctcta tgcgtattct ggacttgctc
   111001 gctcctatcg ggaaaggtca gagagctttg attgtgtctc cacccaaagc cggcaagacc
   111061 atactcctgc agaaaatagc caacagcgtc accaccaatc atccggaaat taagttgatt
   111121 gtccttttga ttgacgaaag gccggaagag gttacagata tgaaaaggtc agtcaaaggc
   111181 gaggttataa gctcaacctt tgatgagcca gcagataggc acgtgcaggt ggcggatatg
   111241 gtaattgaga aagccaaaag attggtggag catcagcagg atgtagttat acttctggat
   111301 agcatcaccc gtttagcccg ggcacataat gcggttgtcc ctcacagtgg aaaaatctta
   111361 tccggagggg tagatgccaa tgccctgcaa aggcccaagc gcttcttcgg agcagccagg
   111421 aacatcgaag agggcgggag tttgaccatt gtggctactg ccttaataga gacgggaagc
   111481 aggatggatg acgtcatctt tgaggagttc aagggaaccg gcaatatgga aatggttctg
   111541 gacagaagac tttctgacag gagaatcttc ccagccatag atataaatcg ctccggcaca
   111601 cgcaaagagg agctgttgtt cagcgaagag gaattaaaca aggtctggat tttaagaaga
   111661 ttcttgaatg agatgaatgc ggttgaagct atggaattcc tcctggatag agtgaaaaaa
   111721 accaagaaca acaagcagtt tttggattca atgaaggagt agatagttgc aggcagatta
   111781 ttcttcttaa gtaggggcga ggttgcctcg cccctacttt ttttctgatt ttatgtagga
   111841 cgggataaac ctgccccgat ttttttgcag gcgacattag ccaatgtcgc ctgcattttt
   111901 gctttttgta gttgcccaat ttattgggcg aattttctgc aatgtattcg cgcaggggtg
   111961 gagtttacct ccacccctat tgttcaggcg aagacacggt tcggcaggct caccatctct
   112021 tcgagcctga gcctcagagc cgaaggcctg agcttgtgct tcgacttcgc tcagcaccat
   112081 gagtttatcg aatggtcgaa ggatcccgcc ctttgttttg caggcgacat tccggaatat
   112141 cgcctgcatt tctaatttta ccttgttatt tcaaagaaaa aacataattt tagacagaga
   112201 agttcaaaat agaaagatac tatgcgaaaa cccaaaatcg gcataactat gggagacccg
   112261 gcaggaattg gaccggaggt tttggtcaaa gccctcaagt cggctgaaat aaacaaaaat
   112321 tgtatccctg taattttcgg ctcatacgag ataattttgc aggcagccca aaaatatgcc
   112381 aagaatttaa aaataacaaa acttgaatca ttagaccaaa tcgaagaatc aaaaatcaat
   112441 ctttttgatt gcaccagcat aaatcctaaa caaataaaaa caggaaagat aaattacgct
   112501 tccgccaaaa tggcaattga ttcggtttta gcttgtgcaa aagcttgcag ggatagaaag
   112561 atagatgcta tagttactgc ccctttgtcc aaaaggggtc tgcgtttagc aggatacgat
   112621 tttcccggac ataccgagct tttggcaagt ctcaccaaat cgaaaaatta tgctatgatg
   112681 tttgttggtg acaaactcaa ggttattttg gcaaccactc acctgccatt atccgaagtt
   112741 tccagacact tatctattgg gctcatctta aagaagataa aattggctga ttttagtctg
   112801 aaatcgatat tcaagaagaa aaagccaaga atcggggttt gtgctttaaa tccacacgct
   112861 ggcgaaaatg gtatttttgg aaaagaggaa agacagatta ttgtcccggc aatagattta
   112921 gctcggaaaa agaaaattga agttttaggt ccatatccag ctgattcgat tttttctcct
   112981 gaaatatccg gaaagttcga ctgcgttttg gccatgtacc acgaccaagg tctaatcccg
   113041 ctcaagttgt caggaattgg taattcagtt aatcttacat tgggacttcc ctttgtaaga
   113101 acctcgccgg atcacggaac tgctctggat attgccggca aggggatagc aaatcccaat
   113161 ggaatgattt ttgcaattaa gcttgccata gttttaagac gaatttgatt tttggcagca
   113221 gaacttgttt gccataaaga aaaaacattt ttgtagggac aggtcgcgac ctgtccctac
   113281 tttatgattt tcgacatatt accctattaa atttcgcttg accttcaagg caaaaaaagt
   113341 aatttagatt aagtcgagag tccccacctg ttaagttgga gattaaagcg tttgtgagtt
   113401 cttgtgtcat tcctgtcccg ccaaaggcgg gatgaactcc agcagagcct gtcccgtact
   113461 ggatacggga aatccagagg aaaaaagcca atatggatcc ccaattaaac cctcggggat
   113521 gacaaaacaa gacccgtggg gaactgtgaa agcaccacgc ttgtccttca acatgctcag
   113581 gatcgtgaca gttcgacttc cctcactgtc cctgtcttcg actccgaggt cacttccttc
   113641 gacagtgagc tcaggacagt cgaactgcag actcgaggag agcaaggtcg aagcatagag
   113701 gcgtgggtat ctacgaatag tacaagctaa aggagctgct ttgcttaagg ttacagggct
   113761 cagcaagata tacgataagg atattgcggt tctggaggat ataacctttg aagtcaagag
   113821 aggtgagttt gtagtcatcc tcggaccatc cggagctggt aagaccacta ttttcaggca
   113881 tatttggctg gaggaacttc ccactaaagg ggagatagaa tttgaaaaac taaaaagttc
   113941 aagcataaga aaatctcaaa tccctttctg gagaaagcgg atgggaattg tttttcagga
   114001 tttgaagctt ttaaatgaca gggatgtttt tgagaatgtg gctttgccct tgagaataaa
   114061 gggtaaaaga gaaaaagaga taaccaaaaa gacaatagat atcttaaatc agcttggcct
   114121 cttagttcat cgaagctcat atccttttga attatctgct ggagagaaac agagattggc
   114181 tttagcccgt gctatggtgg cagagccttt aatcattctg gcggatgagc caactgtaca
   114241 tttggatgca gagtccgcaa gtggaatcat cagcttacta agagatgcca atctgggtgg
   114301 caccaccata ctaatggcca ccaatcaaag gggccttttg ggctctttcg cccaccgggt
   114361 aattggttta gaaaaaggga ggatagtttg agttatcgtt attctttcag ggagatgggg
   114421 cttagcttcc taagacagcc aataactttc ctgggttcag ttattaccct ggctttgtcc
   114481 cttttagtgt ttaatacttt tctgttggtc actttgagtc tgaaaaacgt gacagaggag
   114541 tttaaaagta aaatgggaat agaggtctat ttgagagatg atttgaaaaa agaggagata
   114601 tccgcctttt acaataagct tatgggaatg cctgagattg aggaggtaac tttcaaatcc
   114661 aaggatgaag ccttaaagga gatgaagaaa ttctttaagc cggagcttct ggaaggtttg
   114721 aaaacgaacc ctttgcccgc ctccttcttg attaaattaa aaaagccata ccagggtttc
   114781 gctgatatgg agaaggtctc ttcaaagata aaagagtttg gaggggtaga ggatgtggaa
   114841 tacggtgggg aatgggtcaa aaaaatagac caagccattt ttgtttttct cctcattgat
   114901 atattctttg gtttgctcat cgccatactg gtgatgatga ttatagccgg aaatgtcaga
   114961 aatatgatta actccagagt ggaggcgata agcacaatca agcttttagg aggaaatcgg
   115021 gctttcatca gccgtccctt ctttttgcag gggttgatcg aaggtggctt agcagggata
   115081 atcagcctgg gactgctcta tttggtctac aggattttcg tatctaatct tttcaaagta
   115141 gaatttctgc ccctggatct ggctttatca attgttcttt ccgccatcct cttaggaggg
   115201 gtgggcagct tgcttgcgct tagcaaacat atctgattca aatattgctt cgccaactat
   115261 agcaaacgca ttaattacta ttacatggta ggcaatgtgc aattatttca aaatccccct
   115321 tattccccct ttttcaaagg gggagatgcc tttttctcct ctttggaaaa gaggcccgct
   115381 tcgcccgtct cgccgatggc tcgccgaggc gggccgaatc gaggcgaggg ttaggggaga
   115441 tttgattgaa ctcgaataaa tgcgtttgta ttaattagga attggatgtt tgttatatat
   115501 tttacagttt ctacacgaat tccgtgtctt gagaaataac cttgacccat cttgcctgcc
   115561 cggccaggga tgggcttacc gattaacata ttatgcgacc tctatttagt ggacaaaaca
   115621 aaatatgaag tcaaaacgcc tttttgtttt cttgttttta ttgctccttt tttcatttcc
   115681 acaagctcaa gcccaaaaag gtgaagccgg tgcaaaaaaa caacaggagg agcttaacaa
   115741 cataagaaaa gagctggaat ataagaggaa aaaacttaag gagctaaaaa acaaggaggg
   115801 agcagctctg actgaactgc aaaatctcca agaggagctg gagctgatgg agcagttgat
   115861 taaaaaactg gatacaagac tgggcaatgt gaaaatagag ctcaagaggg aaaaccaaga
   115921 attagattcg attcgagtgg atttagagaa gagaaggaag cttttggctg gacgcataag
   115981 aggaatatat aaattctggc ggttttatca gtatgaggcg atactgggaa gcagttcagt
   116041 agctgacctt ctcggccggc tgaagtatct acagttaata gctaagcagg acaatgaact
   116101 catagatggg tttctaaagg acaaagagaa attggagaaa agcaagaaaa actcagaagc
   116161 caagaaaaat gaactttacg gcctcaaaaa agaaaaggaa gatgaagaga agagtcgaac
   116221 cctggagaaa caaaagaaag agaaagtctt aaataagata aaaagcgaga agaaagctta
   116281 ccttcaggca gtaaaagagc tcgagggaag cgccagaaaa atagaagcat ttctcacaaa
   116341 atacgaagag gagaggaaaa aagaggaatc agaacaggac ctgttcaaac tcctgaaagg
   116401 gagactggat tggccggtta aaggagaggt gatttccaat ttcggggaac aggtgcaccc
   116461 ggtcttcaag actgtaacct tcaatcaagg catagacata caagctaacc cgggcacaga
   116521 agtcaaggca gtggaagaag gtaaagttat atactcttct tatttgagag gtcgggggga
   116581 atttttaatc ttacagcatg aatatggcta ttacaccata tatgcggatt taggagatgt
   116641 cttagtggat gaaggagaag aagtaatcaa ggggcagaca attgctcgag taggtgaact
   116701 tagtcttttt tcaagtttcg gcttgcactt tgaactgaga aaggggaaga aacaactcga
   116761 ccctttggag tggcttaaag aatgaccttc tcgaaagtga ttggacagga tttggtcaag
   116821 caggttttga atagagcttt ggaaagggat aagttagctg gtagctatct tttctttgga
   116881 ccagagggtg tgggtaaatg ggcaactgcc ctagaggtcg ccaaacgttt gaattgccaa
   116941 aaggggaatt tgggtccttg cgggaattgt gccagctgta aaaagataac caggttatcc
   117001 catgcggata ttaccctctt gtttcctctg cctgcggtga aaaaggagga ggaaaaagga
   117061 aaattcaggg aagagttcaa aaatgaaaag atagaagagc cctattctat tatgtgtttt
   117121 gacaagccga gcaacatagc agttgaagag ataagagaga tgcaaaggga gttgagccta
   117181 aagccctatg aagccaagag aagagtggtg atagtctatc aggcggagaa aatggcagtg
   117241 gctggaggaa attcccttct gaaaagtctg gaggaaccac ctttggatgc ggtcttgatt
   117301 ctgacttctt ccgagccgag caaactgttg cccaccatac tgtcgcgttg tcaaaagata
   117361 aggttttcgc ctttagcgga tgccttgata gaggattact tgattaccaa ctataagctc
   117421 agtagccagc aggcacgttt ttgctccaag ctctccaggg gaagcttggg taaagccctg
   117481 agtcttatgg atagtgagcg tagcgagatc agggaagatg gcgtgaattt gctgcgttgc
   117541 gctctaaagg aggagctggt ttcttcagta gaattagttg gtgggcttct aaggaaatgg
   117601 gatagggatt cgatcttaga gatgtttgat tttttgttca cctttttgag agacatttac
   117661 ttatatttag aagaggaggg agatgatgat ttgctcaacc tggatctcaa ggaggagata
   117721 atcaagctgg caggtatctt aaaagaagga gagttggttg aggaggggat gggtttgata
   117781 aaccggacca aagaagattg cataaaaagg aacgccaatt tagagctttc ccttttggct
   117841 ttggttttga gttggaggag ctacataagg aggtagttgt gtcggaagtt tacttagttc
   117901 agtttaaggg atcacgaaaa ggatactact ataatccaaa ctatctgaat ctcaagttag
   117961 aggattacgt tattgtgcaa gtggaaaggg gtgaggatat ggggagggta gttaagaaaa
   118021 gcagcgaaga gctcatatta aatgaagaga agaagccatc caacatcctg cataaagcca
   118081 ccccggatga cattgaaagg ctaaaggcta atcgagagaa ggaagaggaa gcgctgaaag
   118141 agtgcgagag gatgatcggg gcgcgcaact tggagatgaa attagtggac gccgagttcc
   118201 agttcgactg caacaagata accttctatt tcaccgcaga caagagaatc gatttccgtg
   118261 aattagtcaa ggagttggct tcagtctaca agacgcgcat agagttaagg cagattggag
   118321 taagggatga agcaaggtgg attgggggtt atggatgctg cggtttaaag gtatgttgct
   118381 ccaccttcat aaagggtttt gagccgatat caacccaatt agccaaagag cagaatctct
   118441 ccttaaatcc tgctaagata tccggaaact gcggaagact tttgtgttgt cttttgtatg
   118501 agaaggattt ttatcatgag gcattctcct cctaccctaa ggtcggctcc aaatatcaga
   118561 cggataaaga agaagggata gttgataacg ttaaccccct tggcgggtat atcgtggtga
   118621 ggcaccagga tgagaccgaa gagaaggtca gtttgttgga tataagaaaa ggacagaaga
   118681 agaaacacct cccccgcgga accaaacaga aaagcgaagt tggttgaaga atctattttt
   118741 cagaccaaat ttgccttggc agttattatc ctgagctggt tttaagggtc tcaagagatg
   118801 ctgaaacgag ttcagcatga caagaaggaa gagatgctga aacaagcctg tcctggcgtc
   118861 aggccacggt tcagcatgac atatcgggac cctgcaggaa taagggtatc tgaaaacccg
   118921 aatcaaataa gggttttttg attttgtgaa aggcacggat ttatataaaa aaaggagaga
   118981 aattggataa gagatacctc ataaccactc ctatttatta tgtgaatgac caaccacaca
   119041 ttgggcatgc ctacactact attgcggctg atgttctggc tcggtttcac agaatcaatc
   119101 aagaggaggt tttctttcta accggtacag atgagcacgg ctcaaaagtg gctgagactg
   119161 ccaaacgggc cggcaaaacc cctcaggagt tgtgcgacat taatcataaa ttctttgtcc
   119221 aggcctggaa gaaccttgat attgcttatg actacttcgt gaggaccact gaccacagac
   119281 atgaaaaggc agtttgggcc ctcctcgaaa aaatgaagaa ctcaaagaca ccagataaaa
   119341 aagaggtaat ctatcctggt caatatgagg gtttatattg tgttggctgc gagaaattca
   119401 tcactgaaaa ggatttacaa aatgggattt gtccggagca tcccaatatg aagatagaga
   119461 gattatccga gaagaattac tttttcagac tttcctctta tttaggcaaa cttaaagacc
   119521 ttattggaga ggataagata aagatactgc ccgaggaaag aaaaaaggaa accctgggtt
   119581 tgataaagca gaaaatcgat gacttttcca tatcccgtga aaaggttgcc tggggagtga
   119641 gcttaccctt tgacccgtct caaaaagctt atgtgtgggt ggatgccctg cccaattaca
   119701 tctccgccat aggatatggg gataacctgg aggagtttaa aaagtggtgg aaagaggcag
   119761 ttgtgcttca tctcatcggc aaagatatct taaagtttca cgccatctat tggccagcca
   119821 tgcttttgtc tgttggtgaa aggccaccgg ataccatctt cgcccatggg tattttacag
   119881 tagacaacag aaaaatggga aaatctttgg gaaatgtaat tgaccccaat ttcttagtga
   119941 caagtttcgg cagcgatgcc gcgcgctatc ttcttttgac ccaattcccc tttggtcagg
   120001 acggggatgt gcagctgagc cgctttaatg aaaagtacaa ttccgattta gccaatgatc
   120061 tgggaaattt ggtcagtcgg gttttaaaaa tgatacagaa ttattgtgat ggcaaaattc
   120121 ctttggcttc gacttaccag ggaatagacc gaaagctcat agaccttgcc acagccattc
   120181 cttccacggt gatggagaac gtaaatgcta tcaatctgaa tggcgccatt gaacagataa
   120241 tgaacttagt gaaggaagct aaccgctatg tggaaagcca atcaccctgg aatttagcca
   120301 aaagcggcca aaaggagagg ttgggaactg tgctttatgt ttcttctgaa gttttgagga
   120361 taataagtgc attgttctat ccggtgctgc cgcaaaaaat caccggtctg agagctcttt
   120421 tgggatttcc taaaaataag ctaattccta atattaaaga ggaaagcaaa tggggattat
   120481 tgaaagcagg caccgagata aaagaattcg agtctctttt ccctcgtata ttagaggaaa
   120541 aggcgccaaa ggaggaagct ggtgtggaag agataaattt agaggagttc tctaaaatag
   120601 atttaagggt ggctaatgta ttatctgcgg aaaaagtaag cggggcggac aagcttttga
   120661 aactggagat aaagataggt gaccagaaaa agcagatagt agctggaatc gcccaatatt
   120721 attcacccca ggatttaata ggccgaaaga tagtggtggt agctaatctt aagcccgcta
   120781 aaatcagggg gattgagtcg aatggtatgc ttctggctgc caaggatggc gagtctttgg
   120841 tcctggtgac cacagataaa gatattaaag atggggcaaa gataaattga aaaaatgatt
   120901 gatactcacg cccaccttga ttcccctcaa ttcgacaaag acagagagaa agtaattcag
   120961 gaagctaaat cctgcggagt ggaaagaata ctcaatgtag gagtggacca agaaagcagc
   121021 cagaaatctg ttgacttagc tcaaaggtat ccccaaatat atgccacagt tggattccat
   121081 ccccacgatg ccaagaaatt caatcaatcg agtttggaag aattaaggag attggcacaa
   121141 caccagaaag ttgttgcctt aggtgagatg gggttggatt tttatcgcaa tctctctcca
   121201 gctgaaatac aaaaaagtgt ttttatctct caattggaat tggctggaga gctggatatg
   121261 ccggtggtgg tgcacattcg caatgcttat ccacaaagtc tggatatttt ggaacaaaga
   121321 aaaagtgtca aaggggtatt gcattgtttt gaaggagata ttcaagaagc taaaagagga
   121381 ctggatttgg gattttacat ctcctttaat ggaagaataa cctatcacaa cccttccttg
   121441 attgatttgg tcaaaaaact tcccttagat agaatattag ttgagactga cagcccctat
   121501 ctcccacctc atcctcataa agggcagaga aaccaacctg cctgggtgga gttggtcata
   121561 agagagattg cccaaatcaa gggcaaattg gtttttgaag aacttgagaa aatcactact
   121621 ataaatgctt gtaacctctt tggttggaaa caagatgagg agtaaaacaa aggaaaatca
   121681 aacttccaag cccaggaaga gtctgggaca gaatttcctc ataaataaat gggcggcgtt
   121741 aagcatagtg aagagtttaa acctctccga ttcagatatt gtcctcgaaa tcggagcagg
   121801 caaaggggct ttgactggat atcttttgga aagatgcaag aaggttgtag cagtggagat
   121861 agataagaga tggtgtgaat atcttgaaga taagttccga ggttttgaga atcttatcat
   121921 cctcaaccag gatatactaa agttggactt gaagaaatat gcacaaggga aaaagatgaa
   121981 aatagtgggg aatatcccct atcatttgac cgcccccgtg atctcttacc tgatagagaa
   122041 taagaacctg atatctcaag ccctcctcac tgtccaaagg gaggtagcca aaaggatctg
   122101 tgccaagccg ggaagtggcg actggtcact tctatcaatc ttcacacagc ttcacgcttg
   122161 tactaaacta ctttttgttt tggaacccaa ctggtttttc ccccaaccaa aagtgcattc
   122221 cgcagtggtt gagttggttt tcctaaatgg tttggcagtc ggggtagaga atatcgatta
   122281 tttttttaag atagccaaaa tgatatttca gcagaagagg aaaatgataa tcaattcttt
   122341 gacatccgga ctggatttat ccaaaagcga gatcaaagag aaatttgaag atattggcat
   122401 agatccgagt ttaagacctc aggagttagg tctggataaa ttagccatta taagttctgt
   122461 aatgataggc tctaccaata aaccttagga ggtaattatg cctaaattaa gtgttaatgt
   122521 tgaccatgtg gcaactttaa gggaggcgcg gaaagtggac cagcccgacc cggtggttgc
   122581 cgctattatg gcagaattag ctggagcaga tgggataact gttcatttgc gcaaggacag
   122641 aaggcatatc aacgatagag acctggggat attaagggag gtgataaaaa gttctctcaa
   122701 tctggagatg gccgcaaccg aggaaatggt ggagcgggcg attgaaatta agccggatat
   122761 ggtaaccctg gtgccggaga aaaaagagga gctcaccaca gaaggggggt tggatttgca
   122821 ggcgaatttc gaaaacctct cacaaagagt atccaaactg cagataaatg agatgttcgt
   122881 tagcttattt gtcaatccgg acatggaaag catcaagcag gcagccaaac tgcgggctga
   122941 ttttgtggaa atcaatacag atgcctattc acgtgccaaa aaattgagcc aaaaaataga
   123001 agaactggag aggattgaga ggatagtcgg tttggcctat aagttgggat taagaataaa
   123061 tgccgggcat ggcttgaact ataccaacat ctcagacctg gtaatgatcg agcatatcga
   123121 ggagttctcc atcggtcatg ctattgtggc acgagcggtt ctggttggct tcgagagagc
   123181 taccagagag atgctagacc tagtcaaaaa gaggggttga cttttaagtt gagtttctta
   123241 tcttatatga aaatttaaac tgcctgactg tgaattatga aacttgacaa gatattcgcc
   123301 ccggtaaaaa agcaactagt ttcctttgat aaaaccttag aggaggcgct ttcctctgat
   123361 tcctctttga tctttgaagt ggtcaagcac attttggatg ggaaagggaa aagattgcgg
   123421 cccagtttgg tttttctgtg ttggggtatg tgcggaggta agagtaagaa tgccactaag
   123481 gtggcagtgg ctattgactt gattcacaat gccaccttgc tgcatgatga tgtcatcgac
   123541 cagtcaccaa ctcgccgcgg taaagcctca gtcaactgca aatggaataa cttagtctca
   123601 attctgatgg gagattacct tttgtccaaa gccttcaaga taatgaacca gaccgagtct
   123661 ttggagttga tgaatgatat gtccagagcc accgagaatt tgaccatagg agaattgagg
   123721 caggttcagg aggctagaaa ttatcaactt aatgagaaga cctatcttaa gatgattgag
   123781 gataagaccg cctctttgtt ttcagcatct tgtatgggtg gagggataac ggccggggca
   123841 aaagaggagc aaaggaaaca tcttagtgag tacggtcgca atctgggcat tactttccag
   123901 atcacagatg acgtgttaga tttcataggg gaatcaaaaa agaccggcaa aggattgggg
   123961 aatgacctca aggagggtaa agtcactttg cctttaatct acaccctgag gcaagctgag
   124021 ggtgccacca aaagaaggat aatccggctt ctggaaaaag atttcaatta tcaggatttc
   124081 gtcaagatcc tctctttagt cagggagtat gggggaatag attatgccaa atggagagct
   124141 gagatctttg ggcaaaaagc tttaagccat cttgctggat tcaaaaattc tccctataaa
   124201 cagtccttaa tggatatggc tgattttgca gtcaatcggg ataggtaata ctatagcaaa
   124261 cgcattaatt actgttatat ggtaagcaat atgcaattac tttttgaatc ccccttattc
   124321 cccctttttc aaagggggag gtgatttttc ccctctttgg aaaagagggg ttaggggaga
   124381 tttgattgaa ctcgaataaa tgcgtttgta ttaatacaga tcctagttat catattaacc
   124441 actgaaaacc aatcagtggt tttttgtttt ttatgacggt ttgtggtttt ttcccttgaa
   124501 aaaagataga acatagttta gattagtctg aagaccaaga tcaagaaagg agaaaaagat
   124561 gccacagata gtttcctgtt ttgcgcggga gatactggat tctagaggta atcccacagt
   124621 agaggtggat gtgattctgg agggaggggc ttggggaaga gcagcagttc cttcaggtgc
   124681 ttccactggt gagcatgaag ctttggaatt aagggatggc gataaaacca gatacttagg
   124741 aaagggagtg ctcaaggcgg taaataatgt taatcaaatc atagcagcgg agcttgtaaa
   124801 acagggtttg gatgccaccc agcaggtggg aatagacaag ttcttaattg ctctggatgg
   124861 aaccgaggat aaaagcaaac taggtgcaaa tgcgattctg ggagtctcct tagcagtttg
   124921 caaagcggct gcatattacc ttggtttgcc cttgtatcaa tatctgggtg gagttaatgc
   124981 caagattttg cccattcctc agatgaatat cataaatggt gggaaacacg cagataacaa
   125041 cgtagatttg caggagttta tgattatgcc aataggggca agctctttta gggaagccct
   125101 aagaatggga gcggagactt tccataattt gaaaagcgtg ctcaaagaga aaaactacaa
   125161 taccgcagtg ggagatgaag gaggattcgc tccagatttg aagtcgaatg aagaagccat
   125221 acaggtcatc cttaaaggaa tagaaaaggc aggttataag ccgggcgaag atatctacat
   125281 tgccctggat ccagccgctt ccgagttctt cgataggaat tcaaaaacat acaatttgaa
   125341 agcggagaat aagaaattca gttcttcaga aatgataaga tattatgaga gtctcatcaa
   125401 gaaatatccc atcatctcca ttgaggatgg actagcggaa gacgattggg agggatgggt
   125461 tgagttcacc aagaaattgg gtgataagat tcaactggtt ggagatgacc tctatgttac
   125521 caacatcaaa agattagcca aggggattga actgggggca accaattcaa ttttaataaa
   125581 gcttaatcag attgggactt taaccgagac tctggatgca attgctatgg ctaagcaggc
   125641 agggttcact gcagtggtat cccatcgctc tggagagact gaagatacaa ccatatccga
   125701 tgtggtggtg ggagccaatt gcgggcagat aaaaagcggc tccacttctc gcacagacag
   125761 gatctgcaaa tacaatcagc ttttgagaat tgaagaggag ttgggagatc aggcccaatt
   125821 tctgggtaaa aaagcattct ataatctgaa atgaaaaaag ggaaattaat agctatctta
   125881 gacaatcgat tcttcgctcg ggtgaagaaa gaggcaacca agaaaaaggg aaaactcaga
   125941 gccattcttt ttttgatggc tctgctttta ttagggtatc tctacatggc tggagattac
   126001 ggctttgtcc gcatattttc gttgtggaag gaaaagaaaa gtttggtgat ggagataaag
   126061 aaaatagaag ctaagagcat tgatttgaaa gttgaaaaac aaaaattgac ctctgatcat
   126121 gattatattg agcgaattgc ccgcgaaaga tatggcatgg tgaaaaaagg ggaaaaactt
   126181 tataagttcg tgcagaaaga gcccaaggac ctgtccagat gagcatctgc aaagcttctg
   126241 ccttagtttt gaaaagcatc aacctgagag aaagttcccg catcgttact ctttactcca
   126301 aggaattcgg aaagatgaag gtggtggcaa aggggatgct actgccccag agcagatttg
   126361 gtggaagttt ggagaatttc tcagaaatca atatcgtttt ctacaaaaga gagaataccg
   126421 agctttacat tctttcccag gttgatctgg ttaatccttt tcacaaactc tgtctggatt
   126481 tggataggtt cgccatcgcc tcgaccggaa tggagcttct ggataaactg gtttcctggg
   126541 aggagtcctc gtcaaagatt tttgacttga gcttggattt cttgagccag atcgaatcca
   126601 ccgggaaaag caatctcaaa aaaatcttat tgggttatgt gctcaagcta gtgaccattt
   126661 taggatacaa acccgagtta gaggattgtg tagcctgtga taaaaaaatc aaagacagat
   126721 tcattttttt taacccggaa aggggaggat tggtttgtcc ctcctgcttg atagatgacg
   126781 gcttctacat taagttcaca agagattcga tagaaaaggc aagccagttg aggagattgt
   126841 ccttgaaaag tgctggcaag atcaagatgt ctgaaagcca gtcagatgag gttctctgtg
   126901 gaattctgga ctttttggat tttcatgttg gtcgaggaaa ggatttgaag tccatggagt
   126961 tctttaaaag tataaattcg ggaggcataa gtgaatcaga aggataacgt aatggagaaa
   127021 atagtttcac tctgtaaaag aagggggttt gtctttcagt ccagcgagat ctatggtgga
   127081 ataaatagtt gctgggacta tggtcctctg ggagttgagc ttaaaagaaa catcaaggat
   127141 tattggtgga gatgtatgac tcaactaaga aatgatgtgg ttggaatcga tgccagcatc
   127201 attatgcatc ccagagtttg ggaaacatcc gggcatgtgg caggctttgt tgaccctttg
   127261 gttgattgca ggaaatgtaa gcaaagattc aaggctgacc agttgaaaga aaacaagtgc
   127321 cctgaatgcg gaggggagtt gacttctgcc cgtcaattca acttaatgtt caaaactcat
   127381 ttgggaccgg tggaggatga tgcctccatc gcttatttaa ggcctgagac cgcacaagga
   127441 atttatgtca atttcttaaa cgtgctcggt ccttcccgtc agaaaattcc ttttggcatt
   127501 gcccagatag gtaaagcttt tcgcaatgaa atctctccag gaaattttgt cttccgctcg
   127561 agggaatttg agcagatgga gatgcagtac tttgtccatc ccaaagagga tgtgaaatgg
   127621 ttagaatatt ggaaaaacga aaggatgaat tggtatctgg gtttgggtgt aagagaagag
   127681 aatttaaaac ttcatcagca cactaaaaag gaattggctt tttatgccaa ggatgcgttt
   127741 gacatagaat acaatttccc ttttggctgg caggagatag aagggattca caatcgaact
   127801 gactttgacc tttcccgtca tagccaagcc actggaaaag acctctccta ttttgatgac
   127861 cagaccaaag aaaaatatat cccctacata attgaaactt cagccggagt ggataggagc
   127921 ttgcttgtct ttttagtgga tgcttattac gaggaggaag taaaaggtga aaaaagagta
   127981 gccctgcggc ttgaccccaa aatcgcaccg ataaaagcgg caattctacc tttggtgaaa
   128041 agggataata tgccagagat tgctcaagat atatataaaa agttgaaacc acattttaat
   128101 gtcttctatg atgaaggtgg ggcagttggc agacgctatc gtcgtcagga cgaaatcggc
   128161 acaccttttt gcataaccat tgattcccaa accttacaag acaacaccgt aaccttgcgt
   128221 gaaagggatt cgatggagca ggtgagagtt aaaattgatg atttacgaga agagttggag
   128281 aggaggattt gggggtaggc taaaaattaa atagaaaggg ggttttatga agatttgtcc
   128341 gagatgtggg aaagaattag aggataatgc tattgtttgc aagtattgca gtctcggttt
   128401 tggattcgga attctttggg cgggtttggc aattatggtc ggagttttag gcattttagg
   128461 accatttatt gcatccactt tattgcgggc tggtccgggt ctcattgaga taatagccct
   128521 ggcggttatc tccgttattt tgattgtggc tggaatccta gttttgaaaa aaagaaagct
   128581 cggcttatat atagtatttg tactgatacc tattgacatt atatcctctt tcatagctct
   128641 cttaacagga ggcgattttg tttggttttt gatatctttt attctcggtt cgctgtggct
   128701 ttggtatttt tatagaagaa ggtatatgtt taagtaaccg acatttcgga atgtcgccta
   128761 cgggtttgtt tttgtaggga cagaacagtg ttctgtccgc atcccttccg cacggacagg
   128821 tcgcgacctg tctctacgtt aaatattcaa tattgaggag aaaattatga aaaagaaaca
   128881 ttcgataatc tttctggttg taattttggc aatatttctt tttatctttc ccgtcaccaa
   128941 tggtcaaaac gaaatacctt tgcccaatcc cactctaaaa ggtcaaaaaa gttttgaaga
   129001 agttctctcc actcgccgct cgagaagaag ctttaaagag ggggctatag ctttgaaaga
   129061 actttcccaa attctttggg cgagccaagg aataacttcg aaagatggca aaagaaccgc
   129121 accttcagcc ggggcattgt atcctttgga aatctatgtt gtagtgggtg aagttgaggg
   129181 cttggagccg gggatttatc attatgatgc acccaatcac aagttaataa gagtaattga
   129241 caaagataaa agaagcgaat tgaatttggc tactggggga ggcaggagaa atatagaaaa
   129301 tactccagtc agtattataa taaccgccat atatggaaga actacaaaga aatacgacca
   129361 aagaggaata agatatgttc acatagaagc tggggcggtt tgccagaata tctatctgca
   129421 agtggaaagc ctgaatcttt ccacttttgt gatgggagca tttgatgacg ccgaagtcaa
   129481 aaaagtgctg gcactgaacc agaacggttc agtgctgggg ggtgaagttg agcccatttt
   129541 gattatgcct atcggaaaaa gaatttaata acaaaaccca ccataagtta tctttcctgc
   129601 ccctttttgt ttgacaaaat ttcaaatctc ccctattata taactaatta agggcttatt
   129661 gaagaagtaa gagatttgtc attgcgagtc ccgattttat cggggcgaag caatctcgat
   129721 tcgaaaaaga gattgcttcg tttcccgcct gtggcaggat cctcgcaatg accgaaaacg
   129781 gcgttcggca ataacctctt aatccaccta aaaatagcta accttatgga agtcaaaaac
   129841 tcttttgtaa aaaaggtcga aaagaccatc cagcactaca aaatgctgca acagggcgat
   129901 aaagttctgg ctgcggtttc aggtggaccg gactcagttg ccctcttgca tactctaaat
   129961 ctgcttaaag atatatttaa gattaagctt atagtgggac atataaatca ccagcttaga
   130021 gctagcgagt cagataaaga tgaaaaattc gtcaaagctt tatctgaaaa actgaattta
   130081 aaatgttttt ccaaatcaat tgaagttaaa aaaacagccc agaagaagaa aatatcaatt
   130141 gaggattgtg cccgtcaaat gcgttattct gctttagaga gcatagccaa taaaaccaaa
   130201 gccgacaaag ttgctttagg gcataactta gatgaccagg ctgaaaccat cctgattcgc
   130261 ttaatccgag gctcgggacc attgggtttg tgcggcatac cggcagtcag aggaaaactc
   130321 attcgacctc ttattcaaac gaaaagagag gagatagaga atttcctgaa agataatctc
   130381 tgggagtgga gagttgattg gtctaacttc aatatggatt atttaagaaa tagggtcagg
   130441 ttgaaacttt tgtccttaat caaaaaggag ttcaatccca acatagttga ggttttagcc
   130501 cggacagctc aacttttgtc agaagagaaa gattatctta cgaatcagat tgaaaaagca
   130561 ttcagggatg cagtttcaag ccaagatagc gagaaaatag tgcttgattt aaaaaaactg
   130621 attggttatg atatatattt aaagacaaat gtcatccgct tagctttcaa tcgcttaagc
   130681 ggcgatctgg attttccatc cttttccata gttcaaagag cgactggtct cctggaggaa
   130741 agaaaagcgg gaaagagggt ggagttgggt aagaacatct gggttgaagt ctcatctgat
   130801 gccctgatag tgtataaatc aagaaaacca gctaaggcgc attgggtcaa acttccgggc
   130861 aaactcgaaa ttgataatct aaagttaaaa tcagaggtca ttcagcgacc taagctgccc
   130921 aaaaagataa agtcaaacag cccgtggatc gcctatctgg attgggataa gttatcttct
   130981 ccgtttgggt taaggcacag gaaaaataag gattggttta tacctttggg gcttgggggc
   131041 agcaaaacct tagctgattt cttaattgac ttgaaagttc cgagaatgga aagggatgag
   131101 attttccttt taacttcaaa gaacagaatc gcctgggtga ttggacagag aatcgacgat
   131161 agatttaaag tcaactccaa taccaaagag gttttaaaaa tagaagcgtt tcttgaaaac
   131221 taaactaaaa gtgatggaaa aaataaaagt aaagaaaata atatctaaat cccaaattca
   131281 aaaaagagta aagaaactat cccaaacgat ttcgagagat tatatcaaaa gagacctgat
   131341 tttggttggc atcctgaaag ggagtttcgt attcttagcg gacttgacca gaaaccttaa
   131401 aattcatcat caggttgatt tcatctcggt gacaagttat ggttcttcca cacgtgccag
   131461 cggcattgtg aggatactta aagacttaag cgtcaatatt caaaacaggg atgtattgat
   131521 tgtggaagac atagttgatt ccggtttaac tttgaactat atcataaaga atttcaaaac
   131581 tcgtcaaccc aagagcctaa aggtgataac tctcctgaat aaaaaaaaga aaagagaggt
   131641 gtctatacct ttaaaatata tcggctttga tatcccggat gagttcgtgg tgggatacgg
   131701 gctggatttg aatgagagat tcagaaatct acctttcata gccaagatag aaaaatagag
   131761 ggtgatgcat atgcaaaaag atgataagaa gataagattg gacagaaaag aagatgacag
   131821 gagaagccca agggaccagg agaagaatca gttccagtgg aaaagatcag gcagaactct
   131881 gatcttctgg atcgcgcttt tggttttatc tatcctcttg ttccaatact actcccgtgg
   131941 ggggaaagat ataattgata tcagctatag tgagtttcaa caagagctcc tccagtccaa
   132001 tatcaagagc gtcctggtaa ttgagaaaaa tgttgaagga gagtttgtaa aagagataag
   132061 caaatctgat aggggccgaa cccaaagcta tcctcatttc agaacatatc tgcccgtgga
   132121 gagtcaggag ctgatcagcc agatgcagca aaaaggtgta cagatagaag ctcaggccgc
   132181 cagcgtgaac tggtctggaa tgcttttaac ttcccttccc tggattttgc ttttagcctt
   132241 cttttggttt ttcctctttc gtcagatgca gggaagctcc aaaggaattt tcagttttgg
   132301 gaaaagcaga gccaggcttt taactggaga taggcccaag gtgacttttg aggatgtggc
   132361 aggggcggac gaagccaagc aggagttgca ggagataata gagttcttaa aagagccggg
   132421 caaattccag aagttgggcg ggaagattcc caaaggagct ttacttttgg gagcgccggg
   132481 cacaggtaag accttgttgg ctaaggcagt ggcaggagaa gcggaagtgc cgttcttcag
   132541 catgagcggt tcagattttg tggagatgtt tgtgggagtg ggcgcctcgc gggtgaggga
   132601 ccttttcgag cagggaaaga aaaatgcacc ctgcatcatc ttcattgatg agatagatgc
   132661 agtgggaagg cataggggag cgggtttggg tggaggtcat gatgaaagag agcagacctt
   132721 aaatcaactt ctagtggaga tggatggatt tgaatcaaat gagggggtaa ttctcttggc
   132781 tgccacaaat cgtccggatg tgttggatcc agccttactt aggcccggca ggtttgacag
   132841 gcagataata gttgatattc cagatgtcaa gggaagagag gggattctta aagttcatac
   132901 gaaaaagata aaattggggg agggggtgga cctttcgatc ttagctcgag gcactcccgg
   132961 tttatgcggc gcggacctgg ctaacatggt caacgaggca gcccttttag ctgctcgtca
   133021 taaccgggat ggagtcacta tacaggactt cgaagatgcc agggacaagg ttatgatggg
   133081 ggttgaaaga aggagcctgg tgataagcga ggaggagaag aaaaccacag cctatcatga
   133141 atcaggtcat gccctggtag ccaagctcat tcccggctca gacccggtgc ataagatgac
   133201 cataatccct cggggtttag ctttgggctt aacccattat cttcccgtag atgagaggca
   133261 tacccattcc aaggaatatt tggagaataa attagcccag cttttaggtg gaagggtggc
   133321 tgagaaattg gtattcaatc aatataccac cggcgcgggt aatgatctgg aaagggcaac
   133381 ggaattggct cggaagatgg tttgcgaatg ggggatgagt gagaagttgg gacctctgac
   133441 ctttggtaag aaagaagaag agatattttt aggtagagag atagccaagc atcgagatta
   133501 cagcgaggag accgccaaac agatagatgc tgaggtgagg aagatagtgg aggaggcaga
   133561 agctaaagcc actaatctcc ttcagggaaa tcaagagaaa ttgcatactt tagccaaggc
   133621 gcttgtggaa aaagagatct tggatggtga gcaaattgat agaatcctca aaggagagaa
   133681 gctggaacca ggaaaatcca ataatgaaaa tgaaaacaag aaagataagc cgaaggaaaa
   133741 ttgaggaagg gataagactg gctttggagg gggcagggga aaaccctcaa agggaaggtc
   133801 taaaagatac tcctcgaaga gtggcagatt tatacgaaga gatttttgcc ggattgggaa
   133861 tcgaccccca aaaagagctt aaaatttata atactcctaa tcaggatgag atgataattg
   133921 ccagggatat acctttttat tccatgtgcg agcatcacct tctccccttc ttcggcaaag
   133981 tccatattgc ctatattcct cagaaaaata aagtaaccgg cttttcgcac ttagcccaag
   134041 tagttgaaat tatagccaaa aggcctcaat tgcaggagag gttaagttcc cagatagctg
   134101 atgttttaat gcgaggttta aaagccaaag gggttctggt gataattgag gcggaacagt
   134161 tgtgtttaac gatgcggggc ttaaaaaagc aaggagtttc aactatcacc tcagccataa
   134221 ggggtgtaat gaagaagtcc aatatccggg ctgaggcttt gggtttgata aagagtgaac
   134281 ttccccgccc aaagagcggg catccaaaac atctcctccc ccttgagggg gaggactaag
   134341 ttgaaaatct cgccaaaggc ggagagaggg tcagaatcat cttcgctatc cccctcaccc
   134401 cttcgcttcg ctttagggca ggctctaacc ctctccccca agggagaggg aacttataga
   134461 aatgtcattc atccccccta cgggggtatt ctgacttgtc tttcataaat aaataaggag
   134521 gtcgatttca agctgaagct ctgcactaaa gaaatagacc tgtcctctcg cactcatatt
   134581 atgggaatac tcaatgttac cacggattcc ttttcggatg ggggtaaatt tttcaaattt
   134641 gaggatgcca aaaggcaggc tttaaaaatg gcagaggaag gggcagatat catcgacatt
   134701 ggtggggagt caactcgacc tggctcagaa gctgttccct tagaagagga attggaaagg
   134761 gttattcctt tgatagagga tttggtcaaa agcacaagca tccccatttc catagatacc
   134821 tataaatcgg aagttgccca aagagctctg gatgctggtg cggagataat aaacgacatc
   134881 agcgccttaa ggttcgacca aaatatggca gatatggcag ccggatatga tgcaggaata
   134941 gttttgatgc acattcaggg gactcccaag aatatgcagg agaatccaca ttatgataat
   135001 ttaattgagg aaataaaaac ttatctgaaa gaaagcataa aattagcgga acaagccgga
   135061 gtaaaaaaag ataagatagt aatcgacccg ggcatcggtt ttgggaaaaa agtagaacat
   135121 aacctgaata tattaagaaa cctaaaagag tttggggaat tgggcaagcc aataatggtg
   135181 ggcacatcaa ggaagtcatt tatcggcaaa ttacttgacc ttccggtgga gcaaagatta
   135241 gaagggagct tagccggtct ggctgtgagc ataatgaatg gtgcaaacct agtcagggtg
   135301 catgatgtca aagaaagtct aagagtagtt cgcctggtgg atacgattat aagaaattaa
   135361 aaattaaaaa agacaaatta aaaaattatg gagctttttc aggttggttt tatcaaattc
   135421 ggaatagtgg acttgatcga tgtggcgatt gtctggtttt tgttctatcg gcttttaatc
   135481 ttgatgaaag ggactcgttc ggttcaaatc gtgattggct tgtttttggt cattttagtc
   135541 tcatttatcg ctttctggtt tcaattggag ggactgaaat ggctcatcag caatatcgcc
   135601 acagttggaa tcatagtctt agtcatagtc ttccagccgg aattaagagg agcgctcgct
   135661 cagataggac acaacaaatt catccgcatg ttcttccgct atgaaaaaga gaaaactctg
   135721 gatgaggtgg tgaggggcgc catcaaatta gcggaactgc gctatggagc cttaatcgtt
   135781 ttggaaagag acatcggctt aaaggacttc gctgagaccg gcaaggaact gggcgctcag
   135841 gtttcaagtg agcttttagt cacccttttc accccttata ctcctttgca tgacggggca
   135901 gccatcattt ccggagagaa tatcgtagca gttgcctgca ctcttccttt aagccaaaac
   135961 ccgcaataca tcaaactata cgggatgagg catcgtgccg ccgtgggaat aaccgaggag
   136021 tcagatgcgg tagtggttgc tgtttccgag gaaaccggta acatatcagt tgccttcgga
   136081 ggaaggttgc agaagaatat cgacaagagc gtgttgaagg atatgctatt gggactgatg
   136141 cgagaaagtt gaattagcga tgtttattga ttacgttgag attctggcac aagcgggtga
   136201 tggcggaaat ggttgtgtga gtttcagacg ggaaaaatat gttcccaagg gtggaccaga
   136261 tggcggggat gggggggatg gcggaaatgt aataataaaa gtcaatccca acctttctac
   136321 ccttatagac ttgagatacc gaaaggtcta taaagcccag acaggaggaa acggagaggg
   136381 aggcaacagg tcgggcatca gaggaaaaga tataatcata aatgttcctg gggggacgat
   136441 aataaaagat ttacaggaaa ataaaatatt agctgatctg acttcaggcg atgatagcat
   136501 aataattgcc catggaggca agggaggtaa aggaaatagc catttcaagt catctaccaa
   136561 tcgcacgccc agaaaggcag agccgggaca aaaaggtgag agaaaaaaac tatctttgga
   136621 gctcaagctt ttggcagacg taggaatagt aggtcctccc aactccggca aatcaaccct
   136681 tttggctaaa gtttctcagg ctcgaccgaa aatagcggat tatcctttta ctaccctgtc
   136741 ccccaatttg gggattgtta gaatatcaga tagtaagagt ttcgtaatgg ctgatatccc
   136801 cggcttaatt gagggagcgc atcaaggaaa aggattggga tttgattttc tcaaacatat
   136861 tcagagaact agaattttgc tttatctaat tgatattacc tcaccggaca tatccggaga
   136921 gtttgtcaag attagaaaag agcttaaact ttttgacaaa aaactttttg accggccttc
   136981 ggttttggcc ttgaacaaga tcgatctgct cttcccggaa caaagaaaaa agataaagat
   137041 ggcaaagcgc ggaattccag ttttcaagat ttcagcagct aatggggaag gggtgaagaa
   137101 attggcgaac tttctttctt taaaactgac tcttcttgtg gaaggggaaa attgagccag
   137161 gacgttctct cgtatttagc cttatactcc gtgccggagg tagggactca aaggataatc
   137221 tctctagtaa ggaaattcgg ctctgccaaa gaggcgctag gtgcttctct aaaagagctg
   137281 accagagtgg aagacatagg agataaaatt gcccataata tcaaacacca agtcaactgg
   137341 aagttagcac aagaacaatt tagtttagct gaaaagaaga aattcaagat tttgactttc
   137401 tttgacgaag aatatcctaa aagtttgaaa aatatatacg acccaccacc tttgattttt
   137461 ttcagggggg aaataagatc ggaagatcaa aaagccatag cagtagttgg ctcaagaaaa
   137521 gcgagctctt atggaagaat ggttaccgag agtctggtgg aggaattagc ctctcgcggc
   137581 ataaccatag tcagtggctt agctcgaggc atagattcaa ttagccacca ggccgcgtta
   137641 aaagcaggtg gaaggacact tgcggtttta ggaagcggtc tggacataat ctatcctccg
   137701 gaaaatagaa aattggcaga cgggatagcg gaaaacgggg cggtcatatc ggagttcttc
   137761 ttagggacga agccggaggc aaccaatttc ccccagagga atagggtgat aagtggactt
   137821 tgcttaggca tagtgattgt cgaagctggt cccaaaagcg gagcgctctt gacagcccgg
   137881 cacgccttgg agcagaatcg ggaagtattt gctgttccgg gagatatacg ttctcagggg
   137941 agcaaaggga ctaatgagtt gatcaaacaa ggagcgaaat tagttagttc agtggaagat
   138001 atcttagttg agctgaatga attgactaat gcttcagcta aaaaagaaag aatagagaga
   138061 gtggagcagg acctgcccaa agaggaaaaa gacatattca agctcttgtc cattgagcca
   138121 tatcacatcg acaaaatctc caaagaatta gggctgcgta gctccgaggc gctctctttt
   138181 cttctttcct tggagctcaa ggggttagtc aaacagttgc ccggaaaagt gtttgcccgg
   138241 gcttgaatag ggagaaaaat tgatagagag aaacgtggaa ataatcaata aattgggatt
   138301 acacgcccgg ccagctgcta tcttcgtgca aaaagccagc aagtttaagt ctgagatcac
   138361 ccttgaaaaa gaagggcaaa aggtaaatgg gaagagcata atgggggtga tgatgttggc
   138421 ggcggagaag gggagcactc ttatcataag agccgaagga gaagatgaga atgaggcggt
   138481 ggcggctttg gttgaggttg tcttgagtaa atttggagag gagtgacctt ggaaaagcag
   138541 gcacaaaaaa atatcctctt aaaaggagtg tccgcatctc cgggaattgt aatcggtgag
   138601 actttcctct ttgatagaaa gatagttgaa gccaaggagc aagagctgaa ggaagaggag
   138661 ataggcaagg agcttctcaa gttcaagaag gctctggctg agactaaaag ggaaatgatc
   138721 aatctaaggg ataaggtgga gcggcgaatc gatccggacc acgctaagat atttgatgcg
   138781 cagattatga tcttggagga ccaatggata aatcaaaa
//
LOCUS       DASWLN010000169         2903 bp    DNA     linear   ENV 20-FEB-2024
DEFINITION  MAG TPA_asm: Candidatus Zixiibacteriota bacterium isolate
            SMAG_U2949 k141_222718, whole genome shotgun sequence.
ACCESSION   DASWLN010000169 DASWLN010000000
VERSION     DASWLN010000169.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37960594
            Sequence Read Archive: SRR10376091
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Candidatus Zixiibacteriota bacterium (soil metagenome)
  ORGANISM  Candidatus Zixiibacteriota bacterium
            Bacteria.
REFERENCE   1  (bases 1 to 2903)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 2903)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (26-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/12/2024 08:28:26
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 1,440
            CDSs (total)                      :: 1,416
            Genes (coding)                    :: 1,412
            CDSs (with protein)               :: 1,412
            Genes (RNA)                       :: 24
            rRNAs                             :: 1 (16S)
            partial rRNAs                     :: 1 (16S)
            tRNAs                             :: 21
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 4
            CDSs (without protein)            :: 4
            Pseudo Genes (ambiguous residues) :: 0 of 4
            Pseudo Genes (frameshifted)       :: 0 of 4
            Pseudo Genes (incomplete)         :: 3 of 4
            Pseudo Genes (internal stop)      :: 1 of 4
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2903
                     /organism="Candidatus Zixiibacteriota bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_222718"
                     /isolate="SMAG_U2949"
                     /isolation_source="soil"
                     /db_xref="taxon:2053527"
                     /environmental_sample
                     /country="USA: Oregon"
                     /lat_lon="42.99 N 123.63 W"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            complement(<1..248)
                     /locus_tag="VGB16_06260"
     CDS             complement(<1..248)
                     /locus_tag="VGB16_06260"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003518029.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEX9917324.1"
                     /translation="MPMEKKGWWLPSLFVQMRKVCFFTIGCKLNQYETQAISEGLEKL
                     GFQRVNFGEKADLYLINTCTVTAQSDRSARQTIYRAKR"
     gene            complement(259..2286)
                     /gene="rpsA"
                     /locus_tag="VGB16_06265"
     CDS             complement(259..2286)
                     /gene="rpsA"
                     /locus_tag="VGB16_06265"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011358458.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003676 - nucleic acid binding [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="30S ribosomal protein S1"
                     /protein_id="HEX9917325.1"
                     /translation="MAQEKTKKLKGKDERKGGEKKTKSVGKLKKAPKKKVSASKEKGL
                     KGKVALEVKAVELEKKPIRKRATKATKEAGKEKPEQKPTLILPMKELEREEGYTPEEY
                     EEMAKMYDSTMHDIREGELVTGKVIAVTKEDVIVDVGFKSEGVIPIYEFGDTPNVQVG
                     EQVEVFLEAVEDQDGQLVLSKQKADFMRVWDRVKDAYEKGALVEGKLVRRIKGGIVVD
                     VYGVEAFLPGSQIALRQVQDFNALINRKMELKVIKVNKMRRNIVVSRRSVLEEEREKL
                     RAKLLGEIETGQVWEGLVKNITDFGVFMDLGGVDGLLHITDMSWGRISHPSELVALGD
                     KIKVKILDFDKKTGRVSLGLKQLTPYPWENIEQKYPVGSKVKGKVVSVTDYGAFVELE
                     RGVEGLIHISEMSWTQHIKHPSKLVGVSDIVEVMVLSVDKENEKVSLGLKQLEPDPWQ
                     SLEQKYPVGSKAGGKVRNLTAFGAFVELEEGIDGLIHISDMSWTKRIQHPGEVLKKGD
                     RAEVVILSIDKENRKVSLGYKQLKPDPWPELAKKFIIGTQTRGKISRLLDRGVIVELE
                     GEVEGFVPTSQLGKEDLSKPADAFAEKEELPLRVIEFDQGEKRIVLSVNAYYEGKERA
                     EFEEYLTKHPIKTVKLEDVVTEIPKVEPPITQEQKPIDQSPPAEGEQGTAG"
     gene            complement(2338..>2903)
                     /gene="ispH"
                     /locus_tag="VGB16_06270"
     CDS             complement(2338..>2903)
                     /gene="ispH"
                     /locus_tag="VGB16_06270"
                     /EC_number="1.17.7.4"
                     /inference="COORDINATES: protein motif:HMM:TIGR00216.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0051745 - 4-hydroxy-3-methylbut-2-en-1-yl
                     diphosphate reductase activity [Evidence IEA];
                     GO_process: GO:0009240 - isopentenyl diphosphate
                     biosynthetic process [Evidence IEA];
                     GO_process: GO:0015968 - stringent response [Evidence
                     IEA];
                     GO_process: GO:0046677 - response to antibiotic [Evidence
                     IEA]"
                     /codon_start=3
                     /transl_table=11
                     /product="4-hydroxy-3-methylbut-2-enyl diphosphate
                     reductase"
                     /protein_id="HEX9917326.1"
                     /translation="ATCPFVKAAQEKAALLKRQGYQVVVIGEKNHPEVQGIVGYAGKG
                     TIVIDQNEHFPNLSRFKKLGIVTQTTYNFDAFSKSVSKLLNSAKEIKIFNTICNATAD
                     AQKNALDLAKKVNLMIVVGGYNSANTSRLTQLCRGASRKTYHIETANQLKKKWFKGVK
                     KVGVTAGTSTPDWIIREVVSKIKEMGN"
BASE COUNT          652 a          839 c          511 g          901 t
ORIGIN      
        1 aatctcttgg ctctatatat ggtttggcga gcagagcggt cactctgggc agttactgtg
       61 caggtattga taaggtataa gtcggctttc tctccaaaat tgaccctctg aaatccgagc
      121 ttttctaatc cctctgatat agcctgggtc tcatattgat tgagcttaca cccaatagtg
      181 aaaaaacaaa cttttctcat ctgaacaaaa agggatggca accaccatcc ctttttctcc
      241 attggcataa aaatctacct aaccagccgt cccctgttcc ccttcagccg gaggcgactg
      301 atctattggc ttctgctcct gagttatggg tggttcaacc ttggggattt cggtcaccac
      361 atcctcgagt ttcaccgttt taatgggatg ttttgtcagg tattcctcaa actcagccct
      421 ttcttttcct tcgtaatagg cgttcaccga aaggacgatc ctcttctcac cttggtcaaa
      481 ctctatcacc ctcaaaggca actcttcctt ctctgcaaag gcatccgccg gcttggaaag
      541 gtcctccttg cctaattgac tggtaggaac aaaaccttca acttcacctt ccagttcaac
      601 tatcactcct cgatccagaa gacgcgatat cttacctctg gtctgggttc ctatgatgaa
      661 tttctttgcc agctctggcc aaggatcagg tttcagctgc ttatatccca atgaaacctt
      721 gcgattctcc ttatcaatgc tgaggatgac cacctctgcc cggtctccct ttttcaggac
      781 ttcgcccggg tgctgaattc ttttggtcca ggacatatcg gaaatatgaa tcaggccgtc
      841 tatcccctct tccagctcca caaacgcacc gaaagcggtc aggttcctga cttttccacc
      901 agctttcgag ccaaccgggt atttttgctc taaactctgc caggggtcgg gctccaattg
      961 ctttaagccc agggatacct tctcattctc tttatccacg gagagcacca tcacctccac
     1021 gatatcactc actcccacca atttggaagg atgtttgatg tgctgtgtcc aagacatctc
     1081 tgagatatga attaaccctt ctactcccct ttccaactca acgaaagcgc cataatcggt
     1141 cacagaaact accttccctt taaccttgct gccaactggg tatttctgct cgatattctc
     1201 ccaaggataa ggagttagct gttttaagcc caaggagacc cggccggtct tcttgtcgaa
     1261 gtccaatatt ttgactttta tcttatcccc taaggctacc aattcgctcg gatgactaat
     1321 tcttccccag gacatatccg ttatgtgcag tagtccgtct actcctccca ggtccatgaa
     1381 aacgccgaaa tcggtgatgt ttttcaccag tccttcccac acctgaccgg tctcaatttc
     1441 acctaagagt ttagctctca gtttttctct ctcctcctcc agaactgacc gacgtgagac
     1501 cacgatattg cgtctcatct tattgacctt tatgaccttc agctccattt tcctattaat
     1561 caaggcattg aagtcctgaa cttgacgtaa ggcaatttgc gagccgggca gaaaagcctc
     1621 cactccataa acgtccacca ctattccacc ttttattcgg cgaacgagct tcccctccac
     1681 cagagctccc ttttcataag catctttaac tctatcccaa acccgcatga aatctgcttt
     1741 ctgcttggaa agaaccagct gcccatcctg gtcctcaacc gcctccaaga aaacttcgac
     1801 ctgctcccca acctgcacat tcggggtatc gccgaattcg taaatgggaa taaccccttc
     1861 ggacttaaaa ccaacatcca ctatcacatc ctctttggtc actgcaatca ctttgccggt
     1921 aaccagctct ccctcccgta tgtcatgcat agtggaatca tacatcttag ccatctcttc
     1981 atattcctca ggagtatatc cctcctccct ctccaattcc ttcattggga gaatcaacgt
     2041 gggcttctgc tctggctttt cttttcctgc ctctttggta gctttggttg ccctttttct
     2101 aatcggcttc ttttcgagct ccaccgcttt tacctctaaa gcaactttcc cttttagtcc
     2161 tttctcctta cttgcggaaa ccttcttttt gggggctttc ttcagcttcc caaccgactt
     2221 ggtcttcttt tcaccccctt tcctttcatc tttacctttc aacttcttgg tcttctcttg
     2281 cgccattgct tgtaacgcct cctttatatt gaatttgccc cgaagggcat gaaagttcta
     2341 atttcccatc tccttgattt tactaactac ctctcggatg atccagtccg gagtggaagt
     2401 gccagctgtc actcccacct tctttacccc cttaaaccac tttttcttta actggttcgc
     2461 agtctctata tggtaagttt ttctgcttgc tcctctgcac aattgcgtca gacgcgaggt
     2521 attggctgaa ttatatccac ccaccactat catcaagttc acttttttag ctaaatccaa
     2581 agcattcttt tgggcatcag ctgtggcgtt gcagatggta ttgaatattt ttatctcctt
     2641 agcggagttt agtaatttag ataccgattt ggagaaagca tcaaaattgt aggtagtttg
     2701 tgtcactatc cctaacttct tgaacctgga tagattaggg aaatgttcat tttgatctat
     2761 gactatagtc cccttcccgg cataccccac aatcccctgc acctctggat gatttttctc
     2821 accaatcacc accacctgat aaccctgcct tttaagcaag gctgctttct cctgggcagc
     2881 tttaacaaaa gggcaagtgg cat
//
LOCUS       DASWLN010000170        10095 bp    DNA     linear   ENV 20-FEB-2024
DEFINITION  MAG TPA_asm: Candidatus Zixiibacteriota bacterium isolate
            SMAG_U2949 k141_1113671, whole genome shotgun sequence.
ACCESSION   DASWLN010000170 DASWLN010000000
VERSION     DASWLN010000170.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37960594
            Sequence Read Archive: SRR10376091
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Candidatus Zixiibacteriota bacterium (soil metagenome)
  ORGANISM  Candidatus Zixiibacteriota bacterium
            Bacteria.
REFERENCE   1  (bases 1 to 10095)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 10095)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (26-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/12/2024 08:28:26
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 1,440
            CDSs (total)                      :: 1,416
            Genes (coding)                    :: 1,412
            CDSs (with protein)               :: 1,412
            Genes (RNA)                       :: 24
            rRNAs                             :: 1 (16S)
            partial rRNAs                     :: 1 (16S)
            tRNAs                             :: 21
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 4
            CDSs (without protein)            :: 4
            Pseudo Genes (ambiguous residues) :: 0 of 4
            Pseudo Genes (frameshifted)       :: 0 of 4
            Pseudo Genes (incomplete)         :: 3 of 4
            Pseudo Genes (internal stop)      :: 1 of 4
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..10095
                     /organism="Candidatus Zixiibacteriota bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_1113671"
                     /isolate="SMAG_U2949"
                     /isolation_source="soil"
                     /db_xref="taxon:2053527"
                     /environmental_sample
                     /country="USA: Oregon"
                     /lat_lon="42.99 N 123.63 W"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            complement(<1..732)
                     /gene="recA"
                     /locus_tag="VGB16_06275"
     CDS             complement(<1..732)
                     /gene="recA"
                     /locus_tag="VGB16_06275"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_006927867.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0005737 - cytoplasm [Evidence IEA];
                     GO_function: GO:0003677 - DNA binding [Evidence IEA];
                     GO_function: GO:0008094 - ATP-dependent activity, acting
                     on DNA [Evidence IEA];
                     GO_process: GO:0006281 - DNA repair [Evidence IEA];
                     GO_process: GO:0006310 - DNA recombination [Evidence IEA];
                     GO_process: GO:0009432 - SOS response [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="recombinase RecA"
                     /protein_id="HEX9917327.1"
                     /translation="MDKDIIQGKNKALEQAVSQIEKQFGKGSIMKLGDKPISAEIGII
                     PTGSIGLDAALGIGGIPRGRVIEIFGPEASGKTTLALHIIAEAQKRGGTAAFIDAEHA
                     LDSAYAKNLGVDIDNLMISQPDTGEQGLEITETLVRSGAVDTIVIDSVAALVPRAEIE
                     GEMGDSHMGLQARLMSQALRKLTGTISKSRTSVIFVNQTRMRIGIMFGNPETTTGGMA
                     LKFYSSVRIDIRRISSLKEGQNVVGS"
     gene            complement(746..1300)
                     /gene="thpR"
                     /locus_tag="VGB16_06280"
     CDS             complement(746..1300)
                     /gene="thpR"
                     /locus_tag="VGB16_06280"
                     /inference="COORDINATES: protein motif:HMM:TIGR02258.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008664 - 2'-5'-RNA ligase activity
                     [Evidence IEA];
                     GO_process: GO:0008033 - tRNA processing [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="RNA 2',3'-cyclic phosphodiesterase"
                     /protein_id="HEX9917328.1"
                     /translation="MRTFIAIELPSDTKEKIEEAISPFRKLNLSVSWVKSKNLHLTLK
                     FLGEVDEKKVDDIVLALEQAVKGKEKFEMDLKDFGTFPNLKRPRVIWIGIEKGKESLI
                     VIQNEIEKELSKLGFPQEEKGFSPHLTIGRVKSAEGIERLTEQIKNVNFVVEDIEVGE
                     VVLMRSQLHPQGAAYTPIRKFKLK"
     gene            complement(1301..2536)
                     /locus_tag="VGB16_06285"
     CDS             complement(1301..2536)
                     /locus_tag="VGB16_06285"
                     /inference="COORDINATES: protein motif:HMM:NF001813.0"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="competence/damage-inducible protein A"
                     /protein_id="HEX9917329.1"
                     /translation="MRAEIITIGDEILYGQTVDTNATFIGQRLTQLGIEVALRTSVGD
                     DIEKIGQAINNARSRADLIITTGGLGPTDDDLTKKAIVKVFKRNLIFHEEILKKVEEG
                     FVKRGMKMPKINQNQALIPQGSKTIDNHWGSAPGIFIQEDKLLFFALPGVPLEMKTML
                     DEGILPILRETIPQITTLHRTLRTTGIAESAIYEKIKRIVKSKSPIKFAFLPSFLGVD
                     IRLTITSPNIKEAQEKIAERERKLREILDEYIYGTDDQILEGVVGELLLKKKMALATA
                     ESCTGGLLGVKITNISGSSKYFERGVITYSNQAKTDLLGVPKQLIEKYGAVSQEVAVS
                     MVEGVRKLAGVDIGISITGIAGPAGGTAEKPVGLVYIGLASKDSSFAEKFQFGEERET
                     NRQRAAQAALNMLRLYLVK"
     gene            complement(2552..3013)
                     /locus_tag="VGB16_06290"
     CDS             complement(2552..3013)
                     /locus_tag="VGB16_06290"
                     /inference="COORDINATES: protein motif:HMM:NF016489.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="phosphatidylglycerophosphatase A"
                     /protein_id="HEX9917330.1"
                     /translation="MPKSIIKILATFFSVGYFPIAPGTLASLVTLVLAWFLLPQETII
                     NLFVCLAIILVAVWSGTKAEEFWGEDNRRIVIDEVAGMLVSIFLVSRSIRLYLAAFLL
                     FRFFDVLKPFPIRKLEKLKSGWGVTTDDIAAGVYANITLLLLLYLRNILKT"
     gene            complement(3015..3602)
                     /gene="pgsA"
                     /locus_tag="VGB16_06295"
     CDS             complement(3015..3602)
                     /gene="pgsA"
                     /locus_tag="VGB16_06295"
                     /EC_number="2.7.8.5"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011099516.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008444 -
                     CDP-diacylglycerol-glycerol-3-phosphate
                     3-phosphatidyltransferase activity [Evidence IEA];
                     GO_process: GO:0008654 - phospholipid biosynthetic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="CDP-diacylglycerol--glycerol-3-phosphate
                     3-phosphatidyltransferase"
                     /protein_id="HEX9917331.1"
                     /translation="MNFPNKLTIARIFLAPIFMVFFLIKDVYLGYLATLVFCLAAATD
                     LYDGYLARKTGVITGFGKFMDPLADKILTSVALISFVALGYVETWIVSVIIVREFLVT
                     GLRSLAAYKGLVIMPSIPAQWKTALQMITIIIILLFINLKSTLVPLGYHWRIFDQALS
                     IRLFNIMVFVLMLMSVGTGIDYLVKNAPLLKGVLK"
     gene            4188..6308
                     /gene="recG"
                     /locus_tag="VGB16_06300"
     CDS             4188..6308
                     /gene="recG"
                     /locus_tag="VGB16_06300"
                     /EC_number="3.6.4.12"
                     /inference="COORDINATES: protein motif:HMM:NF008168.0"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003676 - nucleic acid binding [Evidence
                     IEA];
                     GO_function: GO:0003678 - DNA helicase activity [Evidence
                     IEA];
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_process: GO:0006281 - DNA repair [Evidence IEA];
                     GO_process: GO:0006310 - DNA recombination [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="ATP-dependent DNA helicase RecG"
                     /protein_id="HEX9917332.1"
                     /translation="MQTTLKSIKKSIPLKLDSPAQYLKGIGPKRAKVLASQEIRTVED
                     LLYYIPRKYLDRSTITPIDQLKANSVATVVGRVVGYETKKGRIARFQAVIEDKTGSLT
                     LVWFRGMNYITNMFEPGDVIIASGEVRFYYGLQMAHPEFERISEGDEELIHTGRVIPV
                     YPSSADFKHFGLDSRGLRRILNPLLSKTESLISEKLPDQIITELGLMPLPETIRNIHF
                     PDNLEMASKARQRLAFDEFFFFELMMALRKKKLNQKQGGIVFKPSDSLVRKLLDRLSF
                     ELTPSQEKVLREISEDMTSTKVMNRLLQGDVGSGKTIVALIAMLIAVESGYQAALMAP
                     TEILAEQHFINIRSLLEKLGLGVVFLTSSVKKNQKENILQKIENGTVQIIVGTHALIQ
                     ESVVFHKLGLVVIDEQHRFGVVQRAKLRKKGILPDVLVMTATPIPRTLALTLYGDLDV
                     SVIDQMPPGRKSIRTVWWKKSSPEELYKFLMEEINKGRQAYIVYPLVEESEKADLKAA
                     TESFHLLQEKIFPQLKLALIHGRIKSEEREKIMRDFRCGKYDILVTTTVIEVGLDVSN
                     ATLMLIEHADRFGLSQLHQLRGRIGRGEEQSYCFLLTEGNSSMEAHQRLNTMVSTTDG
                     FKIAEVDLKLRGPGEFFGTRQHGLPDFKIADLVGDMDLLYQARKQAFKLVEEDPYLRS
                     ERNWMVKNNLTAKYKNKFSLIEVG"
     gene            6330..6887
                     /locus_tag="VGB16_06305"
     CDS             6330..6887
                     /locus_tag="VGB16_06305"
                     /inference="COORDINATES: protein motif:HMM:NF015950.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF366 family protein"
                     /protein_id="HEX9917333.1"
                     /translation="MKFLFIQEKIKFTGEQLLSQWAYKNHDLLGDSFVAFIGPCEIPE
                     KYMVALDHLKKKSTIYSENMLHFVIEHFDLDLEKAILRQKLLLAILKDKLNHRLGGDV
                     LQRWGDDLYDGDAKLTVSTTVRTPVSTKIHMGINISSKNVPVKAKGLEDYSIDPFDIA
                     QVVMNQYILDMRRISERIAKSRYVE"
     gene            6917..7111
                     /locus_tag="VGB16_06310"
     CDS             6917..7111
                     /locus_tag="VGB16_06310"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEX9917334.1"
                     /translation="MQDGKKLDKIILDVEKCEISIKKFIEQVQDYDMERILKKVDAEL
                     MDALHNLNLAKKQMQPKKAE"
     gene            7159..8532
                     /locus_tag="VGB16_06315"
     CDS             7159..8532
                     /locus_tag="VGB16_06315"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_014221279.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016787 - hydrolase activity [Evidence
                     IEA];
                     GO_process: GO:0008152 - metabolic process [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="HAD-IB family phosphatase"
                     /protein_id="HEX9917335.1"
                     /translation="MLSVVVPVLNEERTIGKVVRFAKQAQSVDEVVVVDDKSIDNTVE
                     EAKNAGAKVVISTKIGKGASMRDGLLVSKNEIIVFLDGDIENYAPDTIEKMVFPIIGG
                     EADFVKATFEREAGRVTELVAKPLLNLLFPQLSHFSQPLSGMIAGRREHFEKITFDNG
                     YGVDIGILIDMHLLGAKIVEVNIGAIKHKMKLWRELGRMSREVAEAILKKAVNNPNIS
                     LDSLGTINIIRDQMEFALKETLSTLKKMVIFDMDNTVLDGRFIDKVALTFGFQEQLLD
                     IITKNQEPYLRTKLIAKLFKGLNISQLLSVTDDIPIVSDTIEVVKELKKRSYIVGIIS
                     DSYDCIAAHIKNKVGADFSLANELEFSQGIATGEVKIPSFFMKQDKSICNHSFCKSNA
                     LFQVAEQYGIELSNIIAVGDSESDICVVKYAGIGVAFCSSNSILNAIADQRIETRSFK
                     QILDFAI"
     gene            9024..9347
                     /locus_tag="VGB16_06320"
     CDS             9024..9347
                     /locus_tag="VGB16_06320"
                     /inference="COORDINATES: protein motif:HMM:NF020473.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF1844 domain-containing protein"
                     /protein_id="HEX9917336.1"
                     /translation="MEQEKKISEDEALFVQLVLMFQAAAMQQLGKLINPLNGKTERNL
                     EQAKFSIDILDMLKSKTEGNLSEEEKRLLEHVVYELQMNYLDELKNKEKQPEKDTPKD
                     KPTSN"
     gene            9406..>10095
                     /locus_tag="VGB16_06325"
     CDS             9406..>10095
                     /locus_tag="VGB16_06325"
                     /inference="COORDINATES: protein motif:HMM:NF013714.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016491 - oxidoreductase activity
                     [Evidence IEA];
                     GO_function: GO:0050660 - flavin adenine dinucleotide
                     binding [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="FAD-binding protein"
                     /protein_id="HEX9917337.1"
                     /translation="MKEHKKYRDFVGLTGPEVRFGEDLANWTTLGIGGRCDLFYAAKK
                     PEDLIRVVLASKRFSIPYFVLGGGSNLLVSDKEFRGLVIKNKCNRIRVEKNRIICQSG
                     ALLKDIVSIAKTHSLAGLEFAVGIWGTVGGAVYGNAGAFAQSIGDILEQGVVLTSSGR
                     IENVKKDFFEFGYRDSKLKSSRDILLSVVFKLKKGNSKSIEDKMRKNLAQRSIRVPNR
                     MKTAGCYFKNPQ"
BASE COUNT         2979 a         2105 c         2177 g         2834 t
ORIGIN      
        1 agagccaacc acattttgcc cctctttgag gctggatatt cttcttatat ctattctgac
       61 agaagagtag aatttcagag ccatcccccc agtggtggtt tccgggttgc cgaacattat
      121 tccaattctc attcgggtct ggttaacgaa aataacgctg gtcctggatt tgcttatagt
      181 tccggtcagt ttccttaatg cctgcgacat taatctggct tgcaagccca tatgtgaatc
      241 acccatctca ccctcgatct ccgccctggg aaccaaagcc gcaactgaat caatcacgat
      301 ggtatccacc gctccacttc tcaccagagt ctccgttatc tccaaacctt gctctccagt
      361 gtccggctgg gaaatcataa gattatcaat atccacgcct aaatttttgg cataagccga
      421 atccagggca tgctccgcat ctatgaaagc agcagtacca cctctttttt gggcttcagc
      481 aatgatatgc aaagccaggg tagttttacc agaagcttct ggcccaaata tttcaatcac
      541 tctaccgcgg ggtattccac ctattcctag agccgcatcc agaccgattg agccggtggg
      601 gataatgccg atctcggcac taattggctt atcccccaac ttcataatcg agcccttgcc
      661 aaactgcttt tctatctggc ttaccgcctg ctccaatgct ttgttctttc cctgaatgat
      721 atccttatcc attttccctc accttctatt ttaatttgaa ttttcttata ggggtataag
      781 ccgcaccctg cggatgaagc tggcttctca ttaaaactac ctcacccacc tctatatcct
      841 caaccacaaa gttaacattt tttatctgtt ctgtcaatct ctcaattcct tcagcggatt
      901 taacccgacc tatggtcaga tgtggcgaaa accctttttc ttcttgagga aagcccaatt
      961 tggaaagctc cttctcaatc tcattttgta tcactatcaa gctctctttg cccttttcta
     1021 tacctatcca gataactctg ggtcttttaa gattagggaa agtcccaaaa tctttcaaat
     1081 ccatttcaaa tttttctttc ccttttaccg cttgctctaa agccaaaact atatcatcta
     1141 cttttttctc atccacttcg cctaaaaact tcaaggtcaa gtgaaggttc ttagacttaa
     1201 cccaggacac agataaattc agctttctaa aaggggagat agcttcttct atcttttcct
     1261 tggtatctga aggaagttca atggcaatga aggttctcat ctattttact agatacagtc
     1321 ttaacatatt caaggcagct tgagcagccc tttgacgatt agtttccctc tcctccccga
     1381 actggaattt ttccgcaaat gaactgtcct tggaagcaag cccaatataa accaagccca
     1441 ccggcttttc agcggttccg cccgcaggtc cggcaattcc ggtaatggat attccaatat
     1501 caactcctgc caattttctc actccctcta ccatagaaac tgccacctcc tgactaaccg
     1561 caccatactt ttctattaat tgcttgggga ctccaagtaa gtccgttttc gcctgattgc
     1621 tgtaagttat aactcccctt tcaaaatatt tcgagcttcc ggagatatta gtgattttga
     1681 ctcccaataa gccaccagtg caggactcgg ctgtggcaag agccattttc tttttcaaaa
     1741 gcagctctcc caccacccct tccaaaatct ggtcatctgt cccatatatg tattcatcca
     1801 aaatctccct tagttttctt tctctttccg ctattttctc ctgagcctct tttatattcg
     1861 gggaagtgat ggtcaatcta atatccaccc ccaaaaaact cggcaagaaa gcaaacttta
     1921 ttgggctttt tgatttaaca atccttttta ttttctcgta gatggcggat tcagctatgc
     1981 cggtggttct gagagttcta tgcagagtag taatctgtgg aatcgtctcc cttaagattg
     2041 gcagtattcc ctcatccaac atagtcttca tctccaaggg aactccaggc aaggcaaaaa
     2101 acaacaattt atcctcctga atgaatatgc ccggggctga accccaatgg ttatctatag
     2161 tcttgcttcc ctgtggaatc agtgcctggt tttgatttat cttgggcatc ttcattccac
     2221 gcttaacaaa accctcctcc actttcttga gtatctcttc gtgaaatatc agatttctct
     2281 taaaaacctt gacaatggct tttttagtca aatcatcatc tgttgggccc aagccaccag
     2341 tggttattat caggtccgcc cgggaccggg cattatttat ggcctggcct atcttttcaa
     2401 tatcatcgcc tacgctggtg cgtaaggcta cctcaattcc tagttgagtc aacctttgtc
     2461 cgataaaggt ggcgttggta tcaactgttt gaccatatag aatttcgtct ccgatagtta
     2521 tgatttctgc cctcattttc ttaaattctc cttaagtttt taaaatgttc cttaaataaa
     2581 gtaaaagaag tagagttatg ttagcgtata ccccagctgc tatatcatct gtggttactc
     2641 cccagccgga ttttaacttc tccaattttc ttataggaaa gggtttaagc acatcaaaaa
     2701 atctgaacaa aaggaatgcg gctaaataca atctgatgct tctgctcacc agaaatatcg
     2761 aaaccagcat cccagccacc tcatcgatta ctattctcct attgtcctct ccccaaaact
     2821 cttctgcctt agtgcctgac catacagcca caaggataat ggctaaacag acaaatagat
     2881 ttattatagt ttcttgtggc aacaagaacc aggcaagaac caatgtcact aaactggcta
     2941 aggttcccgg agctattgga aaatatccaa ccgagaagaa agtggctaag attttgatta
     3001 tagatttggg caatctattt caatactccc tttaaaaggg gggcgttctt gactaaataa
     3061 tcaattccgg tgccaacgct cataagcata agcacaaaga ccatgatatt aaataacctg
     3121 atacttaggg cttggtcaaa tatcctccaa tggtatccca gagggaccag ggtagatttc
     3181 agattaataa acaataaaat gattatgata gttatcatct gcaaggcggt tttccactga
     3241 gccgggatgc tgggcattat caccaatccc ttataagcag ccaaacttct caaaccggtt
     3301 accaaaaact ccctcactat gattaccgaa actatccaag tctcgacata acccaacgct
     3361 acaaaagaga taagggcaac cgaggtgagt atcttatctg ccagcggatc cataaatttg
     3421 ccgaatccgg tgatgacccc ggtctttctg gctaaatatc cgtcatacag gtcagttgcc
     3481 gctgccaaac aaaagaccag cgtggcaaga taccctaaat agacatcctt gattaagaag
     3541 aagaccatga aaatgggagc aagaaatatg cgagctatgg tcagtttgtt agggaagttc
     3601 atccgggaat gaaatttaaa ctaattgttc tggtaaatct aatattcctt gtcagtcaag
     3661 tcaacacatt tctccgaatc aaatcttcaa tatcagaccc gagtgagccc aaagagacgg
     3721 ctgaaaaagt ccagcaaagt ggttgggtct caagatgcag aattcacgca gggtgcctgg
     3781 cctacgaaaa atcaatgact tatctcgaga tacagaactc gtgcaagaat ccattttgtt
     3841 tttataacta aaagccatca agctggattc ctcccgcctt tggcgggacg ggaatgacaa
     3901 acaaacgcga tagtttcata gatacccacg cctttatagg cgtggttctt gttgtttgta
     3961 ggggcaattc atgaattgcc cctactacgc tttgtcatcc ccgagggttt aatcggggat
     4021 ccatacttac ttcttctctt ctggattcct gctggagttt atccctgcgg aagcaggggc
     4081 aggaatgaca aacagacata caccagcctt cttttttaaa aagataagct tccctaaaat
     4141 caaaagtcct tgacagttga ttaaaagagc tttttattag aaagcggatg caaaccacac
     4201 tcaaatctat aaaaaagtcg attcctctga aactggatag cccggcccaa tatcttaaag
     4261 gcatagggcc caaaagagca aaggtcttgg cttcacaaga aattaggaca gtagaggatt
     4321 tgctttacta tattccccga aaatatcttg accgcagcac cattacccca attgaccaac
     4381 ttaaggcgaa ctctgttgcc acagtggtag ggagggtagt tgggtacgag acgaagaaag
     4441 ggagaattgc tcgattccag gcggtaatag aggataagac cggcagtctt actttggtct
     4501 ggttcagagg gatgaactat attacaaata tgtttgagcc gggagatgtg ataatcgctt
     4561 caggtgaagt taggttctat tatggtttac aaatggcgca tccggaattc gaacggatat
     4621 ctgaaggaga cgaggagctg attcatactg gcagagttat tccggtttat ccttcgagtg
     4681 ccgatttcaa gcacttcggt ttggatagcc ggggcttaag gaggatatta aacccactct
     4741 tgagcaaaac cgaatcccta atatccgaga aattacctga ccagatcata actgagcttg
     4801 ggcttatgcc gttgccggaa actataagaa atatccattt tccagacaac ttggagatgg
     4861 caagtaaagc ccggcagagg ttagcttttg atgaattttt cttttttgag cttatgatgg
     4921 cattgcgtaa gaaaaagcta aatcaaaaac agggtgggat tgtttttaaa ccttcagaca
     4981 gcttagtgag aaaactttta gatcgactct cctttgaact taccccttca caggagaaag
     5041 tcctgcgtga gatatcagaa gatatgacct ccaccaaggt catgaatcgc ctattgcagg
     5101 gagatgtcgg ctcaggcaag accatagtag ctttgatagc tatgcttatt gcagtggaaa
     5161 gcggttatca ggctgctctc atggctccta ctgaaatctt agcagaacaa cattttataa
     5221 acatccgttc tctattagag aagctcggtt tgggggttgt gtttttgacc agttcagtta
     5281 agaaaaatca aaaagagaac atacttcaaa aaatagaaaa cggaacagtg cagattattg
     5341 tgggcactca tgctttgatt caggaatcag tcgttttcca taagttaggg ctggtggtga
     5401 ttgacgaaca gcatcgcttt ggagtagtgc agagagcaaa acttagaaaa aagggaatcc
     5461 tgcctgatgt tttggttatg accgctaccc cgatcccccg aactttagcc ctaaccttgt
     5521 atggcgattt agatgtctca gttatagacc agatgccacc tggaagaaaa agcatccgca
     5581 ctgtttggtg gaagaaaagt tcaccggagg aattatataa gtttctgatg gaggagataa
     5641 ataagggcag gcaggcttat atcgtttatc ctttagtgga ggaatctgaa aaggcggacc
     5701 tgaaagccgc cactgaaagt tttcatcttt tgcaggagaa gatattccct cagctgaaat
     5761 tggccttaat tcatggccgc ataaaaagcg aggagaggga gaaaataatg cgtgattttc
     5821 gttgcggaaa gtacgatatt ctggttacca ccacagtcat cgaggtgggg ttggatgtgt
     5881 ccaatgctac cttaatgtta atcgaacatg cagacagatt tggcttgtcc cagttgcacc
     5941 agctgagggg aagaattggt cggggagaag agcaatctta ttgtttcctt ctaactgagg
     6001 gaaattcgtc catggaagca caccaaagac tgaatactat ggtcagcacc acggacggtt
     6061 ttaaaatagc ggaagtggat ttgaaactaa gaggtccggg agaattcttc ggcacccgac
     6121 aacatggtct gccggatttc aagatagcgg acttggtggg tgatatggac cttttatatc
     6181 aggcccgcaa gcaagctttc aagttagttg aagaagaccc atatcttaga agtgaaagga
     6241 attggatggt caaaaataac ctgaccgcca aatacaaaaa caagtttagc ttaatcgaag
     6301 taggataaac ttcaaaaaag gaaaagcata tgaaatttct ttttattcag gagaagatta
     6361 agttcactgg tgagcagctt ctttcgcaat gggcctataa aaaccatgat ctgttaggag
     6421 atagcttcgt ggctttcatc ggtccctgtg aaatccccga aaaatatatg gtagccttgg
     6481 accacttaaa aaagaaaagc acaatctaca gcgaaaatat gcttcatttt gtaattgagc
     6541 attttgattt ggacctggag aaagcgattt taaggcagaa gcttttacta gccattttga
     6601 aggacaagtt gaatcaccgt ctgggaggag atgtcttaca acgctgggga gatgaccttt
     6661 atgatggaga tgccaaattg actgtttcca ccacagttag gactcctgtt tccaccaaga
     6721 tacatatggg aataaatatc tcttccaaga atgttccggt gaaagctaag ggattggagg
     6781 attattcaat tgatccattt gatattgcgc aggtggtgat gaatcagtat attttggata
     6841 tgcgtcgaat atcggaaagg atcgctaagt cccgttatgt cgaataaatt aacttttaaa
     6901 tttagaaggg ggtaatatgc aggatggaaa gaagttagat aaaataattc tggatgttga
     6961 gaaatgcgag atctcgatca agaagttcat cgagcaggtt caggattatg atatggagag
     7021 gattttaaag aaagtggatg cggagctgat ggatgccctg cataacctga acctggctaa
     7081 aaaacagatg cagccgaaaa aagcagagtg actctgcgaa atgccgtgta agaacataag
     7141 tggggagaga atatctgtat gcttagtgta gtggtaccag tgcttaacga agagaggaca
     7201 attggcaagg ttgttcgctt tgctaagcaa gcccaatctg tggacgaggt tgtagttgtg
     7261 gatgataaat ccatagataa tacagttgaa gaagccaaaa atgctggcgc aaaggtcgta
     7321 attagcacca agatcggtaa gggagcgtct atgcgcgatg gtcttcttgt ttcgaagaat
     7381 gaaataattg ttttcttaga tggggatatc gaaaactacg caccagatac tatagagaag
     7441 atggtgtttc ctatcattgg gggcgaagct gattttgtca aggcgacatt tgagcgtgaa
     7501 gcgggaaggg taacggagtt agtggcaaaa ccacttttaa acttactgtt tcctcaactc
     7561 agccatttct cacaaccttt gagcggtatg attgcaggca ggcgggaaca ttttgagaag
     7621 ataacatttg acaacggcta tggtgtggat attggcatat taattgatat gcacctgctt
     7681 ggggcaaaga tagtagaggt caatatcgga gcaataaaac acaagatgaa gctctggcga
     7741 gagttaggtc gtatgtccag agaggttgca gaggccattc tgaaaaaggc agtcaataac
     7801 ccgaatatca gtctcgattc cttaggcaca attaacatca tccgagacca aatggaattc
     7861 gctttaaaag aaactctctc aactctcaag aagatggtca tattcgatat ggataacaca
     7921 gtcctagatg gtcggtttat cgataaagtt gccctcacct tcggtttcca agaacagctc
     7981 ctcgatatca taacgaagaa ccaagagcca tatctacgta caaaactaat agccaaactt
     8041 tttaagggct taaacatctc tcagctgctc agcgtgacgg acgatattcc tatcgtttcc
     8101 gatacgatag aggtagttaa agagttaaaa aaacgtagtt acattgttgg catcatcagc
     8161 gatagttatg attgcattgc cgcccacatc aaaaataagg ttggagcaga tttttcttta
     8221 gccaacgaac tcgaattctc tcaaggtatt gccacaggcg aagtcaagat tccatctttt
     8281 tttatgaaac aagataaaag catctgtaat catagttttt gtaaaagcaa tgcacttttt
     8341 caagttgctg aacaatatgg gattgagctt tccaatataa ttgcagttgg agatagcgaa
     8401 tccgatatat gcgtggtcaa atatgctggc ataggagttg ccttctgttc gagcaacagc
     8461 atcttaaatg ccattgcaga ccagcggatt gaaactcggt cttttaaaca gatactcgat
     8521 tttgccatat agatctttta tctaacgata agcccatagt gctttgtcaa ctatagcaaa
     8581 cgcattaaat gttgttacat attagagcaa tatgcaatct atcttgaaat ccccctccct
     8641 tcgactttgc tcaggatggt gagtatatcg aaccatcaaa tccccctttt tcaaaggggg
     8701 agacgtcttt tttacccctc tttggaaaag aggcccgctt cgcccgtctc gccgatggct
     8761 cgccgaggcg ggccgaatcg aggcgagggc gaggggagat ttgttgaacg caaattaatg
     8821 cgtttgtatt aacttgagga tttgatgttt gttatatatt ttatagtttc catcggtatt
     8881 cccgccttgc ccacggcttc gccgtgaggc gagccctaaa agagcgggaa tcaccttggc
     8941 ccatcttgag ggcaagatgg gtttaccgtt caattatatt atgcaacttt tatttagcag
     9001 acaaggcacc ataggaacta aatatggagc aggaaaagaa gatatcagag gatgaagctc
     9061 ttttcgttca gctggtgctt atgtttcagg cagcagccat gcaacaattg gggaaattga
     9121 ttaatcctct aaatggaaag acggagcgca atctggagca agctaaattt tccatagata
     9181 tcttagatat gcttaagagc aaaaccgagg ggaatctttc cgaagaggaa aaaaggcttt
     9241 tagagcatgt agtttatgag ctacagatga attacttgga tgagctgaaa aacaaggaaa
     9301 aacaaccaga gaaagatacc cctaaagata agcccacttc aaattgagcc atttttcgct
     9361 tgattttaaa atattaagct ttataattat ataagagaca tcttagtgaa agagcataaa
     9421 aaatacaggg attttgtcgg attgactggt ccagaagtga ggtttggcga ggatttagcc
     9481 aattggacca cactggggat tggaggaagg tgcgacctct tttacgcagc caagaagcca
     9541 gaagatttaa taagggtggt tttggctagc aaaagatttt ccatccctta ttttgttttg
     9601 ggtggtggaa gtaacttatt ggtaagcgat aaggaatttc ggggattggt aataaaaaat
     9661 aaatgtaatc gaataagggt tgaaaagaat aggattatct gccaatcagg cgctctttta
     9721 aaagatatag tctcaattgc caaaacacat tctcttgccg gtttggagtt tgcagttggc
     9781 atctggggaa cagtgggtgg agcggtgtat gggaatgcag gagctttcgc tcaaagcata
     9841 ggagatatat tagagcaggg ggtggtctta acttcttcgg gcaggattga aaacgtaaaa
     9901 aaagatttct ttgaatttgg ctacagggat agcaagctga agtccagtcg agatattttg
     9961 ctttctgtgg tttttaagtt aaaaaaagga aatagtaaga gcatagaaga caaaatgaga
    10021 aagaacctgg cccaaagaag cataagggtt cctaatagga tgaagaccgc cgggtgttat
    10081 tttaaaaatc cacag
//
LOCUS       DASWLN010000171        13259 bp    DNA     linear   ENV 20-FEB-2024
DEFINITION  MAG TPA_asm: Candidatus Zixiibacteriota bacterium isolate
            SMAG_U2949 k141_1118659, whole genome shotgun sequence.
ACCESSION   DASWLN010000171 DASWLN010000000
VERSION     DASWLN010000171.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37960594
            Sequence Read Archive: SRR10376091
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Candidatus Zixiibacteriota bacterium (soil metagenome)
  ORGANISM  Candidatus Zixiibacteriota bacterium
            Bacteria.
REFERENCE   1  (bases 1 to 13259)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 13259)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (26-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/12/2024 08:28:26
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 1,440
            CDSs (total)                      :: 1,416
            Genes (coding)                    :: 1,412
            CDSs (with protein)               :: 1,412
            Genes (RNA)                       :: 24
            rRNAs                             :: 1 (16S)
            partial rRNAs                     :: 1 (16S)
            tRNAs                             :: 21
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 4
            CDSs (without protein)            :: 4
            Pseudo Genes (ambiguous residues) :: 0 of 4
            Pseudo Genes (frameshifted)       :: 0 of 4
            Pseudo Genes (incomplete)         :: 3 of 4
            Pseudo Genes (internal stop)      :: 1 of 4
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..13259
                     /organism="Candidatus Zixiibacteriota bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_1118659"
                     /isolate="SMAG_U2949"
                     /isolation_source="soil"
                     /db_xref="taxon:2053527"
                     /environmental_sample
                     /country="USA: Oregon"
                     /lat_lon="42.99 N 123.63 W"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            complement(<1..1003)
                     /locus_tag="VGB16_06330"
     CDS             complement(<1..1003)
                     /locus_tag="VGB16_06330"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="delta-aminolevulinic acid dehydratase"
                     /protein_id="HEX9917338.1"
                     /translation="MHSRLRKDSKKGGVIDTQILNNSLGRLLNYCEEKNWKGYDPYDG
                     LNTAWRKVLPLKGKIFGILLIQFFKRSPLNLRKLLFVKEDHNPKGLGLFLSAVARLYD
                     HCGEERYKKLAYRFIDLLLEKKSTGHSGICWGYNFDWQSKAFFLPKYTPTVVATSFIA
                     NAFLDAYEVFKEEQFLKIARSSCDFVCNDLNRTLEGENFCFSYSPLDKTTIHNANILG
                     AHLLARTASFTGDDALKKTAGDSIQFVIDHQNSDGSWYYGTQAHHKWIDNFHTGFVLE
                     SLFDYINFASKRELRSNLVRGLKFYCENFFLSDGTPKYYHDRIYPVDIHSCAQSIITL
                     "
     gene            complement(1025..3244)
                     /locus_tag="VGB16_06335"
     CDS             complement(1025..3244)
                     /locus_tag="VGB16_06335"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_010856128.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="bi-domain-containing oxidoreductase"
                     /protein_id="HEX9917339.1"
                     /translation="MKQVLQNNKTGELKVEEVPHPILMRRGVLARNEYSLISKGTERT
                     KVDFARKSTVAKAKSRPDLVKQVLKNAKKEGWATTFKKVMNKLESSSPLGYSSAGEVM
                     VVGDLVEGIKVGDKVACAGAGYANHAEIISVPVNLCAKIPENVSCRQACFTTLGAIAL
                     QGIRQAQVNLGENVAVIGLGILGQLTVQMLKASGCQVFGMDIDEEMVNLAKNSDADSG
                     LVIGKEDVASKVINLTCGHGVDVVIITAGTTSNEPIELAGDICRDKGRVVIVGAVNTD
                     VPRKNFYDKELDLRFSRSYGPGRYDHLYEEMGIDYPFGYVRWTEKRNMEEFLNLLQQG
                     KVNTEKLVTHEFKVQDAEKAYDLIMKGDEKSLAVLLKYEPFSETKPRIYLKDVSPHAG
                     KGISAVNIGFIGAGNFANNNLLPHLKKMKEVKLKGVVTSTGISAKDVARKFGFEYCSS
                     DVGQILEDEEINCIFIATRHNLHSPLVCQALKAGKAVFVEKPLATSEVELEEIKRTLE
                     QTRGRILVGFNRRFSPACQLLKKHFQDRTTPLVMTYRVNAGFIPASHWIHDPEEGGGR
                     IIGEVCHFVDLLSFFSNSEVSSIFALSTNDPRKTPLTNDNLVISLKFTDGSVGSVLYT
                     SLGDTSYSKERVEIFGENSVGVIDNFQRTSIVRNGKELRLKHSGSDKGHKEELEAFIQ
                     AVKQDKPMPISPEEIINTTQATFKMIESLQRNSPAEIQLLNSNEIRMKKVECPSGRF"
     gene            complement(3222..4682)
                     /locus_tag="VGB16_06340"
     CDS             complement(3222..4682)
                     /locus_tag="VGB16_06340"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="phenylacetate--CoA ligase family protein"
                     /protein_id="HEX9917340.1"
                     /translation="MTTFSLELKTIYKRSPSWVKELSGFVCPHLPSLFIYGREFVSHK
                     TLLKKSQWWSKDQLAQYQITSLRRLIDHAYQNVPYYRKLLTERGLKPVEFSSFADLKK
                     LPFLTKEIIQEHFEDMIAKNIPRKKMKLVTTGGTSNNQLKFHIEEESSLKEWAFMTTQ
                     WERINFRLNDKRLVLRGTPVDSSTNDGLWEYDPLTRECRLSVYHLTEKNLFKYVEVMK
                     RFSPDFIHGYPSAVAIFARFILDNEIHNLPPLKGVLCGSEAVLDHQRKLVEKAFKTRL
                     FSWYGQSEKVILASECETNFSYHIFPEYGITELVDENENQITEPNRVGELVGTGFINY
                     AMPFIRYKTGDWASFEQGYCECGRKYPLINKIAGRRLTEFLVGRQGNPISMTAINMHD
                     DTYENVRQFQFYQDRPGITTLKIIRGKKYSQKDTQRLLSRLEQKLKDNLEIRISFVES
                     LPLSPSGKHRFVAQKIPIENLKQLVKGALDETGLTE"
     gene            complement(4697..6190)
                     /locus_tag="VGB16_06345"
     CDS             complement(4697..6190)
                     /locus_tag="VGB16_06345"
                     /inference="COORDINATES: protein motif:HMM:NF015948.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF362 domain-containing protein"
                     /protein_id="HEX9917341.1"
                     /translation="MSRFDLNNSQVAMSKTQKAIYPSHPPFHPSEHYPESSFPIQLST
                     EKNYVYAALRENLRLLGLDRRNYGKRAWNPLGEIVRKGDNVIIKPNFIRQSHLLNEDW
                     EYVITHGSVIRAVLDYVYLALEGEGKIIVADGPQTDSDFSSICLKTGIHPIIDFYRKE
                     AGFKIELLDLRQERWIQKGNIIEERIKLKGDPKGYTKINLAEESEFAGYGLSGKFYGA
                     DYFSEQTIWHHSNGRHEYLVSSTLLDADVVINLPKLKTHKKAGVTLSLKNMVGINGDK
                     NYLPHFTEGTPSEEGDQFDSSALKNKLEGKYRKILYRFLECAGGRGKVLARGTRRAGE
                     LIFGSTKKTIRSGNWYGNDTIWRTILDLNKILFYSDASGHMRLLSPKRYLSIVDGIIA
                     GEGDGPMAPDPKACGILLTGFNPVAVDIVGATLMGFDFNKIPQLKNAFQIKNFKLVPF
                     QPEKIELITSNGNSNRRLFEIDHQDILPFKPHFGWKDHIELDSLKTY"
     gene            complement(6243..7373)
                     /locus_tag="VGB16_06350"
     CDS             complement(6243..7373)
                     /locus_tag="VGB16_06350"
                     /EC_number="2.4.-.-"
                     /inference="COORDINATES: protein motif:HMM:NF012744.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016757 - glycosyltransferase activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="glycosyltransferase family 4 protein"
                     /protein_id="HEX9917342.1"
                     /translation="MQRKTLIIGPLPPPYHGVAVMIQWLIEGLTKRNRLSFVHLNTQD
                     PQKNKGFGKFSLRNSWFALKDIFVLFKLLLREEIEVVYIPVSQNFWGFARDSLFVLIS
                     GLVFGRKVVIHLHGGYFKTFFNQGTWLRKKYIKFVFKYVDRGIVLGYCLKYLLEDVLP
                     KDKIDVVCNGVDVQPFDSVESQRQEKRKFKILFAGVLEESKGFFDLIKAVPTLARHYP
                     DIEVLVAGRWQANGLKNIVESYVRKNDLEKYVRFVGVVTGEEKMNLFKSSDVFVLPTY
                     YHLEGQPVVILEAMAAKLPVITTNRGSIKEMITDRENGFLISPRSPGEIAEKVALLIE
                     DKSLKEKMGELNHKKVKERFTLDGYVDGVMQVIDKSANGKRL"
     gene            complement(7407..8090)
                     /locus_tag="VGB16_06355"
     CDS             complement(7407..8090)
                     /locus_tag="VGB16_06355"
                     /EC_number="2.1.1.-"
                     /inference="COORDINATES: protein motif:HMM:NF024880.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008168 - methyltransferase activity
                     [Evidence IEA];
                     GO_function: GO:1904047 - S-adenosyl-L-methionine binding
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="class I SAM-dependent methyltransferase"
                     /protein_id="HEX9917343.1"
                     /translation="MQKKEYYKLLQESRTFESIQFGSRVWEASKMLKEGENFLDIGCG
                     TGTLLYQAKRYYSDVYGLDITISKELKSQNSQFKLLEVDIDKHGLPFEDGFFDTISAL
                     DVIEHVFDPVVLLEETYRVLKEDGQVIYTTPNVRSLKHLFALIFKGKFPKTSNERIGY
                     DGEHLHYFTHCDIKELFQRTGFREVKTTGLMTLDGRFATLKRLLRKGLNISSIKEFLS
                     PGIVIKARK"
     gene            complement(8068..9240)
                     /locus_tag="VGB16_06360"
     CDS             complement(8068..9240)
                     /locus_tag="VGB16_06360"
                     /EC_number="2.4.-.-"
                     /inference="COORDINATES: protein motif:HMM:NF012744.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016757 - glycosyltransferase activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="glycosyltransferase family 4 protein"
                     /protein_id="HEX9917344.1"
                     /translation="MINNRKTILVTNIMSPYRIATFNQLASKYGKAFKVVFCKQNDHY
                     RKWKLSERDQLKFDFCVLNDFSIKLKNWEIHLANGLLSELIREKPFLVITEGFGFPTI
                     FSFLYASIFDRKLILFSAETQHVAESYSRFRYLLRKLMVRNAQGFIATGTQAKEYLVS
                     LKAPEEKIFLSYFSIDPELLGLDTNSVAETKKHSEEPKRILYVGKFTERKRVDLLISA
                     FRQVKKRLPNSELWLVGEGEEEEKLKKLSQETKDIFFEGHKQYGELLPYYLNSDLFVL
                     PTLKDIWGLVVNEAMMCGLPVICSKYAGCCQDLIKQGENGLIVDPENPKELSEAISQL
                     LSKDGNLSRFGRRSREIIKDFTTERTTGGFIRAIESVASSRNSGSVGRKDAKERIL"
     gene            complement(9237..10448)
                     /locus_tag="VGB16_06365"
     CDS             complement(9237..10448)
                     /locus_tag="VGB16_06365"
                     /EC_number="2.4.-.-"
                     /inference="COORDINATES: protein motif:HMM:NF012744.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016757 - glycosyltransferase activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="glycosyltransferase family 4 protein"
                     /protein_id="HEX9917345.1"
                     /translation="MKVTISAARADHSYKTALATQNAGLLEKYYTTLYFKPESLWGSF
                     LVSFSDRFGSLKSLLNRLKSNRYLKGLESRNVVSITTPELLGRAWRHIPLLRNLFPWN
                     KLKDNLFDLLVSREIKDCDIFHGWLGYSLNTLSKFGESRTKILIDVYTAHPEHRRKLL
                     SEEYEKFNLNCRIPDEPLWKKYVKEMELADYLLAPSEYVYNSCTSEGVPEEKIKVIPF
                     GVDLQRFKPVKKSDSLFRLLFVGMISINKGVYYLLEAYKQLHLPKSELVLIGHLQPEF
                     KNIITKYNGLFRHLPHVPNGQLLNWYGGSSAFVFPSLTEGSAMVTYEAMACGIPSIVT
                     ENSGSVVRDKVDGFVIPIRDIESLKEKILFFYENEEKRKEMGKSARERAEQFTWERYG
                     NQLIKFYERIV"
     gene            complement(10445..11905)
                     /locus_tag="VGB16_06370"
     CDS             complement(10445..11905)
                     /locus_tag="VGB16_06370"
                     /inference="COORDINATES: protein motif:HMM:NF016800.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="O-antigen ligase family protein"
                     /protein_id="HEX9917346.1"
                     /translation="MKTIALAPHNSGTSYLLAIVLGITGSLLLVLFSKYLFFALGFFI
                     LLVMLLYLLNDPVKGVLLFLSTKCIFDMFWFIRIPVTVLIDLNVQRAIGILFPLAILG
                     IFVLKERNFFKAFSTTLGKIILLYIAFNALAIFLASSAPRALVQFSQLIGSFILFFVL
                     GVTAFTEKDLRALLHYLLVFLWIPLVIAFLETFGLVSFSSFIQNAQYVYSSSGGVYAA
                     RLVGLYSHPFDVVRYLVVAFPLALWMMSTEANPFKKLFYNITFLFLSFAMWRTFYRTG
                     WIIFALQLVFWLKMRKSHKAMLAVILICVCVVLMNLTFFTSFYDTLLVLLSPSGEDVM
                     TAFSGRFVIWSFHLAKFAESSRLAQLFGHGLGSEIAIYHEMSDVLSSNRETSHSDFIR
                     NLSEIGIAGLGLYLLVLILLGKELLSSIKREEDPSFRAFGQAIFLIFIGFLLLSNVAA
                     PALNPSIAWYLWGFAGVVIGRKFLNNKLQQKQDHKK"
     gene            complement(11960..13207)
                     /locus_tag="VGB16_06375"
     CDS             complement(11960..13207)
                     /locus_tag="VGB16_06375"
                     /inference="COORDINATES: protein motif:HMM:NF014051.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="flippase"
                     /protein_id="HEX9917347.1"
                     /translation="MADRATILKNFFSLFFGQVVARGLNFLAIAYLARALGSDGFGMI
                     NFAQAILAYLMVFCTLGLDTFGTREIAANHKIKKESFIGNLLALRMGLALISLLGLGI
                     FILTIDKPTLEKEVIFAYGLYLFPFVFYLGWVFCGIERMEYIGIAAIVSRLAFLATVL
                     ILIKDVGNVTLVPYLWFTSAVVEGLFLIFSYRKFGSIKLDVHLAEWKLFIKESVWIGI
                     STILLQIYSNFNLVMLGFMKTNHDVGLYSAASKIILFIIAIGAYYNNSIFPVISRYAQ
                     DSKLKLKRFIHRSTILIAVGGVGLWILLFIFARQIIVLAYGDSYRGSVLTFQILSLTI
                     VTGIIRLIYISVLVAFNKIRESLWGVGIGCGINVTLNFLLIPHYGIAGAAVATLIGEI
                     IITIAIARIALRFLETENMIHPG"
BASE COUNT         3790 a         2999 c         2478 g         3992 t
ORIGIN      
        1 ccaaagtgat gatcgattgg gcgcagctgt ggatatccac ggggtaaatc ctatcgtggt
       61 aatacttggg agtgccatcg gataagaaaa agttctcaca atagaattta agtcctctca
      121 caagatttga tctcagttca cgtttagagg cgaaattgat atagtcgaac aaactttcca
      181 aaacaaaacc ggtatgaaaa ttgtcgatcc atttatgatg agcttgggtg ccgtaatacc
      241 aggacccatc actattttga tgatcgatta caaattgtat tgaatcacca gctgtttttt
      301 tgagtgcatc atcaccggtg aatgaagctg ttcgggcaag aagatgcgcc cctaaaatgt
      361 tggcattgtg aatggttgtt ttatccaaag gtgaatagct gaaacagaag ttttctcctt
      421 caagagttct attcagatca ttgcaaacga aatcgcaaga gcttcgcgcg atctttaaaa
      481 actgttcttc tttgaaaact tcgtaggcat ccaggaatgc gttagctata aaagaggttg
      541 ccactaccgt gggagtatac ttgggtagaa agaaagcctt agattgccaa tcaaaattgt
      601 atccccagca tatgccggag tggcctgtgc ttttcttctc caagagcaga tcaatgaacc
      661 gataagctaa ctttttgtat ctttcttcgc cgcaatggtc gtataatctt gcgactgctg
      721 atagaaaaag ccccaaaccc tttgggttat gatcttcttt gacaaaaagt agctttctta
      781 gattaagggg agatcgcttg aaaaactgaa taagcaaaat gccaaagatc tttcccttta
      841 agggcaaaac ctttcgccag gctgtattta gcccatcata aggatcatag cccttccagt
      901 ttttctcctc acaatagtta agaagtcttc ctaaagaatt atttaagatc tgagtatcga
      961 tgactccacc tttttttgaa tcttttctca atctggagtg caaaaactta gttttttgcg
     1021 ttttctaaaa ccgcccagat gggcactcca ctttcttcat tcttatttca ttagagttga
     1081 gtaattgtat ctcagccggg ctgttcctct gtaaagactc gatcatttta aatgtagcct
     1141 gagttgtgtt aattatttcc tctggtgaga taggcatcgg tttgtcttgt ttaactgcct
     1201 ggatgaacgc ctctaattct tctttatgtc ctttgtctga tccggaatgt ttgagtctta
     1261 actcctttcc atttcttact atagaggtcc tttggaaatt gtctatcacg cctactgaat
     1321 tttccccaaa aatctctact ctctccttag aataagatgt gtcacccaac gatgtataga
     1381 ggacggagcc aactgaacca tccgtaaatt taagggagat gaccagatta tcattagtaa
     1441 gtggagtttt tctggggtca ttggttgaca aagcaaagat agatgaaact tctgaattgg
     1501 aaaagaagct cagaagatca acaaaatgac aaacctcgcc aattatcctc ccacctccct
     1561 cttctggatc gtgtatccag tgacttgccg gtatgaagcc agcatttacc cgataagtca
     1621 tgaccagagg tgtggtccta tcctggaaat gcttttttaa caattgacac gctggtgaga
     1681 aacgacgatt gaatcccact aatattcttc cccgtgtctg ctccagagtt ctttttattt
     1741 cttccaactc gacttcgctt gtggctaagg gcttctccac aaataccgct ttcccggctt
     1801 tcaaagcttg gcagaccagt ggagaatgca agttatgcct ggtggcgatg aagatgcagt
     1861 taatctcttc atcttccaga atctgaccta catctgagga acaatactcg aaaccgaact
     1921 tccttgccac atcctttgcc gaaattccgg tggaggtaac cacccctttt aattttactt
     1981 ctttcatttt tttcaaatgg ggcagaagat tgttgttggc aaaatttcct gccccgataa
     2041 atcctatgtt caccgcggag attcccttgc ctgcatgggg agagacatct tttaaataga
     2101 ttctgggttt ggtttcagaa aaaggctcat actttaaaag cactgccagg gatttctcat
     2161 cacccttcat tatcaagtca taggcttttt ctgcgtcctg gactttgaat tcatgagtaa
     2221 ccaacttttc tgtgtttacc tttccttgct ggagcagatt caaaaattct tccatgttcc
     2281 ttttttcggt ccagcgaaca tatccgaagg gataatctat gcccatctct tcataaagat
     2341 gatcatatct gcccggacca taggatcgag aaaatcggag gtccagctcc ttatcataga
     2401 aattctttcg gggcacatcc gtatttacgg cacctacaat gacgacccta cctttgtcac
     2461 gacagatatc tcctgccagc tcgataggct cgttgcttgt ggttcctgcc gttataatta
     2521 ccacatccac accgtgacca caagttaagt taattacttt agatgctaca tcttcctttc
     2581 caatgaccaa accagaatca gcatctgaat tttttgctaa attgaccatt tcttcatcta
     2641 tatccattcc aaagacctga cagcctgaag ctttgagcat ctggacagtt aactgcccca
     2701 atattcctaa cccaataact gcaacatttt cacccaaatt gacttgggct tgtcttatcc
     2761 cctgtagggc tatagcaccc aaggtggtaa agcaggcttg acgacaactc acattttccg
     2821 gaattttcgc acacaagttc acgggaactg agatgatctc agcatgattg gcataaccag
     2881 caccagcgca ggcgacctta tcccctacct ttattccttc caccaaatct cccactacca
     2941 ttacctcacc ggctgaactg tagcccaaag gtgatgagga ttctaattta ttcatcactt
     3001 ttttgaaagt tgtcgcccaa ccctcttttt ttgcattttt caaaacctgc ttaaccagat
     3061 ccggtcggct cttagcttta gccaccgtgc tctttctggc aaaatccacc ttcgtccttt
     3121 cagtcccctt ggagatcaaa gaatattcgt ttctcgccaa gacccctctg cgcataagga
     3181 tcggatgtgg cacctcttca actttcaact ctccagtttt gttattctgt aagacctgtt
     3241 tcatccagag ctcctttcac taattgctta agattttcga taggtatctt ttgggctacg
     3301 aatctgtgtt ttccagatgg actcaaaggt aaactctcca caaagctgat tcggatttcc
     3361 agattatctt tgagtttttg ttccagtcgg ctaagtaacc tttgggtatc cttctgagaa
     3421 tatttctttc ctcggattat tttcaacgtg gtaatgccgg gtcgatcctg atagaattga
     3481 aattgtctca cgttttcata ggtgtcatca tgcatgttta tggcagtcat ggagatcgga
     3541 tttccttgtc tccccacaag gaattctgtt aacctcctcc ctgcaatttt gtttataaga
     3601 ggatatttcc gtccgcattc acaatatcct tgctcaaaac tcgcccagtc tccggtctta
     3661 tatctgatga atggcatagc gtagtttata aagcccgtac caaccaactc tccaactcga
     3721 ttaggttctg tgatttggtt ttcattttca tccaccagtt ctgtgattcc gtattcggga
     3781 aagatatgat agctgaagtt tgtctcgcat tcgctggcta atatgacttt ctcactttga
     3841 ccgtaccagg aaaacaatct ggttttgaaa gctttttcaa ctaactttct ctggtgatct
     3901 aagacagctt cggatccaca taaaactcct ttgaggggag gtaagttatg gatttcatta
     3961 tctaatataa atcgagcaaa gatagccact gcagagggat agccgtgtat aaaatctgga
     4021 gagaatcttt tcattacttc gacatacttg aataggttct tttcggttaa gtgataaacc
     4081 gataatcgac actctcgggt aaggggatca tattcccaca gaccatcatt tgtagaagaa
     4141 tcgacggggg tccctctcag aactaatctt ttgtcattta atctgaagtt tattctttcc
     4201 cattgagtgg tcataaaagc ccattcttta agcgaagact cttcttctat atggaacttc
     4261 aattgattat tggaagtacc tcccgtcgtt accaatttca tttttttccg aggaatattt
     4321 tttgcaatca tatcttcaaa gtgctcctgg atgatctcct tggtcaagaa agggagcttt
     4381 tttaaatctg cgaaactcga aaattcgaca ggtttcaggc ctctctcagt caacagcttt
     4441 cgataataag gtacattttg atatgcgtgg tcgatcaacc ttctgaggga ggtaatctgg
     4501 tattgggcaa gttgatcttt gctccaccat tgggattttt tcaacagggt tttatgagaa
     4561 acgaattccc taccatagat gaaaagagaa ggaaggtgag ggcacacaaa cccgcttagc
     4621 tccttgaccc aagatggact tctcttatat atggttttta attcaagaga aaaagtggtc
     4681 atttccaatg cccttactaa taagttttga gcgaatcgag ctcaatatgg tccttccagc
     4741 cgaaatgtgg cttaaaagga aggatgtcct gatggtcgat ttcaaacaat cttcggtttg
     4801 agtttccatt actagtgatt aattctatct tttccggttg gaaaggcacc agcttaaagt
     4861 ttttaatctg gaaagcattt ttcagttgcg ggattttatt gaaatcaaat cccataaggg
     4921 tggcacccac tatatccacc gccaccggat tgaatcccgt caataaaatt ccgcatgctt
     4981 taggatcggg agccatgggt ccatccccct ctccggcaat gattccatcc acaatagaaa
     5041 gatacctttt gggagacaga agtcgcatat ggccagaggc atcggagtaa aaaagaatct
     5101 tgttgaggtc aagaatggtt ctccaaatgg tgtcatttcc ataccagttt ccacttctta
     5161 tggtcttttt cgtgctaccg aaaatcaact ctccggctct tctggtccct ctcgccaaga
     5221 cttttcccct accgccagcg cattctaaga atcgatagag gatttttctg tactttcctt
     5281 ccaatttatt ctttaaagca gaactatcaa actgatctcc ttcttcagag ggagttcctt
     5341 cagtgaaatg gggcaaatag tttttgtcgc cgtttattcc caccatattt ttcaaactca
     5401 atgttacgcc tgctttttta tgagttttga gtttgggaag atttatgacc acgtctgcat
     5461 ccagaagtgt ggatgaaacc aagtattcat gtcttccatt agagtgatgc cagatggttt
     5521 gctcggagaa ataatctgcc ccatagaatt ttccgcttaa accatatccg gcaaactcgc
     5581 tttcttcggc taaatttatt ttggtgtatc ccttggggtc ccctttcaat ttaattctct
     5641 cttctatgat attgcctttt tgaatccacc tctcttgcct aagatccagc agttcaattt
     5701 tgaagccagc ctccttccta taaaagtcga ttatgggatg gatgcctgtc tttaaacaga
     5761 tagaggaaaa atccgaatca gtttggggtc catccgctac gattattttc ccctcacctt
     5821 ctaaagccag ataaacatag tctaaaaccg cccttatgac ggagccatgg gtaataacat
     5881 attcccagtc ttcgtttaac agatgagact gcctgataaa attcggcttt attatcacat
     5941 tgtccccttt tcggacgatt tcccctaacg gattccaagc tcgtttacca tagttccttc
     6001 gatctaaccc taaaagtcta aggttttccc tgagtgcggc gtaaacataa tttttctcag
     6061 tcgaaagctg aatgggaaag gaggattcag gatagtgctc tgaagggtga aagggggggt
     6121 gggaaggata gatagccttt tgagttttgc tcatcgcaac ctgtgaattg ttcagatcaa
     6181 atcttgacat tttcttaaat ccctttgcta aattatcgac atagacgcag atcgtagatt
     6241 ctttatagtc ttttgccatt ggctgattta tctattacct gcatcacacc atccacataa
     6301 ccatccagag tgaatctctc ctttactttt ttgtggttta gctcacccat cttctctttc
     6361 aagcttttat cctcaattaa cagagcaacc ttttctgcaa tctcgccagg agaacgaggg
     6421 gaaataagaa agccattctc tcgatcagtg atcatctctt taatgcttcc ccggttcgtg
     6481 gttatgaccg gcaattttgc ggccatcgcc tctaaaatga ccaccggttg cccctccaaa
     6541 tgatagtaag tcggcaaaac aaacacatcc gagcttttga acaaattcat cttttcttcc
     6601 ccagttacaa ctccaacaaa tctaacatat ttttccaaat cattcttccg aacataagat
     6661 tcaactatgt ttttcagtcc atttgcctgc cagcgtccgg cgacgagaac ctctatatcc
     6721 ggataatgtc ttgcaagcgt tggcaccgct ttgatcagat caaaaaatcc tttggattct
     6781 tctaaaactc cggcaaaaag tatcttgaac ttcctctttt cttgcctctg gctttcaacc
     6841 gaatcaaagg gctgtacatc cacaccatta cacaccacat cgattttatc tttgggtaac
     6901 acatcttcta aaagatattt caagcaatag cccaaaacta tgcctctatc cacatattta
     6961 aacacaaact taatgtactt ctttctcaac caggtgcctt ggttgaagaa agttttgaaa
     7021 tatccaccat gcagatgaat gaccaccttt cgtccaaaga caagtcccga aatcaaaacg
     7081 aagaggctat ctctggcaaa cccccagaaa ttctgggaga cgggtatgta aaccacttcg
     7141 atttcttctc tcaagagaag cttgaaaagg acaaaaatat cctttagggc aaaccaactg
     7201 tttcggagac tgaatttccc gaaacctttg ttcttttgag gatcctgggt atttaagtgg
     7261 acgaagctga gtctatttct tttcgtcaat ccctcaatta accattgtat catcactgcc
     7321 accccatgat aggggggagg caaaggacct attatgagtg tctttctttg cataaccttc
     7381 ctctgaatgc aaaaccgcct tagatcttat tttcttgctt ttatcacaat tccgggcgac
     7441 aaaaactctt tgatagagct tatattcaaa ccttttctca ataacctttt gagggtggca
     7501 aatctgccgt ccagggtcat aagtccggtt gttttcactt ccctgaagcc ggtcctttgg
     7561 aacaactctt tgatatcaca atgggtgaaa taatgcagat gctctccgtc gtatccgatc
     7621 ctctcgttgg aggttttagg aaatttccct ttgaatatta acgcgaagag atgttttaaa
     7681 gacctcacgt ttggagtagt gtagatgact tgcccatcct cttttagcac gcgataagtt
     7741 tcttcaagta aaactaccgg atcgaaaaca tgttctataa cgtccaatgc tgagatggta
     7801 tcaaagaaac catcctcaaa gggaagtcca tgtttatcta tatccacttc caagagtttg
     7861 aattggctat tttggctttt aagctctttt gagatggtta tgtctaaccc ataaacatca
     7921 gaataatatc tcttggcttg atacaaaagg gtccccgtgc cacaaccaat atccagaaaa
     7981 ttctcaccct cttttaacat cttgcttgct tcccacaccc gagacccaaa ttgaatgctc
     8041 tcaaaggttc tactttcttg caggagctta taatattctt tcttttgcat ctttcctccc
     8101 gacggatcca gaattccgac ttgaagctac agattctatg gctcggatga accctccggt
     8161 cgtcctctcg gtggtgaaat ccttaatgat ctcccgcgat ctcctgccaa aacgggaaag
     8221 atttccgtct ttcgacaaaa gttgagaaat agcttcggat aattcctttg ggttctcggg
     8281 atcgacgatc agaccgtttt ctccttgttt tatcagatct tgacagcatc cggcatattt
     8341 ggaacagata actggcagcc cacacatcat ggcttcattc accactaaac cccatatatc
     8401 ttttaaggtg ggcaaaacga aaaggtccga attaagataa taggggagaa gttccccata
     8461 ctgcttatga ccttcaaaga agatatcttt ggtttcctga gataatttct ttagcttttc
     8521 ctcctcttct ccttcaccta ctagccaaag ttcgctattg ggcaaccttt ttttgacttg
     8581 cctaaaagcc gaaattaaaa gatccactcg tttgcgctcg gtgaatttcc ccacgtagag
     8641 aatcctctta ggttcttctg aatgcttttt cgtttcggcc acgctattgg tgtccagtcc
     8701 caaaagctct ggatcgatgg agaaatatga caaaaagatt ttctcttccg gagctttcag
     8761 agagacaaga tattccttcg cttgagtccc tgtggctatg aacccttggg catttcttac
     8821 cataagcttt cttagaaggt agcgaaacct gctataggat tcggcaacat gttgggtctc
     8881 ggcagaaaac agtataagtt tcctatcaaa tatgcttgcg taaagaaaac taaatattgt
     8941 agggaaacca aatccttccg taatcacaag gaagggcttt tctctgataa gttccgataa
     9001 aagaccattt gccaaatgta tttcccaatt cttgagtttt atgctaaaat cgttcaatac
     9061 acaaaaatca aatttcagct gatctctctc ggatagcttc cattttctgt aatgatcgtt
     9121 ttgtttacag aaaactacct tgaaagcttt cccatatttt gatgccagtt gattgaaagt
     9181 agcaatccta tatggtgaca ttatgttggt tactaatatt gtctttctgt tgtttatcat
     9241 actatcctct cgtaaaattt tataagttga ttgccatatc tttcccatgt aaattgctcg
     9301 gcacgttctc tggcagattt tcccatttct tttctcttct cttcattttc ataaaaaaac
     9361 agaatttttt ctttcaagct ttcgatgtcc ctaatgggta ttacaaagcc atccactttg
     9421 tctcttacca cactccccga attttcagtt acaatggagg gaatcccgca agccatagct
     9481 tcgtaggtca ccatggcgct tccttcggtc aaggagggaa aaacgaaagc ggatgaaccc
     9541 ccataccagt ttaaaagttg accgttcgga acgtggggta aatgcctgaa taaaccgtta
     9601 tatttggtaa ttatattttt gaactccggc tgcaggtgac cgatcaaaac cagctctgac
     9661 tttggcagat gaagttgttt gtatgcttct aaaagataat aaactccttt gttaatagat
     9721 atcatcccca caaagagtag tctgaaaaga ctatcagatt ttttcacagg tttaaatctc
     9781 tgtaagtcaa cgccgaaagg aatcaccttt attttctctt ctggaactcc ttctgaggtg
     9841 caactattat atacatactc agagggagcc agtagataat ccgctaattc catttccttt
     9901 acgtatttct tccacaaggg ctcatcaggt attctacagt tcagattaaa cttctcatat
     9961 tcctcgctga gtagtttcct tctgtgctcc ggatgagccg tataaacatc tatcaatatt
    10021 ttggttctgg attctccaaa tttacttagt gtatttaagg aataacccaa ccatccatga
    10081 aagatgtcgc aatcctttat ttctcgagag accagcaggt caaatagatt gtcttttaat
    10141 ttgttccagg ggaataggtt tctcaacaat gggatatgtc gccaagccct tcccagaagc
    10201 tctggagtag tgatggaaac cacgtttcgt gactccaatc ctttcaggta tcggttgctt
    10261 ttaagccgat tgagtaaact ttttagggag ccgaatctgt ccgaaaaaga gactaaaaac
    10321 gatccccaca aactttcggg cttaaaatac agtgtggtat aatatttctc cagtagtccc
    10381 gcattctgtg tcgccaaggc ggttttatat gaatggtctg ctcttgccgc tgaaatggtt
    10441 actttcattt cttgtggtcc tgcttttgtt gcagtttgtt attcaaaaac tttctcccaa
    10501 taactacccc tgcaaatccc cataagtacc aggcaatact tggatttaaa gctggagctg
    10561 ccacgttgct caaaagcaaa aatcctataa atatgagaaa gatcgcctgc ccaaaagctc
    10621 gaaaggatgg gtcctcttcc cgtttgatac tggataacag ctcttttccc aataaaataa
    10681 gaaccaaaag ataaagaccc agcccggcta ttccgatttc acttagattt cggatgaagt
    10741 cactgtgact ggtttcccgg ttagaagaga gcacatcact catctcgtga tagatagcga
    10801 tttctgaacc caggccgtga ccaaaaagct gcgcaagcct tgaactctcg gcgaatttgg
    10861 ctaaatgaaa gctccagata acgaatctac cgctgaaagc agtcattacg tcttcacccg
    10921 atggagacaa gagaactaaa agggtgtcgt aaaagctggt aaaaaaggtg agattcatta
    10981 agaccacgca tacacagatt aagatcacag ctaacatagc cttatggctt tttctcattt
    11041 tgagccaaaa aaccaactgc agagcaaaga ttatccatcc ggttcggtaa aaggttctcc
    11101 acatagcaaa agagagaaag agaaaagtga tattgtaaaa gagtttttta aaaggatttg
    11161 cttcggtaga catcatccac aaagccaaag gaaaagctac aaccaagtag cgcaccacat
    11221 caaagggatg agaataaagc cctaccagac gagcggcata aactccaccc gaagaagaat
    11281 agacatattg tgcgttttgg ataaaggagc taaaggatac caagccgaaa gtttccaaaa
    11341 atgcaattac caacggaatc cacagaaaga ctaagagata atggagtaat gctcttaagt
    11401 ctttctcagt aaaagctgtt actcccagaa cgaaaaacaa aatgaagctt cctattagtt
    11461 gggagaactg aactagggct cggggtgcgc tcgacgcaag gaatatcgcc agggcattaa
    11521 aagctatgta cagaaggata attttgccta aagtagtact gaatgcctta aagaaattcc
    11581 tctctttaag aacaaaaata cccaaaatcg ccagagggaa aagaattcct atggctcttt
    11641 gcacattcaa atcgattaag acggtaactg gaatcctgat aaaccagaac atatcgaaga
    11701 tgcatttggt ggaaagaaag agcaagaccc cttttaccgg gtcattcaaa agataaagga
    11761 gcatgaccaa gagaataaaa aaacccaaag caaagaacaa gtacttcgag aacaacacca
    11821 aaagcaaact tccggttatt cctaagacta tggctagaag atagcttgta cctgagttat
    11881 gtggggctaa ggctatcgtc ttcatttcgt actcctcatt ttattaccaa attattaaag
    11941 cgttcttgaa actctccggt catccaggat gaatcatatt ttcagtttct aaaaacctca
    12001 aagctatcct ggcaatcgct atggttatga tgatttcacc gatcaaggta gcaacagctg
    12061 ctccggcaat tccataatga gggatcaata agaagttcaa ggttacatta atcccgcagc
    12121 cgatacctac tccccaaaga ctctctctga ttttattaaa agccactaat acgctgatgt
    12181 aaatcagtcg tataattccg gtcactatgg tcaaggataa tatttgaaaa gttaaaacac
    12241 ttccccggta actatcccca taagctaaaa ctattatctg tctggcgaaa ataaaaagta
    12301 agatccataa accgactccg ccaaccgcaa tgaggattgt actcctatgg ataaatcgtt
    12361 tcaatttgag cttggagtct tgagcatatc tagatatcac gggaaagata gagttattat
    12421 aataagctcc gatagctata atgaataaaa tgattttaga ggccgcgctg taaagtccca
    12481 catcatgatt ggttttcata aaaccaagca tgaccaggtt aaaattcgaa tatatctgca
    12541 gcaaaatggt tgatatccca atccagaccg actccttgat aaataatttc cattctgcaa
    12601 gatgaacatc aagttttatg ctgccgaatt ttcggtaaga aaaaatcaga aaaaggccct
    12661 ccaccactgc cgaagtaaac cagagatagg gaaccaaggt cacattaccg acatctttta
    12721 ttaagatcaa aacggtggct aaaaatgcga gccgagaaac aatcgccgct atcccgatat
    12781 attccatccg ctcgatccca caaaagaccc atcccagata gaaaacaaaa gggaaaagat
    12841 aaagcccgta ggcaaatatg acctcttttt ctaaggttgg tttatctatg gttaatataa
    12901 aaattcccaa acccagaaga gatattaaag ctaaacccat cctcaaagcg agcagattcc
    12961 caatgaagct ctcttttttg attttatgat tagcggcaat ttccctggtt ccaaatgtat
    13021 ccaatcctaa agtgcagaaa accataagat aagccaaaat cgcctgagca aagttgatca
    13081 tcccgaaacc atcactccca agtgccctgg caagataggc gatcgccaaa aagttcagtc
    13141 cccgggcgac aacttgtcca aagaacaagg aaaaaaaatt ttttaggata gttgccctgt
    13201 cagccaatca aaactccatt cggttttcgt taaaagtggc taagtttctt ccgcttttt
//
LOCUS       DASWLN010000172        18245 bp    DNA     linear   ENV 20-FEB-2024
DEFINITION  MAG TPA_asm: Candidatus Zixiibacteriota bacterium isolate
            SMAG_U2949 k141_1118732, whole genome shotgun sequence.
ACCESSION   DASWLN010000172 DASWLN010000000
VERSION     DASWLN010000172.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37960594
            Sequence Read Archive: SRR10376091
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Candidatus Zixiibacteriota bacterium (soil metagenome)
  ORGANISM  Candidatus Zixiibacteriota bacterium
            Bacteria.
REFERENCE   1  (bases 1 to 18245)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 18245)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (26-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/12/2024 08:28:26
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 1,440
            CDSs (total)                      :: 1,416
            Genes (coding)                    :: 1,412
            CDSs (with protein)               :: 1,412
            Genes (RNA)                       :: 24
            rRNAs                             :: 1 (16S)
            partial rRNAs                     :: 1 (16S)
            tRNAs                             :: 21
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 4
            CDSs (without protein)            :: 4
            Pseudo Genes (ambiguous residues) :: 0 of 4
            Pseudo Genes (frameshifted)       :: 0 of 4
            Pseudo Genes (incomplete)         :: 3 of 4
            Pseudo Genes (internal stop)      :: 1 of 4
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..18245
                     /organism="Candidatus Zixiibacteriota bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_1118732"
                     /isolate="SMAG_U2949"
                     /isolation_source="soil"
                     /db_xref="taxon:2053527"
                     /environmental_sample
                     /country="USA: Oregon"
                     /lat_lon="42.99 N 123.63 W"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            complement(<1..2895)
                     /locus_tag="VGB16_06380"
     CDS             complement(<1..2895)
                     /locus_tag="VGB16_06380"
                     /inference="COORDINATES: protein motif:HMM:NF024180.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="tetratricopeptide repeat protein"
                     /protein_id="HEX9917348.1"
                     /translation="MKRGLGLFSLGFIFLGSVLCFSAQAQTWKELLDQADSLFRAASY
                     DSAIVVGKLALEKAKEEFGEEDTTVASVLNRIGACYLQKADYIEAENILKQALTIREK
                     VLGPEHPSVGKSLNNLANIYYDQAKYTEAEPLYKRALTIWEKTLGPEHPDVARVFHNL
                     ANIYSREGKYAEAESLYKRALAIREKAFGPEHPDVAANLGSLATLYGRQGKYAEIEPL
                     LERALVISEKALGPGHPDVATYLFNMGLLRKIQGKYAEAESLYKRALAIRERAFGPEH
                     PTVAMNLEVLATVYCWQAKYAEAEPLYRRALAINEKALGPEHPNVATTLGDLADLYCG
                     QARYAEAEPLYKRSLAIWEKVLGPDDPELAIDLRGLGGLYCDQGKYTEAEPLIKRALA
                     IDEEAFGPEHPAVAGDLAMLGYLYNSQGKYAEVEPLIKRALAMSEKVLGPDHPIVAGD
                     LENLAYLYRKEGKYAEAESLYKEVLSIIEKVFGPEHFNVTSGLVGLANLYRDQGRYAE
                     AESLYEQTLAMDEKTLTPYDPYMAYDLSDWALLLREAGRFTDSEAKEKRAYTIRRQNF
                     RDNSSVLSERDALTYSKFMRDEANQYISILLDSKDTSLDCQKEIASVVFCTKGQVSDG
                     IFARHKTEASFSDTSLKALADSLRYARFKLANLYVKGPDQEDVEGYKKKLQEASQNKE
                     RLEGELARRSVEFRKELELWDIDVTKASNSLPSNSILVEYLKYDRLKVKDQTEPHYLV
                     IVLDREGRVYIRDLGPSQGIDSLVASYRAHFQEISKRGGFVFSKDLEDYKAIATKLYE
                     KIWKPVQDKLGNSGLVFVAPDGELNLVSFAGLVDKDGKYLIEDYPLQYLASARDLVRL
                     IDKVQPNKGLFALGDPDFDASAQVRLSSTPLYAQTQDSTISLYTLRNIRSGCEELNSI
                     TVLPLPGTKRELENISSAWKKKTKEQALVFLGANASEENFKLNASGKKVIH"
     gene            3389..5161
                     /locus_tag="VGB16_06385"
     CDS             3389..5161
                     /locus_tag="VGB16_06385"
                     /inference="COORDINATES: protein motif:HMM:TIGR04514.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="GWxTD domain-containing protein"
                     /protein_id="HEX9917349.1"
                     /translation="MKKVLVLVILICLNSVAMAFALSLQHKDLLKVLKKAGASQAELD
                     TLVTLLKTNAPESQIQNRVNLFWSNKIKSLTDEEKVIYGGLADSTERIGYLSCKTKWD
                     RFWFLRKLNKIQKEKLWQTEKDRIFYFSFKLVLTNGEFKEYLNLPDSLKEAWLYTFWK
                     KKDPNPTTEENEFKVEFDRRVEYVLYNYWVPFGQLRPWDDRGDVYILYGESNEKEVPV
                     VYFDRGEYKFRPVGDVEFDSRGRSIPDRLSTEVWHYYGICSFQFEDRNKIGIWELNVY
                     RECDIQTPIEALSEFMKNTAEKVGLAKVEYIPDLGSPLDFPWNWWKFWNSGDYYDVRI
                     NLGIPIEKLGLMVDSSVSLDTGWLCFQERIVISDKDRKTISSDSLWIRKKVSKYLDRK
                     ELLLVDQYSCDSLVPGFYNIAISIKDSASNRMGIYQDTSVILVSHVKLKASEKISQLI
                     MADSVWEADSSYIERNGTKFIRNNLVIKPRPGNVYLSDQSPSFYCEIYELKEGEDDSC
                     RCKIYYYFLKKDDKTGNFDLFQGPFTEVASYPSKSQPYIKGSLSKDKFPSGEYIIRID
                     VYDLNDKITQPDQIRKTVVGFKKG"
     gene            complement(6745..10923)
                     /locus_tag="VGB16_06390"
     CDS             complement(6745..10923)
                     /locus_tag="VGB16_06390"
                     /inference="COORDINATES: protein motif:HMM:NF012298.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="tetratricopeptide repeat protein"
                     /protein_id="HEX9917350.1"
                     /translation="MEDIFSIRYKLLESLGKGGLGEVYHAFDTWEERDVAIKLANQET
                     LSKEEIENFKEEYRLLKQLKHPGLVEAYDFNYSGDCPYFSMEYVPGKSLEEAFREGGP
                     KLLNILMVELCEVLGFIHQRGIIHCDLKPDNIKVTSSPFRIKLLDFGLAEKKGSKINQ
                     EPKGTLFYMSPEVFQKKPLDERSDLYSLGVILYQATTLQLPFSYSDPVQVVSAHLEEE
                     PKPPRELNPQLPESLNDLILKLLAKDPKQRFGNATELKEFLKEKEKIDTSQRGFPSQD
                     TSQDPSQDPEFGEFGEFVLQHLFSGGLVNREEQLSRLWKGVLTARQSEGKFFLIEGEA
                     GVGKTELLDELKLKSQLEGIAFLKVRCLEKESETYVPIIEALTHIFPYLKTRCPLLIA
                     KYDQPLKLLIPQLKLDDEFVGAEFPSTLLRTVSLSNRTSAQKNGWRQAGSRRSPDLRD
                     FGQDESQVKEENVGVGFIENPPPNGGAIRPATSVSQECHCEDPAPEAGDEAISEKALK
                     TKRLPRSPDKSRSLAMTREGGEEQTADGDGLNEGVPQPGVSFSADDRDSLISGLADFL
                     IEASVVFPFALCFEDLLWADAGTLSFLKELSEHINESRIFLCASLRSEKLQEKKELKQ
                     LINSSAGKDWFEEIKLDRFDLKGTERFVISKLAGSHPPEEAVKYLFSHTSGNPLFLIE
                     ILKFLIQKGIIALRKGQWLFDLENLEKQEIPKQIGSILLDNLLRYDQKTKKFLGIASL
                     VGERFDLESTKILSELSDDQIFEILFVLLKDEVIHKITEQAELTYEFSSQSLQKLLYE
                     KFLTSQRRALHLKLGDLLEQKEKEGIPQPKEKLAFHYVNSSDYEKAHKYSLEAASETQ
                     KRFAHTQTLAHINNAIKSAQKLGDQNKFLDALMKRGDFYNSIGELDKALKDYKEILKK
                     AKGVKDERFMAQLYKNMSNAYRLKSNYKNTLVYSNKALDLFAELKDDLERANTLNIIG
                     GIYLVDSQYDEALKVFNETLKIKKAFDDQRSIAFTLVNIGTAHFFKNDCKRAEDYFGQ
                     YLDLVKKLGLKEEITAGLNNLSLVYYSLGEFNRGITYLLDSLELNKQIGNENEICYNY
                     ENLGQNFLECGDYETSLGYSEKGLNLAQQIGAPRSGDILLNIARAYLEIGDYEKSEQS
                     FQESLRFAKQISDKTLIASILLNWTKLRIILNDREKEKELLKDSNKIAKELKDDKLFL
                     ELYEIFGIMDLKEGKAEESIEYFKKAEEVVNKLKLKNRRLSLNLSFYENYSKLNQPKK
                     AKKYLEEARKLAEITQSKALKGEFYLKSAGNEMNNGNSDKSFSDLKKALEIAQAIGKP
                     ELIWRIHHLWGKLHIQNKDYEAGYKEFEKAFVIVKDIAQKIKNEKSRQSYMKDKKELM
                     EDLKKVAGVMVRVG"
     gene            complement(11230..14991)
                     /locus_tag="VGB16_06395"
     CDS             complement(11230..14991)
                     /locus_tag="VGB16_06395"
                     /inference="COORDINATES: protein motif:HMM:NF012298.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="tetratricopeptide repeat protein"
                     /protein_id="HEX9917351.1"
                     /translation="MDSGTFSGRYQILENLGIGGLGEVYKVIDLWTERELALKIATPE
                     ELAEDGGENFKTEFVLLKELVHPGIVEVYDFGYGEDGRPYFSMEYIKGKGVEEAFKEK
                     GYDFLYRFIWELCEILGYLHSRGIVHCDLKLDNLKVVEGLFGVKLLDFGLSEKIGSRV
                     GRQLKGTLDYMAPEIFSGGSLDGRTDLYSLGVILYQVTTSQLPFPSKDPVRVVAGHLE
                     GEVIPPRKLNPKIPLILDRLVLKLLKKSPDKRYQSVNQLKRAIEERTRLKTDITRPEL
                     LYNHIYSGGIVGREKELKEIDSILKRAVGLKGQMVLLEGELGVGKSVLLKQVKLKSQL
                     EGTLFLEGRCFENESNPYQPWVGIASNLAKYLDRNFPFFTERFRDLLNFLEDGTKNKE
                     GVSSISGAPHIEQRNRFFNELVTFLVEGSAVSPFALSIDDLHLAGSGTLKLVELLSKR
                     IERSRIFFCICFRTEALRTNSKLKILINRFGENSAFQQIQLGRLSFEQTKEYFATKFF
                     GTSLPKELISYAYYYTSGNPFFLTELTKYLVKTRIITSRDSKWILDLKNLDKLDIPRK
                     VETVIWDNIRRYDNNVIRFLSVASIVGERFNLRSIEYLSGYDEEAILLVLFVLLKDQV
                     LRKTVGKFRGETCYEFSSRSLERLLYDRLGRRRKAELHHRMAVLLESDYSRGQEELVE
                     EIAYHYSRTRDYLKAYNFSLVAAKKMRGKFANRQALVYLNNALRFSKMIKDKKESEYR
                     KLEALAERGDLHRARGKLGRALKDSTAALKIAKARKDIRWVAHLCKSIGMVYEQKKDF
                     KRGIVCLKEALRIYRSLKNPQEEADTLKRVGAIYRMKLEHRKALHNYRRALRIMNAIR
                     DIEEMTGILNNIGVIYLDQGKYGKCLDHFKQCLSIAKRLGEDKVMTKALNNLGVAYTD
                     LGKYNLAENHYLEALKLDRKIGDERSIYYRLHNLGDVAQKKAEFTKALDYYVQTLGLT
                     KKNDLPIGGELRSIGVVNLEIGKYSEGKEYLEKALEEAERTKDRALQAEILLNLSKLH
                     SLLKQDGYSEQYLLRSTKLWKKIEDKGLLASFYCDAALVKLKNSHPEQGLSFLDRAQK
                     LTSHLGIKEREVPIKMAYCELYDKLDKKEKLDYLLKELRKLMKKREEQLYEPEYHLFS
                     GKREWEKGHISKSLEHLETALDRAEKMGKPELLWRIHHLLGKFNLALNNVEKSYFFFD
                     KAREKLVWLASHIKDVSLRESYLEDEDKEGLLDDMRLLEKTATHTAAAV"
     gene            complement(14995..>18245)
                     /locus_tag="VGB16_06400"
     CDS             complement(14995..>18245)
                     /locus_tag="VGB16_06400"
                     /inference="COORDINATES: protein motif:HMM:NF037660.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=3
                     /transl_table=11
                     /product="tetratricopeptide repeat protein"
                     /protein_id="HEX9917352.1"
                     /translation="EIFSGGSLDGRTDLYSLGVILYQVTTSQLPFPSKDPVRVVAGHL
                     GEEPTPPRDLNPTIPYELDDLILDLLDKLPEGRPRNALEVKERISKLSQRNLGTKEDG
                     LYSSYIYSGELIGREKELARLEQSARESIHAQGKVVLLQGEAGIGKSTLLRRLKMSVQ
                     FEGIPFLMAGCSHKDLAPYQPIRQILYEFFPYLKNKHDFLLQKYSHPLSLLVPELLSP
                     VKESVDLGKKGREDFCREIVDFLIEASLVVPLTICIDDLDMCGEESFVVLENLANSIH
                     RSKIFLCCSFPIGCKRKDIGHLLKKIKCSCGEGAHLELNPFSAKDIKEFISSRLLRAP
                     SGEVTSLLHDHTKGSPFALTEWLKFLLENKVIQLRPDNAKYDLESLKEIKPPCSEKDI
                     ILKNLDRHGREALELLHLCILSGGGANMEQLSLLAGRSKEELEYPLFILSEDHLMRRA
                     VKSGERGFCYRLVHPFIERFLPDVFSHQDRKALHQRIALYLDKSSSRESYPPIENMAY
                     HYFQGGDHQKGFEYCIKTADKMQRLYAFEKTLYWLENAQRLAKKFTGRKERLKREQLL
                     AMKRGDSYRDMKENDKSLENYRKALKLSQSLQDKERIAEVYSKISVIYESQNNIPKAL
                     FCLRKGLKIYQSLNNKLKMAEIYESVAYLVCIKEFQYGKSLEYYRKSLRLYRLLSDHK
                     GEARCLNKLGVTYTYQGEFSKSKECLKQFLEIAEKIGDKEMCSQGLNNLAIICFCTDK
                     YSEGIEYLLAALRINEEIDRKEGILFNLANLGDKFCRVGKFKQAISYSLRCLDLARQV
                     NSPKFRKGTALLSLGLSNFELGNYDQAIRYLEESLESAEQADDKHLRLKISMELAGFY
                     LNMNQLEKTEELLASAEEILKDVQDKHDLIFFYEKMGWLNLKKEKSEQAEEYLNKAKQ
                     LVQQVGIRDENILVNLLLCDAYFESGELKKVSQTLSQTQSLLGKSENKRYEPQYYLNL
                     AKKEWWDGRLDQALRYLEISLTKAEKQETPETIWRIHHQLGKLSLSMNNLERAYKEFR
                     AAQNIIKELSTKVGNENMKKRYFEDKEKIELLSDIRSVVELMMGEKVVHSLG"
BASE COUNT         4751 a         4322 c         3561 g         5611 t
ORIGIN      
        1 gtggataacc ttctttccag aagcgtttag cttgaagttc tcctcagagg cattggctcc
       61 cagaaagact aaggcttgtt ccttggtctt tttcttccag gcagaggata tattctctag
      121 ctctcttttg gttccaggta aaggtaggac tgtgatacta ttcagctcct cacatccaga
      181 gcggatgtta cgcagagtgt agagagagat agtagagtct tgggtttggg cgtaaagggg
      241 ggtggaggaa agacggactt gagcagaagc gtcaaagtcc gggtcgccta aggcaaaaag
      301 gcctttgtta ggctgaactt tatctattag tcttaccagg tctcgggctg aagccagata
      361 ctgcaaagga tagtcttcaa tcaggtactt accatctttg tctaccagac cagcgaagga
      421 gaccaagtta agctctccat ctggggccac aaagacaagg ccactgttgc ctaatttatc
      481 ctgaactggc ttccagatct tttcatatag ttttgtggct atagctttgt aatcctccaa
      541 gtctttggag aaaacaaatc ctccccgctt agagatctcc tgaaaatggg ctcgataaga
      601 agctaccaag gaatcgatcc cttgagaggg cccaaggtct ctgatgtaaa ccctgccttc
      661 tctgtctagg acaataacca gataatgagg ttcggtttgg tcttttactt tcaggcggtc
      721 atacttgagg tattcaacca gaatagagtt agagggaaga gagttggatg ccttagttac
      781 atctatatcc catagttcaa gctcttttct gaactccaca ctccttcggg ctaactcccc
      841 ttccagacgt tccttatttt ggcttgcctc ttgtagcttt ttcttgtaac cttccacatc
      901 ctcctggtcc ggacctttga catagagatt ggccaactta aaacgagcat atcttaaaga
      961 atcagccagg gcttttaagc tggtgtcaga aaaggaagct tcggtcttgt gtctggcgaa
     1021 gatgccatct gagacttgac ctttggtaca gaagactaca ctagctatct ccttctgaca
     1081 atctaaagat gtatccttag agtcaagtag tatggagata tactgattgg cttcatctcg
     1141 catgaactta gagtaagtta gagcatctct ttccgagagg actgaggagt tgtcacggaa
     1201 gttctggcga cggatagtgt aggctctttt ttcttttgcc tctgaatcag tgaaccgacc
     1261 tgcctctctt aacaataaag cccaatcact caaatcgtat gccatataag gatcgtaagg
     1321 agtcaaagtt ttctcatcca tagctaatgt ctgttcgtaa agtgactcag cttccgcata
     1381 tctaccctga tcacggtaga gatttgccag tccgactaaa ccagacgtta cattaaagtg
     1441 ctcaggtcca aaaaccttct ctataattga tagtacttct ttgtaaagag attcggcttc
     1501 agcgtatttc ccttcttttc tataaagata cgcaagattt tccagatccc cagccacaat
     1561 agggtggtca ggacccagga ctttctcgct catagccaag gctcttttga tgagaggctc
     1621 gacttcagca tatttgccct ggctattata gagatatccc agcatagcca ggtctcctgc
     1681 tacggcggga tgttcaggac caaaagcttc ctcgtctatc gccagagctc gcttaataag
     1741 aggttctgct tctgtatatt tgccttgatc acagtagaga cctcctagac ctcttaagtc
     1801 tatcgctaac tctggatcat cgggacccaa gactttctcc caaatagcca gagaccgttt
     1861 atatagaggt tcagcctcag catatctagc ttgaccgcag tagagatccg ctagatcgcc
     1921 aagggttgtt gctacattag gatgctcagg gcccagagct ttctcgttta tagccagtgc
     1981 acgcctatat agaggttcag cctcggcata tttggcttgc caacaataga cagttgctaa
     2041 aacctccagg ttcatcgcta cggtgggatg ctcaggacca aaagctctct cccttatagc
     2101 caaagctcgt ttatatagag actctgcttc ggcgtatttg ccttgaattt tgcgcagaag
     2161 gcccatatta aacaggtatg ttgctacgtc cggatgacca ggacccaagg ctttctcact
     2221 tataacgagt gctcgttcta acaggggttc aatctcggca tatttgcctt gacggccgta
     2281 aagagttgct agacttccta agtttgccgc tacgtcggga tgctcaggac caaaagcttt
     2341 ctcccttatg gccaaagctc gcttatatag agactctgct tccgcgtact tgccttcgcg
     2401 ggaatatatg ttggctagat tatggaaaac tcttgctaca tccggatgct ctggacccaa
     2461 ggttttctcc caaatagtca aagctcgctt gtatagaggt tcagcctcgg tatatttagc
     2521 ttggtcgtag tagatattcg caagattgtt taggcttttt cctacacttg ggtgttcagg
     2581 tccgagtacc ttctctctta tagtaagcgc ttgctttaag atgttctcag cttctatgta
     2641 gtctgccttt tgcaggtagc atgcgccaat tctattcagc actgaagcga cggtggtatc
     2701 ctcctctcca aattcttctt tggctttttc taaagccagc tttcccacca caatagcaga
     2761 atcataacta gctgccctga aaagcgaatc tgcctgatca agcagctctt tccaggtctg
     2821 agcctgggca gaaaagcaca acactgatcc taaaaagata aatccaagcg aaaacaagcc
     2881 aagtcccctt ttcatcttat cctccatcaa tattttcact tattgcacca gattaactaa
     2941 taagccaatg aggttctatt gtcaaacaaa aaatctaaga aaacataaaa ttggcaaatt
     3001 tctttaaaga tttttgggaa ccttaagagg gttcatacga ctaatagggt gagatgaaac
     3061 aaaaccacaa aaaactcgta aaggaggtga gaaaagatga agaagaagat cctagtggtg
     3121 cttctaatag tatttgcttt gacatctgtg gttcagttgg ctatgattag tcatccccag
     3181 acggcagatg cgatgggact accatcgaag tgttgcacag aatgttactg ggcgtgtact
     3241 aatgatcata tctgtacccg ttactgtttc acatattgtg gagctactgt gtgtgatcat
     3301 cttgaccaga atagttatta ttgatccaat acaataacca ttctggatag ttataagaca
     3361 agatgtaaac atccaaaagg tgtagagcat gaaaaaagtg ttggttttag taatcttaat
     3421 ttgtttgaat tctgttgcga tggcttttgc attaagcttg cagcataaag accttctaaa
     3481 agtattgaag aaggctggtg cctcacaagc agaactcgat acactggtga ctttacttaa
     3541 gaccaatgct ccagaaagcc agattcaaaa tagagtaaat ctgttttgga gcaataagat
     3601 taaatccctt accgatgaag agaaggttat ttatggaggt ttagcagatt ccacggaaag
     3661 gattggttat ctatcctgta aaactaaatg ggacagattc tggtttctaa gaaagcttaa
     3721 taaaattcag aaagagaaac tctggcagac tgagaaagat aggatattct atttctcttt
     3781 taagttggta ttaacaaatg gagaattcaa agaatatctt aatttaccag attccttaaa
     3841 ggaagcatgg ctctacacct tttggaagaa aaaagaccca aatcccacta ctgaagagaa
     3901 tgagttcaag gttgagtttg atagaagagt ggaatatgtt ctgtataact attgggttcc
     3961 ttttggacaa ttacgacctt gggatgatag aggagatgtg tatatcttat atggggagtc
     4021 aaatgagaag gaagtaccag ttgtttattt tgaccgaggt gaatacaaat tcagaccagt
     4081 gggtgatgtt gaattcgata gcagagggcg atccatccca gaccgtcttt ccacagaagt
     4141 ctggcactat tacggaattt gttctttcca atttgaggat aggaataaga taggtatttg
     4201 ggagttaaat gtttatagag aatgtgatat ccaaacaccc atcgaagcat tatctgaatt
     4261 tatgaagaat acagccgaga aagttggact cgctaaggtc gaatacatcc ccgacctcgg
     4321 ctctccttta gacttcccct ggaactggtg gaagttctgg aatagtgggg attattatga
     4381 tgtgaggata aatcttggta ttcccattga gaagttagga cttatggttg attctagtgt
     4441 ttctttagat actggctggc tttgtttcca ggagaggata gtgatatcag acaaagacag
     4501 gaaaacgatt tcttctgatt ctttgtggat tagaaagaaa gtatccaagt atctggatag
     4561 aaaggagtta ctcttagttg accaatacag ctgtgatagt cttgttcctg gattctataa
     4621 cattgccata agcataaagg atagcgcttc taacagaatg ggaatctatc aggatacctc
     4681 ggttatattg gtatctcatg ttaagcttaa agccagtgag aagataagcc agcttattat
     4741 ggctgattct gtgtgggaag cagattcctc ttacatagaa agaaatggaa ctaagtttat
     4801 caggaacaat ctggtgatta aaccccgacc tgggaatgtg tatctttcag atcaatctcc
     4861 cagcttctac tgtgagatct atgaacttaa agaaggagaa gatgacagct gcaggtgtaa
     4921 gatctactat tacttcttaa agaaagatga caaaacagga aactttgacc tctttcaagg
     4981 acctttcaca gaagttgcat cctacccctc caagtcccaa ccctatatca aaggaagctt
     5041 atccaaagat aagttccctt ctggtgaata catcataaga atcgatgtgt atgacctaaa
     5101 tgacaaaatc acccaaccag accagataag aaaaacggtg gtggggttta agaaggggtg
     5161 aggaaaagaa aatgtagggg tcgcatttat gcgacccggc gggcttaata aatccctggc
     5221 cgggcaggca agcccctaca aatacaaaac tatattgtgc ctgccaagct cccccgactc
     5281 ggcagaaggc gacattccgg aatgtcgcct gcgttattgt aggggtcgca tttatgcgac
     5341 ccggcggact cttttaaccc ctcctaacct ccccttatca ggggaggaac attctatgtc
     5401 tacctcaagg ccgtgtcata atgtcattct gaggcgttag ccgaagaatc tcgattttgt
     5461 aacttattga aactattaga ttcttcgctt tgctcagaat gacaattgtg tgtctttaac
     5521 gtacgacaag tccccctgat aagggggata aagggggtta ggttcaggaa aaaccaacct
     5581 aaaacaataa aaactggatt cccgccttcg cgggaatgac agacggaatt ctatatcttg
     5641 tggtaatcta aactaattca acaagatatg gaattatggt gaattttctt acaaaaaacc
     5701 aagaaaaccc ttgacaaagt acccttgcat tttgcaaatt acacacagaa gaattatgtc
     5761 ttcgtctaga agcattggta agcatcagct tatcttcacg ttttattcgt attacaaagc
     5821 gttttaaaac agaatagaaa acaaaaaacc ctaaggggat tctatatctt tcaaattccc
     5881 ttgggaaaca caggaggcgc tatggaactg aaagacctga agttggaggt cgaggagctc
     5941 gaggaaaaga tcgcaccatt agccgcttct atcaaaggcg gattagcgta agactcgacc
     6001 gcacctcctt ttcaggggtg caccttaacg ggtgcacccc tttttatttt tgtaggggtc
     6061 ggattcatcc cccctttggc gggattcatc cgacccgcgt tatttgaagg cgacattccc
     6121 tggccgggca ggccggaatg tcgcctgcgt tatacagggc tattctgtca ggtcagcccg
     6181 cccgtttctc cctactcgac ccaacggcca gggggaatcc gacagacaca ataatccgca
     6241 cagggatgaa ttccctgtgc tacgaatata acatatcgta ggtgtgggaa tttatcccac
     6301 accgattagt tgatttgaag gcgaacccgt acgggtcgcc tgcgttattg taggggtcaa
     6361 tattttgcca cccgcccgga ctctcttaac ccctcctgac ctccccttat caggggagga
     6421 acgtttcgtg tccacctcaa ggccatgtca taatgtcatt ctgaggcgta gccgaagaat
     6481 ctctcttgcc tcccattccg cccagattct tcactccgct agcgctccat tcagacttgt
     6541 cctgagcgaa gccgaaggaa tgacagaagc ggagcctgtc ctaaagtcga aacgaatggg
     6601 ttcagaatga caattgtctc tttagtgcgt gacaagtccc cctgataagg gggataaagg
     6661 gggttctttt tatacgactc ggctcattcg caggcgacat tcgcgaatgt cgccttcaaa
     6721 ataccccgta cattaatcat tgttctaccc caccctcacc ataaccccag ctactttctt
     6781 taaatcctcc atcaactctt tcttgtcttt catataactc tgccttgatt tttcattttt
     6841 tattttctgc gcaatatctt taacaatcac aaaagctttt tcaaactcct tataccccgc
     6901 ctcataatct ttgttttgga tatgtaattt cccccaaaga tgatgaattc tccagataag
     6961 ttctggtttt cctatcgctt gggcaatttc taacgctttt tttaaatccg aaaagctttt
     7021 gtctgaatta ccattattca tttcgtttcc agcagacttc aaataaaact ctccctttag
     7081 ggctttgctt tgagtgattt cagccagctt gcgagcttct tctaaatact tttttgcctt
     7141 tttgggttga ttcaatttag aatagttttc atagaagctc aaattcaaag atagtctcct
     7201 attctttaat ttcaatttat taacaacttc ttctgccttc ttgaaatatt cgatactttc
     7261 ttctgcttta ccttctttca aatccataat cccgaagatc tcataaagct ccaaaaagag
     7321 cttatcatcc tttagctctt ttgcaatttt gttggaatct tttaacaatt ctttttcttt
     7381 ttccctatca ttcaatatta tgcgaagctt ggtccaattc aagagaattg aagcaatcag
     7441 agtcttgtca ctaatctgct ttgcaaatct caatgattcc tgaaagcttt gttcagattt
     7501 ttcatagtcc ccaatctcta agtaagctct ggcaatgtta agcaaaatat cccctgaacg
     7561 aggcgctcct atttgttggg ctaaattcaa tcccttttcg ctgtagccta aagatgtttc
     7621 ataatcccca cattctaaaa agttctgtcc cagattttcg tagttataac atatctcgtt
     7681 ctcattccct atctgcttgt ttagttccaa agagtccaga aggtaagtaa tgcctctgtt
     7741 gaattctcct agcgaataat agacaaggct taaattgttt aaccctgcag taatctcttc
     7801 tttcaggcct aactttttga ctaagtcaag atactgccca aaataatctt ctgctctctt
     7861 gcagtcgttt ttgaagaaat gagctgtccc gatattgacc aaagtaaaag caatactcct
     7921 ttggtcatca aatgcctttt ttattttcaa tgtctcattg aataccttca acgcctcatc
     7981 atattgtgaa tcgacaagat aaatgccacc aataatgttt agagtgttag ctctttctaa
     8041 atcatccttc aattctgcga aaaggtccaa tgccttgttt gagtaaacta aggtgttttt
     8101 gtaattggac ttcagtctat acgcattact catattcttg tatagctgtg ccataaacct
     8161 ttcatccttc actcccttag cttttttcag aatttcttta taatccttta gcgctttgtc
     8221 caattcccca attgaattat aaaaatcccc ccttttcatc aaagcatcca ggaatttgtt
     8281 ttggtcgcct aacttctgtg cagattttat ggcgttgttt atatgtgcta aggtctgggt
     8341 gtgggcgaat cttttctggg tttcgcttgc ggcttctaag gagtatttat gggctttttc
     8401 gtagtccgaa gagtttacat aatgaaaagc gagtttttcc tttggctgtg ggattccctc
     8461 tttttccttt tgctccaaaa gatcgcctaa tttcaaatga agcgctcttc tttggcttgt
     8521 aaggaatttt tcataaagca atttctgtaa gctctggctg gaaaactcat aagtcaattc
     8581 tgcctgttcg gttatcttgt ggatgacttc gtcttttaag agcacgaaaa gtatctcaaa
     8641 aatctggtca tccgaaagct cggaaaggat tttcgtgctc tccaaatcaa atctctctcc
     8701 gaccaaactg gcaattccca gaaatttctt tgtcttctga tcatagcgga ggaggttatc
     8761 caaaaggatg cttccgattt gcttgggaat ttcttgcttc tccagattct ctaaatcgaa
     8821 gagccactgg ccttttctta aagctattat ccccttttgt ataagaaatt ttagaatctc
     8881 aatcaaaaac aaaggattac ccgaagtatg gctgaaaagg tatttaactg cctcttccgg
     8941 ggggtggctg ccggctaatt tcgaaattac aaagcgttcc gtccctttga ggtcaaatct
     9001 atccagtttg atttcctcaa accagtcctt gccagctgaa ctgtttatca gctgctttag
     9061 ctcttttttc tcctggagtt tttcagaccg gagtgaagcg cacaggaata tcctgctctc
     9121 gtttatgtgt tcgcttagtt cttttaggaa actcaaagtg ccggcatcag cccacagcaa
     9181 atcctcaaaa caaagggcaa agggaaaaac aactgacgct tcgattagga aatcagctaa
     9241 gccggaaatc aagctgtccc ggtcatccgc cgaaaagctc acccccggct gcggaacccc
     9301 ttcgttcaac ccatccccgt ctgcagtctg ttcctcgccc ccttctcttg tcattgcgag
     9361 cgacctcgat ttatcagggg agcgtggcaa tctctttgtt ttcagtgcct tctctgagat
     9421 tgcttcgtct cccgcctctg gggcgggatc ctcgcaatga cattcttgag acacgctcgt
     9481 ggcgggtcgg atcgccccgc cgtttggcgg gggattttca ataaatccga cccctacatt
     9541 ttcttccttc acctgagatt catcctgtcc gaaatctcgc aggtcaggag acctgcgact
     9601 acccgcttgt ctccacccgt ttttttgggc ggaggtacgg ttcgacaggc tcaccgtcct
     9661 gagcaaagtc gaaggaaact ccgcccctac gaattcatca tctaacttca gctgcgggat
     9721 caaaagcttc aagggctggt catatttggc gatcagaagc ggacatctgg tttttaagta
     9781 tgggaaaata tgggtaaggg cctcaattat cggaacataa gtctcgctct ccttttccaa
     9841 gcatctgact ttgagaaagg caatcccttc caattgagat ttcagcttca actcatccag
     9901 aagttcggtc tttccaactc cggcctcccc ttcaattaaa aagaactttc cctcgctctg
     9961 gcgagcagtt agaactccct tccacaatct ggatagctgc tcttctctat taaccaagcc
    10021 tccgctaaaa agatgctgta acacgaattc cccgaattcc ccgaattctg ggtcttgaga
    10081 tgggtcttga gatgtgtctt gagatgggaa gcccctttga ctggtatcta ttttctcttt
    10141 ttcttttaaa aactctttta actctgtagc gttcccaaac ctttgctttg ggtctttggc
    10201 taaaagtttc aatattaaat catttaagct ttccggaagt tggggattca attctctggg
    10261 cggttttggc tcttcttcca gatgggctga aaccacctgc accggatcag aataagaaaa
    10321 aggtaactgc aaggtggttg cctgatagag tatcactccc agggaataca ggtcggaacg
    10381 ttcatccagc ggtttcttct gaaaaacttc gggcgacata tagaacaaag ttcccttggg
    10441 ctcttggttt attttggagc ctttcttttc agctaagccg aagtctaaga gctttatcct
    10501 gaaaggcgag gaggtgactt tgatgttatc cggtttcagg tcgcaatgaa ttatgcctct
    10561 ttgatgaata aatcctaaaa cctcacacag ttctaccatc aagatgttca acagcttcgg
    10621 tccaccttcc ctgaaggctt cttctaagct ctttcctggt acgtactcca tagagaagta
    10681 agggcaatcg ccggagtaat tgaaatcata tgcttccacc aatccaggat gcttaagctg
    10741 ttttagaagt ctatattctt ctttgaaatt ctctatttct tcttttgaca acgtttcctg
    10801 gttagctaac tttatggcta catctcgctc ctcccaggtg tcaaatgcat gatagacctc
    10861 gcccagccca ccttttccca gactctctag taatttgtaa cgaatagaga aaatatcttc
    10921 catattattt tatcatagta cccacgctct acatgcgtgg tccttgttgt ttgtaggggc
    10981 aattccccgc ttttaacggg atgtgccaca tgaattgccc ctactatgtt ttgtcatccc
    11041 cgagggttta atcggggatc catacttgct ttccttttct ggatttcccg tatccagtac
    11101 gggacaagct ctgcgcgaat tctgcgtctt gagactggag cttatcccgc agaaatgcgg
    11161 gacaggaatg acataaaaac agtgaactgc tctcactctt gccttaacag ctggggagtt
    11221 cccgctggct taaactgcgg cggcggtgtg agttgcagtt ttttccaaga gtctcatgtc
    11281 atccagaagc ccttctttgt cctcatcttc aagataactt tccctaagtg acacatcttt
    11341 tatatgactg gccaaccaaa ccaacttctc tcttgccttg tcaaaaaaga aataggattt
    11401 ctccacgttg ttcaaagcga gattgaattt acccaaaaga tgatgaatcc tccaaagtag
    11461 ttccggtttt cccatcttct cggctctgtc caaagctgtc tctaaatgct ccaaagattt
    11521 gcttatatgc cccttttccc attctctttt gcctgaaaat agatgatact ctggttcgta
    11581 aagctgttcc tcccttttct tcatcaattt ccttagctct tttagtaaat aatccaattt
    11641 ttcttttttg tccaatttat catacaattc gcaataagcc atctttatgg gcacttccct
    11701 ttcctttatt cccaggtggc tggttaattt ttgtgctctg tccaggaaac tcaatccttg
    11761 ctcaggatga ctatttttca attttactaa agcggcatca cagtaaaaag aagccaaaag
    11821 acccttgtct tctatcttct tccatagctt agtgcttctg agtagatact gctcgctgta
    11881 tccatcttgt ttcaacagac tatgtagctt gcttaagttt agcaagattt ctgcctgcaa
    11941 agccctgtct ttagtccgct cagcttcttc cagcgccttt tccaaatatt cctttccttc
    12001 agaatacttt cctatttcta agttaactac tccgatgctt cggagttcac caccaattgg
    12061 caggtcgttt ttcttggtta agcccagagt ttgaacataa tagtccaacg ctttagtgaa
    12121 ttccgctttt ttctgggcta cgtccccaag attatgcagg cgataataga ttgacctttc
    12181 atctccgatt tttctatcta atttcaatgc ctcaagatag tggttttctg ctaagttgta
    12241 cttgcccaaa tcagtataag ctacgcctaa gttattcagc gctttagtca tgaccttgtc
    12301 ttcccctaat cttttagcga tagacaaaca ttgtttgaaa tgatctaaac acttcccata
    12361 tttcccctga tctaagtata taactcctat attgttaagg atacctgtca tctcctcgat
    12421 atcacgaata gcgttcatta tacgcaatgc cctcctataa ttatggaggg cctttcgatg
    12481 ttcaagtttc atacgataga tagcaccaac tcttttcaaa gtatccgcct cttcctgggg
    12541 attcttcaaa ctccgatata ttctcaacgc ctctttgaga caaactattc cccgtttgaa
    12601 gtccttcttt tgctcataga ccataccgat gctcttgcac agatgagcca cccatctaat
    12661 gtctttccgc gccttggcta tcttgagggc agcggttgaa tctttcagag ccctgcccaa
    12721 cttccctcta gctctatgta gatccccccg ctctgcaaga gcttcaagct ttctatactc
    12781 gctttccttt ttatccttga tcatcttgct gaatcgcaag gcattattta gatacactaa
    12841 agcctgacga ttggcaaact tccctcgcat tttcttggca gccaccagag aaaaattgta
    12901 agctttcaag taatctcggg tgcggctgta gtgataggct atctcttcga ctagctcctc
    12961 ctgacctcta ctgtaatcac tctctaagag gaccgccatt ctatgatgca attccgcctt
    13021 tcttctccta ccaagtctat catagagtaa tctctcgaga cttctactag agaattcgta
    13081 gcacgtttcc cctctaaatt ttcccactgt cttacgcaat acctgatcct ttagaagaac
    13141 aaacaggaca agaaggatcg cctcctcgtc atacccgctc aaatactcaa tcgatcttaa
    13201 gttaaacctc tcacctacaa ttgaagccac actaaggaat ctaatgacat tattgtcata
    13261 tctccttatg ttgtcccaaa tgactgtctc cactttcctg ggaatatcca atttgtccaa
    13321 gttttttaga tcgagaatcc acttactatc ccgggaagta ataatcctgg ttttcaccaa
    13381 atacttagtc agctcagtca aaaagaaggg gttgcctgag gtataataat aagcatagga
    13441 aattagttcc ttgggcaaac ttgtaccaaa aaacttagtc gcgaagtatt ctttagtttg
    13501 ctcaaaagaa agcctcccaa gttgaatttg ttggaaagcc gaattctctc cgaacctatt
    13561 gatcagtatc ttgagtttac tgtttgttct aagcgcctcc gtcctgaaac agatacaaaa
    13621 gaatattcta cttctctcga ttcttttgct caaaagctcc accaacttca gagtcccact
    13681 ccctgctaaa tgcagatcat caatcgagag ggcaaagggg gaaacagctg aaccctccac
    13741 caagaaagta accagctcgt tgaagaacct atttctttgt tcaatgtgag gggctccaga
    13801 aatggacgaa accccctctt tgttctttgt tccatcttca agaaaattca aaagatccct
    13861 gaatctctct gtgaaaaaag ggaagttcct gtctaagtat ttggctaaat tggaggctat
    13921 ccctacccag ggttggtagg gattggattc attttcaaaa cacctaccct ctaagaaaag
    13981 agtaccctcc agctgacttt tcagtttaac ttgcttcaag aggacgcttt tccccacacc
    14041 caactcccct tccaatagaa ccatctgccc ttttagccct actgctcgct tgaggatact
    14101 atctatctct ttgagctctt tttctcttcc tacaattccg ccgctgtaaa tatgattata
    14161 caatagttcg ggtcgggtta tatctgtctt cagtctggtt ctctcttcta tggccctctt
    14221 aagctgattc acactttgat accgtttatc cggtgatttc ttcaatagtt taaggacaag
    14281 gcgatcgagg attagaggaa ttttaggatt caacttgcga ggaggaatga cttccccttc
    14341 caagtgcccc gccaccaccc taactggatc tttggagggg aaaggtagtt gtgaggtggt
    14401 cacctgatag aggataactc ccagggagta gaggtcagtt cggccatcta agcttccacc
    14461 gctgaagatc tctggagcca tgtagtctaa ggtcccttta agctgcctgc ctacccgact
    14521 tcctatcttt tcggatagtc cgaaatccaa cagtttgacg ccaaataggc cctccaccac
    14581 cttaagatta tccagcttca ggtcgcagtg aactataccc cgagagtgca gataacctaa
    14641 aatctcgcat agttcccaga tgaaacgata gagaaaatca tatcctttct ccttgaaggc
    14701 ttcctccacc cccttgccct taatgtactc catagagaaa taagggcgac cgtcctcgcc
    14761 atacccaaaa tcgtatactt ccactatacc tggatgaact aattccttaa ggagcacaaa
    14821 ttcggtcttg aaattttctc ccccatcttc agccaactcc tccggggtag ctatcttcaa
    14881 ggccagctct ctctctgtcc ataggtcaat aaccttatag acttccccta atccccctat
    14941 tcccaaattc tctaatatct gatagcgacc agagaacgtc cccgaatcca tatctcatcc
    15001 taagctgtga actactttct cccccatcat caactctacc acacttctta tatcagaaag
    15061 cagttcaatt ttctctttat cctcgaaata cctcttcttc atattttcgt tccctacttt
    15121 cgtgctcaat tcctttatga tgttctgcgc ggccctaaac tccttgtaag cgcgttccag
    15181 attattcata gaaagactta acttacccag ttgatggtga atcctccaaa tcgtttcagg
    15241 ggtttcctgt ttttcggctt tggtcaggga aatttccaaa tatctcaggg cttggtccag
    15301 tctgccatcc caccattcct tctttgccag atttaagtaa tattgcggtt cgtatctctt
    15361 gttttcactc tttcccaaaa gagattgggt ctggcttaag gtctggctca cttttttcag
    15421 ctctcccgac tcaaagtaag catcacaaag aagaaggttc acaagaatgt tctcatctct
    15481 tatacctact tgttgaacca gctgcttagc tttattcaga tattcttctg cctgttccga
    15541 tttctctttc ttgagattta accaacccat cttttcgtag aagaaaatca ggtcgtgctt
    15601 gtcctgaaca tcttttaata tttcttcagc cgaggcgagc aactcttccg tcttctccag
    15661 ttggttcata tttaaataaa atccagccaa ttccatggat atctttaacc tcagatgttt
    15721 gtcatcggct tgctctgcgc tctccaaaga ttcctctaga tatcttatgg cttgatcata
    15781 gtttcccaat tcaaaattgc tcagccctaa actcaagagc gctgttcctt ttctgaattt
    15841 aggtgagttt acctgacggg ctaagtccag gcaccttaag ctgtaactta ttgcctgctt
    15901 gaatttccca acccgacaaa atttatcccc cagatttgcc agattgaaaa gtatcccttc
    15961 ctttctatct atctcctcgt ttattcttaa ggcggctaac agatattcta tgccttcgct
    16021 atatttgtca gtgcagaaac agatgatagc cagattgttc aatccttggg agcacatctc
    16081 cttatcacca atcttctcag ctatttctaa gaattgcttc agacattcct ttgatttaga
    16141 gaattcgcct tggtaggtat aagtcactcc cagtttgttc agacatctgg cttccccctt
    16201 atggtcgctc agcaggcgat acagccttaa agattttcta tagtattcga gtgactttcc
    16261 atattgaaac tcctttatgc aaaccaaata tgcaaccgat tcatagatct ctgccatctt
    16321 gagtttgtta ttcaggctct gatatatctt tagacctttt cttaagcaaa atagtgcttt
    16381 agggatgttg ttctgtgact cataaattac gctgatcttc gaataaactt ccgctatcct
    16441 ctccttgtct tgtaaggatt gggaaagctt taaagccttc cggtaatttt ccagggactt
    16501 gtcattttct ttcatatccc tgtaagaatc cccgcgcttc atcgccaaga gttgctctct
    16561 tttcaatctt tcttttctgc cggtgaattt cttcgccaat ctctgggcgt tctctaacca
    16621 atagagcgtt ttctcaaaag cgtaaagcct ctgcatctta tcagcggttt ttatgcaata
    16681 ctcgaagcct ttttgatggt ccccgccttg aaagtaatga taagccatat tctctattgg
    16741 agggtaggat tcccttgaac tgcttttatc cagatacaaa gctattcttt gatgtagggc
    16801 ttttctgtct tggtgactga agacgtccgg cagaaagcgc tcaatgaatg gatgaaccaa
    16861 cctgtagcaa aaacccctct cgccagattt gaccgccctt ctcatcaagt gatcttctga
    16921 cagaataaag agagggtatt ctagttcctc tttggatcgt ccggccaaca gagacaactg
    16981 ttccatattc gcccccccac ctgagagtat gcacagatgc aagagctcta atgcctccct
    17041 gccatgtcta tccagattct taaggatgat atccttctcg gaacaaggtg gtttgatttc
    17101 ctttaagctc tccaggtcat atttggcgtt atcaggtctc aattggatga ccttgttttc
    17161 cagcaaaaat ttaagccatt ctgtcagggc aaatggactc cccttggtat ggtcatgaag
    17221 cagggaggtc acctctccgc tcggtgctct caagagtctt gaacttatga attcctttat
    17281 atctttggct gagaatggat tgagctcgag gtgtgctcct tctccacaag aacacttgat
    17341 cttctttaac aggtgaccta tatcttttct tttacatcca attgggaacg agcaacacaa
    17401 gaagatttta ctccggtgaa tgctgttagc cagattctcc aaaaccacga agctctcctc
    17461 tccgcacata tctaaatcat caatgcatat cgtcaatgga accaccaggg aggcttctat
    17521 tagaaaatcg actatctccc tacagaaatc ctctcttcct tttttgccca aatccaccga
    17581 ctccttcacc gggctaagca gctcaggcac cagaaggctg agaggatggg agtacttctg
    17641 tagtaagaaa tcatgcttat ttttgaggta gggaaagaac tcgtacaata tttgtctgat
    17701 tggttgataa ggtgctaaat ctttatggct gcatcctgcc ataagaaaag gtatcccctc
    17761 aaattgaacg ctcatcttta gcctacgcaa cagagtgctt ttaccgatac ctgcttctcc
    17821 ttgcagcaga actactttcc cttgggcatg gatgctttct cttgcgctct gctctaatct
    17881 cgccaattct ttctcccgac caatcaattc tccgctgtaa atgtagcttg aatagagtcc
    17941 atcttccttt gtgcccaggt ttctctggct caacttggag attctttctt ttacttctaa
    18001 ggcgttcctt ggtcgcccct ccggcagctt atccaggagg tcgagtatta gatcatccag
    18061 ctcgtaggga atggttggat taagatctct cggaggagtt ggttcttcgc ccaagtgccc
    18121 ggccaccacc ctgactggat ctttggaggg gaaaggtagt tgtgaggtgg tcacctgata
    18181 gaggataact cccagggagt agaggtcagt tcggccatct aagcttccac cgctgaagat
    18241 ctctg
//
LOCUS       DASWLN010000173         6104 bp    DNA     linear   ENV 20-FEB-2024
DEFINITION  MAG TPA_asm: Candidatus Zixiibacteriota bacterium isolate
            SMAG_U2949 k141_230659, whole genome shotgun sequence.
ACCESSION   DASWLN010000173 DASWLN010000000
VERSION     DASWLN010000173.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37960594
            Sequence Read Archive: SRR10376091
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Candidatus Zixiibacteriota bacterium (soil metagenome)
  ORGANISM  Candidatus Zixiibacteriota bacterium
            Bacteria.
REFERENCE   1  (bases 1 to 6104)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 6104)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (26-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/12/2024 08:28:26
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 1,440
            CDSs (total)                      :: 1,416
            Genes (coding)                    :: 1,412
            CDSs (with protein)               :: 1,412
            Genes (RNA)                       :: 24
            rRNAs                             :: 1 (16S)
            partial rRNAs                     :: 1 (16S)
            tRNAs                             :: 21
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 4
            CDSs (without protein)            :: 4
            Pseudo Genes (ambiguous residues) :: 0 of 4
            Pseudo Genes (frameshifted)       :: 0 of 4
            Pseudo Genes (incomplete)         :: 3 of 4
            Pseudo Genes (internal stop)      :: 1 of 4
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..6104
                     /organism="Candidatus Zixiibacteriota bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_230659"
                     /isolate="SMAG_U2949"
                     /isolation_source="soil"
                     /db_xref="taxon:2053527"
                     /environmental_sample
                     /country="USA: Oregon"
                     /lat_lon="42.99 N 123.63 W"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            complement(<1..1016)
                     /locus_tag="VGB16_06405"
     CDS             complement(<1..1016)
                     /locus_tag="VGB16_06405"
                     /inference="COORDINATES: protein motif:HMM:TIGR00229.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="PAS domain S-box protein"
                     /protein_id="HEX9917353.1"
                     /translation="MIKKLHLKVMKRKNSKEKLSAEELNSFTNSFASFNKITQSLQSA
                     YRSLEEKFENLNQKLEQANRELRQSLAEKDRISNYLNNILESLTSGVLAINLYGKITL
                     FNRAAEEMLGYKSDEVLGRPYLEIMGKNIKEECAATYLLKSKGSVVNQEKEIYARDGR
                     KIPVGYSITLLSDSEGELIGAVEIFFDLTKLRQLEEEVSRVKTLAALGEMAATVAHEV
                     RNPLGGIAGFAALLDRDIPEEDERKRLVKKIIQGVEILNRTVINLLNYTRVVKLNPTE
                     VDLVKFADEVVAHFNMDISQKMKNITIQRNYPDRRLVCRLDTEQFRQVVLNLLYNAAQ
                     SMPDG"
     gene            complement(1101..1475)
                     /locus_tag="VGB16_06410"
     CDS             complement(1101..1475)
                     /locus_tag="VGB16_06410"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEX9917354.1"
                     /translation="MSEEHNSSQKRKPNLDILAEYEELLEDYNEILKISTKVLDEITK
                     RFNEDTLMPLLEEKLKIADRISKASKQISQIELSGDSPVQVEIARQGKSIIAQIKNRA
                     ELLLKSENRIEEALRAKGLKIK"
     gene            complement(1514..2167)
                     /locus_tag="VGB16_06415"
     CDS             complement(1514..2167)
                     /locus_tag="VGB16_06415"
                     /inference="COORDINATES: protein motif:HMM:NF012301.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_process: GO:0000160 - phosphorelay signal transduction
                     system [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="response regulator"
                     /protein_id="HEX9917355.1"
                     /translation="MAKEKILVVDDEIFVRELLMEFLSKQDFEVILAESGEKAIETIK
                     SNPAQVALIDLKMPGMDGLKTMQEVKRISPHTLAIIMTGYPTIETSIEALRGGAYDYV
                     VKPFKLNELKNAIDRALNEHHLRLEIDELKERIKNLERNLKVYQISGRRSDVSKPQAG
                     SDLSHIAGGALYGVKPKVNDKENSVLEQIRRLGELKEKGFLSQEEFESKKSELLRRL"
     gene            complement(2232..2597)
                     /locus_tag="VGB16_06420"
     CDS             complement(2232..2597)
                     /locus_tag="VGB16_06420"
                     /inference="COORDINATES: protein motif:HMM:TIGR00377.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0043856 - anti-sigma factor antagonist
                     activity [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="STAS domain-containing protein"
                     /protein_id="HEX9917356.1"
                     /translation="MEIVRNTLGDTAVLHLNGRLNLNSSAEAKNRIKELLDKRSRKII
                     LNLTRVDFINSSGLGVLISVLKDIRLANSRLAITNLSPYVHEIFEITQLVNVFDIYDN
                     EEAALDGFLLKSGDMGRLR"
     gene            complement(2631..3038)
                     /locus_tag="VGB16_06425"
     CDS             complement(2631..3038)
                     /locus_tag="VGB16_06425"
                     /inference="COORDINATES: protein motif:HMM:NF024970.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005524 - ATP binding [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="ATP-binding protein"
                     /protein_id="HEX9917357.1"
                     /translation="MEVYRFCFPSNLKYLDRVYDITSKILQDMPIDLMAKRRILTAVS
                     EAVTNSLVHGNKENSDKTVRLTFQQNDGYLQIDVDDQGKGFAPQITSLIESAKKLEER
                     GRGIPIMKAYVDEVRFKYMKRKGMKVSLRKNLA"
     gene            complement(3047..4357)
                     /locus_tag="VGB16_06430"
     CDS             complement(3047..4357)
                     /locus_tag="VGB16_06430"
                     /inference="COORDINATES: protein motif:HMM:NF018884.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="GAF domain-containing SpoIIE family protein
                     phosphatase"
                     /protein_id="HEX9917358.1"
                     /translation="MSELTEIRKYPPEERAQRLEEKLEIEKSRIKDIATLGAVLTSIL
                     DLNQVLSALMEMSIEMVNAEVGAIILSENGKLVTKVFWGVGENTLDSIKTQEGKGIVE
                     WTLETGRTLLINDVHHNQTTASVLPQSHIKCLVSMPISCKEQTIGALVVVNKREEEGF
                     SPEDCFSLEMLVNFAAVAIENSRLLKESLERQKLESQLKIAEEVQKTLLPSQEISLKG
                     ARVDSLYVPAYHIGGDYFDIIPKSEDKFIVVVGDVSDKGIPAALLMTAVRSLVRAEAQ
                     SESPVKEIITRINRLVCEDVIKYKDMFVTLFYGCFDLKTKRLVYSNAGHLPPIWVKSK
                     ANSLEQLTGGGVILGQFEDSKYKEGEIHFDNNDTFFFYTNGVTECANAQGEMFGKERL
                     IQLVKEEKKSGYDDFLKTLEKRVRKFSNDSPEMNDLTMVLVKIQ"
     gene            complement(4887..>6104)
                     /locus_tag="VGB16_06435"
     CDS             complement(4887..>6104)
                     /locus_tag="VGB16_06435"
                     /inference="COORDINATES: protein motif:HMM:TIGR00225.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="S41 family peptidase"
                     /protein_id="HEX9917359.1"
                     /translation="SQLMRGASGTTVKLQIKREGLETPLEYEIERAIIELKNVPYYGV
                     VEDSIGYIRLSRFSEDSGKELREAIGNLNQRKIKGLILDLRNNGGGLLSQAVETSEML
                     LDKGKLVVYTKGRDEKQTIKYLAQAAPIFPDKPLVVLVDEGTASASEIVAGAVQDWDR
                     GVIIGNNTYGKGLVQQIFDLPMNAYIKLTVAKYYIPSGRCIQKPETRNGANVAGNEQE
                     QIQKLEQEEKFYTKGGRLVFGEGGIAPDIQVKEENLSPIQINLEREAVFFDFAVRYAG
                     KHPDLPPNFEVDQKMLDDFTEFLKEINFTYQSPLEVELKNLKKSIQEESVDTSFGLVL
                     ANLENLVKKEKEKDFERSLDDIKYSIRRDILFNLYGEKAYYEQVLLKADPYVKKGIEV
                     LKDKKEYEKVLKS"
BASE COUNT         1529 a         1536 c         1124 g         1915 t
ORIGIN      
        1 ccatccggca tagattgtgc cgcattgtat aataaattca agacaacttg acgaaattgt
       61 tcggtgtcta aacgacaaac caacctgcgg tcaggataat ttcgctggat ggtgatattc
      121 ttcattttct gtgagatgtc catattaaaa tgagctacca cctcgtccgc gaacttcact
      181 aagtccacct cagtcgggtt caacttcaca acccgggtgt aattgagtag attgatgact
      241 gtgcgattta agatttccac cccttgaatt atctttttca ccagtctttt gcgctcatcc
      301 tcttcaggta tatctctatc caaaagcgca gcaaaaccag ctattccacc taagggattt
      361 ctcacctcat gggccaccgt agccgccatc tcacccaaag cagctaaggt cttgacccgg
      421 ctgacttcct cttctaactg tctaagtttg gtcaaatcaa agaaaatctc caccgcaccg
      481 ataagttccc cttcactgtc ggataaaagc gtaatggaat atcccaccgg aatctttctg
      541 ccatctctgg cataaatctc cttttcttga ttgaccactg aacccttcga cttcaagaga
      601 taggttgcgg cacactcttc tttaatattt tttcccatta tctccaggta aggtctgccc
      661 aggacttcat ctgatttgta tcccagcatc tcttcagccg cgcggttgaa taaagtgatc
      721 tttccataca gatttatcgc caggactcct gaggtcaggc tctccaggat gttatttaga
      781 taattcgaga ttctatcttt ctccgccagg ctttgcctca actcccggtt ggcctgttcc
      841 agcttctgat tcagattctc gaatttctcc tctaaacttc gataggcgct ctgcaggctc
      901 tgagtgatct tgttaaagga ggcaaaagaa ttggtaaaag agttaagctc ttctgcggag
      961 agcttttctt tggagttttt cctcttcata acctttaaat gtagtttttt tatcaattat
     1021 aaatataatc caaaatcaac ctttggcaat taaaattgtt acgagtggta agatttggtg
     1081 ttgaaaatat gaacagagct ctatttgatc ttaagaccct tggctcttag ggcttcctca
     1141 attctgttct cggatttaag caaaagctct gccctgttct tgatttgagc tattatgctt
     1201 ttgccctgcc gagcaatttc cacctgaacc ggactgtctc cggaaagctc tatctgagat
     1261 atctgcttgg atgctttgga gatcctatca gctatcttca gcttttcctc caatagaggc
     1321 ataagggtgt cctcgttgaa ccttttggtg atctcatcca aaaccttagt ggaaattttg
     1381 agaatttcgt tgtagtcttc aagcagctcc tcatactcag ccaggatgtc cagatttggc
     1441 tttctcttct gactgctatt atgctcttca ctcatcttgt cttcaatctg aaaagatgct
     1501 caaggattaa cgctcacaac ctacgcaaaa gttccgactt cttgctttcg aactcttcct
     1561 ggcttaaaaa acctttctct ttcaactccc ctaatcttct tatttgctcc aagactgaat
     1621 tttctttgtc gttgaccttt ggcttcacgc cgtaaagagc tccgcctgca atatggctaa
     1681 ggtcggagcc ggcttgtggt tttgaaacat cacttcttcg tccagagatt tgataaacct
     1741 taagatttct ttccagattt tttatccttt ccttaagctc atctatctcc aatctcaagt
     1801 gatgctcatt cagagctcta tcgatggcgt tcttgagctc gttcaacttg aagggcttga
     1861 ccacataatc ataagctccc ccgcgcagag cctcgatcga ggtttcaatg gtaggataac
     1921 cagtcataat gatggctaag gtgtggggag aaattctctt gacctcttgc attgtcttca
     1981 agccatccat gcccggcatc ttcagatcta tcaaggccac ttgggctgga ttggacttga
     2041 ttgtctcaat tgccttctcg cccgactcag ctaagataac ttcgaagtct tgcttgctca
     2101 aaaactccat caaaagctcc cgcacaaaga tctcgtcatc cactaccagg atcttctctt
     2161 tagccataac ttccctcttt gattgaatct ccccgggggt aaacttctta gtgtcccgtg
     2221 aatcgaaaag ctcaccttag ccggcccatg tccccggact taagaagaaa accatccaga
     2281 gccgcttcct cgttatcgta gatgtcaaac acgttaacca gctgagtgat ttcgaatatc
     2341 tcgtgcacgt agggagaaag attggttata gctaatctgc tattagctaa acgaatatcc
     2401 ttgagcaccg agatcaaaac tcccaaaccg gaactgttga tgaaatcaac tcgggttaag
     2461 ttgaggatga tcttcctgga acgtttgtcc agaagctcct tgattctgtt ttttgcctca
     2521 gctgagctat tcagattcaa gcgcccattg aggtgaagca cagctgtgtc ccccagggtg
     2581 ttcctcacga tctccatact atccttttta ttataagcat taagagttgc ctaagcaaga
     2641 ttttttctta agcttacctt catccctttt ctcttcatgt acttaaatct gacttcgtcc
     2701 acatatgctt tcattatagg tatcccgcgc cccctctcct ctagcttctt ggcgctctct
     2761 attaaactag tgatttgcgg cgcaaatccc ttgccctggt catctacgtc aatctgtaga
     2821 tatccatcat tctgctgaaa agtcagcctg acggttttgt cggaattttc tttgttgcca
     2881 tgaactaaac tgttagttac cgcctcgctg actgcggtca gtatccttct tttcgccatt
     2941 aaatctatcg gcatatcttg caatatcttt gacgtgatat cgtacactcg gtccaggtat
     3001 ttgagattgc ttggaaaaca aaacctgtaa acctccatct tcccctctat tgtattttaa
     3061 ccaacaccat ggttaaatca ttcatttccg gggaatcatt agagaatttt cttactcttt
     3121 tttccagggt cttaaggaaa tcatcataac cacttttttt ctcttccttg accagctgga
     3181 ttaacctctc cttgccaaac atctcccctt gagcattggc acactcggtc actccattcg
     3241 tataaaaaaa gaaagtgtca ttgttatcga agtggatttc tccctcttta tatttagaat
     3301 cctcaaattg acctaagatc acccctccac cggtcaattg ctccagggag ttggctttgg
     3361 atttaaccca aataggagga agatggcctg cattggaata aactaatctt ttggttttca
     3421 gatcaaagca cccgtagaaa agggtaacga acatatcctt atatttgatt acatcctcgc
     3481 acaccagacg attgattctg gtaatgattt ccttgaccgg actttcgctt tgagcttcag
     3541 cccgaaccag actgcgaacc gctgtcataa gcagggcagc cggaatcccc ttgtccgaaa
     3601 catctccaac caccacaatg aacttatctt ccgatttggg aatgatatca aaataatccc
     3661 ctccaatatg gtaagctgga acataaaggg aatcgaccct tgctcctttc aaggaaatct
     3721 cttggctggg cagcaaggtt ttttgaacct cctccgcaat cttaagctgg ctttccaatt
     3781 tctgcctttc cagagattcc ttcaaaaggc gtgaattctc aatggccact gcagcgaaat
     3841 taacgagcat ctccaagctg aaacaatcct ctggggagaa accttcttcc tcccttttgt
     3901 tcaccactac taaggcgccg atagtttgct ccttacagct gataggcatt gagaccagac
     3961 acttgatatg cgattgaggc aaaacagatg cagttgtctg attgtggtga acatcattta
     4021 tgagaagggt tcggcctgtc tccaaagtcc actcaacgat cccttttccc tcttgcgtct
     4081 tgatagaatc cagtgtattc tctcctacac cccagaaaac tttagtaacc aacttgccat
     4141 tctcagagag aatgattgct cccacctcgg cattgaccat ctcgatgctc atctccatca
     4201 aggcggacag aacttgattt agatccaaaa tggaagttag caccgctccc agggtggcaa
     4261 tatctttaat tctgcttttt tctatctcta acttctcttc taacctttga gctctctctt
     4321 cggggggata ttttctgatt tcggttagtt ctgacacact cctcccttta tttgacttat
     4381 ctaataagtt tatcgtctca tgaaatctta ttcttgaatt caataggaga tttgtttttt
     4441 tataatttat aactgcttaa tttatcataa cgagttggaa taggttggat aactacaaag
     4501 tggataggcg ggtagcccag cggtatgaat cgagatggtt cgtaccagtg gtattcgcca
     4561 ttgggcaaga atgaacttcc ccgctcaaag ggcggggcct ccaaaacatc cccttcccct
     4621 tgaggggggg gactaacttg aaaatctcgc caagggcgga gagagggtga gaatcatgtt
     4681 cgctatcccc cctcacccta accctctccc ccaagggaga gggaacttat agaaatgcaa
     4741 ttcgttatgg acagacctac ataacataat aggaaggggt gggtaacagt ggccgccatt
     4801 acctaccctt ctcctattaa atcaaaatgc cctccgggaa gatgtggagg gcatttttca
     4861 tctatatatc atataatcaa agcgagttag cttttcaaca ccttttcata ttctttttta
     4921 tctttcaata cttcaatccc tttctttaca taggggtcgg ccttgaggag aacctgctcg
     4981 taatatgcct tttctccata gaggttaaag aggatatctc ttctgattga gtatttaata
     5041 tcatccagac ttctctcaaa atctttttcc ttttcttttt tgaccagatt ctccagatta
     5101 gctaaaacta acccaaagct cgtgtcaacg ctttcttcct gaattgattt cttcaagttc
     5161 ttcaactcca cctccagggg actctggtaa gtaaagttga tttcctttag gaattccgtg
     5221 aaatcatcaa gcattttctg gtccacttcg aagttaggtg gcagatcagg atgtttgcct
     5281 gcatagcgta ccgcaaaatc aaaaaatact gcttccctct ccaggtttat ctggatcgga
     5341 gaaagatttt cctcttttac ttgaatatca ggagcgatac ctccttcacc gaaaactaat
     5401 cgtccgccct tggtgtagaa cttttcctct tgttccagct tctgtatttg ctcttgttca
     5461 tttcccgcaa cattcgctcc gtttctcgtt tccggcttct ggatacatct tccgctggga
     5521 atgtagtatt tagccacggt cagcttaata taagcattca tgggtaagtc gaaaatctgc
     5581 tgcaccaacc ctttgccata agtgttgttc ccgataatca cccctctatc ccaatcctga
     5641 acagcaccgg caactatctc cgaggcagaa gcagtgcctt catccaccag tacgaccaga
     5701 ggtttatctg gaaaaatagg tgcggcttgc gcaagatatt ttatagtctg tttttcatct
     5761 cttcctttgg tatagacaac cagctttcct ttatctaaga gcatctcaga tgtttcaact
     5821 gcctgactta aaagcccacc tccattgttt ctcaaatcca gaatcagccc ttttattttt
     5881 ctttgattca agttacctat cgcctccctc agctctttac cggaatcttc agaaaaacgg
     5941 gaaagcctta tatatccaat gctatcttcc accacaccgt aataaggcac attcttcaac
     6001 tcaatgatag cccgttcaat ctcatactcc aaaggcgttt ctaatccttc ccttttgatt
     6061 tgaagtttga ctgtggttcc gctggcgccg cgcataagct ggga
//
LOCUS       DASWLN010000174         4076 bp    DNA     linear   ENV 20-FEB-2024
DEFINITION  MAG TPA_asm: Candidatus Zixiibacteriota bacterium isolate
            SMAG_U2949 k141_1120427, whole genome shotgun sequence.
ACCESSION   DASWLN010000174 DASWLN010000000
VERSION     DASWLN010000174.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37960594
            Sequence Read Archive: SRR10376091
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Candidatus Zixiibacteriota bacterium (soil metagenome)
  ORGANISM  Candidatus Zixiibacteriota bacterium
            Bacteria.
REFERENCE   1  (bases 1 to 4076)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 4076)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (26-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/12/2024 08:28:26
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 1,440
            CDSs (total)                      :: 1,416
            Genes (coding)                    :: 1,412
            CDSs (with protein)               :: 1,412
            Genes (RNA)                       :: 24
            rRNAs                             :: 1 (16S)
            partial rRNAs                     :: 1 (16S)
            tRNAs                             :: 21
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 4
            CDSs (without protein)            :: 4
            Pseudo Genes (ambiguous residues) :: 0 of 4
            Pseudo Genes (frameshifted)       :: 0 of 4
            Pseudo Genes (incomplete)         :: 3 of 4
            Pseudo Genes (internal stop)      :: 1 of 4
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..4076
                     /organism="Candidatus Zixiibacteriota bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_1120427"
                     /isolate="SMAG_U2949"
                     /isolation_source="soil"
                     /db_xref="taxon:2053527"
                     /environmental_sample
                     /country="USA: Oregon"
                     /lat_lon="42.99 N 123.63 W"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            <1..226
                     /locus_tag="VGB16_06440"
     CDS             <1..226
                     /locus_tag="VGB16_06440"
                     /EC_number="3.4.-.-"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+.
                     GO_function: GO:0046872 - metal ion binding [Evidence
                     IEA];
                     GO_process: GO:0006508 - proteolysis [Evidence IEA]"
                     /codon_start=2
                     /transl_table=11
                     /product="M23 family metallopeptidase"
                     /protein_id="HEX9917360.1"
                     /translation="KDVVGYVGSSGYSTGPHLDYRVRVENKYLNPLKMSLPSAAPIAR
                     SSWEDFCENRNRLISAMETMSQVHAVASSS"
     gene            292..1170
                     /locus_tag="VGB16_06445"
     CDS             292..1170
                     /locus_tag="VGB16_06445"
                     /inference="COORDINATES: protein motif:HMM:NF014533.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003677 - DNA binding [Evidence IEA];
                     GO_process: GO:0030420 - establishment of competence for
                     transformation [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="DNA-processing protein DprA"
                     /protein_id="HEX9917361.1"
                     /translation="MDTLHQRKFIIALREKAKIGPKAFQRLLLSFGSVENIFGASKNE
                     LCNVPRISAEKAEQIIESGEEIDEIESYIQRLEKEGTSICTILEEDYPHILTQINDPP
                     LLLYSKGEFPLRNKKFVAVVGTHHATEEGIKNAVQIGKDLARKEVVVVSGLARGVDSS
                     AHLGAITNGGKTYAVLGNGFNLIYPPENLSLSEEITKNGAIISEYGPDVRVNIGQLMA
                     RNRIVVGLSQAVIMVEMEEGSIGTEDAILRAVEQGKPLFVCQKDGNPNADFLIKEGAI
                     PISGEEDLDLVLNYMF"
     gene            1183..1407
                     /locus_tag="VGB16_06450"
     CDS             1183..1407
                     /locus_tag="VGB16_06450"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEX9917362.1"
                     /translation="MNLERADKIEALKGSVDELCHKIINQEVTSEQATLEMDRVRVEA
                     ENLVPDEMDKFDMIYENRFKRLIQQFLSNA"
     gene            1400..2014
                     /locus_tag="VGB16_06455"
     CDS             1400..2014
                     /locus_tag="VGB16_06455"
                     /inference="COORDINATES: protein motif:HMM:NF013378.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="YigZ family protein"
                     /protein_id="HEX9917363.1"
                     /translation="MPDIADSFSSIRRFNQSQLRVKGSKFIASAFPVENEKEVQDSIG
                     KIRKEFYDATHHSFAYRIKLKEKDIFRASDAGEPKGTAGEPILLTIRSRNLYNILVVV
                     TRYFGGTKLGKGGLSRAYQQAASEVLETSKIVERLITRELEFIFPLNMIGKVTQVLNK
                     YGARILEKDFQTEGKLNIEVRTSQKENLVKALIGVTAGQVKFQK"
     gene            2018..2569
                     /locus_tag="VGB16_06460"
     CDS             2018..2569
                     /locus_tag="VGB16_06460"
                     /inference="COORDINATES: protein motif:HMM:NF013718.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="phosphatase PAP2 family protein"
                     /protein_id="HEX9917364.1"
                     /translation="METITNLDTQLFYFLNLKFENHIFDLVMPILTNLAYWRIPLLLA
                     WIGLMIFGKRKGRIVGILVILVVTIGDQVCNQIIKPWVGRIRPCNVLEGVHLLINCTK
                     SFSFPSSHATNLFSSSFLFSYFYRRWTSIFLMIALLVSYSRIYVGVHYPLDVLGGMVL
                     GFLVTLLVLIAYKSLEKYFLEKR"
     gene            2692..3450
                     /gene="arsM"
                     /locus_tag="VGB16_06465"
     CDS             2692..3450
                     /gene="arsM"
                     /locus_tag="VGB16_06465"
                     /inference="COORDINATES: protein motif:HMM:NF008823.0"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="arsenite methyltransferase"
                     /protein_id="HEX9917365.1"
                     /translation="MSKSKKIKDVVKKKYGSIAKSTGKCCCSTANLETCTPKVTDISH
                     GYNKEELEALPEGANLGLGCGNPLALTEIKSGETVLDLGSGAGIDVFLAAKKVGPKGK
                     IIGLDMTDEMLIKARQNAKKVGIKNVEFKKGEIEDMPIPSNSIDLVISNCVINLVPDK
                     KKAYQEIYRVLKPKGRFSVSDMVTRGNIPEEIKEDMEAWAGCVAGALDKDEYLSIVKE
                     CGFKDLKVKSEVEYDYKRTDEFGLVSMGLVGFKK"
     gene            3467..3601
                     /locus_tag="VGB16_06470"
     CDS             3467..3601
                     /locus_tag="VGB16_06470"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEX9917366.1"
                     /translation="MTRILQDSLYFQFAGEIDKKVYSDILVSPDRNAVVFRSIVKKMT
                     "
     gene            3685..3757
                     /locus_tag="VGB16_06475"
     tRNA            3685..3757
                     /locus_tag="VGB16_06475"
                     /product="tRNA-Asn"
                     /inference="COORDINATES: profile:tRNAscan-SE:2.0.12"
                     /note="Derived by automated computational analysis using
                     gene prediction method: tRNAscan-SE."
                     /anticodon=(pos:3718..3720,aa:Asn,seq:gtt)
BASE COUNT         1301 a          676 c          971 g         1128 t
ORIGIN      
        1 aaaggatgtg gttgggtatg tggggagcag cggatattcc accggacccc atttggatta
       61 tcgagtgaga gttgagaata aatatcttaa tcctctcaag atgtccttac cttcagctgc
      121 cccgattgct cgaagctctt gggaagattt ctgcgaaaat agaaacaggt tgatttccgc
      181 catggagacg atgagccagg ttcacgcggt ggcatcatct tcttgaagaa acagatggca
      241 cttaagttcc cttgatatag ataaccaaaa aagataatta tgtaggatgt tatggatact
      301 ttgcatcaac gtaagttcat aattgccttg cgggagaagg caaaaatcgg tcccaaggcc
      361 tttcaacggt tgcttttatc cttcggctcg gtggagaata tttttggggc aagtaagaat
      421 gaattgtgca atgtgcctcg tatcagtgcg gaaaaagcag agcagattat agagtcaggc
      481 gaagagatag atgaaattga aagttatatc cagagattag aaaaagaagg cacaagcata
      541 tgcactattt tggaggagga ttatccccat attctaacac agataaatga cccgccactc
      601 ttgttgtatt ccaaggggga gtttcctcta agaaacaaga agttcgtagc agtggtgggg
      661 actcaccacg ccaccgaaga gggaataaaa aatgcagtcc aaataggaaa agacttagca
      721 agaaaggaag ttgtggtggt gagcggcttg gcaagagggg tcgattcatc agcgcatttg
      781 ggcgctatca ccaatggtgg aaaaacctat gctgttttgg gaaacgggtt caacttgatc
      841 tatccaccgg aaaatctttc gctctccgag gagataacca aaaacggcgc cataatcagc
      901 gaatacggcc cagatgtgcg ggttaacatt ggtcaactta tggccaggaa tcgcatagtg
      961 gttggtcttt cccaggcagt gataatggtg gagatggaag agggaagcat tggaacggag
     1021 gatgccatat tgcgggcagt ggagcagggg aagccgctct ttgtgtgcca aaaggatgga
     1081 aatccaaatg cagattttct gattaaggaa ggggctattc ccatatcagg cgaagaggat
     1141 ttggatttgg ttttaaacta tatgttttag aggaatactc agatgaattt ggaaagagcg
     1201 gataagatag aggcgcttaa gggctcagtg gatgagcttt gccacaaaat aataaatcaa
     1261 gaggtaactt cggaacaggc cacgcttgag atggatagag tcagggtaga agctgaaaac
     1321 ttagttccag atgagatgga taaattcgat atgatttacg agaatagatt caaaagatta
     1381 atccagcaat tcctttccaa tgcctgacat tgcggatagc ttctcaagta taagacggtt
     1441 caaccaaagc cagcttagag tcaagggctc caaattcatc gcctctgcct ttccagtaga
     1501 aaatgaaaaa gaagtgcaag attcgattgg aaagataaga aaagaattct atgatgccac
     1561 ccatcacagt tttgcttatc gaataaaact aaaagaaaag gatatattca gggccagcga
     1621 tgctggggag ccgaagggaa ctgcgggaga gccaattctt ttgactataa ggtcaaggaa
     1681 tctatataac attctagttg tggttacgcg ttattttggt ggcacgaaat tgggaaaggg
     1741 agggctttcg agggcttatc agcaagcggc aagcgaagtt ctggaaacct ctaaaatagt
     1801 tgaaagatta ataacgagag agcttgaatt tatttttcct ttgaatatga ttggaaaagt
     1861 cacccaggtt ttaaataaat atggcgcgag aattttagag aaagattttc aaacagaagg
     1921 gaaactaaac atagaagtta gaacaagcca aaaagaaaac ctggtcaagg cgttaattgg
     1981 ggttacggct gggcaagtca aattccaaaa atgagctatg gaaactataa ccaatcttga
     2041 tacacagctt ttctattttt tgaacctaaa gtttgaaaat catatctttg atttggtaat
     2101 gcccatattg accaatctgg cttattggag aatacctttg cttttggctt ggattggttt
     2161 gatgatattt ggcaagagaa agggaagaat tgttggaatc ttggtgatat tagttgtcac
     2221 tatcggagat caggtatgta atcagataat caagccctgg gtgggaagaa ttcgtccctg
     2281 caatgtcttg gagggggtgc atctattgat taattgcaca aaatcttttt cttttccctc
     2341 ctctcatgct accaatctgt tctccagctc ttttcttttt agttatttct atagaaggtg
     2401 gactagcatc tttttgatga ttgccctttt agtatcttat tcccgcatct atgttggagt
     2461 tcattatcct ttggatgttt tgggaggaat ggttttggga tttttggtta ctctcttggt
     2521 cttaatagcg tataagagtt tagagaaata ttttttagaa aaaagataaa agtacaaaac
     2581 aaattgtttt tttaatacgt aggggaaacc ttcaaatttg tgcgacggac aggtcgcgac
     2641 ctgtccctac gttaaaaatt aaatccaaaa atcaaaaatc taggaggaat tatgtcaaaa
     2701 tccaaaaaaa tcaaagatgt ggtgaagaaa aaatatggtt caattgccaa atccacaggc
     2761 aaatgctgct gttcaactgc taatttggaa acttgcaccc caaaggtaac cgatatctcc
     2821 catggttaca ataaagaaga gttagaggct ctgcccgaag gggcaaattt gggtctgggt
     2881 tgtggaaatc cattggcttt aacagaaata aaatccgggg agacagtttt ggatttggga
     2941 agtggagctg gcattgacgt ttttttagct gccaaaaagg ttggtcccaa aggaaaaatt
     3001 atcggtttag atatgaccga tgagatgcta atcaaagcaa gacaaaatgc caaaaaagtc
     3061 ggaataaaaa atgtcgagtt caagaagggt gaaattgaag atatgcccat cccgtcaaac
     3121 tcaattgatt tagtcatttc aaactgcgtt ataaatttag tgccggataa gaaaaaagct
     3181 tatcaggaga tttatcgggt attaaaacca aagggaagat tttccgtgtc tgatatggta
     3241 actcggggga atatacctga agaaatcaag gaagatatgg aggcttgggc gggttgtgtg
     3301 gctggagcat tggataagga tgaatatcta agtatagtga aggaatgcgg ttttaaagat
     3361 ttgaaagtca aatccgaagt tgaatatgat tacaaaagga cagatgagtt cggactggtc
     3421 agcatgggac tggttggatt taaaaaatag gaacttgtaa tgatagatga cgcgtatact
     3481 tcaagattct ttgtattttc agtttgcagg cgagatagac aagaaagttt atagcgatat
     3541 tttagtcagc ccggatagaa atgcagttgt gttcagaagc atagtcaaga agatgacgtg
     3601 atgatatgtt cttgatgatt aagtcttgac accgtcatct aaacctttat atttatattt
     3661 tagaaaacag aaaaaacctc atactcccgc gtagctcagt cggcagagca ggtggctgtt
     3721 aaccaccgtg tcgctggttc gattccggcc gcgggagctt ttttgagctg cttttgaaag
     3781 agcttgcccg taaaccagaa cattcgtact taattttgtc atttctcttg ataaagaaac
     3841 tgctttggat tatcttccac ataatatcat aatcaattta gcgtcatcaa ttcaaaacct
     3901 caaaaaacgg agttaaaatg aaaaggcaga accgaaccat ttctgttttg ttggcaggga
     3961 taatcatatt tttctctttt tcagctattt tcgcccaaac cccaaacaag ggaattccgc
     4021 aaagaagtga tattccggaa aagtataaat ggaaattaga agacatttac gctact
//
LOCUS       DASWLN010000175        11031 bp    DNA     linear   ENV 20-FEB-2024
DEFINITION  MAG TPA_asm: Candidatus Zixiibacteriota bacterium isolate
            SMAG_U2949 k141_1120917, whole genome shotgun sequence.
ACCESSION   DASWLN010000175 DASWLN010000000
VERSION     DASWLN010000175.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37960594
            Sequence Read Archive: SRR10376091
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Candidatus Zixiibacteriota bacterium (soil metagenome)
  ORGANISM  Candidatus Zixiibacteriota bacterium
            Bacteria.
REFERENCE   1  (bases 1 to 11031)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 11031)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (26-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/12/2024 08:28:26
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 1,440
            CDSs (total)                      :: 1,416
            Genes (coding)                    :: 1,412
            CDSs (with protein)               :: 1,412
            Genes (RNA)                       :: 24
            rRNAs                             :: 1 (16S)
            partial rRNAs                     :: 1 (16S)
            tRNAs                             :: 21
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 4
            CDSs (without protein)            :: 4
            Pseudo Genes (ambiguous residues) :: 0 of 4
            Pseudo Genes (frameshifted)       :: 0 of 4
            Pseudo Genes (incomplete)         :: 3 of 4
            Pseudo Genes (internal stop)      :: 1 of 4
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..11031
                     /organism="Candidatus Zixiibacteriota bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_1120917"
                     /isolate="SMAG_U2949"
                     /isolation_source="soil"
                     /db_xref="taxon:2053527"
                     /environmental_sample
                     /country="USA: Oregon"
                     /lat_lon="42.99 N 123.63 W"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            710..1939
                     /locus_tag="VGB16_06480"
     CDS             710..1939
                     /locus_tag="VGB16_06480"
                     /inference="COORDINATES: protein motif:HMM:TIGR01730.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005215 - transporter activity [Evidence
                     IEA];
                     GO_process: GO:0006810 - transport [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="efflux RND transporter periplasmic adaptor
                     subunit"
                     /protein_id="HEX9917367.1"
                     /translation="MRKIFIIIVAVPVLTLLLFFILKEKNKKSEAKYITAEVQKGDIS
                     IMVTATGSLEAVTTVQVGSQVSGTILALYADFNDLVKKGQIIAQLDPTFLKAQVAQAQ
                     ADLEKAVASAELSKKDYERTLSLFEKNMVSESNKEISLTNYELAKAQEKSAQASLDRV
                     KTNLDYATIVSPINGVVISRNVDVGQTVAASLSAPTLFTIAQDLTRMQVKTSVDEADI
                     GSVREGQEVIFTVDAYPEQSFKGTVKQVRLSPEVVQNVVSYDVIIELSNPDLLLKPGM
                     TANVTILIDQRLDVLKVASGALRFRPAVERKKTSSGTKEANPMARNLSSPDTSAVKSN
                     PIGLTGAGQKALWILDPQGKPKPVPVQIGISDGTFTQIISDNLKEGDKVITGQKGVES
                     PSNNNQQVNPFTPRFGH"
     gene            1952..2695
                     /locus_tag="VGB16_06485"
     CDS             1952..2695
                     /locus_tag="VGB16_06485"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_006982343.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_function: GO:0016887 - ATP hydrolysis activity
                     [Evidence IEA];
                     GO_function: GO:0042626 - ATPase-coupled transmembrane
                     transporter activity [Evidence IEA];
                     GO_function: GO:0140359 - ABC-type transporter activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="ABC transporter ATP-binding protein"
                     /protein_id="HEX9917368.1"
                     /translation="MNQLILTEDLVKIYKLGDYEIHALRGINLSIEKGEFVAIMGPSG
                     SGKSTLMHILGCLDRPTKGSYLLEGKDISSLDRDNLAQIRNQKVGFVFQSFNLLSRTT
                     ALENVELPLLYNGTTSKQNMQKAKKALATVGLEGRENSFPNQLSGGQQQRVALARALI
                     NDPAIILADEPTGNLDTRTSIEVMEIFQSLNENGITIILITHEKDIAAYAKRNIAFRD
                     GKIVKDEIVSTPRKAKDELLILPQEEEGI"
     gene            2692..3909
                     /locus_tag="VGB16_06490"
     CDS             2692..3909
                     /locus_tag="VGB16_06490"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_013610673.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ABC transporter permease"
                     /protein_id="HEX9917369.1"
                     /translation="MRYLQILKVAYKALGRNKMRSSLTMLGIIIGVGAVIAMMGIGQG
                     AKKMINDQIASLGDNLLTIFPGSHMSGGMRAGAGTRTSLTDEDAKAIKDNCSAVLRVS
                     PEVRTGAQVVAGNLNWGTSIEGYSTDFPLIRSWPLFSGSFFTEQDVKGATKVCILGKT
                     VVDNLFSGQDPVGEIIRIKKLPFRVIGVLSPKGQNAFGHDQDDMIIVPYTTAQKKIAG
                     ITHVNSIIASALDRTEIDLAQQQITQLLRQRHKILPGQDDDFTVRSQLDIASAAGSTS
                     SVMTILLAAIASISLIVGGIGIMNIMLVSVTERTREIGIRMAVGAKGKDILIQFLIES
                     IVLSLIGGLIGIILGVVCSQLISVILHWSIYTSVPAVVLSFLFAAFVGIFFGFYPARK
                     ASLLDPIEALRYE"
     gene            complement(4768..5535)
                     /locus_tag="VGB16_06495"
     CDS             complement(4768..5535)
                     /locus_tag="VGB16_06495"
                     /inference="COORDINATES: protein motif:HMM:NF013245.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0016020 - membrane [Evidence IEA];
                     GO_function: GO:0140359 - ABC-type transporter activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="ABC transporter permease"
                     /protein_id="HEX9917370.1"
                     /translation="MNLSYRIYYVFFRNLVSYKRFILPTFVVSLGQPLFYLITFGIGM
                     GAYMGYFGGKPYLNFLVPGVLVSSVMLSSTFECLYGTFVKVSYQKLFDSLIATPVSAE
                     DAIAGEIAWGAFRGLVSGSLMLVVATFMHVFPVSIVNVLLLLVLMIFIGILFGSLAMI
                     VTSFAPNFDFFNYYTELIITPMLFFSGVFFPLDRFPGWMKVFAKFLPLTHAVNISRSA
                     FSGVPSPGLALDFLYIILLEAVAFYIGIKLMKRRLIK"
     gene            complement(5569..6477)
                     /locus_tag="VGB16_06500"
     CDS             complement(5569..6477)
                     /locus_tag="VGB16_06500"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_020522343.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005524 - ATP binding [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="ATP-binding cassette domain-containing protein"
                     /protein_id="HEX9917371.1"
                     /translation="MIVAKDLTKKYGDFTAVDGISFEIKEGECFGFLGPNGAGKTTTI
                     RMIHCVSPVTSGTVTVLGKKSHIDNREVKLMTGVIPQEINLDPDLTVYENLVIFAKFF
                     DIPRAEIKKRVAELIGFVELESKRNSKIDELSTGMKRRLLVARALLNQPRLIIADEPT
                     TGLDPQARHLIWERLRSLKSRGTTLILTTQYMEEAEQLCDRLMVMYQGRILKQGSPRQ
                     LVSDEIGREVVEIRIAKEEDEKLIAQMADVSCGHERVGDTLYFYCRDGHEVMKKIVDF
                     NLPNVLNRPATLEDVFLKLTGRTLIE"
     gene            complement(6621..8087)
                     /locus_tag="VGB16_06505"
     CDS             complement(6621..8087)
                     /locus_tag="VGB16_06505"
                     /inference="COORDINATES: protein motif:HMM:NF015039.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008234 - cysteine-type peptidase activity
                     [Evidence IEA];
                     GO_function: GO:0070005 - cysteine-type aminopeptidase
                     activity [Evidence IEA];
                     GO_process: GO:0006508 - proteolysis [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="C1 family peptidase"
                     /protein_id="HEX9917372.1"
                     /translation="MKWLSLLAGLVILFTFYNLSYAAPGITPELIQQMQKENAPDSVT
                     LRLIDAVSKNGIKNVALNQAIIKQHDPYFSHEIKTAKITNQEQSGRCWLFGGLNVLRP
                     YILKKLNIDDFEFSQAYLFFWDKLEKSNTFLEEVIKRRNMDIRDEDFQRILEKPLDDG
                     GWWNYFVYLVKKYGLVPKSVMPETESTKNSETMDRLLEQKLMDYAFQLRQMNQEGKTL
                     DDMEKTKEKFLSEIYHMLCFHFGTPPEKFQFRYKPKSTEEEKDKKDKDKKDKEKKEDK
                     EKSTLTEFVTYTPQEFAGKFVTENLEGYVVLANIPSRDYNQMYSLENARNIYDIEDFK
                     FLNVRLEDLKKVTLNSVLGDEPVWFVSDVSHQIDFTSGIMHPQIYLYKDIYGIDIKLP
                     KERSILYRNVTTNHVMVFIGVDVVNEKPTKWLVENSWGEKVGKDGYLHMYDSWFDEYV
                     IVSVVKREFVPENIKPFLDTKPIIIKEKDTLGKFLEMR"
     gene            8421..9032
                     /gene="lexA"
                     /locus_tag="VGB16_06510"
     CDS             8421..9032
                     /gene="lexA"
                     /locus_tag="VGB16_06510"
                     /EC_number="3.4.21.88"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003503971.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004252 - serine-type endopeptidase
                     activity [Evidence IEA];
                     GO_process: GO:0006282 - regulation of DNA repair
                     [Evidence IEA];
                     GO_process: GO:0006355 - regulation of DNA-templated
                     transcription [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="transcriptional repressor LexA"
                     /protein_id="HEX9917373.1"
                     /translation="MADKLTNRQRQILEYISEMINRKGFPPTIREIGEKFAFKSTNAT
                     RSVLAALSKKGYIKRKPLVSRGIELLRQTKASCSVVPLLGRISAGIPILAIENIEGNF
                     AIDKSFLPDRESFFLKVVGDSMKDAGIFHGDYVLAQSQEIANKGDIVVAIIGDEATVK
                     KYFPEKNKIRLEPANQEYGPIIVEKNSPDFRIAGKVVGLLRRM"
     gene            complement(9378..10502)
                     /locus_tag="VGB16_06515"
     CDS             complement(9378..10502)
                     /locus_tag="VGB16_06515"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="N-acetyltransferase"
                     /protein_id="HEX9917374.1"
                     /translation="MQTEKLFIIPVQTKKELRQFILLPWKIYRNDKNWVPPLVSERKD
                     FLNPQKNPFFKHAEVKLFLAKKGNEVVGRIAGIINHLHIQTHNERCGFFGLFECIENY
                     EVAKALLDTVKAWLKSKGMEKMRGTADFSSNDQWGFLLEGFDSPPAFLMPYSPKYYLE
                     FMEKYGLKKAKDLYAYRMDTDERPPQRIYKVAERIRKKENLVIRKVDLDDFENEVKKI
                     KEIYNQAWSRNWGAIPMTDEEFSHMAKDMKQFVDRDLVFIAEVDGQPAGFSLALPDLN
                     EALIRINGRLFPFGLFKLLYYVKRIKNVRLITMGVIQKYQKRGIDTVFYTETFDNGVK
                     KGYNGGELSWVLEDNVLMNKAIGLMNTRLYKKYRIYEGEV"
     gene            complement(10854..>11031)
                     /locus_tag="VGB16_06520"
     CDS             complement(10854..>11031)
                     /locus_tag="VGB16_06520"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011553761.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0030170 - pyridoxal phosphate binding
                     [Evidence IEA];
                     GO_process: GO:0009058 - biosynthetic process [Evidence
                     IEA]"
                     /codon_start=2
                     /transl_table=11
                     /product="aminotransferase class I/II-fold pyridoxal
                     phosphate-dependent enzyme"
                     /protein_id="HEX9917375.1"
                     /translation="KVWKALLEEGVFTNPIISPAVPPGSALLRTSYTATHTDEQLNKA
                     LEIFEKVGKKMGII"
BASE COUNT         3108 a         2466 c         2303 g         3154 t
ORIGIN      
        1 tgagttgact aaaaggttga agtggccccc aggcccaccg ccacatccac caggggagag
       61 gtaattagtt agcaattgaa actctgaata gagcgactca cgcgctaaca aatgtttcat
      121 tagagaagaa ataagtatgg attcccgatt agaccctcgg gaatgacaaa acatagtagg
      181 ggcgtaaggc aatacgcccc tacacacagc aagaacgacg cctgtccttc gacaggctca
      241 ggatggtgag caaagtcgaa ccataaaggc atggaaatct attgataatg taaaaaggtg
      301 gatttttgct tttgctcttt tggataaaaa aatcatctat aaaacttaaa cctacgtatt
      361 ataattaaac ctttaaataa taaaatatgc tgctttgtca aggaaatatt acttccattc
      421 tctgtcattc tgacccgtat ggggaagaat ctcgcttgcc tcccattgtg cccagattct
      481 tcactccgct agcgctccat tcagacttgt cctgagtgaa gtcgaaggaa tgacaagaac
      541 ggagcctgcc ctgagtgaaa cgaatgggtt cagggtgaca atattatcac atcattaatt
      601 agttgacaaa gcaatatgcc agataaagat agaacttttt gctttttagt ttgtatttaa
      661 tgtaatcaat taatatttta tctgagctta tgagtagaaa ggaaattcta tgagaaagat
      721 tttcattata attgtcgcgg ttccggtttt gacattgttg ctctttttca ttctcaagga
      781 aaaaaataag aaatccgagg cgaagtatat caccgctgaa gtacaaaaag gggatatctc
      841 tattatggta actgccactg ggagcttgga ggcggtaacc acagttcagg taggaagcca
      901 ggtctccggc accatactag ccctttatgc ggatttcaat gaccttgtaa aaaaagggca
      961 gattattgcc cagctggacc cgactttctt gaaagcccag gtggcacagg ctcaggcaga
     1021 tttagaaaaa gctgtggctt cagccgagct ttctaaaaag gactatgagc gaaccttatc
     1081 gctgtttgag aaaaacatgg tatccgaatc taataaagag atctctttaa ccaattacga
     1141 actggcaaaa gcccaggaaa aatcagccca ggctagttta gaccgggtta agactaatct
     1201 tgattatgcc accatagtct ctcccataaa tggagttgtc atctccagaa atgtggatgt
     1261 aggacagact gtagcagcga gtttatcggc gcccactctt tttactattg cccaagacct
     1321 aaccagaatg caggtcaaga ccagcgtaga tgaggcagat atcggaagcg taagggaggg
     1381 tcaagaagtg attttcactg tagatgctta tccagaacaa tcctttaaag gaaccgtaaa
     1441 gcaggtaagg ctttctcctg aagttgtgca gaatgtggta tcctatgatg taatcattga
     1501 actctcaaac cccgatctgc tgttaaaacc cggaatgacc gccaacgtaa ctatactaat
     1561 agaccaaaga cttgatgtgc tcaaagtcgc ttcaggggct ctgcgttttc gtccagcagt
     1621 ggagaggaaa aaaacttctt caggaacaaa agaggcaaac ccgatggctc gtaatctgtc
     1681 ttcacctgat accagcgcag ttaaatcaaa tcccattggt cttacaggag caggtcaaaa
     1741 agcgctctgg attcttgatc ctcaaggtaa acctaagccg gttccggttc agattggaat
     1801 atccgatggc accttcaccc agattatatc ggataatctt aaagagggag ataaggtaat
     1861 caccggacaa aaaggcgtag agtctccttc taataataac cagcaggtta acccctttac
     1921 tcccagattt ggccattgat aggagttaaa attgaatcag ctcatactta cagaggattt
     1981 ggtcaaaatc tacaagctcg gagattacga gattcatgcc ctccggggca taaacctatc
     2041 cattgaaaag ggtgaattcg tagccataat gggtccttcg ggctcaggga aatcaacctt
     2101 gatgcatatc ttgggttgct tagatcgacc caccaaaggt agttacctac ttgaaggtaa
     2161 agatatatcc tctttggacc gagataactt ggcccagata agaaaccaga aggttggttt
     2221 tgtttttcag tccttcaacc ttctttcccg aacaaccgcc ttggaaaatg tagaactacc
     2281 tctgctttac aacggcacca cctcaaagca aaacatgcaa aaagctaaga aagctttggc
     2341 cacggtgggc ttggaaggaa gggaaaattc ttttcctaac cagctctccg ggggtcagca
     2401 gcaaagagtg gctttggcta gagcattgat caatgatcca gccataatct tggcagacga
     2461 acctactgga aatctggata ccaggacttc catcgaggtg atggagatat ttcaatccct
     2521 taatgagaat gggattacta ttatacttat aacccatgaa aaagatattg ccgcctatgc
     2581 taagaggaac atcgccttcc gggatggcaa aatcgtcaaa gatgaaatag tctccactcc
     2641 cagaaaagcc aaagatgagc ttttgatttt gcctcaagaa gaggaaggaa tatgagatat
     2701 ttacagattc tgaaagttgc ctacaaggca ttgggtagga acaagatgcg ttccagcttg
     2761 acgatgctgg gtataataat cggggtgggg gcggttatcg ctatgatggg aatagggcag
     2821 ggtgcaaaaa aaatgatcaa tgatcagata gcttccttag gagataatct tttaaccata
     2881 ttcccgggaa gtcatatgag cggaggtatg agagctggcg ctggaacgag aacctcttta
     2941 acagatgaag atgctaaggc gatcaaggac aactgctctg cagttctgag ggtttcaccg
     3001 gaggtaagaa ccggagcaca ggtcgtagca ggaaatctca actggggaac atctattgaa
     3061 gggtattcaa cagattttcc tcttatccgc tcctggccct tattctcagg ctcttttttc
     3121 acagaacaag atgtaaaagg agccactaag gtctgtattt tagggaaaac tgtagttgat
     3181 aaccttttca gtgggcagga ccctgtcggg gagatcataa gaatcaaaaa actacctttc
     3241 agggtaatag gggttttatc cccaaaagga cagaatgcct ttggccatga tcaggatgat
     3301 atgattattg tcccttatac cactgcccaa aaaaaaattg ctgggattac ccacgttaat
     3361 tctattatcg cctcagccct ggaccggact gaaatagatt tagctcaaca gcagataacc
     3421 cagctgcttc gtcagagaca caagattctt ccaggacagg atgatgactt cactgtcaga
     3481 agtcagttgg atattgcctc tgcggctggt tcgacctcta gtgtaatgac catcctttta
     3541 gctgctatcg cctccatctc cttgatagtc ggtggtatcg gaataatgaa catcatgctg
     3601 gtttccgtga ctgagagaac cagggagata ggaatcagga tggcagttgg agccaaaggc
     3661 aaagatatat tgattcaatt tttgatagaa tccattgttt taagtctgat tggaggtttg
     3721 attggaataa ttttaggagt tgtatgctct caattaatct ccgtgatttt gcactggtca
     3781 atttatacct cagttcctgc agtagtttta tccttcctct ttgccgcttt tgtggggatt
     3841 ttctttggtt tctatcctgc cagaaaagcc tcccttttgg atccgataga ggcgttaaga
     3901 tacgagtaaa tatttttgag gtatttgtag ggacagaaca ctgttctgtc ctcttttctt
     3961 attggaaacc atcaagtgtc caatagtagg gacggggtca ccctacccaa taaattacac
     4021 agcgagcccg ccctttggcg ggtcgcccct acgaacaaac gccaaactta caaactccaa
     4081 actgtatttc agaggtggaa ctggttgaca accctttttg atttatcaag cagtaggtta
     4141 ggagtagctt ctgacaggaa ttcccacgcg gcctcaaatg cacccatagg gccatacgac
     4201 aacaccattt tcgtcgacat agacaaggcg acgaagaacc cgtttgttga ggctgggcga
     4261 atacgcctca tgatcgacct gccagtagaa acgatccttc caggtgtgaa aaggacgtga
     4321 cgtaagatat gtccaatctt cttctttggg gaactcagcg gctgtggtgt caacaaaaac
     4381 tatgtaggac tcaaaggcta cccgaacgtc tgaggtgtcg tggacggaga ccccgctcgt
     4441 gtagagagtg tctacgccca tccaacacaa atctggttga gtcggattat cacagttgaa
     4501 tatcataaca aggcagataa atatagaaaa gagaataacg gctagataag ggaatcggct
     4561 caatactctg gtcaatgcgt tactccaatc aatataactt cagaaagagt gtatatccta
     4621 tcgtgttttg tcggcccatt cattcaatat attgtaagca tttattcaat cctgtcaact
     4681 acattctaaa ttggaataat ctcaagtgta tagtgctttc tgtctcttac tgtcgacatt
     4741 ggttctctta ctgacccttt ccgaccgcta ttttatcaat cttcttttca tcaacttaat
     4801 tccaatgtaa aaagcaacgg cttccaagag aatgatgtac aaaaagtcca gtgccaaacc
     4861 cggagaaggt acgccggaaa aagcggagcg cgaaatattg accgcatgcg taagaggaag
     4921 gaacttggca aataccttca tccatcccgg gaatctgtca agcgggaaaa agactcctga
     4981 gaaaaacagc atcggggtaa tgatcaactc ggtatagtaa ttgaagaagt cgaagttcgg
     5041 cgcgaacgat gttacaatca tagccagcga gccgaacaaa atgccgatga atatcatcaa
     5101 gaccaaaaga agcagcacgt tgactatcga gaccggaaac acgtgcatga acgtagctac
     5161 gaccagcata agggaaccgc tgactagtcc ccgaaaagct ccccaggcta tttctccggc
     5221 tatcgcatcc tcagccgaca cgggtgtggc tatcagagaa tcaaacagct tttggtagga
     5281 taccttgacg aaggtgccgt ataaacactc aaaggttgac gagagcatca ccgaagatac
     5341 tagaacgccg ggcacgagaa aattcagata cggcttcccg ccaaaatatc ccatatacgc
     5401 gcccatgcct ataccgaagg tgataagata gaagagcggc tgccccaggc tgactacgaa
     5461 cgtgggcaga atgaaccttt tatacgaaac aaggtttcta aaaaagacgt aatatatccg
     5521 gtacgaaaga ttcattttat gcttccttga catgctacac gccgtacttt attctatcaa
     5581 agttctgccg gttaatttga gaaacacatc ctccaaagtg gctggccggt tcaacacatt
     5641 cggaagattg aaatctacga tttttttcat tacctcatgt ccgtcacggc aataaaaata
     5701 gagagtatct cccacccgct catgtccgca agaaacatcc gccatctggg cgataagctt
     5761 ttcatcctct tctttggcta tccttatttc aactacttcc ctgcctatct catcagaaac
     5821 cagctgcctg ggggatcctt gtttcaggat gcgcccctga tacataacca tcaggcgatc
     5881 acagagttgc tctgcctctt ccatgtattg ggtggtaagt attagtgtcg tgccccggct
     5941 tttcagcgat ctcaatcttt cccagattag atgtctggct tgcgggtcaa gtccggtggt
     6001 gggctcatcc gcaatgatga gtctcggctg gtttagaagc gcccgtgcca ccaaaagcct
     6061 ccgcttcatt ccggtggaga gctcatctat cttgctgttc cgcttgcttt ccagctcaac
     6121 gaacccaatg agctcggcaa cccttttttt aatctcagcc cggggaatat caaagaactt
     6181 ggcgaaaatc acgaggttct cataaaccgt caggtcgggg tcaaggttga tctcctgggg
     6241 tatcactccg gtcatcaact tgacctctct attatcgatg tgcgacttct tccccaaaac
     6301 ggttaccgtg ccggaagtga cgggggatac gcagtggatc atccttatgg tggtggtttt
     6361 tccagctcca ttaggtccca aaaaaccaaa acactcccct tccttgattt caaacgatat
     6421 gccatcgacg gcagtgaaat cgccatattt cttagtaagg tcttttgcga ctatcattgt
     6481 gttcatccat agatcctttt tagaagaaac ttaattgtcg tgtctgggac ccgtacagtt
     6541 tcgtgggtca cctgccctgc gcacctgaca aataaactgt gttaggggct tggtgctcct
     6601 gacctattat ctctactcct tcacctcatc tccaaaaatt tccctaaggt atccttctct
     6661 ttaattataa tcggcttggt atccaagaag ggctttatgt tttcagggac gaattctctt
     6721 ttcaccactg agactataac atattcatcg aaccacgaat catacatatg caaataacca
     6781 tctttgccca ccttctcgcc ccagctgttc tccactaacc atttagttgg cttttcattc
     6841 acaacatcaa cccctataaa gaccataacg tggttagtcg tcacgtttct gtaaagtatg
     6901 ctcctctcct tgggaagctt gatatctatt ccatagatat ctttataaag gtaaatctgc
     6961 ggatgcataa tgccggacgt aaaatcaatt tggtggctta catctgagac aaaccagacc
     7021 ggctcatccc ctaaaacaga attcaaagtg acttttttca aatcctcaag ccgcacatta
     7081 aggaacttaa aatcttcgat atcatagatg ttgcgcgcat tttccagact atacatctgg
     7141 ttataatctc tggaagggat atttgccaaa accacatatc cttccagatt ctctgtcacg
     7201 aacttacccg caaactcctg cggagtgtaa gttacaaatt cggttaaagt cgatttttcc
     7261 ttatcctctt ttttctcttt atctttttta tctttgtcct ttttatcttt ttcctcttcg
     7321 gtcgattttg gcttatagcg gaactggaac ttttctggag gggtgccaaa atgaaagcaa
     7381 agcatatgat agatctcaga aagaaacttc tctttggttt tttccatatc atccagggtt
     7441 tttccttcct ggttcatctg tcttaattga aaagcataat ccatcagttt ctgctccaat
     7501 aaacggtcca ttgtttctga atttttggtg ctttctgttt ccggcatcac tgattttgga
     7561 acaagtccat attttttcac caaatagaca aagtaattcc accatccgcc atcatccagg
     7621 ggcttttcta aaatcctctg gaaatcctca tcccgtatgt ccatatttct tctttttatg
     7681 acctcttcca aaaacgtatt tgatttctcc agcttgtccc agaaaaacag ataagcttgg
     7741 gagaattcaa aatcatcgat attcaacttc tttaagatat atggtctaag cacgttcagc
     7801 ccaccaaaaa gccaacatct gccggattgt tcctggttgg ttatcttggc ggttttgatt
     7861 tcgtgactaa aataagggtc gtgttgtttg attatagctt gatttaaagc tacgtttttg
     7921 atgccgtttt tggacaccgc atcaattaga cgcaaggtta cactatccgg agcattttcc
     7981 ttttgcattt gctggataag ttcaggtgtt attcccggag cagcataact cagattatag
     8041 aatgtaaata aaatcactaa accagctaaa agacttaacc acttcattcg tacctccttt
     8101 attagtttca cctgtttaaa gaatatagga ttatttatcg aattttcaat caattttcac
     8161 taaaaaatta tgaaacgtgg ctcttctgga gtgtcaagct tcagcttgac tattgaattg
     8221 tcacgggtct tatgttttat tcgtcccgcc agaggcggga cactccaaaa atcctatctc
     8281 aattttcttc ttgacgcctg ctcatttgag cagtatcttg tgctaagttc atggaaaaat
     8341 tatgaaccta agtctttaat cccctctccc tcgaggggag agggtcaggg tgaggttgaa
     8401 tatttattga ggagaacgaa atggcagata aattgaccaa ccgccagaga caaattctgg
     8461 aatacatctc cgagatgata aacagaaaag gattccctcc aaccataagg gagattggtg
     8521 aaaagttcgc cttcaaatcc accaacgcca cccgctcggt cttggctgct ttgtctaaaa
     8581 aaggttatat caaaagaaaa cctttggtct caaggggaat tgagctttta agacaaacca
     8641 aagcatcatg ttctgttgta cctcttttgg gaagaatctc cgccggcatt cccatcttag
     8701 caattgagaa tattgaagga aactttgcaa ttgataagtc tttcttgccg gacagggaat
     8761 cttttttctt aaaagtggtg ggggatagta tgaaagatgc cgggatattt catggagatt
     8821 atgtcttggc tcaatcccaa gagatagcca acaaaggtga tatcgtggtt gccataatcg
     8881 gagatgaagc cacagtgaaa aaatattttc ccgaaaaaaa caaaatccgc ttagagcccg
     8941 ccaatcaaga gtacggacca attatcgttg aaaaaaacag tccggatttc aggattgccg
     9001 ggaaggtggt ggggttgttg aggaggatgt gagagaaata aacgtagggg cggtcaaatg
     9061 accgcccgtt tcggacagac ctaaaggtct gtccctacat ctttttattt gtcattcctg
     9121 ttttatttgt cattcctgcg caggcaggaa tccattggtt cttgttaaca cgaacccgct
     9181 ttgctttaca ctgtcattgc gagcaacccc gatctatcag ggggcgaagc aatctcgctc
     9241 tctacttcaa gattgcttcg tcgcttcact cctcgcaatg acaatcttgt gtgcctatta
     9301 ggccccataa cctaaataga atatcctgtt gggttgggga acaaaggcag gcacagagaa
     9361 atacagtata tttttagtta cacctccccc tcataaatcc tatatttctt atataacctc
     9421 gtattcataa gccctattgc cttgttcatc aagacattat cctctaaaac ccagctcagc
     9481 tctcccccgt tgtatccttt tttgactccg ttatcaaaag tctcagtata gaatacagta
     9541 tcgattcccc ttttctgata tttctgtatt acgcccatag ttatcagtct tacgtttttt
     9601 atccttttga catagtacaa aagcttaaaa agcccaaagg gaaataacct tccatttatc
     9661 cttatcaaag cttcattcag atccggtaga gcaagagaaa atccagctgg ctggccatcc
     9721 acttctgcga tgaaaaccaa atccctatca acgaattgtt tcatatcctt tgccatatgg
     9781 gagaactcct catctgtcat tggaattgcc ccccagtttc tgctccaggc ctgattgtaa
     9841 atctctttta tctttttaac ttcattctca aagtcatcta aatctacttt tcttattacc
     9901 agattctcct tctttcttat cctttccgcc actttataaa ttctttgggg tggtctttca
     9961 tcagtatcca tgcggtaagc atagagatcc tttgcctttt tcaaaccata tttctccata
    10021 aactccaaat aatatttcgg actataaggc atcaaaaaag ccggcgggga atcgaagccc
    10081 tccagaagaa atccccactg gtcattggag gaaaaatcag ccgtacctct cattttctcc
    10141 atgcctttgg atttgagcca agctttgaca gtatctaaca gggcttttgc tacttcgtaa
    10201 ttttctatgc attcaaaaag cccaaagaac ccacatcttt cattgtgggt ttgaatgtgc
    10261 aggtgattta taatacctgc aatcctgccc acaacttcat ttcctttttt ggctaagaat
    10321 aatttgactt cagcgtgttt aaagaatggg ttcttttgtg gatttagaaa atctttcctt
    10381 tcggaaacca gtggcggcac ccagtttttg tcatttcgat agattttcca ggggaggagg
    10441 ataaattgtc ttaactcttt tttggtctgg acgggaatta tgaaaagctt ttcagtttgc
    10501 ataattttcc ttatttgttg ttattttctt tatttgtcat tgcgaggagg ccttcgacat
    10561 agctcaggac gacgaagcaa tctttctttt atcttgagat tgtcattcct gcgtaggcag
    10621 gaatccattt atttttaaaa ccagattccc gctggagttt atcccgtctt tggcgggacg
    10681 ggaatgacat gcaagaaaac aagacggcgt atcctttata gcgcctattt gttattcgta
    10741 ttcccgcctt gccctcaagg cggggatttt cacccacgtt gcctgcccgg ccagggaggg
    10801 caacgtgggt ttactggtta tttatataag tgtaggtaac atcaagcgaa aaactaaatt
    10861 atccccattt tcttccccac cttctcaaaa atctccaatg ctttattcag ctgttcatcc
    10921 gtgtgggtgg cggtgtagct ggtgcgaagt aaggcgcttc cgggtgggac tgcggggctt
    10981 atgattgggt tggtgaatac cccctcttcc aaaagggctt tccagacttt g
//
LOCUS       DASWLN010000176         5232 bp    DNA     linear   ENV 20-FEB-2024
DEFINITION  MAG TPA_asm: Candidatus Zixiibacteriota bacterium isolate
            SMAG_U2949 k141_234771, whole genome shotgun sequence.
ACCESSION   DASWLN010000176 DASWLN010000000
VERSION     DASWLN010000176.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37960594
            Sequence Read Archive: SRR10376091
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Candidatus Zixiibacteriota bacterium (soil metagenome)
  ORGANISM  Candidatus Zixiibacteriota bacterium
            Bacteria.
REFERENCE   1  (bases 1 to 5232)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 5232)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (26-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/12/2024 08:28:26
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 1,440
            CDSs (total)                      :: 1,416
            Genes (coding)                    :: 1,412
            CDSs (with protein)               :: 1,412
            Genes (RNA)                       :: 24
            rRNAs                             :: 1 (16S)
            partial rRNAs                     :: 1 (16S)
            tRNAs                             :: 21
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 4
            CDSs (without protein)            :: 4
            Pseudo Genes (ambiguous residues) :: 0 of 4
            Pseudo Genes (frameshifted)       :: 0 of 4
            Pseudo Genes (incomplete)         :: 3 of 4
            Pseudo Genes (internal stop)      :: 1 of 4
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..5232
                     /organism="Candidatus Zixiibacteriota bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_234771"
                     /isolate="SMAG_U2949"
                     /isolation_source="soil"
                     /db_xref="taxon:2053527"
                     /environmental_sample
                     /country="USA: Oregon"
                     /lat_lon="42.99 N 123.63 W"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            35..1360
                     /locus_tag="VGB16_06525"
     CDS             35..1360
                     /locus_tag="VGB16_06525"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_008084894.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="cyclic 2,3-diphosphoglycerate synthase"
                     /protein_id="HEX9917376.1"
                     /translation="MPKRKVIIMGAAGRDFHNFNTVHRHNRTYQVVAFTATQIPNIYG
                     RKYPAKLAGKLYPKGIPIFPEEDLIKSIEKDKVDEVVFSYSDVSYDYVMHRAALVNSA
                     GADFKLLGAKETMLESKVPVVAVCATRTGSGKSQTTRRVCQILKELGKKIVVVRHPMP
                     YGDLAKQACQRFAQLSDLDKHNCTIEEREEYEPHIAQGNIVYAGVDYHLILKKAEKEA
                     QVIVWDGGNNDTSFFKPDLYIVVADPHRPGDELHYYPGSTNLILADIVVINKVGTAKK
                     EDVAQVRSNVGMVNPKAIIIEAESPIFVDNPALIKDKNVLVVEDGPTLTHGGMKYGAG
                     VIAAQRYGAKTLIDPRPFAIGSIKEAFKKFPHMGSLLPAMGYGDGQIEELERTINSAK
                     AEVVVIATPIDLRRVLKIEKPSVRVTYELQEISKPDLKDILTDFVMKLA"
     gene            1414..2367
                     /gene="arcC"
                     /locus_tag="VGB16_06530"
     CDS             1414..2367
                     /gene="arcC"
                     /locus_tag="VGB16_06530"
                     /EC_number="2.7.2.2"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_006927224.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008804 - carbamate kinase activity
                     [Evidence IEA];
                     GO_process: GO:0006520 - amino acid metabolic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="carbamate kinase"
                     /protein_id="HEX9917377.1"
                     /translation="MKKRSKTAVVALGGNAITRQDEEDTIAHQFANTRQSLGGVVELV
                     RDGYNLAITHGNGPQVGNAILRVELARDKAPILPLGICVADTEGGMGYMIEQSLQNRL
                     LREKIQRKVTAIITQVIVDKNDPSMQNPTKFIGQFYTNIEAKKSARERGWVVKKDANR
                     GWRRVVPSPLPLEIVEKDTIKSLVGSGVIVITAGGGGIPVYVEEDGSFEGVDAVIDKD
                     RASAVLARDIGAELLLILTGVEKVALNYGTVLQRDLDKITLTETKKYLSEGHFPSGSM
                     GPKIEAAISFLESGGKEVIITSIEKAYLSVKRKAGTRIVAE"
     gene            2817..4184
                     /locus_tag="VGB16_06535"
     CDS             2817..4184
                     /locus_tag="VGB16_06535"
                     /inference="COORDINATES: protein motif:HMM:NF025112.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="HDIG domain-containing protein"
                     /protein_id="HEX9917378.1"
                     /translation="MQGLDNNIIEKILEKGKIYEVGGGVRDKSISPILPDVDKDYLVT
                     GIPLEELCQILSKFGKVDLVGKSFGIIKFVPRKKKEETIQTYDIALPRKEYSTGVGHK
                     DFEVDFDENLRVEDDLARRDFTINAMALELPNEKLIDPLNGRKDIKNRLVRFTSPQSF
                     KDDPLRMLRGIGFAARFEFEIEKKTYDSLCENVSLISTVSMERIQEEINKLLLRAKRP
                     STGFRLMQKSGMLEILFSELEKTVGIDQPGGYHAYDVFEHSILSVDYAPVDLTIRLAS
                     LFHDISKPETKFLKENGSKFYGHDKKGAKVAEDILKRLKYSNEIISTVKLLIDKHMFT
                     TGVTDKGVRRLVNRVGKENIFKLLDLRRADVEAQGKGGETRDVDELEQRIRLEMDKKS
                     PFGLKDLEIDGNDVMEKFNLTPGPKVGKTLKYLLEKVLDEPELNHKDILLKEAKSFLK
                     TIQKE"
     gene            4188..>5232
                     /gene="sucC"
                     /locus_tag="VGB16_06540"
     CDS             4188..>5232
                     /gene="sucC"
                     /locus_tag="VGB16_06540"
                     /EC_number="6.2.1.5"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_012683135.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003824 - catalytic activity [Evidence
                     IEA];
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_function: GO:0046872 - metal ion binding [Evidence
                     IEA];
                     GO_process: GO:0006099 - tricarboxylic acid cycle
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="ADP-forming succinate--CoA ligase subunit beta"
                     /protein_id="HEX9917379.1"
                     /translation="MKIHEYQAKGIFAKYGIPVQKGEVATTPDKVKEIAEKFGKPVVI
                     KAQVLVGGRGKAGGIKIAQNPQEAFGHAQSILGMEIKGLKVKKVLVTECADIAGEAYV
                     GVIVDRSAKKPVIMASPAGGIDIEEVAAKTPEKIFKLYVDPITGLALFHARDLAYKVY
                     ADFKLACQAADIIFKLYKMFWEIDASLTEINPLITTTQGELLALDAKINIDDSALERH
                     PEIEKMRDLDAEEKGEIEARKAGLSFVKLEGNIGCIVNGAGLAMATMDLVKYYGGEPA
                     NFLDIGGSSNPDKVVAAMSIILKDPNVKAILFNIFGGITRCDDVAQGIVYAVNKLNPR
                     VPIVIRLTGTNEDQ"
BASE COUNT         1642 a          940 c         1352 g         1298 t
ORIGIN      
        1 tgccgacttg aaattaaaaa ccaaaggatt atttatgccc aaaagaaaag taattataat
       61 gggggcagct ggaagagatt ttcacaactt caatactgtt catcgccata atcgaactta
      121 ccaggtagta gctttcactg ccactcagat acccaatata tatggcagaa aatatcctgc
      181 gaaactggct ggcaagttat atcctaaggg aattcccatt tttcctgaag aggacttgat
      241 caaatcgata gagaaagaca aggtggatga ggtggttttt tcttacagcg atgtttccta
      301 cgattatgtt atgcatcggg cagctttggt aaattcagct ggagctgatt tcaaacttct
      361 gggcgccaaa gaaactatgc ttgagtccaa ggttccagtt gtggcagtct gtgccaccag
      421 aacaggtagc gggaaaagcc aaactaccag aagagtatgc caaatcctga aagaattagg
      481 gaagaagata gtcgtggtga gacatcctat gccctatggg gacctggcga aacaagcctg
      541 tcaacgcttt gctcagcttt ctgatttaga caagcataat tgcaccattg aggaaagaga
      601 agaatacgag ccgcatatag ctcaaggcaa tatcgtttat gccggggtgg attaccacct
      661 catactgaaa aaagctgaaa aggaagccca ggttatagtt tgggatggtg ggaataatga
      721 cacctctttc tttaagcctg atttatatat cgtagtagct gacccccatc gtcctggaga
      781 tgagttgcat tattatcccg gcagcacaaa tctgatattg gctgacatag tggtgataaa
      841 caaggttgga acagccaaaa aggaagatgt agctcaggtg aggtccaatg ttggaatggt
      901 taatcccaag gcgatcataa tagaagccga gtcaccgatc ttcgtagata accccgctct
      961 aatcaaggat aagaatgttt tggtagtgga agacgggcca actctaaccc atggtggtat
     1021 gaagtatgga gccggggtca tagccgcaca aagatatgga gcgaaaaccc tgattgaccc
     1081 tcgtcccttt gccatcggta gtattaaaga agctttcaag aaattccctc atatgggttc
     1141 tctccttcca gcaatggggt atggagatgg tcagatagag gaattggaga gaaccataaa
     1201 ttccgccaaa gcagaggttg tggtcatcgc cactccaatt gacttgagga gggtattgaa
     1261 gatagagaag ccctcggtta gagtaaccta cgaattgcag gagataagta agccggattt
     1321 aaaagatatt ctgactgatt ttgtaatgaa gctcgcttaa agttttcaaa cgaccttgct
     1381 tgaggaaacc tccagcctta tttcctttaa gttatgaaaa agagaagtaa gaccgcagtg
     1441 gtagccctgg gcgggaatgc cattacccga caggatgagg aagataccat tgctcatcaa
     1501 ttcgccaata cacggcagag cttaggtggc gtcgtggagc tagttcgcga tggatacaac
     1561 ttggctatca ctcacggcaa tggaccacaa gtcggaaatg ctatattgag agtggaatta
     1621 gctcgcgata aagcccccat tttgccttta ggcatatgcg tggctgatac tgagggtgga
     1681 atgggatata tgattgagca atctctgcaa aatcgcttgc ttcgggaaaa gatacaaaga
     1741 aaagtaaccg ctatcataac ccaagtcata gttgacaaga atgacccttc aatgcagaat
     1801 ccgactaaat tcatcggtca gttctatacc aatatagagg caaaaaagtc tgccagagaa
     1861 agaggatggg tagtcaaaaa ggatgcgaat cgtggctgga gaagggttgt tccatcacct
     1921 ttaccgttgg agatagtaga aaaagatact ataaaatctt tagtggggag tggggttatc
     1981 gttatcaccg caggtggtgg tgggattccg gtttatgtag aagaagatgg tagttttgag
     2041 ggagtggatg cggtaattga taaagataga gcttcggctg tcttggctcg ggatatagga
     2101 gctgaactgt tgcttattct gactggtgtg gaaaaggttg ctctgaatta tggtacagtc
     2161 ctgcaaaggg atttggataa gataacctta accgaaacta agaaatatct ttccgaaggc
     2221 catttccctt caggcagtat gggaccaaaa atagaggcgg ccatcagttt tttggagtcc
     2281 ggaggtaaag aggtaataat cacctccatc gagaaagcat atctttcagt aaaaagaaaa
     2341 gctgggacaa gaatcgtggc ggagtaaata aaatcaaaat gcaaaaagaa aattgaaaag
     2401 taaggttttg ttattctttc ctctcccttg aggggagagg ataaaagaaa gggtgaagat
     2461 atgttccgcg tgcacaggga tgaattccct gtgctacgag gttattcttt ttcgtaggtg
     2521 tgggaatttc cttcgacctt gctcaggatg gtgaacttgt tgaaccatat cccacaccgt
     2581 ccatgaataa ggtttgggtt tttttgggca ggcgcaggga cctgtcccta caaaaaaacg
     2641 agacacaaca aatgtagggc aaacctttag gtttgcagat ggaatccgca gggctaaagc
     2701 cttgccctac ataatatgca ttttgtaagg gtttggaatc ttgaagacgc ccaataaatt
     2761 gggcaactac agaaaaacaa caataaaaat gcaaagccgt tcgcaggtta gacaatatgc
     2821 aaggtttaga taataacatc atcgagaaaa tattagagaa ggggaaaatc tacgaggtgg
     2881 gtggaggggt gagggacaaa tctatttctc ctattctgcc agatgtggat aaggattatc
     2941 tggtaactgg aatcccttta gaagagttat gtcagatcct gtccaagttc ggaaaggtgg
     3001 atttggttgg aaaatcattt ggcataataa aatttgttcc tcgaaagaaa aaagaggaga
     3061 ctatccaaac ttacgatatc gccttgcctc gaaaagaata tagcaccggt gtggggcata
     3121 aagatttcga agtggatttc gatgaaaatc tgagagtgga ggatgaccta gctcgccgcg
     3181 atttcaccat caacgctatg gcattggaat tgcccaatga aaaattgatt gaccctctga
     3241 atggcaggaa ggacataaaa aacaggcttg ttcgtttcac ctccccccaa tctttcaaag
     3301 atgacccctt aagaatgcta cgggggattg gttttgcggc gagattcgag ttcgaaatcg
     3361 aaaagaagac ctatgattca ctttgcgaga atgtgagctt aatttcaact gtttctatgg
     3421 agagaatcca ggaagaaata aataaacttc tgctcagagc caagcgtccc agcaccggct
     3481 ttagattgat gcagaaaagt gggatgctcg agattctgtt ttcggaattg gagaagacag
     3541 tgggaattga ccagccaggt ggttatcatg cttatgatgt gtttgagcat tcgattttga
     3601 gtgtggatta tgcaccagtt gatttgacta tccgtctagc gtctcttttt cacgatatct
     3661 ccaagccgga aacgaagttt ttaaaagaga acggctccaa attctatggg catgacaaga
     3721 aaggtgcaaa ggttgcagag gatattttaa aaagactgaa atactcaaat gagataatct
     3781 ccacggtaaa gcttttgatt gataagcata tgttcaccac tggagttaca gataagggag
     3841 tgagaaggtt ggtcaatcga gtgggcaagg aaaatatctt caagctcctg gacttaaggc
     3901 gggcggatgt tgaggcacag ggaaaaggtg gcgagaccag agatgtggat gaattggagc
     3961 agagaataag attggagatg gacaagaagt caccttttgg tttgaaggat ttggaaatcg
     4021 atggcaacga tgtaatggag aaattcaact taactcccgg accaaaagtc ggcaagacat
     4081 tgaagtatct tttggaaaaa gtattagatg agccagaatt gaatcataag gacatacttt
     4141 taaaagaggc aaaaagtttt ctaaaaacaa ttcaaaagga gtagtggatg aagatacacg
     4201 agtatcaagc caaaggtatt tttgccaagt acgggatacc agtgcaaaaa ggggaagtgg
     4261 ctaccactcc ggataaagta aaggaaatag cggagaaatt cggcaagccg gtagtgataa
     4321 aagctcaagt gctggtgggt ggcagaggga aagctggagg aataaagatt gcccaaaatc
     4381 cgcaagaggc attcggtcat gcccagagca tccttggaat ggagattaag ggattgaagg
     4441 tgaagaaggt cttggtaacg gagtgcgctg atattgcagg tgaggcttat gtgggggtga
     4501 tagttgatag aagcgccaaa aagccggtga ttatggcttc gccagctgga ggaatagaca
     4561 tcgaagaagt ggcagccaaa acgccggaaa agatattcaa gttgtacgtt gaccccataa
     4621 ctggattagc tctttttcat gctcgcgatc tggcttacaa ggtttatgct gatttcaaat
     4681 tagcctgcca ggcggcagac ataatcttca aattatacaa gatgttctgg gaaatcgatg
     4741 cttccttaac agagataaat cccttaataa ctacaacgca aggagagctt ctggctctgg
     4801 atgccaaaat aaatatcgac gattccgctt tggaaaggca tccggaaatt gagaagatga
     4861 gagatctgga tgcggaggaa aagggcgaga tagaggcaag aaaggcggga ttgtcttttg
     4921 tcaaattgga aggaaatatc ggttgcatcg tcaacggcgc gggactggct atggcaacta
     4981 tggatttagt gaaatactac ggaggagagc cagccaattt cttagacatt ggaggaagct
     5041 cgaacccgga caaggtggta gccgcaatga gcattatctt gaaagacccc aatgtcaagg
     5101 ctattttatt taacatcttc ggcggaatca cgcggtgtga cgatgtggca caggggatag
     5161 tgtatgcggt caataaactg aacccgagag ttcccatcgt tatccgtttg accggaacaa
     5221 atgaagacca ag
//
LOCUS       DASWLN010000177         1920 bp    DNA     linear   ENV 20-FEB-2024
DEFINITION  MAG TPA_asm: Candidatus Zixiibacteriota bacterium isolate
            SMAG_U2949 k141_1126293, whole genome shotgun sequence.
ACCESSION   DASWLN010000177 DASWLN010000000
VERSION     DASWLN010000177.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37960594
            Sequence Read Archive: SRR10376091
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Candidatus Zixiibacteriota bacterium (soil metagenome)
  ORGANISM  Candidatus Zixiibacteriota bacterium
            Bacteria.
REFERENCE   1  (bases 1 to 1920)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 1920)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (26-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/12/2024 08:28:26
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 1,440
            CDSs (total)                      :: 1,416
            Genes (coding)                    :: 1,412
            CDSs (with protein)               :: 1,412
            Genes (RNA)                       :: 24
            rRNAs                             :: 1 (16S)
            partial rRNAs                     :: 1 (16S)
            tRNAs                             :: 21
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 4
            CDSs (without protein)            :: 4
            Pseudo Genes (ambiguous residues) :: 0 of 4
            Pseudo Genes (frameshifted)       :: 0 of 4
            Pseudo Genes (incomplete)         :: 3 of 4
            Pseudo Genes (internal stop)      :: 1 of 4
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..1920
                     /organism="Candidatus Zixiibacteriota bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_1126293"
                     /isolate="SMAG_U2949"
                     /isolation_source="soil"
                     /db_xref="taxon:2053527"
                     /environmental_sample
                     /country="USA: Oregon"
                     /lat_lon="42.99 N 123.63 W"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            <1..227
                     /locus_tag="VGB16_06545"
     CDS             <1..227
                     /locus_tag="VGB16_06545"
                     /inference="COORDINATES: protein motif:HMM:NF024245.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=3
                     /transl_table=11
                     /product="helix-hairpin-helix domain-containing protein"
                     /protein_id="HEX9917380.1"
                     /translation="EAHKLIVFSLPLDLNRVSAEDLCLIPGVGESLAKEIVTYREKRK
                     GFRSVEELKNVKGIGENKYQSFKTFFIIRP"
     gene            530..1759
                     /locus_tag="VGB16_06550"
     CDS             530..1759
                     /locus_tag="VGB16_06550"
                     /inference="COORDINATES: protein motif:HMM:NF024583.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="GAF domain-containing sensor histidine kinase"
                     /protein_id="HEX9917381.1"
                     /translation="MEESEVKIREDVLIALSRVSAAISGLWDLEAILKVGLNSVLEIM
                     HGVTGGVMLLDEQTKTLSYFVYHGLSAKYAEEMRFKLGEGIAGKVAQNGRAILLEDIS
                     LEPSAARPDLIKTEGLKAFLSVPLRAKDKILGVLNIASTMVRHFTKDDMYLLGSIGDQ
                     LGIAIEQANLYKRLKDANKRYQTLLRQMITIQEEERKRIARELHDETSQQLTALTLNL
                     QALIEMMEMGDAKDPEIKAMLKKSHNIAVQAHTEVARLIRELRPTLLDTLGLPAAIRN
                     LAEIHLASRGINVTTEFKGMEQRRLSHEAELTLFRVAQEALSNIVRHSEAKKAIIRLE
                     CNSNECILCIEDDGKGFNVSEITRIEKDGRGAGLFGVRERVAVVGGEAYIESQPGKGV
                     RATAKVPVSQEHELCQK"
     gene            1747..>1920
                     /locus_tag="VGB16_06555"
     CDS             1747..>1920
                     /locus_tag="VGB16_06555"
                     /inference="COORDINATES: protein motif:HMM:NF012301.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_process: GO:0000160 - phosphorelay signal transduction
                     system [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="response regulator"
                     /protein_id="HEX9917382.1"
                     /translation="MPKIRVLVVDDHTIVRDGICALLRLAGDIEVVGEAANGREALEL
                     VRKFIPDVMLIDIA"
BASE COUNT          570 a          348 c          484 g          518 t
ORIGIN      
        1 tggaggctca caaactcatc gtcttctccc ttcctcttga tctcaatcga gtctctgccg
       61 aagatctctg tctcatcccc ggggtcggag aatccttggc caaagaaatt gtgacctacc
      121 gggaaaagag aaaaggattc cggtccgtcg aagaactgaa aaatgtgaag ggaattggtg
      181 aaaacaaata ccaatccttt aaaacgtttt tcatcataag gccttaatcc ccactgaatc
      241 gggatgtttt tttacagtat ttcggaagta gtaaatattt tatggattaa ggttctaaaa
      301 gatatggtct acctttaata tattccaaaa aagatttatc aggagggtag gtagacccta
      361 tgaaatttga aatctcttgt ggtgttagta tattaaaaga gttcatttct atctcatatg
      421 tatttgaatg agtacatggc attgacagaa aatgggattt cgtatatttt taatttaatt
      481 aatttttttc gtaggttgag aaaacgattt cagtaatgga ggaccaagaa tggaagagag
      541 cgaggttaag attagagaag atgtgcttat agctttgagc cgcgtctcgg cagcaataag
      601 tggcctctgg gatctggaag ctattcttaa ggttggcttg aatagtgtgc ttgagattat
      661 gcacggggtt actgggggcg ttatgctttt agatgagcaa acaaagaccc tatcctactt
      721 tgtctaccat ggtctttctg ccaaatacgc cgaggagatg cgtttcaaac tcggtgaggg
      781 aattgctgga aaggtagctc aaaatggcag ggccatactg ttagaggaca tctcccttga
      841 accaagtgct gcccgtcctg atttaataaa gacggaaggt ttgaaagcat tcctaagcgt
      901 tcctcttcgg gcaaaagaca agatcctggg ggtgctgaat atcgctagca ctatggtgcg
      961 gcacttcact aaagatgata tgtaccttct gggctcgatc ggggatcagc ttggcatagc
     1021 tattgagcag gcaaacctat ataaaaggtt aaaagatgct aataaaagat accaaaccct
     1081 gcttagacaa atgattacta tccaggaaga agaacgaaaa aggattgcca gagagctaca
     1141 tgatgaaacc agtcaacagc ttactgccct gacgcttaat ctgcaagcac ttattgaaat
     1201 gatggaaatg ggggatgcca aagatcctga gatcaaggca atgttaaaaa agagtcataa
     1261 tatagcggtt caggcacata ctgaagtagc cagacttata agagaactcc gccccacttt
     1321 attagataca ttaggactgc ctgccgctat tcgcaatctt gctgagattc atctcgcttc
     1381 cagaggcatt aatgttacta cagaatttaa aggaatggag caacgtcgtt tatctcatga
     1441 agctgaactg actcttttcc gcgtcgcaca ggaagctttg agcaatattg tgaggcattc
     1501 tgaggcgaaa aaagctatta tccgcttaga atgcaactcc aatgagtgta tcttatgtat
     1561 cgaggatgat ggaaaaggat tcaatgtcag cgagatcaca cgcattgaga aggatgggcg
     1621 tggtgccggt ctgtttggtg tgagagaaag ggtagcggtg gtaggtggcg aggcttatat
     1681 tgagtctcag cctggaaaag gggttagagc caccgctaag gtcccagtta gtcaggagca
     1741 cgagctatgc caaaaataag ggtgctggta gtggatgacc acactatcgt gcgtgatggc
     1801 atttgtgcct tgctgaggtt agcaggggat atagaggtgg tgggtgaggc tgccaatgga
     1861 agggaagcat tggagttggt cagaaaattt atacccgatg taatgcttat agacatagct
//
LOCUS       DASWLN010000178         3526 bp    DNA     linear   ENV 20-FEB-2024
DEFINITION  MAG TPA_asm: Candidatus Zixiibacteriota bacterium isolate
            SMAG_U2949 k141_1246446, whole genome shotgun sequence.
ACCESSION   DASWLN010000178 DASWLN010000000
VERSION     DASWLN010000178.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37960594
            Sequence Read Archive: SRR10376091
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Candidatus Zixiibacteriota bacterium (soil metagenome)
  ORGANISM  Candidatus Zixiibacteriota bacterium
            Bacteria.
REFERENCE   1  (bases 1 to 3526)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 3526)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (26-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/12/2024 08:28:26
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 1,440
            CDSs (total)                      :: 1,416
            Genes (coding)                    :: 1,412
            CDSs (with protein)               :: 1,412
            Genes (RNA)                       :: 24
            rRNAs                             :: 1 (16S)
            partial rRNAs                     :: 1 (16S)
            tRNAs                             :: 21
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 4
            CDSs (without protein)            :: 4
            Pseudo Genes (ambiguous residues) :: 0 of 4
            Pseudo Genes (frameshifted)       :: 0 of 4
            Pseudo Genes (incomplete)         :: 3 of 4
            Pseudo Genes (internal stop)      :: 1 of 4
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..3526
                     /organism="Candidatus Zixiibacteriota bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_1246446"
                     /isolate="SMAG_U2949"
                     /isolation_source="soil"
                     /db_xref="taxon:2053527"
                     /environmental_sample
                     /country="USA: Oregon"
                     /lat_lon="42.99 N 123.63 W"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            <1..255
                     /locus_tag="VGB16_06560"
     CDS             <1..255
                     /locus_tag="VGB16_06560"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEX9917383.1"
                     /translation="DVLGGLETAIELAKKSAKIPPEREVEIVELPQKGLFRLDFLKPK
                     LAGFKTKEELEEDRDLIYIKMVSQNQGRPLFMVPPDLYPE"
     gene            complement(515..1351)
                     /locus_tag="VGB16_06565"
     CDS             complement(515..1351)
                     /locus_tag="VGB16_06565"
                     /inference="COORDINATES: protein motif:HMM:NF012622.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="dockerin type I repeat-containing protein"
                     /protein_id="HEX9917384.1"
                     /translation="MRKFNILICFLVLFFLSTTTSWSADSVCGDANGDTSVSVSDVTF
                     LINYLFKGGPTPQLLTSDVNGDFRITVSDVVYLVNYLFKSGTMPACAQVGAIKGRIYL
                     NGAGPCCNSHGCYFANCPDANISYCSIMNPYPKVKVSAFSQGNLIEKTFTDQAGNFYL
                     RLPEGSYSIKIYPPQDEIDSLTNVVVVNYEETDLGQPHYIQFFVYQVLELSSYATQER
                     LQQIMTETGNQIIDYEPNYHLYSIEVPEQYHPQEMIEILMHNYPDDIIWAEVSGYPCP
                     DQ"
     gene            complement(1731..2051)
                     /locus_tag="VGB16_06570"
     CDS             complement(1731..2051)
                     /locus_tag="VGB16_06570"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEX9917385.1"
                     /translation="MKRSVALIIIGVFLFGSLAFLSCDQEKALERYLANPEVANLMMQ
                     KMWANPDLRIKMVDMMTADTASMQMMMDKIVVSPEVSKMMVDKLAANEACYKIMQEAT
                     KKKK"
     gene            complement(2532..3017)
                     /locus_tag="VGB16_06575"
     CDS             complement(2532..3017)
                     /locus_tag="VGB16_06575"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_014866380.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="TspO/MBR family protein"
                     /protein_id="HEX9917386.1"
                     /translation="MAKTNFSRILKLLASIIVCQLAGFVGSIFTGPAIPSWYANLTKP
                     SFVPPNWIFGPVWISLFLLMGIAAFLVWQKGFGDRKVRISLGIFAVQLLLNTLWSILF
                     FGLKSPILGFFDIVLLWIAILLTIIYFARVSKAAAILLLPYIFWVSFAALLNFSIWRI
                     N"
BASE COUNT         1040 a          687 c          829 g          970 t
ORIGIN      
        1 gatgttttgg gtgggttgga gaccgcaatc gagttagcta aaaagtcagc caagattcca
       61 ccagaaaggg aggtggagat tgtggagttg ccacagaagg ggctttttag gcttgatttc
      121 ttaaagccca aacttgcggg atttaagact aaggaggagc tggaggaaga tagggatttg
      181 atctacataa agatggtctc ccaaaatcag gggagaccac tttttatggt tcccccggat
      241 ttgtatccgg aataaaagag ctggagaggt ttttggagat ggtgaggata aaaaagcagt
      301 tttgagtttc gagttttgcc ctgcaccata cggtgcaggg tttgagtttt gagagatacc
      361 ctcaccctaa ccctctcccg taaagggaga gggaatgtgg aaacagaaac acaggcgacc
      421 ctacgggtcg cctgtgagaa gaaaatgtag ggacaggtta gaagacctgt ccgttttttt
      481 cggacaagaa aattacaagg agatataaat gcccttattg atcaggacaa ggatatccac
      541 taacctcagc ccagatgata tcgtcaggat agttatgcat cagtatctca atcatttcct
      601 gtggatggta ttgttcaggc acctcaattg aatacaaatg gtagtttggc tcataatcta
      661 ttatctggtt gcccgtttct gtcattatct gttgtaatct ctcttgggtg gcataagagc
      721 tcagctccag aacctgatag acaaagaatt gaatgtaatg tggttgtccc aaatcggtct
      781 cctcatagtt taccaccaca acgttggtaa gggagtcgat ctcgtcttgc ggagggtaga
      841 tttttatgct atagcttcct tcaggaagcc gcaggtagaa attccctgct tggtcagtaa
      901 atgttttctc gataagattt ccttgactga aagccgatac ttttactttt ggatatgggt
      961 tcataatgga acaataagag atgtttgcat ccggacaatt cgcgaagtag catccatgtg
     1021 aattacaaca ggggcccgct ccatttaagt agattctccc tttaatcgct ccaacttgag
     1081 cacaggctgg cattgtgccg ctcttgaaaa gatagtttac cagatagacc acatcactta
     1141 cggtaattct aaaatctcca ttaacatccg aggtcaaaag ttgaggggtt ggacctccct
     1201 tgaacaaata gttgataaga aacgttacat ctgagacgct tacgctagta tccccatttg
     1261 cgtccccgca aacagagtcc gctgaccagg atgtggttgt tgacaaaaaa aataggacca
     1321 agaaacagat aaggatatta aattttctca tattttttag ccccttatgg ttagaagttg
     1381 acgaaaggaa ctatgataat aaatagctaa gatttgggat ttgtcaagaa aaaaaagaag
     1441 gcgacattat tagttggcgt caacgtgggt ttatccccac cttgagggca aggtgggatt
     1501 actggttctg tcgagtcggg cggagataaa ctccgcccct acgcagttta agttttgtga
     1561 gtttaatttc gaacaatctg aatgagattt agtagggaca gaacactgtt ctgtccctac
     1621 atttgagcaa attaaatata atcagaattc tgttcttata cagggattgt tttcaaaata
     1681 aaaaaggcat ccacatttgg atgccttttt atgttcgatt cttctgtgta ctattttttc
     1741 ttcttggtag cttcttgcat tatcttgtaa caagcctcgt tggcagccaa cttatccacc
     1801 atcatcttag acacttccgg gctaacgaca atcttgtcca tcatcatctg catagatgct
     1861 gtgtccgccg tcatcatatc caccatcttg attctcaggt ctggattggc ccacatcttc
     1921 tgcatcataa gattggctac ctccggattg gctaagtacc tctcaagtgc cttttcctgg
     1981 tcgcaagata agaaagccaa ggagccaaaa aggaaaacac caataataat cagagcgact
     2041 gatcttttca tatttcacct ccttcgaatg atcttaattg atttggttgg aatgacttta
     2101 agtttagctt aaaaaaaact tttgtcaagt gtttttatgg aataaaaccg tttagaatat
     2161 gtaacccagt gcgaatgaat ttattaattt tagtttttag aagaagagga aggttttata
     2221 tgcgccgtgc tctgcactgg gctggatcct tcgacaggct caggatggtg acagttcgac
     2281 cttgtccttc aacaagttcc cttcggtctg agctcagggt cgaagacagg acggtgagcg
     2341 aagtcgaacc gctcactgtc cctgagctta ttgaagagtc gaaggatcca atcatgtgcg
     2401 gggctgaatg ccccgcacta cgaagaacac agcagagacg agaatcctgt aggcgacccg
     2461 tctgggtcgc ctgtgattta atataaattg ggagagctgt caggagtagc tcctgacgga
     2521 acctaaaaaa gtcagtttat tctccagatg gagaaattga gaagtgcggc aaagcttacc
     2581 caaaagatat aaggcaaaag cagaatcgct gctgcttttg ataccctggc aaaataaatt
     2641 atagtcaaaa gtatggctat ccagagcagg acgatatcaa aaaatcccaa gatgggggat
     2701 ttcagtccga agaagagaat cgaccataag gtattaagaa gtagctggac agcaaaaatc
     2761 cccaaagaaa ttctgacttt tctgtcgcca aatccttttt gccagacaag aaatgcggca
     2821 atacccatca gcaaaaataa gcttatccat actggcccaa agatccagtt cggtggaaca
     2881 aaggagggtt tggtaagatt tgcataccaa ctgggaatag ctggcccggt aaaaatggaa
     2941 ccaacaaaac ctgccaattg acagacaatt atgcttgcca agagtttgag aatacgagaa
     3001 aaattcgttt tcgccatctc ataccccctt tgaattggtt tggttttgga gaggggcgaa
     3061 caaaattaga tttccttttt aatcttggct atgtggagtg aaaagtcaag gggaataaaa
     3121 agtaaaattg aaaatgacaa attgaaaatt gaagatgaaa aaatgaattt gttgcttgtc
     3181 attcccgtga aaatgcgaat tctgcatctt gagacaggaa tgcattggga gagggggttg
     3241 tagttgccca atttattggg cggttttttt aattcgctcg atccttcgac aagctcagga
     3301 tgggtgagtc tgccgaacca tgaatcgagc aactacagga aagaaagggt gagggagagg
     3361 attgagaatt tgtaggggcg aagccctgtc ttcgcccatg atttaagcaa accgcacctt
     3421 tgtcctcttc ttaggtatga accatcacgg tttataccta agaaacgaga ggattgagga
     3481 gaggttgaaa gatttgtagg cgacccaggc gggtcgcctg cggtct
//
LOCUS       DASWLN010000179         3087 bp    DNA     linear   ENV 20-FEB-2024
DEFINITION  MAG TPA_asm: Candidatus Zixiibacteriota bacterium isolate
            SMAG_U2949 k141_1128883, whole genome shotgun sequence.
ACCESSION   DASWLN010000179 DASWLN010000000
VERSION     DASWLN010000179.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37960594
            Sequence Read Archive: SRR10376091
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Candidatus Zixiibacteriota bacterium (soil metagenome)
  ORGANISM  Candidatus Zixiibacteriota bacterium
            Bacteria.
REFERENCE   1  (bases 1 to 3087)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 3087)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (26-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/12/2024 08:28:26
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 1,440
            CDSs (total)                      :: 1,416
            Genes (coding)                    :: 1,412
            CDSs (with protein)               :: 1,412
            Genes (RNA)                       :: 24
            rRNAs                             :: 1 (16S)
            partial rRNAs                     :: 1 (16S)
            tRNAs                             :: 21
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 4
            CDSs (without protein)            :: 4
            Pseudo Genes (ambiguous residues) :: 0 of 4
            Pseudo Genes (frameshifted)       :: 0 of 4
            Pseudo Genes (incomplete)         :: 3 of 4
            Pseudo Genes (internal stop)      :: 1 of 4
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..3087
                     /organism="Candidatus Zixiibacteriota bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_1128883"
                     /isolate="SMAG_U2949"
                     /isolation_source="soil"
                     /db_xref="taxon:2053527"
                     /environmental_sample
                     /country="USA: Oregon"
                     /lat_lon="42.99 N 123.63 W"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            <1..224
                     /locus_tag="VGB16_06580"
     CDS             <1..224
                     /locus_tag="VGB16_06580"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=3
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEX9917387.1"
                     /translation="RTPPTGRGFKTQTGIPKQVRDDKDRNKFKMTETLKSNPVVMLNS
                     FQHLWPDARAGLFEHLICFLECEPLSLLY"
     gene            436..1779
                     /locus_tag="VGB16_06585"
     CDS             436..1779
                     /locus_tag="VGB16_06585"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_018032231.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="acetyl ornithine aminotransferase family
                     protein"
                     /protein_id="HEX9917388.1"
                     /translation="MAMKNYPKIITKLPGPKSKKLINKDEKFVSQCYTRAYPTVIDHA
                     KGVYIWDVDGNCFIDFHSGIGVCSTGNCHPQVVEAIKRQAEKAVHIASADFYHKPVGD
                     LAMKMVEITPGGKDKRAFFANSGTESVEAAIKLARYNTRKPRMIAFIGAFHGRTMGAL
                     SLTCSKGTQRRHFAPMLPEVTHVPYAYCYRCPYNLTYGKCDMACVKFIDEVILQKVAP
                     AEDVAAIVVEPIQGEGGYIVPPKEFHPMLKRICEKYGILFVMDEVQAGMGRTGKMFAI
                     EHWNVVPDILVAAKAIASGLPLGACVASKKLMSWEPGAHSTTFGGNPIACAAALKTIE
                     LLENGLIKNAEKVGNFIMKRLAKMKAKYEIIGDVRGKGLMIGIELVKSKKTKEPAGEY
                     AEKAMQQCFRNGLMLLTCGQSGVRFIPPLIIDEETADRGMDIFEKVITKINHKNK"
     gene            1786..2976
                     /gene="sucC"
                     /locus_tag="VGB16_06590"
     CDS             1786..2976
                     /gene="sucC"
                     /locus_tag="VGB16_06590"
                     /inference="COORDINATES: protein motif:HMM:TIGR01016.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004774 - succinate-CoA ligase activity
                     [Evidence IEA];
                     GO_function: GO:0004775 - succinate-CoA ligase
                     (ADP-forming) activity [Evidence IEA];
                     GO_function: GO:0004776 - succinate-CoA ligase
                     (GDP-forming) activity [Evidence IEA];
                     GO_process: GO:0006099 - tricarboxylic acid cycle
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="succinate-CoA ligase subunit beta"
                     /protein_id="HEX9917389.1"
                     /translation="MRLYEYEAKQIFSEFGILLPQEKLVTTPEEAEEFASQLGKPVVL
                     KVQILSGGRGKAGGIKFAGNPQEAKAKAIELFSGKIKGYPVNKILIGEKLNVKGEFYL
                     GITIDRINYKIVILTCAEGGVEIEEIAKKSPQKIKRIALDIDDRFYPFQAQALAKQLG
                     FSGSNLRSVGDIVLSLFALFKRYDAKLVEINPLILTSESRVIAADARMSIDDDALFRH
                     PELEKMGIQKRHEEGEMTEREKQAKEWEIPYLDLDGDIGMFPGGAGFGIMGNDFIHHF
                     GGSPANFMDSGGGPAPERLAKMLTLLDENPKVKVIFAARFGGISRCDDFAKGVVKFLK
                     EHGLSKPMVMRMTGNMWQEGVGIFQEAKKRSPKLFEKIEIHGIETPIEEIAKRAVELA
                     KSVK"
BASE COUNT          970 a          585 c          764 g          768 t
ORIGIN      
        1 aaagaacccc gcccacaggg cggggcttta aaacccaaac cgggatcccg aaacaagttc
       61 gggatgacaa agaccgaaac aagttcaaga tgacagaaac cttaaaatca aacccggttg
      121 tcatgctgaa ctcgtttcag catctctggc ctgacgccag ggcaggcttg tttgagcatc
      181 tgatctgttt tttggagtgc gaacctttaa gtttgctata ttaagataat ataaagctaa
      241 agctttatac tccagttcat ggaaacctga aggtttccgc cacattaaac ttaactgatt
      301 tttaaataaa atgttccata cagttcgaag aatcctgcat agattctatc ttaagggaca
      361 gacctaatct ttccttataa ttttttattt aagatgactc ggaaaactta acctgattat
      421 aaaaaggaga tagaaatggc tatgaagaat tatccgaaaa tcataaccaa acttcccggc
      481 cccaagagca aaaagctaat aaacaaagat gaaaagttcg tttctcaatg ttatacccgc
      541 gcctatccta cggtcataga ccacgcaaaa ggggtttata tctgggatgt ggatggaaat
      601 tgttttatcg atttccattc cggcataggg gtttgctcaa ctggaaactg ccatccccag
      661 gtagtagaag caatcaaaag gcaagcggaa aaagcagtgc atatcgccag tgctgatttt
      721 tatcacaagc cggtaggcga tttggctatg aaaatggtag agataacacc tggaggaaaa
      781 gataaaagag ctttcttcgc caattccggc accgaatcag ttgaggcagc aatcaagctt
      841 gcccggtata acaccagaaa accacggatg atagctttca tcggcgcttt tcatggcagg
      901 accatgggag cgctctcgct gacctgcagt aaagggaccc agaggagaca ttttgcacct
      961 atgcttcccg aagtaactca tgttccttat gcctattgct atcgctgtcc ctataatctt
     1021 acctatggaa agtgcgatat ggcctgcgta aagttcatag acgaagtgat attacagaaa
     1081 gtagcaccgg ctgaagatgt ggcggcaatt gtggttgagc cgattcaggg agaaggcgga
     1141 tatatcgttc ctccaaaaga gtttcatcca atgctaaaaa ggatatgcga gaaatatggt
     1201 attcttttcg taatggatga agtgcaagca ggaatgggaa ggaccggaaa gatgtttgcc
     1261 atcgagcatt ggaatgttgt gccggatata ttggttgcag ctaaagccat tgcatctggt
     1321 ctgcctttag gagcctgcgt tgcctctaag aagctaatgt cctgggaacc gggcgctcat
     1381 tccaccacat ttggtggcaa tccgattgct tgtgccgcag cattaaagac catcgagctt
     1441 ttggagaatg gtttgattaa aaatgccgaa aaggtaggta attttataat gaagagatta
     1501 gccaaaatga aagccaagta tgagataatc ggtgatgtta gagggaaggg tttgatgatt
     1561 ggcatagagt tagtcaagag caagaaaacc aaggagccag caggggaata cgcagagaaa
     1621 gcaatgcagc agtgtttcag aaatggtcta atgcttctca cttgtggcca aagcggagtc
     1681 agatttatcc cacctctaat aatcgatgag gaaacagctg ataggggaat ggatatattc
     1741 gagaaggtta taaccaagat aaaccataag aataaataag gtcctatgcg gctttacgaa
     1801 tacgaagcca aacaaatctt ctccgagttt ggtatcctgc tcccgcagga aaaattagtg
     1861 acaactccgg aagaggcgga ggagtttgct tcccagttgg gcaaacctgt ggtcttgaag
     1921 gttcagattc tgtctggagg aagaggtaag gcaggaggca tcaaattcgc agggaatcct
     1981 caagaagcca aagccaaagc gattgagctt ttttccggca agataaaggg ttatccggtc
     2041 aataaaatct tgattggcga aaaactgaat gtgaaaggtg aattctattt aggcataact
     2101 atagaccgaa tcaactataa aatcgtcatc ttgacttgtg ctgaaggtgg agtggagatt
     2161 gaggagatag ctaaaaaatc tccacagaag ataaaaagaa tagccctgga tattgacgac
     2221 aggttttatc ctttccaagc ccaggctttg gctaaacagc tgggtttctc cggctcgaat
     2281 ctaagaagcg tgggggacat tgtcctctcg ctttttgctc ttttcaaaag atacgatgcc
     2341 aaattagtcg aaataaatcc cctaatcctg accagtgaga gcagggtcat tgccgcagat
     2401 gcccgaatga gcattgatga tgatgcgctt ttcagacatc cggaattgga gaaaatggga
     2461 atccagaaaa ggcacgaaga aggggagatg actgaaaggg aaaagcaggc aaaagaatgg
     2521 gagattcctt atctggattt ggatggggat atcgggatgt tccctggagg agccgggttc
     2581 ggcataatgg ggaacgattt cattcaccat tttggaggca gtcctgctaa ctttatggat
     2641 tcaggaggtg gtcctgctcc tgaaagatta gctaaaatgc tcactctttt ggatgaaaac
     2701 ccaaaggtga aagtcatctt tgcagctcgg tttggaggaa tatcccgatg tgatgacttt
     2761 gccaaagggg tagttaagtt cctaaaagag cacgggctgt ccaagccgat ggtgatgaga
     2821 atgacaggta atatgtggca ggaaggggtc ggtatatttc aggaggcaaa aaagcgaagc
     2881 ccgaagctct ttgagaaaat cgagattcac ggaatcgaaa cccctattga agagatagcc
     2941 aaaagagcag tggagttagc gaaatctgtt aaatgatact atgttggata caaacgactt
     3001 atattaataa tctaaagtaa tggttaaaga taaaatgaaa atagtggttg cattaccaga
     3061 atccagatac agctgtggcc agtttgc
//
LOCUS       DASWLN010000180         1534 bp    DNA     linear   ENV 20-FEB-2024
DEFINITION  MAG TPA_asm: Candidatus Zixiibacteriota bacterium isolate
            SMAG_U2949 k141_865832, whole genome shotgun sequence.
ACCESSION   DASWLN010000180 DASWLN010000000
VERSION     DASWLN010000180.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37960594
            Sequence Read Archive: SRR10376091
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Candidatus Zixiibacteriota bacterium (soil metagenome)
  ORGANISM  Candidatus Zixiibacteriota bacterium
            Bacteria.
REFERENCE   1  (bases 1 to 1534)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 1534)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (26-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/12/2024 08:28:26
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 1,440
            CDSs (total)                      :: 1,416
            Genes (coding)                    :: 1,412
            CDSs (with protein)               :: 1,412
            Genes (RNA)                       :: 24
            rRNAs                             :: 1 (16S)
            partial rRNAs                     :: 1 (16S)
            tRNAs                             :: 21
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 4
            CDSs (without protein)            :: 4
            Pseudo Genes (ambiguous residues) :: 0 of 4
            Pseudo Genes (frameshifted)       :: 0 of 4
            Pseudo Genes (incomplete)         :: 3 of 4
            Pseudo Genes (internal stop)      :: 1 of 4
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..1534
                     /organism="Candidatus Zixiibacteriota bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_865832"
                     /isolate="SMAG_U2949"
                     /isolation_source="soil"
                     /db_xref="taxon:2053527"
                     /environmental_sample
                     /country="USA: Oregon"
                     /lat_lon="42.99 N 123.63 W"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            <1..483
                     /gene="leuD"
                     /locus_tag="VGB16_06595"
     CDS             <1..483
                     /gene="leuD"
                     /locus_tag="VGB16_06595"
                     /EC_number="4.2.1.33"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_012870107.1"
                     /note="catalyzes the isomerization between
                     2-isopropylmalate and 3-isopropylmalate in leucine
                     biosynthesis; Derived by automated computational analysis
                     using gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="3-isopropylmalate dehydratase small subunit"
                     /protein_id="HEX9917390.1"
                     /translation="KAWVVGDDIDTDQIYHGQYLPLTDPGEMAKHAMEFVPGMENFVK
                     EVKPGDILVAGKNFGCGSSREHAVICLMENKVSCIIADSFARIFYRNAINLGFPVLEC
                     PGISSSIKNGQTIEVDLASGKIKTLDNDRKLKGVPLSGLEMEITDKKGLLNYLKSEKC
                     "
     gene            477..>1534
                     /gene="rlmD"
                     /locus_tag="VGB16_06600"
     CDS             477..>1534
                     /gene="rlmD"
                     /locus_tag="VGB16_06600"
                     /EC_number="2.1.1.190"
                     /inference="COORDINATES: protein motif:HMM:TIGR00479.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008649 - rRNA methyltransferase activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="23S rRNA (uracil(1939)-C(5))-methyltransferase
                     RlmD"
                     /protein_id="HEX9917391.1"
                     /translation="MLKKGETVELKVEDLAYQGKAIAKLKSLVIFLNRGIPGDVVRAK
                     ITKIKPNYLEAEVEKIIDKSCLRIEPACQHFGICGGCRLQDLKYEEQLKFKTKQVKES
                     LKHIAGLENAFVEETLPSPDIFFYRNKMEFSFGTGEKAELILGLHHLGKYDQNFDLVK
                     CFLQSEESNQVVRWVGDFAKEEGLVPYHVKTHQGYLRYLMIREGKFTQQMMVNLVTYL
                     GEFGVADKYAFGLRQKFPRIVSIVHNINSRLANIALGQEQAVLYGKDTINEKIGEFEF
                     EISSNSFFQTNSKQVGRLYQLVLESAGLRGDEVVWDLYSGCGGISIILSPFVKKVIGV
                     ESSPEANLDAQRNSNLNG"
BASE COUNT          476 a          283 c          378 g          397 t
ORIGIN      
        1 aaagcctggg tagtagggga tgatatcgat actgaccaga tatatcatgg acaatatctt
       61 cctttgactg accctggtga gatggcaaaa cacgctatgg aatttgtgcc ggggatggag
      121 aattttgtca aggaagttaa acccggggat attcttgttg ccggaaagaa ctttggatgc
      181 ggctcctcac gcgaacatgc agtgatatgc cttatggaaa ataaagtcag ttgtataata
      241 gcggactcat ttgctcgaat attctatcgc aatgctataa atttgggatt tcctgtgttg
      301 gagtgtccgg gcatttccag ttcgattaaa aacggccaga caattgaagt tgacctcgcc
      361 agtggaaaga taaaaactct tgataatgac aggaaattga aaggggtgcc tctttccgga
      421 ttggaaatgg aaatcacaga caaaaaaggg cttctgaact acctcaagag tgaaaaatgt
      481 tgaaaaaggg agaaaccgta gagctgaaag tagaagattt agcttatcag ggtaaagcga
      541 tagccaaact taaaagtttg gttatttttt taaacagggg gattccgggt gatgtggttc
      601 gtgccaaaat cactaagata aaaccaaact acctagaagc tgaagtagaa aagataatag
      661 acaaatcctg tctccgtata gagccagcct gccagcattt tgggatatgc gggggatgtc
      721 gtctgcaaga cctgaaatat gaggaacagc tcaagttcaa aaccaagcaa gtaaaggaaa
      781 gtttgaaaca tatagctgga ttggagaacg cttttgttga ggaaacgctt ccttctccgg
      841 acattttttt ctacaggaat aagatggagt tttcgtttgg tacaggtgag aaagctgaac
      901 taattttggg tctgcaccac ttgggcaagt atgaccagaa ctttgactta gtgaaatgct
      961 tcctgcaatc tgaagaatcc aatcaggtag ttagatgggt aggcgatttt gccaaagaag
     1021 aagggcttgt accctaccat gttaaaaccc accaagggta cttgcgctat ttaatgataa
     1081 gggaagggaa attcacccaa cagatgatgg tcaatctggt gacctactta ggcgaatttg
     1141 gagttgccga caagtatgct tttggtctaa ggcagaagtt cccgcgaatc gtaagcatag
     1201 tgcacaacat aaactcacgt ttagccaata tcgccctggg tcaggaacaa gctgtgcttt
     1261 acggtaaaga taccattaac gagaagatag gtgaatttga gtttgagatt tcaagtaact
     1321 ccttcttcca gaccaacagc aagcaggtgg gaagactcta tcagcttgtt ttagaatcag
     1381 ctggcttaag aggagatgag gtagtttggg acctttattc cggctgcgga ggcatctcca
     1441 tcatcctctc gccctttgtc aagaaagtca ttggtgtgga atcatcaccc gaagcaaatc
     1501 tggatgccca aaggaactcc aacctaaatg gaat
//
LOCUS       DASWLN010000181         3327 bp    DNA     linear   ENV 20-FEB-2024
DEFINITION  MAG TPA_asm: Candidatus Zixiibacteriota bacterium isolate
            SMAG_U2949 k141_242321, whole genome shotgun sequence.
ACCESSION   DASWLN010000181 DASWLN010000000
VERSION     DASWLN010000181.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37960594
            Sequence Read Archive: SRR10376091
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Candidatus Zixiibacteriota bacterium (soil metagenome)
  ORGANISM  Candidatus Zixiibacteriota bacterium
            Bacteria.
REFERENCE   1  (bases 1 to 3327)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 3327)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (26-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/12/2024 08:28:26
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 1,440
            CDSs (total)                      :: 1,416
            Genes (coding)                    :: 1,412
            CDSs (with protein)               :: 1,412
            Genes (RNA)                       :: 24
            rRNAs                             :: 1 (16S)
            partial rRNAs                     :: 1 (16S)
            tRNAs                             :: 21
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 4
            CDSs (without protein)            :: 4
            Pseudo Genes (ambiguous residues) :: 0 of 4
            Pseudo Genes (frameshifted)       :: 0 of 4
            Pseudo Genes (incomplete)         :: 3 of 4
            Pseudo Genes (internal stop)      :: 1 of 4
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..3327
                     /organism="Candidatus Zixiibacteriota bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_242321"
                     /isolate="SMAG_U2949"
                     /isolation_source="soil"
                     /db_xref="taxon:2053527"
                     /environmental_sample
                     /country="USA: Oregon"
                     /lat_lon="42.99 N 123.63 W"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            <1..528
                     /locus_tag="VGB16_06605"
     CDS             <1..528
                     /locus_tag="VGB16_06605"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEX9917392.1"
                     /translation="PYQPYSINQTLGCYFGFDVTPKIVILGGIDLNRFQDKYTATSEG
                     DVYESELSMTQFIPNLGVKFYFKPRASGDFSPYIFGGFFKGFASSNYEYTGGSAEYLK
                     FVEEIIKELNSPFGFIPAFGVEYYFSDNFGLGGEMGWRFSFSKADFDWADGEKDEYKY
                     NAISHYFGLTVNFHF"
     gene            complement(710..1876)
                     /locus_tag="VGB16_06610"
     CDS             complement(710..1876)
                     /locus_tag="VGB16_06610"
                     /inference="COORDINATES: protein motif:HMM:NF033577.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004803 - transposase activity [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="IS481 family transposase"
                     /protein_id="HEX9917393.1"
                     /translation="MGNTLKHVIFLVRNQEVEMPWDNKTIFEQRLLMLQKLFKEEAPV
                     TRISREFGVSRKTFYKFKQRFEAEGVIGLKDRSRAPHQRPRKISSLVEETIVELRCLH
                     PDFGPVRLQAELRNLGLDPPSHQGIQNVLVRHHLNGWNATQKREYRRFERSSPNELWQ
                     LDTKGFFIIKDWGKVFPLNVLDDHSRFCIACQPHPFERTAYILDTLEKSFALYGKPQY
                     LMTDNGTWFAHRSGALTRVQMFLKDLEIGHIHTGIRRPTTIGKVERFHRTLNEELIKK
                     FRFKDLEDAKEAFEAYRYYYNYQRPHQALGNLPPISRYKFVQRQYPEPPVPVFYPEGS
                     LLRKVRGNGCIIFNKTEIYLSRILIGKVVKIEKNKNSLEVYHDKNLIKYVTYDS"
     gene            2452..2778
                     /locus_tag="VGB16_06615"
     CDS             2452..2778
                     /locus_tag="VGB16_06615"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_012373770.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="RNA-binding protein"
                     /protein_id="HEX9917394.1"
                     /translation="MSNNKLYVGNLSYMTGEEKLREAFSAYGTVTTITIIEGKGFGFV
                     EMETGEAAQAAKEALDGKELDRRNIKVDEARPRPKRSGGGGGGYGGGGSRGGGDRDFK
                     RRRPRY"
     gene            2987..3292
                     /locus_tag="VGB16_06620"
     CDS             2987..3292
                     /locus_tag="VGB16_06620"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEX9917395.1"
                     /translation="MPFSKEKWIEIARDRGLEEVGVKLIEKTGKCPHAEGEVFIYVHP
                     NCRPEGLCGVAAIAIEPFVQRCSARVPSWEKDDSNIYRIHCPSKKGTIWEIGRLKDK"
BASE COUNT          967 a          671 c          751 g          938 t
ORIGIN      
        1 ccttatcaac catattcgat caatcagaca ttaggctgtt atttcggctt tgatgtcact
       61 ccgaagattg ttatcctggg tggtatagat ttaaatcgct ttcaggataa atatacagcg
      121 acatcagaag gagatgttta tgagtccgag ctgtccatga ctcagttcat accgaacctt
      181 ggtgtgaaat tttacttcaa gcccagagcc agtggagatt tttcacccta catattcggc
      241 gggttcttca agggcttcgc ttcttcgaat tatgaatata ctgggggctc tgctgaatac
      301 cttaagtttg ttgaagaaat aatcaaggag ttgaatagcc cgtttggttt tatacctgcc
      361 tttggagtgg agtattactt ctccgataac tttggacttg gcggcgagat gggttggaga
      421 tttagctttt cgaaagcaga tttcgattgg gctgatggtg aaaaggatga atataaatac
      481 aacgccattt cccattactt tggtctgact gttaatttcc acttctaatt cgaccgggat
      541 gtagtttacc aaaaaagggc gaagaaattc gccctttttg tttgtttttt tgtagggaca
      601 gaacagtgtt ctgtccgaat gaaagacgta gagaggcatc gcatgcgtct ccaagtaatt
      661 gtagcgtcgg ggtttattgt actatcaagt agcataggtt acagatctat caactgtcat
      721 aggtaacata cttgatcaag tttttatcat gatagacctc caaactgttt ttgttctttt
      781 ctatctttac tactttacca atcaggattc tggataagta gatttcagtt ttattgaaaa
      841 taatacagcc attccctcgt actttcctta acaaacttcc ttccgggtaa aagacaggca
      901 caggtggttc tggatactgt cgttgcacaa acttatatcg agatataggt ggaagattcc
      961 ccaaagcctg atgtggtctt tgataattat aataatagcg ataagcctca aaggcctcct
     1021 tggcgtcttc taagtcctta aagcgaaact tcttaatcag ctcttcattc aaagtgcgat
     1081 gaaacctttc aactttgccg atggtggtag gacgacgtat tccagtatgg atatgaccaa
     1141 tctctaaatc tttaagaaac atttgcaccc gggttaaggc tccagacctg tgagcaaacc
     1201 aagttccgtt atctgtcata agatattgag gtttcccgta aagagcaaaa gatttttcta
     1261 aagtgtctaa aatatatgcg gttctttcaa agggatgagg ttgacaggcg atacagaaac
     1321 gagaatgatc atccaggaca ttcaaaggaa aaaccttacc ccaatctttg ataatgaaaa
     1381 atcctttagt gtctaactgc cagagttcat taggagagga gcgttcaaat cggcggtatt
     1441 ctcttttctg tgtagcattc cagccattaa gatgatgacg tactaaaaca ttttgaattc
     1501 cctgatggga aggaggatct aaacctaagt tccttagttc tgcctgtaga cgaacaggtc
     1561 caaagtcggg atgaagacaa cggagttcca ctattgtctc ctccactaga gacgatatct
     1621 ttctgggtct ctgatgaggt gcacgacttc gatcctttaa acctataact ccttctgctt
     1681 caaagcgctg cttaaactta tagaaggttt tgcgagaaac tccaaactct cttgagatcc
     1741 tagttactgg agcttcctct ttgaataatt tttgtaacat gagtaacctc tgttcaaata
     1801 tggtcttatt atcccatggc atctctacct cctggtttct aaccaggaat ataacatgtt
     1861 ttaaggtgtt acccatgtta gttgatactt ctgttaccta tgtaggttga taatacagtt
     1921 tatccctgat gtttgttcat tttcgtcggg cataaaaccc gacgttacat ataagacctc
     1981 accctgtccc ccttcttagt agagggacag gatagaatcg aataaaattt tcagtgcttg
     2041 ccatctgcct gatggggact aatctggcaa gtcaactttc tcttatcggt agggtggcac
     2101 cctcaaactc gtttgagggt ggtgcaggag gtttctgcgc ttgcctttca taacatctcc
     2161 aaaacaagtt tgggatggca aaccgcatag ccaccacagg atttgaacaa aaaaggctcc
     2221 caaccatttt agtggtgtag gagacagcaa ttaaaataaa aattttactt gacttttgtg
     2281 cttaaacatc gtagatattt attgtcaacc attagggcgc ggcttggcgc tgacccggct
     2341 gcaactgcag cttagaatgt aagaagggca aaaatccctt ttccatctgt acggactccg
     2401 ctcaaagcac aaaccctttt caaaaacact ttaactccaa aggaggagta agtgagtaac
     2461 aacaagctat acgtaggcaa cctgagctac atgactggtg aggagaagct cagagaagct
     2521 ttctcagcct acggaactgt aaccaccatc accataatcg aaggaaaagg attcgggttt
     2581 gtggagatgg aaaccggtga ggcagcccag gccgccaaag aggctctgga tgggaaagaa
     2641 ctggatcgtc gtaacatcaa ggtggacgaa gcccggccca ggccaaaaag aagcggcggc
     2701 ggtggcggtg gatatggagg agggggtagc agaggaggag gagacaggga ttttaaaaga
     2761 agacgaccca gatattagct ctgattagaa aagggtcggt ctacccagag tagaccgacc
     2821 cttattgttt actgagtact gtagtattgt agttgcccga ttcatcgacc aagagatagc
     2881 ccgatagatt aacaactgca gttcagatag gtcggggaat ataacgacac caatagaacc
     2941 tcaccctaac cctctcctta taaaggagag ggaaaaggag ttagatatgc cattttcaaa
     3001 agaaaaatgg atcgagattg ccagagacag aggattggaa gaagtagggg ttaaattaat
     3061 tgagaaaaca ggtaaatgcc ctcatgcaga aggggaagtt tttatatatg tccatccaaa
     3121 ttgcagacct gaaggacttt gcggagtggc tgccattgca atagagcctt ttgttcagcg
     3181 ctgttcagcc agagtcccat cctgggagaa agacgactcg aatatatatc gcattcactg
     3241 tccttcaaaa aaagggacca tctgggagat agggagatta aaagataaat aggctctttc
     3301 tgctcgggca ggggtataac acctatc
//
LOCUS       DASWLN010000182         5592 bp    DNA     linear   ENV 20-FEB-2024
DEFINITION  MAG TPA_asm: Candidatus Zixiibacteriota bacterium isolate
            SMAG_U2949 k141_244722, whole genome shotgun sequence.
ACCESSION   DASWLN010000182 DASWLN010000000
VERSION     DASWLN010000182.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37960594
            Sequence Read Archive: SRR10376091
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Candidatus Zixiibacteriota bacterium (soil metagenome)
  ORGANISM  Candidatus Zixiibacteriota bacterium
            Bacteria.
REFERENCE   1  (bases 1 to 5592)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 5592)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (26-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/12/2024 08:28:26
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 1,440
            CDSs (total)                      :: 1,416
            Genes (coding)                    :: 1,412
            CDSs (with protein)               :: 1,412
            Genes (RNA)                       :: 24
            rRNAs                             :: 1 (16S)
            partial rRNAs                     :: 1 (16S)
            tRNAs                             :: 21
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 4
            CDSs (without protein)            :: 4
            Pseudo Genes (ambiguous residues) :: 0 of 4
            Pseudo Genes (frameshifted)       :: 0 of 4
            Pseudo Genes (incomplete)         :: 3 of 4
            Pseudo Genes (internal stop)      :: 1 of 4
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..5592
                     /organism="Candidatus Zixiibacteriota bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_244722"
                     /isolate="SMAG_U2949"
                     /isolation_source="soil"
                     /db_xref="taxon:2053527"
                     /environmental_sample
                     /country="USA: Oregon"
                     /lat_lon="42.99 N 123.63 W"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            <1..217
                     /locus_tag="VGB16_06625"
     CDS             <1..217
                     /locus_tag="VGB16_06625"
                     /inference="COORDINATES: protein motif:HMM:NF012790.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=2
                     /transl_table=11
                     /product="rhodanese-like domain-containing protein"
                     /protein_id="HEX9917396.1"
                     /translation="LPFEEFDKYYYSVKNKLPLDRTLIIYCSGTECEASLFLARLLRG
                     LGYRNLKVFFGGWEGWQKANLPIATKE"
     gene            228..674
                     /locus_tag="VGB16_06630"
     CDS             228..674
                     /locus_tag="VGB16_06630"
                     /inference="COORDINATES: protein motif:HMM:NF018942.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0016020 - membrane [Evidence IEA];
                     GO_process: GO:0030416 - methylamine metabolic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="MauE/DoxX family redox-associated membrane
                     protein"
                     /protein_id="HEX9917397.1"
                     /translation="MKRMLSNKYLVFVFRLIVGGIFIYASIDKITHPEQFARIVYNYK
                     ILPHFTINFFAIILPWLELICGLFLVFGIMTRSSALVISILLLLFIIALSVNLFRGVD
                     LSCGCFTTNLANKERISSIIWRDLVLVLLSLEILFFTGTQLRDKTI"
     gene            complement(908..1945)
                     /gene="mtnA"
                     /locus_tag="VGB16_06635"
     CDS             complement(908..1945)
                     /gene="mtnA"
                     /locus_tag="VGB16_06635"
                     /EC_number="5.3.1.23"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011734455.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0046523 -
                     S-methyl-5-thioribose-1-phosphate isomerase activity
                     [Evidence IEA];
                     GO_process: GO:0019509 - L-methionine salvage from
                     methylthioadenosine [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="S-methyl-5-thioribose-1-phosphate isomerase"
                     /protein_id="HEX9917398.1"
                     /translation="MKFKTLDWKDNKLILLDQTKLPLKEVYLKLDNHKKVAQAIKSLK
                     VRGAPAIGVAAAYGVVLGMQNRKYKSIQEYKTRLEKVISDLKNTRPTAFNLFWALGRM
                     ANLFKKNQNKTVSKINPVTGGINQLLLEEAKKIHREDQLMCRKIGQNGAKLLKNGYTI
                     LTHCNAGALATSGMGTALSIIYIAKKQGKKMKVFVDETRPVLQGARLTTWELLQEGID
                     TTLICDNMAAFLMKQKKLDCVIVGADRIARNGDVANKIGTYNLAVLAEVHKIPFYVAA
                     PQSTFDNNISDGEKIKIEERSPKEVIFCGNKRIAPKNVKVYSPAFDVTPSELVSAIIT
                     DKGIIKGKRKD"
     gene            complement(2355..2867)
                     /locus_tag="VGB16_06640"
     CDS             complement(2355..2867)
                     /locus_tag="VGB16_06640"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEX9917399.1"
                     /translation="MRKLLFVILFLVSPSIAFPQPIEFKLSFWKGGSYYIQEGKTYGM
                     GWYGRNVKKIISSSPEAIREVNKYYTYQTIAYALGFSGAFMINYALFDHEHLGSSEEY
                     GLIIGGVGLIGINVGLGYMGFSHLKKAAEMYNSSLKARETSAKQDPKTVRVALGYNED
                     YYGITLSYNF"
     gene            complement(3217..4221)
                     /locus_tag="VGB16_06645"
     CDS             complement(3217..4221)
                     /locus_tag="VGB16_06645"
                     /inference="COORDINATES: protein motif:HMM:NF012379.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0000166 - nucleotide binding [Evidence
                     IEA];
                     GO_function: GO:0004812 - aminoacyl-tRNA ligase activity
                     [Evidence IEA];
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_process: GO:0043039 - tRNA aminoacylation [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="amino acid--tRNA ligase-related protein"
                     /protein_id="HEX9917400.1"
                     /translation="MDVSSTKLISQTLKSSRCYLEEKGYVEVTVPRVVRASGACENVN
                     TLFEVSVDGDFSWFNRNKGYLAQTGQLYLESMVPYLGKVYCSGSSFRAEPRADNRHLI
                     EFQLLEIEFAGNFDELLKELQGIVQRMASDLLKHPNPEKEFGLEEKDLKRLENSLVDF
                     PRVTYDEAIELLNQLGEEISWGQDITSQMERKIVDYFREVPVFITRFPDPCWDFGKEI
                     EVEKFFNMLPDPENPGRVLSCDLIMPYGGEAVGAAARVHKGDILAQRLSHSRMFKRLL
                     ERGGGWGDFAWYLDRISENGCVPHVGGGFGLARVIQFIKGSSDIRDCVSFVSNKECLI
                     "
     gene            complement(4258..5571)
                     /locus_tag="VGB16_06650"
     CDS             complement(4258..5571)
                     /locus_tag="VGB16_06650"
                     /EC_number="3.5.2.3"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011938750.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004151 - dihydroorotase activity
                     [Evidence IEA];
                     GO_function: GO:0008270 - zinc ion binding [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="dihydroorotase"
                     /protein_id="HEX9917401.1"
                     /translation="MKKKNNKDKFDLVISGGRVIDPASDFDKMADIYIQDGKVVKIES
                     DKRDKKEEVIVLQIVDARGKIVCPGLIDMHTHLREPGREDEETIYTGSMAAVAGGFTS
                     ICCMPNTFPPIDNQETVKFILEKAKFAKCRIFPIACITKEQKGEEITEVCDLVQAGAV
                     ALSDDGLPVSNSQVMRNILDYALMFGIPIISHCEDLTLSGDGVMNESFISSLLGMKGI
                     PSVAEEIAVGRDIKLAEYTKGKLHIAHVSTGGSVHLVREAKRRKVNISCEVTPHHFSL
                     TDELVKTFNTNLKTNPPLRTKKDVLAIKRGLKDGTIDVIATDHAPHSIEEKDVEFDAA
                     PNGMIGLETALSLVISELVDKNVLSWKEAIAKLTVNPAKILNLDLGKIKVGSEADITI
                     IDPKVNWVVSPEKLSSKSKNSPFIGKRLKGRAWCTIVGGKIVYQL"
BASE COUNT         1473 a         1307 c         1025 g         1787 t
ORIGIN      
        1 tcttcccttt gaggagtttg acaaatatta ttattcagtg aaaaataaac tccctttaga
       61 tagaacttta attatctact gtagcggaac tgaatgtgaa gcaagtctat ttctggctcg
      121 acttttgaga ggtctcgggt atcgtaattt gaaagttttc ttcggtggat gggaagggtg
      181 gcaaaaagca aacctgccaa ttgcgactaa ggaatgatat gtctcttatg aaaaggatgt
      241 taagcaataa atatctggtc tttgtattca gattaatagt cggtggaatt tttatctatg
      301 ccagtataga taagataact catccggagc agtttgcccg catcgtttac aactataaga
      361 tacttcctca ttttaccatc aatttctttg ccatcattct tccctggtta gagcttattt
      421 gtggactttt tctcgttttc ggaattatga cccgaagctc ggctttggtc atctccattc
      481 ttctgctctt attcatcatc gctctttcag ttaatctctt tagaggggtg gatttgagtt
      541 gtggctgttt tactactaat ttagctaaca aagaacgcat atcttctatt atatggagag
      601 atttggtatt ggttctattg agcttggaga ttttattctt tactggaacg caattgaggg
      661 ataagactat ctagtgaaac attcccaaaa tgatatcaat ctgtcattgc gaggaacgaa
      721 gtgacgaagc aatctcatcg gtttttaatg attggagatt gcttcgtccc ctacggggac
      781 tcgcaatgac aaattattat agcacctctt agggaaaaat ttaaagaata tttcaaggga
      841 ataaggtctg cctacataaa aacggtaatg cgtaggggcg gagcttgtct ccgcccaata
      901 agagaaatta gtcttttctc ttgcctttta ttatcccctt atcagttata attgcactta
      961 ccagctcgga tggagtaacg tcaaaagcag gcgaatagac ttttacattt ttgggtgcaa
     1021 tcctcttgtt tccacaaaag ataacttctt ttggagaacg ctcttctatc tttatctttt
     1081 caccatcgga gatattatta tcaaaggttg attgcggggc tgccacgtaa aatggaattt
     1141 tatgaacttc ggctaaaacc gccagattat aggtgccgat tttatttgcc acatccccat
     1201 ttctggcaat tctatctgca cccactatta cacagtcaag ttttttctgt ttcatcaaaa
     1261 aagcagccat attatcacaa attaaggtgg tatcaatccc ctcctgcaag agttcccagg
     1321 tggttaacct tgcaccctgc aagaccggtc tggtctcatc cacaaagact ttcattttct
     1381 tgccctgctt ttttgcaata tagatgatgc tcaaagctgt ccccattcca ctggtggcta
     1441 atgctccagc attacagtgg gtcaaaattg tatatccatt tttcaatagc ttggctccat
     1501 tttgaccgat ttttctgcac atcaattggt cttctcgatg aatctttttc gcttcttcta
     1561 aaagcaactg atttatcccg cctgtgacgg gatttatctt acttacggtc ttgttttgat
     1621 ttttcttaaa cagatttgcc attcttccca aagcccagaa aagattaaat gcagtcggtc
     1681 gagtgttttt taaatctgat attacctttt ctaatctggt tttatattct tgaatggatt
     1741 tgtatttacg attttgcatt cccaaaacca cgccataagc cgccgccacc ccaattgccg
     1801 gcgcacctct gacttttagg ctttttatgg cttgtgctac ttttttgtga ttgtctaact
     1861 tcaaatagac ttccttcaaa ggaagcttgg tctggtccag aaggatgagt ttattatctt
     1921 tccaatctaa agtcttaaat ttcatttatt ctcctatgat ttgaagctca tcttcctaca
     1981 catctaacta accccctatt gggctgtccg ataacctctt tttcgccgtt tctgtcattg
     2041 cgagcgacca cgatctatcg ggggagcgtg gcaatctctt tgtttatcta tcgcttacga
     2101 gattgcttcg tcgtctcgca aaatgctgaa ctcctcgcaa tgacatttct ttggaaagcc
     2161 tcactgcctt tttctcccct gataagggga acttaaaggg gttaatgatt cccaattacg
     2221 aaaaatgtct tctataaaat ccccctttat ccccctttgt caaaggggga agttttatat
     2281 tcccctcttt gtaaaagagg ggttagggga gatttttgtt cctatttact tgtcaaaagc
     2341 atagaactct tgacctagaa attataagat agagtaatcc cgtagtaatc ctcattatat
     2401 ccaagagcaa ctcttaccgt tttggggtct tgtttggctg aagtttccct agctttcagt
     2461 gaactattat acatctcagc agcttttttc aagtgggaaa accccatata tcctaagcct
     2521 acattaatac ctattagacc tacgcctcca ataattaacc catactcttc actcgaaccc
     2581 agatgctcgt ggtcaaacaa tgcgtaatta atcatgaaag ctccactaaa tcctaaagcg
     2641 tatgcaatgg tttgatatgt ataatactta ttcacttctc ttatcgcttc aggagaagaa
     2701 gagataattt ttttcacgtt ccttccatac catcccattc cataggtctt gccctcttgt
     2761 atataatatg aacctccctt ccaaaaactg agtttgaatt caattggctg aggaaaagca
     2821 atgcttggtg aaactaaaaa caaaattaca aatagaagtt tcctcatttt taagctccta
     2881 ttaacttgtc tattgagtaa gtggtaaact ctattttcaa ttctgatata tttattcttt
     2941 tacttctgta gaaaccgggt aagtaatggc tgcgccatta cttttcttgt ttttttacgc
     3001 tcttaggcgg atgttattca tccgccgtct cttctagctt gctccttgtg gatgataaca
     3061 tccacaagga gcaagggaac ggtgcaatcg ttccctaccc gctaaataat ctcgttgtga
     3121 agaaatgtca agctggaaaa cagaaaaaac aaagaaggct ttttgaggaa agtctattcc
     3181 ctgtggaaaa attcgtagaa ttaaagaact atctttctaa atcaggcact ctttattcga
     3241 cacgaaggag acgcaatccc gaatatcaga gctccctttg atgaactgga tcactcgggc
     3301 taagccgaac ccgccaccca cgtgtggcac gcagccgttc tcgctgattc tgtccagata
     3361 ccaggcaaaa tcaccccatc ctccacccct ctccagaagc cgcttgaaca ttctcgaatg
     3421 ggaaagtctc tgggctagta tgtcaccctt atgcactcgt gctgccgctc ctactgcttc
     3481 accaccatag ggcataatca aatcacagct tagaaccctg cctggatttt ccgggtcagg
     3541 aagcatattg aagaacttct ccacctctat ctcttttccg aaatcccagc aagggtcagg
     3601 aaagcgggta atgaacaccg gcacttccct aaagtagtca actatctttc gctccatttg
     3661 ggaggtgata tcctgtcccc aggaaatctc ttctccaagc tggtttaaaa gttcgatagc
     3721 ttcatcgtaa gttactcgag ggaaatccac aagtgagttt tccagccgct tcaggtcctt
     3781 ttcttctaga ccaaactctt tttccgggtt aggatgtttt agcaaatccg atgccatccg
     3841 ctgaactatc ccttgaagct cttttaaaag ctcgtcgaag ttgccagcga attcaatctc
     3901 caggagctga aactcgatta gatgtcgatt gtccgctctc ggttcggccc tgaaggagga
     3961 gccgctgcag taaacttttc ccaaataagg gaccatagat tccagataga gttgcccggt
     4021 ctgcgccaaa taccccttgt tccgattgaa ccagctgaaa tccccatcca cgctcacctc
     4081 aaacagagta ttgacattct cacaggcccc actggctctt actactctgg gaactgtcac
     4141 ctccacatac cccttctctt ccagatagca tctggaggac tttagagttt gagagataag
     4201 cttagttgag cttacatcca ttcaacaacc tcctttctta aaagttaatg gttattatta
     4261 gagctgatac actatcttgc ctccgacaat ggtacaccag gctcttcctt tgagtctttt
     4321 tccaataaaa ggtgaattct ttgacttgga agagagtttt tccggactga ctacccagtt
     4381 aacttttggg tcgataatag ttatatctgc ttcgcttccc acctttatct tacccaaatc
     4441 caaattcaaa atcttagctg gatttacggt cagcttggcg atcgcctctt tccagctcaa
     4501 gacattttta tcgactagtt cggagatgac caaactcaaa gctgtttcca aacctatcat
     4561 cccattgggt gcagcatcaa actccacatc tttttcctca attgaatgag gtgcgtggtc
     4621 tgtggcgata acatcgatgg ttccatcctt cagccctctt tttatcgcca agacatcctt
     4681 cttcgttctt aaaggtggat tggttttcag attggtattg aaggttttga ccagctcatc
     4741 tgtcaggctg aaatgatggg gagtcacttc acagctgata tttacctttc ttcttttcgc
     4801 ctcgcggacc agatgcacag agccaccggt tgaaacgtgg gcgatatgaa gttttccttt
     4861 ggtatattca gccagtttta tatcccggcc aaccgctatc tcctctgcaa ctgaaggaat
     4921 ccctttcata cccaaaagag aagaaatgaa actctcattc atcaccccat caccagaaag
     4981 agtcaaatcc tcacaatgtg agataatggg aataccaaac atcaaagcat aatccagaat
     5041 attacgcata acttgagaat ttgaaaccgg caagccatca tctgaaaggg caactgcgcc
     5101 tgcttgaacc aaatcacata cttcagttat ctcctctcct ttttgttctt tggtgatgca
     5161 agcaattgga aaaattctgc atttggcaaa ctttgccttc tccagaatga acttgactgt
     5221 ctcctgattg tcgattggtg gaaaggtatt gggcatgcaa cagatggagg taaatccacc
     5281 cgccaccgct gccattgagc cggtatatat ggtctcttca tcctcacgtc ccggctcgcg
     5341 caggtgagta tgcatatcaa ttaagcctgg acagactatt ttcccccggg catcaactat
     5401 ctgtaaaaca attacctctt cttttttatc tcttttatcc gattctatct tgactacttt
     5461 tccgtcctgg atatagatgt cagccatctt gtcaaaatcg cttgccgggt caataaccct
     5521 gccaccagag attactaaat cgaacttatc tttattgttc ttctttttca acttttcttt
     5581 ctcctccact ca
//
LOCUS       DASWLN010000183        11063 bp    DNA     linear   ENV 20-FEB-2024
DEFINITION  MAG TPA_asm: Candidatus Zixiibacteriota bacterium isolate
            SMAG_U2949 k141_245722, whole genome shotgun sequence.
ACCESSION   DASWLN010000183 DASWLN010000000
VERSION     DASWLN010000183.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37960594
            Sequence Read Archive: SRR10376091
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Candidatus Zixiibacteriota bacterium (soil metagenome)
  ORGANISM  Candidatus Zixiibacteriota bacterium
            Bacteria.
REFERENCE   1  (bases 1 to 11063)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 11063)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (26-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/12/2024 08:28:26
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 1,440
            CDSs (total)                      :: 1,416
            Genes (coding)                    :: 1,412
            CDSs (with protein)               :: 1,412
            Genes (RNA)                       :: 24
            rRNAs                             :: 1 (16S)
            partial rRNAs                     :: 1 (16S)
            tRNAs                             :: 21
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 4
            CDSs (without protein)            :: 4
            Pseudo Genes (ambiguous residues) :: 0 of 4
            Pseudo Genes (frameshifted)       :: 0 of 4
            Pseudo Genes (incomplete)         :: 3 of 4
            Pseudo Genes (internal stop)      :: 1 of 4
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..11063
                     /organism="Candidatus Zixiibacteriota bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_245722"
                     /isolate="SMAG_U2949"
                     /isolation_source="soil"
                     /db_xref="taxon:2053527"
                     /environmental_sample
                     /country="USA: Oregon"
                     /lat_lon="42.99 N 123.63 W"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            271..726
                     /locus_tag="VGB16_06655"
     CDS             271..726
                     /locus_tag="VGB16_06655"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_020258353.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="dTDP-4-dehydrorhamnose 3,5-epimerase family
                     protein"
                     /protein_id="HEX9917402.1"
                     /translation="MIDGVKTKKLKVIPDERGRLMEILRSDDEFFSKFGQVYITTVYP
                     GVVKGWHYHKKQTDNIAVIVGMAKLVLYDNREDSPTKGETNEFFIGEHNPMLICVPKL
                     VMHGFKGISNCETMMLNCPTEVYNYKSPDEFRLPADTKEIPYNWAREDK"
     gene            1311..2318
                     /gene="rfbB"
                     /locus_tag="VGB16_06660"
     CDS             1311..2318
                     /gene="rfbB"
                     /locus_tag="VGB16_06660"
                     /EC_number="4.2.1.46"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016324757.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008460 - dTDP-glucose 4,6-dehydratase
                     activity [Evidence IEA];
                     GO_process: GO:0009243 - O antigen biosynthetic process
                     [Evidence IEA];
                     GO_process: GO:0019305 - dTDP-rhamnose biosynthetic
                     process [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="dTDP-glucose 4,6-dehydratase"
                     /protein_id="HEX9917403.1"
                     /translation="MKLLVTGGAGFIGSNFIRYILSRYPNYKIINLDKLTYAGNLDNL
                     SDSEKNKNYTFIKSDITDQKLVENILREKPDAILNFAAETHVDRSIYNPEDFVKTNIV
                     GTGVLMDAAIKYETPKFIQISTDEVYGSLGKKGTFTEESNLAPNSPYSASKAAADLLC
                     RSYFKTFDFPVIITRSCNNFGPYQFPEKLLPLFISNALSDNELPLYGDGLNIRDWIYV
                     LDNCRAVDIVLHKGKKGEIYNISSNNEMTNLEMTKLILKKLKKPESLIKFVKDRPGHD
                     RRYALDASKLRGDLGWKPKYSFEEGINKTVDWYLGNKAWWEKVKSGEYLNYYRKQYHN
                     R"
     gene            2621..3505
                     /gene="rfbD"
                     /locus_tag="VGB16_06665"
     CDS             2621..3505
                     /gene="rfbD"
                     /locus_tag="VGB16_06665"
                     /EC_number="1.1.1.133"
                     /inference="COORDINATES: protein motif:HMM:TIGR01214.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008831 - dTDP-4-dehydrorhamnose reductase
                     activity [Evidence IEA];
                     GO_process: GO:0009243 - O antigen biosynthetic process
                     [Evidence IEA];
                     GO_process: GO:0019305 - dTDP-rhamnose biosynthetic
                     process [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="dTDP-4-dehydrorhamnose reductase"
                     /protein_id="HEX9917404.1"
                     /translation="MKRVLVTGANGLLGQKIVEILSPAYKITSCDLGPEFFLSSKASY
                     HPLDIREKDKVVALVGSFKPSWIVNCASYTDVNGCETNQSLAWSVNVDGVKNIVEVCE
                     KLKIDLIHFSTDYVFDGKNGPYSEEDKTDPINFYGKTKLESEKIIEESHINFTIIRTN
                     VLYGCGEKVKSNFFLWVLENLKKNGKIKVVNDQYNNPTLAFNLAEIVKEAVDRNLFGL
                     YHIGGADWLSRFEFAQKVALSFGFDRDKIKPIQTSDLKQTAPRPLKGGLKIDKAKKVF
                     KTEIWGIEKSLGFLKGNC"
     gene            complement(4162..4929)
                     /locus_tag="VGB16_06670"
     CDS             complement(4162..4929)
                     /locus_tag="VGB16_06670"
                     /inference="COORDINATES: protein motif:HMM:TIGR02595.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="PEP-CTERM sorting domain-containing protein"
                     /protein_id="HEX9917405.1"
                     /translation="MKKLMLVLGLGVVLLFGGTAQASFIMTDLFNDFNTNLWMDYSHD
                     GGWATVESGYLALGTSNEAVRGGRGRAQSKFNLSGDFDTQVDFDLIDFNAFYSSASLY
                     VWAPDGSFGMKMARSYEDNGNNYYDANYQIGGNWSGDFFGLTSDMSGRFRIARLGNYL
                     GAYYWSGDTWVLATSTTLPEGISNNVRVFMETGNDGPYGSAPYVNVQHDNFYAHADGF
                     TNIDSKYLTAAPEPGTFILLGTGLLGLGTIVRMRRKR"
     gene            5701..8061
                     /locus_tag="VGB16_06675"
     CDS             5701..8061
                     /locus_tag="VGB16_06675"
                     /inference="COORDINATES: protein motif:HMM:NF014606.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="SLBB domain-containing protein"
                     /protein_id="HEX9917406.1"
                     /translation="MRKVASGLIVALVLSLFWVGFDLSAQTQEEKDKLYQEYQMKKGM
                     PQEGDIPKYQTPEIYEETKPVVELKKTATISVPSPVASASPGIPKAGGDLQPFGYNMF
                     NVTPSSFAPVTPTSVPPDYTLGPGDNIIVNLWGKVDLQLDLTVDREGKIFIPKAGDLT
                     VVGLTLDQFEKKLKEKLSTIYSGFRMSVMMGKIRLIKVFVFGEVTNPGGYTISSLSTL
                     FNALYEAGGPNQRGTLRNIKLVRNNQEIATIDIYDFLLKGYNKDDTKLASNDVIYVPV
                     VGSLVKIRGELKRPAIYELKGGEKILDLIELAGGFKTTAYLERVMVDRIAGQDKRMLL
                     DVNLLDQSKSNENNLELKDGDDVSVFSIFDLIENFVWIDGKVKHSGAFQRNPGMRVWD
                     LIGGGEQLQKDVYLLRADLIRTYPDERKEIIPVNLEKVLAGDSTENMILRDRDRLVVY
                     GYWDVNRKKFVNINGVVKKPGQYELYENIKLSDLIFLAGNPLKNAYLLRAEIAHLNPG
                     KPADISYVNLEDLYTDSENFIIKPDQNVDISLKEDDQIFIREIPEWSLQKIVTIEGEV
                     MFPGNYALEQEKEALYHLLQRAGGLTDKAFAKGAIFVRPSIIRDVERKHVGDVVVSSQ
                     LLERDSLGNILPPVTTPFNPDRLNRVIIDLPKTLRLNGGEDDIVLHDSDYVYIPRIPS
                     EVQVVGSVGASSSIKFYDGKSVKYYIDRAGGYTRNSDKGQTRLIGADGRVISGGSLRG
                     REVELGDVIVVPQKIAKHKDWMKLMANTVAVLSGLATTIFVIDRLK"
     gene            8578..9696
                     /locus_tag="VGB16_06680"
     CDS             8578..9696
                     /locus_tag="VGB16_06680"
                     /inference="COORDINATES: protein motif:HMM:NF014732.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="GNVR domain-containing protein"
                     /protein_id="HEX9917407.1"
                     /translation="MSEIQIRENRILAEDLKPNSTQEILVLFSSLVKKRKWIYLLVGV
                     TVLATTIFCLFLPNKYTAKAVILPSGGTTDKLGGLKEFTGLMGDFSLGVSSSNLENSS
                     FLYPDILKSRLLSEAVINKVYEYPQGRKIKRQSLFSYFRAKKMDRAIKALAGITIFNM
                     ERKTGVITVSVTTKNRYLSAAVANEYINQLEEYNLGTRKSKAKENEKFIAKRLEEVQG
                     ELTQAENNLEAFQSENRNFYTSTSPELAMELARLEREVEIKSKVFLTLTQQYELARVE
                     AKKDVPIVQVLDYAKPPEQKAGPNRKLIILASVFLSSFVGIGMVLSTELIKTKVKPDE
                     YKMALDLGGEFKGDMLKVYGEVRKPLGWFRRIKIKHKT"
     gene            9821..9934
                     /locus_tag="VGB16_06685"
     CDS             9821..9934
                     /locus_tag="VGB16_06685"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="NAD(P)-binding protein"
                     /protein_id="HEX9917408.1"
                     /translation="MKIGILGGGLTGLVLAYHLKDKAEVLEKEADPNRERS"
     gene            9931..11013
                     /gene="wecB"
                     /locus_tag="VGB16_06690"
     CDS             9931..11013
                     /gene="wecB"
                     /locus_tag="VGB16_06690"
                     /EC_number="5.1.3.14"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_013707134.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008761 - UDP-N-acetylglucosamine
                     2-epimerase activity [Evidence IEA];
                     GO_process: GO:0009103 - lipopolysaccharide biosynthetic
                     process [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="UDP-N-acetylglucosamine 2-epimerase
                     (non-hydrolyzing)"
                     /protein_id="HEX9917409.1"
                     /translation="MKKIILVGGARPNFMKIAPLYFELLKCPQQFAPLIVHTGQHYDQ
                     KMSGAFFKELGLPEPDLNLEAGSGSHAVQTAKIMIAFEEVLLKHKPDLVVVVGDVNST
                     LACALVAVKLKIKVAHVEAGLRSFDKGMAEEINRVVTDSISDYLFTTCEDANQNLIRE
                     GISLDQIFFVGNVMIDSLLGYYSKISQSSVLERYQLKEKEFCLLTLHRSSNVDEKETF
                     YKIMSAIQQISKETKVIFPVHPRTKKMMEKIHIEHQKLIITEPLGYLDFLKLEENAKL
                     VLTDSGGVQEETTFLKVPCLTLRQNTERPVTITSGTNHLVGLDPVAIIKEVDALLENE
                     QRDFALPPLWDGKASQRIVRVLESTL"
BASE COUNT         3375 a         2128 c         2613 g         2947 t
ORIGIN      
        1 atttctcctc cgccaaaggc gagagatctt gagactaatg ccgatgcatc aagaacctgt
       61 ttgatatatg atacacggtg tgggatgtgg ttcaacaagt tcaccatcct gagcaaggtc
      121 gaaggaaatt cccacaccta cgatatgtta tattcgtagc acagggaatt tatccctgtg
      181 cgggttatgt gtttgtcggg tcccccgacc caacagaaaa atactagcaa gtaaaagggt
      241 ttaataggtg caaataatga ggaggaaatt atgattgacg gcgtcaagac taaaaaacta
      301 aaagtaatcc cggatgagcg tggtcgcttg atggaaatct tacgttcgga tgatgagttt
      361 tttagtaagt tcggtcaggt ttatattacc acggtttatc cgggagtagt taaagggtgg
      421 cattatcaca agaaacaaac agacaatatc gcggtgattg tgggtatggc aaagcttgtg
      481 ctttacgaca acagggagga ttctcccacc aaaggtgaaa caaatgagtt tttcatcgga
      541 gagcacaatc cgatgcttat ctgtgttcca aagttagtta tgcacggctt caaagggata
      601 agcaactgcg agactatgat gttgaattgc ccaactgaag tttataatta caagagtccg
      661 gatgagtttc gcctgcctgc tgacaccaag gaaatccctt acaactgggc cagggaggat
      721 aagtgagggc cttggtttgc tctcattcaa attctcaaaa gagaatatac ttaaagaatt
      781 acatctattt tgttatcacc atcacacata aaagatttcc ctattttgaa gaggatattt
      841 tttgtagatt atttatagat gatttgctat tttgccaaga gttgaagaaa tttgagttat
      901 atgcatatac gattatgccc aatcatgtcc atctactcat aaagctaaga ggggaataca
      961 atatttcgag agtgatgcaa tcgataaaaa aggagttctc gagagacata aactacatca
     1021 tcgcaggcga cattccggaa tgtcgccttc aagggaaaca atattcattt cgatataaaa
     1081 aacaatttta tactatctca ttaggaaaat atcgccaggg atttctccaa aaatatgtcc
     1141 agagtccatc gcaattccca cactttagat ggcaaagctc ttttcgtgat cgtattattc
     1201 gtgatgaaaa agatttatat aatcatttga gatacattga gaataattat ttgaaacatg
     1261 gcttggagaa aaacgaattg tcgtggagct gttactcaaa gggaggataa gtgaagcttt
     1321 tggttactgg tggggcggga ttcattggct ccaattttat ccgctatatc ttatctagat
     1381 atcccaatta caaaattatc aatctggata agctcaccta cgcgggaaat ttagataacc
     1441 tctctgatag tgagaagaat aagaattaca cattcataaa gagtgatata acggaccaga
     1501 aattggtaga aaatatccta cgagaaaagc cagatgctat tctcaacttt gcggcggaga
     1561 cgcatgtgga taggtcgatt tacaatcccg aagatttcgt taaaaccaat atagtcggga
     1621 ctggagtttt gatggatgcg gccataaaat acgaaacccc caaattcatt caaatctcca
     1681 cagatgaggt ttacggaagt ttaggaaaga aagggacctt caccgaggaa agcaatttag
     1741 ccccaaattc accttattca gccagcaaag cggcagcgga tttgctttgt cgttcctatt
     1801 ttaagacttt tgatttcccg gtgataatca cccgcagttg caacaatttc ggtccttatc
     1861 aattccccga aaagctcctg ccgcttttca taagcaatgc cctatctgac aacgaacttc
     1921 ctttatacgg cgatggtctt aatatcagag actggattta tgttctggat aactgccggg
     1981 cagttgatat agtcctgcac aaaggaaaaa agggagagat ttacaatatc agctccaata
     2041 atgaaatgac caacttagag atgaccaaac tgatcttgaa aaaactgaaa aaaccagagt
     2101 ccttaatcaa atttgttaaa gacaggccgg gccacgacag aaggtatgct ctggacgcaa
     2161 gcaagctaag aggggatttg ggttggaaac caaaatatag tttcgaagaa ggaataaaca
     2221 aaaccgtgga ttggtatctg ggtaacaaag cttggtggga aaaagtgaaa agcggcgaat
     2281 atttgaacta ttatcgcaag caatatcata atagataaag gtgatgggcg agttttaggg
     2341 gtgtgtcatt tttgcgactc ttcgttttcc ccttgcgttg tcattctgag gcgtagccga
     2401 agaatctcgc ttgcctccca ttccgcccag attcttcact ccgctagcgc tccattcaga
     2461 atgacaaaag cggagtctgc cctgtcttcg actccgcact tcgtcctgag ctcagtgccg
     2521 aaggaaggtc gctcagactc gaggagagtg aaacgaatgg gttcaggatg acagagagac
     2581 taacaattaa atgttactcc cagcaactgg cgggttacag atgaaaaggg ttttagtcac
     2641 cggagcaaat gggcttttag gtcaaaaaat agttgaaatc ctttcccctg cttacaaaat
     2701 cacaagctgc gatttaggtc ctgaattctt cctttcaagt aaagcctcct atcatccctt
     2761 agatattcgt gaaaaggata aagtagtagc cctggtaggg tcctttaagc cgtcttggat
     2821 agtaaactgc gcgtcttata cggatgtgaa tggttgcgaa acaaatcaaa gcttggcttg
     2881 gtcagtgaat gtggatgggg ttaaaaatat agttgaggtt tgcgaaaagc ttaaaataga
     2941 cttaattcac ttctcaacag attacgtgtt tgatggaaaa aacggtcctt atagtgaaga
     3001 agataaaaca gacccaatca acttctacgg aaagacaaaa ttggaaagcg agaaaattat
     3061 tgaggagagt catataaatt tcaccatcat acggacaaat gtgctttatg gatgtgggga
     3121 aaaagtgaaa agtaatttct ttctttgggt tttggagaat ttaaagaaaa atggaaagat
     3181 aaaagtagtg aatgaccaat acaataatcc cactttagcc ttcaatttag ctgaaatcgt
     3241 aaaagaggct gtggatagaa acttgttcgg gctatatcac ataggtggag cagattggct
     3301 atcgagattt gaatttgctc aaaaggttgc cctaagcttt ggatttgata gagataagat
     3361 aaagccaatt cagacctctg atctaaaaca aacagctccc cgacctttga aaggcggcct
     3421 aaagattgat aaagcaaaaa aggttttcaa gacggagatt tgggggatag agaaaagctt
     3481 ggggtttctc aagggaaact gctaaaaagg tttctgcgga atgcaaactt tatggagtgc
     3541 atcctcgggt gcactccata aagtttgtag gggttcgagt agggagctta agatgcaccg
     3601 ctctagaatc tcccctaacc ctcgcctcga ttcggcccgc ctcggcgagc catcggcgag
     3661 acgggcgaag cgggcgtctc ttgcaaagag gggaacaaaa atcccccttt gacaaagggg
     3721 gaaaacaggg ggattttatg gtttgcaggt ttttctggtt tgcggattat tctgatgctt
     3781 caggaatagg aattttccgc ttttagcgga atcctggtga cccgaaccgt atcgtaattt
     3841 cgtgcaggaa ttccaattcc tgcacgaact aaacgcatcg gcatttctcc cgccttcggc
     3901 gaattgggta ggtaatggct gcgccattac catctggggg caagtgcccc tacccgctct
     3961 tgacagatta tttcaaacct atcaatcaca aatattgtag ttgctagttg ggtaggtaat
     4021 ggctgcgcca ttaccattgg ggggtgcaag tgcccctacc cgttatcaga ataaacccta
     4081 ctctttgtga ttttttggga aaaataaaaa ccccccgcct gtggcgaggg ggtttttatt
     4141 tcttttattt gctcaaccca actatctttt tcttctcatt ctaactatag ttccgagtcc
     4201 caaaagtcca gtacccaaaa ggatgaaggt tcccggctct ggagcggcag taaggtactt
     4261 tgagtcaatg ttagtgaaac cgtctgcgtg tgcatagaag ttgtcatgct gaacattaac
     4321 gtaaggagcg ctcccgtagg gtccgtcatt tccagtctcc atgaagactc tcacattgtt
     4381 actgatgcct tcaggaagag tggtactggt tgctaaaacc caggtatctc cagaccagta
     4441 gtaagcaccc aagtaattac ctaaccttgc tattctgaat ctaccagaca tatcgctggt
     4501 aagaccgaaa aagtcaccac tccagtttcc accaatctgg taattcgcgt cgtagtagtt
     4561 attcccattg tcttcataag agcgcgccat cttcattccg aaagaaccat caggagccca
     4621 aacatagagg gaggcgctac tatagaatgc gttgaaatca atcaagtcga aatcaacttg
     4681 ggtatcgaag tcccccgaaa ggttgaactt ggattgtgct cttcctcttc ccccacgaac
     4741 agcttcgttt gaagtaccta aagccaagta gccactttct acagttgccc aaccaccatc
     4801 gtgagagtaa tccatccaca gattggtatt gaagtcgttg aacaagtcag tcataataaa
     4861 gctggcttgc gctgttcctc caaaaagaag cacaactcct aaacctaaga ccaacatcaa
     4921 cttcttcatt caaacctcct taaaattgtt gctttttcag ttttcaattc agttgtcttt
     4981 atatgcaatc tttgtgccaa tagcctacat aaatgttaag tccttaattc acagcagatt
     5041 agcgatagca tatcctttct tgcttcttgt cttgcggctg gaaagtgttt agtccaccta
     5101 cactaagatt ctaacccatg gtcttttatg tgctaagtta ttgattatta atggaatata
     5161 actggaaagg aagtgtaaaa acgcctaaac aaaatctggt gaaaactaga caaggaaaat
     5221 atttcacttg tggcttgaaa tattttcttt tgtagcagaa tgaaatcaaa tgaatatttt
     5281 atataagata ttgtttatca gtgacttata aaacgagact atgtttggca tattttttgc
     5341 tcaaaaatca gacagaaaaa atgacctctc tcgaggattt aagtttaaga aagccgtgtt
     5401 attgtcgata agtgaaaaaa tcaagtctca aaagagttca ggttgccctt tacccctcca
     5461 actttaaagt cgcaaccatt tgtagtttca aggtcattag taggtcttgg agtgtcccgc
     5521 tttatcggga ccaacaataa caatataaag ctgaagcttt atactccaga aaaatttgat
     5581 ttcatagctt accataaaga agcgggtagg gaacggttgc accgttcccg aaaagataaa
     5641 gacagtggaa gtaatggcgc agccattact tacccgattt gatttcatag gaggaaatag
     5701 atgagaaaag tagcttcggg gctaatagtg gcgctagttt tgtctctttt ttgggtgggc
     5761 tttgatttat ctgctcagac acaagaggaa aaagacaagc tctatcaaga gtatcagatg
     5821 aaaaagggta tgccccagga gggtgatatt ccgaaatacc agactcctga gatctatgaa
     5881 gaaaccaagc cggtggtgga actgaaaaag acagccacaa tttcggttcc ctctccggtt
     5941 gcatccgcca gcccagggat tcccaaggca ggtggtgatt tgcagccctt tggatacaat
     6001 atgttcaatg ttactccctc gagcttcgct ccggttacac cgacatctgt tccgccggat
     6061 tataccttag gtccgggaga caacatcata gtcaatcttt gggggaaagt tgatttacag
     6121 ttagacctca cagttgaccg cgaagggaaa atctttatcc ccaaagcagg agacttaacg
     6181 gttgtgggtt taaccttaga ccaatttgag aaaaagctaa aagagaaact ctccaccatc
     6241 tattccggtt ttagaatgag cgtgatgatg ggaaagattc gtttaatcaa agtattcgtt
     6301 tttggagagg tgaccaaccc cggtggttac accataagct ccctttctac tctttttaat
     6361 gccttatatg aagcaggcgg accaaaccag agaggaacgc ttagaaatat aaagttagtc
     6421 cgcaataacc aggagatagc cacaattgac atctacgact ttctcttaaa aggatataac
     6481 aaagatgaca ccaagcttgc ctccaacgat gtgatctatg ttccggttgt gggttcattg
     6541 gtcaaaatca gaggagaact caaaaggcct gcaatatatg agttaaaggg gggagagaaa
     6601 attttggatt taatcgagtt agccggagga tttaaaacca cagcttatct ggaaagggtg
     6661 atggtggata ggattgccgg tcaagacaaa aggatgcttt tagatgtcaa cttgcttgac
     6721 cagtcaaaat caaatgagaa taacttggag cttaaagatg gagatgatgt ttctgtgttt
     6781 tccatctttg acttgataga gaatttcgtg tggatagatg gtaaggtaaa gcattcaggc
     6841 gcttttcaaa gaaatccggg gatgagggtg tgggatttga ttggtggggg ggaacaattg
     6901 cagaaggatg tatatctttt gagggcagac ttaattcgca cctatccgga tgagaggaaa
     6961 gagataatcc cggtgaactt ggagaaagtc ctggccggcg atagcaccga gaatatgatt
     7021 ctaagagata gagacaggtt ggtggtttat ggatattggg atgtaaatcg caaaaaattc
     7081 gtgaacataa atggtgtagt gaaaaaacca ggacaatatg aattatatga gaatataaag
     7141 ctttcggatt tgatcttttt ggcagggaat cctctgaaga acgcatatct attgagagca
     7201 gagatagccc atcttaatcc cggcaaacca gcagacataa gttatgtcaa cttagaagat
     7261 ctatataccg actcggaaaa ttttataata aagccagacc aaaatgttga tatctcctta
     7321 aaagaagatg accagatttt cattcgagag ataccggagt ggagtctcca aaaaatcgta
     7381 accatagaag gggaagtgat gtttccggga aactatgcct tggagcaaga aaaagaggcc
     7441 ttatatcatc tgctccagag agcaggaggg ttgactgata aagcgtttgc caaaggagca
     7501 atctttgttc gaccgagcat tattagggat gtggagagaa aacatgttgg ggatgtggtg
     7561 gtgagttccc agcttctgga aagggatagc ttaggcaaca tacttcctcc agttactacc
     7621 ccctttaatc ccgatagact caatcgggtg attatagatt tgcccaagac attaaggtta
     7681 aacggaggag aagacgatat cgtgcttcac gatagtgact acgtttatat ccccagaatc
     7741 ccttcagagg ttcaggtggt gggaagcgtt ggcgcgagta gcagcatcaa attctacgac
     7801 ggcaagagcg ttaagtatta tatcgacagg gctggcggct acacccgcaa ttcagataag
     7861 ggacaaactc gtctgattgg tgcggacgga agagtcatct caggagggag cttgagagga
     7921 agagaggttg aattgggaga tgtgatcgtg gtcccgcaga agatagcaaa acataaggat
     7981 tggatgaagt taatggctaa tacagttgcg gttctatccg gactagcaac tacaatattt
     8041 gtaattgata ggttgaagta aagcagtttt accctgcacc gtaatggtgc agggtttgag
     8101 ttttgagttt tgagaatttg aatttcttgt gcctgtcagg tcccccctgg ccattgggtt
     8161 gagcggggag aaacgggcag gccgacctga caggggaact ttccgccaag tcaggcgaca
     8221 tggcggatac aaatgagaca tagtctacgt tgacaattta agaactcctt tccctccctt
     8281 gatgggaggg aacaaaaggg agggtgataa tggaatttgg aatcaccctc tccttaatcc
     8341 tcccccatca agggggagga gggtgcggtt ttaaatgtgt aggagtcgga cctgtcccgt
     8401 ttttggcggg atttatccga tccgtaagag ttctggcaaa taaaatgcga gattgccgcg
     8461 ctccccgaaa acaatcgggg tcgctcgcaa tgacaattct tctgttgtca ttgcgaggag
     8521 tcccgccaaa ggcgggacga cgaagcaatc tcatagtcaa actaatgagg tgaatgtatg
     8581 agtgaaattc aaatcaggga aaacaggatc ttagctgaag acttaaaacc aaactccaca
     8641 caggagattc tagttctttt ttcctctttg gtcaaaaaga gaaaatggat ttaccttctg
     8701 gtcggagtca cggtcttggc aacaacgatt ttttgtcttt ttctgcccaa caaatacacc
     8761 gccaaagcag tgattctccc ctcaggcgga acgactgaca agttgggagg actcaaggaa
     8821 ttcactggtt tgatgggcga tttttcttta ggtgtttcgt caagcaatct ggagaatagt
     8881 tcctttcttt acccggatat cctgaaaagc cggctcttaa gcgaagccgt gataaataaa
     8941 gtctacgaat atccccaggg acgaaaaatc aaacgtcaaa gtctattcag ctatttcaga
     9001 gctaagaaga tggacagagc aattaaggcc ttagctggaa taacaatctt caatatggag
     9061 cgaaaaacag gcgtaataac tgtctccgtc accaccaaaa acagatatct ctccgcagct
     9121 gtggcaaatg aatatataaa tcagttggaa gaatacaatt taggtaccag gaaatcaaaa
     9181 gctaaggaga acgagaaatt catcgccaaa aggttagaag aggttcaagg ggaactaaca
     9241 caggcggaga ataatttgga ggcttttcaa tcagaaaata ggaacttcta cacttccacc
     9301 tcccccgaat tggctatgga actggctcgt ttggaaaggg aagtggaaat caagtccaaa
     9361 gtctttctga ccttaaccca gcaatatgaa ttggctcgag tggaagctaa aaaggacgtt
     9421 cccattgtgc aggttctgga ctatgccaag ccaccggaac agaaagcggg accaaatagg
     9481 aaattgataa ttttggcaag cgtctttctt tccagtttcg tgggcatagg gatggttttg
     9541 tcaaccgaat tgattaagac aaaagtgaaa ccagatgagt acaaaatggc gttggattta
     9601 gggggcgagt ttaaggggga tatgctcaaa gtttatggtg aagtcagaaa gccactgggc
     9661 tggtttagaa ggataaagat aaaacataag acctaacgta gggatcgcat ttatgcgacc
     9721 cgctcgagct ggatgaatcg agcccctaca aaatctgtat tctctgtagc ggtcgaattc
     9781 attcgacgct ttggggtaaa aagacaggag taaaccagaa gtgaaaatag ggatattagg
     9841 tggggggtta accggacttg ttctggctta tcatttaaaa gataaggcag aagttctgga
     9901 aaaagaagcg gatccaaata gggagagatc gtgaaaaaga taattttggt ggggggtgcc
     9961 agacctaatt ttatgaaaat cgcacctctt tattttgagc tcttgaaatg tccccaacag
    10021 tttgcacctt tgatcgtgca taccggacaa cactacgacc agaagatgtc tggggcgttc
    10081 tttaaggagt tgggtcttcc ggaaccggac ctaaatttag aggcaggatc tggctcacat
    10141 gccgtgcaga ctgcaaagat catgatagct tttgaagagg ttttgttgaa acataaacct
    10201 gacctggtgg tggtggtagg ggatgtgaac tccaccttgg catgcgcttt agtagcggtg
    10261 aagttgaaaa ttaaagtggc acatgtggag gcgggtttaa gaagctttga taaaggtatg
    10321 gcggaggaga taaatcgtgt ggtcaccgac tccatctcag attatctttt taccacctgt
    10381 gaggatgcca atcaaaattt gataagggag ggaatttctt tagaccaaat cttctttgtg
    10441 ggtaacgtga tgatcgattc ccttctgggc tattattcaa agatctcgca atcgagtgtt
    10501 ttggagagat accagctgaa ggaaaaagag ttttgtcttt tgacccttca tcgctcatct
    10561 aacgtggatg aaaaagagac tttttataaa atcatgagcg ctatacaaca aatcagtaaa
    10621 gagacgaagg tgattttccc ggttcatcct cgaactaaga agatgatgga aaagattcac
    10681 attgaacacc aaaagcttat tatcaccgag ccattgggct atttggattt tctgaaattg
    10741 gaggaaaacg ccaagctcgt attgaccgat tccggaggag ttcaagagga gaccacattc
    10801 ttgaaagttc cttgtttgac tctaagacag aatacggaaa ggccggtgac cataacttcg
    10861 ggcaccaatc atctggtggg gttggatccg gtggcgataa taaaagaggt ggatgctttg
    10921 ctagagaacg aacaaagaga ttttgcgctc cctcctctat gggatggcaa agccagccag
    10981 aggattgtcc gggtacttga gtcaacgctc tgaaaaactt gaggggtttc ttatgatagg
    11041 ccaacttctt gtgttgggct cgg
//
LOCUS       DASWLN010000184         5987 bp    DNA     linear   ENV 20-FEB-2024
DEFINITION  MAG TPA_asm: Candidatus Zixiibacteriota bacterium isolate
            SMAG_U2949 k141_1253058, whole genome shotgun sequence.
ACCESSION   DASWLN010000184 DASWLN010000000
VERSION     DASWLN010000184.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37960594
            Sequence Read Archive: SRR10376091
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Candidatus Zixiibacteriota bacterium (soil metagenome)
  ORGANISM  Candidatus Zixiibacteriota bacterium
            Bacteria.
REFERENCE   1  (bases 1 to 5987)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 5987)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (26-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/12/2024 08:28:26
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 1,440
            CDSs (total)                      :: 1,416
            Genes (coding)                    :: 1,412
            CDSs (with protein)               :: 1,412
            Genes (RNA)                       :: 24
            rRNAs                             :: 1 (16S)
            partial rRNAs                     :: 1 (16S)
            tRNAs                             :: 21
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 4
            CDSs (without protein)            :: 4
            Pseudo Genes (ambiguous residues) :: 0 of 4
            Pseudo Genes (frameshifted)       :: 0 of 4
            Pseudo Genes (incomplete)         :: 3 of 4
            Pseudo Genes (internal stop)      :: 1 of 4
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..5987
                     /organism="Candidatus Zixiibacteriota bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_1253058"
                     /isolate="SMAG_U2949"
                     /isolation_source="soil"
                     /db_xref="taxon:2053527"
                     /environmental_sample
                     /country="USA: Oregon"
                     /lat_lon="42.99 N 123.63 W"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            <1..569
                     /locus_tag="VGB16_06695"
     CDS             <1..569
                     /locus_tag="VGB16_06695"
                     /inference="COORDINATES: protein motif:HMM:NF024878.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=3
                     /transl_table=11
                     /product="HD domain-containing phosphohydrolase"
                     /protein_id="HEX9917410.1"
                     /translation="KKHPVLGVKALFSRRELDEFSMRAAVVAFEHHLNLDLSGYPQVI
                     ATREVNLYTRIVSIVDAYDAMTSGRVYVKTPISPDEALRRMMVKRDVSFDPLLLKVFI
                     NIVGVYPIGSVVILNTGELAVVLGTNSNEINRPKVGIIADQKGKRKEIETVDLTEMDY
                     KANTYKRSILRAIDPRRYKIDISKYVRV"
     gene            complement(566..1114)
                     /locus_tag="VGB16_06700"
     CDS             complement(566..1114)
                     /locus_tag="VGB16_06700"
                     /inference="COORDINATES: protein motif:HMM:NF040144.3"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF5683 domain-containing protein"
                     /protein_id="HEX9917411.1"
                     /translation="MRRLILLLILLILVWFLPSFAQFKQDISQAASRKPVMSGDSLVI
                     LPKKSPFGALVRSVALPGWGQFYNKQSLKGSLIFATETTLLVAMAVEWKRRDEHLRAF
                     NQLPLDSPDKSWEFELYQFHRDNRNLFLWSLAGVVFYSMMDAYVDAHLFNFKQDQVKD
                     ADLTLVPQVGEKEMGIILSVNF"
     gene            2136..2894
                     /locus_tag="VGB16_06705"
     CDS             2136..2894
                     /locus_tag="VGB16_06705"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_012120114.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="NAD-dependent deacylase"
                     /protein_id="HEX9917412.1"
                     /translation="MEDELEVSQALRTELKSTARVVVLTGAGISAESGVPTFRGEDGL
                     WNKFKPEELATVDAFMSNPELVWEWYEFRRNIIERIKPNPAHYTLVEFQKYFEDFTLI
                     TQNVDGLHQRAGSTGVVELHGNINANKCVDCGKRYDDLSQAKPGIPPKCLCGGNIRPD
                     VVWFGEMLPSRATNKAFERSSNCDLFFSIGTSAIVHPAASLPLMAKKNKAYVVEINTS
                     PTGISDFIDELLLGKAGEILPRLGQMIEKKANSR"
     gene            2891..4948
                     /locus_tag="VGB16_06710"
     CDS             2891..4948
                     /locus_tag="VGB16_06710"
                     /inference="COORDINATES: protein motif:HMM:NF024824.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0015291 - secondary active transmembrane
                     transporter activity [Evidence IEA];
                     GO_process: GO:0006810 - transport [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="TonB family protein"
                     /protein_id="HEX9917413.1"
                     /translation="MKQKNLIMKLGLITILLVSLSCAYYNTFYYAKRNFKKAESSQKK
                     ANRQIASPEEKRLYDGAIQKASKVLTFHPKSKWVDDALLVIGMSFYYEGEYSKAERKF
                     SELTSSFPRSELVLKARFYQAMCQYRLEEYDGAISSLKSILADPKSKNLREQIYFTLG
                     EIYFQQKRYDEAIENYQTLIRKYKKSELLAQVQYRIGESYLQKKDYLGAQEALSQVNN
                     FKPANELIFNSAFKTAECYYVMGKISSGIELLNKLSKDERFYQKLSVVRLKIAEGFYL
                     LDKDEEAIKEYQEITTSYPKTEGSAMAYYQLGIIYQERVGDLKKAKEMFDKSKDEKPG
                     SACAKKALEKSASLAKVDEYKKQLSTEEVKETAKTTFLLAELYLTQIDQPDSAIAEYL
                     SLVEHHPQSEYAPKSLYAAAWVLQNIKGDSVAASNLYQRLIEEYPHSDYAKPAYRFFK
                     QPPSSKETYAEEEYLKAEELLLVEGKVDSALALYRKIVGDYPQSEYAPKASYAVAWTI
                     ENLVCPNDSSSILAYQEVVDKFPQTEYATRAKSKLSSTGMPKATPPASLSQNTAPVDR
                     SDTTKSFPDTTQYSIPLAPTPIIRGPFLYPESERDSGIQGKVVLKIKIDYPTGTVAEA
                     DVLGSLNNYNIDEAAKKAAMSTFFSPDSFTVKPPGGYYLYTVDVIRYGTDQYDSHLDR
                     ANF"
     gene            4950..5759
                     /locus_tag="VGB16_06715"
     CDS             4950..5759
                     /locus_tag="VGB16_06715"
                     /inference="COORDINATES: protein motif:HMM:NF012402.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="dihydroorotate dehydrogenase electron transfer
                     subunit"
                     /protein_id="HEX9917414.1"
                     /translation="MIKQISSSILRIEEITEKVFKLILSSSFIAKNGKPGNFIHIKVN
                     ETTTPLLRRAFSLHRADPRKGEIEILFKVVGLGTRILSEKRKGEALDLIGPLGNSFSL
                     PFRNQKAMMVAGGMGLAPLWFLLNELLKNGNIRKGDITFFQGARSKNGILYSKKLSSL
                     GIKHLISTEDGSLGYKGMVTDLLVKEVRIKDKVKIYACGPTGMVSFLSRISRKANLDC
                     EVSLEAHMPCGVGACAGCVMKIENKRIAEYKKVCSDGPIFDAREVYLDEED"
     gene            5746..>5987
                     /locus_tag="VGB16_06720"
     CDS             5746..>5987
                     /locus_tag="VGB16_06720"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_014560478.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="dihydroorotate dehydrogenase"
                     /protein_id="HEX9917415.1"
                     /translation="MKRIDLSVSIGKINFKNPVLVASGTFGYGEEYSKLIDLNKLGGI
                     ITKSITSKPRKGHPPARTCETPAGMLNCIGLENIGV"
BASE COUNT         1821 a         1228 c         1394 g         1544 t
ORIGIN      
        1 taaaaaagca tccagttctg ggagtcaaag ctctcttctc cagacgagag ctagatgagt
       61 tctccatgcg cgcagcagtg gtggcttttg agcatcatct caatctggat ttgtccggtt
      121 acccgcaagt gattgccacc cgagaagtga atctttacac ccgcatagtc agcatcgtgg
      181 atgcttatga tgctatgacc tctggccggg tctatgtcaa aacacctatt tccccggatg
      241 aggctttacg aaggatgatg gtgaaaagag atgtttcctt cgacccccta ttacttaaag
      301 tcttcatcaa catagtggga gtttatccta taggttcggt ggtgatttta aataccgggg
      361 agttggcagt agttttggga accaattcca atgagatcaa tcggcctaaa gtgggaatta
      421 tcgctgacca aaaagggaaa aggaaagaga tcgaaacagt cgatttgacc gaaatggatt
      481 ataaggccaa cacttacaag agatcgatcc taagggcaat tgacccgcga aggtacaaga
      541 ttgacatatc aaaatatgtg agggtttaaa agttaaccga taggataatc cccatctctt
      601 tttctcccac ttgtggcacc aaagtcaaat ctgcatcttt cacctggtct tgcttgaagt
      661 tgaataggtg ggcatctaca taagcatcca tcatgctata gaacacgacc ccggctaaag
      721 accaaagaaa caggtttctg ttgtctcggt gaaactggta cagctcaaac tcccaagatt
      781 tgtccggaga gtcgagcgga agctggttaa aagctctcag atgttcatct cttctcttcc
      841 attcaactgc catcgcaaca agcagtgtag tttcagtggc gaatatcaag cttcccttta
      901 aagactgttt attgtaaaac tgcccccagc caggcagggc aactgaacgc accagagctc
      961 caaaagggga tttcttgggt aagattacga gagaatcgcc agacatgacc ggcttcctgg
     1021 aggcagcttg actaatatct tgtttgaatt gggcaaaaga aggtaaaaac cagaccagaa
     1081 ttagcaaaat tagaagcaaa attaaccttc tcattctact cctttgcaac tcaaaccaag
     1141 atataacctc tccacataaa agacaaggat tttttaactg cacctttccc ctttctcatt
     1201 ttagaaggga tggatagaat tttccccgga ggtatgaacc attacggttc ataccaaaga
     1261 gatgaataac atccttatgc gaattcctcg aattctcagg tcttgagata cacgttgaaa
     1321 tcaattcttc ctctcccctg gatgttgggt gagaggggag gcagatttgc aggcgacgca
     1381 tctgggtcgc ctaccagctt agatagagag aaactctctc ccctatgggg agagagatag
     1441 agtgagggga gaatgaactt taactcgtcc cctcacctct gccctctccc tttaaaggag
     1501 aaaagataat ctccaagcaa accagagtgg cttgaggatc aaaagccaat cctcacagtg
     1561 gattttgagt ttacttattc tttctactga ttcatatttc tgtgcccgtc aggtcccctc
     1621 acctgacgga atatttctgt cgggttgggg aacccgacag acacataagg tggggttacg
     1681 gatttatttc gttggttcgg gaattgtcac caggatcccg cttgggggag aattgcgaaa
     1741 gaacctttgt ttgctattgc gttggtcaga cattcttgtc tgacttgtct gataacaaag
     1801 gaataccagg atgtcatcca cattttgctg ttccgtcagg agctactcct gacggtcaaa
     1861 gaatctgagg aacaatgtca ggactaggtc ctgacataac tggaaaaaga acagacaaga
     1921 atgtctgttc taccaaattc ttttcaagtt ggctttttgg catagagcat cgcagtccgt
     1981 ggtttgtatg tagaagagtt ttgagagcta aagcgaaagg caatcgattc acaatcacca
     2041 aaaagattcc cttgctccct taccgatacc tataactttt tttaatttac agaaaaatct
     2101 tgattagcaa gtgataagtt tgtataataa cccagatgga agacgaattg gaggtaagtc
     2161 aagctttaag aaccgaactg aaatccactg ctcgcgtggt agttctaact ggggctggaa
     2221 tctcagctga aagtggagtt ccgacctttc gaggggagga tgggttgtgg aataagttca
     2281 aacccgaaga gttagccacg gttgatgctt ttatgtccaa tcccgaattg gtctgggaat
     2341 ggtacgagtt tcgcagaaac ataattgaaa gaataaaacc caatccagct cattatacct
     2401 tggttgagtt tcagaaatat tttgaggatt ttactctgat tacccaaaac gttgacggat
     2461 tacatcagag ggctggctca actggtgtcg tagagctgca tggcaatatt aatgccaata
     2521 agtgtgttga ttgtggcaaa aggtatgacg atttaagcca agccaaaccg ggcatacctc
     2581 caaagtgcct gtgcggggga aacattcgtc cggatgtggt ctggttcggg gagatgctgc
     2641 ccagccgtgc taccaacaaa gcctttgagc gttcctcgaa ctgcgacctt ttcttctcca
     2701 tcgggacttc agccatcgtt catccagcag cctctctccc tttgatggcg aagaaaaata
     2761 aggcatatgt ggtggagata aacaccagcc caaccgggat atccgacttc atagatgaac
     2821 ttcttctggg caaagcaggt gagatattgc caagactagg gcaaatgatt gaaaagaaag
     2881 caaattcaag atgaaacaaa agaatcttat aatgaaattg ggtttaataa ctattcttct
     2941 ggtctccctt tcctgcgctt attacaacac tttctattat gccaagagga atttcaagaa
     3001 ggcggaaagt tcacagaaga aagcgaatcg tcagatagct tcaccggagg agaagaggct
     3061 ttacgatgga gccattcaga aagcctccaa ggtcttaacc tttcatccca agagcaagtg
     3121 ggtagatgat gcccttttgg ttatcgggat gtcattttat tatgaagggg aatatagtaa
     3181 ggcggagaga aagttctcag agttaacttc gagtttcccc agaagcgaac ttgtcctgaa
     3241 agcacggttt taccaagcta tgtgccaata ccgcttagaa gaatatgatg gagccataag
     3301 ctccttgaag tcgatactgg ctgaccccaa aagcaaaaac ctaagagagc agatttattt
     3361 tactctgggg gagatatatt ttcagcagaa aagatatgat gaagccatag agaactacca
     3421 gaccttgatc cgtaagtata aaaagagcga attgttagca caagtccagt atagaatcgg
     3481 ggaaagctat ctgcagaaga aagattatct cggggctcaa gaagccctca gccaggtaaa
     3541 caatttcaag ccggcaaatg aacttatttt caattccgcc tttaagactg cagaatgcta
     3601 ctatgtgatg ggtaagatca gttcgggcat cgagctttta aataaactga gtaaggatga
     3661 gaggttttac caaaaacttt ccgtagttag gctcaaaata gcggagggat tttatctttt
     3721 ggataaagat gaagaagcca taaaggagta ccaagagata accacctcct atcccaagac
     3781 cgaagggtcc gctatggcgt attatcaatt ggggataatc tatcaggaga gagtaggcga
     3841 cctgaagaaa gctaaggaga tgtttgataa aagcaaagac gagaagcccg gctcggcttg
     3901 tgccaaaaag gcgctggaga aaagcgccag cttagccaag gtggatgaat acaaaaaaca
     3961 gctttcaact gaggaagtta aggaaacagc caagacgacc tttctcttgg cggagctata
     4021 tctcacccaa atagatcagc cggattcagc catagctgaa tacctctcct tggtagagca
     4081 tcatccgcaa agtgagtacg cgccgaagtc tctctatgct gcggcttggg ttctgcagaa
     4141 cataaaagga gactcagtcg ctgccagtaa tctttaccaa agattgattg aagagtatcc
     4201 ccattccgac tacgccaaac cggcctacag gttttttaaa cagccccctt cctcaaagga
     4261 aacctatgct gaagaagagt atctgaaagc ggaagagctt ttgctggtgg aggggaaggt
     4321 tgattcggct ttggctttgt atcgtaagat agtgggggat tatcctcaaa gcgaatacgc
     4381 tcccaaagcc agctatgccg tcgcttggac catagaaaac ctggtctgtc caaacgactc
     4441 ctcaagtata ttagcctatc aagaagtggt ggacaagttc ccgcagacag aatatgcaac
     4501 tcgtgccaag tcgaaattga gttcaaccgg gatgccgaaa gcaactccac ctgcttctct
     4561 ttctcagaat actgctccag tagacagaag cgataccact aaatcctttc cagatactac
     4621 tcaatatagc ataccactgg ctcccacccc tatcattcgc gggccttttc tttatcctga
     4681 atcagaaagg gatagcggga tacagggaaa ggttgttttg aagataaaga tcgattaccc
     4741 tactggcact gtagctgaag cggatgtgct cggctcatta aacaattaca atatagatga
     4801 ggcagccaaa aaagcggcta tgagcacctt ttttagtcca gatagtttca cagtcaagcc
     4861 gccaggggga tattacctct atacggtgga tgtgataaga tatggcacgg accagtatga
     4921 ttcacattta gaccgagcca acttctaata tgataaaaca gatttcaagt tctatcctta
     4981 ggatagagga aatcacagag aaagtattta agctcatctt atcttcttct tttattgcta
     5041 agaacggaaa gccgggaaat ttcatccaca taaaagtaaa tgaaacgacc acacctcttt
     5101 taagaagagc ttttagcttg catagggccg atccccgaaa aggggaaatc gagattctgt
     5161 ttaaggtagt gggtttggga acaaggattc tatctgaaaa gagaaaaggc gaagccttag
     5221 acttaatagg tcctctggga aactcctttt cccttccatt tagaaatcaa aaagcaatga
     5281 tggtggctgg cggtatgggc ttggcaccac tctggttttt actaaacgaa cttttgaaaa
     5341 acggaaatat aagaaaaggt gatatcactt ttttccaagg agccaggagc aagaatggaa
     5401 tcttatattc aaagaaactt agctctttgg gcattaagca tctgatttca actgaagatg
     5461 gaagcttggg atataagggg atggtaactg atttattggt taaagaggtg aggataaaag
     5521 acaaggtgaa aatttacgcc tgtggaccaa caggtatggt aagctttttg tctcgaattt
     5581 caagaaaagc gaatttagat tgtgaagttt cacttgaagc gcatatgcct tgtggagttg
     5641 gtgcttgcgc tggatgcgtg atgaaaatag aaaataaaag gattgctgaa tacaaaaaag
     5701 tctgttcaga tggtccgatt tttgatgcac gggaggttta tttagatgaa gaggattgat
     5761 ctgtcagtta gtatcgggaa aataaatttt aagaatccgg ttctagtagc atccggaact
     5821 ttcgggtatg gagaggagta ctccaaacta atagatttaa acaaactggg cggcatcatc
     5881 accaaatcaa taacttcaaa accgagaaag ggacacccac ccgcaagaac ctgcgagact
     5941 ccagctggaa tgttaaactg cattggtctg gagaatattg gggtgga
//
LOCUS       DASWLN010000185        33587 bp    DNA     linear   ENV 20-FEB-2024
DEFINITION  MAG TPA_asm: Candidatus Zixiibacteriota bacterium isolate
            SMAG_U2949 k141_1136034, whole genome shotgun sequence.
ACCESSION   DASWLN010000185 DASWLN010000000
VERSION     DASWLN010000185.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37960594
            Sequence Read Archive: SRR10376091
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Candidatus Zixiibacteriota bacterium (soil metagenome)
  ORGANISM  Candidatus Zixiibacteriota bacterium
            Bacteria.
REFERENCE   1  (bases 1 to 33587)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 33587)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (26-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/12/2024 08:28:26
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 1,440
            CDSs (total)                      :: 1,416
            Genes (coding)                    :: 1,412
            CDSs (with protein)               :: 1,412
            Genes (RNA)                       :: 24
            rRNAs                             :: 1 (16S)
            partial rRNAs                     :: 1 (16S)
            tRNAs                             :: 21
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 4
            CDSs (without protein)            :: 4
            Pseudo Genes (ambiguous residues) :: 0 of 4
            Pseudo Genes (frameshifted)       :: 0 of 4
            Pseudo Genes (incomplete)         :: 3 of 4
            Pseudo Genes (internal stop)      :: 1 of 4
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..33587
                     /organism="Candidatus Zixiibacteriota bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_1136034"
                     /isolate="SMAG_U2949"
                     /isolation_source="soil"
                     /db_xref="taxon:2053527"
                     /environmental_sample
                     /country="USA: Oregon"
                     /lat_lon="42.99 N 123.63 W"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            313..1830
                     /locus_tag="VGB16_06725"
     CDS             313..1830
                     /locus_tag="VGB16_06725"
                     /EC_number="3.4.21.107"
                     /inference="COORDINATES: protein motif:HMM:TIGR02037.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0030288 - outer membrane-bounded
                     periplasmic space [Evidence IEA];
                     GO_function: GO:0004252 - serine-type endopeptidase
                     activity [Evidence IEA];
                     GO_function: GO:0005515 - protein binding [Evidence IEA];
                     GO_process: GO:0006508 - proteolysis [Evidence IEA];
                     GO_process: GO:0006950 - response to stress [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="Do family serine endopeptidase"
                     /protein_id="HEX9917416.1"
                     /translation="MQKLGFKTWSSLLVASLIGVVLGSVAVMVLFAKVFPLNVSLDQK
                     PSFYEGAKRINVLSTTEENITSTRENAIVRAAKRVGPAVVSISVVQTRIIRATPYSSP
                     FGDPFFDEFWGQFFGSREYKQKIYGLGSGVIINPDGYILTNQHVVQDADEVKITLTNS
                     EEYSGEVVGQDVTSDLALVKIKAKSLPYAALGNSDDLMIGEWAIALGNPFGYLLNDAN
                     PTVTVGVISALGRDIKRERGQLQVYRNMIQTDAAINPGNSGGPLVNADGEVIGINTFI
                     FTTSRGSEGVGFAIPISRAKGIIADLIKYGEVKKAWIGIESQRTSSSSGQDVNREKNR
                     GVVIAQVDKDSPAEKAGLKKGDIITQIGTQNINNQSDWDEIVSLAKAGETLNVAYLRE
                     GKEMKTDIICEPLPAVSVQRFDDNSGMTLASITPSLASQLGTDNQKGVVIVEVKHGSQ
                     ADYLGLEPGDIIRQMENYTIDDLSKYKSIMKDLTNRKVALLVEREGEVYYVSVTR"
     gene            complement(1911..6917)
                     /locus_tag="VGB16_06730"
     CDS             complement(1911..6917)
                     /locus_tag="VGB16_06730"
                     /inference="COORDINATES: protein motif:HMM:NF012311.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="FG-GAP-like repeat-containing protein"
                     /protein_id="HEX9917417.1"
                     /translation="MKKQSLFFLLIFLFILLYLPQSSYSIYSGKTKQAQPQFSPTRLI
                     VKLKPEVDKKVSLGKVSGNVVTGVAFLDSLNVRFDVRKQERLFKEFKKTALKVGRFSS
                     IYTLEVPEGTDLRKMKEEYERRAEVEYVELDYKVELFEQPNDPLFAHQWYLDNTGAAQ
                     NEGQGYYGINRPAGHTLVMKFGTTDADIDALEAFQRADEKVIPLVGIIDTGVDLDHED
                     LKDNIWVNPGEDLNGNGIIEPSEVNGTDDDHNGFVDDFYGWDFSGNDDEMIEDNDPTD
                     YFGHGTHCAGIVAAVRNNGIGVSGINTPCRIMAIKFFPNAFMSLGAKSIIYAADMGCD
                     VINMSWGAPYPSSVVEDAIDYAVSKGVLPIAAAGNSGAEDNLYPASLPQVLAVGASNS
                     KDEVTYFSTYGNQIEVVAPGEDILSLRADNTDMYEENGYPLTHIIDEKYYLADGTSMA
                     SPCAVGVAAYILSASPGISNDSVISIMEQSADDIIYPYGGDSLSSPGKDIYSGYGRVN
                     LNSALALISGRLSKIDYPYENTVVSGEVAIIGTASGANFVSYILEYGEGASPQSWTQI
                     TSSTTPVAKDTLGIFNASGLTGLYSIRLTVGDQNQVVVHVIVNSSVYVKISSPQEGDT
                     IQGYTQISGYTIIPGFSYYTLEYGQGESPSSWTPIDSSTKIVADYILGNWLTSFLNEG
                     NYTLRLTAHSNTDQTYADSISIFVKSITSGGWIQDLSSYGSLSPAIGDIDGDGYDEIV
                     VGVGSMGGIGTPGGVEVFSHDGQREAGWPKDINYYMSSSPALGDLDNDGIDDIVICSN
                     LGVHAYLSSSPSWFRSANTGGKVAMLSFATPVLADLENDGYLEVLTINDTLGIVYAWH
                     KDGQPVIPGNDGVFAQAVMSSGEGSPCLTVTDLDRDGKNEVIVGAADWNDYPMYFGGI
                     YIWDIEGNLILGPGDYPDIFGAVFGIAIANVDENEDLEIVTFGENGEYAALSAFKKDG
                     TQVSGYPIIIEDLISGNWLGNPPAIGDLDGDGILEIVVSVWTLGEARIYAWHQDGTPL
                     GIVGPGGSLVSMKTPGGEKIRQALSSLGNNLREVLTKFKNMSKEELNSLASSLYQDPV
                     FASVAESFGSPVLADVNEDGNVDILARAGYVFGSGYERVFAWDYDGREIDGWPLYASA
                     QERSFTFCSYSPIMADVDKDGKLDMVLGTEDPKLTCWKFINCYDSTTMPWPKYLHDKW
                     NSGRYGFKPTEGEIVNLPPYNFHVKSSNDSSVTLAWNPKPSWASLGYNIYRATTSGEP
                     GDTINTNLIPQPDSQYQDLGLDSGQTYYYTITNVDTNFQESDHSREISAITVQPFVPL
                     FDARIDYAVGSSPRSLFCADLDGDSDLDLAVANAISGNVSILKNNGEGTLQTKVDYDV
                     TFYAQSVFCADLDGDGDLDLAVANSGSNSISILRNDGDGTFQTKVDYAAGSGPRSVFC
                     ADVDGDSDLDLAVANYTTGNVSILKNNGDGTFETKVDYGVGIKPISVFSADLDGDLDF
                     DLAVANSGSNSISILRNNGDGTFEGKVNYGVGVDPYSVFCADLDGDLDLDLAVANGAS
                     NNVSILFNQTNASYVGEEIGDAKPSSFILSQNYPNPFNSETVIEYSLPKASQVKIAIY
                     NILGQKVTTLLDKVEPAGFRRVIWDGKNQNGEIVSSGIYFYRIEAEGFVQSKKMLLLK
                     "
     gene            7318..8895
                     /locus_tag="VGB16_06735"
     CDS             7318..8895
                     /locus_tag="VGB16_06735"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEX9917418.1"
                     /translation="MNRLFKTTALSIILILGLGCSFAKYDLNRIRQQDEAFTKEIMLS
                     SYETDAYGKESHIAEIYGKHEKLFTDENLVRFLKDRIEKERNPRERKKAEFLYRDISS
                     SFLGERTKELDDRIFDLQAKEKIVVEKDTIAFRDIDVLLSNEKNGERRKALYYSQGEM
                     IIDKINPLLTQELNILEKTVKELGYRNYSDYQQQVRGIDFDSLEENCREILKETAPIY
                     KALLSEMARKILNKRMEDVKVYDRAWLFRGESFDEYFPKEKMVPIFKSTMLDMGIDVG
                     AQKNISLDDEDRPEKEPRPATYTIVVPTDVRVLVKPMGGYSDYGSLFHEMGHAEHSAY
                     TRVSEFEFKDLGDYGVTEMFAYILEGIVSNPIFMKDKLGMSQKDIKEFTKMNLFYSLS
                     SLRSYCGKFIFERQLHSGNPDPIKVYQETSKEALLLDRSHKEAEMIYLLYGDERFYSV
                     NYLEAWILDAQLRAKLKERFGENWFEKKEAGEYLESLWTTGMEYTSPEMAKLIKYDKM
                     DPSYLVKDIKQLYDSVK"
     gene            8914..9105
                     /gene="rpmB"
                     /locus_tag="VGB16_06740"
     CDS             8914..9105
                     /gene="rpmB"
                     /locus_tag="VGB16_06740"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_017824130.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0000311 - plastid large ribosomal subunit
                     [Evidence IEA];
                     GO_component: GO:0022625 - cytosolic large ribosomal
                     subunit [Evidence IEA];
                     GO_function: GO:0003735 - structural constituent of
                     ribosome [Evidence IEA];
                     GO_process: GO:0006412 - translation [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="50S ribosomal protein L28"
                     /protein_id="HEX9917419.1"
                     /translation="MAKVCDICGKKPLYGNKVSHANNRTARRWMPNLQRVRALVNGKE
                     KVISVCTSCIKAGKVQKVK"
     gene            9185..10426
                     /locus_tag="VGB16_06745"
     CDS             9185..10426
                     /locus_tag="VGB16_06745"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_014559731.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="multiheme c-type cytochrome"
                     /protein_id="HEX9917420.1"
                     /translation="MLLRTTLIFLTSLFLASGLNAQVCVNCHKSITPKIVSDWQISKH
                     SQNGVDCSVCHGNKHTSPKNVAQAEIPTPETCAACHQTQVDEFKAGKHSLGWASMKAM
                     PTAHWQPMALMEGMKGCGGCHKIGLKTEAEINELKKSGAGFGLASCDACHTRHIFSAV
                     EARQPQACQTCHMGFDHPQWEMYSSSKHGVRYLLKQSKTLPETVSAPTCQTCHMQKNN
                     HAVRTAWGFLAVRLPLPEDKKWAADRMMILQGLDVLGLDGYPTPRLEVVKAADIVRTT
                     QEDWQKERDKMIKTCSQCHSVNFAKEELAKGDEMIKQSDGLMAEAIHVVADLYKDSII
                     TKPQNYAYQFPDLLTFHDAPTVIEQKLFVMFSEHRMRTFQGVFHSNPDYTFWYGWSEM
                     QRDLTEIKTMAEELRRNYKNK"
     gene            10457..11224
                     /locus_tag="VGB16_06750"
     CDS             10457..11224
                     /locus_tag="VGB16_06750"
                     /EC_number="1.1.1.47"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_019599421.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016491 - oxidoreductase activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="glucose 1-dehydrogenase"
                     /protein_id="HEX9917421.1"
                     /translation="MNRVKDRVAIVTGGALGIGQAACLLLAKEGARVAVTDILDKEGE
                     EVVRNIKSKGGVAGFWHMDVSNEKEVQKVFADVDKQFGKLDILVNNAGISGPSKPTDE
                     LTEEEWDRVMAINVKGVFFCTKHVIPYMRKAKGGSIINLSSIYGIISAPDVPPYHASK
                     GAVRIMTKTDAFLYAKDKIRVNSVHPGFIWTPMVENYAKSQGNVEEVRKYLDSLHPIG
                     HVGEPDDIAYGILYLASDESKFITGSELVIDGGYTAR"
     gene            11259..11669
                     /locus_tag="VGB16_06755"
     CDS             11259..11669
                     /locus_tag="VGB16_06755"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEX9917422.1"
                     /translation="MRAEHRNIQKWIKARYGNKYNVEAEIDGQNYLPDRVRHWYQPDV
                     VLRSSKDEIKCIIEVENNPMRKALVGASVLADCSVAALKQQNKPRLIFVVYTKIGIKQ
                     IPNFKQKLKITKKYYLHLKSIRIYSELDFKALQL"
     gene            12007..12465
                     /locus_tag="VGB16_06760"
     CDS             12007..12465
                     /locus_tag="VGB16_06760"
                     /inference="COORDINATES: protein motif:HMM:NF027567.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="pyridoxamine 5'-phosphate oxidase family
                     protein"
                     /protein_id="HEX9917423.1"
                     /translation="MSSSDKKKIVAIMKGNSSHAYLATCDGDQPKVRSMSTIVEDDMN
                     IWMATSSNSRKVKQIKKNPKVCLAFVKQPNGDKSAFVMGKVKIINSLKDKKRVWKLSG
                     YDMCQYFKDGPQSKEFCLLKVIPKKIEWWDSWKSGRKIYKPKKKQVKSIN"
     gene            12490..13203
                     /locus_tag="VGB16_06765"
     CDS             12490..13203
                     /locus_tag="VGB16_06765"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEX9917424.1"
                     /translation="MQRLLLKAFCFLFIFFLIVLYNVTPVIAQVKSDCSFPKLLLVGY
                     NKEGSKLGDLYQQKQTRFKLKSENVGLCYSLLGTFVPIGTGILIWILDEPYYVEHDRP
                     DRYGGDWLEKKDPSRVIPVALIASGLIVGPSMGYFYAGESWRGFKGVAIRLGTGGITV
                     LCMALCYDSDLDNYATLFGGIGFISSIVFLIDVIYDIAKVKSTVRKHNLALHKTALIL
                     APRYFVDSKACGVGLQIKF"
     gene            complement(13465..15090)
                     /locus_tag="VGB16_06770"
     CDS             complement(13465..15090)
                     /locus_tag="VGB16_06770"
                     /inference="COORDINATES: protein motif:HMM:NF015983.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003824 - catalytic activity [Evidence
                     IEA];
                     GO_function: GO:0051536 - iron-sulfur cluster binding
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="radical SAM protein"
                     /protein_id="HEX9917425.1"
                     /translation="MQESLRRKIISTEKGAILKRNAPTKVALIFPNDYSVGMANLGFQ
                     TIYKLLNQIPNISCQRFFLDLGIESLEEKRKLNTFSLISFSLSFEMDYPNVLKILSQA
                     GIPLTHSKREENHPLVVAGGIAPSLNPEVMAPFFDCILVGEAEEMIMEFMKTYLEYSF
                     KKSKKDELLAKLARIQGVYVPRFYEVECDSKGYLKRIEKKNDVPLPIKPRKVDLDKYY
                     TFSPVTSPYIHFKDTFLIEVGRGCKRGCRFCAAGFAYQPTRYVPKENILRQIENYIGK
                     NRQVGLVGTLVSDYPDFEKVCQELKQRGLGIKTSSMRIDRLNSNLLKYLSGSGMKTLT
                     LAPEVGSSRMQKIINKKIDLDEILKSIELAKQSNINSLKLYFMLGLPFEKDEDIDAMV
                     ELLKNIHQIYFKDSSAKRTITLSINPFVPKAHTPFQWAGMNKKEELEQKYIQIKGGIS
                     KLRGIKFQGKGIKGFYLQALLSLGNRIVGEGLYHEQTEGMNIYSFLKQRRFDIEHLLF
                     EEKKEDGVFPWEIVEHQIPKQFLLREYKRAKSI"
     gene            complement(15195..16349)
                     /gene="ftsZ"
                     /locus_tag="VGB16_06775"
     CDS             complement(15195..16349)
                     /gene="ftsZ"
                     /locus_tag="VGB16_06775"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_014521294.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003924 - GTPase activity [Evidence IEA];
                     GO_process: GO:0000910 - cytokinesis [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="cell division protein FtsZ"
                     /protein_id="HEX9917426.1"
                     /translation="MAFELDAEIENYAKLKVVGVGGAGGNALNHMVDASLAGVEFISV
                     NTDAQALETSKVSNRVQIGSRITRGLGAGANPEIGRRAIEEDRELVENALKGADMVFV
                     TAGMGGGTGTGAAPIIAEIAKEQGALTVAVVTKPFTFEGKKRMARAINGIDQLKERVD
                     TLISIPNQRLLAIVNKETKLTDAFKMADDVLLQATRGISDLITVPGLINLDFADVKTV
                     MSEMGDALMGTGYGKGENRASEAAHSAISSPLLDEISIVGARGVVVNITGGKNMTLFE
                     VNEAASIIFENAGADANIIFGAVIDSNLKDEMKITVIATGFGMDMPLRKGLKQDEDII
                     DLFGHRKSLTERVNSLNKDAKSQVVIQKNRVKSFATDDLEVPAFLRKQTE"
     gene            complement(16371..17606)
                     /gene="ftsA"
                     /locus_tag="VGB16_06780"
     CDS             complement(16371..17606)
                     /gene="ftsA"
                     /locus_tag="VGB16_06780"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_004512388.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005515 - protein binding [Evidence IEA];
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_process: GO:0043093 - FtsZ-dependent cytokinesis
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="cell division protein FtsA"
                     /protein_id="HEX9917427.1"
                     /translation="MGNNNLVVGLDIGTTKIAAIIAELGEQGEPKIIGVGVSPSEGLR
                     RGVVIDLEKTVQSIVKAVEDAELMAGIEVNAVYAGIAGDHIRSINSRGVIATSRSDNE
                     IGPADVERVINAAKAVAIPMDREIIHVLPQEFTVDDQSGIKDPVGMSGVRLEAEVHIV
                     TGAVTSAQNIYKAIKRAGLEVLDLVLEPLASSYALLNPDELELGVVLIDLGGGTTDMA
                     VFYDGSIRHTAVIGLGGKNVTNDIAIGLRTPVDKAEELKVTYGCALSSLVDPEEIIKV
                     PGVGGREPREVSRNVLSSVIEPRMEEIFSLAQREKKRTTLADMLAAGVVLTGGGSLME
                     GAIELAEQIFDMPVKQGTPRQISGLVDIASSPIHATGVGLVIYGFQRPRKGKYRSTAA
                     KNIFGKAFHKTKNWFKEYF"
     gene            complement(17609..18319)
                     /locus_tag="VGB16_06785"
     CDS             complement(17609..18319)
                     /locus_tag="VGB16_06785"
                     /inference="COORDINATES: protein motif:HMM:NF015739.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="FtsQ-type POTRA domain-containing protein"
                     /protein_id="HEX9917428.1"
                     /translation="MLIIRNLLVAFLLTLALIGTFIFLTNFPGFKIKAIQIEGNQRVS
                     YSDILSLCPVKLGQNFFEIDMDGIRKKILSEPRIETVKIERRFPQKILINLSEKKPIY
                     LINLGDMYGLTQNGEIIPLEENLDLPVVNGINLKKATFYHKIRDRKIDFVLFLHNSLA
                     KIDANILNLVSEINLEEKDNVILYLIPRGAKIFLGCGEFEKKLIRLDLILKEEKELEK
                     IEYVDLRFKGQAIVRRFE"
     gene            complement(18323..19696)
                     /gene="murC"
                     /locus_tag="VGB16_06790"
     CDS             complement(18323..19696)
                     /gene="murC"
                     /locus_tag="VGB16_06790"
                     /EC_number="6.3.2.8"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_014854993.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008763 - UDP-N-acetylmuramate-L-alanine
                     ligase activity [Evidence IEA];
                     GO_process: GO:0009252 - peptidoglycan biosynthetic
                     process [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="UDP-N-acetylmuramate--L-alanine ligase"
                     /protein_id="HEX9917429.1"
                     /translation="MKFKKVKQIHFVGIGGAGMCGLAEVLFNSGYQVTGSDISLGEVT
                     EKLKSLGIKVFGGHSKENIEGADVVVISSAVKPSNVEVTYALEKSIPVIRRAEMLGEL
                     MRMKYGIAIAGTHGKTTTTSMVGQILTEGGLDPTIIVGGKVVNLDSSAKLGQGEYLVA
                     EADEFDRSFLRLSPMIAVITTLEAEHLDCYQTLDQLKSAFVEFANKVPFYGRLILCLD
                     EESLQNLIPKLKGPITTYGLMPQADMQAENIKLSENRSRFTVKYKGEELGNIELAVPG
                     MHNAKNSLAAASVGLELDIPWEKITLALRQFRGVKRRFEIKGESKGIMIVDDYAHHPT
                     EIEATLKAAKNGWNRRIVVVFQPHLYSRTKDFAEQFGKAFFNCDILVVTDIYAAREEP
                     MPGVSGELVSQAAKRYGHKQVHYKPKSEEILPFLLDTLKPGDMLITMGAGDIYQKAEQ
                     LLNLLQK"
     gene            complement(19693..20796)
                     /gene="murG"
                     /locus_tag="VGB16_06795"
     CDS             complement(19693..20796)
                     /gene="murG"
                     /locus_tag="VGB16_06795"
                     /EC_number="2.4.1.227"
                     /inference="COORDINATES: protein motif:HMM:TIGR01133.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0050511 -
                     undecaprenyldiphospho-muramoylpentapeptide
                     beta-N-acetylglucosaminyltransferase activity [Evidence
                     IEA];
                     GO_process: GO:0009252 - peptidoglycan biosynthetic
                     process [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="undecaprenyldiphospho-muramoylpentapeptide
                     beta-N-acetylglucosaminyltransferase"
                     /protein_id="HEX9917430.1"
                     /translation="MVRIIIVAGGTGGHLFPGIALAQELRKRYKDIELFFVGTKRGVE
                     SRELSKLGFNLETISIVGLSRKNILRNISAPFYLFKGLAQSFMLLNRIKPGVVIGTGG
                     YVSYPVLLLASLKKILTLIQEQNSFPGMTTRLLAPRVDKVCLTYPGSLKYFPSFTSKP
                     KKFKILGNPIRKEVTQGNRDLGLKRFNLSPDKKTIFIFGGSQGSHKINLTVLEALDYF
                     GDNLQLLWQTGEKDFDATKEKTREIKMNLSLHPFIDDMGSAYAVADLVISRAGALTLA
                     EITACGKPAILIPYPSATADHQKLNAQELEKSGAAKLILENDLNGKKLANLSNRLFAD
                     KSVLEQMSQASRNMAKLDATSKITDEIEELLKR"
     gene            complement(20790..21917)
                     /gene="ftsW"
                     /locus_tag="VGB16_06800"
     CDS             complement(20790..21917)
                     /gene="ftsW"
                     /locus_tag="VGB16_06800"
                     /inference="COORDINATES: protein motif:HMM:TIGR02614.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0016020 - membrane [Evidence IEA];
                     GO_function: GO:0003674 - molecular_function [Evidence
                     IEA];
                     GO_process: GO:0009252 - peptidoglycan biosynthetic
                     process [Evidence IEA];
                     GO_process: GO:0051301 - cell division [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="putative lipid II flippase FtsW"
                     /protein_id="HEX9917431.1"
                     /translation="MTDKSRPDYQLLLVVAILVAIGIVMVYSASAMVGDERHKSTIYF
                     FKRQLLWFIMAFPVMLLAMRLDYHIFQKISLPLLLLSLFFLVAVLLRPPMNNVRRWIR
                     FGPFGFQPSELFRFAFILFLSYSLSKRLEKIKEFKYLFIPYFPLLAVGFLLIIKEPDL
                     GTALTIGLTGILLLFIAGARLRHLSLVILPALAVVPFLVFGLGYEKERMDDYFKSLKD
                     PLAGSYQLTQSVLSLGSGGLWGVGLGEGKQKLLFLPEPHTDFILANMGEEGGFALLAA
                     TLILFLILGWRGFKIAFKAQDYFGFFLAFGITSAVMFGFIMNACVISGILPTTGLPLP
                     FLSYGGTSLFLNLLAIGVLLNISRQRHNCKESKVVGKSSLW"
     gene            complement(21914..23308)
                     /gene="murD"
                     /locus_tag="VGB16_06805"
     CDS             complement(21914..23308)
                     /gene="murD"
                     /locus_tag="VGB16_06805"
                     /EC_number="6.3.2.9"
                     /inference="COORDINATES: protein motif:HMM:TIGR01087.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008764 -
                     UDP-N-acetylmuramoylalanine-D-glutamate ligase activity
                     [Evidence IEA];
                     GO_process: GO:0009252 - peptidoglycan biosynthetic
                     process [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="UDP-N-acetylmuramoyl-L-alanine--D-glutamate
                     ligase"
                     /protein_id="HEX9917432.1"
                     /translation="MIQILSGERKVGKREDRVFDRKISVLGMARSGIATAQLLSQKGA
                     EVFVSDIKSERELENEIAKIKSLNINFEVGAHTDRVLEDKDFIVTSPGIPSDIPILKK
                     AESLGIPIFSEIEIAFWLSQSPIVAITGSNGKTTTTYLVGEILRQDQKEYRVAGNVGV
                     PFSSVVDEVSDKGIIVLEVSSFQLERIEEFRPKVASILNITPDHLDRYPDLKSYQEAK
                     VRLLENQIPQDYTVLNADDLVSSSLSPCTQAKQIYFSIKKDMEEGVLVRGENLVLKSK
                     KGETAILPIKEITIKGPHNLSNAAAACAMAYALEIDTASMARALKEFKGVEHRLEYVA
                     TINGIKFINDSKATNVDSVWYALQSIDEPIILIAGGKDKGGDFTRLGELVKSKVKKLI
                     LIGQAKEKIKRELGGLVDTIEAETLEQAVKLGFEKAQEGDVILLSPACASFDMFKDFE
                     ERGRKFKLAVHNLK"
     gene            complement(23305..24393)
                     /gene="mraY"
                     /locus_tag="VGB16_06810"
     CDS             complement(23305..24393)
                     /gene="mraY"
                     /locus_tag="VGB16_06810"
                     /EC_number="2.7.8.13"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011737102.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008963 -
                     phospho-N-acetylmuramoyl-pentapeptide-transferase activity
                     [Evidence IEA];
                     GO_process: GO:0009252 - peptidoglycan biosynthetic
                     process [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="phospho-N-acetylmuramoyl-pentapeptide-
                     transferase"
                     /protein_id="HEX9917433.1"
                     /translation="MLYHLLYPLSQNISFFNIFRYITFRTAYASVTALLLSLIFGPMI
                     IRILKTKQVTENIRADGPATHMAKEGTPTMGGIIILLSVVVPTALWADLANRYIQVIL
                     LATIWMGLIGFMDDYLKAIKRVPKGMVGRKKLMGQISLGLVLGLWLLLEPPSKNFGTS
                     TDILFFKNYFLDFGILYIPLVILVITGSSNAVNLADGLDGLAIGLVGICAFAFAVLSY
                     IAGRVDFSRYLSVVYLQGSGELVVYCGALMGAALGFLWFNAYPAKVFMGDTGALALGG
                     ALGVISILIKKEMLLLIAGGVFVIEALSVIIQVVYFRATGGKRIFRMAPLHHHFELSG
                     WKEPQVVVRFWILGVIFALLALSTLKIR"
     gene            complement(24394..25791)
                     /gene="murF"
                     /locus_tag="VGB16_06815"
     CDS             complement(24394..25791)
                     /gene="murF"
                     /locus_tag="VGB16_06815"
                     /EC_number="6.3.2.10"
                     /inference="COORDINATES: protein motif:HMM:TIGR01143.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_function: GO:0047480 -
                     UDP-N-acetylmuramoyl-tripeptide-D-alanyl-D-alanine ligase
                     activity [Evidence IEA];
                     GO_process: GO:0009252 - peptidoglycan biosynthetic
                     process [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="UDP-N-acetylmuramoyl-tripeptide--D-alanyl-D-
                     alanine ligase"
                     /protein_id="HEX9917434.1"
                     /translation="MIEISINELLKVSNGKLLKDRIASNKKMKGVSIDSRTLQKGNLF
                     IAIRGENQDGHKFIPQAFEKGAILAVAEKKRVGDINKEHLPIILVGDTKKTLYDLAWW
                     YRRKFNLPTVGITGTNGKTTTKEMVGAVLSEKYKVLRSPKSYNNLIGIPLTLFQIELQ
                     TEILVLELGMNSLGEIAQLTKLTQPDWGVITNIGPAHLEFMKSPENIAKAKFELADNM
                     AKDKTLVLNADDIFLNRRIKEEEKRVITFGIEKQADFRATSIESNGSGHYHFKVNSQP
                     QIRLKVLGRHNIYNALAAFALAKELGIDEDLIKQRLENYKPAQLRMEWFKVRGITLIN
                     DAYNANPISTQNALETLDQIKSQGRKIAVLGDMLELGEKSDEFHFNIGEQVGQLNLDK
                     LLLRGEFSESVAAGATGKGMDKTKIEIFKENKKISSWLLDNLKTGDMVLFKASRKMRF
                     EEIVEELNNFLNRQN"
     gene            complement(25784..27262)
                     /locus_tag="VGB16_06820"
     CDS             complement(25784..27262)
                     /locus_tag="VGB16_06820"
                     /EC_number="6.3.2.13"
                     /inference="COORDINATES: protein motif:HMM:NF001126.0"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_function: GO:0016874 - ligase activity [Evidence IEA];
                     GO_process: GO:0008360 - regulation of cell shape
                     [Evidence IEA];
                     GO_process: GO:0009058 - biosynthetic process [Evidence
                     IEA];
                     GO_process: GO:0051301 - cell division [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="UDP-N-acetylmuramoyl-L-alanyl-D-glutamate--2,
                     6-diaminopimelate ligase"
                     /protein_id="HEX9917435.1"
                     /translation="MGVPLRKLIESMNTKKTYGDTDFDINKIEYNSTKIKPGDLFVAI
                     KGFQTDGHNFINDAIKNGAICVVGEKDQDHNIRAEILVKDSRMVLAFLSDKFYDSPSR
                     KLKVIGITGTNGKTTVSYLLKSILETKERKIGLIGTIAHYIGDQQVPALNTTPESLDL
                     QRMFYQMLEQGIKYVVMEVSSHSLALKRVEKVDFDVAVFTNLTQDHLDFHHDMESYKK
                     TKGKLFEMLVKPEDRAVINLDDPNWKYFSKKTKAPKVTYSLRNKEADVFVEKMNHDLK
                     GLEIRSSTPLGEFEVRLNFIGEQNVYNALAAIASSVALGFELENIRSGLEKARVVSGR
                     LEKINLGQPFDILIDYAHTPDALERVLITAKELTSGKLIALFGCGGDRDKTKRPIMGK
                     LATRIADYVIVTSDNPRTENPSSIIDDIFKGINSKNDIKRIEDRKEAIKQAIDLAKPR
                     DILVIAGKGHEDYQIIGTKRFHFSDKEAVEEILKGMGYKGND"
     gene            complement(27266..29227)
                     /locus_tag="VGB16_06825"
     CDS             complement(27266..29227)
                     /locus_tag="VGB16_06825"
                     /inference="COORDINATES: protein motif:HMM:NF013100.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008658 - penicillin binding [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="penicillin-binding transpeptidase
                     domain-containing protein"
                     /protein_id="HEX9917436.1"
                     /translation="MTYKVPQGRFYLILMLGLLGWFILILRLFQIQVVQGKIYQKISD
                     RQHQLCLEVSPQRGAIYDRNLQVLAFNLPSESFFAIPESLSDIYSVAEKFSSITSFTP
                     QEITKDLKHYKKFAWLKRKVNREESELIRSWNLVGVYSKKESKRVYPFGDLADEVLGF
                     ANIDDQGAAGVEYQYDQTLRGKTGQILINRDAKMRNFALKAEPLVKCENGNSLVLTLD
                     LRVQAIVEEELRKAIESNQADGGTSVWMDPKTGEILAMAHFPKNGNSPSLKTKSVSDL
                     FEPGSTFKIVTAVAALEEKVKNPEDSIYAEKGEFEVNNHKIHDVHKYEWLTFRQCTAY
                     SSNIAFAKVALQVGKNKLYRYARNLGFGIKTGVDLPSESKGAFPSLRKCSDFVLSTLA
                     FGQGVSLNSLQLVNAYAAVANGGYLMKPYVVKAILNQEGDTVEEFHPQKIRHAISPRT
                     CQIMNDLFRVVVDSGTGYPAKVEGLDIAGKTGTAQKPDSINGGYKRGEYLSSFIGYFP
                     ASQPKFVGLVCLDNPQGKYYGSETAAPAFRNMAQRLANLQDEPLVLVKTTTSEKNIIS
                     QTSTGKPKGKQVGGMPIMLGENLADASTDGIVVPNALGMTVRQAAHLFSKEKINFKII
                     GSGVVKNQTPSVGSRLNPGQICLLHCETE"
     gene            complement(29217..29633)
                     /gene="ftsL"
                     /locus_tag="VGB16_06830"
     CDS             complement(29217..29633)
                     /gene="ftsL"
                     /locus_tag="VGB16_06830"
                     /inference="COORDINATES: protein motif:HMM:NF039650.3"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0016020 - membrane [Evidence IEA];
                     GO_process: GO:0000910 - cytokinesis [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="cell division protein FtsL"
                     /protein_id="HEX9917437.1"
                     /translation="MRKKFKRKISSDTSFPYFRIVLILLLVVGLALVYVWQRVCVIES
                     AQEIQTLKNQLSSCKAEYKYLSLDVTKLGSAQNIERIAQSELGMATYDQPISFLVEPK
                     TSEKPTSLLSKAKQAVEKMTNISENQVEAGEVKHDL"
     gene            complement(29643..30566)
                     /gene="rsmH"
                     /locus_tag="VGB16_06835"
     CDS             complement(29643..30566)
                     /gene="rsmH"
                     /locus_tag="VGB16_06835"
                     /EC_number="2.1.1.199"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011916477.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016434 - rRNA (cytosine)
                     methyltransferase activity [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="16S rRNA (cytosine(1402)-N(4))-methyltransferase
                     RsmH"
                     /protein_id="HEX9917438.1"
                     /translation="MVHIPVLLSEVTEYLIVDRSGIYVDATVGEGGHAYAILSGLDQR
                     GKLICTDRDEQAIRKAKSNLAEFGKQVVFQKLYFSQLEDFLKELKIDNLSGVLFDLGI
                     SSAQIENVHRGFSYLSDGPLDMRMDQAQSKTAADVVNSYDPKELARIFFELGEERFSR
                     RIASAIVRARKKREIRTTGHLREIIENKVNPKHKIKSLARIFQALRIEINQELEELKR
                     GLKTAVDFLKPEGRVCVITYHSLEDRIVKNQFRRLSQTCNCPPYFPICRCGQKQVLRV
                     LTKKPIVPSLEEIKANPKSRSAKLRVAAKLG"
     gene            complement(30580..31020)
                     /gene="mraZ"
                     /locus_tag="VGB16_06840"
     CDS             complement(30580..31020)
                     /gene="mraZ"
                     /locus_tag="VGB16_06840"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_007505626.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0043565 - sequence-specific DNA binding
                     [Evidence IEA];
                     GO_process: GO:0051302 - regulation of cell division
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="division/cell wall cluster transcriptional
                     repressor MraZ"
                     /protein_id="HEX9917439.1"
                     /translation="MATFKGFYRHTVDHKGRINIPAKFRKTGTSTLHESFVITRGLEG
                     CLFLYPTEEWQRIDEKLRSLPFTQSHNRYFTRLLLSNASDVQVDKQGRIAIPQPLMEL
                     SQIKKEVLVLGVLERIEIWSPEVYQKYLDGFEQSYEEVAEKTLL"
     gene            31382..>33587
                     /locus_tag="VGB16_06845"
     CDS             31382..>33587
                     /locus_tag="VGB16_06845"
                     /inference="COORDINATES: protein motif:HMM:NF013524.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008234 - cysteine-type peptidase activity
                     [Evidence IEA];
                     GO_process: GO:0006508 - proteolysis [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="C25 family cysteine peptidase"
                     /protein_id="HEX9917440.1"
                     /translation="MRFLRFFFFIFFVPALCVADITKEIHFDIGDLTRSEQDGKIYIN
                     LKDSQREFRVGYPDLPIKLLTFIIPSDVEVDSLEVSSLSSDTLEEEYTISLVGADTKT
                     DDNSAIEYQENDTLLYNPDLPYPQKLASILNCGYLSGNHLITLTVYPLQYFAAGKRLV
                     FHNDLRIKLIFKPNQNNSNSFLMNKKVNQGILEELVFNKEDILSCSPAVSLSKQISIP
                     ASAQYIVITNQEMKDALVPLIEWKTKKGIKTEIVTVDSIYSYYAGMDFAEKIRNFLIE
                     AYQRGLEWVLLAGNEDIVPVRYAYQSNASTPPSIANQQICDLYYSDLTGNWDQDGDGI
                     WGEPLDDSPDIYPEIFVGRVPCQNAQEAGNFVEKVLNYERNPIEGGSDYLNRALWICS
                     DQMRDWNEEEGQHKLISRYIPTNFYQELNNLIESPSGDAPDPVSPKGQNCIESMNQGW
                     GIIGILAHGRIDGFVASSNLINEWPKSYVFSWPAEEDGHGHLTSLSNQGKYGIVYSIS
                     CNQGAIDYDKELTLGGGTSVAESFILSCQKGAVAFLGYSRWGWVSSSYKLGQAFMQEL
                     FDSTNDYHLGMAEALSKLYYPYYWDLNYGHNLFGDPEMQVWTQVPGSFSVSYTQTIET
                     GSEDAEFQVSTNGNPVPQAKICITEGNEILFLAETDEQGKLSAQLDIDHCTELAVTIT
                     KTNFTPYQGTIKVSSTAGVGENQDPVPLSFELSQNYPNPFNPTTQINYQIPED"
BASE COUNT         9420 a         7601 c         6858 g         9708 t
ORIGIN      
        1 gtgtcgaagg aaggctcagg ctcgaagaga tggtgaggac attcggctga gcggttcgac
       61 cgtcctgagc ggctcaccgt cgaaggactc agtcgaagcc catatcgaac catcaaatcc
      121 ccctttttca aagggggaga cttctttttt cccctctttg gaaaagaggc ccgcttcgcc
      181 cgtctcgccg atggctcgcc gaggcgggcc gaatcgaggc gagggctagg ggagatttat
      241 tgaacgtaag ttaatgtgtt tgtattagag agaattcaac cttgactttt tttgcctgga
      301 aaggagataa atatgcaaaa gttgggtttc aagacctggt caagccttct agtggcaagc
      361 ttgattggag tggttttggg gagtgttgca gtgatggtgc ttttcgctaa agtttttccg
      421 ttgaatgtct cacttgatca aaaaccgagt ttttacgaag gagctaaaag gataaatgtc
      481 ctgtccacca cagaggaaaa catcacttcc acacgggaga atgccatcgt tagggcggcc
      541 aaaagagttg gtccggcagt ggtatccata agtgtagtgc aaactagaat catacgcgcc
      601 accccttact cctccccttt tggagacccg tttttcgatg agttctgggg tcagttcttt
      661 ggctcgcgtg agtataagca gaagatctat ggattgggct ccggagtaat aattaatccg
      721 gacggctaca tcttgaccaa ccagcatgtg gtgcaggatg cagatgaagt taagattacc
      781 ctaaccaaca gtgaggagta ttcaggtgaa gtggttggcc aggatgtaac ttctgacctg
      841 gctttggtga aaataaaggc aaaaagctta ccttacgctg ccctgggaaa ttcagatgat
      901 ttaatgattg gagagtgggc cattgcttta ggaaatcctt ttggttatct cttgaatgat
      961 gctaatccca cagttaccgt aggggtgata agtgctctcg gtcgtgatat caaaagagaa
     1021 aggggacagt tgcaagttta tcgcaatatg attcagaccg atgccgccat aaatcctggc
     1081 aacagtggtg ggcctttagt gaacgcagat ggagaggtaa ttggaattaa cactttcatc
     1141 ttcaccacca gtcgtggctc ggagggggtt ggatttgcca ttcccataag ccgagccaag
     1201 gggattatcg ctgacttaat caagtatggc gaagtgaaaa aagcttggat tggaatagaa
     1261 tcccagagga caagctcatc ctcggggcag gatgtaaatc gagaaaaaaa tagaggagtg
     1321 gttatcgctc aggtggataa ggatagtcca gctgaaaagg caggtttaaa gaaaggggat
     1381 atcataaccc aaatcggaac gcaaaacata aataaccaaa gcgactggga tgaaatagtt
     1441 tccttggcta aggcaggcga gactttgaat gtagcctatc tgcgtgaagg gaaagaaatg
     1501 aaaaccgaca ttatctgtga gcccttgccc gcggttagcg ttcaaaggtt tgatgacaat
     1561 tcgggaatga ccttagcttc cataactccg agcttagcca gtcaattggg aacagataac
     1621 caaaagggag tagtcatagt tgaggttaaa catggaagcc aagccgatta tttgggttta
     1681 gagccaggag atataatccg tcaaatggaa aattatacta ttgatgacct atccaaatac
     1741 aagagtataa tgaaagacct tacaaacaga aaagttgctc tgctggtgga aagagaggga
     1801 gaagtctact acgtctcggt aacgaggtaa tgagcgaatt cctttaggac aaaaacaaaa
     1861 gccacctaac tcaaaggtgg cttttttgaa attaaagttt gatttttgac ttacttcaac
     1921 aacagcatct tcttcgactg gacaaaccct tctgcttcta tcctgtagaa atagattccg
     1981 cttgagacaa tctctccatt ttggttcttg ccatcccaga ttactctcct aaatccagca
     2041 ggctctactt tgtcaagcaa ggttgttact ttttgcccaa ggatattata aatggctatc
     2101 ttcacttggc ttgccttagg cagggagtat tctatcactg tctcggagtt aaaggggttg
     2161 ggatagttct gcgataggat aaaggagcta ggtttggcgt cacctatctc ttcccctaca
     2221 taagaagcat tcgtctgatt gaagagaatg gaaacattgt tgcttgcacc atttgccacc
     2281 gctaagtcca gatccaaatc accatccagg tctgcacaga agacagaata agggtcaacc
     2341 ccaacaccat agttaacctt gccttcgaat gttccatctc cattgttcct taagatagaa
     2401 atgctgttgc taccactatt tgccactgcc aagtcgaaat ccaaatcccc atccaaatct
     2461 gcagaaaaca cagaaatagg ctttatcccc acaccatagt caaccttagt ctcgaacgtt
     2521 ccgtctccat tattctttag gatggagaca ttgccggtag tataatttgc cactgccaag
     2581 tccagatcac tatccccatc cacgtcagca cagaaaacgg agcgagggcc gcttcctgca
     2641 gcatagtcaa ccttagtttg gaatgttcca tctccatcgt tccttaagat agaaatgctg
     2701 ttgctaccac tatttgccac tgctaagtcc agatcaccat ccccatccag gtctgcacag
     2761 aaaacagatt gggcgtagaa cgttacatca tagtcaacct tagtttggag cgttccctct
     2821 ccattgttct ttaggatgga aacgttgccg ctaatagcgt ttgccaccgc taagtccaga
     2881 tcactatccc catccaggtc agcacagaaa agggagcgag ggctgcttcc tacagcatag
     2941 tctatccgtg cgtcaaaaag aggaacaaaa ggttgaactg tgatggcgct tatttcacga
     3001 gaatgatcac tttcctggaa gtttgtatcc acattggtga tggtgtaata ataagtttgc
     3061 cctgaatcca gtccaaggtc ttggtattgg ctatcaggtt gaggaattaa gttagtattt
     3121 atcgtgtctc ccggctctcc agaggtagtt gccctatata tgttatatcc cagactggcc
     3181 caagaaggtt tcggattcca ggctaaggtt acactgctat cgttggagct tttaacatgg
     3241 aagttgtaag gagggagatt aactatttct ccttctgttg gcttaaaccc atatcttcca
     3301 ctattccact tatcgtgcaa gtacttgggc caaggcatag tcgtggaatc ataacagtta
     3361 ataaacttcc aacacgtcag tttgggatcc tctgtaccca aaaccatatc tagctttcca
     3421 tctttgtcca cgtcggccat tatgggagag taagagcaga atgtaaatga acgttcctga
     3481 gctgatgcat aaagtggcca accgtctatt tctcttccgt catagtccca agcaaacacc
     3541 ctttcatacc cactgccgaa aacatagcct gctcgggcga ggatatctac gtttccatct
     3601 tcattcacat ccgctaagac tggacttcca aagctctcag ccacagaggc aaaaacaggg
     3661 tcttggtaaa gactggaagc aagggagttt aattcctctt tactcatgtt cttaaactta
     3721 gtcaatacct cacgtaagtt attgcccaag gaagataggg cttgtcttat tttctcaccg
     3781 ccaggtgtct tcatggatac cagagaccct ccagggccaa ctataccaag cggtgttcca
     3841 tcctgatgcc aggcatagat gcgagcctca cccaatgtcc aaacacttac cactatctct
     3901 aaaattccat ctccatccaa atctccaata gctggaggat tcccgagcca gttacccgat
     3961 atcaaatcct ctattattat gggatagccg ctaacttgag tgccatcctt tttgaaggcg
     4021 gaaagggcag catattcccc attttctcca aatgttacta tctctaagtc ctcattttca
     4081 tctacgttgg caatagcaat tccaaagacg gcgccgaaga tgtcaggata atccccaggt
     4141 ccgagtatca agtttccttc tatgtcccag atatagatcc cgccaaaata cataggatag
     4201 tcattccaat ctgccgctcc aacaattacc tcattcttgc catccctatc caagtcagtt
     4261 actgttaagc agggggaacc ttccccactg ctcatcaccg cttgagcaaa gactccatca
     4321 tttccaggta tcactggctg gccatccttg tgccaagcat agacgatccc caaagtgtcg
     4381 tttatagtta gtacttcgag gtaaccgtcg ttttctaaat ctgccagcac tggtgtagca
     4441 aaactcaaca ttgcgacctt tcctcctgta ttcgcacttc tgaaccagct gggagaactg
     4501 gaaagataag catgcacccc cagattggag cagataacta tatcatctat tccgtcatta
     4561 tccaggtctc ctaaagcagg ggaggaggac atatagtagt taatatcctt gggccagcca
     4621 gcttctcttt gaccatcgtg gctgaagacc tcaactcctc caggtgttcc aattcctccc
     4681 atacttccca ctcctaccac aatctcatca tacccatctc catcaatatc tccaattgca
     4741 ggggagaggg agccataact tgagaggtct tggatccagc cgccagaggt aatgcttttt
     4801 acaaatatag atatactatc agcataagtc tggtcagtat ttgaatgtgc agttaacctc
     4861 aaggtataat taccttcatt cagaaagctg gttagccagt tccccaggat ataatccgcc
     4921 actatcttgg tggagctatc tatgggagtc caactggaag gtgattctcc ttgtccatat
     4981 tctaaggtgt aataagagaa gccagggata atggtgtaac cagagatttg ggtgtatcct
     5041 tggatagtat caccctcttg gggtgaactt atcttaacat agacgctact attgactatg
     5101 acgtgcacaa ctacttggtt ttggtctccc acagtcagtc ttatactata aagaccagtc
     5161 aagccagaag cattaaatat ccccaaggtg tctttggcta ctggggtagt ggagcttgtt
     5221 atctgggtcc agctttgtgg agaagcacct tcaccatatt ctaagatata gctcacaaag
     5281 tttgctccac tggctgttcc tatgatagca acttctcctg agaccactgt attctcataa
     5341 ggataatcta tcttggatag ccttccagag attaaagcca aggcagagtt aagatttact
     5401 ctgccataac cactgtagat gtcctttcca ggagaagata gactatctcc tccataagga
     5461 tagatgatat catctgcgga ctgttccatt atgcttatta cactatcatt acttatccca
     5521 ggtgaagctg acaagatata agctgccact cccactgcac agggagaagc catagaggtg
     5581 ccgtcagcca gataatactt ttcatctatg atatgggtaa gtgggtatcc attctcctca
     5641 tacatatccg tattatccgc tcttaaagag agtatatcct cacctggtgc caccacctct
     5701 atttgattcc cataagtgga aaagtaagtc acctcatctt tactattgga agctcctaca
     5761 gccaagacct gaggaaggga agcagggtag aggttatctt ctgctccact atttcctgca
     5821 gctgcgattg gtaggactcc tttagagaca gcataatcta tagcatcctc caccacagat
     5881 gaaggatagg gtgctcccca gctcatgttt atcacatcac atcccatatc tgctgcatag
     5941 atgatactct ttgctcccaa ggacatgaat gcattgggga agaacttaat tgccattatc
     6001 ctgcaagggg tatttatgcc tgagactcca atgccattgt tccttactgc tgcaacaata
     6061 ccagcacagt gagttccatg accaaaatag tcagtggggt cgttatcctc tatcatttca
     6121 tcgtcattac ctgaaaagtc ccacccatag aagtcatcca caaatccatt atggtcatca
     6181 tctgtaccat taacttcaga tggctctatg ataccattac catttaaatc ctctcctgga
     6241 ttaacccaga tgttatcctt taagtcttca tggtctaaat caactccagt atctattatt
     6301 ccaactaagg gaataacttt ctcatctgct ctttggaatg cttctaaggc atctatgtct
     6361 gcatcagttg ttccgaactt cataactaac gtatgtcctg caggtctgtt gattccataa
     6421 tacccttgac cttcattctg ggcagctcct gtgttatcca gataccactg gtgagcaaag
     6481 agtgggtcat tgggctgttc aaatagctct actttataat caagctccac atactccact
     6541 tcagctcttc tctcatactc ttccttcatc ttccttaagt ctgttccttc tggaacttct
     6601 aaggtataga tgctggagaa tctacctacc tttaaagcag tttttttaaa ctccttaaac
     6661 agtctttcct gcttccttac atcaaatcta acattaagac tatccaaaaa agcaactcca
     6721 gtaactacgt ttccactaac cttacccaag cttactttct tatcaacttc cggctttagc
     6781 ttcacaatca gtcgggtagg agagaactgg ggttgggctt gttttgtctt gccagagtag
     6841 atagagtaag aactctgggg aagataaaga aggataaaaa ggaagataag aagaaaaaat
     6901 aggctttgtt tcttcacaga tcgccccttt ttgtttacag ttgttagtta taatatcgac
     6961 tcttaatacc ttacctttaa aaaagagcaa aaacaatgcc aaatagttgg ggccaagtaa
     7021 ttagaggttt tcctattttg atttgttggc tacaatgagt tgcggttaat caaagaatcg
     7081 gaattttaga ggacaatcaa gagggtgcgc aagagcttgc gaagcctgcc tggatgttct
     7141 ttgagtttat agtatatcct atttaaacat caataggtta gaagatccgc cagaaatggc
     7201 ggatatttac aaggaatggc gagtctattt aaggtcttcc gttaagataa aaatgtcttg
     7261 ctaactctgg ttttgtagct aaattataac taaaatcgaa aaagaggaga aaccataatg
     7321 aataggttgt tcaaaacaac agcgttaagt attatcctta ttttgggtct gggatgcagt
     7381 ttcgccaaat atgacctcaa tcgaattcgc cagcaggatg aagctttcac caaagagata
     7441 atgctttcca gttatgaaac tgatgcttat ggcaaggaat cgcatatcgc cgaaatctat
     7501 gggaagcatg aaaagctatt cacagatgag aatttagtcc gttttctcaa agataggatt
     7561 gaaaaggaac gcaatcctcg cgagaggaaa aaggcagaat ttctgtatcg tgacatttcc
     7621 agtagctttt tgggagagag gaccaaggag ttagatgacc ggatatttga tctgcaagcc
     7681 aaagagaaga tagtagttga gaaagacacc attgcctttc gggacataga cgttctgctt
     7741 tctaacgaga aaaacggaga aagaagaaaa gccctttatt attctcaagg ggagatgata
     7801 atagataaaa taaatcctct gttaactcaa gagctgaaca tcctggagaa aactgtcaag
     7861 gaattaggat acagaaacta ctctgattat caacaacagg tgagaggtat cgattttgat
     7921 tccctggaag aaaactgcag ggagatatta aaggaaactg ctccgattta taaagccctc
     7981 ctatctgaga tggcaagaaa gattctgaac aagagaatgg aagacgtcaa agtttatgac
     8041 cgggcatggc ttttcagagg ggaaagtttc gatgaatatt ttcctaaaga gaaaatggtc
     8101 ccgattttca agtcaactat gctggatatg ggaattgatg tgggtgccca gaagaatatt
     8161 agtttggatg atgaggatag gccggaaaaa gaacccaggc cagccaccta cacaattgtt
     8221 gttccaactg atgtgcgggt tttggtcaag ccgatgggtg gatatagcga ttacggttca
     8281 ctttttcacg agatgggaca tgcagagcat tcggcctaca caagagtcag cgaatttgaa
     8341 ttcaaggatt taggggatta tggagtaacg gagatgttcg cctatatatt ggaggggata
     8401 gtttcaaatc caatttttat gaaagataag ctgggaatga gccagaagga tatcaaagaa
     8461 ttcaccaaga tgaacttgtt ctatagcctc tcctctttga gaagttactg tggtaaattc
     8521 attttcgaaa gacagcttca ttctggaaat cccgacccca ttaaagtata tcaggaaaca
     8581 agcaaggaag cattgctttt ggatagaagc cataaagagg cggagatgat atatctttta
     8641 tacggggatg aaagatttta cagcgtaaat tatcttgagg cttggatttt ggatgcgcag
     8701 ttgagagcca aacttaagga gagattcggg gagaattggt ttgagaaaaa agaagctggg
     8761 gagtatctcg aatccctttg gacaaccggt atggagtaca cctccccgga aatggctaag
     8821 ttgattaaat atgataagat ggacccaagt tatctggtca aagacataaa acagttgtac
     8881 gattcagtaa aataaaacta aaaggagtga aagatggcca aggtatgtga tatttgcgga
     8941 aagaaaccgc tttatggaaa caaagtcagc cacgccaata accgcacagc cagaaggtgg
     9001 atgcccaatc tgcaaagggt tagagctttg gtgaacggca aagagaaagt catcagcgtt
     9061 tgcaccagtt gtattaaagc gggcaaggtg cagaaggtta agtagaacat agatgttttt
     9121 gtaaagtgat tttggttgtg gtgttaattc gtcttttagt aaaccataac cgaggaggta
     9181 cattatgcta cttagaacca ctcttatctt tctaactagc ttatttcttg cctctggtct
     9241 taatgcccaa gtttgtgtca attgtcacaa aagtattacg cccaaaatcg tctctgactg
     9301 gcaaataagt aaacacagtc agaatggtgt ggattgttca gtatgtcacg ggaataaaca
     9361 cacatcaccg aaaaatgtag cccaagccga aattcccaca ccagagacct gcgctgcctg
     9421 tcatcaaaca caagttgacg agttcaaagc cgggaaacat tctctcggct gggcttcgat
     9481 gaaagctatg cccactgccc actggcagcc catggctttg atggaaggga tgaaggggtg
     9541 tggtggatgt cataaaattg gattaaaaac agaagcggag ataaatgagt tgaagaaaag
     9601 tggtgcagga tttggcttgg cttcctgtga tgcttgtcat acccgacata tcttttctgc
     9661 agttgaagcc agacagcccc aggcttgtca gacctgccat atggggtttg accatcctca
     9721 gtgggagatg tattcttcct ccaagcatgg cgttagatat ttgctaaaac aaagcaagac
     9781 cctcccggaa acagtttccg cacctacttg ccagacttgt catatgcaga aaaacaatca
     9841 tgctgtcagg actgcttggg gatttttggc agtccggctt cccttacctg aagataagaa
     9901 atgggcagca gaccgtatga tgattttgca gggcttggac gtattaggtt tagatgggta
     9961 tcccactcct cgattggaag tagttaaagc ggcagatatt gtgcggacaa cccaggaaga
    10021 ttggcaaaaa gaaagagaca agatgataaa gacttgcagc cagtgccatt cagtcaattt
    10081 tgccaaagag gaattggcta aaggggatga gatgataaag cagtcggatg ggcttatggc
    10141 tgaagcgata cacgtagttg cagacttata taaggatagc atcatcacaa aaccgcaaaa
    10201 ttatgcttat caatttcctg atttattgac cttccacgat gcccccactg tgattgagca
    10261 gaaacttttt gtgatgtttt ctgaacatag aatgagaact ttccaggggg ttttccatag
    10321 caatccggat tacaccttct ggtatggttg gagtgaaatg caaagggatt taacagaaat
    10381 aaagacgatg gctgaggagt tgcgaagaaa ctataagaat aaataaaata aggattaaac
    10441 caaccagggg gtgtaaatga atagagtcaa agatagagta gctatcgtga caggtggcgc
    10501 attgggaatt ggccaagccg cctgtttgct tttagcaaag gagggtgcca gggttgcggt
    10561 gacggacatt ttagataaag agggagaaga agttgttagg aatatcaaaa gcaaaggggg
    10621 tgtggctgga ttctggcata tggatgtttc caatgaaaag gaagtgcaaa aagtatttgc
    10681 ggatgttgat aagcagtttg gaaaacttga tattttagtc aataacgctg gaatatccgg
    10741 gccaagcaag cccacagacg aattgaccga agaggaatgg gacagggtta tggcgattaa
    10801 tgttaaaggt gttttcttct gcaccaagca cgttattcct tatatgagaa aagcaaaagg
    10861 cggaagcata atcaacttat catctatata tggaatcatc agcgcaccgg atgtgccgcc
    10921 ctatcacgcc tccaaagggg ctgtgcgcat aatgaccaaa acagatgctt tcctttatgc
    10981 caaggacaaa atccgagtca actccgttca tcctggtttc atctggactc ctatggtgga
    11041 aaattacgct aagtcccagg gtaatgtgga agaggtaaga aaatatctag atagtctgca
    11101 tcccatcggc catgtgggcg aaccagatga tatcgcttac ggcatccttt atttagcatc
    11161 agatgaatcc aaattcatca ccggtagtga attggtgatt gatggaggat atacggcgag
    11221 gtaaaggcaa gagataaaat attttcaaaa acaaaacaat gcgtgcagaa cataggaata
    11281 ttcagaagtg gataaaagct cgctatggaa ataagtataa cgtcgaggcg gaaattgatg
    11341 gacagaatta ccttccagat agagtgcggc attggtacca accagatgtg gttttacgaa
    11401 gcagcaaaga tgaaattaaa tgtataattg aagttgaaaa caatccaatg agaaaagcac
    11461 tggtcggtgc ttcggttctt gctgattgct ctgtcgctgc actaaagcaa caaaataaac
    11521 ctcggctgat tttcgtagtt tatacgaaga taggcataaa acagattcca aactttaagc
    11581 agaagttgaa aatcacgaag aaatattatc tacatctgaa aagtattaga atatattcgg
    11641 agttagattt caaggcatta caattatgat taagctacgc gtaggggtgg agtttatctc
    11701 caccccttcc gttcgggcga agacgagctt cgcccctacg gtatagatta tatggtttag
    11761 acctacgcga tttagtattt tgtaggggtc gcatttatcc cgataaatcg ggacaagttg
    11821 tgacccgaaa accaaacggg ttcgataaat cgaaccccta cagtaaactt gtttgtcatt
    11881 cccgccccgc atttctacgg ggtaaactcc agcgggaatc catttttaaa agccagtaga
    11941 ttcctgcctg cgcaggaatg acaatgatct aacctaagac aaatccttta acaagaagga
    12001 ggagaaatga gttcgtctga taagaaaaag atagtagcga taatgaaggg aaactcttcg
    12061 catgcctatc ttgctacctg tgacggagac cagccgaaag ttcgctctat gtccacgata
    12121 gttgaggacg atatgaatat ctggatggcg acttccagca attcaagaaa ggtaaagcag
    12181 ataaagaaaa atccgaaagt atgtctggct tttgtgaaac aaccaaatgg agataaatcc
    12241 gcttttgtaa tgggcaaggt caagataatc aattccttga aagacaagaa gagagtatgg
    12301 aaactttcgg gttatgatat gtgtcaatat ttcaaagatg gtccccagtc caaagagttc
    12361 tgtcttttga aggttatccc caagaaaatc gagtggtggg atagttggaa aagtggaaga
    12421 aaaatctata aaccaaagaa aaagcaagtt aaaagtataa actaataaaa cataaagggg
    12481 gcgaaaaaga tgcagaggtt gttattaaaa gctttttgtt tcctgtttat atttttctta
    12541 attgttttgt ataatgtaac acctgtgatt gcccaagtga agtcagactg ctcgtttcct
    12601 aagttgcttt tagtcggtta caataaagag ggaagtaaat tgggagactt gtatcagcaa
    12661 aaacagactc gattcaaatt aaaatcggaa aatgtaggtc tatgttattc tttgttgggc
    12721 acgtttgttc caattggtac cggtatacta atttggattc tggatgaacc ctattatgtg
    12781 gaacatgata ggccggaccg gtatggagga gactggttag agaaaaaaga cccaagtcgc
    12841 gtcattcctg ttgcattgat cgcatccgga cttatagtcg gaccatcaat gggatatttt
    12901 tatgctggag aaagttggcg aggttttaag ggtgtggcaa ttagattggg aacaggagga
    12961 attacagtat tatgcatggc actttgttac gattcggatt tagataatta tgcgacattg
    13021 tttgggggca ttggtttcat tagttcaatt gtttttctga ttgatgtcat ctacgacata
    13081 gctaaggtta aaagcacggt ccgaaaacat aatctcgcac tacacaaaac agctttgatt
    13141 ttggcaccga gatattttgt tgattcaaaa gcatgcggtg tcggattgca aattaagttt
    13201 tagtcagatc tattgaagtt aaagaagaat acgggtggag atacggttcg accttgctta
    13261 ccgtcctgag caaagtcgaa ggaaactcca cccctacgcg agcaatcaat tcgcctaatg
    13321 aatcccgata aatcccgcca gaggcgggat aaatcggcac aggtttgggc aactgcagta
    13381 aggtcaaagt agggacagat tataatctgt ccctacgcta ttataattcc ttagcaggcg
    13441 acccgggggg tcgcccctac aaaatcatat actcttagcc cttttatatt ctctcaaaag
    13501 gaactgttta ggaatttgat gctcgactat ctcccagggg aaaacgccat cctctttctt
    13561 ctcctcaaat agaagatgct ctatatcaaa ccttctttgc ttcaagaaag agtatatatt
    13621 catcccttcg gtctgctcgt gatacaatcc ttcccctacg attctatttc ccaatgaaag
    13681 aagggcttgt agataaaacc ctttaatgcc tttaccttga aatttaatcc cccttaattt
    13741 cgatatcccc ccttttattt gaatatactt ttgttccaat tcctcttttt tattcatccc
    13801 tgcccattga aagggagtgt gggcttttgg cacaaagggg ttgatggata gagttattgt
    13861 tcttttcgca gaggaatcct taaaatagat ttgatgtatg tttttcaaca attcaaccat
    13921 agcatctata tcctcatctt tttcaaatgg caagccaagc atgaaataca atttcagaga
    13981 attgatattg ctctgtttag ctaattcaat cgattttaaa atctcatcca aatctatttt
    14041 cttatttatt atcttttgca ttcgggatga gccgacttca ggtgccaggg ttaaggtttt
    14101 cattccagat ccagaaagat atttcaagag attagaattc aatctatcaa ttctcatcga
    14161 agaagtcttt attcccagac ctcgctgctt gagctcttgg cagacctttt cgaaatcagg
    14221 atagtcagat accaaagtcc cgactaaacc gacctgccta tttttcccga tataattctc
    14281 tatctgcctt aatatatttt ccttgggcac gtatctggtg ggctggtagg cgaaaccagc
    14341 tgcgcaaaat ctacaacctc ttttgcatcc tcttcccacc tcaatcagaa aggtatcctt
    14401 aaaatgaatg taaggagaag tcaccggcga gaaggtataa tacttgtcca aatcaacttt
    14461 cctgggttta attggcagag gaacatcatt tttcttttca atccttttca gatatccttt
    14521 gctgtcgcat tcgacttcat aaaacctagg cacgtaaacc ccttgaatcc gagctaattt
    14581 agccagaagc tcgtcttttt tagatttctt aaatgaatat tccaagtagg ttttcatgaa
    14641 ctccattatc atctcctcag cttctccaac taagatgcag tcgaagaacg gtgccattac
    14701 ctccggattc aaactgggag ctattccccc ggcaacaact aagggatgat tctcttctct
    14761 tttagaatgt gtcaagggta tgccggcttg gcttagaatt ttcaagacat tgggatagtc
    14821 catttcaaat gacaaggaga aagatatcaa agagaaagtg ttcagttttc ttttttcctc
    14881 caaactttcg attcccaagt ccaagaaaaa tctctgacaa ctgatattgg gaatctggtt
    14941 tagtagtttg tagatagtct ggaagcccag gttagccatg ccgacggagt agtcattggg
    15001 aaaaatcaaa gccactttgg tgggagcatt tcttttaaga attgctccct tctcagtgga
    15061 aattatcttt cgcctcaagc tctcttgcat aaaataaatt taaccggcaa tttaggtttg
    15121 tcaaaacaaa atggagaaac cgccgagaga atattctggc aaattctcca ttttcttatc
    15181 tttaaagtga atcgttattc agtctgtttc ctcaaaaagg ccgggacttc caggtcatct
    15241 gtggcaaacg atttcacccg atttttctgg atcacgacct gggatttggc atctttgttt
    15301 aagctattta ctctttcagt cagggacttg cggtgaccga acaaatctat gatatcctca
    15361 tcttgcttta atccctttct caaaggcata tccattccaa agccggtggc gatgaccgta
    15421 attttcatct catccttgag atttgaatct attactgctc cgaagatgat gttggcatca
    15481 gctccagcat tttcaaagat tatggaggcg gcctcgttca cttcaaacaa agtcatattt
    15541 tttcctccag tgatattgac cactactccc cttgccccaa caattgagat ctcatccaga
    15601 agagggctgg aaattgcact gtgggctgct tcagaggcac ggttttcccc tttgccgtaa
    15661 ccggttccca tcaacgcatc tcccatttcc gacataactg ttttgacatc agcgaaatcc
    15721 agattgatca gccccggcac agtgatcaag tcggatatcc cgcgggtggc ttgcaagagg
    15781 acgtcatctg ccatcttgaa ggcgtcagtc aatttagttt ccttgttgac aatcgccaga
    15841 agcctctggt taggtatgga tatcaaggta tcaactcttt ccttgagctg gtctatgccg
    15901 tttatggctc gagccattct ctttttaccc tcaaaggtga agggtttggt taccactgcc
    15961 acagtcaaag caccctgctc cttggcgatc tcagctataa tgggggcagc accagttccg
    16021 gtgccaccgc ccatgccagc agtgacgaag accatatccg cgcccttaag ggcattctcc
    16081 accagttcac ggtcttcctc aattgctctt cggccaatct ccggatttgc acctgcaccc
    16141 aagcctcggg tgatgcgcga gccaatttgt acccggttgg ataccttaga agtttctagg
    16201 gcttgagcgt cagtattgac cgagatgaat tccaccccgg ctagactagc atccaccata
    16261 tgattgagtg cattcccgcc tgccccgcct acccccacta ccttgagttt agcataattc
    16321 tctatctcgg catccaattc aaaagccata cccaacctcc ttttgaaatt ttagaaatat
    16381 tctttgaacc aatttttcgt tttatggaac gctttgccaa atatgttctt ggcagcggtg
    16441 cttcgatatt tccccttgcg aggacgttga aatccgtaga tcaccagccc cactccagtg
    16501 gcgtggatgg gactgctggc gatatccacc aagccgctta tctgtcgcgg tgtgccttgc
    16561 ttgaccggca tatcaaaaat ctgttcagcc aattcgatgg caccttccat aagtgagcca
    16621 ccaccagtta agactactcc cgcagccagc atatctgcca aggtcgtcct ttttttttcc
    16681 ctttgagcca gggagaatat ctcctccata cgcggctcaa tgaccgagga caggacgttt
    16741 ctggaaacct ctctgggctc tcttcctcca acgcccggca cttttattat ctcttccggg
    16801 tcaaccaaac tggataaggc acaaccataa gttaccttaa gctcctccgc cttatccact
    16861 ggcgttctga gtccgatagc gatatcgttg gtgacattct ttccccctaa cccgatgacc
    16921 gcggtatgac gtatgcttcc gtcatagaat acagccatat ccgttgtgcc gccacccaag
    16981 tctatcagca ccacacccaa ttccaactcg tccggattaa gcagggcata actcgaagcc
    17041 agtggttcca aaaccagatc caagacctcc aaacccgccc tcttaattgc tttatatatg
    17101 ttttgagcag aagttactgc tccagtgaca atatgcacct ctgcttccaa tctaacccct
    17161 gacatcccca ccggatcctt aatcccagac tggtcatcca cagtgaattc ctggggaaga
    17221 acgtgaatta tctctcgatc catagggata gccactgcct tggccgcatt tatgactctt
    17281 tccacatctg ctggaccaat ctcattgtcc gacctagaag tggcaatcac ccctcgactg
    17341 ttgatgctgc gaatgtggtc tccagcgatt ccggcataaa ccgcattgac ttctattcca
    17401 gccataagct ctgcatcctc aactgccttg actattgact gaacggtttt ctctaagtcg
    17461 atcaccactc ctcttctcag tccctcagac ggactaactc ccactccaat tatctttggc
    17521 tctccttgtt cacctaactc ggcgattata gctgcgatct tggttgtgcc gatgtctaag
    17581 cccacgacta aattgttgtt tcccatctct attcgaatct cctcactatg gcttgccctt
    17641 tgaagcgcaa gtcaacatac tctatttttt caagctcttt ttcttccttt aatatcaaat
    17701 ccaaacgaat aagtttcttt tcaaactccc cacatcccaa gaatatctta gctcctctgg
    17761 gaataaggta aagaatcaca ttatcctttt cctcaaggtt gatctcggaa acaaggttca
    17821 agatgttggc atctatttta gcaagtgaat tatgtaaaaa caagacaaaa tcaatttttc
    17881 tgtctcttat cttatggtaa aaagttgctt ttttgaggtt aatgccgttc actaccggta
    17941 aatcaagatt ctcctccaaa ggaattattt ctccattttg ggttaggcca tacatatccc
    18001 ccagattgat aagatagatt ggttttttct cgctcaaatt tatcaatatc ttctggggaa
    18061 atcgtctctc tatctttaca gtttctattc tcggctcaga tagtatcttt ttcctgattc
    18121 catccatgtc tatctcgaaa aagttttgcc ccagcttaac cgggcacagg ctcaaaatat
    18181 ctgaataact taccctttga ttcccttcta tctggatggc ttttattttg aagccaggaa
    18241 agttggtaag aaaaatgaat gttcctatca gggctagggt caaaagaaaa gctacgagaa
    18301 gatttcttat gataagcata acttattttt gcaaaagatt tagaagttgc tccgccttct
    18361 ggtatatgtc cccagctccc atagttatca gcatatcgcc gggcttgagc gtatccaaaa
    18421 gaaaagggag tatctcctcg cttttgggtt tgtagtgaac ctgcttatgc ccgtatcttt
    18481 tagctgcctg gctcaccagc tcgccactca cccccggcat aggctcctcc ctggctgcat
    18541 agatatccgt gaccactaag atatcgcaat tgaagaacgc ttttccgaac tgttcggcga
    18601 agtcttttgt acggctgtaa aggtgaggtt gaaacactac cactatcctt ctgttccagc
    18661 catttttggc agcttttaaa gtggcctcta tttcagtggg atgatgagca tagtcatcta
    18721 caatcattat tcccttggac tctcctttta tttcaaatct cctctttacc cctctaaatt
    18781 gtctcaaagc cagtgtgatc ttctcccagg ggatatccaa ctccaaacca accgaagccg
    18841 cggctaatga attcttagcg ttgtgcatgc ccggaactgc caattcgata tttcccagct
    18901 cttccccttt atatttcacc gtaaatctcg accggttttc cgatagtttg atgttttctg
    18961 cttgcatatc ggcctgtggc ataagtccat aggtggttat aggcccttta agtttgggta
    19021 tcaaattctg caggctctcc tcatccaaac acagaattaa tcttccatag aaaggaactt
    19081 tgttagcgaa ctcgacaaag gcggatttca gctggtctaa ggtttgataa caatccaagt
    19141 gctctgcctc gagggtagtg atgacggcaa tcatagggga gagtcttaag aacgatcggt
    19201 cgaattcatc tgcctccgca actagatatt cgccctgccc cagcttagca ctgctatcca
    19261 gatttaccac ctttccgccc acgattatag tcgggtccaa acccccttca gttagtatct
    19321 gtcctaccat agaggtggtg gtggttttgc cgtgagttcc agcaatagct atcccatatt
    19381 tcatccgcat aagctcgccc agcatctccg cccttcttat gactggaata ctcttctcca
    19441 gagcataagt tacttcaaca ttgctcggct tgacagctga agatatgacc accacatcag
    19501 ctccttcaat attttccttg gaatgtcctc caaagacctt gattcccaaa ctcttcagct
    19561 tttcagttac ttcgcccaaa ctgatgtccg agccggtgac ttggtatcct gagttgaaca
    19621 aaacctcagc taagccgcac attcctgccc cgcctattcc cacaaaatgt atttgcttta
    19681 cttttttaaa cttcatcttt tcaatagctc ttctatttca tccgttatct tggaagtggc
    19741 atccagctta gccatgttcc ggctggcttg cgacatttgc tccaacacac ttttatctgc
    19801 aaaaagtcga tttgataagt tagccagttt ctttccattc aaatcatttt ccaagatcag
    19861 tttggcagca ccgctttttt ctaattcctg tgcattaagt ttctggtggt cagctgtggc
    19921 agaagggtag ggaatcagaa tggctggctt accgcaagca gttatctccg ccagagtcaa
    19981 tgctccagcc cttgatataa ctaaatccgc aaccgcataa gccgagccca tatcatctat
    20041 aaatggatga agacttaaat tcattttgat ttctcgtgtt ttctcttttg tggcatcgaa
    20101 atctttttct ccagtttgcc acaagagctg aaggttgtct ccaaaataat ccagagcttc
    20161 caaaactgtt aaatttatct tgtgtgagcc ctgacttcca ccaaaaataa agattgtctt
    20221 tttatccgga cttagattga atctcttcag tcccaggtct cggttacctt gagttacctc
    20281 ctttctaatt ggatttccca atatcttaaa tttcttcggt ttactcgtaa agctgggaaa
    20341 atattttagt gaaccgggat aggtcaagca tactttatcc actcttggag ccaaaaggcg
    20401 agtggtcatc ccgggaaaac tattctgctc ctgaatgagg gttagtatct ttttcagact
    20461 ggccagaagc agtaccggat agctaacata tcctccagtt ccaattacta ccccgggctt
    20521 aatcctgttc aagagcataa aagattgagc cagccccttg aacagataaa agggagcaga
    20581 gatgttcctt agtatgtttt ttcggctcaa tcccacaatc gatattgtct ccagattaaa
    20641 tccgagttta gaaagctctc tggattccac acctcttttt gttcccacaa agaataactc
    20701 aatatcttta tatctttttc ttaattcctg agccaaagct atacccggga aaagatgacc
    20761 tccggtccct cctgcaacta tgattatcct caccataaac ttgattttcc gaccacttta
    20821 gattctttgc aattgtgtct ttggcgcgag atgttcaaaa gcacaccaat tgctaggaga
    20881 ttgagaaaga gggaagtgcc accgtaactc aagaaaggca gaggcaagcc agtggtgggc
    20941 aatatcccac taatgacaca agcattcata atgaaaccaa acataacagc ggaagttatg
    21001 ccgaatgcca gaaagaaccc aaagtaatct tgtgccttga aggcgatttt aaatcccctc
    21061 caacccaaaa tcaaaaagag aatcaaggta gctgccaata gagcaaatcc accttcttct
    21121 cccatattag ccagaatgaa atccgtgtga ggttccggca gaaaaaggag tttctgctta
    21181 ccttcaccca agccaactcc ccataagccc ccacttccca aactcaaaac tgattgcgtg
    21241 agttgataac ttcctgccag ggggtcttta agtgatttga aataatcatc cattctttct
    21301 ttttcatatc ccaagccaaa caccaagaaa gggacgaccg ccaacgcagg caggataacc
    21361 agggatagat gtcttaatct ggcgcctgct atgaagagca gtaaaatgcc agtcaaaccg
    21421 atagtcaaag ctgttcccaa atccggctct ttgatgatga gcaaaaatcc aactgccaag
    21481 agcggaaaat aggggataaa aagatacttg aattccttta ttttctccag tctcttggaa
    21541 agtgaataag ataggaaaag tatgaaagcg aagcgaaaaa gctccgacgg ttggaatcca
    21601 aaaggtccaa atcttatcca ccttcgcaca ttattcatcg gtggcctcaa tagaaccgct
    21661 accagaaaga aaagcgaaag cagaagcaac gggagtgata ttttttgaaa gatgtgatag
    21721 tccaggcgca tagctaaaag cataacggga aaagccataa tgaaccaaag aagttgtctc
    21781 ttaaagaaat agattgtact tttatgcctc tcgtctccaa ccatagctga agcgctatat
    21841 accataacta tccctatggc taccagtatc gccaccacca gaagaagttg ataatccggc
    21901 cttgatttgt cggtcatttc aggttatgca ccgccagttt gaattttcta cctctctctt
    21961 caaaatcctt aaacatatcg aagctggcac aagcagggga gaggagaata acatctccct
    22021 cttgtgcctt ttcgaatccc agcttcaccg cttgctctaa tgtttccgct tcaatggtat
    22081 ccaccaaacc acctaactct ctttttatct tttccttggc ttggccaatg aggattaatt
    22141 tctttacttt ggatttgacc aattccccca gtctggtgaa atctccacct ttatcttttc
    22201 ccccggctat gaggattata ggctcatcta tactctgcag ggcgtaccag acagagtcaa
    22261 cgttagtggc tttggaatca ttgatgaact ttatgccatt aattgtggca acatattcca
    22321 ggcgatgctc caccccctta aattccttca gggcgcgagc catagatgct gtatcgatct
    22381 ctaaagcata tgccatagcg caggcagccg ccgcattcga caaattatga ggacctttga
    22441 tggttatctc tttaatcgga agaattgccg tctccccttt tttgcttttc aaaaccaaat
    22501 tctcgcctct taccaagacc ccctcttcca tgtctttctt gatgctgaaa tatatttgct
    22561 tggcttgagt gcaaggagaa agagatgagc tcacgaggtc gtccgcattt aaaacggtat
    22621 agtcttgtgg gatttggttc tctagcaaac gcaccttggc ttcttgatac gatttcaaat
    22681 caggataacg gtcaaggtga tccggcgtta tatttaaaat agaagctacc tttggtctga
    22741 actcctctat tctctccagt tggaaactcg agacctcaag cacgattatc cctttatcag
    22801 agacttcatc cactacgctg gaaaacggca ctcccacatt tccagctacg cgatattcct
    22861 tctggtcctg ccttagaatt tcgcccacca gataggtggt agtggttttt ccgttggagc
    22921 cggttatagc tacaattggg ctttggctta accagaaggc tatctctatt tcagagaaga
    22981 tcggtatgcc cagactctca gctttcttca agataggtat gtccgatgga atacccgggc
    23041 tcgtgacgat aaaatcctta tcttctaaaa ctctgtcagt atgcgctcca acttcgaaat
    23101 ttatatttaa acttttgatt ttagctatct cgttctccaa ttccctttcc gacttgatgt
    23161 cgctcacaaa gacttccgca cccttctggg aaagaagttg agctgtggct attccagaac
    23221 gagccatacc taaaacagat atctttcgat caaataccct atcctccctt ttacccactt
    23281 ttctctctcc gcttagaatc tgtatcatct tattttcaat gtgcttaatg ccaaaagggc
    23341 aaatatcact cccagaatcc aaaaacgcac caccacttgt ggctctttcc agcccgaaag
    23401 ctcaaaatga tgatgcaggg gtgccatcct aaaaatcctt tttccgccag tggctctgaa
    23461 atagactacc tgaattatga ctgataaagc ttctatcacg aaaaccccac ctgcaatcaa
    23521 aagcaacatc tccttcttta tcaggataga tataaccccc aaagctccac ccagagccaa
    23581 agccccggta tctcccatga agactttggc tggataagcg ttgaaccaca gaaaacccaa
    23641 agccgcaccc atcaatgcac cacaatagac caccaattcg ccgcttccct gcaaatagac
    23701 cacggaaaga taacggctaa aatcgactct tccagcaatg taggacaaga ccgcaaaggc
    23761 aaaggcgcat atgccgacca gaccgatggc taatccgtct aagccgtcgg ctagattcac
    23821 cgcattagat gagccagtaa ttaccagtat gactaagggg atgtaaagaa ttccaaaatc
    23881 caaaaagtaa ttcttgaaga aaaggatgtc agttgaagtt ccgaaattct tggagggtgg
    23941 ttccaaaaga agccacaagc ccaatactaa ccccaagctg atctgtccca ttaacttctt
    24001 tcttcccacc attcccttgg gcactctttt tatggctttg agataatcat ccataaaacc
    24061 aatcagcccc atccagattg tagccaacaa aatcacctga atatagcggt tggctaaatc
    24121 agcccacagg gcagtgggca caaccacaga gagtaatata ataattcctc ccatagttgg
    24181 cgtcccctcc ttcgccatat gcgtggctgg cccatctgcc ctgatgtttt cagtaacctg
    24241 ttttgttttc aaaattctta ttatcattgg tccaaaaatc aggctcaaaa gcaaggcagt
    24301 gactgaagca taagcagttc tgaaagttat atatctgaag atattgaaaa aggagatatt
    24361 ctggcttaac ggataaagca ggtgatatag catttagttt tgcctgttta gaaaattgtt
    24421 cagttcctcg actatctcct caaatctcat tttgcgagaa gctttgaaga gaaccatatc
    24481 tccagtcttt agattatcaa gtaaccagga gcttatcttt ttgttttctt taaatatttc
    24541 tatttttgtc ttatccatcc cttttcctgt tgccccggca gcaactgatt cggaaaactc
    24601 ccccctaagt aaaagcttat ccagattcaa ctgcccaact tgttctccga tattaaaatg
    24661 aaattcatcg cttttttcgc ccagctccag catatccccc agaaccgcaa tcttcctacc
    24721 ttggcttttt atttgatcta aagtctccaa tgcattttgc gttgaaatcg gattggcatt
    24781 gtaagcatcg ttaattaagg taattcccct tactttaaac cattccattc ttagctgagc
    24841 gggcttataa ttctccagcc tttgtttgat tagatcctca tctatgccca gctctttagc
    24901 cagggcgaag gcagctaggg cattatagat attatgtctt cccagcacct tgagtcttat
    24961 ctgaggttgg gaattaactt tgaaatggta atgtccgctt ccatttgact cgatcgaagt
    25021 tgccctgaag tcagcttgtt tttctattcc gaaagttata accctctttt cttcttcttt
    25081 tatcctcctg ttcaagaaaa tgtcatctgc attcaaaacc aaagtcttgt cctttgccat
    25141 attgtctgca agctcgaact tagctttagc tatattctcc ggagatttca tgaattccag
    25201 atgagctggt ccgatattgg ttatcacccc ccaatccggc tgggtcagtt tggttaattg
    25261 ggctatctcc cccaaactgt tcatccctaa ttccaagacc aagatttcgg tttgaagttc
    25321 aatttgaaaa agagttagcg gaatcccaat aaggttgtta taactcttgg gtgaccttag
    25381 aactttatat ttttctgaaa gaacagctcc taccatctcc ttggtagtgg ttttgccgtt
    25441 agtcccggtt atccccacag tgggcagatt gaattttcta cgataccacc aagccaaatc
    25501 atataaagtc ttcttggtat cacccaccag tattatgggc aaatgctctt tatttatgtc
    25561 accgactctt tttttctccg ccacagccaa gatcgcaccc ttttcaaaag cttggggaat
    25621 aaatttatgc ccatcttgat tttcgcccct aatggcgatg aagagattac ccttctgaag
    25681 ggtgcgggag tcaatagata cccccttcat cttcttatta gaagcaattc tgtctttaag
    25741 gagctttcca ttgcttactt taagaagttc atttatagaa atttcaatca ttacctttat
    25801 accccatccc tttcagaatt tcctctactg cctctttatc actgaaatga aatcttttag
    25861 tcccgattat ctgatagtcc tcgtgtccct ttccagcgat gaccagaatg tctctgggct
    25921 tggccaaatc tattgcctgc tttattgcct cttttctatc ttctattcgt ttaatgtcat
    25981 tcttgctgtt aatccccttg aatatgtcat ctataattga actcggattt tctgtgcggg
    26041 gattatccga ggtgacgata acataatccg caatccgggt agcgagtttg cccataattg
    26101 gccttttagt cttatccctg tccccaccac atccgaaaag ggctatcaat tttccagaag
    26161 tcaattcctt ggcagtgatg agaactcttt ccagggcatc tggagtatga gcataatcta
    26221 ttaagatgtc gaacggctgt cccaagttga ttttctccaa acgaccggaa acaactctgg
    26281 ctttctctaa gccagatctt atattctcca attcgaaacc cagcgcaact gagctagcaa
    26341 ttgcagccaa tgcgttataa acgttctgct ctccgatgaa attcaatctt acttcaaatt
    26401 cacctaaggg ggtagaggat cttatttcta aaccctttaa atcatgattc attttctcta
    26461 caaatacatc cgcctccttg tttcttaaag aataagttac ctttggagct ttggtctttt
    26521 tggaaaagta tttccaattg gggtcatcca aatttatcac tgctctatcc tctggtttga
    26581 ccagcatctc aaataacttt cccttagtct ttttatatga ctccatatca tgatgaaagt
    26641 ctaaatgatc ctgagtcaaa ttggtgaaaa cagccacatc gaagtcaacc ttctccactc
    26701 ttttgagtgc taaagaatga gaggagacct ccatcaccac atattttatc ccttgctcca
    26761 gcatctggta aaacattctt tgtaggtcta aagattccgg ggtagtgttt agggctggca
    26821 cttgttggtc cccaatgtaa tgggctatgg tgccgatcag tccaattttt ctttccttgg
    26881 tttcgaggat agattttaga agataactga ccgtggtttt tccattggtg ccggttatgc
    26941 caattacctt aagctttctt gaaggtgaat cataaaattt gtctgaaagg aaagccaaaa
    27001 ccattcttga atctttcacc agaatttccg ctctaatatt gtggtcttga tctttctccc
    27061 ccacaacgca aatcgctcca ttcttaatgg catcgtttat aaaattatgt ccatccgtct
    27121 gaaatccctt aattgccaca aacaggtcac caggttttat ttttgtggaa ttatattcta
    27181 tcttgttgat atcaaaatca gtatctccat atgttttttt tgtattcata ctttcaatca
    27241 gttttcttaa aggcacaccc atatttcact ccgtttcaca atgcaaaaga caaatctgtc
    27301 caggattgag cctgcttcca acagaagggg tttgattttt gaccacgccg cttcctatta
    27361 tcttgaaatt tatcttttct tttgaaaaga gatgggctgc ctgcctgact gtcattccca
    27421 atgcattcgg aactacaatt ccatcagtgg aagcatctgc caagttctcc cccagcataa
    27481 ttggcatgcc accaacctgt ttcccttttg gtttgcctgt tgaagtttga gagataatat
    27541 tcttttcgga ggtagtggtt ttgactaaga ccaaaggctc atcctgtaaa ttagccagac
    27601 gctgcgccat atttctgaaa gcaggagccg cagtctcgct accgtaatac ttaccttggg
    27661 gattatctaa acatacaagg cccacgaact tgggctggct cgcagggaaa tagccaatga
    27721 aggatgataa atactcgccc cgtttgtatc ctccatttat tgaatccggc ttctgggcgg
    27781 ttcctgtctt tccggcaatg tctaacccct cgactttagc agggtacccg gttccagagt
    27841 caaccaccac tctaaaaagg tcattcatga tttggcaagt tcggggagag atagcgtgcc
    27901 ttatcttctg tggatggaat tcctctacgg tatcaccttc ttggttgagt atcgccttca
    27961 ctacatatgg tttcatcaag taacctccat tagcaactgc agcataagca ttgactaatt
    28021 gcaggctgtt gagtgaaacc ccctgtccaa aggcgagggt ggagagaaca aaatccgagc
    28081 actttctgag agaggggaaa gctcccttag attcactcgg aaggtcaact ccagttttga
    28141 ttccgaatcc taaatttcga gcatacctgt aaagtttgtt ctttccaact tgcagggcta
    28201 cctttgcaaa agctatgttg ctggaataag cagtgcattg cctgaaagtg agccattcat
    28261 acttgtgcac atcatgtatc ttatggttgt tcacctcgaa ctcacctttt tcagcgtaaa
    28321 tcgaatcttc cggatttttt actttttcct ctaaggcggc aaccgcagtc actatcttga
    28381 aagtggagcc aggttcgaaa agatcgctta cgcttttggt cttaagggat gggctattcc
    28441 cattcttggg aaagtgagcc atagctagga tttcgccggt tttggggtcc atccatacac
    28501 ttgtccctcc gtccgcctgg tttgactcaa ttgccttgcg caattcctcc tcaacaattg
    28561 cctgaactct tagatccaaa gttaagacta aactatttcc attttcacat ttgaccaacg
    28621 gttccgcctt taaagcgaaa ttgcgcattt tggcatctcg gttaattaat atctggccag
    28681 tctttccccg cagagtctgg tcatattgat attccacccc agctgctccc tggtcgtcga
    28741 tattagcaaa tcccaagacc tcgtccgcca aatctccaaa aggataaacc cgcttgcttt
    28801 ccttttttga atacactcca accagattcc aggagcgtat taactcgctt tcttccctat
    28861 tgacttttct tttaagccag gcgaatttct tgtagtgctt gagatcctta gttatctctt
    28921 gaggagtaaa ggaagtaata gaggagaatt tttctgccac cgaatagata tcgcttagac
    28981 tctccggaat ggcaaagaaa gattcgcttg gcagattgaa agccaaaact tggagatttc
    29041 tatcgtaaat cgcccctctt tgtggggaca cttccaggca gagctgatgc tgcctatcgg
    29101 agatcttctg gtatattttt ccctgaacaa cctgaatttg aaagagccgc aggattagaa
    29161 taaaccaacc taaaagcccc agcatgagaa taagatagaa tcttccctgg ggaactttat
    29221 aggtcatgct ttacctcacc agcctccact tgattctctg agatattggt catcttctca
    29281 actgcttgct tcgctttgga taatagactg gttggtttct cagaagtttt gggttcaacc
    29341 aaaaaagata tcggttggtc ataagttgcc atgccaagct cgctttgggc tattctctct
    29401 atattctgag ctgagcctaa cttagtcaca tctaggctca ggtatttgta ttccgcctta
    29461 caactggata gttggttttt caaagtttgt atttcctggg cagattctat cacacatacc
    29521 ctttgccaca cataaactaa agccaaaccc acaactaata agaggattag aacgattctg
    29581 aaataaggga agctggtatc gcttgaaatt tttcttttga atttctttct catggtatcg
    29641 atttatccga gtttagctgc tactctcagc ttagcgcttc tgcttttggg atttgccttt
    29701 atctcctcaa gagatggaac tatcggtttc ttagtcagga ctcgaagaac ttgcttttgc
    29761 ccgcacctgc aaattggaaa atagggaggg caattgcagg tttgggacaa ccgacgaaat
    29821 tggttcttca ctatcctatc ttccaaggaa tgataagtta ttacacaaac tctaccctct
    29881 ggcttcaaaa aatcaactgc ggtctttagt cctcgtttca gctcttccaa ttcctgattt
    29941 atctcaatgc gcaaagcctg gaagatacgg gctaaactct tgattttatg ttttggattg
    30001 actttgtttt ctattatttc tcttaagtgg ccagtggtcc taatttccct cttttttctt
    30061 gcccggacta tagcagaagc aattcgcctt gagaatctct cctcgcccaa ttcaaagaaa
    30121 atcctggcta gctctttggg gtcataagag ttgaccacgt ctgcggcggt tttactctgg
    30181 gcttgatcca tacgcatatc cagaggacca tctgacaaat aggaaaagcc ccgatgtaca
    30241 ttctctatct gggctgagct tatgcctaaa tcaaagagaa caccgctaag attatctatt
    30301 ttcaattcct tcaaaaaatc ttctaattga ctgaaataca gcttttgaaa aacaacctgt
    30361 tttccaaatt cagctaaatt gctcttcgct ttacgtatgg cttgttcgtc gcggtcagtg
    30421 cagattaact ttcccctctg gtcaagtccg gagagtatgg cataagcgtg tcccccttct
    30481 cccacagttg catccacgta aataccagat ctatcgacaa tcaaatactc ggtaacttca
    30541 gataaaagta ccggtatgtg aaccattacg aaatgacctt taaaggaggg ttttctctgc
    30601 tacctcctca taagattgtt caaagccatc caagtacttc tgataaacct ccggagacca
    30661 gatctcaatt cgctcaagca ctcccaagac caagacctct ttttttatct gggagagctc
    30721 catcaagggt tgagggatgg ctatccttcc ctgcttatca acttgaacgt cactggcatt
    30781 ggacagaagt aatcttgtga aatatcgatt gtggctttga gtgaatggca gagatcttaa
    30841 tttctcatct attctttgcc actcctcagt gggataaagg aataaacatc cttccagccc
    30901 acgggtgatg acgaagctct catgcaaagt ggaagtgccg gttttacgga actttgctgg
    30961 tatatttatg cgacctttat ggtcgacagt atgacgatag aaacctttaa aagtagccat
    31021 aaagtaagcg atttaccaca tttccccaca ataacccaca agcgaacaca tattactcca
    31081 attcaccatt tttgtcaaga gaaaaatcac agatttcaaa acaaaatttt cttcgtaaga
    31141 tgttggaaat aaaccacaat atgtgggttg attcccacta tattttgtag gaaatcgatt
    31201 gtcgacgtta ccccatccat tcacttctgc cgaatacccg actccaagtt aatatagaga
    31261 ctaacgttat ctcaattgag ttcgattcgc ccagaatcgt tttctttggg cacagttatt
    31321 gcctaactgg ttacaagata tgcagctata gaaatcccta ttgcggctta aggagggggt
    31381 aatgaggttt ttaaggtttt tcttttttat tttttttgtt cctgcgttat gcgtggcaga
    31441 tatcactaag gaaatccatt tcgatattgg ggatttgacc cggtctgaac aagatggtaa
    31501 aatttatata aatctaaaag atagccagag agaatttaga gttggatatc cggatttacc
    31561 gataaaactt ttgactttca tcattccttc agatgttgaa gtggattcac ttgaggtcag
    31621 ttctttgagt tcggatacat tagaagaaga atacaccatc tctttggttg gagctgacac
    31681 caaaacagat gacaattcgg caattgaata ccaagagaac gatacattac tatacaatcc
    31741 agatcttccc tatccccaaa agttggcatc tattctcaat tgcggatatc taagtggaaa
    31801 tcacctcatc actttgacag tttatcctct gcaatatttc gccgcgggaa aaagattggt
    31861 ttttcataat gatctaagaa taaagctgat attcaaaccg aatcaaaata attcaaattc
    31921 attcctgatg aacaagaaag taaatcaggg tatcttagag gaactggtct ttaataaaga
    31981 agatatcctg tcttgttcgc cagctgtgtc tctatcaaaa cagatatcca tacctgcttc
    32041 agcccaatac atagtcataa ctaaccagga gatgaaggat gccctcgtcc ctttaattga
    32101 atggaaaaca aaaaagggca ttaagactga aatagtcaca gttgacagca tatacagcta
    32161 ttatgcgggt atggattttg cagaaaagat acgcaacttt ctaatagagg cttatcaaag
    32221 ggggttagaa tgggttcttc tggccgggaa tgaggatatt gttccggtca gatacgctta
    32281 tcagagcaat gcttcgaccc ctccttcaat tgctaaccag caaatctgcg acctgtatta
    32341 ttccgactta actggaaatt gggaccagga tggagatgga atctggggtg agcccctgga
    32401 tgacagcccg gatatttacc cggagatatt cgtgggtaga gttccctgcc agaatgctca
    32461 ggaggcagga aattttgtag aaaaagtttt gaattatgag agaaatccga ttgagggggg
    32521 ctcggattat ctcaatcgag ctttatggat ttgctccgac cagatgagag attggaatga
    32581 ggaagagggg caacacaaac ttatctctcg atacattccg accaatttct atcaggagtt
    32641 aaataattta atagaatccc cttccggaga cgcacctgac ccggttagcc caaagggcca
    32701 aaactgcata gagtcaatga accagggttg gggaataatc ggcatacttg ctcatggcag
    32761 aatagatgga tttgtagcct cttctaacct gataaacgaa tggcctaaat cttacgtgtt
    32821 tagctggcca gcagaagaag atgggcatgg ccatcttact tcgttgtcca accaggggaa
    32881 atatggaata gtctattcca tctcctgcaa tcaaggcgcc atagattatg ataaagagtt
    32941 aacgctgggg ggtggcacga gtgtggccga gtcttttatc ttatcttgcc agaaaggtgc
    33001 agtcgccttt ttaggatatt cccgctgggg atgggtttca tcctcttata agttaggtca
    33061 agcttttatg caggagctgt tcgactccac aaatgactat catctgggca tggctgaagc
    33121 tttgtccaaa ttatattatc cctactactg ggatttgaat tatggccaca atctttttgg
    33181 tgatccggaa atgcaggttt ggacccaagt tcccggcagt ttcagtgtga gctatacaca
    33241 aacaatagaa actggaagcg aggatgcaga gtttcaagta agcacaaatg gaaatccagt
    33301 tccccaagcc aagatatgca taaccgaagg aaatgaaatt ttgtttttgg cggaaactga
    33361 tgaacagggg aagttgagtg cacaattaga tatcgatcat tgcactgaat tagcagttac
    33421 cattacgaaa actaacttca ctccttatca gggaacaatc aaggtttcgt ccactgctgg
    33481 tgtaggtgaa aatcaagacc cggtacccct aagctttgaa ttatcccaga actatcccaa
    33541 tcccttcaat cccactaccc agataaatta tcagatccct gaagatg
//
LOCUS       DASWLN010000186        13840 bp    DNA     linear   ENV 20-FEB-2024
DEFINITION  MAG TPA_asm: Candidatus Zixiibacteriota bacterium isolate
            SMAG_U2949 k141_250274, whole genome shotgun sequence.
ACCESSION   DASWLN010000186 DASWLN010000000
VERSION     DASWLN010000186.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37960594
            Sequence Read Archive: SRR10376091
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Candidatus Zixiibacteriota bacterium (soil metagenome)
  ORGANISM  Candidatus Zixiibacteriota bacterium
            Bacteria.
REFERENCE   1  (bases 1 to 13840)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 13840)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (26-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/12/2024 08:28:26
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 1,440
            CDSs (total)                      :: 1,416
            Genes (coding)                    :: 1,412
            CDSs (with protein)               :: 1,412
            Genes (RNA)                       :: 24
            rRNAs                             :: 1 (16S)
            partial rRNAs                     :: 1 (16S)
            tRNAs                             :: 21
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 4
            CDSs (without protein)            :: 4
            Pseudo Genes (ambiguous residues) :: 0 of 4
            Pseudo Genes (frameshifted)       :: 0 of 4
            Pseudo Genes (incomplete)         :: 3 of 4
            Pseudo Genes (internal stop)      :: 1 of 4
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..13840
                     /organism="Candidatus Zixiibacteriota bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_250274"
                     /isolate="SMAG_U2949"
                     /isolation_source="soil"
                     /db_xref="taxon:2053527"
                     /environmental_sample
                     /country="USA: Oregon"
                     /lat_lon="42.99 N 123.63 W"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            complement(<1..619)
                     /locus_tag="VGB16_06850"
     CDS             complement(<1..619)
                     /locus_tag="VGB16_06850"
                     /inference="COORDINATES: protein motif:HMM:NF014269.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="LON peptidase substrate-binding
                     domain-containing protein"
                     /protein_id="HEX9917441.1"
                     /translation="MEIKQPQEETKKIQEELSILPLKGVVVYPFLIMPLLISSQKYAH
                     MIDDTLMHGKVIGLVTQKNPEQEEPGPDDIYKMGTAATILKMLRFPDGSVRFLVQGLS
                     RIRLKSIVKTDPYITAQVEVIEDQIRKTVELEALVRSILDQLRKVVNLAPYLPDDLQI
                     SAINTEDPGKLADLIASNLNITIPQKQELLETIDVTERLHKLTVYL"
     gene            complement(649..1095)
                     /locus_tag="VGB16_06855"
     CDS             complement(649..1095)
                     /locus_tag="VGB16_06855"
                     /inference="COORDINATES: protein motif:HMM:NF012241.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="Hsp20/alpha crystallin family protein"
                     /protein_id="HEX9917442.1"
                     /translation="MADFKKHIIRDIKKIQEEMDILFDHFYKVRHSPVLTSRRLWRPP
                     ADVIETTSEIIILVEIGGMKQKDLSISLADNVLVIRGERKERDVKNMSGYRSMEINYG
                     MFERNIYLPETVDTEEIVASYKDGLLEIRIDKKKDERMKGKQIKVE"
     gene            complement(1300..2385)
                     /gene="ychF"
                     /locus_tag="VGB16_06860"
     CDS             complement(1300..2385)
                     /gene="ychF"
                     /locus_tag="VGB16_06860"
                     /inference="COORDINATES: protein motif:HMM:TIGR00092.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005525 - GTP binding [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="redox-regulated ATPase YchF"
                     /protein_id="HEX9917443.1"
                     /translation="MKVGIVGLPLVGKTTIFNALTGGKAKIGDFALGPKNPNIGVVKV
                     PDERLYKIAQLYQPKKVTPTEVEYIDVGGLIEEEKKKGSEGQFYASLRETDQLAQVVR
                     EFENENVVHIHGQIDPKRDIKDFDCDLVLLDLDLVEKRISRLEKNLKVGRKEDKEEFE
                     LLARFKESLEGGIPLRKLKLTQVEEKLTRGYGFLSLKPMLVLLNIGENQIGDSARIER
                     EMRSCIEDEKVDVCLVCGKLEMELSQLEEKDKESLRGELGIKEKALDKMTHNSYKLLD
                     LVSFFTANQNEVKAWPIKSGSTALKAAGAVHSDMEKGFIKAEIISYGKLLEFGSTARA
                     REKGELKLEGKDYIVQDGDVILFRFNL"
     gene            complement(2630..3043)
                     /locus_tag="VGB16_06865"
     CDS             complement(2630..3043)
                     /locus_tag="VGB16_06865"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_013136842.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="thiamine-phosphate pyrophosphorylase"
                     /protein_id="HEX9917444.1"
                     /translation="MMDKGIYRLIDANLNRSREGLRVVEEVARLYLNDENLSAKMKVI
                     RHQISKIAIRFLDQDQLIKSRDSQKDVGAKGMRGLEKKREGLKGVVQANLIRAQEGLR
                     VLEELGKLVNSKAGEEFKKLRFKVYSLEKKILIKL"
     gene            complement(3171..4262)
                     /gene="gcvT"
                     /locus_tag="VGB16_06870"
     CDS             complement(3171..4262)
                     /gene="gcvT"
                     /locus_tag="VGB16_06870"
                     /EC_number="2.1.2.10"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_020251039.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0005737 - cytoplasm [Evidence IEA];
                     GO_function: GO:0004047 - aminomethyltransferase activity
                     [Evidence IEA];
                     GO_process: GO:0019464 - glycine decarboxylation via
                     glycine cleavage system [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="glycine cleavage system aminomethyltransferase
                     GcvT"
                     /protein_id="HEX9917445.1"
                     /translation="MPKKTPFYELHLKHNAKMVEFAGYLMPIQYQGITHEHKRVRKSV
                     GIFDITHMGEFEVRGKDALAFLQKVTTNDVSKLETYQVQYSCMCYAEGGIVDDLLVYK
                     LPGYYMLVVNASNIDKDFKWLQSNLFGDVKLINKSDETALLAIQGPDAEKVLSNMTNV
                     DLSKWKYYWATFGKVSGVDMLFSRTGYTGEDGFELYFPFCHAENLWNASMQAGKEFEI
                     EPIGLGARDSLRLEMKYMLYGNDIDKTTNPFEAGLSWIVRPEKGDFIGKESFLKMKEE
                     GSKRKLVPFELLEKGFPRQHYSILKDTSKIGEVTSGVFSPSLDKGIGMGYVETGYSTL
                     GANFDIEIRGKGYKAEVVKSPFYKEFTHK"
     gene            complement(4277..5557)
                     /locus_tag="VGB16_06875"
     CDS             complement(4277..5557)
                     /locus_tag="VGB16_06875"
                     /inference="COORDINATES: protein motif:HMM:NF037202.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="type ISP restriction/modification enzyme"
                     /protein_id="HEX9917446.1"
                     /translation="MSNSNKCKVFHSEVWGLRETKYDWLVKNDIKTTKWKKLNPASPY
                     YFFVPREEKLLEDYEKHPKISELFPLNSVGLFTARDGFAIDMNKDNLLNRIRLFKNNK
                     YSDDELHAFFQINRSKGWDIRKAWKAIHSISDSDLSRFILSILYRPFDIRWIFYHDAV
                     VERSRKEVMRHMIQENLALCVGRAGQVVGLEKRWNIVFCSEWFGDLNLFYRGGNVNFP
                     LYLYPDTDKKDLFSQKKKSEKRQPNINPKVFATLTEVYRKEPTPEEVFYYIYAVLYSN
                     IYRTKYSEFLKIDFPRIPFTKDYKLFSKMAEYGKELVDLHLVGAIHVGAIHESPLPKF
                     QGKGDNSVEKIKFEKGKVYINKDQYFESIGREIWEYQIGGYQVLSKWLKDRKKRKLSL
                     EDIKHYCKVVTVLDRTIQIQKIIDELYPQIEKSS"
     gene            complement(5547..6134)
                     /locus_tag="VGB16_06880"
     CDS             complement(5547..6134)
                     /locus_tag="VGB16_06880"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011937757.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003677 - DNA binding [Evidence IEA];
                     GO_function: GO:0004803 - transposase activity [Evidence
                     IEA];
                     GO_process: GO:0006313 - DNA transposition [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="transposase"
                     /protein_id="HEX9917447.1"
                     /translation="MTNNPEKYHRHSIRLKSYDYSQEGAYFVTICTQDKKYPLGKVVG
                     GNMELSSIGKIAQEYLFDISKHFANAKLDQFVIMPNHIHTIIMVEKVGAIHELPLRKP
                     PLRIRRRKMLIPKIIGYFKMNCAKKINQMQNSCGMPVWQRNYYEHVIRSEKELGNIRE
                     YITNNPLKWELDRENPFSANYNLEHKKYFEGIYEQ"
     gene            complement(6131..6892)
                     /locus_tag="VGB16_06885"
     CDS             complement(6131..6892)
                     /locus_tag="VGB16_06885"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEX9917448.1"
                     /translation="MDKSSFSKEFPQTYEFFKDLKCSAWNEIIEHPDFCPSPKLEDYF
                     TFVNQKIDKQSGKILGKKFWDASSIEQFLDYLAELQIAHFLFKNIHPQPIIEFIKEGK
                     DGTPDIRIRIGPKNVSICVEVTRMRSKGSPIEQLMEGKLLQDQGEIVGVANIENVTEF
                     ADKSFAGLLNKLIGKKSQLSNCKADYKIFAVDSGRWSIDSSYFEDTDRYPNLKSEFQS
                     NVDGILFFGCKGEKKLFSFKSNLKGLIDNVEYYEK"
     gene            complement(6895..8787)
                     /locus_tag="VGB16_06890"
     CDS             complement(6895..8787)
                     /locus_tag="VGB16_06890"
                     /inference="COORDINATES: protein motif:HMM:NF014442.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003677 - DNA binding [Evidence IEA];
                     GO_function: GO:0008170 - N-methyltransferase activity
                     [Evidence IEA];
                     GO_process: GO:0006306 - DNA methylation [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="N-6 DNA methylase"
                     /protein_id="HEX9917449.1"
                     /translation="MLQKYIKEIFEIANRGDAREESYYSILKELLNEYAESIGRKNIY
                     ITPQPKRTEAGNPDFRVWDGKQHIVGYIEAKAPTTADLDQIEDSEQLKRYLHTFPNLI
                     LTNFCEFRFYRNGSLMDKVLIARPFVIHKLKTIPIAERESEFFDLLEKFFSFSLPKVY
                     DAKDLAIELAKRTRFLKDEVIAQELKEEEKNGKGFILGFYEAFRQYLIAGLTKEEFAD
                     LYSQTITYGLFAARTRSENGFNRKLAYDNIPRTIGILRDVFKFISLEDLPQQMEWIID
                     DISEILANTDVKKILQQYFHEGKGKDPIVHFYETFLTEYDPKTREKRGVYYTPEPVVS
                     YIVRSLHNILKEHFNRKDGFAGESVTVLDPAAGTLTFLAEASKIAVDEFVGKYGKGGK
                     ENLIREHILKDFYAFELMMAPYAVGHLKMSFLLEELGYKLQKDDRFKLYLTNTLEMEE
                     LTQTELPGMASLSEESHLAGKVKKETPILVILGNPPYSGHSANVSEKYIVEMKNGKER
                     KRKTKTWIGNLIEDYKFVDGKPLGEKNPKWLQDDYVKFIRFAQWKIDQAGEGVLGFIT
                     NHSYLDNPTFRGMRQSMMNSFNEIYILDLHGNSLKKEKCPDGSKDENVFDIQQGVAIV
                     FLIKRK"
     gene            complement(8768..9388)
                     /locus_tag="VGB16_06895"
     CDS             complement(8768..9388)
                     /locus_tag="VGB16_06895"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_013262351.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003677 - DNA binding [Evidence IEA];
                     GO_function: GO:0004803 - transposase activity [Evidence
                     IEA];
                     GO_process: GO:0006313 - DNA transposition [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="transposase"
                     /protein_id="HEX9917450.1"
                     /translation="MKLEIVKISPKRRGRGSIRLKSYDYSQAGGYFVTICTHQRECLL
                     GEIMDGNVELSKIGKIAQECWFEIPKHFGDVQLDEFVVMPNHVYGIIIINQRISVGTD
                     YNLSLRRQNRFRNVIVKSLSYIVAGFKSGVTRKINRSRNISHPLAWQSRFYDHIIRNE
                     AELNRIREYIITNPLKWELDRENPFSTNYNLELKRYFDGIYVTKIH"
     gene            complement(9399..12221)
                     /gene="uvrA"
                     /locus_tag="VGB16_06900"
     CDS             complement(9399..12221)
                     /gene="uvrA"
                     /locus_tag="VGB16_06900"
                     /EC_number="3.1.25.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011339738.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003677 - DNA binding [Evidence IEA];
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_function: GO:0016887 - ATP hydrolysis activity
                     [Evidence IEA];
                     GO_process: GO:0006289 - nucleotide-excision repair
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="excinuclease ABC subunit UvrA"
                     /protein_id="HEX9917451.1"
                     /translation="MSQQIIKIRGAREHNLKNIDLDIPRDKLVVITGISGSGKSSLAF
                     DTIYAEGQRRYVESLSAYARQFLGMMEKPDVDFIDGLSPAISIEQRSSGRNPRSTVGT
                     VTEIYDYLRLLFARVGVPYCYKCGRKISQQTVQQITDTVLSLPPGSKIQILSPLVRGR
                     KGEYKELFEEIKKEGFVRVRVDAKILDVDSPIALDKNKKHNLEVVVDRLVVEKKIIKR
                     LADSLEIGLKMGAGTVLVSVGGKEELVFSEKYACAHCGTSFEELTPRMFSFNSPFGAC
                     PGCDGLGTTMNVDPDLVIPDKNLSISQGAIACWGIPVGNYYYHLLKSVAEHYGFNFYT
                     PFGQLNSKAQQVILFGSGREEIRFYWESMERKGKGEYFDSFEGVIPNLERRYRQTQSE
                     GVREWIERFMRVRPCQECNGARLKKESLSVKVGGLNINQLSQLSIKQAKDFFANLSFS
                     ERETQIAKQILKEIKERLGFLVDVGLDYLTLDRRAGTLAGGEAQRIRLATQIGSRLVG
                     VLYILDEPSIGLHQRDNKRLLNTLLGLRDLGNTVLVVEHDRETIQTADYVIDLGPGAG
                     INGGEVVAVGAPKDIKRNSKSLTGRYLSGKLSIPIPDDRRRGNGKFLSLIGACGNNLK
                     HTRVNIPLGIFVCITGVSGSGKSSLVGETLYRALASHFYNSREAPLPYSEIKGLESID
                     KVIDIDQSPIGRTPRSNPATYTGLFTYIRDLFAQIPEAKVRGYKVGRFSFNVRGGRCE
                     ACEGDGMIKIEMHFLPDVYIPCEVCKGKRYNRETLEIRYKGKNIAEVLDMTVSEALSF
                     FRNIPKIRRKLETLADVGLGYIHLGQAATTLSGGEAQRVKLSTELSKVSTGRTFYILD
                     EPTTGLHFDDIKMLLGVLNRLVDKGNTVLVIEHNLDVIKTADHIIDLGPEGGDEGGEV
                     VAIGTPEEVAKNKNSYTGQFLKKLLN"
     gene            12372..13064
                     /locus_tag="VGB16_06905"
     CDS             12372..13064
                     /locus_tag="VGB16_06905"
                     /inference="COORDINATES: protein motif:HMM:TIGR00044.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0030170 - pyridoxal phosphate binding
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="YggS family pyridoxal phosphate-dependent
                     enzyme"
                     /protein_id="HEX9917452.1"
                     /translation="MGSIRENLKIVRERIDQAASKAGSRGDDIKLVAVTKNVDVERIK
                     QIIACGIEIIGENRVQEALSKFDYLPAEVEKHLVGHLQTNKAKKAVRLFDMVQSVDSS
                     DLAFEISKRANEINKEVPILIEVNTSGEESKFGIEPEEALPLIEKIGALDNIRIGGLM
                     TVGLWSGEESKVRPCFVKLRKLFERMKEQNIPDVQMKYLSMGMTSDFEWAILEGSNMV
                     RIGTGIFGPRVV"
     gene            13117..13683
                     /locus_tag="VGB16_06910"
     CDS             13117..13683
                     /locus_tag="VGB16_06910"
                     /inference="COORDINATES: protein motif:HMM:NF016959.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DivIVA domain-containing protein"
                     /protein_id="HEX9917453.1"
                     /translation="MKITPLEIKSQEFGKSFRGYHTFEVDSFLDNLAVEMEELVRENN
                     LLKEKLKGLEEKLGEYKKIENSFQEMLLITQKSTEEIKKNAEKEADLVVREAEVKAGK
                     ILEETFGRLSELKNQIANLDSLKRSYVLKLKSALKAQQQLLEEIEKEDISTTKLNFRR
                     RADLTEEELNRVVEEFKKEDDLSPEETN"
BASE COUNT         3796 a         3156 c         2501 g         4387 t
ORIGIN      
        1 taagataaac ggtcaactta tgcagtcttt cggtcacatc aatggtttcc aaaagctcct
       61 gcttttgagg aatagttata ttgagattgg aggcgatcag gtcagctaac ttgcccggat
      121 cctcggtatt gatagcggat atctgcaggt catctggaag atatggtgct aagttaacta
      181 ctttgcgtaa ttggtctaag atgctacgca ctaaggcttc caactcaact gtctttctaa
      241 tttgatcttc gattacctcc acctgggcag tgatgtaggg atcggttttt actatgctct
      301 ttaaccttat gcgggaaagc ccctgcacca gaaaacggac cgagccatct ggaaagcgga
      361 gcatcttgag tatggtggca gcagtgccca tcttgtagat gtcatccggg cctggctctt
      421 cctgctccgg gttcttctgg gttaccaatc caatcacctt gccgtgcatc agcgtgtcat
      481 ctatcatatg ggcatatttc tggctcgaga ttagaagggg cataatcaga aaaggataga
      541 ccaccacccc cttgagcggc aagatggata attcctcttg gattttcttt gtctcttctt
      601 gtggttgctt gatttccata ttgatttaat ctcctccctc gcgctatttc attccacctt
      661 aatctgcttt cccttcatcc tctcgtcctt cttcttgtcg atcctgattt ccaaaagacc
      721 gtccttatag ctagctacga tttcttccgt atccactgtc tccggaagat agatattacg
      781 ttcgaacatc ccgtagttga tctccatact gcgataacca ctcatatttt tgacatccct
      841 ttcttttctt tccccacgaa tcaccagcac gttatcagcc agcgagatgc ttaaatcctt
      901 ttgtttcatc cctcctattt ccaccaagat gataatttca cttgtggtct cgatgacatc
      961 tgccggcggt cgccataacc ttcgggaggt cagtaccggt gagtgtctca ccttgtaaaa
     1021 atggtcaaag agaatatcca tctcctcttg aatcttcttg atgtcgcgga tgatatgttt
     1081 cttaaaatca gccatatttc acctctttgt gaaactccac agctttacag ccgtggcttc
     1141 ttatgattct tcggcgaaac ccgccgaagc agaagtgcta gtatccgcct tcgccaaggc
     1201 tacggcggat acccgccaca ttcatcgtcc gacgccatag gctatggccg acggcgtcca
     1261 cgggttcaca cccgtggctt tctgcggagg cgggtaaatt cacaagttga acctgaaaag
     1321 gattacatct ccatcctgga ctatgtagtc ttttccttcc aactttaact cacctttttc
     1381 ccgggctctg gcagttgagc cgaactccaa gagtttccca taacttatga tttccgcttt
     1441 gatgaatccc ttttccatat cagagtgaac tgctcctgct gccttcaggg cagtgctgcc
     1501 ggattttata ggccaggcct taacttcgtt ttgattagcc gtgaagaaag ataccaagtc
     1561 taaaagctta tatgaattgt gggtcatctt atctagggct ttctccttta tgcctaactc
     1621 ccctctcagg ctctctttat ccttctcttc caattgagat agttccatct ccagcttccc
     1681 gcaaactaag catacatcca ctttctcatc ttcaatacag cttctcattt ctctttctat
     1741 tctggctgaa tctcctatct ggttctctcc tatatttaat aaaaccagca tcggtttcaa
     1801 agacaaaaat ccataacctc gggtgagttt ctcctccact tgagttaact tcaacttcct
     1861 taaagggatt ccaccttcca gggactcttt gaatctggcc aagagctcaa actcttcctt
     1921 gtcctctttt ctcccaactt tcaaattttt ttctaaacgg gatattctct tttccaccaa
     1981 atctaaatcc aaaagaacca aatcgcaatc aaaatcctta atatctcttt ttgggtctat
     2041 ctgaccatgg atatgaacaa cattttcatt ctcaaactct cttaccacct gcgccaactg
     2101 gtctgtttcc cttaagctag catagaattg cccttctgac cccttcttct tttcctcttc
     2161 aattaagccg ccaacatcaa tatattccac ttccgttggt gtgacctttt tgggttgata
     2221 aagttgagct attttgtaca atctttcatc cggaaccttg actaccccta tattgggatt
     2281 tttcgggcct aaggcgaagt cgccaatttt tgctttgcca ccagttaaag cgttgaagat
     2341 agtggtcttt cccactaagg gaagacctac gattcccact ttcatatttt gatttgactc
     2401 atgtcattta cccttcatta caagcctatt ttacatcttc agcttccagt tgtcaatcat
     2461 tttagagata aagctttctt tatgtcttga tataccgagg acacaaatca cctcaaacca
     2521 aatatcatat tgatactttt gttgtgtcgg gatctactcc tgacatattt cctaaaatct
     2581 gtaggcgacc catctgggtc gcctgcgaaa accaaacatt tctgaaacat tacagtttta
     2641 tcaaaatctt tttttctaaa ctataaactt tgaacctcaa tttcttgaac tcctccccgg
     2701 ctttagagtt gaccagtttc cccaactcct ccaaaaccct cagaccttcc tgagccctta
     2761 ttagattggc ctggactacc ccttttagcc cttccctttt tttctctaag cccctcatcc
     2821 cctttgctcc aacatccttc tggctatctc gacttttgat gagctggtct tggtctaaaa
     2881 accttatcgc tattttactg atttgatgtc ttatcacttt catcttagcg gaaagatttt
     2941 catcatttag ataaagccga gccacttcct ctaccacgcg caaaccctca cgcgagcgat
     3001 tcaaattagc atctatgagc ctataaattc ccttgtccat cattttcacg aaaatatgcc
     3061 acggtgcccc cgtagggggt gaatggcatc cttttggttt cctctccctc aagggagagg
     3121 aaactgtttt tcaaacagga taagatgcct cgccccttcg gcggggagtt tcacttatga
     3181 gtgaactctt tataaaaagg tgactttacg acttcggctt tatacccttt tcctctaatc
     3241 tctatatcaa agttggctcc gagtgtggaa tatccagtct cgacataccc cattccgata
     3301 cccttatcca gagatggaga aaaaactccg cttgtgactt cgccaatttt actggtatct
     3361 tttagaatgg aataatgctg gcgaggaaaa cctttttcta aaagttcaaa gggcactaat
     3421 tttctttttg acccttcttc tttcattttc aaaaatgact cttttccgat gaaatccccc
     3481 ttttccggcc tgactatcca ggaaagcccg gcttcaaacg ggttggtggt cttgtctata
     3541 tcattgccgt aaagcatata tttcatctcc aaacgtaagg agtctcttgc tcccaagccg
     3601 attggctcga tttcaaactc ctttcctgct tgcatacttg cattccaaag attctccgca
     3661 tggcaaaaag gaaaataaag ctcaaaccca tcctcacccg tatatcctgt tctggagaaa
     3721 agcatatcta caccactcac ctttccgaaa gttgcccaat aatatttcca tttgcttaaa
     3781 tcgacattgg tcatattgga taagactttt tcagcatccg gaccttgaat tgctaaaagg
     3841 gcggtctcat cacttttatt tatcaattta acgtctccaa atagattgct ctgtagccat
     3901 ttaaaatctt tgtcaatatt ggaggcatta accactaaca tataataacc gggcaactta
     3961 tacactaaaa ggtcatccac aatcccaccc tccgcatagc acatacagct atattggact
     4021 tgataggtct ctaatttgga aacatcattg gtagtgactt tctgcaaaaa agccagagca
     4081 tcttttcccc taacttcaaa ttcccccata tgggttatat cgaagatgcc gacgcttttg
     4141 cgaactctct tgtgttcatg ggttatgcct tgatattgaa tgggcatcaa atatcctgca
     4201 aactcaacca tcttggcgtt atgcttcaga tgtaattcgt aaaaaggtgt ttttttaggc
     4261 atgacattcc tttcattcaa gaagattttt caatctgggg ataaagttcg tctatgattt
     4321 tttgaatttg gatggtcctg tcaagaacag ttacaacttt gcagtaatgc ttaatatctt
     4381 caagggataa tttccgtttc tttctatctt tcaaccactt acttaacact tgatagccac
     4441 caatttgata ttcccaaatc tctcttccta tgctttcaaa atattggtcc ttgtttatat
     4501 aaacctttcc tttttcaaat ttaatttttt caacgctgtt atctcccttg ccttggaatt
     4561 tgggtagggg cgattcatga atcgccccta catgaattgc ccctaccaga tgcaaatcaa
     4621 ccaactcttt cccatattca gccattttgc taaatagctt gtaatctttt gtaaaaggaa
     4681 ttctcggaaa atcaatcttc aaaaactcag aatattttgt gcggtaaata ttggaataaa
     4741 gaacggcata gatgtaatag aaaacctctt ctggtgttgg ttcttttcta taaacttcag
     4801 tgagtgtagc gaaaactttg ggatttatat ttggctgtcg tttctctgat tttttctttt
     4861 ggctgaacag gtcttttttg tccgtgtcag ggtagaggta gagaggaaaa tttacgttgc
     4921 caccacgata gaacaagttt agatctccaa accattccga acagaagact atattccaac
     4981 gtttttctaa tcctacaact tgtcctgctc tacctacgca taaggcaaga ttctcttgta
     5041 tcatatggcg catgacttcc ttgcgcgaac gttcgacaac ggcatcgtgg tagaaaatcc
     5101 accggatgtc aaaggggcgg tatagaattg ataggatgaa tctactcaag tcggagtcgg
     5161 aaatggagtg aatggccttc caagcctttc gaatatccca ccctttgctt ctatttatct
     5221 ggaaaaacgc atgaagttcg tcatcagaat atttattatt cttgaaaagc cggattctat
     5281 tcagaaggtt gtctttattc atgtctatgg caaaaccgtc tcttgctgta aataagccta
     5341 cactattcaa tggaaatagc tcacttattt taggatgttt ctcataatct tctaagagct
     5401 tttcctctct cggaacaaag aaataataag gactggctgg atttagcttt ttccattttg
     5461 ttgtttttat atcatttttc acaagccagt catattttgt ttctcttaat ccccaaacct
     5521 ccgaatgaaa aaccttacat ttgttactat tgctcataaa taccttcaaa atatttttta
     5581 tgctctaaat tgtaattagc agaaaaggga ttctctctat ccaattccca tttcaagggg
     5641 ttattcgtga tatattctcg aatattgcct aattcctttt cactacggat aacgtgctca
     5701 taataatttc gttgccaaac cggcataccg caagaattct gcatttgatt gattttcttc
     5761 gcacaattca ttttgaaata tccaatgatt ttgggaatta acatcttcct acggcgaatg
     5821 cgtaggggtg gttttcgtag gggcaattca tgaattgccc ctaccttctc aaccattatt
     5881 atggtatgta tatgatttgg cataatcaca aattgatcta acttcgcatt tgcaaaatgt
     5941 tttgaaatgt caaataaata ttcctgtgca atctttccta tgcttgacaa ttccatattc
     6001 ccgcccacca ctttacccaa tgggtatttt ttatcttgcg tgcatatcgt cacaaaatat
     6061 gcgccctctt gcgaataatc ataacttttt aatcttattg aatggcgatg atatttttcg
     6121 gggttatttg tcatttctca taatattcta cattatctat aagacctttt aagtttgatt
     6181 tgaaagaaaa taactttttt tcacccttac aaccaaaaaa taaaatgccg tcaacattag
     6241 attgaaactc actcttaaga ttgggatatc tgtcagtgtc ctcaaaataa cttgagtcta
     6301 tcgaccatct acctgaatcc actgcaaata ttttgtaatc cgccttgcag ttactaagct
     6361 gcgatttttt ccctattaac ttatttaata atccagcaaa acttttgtca gcaaactctg
     6421 tgacattttc tatgttagca acacctacta tctcaccttg atcctgcaaa agctttcctt
     6481 ccataagttg ttcgatagga gaccctttgg atcgcatcct tgtcacctca acacaaatag
     6541 agacattttt aggcccaatc cttattctta tatcaggtgt tccgtccttc ccttccttaa
     6601 taaactcaat aataggttgt ggatggatat ttttaaacaa gaaatgagca atctgtaatt
     6661 cagcaagata gtccaaaaat tgctctatac ttgaagcatc ccaaaacttt tttcctagaa
     6721 ttttaccact ctgtttgtct attttctgat taacaaatgt aaaataatct tccagttttg
     6781 gcgaagggca aaaatcggga tgctctataa tttcattcca agctgagcat tttaaatctt
     6841 taaaaaactc gtatgtttgc ggaaattctt tggaaaaaga tgatttatcc atatttattt
     6901 gcgtttaatt aggaacacaa tcgctactcc ctgttgaata tcaaaaacat tttcgtcttt
     6961 acttccatcc ggacatttct cttttttcaa actgttgccg tgcaaatcta aaatataaat
     7021 ttcattaaaa ctgttcatca tggattgcct catccctctg aaagttggat tgtctaaata
     7081 actgtgattg gtgataaatc ccaaaacccc ttcacccgct tggtcaatct tccactgggc
     7141 aaaacgaatg aacttcacat aatcatcctg aagccatttg ggattttttt caccaagtgg
     7201 tttcccatca acaaatttgt aatcttctat gagattacca atccaagttt tggtttttct
     7261 ttttctttct ttgccatttt tcatctcaac aatatatttc tcactcacat ttgcagaatg
     7321 ccctgaatat ggcggattgc caagtataac caaaatgggt gtctcttttt tcacttttcc
     7381 tgccaaatga gattcctcgg aaagggaagc cataccgggg agttcggttt gagtgagttc
     7441 ctccatctcc agggtattgg taaggtagag tttaaatcta tcatcttttt ggagtttata
     7501 tccaagctct tctaacagaa aagacatctt taagtggcct actgcataag gagccatcat
     7561 caattcaaaa gcatagaaat ccttgagaat gtgttccctt attagatttt cttttccgcc
     7621 cttaccatat tttcctacaa actcatccac tgctattttg gatgcctctg ccaagaaggt
     7681 aagcgttcct gcagcagggt ctaaaaccgt aaccgactca cctgcaaatc catcttttct
     7741 gttgaaatgt tctttcaaaa tattatgcaa agaacgaaca atgtaagaga ccactggctc
     7801 tggggtataa tagacgcctc ttttttctct tgtttttggg tcatattcag tcaaaaatgt
     7861 ctcataaaag tgaacgatag ggtctttacc tttgccttca tgaaaatatt gctgcaatat
     7921 cttttttaca tcagtatttg caaggatttc cgaaatatca tcaataatcc attccatctg
     7981 ttgcggcaag tcctcaagag aaatgaattt aaatacatct ctcaaaatcc cgatagtgcg
     8041 cggaatatta tcgtaggcaa gttttctatt aaagccgttt tctgagcggg tgcgggccgc
     8101 aaaaagcccg tatgtaatgg tttgtgaata aaggtcagca aactcttcct tagtaagacc
     8161 agctatgaga tattgcctga atgcctcata gaaccccaag ataaaacctt tgccattctt
     8221 ttcttcttct tttaactcct gtgcaatgac ttcatccttc aaaaagcgag tcctctttgc
     8281 taattcaatt gccaaatctt tggcatcata aacttttggc aaagagaagg agaagaactt
     8341 ctcgagtaaa tcaaagaatt cagattctct ctcagctatt ggaatggttt tgagtttatg
     8401 tataacaaat ggtctggcaa ttaaaacttt atccatcaga gaaccattgc gataaaatct
     8461 gaattcacaa aaattggtca agattaagtt tgggaaggtg tgaagataac gttttaattg
     8521 ttctgagtct tctatttggt ctaaatctgc agtcgttggc gctttggctt caatatagcc
     8581 gacgatatgt tgcttaccat cccaaactct gaaatcagga ttgccagctt cggttctctt
     8641 tggctgaggt gttatgtaga tattctttct gccaattgat tccgcatatt catttaacaa
     8701 ttccttaaga atggaataat aactttcctc tctggcatcc ccacgattgg cgatttcaaa
     8761 tatttcttta atgtattttt gtaacataga ttccatcaaa atatcgtttg agttccaaat
     8821 tataattggt tgaaaaggga ttctctctat ccaattccca ttttagggga ttggtaataa
     8881 tatattcgcg aatacgattg agttcggctt catttctgat aatgtggtca tagaaacggg
     8941 attgccatgc aagcggatgg gaaatatttc tacttctgtt aattttgcgt gtcaccccgg
     9001 atttaaatcc tgcaacaata taagacaacg attttacaat cacattccgg aatctatttt
     9061 gccttcgtag ggacagatta taatctgtcc ctacagatat cctctgattt attataatta
     9121 tgccataaac atgatttggc ataacaacaa attcatccaa ttgcacatct ccgaaatgtt
     9181 ttggaatttc aaaccaacat tcttgcgcga ttttgccgat ttttgacaac tctacatttc
     9241 cgtccataat ctctcccaat agacattctc tttgatgtgt acatatggtc acgaaatacc
     9301 cgcctgcttg ggaataatca taacttttca atcttattga accgcgacct cgtctcttgg
     9361 gggatatttt cactatctct aatttcaaat taggtttttc aatttaatag ttttttcaaa
     9421 aactgaccgg tataagaatt cttatttttt gccacctcct ctggtgtgcc gattgcaacc
     9481 acttctcctc cctcgtctcc accttctggt cccaaatcta tgatatggtc ggcggtcttt
     9541 atcacatcca gattatgctc tatgaccaag accgtgttgc ccttatccac caagcgattc
     9601 aaaacgccaa gcaacatctt gatatcatca aaatgcaagc cagtggtagg ctcatccaaa
     9661 atgtaaaatg tcctgccagt tgagaccttg gacagttcag ttgaaagctt aaccctctga
     9721 gcttcccctc cggaaagggt agtggcggct tggcctaaat gaatatatcc caagcccaca
     9781 tctgccaggg tctcgagctt tcttctgatc ttgggaatat tcctaaaaaa gcttaatgct
     9841 tcgcttactg tcatgtccaa aacctcggcg atgttctttc ctttatatct tatctccaag
     9901 gtttcacggt tgtacctttt tcctttacac acttcacagg gaatatacac atccggcagg
     9961 aaatgcattt cgatttttat cattccatcc ccctcacaag cttcgcacct tcctcctctt
    10021 acattgaagc tgaaacggcc aactttgtat cccctcactt tggcttcagg tatttgggcg
    10081 aatagatctc ttatgtaagt gaaaagtccg gtataagtag ccggattaga gcgaggggtt
    10141 ctgccaattg gagactggtc aatgtctatg accttatcta tgctctccag tccttttatt
    10201 tcggaatagg gtaagggagc ttccctggaa ttataaaaat gagatgccag ggcacgatat
    10261 aaggtctcgc ccacaagact ggatttgcct gagccggaga ctccggtaat gcaaacgaat
    10321 attcccaaag ggatattgac tcttgtgtgt ttcaagttat ttccacaagc accgattaag
    10381 ctcaggaatt tcccatttcc ccttctccta tcatctggta taggtatgga gagttttcct
    10441 gaaaggtatc ttccagttaa ggatttactg tttctcttta tatctttagg tgctcccaca
    10501 gccaccacct ctccaccatt tatccctgca cctggaccta agtctatgac gtaatctgcg
    10561 gtttgtatgg tttccctatc atgctccacc accaatactg tgtttcccaa atccctcaag
    10621 cccagaagag tattaagtaa ccttttgttg tccctttgat gcaaaccgat ggagggttca
    10681 tctaaaatat aaagcactcc caccagacgc gaacctatct gagtagctaa cctgatcctc
    10741 tgtgcttctc cacctgccag cgtcccagct cgtcggtcca aagtcagata atccaacccc
    10801 acatccacta aaaaccccaa cctctccttt atctctttaa ggatctgttt agctatttgt
    10861 gtctcccttt cagagaagga aagattggcg aaaaaatcct tagcctgctt aatggaaagt
    10921 tggctaagtt gatttatgtt taaacctcca actttgactg acaggctttc cttcttaagc
    10981 cttgctccat tgcactcctg acagggtctc acccgcatga acctttcgat ccattccctc
    11041 actccttcgg actgggtctg tctgtatctt ctttctaaat tgggaatgac accctcaaac
    11101 gaatcgaaat attccccttt tccctttctt tccatagact cccaatagaa tcttatctcc
    11161 tcacggcctg aaccaaagag aataacctgt tgggctttgg aattaagttg tccaaaagga
    11221 gtataaaaat tgaaaccgta atgctcagcc acgctcttca aaagatgata gtaatagttc
    11281 cctaccggta ttccccagca ggcaattgct ccctgactga ttgagagatt tttatcggga
    11341 atcaccagat cagggtcaac gttcatagtt gttcccaaac catcacatcc cggacaggct
    11401 ccaaaaggac tattaaagga aaacatccgc ggagtcaact cttcaaaact tgtgccgcaa
    11461 tgagcgcagg cgtacttttc tgaaaagact aattcctctt ttcctcccac gctcaccagc
    11521 acagtgcctg ctcccatctt taaacctatc tccagagaat cagccagcct tttgattatc
    11581 tttttttcca ccactaacct atccaccacc acttctaggt tgtgtttctt gttcttatcc
    11641 aaagcaatcg ggctatccac atccaaaatc tttgcatcca ccctcacccg aacaaacccc
    11701 tcctttttta tctcctcgaa aagttcttta tactcacctt tcctgccccg aaccagcggg
    11761 gatagaatct gaatcttact tcccggaggt aacgacaaaa ctgtatcggt tatctgctgg
    11821 acagtctgct gggagatttt tctgccgcat ttgtagcagt agggaactcc cactcgggcg
    11881 aacagaagtc tcaggtaatc gtagatttcg gttactgtcc ccacagtgga acgcgggttt
    11941 ctgccggagc ttctctgctc tatggatata gctggggaga gcccatcgat gaaatccaca
    12001 tccggcttct ccatcattcc cagaaactgg cgagcatagg cggagaggga ttccacatat
    12061 ctcctctgcc cttcagcgta aatggtgtca aaggccaggg aggatttgcc tgagccagat
    12121 attccggtga taaccactaa cttgtcgcgc gggatatcca ggtctatatt ctttaaatta
    12181 tgctctcttg cccctctgat tttgattatc tgttgtgaca tatttttcta aaagattaac
    12241 tttataatat acgaaggatt tttaccctcc gcaagcacac ttttgaacaa aagactatcg
    12301 gatttccggg gtgaaggaat tattttagtt gctttttgag ttgagagatt ttatcttttt
    12361 aagttaaagc tatgggctca attcgagaga atctaaagat agtaagggag aggatagacc
    12421 aggcggcctc gaaagctggc agtcgagggg atgatataaa attggtggca gtcactaaaa
    12481 acgtcgatgt ggagaggata aaacaaatta tcgcttgtgg gatagagatt atcggggaaa
    12541 atagagttca agaagccctg tccaagtttg actatctgcc agctgaggtg gaaaaacatt
    12601 tagttgggca tttgcagacc aataaggcaa aaaaagcagt aagacttttt gatatggtcc
    12661 aatcggttga cagttcagat ttagcttttg agatatcaaa aagagctaac gaaataaata
    12721 aagaagtccc catcttaatc gaggtgaaca cctctggtga ggagagtaaa ttcggcatag
    12781 agccggaaga ggctttaccc ttgatagaaa agatcggcgc tctggataat ataagaatag
    12841 gggggcttat gactgttggt ttatggagtg gcgaggaaag caaagtcagg ccttgttttg
    12901 tgaaactaag gaagcttttt gaaagaatga aggagcagaa tatacctgac gtgcaaatga
    12961 aatatctatc catgggaatg accagtgatt tcgaatgggc tatcctagaa gggtcaaata
    13021 tggtcagaat cggaactgga atatttggtc ccagggtagt ctaagattta aatctttttg
    13081 tcgataaaag tagaaaatcc aattctggag ggagaaatga aaataactcc tttggaaatc
    13141 aagagtcagg aattcggcaa gagcttcaga ggttatcata cctttgaggt ggatagtttc
    13201 ctggataatt tggctgtgga gatggaagaa ctggtaaggg aaaacaacct tcttaaagaa
    13261 aaactcaaag gtctggagga aaagttaggc gaatacaaaa agattgaaaa tagctttcag
    13321 gaaatgcttt tgatcacaca gaagtcaact gaggagataa agaaaaatgc cgagaaggaa
    13381 gccgatttag tggttcgaga ggcggaagtt aaagccggga agatattgga ggagactttc
    13441 ggccgacttt cagagctcaa gaaccagata gccaatttag acagcttgaa gagaagttat
    13501 gttctgaaac ttaaatctgc cctaaaagcc cagcagcaac tgctggagga gattgaaaag
    13561 gaggacattt ccacaactaa attgaatttc agacgaaggg ctgatctcac cgaggaggag
    13621 cttaaccggg tagtggaaga gttcaagaaa gaagatgact taagcccaga agaaacaaac
    13681 taaacggagt accagatgga tgatttaaaa agcaaaatca atcaggcagt tgatttcata
    13741 aggagtaaaa cctccttaaa gccccagata gggataatcc tgggaaccgg cttgggctct
    13801 ttgactgaga tgataaaaca agagattgtc atctcctata
//
LOCUS       DASWLN010000187        14795 bp    DNA     linear   ENV 20-FEB-2024
DEFINITION  MAG TPA_asm: Candidatus Zixiibacteriota bacterium isolate
            SMAG_U2949 k141_1259632, whole genome shotgun sequence.
ACCESSION   DASWLN010000187 DASWLN010000000
VERSION     DASWLN010000187.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37960594
            Sequence Read Archive: SRR10376091
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Candidatus Zixiibacteriota bacterium (soil metagenome)
  ORGANISM  Candidatus Zixiibacteriota bacterium
            Bacteria.
REFERENCE   1  (bases 1 to 14795)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 14795)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (26-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/12/2024 08:28:26
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 1,440
            CDSs (total)                      :: 1,416
            Genes (coding)                    :: 1,412
            CDSs (with protein)               :: 1,412
            Genes (RNA)                       :: 24
            rRNAs                             :: 1 (16S)
            partial rRNAs                     :: 1 (16S)
            tRNAs                             :: 21
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 4
            CDSs (without protein)            :: 4
            Pseudo Genes (ambiguous residues) :: 0 of 4
            Pseudo Genes (frameshifted)       :: 0 of 4
            Pseudo Genes (incomplete)         :: 3 of 4
            Pseudo Genes (internal stop)      :: 1 of 4
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..14795
                     /organism="Candidatus Zixiibacteriota bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_1259632"
                     /isolate="SMAG_U2949"
                     /isolation_source="soil"
                     /db_xref="taxon:2053527"
                     /environmental_sample
                     /country="USA: Oregon"
                     /lat_lon="42.99 N 123.63 W"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            23..1393
                     /locus_tag="VGB16_06915"
     CDS             23..1393
                     /locus_tag="VGB16_06915"
                     /inference="COORDINATES: protein motif:HMM:TIGR00797.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="MATE family efflux transporter"
                     /protein_id="HEX9917454.1"
                     /translation="MNDQATNSKIDIDLTSGSITKNIFKLAWPVMGSMLIETLRTIAN
                     AFWVGKLGAVALAAVISSAFMIWIIYSFISTVSVGVVAMVSRFVGAKDKESATYVSRQ
                     AYLFSIFSSLTLMITGIALTPYLFSLMGTEALVTQAGIRYLRIIFLGSIFFFLIDVFS
                     SIFRASGDTKRPLWVTLFSLGFNIVLDPFLIFGWGPFPRLETSGAAIATVISQCLGVL
                     IFFYLIKKGKLLFKFSLKPALKVDFSIIRRIVRIGIPPSIAGIVFSIVYLFLNRIVAH
                     FGTESIAALGIGNRMESLSYLSCFGFSIAAATLVGQNLGAGKPERANKSAWRTIYITT
                     FITGFISVLFLSFPKAISSFFITDPKVVPIAINYLRILSISQIFMAWEIVLEGAFSGA
                     GDTLPPMIVSLPGSILRIPIAYLLAITLGLGVNGVWWAITLTSVVKGTVLVFWFRQGK
                     WKKRQI"
     gene            1481..4063
                     /locus_tag="VGB16_06920"
     CDS             1481..4063
                     /locus_tag="VGB16_06920"
                     /inference="COORDINATES: protein motif:HMM:NF017044.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0046872 - metal ion binding [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="pitrilysin family protein"
                     /protein_id="HEX9917455.1"
                     /translation="MLSNIERMTLKNKMVVLLKPDSSSPLATINLRIKSGFLNEPDEL
                     TGISHLLEHMLFKSTKKMETAQLKEKFRHLGGVFNGETMYDYTMYYCTVPKNNLRAAL
                     ELEADLVYNSLFDRGELEKEKQVVIQEIRRKDDMLSIFAWEKMMENSFVRHPFRRWRI
                     GTTENITEMKREDILRFHNENYSPRNTILTITGDFESDKVSHWVREYFEKLRPLPPKK
                     LSFPIEPEQTSLRYQSLKGDLKQTYLKIGFHAPGVLEEDNFALVILSYILGQGKSSRL
                     SQILKARQGLVNSISSDLYNLKDLGVYFFEVELEDKNILPVLIEVFRQIEKIKQSKLS
                     KAELARAKNLWRADFLSNFESLAGQADILSLFEQYGDYRLIQKLMHKIEQVSKKDIQK
                     AAKRYLNLDKATVLRYAPQKDKTALESLRRKIILGIKAKGPEFKPVKSISFKKIGSAQ
                     KEGKSLNTELLNYGTYLLTREKHTLPMVTVDFYFKGGRNDENEGNCGITRLCLENVLK
                     TKSKGESLTSQLEVLGGTLSTTAEPDFFGYSMEISAENLDGALGIVFEILQHPTFEKK
                     IADIERKLLIAEIEKSQDDMLNYPVHLLKRTIFPGHPYAKSLWGDERSLEKITLKQLE
                     NWHKTYYIRQNLVISAVGDFKTEWLKKKIELGLKSLGRVKIEEQKMILVHQTKERTNI
                     VPRSKNQTAIACGFLTCGFADDDLYPLKLIQGIFSGMGGRLFTELREKRGMAYAVYAA
                     CDAYKNGGIFYGYIGTSPQKEKIVIDLLKQEFEKIGKRKVEADELEKSKAYISGAYNI
                     SLQTNSALAHQYAKNHILGRDAHQAEEFGQRIDKITSREILHIARKYFDLNNFYLGMI
                     RGRK"
     gene            4060..5004
                     /locus_tag="VGB16_06925"
     CDS             4060..5004
                     /locus_tag="VGB16_06925"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_013249736.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="histone deacetylase"
                     /protein_id="HEX9917456.1"
                     /translation="MMSTGLVYHPDYLLHDTGAGHPENKERLISLMQHLEKTGALSQL
                     KLIQPNSNLALEWVKKVHSPDYIELVKQTCEKGNGMLDPDTVVSKDSFNVALLAVEGV
                     LQGVEEVMGSNLKNVFCAIRPPGHHAEKERGMGFCIFNNLAIAAQYLLEKHKLNRVLI
                     VDWDAHHGNGTQEIFCSDPRVYYFSIHQFPYYPGTGKENEEGENKGKGFTFNMPMAAG
                     SGDLEYVEAFENILSPLALKFSPEFILISAGFDAHQDDPLTGLKVSTKGFKKMTQVTK
                     KLADSCCQGRIVSALEGGYHLKSLCDSVETHLRVLMED"
     gene            5009..5773
                     /locus_tag="VGB16_06930"
     CDS             5009..5773
                     /locus_tag="VGB16_06930"
                     /EC_number="3.7.-.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_018249307.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003824 - catalytic activity [Evidence
                     IEA];
                     GO_function: GO:0016787 - hydrolase activity [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="fumarylacetoacetate hydrolase family protein"
                     /protein_id="HEX9917457.1"
                     /translation="MKRFARFKIEDKEHYGIVEEKIYPLEGNIFCQYEVSSYGYNLDE
                     VKLLPPCQPTKIILVGLNYHDHIKESSSASQAPEEPVIFMKPTSSLIATEEKIIYPPG
                     VKRLDFEAELAVVMKERCKDTSAEQAGNHIFGYTCLNDITARDLQTKDIQWTRAKSFD
                     TFCPLGPFISVGIDPSNLKIESYLNGKVQQSSNTNQMIFSVEEIVSFVSKVMTLLPGD
                     VISTGTPSGVNSLIPEDTIEIRIEHIGILRNHIMPA"
     gene            5883..7400
                     /locus_tag="VGB16_06935"
     CDS             5883..7400
                     /locus_tag="VGB16_06935"
                     /inference="COORDINATES: protein motif:HMM:NF024878.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="HD domain-containing phosphohydrolase"
                     /protein_id="HEX9917458.1"
                     /translation="MSKAPNILVVDDEKYICDIIKESLSDFDYNVLVTDSLQKAIQII
                     ESQPIDFLLTDLILGDKSGMEVLNKAIELHPDVVVVLMTGQPTIENAVSVLKLGAYDY
                     LIKPFSVERLKAIIRRGLERQQLYRENVHLKEMVSLYQISQAMGSTVELDSLLNLILD
                     TAVREFGANLGSILLWDEKAKSLKPKASLGTTKGKSNQRDLSEEDPIIDSIIKNGQPQ
                     ILDESNIRQMLSSETKGRDIKSAISHPLLAKGKVIGVLNLIRSQRANPFTKGQLQSLS
                     IIASEAASAIENSKLYENLKGSYLETLTALANAVEARDTYTRGHTERVWYIAEFLAGQ
                     MGWEEERMREVKMGGILHDIGKIGVPDAILNKPGKLTLEEFEIMKLHPQQGVKILEGI
                     SFLAPALPYVLYHHERYDGQGYPEGLAKEDIPVQGRLMAVVDTFDAISSDRPYRKSKG
                     FEKALEEIKENAGTQFDLSIVEVLLDAWNKGLIDKGKLEVQTSKESFASSAKVKS"
     gene            7575..8165
                     /locus_tag="VGB16_06940"
     CDS             7575..8165
                     /locus_tag="VGB16_06940"
                     /EC_number="1.5.1.-"
                     /inference="COORDINATES: protein motif:HMM:NF013756.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0010181 - FMN binding [Evidence IEA];
                     GO_function: GO:0016646 - oxidoreductase activity, acting
                     on the CH-NH group of donors, NAD or NADP as acceptor
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="flavin reductase family protein"
                     /protein_id="HEX9917459.1"
                     /translation="MKKIKEKIDFDINQLSTPFPRLGELRFPLLGQIVLVTSISREGI
                     PDVAPKSWISIFSSNPPIVGFGCNTNHTTAKNILSTGEFVINIPGEEIVQSAWKIIEL
                     DKDDPKKIEKAELTLTKSTKISTPRIAECKAHLECSLDWTKKYKEEIVIFGKVLLAAM
                     DKEAVEKDVDERYKYFKLLAYLDERTYGVINAAKKV"
     gene            8168..9052
                     /gene="htpX"
                     /locus_tag="VGB16_06945"
     CDS             8168..9052
                     /gene="htpX"
                     /locus_tag="VGB16_06945"
                     /EC_number="3.4.24.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_012583272.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004222 - metalloendopeptidase activity
                     [Evidence IEA];
                     GO_process: GO:0006508 - proteolysis [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="zinc metalloprotease HtpX"
                     /protein_id="HEX9917460.1"
                     /translation="MYKQISANKRNSFFLVLFFIVFILCLGWVFGQAQGWGYAGLVVA
                     FVIALVLALIGYFQSDKIVLAVSKAKPVDEKENPYLANVVEGLAIAAGIPKPKAYIIE
                     DSAPNAFAAGRNPKVAAIVVTTGLLDKLDRLELEGVIAHEMSHIKNYDIRYATLVVIF
                     VGTIVLISDWMGRSFFYGRRRRDSRGGQIEGVIILVALALAILSPLIAKLVQLAISRQ
                     REYLADANGALLTRYPEGLASALEKIAKDTEPLEVANKATAHLYIVNPLLDHKGKLNE
                     LFSTHPPTEERIKRLRFM"
     gene            9066..10340
                     /locus_tag="VGB16_06950"
     CDS             9066..10340
                     /locus_tag="VGB16_06950"
                     /inference="COORDINATES: protein motif:HMM:TIGR03991.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="putative sugar nucleotidyl transferase"
                     /protein_id="HEX9917461.1"
                     /translation="MRQKVFIFEDAAYANFLPLSYSRPVYKLLFGINTLEQKISLLFP
                     EAEITLLCRDYLKDWIGTKTQLKVNSFGVEEADQILLINGRAIFDESLRSNLSFSDED
                     KVYLCAKEVVAISLKGSSFKKYKEKLVQLYEPQNLDHIKGQLKNVRIKAEMASYLWNL
                     VERNGEQIKKDFDLFKKNSKFKTNRKASQIDSQAVLYNDKEIFIGEQSQIDAFAVLDA
                     RKGPIYIDRKVTILSHTRIEGPCYIGEESQILGGAIREGNSFGPVNRIAGEVEKSIIL
                     GYSNKCHHGFLGHSYAGEWVNLGALTTNSDLKNNYSSIRVMLNGEMVNTGLSKVGVFL
                     ADHVKTGIGTLLNSGTTVGYSTNIFGGGMPKEKFIPSFYWGGKDGWVKYQLEKAIQTA
                     EITVMRRQVKAGENLKELFHKIFELTEKEKEL"
     gene            10411..11409
                     /gene="galT"
                     /locus_tag="VGB16_06955"
     CDS             10411..11409
                     /gene="galT"
                     /locus_tag="VGB16_06955"
                     /EC_number="2.7.7.12"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_015743620.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008108 - UDP-glucose:hexose-1-phosphate
                     uridylyltransferase activity [Evidence IEA];
                     GO_process: GO:0006012 - galactose metabolic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="galactose-1-phosphate uridylyltransferase"
                     /protein_id="HEX9917462.1"
                     /translation="MPQLRKDPVIGRWVIISTERGKRPSDFPAPPKRQESKLCPFCPG
                     NESSTPPEVLAFRPDQSPANKPGWTLRVIPNKYPALKVEGELNREGRGIFDKMNGVGA
                     HEVIIETPEHTKELADLDQKRVEDVLWAYRLRTVDLKNDPRLRYILIFKNQGLEAGAS
                     LEHSHSQLIATPIIPKRVSEELDGSKKYYDYKERCIFCDIVKQEQEENVRVVNQNESF
                     ISIEPFAARFPFETWILPKKHYSTYDDTKQQEMPALAAILKDTLSRLAKALNNPPYNY
                     TMHTRPVGDSFTKEYHWHIEIMPKLTKVAGFEWGSGFYINPTAPEDACNYLRSIQL"
     gene            11445..12935
                     /gene="glgA"
                     /locus_tag="VGB16_06960"
     CDS             11445..12935
                     /gene="glgA"
                     /locus_tag="VGB16_06960"
                     /EC_number="2.4.1.21"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_012500214.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004373 - glycogen (starch) synthase
                     activity [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="glycogen synthase GlgA"
                     /protein_id="HEX9917463.1"
                     /translation="MLKEKLKIAIVSSEVVPFAKTGGLADVAGALPKVLSKLGHEVKV
                     FLPKYKMVDDQKFNLIDKKIELSKIPIGEKQIELGLRSAMLADSKAEFYFLVNNFYYL
                     RDEFYRDNSTGLDYIDNDERFIFLARGTLEVLKKLDWQPDIIHCNDWQSGLIPAYLKT
                     LYKDDPFFKNTATLFSIHNLAYQGNFPSETFDKLGLERSLFYPASPFEFWSKVSFIKA
                     GIVYTDIVNTVSPTYALEIQSGSEYGYGMEGVLSSRKDDLFGIINGIDYDIWNPKTDE
                     LIPYKYDPDDLSGKEKNKEYLLKTSKLPLDKKRTPLVGVISRLADQKGFDLIAQISER
                     LLSLDLKIVILGTGDEKYHLLFTNMQKKFPHKLKVTLGFDNKLAHLIEAGSDMFLMPS
                     RYEPCGLNQLYSLKYGTVPIVRHTGGLADTIANYNPITGKGNGFSFKTYSAEKLLETI
                     QKAINLYKSETLWTKLTLAGMKQDFSWKASAKKYVELYQRALSKKW"
     gene            complement(13157..14554)
                     /locus_tag="VGB16_06965"
     CDS             complement(13157..14554)
                     /locus_tag="VGB16_06965"
                     /inference="COORDINATES: protein motif:HMM:NF037531.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="HEPN domain-containing protein"
                     /protein_id="HEX9917464.1"
                     /translation="MTWQFEYSSMWWLPEKPEMRVPGIIKLYQGKEITLELTYSSENK
                     EKIFTEISHDLILGTSPDGQLITLYKCSHAEHLTSLHSLEKATFEVDIVFIGVHFERK
                     EDIKFNQLFIYYLYLYEWARISSLETRFLPHKKGEIAKYERLKSIQMADFKDFKIFLG
                     LNPLLIREPKKINIEENAYIGIRAPKGIYFEEYLDIIYAIQSFLSFAVSSGTHPSGIA
                     GITNTRSEVSKNGEQVYYSINIICSPKIIRFASEEPIPNRMLFTFKDMNSKQHIFLEN
                     WLNKEDLLKPVYDLYLTQLYEPFLHKEQIFLNLALAIESYHRRTMNNFELSIQKHAKR
                     INEIIKSTPCPHKKWLEQKLNYSNELSFRGRIKDILRRFNKISKCFIGDKKSFIQKVV
                     VTRNYLVHLDKALKGKSAKGKELHNLNQKIKILIELCFLREIGFAPVKIRKLILEKYW
                     SLFIDFPNLDRRIFD"
     gene            complement(14613..>14795)
                     /locus_tag="VGB16_06970"
     CDS             complement(14613..>14795)
                     /locus_tag="VGB16_06970"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="RNA-binding S4 domain-containing protein"
                     /protein_id="HEX9917465.1"
                     /translation="DGIPAKASHNVRAGQKIQIDFSNRLLEAEILSVPPETNLTKEEA
                     KNLYKVIREERKRVLE"
BASE COUNT         4495 a         2905 c         3300 g         4095 t
ORIGIN      
        1 ggaataagaa cttggcccac ctatgaatga ccaagcaact aactcaaaaa ttgacattga
       61 cctaaccagc ggaagcatca ccaaaaacat ttttaaatta gcttggccgg taatgggttc
      121 gatgctgatt gagaccctga gaaccatcgc taatgctttc tgggtgggga aattgggtgc
      181 agttgccttg gctgcggtca tatccagcgc ctttatgata tggataatct attcctttat
      241 ctccactgtc tccgtgggag tagtggcaat ggtatcccgc tttgtgggag caaaagataa
      301 agaaagtgcc acgtacgttt cccgacaggc ttatcttttc tccatcttca gctctttgac
      361 cttaatgata actggaattg ctttaactcc ttatcttttt agtctgatgg gaactgaagc
      421 tcttgtgacc caagccggaa taaggtattt aagaataatc tttttgggtt cgatcttctt
      481 cttcctgatt gatgttttca gctcgatttt cagggcttcg ggtgatacca aaagaccctt
      541 atgggtgacg cttttcagtt tgggcttcaa tatagttttg gaccctttct tgatatttgg
      601 ctggggaccg tttcctcgtt tggagaccag cggagcggcc atagctacgg ttatatccca
      661 atgcttaggg gttttaatct tcttctattt aatcaaaaag gggaagctct tatttaagtt
      721 ttctctaaag ccggcactta aggtcgattt ctcaatcatt cgccgaattg tgagaatcgg
      781 catccctccc tccatcgctg gaatagtctt cagcatagtc tatctcttcc tcaatagaat
      841 agtggctcat tttggaactg aatccatcgc cgctctggga attggcaacc gtatggagtc
      901 cctttcttat ctcagttgtt tcggcttctc cattgcggca gctactttgg tgggacagaa
      961 cttgggtgca ggcaaaccgg aaagggctaa taaatcggcc tggagaacga tttacatcac
     1021 cacttttatc actggcttca tcagcgtctt atttttgtct ttccccaaag ccatctcctc
     1081 attttttatc actgacccca aggtggtgcc tatcgccatc aactatctta ggatattgtc
     1141 tatctctcaa atctttatgg cttgggagat agtgctggaa ggtgcttttt ccggagcagg
     1201 agatactctg cccccaatga tagtctccct gcccggctcg atactgcgca ttcccatcgc
     1261 ttatctttta gctataactt tgggcctggg agtaaatgga gtttggtggg ctataaccct
     1321 aaccagtgta gttaagggga cagtcttggt tttctggttt aggcagggaa aatggaaaaa
     1381 gaggcagatt taaaggcaca ggggcttgac agccagagga aatttgcttt attgaaatca
     1441 taaatttcct ttttttctgc gagagaaagg aagggtggct gtgctaagca acatagaaag
     1501 aatgactctg aaaaacaaga tggttgtctt gctaaaaccg gatagctcct ctcctttggc
     1561 aaccataaat cttagaatca aatccgggtt cttaaacgag ccggatgagc ttactggcat
     1621 atctcatctg cttgagcata tgctcttcaa gagcacaaag aagatggaga cagcccaact
     1681 caaagaaaag ttcaggcatt taggtggagt cttcaatggt gagactatgt atgattacac
     1741 catgtactac tgcacagtgc ccaaaaataa tctcagggct gcgcttgaac tcgaggcgga
     1801 tttggtctat aattcacttt ttgaccgagg tgaattagaa aaagaaaagc aggtggtcat
     1861 tcaggaaata agaagaaaag atgatatgct ttcgattttt gcttgggaaa agatgatgga
     1921 gaactctttt gtgaggcacc cttttagaag atggagaatt ggaacaacgg aaaacataac
     1981 tgagatgaag agggaagata tcttaagatt tcataatgaa aactattccc cccgcaacac
     2041 cattctgacc ataaccggtg atttcgaaag cgacaaggtg agccattggg ttagagaata
     2101 ctttgaaaaa ctgagacccc tcccccccaa gaaactttcc tttccaattg agccggagca
     2161 aaccagcttg agataccaaa gcctgaaggg ggacttgaaa caaacttatc ttaagattgg
     2221 cttccatgct cctggagtat tggaggagga taattttgct ctggttatcc tatcgtatat
     2281 tttaggtcaa ggcaagagct cgaggctaag ccaaatactt aaagccagac aagggctggt
     2341 taattcgatt tcctctgatt tatataactt gaaagattta ggggtttatt tctttgaagt
     2401 tgaattggaa gataagaata ttctacccgt cttaattgag gtcttcagac aaatagagaa
     2461 aatcaagcag agcaagcttt ctaaggcgga acttgcccga gccaaaaacc tctggcgcgc
     2521 ggatttctta tccaattttg agagtttagc tggtcaagct gatatcctct cgctttttga
     2581 gcaatacggg gattatagat taattcaaaa acttatgcac aagatagagc aagtgagtaa
     2641 gaaagatatt cagaaagcgg ctaagagata tttgaatttg gataaagcta ctgttttgag
     2701 atatgctccc caaaaagata aaacagctct tgaaagcttg aggagaaaaa taattttggg
     2761 gattaaagct aaagggcctg agttcaaacc ggtcaagtct atatccttta agaaaattgg
     2821 atctgcacaa aaagagggaa agtcgcttaa cactgaattg ctcaattatg gcacctatct
     2881 gctaaccagg gagaagcata ccctgcctat ggttactgtt gatttctatt tcaagggagg
     2941 gagaaacgac gaaaacgagg gcaattgtgg aataactcga ctatgtttgg aaaacgtcct
     3001 caaaaccaaa tccaaaggtg aaagcctaac cagtcagctc gaagtcctgg gcgggacttt
     3061 aagcacaacc gctgagccag acttcttcgg atactccatg gagatatcgg ctgaaaactt
     3121 ggatggtgct ttaggcatag tttttgaaat cttacagcat ccgacctttg aaaagaagat
     3181 agcggatata gaaagaaaac tccttatcgc agagattgaa aaatcacagg atgatatgct
     3241 gaattaccct gttcaccttc tgaaaagaac cattttcccc ggacatccgt atgccaaaag
     3301 cctttgggga gatgaaagga gtctcgagaa aataaccctc aagcaacttg aaaattggca
     3361 caaaacctat tatattaggc aaaacttggt aatctccgca gtgggagatt ttaaaaccga
     3421 atggttaaag aagaaaatag aattgggtct gaaaagttta ggcagagtta aaattgaaga
     3481 acaaaaaatg atactagtac atcaaaccaa agagagaaca aatattgttc cacgaagcaa
     3541 gaaccagact gccatagctt gcggatttct aacctgtggc tttgcggatg atgacctcta
     3601 tccattaaag ttaatccagg gcatattctc cggaatggga ggaaggttat ttacggagct
     3661 cagggaaaaa aggggaatgg cttatgcggt ttatgctgcc tgcgacgcct ataaaaatgg
     3721 gggcatcttt tacggttaca taggcacctc tcctcaaaag gaaaaaatag ttattgattt
     3781 gctcaaacag gagtttgaaa aaattggaaa gcgtaaagtt gaagcggatg agctggaaaa
     3841 atcaaaagcc tacatcagtg gagcttacaa tatatccctg cagaccaatt ccgccttagc
     3901 tcatcaatat gctaagaatc atattttggg aagagatgcg caccaggcag aggagtttgg
     3961 tcaaaggata gataaaataa cttcacggga aattttgcac atagctcgaa agtatttcga
     4021 tttgaacaat ttttacttag gtatgataag gggaagaaaa tgatgtcaac cggtttggtt
     4081 tatcatccag attaccttct gcacgacact ggtgcgggcc atcctgaaaa taaagaaagg
     4141 ttaatatcct taatgcaaca cttagagaaa acaggggctc tttctcaatt gaaattgata
     4201 cagcccaact ctaacttggc tctcgaatgg gtgaagaagg ttcactctcc agactacatt
     4261 gagctggtga agcaaacttg cgagaaggga aatgggatgt tggacccaga tactgtggtt
     4321 tctaaggatt ccttcaatgt ggcccttctg gcagtggaag gggtgctcca gggagtggag
     4381 gaagtgatgg gaagtaacct gaagaatgtc ttctgtgcaa ttcgcccccc aggtcatcat
     4441 gctgaaaagg aaagagggat gggattctgc attttcaata atttggctat tgcggctcag
     4501 tacctgctcg aaaaacacaa gttaaataga gtgttgattg tggactggga tgcccatcac
     4561 ggcaatggaa cacaagagat attctgctct gacccccgag tatattattt cagcatacat
     4621 caatttccct actatccggg aactggaaag gaaaatgagg aaggcgagaa caaaggaaag
     4681 ggattcacct tcaatatgcc tatggcagcc ggaagtggcg atttggaata tgtcgaagct
     4741 tttgagaaca ttctttcccc cctggctctg aaattctcac cggaattcat tctcatctca
     4801 gccgggttcg atgctcatca agatgaccct ttaaccggac ttaaggtcag cactaaaggg
     4861 ttcaaaaaga tgacgcaggt gacaaagaaa ctagcagata gttgctgtca gggaagaata
     4921 gtctcggcat tagaaggagg atatcatttg aaatccctgt gcgactcagt ggagactcat
     4981 cttagggttc tgatggaaga ttagatttat gaaaaggttc gctcgcttta aaatagagga
     5041 taaagagcat tacgggatag tagaggaaaa aatatatccc cttgagggaa atatattttg
     5101 ccagtatgag gttagctcat acggttacaa cttggatgag gtaaagcttc ttcccccctg
     5161 tcagccaacc aagataatcc ttgtaggtct gaattaccac gatcacataa aggaaagctc
     5221 ctctgccagc caagctccag aagagccggt tatcttcatg aaaccaacca gtagcctaat
     5281 cgccacggag gagaaaataa tttatcctcc cggggtcaag agattagact tcgaagctga
     5341 gttagctgta gtcatgaagg agagatgcaa agatacctct gcagagcaag ctggtaacca
     5401 tatcttcgga tatacctgct tgaatgacat aactgccaga gacttgcaaa caaaggatat
     5461 tcaatggacc agggctaaat ccttcgatac tttctgcccg ttgggtccct ttatctcggt
     5521 tggcatcgac ccaagcaacc tgaaaataga atcgtacctt aacggaaaag tgcaacaatc
     5581 ctccaatacc aatcagatga tattctcagt ggaggagatc gtaagttttg tctctaaagt
     5641 gatgacttta ttgcctggag atgttatctc cactggaacc ccatctgggg tcaattccct
     5701 gattcccgaa gataccatag agataagaat agagcatata ggcattttga gaaatcacat
     5761 catgccagcc taactatcct ttcaaaaata tactgcttaa attcaacttt tccatcttcc
     5821 cgccgataaa acaaatgatg taaccaatta accagggatg gtatcttaaa catatgagtg
     5881 ctatgtccaa agctcccaat atattggtgg tggatgatga aaaatatatc tgcgatatca
     5941 taaaggagag tctctccgat ttcgattaca atgtgctggt aactgatagt ctacagaaag
     6001 cgattcaaat aatcgaaagc caacccatag atttcctctt aactgacctg atcttaggtg
     6061 ataaatcggg tatggaggta ttgaacaaag ccatagagct acacccggat gtagtggttg
     6121 tcttgatgac tggccagccc accatcgaga atgcggtctc agtactgaag ctgggagctt
     6181 atgattatct gattaaacct ttctccgtgg aaagactaaa agccattatc cgacgtggat
     6241 tagaaagaca acagctttat cgggagaacg ttcatcttaa ggagatggtt tccctatatc
     6301 agataagcca agctatgggc tcaaccgtgg aattggattc tttattgaat ttgatattag
     6361 atacggcggt gcgtgaattc ggagccaatc tgggctcaat tctcctctgg gatgaaaaag
     6421 ctaagagctt aaagccaaag gcgtctctgg gcacaactaa agggaaatcg aatcagagag
     6481 atttgagcga ggaggaccca atcatcgact ccatcataaa aaacggtcag cctcagattt
     6541 tggatgaaag taacatacgc caaatgctat ccagcgaaac caaggggaga gatattaagt
     6601 cagctatcag ccatcctctt ctggctaagg gaaaggtaat tggggtattg aatttgattc
     6661 gcagccaaag agccaacccc ttcaccaaag gacagcttca atccctttcc attattgcca
     6721 gcgaagccgc ctctgctatt gagaattcaa aactgtatga aaacctcaaa ggatcttatt
     6781 tggagaccct gactgccctg gccaatgcag tggaggcgag ggatacatac acccgcggcc
     6841 acacggagag ggtatggtac atagcggagt tcttggccgg ccagatgggc tgggaagagg
     6901 agagaatgcg ggaggtcaaa atgggtggaa tactgcatga tattggcaaa attggagtgc
     6961 cggatgctat cttgaataaa cctggaaaat taactctaga ggaatttgaa atcatgaaac
     7021 ttcatcctca acagggggtc aaaatcttag aaggaatctc ctttctggct ccggctttgc
     7081 cttacgttct gtatcatcac gaaaggtatg atggccaggg ttatccggaa gggttagcta
     7141 aagaagatat tcccgttcaa ggaagattga tggcggtggt ggatactttc gacgccatat
     7201 ccagtgacag gccctaccgc aaaagcaaag ggttcgaaaa agcgttagag gaaataaagg
     7261 agaatgctgg cacccaattc gacctctcca ttgtggaggt gctgcttgat gcctggaaca
     7321 agggtctgat tgataaagga aagctcgaag ttcagacttc caaagaatca ttcgcttctt
     7381 cggctaaagt gaaaagctaa aagattcaat cacagatttc tgttgacacc tattcaaaag
     7441 aaactatatt caatgttaaa ttgaaattgc cagttgagaa gactgcaatt tttccctcaa
     7501 gataccttta aatttacttt ttatttagag catagattaa atttttgtaa gttttctttt
     7561 aaaaggagta aaatatgaaa aagataaaag aaaaaatcga tttcgatatt aatcagcttt
     7621 ctaccccctt tccccgctta ggcgagttgc gcttccctct gctgggccaa atagttttag
     7681 tgacttcaat cagtcgagag ggaatcccgg acgttgcgcc caaaagttgg atttccatct
     7741 tctcctctaa cccgcccata gttggattcg gctgtaacac taatcatacc accgccaaga
     7801 acatcctctc cactggagaa tttgtgatca atatcccggg ggaggaaata gtgcaaagtg
     7861 cgtggaaaat catagaattg gacaaagatg accccaaaaa gatcgagaag gcagaattaa
     7921 ctctaaccaa gtcaaccaag atatctacgc ctcgcatagc tgagtgcaaa gcgcatctgg
     7981 agtgctcctt ggattggact aagaaatata aagaggagat agtaattttc ggaaaggtac
     8041 ttttggcggc aatggacaaa gaggcagtgg agaaggatgt ggacgaaaga tacaaatact
     8101 tcaaactttt ggcttatttg gatgaaagga cttacggggt tataaatgct gctaagaagg
     8161 tataatcttg tacaaacaga tatcggccaa caagagaaat agcttctttc tggttttatt
     8221 cttcattgtc ttcatcttat gcttgggatg ggtcttcggt caggcccagg gctggggata
     8281 cgctgggctg gtggttgctt ttgttattgc cctagttctg gctctgattg gttattttca
     8341 aagcgacaaa atcgttctgg cagtgagtaa agccaagccg gtggatgaaa aggagaatcc
     8401 ctatttagcc aatgtggttg aggggttagc cattgccgca gggataccta aacccaaagc
     8461 ttatattatt gaagacagcg cacccaatgc ctttgccgct ggaagaaatc ctaaagttgc
     8521 cgcaattgtg gtcaccactg ggctcctgga caaactcgac cggctggagc tggaaggggt
     8581 tatcgcccat gagatgtcgc acatcaagaa ctacgatatt cgctatgcca ccttggtggt
     8641 tatcttcgtt ggcacaattg tcctcatttc ggattggatg ggaagaagtt ttttttatgg
     8701 cagaaggaga agggactcca gaggtggtca aattgaaggg gtaatcatac tggtcgcttt
     8761 agcattggca atcctctctc cactcattgc caagttagtg cagttagcta tttcccggca
     8821 gagagaatac ctggcggatg ccaatggcgc tctcttgacc aggtatccag aggggctggc
     8881 ctctgctctg gagaaaatcg ccaaagacac agagcccctg gaagtggcaa ataaagccac
     8941 tgcccatttg tatatagtca accctcttct cgaccataag ggtaaactga atgaactttt
     9001 ctcaactcac cctcccacag aggaaaggat taaaagactg aggtttatgt aagaatggag
     9061 aagagatgag acaaaaagtt ttcatttttg aagatgccgc atatgctaat ttccttcctt
     9121 tatcttacag ccgcccggtt tacaaattac ttttcggaat aaacacctta gagcaaaaga
     9181 taagcctcct tttcccggag gctgaaatca ctcttttgtg tagagattat ctcaaagatt
     9241 ggattggcac aaaaacccaa ttgaaagtca attcctttgg ggtagaggaa gccgaccaga
     9301 tacttttgat taatggtcga gcgatatttg atgaaagctt gagatccaat ttatcttttt
     9361 cagatgaaga taaggtgtat ctctgtgcca aggaggttgt ggcaatatcc cttaagggaa
     9421 gctctttcaa gaagtacaaa gagaaattgg tccaattata cgagccacaa aatctagacc
     9481 atatcaaagg gcaactgaaa aatgtcagaa ttaaagctga gatggcaagt tatttatgga
     9541 atttagttga acgaaatggc gaacagatta agaaagattt tgacctattt aagaaaaact
     9601 caaaattcaa aactaacagg aaagcgagtc aaattgattc ccaggcagtt ctctataacg
     9661 ataaggagat attcatcggc gaacaatctc aaatcgatgc ttttgcggta ctggatgcaa
     9721 gaaaggggcc gatttatatt gaccgaaaag taactatctt gtctcatacc cgaattgagg
     9781 ggccctgcta catcggagag gaaagccaga ttctgggagg agcgataaga gagggaaatt
     9841 cttttggacc ggtaaatcga atagctggag aagtggagaa atcgattatt ttgggataca
     9901 gcaataaatg ccatcacgga tttttaggac attcctatgc gggagaatgg gtgaatttgg
     9961 gtgcccttac caccaatagc gacttaaaga acaattacag ttcgattagg gttatgttaa
    10021 atggggagat ggttaacact ggcttaagca aggtgggtgt ttttctggct gaccatgtta
    10081 agaccggaat aggaacgctc cttaacagtg gaacaactgt tggctactcc actaatatct
    10141 ttggcggagg aatgcccaag gagaaattca ttccttcgtt ttattgggga ggaaaagatg
    10201 gatgggtaaa gtatcagttg gagaaagcaa ttcaaactgc cgaaataaca gttatgaggc
    10261 gtcaagttaa agctggcgag aatttgaagg agcttttcca taagatattt gagttaactg
    10321 agaaagagaa ggaactttga gtctattttg aagttgacaa aacaagagcg tttttgtact
    10381 gtttaggcga gccccaaagg agataagaaa atgcctcaac tgagaaaaga cccggtcatc
    10441 ggaagatggg tcataatctc aactgaaaga gggaaaaggc catctgattt tcccgctcct
    10501 cccaaaaggc aggaaagtaa gctttgcccc ttttgtccgg gaaatgaatc ttccactcct
    10561 cccgaggttt tggctttcag gcctgaccaa tctccagcca acaagcccgg ctggacctta
    10621 agagtcatac ccaataagta tcccgccttg aaagttgagg gtgagcttaa ccgtgaaggc
    10681 cgtggcatct tcgacaaaat gaatggagtg ggagcgcacg aggtgataat tgagactcca
    10741 gaacatacca aggagttagc tgacctcgac caaaagagag tggaggatgt gctctgggct
    10801 tatcgtttaa gaaccgtaga cctgaaaaat gacccgcgct taagatatat tctcatcttt
    10861 aaaaatcagg gattggaggc cggggcttct ttggagcata gccacagcca actcatcgct
    10921 acgcctatca tacctaaaag ggtgagtgag gagctagacg gctctaagaa gtattacgac
    10981 tacaaggaac gttgtatctt ctgtgacata gtcaaacaag aacaagagga gaatgtcagg
    11041 gtagtgaatc agaatgaaag tttcatttcc attgagccgt ttgctgccag atttcctttt
    11101 gagacctgga ttttgcccaa aaagcattat tctacctatg atgacaccaa acagcaagag
    11161 atgccggcat tggccgcgat actcaaagac actctaagta gattagccaa agctctaaac
    11221 aacccacctt acaattatac tatgcatacc cgaccagtag gtgatagttt taccaaggaa
    11281 taccactggc atattgagat aatgcccaag ttgaccaaag ttgcaggatt cgaatgggga
    11341 agcgggtttt acatcaatcc cactgctcca gaggatgcct gtaattactt gcgttctata
    11401 caactttaaa ttgaacggag tgaaatccat aggaaggggt gagcttgcta aaagagaaac
    11461 ttaaaatcgc tatagtctct tccgaggtag tcccctttgc caagaccggt ggattggcgg
    11521 atgtggctgg ggctctgcct aaggtcttat ctaaattagg acatgaagtt aaggtttttc
    11581 tgccaaaata caaaatggtg gatgaccaga aattcaatct aattgataag aaaatagaat
    11641 taagtaagat accaattggg gaaaaacaga ttgaattggg actcagatct gcaatgcttg
    11701 ccgattctaa agcagagttt tacttcttgg tgaacaattt ctattatctt cgggatgagt
    11761 tttatagaga caatagtact ggtctggatt atatagacaa cgacgaaagg ttcatcttcc
    11821 ttgctcgtgg aaccttagag gttttgaaga aactggattg gcagccggat attatacatt
    11881 gcaatgactg gcaatccggg cttatcccgg cttatttgaa gactctatac aaagatgacc
    11941 cgttttttaa gaataccgca accctcttct ccattcataa tttggcttat cagggaaact
    12001 tcccttccga gactttcgac aaacttggtt tagaaagaag cctcttctat cctgcttccc
    12061 cttttgagtt ctggtcaaag gtaagcttca taaaagcagg catagtttat actgatattg
    12121 taaatacggt aagtccaact tatgctctgg aaatacaatc tggttcggaa tatggatatg
    12181 gaatggaagg ggtcctgtcc agccgtaagg atgacttgtt tggcattatc aacggcattg
    12241 actacgatat atggaatcca aaaacagatg agcttatacc ctataaatat gaccctgacg
    12301 acctatctgg aaaggaaaaa aataaagaat accttttgaa aaccagcaag cttcccttgg
    12361 ataagaaaag aactccgctt gtcggcgtga tttcccgtct ggctgaccaa aaaggctttg
    12421 acctgattgc tcagatttct gagagacttt tgtccttgga tttaaagatt gtgattttgg
    12481 gaaccggaga tgagaagtat catttgcttt ttactaatat gcaaaagaag ttccctcaca
    12541 aactaaaggt cactttggga tttgataaca aactggctca tttgattgaa gcgggaagcg
    12601 atatgttttt gatgccttcc agatatgagc cttgtggctt aaatcagctt tatagtttaa
    12661 aatatgggac tgtgcccatt gtcagacata ccggcggctt ggcagataca attgccaatt
    12721 acaatcccat tactggcaaa ggaaatggct tttcttttaa aacttacagt gcggaaaagc
    12781 ttttggaaac aattcaaaag gcaatcaatt tgtataaaag tgaaactctc tggaccaagt
    12841 tgaccttagc tggaatgaaa caggatttct cctggaaagc ttctgccaag aaatatgtgg
    12901 agttgtatca aagggcgttg agtaagaagt ggtaagagaa agggatttgt agcttttgta
    12961 gggacagaac agtgttctgt ccctacgtta ttatgcaggg gcttgattta ttgaaaatcc
    13021 cccgccattg gcggggcgat caagcccgcg ggtcggatat ggttcaacaa gttcaccatc
    13081 ctgagcaagg tcgaaggaaa tccgacccct acataacaaa accgtaggcg acattccgga
    13141 atgtcgcctg caaaacctaa tcgaaaattc tcctgtcaag gttgggaaag tcaataaata
    13201 aggaccaata tttctcaaga attagttttc ttattttgac tggggcaaat ccaatttccc
    13261 ttaaaaaaca tagttctatt aatattttta tcttttgatt gagattatgt agctctttac
    13321 cctttgcgga ttttcccttc agcgctttat cgaggtgaac taaataattt ctcgtaacaa
    13381 caactttctg aataaaggat tttttatcgc caataaaaca ttttgaaatc ttattgaatc
    13441 tacgtagaat atctttaatt ctcccacgaa aactcaattc attgctataa ttaagtttct
    13501 gctccagcca ttttttatga ggacatggag ttgacttaat gatttcatta attctcttgg
    13561 catgtttctg aattgataat tcgaaattat tcatggttct tctgtgatat gattcaatag
    13621 caagtgccag gttcagaaaa atttgttctt tatgtaaaaa aggctcatat aattgggtca
    13681 ggtatagatc ataaacgggt tttagcaaat cttctttatt taaccaattt tctaggaaaa
    13741 tatgttgttt gctattcata tctttaaacg taaaaagcat cctatttggt atcggttcct
    13801 cagatgcaaa acgaataatt ttcggggaac aaataatgtt gatcgaataa tagacttgct
    13861 ccccattttt cgatacttct gaccttgtat tcgtaatacc tgcaatacct gatgggtgcg
    13921 tcccactcga aactgcaaaa gaaaggaagc tttgaatcgc atatataata tccaaatact
    13981 cttcaaaata gatgcctttt ggtgctctta tccctatata agcattctct tcaatgttta
    14041 tctttttcgg ctcgcgtata agtaaaggat taagacccaa aaatatcttg aaatctttaa
    14101 aatcagccat ttggattgac tttagtcttt catattttgc aatttcccct ttcttatgtg
    14161 gcaaaaatct tgtctcaagc gaggaaattc tagcccattc gtataaatac aaataataaa
    14221 taaataattg attgaattta atatcctctt ttctctcaaa atgaactcca ataaaaacga
    14281 tatctacttc gaaggtggct ttttctaaac tatgaagact ggttagatgt tcggcatggg
    14341 aacatttata aagagttatt aactgtccat ctggggaagt tcccaaaatc aaatcgtgcg
    14401 agatctcggt aaagatcttt tctttatttt ccgaggaata agttagttct aaagtgattt
    14461 ctttgccttg atataattta atgattccag gaactctcat ttctggtttc tctggaagcc
    14521 accacatact actatattca aactgccaag tcattttgtt ctttcttgtt tggaggttat
    14581 gtcaggagtt actcctgaca tttcaaacaa ccctattcca acaccctctt cctctcttct
    14641 cttatcactt tataaagatt tttcgcctct tcttttgtta gatttgtctc cgggggaacg
    14701 gaaaggattt ccgcttccaa caatctatta gaaaaatcaa tttggatttt ttggccagct
    14761 cttacgttgt ggctggcttt ggcaggtatc ccatc
//
LOCUS       DASWLN010000188         7275 bp    DNA     linear   ENV 20-FEB-2024
DEFINITION  MAG TPA_asm: Candidatus Zixiibacteriota bacterium isolate
            SMAG_U2949 k141_1143454, whole genome shotgun sequence.
ACCESSION   DASWLN010000188 DASWLN010000000
VERSION     DASWLN010000188.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37960594
            Sequence Read Archive: SRR10376091
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Candidatus Zixiibacteriota bacterium (soil metagenome)
  ORGANISM  Candidatus Zixiibacteriota bacterium
            Bacteria.
REFERENCE   1  (bases 1 to 7275)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 7275)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (26-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/12/2024 08:28:26
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 1,440
            CDSs (total)                      :: 1,416
            Genes (coding)                    :: 1,412
            CDSs (with protein)               :: 1,412
            Genes (RNA)                       :: 24
            rRNAs                             :: 1 (16S)
            partial rRNAs                     :: 1 (16S)
            tRNAs                             :: 21
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 4
            CDSs (without protein)            :: 4
            Pseudo Genes (ambiguous residues) :: 0 of 4
            Pseudo Genes (frameshifted)       :: 0 of 4
            Pseudo Genes (incomplete)         :: 3 of 4
            Pseudo Genes (internal stop)      :: 1 of 4
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..7275
                     /organism="Candidatus Zixiibacteriota bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_1143454"
                     /isolate="SMAG_U2949"
                     /isolation_source="soil"
                     /db_xref="taxon:2053527"
                     /environmental_sample
                     /country="USA: Oregon"
                     /lat_lon="42.99 N 123.63 W"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            complement(229..1269)
                     /locus_tag="VGB16_06975"
     CDS             complement(229..1269)
                     /locus_tag="VGB16_06975"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_013899124.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="AIR synthase family protein"
                     /protein_id="HEX9917466.1"
                     /translation="MAKVGKIDLEVFTKFVLSHLGAENKKVLVPPLTGVDACVIDIGN
                     DKVLITAEDPIFPAPGLPLETFGWFTVHIGASDVAVMGVKPEFMTYSLLMPLQTEDKD
                     LEIMVDSIHRAAKDLDIAIVGGHTGYYPAVTLPIIGGISVFAIARKDEYVTPAGAKVG
                     DRIILTKGPAIEAAGLLSVIYEKKLLYHYPKELVDKAKGLCNSITVVKDCLVAKQVGG
                     VTAMHDATEGGVIGGLFEVANASDVGMEVDESLFIYPPEVDMICDYLKIDALEAIGEG
                     SLLICVSPERANMVVSELKKNNIDSSIIGRVTDKRSGRFIKRIDGSKRELEIPKQDPF
                     WPAFFKGLGEIG"
     gene            complement(1282..3300)
                     /locus_tag="VGB16_06980"
     CDS             complement(1282..3300)
                     /locus_tag="VGB16_06980"
                     /inference="COORDINATES: protein motif:HMM:NF024583.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_process: GO:0000160 - phosphorelay signal transduction
                     system [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="response regulator"
                     /protein_id="HEX9917467.1"
                     /translation="MTSLLIEVIFSFTLFLLLIFLYSIRERYLVLTFKGWDKLLLGIF
                     VLLLGSLFDLTDNFASVQRVFLLDNLPLNLVLKIGFYIVGMILVVSGPLNWLSEILEW
                     KRKTKEKDRIVNLLVGFSAKGRGYKSLLDMFNFVLVEVGHYFQVEFGAIFLLDDLKEF
                     LILESHVGLSSNAIHNLERIRLNDSAFSRVVKDGTTQLINDIFFEEKRLALHLKEDNL
                     ESLLAAPIVAKGKISGVLTVFSKERFKFNQEDAKLFLSLGEQMGEIVENMRLSGELMR
                     KDERLKKVESQAKLISKITSALASVYQTDKVLDRLTLDSLQMIEASVAMLFSIEGEDL
                     TLRSSLNEDSIGKKMRISDFPLIGEVIEKKKPMVKTELFRGSEPESIVSKRRVKSSLA
                     VPLLSEDKVLGVLLFENHEHSRTYSAWEIEQVQTLANLATLALEKDKTNRELQAILGD
                     KRLGASLGEWSNDVNNMLAGILGNIQLLQENIKDKRVVSAHQTTEYLKTVEEAVLATS
                     RMVKKIQNVPVTEKEEITPKRKITIPREELVKPEVKKEKEIYKVLVVDDQEIIRDLVQ
                     NILKGLGYQSVLASNGREGLEKFREERFDLVITDLGMPDISGWEVASDIKKQREDIPV
                     ILITGWGIHPDPNKMKDSGINFLLTKPFKVDQLEKVIQDAVELIRIKK"
     gene            complement(3412..4713)
                     /gene="miaB"
                     /locus_tag="VGB16_06985"
     CDS             complement(3412..4713)
                     /gene="miaB"
                     /locus_tag="VGB16_06985"
                     /EC_number="2.8.4.3"
                     /inference="COORDINATES: protein motif:HMM:TIGR01574.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016782 - transferase activity,
                     transferring sulphur-containing groups [Evidence IEA];
                     GO_function: GO:0051539 - 4 iron, 4 sulfur cluster binding
                     [Evidence IEA];
                     GO_function: GO:1904047 - S-adenosyl-L-methionine binding
                     [Evidence IEA];
                     GO_process: GO:0006400 - tRNA modification [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="tRNA (N6-isopentenyl
                     adenosine(37)-C2)-methylthiotransferase MiaB"
                     /protein_id="HEX9917468.1"
                     /translation="MKKNKSAYFLTFGCQMNVYDSDVLKSILTKNNHSIADGLENADL
                     IVVNTCSVRHRAEVRALGRIRDLKQYKLSNPNLKIAVVGCMAQRMGEDLLKELPFVDL
                     VLGTERLFELDTYLEKLEGNRIVDTRVDGKLPDGTIPKSDSDYSAFVAISRGCNNFCS
                     YCIVPYVRGQEKHRPAKQIIDEVRQLAQKGYKEVTLLGQNVNSYKDGIYDFADLLQMI
                     NDNTEINWIRFMTSHPKDLSSKLIDKISNLSKVCEHLHLPLQSGSDIILQRMNRNYTS
                     SDYLNLVQQIRGKVGDLCLTTDIIVGFPGETPSDFQKTLSVLEKIRFDAAFTFRYSPR
                     EGTKAESFEDDVPEKEKIRRLEILIVLQKEISQKQNQNFVGRELEVLIDGYSKKDKTR
                     LKGKTRTDKTVVLDKKEDFLGSFRQVKIISANPWTLFGQLQ"
     gene            complement(4819..5847)
                     /locus_tag="VGB16_06990"
     CDS             complement(4819..5847)
                     /locus_tag="VGB16_06990"
                     /inference="COORDINATES: protein motif:HMM:NF025574.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF4325 domain-containing protein"
                     /protein_id="HEX9917469.1"
                     /translation="MDLKALILKRLERKGRINSDEIVKATGFSRAYVNRFFQELRDQG
                     KIVLLGKANRAHYVLASEGAVTDAKSKITDITLNLENKELYEDKVLDQIKKKTGIFMG
                     LRKEISDILDYVFSEMLNNAIEHSASKIIRVVIKKDENNISFAVVDRGIGIFNNIMRK
                     KKLGSALEAIQDLLKGKQTTMPKLHSGEGIFFTSKVADMLIIQGSNKKLIFNNLLDDV
                     FIEDIKPVNGTKVNFSIERNAKKKLSDIFRKYTDSSFEFSKTSVTVKLFRMDSKYISR
                     SEARRIVAGLDKFKKIILDFKGIKTIGQGFADEIFRVWGSGHPEITIEILNANDNVRF
                     MIKRTKTQ"
     gene            complement(6202..>7275)
                     /locus_tag="VGB16_06995"
     CDS             complement(6202..>7275)
                     /locus_tag="VGB16_06995"
                     /EC_number="2.8.4.-"
                     /inference="COORDINATES: protein motif:HMM:TIGR00089.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008172 - S-methyltransferase activity
                     [Evidence IEA];
                     GO_function: GO:0016782 - transferase activity,
                     transferring sulphur-containing groups [Evidence IEA];
                     GO_function: GO:0051539 - 4 iron, 4 sulfur cluster binding
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="MiaB/RimO family radical SAM
                     methylthiotransferase"
                     /protein_id="HEX9917470.1"
                     /translation="AKIVVVGCYAQTEEEVLKTLPGVSLVVKNENKEEILRKITRLFD
                     GDSLIENKIPLLNQIEVSGHTKHTRGLVKIQDGCNQACSYCIVPLARGPEKSRRLDSI
                     LSEIGNLDKNGFREVVLTGVHIGRYDFEGINLHQLLDEILKRTQIKRVRLSSIEPKEI
                     ADELIDLASNNDKICRHFHIPLQSGDDYILSRMNRNYTTGEYAELLDKIIKKIPDCLV
                     GADVMVGFPEELESNFQNSLDFVLSQPINYLHVFSYSDRKGTVASKLPDKIPPHVIKK
                     RNKLFRELGYKKWVNFIEKFIGKKLGVLIEKRRDKKTGKLTGLSDNYIRVLIDGEDRW
                     MNQLVEVEITRREGRVLVGEFVG"
BASE COUNT         1995 a         1754 c         1221 g         2305 t
ORIGIN      
        1 gtgtcattgc gaggagggcg cagcccgacg aagcaatctc ataactatca gataaacaaa
       61 gagattgcca cgctccctcc cttcgacttg gctcagggac ggtgagcaaa gtcgaaccgt
      121 acggtcgctc gcaatgacaa aaaggcgaaa gagagtgttt ttagacagcc tcgttaacgt
      181 gaggttgtct cgtaactcac aactcaaaac tcgaaactta aaactgcttt accctatctc
      241 ccccaacccc ttaaaaaacg caggccaaaa ggggtcttgt ttcggaattt ccaattccct
      301 ttttgagcca tcaattcttt ttatgaacct accgcttctt ttatctgtaa cccgaccgat
      361 gatagatgaa tctatattgt ttttctttaa ctcggacaca accatatttg ctctttcagg
      421 tgaaacacaa atcaaaagcg acccttcgcc aatggcttcg agcgcatcta ttttgagata
      481 atcgcaaatc atatcaactt ctggtggata aataaataac gattcatcca cttccattcc
      541 tacatcgctg gcattggcca cctcgaaaag cccaccaatc actccaccct cagtggcgtc
      601 atgcatagct gtaaccccac ccacctgctt ggcaaccaaa caatccttca ccacagtgat
      661 ggaattacat aatcccttag ctttatctac caactcttta ggatagtgat acaagagctt
      721 cttctcgtaa ataaccgaca atagcccagc cgcctcaatg gctggtcctt tggtaagtat
      781 tatcctatca ccaacctttg ctccggcagg agtaacgtat tcatccttcc tggcaatggc
      841 gaaaaccgaa atccctccaa ttatgggcaa cgttactgcc ggataatagc cggtatgtcc
      901 tcccacaata gcaatatcca gatccttggc agcacggtga atcgaatcaa ccatgatttc
      961 aaggtctttg tcttcggtct ggagcggcat tagaagagaa taggtcataa attccggctt
     1021 aacccccatc actgccacgt ccgaagcacc aatatgcaca gtgaaccatc caaaggtttc
     1081 caaaggcagg cctggggcag gaaagattgg gtcttcggct gtgattaaaa ctttatcgtt
     1141 gccaatatca atcacacatg catccacacc ggtcaagggc ggaaccagaa ctttcttgtt
     1201 ctccgctccc agatgcgaaa gcacgaattt tgtaaaaacc tccaagtcta tcttgcctac
     1261 tttagccact agtccccccc cttatttttt gatgcgaatt aactccactg catcctgtat
     1321 tactttctcc agttggtcaa ctttgaacgg cttggttaaa aggaaattta tgcccgaatc
     1381 tttcatctta ttcgggtcag gatgaatccc ccagccggtg ataagtatta cgggaatatc
     1441 ctccctttgt ttttttatgt cacttgccac ttcccagccg gaaatatccg gcatacctaa
     1501 atcggttata actaaatcga acctctcctc cctaaacttc tccagcccct ctctgccgtt
     1561 cgaagccaga actgattgat atcccaaccc cttcaggata ttctgcacca gatctcttat
     1621 gatctcctgg tcatctacaa ccaaaacctt gtaaatttct ttttcttttt tgacttcggg
     1681 ttttactaac tcttctcggg gaatggttat ttttcttttt ggtgtaatct cttctttttc
     1741 agttaccggc acattttgaa tcttcttaac catccgactg gtagccaaca ccgcctcttc
     1801 aactgttttc agatactcag ttgtctggtg ggcggaaacc actcgtttat ctttgatatt
     1861 ctcctgcaga agttgaatat ttcctaatat cccggctaac atattattga catcattgct
     1921 ccactctccc aaactcgcac ccagcctctt atcaccaagt atggcttgca attccctatt
     1981 tgtcttatct ttttccagag ccaatgtggc taaattagcc aatgtctgca cctgctctat
     2041 ttcccaagca ctgtaggtac gggaatgttc atgattttca aaaagcaaaa ctcctaagac
     2101 cttatcctcg gaaagcaaag gcactgccag ggaggatttc actcttcttt tagagacgat
     2161 tgattccggc tcactgccgc ggaaaagctc agttttgacc atcggttttt tcttctcaat
     2221 aacctcaccg attagaggaa aatccgatat tctcatcttt tttccaattg aatcctcatt
     2281 caaacttgac ctcagagtca aatcttctcc ctctattgaa aagagcatgg cgactgaagc
     2341 ctcaatcatc tgtaagctgt ccagggtcag tctgtccaag actttatctg tctgatagac
     2401 ggaagccagc gcgcttgtta ttttggaaat gagcttggct tggctttcga ctttctttaa
     2461 cctctcgtct tttctcatta actccccgct aagcctcata ttttcgacta tttctcccat
     2521 ctgctcaccc aaagacaaaa agagcttggc atcctcttga ttgaatttaa atctttcctt
     2581 gctgaaaacc gtcaaaactc ccgatatttt gcccttagct acaatcggag cagctaaaag
     2641 ggattctaaa ttatcctctt tcagatgaag agccaacctc ttctcttcaa agaagatatc
     2701 atttatcagt tgggttgtgc catctttaac cactcgactg aaagccgaat catttaatct
     2761 tattctctct aagttatgga tggcattgga agacaagccc acatgggact ccaagattaa
     2821 aaactctttc aaatcgtcta aaagaaatat cgccccgaat tcaacctgaa aataatggcc
     2881 aacttccacc agaacgaaat tgaacatatc cagaagtgat ttatatcctc ttcctttggc
     2941 agagaaacca accaaaaggt ttactattct atctttttct tttgtttttc ttttccactc
     3001 cagaatctcg gaaagccaat ttaaaggccc acttaccact aaaatcattc ctacaatgta
     3061 aaagccgatt ttcagcacaa ggttcaaggg aagattatcc aagaggaaaa ccctttgaac
     3121 gcttgcaaag ttgtctgtca agtcgaacaa gctccccaaa agaagcacaa agattcccaa
     3181 gagaagctta tcccatccct taaaagttaa gaccagatat ctttcgcgga tactataaag
     3241 gaagattaaa agtaaaaaaa gggtaaaaga gaagatgact tctatcaaca aggaggtcat
     3301 cagtttattc ctggatagat ttggacggtt tctatacagt ataaccaaac cgacctcaaa
     3361 atcaagcaat tatttccaaa attttgtggg gtaaataggg agaagtacaa actattgtag
     3421 ctgtccgaac aaagtccagg ggttggcgga gatgatttta acttgcctga aactgcctaa
     3481 aaaatcctct ttcttatcca aaactacggt tttgtcagtt ctggttttcc ctttgagtct
     3541 ggttttatct tttttgctat acccatctat taagacttcc agttccctgc ctacaaagtt
     3601 ttggttttgt ttttgggaaa tctctttttg taacactatt aaaatctcca gccgtctaat
     3661 cttctctttc tctggaacat catcctcgaa actttctgct ttggtcccct cacgaggaga
     3721 atagcgaaag gtaaaagccg catcgaatct gattttctca agcacactta acgtcttttg
     3781 gaaatcggaa ggagtctccc cggggaagcc aactattatg tctgtagtta aacacaaatc
     3841 ccctactttg cctctgattt gttgcacaag attcagataa tcactcgaag tgtaattgcg
     3901 attcattctc tggagaataa tatccgagcc agattgtaaa ggtaaatgta aatgctcaca
     3961 gaccttagac aagttcgaga ttttgtcaat taatttggag gataaatctt ttggatggga
     4021 agtcataaat cttatccaat ttatttctgt attatcattt atcatctgta ataaatcggc
     4081 gaaatcgtaa attccatcct tgtaggaatt gacgttctgc cccaataaag taacctcttt
     4141 gtatcctttt tgtgctaatt gtcggacttc gtctattatc tgttttgctg gtctgtgctt
     4201 ttcctgaccc cgcacatagg ggacaatgca atacgaacag aaattattgc aacctctgga
     4261 tatggctacg aaggcagaat aatcgctatc gcttttgggt atggttccgt caggcaactt
     4321 accatccacc ctagtatcga caattcgatt tccttctaat ttctcgagat aagtatctaa
     4381 ctcaaataat cgctctgttc ccaaaaccaa atcaacaaaa ggaagctctt ttagaagatc
     4441 ctcccccatt ctttgagcca tacacccaac cactgcaatc ttaagattcg ggttggataa
     4501 cttatactgt ttcaaatctc gaatacgacc caaagcccgc acttccgccc tgtgacgaac
     4561 tgaacaggta ttgaccacta tcaaatctgc attttccaga ccgtcagcaa ttgaatggtt
     4621 gttttttgtc aggatagatt tcaaaacatc cgaatcatag acattcatct gacaaccgaa
     4681 ggtgagaaaa tatgcggatt tgtttttttt cataaccttt tcttgtaggc gacattccgg
     4741 aatgtcgcct tcgcttttgc atcttaccct gcaccagcgt agttcggggt ttgcccaacc
     4801 gcaaaactca caattccgtc attgtgtctt tgttctcttt atcataaatc gaacattgtc
     4861 atttgcgtta agtatttcaa tggtgatttc cggatggccg gagccccata cgcggaatat
     4921 ctcatcggca aagccttgtc cgattgtttt aatcccctta aaatccaaga taatcttttt
     4981 gaatttatca agacctgcaa caattcttct tgcctccgag cgtgaaatgt acttggaatc
     5041 catcctgaag agtttaactg tcactgatgt cttgctgaac tcaaaagatg agtccgtata
     5101 ttttctaaaa atatcagata atttcttctt ggcatttctt tcaattgaaa aattgacttt
     5161 ggttccgtta accggtttta tatcttctat aaaaacatca tctaataggt tgttaaatat
     5221 caacttctta tttgacccct ggatgatgag catatctgcc actttggaag taaagaaaat
     5281 gccttctccg ctatgcagct taggcatagt tgtctgtttg cctttaagca ggtcctgtat
     5341 tgcctctaaa gcgcttccca actttttctt cctcattata ttattgaaaa ttcctatgcc
     5401 cctatcgacc acggcaaaac tgatattatt ttcatctttt tttatcacca cccgtataat
     5461 cttcgaagca gaatgttcga tggcgttgtt caacatttca gaaaacacat aatctaagat
     5521 atctgaaatt tcttttcgaa gccccatgaa tattcccgtt ttcttcttga tttggtcaag
     5581 aaccttgtct tcatagagtt ctttattctc tagattcaaa gtgatgtcgg ttattttact
     5641 ttttgcatct gttactgccc cctcagatgc aagaacgtaa tgagcccggt tggctttacc
     5701 aagcaggacg attttcccct ggtctcttag ctcctgaaag aatctgttga cataagccct
     5761 cgaaaaccct gtggctttta cgatctcgtc tgagtttatt ctccctttcc tttccagtct
     5821 ttttaggata agtgctttta gatccatata tcccgcctgt gtttagttta caactttatc
     5881 ctagaactaa agtttgaagt tgtcaacaag tcgtaaactt attcttaagg ttttaacgga
     5941 tttgtataca acttcaatat atatcagaaa ttataagttg tcaacaagtc taaaacctat
     6001 tatcagcatt catcagatag ccgtaggtga gggaatgggc agtttttttc tgtcataacc
     6061 ttttcttgta ggcgacccat gtggatcgcc tgcattccta tttcgttaga gttgtccaaa
     6121 cccgtcccga tttatcccgc ctctggcggg atctatcggg cttcgttggg cggatttatc
     6181 actatagaca aaacttcggg tctatccgac aaactcacct actaagaccc taccttccct
     6241 tcttgttatc tcaacctcca ccaactgatt catccaccta tcctctccat ctattaagac
     6301 tcttatataa ttatccgaca acccggtcaa tttacccgtt tttttatccc ttcttttctc
     6361 aatcaaaacc cctaatttct taccaatgaa tttttctatg aaatttaccc atttcttata
     6421 tcccaattcc cgaaaaagct tatttctctt ctttattacg tgtggaggaa tcttatcggg
     6481 tagctttgag gcaactgttc cctttctgtc cgaataactg aaaacgtgta gataatttat
     6541 gggttgggaa agcacaaaat ccaaactatt ttggaaatta ctttctaact cttccggaaa
     6601 tccaaccata acatccgcac caactaaaca atctggaatt ttctttatga ttttatccaa
     6661 aagctctgca tattcccctg tggtgtaatt cctattcatt cttgaaagta tataatcatc
     6721 cccgctttgc aaaggaatat gaaagtgcct gcaaatcttg tcattattac tagctagatc
     6781 aataagctca tcggctatct ctttaggctc aattgagctt aagcgtactc ttttgatttg
     6841 ggttcgcttc aatatctcgt ctagaagctg gtgcagattt ataccttcaa aatcatacct
     6901 tccaatatgc acaccagtca aaaccacttc cctgaaaccg ttcttatcga ggtttccgat
     6961 ttcagataaa atggaatcta atcttctact tttttctggg ccacgcgcca aaggaacaat
     7021 gcagtaactg caggcttgat tgcatccatc ctgaatctta accaatcctc gggtatgttt
     7081 cgtatgacct gagacttcga tttgatttaa aagaggtatc ttattttcaa ttagactatc
     7141 tccatcgaat aggcgggtta tttttcttaa gatttcctct ttgttttcat ttttcaccac
     7201 caaactaacg ccgggtaaag tttttaagac ctcttcttct gtttgagcat aacatcccac
     7261 caccacgatt ttggc
//
LOCUS       DASWLN010000189         2387 bp    DNA     linear   ENV 20-FEB-2024
DEFINITION  MAG TPA_asm: Candidatus Zixiibacteriota bacterium isolate
            SMAG_U2949 k141_879463, whole genome shotgun sequence.
ACCESSION   DASWLN010000189 DASWLN010000000
VERSION     DASWLN010000189.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37960594
            Sequence Read Archive: SRR10376091
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Candidatus Zixiibacteriota bacterium (soil metagenome)
  ORGANISM  Candidatus Zixiibacteriota bacterium
            Bacteria.
REFERENCE   1  (bases 1 to 2387)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 2387)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (26-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/12/2024 08:28:26
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 1,440
            CDSs (total)                      :: 1,416
            Genes (coding)                    :: 1,412
            CDSs (with protein)               :: 1,412
            Genes (RNA)                       :: 24
            rRNAs                             :: 1 (16S)
            partial rRNAs                     :: 1 (16S)
            tRNAs                             :: 21
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 4
            CDSs (without protein)            :: 4
            Pseudo Genes (ambiguous residues) :: 0 of 4
            Pseudo Genes (frameshifted)       :: 0 of 4
            Pseudo Genes (incomplete)         :: 3 of 4
            Pseudo Genes (internal stop)      :: 1 of 4
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2387
                     /organism="Candidatus Zixiibacteriota bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_879463"
                     /isolate="SMAG_U2949"
                     /isolation_source="soil"
                     /db_xref="taxon:2053527"
                     /environmental_sample
                     /country="USA: Oregon"
                     /lat_lon="42.99 N 123.63 W"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            45..821
                     /locus_tag="VGB16_07000"
     CDS             45..821
                     /locus_tag="VGB16_07000"
                     /EC_number="3.-.-.-"
                     /inference="COORDINATES: protein motif:HMM:NF013672.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016810 - hydrolase activity, acting on
                     carbon-nitrogen (but not peptide) bonds [Evidence IEA];
                     GO_process: GO:0005975 - carbohydrate metabolic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="polysaccharide deacetylase family protein"
                     /protein_id="HEX9917471.1"
                     /translation="MPDRPILLFHKIDNSFEWGITRQTIKQFEREIRFLSESGYKTVT
                     FEEIDNLSTQDKKVVLTFDDGYESIYQNALPILQKHNMFAYIFIPTGFVGKENSWDAN
                     LGNRRFKHLGWEQIKEMSEYGFLFGSHSVNHPDLTRLSDKHLKYELEVSKDQLEQELG
                     KKIDLLSYPFGKTNSRVRAMAKEAGYKKGFTICKSISPPNHFAVARKPLYLIDSLLSF
                     EIKLKQSNLVWIEDIKSKIINSFANGTILVKSLPRYEDVF"
     gene            1449..>2387
                     /locus_tag="VGB16_07005"
     CDS             1449..>2387
                     /locus_tag="VGB16_07005"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_014854820.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0000166 - nucleotide binding [Evidence
                     IEA];
                     GO_function: GO:0004812 - aminoacyl-tRNA ligase activity
                     [Evidence IEA];
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_process: GO:0043039 - tRNA aminoacylation [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="amino acid--tRNA ligase-related protein"
                     /protein_id="HEX9917472.1"
                     /translation="MQHYYIVDAGKYVGQEITIKGWLYNKRSSGKIHFLIIRDGTGII
                     QAVMSKGEVSEKVFNLYDQLTQESSLEVEGKIREDRRAPSGYELIISDIKIVQIAKDY
                     PITPKEHGTAFLMEHRHLWLRSSRQYAILKIRNEIIMAIRDYFYRNGFVLIDTPILTG
                     AIGETATTLFETKYFDLGKAYLAQTGQLYLEAACVSLGKVYCFGPTFRAEKSKTRRHL
                     TEFWMVEAEEPFYDNNDNMDLEEDFLCYIVKWVLKNSKAELESLQRAIPALENIEKPF
                     PRISYDQAVEILNKKGSSFQWGADFGGEDETLLSKEF"
BASE COUNT          768 a          457 c          522 g          640 t
ORIGIN      
        1 gaagaagata gactgggata aggtagggta atggatttat gtcaatgcca gataggccaa
       61 tattgctttt tcataagata gacaattcct ttgaatgggg tattaccaga cagacaataa
      121 aacagttcga aagagaaatc agatttcttt ctgaaagcgg atacaaaacc gttacttttg
      181 aagagataga taatctaagt acccaagaca agaaagtcgt cctgactttt gatgacgggt
      241 atgaaagcat ataccagaat gcccttccca tactgcaaaa acataatatg tttgcctaca
      301 tctttatccc cacaggattc gtaggaaaag agaatagttg ggatgccaat ttgggcaata
      361 gaagatttaa acatctcggc tgggaacaaa tcaaggaaat gtcagaatat ggatttctct
      421 ttggctctca ttcggtcaat catcctgatt taaccagact atcagataag catctaaaat
      481 atgaactaga agtatctaaa gaccagctag agcaagaact gggcaagaaa atcgaccttt
      541 tatcctatcc ttttggaaaa actaactcaa gagttcgtgc tatggcaaaa gaggcaggat
      601 acaaaaaggg ttttactatc tgcaagagca tatccccgcc aaaccacttt gccgtcgcta
      661 gaaaaccact atatctgatt gactcgcttt tgtcatttga gataaagctc aaacaaagca
      721 acttggtttg gattgaagac atcaaatcca agataataaa ttcttttgcc aacggcacca
      781 tactggtcaa atctttgccg agatatgagg atgtttttta gactgccatt cccgcaagag
      841 tgtaaattca gtccctaaag taaatggatt cctgctttcg caggaatgac ataaaacatg
      901 taggggcaat tcatgaattg cccctacgcc ctgttttggg ttgtgtcagg acatagtcat
      961 gacactctaa aacggcagga gtagctcctg ccataacgtt aaaggtttct aggaactgga
     1021 gtataaagcc cccgtagggg atctacgact ttagcttcat atcattttat atggcaaacc
     1081 tgaaggcttg cactccaaaa aacactggtt tgtttgtttg cggttggttc gggaattgtc
     1141 accaggatcc cgcttggcgg gagaattccc gaacctatta tttgtgcggg aaagccaatt
     1201 cctgcacaag tagagtaagt ttgtaccccg aaaacatcga ttgcttcctc ctgattaata
     1261 tcgggactcg catcatctat actgtcattg cgagaatccc gccaaaggcg ggagacgaag
     1321 caatcccaag aactcaaacc aaaattttta ttgccaatcc cctttatacc aaatatatta
     1381 gaaatcaata gatgatttag tgtagcagaa ctctttggtg ttttataagc tcacacatag
     1441 gggtaaagat gcagcattat tatattgttg atgcaggaaa atatgtaggt caagaaataa
     1501 ccatcaaggg atggctttac aacaagaggt caagtggcaa gatacatttt ctcataataa
     1561 gggacgggac cgggataatt caagcggtta tgtctaaagg ggaagttagt gagaaagtgt
     1621 ttaatctgta tgaccagctc actcaagaat cttcacttga agttgaaggc aaaatcagag
     1681 aggacaggcg agcaccgagt ggttatgagc tgattatctc tgatataaaa atagtccaga
     1741 ttgccaaaga ttatcccata accccgaaag agcacggcac cgcttttctg atggagcaca
     1801 ggcatctatg gttacgttca agcaggcaat acgctatctt gaaaatcaga aatgaaatca
     1861 taatggcgat tcgggattat ttttacagaa acggttttgt cttaatcgat acgcccatat
     1921 taaccggagc aataggggag accgccacca ctttatttga aaccaaatac tttgatttgg
     1981 gcaaggcata tcttgcccag accggtcagc tttatttgga agcggcttgc gtctccttag
     2041 gaaaggttta ttgtttcggt ccgacctttc gggcagaaaa gtcgaaaacc agaagacatc
     2101 taactgaatt ctggatggtg gaggcggagg aacctttcta tgataataat gacaatatgg
     2161 atttagaaga ggatttcttg tgttacatcg tgaaatgggt cctcaagaat agcaaagccg
     2221 aattggagtc tttgcaaaga gctatacctg ctctggaaaa catcgaaaaa ccatttccca
     2281 gaatttcgta tgaccaggca gtggaaatcc taaataagaa aggaagctca tttcagtggg
     2341 gagctgattt tggaggagag gatgagactc ttctgtctaa agaattt
//
LOCUS       DASWLN010000190        14308 bp    DNA     linear   ENV 20-FEB-2024
DEFINITION  MAG TPA_asm: Candidatus Zixiibacteriota bacterium isolate
            SMAG_U2949 k141_879714, whole genome shotgun sequence.
ACCESSION   DASWLN010000190 DASWLN010000000
VERSION     DASWLN010000190.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37960594
            Sequence Read Archive: SRR10376091
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Candidatus Zixiibacteriota bacterium (soil metagenome)
  ORGANISM  Candidatus Zixiibacteriota bacterium
            Bacteria.
REFERENCE   1  (bases 1 to 14308)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 14308)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (26-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/12/2024 08:28:26
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 1,440
            CDSs (total)                      :: 1,416
            Genes (coding)                    :: 1,412
            CDSs (with protein)               :: 1,412
            Genes (RNA)                       :: 24
            rRNAs                             :: 1 (16S)
            partial rRNAs                     :: 1 (16S)
            tRNAs                             :: 21
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 4
            CDSs (without protein)            :: 4
            Pseudo Genes (ambiguous residues) :: 0 of 4
            Pseudo Genes (frameshifted)       :: 0 of 4
            Pseudo Genes (incomplete)         :: 3 of 4
            Pseudo Genes (internal stop)      :: 1 of 4
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..14308
                     /organism="Candidatus Zixiibacteriota bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_879714"
                     /isolate="SMAG_U2949"
                     /isolation_source="soil"
                     /db_xref="taxon:2053527"
                     /environmental_sample
                     /country="USA: Oregon"
                     /lat_lon="42.99 N 123.63 W"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            complement(<1..913)
                     /locus_tag="VGB16_07010"
     CDS             complement(<1..913)
                     /locus_tag="VGB16_07010"
                     /EC_number="2.1.3.2"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_015757867.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004070 - aspartate carbamoyltransferase
                     activity [Evidence IEA];
                     GO_function: GO:0016597 - amino acid binding [Evidence
                     IEA];
                     GO_process: GO:0006207 - 'de novo' pyrimidine nucleobase
                     biosynthetic process [Evidence IEA];
                     GO_process: GO:0006520 - amino acid metabolic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="aspartate carbamoyltransferase catalytic
                     subunit"
                     /protein_id="HEX9917473.1"
                     /translation="MKLKSKHLIGLEKITRREIGLILDTAESFREVLERPIKKVPTLR
                     GITVVNLFYEPSTRTRISFELAEKRLSADTINFSTSTSSVKKGETLKDTVKNIEAMKV
                     DMVVVRHKSVGVPYFLTQCLDSSIINAGDGAHEHPTQGLLDLFTIRQKYKKIEGLRVL
                     FVGDILHSRVARSGIWGFKKMGASVAICGPTTLLPWEAEKMGVEVYSDLDKALQGRDI
                     VNVLRIQLERQQAGYLPSLREYATLFGITQQRLKNLNENFTIMHPGPINRGVEITPEV
                     ADGPYSVILDQVTNGVAIRMAVLYLLSG"
     gene            complement(930..1481)
                     /gene="pyrR"
                     /locus_tag="VGB16_07015"
     CDS             complement(930..1481)
                     /gene="pyrR"
                     /locus_tag="VGB16_07015"
                     /EC_number="2.4.2.9"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003869574.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_process: GO:0009116 - nucleoside metabolic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="bifunctional pyr operon transcriptional
                     regulator/uracil phosphoribosyltransferase PyrR"
                     /protein_id="HEX9917474.1"
                     /translation="MTKKDRALVMDEKAIDRALVRIAHEIVEHNRGTENMAIIGIKAR
                     GAPLAQRIAEIINQIEETKLPVGLMDITLYRDDIQTKLEQPLVQKTEIDFDVVDKIII
                     LVDDVLFTGRTIRAALDQIIDFGRPKSIQLAVLIDRGHRELPIRADYVGKNIPTAFDD
                     LVEVKIKETDGEDSVGVIKKTTK"
     gene            complement(1730..4882)
                     /locus_tag="VGB16_07020"
     CDS             complement(1730..4882)
                     /locus_tag="VGB16_07020"
                     /inference="COORDINATES: protein motif:HMM:NF012622.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="dockerin type I repeat-containing protein"
                     /protein_id="HEX9917475.1"
                     /translation="MGKVGLFLGALMLVFYPSFALGGPRISDPNYPDTVKVEQKLTVG
                     PSQHVTVTLNVDNDEKLGGIVIPLVFKNPDNPDVKCDSIHWAPRFTANPATSYAGDPT
                     TPNYVDTANYKINIWAVWYSTPFDTGNGLLCTIYFTTGSSWNSDIGIIVDSTSIPPTN
                     QLEFSDVGGGSFTPVFQPGCLGAAFEILDPNGGEVWYVGENKDILWRSVGFTGSVKLE
                     NSINSGSTWSTVIASTGNDGIHTWTIPNTPSANCRVRVSDATDGSPKDASDANFSIPD
                     FTIAGTPSYRVIDIGTSTSYTVNLGYLYGFAHPLTLSVSGLPSGGSASFTPNPVNPPT
                     ASATMNVNTTGSTPAGTYTLTIQGVGSQTHTTNVTLVVNAAPAAFTLISPANDTTVSI
                     LTPTLRWHKATDLDPLDTIKYNVYYTLKSDFSTSDSITNISDTSRVLPTLQDDTVYYW
                     KVKAIDKWGKTTWSNNTWNFRVYYPQLPAAFNLVHPPDRDTSWQLSDSLIWRKTTDPD
                     PGDSVLYDLYIDSRSGFPSPVIIANLSDTSYYFTGKDDSTYYWKVLAKDINTAGRWST
                     QTTWRFNIYVPQAPNPFSLVSPDDEDIVILHPKLYWHKATDPDPSDQLSYKVFWSLDS
                     LVYTDSSAAVTDTFYALPGLINDTIYYWKVRVKDKFNLTTWCTEAYRSFRALNVAPGS
                     FALISPTNGGTVSVLTPTLGWHKPVDPDPLDPISYTVYYSRDNTFAVFDSALSTDTSK
                     TLPTLYDDSTYYWKVKAKDSYGATSWSIDPFWSFKVYYAQEPQVFTLLLPLHNSTSSD
                     STVNFIWQSTTDPDPGDSILYDLYYDQNSGFPAPTIISNLKDTTRTVTLRDDKSYYWK
                     VLAKDINTSGRWSTGTFKVIIYVPQAPKPFSLLLPTNEDTISSLQSTLVWHKATDPDP
                     GDVIKYDLYYDITETFSAPVIVANLNVNDTTYTTPSLEPGIFYWWKVKAKDNNTSGTW
                     STQTFKFYVPSCLPGDANADTKVNVTDVIYLINYLFKGGPPPQPISACGDFQCDGKVN
                     VTDVIYLINYLFKGGPPPGC"
     gene            complement(5036..6295)
                     /locus_tag="VGB16_07025"
     CDS             complement(5036..6295)
                     /locus_tag="VGB16_07025"
                     /inference="COORDINATES: protein motif:HMM:NF012622.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="dockerin type I repeat-containing protein"
                     /protein_id="HEX9917476.1"
                     /translation="MKKVSLFFAVLMFCFSSFALAQDPGIPDTCKLDTITVTAPGQHF
                     LLQVRAYNDASFAGLAIPLKFSGSSVVCDSGSFAGTRCSTATPGVSGFTIDNTEQSVV
                     VFAVFFGVSPSPGEGPIANLYFTVRNDAINETVYVDTFTTPGGSSLEFSNPSGGSYTP
                     VFKKGKIIVKFPGPEKHNPELIAPIAKEVLCDSNGCDSLNFLVIATDLDASDILTIAK
                     YGKGSFNTTSHPSPDTGFFKWTPVRADTLNSPYVDTFMVDDGTGLADTSLVTIRVKPR
                     AVIPPSGHEGDLDGDGKINATDVIYLINFLFKSGPAPVPPSAGDINGDCFTTVEDVMY
                     LISYLYKGGPAPRIRCNPGDADYDGYVNVTDVVYFINYLFRNGPTPRSMKSCDVNADC
                     EVNVTDVVYLINYMFRNGPALLPGCVE"
     gene            complement(6446..7591)
                     /gene="mltG"
                     /locus_tag="VGB16_07030"
     CDS             complement(6446..7591)
                     /gene="mltG"
                     /locus_tag="VGB16_07030"
                     /inference="COORDINATES: protein motif:HMM:TIGR00247.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008932 - lytic endotransglycosylase
                     activity [Evidence IEA];
                     GO_process: GO:0009252 - peptidoglycan biosynthetic
                     process [Evidence IEA];
                     GO_process: GO:0071555 - cell wall organization [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="endolytic transglycosylase MltG"
                     /protein_id="HEX9917477.1"
                     /translation="MNKKDNKVEVNFWDYLIFVLSSIGIFLSSIILALSSFLFFSFKF
                     LTRRRSWLILVLALVTFASAFLIYWFIFPLPWKVSQESLSLMVKEGVNMRQVVGQLKE
                     LGIISEGRVFILVGKITGLDRKIQPGRYDFHKGITRYSLLGKLVRGEIVYTDVTIPEG
                     LTIRQIAGILKNSIGTDSAEFVRITHDRRLIDSLGMESSNLEGYLFPNTYNLYPGIPN
                     SKILEEMIGEFDKVFDENLTQRARQTGFSIHKVVTLASMIEKETQIDEERPIISSVFH
                     NRLKLGMPLQCDPTVIYALSGLDRPLYTKDLQINSPYNTYKHYGLPPGPICSPGGKSI
                     IAALYPSKTDYLYFVAKKDGNHIFSNSLAKHNRARHEARKLSATQNK"
     gene            complement(7584..8009)
                     /gene="ruvX"
                     /locus_tag="VGB16_07035"
     CDS             complement(7584..8009)
                     /gene="ruvX"
                     /locus_tag="VGB16_07035"
                     /inference="COORDINATES: protein motif:HMM:NF015607.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0005737 - cytoplasm [Evidence IEA];
                     GO_function: GO:0003674 - molecular_function [Evidence
                     IEA];
                     GO_process: GO:0008150 - biological_process [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="Holliday junction resolvase RuvX"
                     /protein_id="HEX9917478.1"
                     /translation="MSRILSIDFGEKRIGLAVSDELGITAQRLSTFHVEKDKEFFSYL
                     EEIINQKDIGQIVIGFPKMMDGTVGEKAKQVQKLAEDIKGRFGIPVILWDERLSSIQS
                     QNILREMGEKYTRKKEKVDELSALLILQNYLDYKRNRDE"
     gene            8135..9130
                     /locus_tag="VGB16_07040"
     CDS             8135..9130
                     /locus_tag="VGB16_07040"
                     /EC_number="4.4.1.-"
                     /inference="COORDINATES: protein motif:HMM:TIGR01275.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016846 - carbon-sulfur lyase activity
                     [Evidence IEA];
                     GO_function: GO:0030170 - pyridoxal phosphate binding
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="D-cysteine desulfhydrase family protein"
                     /protein_id="HEX9917479.1"
                     /translation="MRIKFPPSLNLAHLPTPIQKIKGFPGMPNDFALYVKRDDLTGFA
                     LSGNKVRKLEFVLAEALKQKADCLITCGGEQSNHARATAILATQLGLKPYLVLSAEGK
                     PKLDGNLFLDRMVGAEIRYITEDDYFNRRDQIMQVIARDLKKKEHTPYLIPEGASNTL
                     GTWGYVKAGQEIKKQIDKDNLKIERIVLASSSGGTYAGLFISSKLLNWKVKVTGFNVN
                     YDKDFVIERIWKLLVETKKRFSLEFKLDKDEIDVIDGYVGAGYAKTSDRECQLLKSIA
                     SRTGLILDPVYTGKAMFGLLDQIKKKQISEKGNILFLHTGGIFSLFAYKDKFFVD"
     gene            9294..9965
                     /locus_tag="VGB16_07045"
     CDS             9294..9965
                     /locus_tag="VGB16_07045"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEX9917480.1"
                     /translation="MELKKYQVFEGGGKMLSNGVKRGFPPERLGKNIEPKVKKDDGGY
                     YVFTLNENIKVYFEDFYKFLERVEKRSAQELEDLEEKITNTHLSRIESLSYYRARKII
                     VEHLLKQVYQLYSDGESLGVVMSPWCFGTVVLEKIELYRDRVEKGEAQDSNIPDYPYF
                     VLKYIDEIHKKTLLEIFEFPEKAFSVRWQYTELLKKYSKVLSNINTALQNILAMVKSY
                     NSSDK"
     gene            10623..11006
                     /locus_tag="VGB16_07050"
     CDS             10623..11006
                     /locus_tag="VGB16_07050"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_013969582.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="RidA family protein"
                     /protein_id="HEX9917481.1"
                     /translation="MLKKVISTNKAPLPIGPYSQAISTPQNLLFVSGQIGINPKNGEM
                     VKNGIADETKHALENLKAILEGAGSSLDNVVKTTIFLLNMEDFEQMNEVYSQYFQNDP
                     PARSTVQVTALPKGASVEIEAIAFV"
     gene            11172..11552
                     /locus_tag="VGB16_07055"
     CDS             11172..11552
                     /locus_tag="VGB16_07055"
                     /inference="COORDINATES: protein motif:HMM:NF015957.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0016020 - membrane [Evidence IEA];
                     GO_process: GO:0080135 - regulation of cellular response
                     to stress [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="PspC domain-containing protein"
                     /protein_id="HEX9917482.1"
                     /translation="MENRLYRSKKDSVIAGVCGGLGEYFGIDPVILRIVAVILVLASG
                     IGLLAYIIAWIAIPQRKDEEEAITVEKKCEANKYLPGIILVVVGLVFLLNNILPWFRF
                     DIIWPLVLVVLGIAILLKTSGGGK"
     gene            11554..12489
                     /locus_tag="VGB16_07060"
     CDS             11554..12489
                     /locus_tag="VGB16_07060"
                     /inference="COORDINATES: protein motif:HMM:NF039187.3"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF5668 domain-containing protein"
                     /protein_id="HEX9917483.1"
                     /translation="MRIYRIRNGVLLICVGFVLLLNTLDKLDWSIWWRILQLWPILLI
                     AVGIELLFKKTSLAFLGLLSPILILLAILGSVYFYHDYGQIPPFVLNLKEYKWSEKPD
                     SIVTKATVILDITGGQLDISSSQEDLIQVELKYWGRKPYCGFYYSDLDSSATLEIREN
                     NRHWANGLWKNMKKNDWNIKLSKLPLFDLRVYSAATNANLDLSEIRVEKLTLEDMGKL
                     QVKLGKLLPNIKAVIRLSELARYCASSSIPITILVPKNSGLKIISDTDLSSTSFNLRL
                     NKEKGTFFTDNFETAQNKIEVDLDGAISSLKVETY"
     gene            12607..14259
                     /gene="hutU"
                     /locus_tag="VGB16_07065"
     CDS             12607..14259
                     /gene="hutU"
                     /locus_tag="VGB16_07065"
                     /EC_number="4.2.1.49"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002033512.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016153 - urocanate hydratase activity
                     [Evidence IEA];
                     GO_process: GO:0006548 - histidine catabolic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="urocanate hydratase"
                     /protein_id="HEX9917484.1"
                     /translation="MEYKPIKAPTGNNISCKGWHQEAAMRMLMNNLDEEVGENPKELI
                     VYGGSGKAARNWECYHAIVQSLKNLENDETLLIQSGKPVGVFKTYSDSPRVLIANSHL
                     VPAWATWEKFRELEKLGLIMYGQMTAGSWIYIGTQGILQGTYETLASLAEKHFEADLS
                     GKFVLTGGMGGMGGAQPLAVTMNNGAILCVEVDEKRIKRRLDTGYCDEMSKDLDEALE
                     TILEAKKQKQPLSIGLLGNCADVLPEILKRGIIPDVVTDQTSAHDELNGYVPAGIPYE
                     KALKLRKENPEDYIKRSFDSMAKHVEAMLGFLKAGAVVFDYGNNIRGQAQKAGVKKAF
                     DFPGFVPAYIRPLFCEGKGPFRWAALSGDPEDIRKTDKVILKEFKENESLCRWIKLVG
                     KKVKFQGLPARICWLGYGQRALFGDIINDMVKKGKLSAPIVIGRDHLDCGSVASPYRE
                     TEAMKDKSDPIADWPLLNGLLNAISGASWVSIHHGGGVGIGNSIHAGMVIVADGTDEM
                     KMRLQRVLTNDPGTGIMRHADAGYDEAIKFAKEQGIKIPMMK"
BASE COUNT         4141 a         2972 c         3158 g         4037 t
ORIGIN      
        1 ctccactcaa aaggtacaaa accgccattc tgattgctac cccgttggta acctggtcaa
       61 gtatcacaga atacggtcca tccgcaactt caggagttat ctccactcct cgatttatgg
      121 gaccaggatg cataatggtg aaattttcat tcaaattttt aagtctttgc tgggttattc
      181 caaagagagt ggcatattcc cttaaagagg gaagataccc ggcttgttgc ctttctaact
      241 gaatcctcaa aacatttact atatctcttc cttggagcgc tttatccaga tctgaataga
      301 cctcaactcc catcttctca gcttcccagg gcagaagcgt ggttggtccg cagatggcaa
      361 ctgaggcccc catctttttg aagccccaaa tgcctgagcg ggcaacccgg ctatgcaaaa
      421 tatctcctac gaataacacc ctcaatccct ctatcttttt gtatttctgg cgaatggtga
      481 acaggtctaa aagcccttga gttggatgtt catgcgctcc atccccggca ttgatgatgg
      541 aggaatccag gcactgagtc aaaaaatatg gcactccaac tgatttgtgc cgcaccacca
      601 ccatatccac cttcatcgcc tcgatatttt ttactgtgtc ctttaaggtc tccccttttt
      661 taacactgga ggtgctggtg gaaaagttga tggtgtcagc agagagcctt ttttcagcta
      721 attcaaagga gatgcgggtg cgggtggacg gctcgtagaa taagttgact acggttatcc
      781 ccctcaaagt agggactttc ttgataggtc tttccagaac ctccctgaaa ctctccgctg
      841 tatctaagat taagcctatc tcccttctgg ttatcttttc taagccaata aggtgtttac
      901 ttttcagttt catctaatcc tcatttaccc tattttgttg ttttcttgat tacgcccacg
      961 ctatcctcac catctgtttc ttttatcttc acttccacta aatcgtcgaa cgcagtggga
     1021 atatttttgc ccacataatc cgctcttatg ggaagctctc tgtgtccacg gtcaatcaga
     1081 accgctaact gaatggactt aggtcgtccg aaatcaatta tctggtctaa ggccgccctt
     1141 atggttctac cggtgaaaag cacatcatcc accaagatta taatcttatc caccacatca
     1201 aaatctatct ccgtcttctg caccaagggc tgctccagtt tggtttgaat atcatcccga
     1261 tagagagtga tatccatcaa tccaaccggt agtttagttt cctctatctg atttattatc
     1321 tctgcgatcc tctgtgccaa aggcgctcct ctggctttga tgccgattat ggccatattc
     1381 tcagttcccc tgttatgttc tactatttca tgggcaatgc gaactaaggc acgatcaatt
     1441 gccttctcat ccatgaccaa agctctgtcc ttcttagtca ctcctcctcc tcctcctcct
     1501 ttttggcaaa aaaataaccc tccgcttgaa agcgaagggt tttaatttcg agattgttgc
     1561 ttagtttcat ttcaattcct tttctagcct cgctggacta gtttaaaagg taaaactaat
     1621 ataaaagcaa attcgacctt gtcaagatct attttaggat ttctttacct cgcagaaaaa
     1681 actctttagt ttccctcctt cattgcttat ccaaaaaacc cctttccacc taacatcctg
     1741 gtggcggtcc acccttgaaa agatagttaa tcaggtaaat cacatccgta acattaacct
     1801 tgccatcgca ttggaaatct ccgcacgctg agatgggctg cggaggagga ccacccttga
     1861 aaagataatt aatcaagtag atcacatccg ttacattaac cttagtatcg gcgtttgcat
     1921 cacccggcag gcaggaaggc acgtagaatt tgaatgtctg agtggaccag gtcccggagg
     1981 tattgttatc cttggctttg accttccacc agtaaaaaat tccaggttcg agtgagggag
     2041 tagtataagt tgtatcattg acgttgagat tagccactat aaccggtgcg ctgaaagttt
     2101 cagttatgtc atagtagagg tcgtatttga tgacatcccc cggatcaggg tcagttgctt
     2161 tgtgccagac caaggtagac tgtagactgg atattgtatc ttcgttagtc ggtaaaagca
     2221 aactaaacgg tttgggtgct tggggcacat agataatcac tttaaatgta ccagttgacc
     2281 atctacctga agtgttgata tcctttgcca gaactttcca gtaatatgat ttgtcatccc
     2341 gtagcgtaac tgttctggta gtatctttaa gatttgagat aatagttgga gcagggaaac
     2401 ctgaattctg gtcgtaatac aggtcgtaga gtatcgaatc acctggatcc ggatcggtgg
     2461 tagattgcca gatgaagtta acagttgagt ccgatgaagt agagttatgg agtggtagta
     2521 gtaatgtgaa aacctgaggc tcttgcgcat aatacacctt gaaactccag aaaggatcaa
     2581 tactccaact tgtcgctcca taactatctt tggctttgac cttccagtaa taggtggaat
     2641 catcgtaaag tgtgggtaga gtttttgaag tgtcggtgga taaggctgaa tcgaagactg
     2701 caaaagtgtt atcgcgggag taataaactg tgtaacttat cgggtcaagt gggtcgggat
     2761 caactggttt atgccatcct aaggtgggag tcagaacaga tactgtgcct ccgtttgtcg
     2821 gagagataag agcaaacgaa ccgggggcaa catttaatgc tcggaaactc cggtatgctt
     2881 cagtacacca ggtggtcaga ttaaatttgt cttttaccct tactttccag tagtagatag
     2941 tgtcatttat caacccaggc aaagcataga aggtatccgt cacagctgcg gatgaatctg
     3001 tatagaccag agaatcaagt gaccagaaaa ctttgtaaga taactggtca gaagggtcag
     3061 ggtctgttgc cttgtgccag tacaatttgg gatgcaagat tactatgtcc tcatcatccg
     3121 gcgagactaa gctgaaagga ttaggagctt gcggcacata aatattaaat cgccaggttg
     3181 tttgggtgct ccagcgaccg gcggtattga tatctttggc taagactttc cagtagtagg
     3241 tgctatcgtc tttgccggtg aaatagtaag aagtatcgga taagttggca ataataactg
     3301 ggcttgggaa acctgaacgg ctatcaatat aaaggtcata caacacggaa tcgcccggat
     3361 ccggatcagt agttttgcgc cagattaagc tatctgaaag ctgccagctt gtatccctgt
     3421 caggtggatg gaccaaattg aaggcagcgg gtagttgcgg gtaatagact ctgaaattcc
     3481 aagtgttgtt gctccaggta gtctttcccc atttatctat tgccttaact ttccagtaat
     3541 agacagtatc atcttgcagt gttggtaaaa cacgcgaagt atcactaatg ttagtgatag
     3601 aatcagaagt gctgaaatcg cttttcaggg tataataaac attatatttt atagtatcca
     3661 gaggatcaag gtcagttgcc ttatgccagc gaagagtagg agttaagatg gagacggtgg
     3721 tgtcattagc aggcgaaata agagtaaaag cagctggggc agcattgacc acaagggtca
     3781 cattagtggt gtgagtctga cttcctacac cctgtatagt cagcgtatag gttccagcag
     3841 gagtggagcc ggttgtattc acattcatcg tcgcagaagc ggtcggtgga tttaccgggt
     3901 taggggtgaa acttgcactt cctcctgaag gaagtccgga tacggataag gtaagagggt
     3961 gggcaaaacc atataaataa cccaaattca ctgtatagct ggtagaagtt cctatatcta
     4021 tcacccggta ggaaggggtt cctgcgatcg tgaaatcggg tatcgaaaag ttggcatcgc
     4081 tggcatcctt aggagagcca tcagtcgcat ctgagactct tacccggcaa ttggcagagg
     4141 gtgtatttgg tatagtccag gtatggattc cgtcattacc ggtgctggct ataacggttg
     4201 accaggtaga tccagaatta atcgaatttt caagtttaac acttccagta aagccaactg
     4261 atctccagag tatatcctta ttctccccca cataccagac ctctcctcca tttggatcca
     4321 gaatctcaaa agctgcgcct aaacatcccg gctgaaacac aggtgtgaag ctacccccgc
     4381 ctacatcaga aaactctaat tgattagtag gtggtataga agtggaatcg actattatcc
     4441 cgatatccga gttccaactt gagccggtgg taaaatagat tgtgcataac aaaccattgc
     4501 cggtgtcaaa aggcgtgcta taccatactg cccagatatt gattttataa ttagcagtat
     4561 cgacatagtt aggggtagtc gggtctccag cataacttgt agccgggtta gccgtgaatc
     4621 ttggtgccca atgaatcgaa tcgcatttca catccggatt gtctggattt ttaaaaacta
     4681 aggggataac aattcctccc agcttttcgt cattgtcgac gttgagtgtg acagtgacat
     4741 gctggctggg acctacggtt agtttttgtt cgactttaac agtatctgga tagttgggat
     4801 ccgaaatccg aggaccacct aaagcaaagg aagggtaaaa aacaagcatc aatgccccca
     4861 aaaatagacc tactttaccc atactagacc tccttgttat tatgggttta aggacaagta
     4921 ccctatcgtc aggtcatatt ttcgagtatt tgtgcgaaga gaggggtcaa gctcctttct
     4981 tagaagactc taaggacgtt cccccatatt caaatggagt tacttccttt ggaatctatt
     5041 ccacacaccc cggtaggagt gcagggccgt tgcggaacat ataatttatc aaataaacta
     5101 catccgtgac atttacttca caatctgcat tcacatcaca agatttcatc gatctgggag
     5161 taggtccatt cctgaaaaga taattaatga aataaacaac gtccgtaaca ttcacatacc
     5221 catcataatc tgcatctccc ggattgcatc ttatcctcgg agcaggacct cccttataca
     5281 gataactaat taaatacatt acatcttcca ccgtagtgaa gcaatcgcca ttaatatccc
     5341 cagctgacgg tggaacgggc gcaggaccac tcttgaaaag gaagttaatc aggtaaatta
     5401 catccgtcgc attgatcttg ccatctccat ccaaatcacc ttcatgcccg ctaggcggga
     5461 tgacagcccg gggtttcacc ctaatggtaa ctaaagaggt atcagccagt cctgttccat
     5521 catcaaccat gaaagtatcg acatagggag aattcaacgt gtcggcacga accggagtcc
     5581 atttgaaaaa cccggtatca ggtgaaggat gggaagtggt attgaagctg cccttcccat
     5641 atttggcgat ggttaggata tcggatgcat ccaggtctgt ggcaataacc aaaaaattca
     5701 gactatcaca tccatttgaa tcacacaaga cctctttggc aatcggagcg ataagttctg
     5761 gattatgttt ctctggccct ggaaacttta ctattatctt gccctttttg aagactggcg
     5821 tatatgaacc accactagga ttagagaact ctaacgaaga acctcctggg gtagtaaaag
     5881 tatctacata aaccgtttcg tttatcgcat catttctaac ggtaaaatag agattagcaa
     5941 taggaccttc tcccggagaa ggactaactc caaaaaagac ggcaaaaaca actacacttt
     6001 gctcagtatt atcaatagtg aaaccgctga caccaggggt tgcagttgag cagcgcgtgc
     6061 cagcaaaaga accggaatca caaaccactg aactaccaga gaactttaga ggaattgcaa
     6121 gtccagcaaa acttgcatca ttgtaagccc taacctgcag cagaaagtgc tgacccgggg
     6181 cagtaacagt gatagtgtca agcttacagg tatccggaat cccggggtct tgagccaggg
     6241 caaaagaaga aaagcaaaac atcaatactg caaaaaacag actaactttt ttcattacag
     6301 gcctcccttt aattttgttt taggagatag ttttcaataa cgataatata accaaaatcc
     6361 gaattttgtc aataggttat tttcacttaa gaacaaccag taatctctga aggtaaagaa
     6421 ggcgtaggaa ggttggtctg atgctctatt tgttttgggt tgcagacaac tttcttgcct
     6481 cgtgtcgggc gcggttatgc ttggctaagc tattggaaaa tatatggtta ccatcctttt
     6541 tagccacaaa atacaaataa tctgttttcg aaggataaag cgcggctatg attgattttc
     6601 cccccggact gcagatcggt cctggcggaa gaccataatg tttgtaggtg ttgtagggtg
     6661 aatttatctg caagtctttg gtataaagcg gcctatccaa gccggacaac gcatagatta
     6721 ccgtggggtc gcattgtaac ggcataccta atttcaacct gttatgaaaa acagaagaaa
     6781 taatcggtct ttcttcgtca atctgggtct ccttttctat catcgaagct aaagtgacta
     6841 ctttatgtat actaaaacct gtctgtcttg ccctctgagt tagattttca tcaaatactt
     6901 tgtcgaattc gccgatcatt tcctccaata ttttactatt gggtatgccc ggatacagat
     6961 tataagtatt cggaaaaaga tacccctcca aattagaaga ttccatcccc aagctgtcaa
     7021 tcaatctcct atcatgggtg attcggacga actccgctga atctgttcct atactatttt
     7081 tcaggattcc ggctatttgt cttatagtta aaccttcggg tatggtaaca tcagtataaa
     7141 ctatctcccc cctgaccaac ttccccaaaa gagaataacg ggtaatccct ttatggaaat
     7201 catacctgcc cggctgaatc ttcctgtcca acccagttat ttttccaact aagataaaaa
     7261 ctcttccctc cgaaattata ccgagttctt tcaattgccc aactacctgg cgcatattca
     7321 caccctcttt aaccatcaaa gatagcgact cttggcttac tttccagggt agcgggaaga
     7381 taaaccagta gataagaaaa gcagaagcaa aggtaaccaa agccaaaacc aagataagcc
     7441 aggatcttct tcgagttaag aacttgaagg aaaaaaataa gaaagaggac aacgccagta
     7501 tgattgaaga taagaatatc cctatgctgg acaacacgaa tatcaagtaa tcccaaaaat
     7561 tcacttctac cttattgtct tttttattca tctctgtttc ttttgtaatc cagatagttc
     7621 tgtaatatca acaaagcgga aagctcatcc accttctctt tttttcgagt gtatttttcg
     7681 cccatctccc ttaagatatt ttgagattga attgagctta acctttcgtc ccatagaatt
     7741 accggaattc cgaacctgcc ctttatatct tcggcaagtt tttgaacctg tttggctttt
     7801 tctcccactg tcccatccat catcttagga aagccgatta ctatttgtcc tatatccttc
     7861 tgatttatta tctcttccag gtaggagaaa aattccttat ccttctcaac gtgaaaagtg
     7921 gataatctct gggcagttat tcccagctca tcggaaacag ctaatcctat cctcttttcg
     7981 ccaaagtcta tggataaaat gcggctcata actcattcaa tttaaactct aactgatttt
     8041 tgtcaaagga aaactatttg ctttttgagt ctatgcttga caaatgaccc aagtcaaatt
     8101 aaattaggta taatttttta gaaaaggatg gataatgaga attaaatttc ctccatctct
     8161 taacctggct caccttccta cccccattca aaaaatcaaa ggctttcccg gtatgccaaa
     8221 tgattttgcc ctctacgtta aaagggacga tttaactggc tttgccttat caggaaacaa
     8281 agtcaggaag ctcgaatttg tcttggctga ggctctaaaa caaaaagctg attgcttaat
     8341 aacctgtggc ggagagcaat ccaatcatgc ccgcgccact gcaattttgg caacacagct
     8401 gggactaaaa ccatatttag tcctctctgc tgaaggcaag ccgaaattgg atggaaatct
     8461 ctttttggat agaatggtag gggcagagat acgatacata actgaggatg attactttaa
     8521 ccgaagagac cagataatgc aggtaatcgc tcgtgacctg aaaaagaaag agcatacacc
     8581 atatctgatt cccgaagggg catccaacac cttgggcacc tggggatatg tcaaagctgg
     8641 tcaggagata aagaagcaaa tagataagga taatctaaaa atagaacgaa tagttctggc
     8701 atccagctcc ggtggcactt atgctggttt gttcatcagt tcgaagcttc tgaattggaa
     8761 ggttaaggta accggcttta atgttaacta tgataaggat tttgttattg agcgcatctg
     8821 gaagcttttg gttgaaacta aaaagagatt tagtcttgag tttaaattgg ataaagatga
     8881 aattgacgta attgacggat atgttggggc aggatacgcc aaaacttcag atagggaatg
     8941 ccagcttttg aaatcgattg cctcacgtac cggcttgatt ctcgacccgg tttacactgg
     9001 caaagctatg tttggccttt tggaccagat aaaaaagaaa caaatatcgg aaaaaggaaa
     9061 cattctgttt ttgcacactg gcgggatttt cagcctattt gcctataagg acaagttttt
     9121 tgttgattaa gtaacatctt gcaactatta tgaagtagat tgcatagcgg atatggattg
     9181 aaatagaaac tcaaaaaaga ataatcatct taagcggttg cttattaagg tattaactaa
     9241 tataaattta ggtttgagcc agagaatctt tgtggcatat tttttgctga aatttggaac
     9301 tgaaaaagta tcaggttttt gaaggtggag ggaaaatgct atcaaatggc gtaaaaaggg
     9361 gattcccgcc agaaagatta ggcaaaaaca ttgagcccaa ggtgaagaaa gacgacggag
     9421 ggtattacgt ttttaccttg aacgagaata ttaaggtcta tttcgaggat ttctataaat
     9481 tcctggaacg agttgagaaa aggtcagccc aggagctgga agatttagag gaaaagataa
     9541 caaataccca cctctctcgc atagagagtc tttcttatta tcgcgcccgc aagataatcg
     9601 tggagcacct tttgaaacag gtttaccaat tgtattcgga tggtgagagc ttaggagtag
     9661 ttatgtcgcc ttggtgcttc ggcactgtgg ttttggaaaa aatagagctt tatcgggata
     9721 gggtcgaaaa gggagaagcg caagattcca acattcctga ctatccctac tttgtgttaa
     9781 agtatataga tgagattcac aagaaaactc ttttggagat atttgaattt ccggaaaaag
     9841 ccttcagcgt gagatggcaa tataccgagc tgttgaaaaa atattctaag gtcctctcca
     9901 acatcaatac cgccttgcaa aacatcctgg ctatggtgaa aagctataat tcctcggata
     9961 agtgaagcct ccccttgata aatcacgggg cctcccaaat ataaatcccg tctgtggcgg
    10021 gacttatggg ccgaatctag aaccgctcga tccttcgacc cttcgatgaa ctcaggatgc
    10081 tgagcaaagt cgaagcacta gctcaggacg gtgagtttat cgaaccgtaa atcgagcaac
    10141 tacaaacttc taaacatttt aaactgctaa actgatttca tgtagggaca gaacaatgtt
    10201 ctgtccattt cttttgcagg cgacccagat gggtcgcctg caatttctta catacatttg
    10261 ttagcagggc taaatgccct gctctacgag gagtttcatc tttgtagggg caattcatga
    10321 attgccccta ccgttttgat tcctgttttt gtttgtcatt cctgccccgc attcctgcgg
    10381 ggtgaactcc ggcaggaatc cattgggttt taaaaaaatc gaaaacctat caggtactcc
    10441 actgttatga accgaaatgg ttcgtaccag tggcatccgc cactgggctc gagtcatttt
    10501 ttcagtcgta gggcgggaaa ggtgcccgcc gaaactcacg ctttttctat aaaacattaa
    10561 aaataatctt gacacccaga aaatttgatt tatttttcaa actgtttttt gaaggaggtg
    10621 ctatgctcaa aaaagttatc tccactaata aagccccttt acccatcggt ccctactctc
    10681 aagccatatc aactccccaa aatctccttt tcgtttccgg gcaaatagga attaatccta
    10741 aaaacgggga aatggttaag aatgggattg cagatgaaac caaacacgct ttggaaaact
    10801 taaaagccat actggaagga gcaggctcaa gtttagataa cgtggttaag actacgatct
    10861 tccttttgaa tatggaggat ttcgagcaga tgaatgaagt ctattctcaa tacttccaaa
    10921 atgacccgcc tgcccgttcc accgttcaag tgactgccct gcctaaaggc gcaagcgtag
    10981 agattgaagc aattgctttt gtttgaaaac aaaagaaacc tgtagctcga aaattccgta
    11041 ttgaagatgt gaagcctccc cgtgataaat cacggagctt ccaaaatata aaaggccctc
    11101 ggcttccctc ggcttccctc ggcttcgccg agaggcgagc gccgagaggc gagtgaaacc
    11161 aggggatcaa aatggaaaat agactttatc gttccaaaaa ggattcggtc atcgccggag
    11221 tgtgcggtgg actgggtgaa tatttcggca tcgacccagt tatcttaagg attgtagcgg
    11281 tcattctggt tctggctagc gggattggac tgttggctta cattatcgcc tggattgcca
    11341 ttcctcaaag aaaagatgaa gaggaagcga taacggttga gaagaagtgc gaggcgaata
    11401 agtatttgcc gggaataatt ttagtggtag tcgggcttgt ttttctgctt aacaatattc
    11461 tcccctggtt tcgttttgac ataatttggc cacttgtcct tgttgttttg ggcattgcca
    11521 tcttgcttaa gacctcaggc ggaggaaaat gaaatgagaa tctaccgcat aagaaatggg
    11581 gttttgctta tctgtgttgg ttttgtcctg ctcttaaata ctctggataa attggattgg
    11641 tcaatctggt ggcgaatttt acagctgtgg ccaatccttt tgattgctgt tggaattgag
    11701 cttttgttca agaaaacctc tctggctttt ctgggattac tctcacctat tttgatactt
    11761 ctggctatac ttggatcagt ttatttttat cacgattatg gtcaaattcc tccctttgtt
    11821 ttaaacctaa aagagtacaa atggtcggaa aaaccggata gcatagtcac caaagccaca
    11881 gttatattag acatcactgg ggggcagttg gatatctcaa gcagtcagga ggatttgatt
    11941 caggtggagc tgaaatactg gggaagaaaa ccttattgtg gattctacta ttcggatttg
    12001 gatagctctg ctactttgga aataagagag aacaacaggc attgggcaaa tgggttgtgg
    12061 aaaaatatga agaaaaacga ttggaacata aaactaagca aactacccct tttcgatttg
    12121 agggtatatt ctgccgctac taatgctaat ctggatttat ccgaaatcag agtggaaaag
    12181 ttgacgttgg aggatatggg gaaattgcag gtcaaattgg gaaagttact tccaaatatc
    12241 aaagcggtta ttcggcttag tgagttggct cgatattgtg cttcctcgtc tatacctatt
    12301 actatcttag tgccaaagaa ttcgggactg aaaataattt ctgatacaga tttaagctct
    12361 acaagtttta atcttaggct gaataaagaa aaaggtacat tttttacaga taatttcgag
    12421 accgcacaaa acaagattga agtggactta gatggcgcca tttcgtcctt gaaagtagag
    12481 acttattgat atttactact aatgtcaaat gacgaccgcc ttatttgtta gggcggtttt
    12541 ttattgactt aaaatcttgt gggtattaat ttgattttgt attactgata aaaacggagg
    12601 taggatatgg aatacaaacc tatcaaagcc ccaactggaa ataacatctc ctgcaagggg
    12661 tggcatcagg aagcagctat gcggatgctg atgaataacc tggatgaaga ggttggcgaa
    12721 aatccaaagg aactaatcgt ctatggtgga agtgggaaag cggctcgcaa ctgggaatgc
    12781 tatcacgcga ttgtgcagag cttgaagaat ctggaaaatg acgaaaccct gctaattcaa
    12841 tctggaaagc cggtgggggt ttttaaaacc tattcagatt ctccgcgagt cttgatcgct
    12901 aattcccacc ttgtcccggc ttgggcaacc tgggaaaaat tcagggaact ggaaaaatta
    12961 ggtcttataa tgtatggaca gatgactgct ggaagctgga tttatattgg aactcaaggg
    13021 atcttacagg gaacttacga gaccttagcc agcttggcgg aaaaacattt tgaggccgac
    13081 ctttctggaa aattcgtttt aactggaggg atgggaggaa tgggcggagc ccaacccttg
    13141 gcagtaacta tgaataacgg ggcaattctc tgtgtagagg tggatgagaa gaggataaaa
    13201 agaagactgg acaccggtta ttgcgacgag atgtcaaaag atttggacga agctttagaa
    13261 acaattctag aagcaaaaaa gcaaaagcaa cccctctcca ttggactgct tggcaactgt
    13321 gctgatgttc tgccggagat attaaaaaga ggaattatcc cggatgtggt aactgaccag
    13381 accagtgcgc atgacgaatt aaatggatat gttccagctg ggattcctta tgaaaaagct
    13441 ttgaaactga ggaaagaaaa tccagaagat tacatcaaaa ggtctttcga ttcaatggca
    13501 aaacatgtcg aagctatgct tggattcttg aaagccggag cagttgtttt tgattatgga
    13561 aacaatatca gagggcaagc ccagaaagct ggagttaaaa aggcttttga ttttcccggg
    13621 tttgtgccgg cttatattag gcctcttttc tgtgagggaa aaggtccttt tcgctgggcg
    13681 gctctttccg gagacccgga ggatatccga aaaactgata aggtcatctt aaaagagttc
    13741 aaggagaatg aatctctgtg tcggtggata aaattggttg gcaagaaagt gaagtttcag
    13801 ggcttgcctg ccaggatatg ctggttagga tatggacaac gtgctctttt tggagatatc
    13861 ataaacgata tggtcaaaaa aggtaaactt tctgctccga ttgtgattgg cagagaccat
    13921 ctggattgtg gctcggttgc ctcaccttac agggaaaccg aagcaatgaa agataaatca
    13981 gacccaatcg ctgattggcc gcttttgaat ggcttactca atgccatctc cggcgcatcc
    14041 tgggtttcaa tccatcatgg aggcggagtg ggaatcggca actctattca tgctggaatg
    14101 gtaattgtgg cagacggaac agatgagatg aaaatgcgat tacaaagagt tttaaccaat
    14161 gaccccggaa caggaataat gcgccacgcg gatgctggtt atgatgaggc gattaagttt
    14221 gccaaagaac aaggaattaa aatcccgatg atgaagtgag tttcactttg aataaccaaa
    14281 agatttttgt ctgtcattcc tgcgtagg
//
LOCUS       DASWLN010000191         6664 bp    DNA     linear   ENV 20-FEB-2024
DEFINITION  MAG TPA_asm: Candidatus Zixiibacteriota bacterium isolate
            SMAG_U2949 k141_1263374, whole genome shotgun sequence.
ACCESSION   DASWLN010000191 DASWLN010000000
VERSION     DASWLN010000191.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37960594
            Sequence Read Archive: SRR10376091
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Candidatus Zixiibacteriota bacterium (soil metagenome)
  ORGANISM  Candidatus Zixiibacteriota bacterium
            Bacteria.
REFERENCE   1  (bases 1 to 6664)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 6664)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (26-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/12/2024 08:28:26
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 1,440
            CDSs (total)                      :: 1,416
            Genes (coding)                    :: 1,412
            CDSs (with protein)               :: 1,412
            Genes (RNA)                       :: 24
            rRNAs                             :: 1 (16S)
            partial rRNAs                     :: 1 (16S)
            tRNAs                             :: 21
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 4
            CDSs (without protein)            :: 4
            Pseudo Genes (ambiguous residues) :: 0 of 4
            Pseudo Genes (frameshifted)       :: 0 of 4
            Pseudo Genes (incomplete)         :: 3 of 4
            Pseudo Genes (internal stop)      :: 1 of 4
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..6664
                     /organism="Candidatus Zixiibacteriota bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_1263374"
                     /isolate="SMAG_U2949"
                     /isolation_source="soil"
                     /db_xref="taxon:2053527"
                     /environmental_sample
                     /country="USA: Oregon"
                     /lat_lon="42.99 N 123.63 W"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            complement(99..830)
                     /gene="truA"
                     /locus_tag="VGB16_07070"
     CDS             complement(99..830)
                     /gene="truA"
                     /locus_tag="VGB16_07070"
                     /EC_number="5.4.99.12"
                     /inference="COORDINATES: protein motif:HMM:TIGR00071.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0009982 - pseudouridine synthase activity
                     [Evidence IEA];
                     GO_process: GO:0006400 - tRNA modification [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="tRNA pseudouridine(38-40) synthase TruA"
                     /protein_id="HEX9917485.1"
                     /translation="MQRNIRLEIEYDGTNFSGWQIQPKRRTVQREIENRLEKLLREKI
                     NIIGAGRTDAGVHALAQVANFKTKSKLKIENILRGLNSLLPADIVIKNIKEVDEKFHA
                     RFCAKSRVYKYRLYSGKTALLRDFVWETGYHLNLDKMKKATRKILGKHDFSSFCVTKS
                     SKENNICKVLKALWRKKGNELVFEIEADRFLHSMVRALVGTMVDVGRGYFSISDFQKI
                     LLSKDRKKAGLTAPAKGLYLVKVKY"
     gene            complement(833..1621)
                     /locus_tag="VGB16_07075"
     CDS             complement(833..1621)
                     /locus_tag="VGB16_07075"
                     /inference="COORDINATES: protein motif:HMM:NF014420.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_process: GO:0055085 - transmembrane transport [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="energy-coupling factor transporter transmembrane
                     component T"
                     /protein_id="HEX9917486.1"
                     /translation="MNCLENFSLGQYFPSDSVIHKLDARTKLVLILSIILPAALIVNL
                     HVYLFLLVLLLLIYAFSKLRLFQLWGNFRAFLWLFVFIFGVHLLYNPQTKAIPNLTME
                     GLSNGILFSLRLGIFVLGALVLSFTTPAVELTDGFFKFLSPLKKLKLPIEELSLMMMI
                     ALRFIPLLIEEAFTLKKAQEARGADFEGNLLKRSRKLVPLLLPLFVSSFRKAEDLALA
                     LDARGFRSGAKRSSFRINRFGLADYLFFGLSLLVLVISWSINKF"
     gene            complement(1624..2748)
                     /locus_tag="VGB16_07080"
     CDS             complement(1624..2748)
                     /locus_tag="VGB16_07080"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_013276942.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF362 domain-containing protein"
                     /protein_id="HEX9917487.1"
                     /translation="MPSKVYFINARVEKFDYKYSLLGRLEKVLKDIDLSKYFSGGELV
                     PIKMHLGNKGAHSTIRPNFVKLVIDKMKEVGAKPFVTDSARVKPYEYLEVANEMGYNS
                     LTLGVPVIIADGIFGFDSIKVKAGKMLKEIAISSAIHDASAMMVLTHVKGHIDSSLGG
                     AIKNLAMGCVSALPRSCHWKEGGRGKMHFLMWDIMEWDAKLCTFCGICANNCPMDAIT
                     IKARVFEVDDKKCVRCGRCVKVCPEGALKIPFSAENFQKGMAESVKAVLSTFKPGKVL
                     YVNFILDVQPECDCMLVTDTPVIQDQGILVSDDIVAIDQATMDIIQKAAPLPQSKAEG
                     IEFGKFEDILSAIHQKDAKIQIKAAEKLGLGSRKYKLIEL"
     gene            complement(2764..3951)
                     /locus_tag="VGB16_07085"
     CDS             complement(2764..3951)
                     /locus_tag="VGB16_07085"
                     /inference="COORDINATES: protein motif:HMM:NF013237.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="aminotransferase class I/II-fold pyridoxal
                     phosphate-dependent enzyme"
                     /protein_id="HEX9917488.1"
                     /translation="MSKSQKHSFSTLCIEAGEEDKFPLGAVTKPIYQTSTFAFRDTAE
                     VIKFQKGDEGKYLYTRYGNPTLKVVEDKMAALEGGEKGLVVSSGMAAVSTVMFALLRA
                     GDRIISTRPVYGGTYHLFLDLLPRLGIDVKFVAVDSLGLAEKYVDKNTKLFFSETPTN
                     PNLKIVDLEKLVKLGKKHKLLTVLDNTFATPYNQNPLSLGIDIVLHSGTKYLAGHSDI
                     VCGAIVGKKKFVVRARETMKVLGGCIDPLGAFLMLRGLKTLGLRMEKHNINGYRIAQF
                     LSRHPKVRRVFYPGLRNHPQYELARKQMKGFGGMICFEVVGGLKGATKVMDNLRLFIN
                     ATSLGGVESLASIPVLTSHYGFSSRELNMADVTPGMIRLSCGIEDTQDLIDDLKGALN
                     RLR"
     gene            complement(3960..4454)
                     /locus_tag="VGB16_07090"
     CDS             complement(3960..4454)
                     /locus_tag="VGB16_07090"
                     /inference="COORDINATES: protein motif:HMM:NF020123.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016491 - oxidoreductase activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="TlpA disulfide reductase family protein"
                     /protein_id="HEX9917489.1"
                     /translation="MDRWKIVCSVLILIVSVPWYNCQKASKGSVLDVQVKGLDDKIYN
                     LTTIKGDLILVNFWATWCPPCKREIPELIKLQEEYKNKGLGVVGISVDTEGTKKVRQF
                     VEDYKINYTVFIGNQEVVQRYGGFYGIPVSFLVSKEGIIQKAYIGSLNKEIVEKDLKT
                     LLKK"
     gene            complement(4436..5956)
                     /locus_tag="VGB16_07095"
     CDS             complement(4436..5956)
                     /locus_tag="VGB16_07095"
                     /inference="COORDINATES: protein motif:HMM:NF024211.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="4Fe-4S binding protein"
                     /protein_id="HEX9917490.1"
                     /translation="MRNVRRISQILFLLFFFYLFLIASYPLNFPIPADSFLRADPLLA
                     LSSMMAVRNVIPTFWISLILIILTIPLGRVFCGWVCPLGTTLDTTSYVFRNPVGKKTT
                     NLRFLKYTLLILILVSAVFLSQLVWILDPIVLYTRTLTISLFPLLTFSTYSLFGLLFK
                     ISFLQDFLTTLQTHLASTILPNQLSFFRMSALILLIFSGILGLEFVSRRFWCRNLCPL
                     GALYSFFSKLQIWRRKVKDGCTNCADCIKVCKMDAIENDFINTKFGECIQCYNCVADC
                     PTQVTSLSFRKEPKAYSLNLTRRRVVVAGILGVLFSGVLKVGFLNKNARAELIRPPGA
                     KEEGEFLKRCIRCHKCIRVCSTSGNFLQPAILEGGWEGFWTPVGYARGGYCEYNCTMC
                     THICPSGAIHPLDEETKKKTVIGLAYIDRNRCLPWYKNQDCVVCEEHCPIPQKAIDLK
                     PEKVIDSEGKTKIIKRPYVKEDLCIGCGICETRCPLQGRSAIIVISQGEQRWTDGK"
     gene            complement(5953..>6664)
                     /locus_tag="VGB16_07100"
     CDS             complement(5953..>6664)
                     /locus_tag="VGB16_07100"
                     /inference="COORDINATES: protein motif:HMM:NF015948.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=2
                     /transl_table=11
                     /product="DUF362 domain-containing protein"
                     /protein_id="HEX9917491.1"
                     /translation="KPNIGWDRTPEQGATTNPEVVAEIVRMCLEAGAKKVKVFDNSCN
                     AAIRCYDRSGIMKAASRAGAEVSYMVEAGFSKVNLPQGEAIKSWEIYKPVLEADLLIN
                     VPIAKHHDFAGLTLGIKNLMGIMGGNRGKIHWNLNDSLVDLVNFIRPRLTILDATRIL
                     LRNGPQGGDPKDVRKLDTIIAGTEIATVDAYGTTLFGLKPQDLACIVKASKFGLGEID
                     LKRVNIRKISLAQAGDKL"
BASE COUNT         1910 a         1617 c         1185 g         1952 t
ORIGIN      
        1 gatggtgagg acattcggct gagcggttcg accgtcctga gcgaagtcga aggaaacttc
       61 gcccctacgc gtgcccattt ctttggaaca acaatatttc aatacttcac cttcactaaa
      121 tataaccctt tagctggagc agtcaagccc gctttttttc tgtctttgga taaaagaatt
      181 ttctgaaaat cggaaatgga aaaatatcct cttcccacgt ccaccatagt gccaacaagg
      241 gccctaacca tagaatggag aaagcggtcc gcctcgattt caaagacaag ctcatttcct
      301 ttttttctcc agagggcttt taagacctta cagatattat tctctttaga tgacttggtt
      361 acgcaaaagg atgaaaaatc atgcttgcct aatatttttc ttgtagcttt tttcatctta
      421 tccaaattca agtgatatcc agtttcccac acgaaatccc tcaaaagggc ggttttgcct
      481 gaataaagtc tgtatttgta aaccctgctc ttagcgcaaa aacgggcatg gaatttttca
      541 tccacctcct tgatattctt aattacaata tctgctggca gaagcgaatt caatcctctt
      601 agtatatttt ctatttttaa tttgcttttt gttttaaaat tagccacctg tgccaaagca
      661 tgcactcctg catcagtcct gccagctcca attatattga ttttttccct taaaagtttt
      721 tccaatctat tttctatttc tctctgaact gtgcgccttt ttggttggat ttgccagcca
      781 gaaaagtttg tgccgtcgta ttctatttcc agacgaatat ttctctgcat atttaaaatt
      841 tattgatgct ccaggatatg accaagacca aaaggctcaa accaaaaaat aggtaatcag
      901 ccaatccgaa tctatttatt ctaaaggagc tccttttagc tccacttctg aatcccctgg
      961 catccaaagc caaagccaaa tcctctgctt tcctgaagga ggaaacaaat aatggcaaaa
     1021 gcaggggaac taactttctc gaacgcttta gaagatttcc ctcaaaatca gctccccttg
     1081 cctcttgagc cttcttaaga gtgaaagctt cttcaatcaa aaggggaatg aatcgcagag
     1141 ctatcatcat catcaagctt agctcttcaa tgggcaattt caattttttc aagggtgaga
     1201 gaaatttgaa gaaaccatca gtcaattcaa ctgccggtgt ggtgaagctt aagaccagcg
     1261 ctcctaaaac aaatatgcct aatcttagac taaacaatat gccgttgctc agtccctcca
     1321 tagtgagatt aggaatggct ttagtttgtg gattgtataa aagatgaacc ccaaatataa
     1381 aaacaaacag ccacaagaat gccctgaaat tcccccataa ttgaaaaaga cgtagtttcg
     1441 agaaagcata gattagaagc aataagacga gtaagaatag ataaacatgc agattcacga
     1501 ttaacgccgc gggcaatatt atgctgagaa ttaacaccaa cttggtgcgg gcatccaatt
     1561 tatgaatcac agaatcggat ggaaaatatt gacctaagga gaaattttcc aaacagttca
     1621 tctctaaagc tcgatcagct tatattttct tgaacctaaa cccaatttct ccgcagcttt
     1681 aatttggatt tttgcatctt tctgatgaat ggcacttaag atatcttcga attttccaaa
     1741 ttctattcct tctgccttag attgaggtaa gggagctgcc ttttggatta tatccattgt
     1801 ggcttggtca attgccacga tgtcatcgct tactaatatc ccctggtcct gaatcaccgg
     1861 tgtatcggtt accagcatac aatcgcactc tggctgaaca tccaagatga aattaacgta
     1921 aagcactttg cccggcttga acgtgcttaa gacagctttg actgattcag ccattccttt
     1981 ttgaaaattc tccgcggaga atggtatctt aagtgccccc tctggacaaa ccttgacaca
     2041 gcggccacag cgcacacatt tcttgtcatc cacctcaaac accctcgctt tgattgttat
     2101 ggcatccata ggacagttat ttgcacagat gccacagaag gtgcacaatt ttgcgtccca
     2161 ctccattata tcccacatta agaagtgcat ctttcctctc cccccttctt tccaatggca
     2221 acttcggggt aaggcactaa cgcatcccat agccaggttc ttaattgctc ctcccaagct
     2281 cgaatctatg tgtcccttaa cgtgagttag aaccatcata gctgaagcgt catgaatagc
     2341 agaggaaata gctatctcct ttaacatctt cccggcttta accttaatcg aatcgaaacc
     2401 gaaaatccca tccgcaataa taaccggaac tcccagagtt aaagaattgt atcccatttc
     2461 atttgccacc tctaaatact catagggctt cactctggct gaatcggtga caaaaggttt
     2521 tgctccaacc tccttcattt tgtctatcac caattttaca aaattcggcc taattgtgct
     2581 gtgtgccccc ttattcccca aatgcatctt tatcggaacc agctccccgc cagaaaaata
     2641 tttgcttaaa tctatatctt ttaggacttt ctccagtctt cctaaaagac tatacttgta
     2701 gtcgaatttc tccactcgcg catttatgaa atagaccttg ctgggcatat tagcttacct
     2761 ctcctatctt aatctattta aggctccctt caggtcatcg atcaaatcct gcgtatcttc
     2821 aatgccacag gaaagccgaa tcatgcccgg cgtgacatca gccatgttta gctctcttga
     2881 actgaaaccg taatgtgaag tcaaaaccgg tatggaagcc aatgattcaa ctccgcctag
     2941 gctggtagca ttgatgaaga gtcgtaggtt atccataacc ttagttgccc cttttaaccc
     3001 tccaaccacc tcaaagcaga tcataccgcc aaatcctttc atttgctttc gggctaattc
     3061 atattgggga tgattcctca aaccaggata gaacaccctt cttacctttg gatgcctgga
     3121 gaggaattgg gctatgcgat atccgttgat attatgcttt tccattctaa gtcccagagt
     3181 ctttaaacct ctaagcatca agaatgcgcc caaggggtca atgcaaccgc ccaaaacctt
     3241 catggtctct cgagcccgga ccacgaactt cttcttacca actatcgctc cacatacgat
     3301 atcactatgc cctgccaaat atttggtgcc gctgtgaaga acaatatcta tccccaaaga
     3361 caaggggttt tgattatacg gcgtggcaaa ggtgttatcc aagactgtca agagtttatg
     3421 ttttttgcct aatttaacta gtttctccaa atccacaatt ttcaaatttg gattagtagg
     3481 agtttcagaa aaaaagagtt tcgtattttt atcgacatat ttctcagcta gtcctaaact
     3541 atctactgct acaaatttca catctatacc cagtcgggga aggaggtcta agaacagatg
     3601 gtaagttcca ccataaaccg ggcgtgtgct tattatccta tctccagctc tcagcaaagc
     3661 gaacatcacc gtgctaactg cagccatacc ggaggagacc accaacccct tttccccccc
     3721 ttccaaagct gccatcttat cttcaaccac cttcagagtc ggattgccat agcgcgtata
     3781 aagatatttc ccttcatccc ccttctgaaa cttgatcacc tcagccgtat ccctgaaggc
     3841 aaaagttgag gtttgataga tgggcttggt cactgcacct aaggggaatt tatcttcttc
     3901 tccagcttca atgcacagcg tagaaaaaga atgcttttga cttttggaca tatttgctcc
     3961 tattttttga gtaacgtttt caaatccttt tcaactattt ccttattcag ggagccaata
     4021 taagctttct gaattatccc ctctttgcta accagaaaac taactggaat accgtaaaaa
     4081 ccaccgtatc tttgtaccac ttcctgattt cctataaaca ctgtgtaatt gattttgtaa
     4141 tcttcaacaa actgtcttac tttcttcgtg ccttcggtgt ccacggatat ccccacgacg
     4201 cccaacccct tattcttgta ttcctcttgg agcttgatta gctctggaat ttcccgtttg
     4261 cacggaggac accaggtagc ccaaaaatta actaaaatca aatctccttt gattgtcgtc
     4321 aggttataaa tcttgtcatc taagcccttg acttgaacat ctaagaccga acccttagaa
     4381 gccttttggc aattgtacca aggtacactc acgattagaa tcaggactga gcaaactatt
     4441 ttccatctgt ccatctttgt tctccctgtg aaatgactat tatggctgac ctcccttgga
     4501 gggggcatct ggtctcgcat attccacatc cgatgcataa atcttcctta acataaggcc
     4561 ttttgataat cttggttttt ccttccgagt ctatcacctt ttcaggtttc aaatctattg
     4621 ccttctgtgg aatgggacaa tgctcctcac atactacaca atcctgattt ttataccagg
     4681 gtaggcatct attcctatcg atataagcta aaccgattac tgttttcttt ttggtttctt
     4741 catctaaggg atgaattgct ccgctgggac aaatgtgtgt acacattgtg cagttatact
     4801 cacaataccc tccacgagca tatcccaccg gtgtccagaa accttcccat ccaccttcca
     4861 aaattgctgg ctggagaaaa ttcccactgg tggagcaaac cctaatacac ttatgacacc
     4921 ggatgcatct tttcaaaaac tctccttcct ctttcgcacc aggtggtcta atcaactccg
     4981 ctctggcatt cttgttaaga aaacctactt tgagaactcc ggaaaagagt acacctaaaa
     5041 tccccgctac aaccaccctc cggcgagtta ggtttaggct atatgctttg ggctcctttc
     5101 taaagctaag cgaagtaact tgggttggac aatcagccac gcagttgtaa cactgaatac
     5161 attccccgaa tttagtattg ataaaatcgt tctctatggc gtccatcttg caaaccttaa
     5221 tgcagtccgc acaattagta cagccatctt taacttttct tctccatatc tgcaatttgg
     5281 agaaaaacga ataaagcgct cccagaggac aaaggttgcg acaccaaaat ctgcgtgaga
     5341 caaattccag gccaagaatc cctgaaaaga tcaacaatat taaagccgac attctaaaaa
     5401 aagagagttg attgggtaat atcgttgagg ccaagtgcgt ttgaagggta gtcaggaaat
     5461 cttgtaaaaa agatattttg aagagtaaac caaaaagact ataggttgaa aaagttagca
     5521 aaggaaaaag ggaaatagtc aaagtacgag tatatagaac aatagggtct aagatccaaa
     5581 ccaattggct caaaaaaacc gcagaaacta agattagaat caacagggta tatttcaaaa
     5641 atcgtagatt ggtagttttc tttccaaccg ggtttctgaa aacatagcta gttgtatcta
     5701 aagttgttcc caaggggcaa acccatccgc agaatacccg acccaaggga atagtcagga
     5761 ttatcaggat aagagagatc cagaatgtag ggattacgtt acgtacagcc atcatgctgg
     5821 acagggctaa aagtgggtcg gccctcagaa aggagtcagc cggtatggga aaattcaaag
     5881 gataactggc aatcaaaaaa agatagaaga aaaacaacaa gaaaagaatc tgacttatcc
     5941 tgcgaacgtt tctcaaagtt tgtctccggc ttgggctaag ctaatttttc ttatattcac
     6001 cctttttaag tctatttctc ccaatccaaa tttgcttgcc ttgacgatac acgccaaatc
     6061 ctggggtttc agaccgaaca gggtagtgcc ataagcatcc actgtggcta tctcggttcc
     6121 agcgattatc gtatccaatt tcctcacatc tttagggtca ccaccctgtg gtccatttct
     6181 taagagtatt cgggtggcat ccagaatagt caatcggggc ctaatgaaat taaccaaatc
     6241 caccaaacta tcattcagat tccagtgaat tttgcccctg tttcctccca taatccccat
     6301 cagattcttt attcccaaag ttaatccagc aaagtcatga tgtttggcaa tggggacatt
     6361 aatcagcaaa tccgcctcca atacgggttt atatatctcc caacttttta tggcttcacc
     6421 ttgcggaaga ttcacctttg aaaaaccggc ttcaaccata taactaacct ctgcacctgc
     6481 ccggctggct gctttcatta ttccgcttct atcgtagcat ctgattgcgg cattacaact
     6541 gttatcaaaa actttcacct tcttagctcc tgcttccagg cacatcctca cgatctcagc
     6601 tacaacctcc ggattggtag tagccccttg ttctggagtt cgatcccaac ctatattggg
     6661 ttta
//
LOCUS       DASWLN010000192         3406 bp    DNA     linear   ENV 20-FEB-2024
DEFINITION  MAG TPA_asm: Candidatus Zixiibacteriota bacterium isolate
            SMAG_U2949 k141_882484, whole genome shotgun sequence.
ACCESSION   DASWLN010000192 DASWLN010000000
VERSION     DASWLN010000192.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37960594
            Sequence Read Archive: SRR10376091
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Candidatus Zixiibacteriota bacterium (soil metagenome)
  ORGANISM  Candidatus Zixiibacteriota bacterium
            Bacteria.
REFERENCE   1  (bases 1 to 3406)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 3406)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (26-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/12/2024 08:28:26
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 1,440
            CDSs (total)                      :: 1,416
            Genes (coding)                    :: 1,412
            CDSs (with protein)               :: 1,412
            Genes (RNA)                       :: 24
            rRNAs                             :: 1 (16S)
            partial rRNAs                     :: 1 (16S)
            tRNAs                             :: 21
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 4
            CDSs (without protein)            :: 4
            Pseudo Genes (ambiguous residues) :: 0 of 4
            Pseudo Genes (frameshifted)       :: 0 of 4
            Pseudo Genes (incomplete)         :: 3 of 4
            Pseudo Genes (internal stop)      :: 1 of 4
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..3406
                     /organism="Candidatus Zixiibacteriota bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_882484"
                     /isolate="SMAG_U2949"
                     /isolation_source="soil"
                     /db_xref="taxon:2053527"
                     /environmental_sample
                     /country="USA: Oregon"
                     /lat_lon="42.99 N 123.63 W"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            <1..639
                     /locus_tag="VGB16_07105"
     CDS             <1..639
                     /locus_tag="VGB16_07105"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEX9917492.1"
                     /translation="CPFASVRRCPRFYQSLSLLGGAGSTKIEPGEDKLLLKRWKKSDL
                     WPSTNEQATSMSGEPGEPSIYSNFCPEVAFERFGLFALNFARYADEIDLGLAHDRLGR
                     MNAPVTDWRWAWSSVAPMHYTECPLYSPLDLDVSRERSKGDDQGAPGNVPQAILRAIW
                     NWSPFWRKVALTIFIVLVLAFAIWVSLPDRKKERLLDALSNEAVQQTKPTGK"
     gene            983..2323
                     /locus_tag="VGB16_07110"
     CDS             983..2323
                     /locus_tag="VGB16_07110"
                     /inference="COORDINATES: protein motif:HMM:NF013704.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003677 - DNA binding [Evidence IEA];
                     GO_function: GO:0008170 - N-methyltransferase activity
                     [Evidence IEA];
                     GO_process: GO:0006306 - DNA methylation [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="DNA methyltransferase"
                     /protein_id="HEX9917493.1"
                     /translation="MDKKMLKIDKQIIDFFKHLKTNGNIGKLGTNSDNKNEAVHRWFS
                     FLPGFSHVFVKTTIEYFKPKADEPYAIFDPFMGSATTAVAGRSFGINVIGNESNQFLY
                     KIGKAKTHVVKNIESLTKIKQEVLAEARKKYKTQDISGEHSLLEKCYKKSNLKKLIAL
                     RNIYNSTLVCDEFKPYLFLTISALLPRCSNVGISIPYVSWSHERIPEEPLKLFGKIFD
                     NIKEDLSSIETQGDTETKIYLHDSRIENSKIKKDSIDLVFTSPPYLNNFDYGEALKVY
                     SYFWGFTKNWHEITEKVRKKSLTCATTHYKGLEYKSLLPKEILGKEFISDAPRTANII
                     AEKIKLITKAKSKKNREKSFDILTALYFKDMHNVLSEMHRVLKNDSLCFIAIGDSAPY
                     GVYVPTDEILGKLAVEIGFSKYSIDMLRTRGRKWLNLTHRHKVTLRESLLVLRK"
     gene            2325..3374
                     /locus_tag="VGB16_07115"
     CDS             2325..3374
                     /locus_tag="VGB16_07115"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEX9917494.1"
                     /translation="MQELPITQSIAKQKWSYIYAYNLRTLRDILVYCSLKGEIKEEQL
                     YDNMAKNIISPPTKQWVSSAIKRKERLRLEYIHAAEYLGLIKRKQKIITPDFEHFEVE
                     KKVVIEANKNRVFKPSYASPDLVDAEKWALTNILFDYGRVKDYLYWFLDFKKYKNVGG
                     LKLSDFKNEGKPIFLLGKIIAGKKGSDTIKRGADGKFWKIPESYIRLANSLFPGWFTE
                     LGLIDKVSVFPEFSKDKTLWHMFYPVKLSNDDFLNKDIGKLLEELFLKDTDKKSIWLP
                     QLIYVVALKYGCTTTAIKRALEKLYKEDYEHYYLERTSLSLMKRHRQYEGSYVKVDGF
                     YRSSLVLTRRSNKNG"
BASE COUNT         1070 a          637 c          768 g          931 t
ORIGIN      
        1 tgtccattcg cctccgtccg tcgctgtcca cgcttctacc agagtctctc gctccttgga
       61 ggtgctggaa gcacgaagat agagcctgga gaggataagc tgctcctgaa acggtggaag
      121 aagtcggatc tttggccaag caccaatgag caggcgacct ccatgagtgg ggagccgggc
      181 gaaccatcta tctactcgaa cttctgccca gaggttgcgt tcgagcgctt cggcctgttc
      241 gctttgaact tcgcccgtta cgccgatgag atcgatctgg gccttgcaca cgatcgtctc
      301 ggaagaatga acgcaccagt aaccgactgg cgctgggcat ggtcctctgt tgccccgatg
      361 cactacacag agtgcccgtt atactcccct ctcgatctgg atgtttcccg ggagagatca
      421 aaaggcgatg atcaaggtgc acctggtaac gttccccagg caattcttcg cgcgatatgg
      481 aactggagtc ccttctggag aaaagtggct ctgaccatat tcattgtcct tgtccttgct
      541 tttgccatct gggtatcact acccgaccga aagaaagagc gacttctgga tgccctttcc
      601 aatgaagcgg ttcagcaaac gaagccgaca ggcaagtagc gacacggctg cgccaagata
      661 tggagttaga attagggctt gtgtatatgc ctcacgtccc tacctggttc tcggaccctt
      721 ttccaagacc ttcttttaag ctttggaaaa aaggaagctt atgtttgaca tggaaatcgg
      781 agaggtatat tctgtgaacc aggaagtgcc agaacacggg gagacaccga aaacacattg
      841 gacaaattca tgacatctga taatgtgacc ctgttgtatt actctgatat actaaatcca
      901 actattccgt caggagagtt tttcaacagc ctgtgaagat ttgaccctca gtgtgccccc
      961 aattttctaa taggggttat gtttggataa aaagatgcta aaaattgaca aacaaattat
     1021 cgatttcttt aaacatttaa aaactaatgg gaatattgga aagctgggca cgaattccga
     1081 taacaaaaac gaagcagtgc atagatggtt ttcttttctt ccagggtttt ctcatgtttt
     1141 tgtcaaaaca accattgaat attttaagcc caaagccgat gaaccctatg ctatttttga
     1201 tccatttatg ggctcagcga ctactgctgt agctgggaga agctttggaa ttaatgtgat
     1261 tgggaatgag tcaaatcaat tcttgtataa gattggcaaa gctaaaaccc atgtggtaaa
     1321 aaacatagaa tcgttaacaa agattaaaca ggaagtttta gctgaggcgc gcaaaaagta
     1381 taaaacacag gatattagtg gagaacacag cttgttggaa aaatgttaca aaaagtccaa
     1441 tctcaaaaag ctgattgcat taaggaatat ttataattca actcttgtat gtgatgagtt
     1501 caagccgtat ctttttctta ctatttctgc acttctcccg agatgttcta atgtcggaat
     1561 aagcataccc tatgtttctt ggagccatga gcgtattcct gaagaaccct taaaactgtt
     1621 tggtaagatt tttgataata ttaaagagga tctttcaagt atcgaaacac aaggagatac
     1681 agaaacaaaa atttacttac acgatagtag aatagaaaac agtaaaataa agaaagatag
     1741 cattgatctt gtttttactt cgccacctta tttgaataat ttcgactacg gagaggcttt
     1801 aaaagtgtac tcatactttt ggggctttac gaaaaattgg cacgagataa cagaaaaggt
     1861 caggaagaag tcattaacct gcgctactac tcattataaa gggttagagt ataagtcttt
     1921 gcttcctaaa gaaatactag gaaaggagtt tatttcggat gctccgcgaa cagcaaatat
     1981 aattgcggaa aagataaagt taatcactaa ggctaaatca aagaagaaca gggagaaaag
     2041 ctttgacatc ctgacagcac tctattttaa ggatatgcac aatgttctaa gcgaaatgca
     2101 tagagttttg aaaaatgata gcctatgttt cattgctatt ggagactctg ctccctacgg
     2161 cgtttatgtc ccgactgatg agatcttggg caagttggct gtcgagattg gcttttcaaa
     2221 gtattcgata gacatgctaa ggacgcgagg gcgaaagtgg ctaaatctga cacacaggca
     2281 taaggttacc ttgcgagaaa gcctgttagt cttgaggaaa taaaatgcaa gaattaccta
     2341 taactcagtc tatagctaag cagaaatgga gctatatcta cgcatataat ctgagaacgt
     2401 taagggatat tcttgtttac tgttccctga aaggagagat aaaagaggag caattatatg
     2461 acaatatggc gaaaaatata atatcccccc ccacaaaaca atgggttagt tcagcgataa
     2521 aacgaaagga gcggctgagg ctggaatata ttcatgctgc tgagtatctg ggtttaatta
     2581 agcgaaaaca aaagattatt actcctgact ttgaacattt tgaagtggaa aagaaggtcg
     2641 tgatcgaggc gaacaaaaac cgcgtcttca agccttcata tgcttccccg gatttggttg
     2701 acgctgagaa gtgggcatta acaaacattc tttttgatta tggcagggtg aaagattatc
     2761 tatattggtt tttagacttt aaaaaatata aaaatgtggg gggcctcaag ctctctgatt
     2821 tcaagaacga aggaaagcca attttcctcc taggaaaaat tatagctggg aaaaagggca
     2881 gcgatacgat taagcggggg gcagatggta aattctggaa aatccctgag agttatataa
     2941 gattggccaa ttctcttttc cccggctggt ttactgaatt agggcttata gacaaggttt
     3001 cagtatttcc tgagtttagc aaagacaaaa ctctttggca catgttttat cccgtcaagc
     3061 tttcaaacga cgattttcta aataaggata taggaaagct attagaagaa ttattcctta
     3121 aggatacaga caaaaagagc atatggttgc cgcagttgat atatgtggta gcactaaaat
     3181 acggctgcac gaccactgca attaagaggg ctctggaaaa gctttataaa gaagattacg
     3241 aacattacta tttggagagg acttcattgt ctcttatgaa gaggcacagg caatatgaag
     3301 gcagttatgt taaggtcgat ggtttttata gatcgagttt agtattaacg aggaggtcta
     3361 ataaaaatgg ataatgtgaa tgaatttaca aaagaactgt tggaag
//
LOCUS       DASWLN010000193        10443 bp    DNA     linear   ENV 20-FEB-2024
DEFINITION  MAG TPA_asm: Candidatus Zixiibacteriota bacterium isolate
            SMAG_U2949 k141_1265602, whole genome shotgun sequence.
ACCESSION   DASWLN010000193 DASWLN010000000
VERSION     DASWLN010000193.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37960594
            Sequence Read Archive: SRR10376091
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Candidatus Zixiibacteriota bacterium (soil metagenome)
  ORGANISM  Candidatus Zixiibacteriota bacterium
            Bacteria.
REFERENCE   1  (bases 1 to 10443)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 10443)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (26-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/12/2024 08:28:26
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 1,440
            CDSs (total)                      :: 1,416
            Genes (coding)                    :: 1,412
            CDSs (with protein)               :: 1,412
            Genes (RNA)                       :: 24
            rRNAs                             :: 1 (16S)
            partial rRNAs                     :: 1 (16S)
            tRNAs                             :: 21
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 4
            CDSs (without protein)            :: 4
            Pseudo Genes (ambiguous residues) :: 0 of 4
            Pseudo Genes (frameshifted)       :: 0 of 4
            Pseudo Genes (incomplete)         :: 3 of 4
            Pseudo Genes (internal stop)      :: 1 of 4
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..10443
                     /organism="Candidatus Zixiibacteriota bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_1265602"
                     /isolate="SMAG_U2949"
                     /isolation_source="soil"
                     /db_xref="taxon:2053527"
                     /environmental_sample
                     /country="USA: Oregon"
                     /lat_lon="42.99 N 123.63 W"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            complement(86..562)
                     /gene="tadA"
                     /locus_tag="VGB16_07120"
     CDS             complement(86..562)
                     /gene="tadA"
                     /locus_tag="VGB16_07120"
                     /EC_number="3.5.4.33"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003670526.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008251 - tRNA-specific adenosine
                     deaminase activity [Evidence IEA];
                     GO_function: GO:0008270 - zinc ion binding [Evidence IEA];
                     GO_process: GO:0002100 - tRNA wobble adenosine to inosine
                     editing [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="tRNA adenosine(34) deaminase TadA"
                     /protein_id="HEX9917495.1"
                     /translation="MNQSEDQKWMRLALEEAMLAYKKDEIPIGAVIVHQGKVIGKGHN
                     QTESLKDATAHAEILAIGSASDFLKSWRLENCSIYVTVEPCIMCAGAIVLSRIKRLVF
                     GAYDMKNGACGSLYDIVSDKRLNHQAELTSGVLERECVEIVQNFFKKLRGKDGQSK"
     gene            complement(587..660)
                     /locus_tag="VGB16_07125"
     tRNA            complement(587..660)
                     /locus_tag="VGB16_07125"
                     /product="tRNA-Arg"
                     /inference="COORDINATES: profile:tRNAscan-SE:2.0.12"
                     /note="Derived by automated computational analysis using
                     gene prediction method: tRNAscan-SE."
                     /anticodon=(pos:complement(624..626),aa:Arg,seq:acg)
     gene            complement(795..1955)
                     /gene="wecB"
                     /locus_tag="VGB16_07130"
     CDS             complement(795..1955)
                     /gene="wecB"
                     /locus_tag="VGB16_07130"
                     /EC_number="5.1.3.14"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_012647201.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008761 - UDP-N-acetylglucosamine
                     2-epimerase activity [Evidence IEA];
                     GO_process: GO:0009103 - lipopolysaccharide biosynthetic
                     process [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="UDP-N-acetylglucosamine 2-epimerase
                     (non-hydrolyzing)"
                     /protein_id="HEX9917496.1"
                     /translation="MLKIIFIFGTRPEAIKVAPVIKELAKYPKKFKVINCITAQHRQM
                     LDQVLSLFEINVDYDLNIMKEDQDLFYLNSRLMPSLKKVLEREKPDLCLVQGDTTSAF
                     LGGLASYYQKIKVGHIEAGLRTHQKYSPFPEETNRHLLSVIADLHFAPTKKAKENLLR
                     ENIPSSKIFVTGNTVIDALLMIANKNYEFDNPILEKIDFTKKIILITAHRRESFGKPF
                     REMCYAFREIVNSYKDVELIYPVHLNPNVQKPVRNILKNVEGIHLIEPLDYKNFVLLM
                     KKSYLILTDSGGIQEEAPTFHKPVLVMREVTERPEAVEAGTAKVVGTQRKRIVEETSK
                     LLLSKKIYREMSSAKNPYGDGKSAKGIVRIISKELLHVSPPCPQGGTRQTSG"
     gene            complement(2064..4046)
                     /locus_tag="VGB16_07135"
     CDS             complement(2064..4046)
                     /locus_tag="VGB16_07135"
                     /inference="COORDINATES: protein motif:HMM:TIGR04183.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0031240 - external side of cell outer
                     membrane [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="T9SS type A sorting domain-containing protein"
                     /protein_id="HEX9917497.1"
                     /translation="MKRLILCLLVFSLVFSFLALQVLAKGKATEISTAVCNDQLRVQR
                     MANLRACFSNWGFFGSESRSLYETTGGCFMPNPSSTTQIAASFEFPKNSDLEYLYQGG
                     LWIGAIVNNETLTTVGVDGWQWINEIWPDGPSPLGDITEKSQKPGAPCSDASALADQE
                     MISVYSDTLIPGNMTDPFDKRFHKPINLKTTQHTYSWETPPYDNFVIVDLSVKNIGTD
                     TIHQTYLGLYMDTDIGHTTAPQGGSQDDITGFLKEYNGDTVNIGWAADNDGDPTSGVF
                     NYKSPTGVIGVKLLDFINPVPKISYNWWTSNAYDGDKDWGPWKLENAVRHQGLCPYGR
                     CDLNFPDNVKGTPGGDLSKYFIMSNGEIDYDQIYSYTDMTDSGWLSPSFYGLDFARGI
                     DTKFLFSFGPVEINPNDSIYATLAFLAGDSFHTDVNNYQNNLANSYSPSVYYSNLNFS
                     KLVAAGDTALSVYLNGYTTPPIGPPDSIWLANMTNDDLKIMWGSSSSPSLASFNIYRD
                     DVLLDTNIIDTFYVDTSATLSQTYDYAVSCVKTGGKEGKKSDQLEVTFTDVPWEKEST
                     VPLCYALEQNYPNPFNASTEIRYYLLEQSKVKITVYNTLGQKVRVLVDSKQDRGWKNA
                     IWNGKDQNDSPVASGIYFYRIATSKFQQVRKMVFLK"
     gene            complement(4173..4246)
                     /locus_tag="VGB16_07140"
     tRNA            complement(4173..4246)
                     /locus_tag="VGB16_07140"
                     /product="tRNA-Met"
                     /inference="COORDINATES: profile:tRNAscan-SE:2.0.12"
                     /note="Derived by automated computational analysis using
                     gene prediction method: tRNAscan-SE."
                     /anticodon=(pos:complement(4210..4212),aa:Met,seq:cat)
     gene            4438..5772
                     /locus_tag="VGB16_07145"
     CDS             4438..5772
                     /locus_tag="VGB16_07145"
                     /inference="COORDINATES: protein motif:HMM:NF025042.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="glycerate kinase"
                     /protein_id="HEX9917498.1"
                     /translation="MRSKQNILRKQGIKIFKAGIRAVNPEKLIKKFLRREQNKFYVGN
                     WVYDLSKIRKIIVVGAGKASAKMAKGVEEVLGRRVDSGIVITRYGYLSKLRRIRLIQA
                     GHPLPDKNGLRGTKKIIQLLKKTKENDLVICLISGGGSSLLVAPVPRVSLEDKRKTTD
                     LLLRCGANIAEINAIRKHLSRIKGGNLAQLAYPARVVSLILSDVPNDRMDVIASGPTV
                     ADKSNFYQCLKIIRKYDLSRKIPTSVDRYLKSSLGSKRNETPKPGSPFFKRVQNLIIG
                     SNLLALKGCKEKAERLRFKTHLISKPIVGDNLRAAINHTELAFRIKKEGKPISAPACI
                     ISGGETTVKVKGTGLGGRNQEFTLVCGKNAGKMKNFLILSASTDGTDGPTDAAGAFCD
                     ERVMKKVKVKGLDADEFLKNNDSYNFFKKVGGLVKIKPTGTNVMDIHLILVE"
     gene            5808..6737
                     /gene="pfkB"
                     /locus_tag="VGB16_07150"
     CDS             5808..6737
                     /gene="pfkB"
                     /locus_tag="VGB16_07150"
                     /EC_number="2.7.1.56"
                     /inference="COORDINATES: protein motif:HMM:TIGR03828.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008662 - 1-phosphofructokinase activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="1-phosphofructokinase"
                     /protein_id="HEX9917499.1"
                     /translation="MILTITANPTVDKGFHVKRIVKNDKLRAEHLRRDPGGGGINVSR
                     VIKRLGEETLACALAGGTTGELLAKLLVREKIPFRLTEIKDETRINFTVTEDNTGHQL
                     RFLMPGPKVKQSEWKRFVRDIVSIKPVPQLCVLSGSLPPGVPHDFYFRLIQVFKKKGI
                     KCFLDASGKPLRAGMKAGPHIVKPNLRELGQLTGKRMNSRELIRKATLGMLKCDCEMI
                     IISMGDKGAMLVTKEKSFYAIPPKVEPVSVVGAGDSMVAGMALKIAQGGSLSEILRFG
                     VAAGTAAVLSPGTELCTRKEFKKILPKVKISFL"
     gene            6828..7223
                     /locus_tag="VGB16_07155"
     CDS             6828..7223
                     /locus_tag="VGB16_07155"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_018124052.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="class II SORL domain-containing protein"
                     /protein_id="HEX9917500.1"
                     /translation="MQNLSEHIQRADWKKEKHVPVIECPSTAKAGENFEIKVSPGKEI
                     AHPNTTEHHIRWISVFFHPEGEKFTYQLGYFEFSAHGESTEGPNKGPVYIDHKITAWF
                     RTNKPGTIYALALCNIHGLWEGSKEITVE"
     gene            7239..7427
                     /locus_tag="VGB16_07160"
     CDS             7239..7427
                     /locus_tag="VGB16_07160"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_007220106.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005506 - iron ion binding [Evidence IEA];
                     GO_function: GO:0009055 - electron transfer activity
                     [Evidence IEA];
                     GO_function: GO:0051539 - 4 iron, 4 sulfur cluster binding
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="ferredoxin"
                     /protein_id="HEX9917501.1"
                     /translation="MKVRVDQELCTGNEICTQVCPELFEMHGDKAVARMTDVPEGLEE
                     CCSQAASSCPSEAILIEE"
     gene            7845..7916
                     /locus_tag="VGB16_07165"
     tRNA            7845..7916
                     /locus_tag="VGB16_07165"
                     /product="tRNA-Gly"
                     /inference="COORDINATES: profile:tRNAscan-SE:2.0.12"
                     /note="Derived by automated computational analysis using
                     gene prediction method: tRNAscan-SE."
                     /anticodon=(pos:7877..7879,aa:Gly,seq:ccc)
     gene            8091..9416
                     /locus_tag="VGB16_07170"
     CDS             8091..9416
                     /locus_tag="VGB16_07170"
                     /inference="COORDINATES: protein motif:HMM:NF016354.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF512 domain-containing protein"
                     /protein_id="HEX9917502.1"
                     /translation="MIRVKKVRPNSLAKIYGILPGDKLQEINSRPIRDIIDFYFYSAD
                     ENLKLKFRRKKGKSEIIEIKRGINQDLGLVFHDDRIRGCGNKCIFCFVDQLPKGLRKP
                     LYFKDEDLRLSFLKGNFITLTNTSKNDINRIKQQRLSPLYISVHTVNKDLRKKMLGNL
                     RIPSIVPLIGELVKGKIELHIQIVLCPGINDGKYLRESIEVLSSFYPYVKSIAIVPVG
                     LTKCREGLFTIKPVTKSTASKIIKLVGKYQKKFQKKHRSNLVYLADEFFLLAGLDIPK
                     AKYYDGFRQIENGVGLLRKLIDDFQKDKRKLPSSLKRKISLTLVTGRLASAYIKKMQR
                     ELNRIENLKVKVIAVENNFLGRSITVSGLLSGRDIIKVLKKEKKLGKVTILPPDCVNY
                     AGKFLDDLKVKDIEKGIKRKVLLGSYDMVDTVLKAVKCSFKNKNICPSR"
     gene            9401..>10443
                     /gene="der"
                     /locus_tag="VGB16_07175"
     CDS             9401..>10443
                     /gene="der"
                     /locus_tag="VGB16_07175"
                     /EC_number="3.6.5.-"
                     /inference="COORDINATES: protein motif:HMM:TIGR03594.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0005737 - cytoplasm [Evidence IEA];
                     GO_function: GO:0003924 - GTPase activity [Evidence IEA];
                     GO_function: GO:0005525 - GTP binding [Evidence IEA];
                     GO_function: GO:0043022 - ribosome binding [Evidence IEA];
                     GO_process: GO:0042254 - ribosome biogenesis [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="ribosome biogenesis GTPase Der"
                     /protein_id="HEX9917503.1"
                     /translation="MSKPIVAIIGRPNVGKSTLFNRLIKKRHAVVNGEPGVTRDRNYH
                     LASWSGKSFYLVDTGGLVPSAKNEIEKLVKAQAEIAVDEADLVLFLIDTKVGAQEIDL
                     EIADKLRKRGKNLILVANKVDSEKDELEIYALNRLGLGEPFSISAQNGRNIGDLLDRI
                     IDFVPEKIEEEEERESIKVAVVGRTNVGKSSFVNALLGENKLIVSSQPGTTRDSIDTQ
                     FKIEDRLFTLIDTAGLKKKSKVKDGIEYYTSLRTLRSIERCDVALLLVEAQIGLLKQD
                     IKIAQEIADQWKGIVLVVNKWDLVEKDSKTADIYTKQIKEVAPFMDYAPIIYISALTG
                     QRVYPVLKLVGEV"
BASE COUNT         3209 a         2042 c         2331 g         2861 t
ORIGIN      
        1 agcgagcaag acggctgaaa caaaaaaatc taagactatt ccccagtagg tcgccttcat
       61 atggacttta tcaatatcca atggctcatt tggactggcc atcttttccc cttaacttct
      121 taaagaaatt ttgaactatc tctacacatt ctctttcgag aacaccactg gtaagttcgg
      181 cctgatgatt tagtctcttg tccgaaacaa tatcatacaa agacccgcaa gcaccatttt
      241 tcatatcgta tgcaccaaag accaatctct ttattctgga gagaacgatt gcgccagcgc
      301 acattataca gggttcaacc gtaacataaa ttgaacaatt ctccaatctc caggatttca
      361 ggaaatcgga agcggagccg atcgccaaaa tctcagcatg ggctgtggca tcttttagac
      421 tttcggtctg gttatgccct tttccaatga cctttccctg atgaacgatg accgccccaa
      481 tagggatctc gtcctttttg taggcgagca tcgcttcttc aagtgcaagc cgcatccact
      541 tttggtcttc agattggttc aaaatagtag ggaaaatatt aatatatacg cccaggagga
      601 tttgaacccc caaccctctg atccgtagtc agatgctcta tccaattgag ctatgggcgc
      661 attaagtttt cagtttaaat ataaatctcc agtcttattt gtcaatatat ttcaaaatgc
      721 aattattccg ataaacttga tagttacagc agaacaattg tagagataag aaaacggtaa
      781 gccccacatt gccttcaacc tgaggtttgc cgggtcccac cttgagggca aggtggggat
      841 acgtgaagaa gttccttgga gattattctc acaattcctt ttgcagactt gccatctccg
      901 taggggttct tagcggatga catctctcgg tagattttct tggacaaaag aagtttggag
      961 gtttcctcaa caattctttt tctttgtgtt cccactactt tggctgttcc tgcctcaacc
     1021 gcttcgggcc tttcagttac ctctcgcatc actaaaaccg gcttatgaaa agttggcgct
     1081 tcttcctgaa ttccaccaga gtcagtcaat ataagataag atttcttcat caatagaacg
     1141 aaattcttat aatctaatgg ctcgatgaga tgaatccctt caacattctt taagatattc
     1201 ctaaccggct tctgcacatt cggatttaag tgaaccggat agattagttc cacgtcttta
     1261 tacgagttca caatctcccg gaaagcataa cacatctccc taaatggctt gccaaaactt
     1321 tctcgacggt gagcagtaat caaaattatt tttttggtga aatctatttt ctctaaaatt
     1381 ggattatcaa attcgtaatt tttgttggca atcataagca aagcatcaat aaccgtattt
     1441 ccggtaacaa atattttact gctcgggata ttctcccgca ataagttttc tttcgctttt
     1501 ttggttgggg caaaatgcaa gtccgcaatg accgacaaaa gatgacgatt ggtttcttca
     1561 gggaaagggg agtatttttg atgagttctc aagccagctt cgatatgccc cacttttatt
     1621 ttttgatagt aagatgccag cccacccaaa aatgcagaag tggtgtcccc ttgaaccaaa
     1681 cacaaatccg gtttttctct ttccaaaacc tttttgagag aaggcattaa tctggagttt
     1741 aaatagaaga ggtcctggtc ttccttcatt atgttcagat cgtaatccac gttaatctca
     1801 aacaaggaaa ggacctggtc gagcatctgc ctgtgctggg cagtgatgca gtttatgacc
     1861 ttgaactttt tgggatattt tgccagctcc tttataaccg gagcaacttt tatggcttcg
     1921 ggtcgcgtgc cgaatatgaa aataatcttt agcacttacc tccttattag tttaacaaaa
     1981 attatactac aaattcatat ttatgtcaaa caaaaagacc gcccaaaagc aggcggtctt
     2041 ttaagttacc ttgttagatt cgattatttc aagaaaacca tttttctgac ctgttggaat
     2101 ttgctggtgg caatacggta gaaatatatt ccgctggcaa ctggactgtc attctggtct
     2161 tttccgttcc atatagcatt tttccagcca cgatcttgtt tactgtccac caagactcta
     2221 accttttggc caagggtatt gtagaccgtt atcttaacct tgctttgctc cagaagataa
     2281 tatcttatct cagttgaagc gttgaaagga ttgggatagt tctgctcaag tgcataacac
     2341 agaggtacag tcgattcctt ctcccacggc acatccgtga aagtaacctc gagctggtct
     2401 gacttctttc cctctttgcc acctgtttta acacaggaga cagcatagtc atatgtctgg
     2461 ctcaaggttg cgctggtatc aacatagaag gtgtcaatta tgttggtatc caaaagaaca
     2521 tcatctcgat agatattgaa acttgccaga gaaggtgagc tggacgagcc ccacataatt
     2581 tttaagtcgt cgttagtcat attggctaac cagatgctat ccggtggccc aatgggcgga
     2641 gtagtatatc catttaaata aactgaaagg gcagtatcac ctgcagctac caacttgctg
     2701 aaatttaggt tgctgtaata gacactgggg ctatagctgt tagcaagatt gttctggtag
     2761 ttattaacgt cagtatggaa actatcacca gctaagaaag ccaaagtggc atagattgag
     2821 tcgttcggat ttatctcaac cggaccaaac gaaaagagaa atttagtatc tattccacga
     2881 gcaaaatcta atccgtaaaa actcggggat agccaacccg aatcagtcat gtccgtgtag
     2941 ctataaatct ggtcgtagtc tatctcgccg ttcgacatta tgaaatattt agataaatcc
     3001 ccgcccggtg tgcctttcac gttatctggg aaattgagat cacaccttcc atatggacaa
     3061 agcccttgat gtctaactgc gttttctaac ttccatggtc cccagtcctt atctccatcg
     3121 tacgcattgg aagtccacca gttataagaa atcttaggaa caggattgat gaaatccaga
     3181 agctttacac ctatgactcc agtagggctt ttgtagttga aaactccgct agtagggtct
     3241 ccgtcattat cagccgccca gccaatatta actgtgtctc cattatattc cttaaggaag
     3301 ccggtgatgt cgtcttggga cccaccttga ggtgcagttg tatgtccgat gtcagtatcc
     3361 atgtaaagcc ccaagtaagt ctgatgaatg gtatcagttc cgatattctt tacggataag
     3421 tccacaatca caaaattgtc ataaggtggg gtctcccaag aataggtgtg ctgagttgtt
     3481 ttcaaattta tgggtttgtg gaagcgctta tcaaaagggt ctgtcatgtt tccaggtatg
     3541 agggtatcag aatatactga tatcatctct tggtcagcta aggcactggc atcactgcag
     3601 ggtgcaccgg gtttttgtga tttttcagtt atgtcgccca agggagatgg tccatcaggc
     3661 cagatttcat ttatccattg ccaaccatcg accccaaccg tagtcagagt ttcattattg
     3721 acgattgcgc ctatccatag gcctccttga tacagatatt ctaagtctga attcttaggg
     3781 aattcaaagg aggcagctat ttgagttgtt gaactcgggt tgggcataaa gcatccccca
     3841 gtggtttcat agagacttct gctctcgctt ccaaaaaatc cccaattact aaagcaagct
     3901 cgaagattag ccattctttg cactcttaac tggtcattgc agacagcagt tgaaatttca
     3961 gttgctttgc ctttggcaag aacctgtaaa gccaaaaaag aaaagaccaa ggaaaacaca
     4021 agcagacaca atatcaatct tttcattata gcctcccttc tttgtttaga ttgttatctt
     4081 ttataactcc ttagtatttt tcggagggac tcttaaacgg atagatgcaa gtaattagta
     4141 acaaatatat atatatattg tcaagctaaa aatagcggtg cagggactcg aaccccggac
     4201 acgcggatta tgattccgct gctctaacca actgagctac accgccatag ttttttactt
     4261 tataataaaa acttaaacga ttgtcaagaa gagattacac caaactcatt tgcttttctt
     4321 tttgacaaaa gcggtttgaa attcaattct tcacctcttg aatgattatt ttcttgatta
     4381 tcaaacagaa atgctttata taattcttta gagacaaaac agaacaacaa acagaatatg
     4441 agatcaaaac agaatattct gcgaaaacag ggcataaaga tattcaaagc agggataaga
     4501 gcagtcaatc ctgaaaagct aatcaagaaa ttcctaagga gagaacaaaa taagttttac
     4561 gtagggaatt gggtttatga cttaagcaag ataagaaaaa ttatagtcgt gggtgcgggt
     4621 aaagcctcag ctaagatggc aaaaggggtg gaggaagttt taggtagaag ggtagattct
     4681 ggcatagtca taaccagata tggttatctc tccaaacttc gccgcataag actgattcag
     4741 gcaggacatc cacttccgga taaaaatggc ttaagaggaa ctaagaagat tattcaactt
     4801 ttaaaaaaga caaaggaaaa cgatttagtc atctgcttga tttcaggagg tggctcatct
     4861 cttttggttg ccccggttcc gagggtaagc ttggaggata aaagaaagac aacggattta
     4921 cttcttagat gcggtgcgaa tatagctgaa ataaatgcta ttcgcaaaca tctttctcga
     4981 ataaagggag gaaacttagc ccaattagct tatccggcaa gagtggtttc ccttatactt
     5041 tcagatgtgc caaatgatag aatggatgtg attgcctctg gtcctacggt tgcggacaag
     5101 agcaattttt atcaatgcct aaagataata aggaaatatg acctatccag aaaaattcct
     5161 acgtcagttg atcgatacct gaaaagtagt ctgggaagca agagaaatga gacccccaag
     5221 ccgggctccc cgtttttcaa aagagtacaa aatctaatca tcgggtcaaa tcttttggct
     5281 ctcaaaggat gcaaggaaaa agcagaacga ttgagattta aaactcatct catatccaaa
     5341 ccgatagtcg gggataacct cagagccgcc ataaatcata cagagctggc ttttcgtata
     5401 aaaaaagaag gaaagcccat ctctgctcca gcgtgcataa tttccggagg tgaaaccacg
     5461 gttaaagtta agggaactgg gttaggtggt agaaatcagg agttcacctt ggtctgtggg
     5521 aagaacgcag gaaagatgaa gaattttctt atcttaagtg ccagcaccga tggaactgat
     5581 ggtcctacgg atgcagcagg ggcattctgc gatgagaggg ttatgaaaaa agtcaaagtc
     5641 aaaggtctgg atgcagatga attcttaaag aataatgact cctacaattt cttcaagaag
     5701 gttggggggt tagtcaagat aaaaccaacc ggcacaaacg tgatggatat tcatttgata
     5761 ctggttgaat aagaatagat tgaaaatatt ttagaggtta gaaatatatg atacttacca
     5821 ttactgccaa tccaactgtg gataagggat ttcatgtaaa gcggatcgtc aaaaacgaca
     5881 aattgcgagc tgagcatcta agaagagatc cgggcggagg tggaataaac gtatcacggg
     5941 tgattaaacg cttgggagaa gagaccctgg cttgtgcctt ggctggtgga actacaggtg
     6001 aattgcttgc caagttactc gtaagagaga aaattccttt tcgtctcact gaaatcaaag
     6061 atgagactag aataaatttc acagtgacgg aggataacac tggccaccag cttcgtttcc
     6121 ttatgcctgg tcccaaagtg aagcagagcg aatggaaacg atttgtacga gacattgtct
     6181 ccataaaacc ggtcccacag ctttgtgtgt taagtggaag tttgcccccg ggcgttccac
     6241 atgattttta ctttcgcctg attcaagtat tcaaaaagaa aggaattaag tgctttttgg
     6301 atgccagtgg caagccgctc agggctggta tgaaagccgg tccgcatatt gtcaaaccta
     6361 acctgagaga gttgggtcag ctgacaggaa aaagaatgaa ttcgagagag cttatccgca
     6421 aggcaacact tggaatgttg aaatgtgatt gcgaaatgat aataatctct atgggagaca
     6481 aaggggcaat gttggtaact aaagaaaaat cattttatgc tattcctcct aaggtcgagc
     6541 cagttagcgt agtaggagca ggagattcta tggttgctgg tatggctttg aagatagccc
     6601 aaggcggttc cctgtcagag attttgcgtt ttggagttgc ggcgggaaca gccgcagtgc
     6661 tgtctccggg aacagaattg tgcacaagaa aagaattcaa aaagatcttg cctaaagtaa
     6721 aaattagttt cttatgaaac atattggcaa aggacaaacc ctatggcgcg tatttaagtt
     6781 acaagaaacg tttttgaaca aattaaagta tggaaagggg tgacgaaatg caaaacttaa
     6841 gtgagcatat ccaaagggct gattggaaaa aggagaagca tgtgccagtg atagaatgcc
     6901 cctcaacagc gaaagccgga gagaatttcg aaataaaagt aagtcccgga aaggagatag
     6961 cacatcccaa tacaaccgag catcatatac gctggatatc cgttttcttt catccggagg
     7021 gagagaagtt cacctatcaa cttggatact tcgaattctc cgcacatggc gaatcaactg
     7081 agggacccaa taaggggccg gtctacattg accacaagat aacggcttgg tttagaacca
     7141 acaaacccgg cacgatttac gctttggcgc tatgtaacat tcatgggttg tgggagggaa
     7201 gcaaagagat aaccgttgaa tgaaaagaag gggccaagat gaaagttaga gttgaccaag
     7261 agttgtgcac cgggaatgag atttgcacgc aggtttgtcc agaacttttt gagatgcatg
     7321 gtgataaggc agttgccaga atgactgatg ttccagaggg cctggaggaa tgttgcagcc
     7381 aggcagccag ctcctgtcca tctgaagcaa tcttgattga ggaatagaga ggaatcgcca
     7441 gattcccctg tattacagta cgctagggaa gatttaggca gcttccccaa aaacaagcct
     7501 tagcttcctg tgatctcgtg tgaatcagca gaataagtaa acccaaatct caaaaaggat
     7561 tctgctcttg aactccaagt cagattggtt gatttagaga ttgtttcctc tcccccgcgg
     7621 gagagaggaa gaattaaccc caagctacgt ctcaagaccc agaattcggg gaattcgcac
     7681 aaagatacca ttttatccct gtggtatgaa ccgtaatggt ttatacctct gggcagaatc
     7741 ctagtgttgc cttggatcga aaaacgggga aagtccacgg gaagaaatcc cttgacaatt
     7801 cggcttcaag ttattttttt agaaatcaaa taaggcattt tcaagcgggt gtaattcagc
     7861 ggtagaatgc aagcttccca agcttgacgt cgagggttca actcccttcg cccgcttttt
     7921 actcgcccct gccagagagt ctcgctcgcc tctcggcgct cgcctctcgg cgaagccgag
     7981 ggaagccgag ggaagccgag ggcctttggc ggaaatagag aggcgagcct tttatatttg
     8041 ggaagccccg tgatttatca cggggagact tcactatttt tatttcctag atgattagag
     8101 taaaaaaagt aagacccaat agcttggcga agatatatgg cattctgcca ggggacaagt
     8161 tacaagaaat aaactcccgc cccataagag atataattga tttctacttc tattctgcgg
     8221 acgagaacct taagcttaag tttagacgca aaaaaggaaa atccgagata attgaaataa
     8281 agaggggcat aaaccaagac ctggggctgg tttttcacga cgacagaatc agggggtgtg
     8341 gcaataagtg tattttctgt tttgttgacc aactccccaa aggattaaga aaaccactct
     8401 attttaaaga tgaggacctc cgtctatctt ttttaaaagg aaatttcata accttaacta
     8461 acacttcaaa gaatgacatt aaccgcatta aacaacaaag acttagccca ctttatattt
     8521 cagtccacac ggtcaacaag gacttacgta aaaagatgtt gggcaactta aggataccct
     8581 cgatagttcc cctgatagga gaattagtta aaggcaaaat agagcttcac atccagatcg
     8641 ttttatgccc aggaataaac gatggtaagt atttaaggga atcgattgaa gttttgtcca
     8701 gtttttatcc ttatgtgaaa tccatagcca ttgtgccggt gggcttaact aaatgcagag
     8761 aaggactgtt tacgattaag ccggtcacta aatcaacagc ttcaaagatc ataaaattag
     8821 ttgggaaata ccaaaagaag tttcaaaaaa aacatagaag caatctggtc tatctggcag
     8881 atgagttttt tcttttggct gggcttgaca ttccaaaagc taagtattat gatggattcc
     8941 ggcaaataga gaatggagtc gggcttttaa gaaagctcat agatgatttc caaaaagata
     9001 aaagaaaact cccttcaagc ttgaaaagaa agattagttt gactttggtt acaggtaggt
     9061 tggcttcagc ctatatcaaa aagatgcaga gggaactgaa tcgaatagaa aacctgaagg
     9121 tcaaagtcat agcagtagaa aataactttt tgggcagaag tattacagta tcgggtttgt
     9181 taagtggcag agacataatt aaggttttaa aaaaagaaaa aaagttagga aaagtaacta
     9241 ttttgccacc ggattgcgta aattacgcag gaaagttttt ggatgattta aaagtcaaag
     9301 acattgaaaa gggcatcaaa cgtaaggttc ttttaggttc atacgatatg gttgatactg
     9361 ttcttaaagc cgtaaaatgc agttttaaaa ataaaaacat atgtccaagc cgatagttgc
     9421 tataattggt cgtcccaacg ttgggaaatc aactctgttc aaccgcctga ttaagaagcg
     9481 tcacgcagtg gtgaatggtg agccgggagt aacgcgggac aggaactatc atctggccag
     9541 ctggtctgga aagagttttt atttggttga tactgggggt ctggttcctt ctgccaagaa
     9601 tgagattgag aaattggtga aagcccaggc ggagattgcc gtagatgagg cagatttggt
     9661 cttattcttg attgacacta aagtgggtgc acaggagata gatttagaga tagcagacaa
     9721 gctaagaaaa agaggtaaga acttgatact ggtagccaat aaggtggata gtgaaaaaga
     9781 tgagttagag atttatgctc tgaatagatt gggtttgggt gagccatttt ccatctcagc
     9841 tcaaaatggt agaaacatag gagacctgtt ggaccgcata atagattttg tgcctgaaaa
     9901 aatagaggag gaagaagaga gggagtcaat taaagtggcg gtggtgggaa gaactaatgt
     9961 gggaaagtca tcttttgtca atgccctttt gggagaaaat aaattaattg tctcttccca
    10021 gccgggaaca actcgcgact cgattgatac ccagttcaaa atcgaagaca gactttttac
    10081 cctgatagac accgcaggtc tgaagaagaa atcaaaggtc aaggatggaa tagaatatta
    10141 tacttccctg cgcaccttga ggagcataga acgttgtgat gtggcgttgt tattggttga
    10201 agcgcaaatc ggattgctca agcaggatat aaaaatagct caagagatag ctgaccaatg
    10261 gaaagggata gtcttggtgg tgaacaagtg ggacttggtg gaaaaggata gtaagaccgc
    10321 agatatttat accaaacaga taaaagaagt agctccattc atggattatg ctccgatcat
    10381 atatatctcc gccttgaccg ggcagagggt ttatccggtt ttgaaactgg tgggggaggt
    10441 tca
//
LOCUS       DASWLN010000194         3796 bp    DNA     linear   ENV 20-FEB-2024
DEFINITION  MAG TPA_asm: Candidatus Zixiibacteriota bacterium isolate
            SMAG_U2949 k141_1265846, whole genome shotgun sequence.
ACCESSION   DASWLN010000194 DASWLN010000000
VERSION     DASWLN010000194.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37960594
            Sequence Read Archive: SRR10376091
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Candidatus Zixiibacteriota bacterium (soil metagenome)
  ORGANISM  Candidatus Zixiibacteriota bacterium
            Bacteria.
REFERENCE   1  (bases 1 to 3796)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 3796)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (26-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/12/2024 08:28:26
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 1,440
            CDSs (total)                      :: 1,416
            Genes (coding)                    :: 1,412
            CDSs (with protein)               :: 1,412
            Genes (RNA)                       :: 24
            rRNAs                             :: 1 (16S)
            partial rRNAs                     :: 1 (16S)
            tRNAs                             :: 21
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 4
            CDSs (without protein)            :: 4
            Pseudo Genes (ambiguous residues) :: 0 of 4
            Pseudo Genes (frameshifted)       :: 0 of 4
            Pseudo Genes (incomplete)         :: 3 of 4
            Pseudo Genes (internal stop)      :: 1 of 4
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..3796
                     /organism="Candidatus Zixiibacteriota bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_1265846"
                     /isolate="SMAG_U2949"
                     /isolation_source="soil"
                     /db_xref="taxon:2053527"
                     /environmental_sample
                     /country="USA: Oregon"
                     /lat_lon="42.99 N 123.63 W"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            <1..886
                     /locus_tag="VGB16_07180"
     CDS             <1..886
                     /locus_tag="VGB16_07180"
                     /inference="COORDINATES: protein motif:HMM:NF025030.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=2
                     /transl_table=11
                     /product="HEAT repeat domain-containing protein"
                     /protein_id="HEX9917504.1"
                     /translation="EPQDSIQIDSTQLRLDSLFALASGGIVGYGQSADSSKKALISMK
                     EEVVPYMIDKLGSKDVRETNTGIDILKGIGEPAVPYLIDALADTNNGKVAIVLRTLGE
                     MKAAGAVMPLVNMVKHEDFRIRSSACTALGLIGDTSAAHHLIPALNDTVNLVRKSAAF
                     ALGEARSPKSIPYLVNALSDEFYGTRFAASNSLVKFGVVTTKSLLPLLNAQDLNLQCL
                     AIQTLGKIKDKKALDPLLVNLTSPDWAVRGFAVEALGNIGNGKTTKAIKDLEKTETHP
                     FVKRKIEEALEKTAKTIE"
     gene            888..1463
                     /locus_tag="VGB16_07185"
     CDS             888..1463
                     /locus_tag="VGB16_07185"
                     /EC_number="3.2.2.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_010076035.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003905 - alkylbase DNA N-glycosylase
                     activity [Evidence IEA];
                     GO_process: GO:0006284 - base-excision repair [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="DNA-3-methyladenine glycosylase"
                     /protein_id="HEX9917505.1"
                     /translation="MKRRLRRCFYNRATLKVARELLGKYLVLKRDGKFLSGKIVETEA
                     YIGRGDKASHASRGKTKRNEVMFGAPGYAYIYFTYGMYHCLNFVTEKENFPAAVLIRA
                     LQPKDGVEIMKKNRKTDTLENLTNGPGKVCMALGLDRRMNGIDLCSDILYVEDRNERK
                     GKFISTSRVGINVGKDKKWRFHIKDNKFVSR"
     gene            1729..2385
                     /locus_tag="VGB16_07190"
     CDS             1729..2385
                     /locus_tag="VGB16_07190"
                     /inference="COORDINATES: protein motif:HMM:NF014244.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="site-2 protease family protein"
                     /protein_id="HEX9917506.1"
                     /translation="MGLEYYLLITPPLFFAFTVHEYAHAWAAYKKGDNTAKLAGRLTL
                     NPLAHIDILGTILLYLYGFGWAKPVPVNPYNFRNPRKDDMLVSFAGPVSNLFSAFIYG
                     ITFRIFTGYSMGSGSEASLGNVLFTMVVFGLRLNIILAFFNLIPISPLDGSHILMGLV
                     PLKYTPWVARLQRIGPFFLLGIVLMDYVLGTRILWGFFGPFIAFFGTLFAGPQFIRSM
                     "
     gene            2389..3153
                     /locus_tag="VGB16_07195"
     CDS             2389..3153
                     /locus_tag="VGB16_07195"
                     /inference="COORDINATES: protein motif:HMM:NF025490.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF4292 domain-containing protein"
                     /protein_id="HEX9917507.1"
                     /translation="MLKKLPELILLAIFLSLLSCFPKGIPLEERTPAKVLDKLLQQEN
                     KLHSSANLISFRAKNRNESISSNLELYWTEPDTFAFYFQSIFGSNTVRGRLIEDTLEL
                     YFADENRYFKGDSVELEEKDFLTKNVKEILNLAVGKYGLEKEKLTDSYPRGDKVVYRF
                     EDERKVWELWVDAKKNRVEKGTFFLKDNKASYNLNFKSYFENRGFEFPRLIEISSSSG
                     DEYIKLKFIERKVNYSIPPNRFDLKIPQDAVQTDSF"
     gene            3478..>3796
                     /locus_tag="VGB16_07200"
     CDS             3478..>3796
                     /locus_tag="VGB16_07200"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEX9917508.1"
                     /translation="MYRRVLKYIKPYWKHLVGSILATILFAIASGALVWLVGPLMGTL
                     FSLEGYSQVNLNQALDSSKSVEKELTPTLTSLKETLKNSANRLIIGKTKYDTLKKLCI
                     IIIF"
BASE COUNT         1138 a          712 c          903 g         1043 t
ORIGIN      
        1 tgaaccccaa gacagtatcc agatagactc aacccagcta agattagaca gtctttttgc
       61 ccttgcctcg ggtgggattg tgggttacgg acaatcagct gattcctcga agaaagctct
      121 tataagtatg aaggaggagg ttgttcccta catgattgat aaattgggga gcaaagacgt
      181 aagagaaaca aataccggaa ttgatatcct caaaggaatt ggagagccgg cagtgccata
      241 tttgatagat gccttggcag acaccaacaa cggcaaggtg gctatagtgc ttcgcacttt
      301 aggagagatg aaagctgctg gagcggttat gccgcttgta aatatggtaa aacatgagga
      361 cttcaggata agaagtagtg cttgcaccgc tttgggttta attggagata catccgctgc
      421 tcatcattta atacctgccc taaatgatac agtcaatctg gttagaaaga gcgccgcttt
      481 cgcattaggt gaagccagaa gccctaaaag cataccatat ttggtgaatg ccttgtcaga
      541 tgagttctat ggaacgagat tcgcggctag caattctctg gtcaaattcg gagtggtaac
      601 aactaaatcc ttacttccgc ttctgaatgc ccaagacttg aatctgcagt gcctggcgat
      661 tcagactttg ggaaagataa aggataaaaa agcccttgac ccgcttttgg tgaacttaac
      721 cagcccggat tgggcagtca ggggatttgc ggttgaagct ttagggaata ttggaaatgg
      781 aaaaacaacc aaggcgataa aggatttaga aaagacagaa actcacccgt ttgtgaaaag
      841 gaaaatagaa gaggcattgg aaaagacagc caagaccatt gagtagtatg aaaagaaggt
      901 tacgtagatg tttttacaat cgtgccactt tgaaagtggc acgagaactt ttggggaaat
      961 atctcgtatt gaaaagagat gggaagtttc tttccggcaa gatagttgaa accgaggcat
     1021 acattggacg gggtgataaa gccagtcatg cttctcgggg aaaaaccaag agaaatgagg
     1081 ttatgttcgg tgcaccagga tatgcttata tttacttcac ctacggaatg tatcattgtc
     1141 taaatttcgt cacggaaaaa gaaaactttc cagctgcggt tttaattcgc gccttgcagc
     1201 cgaaagatgg ggttgaaatt atgaaaaaga acagaaaaac agatacgctc gagaacctga
     1261 ccaacggtcc gggcaaggtc tgtatggctt tgggcttgga tagaagaatg aacgggattg
     1321 atttatgttc ggatattctg tatgtggaag atagaaatga gaggaaaggg aaattcatct
     1381 ccacttcacg agttggaata aatgtcggga aggataagaa gtggaggttt catataaaag
     1441 acaacaaatt cgtatcaaga taatcaggta aaaaatggga tgggataaat tcccatccct
     1501 acgaaaaggc atttataatg taagggcggg tctttagacc cgcccgaacg gactaaaaaa
     1561 gtatggcaga gtggtcgggc aaggtgcccg acctacgaaa aatcaatgac ttatctcaag
     1621 acacagaatt cgtgcattcc cgtaggcagg gctcccagcc ctgcaaaagg ggtttttcag
     1681 taatctcata aattgggcaa ctacagtgaa gataatttag aggcttgaat gggacttgaa
     1741 tattatttgc tcataactcc tcctttgttt tttgccttca cggtgcacga atatgctcat
     1801 gcctgggcag cctataaaaa gggagacaat actgccaagt tagcagggag attgacttta
     1861 aaccccttag cgcatatcga tattttagga acaatcttac tttatctata tggttttggc
     1921 tgggctaagc cggttccggt taatccttac aatttcagaa acccgagaaa ggacgatatg
     1981 ctggtctcct ttgctgggcc ggtgtcaaat ttattttccg ctttcattta cggcataaca
     2041 tttcgcatat tcacaggtta tagcatgggg agtgggtcag aagcatcttt gggaaatgtg
     2101 cttttcacta tggtggtttt cggattaaga ttaaatatca ttttggcttt tttcaatttg
     2161 attcccataa gtcccctgga cggctcgcat atcctgatgg gattagttcc cctaaaatat
     2221 accccttggg tggcaaggct ccagagaatt ggtccatttt ttctcttggg aattgtgcta
     2281 atggattatg ttttgggaac aagaatcttg tggggatttt ttggaccgtt tattgctttc
     2341 tttggaactc tttttgccgg accccaattc attcgctcta tgtaatctat gctcaagaaa
     2401 ttaccagaat taattctgct cgctatattc ttatccctgc tgagttgttt ccctaaggga
     2461 attcccctgg aggagcggac cccggcaaag gttttggata agctcctgca acaggaaaat
     2521 aagttgcaca gctcggcaaa tctgataagc tttagggcga aaaaccgcaa cgagagcata
     2581 tcctccaatc tggagcttta ttggacagag ccggacactt tcgcctttta ttttcagtct
     2641 atctttgggt cgaatactgt tcgcggcaga ttaattgaag atacattgga attgtatttt
     2701 gcagatgaga atcgatattt taaaggggat agtgtcgagt tagaggaaaa ggattttctt
     2761 acgaaaaacg taaaagagat tctgaattta gcagtaggga aatatgggtt agaaaaagag
     2821 aaattaacag attcatatcc acgaggtgat aaggtcgtct atagatttga ggatgaaagg
     2881 aaggtctggg aattgtgggt tgatgcaaag aaaaatagag ttgagaaagg gactttcttc
     2941 ctgaaagaca ataaggcaag ttataacttg aatttcaaaa gttactttga aaacaggggg
     3001 tttgagtttc cgcgtttaat agaaatttcc agctcctccg gggatgagta tatcaaacta
     3061 aaattcatcg agcgcaaagt gaattattcc attcctccta acagattcga tttgaaaatc
     3121 ccgcaggatg cggtccaaac ggatagtttt tgaggcgtta taccctaatc atctcctaat
     3181 tgggttcggg tggaggtaaa ctctacccct acgaaattta aatctcattt cgttcacttg
     3241 taggtgaccc atatgggtca cctgctgttt taacaacctc tccctaaccc cctcctttgt
     3301 gaggagaggg aacaagcgag gttctaaact ggctcgacga atctaacaac tgcaaataaa
     3361 gaagctatat ccgaagtgaa gggattattt gtaggggcgt aacgctatac gcccccacgg
     3421 actaccagat aaaagagatt gacaaaatcc ttttggatta ttagtataaa tttctgtatg
     3481 tatagaagag tcttaaaata cataaagccc tattggaagc atctggttgg ctcaatctta
     3541 gccactatcc ttttcgcaat cgcaagtggc gccctggtat ggttggtggg acccctgatg
     3601 gggactcttt tttccttgga ggggtattcg caagtcaact taaaccaggc tttggacagt
     3661 agcaaatcgg ttgaaaagga gttgactccg accctcacct ccctgaagga gactctaaaa
     3721 aatagcgcca accgactaat catcggcaag accaaatatg atactctgaa gaagttgtgc
     3781 attatcataa tttttg
//
